LOCUS       DBERYS010000001       104340 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.001, whole genome shotgun sequence.
ACCESSION   DBERYS010000001 DBERYS010000000
VERSION     DBERYS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 104340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.001"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            61..717
                     /locus_tag="ACL8LA_000001"
     CDS             61..717
                     /locus_tag="ACL8LA_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGS1811839.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            707..1030
                     /locus_tag="ACL8LA_000002"
                     /pseudo
     CDS             707..1030
                     /locus_tag="ACL8LA_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
     gene            1134..1964
                     /locus_tag="ACL8LA_000003"
     CDS             1134..1964
                     /locus_tag="ACL8LA_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1811840.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2118..2972
                     /locus_tag="ACL8LA_000004"
     CDS             2118..2972
                     /locus_tag="ACL8LA_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS1811841.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3072..4445
                     /locus_tag="ACL8LA_000005"
     CDS             3072..4445
                     /locus_tag="ACL8LA_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGS1811842.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            4438..5499
                     /locus_tag="ACL8LA_000006"
     CDS             4438..5499
                     /locus_tag="ACL8LA_000006"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGS1811843.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5501..6193
                     /locus_tag="ACL8LA_000007"
     CDS             5501..6193
                     /locus_tag="ACL8LA_000007"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGS1811844.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6222..6791)
                     /locus_tag="ACL8LA_000008"
     CDS             complement(6222..6791)
                     /locus_tag="ACL8LA_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS1811845.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6926..7540
                     /locus_tag="ACL8LA_000009"
     CDS             6926..7540
                     /locus_tag="ACL8LA_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS1811846.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7518..9188
                     /locus_tag="ACL8LA_000010"
     CDS             7518..9188
                     /locus_tag="ACL8LA_000010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1811847.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HIGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9200..10486
                     /locus_tag="ACL8LA_000011"
     CDS             9200..10486
                     /locus_tag="ACL8LA_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1811848.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10538..11071
                     /locus_tag="ACL8LA_000012"
     CDS             10538..11071
                     /locus_tag="ACL8LA_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1811849.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11149..11619
                     /gene="nrdI"
                     /locus_tag="ACL8LA_000013"
     CDS             11149..11619
                     /gene="nrdI"
                     /locus_tag="ACL8LA_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGS1811850.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11912..13174)
                     /locus_tag="ACL8LA_000014"
     CDS             complement(11912..13174)
                     /locus_tag="ACL8LA_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGS1811851.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13484..13942)
                     /locus_tag="ACL8LA_000015"
     CDS             complement(13484..13942)
                     /locus_tag="ACL8LA_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGS1811852.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(13939..14379)
                     /locus_tag="ACL8LA_000016"
     CDS             complement(13939..14379)
                     /locus_tag="ACL8LA_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1811853.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14735..16684
                     /gene="mutL"
                     /locus_tag="ACL8LA_000017"
     CDS             14735..16684
                     /gene="mutL"
                     /locus_tag="ACL8LA_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGS1811854.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17009..17476)
                     /gene="ribE"
                     /locus_tag="ACL8LA_000018"
     CDS             complement(17009..17476)
                     /gene="ribE"
                     /locus_tag="ACL8LA_000018"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGS1811855.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17477..18682)
                     /locus_tag="ACL8LA_000019"
     CDS             complement(17477..18682)
                     /locus_tag="ACL8LA_000019"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGS1811856.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18702..19337)
                     /gene="ribE"
                     /locus_tag="ACL8LA_000020"
     CDS             complement(18702..19337)
                     /gene="ribE"
                     /locus_tag="ACL8LA_000020"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGS1811857.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19322..20422)
                     /gene="ribD"
                     /locus_tag="ACL8LA_000021"
     CDS             complement(19322..20422)
                     /gene="ribD"
                     /locus_tag="ACL8LA_000021"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGS1811858.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20557..20671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            20828..21421
                     /gene="ruvA"
                     /locus_tag="ACL8LA_000022"
     CDS             20828..21421
                     /gene="ruvA"
                     /locus_tag="ACL8LA_000022"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGS1811859.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21431..21994
                     /locus_tag="ACL8LA_000023"
     CDS             21431..21994
                     /locus_tag="ACL8LA_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGS1811860.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            21991..22668
                     /locus_tag="ACL8LA_000024"
     CDS             21991..22668
                     /locus_tag="ACL8LA_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1811861.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(22805..23836)
                     /locus_tag="ACL8LA_000025"
     CDS             complement(22805..23836)
                     /locus_tag="ACL8LA_000025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGS1811862.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(24091..24774)
                     /locus_tag="ACL8LA_000026"
     CDS             complement(24091..24774)
                     /locus_tag="ACL8LA_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGS1811863.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(24786..25730)
                     /locus_tag="ACL8LA_000027"
     CDS             complement(24786..25730)
                     /locus_tag="ACL8LA_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS1811864.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            25862..28693
                     /gene="uvrA"
                     /locus_tag="ACL8LA_000028"
     CDS             25862..28693
                     /gene="uvrA"
                     /locus_tag="ACL8LA_000028"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGS1811865.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            28686..29747
                     /locus_tag="ACL8LA_000029"
     CDS             28686..29747
                     /locus_tag="ACL8LA_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS1811866.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            29879..30277
                     /gene="spx"
                     /locus_tag="ACL8LA_000030"
     CDS             29879..30277
                     /gene="spx"
                     /locus_tag="ACL8LA_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGS1811867.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            30343..30912
                     /locus_tag="ACL8LA_000031"
     CDS             30343..30912
                     /locus_tag="ACL8LA_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGS1811868.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            30999..31265
                     /locus_tag="ACL8LA_000032"
     CDS             30999..31265
                     /locus_tag="ACL8LA_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGS1811869.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            31269..31688
                     /gene="ruvX"
                     /locus_tag="ACL8LA_000033"
     CDS             31269..31688
                     /gene="ruvX"
                     /locus_tag="ACL8LA_000033"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGS1811870.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            31704..32009
                     /locus_tag="ACL8LA_000034"
     CDS             31704..32009
                     /locus_tag="ACL8LA_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGS1811871.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            32267..33517
                     /locus_tag="ACL8LA_000035"
     CDS             32267..33517
                     /locus_tag="ACL8LA_000035"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS1811872.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            33601..34056
                     /locus_tag="ACL8LA_000036"
     CDS             33601..34056
                     /locus_tag="ACL8LA_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGS1811873.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPVAQQVQQPAQQN
                     TNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESDG
                     DQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            34202..35734
                     /gene="cls"
                     /locus_tag="ACL8LA_000037"
     CDS             34202..35734
                     /gene="cls"
                     /locus_tag="ACL8LA_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGS1811874.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            35992..37530
                     /locus_tag="ACL8LA_000038"
     CDS             35992..37530
                     /locus_tag="ACL8LA_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGS1811875.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            37650..39857
                     /gene="nrdD"
                     /locus_tag="ACL8LA_000039"
     CDS             37650..39857
                     /gene="nrdD"
                     /locus_tag="ACL8LA_000039"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGS1811876.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            39869..40009
                     /locus_tag="ACL8LA_000040"
     CDS             39869..40009
                     /locus_tag="ACL8LA_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811877.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            40053..40559
                     /locus_tag="ACL8LA_000041"
     CDS             40053..40559
                     /locus_tag="ACL8LA_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1811878.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            40552..41142
                     /gene="nrdG"
                     /locus_tag="ACL8LA_000042"
     CDS             40552..41142
                     /gene="nrdG"
                     /locus_tag="ACL8LA_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGS1811879.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            41150..41767
                     /locus_tag="ACL8LA_000043"
     CDS             41150..41767
                     /locus_tag="ACL8LA_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS1811880.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            42033..42341
                     /gene="rpsJ"
                     /locus_tag="ACL8LA_000044"
     CDS             42033..42341
                     /gene="rpsJ"
                     /locus_tag="ACL8LA_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGS1811881.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            42558..43184
                     /gene="rplC"
                     /locus_tag="ACL8LA_000045"
     CDS             42558..43184
                     /gene="rplC"
                     /locus_tag="ACL8LA_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGS1811882.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            43209..43832
                     /gene="rplD"
                     /locus_tag="ACL8LA_000046"
     CDS             43209..43832
                     /gene="rplD"
                     /locus_tag="ACL8LA_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGS1811883.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            43832..44128
                     /locus_tag="ACL8LA_000047"
     CDS             43832..44128
                     /locus_tag="ACL8LA_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGS1811884.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            44146..44979
                     /gene="rplB"
                     /locus_tag="ACL8LA_000048"
     CDS             44146..44979
                     /gene="rplB"
                     /locus_tag="ACL8LA_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGS1811885.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            45083..45364
                     /gene="rpsS"
                     /locus_tag="ACL8LA_000049"
     CDS             45083..45364
                     /gene="rpsS"
                     /locus_tag="ACL8LA_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGS1811886.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            45376..45720
                     /gene="rplV"
                     /locus_tag="ACL8LA_000050"
     CDS             45376..45720
                     /gene="rplV"
                     /locus_tag="ACL8LA_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGS1811887.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            45733..46386
                     /gene="rpsC"
                     /locus_tag="ACL8LA_000051"
     CDS             45733..46386
                     /gene="rpsC"
                     /locus_tag="ACL8LA_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGS1811888.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            46390..46803
                     /gene="rplP"
                     /locus_tag="ACL8LA_000052"
     CDS             46390..46803
                     /gene="rplP"
                     /locus_tag="ACL8LA_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGS1811889.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            46813..47019
                     /gene="rpmC"
                     /locus_tag="ACL8LA_000053"
     CDS             46813..47019
                     /gene="rpmC"
                     /locus_tag="ACL8LA_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGS1811890.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            47044..47304
                     /gene="rpsQ"
                     /locus_tag="ACL8LA_000054"
     CDS             47044..47304
                     /gene="rpsQ"
                     /locus_tag="ACL8LA_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGS1811891.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            47330..47698
                     /gene="rplN"
                     /locus_tag="ACL8LA_000055"
     CDS             47330..47698
                     /gene="rplN"
                     /locus_tag="ACL8LA_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGS1811892.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            47776..48081
                     /gene="rplX"
                     /locus_tag="ACL8LA_000056"
     CDS             47776..48081
                     /gene="rplX"
                     /locus_tag="ACL8LA_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGS1811893.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            48105..48647
                     /gene="rplE"
                     /locus_tag="ACL8LA_000057"
     CDS             48105..48647
                     /gene="rplE"
                     /locus_tag="ACL8LA_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGS1811894.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            48665..48934
                     /gene="rpsN"
                     /locus_tag="ACL8LA_000058"
     CDS             48665..48934
                     /gene="rpsN"
                     /locus_tag="ACL8LA_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGS1811895.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            49219..49617
                     /gene="rpsH"
                     /locus_tag="ACL8LA_000059"
     CDS             49219..49617
                     /gene="rpsH"
                     /locus_tag="ACL8LA_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGS1811896.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            49809..50345
                     /gene="rplF"
                     /locus_tag="ACL8LA_000060"
     CDS             49809..50345
                     /gene="rplF"
                     /locus_tag="ACL8LA_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGS1811897.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            50429..50785
                     /gene="rplR"
                     /locus_tag="ACL8LA_000061"
     CDS             50429..50785
                     /gene="rplR"
                     /locus_tag="ACL8LA_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGS1811898.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            50803..51297
                     /gene="rpsE"
                     /locus_tag="ACL8LA_000062"
     CDS             50803..51297
                     /gene="rpsE"
                     /locus_tag="ACL8LA_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGS1811899.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            51311..51493
                     /gene="rpmD"
                     /locus_tag="ACL8LA_000063"
     CDS             51311..51493
                     /gene="rpmD"
                     /locus_tag="ACL8LA_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGS1811900.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            51638..52078
                     /gene="rplO"
                     /locus_tag="ACL8LA_000064"
     CDS             51638..52078
                     /gene="rplO"
                     /locus_tag="ACL8LA_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGS1811901.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            52091..53401
                     /gene="secY"
                     /locus_tag="ACL8LA_000065"
     CDS             52091..53401
                     /gene="secY"
                     /locus_tag="ACL8LA_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGS1811902.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            53552..54190
                     /locus_tag="ACL8LA_000066"
     CDS             53552..54190
                     /locus_tag="ACL8LA_000066"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGS1811903.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            54307..54525
                     /gene="infA"
                     /locus_tag="ACL8LA_000067"
     CDS             54307..54525
                     /gene="infA"
                     /locus_tag="ACL8LA_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGS1811904.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            54550..54666
                     /gene="rpmJ"
                     /locus_tag="ACL8LA_000068"
     CDS             54550..54666
                     /gene="rpmJ"
                     /locus_tag="ACL8LA_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGS1811905.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            54684..55049
                     /gene="rpsM"
                     /locus_tag="ACL8LA_000069"
     CDS             54684..55049
                     /gene="rpsM"
                     /locus_tag="ACL8LA_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGS1811906.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            55067..55450
                     /gene="rpsK"
                     /locus_tag="ACL8LA_000070"
     CDS             55067..55450
                     /gene="rpsK"
                     /locus_tag="ACL8LA_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGS1811907.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            55493..56428
                     /locus_tag="ACL8LA_000071"
     CDS             55493..56428
                     /locus_tag="ACL8LA_000071"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGS1811908.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            56440..56826
                     /gene="rplQ"
                     /locus_tag="ACL8LA_000072"
     CDS             56440..56826
                     /gene="rplQ"
                     /locus_tag="ACL8LA_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGS1811909.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            57091..57357
                     /locus_tag="ACL8LA_000073"
     CDS             57091..57357
                     /locus_tag="ACL8LA_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGS1811910.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            57367..58704
                     /locus_tag="ACL8LA_000074"
     CDS             57367..58704
                     /locus_tag="ACL8LA_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGS1811911.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDETDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            58948..59640
                     /locus_tag="ACL8LA_000075"
     CDS             58948..59640
                     /locus_tag="ACL8LA_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1811912.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(59743..61379)
                     /locus_tag="ACL8LA_000076"
                     /pseudo
     CDS             complement(59743..61379)
                     /locus_tag="ACL8LA_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(61369..62459)
                     /locus_tag="ACL8LA_000077"
                     /pseudo
     CDS             complement(61369..62459)
                     /locus_tag="ACL8LA_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(62472..63578)
                     /locus_tag="ACL8LA_000078"
                     /pseudo
     CDS             complement(62472..63578)
                     /locus_tag="ACL8LA_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(63810..64034)
                     /locus_tag="ACL8LA_000079"
                     /pseudo
     CDS             complement(63810..64034)
                     /locus_tag="ACL8LA_000079"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(64082..64858)
                     /locus_tag="ACL8LA_000080"
     CDS             complement(64082..64858)
                     /locus_tag="ACL8LA_000080"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGS1811913.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            64980..65726
                     /locus_tag="ACL8LA_000081"
     CDS             64980..65726
                     /locus_tag="ACL8LA_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1811914.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            65739..66719
                     /locus_tag="ACL8LA_000082"
     CDS             65739..66719
                     /locus_tag="ACL8LA_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGS1811915.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            66914..67234
                     /locus_tag="ACL8LA_000083"
     CDS             66914..67234
                     /locus_tag="ACL8LA_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1811916.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            67280..67588
                     /locus_tag="ACL8LA_000084"
     CDS             67280..67588
                     /locus_tag="ACL8LA_000084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1811917.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            67605..68903
                     /locus_tag="ACL8LA_000085"
     CDS             67605..68903
                     /locus_tag="ACL8LA_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1811918.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            69054..71492
                     /locus_tag="ACL8LA_000086"
     CDS             69054..71492
                     /locus_tag="ACL8LA_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGS1811919.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            71511..72179
                     /locus_tag="ACL8LA_000087"
     CDS             71511..72179
                     /locus_tag="ACL8LA_000087"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGS1811920.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            72197..73285
                     /locus_tag="ACL8LA_000088"
     CDS             72197..73285
                     /locus_tag="ACL8LA_000088"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGS1811921.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            73740..76241
                     /gene="leuS"
                     /locus_tag="ACL8LA_000089"
     CDS             73740..76241
                     /gene="leuS"
                     /locus_tag="ACL8LA_000089"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGS1811922.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            76435..77130
                     /locus_tag="ACL8LA_000090"
     CDS             76435..77130
                     /locus_tag="ACL8LA_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1811923.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            77142..77558
                     /locus_tag="ACL8LA_000091"
     CDS             77142..77558
                     /locus_tag="ACL8LA_000091"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1811924.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(78373..78465)
                     /locus_tag="ACL8LA_000092"
     CDS             complement(78373..78465)
                     /locus_tag="ACL8LA_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGS1811925.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            78599..79597
                     /gene="ruvB"
                     /locus_tag="ACL8LA_000093"
     CDS             78599..79597
                     /gene="ruvB"
                     /locus_tag="ACL8LA_000093"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGS1811926.1"
                     /translation="MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            79578..80150
                     /locus_tag="ACL8LA_000094"
     CDS             79578..80150
                     /locus_tag="ACL8LA_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HGS1811927.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            80381..81139
                     /locus_tag="ACL8LA_000095"
     CDS             80381..81139
                     /locus_tag="ACL8LA_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGS1811928.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            81148..81951
                     /locus_tag="ACL8LA_000096"
     CDS             81148..81951
                     /locus_tag="ACL8LA_000096"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGS1811929.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            81973..83232
                     /gene="rseP"
                     /locus_tag="ACL8LA_000097"
     CDS             81973..83232
                     /gene="rseP"
                     /locus_tag="ACL8LA_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGS1811930.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            83245..85098
                     /locus_tag="ACL8LA_000098"
     CDS             83245..85098
                     /locus_tag="ACL8LA_000098"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGS1811931.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            85198..86577
                     /gene="bglA"
                     /locus_tag="ACL8LA_000099"
     CDS             85198..86577
                     /gene="bglA"
                     /locus_tag="ACL8LA_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS1811932.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            86769..88577
                     /gene="glmS"
                     /locus_tag="ACL8LA_000100"
     CDS             86769..88577
                     /gene="glmS"
                     /locus_tag="ACL8LA_000100"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGS1811933.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            88696..89745
                     /locus_tag="ACL8LA_000101"
     CDS             88696..89745
                     /locus_tag="ACL8LA_000101"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGS1811934.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(89845..93642)
                     /gene="spuA"
                     /locus_tag="ACL8LA_000102"
     CDS             complement(89845..93642)
                     /gene="spuA"
                     /locus_tag="ACL8LA_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGS1811935.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDTHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVKIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            94058..94471
                     /gene="rpsL"
                     /locus_tag="ACL8LA_000103"
     CDS             94058..94471
                     /gene="rpsL"
                     /locus_tag="ACL8LA_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGS1811936.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            94491..94961
                     /gene="rpsG"
                     /locus_tag="ACL8LA_000104"
     CDS             94491..94961
                     /gene="rpsG"
                     /locus_tag="ACL8LA_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGS1811937.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            95385..97466
                     /gene="fusA"
                     /locus_tag="ACL8LA_000105"
     CDS             95385..97466
                     /gene="fusA"
                     /locus_tag="ACL8LA_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGS1811938.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            97570..101961
                     /locus_tag="ACL8LA_000106"
     CDS             97570..101961
                     /locus_tag="ACL8LA_000106"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGS1811939.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            102063..102326
                     /locus_tag="ACL8LA_000107"
     CDS             102063..102326
                     /locus_tag="ACL8LA_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGS1811940.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            102319..102597
                     /locus_tag="ACL8LA_000108"
     CDS             102319..102597
                     /locus_tag="ACL8LA_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGS1811941.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            102618..102770
                     /locus_tag="ACL8LA_000109"
     CDS             102618..102770
                     /locus_tag="ACL8LA_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811942.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            102792..104033
                     /locus_tag="ACL8LA_000110"
     CDS             102792..104033
                     /locus_tag="ACL8LA_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGS1811943.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            104043..104273
                     /locus_tag="ACL8LA_000111"
     CDS             104043..104273
                     /locus_tag="ACL8LA_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS1811944.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT        31059 a        19201 c        23291 g        30789 t
ORIGIN      
        1 taagacaata tgaggagaaa gatgaaagaa aaaggatttt aggagggggc gcaggcagcc
       61 atgccaacgg ctcttggcta tgtcagtatc ggcctggcct gtggaattat cggtgcgccc
      121 tatgtgacac ctgttgagat gggcttgatg agtctctttg tttatgctgg gagtgcccag
      181 tttgccatgt tggcactgat tgtggttcaa gctcctgtgg cagctattgc tatgacggtt
      241 tttctaatca atttgcgtct ctttttgttg agcttacacg catcgactta tttccgtcat
      301 accagtcttt ggcacaatat cggtatgtct agtatcttga cagatgagac ctatggcgtt
      361 ttgatgggcg aattagccca tacagataag gtcaatccga tgtggatgca cgggaacaat
      421 ctcaacagct atgtggcttg gtttgtggga acagtggtcg gaacggctct aggtgacctg
      481 ttgccaaatc cagaaatctt tggtttagat tttgccctgg ttgggatgtt tatcgggatt
      541 tttgcttcgc aattccagat gatgcaaaga cggatttctg tccgcaatct gctcattatc
      601 ttagcagttg ttgcagtgtc cttcttttta cttttgacag tggtgtctca gtcactagct
      661 gttctgtttg cgacgctact tggttgtagc atggggatgg ttttagatgg tcagtaagta
      721 tcttttatta gcagttattt tctctggtct tgtgacctgg attccccgta tgattccctt
      781 catcttggtc aagtataagg gcctgcctac gattgttgag cgttttttga agtttttgcc
      841 tgtttccatt atctttgcct tgattctttc aagagtagtg acaggtaagg ttgggaacct
      901 tcctcaaatc aaatgactgg acttcttagc agtctttcca acagcttggg tagcttttcg
      961 ctaccgcaat ctagtcggta cggttctctt tggagtagtc ttgattgccg tcttgcgttt
     1021 ggtcttttaa ataagtcata aagaaaactt atcacagaga tagatatcat ataatggcgt
     1081 aattactttt tttctgataa gattataagg tattctattt tggaggaaat gacatgaaaa
     1141 aaatcgttaa atactcatct cttgcagccc ttgctcttgt tgctgcaggt gtgcttgcgg
     1201 cttgctcagg tggtgctaag aaagaaggac aagcagctag caagaaagaa atcatcgttg
     1261 caaccaatgg atcaccaagg ccatttatct atgaagaaaa tggcgaattg actggttacg
     1321 agattgaagt cgttcgcgct atctttaaag attctgacaa atatgatgtc aagtttgaaa
     1381 agacagaatg gtcaggtgtc tttgctggtc ttgacgctga ccgttacaat atggctgtca
     1441 acaatcttag ctacactaaa gaacgtgcgg agaaatacct ctatgccgca ccaattgccc
     1501 aaaatcctaa tgtccttgtc gtgaagaaag atgactctag tatcaagtct ctcgatgata
     1561 tcggtggaaa atcgacggaa gtcgttcaag ccactacatc agctaagcag ttagaagcat
     1621 acaacgctga acacacggac aacccaacta tccttaacta tactaaggca gatttccaac
     1681 aaatcatggt acgtttgagc gatggacaat ttgactataa gatttttgat aaaatcggtg
     1741 ttgaaacagt gatcaagaac caaggtttgg acaacttgaa agttatcgaa cttccaagcg
     1801 accaacaacc gtacgtttac ccacttcttg ctcagggtca agatgagttg aaatcgtttg
     1861 tagacaaacg catcaaagaa ctttataaag atggaactct tgaaaaattg tctaaacaat
     1921 tcttcggaga cacttatcta ccggcagaag ctgatattaa ataatttctt gaaatcatcc
     1981 attctgagta agatgggtgg tttctcagtt tttagggata tagttttaaa ctatatatct
     2041 gactatctaa taacaatttg tgaaatcaaa caaattgtga gatactagta cggtattatt
     2101 tttaaggaga aagaatcatg aaaatcaaaa aatggcttgg tctagcagcc cttgctacag
     2161 tcgcaggttt ggctcttgca gcttgcggaa actcagaaaa gaaagcagac aatgcaacaa
     2221 ctatcaaaat cgcaactgtt aaccgtagcg gttctgaaga aaaacgttgg gacaaaatcc
     2281 aagaattggt taaaaaagac ggaattacct tggaatttac agagttcaca gactactcac
     2341 aaccaaacaa agcaactgct gatggcgaag tagatttgaa cgctttccaa cactataact
     2401 tcttgaataa ctggaacaaa gaaaacggaa aagatcttgt agcgattgca gatacttaca
     2461 tctctccaat ccgcctttac tcaggtttga atggaagtgc caacaagtac actaaagtag
     2521 aagacatccc agcaaacgga gaaatcgctg taccaaatga cgctacaaac gaaagccgtg
     2581 cgctttactt gcttcaatca gctggcttga ttaaattgga tgtttctgga actgctcttg
     2641 caacagttgc caacatcaaa gaaaatccaa agaacttgaa aatcactgaa ttggacgcta
     2701 gccaaacagc tcgttcattg tcatcagttg acgctgccgt tgtaaacaat accttcgtta
     2761 cagaagcaaa attggactac aagaaagcac ttttcaaaga acaagctgat gaaaactcaa
     2821 aacaatggta caacatcatt gttgcgaaaa aagattggga aacatcacct aaggctgatg
     2881 ctatcaagaa agtaatcgca gcttaccaca cagatgacgt gaaaaaagtt atcgaagaat
     2941 catcagatgg tttggatcaa ccagtttggt aataagaaac agggaggtgg gagagaaaat
     3001 tccacctctt gcttttgtat agagtataga ttgtaaagaa gactattcgt tcatagaaag
     3061 gtagagagaa tatggttttt cctagcgaac aagaacagat tgaaaaattt gaaaaggatc
     3121 atgtagtcca acattatttt gaggttttgc gtaccttgat ttctaagaaa tcagtctttg
     3181 cccagcaggt tggactcaag gaagtcgcaa attatctggg tgagattttc aagcgtgttg
     3241 gagctgaagt ggagattgat gagagctata cagcgccctt tgtcatggca catttcaaga
     3301 gttcgcgtcc agatgccaag accttgattt tctataacca ctatgacact gtgccagcgg
     3361 atggggatca ggtctggaca gaggatcctt ttacgctttc ggtccgcaat ggcttcatgt
     3421 atgggcgtgg ggttgatgac gacaagggtc atatcacagc tcgcttgagt gctttgagaa
     3481 aatatatgca gcaccatgat gatttacctg tcaatatcag ctttatcatg gagggagcgg
     3541 aggaatcggc ttcaacagac ctagataagt atttggaaaa gcatgcagac aaactccgtg
     3601 gggcggattt gttggtctgg gaacaaggga ccaaaaatgc cttggagcag ctggaaattt
     3661 ctggtggcaa taaggggatt gtgacctttg atgccaaggt aaaaagcgct gatgtggata
     3721 tccactcgag ttatggtggt gttgtggaat cagctccttg gtatctcctc caagccttac
     3781 agtctcttcg tgctgcggat ggccgtatct tggttgaagg cttgtacgaa gaagtacaag
     3841 agcccaatga acgagaaatg gccttgctag aaacttatgg tcaacgaaac ccagaggaag
     3901 ttagtcggat ttatggattg gagttgcctc tcttacagga ggagcggatg gcctttctaa
     3961 aacgtttctt tttcgagcca gcgcttaata tcgaaggaat ccagtctggt tatcaaggtc
     4021 agggtgttaa gactatttta cctgcagaag ccagtgccaa gctagaggtt cgtctggttc
     4081 cgggcctaga accgcatgat gttctggaaa aaattcggaa acagctagac aaaaatggct
     4141 ttgataaggt agaattatac tataccttgg gagagatgag ctatcgaagc gatatgagcg
     4201 caccagccat tctcaatgtg atcgagttgg ccaagaaatt ctatccacag ggcgtttcag
     4261 tcttgccgac gacagcgggg acaggaccta tgcatacggt ctttgatgcc ctagaggtac
     4321 caatggttgc attcggtcta ggaaatgcca atagccgaga ccacggtgga gatgaaaatg
     4381 tgcgaatcgc tgattatcac acccatatcg aattagtaga ggagctgatt agaagctatg
     4441 agtagagata ttatcaagtt agatcagatc gatgtgactt ttcaccaaaa gaagagaacc
     4501 attacagcgg ttaaggatgt gaccattcac atccaagaag gggatatcta cggaatcgtt
     4561 ggatattctg gagcaggaaa atcaaccctt gtacgggtga ttaatctctt gcaaaaacca
     4621 tctgcaggga aaattaccat tgacgacgat gtgatttttg acggcaaggt gaccttgacg
     4681 gcagagcagt tgcgtcgtaa acgtcaagat atcggaatga ttttccagca ttttaacctg
     4741 atgagccaaa agacagcaga ggagaatgta gcctttgccc ttaaacactc tgaactcagc
     4801 aaggaagaaa agaaggctaa agtagctaag ttgttggact tggttggttt ggcagatcgt
     4861 gctgaaaact acccttcaca actatctgga gggcaaaaac agcgtgtggc aattgcgcgt
     4921 gccttggcca atgatccaaa aatcttgatt tcagacgagt caacttctgc ccttgatccg
     4981 aagacaacca agcagatttt ggccttgttg caagatttga accaaaaatt aggcttgact
     5041 gttgtcttga ttacgcatga aatgcagatt gtcaaagaca ttgccaaccg tgtggcggtt
     5101 atgcaggatg ggcatttgat tgaagagggt agtgtgcttg aaatcttctc agaccctaaa
     5161 caacctttga ctcaagactt tatctcaaca gctacaggta ttgacgaagc catggtcaaa
     5221 atcgagaagc aagaaatcgt ggaacacttg tctgaaaaca gtctcttggt gcaactcaag
     5281 tacgctggat cttcaacaga cgagccactt ttgaatgaat tgtacaagca ttatcaagta
     5341 atggctaata ttctctatgg gaatatcgaa atcctcgatg gtactcctgt tggagaattg
     5401 gtggtggtct tgtcaggtga aaaagcagcg ttggcaggtg cccaagaagc cattcgtcaa
     5461 gcaggcgtac agttaaaagt attgaaggga ggacagtaag atggaatcat tgattcaaac
     5521 ctatttgcca aatgtctata agatgggttg gtctggtcag gcaggctggg gaacagctat
     5581 ctacctaacc ctctatatga cagttctttc cttcattatc ggaggcttct tggggctagt
     5641 ggcaggtctc tttctcgtct tgacagcgcc aggtggtgtc ttggagaata aagtcgtatt
     5701 ctggatttta gacaaaatta cctcaatttt tcgtgcgatt ccctttatca tcctcttggc
     5761 aatcttgtca ccactttctc acttgattgt taaaacaagt atcgggccaa atgcagccct
     5821 tgtcccactt tcttttgcag tctttgcctt ctttgcccgt caggtgcagg ttgtcttggc
     5881 tgaattggat ggcggtgtca ttgaggcggc tcaagcgagc ggagcgactt tctgggacat
     5941 cgtgggtgtt tacctatcag aaggtcttcc agatttgatc cgtgtgacga ctgtgacctt
     6001 gatttccctt gttggggaaa cagctatggc cggtgcggtt ggagctggtg gtatcggtaa
     6061 cgtagccatc gcttatggat ttaaccgcta caatcacgat gtgaccatct tggcaaccat
     6121 tattatcatt ttgattatct ttgcaatcca attcttggga gatttcttga ctaagaaatt
     6181 gagtcataaa taaaaagagc cgcgtggctc tttttaactg atcagatttt ctgtgcaaat
     6241 tttttactca aggcttgtcc aatcaaggca cccactaggg ctccgatgac aatacttgcg
     6301 ataaatagaa ggacagttcc agggtttgga gcgaccatga tacggtcgat atattcttgg
     6361 gattttcctc ttgccagaag agtagccata taggctttgg gcgcaatcca cataagcaag
     6421 attggtcctg ttgtactaaa ggcgaaaata atgaaagaaa ggaagttctt tgttttgtcc
     6481 ttgtattttc ctaaatgagc tacttcatct gctaggaggc cacagataat tccaggaagg
     6541 aaggaaccag caccgtgttt agttcccaag aaaaagaggg caatgacaag gccgatagtg
     6601 gtaatggctc caaagcgcgg aacttttgcg actaggatca tatagacgct accgccgaca
     6661 agggcagtaa aggcaggcgc ataaaacatg tttccagttt ggtcaaagag attgcccaaa
     6721 aggacaccaa tccccatgca gaggaagtaa agaactgcaa aaagcagtgt agttaagata
     6781 gatttcttca tgaattgtct cctgataatt ttttcacaat tctcattgta ccacggtttg
     6841 gtgggattgt aaatggatac tagaagtttt tgaaaacgct tgaaatatga tataatggtt
     6901 tcatagttat ttttagggga atatcatggg tagattttta gactttgtct ttaatcgttt
     6961 ctttttaggg atgattgcga cagccttctt ttggctatta actttagcag gagggattat
     7021 ccttggtcta gcgccggcta gtgccacctt gatgagctta tatgcagaac atggttatag
     7081 ctttcgggaa tacagtttga aggaggcttg gtctctttac aagcaaaatt ttgtctcaag
     7141 caacctgatt ttctatagct ttttaggtgt gggtctagtt ttgacctatg gtttgtatct
     7201 cttggtgcaa ttgcctcatc agaccattgt tcatttgatt gcgacccttt tgaatgtcct
     7261 agtagttgcc ctgatctttt tggcttatac agtatcttta aaattacaag tttattttgc
     7321 cttgtcctat cgaaatagtc tcaaattatc cttgattggc atctttatga gtctagcagc
     7381 tgtggccaag attctccttg ggactgtgct acttgtagca attggttact atatgcctgc
     7441 cctgctattt tttgtaggaa ttgggatgtg gcatttcttt atcagtgata tgttggaacc
     7501 tgtctatgaa atcatccatg aaaaattggc gacaaaatag aatgaagcag ttttggctac
     7561 atacgcttct aagaacctat agttcagtga tgatcattat cattgcgagt tttgcaatct
     7621 tactctctta cgctgactgg gattcacgtg aaaaggaagc ccagagagta gcccagcgtg
     7681 taactgctag aacagtgagt gaaattgaat attaccatag agagtcaacc cagatagctc
     7741 aggctttagt tgaaaaccaa gctcgtattg agggaatcta taaatacttt agtcttagca
     7801 tgccagacta tttttactgg caattagagc ggaaagcttc gccttatata tcagtctctc
     7861 tgtatgaaaa tgttgatgac ctctatgttc gaaatgattt tgtaactgga gtggccattg
     7921 cttttcaaga ttacaaggaa gtctatgttt ctactaaaga caaacgtagt ggagaaaaaa
     7981 tcagggctga ggatttcaaa ccagcaggaa atagttttgc cattccggtg tcagatccag
     8041 tgtcagatca agatttagga gtgatttaca tctccttgga tcctgctgtt ttataccatg
     8101 ccattgataa tactagaggt catactccga tggcagtaac agtgacctca ccttttgata
     8161 cggagatttt tcatattggt gagacagttg ataaggagag tgaaaattgg ctagttggct
     8221 taacttctca tggatatcag gttcaggtgg cagttcctaa aaactttgtt ttacaaggaa
     8281 cagtgactag ctctgctttg attgtgggtt tgagccttct ctttattgtc attctttatc
     8341 tgactttgag gcagactttt gctaattacc aaaagcaggt agtggattta gtagaatcca
     8401 ttcaagtcat tgctcaaggc gaagaggggc gtcggattga catttccgag aaagatcagg
     8461 aattactcct aatcgcggag acgaccaatg atatgttgga tcgattggaa aagaatatcc
     8521 atgatattta ccagttagag cttagtcaaa aagatgccaa tatgcgagcc ttgcaggcgc
     8581 aaatcaatcc tcattttatg tataatacgc tggagttctt gcgcatgtat gcagttatgc
     8641 agagtcaaga tgagttggca gatatcattt atgaattcag tagtctcttg cgtaacaata
     8701 tttccgacga aagagagacc ctcctcaaac aggaattaga attttgccgt aaatacagct
     8761 atctctgcat ggttcgctat cccaagtcca ttgcctatgg tttcaagata gatccagagt
     8821 tagagaatat gaagattccc aagtttacct tgcaaccgct ggtagaaaac tatttcgcgc
     8881 atggtgttga ccacaggcgg acagataatg tgattagcat caaggctctt aaacaggatg
     8941 gttttgtgga aattttggtg gtcgataatg gtagaggaat gtcggctgaa aagttggcaa
     9001 atatccgaga aaaattaagt cagagatatt ttgaacacca agccagctac agtgatcaaa
     9061 ggcagtctat cgggattgtc aatgtacacg agcgttttgt gctctatttt ggagaccgct
     9121 atgccattac tatagagtct gcagagcaag ccggtgttca gtatcgtatt acaattcaag
     9181 atgagtagaa agggagaaaa tgtataaagt attattagta gatgatgagt acatggtgac
     9241 agaaggtctg aagcgtttga ttccctttga taagtgggat atggaggttg tcgcaacagc
     9301 cagtcatgcc gatgaagctc tagaatatgt tcaggaaaat cctgtcgatg tcatcatttc
     9361 cgatgtcaat atgccagaca aaacagggct tgatatgatt cgggagatga aagagatctt
     9421 accagatgct gcctatatcc tgctctcagg ttatcaggag tttgattatg taaaaagagc
     9481 aatgaacctt agtgtggtgg actatttggt caagcctgtt gataaggtag agctgggaaa
     9541 tctgctggag aagattgcag gtcagctcgg cgagagaggg aagaaaagtc agactcttag
     9601 tcaagaatta gacgaggctg gatttgttag ttatttaggg gataaggaga attggtggat
     9661 aggtctatcc aaggaaaaac aaggttcctt caccattccc tactatgtct tgggccaaga
     9721 ctggcagatt ttcatttctg gccaacctct agatggttta gtcgttacac cttttgaagc
     9781 tccttatcaa gaacactttg aacgctggaa gctgaatgct gagaaaaccc tcttttacgg
     9841 ttctgtaaat ttgcagcagt ctgagagtct ctttgcctat tacgaaccga tttatagggt
     9901 tatcattcag ggaaatctca atcaaatcgt agaagagtta aatctcttgg agaaggtagt
     9961 tcttgaaaat acgccgcgtg ttccgattac taaacagctt tttattcagt ttgtcatgga
    10021 tgttttccat ttatttgaac atctcaaagc tgatgacctg acagacatcg tcaaaaccat
    10081 tcatgctatt caatccttcg atgaattggt ttcttatatc aaggaaactc tgatcagctt
    10141 tttcggtcaa taccgtatga atgaaaatgt ggtcagtgtg ctggaagtca ttggtcgtga
    10201 ttaccaaaaa gagctttccc tcaaggatat cagtaaggct ctctttatca atcctgttta
    10261 tctagggcag ttgattaagc gtgaaaccga ttcgactttt gcagagttac taaacaaaca
    10321 acgtattaag gctgcccaac aacttttgct ttcaactagt gacagcatcg aagatatttg
    10381 ttatgctgtt ggttacagta accttggata tttctataaa gttttccgaa aattgtgcgg
    10441 aaaatcgcca aaagcctacc gaaaacaggt agaaactata ctataagatt tgtattcctt
    10501 tacaaaaggt gctataatat tatcaggagg ttctatcatg aaaaagaaac caatttatct
    10561 atgggtcttg ctaatcttgt ctgcccttat ttcagttccg tctctgtttg gaattgtgag
    10621 tcccttgcct agtaaagaag cccttcgtgc tgctcagaaa caagttgcag gggtcaatgc
    10681 tcagcaatta gaagaccagc ttaattatac ctatagagta gcagaagcat ctcattctat
    10741 ttttaatgtt gctttgattg tgctatctac tattttagtg gtcgtagcga ttgtcttcct
    10801 tgttcgtaaa aatttgcaat atgcaaacta tacttacgtt ggctatgttt tgttagctat
    10861 tattggttcg atttatggct atgttggttt acaagacgct gtgcagttgg ttcaagatga
    10921 gagcatgcgt ttgacagtga gtattggttc aaaagctgtt agcattttct atatcgtcat
    10981 caatgttctc ttcctagccc ttgtcttcta taagatgtgg cgacaacaga aagccttggc
    11041 agaagaagag gaaacagaag agcttaccta agtattgaca aaaaagaact atcaagatac
    11101 aatcttggta gttgtttttt gattatagat aaaatcatgg aggtacatat gaagacaatt
    11161 tctttagttt atattagttt gagtggcaac actgaaagtt ttgtgacgcg cttgaaagac
    11221 tatctcttgt cccagtacaa agggattgag gttcaaaaga ttcatatcaa ggatttggtc
    11281 aaggaaggca aaaatttcta tgaaatggac catccctatg tcgctttttt gccgacctat
    11341 ctcgaaggtg ggaatggcgt ggataacgga gatgttgaga ttttgacgac accggtggga
    11401 gattttattg cctatggtaa caatgctagt aagtgttttg gtgtggttgg gtcaggaaat
    11461 cgtaacttta ataaccaata ctgcctaaca gccaagcaat acagtcaacg ctttggcttc
    11521 cctgtattgg ctgactttga aatgcgaggc atgctggaag atatcaaaca tgtcgcagct
    11581 attatcgcag atttgtatga gttggaaaag gagaattaat actcaatgaa aatcaaagag
    11641 caaactagga agctagtcgc aggttgctca aagcacagct ttgaggttgc agatagagct
    11701 gacgtggttt gaagagattc tcgaatagta taaagttctt ataagatgtg gttgaagttg
    11761 ttcaactgtt ttttgagtat aaacagtctt taagaacgta aaaaagcata agttcaggtc
    11821 ttcaaaacct tgttcttatg ctttttatca tagaaatatt tgctgaattt tctcttgaaa
    11881 tgaaacctta tcttcatctc gcgcttgtcc cttaccctgc ttttccgatt gcgagtgcgt
    11941 agataaagaa gatggcgaag ccaaagagga aaatcaatcc cgcaatggca aggtgtttga
    12001 gccaagaagg aagatttttg ttttcacgtg ttaccaaggc gtaattcaaa agagcaaaga
    12061 aaggtgttgt caggaaagag ccaatcatgg caaagcggag catggttgaa acctgaccag
    12121 cgaagaactt gataatgacg ataccgatga tagcagtgat ggtcatccag atgttcaaag
    12181 atttacgatt gtcctctttt tgactgatta gcagtcggag agattcctga ttaacgcgag
    12241 aatagccatc gataacagtt ataactgttc caaagataca gaggaaggca ataaaggtaa
    12301 tcaagtaacg ggaccattcg ccaagaacag aggcatacat gcccacgaat tgagagatgt
    12361 atttggctga agcagcttca accgcctgcc ctgtaggata ctgaatcagt gctcccagtg
    12421 ccacaaagaa gacggctagg atagctgttc caatataacc agtgttaaag tcaaacagag
    12481 cgtcttctgt gttaaagttg acggtctttc tcttttcagc tgaccaaagt gaattgatgg
    12541 ctgaaatttc aataggagcc ggcatccatc ctaggaggga gacgatgaag ggcagagctg
    12601 ccatttgcca aggtgtcttc tcgacaaaat cagaactgta ttctggatgc ttgaccgccg
    12661 caatgataac tgcaagaaca gtcgcaatgg ttaaggcaga cataatccat ttgaccatgc
    12721 cgtctaaaag tttgtagcct ccaaagagta gcatagccca aatgattgca acgagaatga
    12781 gggaccactg agtaatgcta agtccaatca ttgggaaggc actggcgatg atagctgagc
    12841 acagaatggc aacaccagcc gtgttgacca tagccgaaaa gacattgaga ataaagaaaa
    12901 tccagagata gagttttcct ttttcggcat aaccttcaac caaagtcttt ccagtatcag
    12961 ctgtgtattc agcaccaaaa cggaaaaatg gatatttaaa gacattggct aagatgacca
    13021 agagaagtag agaccaaccg taagaaccgc cagcttgagt tgaggataca atgtgggaac
    13081 ctccaacagc ggcagttgcc attaggattc caggtcccat tgcttttagc ttgcttgccc
    13141 aggttgattg atttaaggaa atagcttgcg acatgataaa tactcctttt tgaattttca
    13201 gaataatctg tatatgcaat ctatattcta caaaaaattt tggaaaaatg caagaatatt
    13261 ttgaaaaaag atagaaaatt tacaaagaaa atctgaaata actgttatga tatagcagat
    13321 tgaaactaga atagtacact tttgcttcta aaacattgtt agaaatcgat ttgactgtcc
    13381 tgattgattt atcctgttct tatttcattt cactatagta aggtgcagca ggataaagca
    13441 aaaccgagaa gtttctttct cggttttgct tattatgagg ttcctatttt ctatgctcca
    13501 agagttgatt gatgtgatct acttttttag cctctctttt atagagaata gcccagatga
    13561 tgagatagac aatcgcaaac tcgataatga gctggaggta gaaaatccag tggaagggga
    13621 accaaccagc aagagttgct agtgggacaa agccagccag cataaggaag aaatgagtca
    13681 gagtggcacg aagcatgctc cagtcacggc tgaataagcg gttgccaaag ttgaagagca
    13741 taccgatggc tgcccagata agtgtgcagt agagcaagac cagggcaccg tgaacctgat
    13801 gctgatccat cacttggccg atgagagagt agggatttag tggtgcgtag gtatttggtg
    13861 cataaatgag tgaaaagagg atggagagga tgaggccgat aagaacaccg gcagctgcat
    13921 cgtgaaatac ttgttttttc atagttctaa tttctccttg atggttttta gataacggcg
    13981 tgaagagtag gtgaagcttt cgtttttcaa gaaaatttct accagaccgt ttggcgtgag
    14041 cttgaggtga gagatggaat cgatattgat gatttctgat tgggaaattt ggataaaatt
    14101 gcttggcaag agtttaagaa cctgatagag tcgcaaatca atgctgtagg tctgactcgc
    14161 ggtttctgct agaaccttcc gattctcgat atagaagcgc tgaatcttgc caatctcaac
    14221 tagatagacc tgatcatcga tttttccttt gattttttct ctttggtcca gattttctgc
    14281 gaactcgatg actttctgga ctttttcggt ttcttgaggt gcttggacaa tcagcttttc
    14341 ctcctcgtaa gtctcactaa tctgtagttc tactttcata aatttctctc cttgtttttc
    14401 atacaaaatt aatctcactt cttgtacacc tttattaata ctcttcaaaa atctcttcaa
    14461 accacgtcag cgtcgtcttg ccgtaggata gttgttgact ttgtcagttt tatctacaac
    14521 ctcaaaacag tgttttgagc tgtctgcgtc tagctttcta gtttgctctt tgatttttat
    14581 tgagtatacc actattttac tccctctggc aagggacttt gtctatgtgg agggattggg
    14641 ctcctatgtg gtggagtttt tctgttcttt ctgaaatatg gtataatagc actaattaat
    14701 ttctaggaaa atagatacag aaaggggctg aaagatgtct catattattg aattgccaga
    14761 gatgctggca aaccaaatcg cagctgggga ggtcattgaa cgtcctgcca gtgtggttaa
    14821 agagttggta gaaaatgcca ttgacgcggg ctctagtcag attatcattg agattgagga
    14881 agctggtctc aagaaggttc aaatcacgga taacggtcat ggaattgccc acgatgaggt
    14941 ggagttggcc ctgcgtcgcc atgcgaccag taagataaaa aatcaagcag atctctttcg
    15001 gattcggacg cttggttttc gtggtgaagc cttgccttct attgcgtctg ttagtgtctt
    15061 gactctgtta acggcggtgg atggtgctag tcatggaacc aagttagtcg cgcgtggggg
    15121 tgaagttgag gaagtcatcc cagcgactag tcctgtggga accaaggttt gtgtggagga
    15181 tctctttttc aacacgcctg cccgtctcaa gtatatgaag agccagcaag cggagttgtc
    15241 tcatatcatt gatattgtca accgtctggg cttggcccat cctgagattt cttttagctt
    15301 gattagtgat ggcaaggaaa tgacgcggac agcagggact ggtcaattgc gccaagcaat
    15361 cgcagggatt tacggtttgg tcagtgccaa gaagatgatt gaaattgaga actctgacct
    15421 agatttcgaa atttcaggtt ttgtgtcttt acctgagttg actcgggcta accgcaatta
    15481 tatcagcctc ttcatcaatg gccgttatat caagaacttc ctgctcaatc gtgctatttt
    15541 ggatggtttc ggaagcaagc ttatggttgg acgttttcca ctggctgtca ttcacatcca
    15601 tatcgaccct tatctagcgg atgtcaatgt gcatccaact aagcaagagg tgcggatttc
    15661 caaggaaaaa gaactgatga ccctggtttc agaagctatt gcaaatagtc tcaaggaaca
    15721 aaccttgatt ccagatgcct tggaaaatct tgccaaatcg accgtgcgca atcgtgagaa
    15781 ggtggagcaa actattctcc cactcaaaga aaatacgctc tactatgaga aaactgagcc
    15841 gtcaagacct agtcaaactg aagtagctga ttatcaggta gaattgactg atgaagggca
    15901 ggatttgacc ctgtttgcca aggaaacctt ggaccgattg accaagccag caaaactgca
    15961 ttttgcagag agaaagcctg ctaactacga ccagctagac catccagagt tagatcttgc
    16021 tagcatcgat aaggcttatg acaaactgga gcgagaagaa gcatccagct tcccagagtt
    16081 ggagtttttc ggacaaatgc acgggactta tctctttgcc caagggcgag atggacttta
    16141 catcatagat cagcacgctg ctcaggaacg ggtcaagtac gaggagtacc gtgaaagcat
    16201 tggcaatgtt gaccagagtc agcagcaact cctagtgccc tatatctttg aatttcctgc
    16261 ggatgatgcc ctgcgtctca aggaaagaat gcctctctta gaggaagtgg gcgtctttct
    16321 agcagagtac ggagaaaatc aatttattct acgtgaacat cctatttgga tggcagaaga
    16381 agagattgaa tcaggcatct atgagatgtg cgacatgctc cttttgacca aggaagtttc
    16441 tatcaagaaa taccgagcag agctggctat catgatgtct tgcaagcgat ctatcaaggc
    16501 caatcatcgt attgatgatc attcagctag acaactcctc tatcagcttt ctcaatgtga
    16561 caatccctat aactgtcctc acggacgtcc tgttttggtg cattttacca agtcggatat
    16621 ggaaaagatg ttccgacgta ttcaggaaaa tcacaccagt ctccgtgagt tggggaaata
    16681 ttaaaagtat aaaaaagtct gggaaaaatt ttcaaaatca gaaaaacgca taaaatcagg
    16741 tgttcaaaaa ccttgatttt atgcgtttta tcatggaaat agttacttca tgttttccta
    16801 attcttttcg aaactctttt taaaccacgt cagttttatc agtaatctca aaacagtgtt
    16861 ttgagctaat tttgccagtt ttgtctgtaa catcgaagtt gtgttttacc actctgcgac
    16921 tggtttccta gtttgctcta tgattttcac agagtattaa attgcgattt tgccaagttt
    16981 ctttattcgt ctaaaagtag agtctgttct atgcgtctaa tgtacgaatt aggttgacca
    17041 tttcaatagc tccttgtgca cactcagaac ccttatttcc tgctttggta ccagctcgtt
    17101 ctatggcttg ttcaattgta tctgtcgtta gcacaccaaa cataacagga atttcgctat
    17161 ttaaactgat ttgggcgatt cccttagata cctcgctaca tacataatca taatgacttg
    17221 tattccctct aatgacagct cccaagcaga taattgcatc atatttttta ctttttgcca
    17281 tttttgatgc aatcagtggt atttcaaaag ctcctggaac ccaggctacc tctatatctt
    17341 tctcgtttac attctctctt ttgagattat ctagtgctcc agataataat tttgaagtta
    17401 taaattcatt aaatctcgct acaacaatac ctattttaat attgtttgct actaaattac
    17461 cttcataagt gttcatttat ttttcctcca tatttaaaat gtgacccatt cgatttttct
    17521 ttgtttctaa ataaaaacta tcgtaaggat tggcttctat ttcgattgat attctactgg
    17581 aaatggtaat tccatatttt tctaactgtt caaccttgtc aggattattt gtcagtaaat
    17641 gaagtgactg aagtcccaga tctttaagca tttgtgctcc aatatgatat tctcttaaat
    17701 caccttcaaa gcctaatgca agattggcat caagcgtatc catgccttga tcttgtaaat
    17761 gataggcttt taatttattg ataagtccaa ttcctcgtcc ctcctgtcgc aagtaaagta
    17821 agacacccga accattctca acaatcattt tcatagcttt atcgaattgc tgtccacaat
    17881 cgcaacgtaa agagcctaaa acatctcctg ttaaacattc ggagtggacc cgacataata
    17941 cattggcttc atcctctatg tttcccataa taagagcaag atgatgttcc ccatttagtt
    18001 tatctatata gctaattgct ttgaaattac cgtatctagt aggcatattg acagttgaaa
    18061 ctcgttctat cagctgatca tatacttttc tatattcttg taattctttg atggtaatta
    18121 gtggaatgtt gtgtttcttc gagaactgaa ttaaatcatc tgttctcatc attttgccat
    18181 catgattcat tatttcacaa cataggccac actcttttag tccagctaat tttaataaat
    18241 caacagttgc ttctgtgtgt ccatttcttt ctagaacacc accttttttt gcaattaaag
    18301 gaaacatgtg tcctggcctg cgaaaatcag agggtgttat atcttcagct acacacatac
    18361 gtgcggtcag tcctctttcc tcggcagaaa tacctgtggt cgtttcttta taatcaattg
    18421 aaactgtaaa agcagtctta tgattatctg tattgttttc aaccataggt gaaagcatta
    18481 attgattagc taaactttcg ctcataggca tacaaattaa tcctttggca taagtagcca
    18541 taaaattaac attttctgtt gtagctgctt gtgcagaaca aattaagtct ccttcatttt
    18601 ctctatcctt gtcgtctata acaagaacaa gtcgtccctt ctgcaatgct tctaatgttt
    18661 cttgtatttt tcgatattcc attgactgat tatcctttct gctaaaatcc attttgatat
    18721 aatagttcct tagatatttc tgattttgga gagttatcca tcagtttttg cacatattta
    18781 cctaagatat cattttcaag atttactgta ctcccgactt gtttactttt aagaatggtt
    18841 tgtttcaagg tatgagggat aacagatact gaaaagttta ctttggagac tttagcgaca
    18901 gtcagactaa tgccgtcaat tgtaatagat cctttttcaa ctattaaatc taaaatttct
    18961 ttttgtgtgt tgatttgata ccatacagca ttatcatctt tttttattga cgagattttt
    19021 cctgtaccat caatgtgtcc tgtaacgacg tgacccccaa gtcgaccgtt gatagataag
    19081 gctctttcta gattcacctc acttccatgt tttaatagag taagagctgt tcgactccat
    19141 gtttcattca ttacatcaac tgtaaaggat tgatgattga aatgagtaac tgtaagacag
    19201 ataccattta ctgctatact atcgcctaaa tggatatccg ttaatatttt tgaggcttta
    19261 attgatagtt tacaattacg agagtctttc tgtattcttt caacttttcc gatttcttca
    19321 attattcctg tgaacatgga taaatcactt cactttctat gagatagtca tttcctattt
    19381 gagaaaaagc ataaggtttc aatctaatag cgtcatttgg caaagaaatg ccttcacctc
    19441 cgacaggaaa cttggcactg cctccaaaaa tttttggtgc aatatatatt ttcagctcat
    19501 caacaatttg ttgttccaaa gcactccaat tcattagact gcccccttct agaactaggc
    19561 tatcaatctg catgtttcct agatgttgca ttaaactcga taagtctata tgattgcctt
    19621 ttttctttat ggaaagtatt tcacagccat gattttgata tagcttcatt ttatttttgt
    19681 cttcagagga agtggcaatg taagttttaa tatcatttgc tgtttttacg attttagagg
    19741 taagaggagt tcgtaaatgt gtatcgcata tgatacggat aggatttttc ccttcctcca
    19801 atctacatgt cagcaaagga tcgtcttgaa taacagtatt gactcccacc ataattgcac
    19861 taacatggtg tcgtaactga tgcacatgct ttcttgcttc ttcttcagta atccatttgg
    19921 attgatttgt tttagtggct atttttccat ccattgacat tgcatatttc ataaaaacat
    19981 agggtacatg ctgggtaata tactttctaa aactttttat taagttaaga cactcatttt
    20041 ctaaaattcc aacagtaact tgaagattat tttcctcaag tatctttact ccttttccag
    20101 atacaatagg attacagtct aggcttccaa tgactactct tgtaatacca ctatcgatta
    20161 tagcatctat acagggaggt gttttcccga agtgacaaca gggttcaagt gttacataaa
    20221 gcgtcgctcc gacaggggat tctctacagt ttttaagagc atttctctca gcatgtgggc
    20281 caccaaaaaa ctcatgataa ccttgtccga tgatgtgatt atcttttaca ataactgcgc
    20341 cgaccatagg attgggattg acgtaaccag cccctttttg tgccagtttt attgctaatt
    20401 tcatatattt tgaatcgctc atctcactac ctccaaaaaa aatatacctt gaatagggga
    20461 ctactcaagg catacaaaag aaaacttatg cgattaacaa aaatactctg aaatgacaag
    20521 taatcatttc agagcacgca aaaagcacaa atatactttt atcttctttc atccagacta
    20581 tactgtcggc tttggaattt caccaaatca tgcctttcgg ctcgtgggct ataccaccgg
    20641 tagggaatta caccctgccc tgaagatagt tattcaatta cagatgatta tagtacttaa
    20701 ttttgaatat gtcaacagat aaataccgat tgtttttgat atactgtatt tgtgataatc
    20761 gattctcgct cctcggataa agaaaatatg atatactaga taaacgaaat aagagagaag
    20821 gaatactatg tacgcatatt taaaaggaat cattaccaaa attactgcca aatacattgt
    20881 tcttgaaacc aatggtattg gttatatcct gcatgtggcc aatccttatg cctattcagg
    20941 tcaggttaat caggaggatc agatttatgt gcatcaggtt gtgcgtgagg acgcccattt
    21001 gctttatgga tttcgctcag aggatgagaa aaagctcttt cttagtctga tttcggtctc
    21061 tgggattggt cctgtatcag ctcttgctat tatcgctgct gatgacaatg ctggcttggt
    21121 tcaagccatt gaaaccaaga acatcaccta cttgaccaag ttccctaaaa ttggcaagaa
    21181 aacagcccag cagatggtgc tggacttgga aggcaaggta gtagttgcag gagatgacct
    21241 tcctgccaag gtcgcagtgc aagcaagtgc tgaaaaccaa gaattggaag aagctatgga
    21301 agccatgttg gctctgggct acaaggcaac agagctcaag aaaatcaaga aattctttga
    21361 aggaacgaca gatacagctg agaactatat caagtcggcc cttaaaatgt tggtcaaata
    21421 ggagcagaga atgacaaaac gttgttcgtg ggtcaagatg acaaacccgc tctacatcgc
    21481 ctatcatgat gaggagtggg gtcagcccct ccatgatgac caagcattgt ttgagttgtt
    21541 gtgtatggaa acctatcagg caggcctgtc ttgggaaacg gtactcaaca aacgccaagc
    21601 tttccgagaa gcctttcata gctatcaaat tcactcagtc gcagagatga ctgagactga
    21661 attggaagcc atgctggaga atccagctat cattcgaaat agagccaagc tttttgctac
    21721 acgcgctaac gcccaagtcc ttctacagtt acaggcagag tacggctctt ttgatgccta
    21781 tctttggtct tttgttgagg ggaaaattgt cgttaacgat gttcctgatt atcgccaagc
    21841 gccagctaaa acacccttat ctgagaaatt agccaaagat ctcaaaaaac gaggcttcaa
    21901 gttcacaggc ccagtcgccg tattgtcttt tctacaggct gcagggctag ttgatgacca
    21961 cgagaatgat tgtgagtgga aaggtcttaa atgatgtcta acaaaaataa gggaattctg
    22021 atttttgcga ttctctatac agtcctcttt gtgtttgatg gcgttaaatt gctggcttct
    22081 ttaatgccat ctgtcattgc aaattatctt gtttatgtag ttttagcttt atatggctcc
    22141 ttcttgttca aggatagatt gatccaacaa tggaaggaga ttagaaagac taaaagaaaa
    22201 ttcttctttg gcgtcttaac aggatggctc tttctcattc tgatgactgt tgtctttgaa
    22261 tttgtatcag agatgttgaa gcagtttgtg agactagatg gacaaggtct aaatcagtct
    22321 aatattcaaa gtacctttca agaacaacca ctactgatag ctgtttttgc ttgtgtcatt
    22381 ggacctctgg tagaagaatt atttttccgt caggtcttat tgcattactt gcaggaacgg
    22441 ttgccaggtt tactaagcat tattctggta ggacttgttt ttgctctgac tcatatgcac
    22501 agtttggccc tatcagagtg gattggtgca gttggttact taggtggagg ccttgccttt
    22561 tctattattt atgtgaaaga aaaagagaat atctactatc ccctacttgt tcacatgtta
    22621 agcaacagcc tctccttaat cattttagct atcagtatag taaaatgaaa tgagaacagg
    22681 acaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatatac
    22741 tgtaatatgt gatgaaaatg ccagtaatga taccgagaaa aaagctgaga aacttttccc
    22801 agctttattt gttatagtca aagagaatga cttgttcctg tgcatctaca tgagcatgga
    22861 ccccaaaggg tacaattgct cttggagttg cgtggccgac attcagatta tagacaatcg
    22921 ggatattgct gtcaatgata tccaatagtg cctctttata gtcgtcatgg aaagtttcat
    22981 ccataggttt tccgaccaag agtccattga tgaccgcgaa tatgccagtg tcctttaaag
    23041 ttagcaacat ctttttgaag tcttctggct taggcttttc ttcgcttgtt tcgagcaaga
    23101 ggattttccc ttcccagtct gacaagtcag ggaaaagttt gtatttttgg cagagttccg
    23161 tgctatctgc gtatcgagag ttgtcaaaga tatcgtagag ggattcgagg caaccaccga
    23221 ggattttccc ctcgaactgg gcacttcctt gcaacaagtc aaaacctgta tttgtatgac
    23281 tgacacgagg tgttcccagg gccgtgggac taaaatcagt tcgttcctca taccaaacgt
    23341 cactagggcg gatttctgaa attcttcccg tctcaatcaa ttctttaaag tagtgaaggc
    23401 tataggctag catttctttg tctaattcac aaatgtctgc taaaaaggat tgaccataaa
    23461 aagtcttgat tcctaattta tgcaacatga ggtggttcat ggttgtatcc gagaagccaa
    23521 gaaaaatttt ttgtttgata accttttgga gttggtcatt ttcaaaaaga taaggtagca
    23581 agcgataggt atcgtctcca ccgatggcac ataggatcat gtcgatgcta tcatcagaaa
    23641 aggcatgaat caaatcctct gcacgagctt caggatggtc cttgataaag tctaatcctt
    23701 ttaacgaatg gggcaaaaag atgggattga gtcccagatc cttgagacgt tggataccca
    23761 agtccacttc gtgtttgaca aaatcctctc cgataatgcc actagataaa ctaacaatac
    23821 caatagtaga aaccatatct catcctccta gaaatagatt gagcctattt tatcacaaaa
    23881 tataagagaa agctatgtgg gatttcagaa gaatactttt aatactcttc gaaaatctct
    23941 tcaaaccacg tcagttttat ttgcaacctc aaagctgtgc tttgagcaac ctgcagctag
    24001 cttcctagtt tgctctttga ttttcattga gtataactca aaaaagtaag tgaaaaagca
    24061 accgcacctg cagttgcttt tatcgttttc ttattcttga tagcgtgttg gtcctgcact
    24121 tgcactacgg atattcaagc gtttcttgag atagaagcga agggctagga gggctactcc
    24181 aataatagct agtggcaatg gatccagtac tgggttaagg ctagctggta ggaagcttgt
    24241 tgcaaagaag acaagcaacc aaaggaacat agaagttagg ataactagta cagatttcca
    24301 gaaaggtgga cgttgactgc gatccatatc tggtccatag tattggtaaa caaagtagta
    24361 catcaagtag aaggcaaatc caccaaccag tccaactaat agaagagtaa tcaatccata
    24421 gccgaaagct tgatctgccg caaagaaggt tgtgagggcg ctgacaaggg caaagaggct
    24481 agtgatgaaa agagctgagt ccataatcat cagttttgga tcatcatttt cttttggatg
    24541 ctctttttcg tactgctctt tgacagtgaa gctatgagcc caatgagttg gtgcgccgta
    24601 tagggaacgg gcagttgtac ctttagattg ctcctcaagg atttggggaa tgacttcctc
    24661 aaaaatagtc tggatttcag cgtctgtttt cccatctttg atgaattgtt gggtagcaat
    24721 gtggacaaac tcttggtttt tcttagttaa tttttgtaga tcaatttgag acataggaac
    24781 tcctcttaga accatttttt atggatgaga tagagagtga gcgagacact catagcaaag
    24841 gcgataaaga cgattaacca gaaggcattt ggctctccgt ttaggggaat ttcattatcc
    24901 ttaaagttca tcccgtaggc agaaaagacc atggttggga tggacatgac gatggtcaca
    24961 agggccaagg ttttcatgat gttgttctgg ttgttagaaa tgatagaggc aaaggtctct
    25021 gtcatagaat gcaagacgtt tccataaata tctgccatct cgatggcctg ttgggtttca
    25081 atcagggtgt cttcaagcag gtcttcgtcc tcaaggtatt tcttgatatt gctggttgaa
    25141 ctggtcaatt tcttaatcac gcgctcattt gttttgaggg aggccttgaa atagacgata
    25201 gttttttcca attccatgag ctcaatcaat tcttcattac gagttgattg atgcagttga
    25261 ctttcgattt gttcactctt gcggtcgatt gaacgaaggg ctgttaggta aagctctgca
    25321 ttgcgataaa gaatttgaaa gataaaacgt gaacgcatga aggtatagaa attacgcaat
    25381 cgacggttga taaagacatc aaggacaggt agtggttcca aacacgtagt gataatggtt
    25441 tcctcagtga tgataatacc aagcgggatg gttacgtagt aggtgcggtt atttctttcc
    25501 tccgtgaccg gcacgtctac gataatcagg gtatactcgt cttcaatggt aatacgagac
    25561 atttcttccg catcgagcgg tgctcgaagg tcggcaatat caatatcgaa ggtgttggcg
    25621 atttcgagtg attcattttg agtcggattg acgagattga tccaagtacc cggttcaagc
    25681 gtatcgatct ctttaaattc agttgttgta gagagaaaaa cttgtttcat atcccttatc
    25741 ctttctcatt tttcagattt tttcacactg tactattata ctacaaaatc ggccttttgg
    25801 gtaatagaat ctcacttttt ttggtataat ggtaagcaat aatggactag aaagaacaaa
    25861 gatgcaagat aaaattgtca ttcatggggc gcgtgcccat aatttaaaaa atattgatgt
    25921 ggagattccg cgagacaagt tggttgtcgt gactggcttg tcaggttcag ggaagtccag
    25981 tctggctttt gatactctct atgcggaggg acaacgtcgc tatgtagaga gtttgtctgc
    26041 ctacgctcgt cagttcttgg gaaatatgga aaaaccagat gtagatgcca ttgatggtct
    26101 cagcccagct atttccatcg accagaaaac gactagtaaa aatcctcgct cgacggtggg
    26161 aacgacgact gaaatcaatg actatctgcg tctcctctac gcacgtgtgg ggacgcctta
    26221 ctgtatcaac ggacatggag ctatcaatgc ttcttctgtg gagcaaatcg ttgataaggt
    26281 tttggagtta cctgaacgcc agcgcttgca gatcttggct cctgtcatcc gcaagaaaaa
    26341 aggccaacat aagagtgtta tcgagaaggt tcagaaagac gggtatgttc gtgttcgtgt
    26401 ggatggggaa gtctatgatg tgaccgaagt gccagagttg tctaagagca agcaacacaa
    26461 tatcgatgtc gtggttgacc gtattgttat caaggagggc attcgtagcc gtctctttga
    26521 ttccattgaa gctgcccttc gtatcgcaga aggttatgtg attatcgaca ccatggacga
    26581 ctctgagttg ttgttctctg agcattatgc ctgtccagtt tgtggattta ctgtcccaga
    26641 gttagaacct cgtctcttct ctttcaatgc tccctttggc tcttgtagtg agtgtgacgg
    26701 cttggggatt aagctggagg tggatactga tttggtagtg ccagatgcca gcaaaacctt
    26761 acatgaggga gcgctggcac cttggaatcc tatctcatcc aactactatc caaacatgtt
    26821 agagcaggcc atgaaagtct ttggagtggc tatggataag ccttttgagg acctgtcaga
    26881 agaagataag aacttgattc tctacggctc agatggtaag gaattccatt tccattatga
    26941 aaatgaattt ggtggtgtgc gcgatatcga cattccgttt gagggagtta tcaataatat
    27001 caaacgtcgt taccatgaaa ccaatagcga ttacactcgc actcagatgc gtctctacat
    27061 gaatgagctg acctgcggaa cctgtcaagg ctatcgtctc aatgaccagg ccttgtctgt
    27121 ccgtgtgggc ggccagcaag ggccacatat tggagaaatc tcagacctgt ctatcgctga
    27181 ccacttggac ttggtgagcc agttaacttt gtctgaaaat gaagccatca ttgctcgtcc
    27241 cattctcaag gaaatcaagg atcgtttgac cttccttaat aatgtgggtc ttaactatct
    27301 aaccctgtca cgttcagcag gaaccctttc aggtggggaa agtcagcgca ttcgtttggc
    27361 aacccagatt ggttccaacc tatcaggtgt cctctatatc ctagacgagc cgtcaatcgg
    27421 tcttcaccag agggacaatg accgcctgat tgccagtctg aaaaagatgc gtgacttggg
    27481 caatactctt atcgtggtgg aacacgacga agataccatg cgtgaagctg attatctgat
    27541 tgacgttggt cctggtgccg gtgtttttgg tggtgagatt gttgcagcag gtacacctaa
    27601 gcaagtggct cgcaacagca agtctatcac aggtcagtac ttgtcaggca aacgtgtcat
    27661 cccagtacca gaagagcgcc gtgtcggaaa tggtcgtttt attgaagtga caggagcgcg
    27721 tgagaacaac ttgcaaaatg tcacggctcg ctttccacta ggaaaattta tcgcagtgac
    27781 aggtgtgtcg ggttcaggga aatcgactct aatcaacagc attctcaaaa aagccattgc
    27841 ccagaagctc aaccgtaatt cagacaaacc tggtaagttt aagactatta cagggattga
    27901 gcatgtcgac cgtttgattg atattgacca gagccctatc ggacggacgc cgaggtctaa
    27961 cccagctacc tatacaggag tttttgacga tatacgtgac ctttttgctc agacaaatga
    28021 agccaagatt cgaggctaca agaaggggcg cttcagtttc aacgtcaagg gaggtcgctg
    28081 tgaagcttgc tcaggtgacg ggattatcaa gattgagatg cacttcttgc cagatgttta
    28141 tgtggcttgt gaagtttgcc acggaacccg ctataacagt gaaaccctag aagttcacta
    28201 caaggaaaag aatatttcgc aggtcttgga tatgacggtc aacgatgcgg tagaattttt
    28261 ccaacacatt ccgaaaattc aacgcaaact ccagaccatc aaggatgtag gtctaggcta
    28321 tgtgaccttg ggacagccag ctaccaccct ttctggggga gaagcccaac gtatgaagtt
    28381 agctagcgaa ctccacaaac gctcgacagg aaaatctttc tacattctgg atgagccgac
    28441 gacagggctt catacagagg acattgctcg cctgctcaag gttttagctc gctttgtaga
    28501 cgccggcaat acagtcctcg tcatcgagca caatctggat gttatcaaga cggcagacca
    28561 tatcattgac ttgggacctg agggcggtgt cggtggtgga accatcatcg taacaggaac
    28621 tccagaagaa gtagctgcca acgaagccag ctatacagga cactatttga aaggaaagtt
    28681 acatcatgaa taaacgcgta caagcatttc tagctaaaat gcaagaaaaa gaactagatg
    28741 gtatcatcat caacaatctt aaaaacgtct attatttgac tggtttttgg ggctcaaacg
    28801 gaacagtctt tatcagccgt gaccgtcagg tcttagtgac agactctcgc tatatcatcg
    28861 cagctaagca agaaaccagt ggttttgaga ttgtggctga tcgtgatgaa ttggctgtca
    28921 ttgcaggaat tgttaaggac atgaacttga ctcgtatcgg ttttgaagat gagatttcag
    28981 tgtcttatta ccaccgtatg caggcagctt ttgcaggttt gaacttgctt ccacaaactc
    29041 agtttgtgga aggtcttcgt atgattaagg atgaggcaga gattgcagcg attcgcaagg
    29101 cttgttctat ctcagaccaa gctttccgcg atgcgcttga ctttatcaaa ccaggaaaaa
    29161 ctgaaattga gattgccaac ttccttgatt tccgcatgcg tgagttggga gcatctggct
    29221 tatcttttga tacgatccta gctagcggta tcaattcttc taaaccccat gcccatccaa
    29281 tgcacaaacc agtggagttg ggagaagcca ttaccatgga cttcggctgt ctctatgacc
    29341 actatgtcag tgatatgacc cggactatct atctagggca tgttagcgat gagcaggcag
    29401 agatttacaa tacggttcta aaagctaacc aagccttgat tgaccaagct aaggcaggct
    29461 taggtttccg tgactttgac aaaatccctc gtgatattat cattgaggca ggttatggtg
    29521 actactttac tcacggcatt ggccacggta ttggtctgga tatccatgag gaaccctact
    29581 ttagtcagac ttctacagaa actattaaga caggtatggc cttgaccgat gaaccaggta
    29641 tctatatcga aggcaaatat ggcgttcgta tcgaggatga tatcctgatt acagagacag
    29701 gttgtgaatt attgacccta gctccaaaag agttgatagt catttagttg tttgtcaaga
    29761 aaaaagtacg aaaatgacga aaaaagtcaa aaaaaattaa aaataggtcg caagtcggat
    29821 gttttttatg gtataataga ctaaactgat agtaacatgt agcgaaaggg gtaggtacat
    29881 gattaaaatt tatacagtct caagttgtac tagttgtaaa aaagcaaaaa cctggctcaa
    29941 tgcccaccag ttaagttata aagaacaaaa ccttggtaaa gaaggaatta cgagagaaga
    30001 attactggat attctaacca aaacagataa cggaatagcc agcatcgttt cgtctaaaaa
    30061 tcgctatgcc aaagcccttg gagtggatat tgaagatttg agtgtcaatg aagttctcaa
    30121 tctgattatg gaaacaccga gaattttaaa gagcccaatc cttgtagatg aaaaacgcct
    30181 gcaagttggc tacaaggaag acgatattcg tgccttccta ccacgctctg tccgtaatgt
    30241 agaaaatgca gaagcacgtt tgcgtgcagc tctataaaca tcaaggctgg gagcacttcc
    30301 cagtcttatt ctattttaat ttcaaaaaga aagaagaaag aaatgaaaaa aatagttctt
    30361 gttagtctag ctttcctttt tgtcctggtt ggttgcggac agaaaaaaga aactggacca
    30421 gctacaaaaa cagaaaaaga tacgcttcag tcggcattgc cagttattga aaatgctgag
    30481 aagaatacag ttgtaactaa gactttggtc ttgcccaagt cagatgatgg tagccagcag
    30541 acacaaacaa ttacttacaa agacaagact tttttgagtc tagctatcca acaaaaacgt
    30601 ccagtctctg atgagttgaa gacttatatt gaccaacatg gagtggagga aactcaaaaa
    30661 gctcttcttg aagcggagga gaaggataag tctatcattg aagctcgtaa attggcaggt
    30721 ttcaaacttg aaacaaaact attgagcgca acggaacttc aaacaacgac tagttttgat
    30781 tttcaagttc tggatgtcaa gaaggcttcc cagttggaac atctgaagaa tattggtttg
    30841 gaaaatcttt tgaaaaatga accaagcaaa tatatttcag atagattggc aaatggcgcg
    30901 acagaacaat agaaaatcca aataaataga ctgcctgaat tgtagaaggt aaggaattat
    30961 gctataatgg tactattcta aggaaagagg gtgttagaat gggatttact gaagaaacag
    31021 tacgttttaa attggacgat tccaataaaa aagaaattag cgaaactttg acagatgttt
    31081 atgcttcgtt gaacgataag ggttacaacc caattaacca aatcgtaggt tacgtattga
    31141 gtggagaccc tgcctacgtt cctcgttata ataatgcacg aaatcaaatc cgtaagtatg
    31201 agcgtgatga aatcgttgag gaattggttc gctactacct caaaggacaa ggagtcgatc
    31261 tataacctat gagaattatg ggattggacg tcggttcaaa aacggtaggg gtggcgatta
    31321 gcgatccgct tggttttaca gctcaagggc ttgaaatcat ccagataaat gaagaacaag
    31381 gccaatttgg ttttgaccgc gttaaggaat tggttgatac ttacaaggtg gaacgatttg
    31441 tagtgggctt gcctaaaaac atgaacaata caagtggacc gcgcgtagaa gctagtcaag
    31501 cctacggagc aaagctagaa gagttttttg gtttaccagt agactatcag gatgaacgct
    31561 tgacaacagt ggctgctgag cgcatgttga ttgaacaagc agatatcagt cgcaataagc
    31621 gcaagaaagt cattgataag ttagcagctc agctgatttt acaaaattat ttagatagaa
    31681 aattttaata taaaggagag gctatgtcac acgatcataa ccacgaccac gaagaacgtg
    31741 aactaatcac actagtagat gagcaaggaa atgaaacctt gtttgaaatc cttttgacaa
    31801 ttgatggaaa agaagaattt ggtaaaaact atgttcttct agtaccagtt aacgcagaag
    31861 aagacgaaga cggacaagtt gaaatccaag cttactcatt cattgaaaat gaagatggaa
    31921 cagaaggcga attgcaacca atcccagaag actcagaaga cgaatggaac atgattgaag
    31981 aagtcttcaa cagctttatg gaggagtgat acagtctggg agactgtatc agcccaactc
    32041 cagaaatggg gaagagttgg aacgtccagt gaacattttt accccaactc ttagaaattg
    32101 agaagagttg gaacatctag tggatgtttt tagcccatat tcacattcat agtagctagt
    32161 tattagctaa ccaggtaaga ggtcgggacg aaaatcctgg ccttgttttt attagtaacc
    32221 atactttaat gcgataattg attggacttt tgtcaaggag atgtgtatgt ttgaagtaga
    32281 agaatggctc catagtcgga ttggtttgaa ttttcgatca ggtttgggcc gaatgcagca
    32341 agcggtggat ttgttaggaa atcctgagca gtcttaccct attatccacg taacagggac
    32401 taatgggaaa ggatctacca ttgcttttat gagggaatta tttatggggc atggcaaaaa
    32461 agttgcgacc tttacctccc ctcatatcgt ctctatcaat gaccgaatct gcattaatgg
    32521 gcaacctata gcagacgcag actttatccg tttgactgat caggtcaagg agatggagaa
    32581 aacgcttctg caaactcctg cccagttgtc cttttttgaa ttgctgacct tagttgcttt
    32641 tctttatttt agggagcaag aggtggattt ggttttatta gaagtgggaa ttggtggctt
    32701 acttgacacg accaatgtgg taactggaga gcttgctgtc atcacctcca ttgggcttga
    32761 ccatcaagaa accttgggtg atagtctaga agcaattgca gagcagaaag ctggtatttt
    32821 caaggctggt aaaaaggcag tgattgcgaa attgcctcca gaagctagac ttgtctgtca
    32881 gaaaaaagcc gaatctttag ctgttgacct ttatcaggca ggtcaagatt tttcagtgct
    32941 gaatggtgat ttttcaagct ctttactaaa tctttcacag ctgaacatag gcttagaagg
    33001 agcctatcag caggagaatg cggccttggc gttgcaaact tttcttcttt ttatgggaga
    33061 aagaaaggaa gctattgatg aacaggctgt aagaaaggcc ttggaacaga cccattgggc
    33121 tggtcgcttg gagcgtattc gcccacagat ttacttggat ggtgctcata acctccctgc
    33181 cttgactcgc ttggttgagt ttatcaaaga aaaagagcag gaaggctatc gacctcaaat
    33241 cctctttgga tccttgaaac gtaaggatta tcaagggatg ttgggttatc tgactgaaaa
    33301 tttacctcaa gtggaactca aaatgaccgg ctttgactat caagggtctt tggacgagac
    33361 agatgtaaca ggttaccatg taattccctc ttaccgagaa tttatcagca gtttcgaaga
    33421 aagggcagac gctcaagact tgctgttcgt tacagggtct ctctatttta tctcagaagt
    33481 acggggctac ctgctggacc gtgagcagat aaattgaccc ccttttttga acttgttata
    33541 ctaggggaat tgctggttag agaaaatttc tctaaattgg tagcagaaag gaaattcatc
    33601 atgaaattaa aaagattcac actttctctt gcttctctag caagttttag tctcttagta
    33661 gcttgttcac aaagagctca acaggttcaa cagcctgttg ctcagcaggt ccaacagcct
    33721 gctcaacaga ataccaatac tgcaaatgca ggaggtaacc aaaatcaagc ggctccagta
    33781 caaaaccaac ctgttgctca accgaccgat attgatggga cttatactgg tcaggatgac
    33841 ggagaccgta tcactttagt ggtaactgga acgactggta catggactga gctcgaatct
    33901 gacggggatc agaaagtcaa acaggttaca tttgattcag caaatcaacg catgattatt
    33961 ggcgatgatg tcaaaattta cactgtaaac ggtaatcaaa tcgtcgtaga tgatatggat
    34021 agagacccat cggaccaaat cgttttaact aaataagact atgcaagtag aaagggctgg
    34081 atttttcagc ctttttactt ttacctagaa aataatcata aatacttgcc ttctaccgaa
    34141 tacctgcgtt atactagtat caattacttg tttttagcat tcaaactatc aaggagggga
    34201 tatgaaatat aggaaatttc aattattgat gtccaagtat ggctttagtc tttcgattat
    34261 gctacttgaa ctttgtcttg tttttggtct ctttctttat ttaggacgca tggctcccat
    34321 tttatggatt actgtcctca ttctactgag tatcatcaca atcatttcga tagtcaaccg
    34381 taatacgact cctgagaata aggtaacctg gttgttagta gcctttgtgc cagtatttgg
    34441 tcccttgctc tatctgatgt ttggtgaaag gcgattgtcc aaaaaagaaa tcaaacaact
    34501 gaagaagcta ggctctatgc atttccaaga agcaaatagc cagctactaa aagagaaatt
    34561 aaaagaaagt gacaaggcag cttatggagt catcaagtcc ttattgagta tggataccaa
    34621 tgctgacatc tatgatcaaa ctgcctctac attttttcct aacggagaag ctatgtggaa
    34681 aaagatggta gaagatctta aaaaggctga gaaatttatt ttcttggaat attacattat
    34741 agaagaaggt ttgatgtgga atcgcatact agatatacta gagcaaaagg tagctcaggg
    34801 tgtagaggtt aagatgctct atgatgatat cggctgtatg gctactttaa caggagatta
    34861 tgcacatcga cttcgtcagc tgggcatcga ggcccataaa ttcaataaag ttattcctcg
    34921 tttgacagtg gcttataata acagagatca tagaaaaata ttgattgttg atggtcagat
    34981 agcctatact ggtggggtca atctggcaga tgagtacatt aaccacgtcg agagatttgg
    35041 ttattggaag gatagtggaa ttcgcttaga cggactagca gtaaaagccc tgacacgctt
    35101 atttttgacc acttggtaca ttaatcgagg agaaattagt gattttgatc aatatcattt
    35161 agaaaatcat tctatcccga gtgacggttt aaccattcca tacggaagtg gacccaagcc
    35221 aatttttcga gcgcaggtag ggaaaaaagt ttatcagagt ttaatcaatc aagcaacaga
    35281 atcggtctat attacgacac cttatttgat tatagattat gatttaacag agacaatcaa
    35341 aaatgcagct atgagagggg tcgatgttcg aattatcacc ccttacatac cagataagaa
    35401 gttcattcag ttagtcacga gaggagctta tcccgacctt ctttctgctg gtgttcggat
    35461 ttatgagtat agtccaggtt ttattcatag taagcagatg ttggtagacg aagattttgc
    35521 ggtggtgggg acaatcaatc tcgactaccg gagcttggta caccattatg aaaatgcagt
    35581 cttactctat aaaactcctt ctataaggga aatcgcccga gattttcgaa atatatttgc
    35641 agattctcag gaagtctatc ctcattctat caaaacgagc tggtatcaaa agcttgtaaa
    35701 agaaatcgcc cagctattcg cccctatctt ataagaaatc tagaacttag gatacaaacc
    35761 agacctattt ttcttgtctt ttactaatag aaagaaagag gaggggaatt catgctaact
    35821 aaggaagaag tgaatgatat gattgagttc aaacagacac atttataccg ctttcgggaa
    35881 attgaaacct ttgtgaaact atgcaaaaaa tcactcaaac agccatcgca agctgacaat
    35941 cctaggactt tttgatatac taagacagat aaattgaaca ggagaaacga tatgagtgtg
    36001 tatggtagag tagaagaagt tcataaggag aatcgtgaac ctctagaata ccaaatcgaa
    36061 caagaatcgc atcatcgtga atcaagtcgt cttcctttgg tgaaaatttt actatggagt
    36121 acgcttgtaa cggggataac tctaggagta ccgctattac tcgacttaat gagtgcacaa
    36181 gaagtgcagg atttttatgc aggttggggg cttcatcaga cagggaagat ttacagcgac
    36241 tattatggaa gtcaaggttt gctttattat ttgctgactt acgtgagtca gggcggattt
    36301 ttctttgcca tctttgagtg gttagccttg gtagcaggag gatttttcct ttttagatca
    36361 gcggacacct tgacagagca aggagaccaa gctggacatc tggtgactat tttttacatg
    36421 ctagttacag gtcttgcttt tggtggaggc tatgcgactc ttttagcgct tcctttctta
    36481 ttcgcagcct ttagtttagt tgcggcttac ctaagcaatc caagccatga taagggattt
    36541 gtacggattg ggctagcttt ggcaggcgga tttttctttg ctcccttatc atcgctcctg
    36601 tttattgctg tagtgagttt aggcttgttg gtctttaacc ttgggcatag acgctttgcg
    36661 catgggtttt atcagtttct tgcagtggct ttaggttttt cacttgtctt ttatccaact
    36721 gcctactata gtgctgcaac aggaagtttt ggggatgcga ttagtggtat tcgttatcct
    36781 attgacagta ttcgctttga ttttacttct aaaattttag agaatatgtt tttttatggc
    36841 ttgttgtccc ttggtttggg atttgtggtt tgtatctttt taggtctctt tcaatccaaa
    36901 ccctttaaac tatacgtcat ttcggttcct gcaagtcttg tggtaatttt aggtttgatt
    36961 ttacttttct tttcacaaga gcctctgcac gcttcttatt tgatggtcgt cttccctgtt
    37021 ttcctacttt tattggtaac caatattaag agtcaacaga gggggcgtag tgctagaaga
    37081 agccgaagag aaacgccagt tagcctatgg agtcgtttct tcaaaggaaa tctatatctg
    37141 ctagtttttg ggtttgtcta tcttttgtct gttccttttt tgatgaagtt tgtcctttat
    37201 ccagtacctt atcaagaacg taatcgtctt gctgatttgg taaaagagga gacaaatacg
    37261 gaagatgcta tctatgcatg ggatgatact gcgactcttt atcgtaagag tgagcgcttg
    37321 tcgccatcgg cgattttgtc cccgttgcac tatacagcaa ctgaggaaaa tcgtaataag
    37381 ttacttaatg acttgaaaga aaaacaacct aaggtgattg tggtaaatga caaggtggta
    37441 gtctggtctg aagtggaaac acttttaaaa gaaaattacc aacaagtaaa gactgattac
    37501 tcagagttta aagtctataa aattaaataa ccaaatcaat atcttgtgta tttttaaaaa
    37561 ttttaggatt tttaacacaa gatattgatt tttcttttta gagtggtata atacttttta
    37621 gaaagaacat tttagaaaag agcatgcgta tgattgcact agaagaaaaa attacaattt
    37681 tgccaactct cttcgtcgag aaacgagatg ggagacgtgt tgtatttgat gtggacaaga
    37741 ttgacaaggc tctccacaag gcggctgaca aggttatgga tgtgacaccc ctggttgaaa
    37801 aacgcctcaa tgatctgact gagcgaatta ttacagaaat tcatagtcgc tttccacagg
    37861 gaattaagat ttacgaaatt caaaatatcg tagaacatga actccttgaa gccaaagaat
    37921 atgcgctggc tgaggagtat attacttatc ggacacagag ggattttgag cgctcaaaag
    37981 cgacggatat caactttagt attcataaac ttctcaacaa agaccagaca gttgtcaatg
    38041 aaaatgctaa taaagacagt gatgtcttta acactcagcg tgatttgaca gcagggattg
    38101 ttgggaaatc aatcggactg caaatgcttc ctaagcacgt agccaatgcc caccaaaagg
    38161 gggatatcca ctatcacgat ttggactaca gtccctatac ccctatgacc aactgctgtt
    38221 tgattgattt taagggtatg ttggaaaatg gttttaagat tggaaatgca gaggtagaga
    38281 gtcccaagtc tatccagact gcgacagcac agatttctca aatcattgcc aacgttgctt
    38341 ctagccagta cggtggctgt tcagctgacc gtatcgatga aattttggcg ccttatgcag
    38401 agaagaatta tcaaaaacat ctcaaagatg cagaagagtg ggtattgcct gaaaaacggg
    38461 aagattacgc ttggaagaaa acgcaaaagg acatctacga tgccatgcaa tctcttgagt
    38521 atgaaatcaa tactctcttc acttcaaatg gacaaacacc ttttacttcg ttaggttttg
    38581 gtctgggaac cagtcgtttt gaacgagaaa ttcaaaaagc tattttaaac attcgcatca
    38641 agggtcttgg ttcagaacac cgtacggcta tctttcctaa acttattttc actctgaaaa
    38701 gaggccttaa tttagaggaa ggaactccca actacgacat caagcagttg gctcttgagt
    38761 gtgcaaccaa acggatgtat ccagatgttt tatcttacga taagattgtt gacttgacag
    38821 gatccttcaa ggtgcctatg ggttgccgtt ctttcctcca aggatggaag gatgaaaatg
    38881 gtgtagaagt caattcaggt cgcatgaatc tgggtgttgt gacggttaat ctgcctcgta
    38941 ttgctcttga gtctgaaggt gatatgaata agttctggga aatcttcaac gagcgaatga
    39001 atatcgcaga agatgctctt gtttaccgtg tcgaacgcac taaagaggcg acaccagcaa
    39061 atgctcctat tctttatcag tacggtgctt ttggccatcg tctaggtaaa gaagaaagtg
    39121 ttgaccagct ctttaagaat cgtcgtgcta ccgtttcgct gggctatatc ggcttgtatg
    39181 aagtagcgac agttttcttt ggtaacagct gggaaagtaa tccagatgct aaggaattca
    39241 cgctagacat cattcacgat atgaaacgcc gtgtagaaga gtggtcagac caatatggct
    39301 accatttctc tatctactca acaccatccg aaagtctgac agaccgtttc tgtcgactag
    39361 atatagacaa gtttggctct attcctgata tcacagacaa ggaatactac accaactctt
    39421 tccactacga tgttcgtaaa aatccaacac cgtttgaaaa attggacttt gagaaagtct
    39481 atccagaagc aggtgcgtca ggtggtttca tccattattg tgagtatcca gtccttcagc
    39541 aaaatccaaa ggccttggaa gctgtctggg attatgctta tgaccgtgta ggctatctag
    39601 gcaccaatac tccgattgac cgttgctaca agtgtgactt tgaaggggat tttgaaccaa
    39661 ctgagagagg gtttgcttgt ccaaactgtg gcaatagcga ccctaaaaca gtagatgtgg
    39721 tgaagcgaac ttgtggctac ctaggtaatc ctcaagcaag accgatggtc aacggccgtc
    39781 acaaggaaat cgctgcgcgt gtcaaacata tgaatggttc aacgattaaa atagctgggc
    39841 atcaagtaac aaattagaaa gaaatgaaat gggaaaatat caactagacg ataaggggcg
    39901 cgcacaagtg acccgttatc acgagaaaca ctctaaaggt ggagctggta agaaagaacg
    39961 cttgcttagc ttcagagaac aatttttaaa caagaacaag aaaaaataaa agtgagagcc
    40021 agctctcgct tttctcatag tgggaggtaa ggatggaatt acgcagacca agattagcgg
    40081 ataagaaagc tgttttagat atgatgacag agtttgaaaa atttcagtcg cctcacgacg
    40141 gcggtttctg ggatacagag aactttgtgt atgaagactg gttagaaagc aatcaggaac
    40201 aggaaatggg gattaatctg cctgaaggat gggttcctgc aattcagtta gtggcttttt
    40261 ctgagaaagg tcaagcagtt ggatttctta atctccggtt gcgcctcagt aactttctac
    40321 tagaagaagg tggccacatt ggctactcca ttcgtccatc tgaaagaggc aagggttatg
    40381 caaaagagac tctccgtcag ggcttgcaag ttgctaagga aaagaacatc aagaaagctc
    40441 tggtgacctg tagcgtgaat aatcctgcta gcagagcagt cattctagca aatggtggaa
    40501 tatttgagga tgctcgcaat ggagtcgagc gttattggat agaggtagcg aatgaataat
    40561 ccaaaaccac aagaatggaa aagcgaggaa cttagtcaag gtcgtatcat tgactacaag
    40621 gcctttaact ttgtggacgg cgaaggcgtg cgtaactctc tctatgtatc aggctgtatg
    40681 tttcactgcg agggatgtta taatgttgca acttggtctt ttaatgctgg cattccctat
    40741 acagcagaat tagaagagca gatcatggca gatcttgctc agccctatgt tcaaggtttg
    40801 actttgttgg gaggggagcc ttttcttaat actgggattc tcttgccact tgttaagcgg
    40861 attcggaagg aattgccaga caaggacatc tggtcctgga ccggctacac ttgggaagaa
    40921 atgatgttgg aaactccaga taaactggaa ttcttgtcac tgattgacat tcttgtcgat
    40981 ggaagatatg atcgaactaa gagaaatctt atgctccagt ttcgaggttc atctaaccaa
    41041 cgaattatcg atgtgcaaaa atcgctcaaa agtgggcaag tagtgatttg ggacaagctc
    41101 aatgacggaa aagaaagcta tgaacaggtg aagagagaat gaagaaaaaa tgaagaaaaa
    41161 agacttagta gaccaactag tctcagagat cgagacgggg aaagtcagga cactgggaat
    41221 atacggtcat ggagcttcag gtaaatcaac ctttgcacag gaattgtacc aagctttaga
    41281 ttctactaca gtaaatttgc tagaaacaga tccctatatc acctccggac gctatttggt
    41341 agtacccaag gacgcgccga atcaaaaggt gacagccagt ctgccagtgg cgcatgaact
    41401 ggagagtttg cagagagata tccttgcctt gcaggcgggt atggatgtct tgacaattga
    41461 agaaccttgg aaggctagtg aggtcttgtc tggagccaaa ccaattttga ttgtcgaagg
    41521 gatgtctgtt ggttttctac ccaaggaact ctttgaaaaa accatctgtt tctacacgga
    41581 tgaggagacc gaattaaagc gacgccttgc tagagatacg actgtgagaa atcgctatgc
    41641 atcctttata ttggctagcc atcagatgag acgggagcag tatctgcgat actataaaga
    41701 aactgagtct aaggcggata tcttagtgga ccaatcagaa gataaatttg atgtcaagag
    41761 gacttaactt atgtatagaa gaaatataga agaaaatccc taattttaga aagaaaaagt
    41821 aggaaaacag tttcatccca aaaaaacgaa aaaacctaac aaatcccttg caatcgcagg
    41881 ggatttgtgt tattctatta tggtgctgta aattacagcc ttagttttga tgcaagaggt
    41941 tgcgacacgc tcggttgcat tgccacgcaa caccgcgtcg gttttcttgt ggagctagcc
    42001 tattatctta aatagacgaa aaggagaaaa agatggcaaa caaaaaaatc cgtatccgtt
    42061 tgaaagctta cgaacaccgt acgcttgaca cagcggctgc aaaaatcgta gaatcagcta
    42121 ctcgtacagg tgcacaagtt gcgggtccaa tcccacttcc aactgaacgt agcctctaca
    42181 caatcattcg tgcgactcac aaatacaaag actctcgcga acaatttgaa atgcgtacac
    42241 acaaacgttt gatcgatatc gttaacccaa ctcaaaaaac agttgatgcc ttgatgaaat
    42301 tggatcttcc aagtggtgta aacgtagaaa tcaaacttta atctaaaata taaaagagca
    42361 gaggctggtg tttcaatcta attgaacacg ggctaaactc ggtgtgaaaa agataaactt
    42421 cctcgcgtct gctagacact gtgtcagttt cctattttca ctttgagttt gacgcccttt
    42481 gtatcttaga cttgagcata aaaaacgctc gttaaaaact ttttgaataa aaaatataga
    42541 aaaggaacta ttttctcatg acaaaaggaa tcttagggaa aaaagtggga atgactcaaa
    42601 tcttcactga agctggcgaa ttgatccctg taacagttat tgaagcaact ccaaacgttg
    42661 ttcttcaagt taaaactgtt gaaacagacg gatacaacgc tatccaagtt ggtttcgatg
    42721 acaaacgcga agtattgagc aacaaacctg ctaaaggaca tgtagcgaaa gctaacacgg
    42781 ctcctaagcg cttcattcgt gaattcaaaa acgttgaagg cttggaagtt ggtgctgaaa
    42841 ttacagttga aacattcgca gctggagacg ttgttgacgt aactggtact tctaaaggta
    42901 aaggtttcca aggtgttatc aaacgccacg gacaatcacg tggaccaatg gctcacggtt
    42961 ctcgttacca ccgtcgtcca ggttctatgg gacctgttgc acctaaccgc gtattcaaag
    43021 gtaaaaacct tgcagggcgt atgggtggcg accgcgtaac aattcaaaac cttgaagttg
    43081 tacaagttgt tccagaaaag aacgttatcc ttatcaaagg taacgtacca ggtgctaaga
    43141 aatctcttat cactatcaaa tcagcagtta aagctggtaa ataataaaga aaggggaaat
    43201 cagtcacaat ggcaaacgta acattatttg accaaactgg taaagaagct ggccaagttg
    43261 ttcttagcga tgcagtattt ggtatcgaac caaatgaatc agttgtgttt gatgtaatca
    43321 tcagccaacg cgcaagcctt cgtcaaggaa cacacgctgt taaaaaccgc tctgcagtat
    43381 caggtggtgg acgcaaacca tggcgtcaaa aaggaactgg acgtgctcgt caaggttcta
    43441 tccgctcacc acaatggcgt ggtggtggtg ttgtcttcgg accaactcca cgttcatacg
    43501 gctacaaact tccacaaaaa gttcgtcgcc tagctcttaa atcagtttac tctgaaaaag
    43561 ttgctgaaaa caaattcgta gctgtagacg ctctttcatt tacagctcca aaaactgctg
    43621 aatttgcaaa agttcttgca gcattgagca tcgattctaa agttcttgtt atccttgaag
    43681 aaggaaatga attcgcagct ctttcagctc gtaaccttcc aaacgtgaaa gttgcaactg
    43741 ctacaactgc aagtgttctt gacatcgcaa atagcgacaa acttcttgtc acacaagcag
    43801 ctatctctaa aatcgaggag gttcttgcat aatgaatttg tatgatgtta tcaaaaaacc
    43861 tgtcatcact gaaagctcaa tggctcaact tgaagcaggg aaatatgtat ttgaagttga
    43921 cactcgtgca cacaaacttt tgatcaagca agctgttgaa gctgctttcg aaggtgttaa
    43981 agttgccaat gttaacacaa tcaacgtaaa accaaaagct aaacgtgttg gacgttacac
    44041 tggttttact aacaaaacta aaaaagctat catcacactt acagctgatt ctaaagcaat
    44101 cgagttgttt gctgctgaag ctgaataatc taaggaggaa atatcgtggg aattcgtgtt
    44161 tataaaccaa caacaaacgg tcgccgtaat atgacttctt tggatttcgc tgaaatcaca
    44221 acaagcactc ctgaaaaatc attgcttgtt gcattgaaga gcaaggctgg tcgtaacaac
    44281 aacggtcgta tcacagttcg tcaccaaggt ggtggacaca aacgttttta ccgtttggtt
    44341 gacttcaaac gtaataaaga caacgttgaa gcagttgtta aaacaatcga gtacgatcca
    44401 aaccgttctg caaacatcgc tcttgtacac tacactgacg gtgtgaaagc atacatcatc
    44461 gctccaaaag gtcttgaagt aggtcaacgt atcgtttcag gtccagaagc agatatcaaa
    44521 gtcggaaacg ctcttccact tgctaacatc ccagttggta ctttgattca caacatcgag
    44581 ttgaaaccag gtcgtggtgg tgaattggta cgtgctgctg gtgcatctgc tcaagtattg
    44641 ggttctgaag gtaaatatgt tcttgttcgt cttcaatcag gtgaagttcg tatgattctt
    44701 ggaacttgcc gtgctacagt tggtgttgtc ggaaacgaac aacatggact tgtaaacctt
    44761 ggtaaagcag gacgtagccg ttggaaaggt atccgcccaa cagttcgtgg ttctgtaatg
    44821 aaccctaacg atcacccaca cggtggtggt gaaggtaaag caccagttgg tcgtaaagca
    44881 ccatctactc catggggcaa acctgctctt ggtcttaaaa ctcgtaacaa gaaagcgaaa
    44941 tctgacaaac ttatcgttcg tcgtcgcaac gagaaataat attaaactag tcgcttaagc
    45001 aactagtaaa tccgccagct cggtagcgct ccataggagt gcaagccgct gtggtacaac
    45061 atttaaagga gaaaatataa aaatgggacg cagtcttaaa aaaggacctt tcgtcgatga
    45121 gcatttgatg aaaaaagttg aagctcaagc taacgacgaa aagaaaaaag ttattaaaac
    45181 ttggtcacgt cgttcaacga tcttcccaag tttcattggt tacactattg cagtttatga
    45241 cggacgtaaa cacgtacctg tttacatcca agaagacatg gtaggccaca aacttggtga
    45301 atttgcacca actcgtactt acaaaggtca cgctgcagac gacaagaaaa cacgtagaaa
    45361 ataaggagaa cataaatggc agaaattact tcagctaaag caatggctcg tacagtacgt
    45421 gtttcacctc gtaaatcacg tcttgttctt gataacatcc gtggtaaaag cgtagccgat
    45481 gcaatcgcaa tcttgacatt cactccaaac aaagctgctg aaatcatctt gaaagttttg
    45541 aactcagctg tagctaacgc tgaaaacaac tttggtttgg ataaagctaa tttggtagta
    45601 tctgaagcat tcgcaaacga aggaccaact atgaaacgtt tccgtccacg tgcgaaaggt
    45661 tcagcttcac caatcaacaa acgtacagct cacatcactg tagctgttgc agaaaaataa
    45721 ggaggtaaaa tcgtgggtca aaaagtacat ccaattggta tgcgtgtcgg catcatccgt
    45781 gattgggatg ccaaatggta tgctgaaaaa gaatacgcgg attaccttca tgaagatctt
    45841 gcaatccgta aattcgttca aaaagaactt gctgacgcag cagtttcaac tattgaaatc
    45901 gaacgcgcag taaacaaagt taacgtttca cttcacactg ctaaaccagg tatggttatc
    45961 ggtaaaggtg gtgctaacgt tgatgcactc cgtgcaaaac ttaacaaatt gactggaaaa
    46021 caagtacaca tcaacatcat cgaaatcaaa caacctgatt tggatgctca ccttgtaggt
    46081 gaaggaattg ctcgtcaatt ggagcaacgt gttgctttcc gtcgtgcaca aaaacaagca
    46141 atccaacgtg caatgcgtgc tggagctaaa ggaatcaaaa ctcaagtatc aggtcgtttg
    46201 aacggtgcag atatcgcccg tgctgaagga tactctgaag gaactgttcc gcttcacaca
    46261 cttcgtgcag atatcgatta cgcttgggaa gaagcagata ctacatacgg taaacttggt
    46321 gttaaagtat ggatctaccg tggtgaagtt cttccagctc gtaaaaacac taaaggaggt
    46381 aaataaccaa tgttagtacc taaacgtgtt aaacaccgtc gtgagttccg tggaaaaatg
    46441 cgcggtgaag caaaaggtgg aaaagaagta gcattcggtg aatacggtct tcaagctaca
    46501 actagccact ggatcactaa ccgccaaatc gaagctgctc gtatcgccat gactcgttac
    46561 atgaaacgtg gtggtaaagt ttggattaaa atcttcccac acaaatcata cactgctaaa
    46621 gctatcggtg tgcgtatggg atctggtaaa ggggcacctg aaggttgggt agcaccagtt
    46681 aaacgtggta aagtgatgtt cgaaatcgct ggtgtatctg aagagattgc acgtgaagcg
    46741 cttcgacttg ctagccacaa attgccagtt aaatgtaaat tcgtaaaacg tgaagcagaa
    46801 taaggagaag gcatgaaact taatgaagta aaagaatttg ttaaagaact tcgtggtctt
    46861 tctcaagaag aactcgcgaa gcgcgaaaac gaattgaaaa aagaattgtt tgaacttcgt
    46921 ttccaagctg ctactggtca attggaacaa acagctcgct tgaaagaagt taaaaaacaa
    46981 atcgctcgta tcaaaacagt tcaatctgaa gcgaaataat agactaggga aggagaaatt
    47041 tcaatggaac gcaataatcg taaagttctt gttggacgtg ttgtatctga caaaatggac
    47101 aagacaatca cagttgtagt tgaaacaaaa cgtaaccacc cagtctatgg taaacgtatt
    47161 aactactcta aaaaatacaa agctcatgat gaaaacaatg ttgccaaaga aggcgatatc
    47221 gtacgtatca tggaaactcg cccgctttca gctacaaaac gtttccgtct tgtagaagtt
    47281 gttgaagaag cggtcatcat ctaatcaaac ctgaaaggag aaaactgaaa tgattcaaac
    47341 agaaactcgt ttgaaagtcg cagacaacag cggtgctcgc gaaatcttga ctatcaaagt
    47401 tcttggtggt tcaggacgta aatttgcaaa catcggtgat gttatcgtgg catctgtaaa
    47461 acaagctact cctggtggtg cggttaaaaa aggtgacgtt gttaaagcag ttatcgttcg
    47521 tactaaatca ggtgctcgtc gtgctgatgg ttcatacatc aaatttgacg aaaacgcagc
    47581 agttatcatc cgtgaagaca aaactcctcg cggaacacgt atctttggcc cagttgcacg
    47641 tgaattgcgt gaaggtggct tcatgaagat cgtgtcactt gctccagaag tactttaatt
    47701 tttaggaaca aactagtccc ctagcttcaa gctagggtgc ccttatgggc gtaagaaaaa
    47761 tcaaggagaa acctaatgtt tgtaaaaaaa ggcgacaaag ttcgcgtaat cgctggtaaa
    47821 gataagggaa cagaagctgt tgtccttact gcccttccaa aagtaaacaa agttatcgtt
    47881 gaaggtgtta acattgttaa gaaacaccaa cgtccaacta acgagcttcc tcaaggtggt
    47941 atcatcgaga aagaagcagc tatccacgta tcaaacgttc aagttttgga caaaaatggt
    48001 gtagctggtc gtgttggtta caaatttgta gacggtaaaa aagttcgcta caacaaaaaa
    48061 tcaggcgaag tgcttgatta atcacgaagg aaaggagaag tataatggca aatcgtttaa
    48121 aagaaaaata tcttaatgaa gtagttcctg ctttgacaga acaattcaac tactcatcag
    48181 tgatggctgt gcctaaagta gataagattg ttttgaacat gggtgttggt gaagctgtat
    48241 caaacgctaa aagccttgaa aaagctgctg aagaattggc acttatctca ggtcaaaaac
    48301 cacttatcac taaagctaaa aaatcaatcg ccggcttccg tcttcgtgaa ggtgttgcga
    48361 tcggtgcaaa agttaccctt cgtggtgaac gtatgtacga attcttggat aaattggtat
    48421 cagtttcact tccacgtgta cgtgacttcc acggtgttcc aacaaaatca tttgatggac
    48481 gcgggaacta cacacttggt gtgaaagaac aattaatctt cccagaaatc aacttcgatg
    48541 acgttgacaa aactcgtggt cttgacatcg ttatcgtaac aactgctaac actgacgaag
    48601 agtcacgtgc attgcttaca ggccttggaa tgccttttgc aaaataatat aggaggtaaa
    48661 tctaatggct aaaaaatcaa tggtagctag agaggctaaa cgccaaaaaa ttgttgaccg
    48721 ttatgctgaa aaacgtgctg cattaaaggc ggcaggggac tacgaaggtt tatctaaatt
    48781 acctcgcaac gcttcaccga ctcgtttaca taatcgttgt agagttacgg ggcgcccaca
    48841 ttcagtttac cgcaaatttg gtctgagtcg tatcgctttt cgcgaacttg cgcataaagg
    48901 tcaaattcct ggtgtaacaa aagcatcttg gtaatttaag atatcaaggg cgtcaaaact
    48961 ccaagtaaaa ataggaaact tgacgaagaa actaaagttt ctaggaaagt ttatcttttt
    49021 cacacagagt ttagcccggg ttcaattggg cttgccaatt tgaacacgag ctacagcttt
    49081 ggcaaaaaag accaatttgc tttggagcat tgcttctgca ttaaattgtc tatttttgct
    49141 cgtgctgtta cgctctttgt atcatgtatt aactagcaag tgcaacttgc aaactactag
    49201 taagaggaga aaaacaaaat ggttatgact gacccaatcg cagacttcct aactcgtatt
    49261 cgtaatgcta accaagctaa acacgaagta cttgaagtac ctgcatcaaa catcaaaaaa
    49321 gggattgctg aaatccttaa acgcgaaggt tttgtaaaaa acgttgaaat catcgaagat
    49381 gacaaacaag gcgtcatccg tgtatttctt aaatacggac caaatggtga aaaagttatc
    49441 actaacttga aacgtgtttc taaaccagga cttcgtgtct acaaaaaacg tgaagacctt
    49501 ccaaaagttc ttaacggact tggaattgcc atcctttcaa cttctgaagg tttgcttact
    49561 gataaagaag cacgccaaaa gaatgttggt ggtgaggtta tcgcttacgt ttggtaaaat
    49621 caagatacaa agctcgtaaa gaacaaagca aaattaggaa gttggagaag tttgtttaca
    49681 aacaagccaa cttatctatt ttgcacagtt cttagagcgt gttcagttca gctcttgaac
    49741 taaataagta tctgaacccc gtgaaaactg gccgttttgg cctgacaatt taacaggaga
    49801 aaataaacat gtcacgtatt ggtaataaag ttatcgtgtt gcctgctggt gttgaactcg
    49861 ctaacaatga caacgttgta actgtaaaag gacctaaagg agaacttact cgtgagttct
    49921 caaaagatat tgaaatccgt gtggaaggta ctgaagtaac tcttcaccgt ccaaacgatt
    49981 caaaagaaat gaaaactatc cacggaacta ctcgtgccct tttgaacaac atggttgttg
    50041 gtgtatcaga aggattcaag aaagaacttg aaatgcgtgg ggttggttac cgtgcacagc
    50101 ttcaaggatc taaacttgtt ttggctgttg gtaaatctca tccagacgaa gttgaagctc
    50161 cagaaggaat tacttttgaa cttccaaacc caacaacaat cgttgttagc ggaatttcaa
    50221 aagaagtagt tggtcaaaca gctgcttacg tacgtagcct tcgttcacca gaaccatata
    50281 aaggtaaagg tatccgttac gttggtgaat tcgttcgccg taaagaaggt aaaacaggta
    50341 aataatgttg agtggttgat catcaaccac caacctattt tccaactttg tgcatagcac
    50401 acgatttaaa actaaagagg tgaaaactgt gatttcaaaa ccagataaaa acaaactccg
    50461 ccaaaaacgc caccgtcgcg ttcgcggaaa actctctgga actgctgatc gcccacgttt
    50521 gaacgtattc cgttctaata caggcatcta cgctcaagtg attgatgacg tagcgggtgt
    50581 aacgctcgca agtgcttcaa ctcttgataa agaagtttca aaaggaacta aaactgaaca
    50641 agccgttgct gtcggtaaac tcgttgcaga acgtgcaaac gctaaaggta tttcagaagt
    50701 ggtgttcgac cgcggtggat atctatatca cggacgtgtg aaagctttgg ctgatgcagc
    50761 tcgtgaaaac ggattgaaat tctaatagga ggacactaga aaatggcatt taaagacaat
    50821 gcagttgaat tagaagaacg cgtagttgct gtcaaccgtg ttacaaaagt tgttaaaggt
    50881 ggacgtcgtc ttcgtttcgc agctcttgtt gttgttggtg accacaatgg tcgcgtagga
    50941 tttggtactg gtaaagctca agaagttcca gaagcaatcc gtaaagcagt agatgatgct
    51001 aagaaaaact tgatcgaagt tcctatggtt ggaacaacaa tcccacacga agttctttca
    51061 gaattcggtg gagctaaagt attgttgaaa cctgctgtag aaggttctgg agttgccgct
    51121 ggtggtgcag ttcgtgccgt tgtggaattg gcaggtgtgg cagatattac atctaaatca
    51181 cttggttcta acactccaat caacattgtt cgtgcaactg ttgaaggttt gaaacaattg
    51241 aaacgcgctg aagaaattgc tgcccttcgt ggtatttcag tttctgattt ggcataagaa
    51301 aggggataaa atggctcaaa ttaaaattac tttgactaag tctccaatcg gacgcattcc
    51361 atcacaacgt aaaactgttg tagcacttgg acttggcaaa ttgaacagct ctgttattaa
    51421 agaagataac gctgctatcc gtggtatgat tacagcagta tctcacttag taacagttga
    51481 agaagtaaac taatgaagtt ttaggggatg tgcactgtac catcccctaa aactagatat
    51541 agtcatctat gatgacatcg tataggcgag ttgatggggg agacaacctt ttctccctta
    51601 tcggcgctag cattttacaa aagaggagaa aataaaaatg aaacttcatg aattgaaacc
    51661 tgcagaaggt tctcgtaaag tacgtaaccg cgttggtcgt ggtacttcat caggtaacgg
    51721 taaaacatct ggtcgtggtc aaaaaggtca aaaagctcgt agcggtggcg gagttcgcct
    51781 tggttttgaa ggtggacaaa ctccattgtt ccgtcgtctt ccaaaacgtg gattcactaa
    51841 catcaacgct aaagaatacg caattgtgaa ccttgaccaa ttgaacgtct ttgaagatgg
    51901 tgctgaagta actccagttg ttcttatcga agcaggaatt gttaaagctg aaaagtcagg
    51961 tattaaaatt cttggtaacg gtgagttgac taagaaattg actgtgaaag cagctaaatt
    52021 ctctaaatca gctgaagaag ctatcactgc taaaggtggt tcagtagaag tcatctaaga
    52081 gaggtgacct atgtttttta aattattaag agaagctctt aaagtcaagc aggttcgatc
    52141 aaaaatttta tttacaattt ttatcgtttt ggtctttcgt atcggaacta gcattacagt
    52201 tcctggtgtg aatgccaata gcttgaatgc tttaagtgga ttatccttct taaacatgtt
    52261 gagcttggtg tcggggaatg ccctaaaaaa cttttcgatt tttgccctag gagttagtcc
    52321 ctatatcacc gcttctattg ttgtccaact cttgcaaatg gatattttac ccaagtttgt
    52381 agagtggggt aaacaagggg aagtaggtcg aagaaaattg aatcaagcta ctcgttatat
    52441 tgctctagtt ctcgcttttg tgcaatctat cgggattaca gctggtttta ataccttggc
    52501 tggagctcaa ttgattaaaa ctgctttaac tccacaagtt tttctgacga ttggtatcat
    52561 cttaacagct ggtagtatga ttgtcacttg gttgggtgag caaattacag ataagggata
    52621 cggaaacggt gtttccatga ttatctttgc cgggattgtt tcctcaattc cagagatgat
    52681 tcagggcatc tatgtggact actttgtgaa cgtcccaagt agccgtatca cttcatctat
    52741 cattttcgta atcattttga ttattactgt attgttgatt atttacttta caacttatgt
    52801 tcaacaagca gaatacaaaa ttccaatcca atatactaag gttgcacaag gtgctccatc
    52861 tagctcttac cttccgttaa aggtaaaccc tgctggagtt atccctgtta tctttgccag
    52921 ttcgattact gcagcgcctg cggctattct tcagtttttg agtgccacag gtcatgattg
    52981 ggcttgggta agggtagcac aagagatgtt ggcaactact tctccaactg gtattgccat
    53041 gtatgctttg ttgattattc tctttacatt cttctatacg tttgtacaga ttaatcctga
    53101 aaaagcagca gagagcctac aaaagagtgg tgcctatatc catggagttc gtcctggtaa
    53161 aggtacagaa gaatatatgt ctaaacttct tcgtcgtctt gcaactgttg gttccctctt
    53221 ccttggtgtg atttccattt taccgattgc agctaaagat gtatttggtc tttctgatgt
    53281 tgttgccttt ggtggaacaa gtctcttgat cattatctct acaggtatcg aaggaatcaa
    53341 gcaattggaa ggttacctat tgaaacgtaa gtatgttggt ttcatggaca gaacagaata
    53401 aaagtattta ctgaatcagt aaatactgag ggagtggagg tttaaactct gacatttgta
    53461 agagttggat ctcccctctt ctattttgtt tttaaatagg ggtgaaaaga ctttttgctt
    53521 ctatttaaaa ataaaataag gagatcaaat catgaatctt ttgattatgg gcttacctgg
    53581 tgcaggtaag ggaactcaag cagcaaaaat cgtagaacaa ttccatgttg cacatatctc
    53641 aacaggtgat atgttccgcg ctgcaatggc aaatcaaact gaaatgggtg ttcttgctaa
    53701 gtcatatatt gacaagggtg aattggttcc tgacgaagtt acaaatggaa tcgtaaaaga
    53761 acgcctttca caagatgata ttaaagaaac aggattctta ttggatggtt acccacgtac
    53821 aattgaacaa gctcatgcct tggacaaaac attggctgaa cttggcattg aactagaagg
    53881 tattatcaat attgaagtga accctgacag cctcttggaa cgtttgagtg gccgtatcat
    53941 ccaccgcgta actggagaaa ctttccacaa ggtctttaac ccaccagttg actataaaga
    54001 agaagattac taccaacgtg aagatgataa gcctgagaca gtaaaacgtc gtttggatgt
    54061 taatattgct caaggagaac caatcattgc tcactaccgt gccaaaggtt tggttcatga
    54121 catcgaaggt aatcaagata tcaatgatgt cttctcagat attgaaaaag tattgacaaa
    54181 tttgaaataa agcgtttttc gcacttgcaa aaatccgcta caaatgttat actgagatag
    54241 tctgacttat aattgttgtc tctgtgtcta gaggcatcga atcgaaattt atggaggtgc
    54301 ttttgcgtgg caaaagacga tgtgattgaa gttgaaggca aagtagttga tacaatgccg
    54361 aatgcaatgt ttacggttga acttgaaaat ggacatcaga ttttagcaac agtttctggt
    54421 aaaattcgta aaaactatat tcgtatttta gcgggagatc gtgttactgt cgaaatgagt
    54481 ccatatgact tgacacgtgg acgtatcact taccgcttta aataatcgaa aaacttggag
    54541 ggataagaaa tgaaagtaag accatcggtc aaaccaattt gcgaatactg taaagttatt
    54601 cgtcgtaatg gtcgtgttat ggtaatttgc ccagcaaatc caaaacacaa acaacgtcaa
    54661 ggataagata gaaaggagaa aacatggctc gtattgctgg agttgatatt ccaaatgaca
    54721 aacgcgtagt aatctcattg acttatgttt atggtatcgg acttgcaaca tctaagaaaa
    54781 ttttggctgc tgctggaatc tcagaagatg ttcgtgtacg tgatcttaca tcagatcaag
    54841 aagatgctat ccgtcgtgaa gtggatgcaa tcaaagttga aggtgacctt cgtcgtgaag
    54901 taaacttgaa catcaaacgt ttgatggaaa tcggttcata ccgtggtatc cgtcaccgtc
    54961 gtggacttcc tgtccgtgga caaaacacta aaaacaacgc tcgcactcgt aaaggtaaag
    55021 ctgttgcgat tgctggtaag aaaaaataat ataggaggta aaagtcttgg ctaaaccaac
    55081 acgtaaacgt cgtgtgaaaa agaatatcga atctggtatt gctcatattc acgctacatt
    55141 taataacact attgttatga ttactgatgt gcatggtaat gcaattgctt ggtcatcagc
    55201 tggtgctctt ggtttcaaag gttctcgtaa atctacacca ttcgctgctc aaatggcttc
    55261 tgaagctgct gctaaatctg cacaagaaca cggtcttaaa tcagttgaag ttactgtaaa
    55321 aggtccaggt tctggtcgtg agtcagctat tcgtgcgctt gctgccgctg gtcttgaagt
    55381 aacagcaatt cgtgatgtga ctccagtgcc acacaatggt gctcgtcctc caaaacgtcg
    55441 ccgtgtataa tcatcgcatt acactgcttt tcgtttaaga gggagtaact aaatgatcga
    55501 gtttgaaaaa ccaaatataa caaaaattga tgaaaataaa gattatggca agtttgtaat
    55561 cgaaccactt gaacgtggct acggtacaac tcttggtaac tctcttcgtc gtgtacttct
    55621 agcttctcta ccaggagcag ctgtgacatc tatcaacatt gatggtgtgt tacatgagtt
    55681 tgacacagtt ccaggtgttc gtgaagacgt gatgcaaatc attctgaaca ttaaaggaat
    55741 tgcagtgaaa tcgtacgttg aagacgaaaa aatcatcgaa ctggatgttg aaggtcctgc
    55801 tgaagtaaca gctggtgaca ttttgacaga tagcgatatt gaaattgtaa atccagatca
    55861 ttatctcttt acaatcggtg aaggttcttc tctaaaagcg actatgactg ttaacagtgg
    55921 tcgtggatat gtacctgctg atgaaaataa aaaggataat gcaccagttg gaacacttgc
    55981 tgtagattct atttatacac cagttacaaa agtcaactat caagtggaac ctgctcgtgt
    56041 aggtagcaat gatggtttcg acaaattaac ccttgaaatc ttgacaaatg gaacaattat
    56101 tccagaagat gctttagggc tttcagcacg tattttgaca gaacatcttg atttgtttac
    56161 aaatcttact gagattgcta agtcaactga agtgatgaaa gaagctgata ctgaatctga
    56221 cgaccgtatt ttagatcgta cgattgagga actggacttg tctgtgcgtt catacaactg
    56281 tttaaaacgt gccggtatca atactgtgca tgatttgaca gaaaaatctg aagcagagat
    56341 gatgaaagta cgaaatcttg gacgcaagag tttggaagaa gtgaaactca aactcattga
    56401 tttgggtctt ggattaaaag ataaataaag gaggaataca tggcttaccg taaactagga
    56461 cgcactagct cacaacgtaa agcaatgctt cgcgatttga caactgacct tttgatcaac
    56521 gaatcaatcg tgacaactga agctcgtgct aaagaaatcc gtaaaactgt tgaaaaaatg
    56581 attactctag gtaaacgtgg tgatttgcat gcacgtcgtc aagcagctgc tttcgtacgt
    56641 aatgaaatcg catctgaaaa ctatgatgaa gcaactgata agtacacttc tactacagca
    56701 cttcaaaaat tgttctcaga aatcgcacct cgttatgctg aacgtaacgg tggatacact
    56761 cgtatcctta aaactgaacc acgtcgtggt gatgcagcgc caatggcgat catcgaatta
    56821 gtataaaatc atcaattttg ttgagtgtta tgatgatgga gtcttgtgct cttagtctag
    56881 ctctggtcta ccgctaggat ttcggtccta gcgggaacac tcatcataag ttgggatagt
    56941 agacgcttgt ttacgaaatt gtttttttct taagaacaac ttcgtaagca ggcgtttttg
    57001 agtattttcg ttagaattat gctatactat ttgaaaagaa tcctgtttaa tgttaaggtt
    57061 tcttatttta agaagaattg gagtttactt atgaaagcca ttataactgt tgttggtaaa
    57121 gataaatctg gaattgttgc aggtgtttct ggtaaaattg cagaattagg attgaatatt
    57181 gacgatatct ctcaaactgt cttggatgaa tattttacga tgatggctgt tgtatctagt
    57241 gatgaaaagc aagattttac ctatcttcgt aatgaatttg aagcttttgg gcaaactttg
    57301 aatgtaaaaa tcaatattca gagtgcagcg attttcgaag ctatgtataa tatctaggag
    57361 gttatcatgg atattagaca agttactgaa accatcgcca tgattgagga gcaaaatttc
    57421 gatatcagaa ccattaccat ggggatttct cttttggatt gtatcgatcc agatatcaat
    57481 cgtgctgcgg agaaaatcta tcaaaaaatt acgacaaagg cggctaattt agtagctgtt
    57541 ggtgatgaaa ttgcggctga gttgggaatt cctatcgtta ataagcgtgt atcggtgaca
    57601 cctatttctc tgattggggc agcgacagat gagacggact acgtggttct ggcaaaagcg
    57661 cttgataagg ctgcgaaaga gattggtgtg gactttattg gtggtttttc tgccttagta
    57721 caaaaaggtt atcaaaaggg agatgagatt ctcatcaatt ccattcctcg cgctttgact
    57781 gagacggata aggtctgctc gtcagtcaat atcggctcaa ccaagtctgg tattaatatg
    57841 acggctgtgg cagatatggg acgaattatc aaggaaacgg caaatctttc agatatggga
    57901 gcggccaagt tggttgtatt cgctaatgct gttgaggaca atccatttat ggcgggtgcc
    57961 tttcatggtg ttggggaagc agatgttatc atcaatgtcg gagtttctgg tcctggtgtg
    58021 gtgaaacgtg ctttggaaaa agttcgtgga cagagctttg atgtagtagc cgaaacagtt
    58081 aagaaaactg cctttaaaat cactcgtatc ggtcaattgg ttggtcaaat ggccagtgag
    58141 agactgggtg tggagtttgg tattgtggac ttgagtttgg caccaacccc tgcggttgga
    58201 gactctgtgg cacgtgtcct tgaggaaatg gggctagaaa cagttggcac gcatggaacg
    58261 acagctgcct tggccctctt gaacgaccaa gttaaaaagg gtggagtgat ggcctgtaat
    58321 caggtcggtg gtctatctgg tgcctttatc cctgtttctg aggatgaagg aatgattgct
    58381 gcagtgcaaa atggctctct taatttagaa aaactagaag ctatgacggc tatctgttct
    58441 gttggattgg atatgattgc catcccagaa gatacgcctg ctgaaactat tgcggctatg
    58501 attgcggatg aagcagcaat cggtgttatc aacatgaaaa caacagctgt tcgtatcatt
    58561 cccaaaggaa gagaaggcga tatgattgag tttggtggtc tattaggaac tgcacccgtt
    58621 atgaaggtta atggggcttc gtctgtcgac ttcatctctc gcggtggaca aatcccagca
    58681 ccaattcata gttttaaaaa ttaagaaaat aggagaaatt ttaagttcta tttaagatta
    58741 gatgtgtata ctataatcat taaataaaga cctcctaata ttatttgaaa cagataacac
    58801 tgaattagtt tgaatttgat tttcatctaa tatctttatt taatgaactc ctaaactttt
    58861 tcataataat ctccttcaaa agtcgcctgt atgggtggct tttattttat cattcatgat
    58921 ataatagaag caaacggagg acggaaaatg gtaaaagtac gattgtattt gatacgtcat
    58981 ggcaagacca tgtttaacac gattggtcgc gcgcaaggtt ggagcgatac tcccttaact
    59041 gctgaaggtg aacgagggat tcaagagtta ggaattggtt tgcgagaatc tgatctacag
    59101 tttgagcgcg cttattcgag tgattctggt cgtaccattc agaccatggg aattatcctt
    59161 gaagaacttg gcttgcaggg gaaaatccct tatcgcatgg acaagcgtat cagagaatgg
    59221 tgtttcggta gttttgatgg agcctatgat ggcgatcttt tcatgggcat tattcctcgt
    59281 atctttaatg tggaccacgt tcaccaattg tcttatgctg aactggctga gggcttggta
    59341 gaggtcgata cagctggttg ggctgaaggc tgggaaaaac tcagtggccg aatcaaggaa
    59401 ggctttgaaa tgattgcaaa agaaatggaa gatcaaggtg gaggtaacgc ccttgttgtc
    59461 agccatggaa tgactattgg aaccattgtt tatctgatta atggcatgca tccgcatggt
    59521 ctagataatg gtagcgtgac gattcttgaa tatgaggacg gtcagtttag ggttgaagtt
    59581 gtcggtgacc gtagttaccg agagctagga cgtgagaaga tggaagaagg ctctatttaa
    59641 tcagtctaga cttgcttgcc atgagctagg gatttgataa gaatatcaag ataagaaaaa
    59701 acagccgagg gtactccttt cggctgtttt tgatgtggaa aactaaagtg taatgctatt
    59761 gcttttagag attttcataa acaagagcaa ggaacctact gttagaacag tcaggatagt
    59821 tgacaaggct gcggctacac cgtaatttcc tctgagaacc tctgtataaa tagctacagt
    59881 cattgttctt gttttgacat tgtagaggag gatagaagta gagagttctg aaatcagtgt
    59941 gacccaagat aggatggctc cagaaataat atccgatagc atcattggag ttgtaatctt
    60001 agcaaagata ttgagacgac tacttcctaa gctttcagca gcttcttcaa tacttggtgc
    60061 tatttgttgt aagctagcaa cagatgagcg aatagtataa ggtaattttc tggcagatag
    60121 agacataatc aagatgaaag cagtccctgt aatcataaga aatccacttc caaatagacc
    60181 agagaaatga aggcaatccc tagaacggtt cctggtacaa tataaggtac catactgagg
    60241 ctgtcaatta agtttctaaa caaattccgt tttctaacgg ctaggtagga gataaatgtc
    60301 gcaaatagaa caactagaac taaggcaatc aaagggatac gaatggtatt gaaaatagca
    60361 gatcccatac gatggaaagc taccttgtaa ctgtttggag aataaccttt aacagatacc
    60421 atacctgatg tttttaggaa agaggtataa attaagtaga tttgaggtaa aacagagata
    60481 aagataattc cgtagactgt tgcataaatg gcagctattt ttccttttgt agttttttta
    60541 ggctcaattg gatggagcag attcatgctg aaactgtagc ggtttgcaat gtgtttttgg
    60601 ataaggaaaa ttgccaaggc aatgataatc gccataattg caaaagcaga atttcctcca
    60661 acctcgctaa taaattgggt ataaatcagg acagggaaag tccgatatcc ttcgccaatc
    60721 aacataggcg ttccaaagtc tgagaatgct ctcataaata caagcaagga gctgctagta
    60781 aggttggaac taggagaggt aaaacaaccg ttacgatacg cttaaatccg aaggaccccc
    60841 atgctttcag ctgcttcaag tagagaattg tcaatactgt tcattgttcc agcaacatat
    60901 agaaatacca gtgggaatag ttgcagtgta aagacaagta caattccttt gaatccataa
    60961 atatcgatag ctggaagatg aagggcattt gtcaaaaatt tagtgatgac cccatttcgt
    61021 cctagcaaga gaacccagga gtaggctcct acgaaaggag ctgacatgga agcaatgata
    61081 atcaatattt gtagaaattt ctcccccttg aagtcataca tagagaagag ataagctaat
    61141 agggttccta caactaagga agtgacagta gcggtaatgg aaaccttgaa actgttgact
    61201 agtgtctcag agtagtagac tttactaaag aaagtgacaa aattagctag tgagaattat
    61261 ccttcatgta taagtgctta cttgagcacg gtaatgatag gataaacgag aaagacaaga
    61321 taggtaagaa agatgaagaa agaggaggct gtccaaatat ttagtttttt acattccatg
    61381 gttgactcct tttatcaggt tttgggaacc atctgcagaa aagatgttta atttttgcgt
    61441 attgattcgt agacgaatac gattgtcttt ttgtagatct tcttcaaaag ttgattcttc
    61501 actaacttga atttttgagg caaaacctgt ctcaatgaaa tactccgtat ttagtccaag
    61561 atagacgcta tcgctaatag ttccttcaat atctccagat tcatctttga taaactcttc
    61621 gggacgaatg cttacatgaa tagcttgctc ctcaacctga tcaagagctg gcattcgaag
    61681 ggcatagcca tctgaaaaga cgatataagc gccgtcgctc cgtttttcaa gattggcagg
    61741 gataatattt gtgcgtccga taaaggttgc cacaaactca ttagctggtt tatgatagag
    61801 ttcttttggt cggccgattt gttggatcac cccatctttc ataacagcaa tttgatctga
    61861 aatagccatg gcttcttctt ggtcgtgggt tacataaaca gttgtaattc ccacttcgtg
    61921 ttggatttct cggatggctt gacgcatatc caagcgaagt ttggcctcca gattactaag
    61981 tggctcgtcc atgaggagaa cacttggatt aaccgctaag gcgcatgcca aggtgacacg
    62041 ttgttgttgt ccaccactga gtttatcggg ctttcgatcc gcatattgag caatttgcat
    62101 gagttcaaga tacttgttgg tctgttgaat caattcttct tttggaacct tcttttgctt
    62161 aagaccaaaa gcaacgttgt ctcggacagt caaatgtggg aaaatagcgt agttttggaa
    62221 aaccatcccg atattgcgtt tgctgggttc catattattg atttttgtat catcgaagta
    62281 aaattctcca tctttgatac tgttgaaacc tgcaatcata cgaagaaggg tcgttttccc
    62341 acatcctgaa ggtccaagaa gggtaaagag acttcctttt ggaattgtaa tgttcaaatt
    62401 ctcaataaca gggacatcgt ggtagatttt ttggcgttaa taattttgat ctcactcata
    62461 gtgaaccttt tttactgttt agattggata tctgtaaaga cttcgttgta tttcttaacg
    62521 atatctgatt tattcttgat gacataatca taatcttcag tgagtgtttt gattttgtca
    62581 attggtttca tgttttcgct tgttttagca tttttacgaa caggacggtt agtagtggtt
    62641 gtaccaagtg tatcttgtac ttcttgagag ataataaaat cgataaattt cttgtcattt
    62701 tccatatttt tagctttttt aacgatagca gcactagcag gtaggaagac ggttccttct
    62761 tttggataga ctactttaat gttagctccg tcatttaaga gtttaactgc tggatcctca
    62821 taagagagac caacagccat ttctccatca acgactgctt tatagacacc agatgaactt
    62881 gaaccgattt tatagtgtat tgaaactaga atagtacacc tctacttcta aaacattgtt
    62941 agaaatcgat ttgactgtcc tgattaattt gtcctgttct tatttcattt cactatgcca
    63001 tcaataagtg tgaaaagatc ttttacataa gaccaagcct tatcatcttt gtaaccacct
    63061 tgagcttgta gcatatttgt taattgagca aaggcgctag aagagtttgc tgggtcagca
    63121 gttgcgattt ttccttttag ttcaggtttg agaaggtcgc tatatccttc gatgttcatg
    63181 cctttagtta aatcagggtt gacgattaaa acactaccat ctagtgtata aggagtagag
    63241 aagccagttg tgttttgata ttctttgata acattatcat tttcttttga aatatagttt
    63301 tcaaagagtt ctccgtgggt agcatattgt gtataagaac caccaaagat aacatcagct
    63361 acaggaactt ctttttctga ctctagtttt ttgaaaagtt ctccagtacc agcttgaatc
    63421 agttctattt tgataccata tttttcttca aaggcaggaa tagttgctcc aattaagcct
    63481 tctgagtttg gtgaataaac gactagcgaa ccaccgtctc ctttatcaga tgaactgtca
    63541 tcggcagatt cattagaaga acaagcagca taatacatct atttcttttt catgatggat
    63601 atctccgttg tgttatttaa gtttatttta aaacaatgta agcgttttta aaacctacaa
    63661 ttctattcta tagtgtattg aatctataac agtacacttt gactgctaaa atatttctat
    63721 aaattaattt gactttcctg atagagatgt tcacatctta tttcaattca ctatattaga
    63781 gtaaaattct ctacaaaaag aagaatagcc tatttgacta ttcttccgag tgatttcaat
    63841 ttctttgggg aaatatggag atacttttta aatactttgg caaaatattt atagtctgaa
    63901 aatccaactt ggtcagagat agcgcttaaa ggggagttgg gagattcagc catcttatct
    63961 atagcttgtt tcaggcgata ttgtaagata tattcattta gagtcatggg gaagtcttct
    64021 ttgataacct tatatagatg cttgttatct gcaaatagct agagcatttc aaggaaataa
    64081 actagaaata acaattaata tggtggttca gaaatacctt ttgataatca ataagatctt
    64141 caggatgaag tgcgttgatt ccatccattt catatttccg atttaataaa cgatatttgt
    64201 tttcaccaaa ttgatgaaaa gggagtagtt gaacttggtc gatatttaat gagttaaata
    64261 gagtagcgaa tttttctgca tcctctaaac tattgttaaa attaggaata actgggattc
    64321 ttaaaacgat agttttattt tgagaaaaag cataatgaat gtttttaata atcatttgat
    64381 taaaaacccc agtcactttt ttatgtttta tagaattata atgttttagg tctgtgtaga
    64441 taaaatccac atattgaatt aaatcaataa atttttcatg atcaacaaag gcagtagttt
    64501 caatggcagt gtgtatgtga tgttctttag ctgattttaa gatggcttta gcaaattcaa
    64561 actgagcaaa tatttcacct cctgataaag ttaaacctcc accggattct tcgtaaaatt
    64621 ctttgtcttt taatacctct gtaataattt cttctacagt cttttcttca ccgactaagg
    64681 tgaatttctc tcgttgagca tctttcattt tttcaggttt cattctttga gattcaggat
    64741 tagaacacca tggacagcgc agaggacatc cttttaaaaa aacagttgta cgaatacccg
    64801 ggccgtcatg aattgaaaag tgttgaatat taaaaataat tcctttagat atttccataa
    64861 gagttcctcc tcttcataag tctatcctat cacaaaaacg aaagaaaaac aattacgaac
    64921 gaaagtttaa tttgttgttt tttgcttttt attttggtag aataaaacgg aggtgtaaaa
    64981 tgaaaagatt ggaacaaatt attaaattag tatcagaata tgaaaagatc gacgttaata
    65041 cattatcgga aaaattaaat gtatcgaaag taacgattag aaaagattta gataaattag
    65101 agtcaaaagg tttattacac agagagcatg gatatgctgt attaaatagt ggagatgact
    65161 taaatgtacg tttgtcaatt aactatgaaa ttaagagaaa aattgttcag gaagcagtaa
    65221 aattggtgtc agataatgaa acaataatga tagaatctgg atcgacctgt gctttacttg
    65281 ctgaggaaat ttgcaagcaa aaaagaaatg ttacgattgt aacaaattca ttttttatag
    65341 caaattttgt gagagcttat gattcatgtc gtattattgt tcttggtgga gagtttcaga
    65401 aggattcaca ggttactgtg ggacctttat taaaagaaat gatacagact tttcatgtgc
    65461 atcaagcttt tgttgggaca gatggttacg ataaagagat gggctttaca ggaaaagatt
    65521 taatgcgcag tgaggtagtt caatatattt cagcagcgtc ggataaagtc attgtactaa
    65581 ctgactcaag taaatttgat aaaaggggta cagtaagaag atttgcttta agtcaagtct
    65641 atgaagtaat aacagacgaa aaactttcta aacaaaatat agctacatta gaaaatgctg
    65701 ggataatggt taaggtagtt tcgtaagagg ttaaatgtat gaatcaagat aggaataaac
    65761 tgctctctaa aattgcttat ctgtattata ttgaaaactt aaatcagtca caaatagcag
    65821 caaaattagg aatttataga acctctatta gtagaatgtt aacagaagca aggaatgcag
    65881 gaattgttaa aattgaaata gagaattttg ataccaatat gtttaagttg gaaaattatg
    65941 taaaagaaaa atatggtctg gaaagtttag aaattattcc aaatgaattt gatgataatc
    66001 caacaatttt atctgaaaga atttctcaag ttgcagcagg tgtccttagg aatctaattg
    66061 atgataatat gaaaattggc ttttcttggg ggaaaagttt aagtaattta gtagatttaa
    66121 ttcacagtaa aagtgtccga aatgttcact tctatcctct agcaggtggt cctagtcaca
    66181 tacacgctaa ataccatgtg aatacactga tttatgaaat gtctagaaaa tttcatggag
    66241 agtgtacatt tatgaatgca acgattgtgc aagaaaataa attgttagca gatggtattt
    66301 tgcaatcaag atattttgaa aatttgaaaa atagttggaa agatttagat atagctgtag
    66361 tcggaattgg tgattttagc aataaaggaa agcatcaatg gttagacatg cttacagagg
    66421 atgactttaa agaattgacc aaagttaaaa ctgtaggaga aatttgttgt cgattttttg
    66481 attcaaaagg taaagaagtc tatgaaaact tacaggaaag aacgatagca atctctttgg
    66541 aagatttaaa aaatattcct caaagtttag ctgttgctta cggtgatacg aaagtatctt
    66601 cgattctttc tgtcttgcgt gctaatttag taaatcattt gattacagac aaaaatacaa
    66661 ttttaaaagt tttggaagaa gatggggatt tgacttttag agagattcta ggtgagtgaa
    66721 aatgatagac tgatcagttt atcgtttttt ctttttagtc gattgcacat ttgtgctcat
    66781 ataaacaaaa atagtttatc tgttgttttt ggattgacaa ctttattatg tagttgtatt
    66841 ctatagttac aaaagaaaat tttaaaattt caaatgaaaa aagcttttta catagtgaaa
    66901 tgaggaggaa tttatggaaa ttattgttcc agatcaaatt atcatgggtt taattttata
    66961 tgctggtgat gcgaaacaac atatttataa agcgttagat tacataaaaa atggtacatg
    67021 tgaacggtgt gaagaagaaa tccagttagc tgatgccgcc ttattagaag cccataatct
    67081 acaaacaaaa ttcttggcac aagaagcgtc tggtacaaag acagaaatta cagctctctt
    67141 tgttcattca caagatcatc tcatgactag tatgacggag attaatttaa tcaaagaaat
    67201 tattagtttg agaaaagaac ttcataaaaa ataatactag agtattatca ttgttattaa
    67261 catagaggag gaaaacataa tggtgaagat tggtttgttt tgtgcagcag gtttttctac
    67321 tggtatgctt gtaaataata tgaaaattgc agcgcaagct agtggagttg aggcagaaat
    67381 agaggcgttt tctcagtcta aattagcgga ttatgcgcca aatatagatg ttgcactatt
    67441 gggtccacaa gttgcttata cattagataa atcaaaagaa atttgtgata agtgtgatgt
    67501 tccgatagct gttattccga tgatggacta tggtatgtta gatggaaaaa aagtattaga
    67561 tttggcccta tctttgatta gtgggtaaga aaaggagatt tattatggca aagatggatg
    67621 ttcagaaaat cattgcaccg atgatgaagt ttgtgaatat gcgtggcatt atagctctaa
    67681 aagatgggat gttagcaatt ttgccattga cagtagttgg tagtttattc ttgattatgg
    67741 gacaattgcc gttcgaagga ttaaataaga gcattgctag tgtttttgga gctaattgga
    67801 cagagccgtt tatgcaagta tattcaggaa cttttgctat tatgggtcta atttcttgtt
    67861 tttcaattgc ctattcttat gctaagaata gcggagtaga ggctttacca gctggagttc
    67921 tatctgtatc tgcattcttt attttgctaa gatcatctta tatccctaaa caaggtgagg
    67981 caattgggga tgctattagt aaagtttggt ttggaggtca aggaattatc ggtgctatca
    68041 ttataggttt ggtagtagga agtatttata ccttctttat aaagagaaaa attgttatta
    68101 agatgccaga acaagttcca caagctattg ccaaacagtt tgaagcaatg attccagcat
    68161 ttgtaatttt cttactttct atgattgtat atattttagc gaagtcattg actaatggcg
    68221 gaacattcat agaaatgatt tattctgcta ttcaagttcc attgcaaggt ttaactggat
    68281 cttggtatgg tgcaattgga attgcattct ttatatcatt tttgtggtgg tttggtgttc
    68341 atggacaatc ggtagtaaat ggagtagtga cagctctgct tttatctaat cttgatgcta
    68401 ataaagctat gttagcctct ggtaatctat cattagaaaa tggtgcacat attgttactc
    68461 aacaattttt agattcattt ttaattctat caggttcagg gattacgttt ggtcttgtag
    68521 ttgccatgct ttttgcagca aaatcaaaac aataccaagc cttaggaaaa gttgccgctt
    68581 ttccagcaat atttaacgta aatgagccag ttgtatttgg atttccgatt gtcatgaatc
    68641 cagttatgtt tgtacctttc attcttgttc ctgtacttgc agctgtgata gtatatggag
    68701 ctattgcaac aggtttcatg cagccattct caggggtaac attgccttgg agtacaccag
    68761 ctattttatc aggatttttg gtgggtggat ggcaaggagt tattactcag ctggtgatat
    68821 tagcgatgtc tacattggtt tattttccat tctttaaagt acaggatcgt ttagcttacc
    68881 aaaatgaaat caaacaatct tagaggtatt tgtgtgttac tgttaaactc acacatttgt
    68941 gctaaaaatt agagagttaa aatttttcta gttaaaagct tgaaaatttc tataaaaatc
    69001 ggtattatat tttcgaaaga aataaaaata ttttcgaaag aaaggtggtt acgatggtaa
    69061 atacagaagt agcaagaaca acaatcaaga cagaatattt tggcagcctt actgaaagga
    69121 tgaacaaata tcgagaagat gttttaaata aaaaacctta tattgatgct gagagagcag
    69181 ttctagcaac acgcgcctat gaacgataca aggaacaacc taatgtccta aaacgtgcat
    69241 atatgctgaa agaaattttg gaaaatatga ctatctatat tgaagaagaa tctatgattg
    69301 ggggaaatca agcttcttcc aataaagatg ctcctatttt tccggaatat acgctagaat
    69361 ttgttctcaa tgagttggat ctttttgaaa agcgtgatgg agatgttttc tatattacag
    69421 aagaaacaaa agaacaactt agaagtattg ctccgttttg ggaaaataat aatttacgtg
    69481 ctagagctgg tgccttatta cctgaagaag tgtctgttta tatggaaaca ggattcttcg
    69541 gtatggaagg taagatgaat tctggagatg ctcacctagc agttaactat cagaaacttt
    69601 tgcaatttgg cttaagaggt tttgaagagc gggctcgtaa agcaaaagca gctctagatt
    69661 taacagatcc agcaagtatt gataaatatc atttttacga ctctatattt atcgtaatcg
    69721 atgctattaa agtatatgca aagcgctttg ttgctcttgc taaaagttta gccgaaaatg
    69781 caaatcctaa acgtaagaaa gaattacttg agattgcaga tatttgctct agagtcccat
    69841 atgaaccggc aactactttt gcagaagcta ttcaatcagt ttggtttatt caatgtattt
    69901 tacaaattga atctaatggc cactctcttt catatggccg ttttgatcaa tatatgtatc
    69961 catatatgaa ggctgattta gaaagtggta aagaaacaga agatagcatt gttgaacgtc
    70021 tgacaaatct ttggattaag acaattacaa ttaataaggt tcgcagtcaa tcacatacat
    70081 tttcttcagc aggaagtcct ttatatcaaa atgttacaat tggtggacag actcgagata
    70141 agaaggatgc tgttaaccca ttatcttatt tggtattaaa atcagttgca caaacccatc
    70201 taccgcaacc taatctaact gtacgttacc atgcaggttt agatgctcgt ttcatgaatg
    70261 agtgtattga agtgatgaaa cttggttttg gtatgcctgc atttaataat gatgagatta
    70321 ttattccttc ttttattgca aaaggagtat tggaagatga tgcttatgat tacagtgcca
    70381 ttggatgtgt tgaaacggca gttccaggga aatggggcta tcgttgcaca ggtatgagtt
    70441 atatgaactt ccctaaggtt ctacttatca cgatgaatga tggaattgat ccggcttcgg
    70501 gtaaacggtt tgcaccaagc tttggtcatt ttaaggatat gaagaacttt tctgaattag
    70561 aaaatgcttg ggataaaaca ctaagatatt tgacacgaat gagtgttatt gttgaaaatt
    70621 ctattgattt atcattggaa cgagaagttc ctgatattct atgttcagca ttgactgatg
    70681 attgtattgg tcgtggaaaa caccttaaag aaggtggagc agtatatgat tatatatcag
    70741 gattgcaagt tggaattgca aatttgtcgg attcattagc tgcaattaaa aaattggtgt
    70801 ttgaggaaga acgtataagc ccaagtcagc tttggcatgc attggaaaca gattatgccg
    70861 gagaagaagg taaggtcatt caagaaatgt tgattcatga tgcacctaag tatggtaatg
    70921 atgatgatta tgctgacaaa ttggttactg ctgcttatga catttatgtt gatgaaattg
    70981 ctaaatatcc taatacacgt tatggaagag ggcctattgg aggaattcgt tattcaggaa
    71041 catcttctat atcagccaac gtagggcagg gacgtggaac attagcaact ccagatggac
    71101 gcaacgcggg tacaccgtta gcagagggtt gttcaccatc acataatatg gatcaacacg
    71161 gccctacatc tgttttaaaa tctgtttcaa aattaccaac agatgaaatc gtaggtgggg
    71221 ttctcttaaa tcagaaagta aatcctcaaa cgttagccaa agaagaagat aaattaaaac
    71281 taattgcttt gttacgaaca ttctttaatc gtttacatgg gtaccatatt caatacaatg
    71341 ttgtttccag agagacgttg attgacgctc agaaacatcc tgaaaaacac agagacttaa
    71401 ttgttcgtgt tgcaggatac tctgcattct tcaatgttct ttctaaggca acccaagatg
    71461 acattatagg acgtactgag catactttgt aaaataaaga ggttcttttt atggaattta
    71521 tgcttgacac attaaattta gatgagatta aaaagtggtc tgaaattttg ccgctagctg
    71581 gggtaacttc aaatcccact attgcaaaaa gagagggttc tattaatttt tttgaacgaa
    71641 tcaaagatgt aagagaattg attggctcca caccctctat tcatgttcag gtgatttctc
    71701 aagattttga aggcatctta aaggatgctc atgaaattcg aagacaagca ggagatgata
    71761 tatttatcaa agtacctgtt actccagctg gattacgtgc aataaaggtg ctaaaaaaag
    71821 agggctacca tatcactgca acagctattt atacagttat tcagggatta ttagctatcg
    71881 aagcaggagc ggattaccta gctccatatt ataatagaat ggaaaatctg aacattgatt
    71941 caaattctgt cattcgtcaa ttagttcttg ctattgatag acagaactct cctagtaaga
    72001 ttttggctgc atcctttaaa aatgtagcac aagtaaataa tgctttagct gcaggtgcgc
    72061 atgctgttac agcaggagca gatgtttttg aatcagcttt cgccatgcca tctatccaaa
    72121 aggcggttga tgatttttct gacgattggt ttgttactca aaatagtcgt tccatttaga
    72181 tagagaggaa atacatatga gaatttttgc tagtccttct agatatattc agggggaaaa
    72241 tgccttgttt gaaaatgcca aatcaatttt ggatttggga aattacccta ttctattatg
    72301 cgatcagttg gtttatgata ttgttggaaa acgatttgaa gattatctac ataggtatgg
    72361 tttccatatt gttctggcgc tatttaatgg tgaagcttct gacaatgaaa tcaatcgagt
    72421 tgttgccttg gctgagaaag aaaattgtga tagtattatc ggtcttggtg ggggaaagac
    72481 gattgatagc gcaaaagcta ttgcagattt gattgaaaag cctgttatta ttgctccaac
    72541 aattgcatcg accgacgcac ctgtatctgc tttatctgtt atttatacag atgaaggtgc
    72601 atttgatcat tatctatttt attctaaaaa tccagattta gttttggttg atacaaaagt
    72661 tatttcacaa gcccctaagc gtttattagc gtctggtatt gcagatggtt tagcaacttg
    72721 ggttgaggcg cgtgcggtta tgcaggcaaa tggaaaaact atgttgggac aacagcaaac
    72781 attggctgga gttgcaattg cgaagaaatg tgaagaaacg ctgtttgcag atggtttaca
    72841 ggctatggca gcttgtgaag ctaaagtggt gacaccagca ttagaaaata ttgttgaagc
    72901 taatacttta ttgagtggtc taggttttga aagtggagga ttagctgcgg cgcatgcaat
    72961 tcataatggt tttactgcat tgacaggtga cattcatcat ttaacacatg gtgaaaaagt
    73021 agcttatgga actttagtac aactattatt ggaaaataga cctaaagaag aacttgataa
    73081 gtatattgag ttttacaaaa aaattggtat gccaacaact ctaaaagaaa tgcatttgga
    73141 tcaagttgga tatgatgatt taataaaagt tggtaaacaa gcaactatgg agggtgagac
    73201 aattcatcag atgccgttta agatttcgcc ttcagatgtt gctcaagcta ttatcgctgt
    73261 agatgcctat gtaaattcaa aataaacaat aaggactact gttttccaaa tggtagtctt
    73321 ttattgatcc ccgtattgaa ttctatagaa gattgaaata ggatgagaac aaatcgattg
    73381 ggaaagtaaa attaatttct ataaatgttt tagcaattgt ttcgtactat ttcagattca
    73441 gtctactata cgttcttcat aaatcaaaaa gcgacatagg ttgtcggcta tttattgtga
    73501 atacattaat tagcattcca gttttatctt cggtctaaaa taagtatttt gtgctatacg
    73561 agataagcgt cttgacttac tccttgattt actgcataac aatgggataa aaagtgggag
    73621 atagagcaat tcatagtcat caaaattaat gagatacagt atacagtttt tcctttaaac
    73681 acatttcaaa ttccctcaaa aatggtataa tagtaacatc acaaaattgg agagagacca
    73741 tgagttttta caatcataaa gaaattgagc ctaagtggca gggctactgg gcagaacatc
    73801 atacatttaa gacaggaaca gatgcatcaa aacctaagtt ttatgcgctt gatatgttcc
    73861 cttatccgtc tggagctggt ctgcacgtag gacacccaga aggttatact gcaaccgata
    73921 tcctcagtcg ttacaaacgt gcgcaaggct acaatgtcct tcacccaatg ggttgggatg
    73981 cttttggttt gcctgcagag caatacgcta tggatactgg taatgaccca gcagaattta
    74041 cagcggaaaa cattgccaac ttcaaacgtc aaatcaatgc gcttggattt tcttatgact
    74101 gggatcgtga agtcaataca acagatccaa actactacaa gtggactcaa tggattttca
    74161 ccaagcttta cgaaaaaggc ttggcctatg aagctgaagt gccagtaaac tgggttgagg
    74221 aattgggaac tgccattgcc aatgaagaag tgcttcctga cggaacttct gagcgtggag
    74281 gctatccagt tgtccgcaaa ccaatgcgcc aatggatgct caaaatcacg gcttacgcag
    74341 agcgcttgct caatgactta gatgaactag attggtcaga gtctatcaag gatatgcaac
    74401 gcaactggat tggtaaatca actggtgcca atgtaacttt caaagtaaaa ggaacagaca
    74461 aggaatttac agtctttact actcgtccgg acacactttt cggtgcgact ttcactgtct
    74521 tggctcctga acatgaatta gtagacgcta tcacaagttc agagcaagca gaagctgtag
    74581 cagactataa acaccaagcc agccttaagt ctgacttggt tcgtacagac cttgctaagg
    74641 aaaaaactgg tgtttggaca ggtgcctatg ccatcaaccc tgtcaatggt aaggaaatgc
    74701 caatctggat tgcggactat gttcttgcta gttatggaac aggtgcggtt atggctgtgc
    74761 ctgcccacga ccaacgtgac tgggaatttg ccaaacaatt tgaccttcca atcgtcgaag
    74821 tacttgaagg tggaaatgtc gaagaagctg cctacacaga ggatggcctg catgtcaatt
    74881 cagacttcct agatggattg aacaaagaag acgctattgc caagattgtg gcttgcttgg
    74941 aagaaaaagg ctgtggtcag gagaaggtta cctaccgcct ccgcgactgg ctctttagcc
    75001 gtcaacgtta ctggggtgag ccaattccaa tcattcattg ggaagatgga acttcaacag
    75061 ctgttcctga aactgaattg ccgcttgtct tgcctgtaac caaggatatc cgtccttcag
    75121 gtactggtga aagtccacta gctaacttga cagattggct tgaagtgact cgtgaagatg
    75181 gtgtcaaagg tcgtcgtgaa actaacacca tgccacaatg ggctggttca agctggtact
    75241 acctccgcta tattgacccg cacaatactg agaaattggc tgatgaggac ctcctcaaac
    75301 aatggttgcc agtagatatc tacgtgggtg gtgcggaaca tgctgtactt cacttgcttt
    75361 atgctcgttt ctggcataaa ttcctctatg acctcggtgt tgttccgact aaggaaccat
    75421 tccaaaaact ctttaaccaa gggatgattt tgggaacaag ctaccgtgac caccgtggtg
    75481 ctcttgtggc aaccgacaag gttgaaaaac gtgatggttc cttctttcat atagaaacag
    75541 gggaagagtt ggagcaagcg ccagccaaga tgtctaaatc gctcaagaac gttgttaacc
    75601 cagatgatgt ggtggaacaa tacggtgccg atacccttcg tgtttatgaa atgtttatgg
    75661 gaccactcga tgcttcgatt gcttggtcag aagaaggttt ggaaggaagc cgtaagttcc
    75721 ttgaccgagt ttaccgtttg attacaagta aagaaatcct tgcggaaaac aatggtgctc
    75781 ttgacaaggc ttacaacgaa acagtcaaag ctgttactga gcaaattgag tctctcaaat
    75841 tcaacacagc tattgcccaa cttatggtct ttgtcaatgc tgctaacaag gaagataagc
    75901 tttatgttga ctatgccaaa ggctttattc aattgattgc accatttgca cctcacttgg
    75961 cagaagaact ctggcaaaca gtcgcagaaa caggtgagtc aatctcttat gtagcttggc
    76021 caacttggga cgaaagtaaa ctggttgaag acgaaatcga aatcgttgtc caaatcaaag
    76081 gtaaagtccg tgccaaactc atggtcgcta aagacctatc acgtgaagaa ttgcaagaaa
    76141 ttgctctagc ggatgaaaaa gtcaaagcag aaattgacgg taaggaaatc gtgaaagtga
    76201 ttagtgtacc aaataaattg gttaatattg ttgtaaaata accaatttat ttgctgattt
    76261 ccaacctcaa acagtttccc aaactgtttg agccaactaa actcgttaat atcgttgtga
    76321 aataaggtag gaatccttca gagtagaatc tggaggattt tttgaatctt cttatgaaag
    76381 tatggtatac tatgggcaac tataaagttt gaaaagtgaa aaaaggagaa agctatgcca
    76441 gtaaatgaat atggtcaaat gattggggag tcaatggaag gttatacacc aggtgaactg
    76501 ccttctattg atttcttaga agggcgttat gctaggatag aggctctttc agtggaaaag
    76561 catgcggagg atttgctagc tgtttatggc ccggatactc ctcgggagat gtggacctac
    76621 ctttttcagg agccagtagc agatatgggg gagctggtta gccttttaca tcagatgttg
    76681 gctcgtaagg accgttttta ctatgcaatc atagacaagg cgactggtaa ggctttggga
    76741 tctttttctc tcatgcgcat tgaccagaat aaccgagtaa tagaagtcgg agcagtcact
    76801 ttttctccag agctaagggg tacacggata ggaactgaag ctcagtatct cctagctcgc
    76861 tatgtttttg aggagctaaa ctatcgtcgc tacgagtgga aatgcgatgc cttaaatctg
    76921 ccatccagac gagctgcgga gcgtttgagc tttatttatg aaggaacctt ccgtcaggca
    76981 gtggtttata agggacgtac gagagatacg gattggttgt ctatgattga taaggactgg
    77041 cctcaagtca aagatcgttt ggaaacatgg ttgcgtcctg aaaattttga taaaaatgga
    77101 cagcagtaca agagcttgag agaactttaa gaggtgttga gatgattact attaaaaagc
    77161 aagaaattgt caagctagag gatgttttac atctctatca ggctgtcggt tggacaaatt
    77221 atacccatca accagagatg ctggagcagg ccttatctca ttcattagta atttatctgg
    77281 cacttgatgg tgatgctgtg gtgggcttga ttcgtttggt tggagatgga ttctcatcag
    77341 tattggttca ggatttaatc gttttaccaa tctatcagcg tcaaggaatt ggtagtgcct
    77401 tgatgaaaga ggctttagag gattacaaag atgcctatca agtccagctg gtgacagaag
    77461 agacagaaag aaccttgggc ttctatcgtt ctatgggctt tgaaatctta tccacctata
    77521 attgtatagg gatgacttgg atgaatcgta aaaaataaca aaacttgttt tttcttaagc
    77581 aaaatttaag gatggtctag tattatatag tcattaaata aagacctcct aactttattt
    77641 aataaaatcc taaacttttt tcatcacaat ctcctaatga agccacccaa tcaggtggct
    77701 tttttgcgtg gtgaggtgct ggtgatagaa attttttgca aaatagtaaa attttagaaa
    77761 agttaagcta gttttaagtt tcgtcttgta tcatgtagtt attaaataaa gacctcctaa
    77821 ctttatttaa taaaatccta aacttttctt tttcataata atctccctta acttcaccca
    77881 atcaggtgga gttttttagc tctatttcag gcttttgggg actattctaa aaataatttt
    77941 tcgatatttt tcggtatttt tcggattttg gtcggggaat tggcggggac ttttttagcg
    78001 aatataacta agaaataggt ctgtggtcgc ttcagctagt tcaaactcaa tttgattgta
    78061 agtgactgtt aattgaagaa ctggaactac tgtaataatg attttgcttg tctagtagtg
    78121 gcgtattaaa tgagaagtaa catgtaagat tgtctcttcg tttacgaaat caaaatttct
    78181 atgaaattga tagaatccct aacgttctct taatcttaac cgtcttgtcg tacagcggtt
    78241 gaagaaaatg taaacctttg acaaaattcg tgaactatgg gataataaaa aggaattgag
    78301 tactagttct atatcatagg ctagaaaaga ccccaagctc acgaccaaat aagcttgagg
    78361 tcttttttga cactatttgt ccttgtttag ccatttatcg actatacgaa gaacgacacc
    78421 gaccacaatt ggtccgatga tagttttgag tactaattcc atcatgggct atctcacctc
    78481 ctttcgtagg cggtgtagca gtgtcgtaga atattatacc acataagtgc taaacttagg
    78541 agtcacccaa tcaggtggct tttttgtttg tggcttggtt ttttgatata atagagccat
    78601 gagtagaatt ttagataatg agataatggg ggatgaggag ttagtagaac gcacgctccg
    78661 tcctcagtat ttacgtgaat atatcggaca ggataaggtc aaggaccagc tacaaatctt
    78721 tattgaagct gccaaaatgc gggatgaagc gctggatcat gtgctcttat ttgggcctcc
    78781 aggtctcggg aaaacgacca tggcctttgt tattgccaac gaactgggag tcaatcttaa
    78841 gcagacgtcg ggtccagtca ttgaaaaagc cggagatctg gtagctattt tgaatgagtt
    78901 agagcctggg gatgtacttt ttattgatga gatccatcgt ttgccaatgt cagtggaaga
    78961 ggtgctttat agtgctatgg aggacttcta catcgatatt atgattgggg ctggtgaggg
    79021 tagtcgtagt gttcatttgg agttaccacc ttttaccttg attggtgcga cgactcgggc
    79081 tggtatgctc tccaatccgc tacgggcacg ttttgggatt acaggccata tggagtatta
    79141 tgcccatgct ggcttgacag aaattgtcga gcggacggca gatatttttg agatggaaat
    79201 cactcatgag gcagcatctg agttggccct acgtagtcgt gggacccctc gtattgccaa
    79261 tcgtctcctc aagcgcgtgc gcgattttgc ccagataatg gggaatgggg taattgatga
    79321 tattattacc gataaggctt tgactatgct ggatgttgac catgaaggtt tggactatgt
    79381 ggatcaaaaa atccttcgta ccatgattga gatgtacagt ggaggacctg ttggtctagg
    79441 aactctttct gtgaatatcg ccgaagagcg tgagacagtt gaagatatgt atgagcctta
    79501 cttgattcaa aaaggtttta tcatgcggac acggtctgga cgggtggcga ctgctaaggc
    79561 atatgagcac ttaggttatg aatacagtga aaaataagca agaaatctta gaggctttta
    79621 gagaaaatcc agatatgatg gccattctga cgatcatccg agaccttggt ctgaaagact
    79681 cgtggttggc agcaggttct gtcagaaatt tcatttggaa tcttttgtca gacaaatccc
    79741 cttttgatca tgaaacagat atagatgtga ttttctttga tccagatttt tcttatgagg
    79801 aaaccttatt actggagaaa aagctgcgag aggattttcc tcagtaccag tgggaattga
    79861 aaaatcaggt ctatatgcat cagcacagtc ctcacactgc ttcctatacc agttctcgtg
    79921 atgctatgag taagtatcca gaacggtgta cgacagttgg actgcgcttg aatgaagaat
    79981 cagattttga actctatgca ccttatggtt tggaggatat tttgaatttt caagttcgtc
    80041 caactcctca tttcttagaa aatgaagacc gaatggaact ctatcaaaca cgtttatcca
    80101 agaaaaattg gcaggagaaa tggaaaaatt tgatttttaa aaatacttaa ggaaacttta
    80161 agctagggag tgtacactaa gttcataagt taagaagacc ttaacttaaa ctcctaaaac
    80221 tttttcataa taatctccct ataaaaataa agtcgcctaa tcaggcggct tatttttttg
    80281 aaaaatgggc ttggtgcctg agaataaata gcttagtgat agaagaaaat ggggaaatat
    80341 ggtataatga aacgatagat ttttgaatag gaataagatc atgtttggat tttttaagaa
    80401 agataaggct gtggaagtag aggttccgac acaggttcct gctcatatcg gcatcatcat
    80461 ggatggcaat ggccgttggg ctaaaaaacg tatgcaaccg cgagtttttg gacacaaggc
    80521 gggcatggaa gcattgcaaa ccgtgaccaa ggcagccaac aaactgggcg tcaaggttat
    80581 tacggtctat gctttttcta cggaaaactg gacccgtcca gatcaggaag tcaagtttat
    80641 catgaacttg ccagtagagt tttatgataa ttatgtcccg gaactacatg cgaataatgt
    80701 taagattcaa atgattgggg agacagaccg cctgcctaag caaacctttg aagctttaac
    80761 caaggctgag gaattgacta agaacaacac aggattgatt cttaattttg ctcttaacta
    80821 tggtggacgt gctgagatta cacaggcgct taagttgatt tcccaggatg ttttagatgc
    80881 caaaatcaac ccaggtgaca tcacagagga attgattggt aactatctct ttactcagca
    80941 tttgcctaag gacttacgag acccagactt gattatccgt actagtggag aattacgttt
    81001 gagcaatttc cttccatggc agggagccta tagtgagctt tattttacgg acaccttatg
    81061 gcctgatttt gacgaagcag ccttgcagga agctattctt gcctataatc gtcgccatcg
    81121 ccgatttgga ggagtttagg aggaaatatg acccaggatt tacagaaaag aaccttgttt
    81181 gcagggattg ccctggctat tttcctacca attttaatga ttgggggcct cttgcttcag
    81241 atagcaatcg gaatcatagc catgctagcc atgcatgaac ttttgaagat aagaggtcta
    81301 gcgaccatga cgatggaggg cctcttgact ctctttgcaa cctttgcatt aaccattccc
    81361 ttggagaatt acctgacttt tttgccagtt gatgggaatg tggttgccta tagtgttttg
    81421 atttcaatca tgttaggaac gaccgttttt agcaagtctt atacgattga ggatgcggtt
    81481 ttccctcttg ctatgagctt ctacgtgggc tttggattta atgctttact agatgctcgt
    81541 gttgcaggtt tggacaaggc tctcttagcc ttgtgtatcg tctgggcgac agacagtggt
    81601 gcctatcttg ttgggatgaa ctatgggaaa cgaaagttag caccaagggt atcgcctaat
    81661 aaaacccttg agggtgcctt gggtggtatt ttaggagcaa ttttagtaac cattatcttt
    81721 atgatagttg acagtacagt tgctcttcca tatggaattt acaagatgtc agtctttgct
    81781 attttcttta gcattgctgg acaatttggt gatttactag aaagttcgat caaacgtcat
    81841 tttggtgtta aggattctgg gaaatttatc cctggacatg gtggtgtttt ggatcgtttc
    81901 gatagtatgt tgcttgtatt tccaatcatg cacttatttg gactctttta atcaaaagac
    81961 ggaggaaacg ctatgctcgg aattttaacc tttattctgg ttttcgggat tattgtagtg
    82021 gtgcacgagt tcgggcactt ctactttgcc aagaaatcag ggattttagt acgtgaattt
    82081 gccatcggta tgggacctaa aatttttgct cacattggca aggatggaac ggcctatacc
    82141 attcgaatct tgcctctggg tggctatgtc cgcatggccg gttggggtga tgatacaact
    82201 gaaatcaaga caggaacgcc tgttagtttg acacttgctg atgatggtaa ggttaaacgc
    82261 atcaatctct caggtaaaaa attggatcaa acagccctcc ctatgcaggt gacccagttt
    82321 gattttgaag acaagctctt tatcaaagga ttggttctgg aagaagaaaa aacatttgca
    82381 gtggatcacg atgcaacggt tgtggaagca gatggtactg aggttcggat tgcaccttta
    82441 gatgttcaat atcaaaatgc gactatctgg ggcaaactga ttaccaattt tgcaggtcct
    82501 atgaacaatt ttatcttagg tgtcgttgtt ttttgggttt taatctttat gcagggtggt
    82561 gtcagagatg ttgataccaa tcagttccat atcatgcccc aaggtgcctt ggccaaggta
    82621 ggagtaccag aaacggcaca aattaccaag attggctcac atgaggttag caactgggaa
    82681 agcttgatcc aagctgtgga aacagaaacc aaagataaga cggcaccgac tttggatgtg
    82741 actatttctg aaaaggggag tgacaaacaa gtcactgtta cacccgaaga tagtcaaggt
    82801 cgttaccttc taggtgttca accgggggtt aagtcagatt ttctatccat gtttgtaggt
    82861 ggttttacaa ctgctgctga ctcagctctc cgaattctct cagctctgaa aaatctgatt
    82921 ttccaaccgg atttgaacaa gttgggtgga cctgttgcta tctttaaggc aagtagtgat
    82981 gctgctaaaa atggaattga gaatatcttg tacttcttgg caatgatttc catcaatatt
    83041 gggattttta atcttattcc gattccagcc ttggatggtg gtaagattgt gctcaatatc
    83101 ctagaagcca tccgccacaa accattgaaa caagaaattg aaacctatgt caccttggcc
    83161 ggagtggtca tcatggttgt cttgatgatt gctgtgactt ggaatgacat tatgcgactc
    83221 ttttttagat aatcgaggaa tattatgaaa caaagtaaaa tgcctatccc aacgcttcgc
    83281 gaaatgccaa gcgatgctca agttatcagc catgctctta tgttgcgtgc tggttatgtt
    83341 cgccaagttt cagcaggtgt ttattcttat ctaccacttg ccaaccgtgt gattgaaaaa
    83401 gctaaaaaca tcatgcgcca agaattcgaa aagattggtg ctgttgagat gttggctcca
    83461 acccttctta gtgcagaatt gtggcgtgaa tcaggtcgtt acgaaaccta tggtgaagac
    83521 ctttacaaac taaaaaaccg tgaaaaatca gactttatct taggtccaac tcacgaagaa
    83581 acctttacag ctattgtccg tgattctgtt aaatcttaca agcaattgcc actcaacctt
    83641 tatcaaattc agcccaagta tcgtgatgaa aaacgcccac gtaatggact tcttcgtaca
    83701 cgtgagttta tcatgaagga tgcttatagt ttccacgcta actatgatag tttggatagt
    83761 gtttatgatg agtacaaagc agcctatgag cgtattttca ctcgtagtgg tttagacttc
    83821 aaggctatta ttggtgacgg tggagccatg ggtggtaagg atagccaaga atttatggcc
    83881 attacatctg ctcgtacaga ccttgaccgc tgggttgtct tggacaagtc agttgcctca
    83941 tttgacgaaa ttcctgcaga agtgcaagaa gaaatcaagg cagaattgct caaatggata
    84001 gtctctggtg aagataccat tgcttactca agtgagtcta gctatgcagc taacttagaa
    84061 atggcaacaa acgagtacaa accaagcaac cgtgttgtcg ctgaagaaga agttactcgt
    84121 gttgcaacgc cagatgttaa atcaattgat gaagttgcag ccttcctcaa tgttccagaa
    84181 gaacaaacga ttaaaaccct cttctacatt gcagatggtg agcttgttgc agcccttcta
    84241 gttggaaatg accaactcaa cgaagtcaag ttgaaaaatc acttgggagc agatttcttt
    84301 gacgttgcta gcgaagaaga agtggcgaat gttgttcaag caggatttgg ttcacttgga
    84361 ccagttggtt tgccagagaa tattaaaatt attgcagatc gtaaggtgca agatgttcgc
    84421 aatgcagttg tcggtgctaa cgaagatggc taccacttga ctggtgtgaa cccaggccgt
    84481 gattttactg cagaatatgt ggatatccgt gaagttcgtg agggtgaaat ttccccagat
    84541 ggacaaggtg tccttaactt tgcgcgtggt attgagatcg gtcatatttt caaactcgga
    84601 actcgctact cagcaagcat gggagcagat gtcttggatg aaaatggtcg tgctgtgcca
    84661 atcatcatgg gatgttacgg tatcggtgtc agccgtcttc tttcagcagt gatggagcaa
    84721 cacgctcgcc tctttgttaa caaaacgcca aaaggtgaat accgttacgc ttggggaatc
    84781 aatttcccta aagaattggc accatttgat gtgcatttga ttactgtcaa tgtcaaggat
    84841 gaagaagcgc aagccttgac agaaaaactt gaagcaagct tgatgggagc tggttatgaa
    84901 gtcttgacag atgaccgtaa cgaacgtgtc ggtgttaaat ttagcgatag tgacttgatt
    84961 ggtcttccaa tccgtatcac tgttggtaaa aaagcggccg atggcattgt agaagttaag
    85021 atcaaggcaa ctggtgacac cattgaggtt catgcagata acgtgcttga aacgcttgaa
    85081 atcctaagca agaaataaaa actataatca gaagaaaaac aaggaaaaaa tgtaactagt
    85141 ttttaccttg ttttttctgt ataatgggag aaatgagtag ataaagaggt aaatgttatg
    85201 ctaagatttc caaaggattt tgtctgggga tcctctactt ctggaccgca gacagaagga
    85261 cgtgtagctg gtgacggtaa gggagacaat ctctgggatt actggttcca agtggagcca
    85321 aatcgttact ataatgggat tggttcagat aagacatcga ctttttatga aaattgggag
    85381 cgggatattg agcttttgtt agagactggt cacacagtct ttcggacttc tattcagtgg
    85441 tcacggattt ttccacaagg ctgtggaaaa gtcaaccctc aaggtgtgga tttttatcgt
    85501 aaggtctttg aggctattaa ggctaaagga attcgtctgt tagtcaatct ctatcatttt
    85561 gatttacctt ttgctcttca agaggatggt gatggttggg aaaataaggc gacagtctca
    85621 gcctatgaag actatgctcg tttttgtttt gagacttatg gagatttagt ggatcaatgg
    85681 attaccttta acgagcccat cgttcctgta gaatttggtt atttttatga tgcccattat
    85741 ccacataagg tggatgcaga ggcagctgtt aaggtagcct atcatacaca attggccagc
    85801 agccgggctg ttaaggcttg ccatgaactt ttgcctgatt ccaagattgg gattgtcctc
    85861 aacttgacac cggcttatcc acgtagccag catcctgctg atgtcaaggc agctcgtatt
    85921 gcggcccttt ttcaggccca atctttctta gatccatctg tcttggggac ttatccacag
    85981 gagttggtag aaatcttgca tgaacacggt cttttacctg atgctacaga ggaagagttg
    86041 gaactcattc gtgataatac ggtggacttc cttggtgtga actactatca acctttgcgt
    86101 gttatggctc ctcgatttgc taagcatcca gatagtccac tcttaccaga acatttttac
    86161 gagccttatg tgatgcctgg acgtaaaatc aattctcacc gtggttggga gatttatgag
    86221 caagggattt atgacatcgc ccaaaatatc aaggaaaatt atggcaatat tgagtggatg
    86281 ttgactgaga atggtatggg tgttgaaggg gaagaaaaat tccgtcaaga tggaatgatt
    86341 caagatgatt accgtattga ctttgtaaaa ggtcatcttc gtgaacttca ccgtgccatt
    86401 gaagatggtg ccaactgtaa gggctacttg atttggacct ttattgactg ctggtcatgg
    86461 ctcaacagct ataaaaatcg ctatggtttg gtcgaattag acttggaaac gcaagaacgt
    86521 cgtctgaaga aatcaggcca ctggttcaaa gaattaagcg ataataatgg attttaaaaa
    86581 ggactctgac tgattatccc aatcggtcag agttttttgc ttacaggagc ttaatttgaa
    86641 ctataccaat ttttactctt gacaagtgaa agaaacaagt atatactgtt tttgctgatt
    86701 ctataaaaga tgaattagac tataccaatt ttaaggagaa agcacagctt gcctgtgtcg
    86761 tatatactat gtgtggaatt gttggtgttg ttggaaacac aaatgcaact gatattttga
    86821 ttcaagggct tgaaaagctt gaataccgtg gctatgattc tgcgggaatt tttgtcctag
    86881 atggtgctga taaccatttg gtgaaggctg ttggtcgtat cgcagaattg tctgccaaga
    86941 cagctggtgt tgagggaaca actggtatcg gacatactcg ttgggcaact catgggaaac
    87001 caacggaaga caatgctcac ccacaccgct ctgagacaga acgttttgtc ttggttcaca
    87061 atggggtgat tgaaaactac cttgaaatta aagaagaata ccttgctggg caccacttca
    87121 aagggcaaac agatacggaa atcgccgtac atttgattgg aaaatttgcg gaagaagacg
    87181 gtctctcagt tcttgaagcc tttaaaaaag ctcttcatat tatccgtggt tcatatgcct
    87241 ttgccttgat tgactctgaa aatccagatg tcatctatgt agcgaaaaac aaatctccac
    87301 ttttgattgg tcttggggaa ggctacaata tggtctgctc agatgctatg gctatgattc
    87361 gtgaaaccaa ccaatacatg gaaattcatg accaagagtt ggtaatcgtc aaggctgata
    87421 gcgtggaagt tcaagactat gatggtaaca gtcgtgaacg tgctagctat actgcggaac
    87481 ttgacttgtc agatatcggt aagggaactt atccttacta catgcttaag gaaattgatg
    87541 agcaaccaac tgttatgcgt aaactcattc aagcctacac ggatgatgct ggtcaagtag
    87601 tgattgatcc tgctatcatt aaggctgttc aagacgcaga ccgcatctac atccttgcag
    87661 ctggaacatc ttaccatgca ggatttgctt ctaagaaaat gttggaagaa ttgacagata
    87721 caccagttga acttggcatc tcatctgagt ggggctacgg tatgccactt ctcagcaaga
    87781 aaccactctt catctttatc agccaatctg gtgaaacagc ggatagtcgt caagttttgg
    87841 tcaaggctaa tgaaatggga attccaagct taacagtgac aaacgttcca ggttcaaccc
    87901 tctcacgtga agccaactat accatgctcc ttcacgcagg tcctgaaatt gccgtggcat
    87961 caactaaagc ctatacagcg caaatcgcag cccttgcctt ccttgcaaaa gcagtcggag
    88021 aagcaaatgg taatgctaaa gcgcaagcct ttgacctggt tcatgaattg tcaatcgtag
    88081 ctcagtctat cgaatcaact ctttcagaga aagaaaccat tgaagccaag gttcgtgaac
    88141 ttcttgaaac aactcgtaac gccttttaca tcggacgtgg tcaagattac tacgtagcca
    88201 tggaagcaag tctcaaactc aaagagattt cttatatcca gtgtgaaggt tttgcggcag
    88261 gagaactcaa gcacggaacc attgccttga ttgaagaagg aacacctgtc ttggctctct
    88321 tgtcagatcc agtccttgcc aaccacactc gtggaaatat ccaagaggtg gcagcccgtg
    88381 gtgctaaggt cctcactatc gcagaagaaa atgttgctaa agataccgac gatatcgtcc
    88441 ttacgactgt acacccttac ctctcaccaa tttcaatggt cgtaccgacg caattggttg
    88501 cttactttgc aaccctccac cgtggcctcg atgtggataa accacgtaac cttgctaaat
    88561 cagtaacggt agaataagct aaaaaagtct agttatctag gctttttcta atgagcaaat
    88621 tggttgcttg tactcaagat tcgtgttaga ataatttttc aaattgaagg acagagatag
    88681 acaaggagaa tcacaatggt agaattggga atttcaacat ttggggaaat aacggagctt
    88741 gaagggactg gacaaactta cagtcatgct gaacgcattc gccagttggt ggcagagatt
    88801 gagctggctg acaaggttgg tttggacgta tatgggattg gcgagcacca tcgggcggat
    88861 tttgcagtat cagccccaga gattgtcctg gccgctgggg cagtcaatac caagaaaatc
    88921 cgtttgacca gtgcagtcag cattctgtca agcatggatc cgattcgttt gttccaacag
    88981 tatgccacta tcgatgcttt gtcaaatgga cgatcggaga ttatggctgg aaggggctct
    89041 ttcacggaat ctttccctct gtttggttat gacttgaaag actacgactc tctttttgat
    89101 gagaaattag acttgcttca gttagtcaat gaaaagacca agttagactg gcaaggtcga
    89161 ttgactcaaa cgattgctgg taaagaagtt tatcctcgcc cagttcagga caaattgccc
    89221 ttgtggatag ctacaggtgg tcatgtcgaa tcaacagtga agattgctca ggctggtcta
    89281 ccgattgtat atgctattat tggtggtaat ccacgttatt ttaaaaagtt gattcaggct
    89341 tatcgtgaga ttggaagcga agcgggccat gctgacaaag acttgaaagt gggagcccat
    89401 tcttgggggt ggattgcgga agatggtgag caggcggtga aagattattt ccatccgact
    89461 aagcaagtgg tggatgctat ttccaaagac cgtccgcact ggcaggaatt gcgttatgag
    89521 caatatttgg agcaggtagg gccaaatggc gccatgtttg ttggcaatcc agatcaggtg
    89581 gccgaaaaat tgattcgcat gatagaggat ttaggattgg accgcttcat gcttcatcta
    89641 ccgcttggtt ctatgcctca tgaccaggtt ctgagagcta ttgaactctt cggaacacaa
    89701 gttgcaccca aagtacgagc ttattttgct atgaaagagg cttaataaaa aatcctaatc
    89761 aagtttatta ggacaacaaa tattgtacaa aaattaatcc cccgagctat aatgcttggg
    89821 ggatttttct ataggagggc tagtttagtt ctctttttta ttttttagta agaaaccgag
    89881 accaaggagc gcaaggaggc tgattcctgc aaataggagt ttgttttcgt ttttgattcc
    89941 tgtatttgga agttcagctt gtttatctgg ggttgcaggt atattttcct tgctagagtt
    90001 ttctaccgat tcgtttcgaa ccgcttcttt tacagatgct tcttgtgctt cttgtgctgg
    90061 ttgttcagct tgtgaatcag ctgtagcttg gctaggttta ttttccgcat cagctacttt
    90121 ggcatctggt ttagtagaat caggtctagc ttctggagct gggtcttgat gtgcagggtg
    90181 agaagcttca ttttgatgtt caggcttgga aggggttgag tctggtttct cttctgcagt
    90241 tgactcatgg ctagttccat tttgagagac tcgaagaaca gtagctgtaa gggcattcaa
    90301 tttcaagcct ttttcagtcc attcaagtcc tttcgggttg gcaattttga ctgatcctgc
    90361 ttggttttca tctgccaaaa cttccgcatt tcttagatgt gcaaaggcag ttcccaaatt
    90421 aaattcgcga gctttttcat ccgcattgac aaagactgcg tagatatcgc cgtttggagc
    90481 agtgatttgg tagccaatca ctacatcctc tttttccaca ccattttggc ctgggacagt
    90541 gatgaggtgg acacggtctt tgatatcttg gagactctta agtcggaagg catctgtaga
    90601 ttgacgaagg gcaatcaaac ctttcatata gtcacggctc ttgacatttt caggataagc
    90661 tttaccatct gtagccttag tccagtcaaa cttgttgact gcgtcgctag aatcgtaaga
    90721 atcatggatg aagtaaggat agtcaaatgg gttgccgtcc ttatcacgca acaagtgaga
    90781 tttgtttgga accttatcct ctgctactgg agtcttgtag gctgggtcac ggaattgttt
    90841 agtacgtcca tattcctgac cggagtggat aaatggagtt ccttgagctg tcaagaccat
    90901 gagatttcca agtcgtaaac gacggtggat ttcagcatag ttctcagcct tgcttgggtc
    90961 ttttttgata gactgggcaa tgatgtcaaa gagggtcaag ttatcatggg ctgctatgta
    91021 ttggatgaca tctccagggc tgtcagcttc aaagttagtt ggttgagcaa tgagattttt
    91081 aaagatggtg ttgacatcac gcttgccacc tgtgataaag gcaggttgac cttcgtttgg
    91141 ataaccagat ttgaggttgt tacggatgtc atctgaaaag acagcgacag tatcggtatg
    91201 tttcatccaa tcttggtcag cagctttagt aggcatgttt tcatcaccgg cataggttct
    91261 ccaaccttca ccaagcatga tgaggtttgg attgagggcg cgtgcagcct tgtaagcttc
    91321 ttcgatagaa gcggcgtcat ggtctcccat catatcgaaa cggaatccat ccactttgta
    91381 ggtatcaact aggtatttga tagagtcaac taggagccgt ttggtcatat ggtgggttgt
    91441 tcccaagcgt ccaccaccaa agctagttcg aggtgtgcca tcggcatcca taaagtggta
    91501 gtagtttggc tctaggtctt caaagatatc gactttggct gtgtggttat aaacgacatc
    91561 taggatagct cccataccac gtttgtggat ttcgttgatg aggtttttaa attctgcgat
    91621 tcgtttttct ggattcttag gatcgcttga gtacatacca gtcaaggaga agtagttttg
    91681 agggtcatat ccccagttgt agttgctgtt gcttgaagcg tagtcagaca agcgttcatg
    91741 gtttttcaat tcattgacaa agtagtaaga caagactgga aggagctgga tatgggttac
    91801 acccaagtct ttgagatagt ctagtttttc aatgaaggct tcaaaagtcc caaatggttt
    91861 ggtcaagtct tttgcaatgg caggatctga agtgaagtca cgcacatgag cttcgtagat
    91921 aacggcgtct tcacgagtct tgaaattgtg aatcttacca taagtcaagt cttgaggtcc
    91981 gagtttagct ggatctacaa aggcggcttt agccacttta tgggtatcgt caattttggc
    92041 atcgtcgcta ttccaagcag caagagattt agcgtaagga tcgagtgcaa gaacagtttt
    92101 accttgacgc tcgatttggt attgataata gtagccagtg aaatctgtga ttccgagttt
    92161 gtttgtgctg tctagagttt gtttccaagt tcctctttcc cctttttcga gagcgacagt
    92221 tccaactact ttgtcagggt cattcttgtc gtagacaaca acagaaacct tatcagcact
    92281 tggtgaccaa agggtcaaat caacttgttt tccttcttct tttaggtcag ctcccagttt
    92341 gccatcatag ctgtatgtct catctttcag gtgccagctt gttttggtag tgaattggtc
    92401 ggaattgtag ctaacagtat aagggtgttt tgtgtcagag aaatctccgc tgtaggtcac
    92461 tttcttacca gcttcatcga ttgcaacatc ggtaatagtt accttgtttc ctaggtgatt
    92521 agtgatgttg gagtgtttga ggatatcttc ttttttagca ccgacaagtg ttgaaaagct
    92581 actttcaatg ctagaagtgc ctacgtgttg ggctcctgtc atacggatat catggacata
    92641 gtatggattt gtgtaaatcg attcatcatc gtcttttagg aaaatttggc tatgattttt
    92701 caaatctgtg aacttataat tttctttacg gattttcacg tcgtctcctt gtttgctctc
    92761 atctagtaat aaaaatccaa attctcttgc ggcttcatta agaggaatgt cgatatagcg
    92821 gccatatttg cctgtagccg taaagtctgt tccgtcaggc cattgagcgc tacttggatt
    92881 tttcacatct ccccagtacc agagagattt cttgtcatag ttgccatctg tgcggtagta
    92941 gttgacgcga acagttcctg caggctgtgg ctcgtaagag aaaaccttgt aatcttggtc
    93001 taaccaagct tcgttcattt ttggaactag tttttctaca gatttatcgc cggttagatt
    93061 ttttccagct gtattgttga tgaggaagct aattttcttg gcttgttctc cctttaattt
    93121 gacatctagg taatagccgt agtcatcttt cttggcatcc ttgaaggaca aagctccgtt
    93181 tggccagttt tcagatggtt tttcaacatc gtcccaagtc catagtcctt gagcatcctt
    93241 gttttcttca ggaagttttt tgacatggat acggaagtag ttatcttcga tgggctcttc
    93301 tgccttagtt tcagttgtta ctggactagt agaagtagtt ggctctgttg caggtttttc
    93361 ttctgttttc tctagggaag cgtttgcatt tctaggtgaa gaaatatcac tttcgttctt
    93421 accagagcca gctgctgtat cagtaagagg ttgggcaagg gtggttgtat ttctgttgtt
    93481 gggttcgtta gtggttggtg tactttcgtc ggatgagata gttggtgtag ccatggcaag
    93541 caagacaagg cttgcgccga taaggacaga accagttcca tttttgaggg aacggatgct
    93601 gtagaccatt tttttctcag tgtgagatgg tgtttttctc ataatgattc tccaatcaat
    93661 aaatttctat atcagtatag catgtagtaa aacaatatgc aagcgttttc tttgatttgt
    93721 gaaaaaattc ttacttttga gaagttttct gtactatttt atatatcagt ataaatgacg
    93781 gagataaaaa tgtcacataa gccaagccgt attattagga agatgatgtg ttcccagcta
    93841 tttttattca gaaattgatc tctaacatta ccaataaaaa agaaaaatat cttgacaagc
    93901 aagggaaaat tttgttacaa taatagacgg tactttttac ttttggtctc tcaagagtgt
    93961 acagggacgt gctgacaaat gttgcaaaag tacacacaga tgatatctgt caccaagtgt
    94021 atcatcacca aaaataaaaa aacacaggag aatgtagatg cctacaatta accaattggt
    94081 tcgcaaaccg cgtaaatcaa aagtagaaaa atctaaatca ccagctttga acgttggtta
    94141 caatagtcat aaaaaagttc aaacaaacgt ttcttcacca caaaaacgtg gtgttgcaac
    94201 tcgtgttgga acaatgacac ctaaaaaacc taactcagcc cttcgtaaat tcgctcgtgt
    94261 acgtttgagc aaccttatcg aagttactgc ctacatccca ggtatcggac acaacttgca
    94321 agagcacagc gtggtgcttc ttcgcggtgg acgtgtaaaa gaccttccag gggtacgtta
    94381 ccatatcgtc cgtggtgcac ttgatactgc aggtgttaac gatcgtaaac aaggccgttc
    94441 taaatacggt actaaacgtc caaaagcata aggaaagggg ataaagagaa atgagtcgta
    94501 aaaatagagc tccaaaacgt gacgtattgc cagatccgct atacaattca caactagtta
    94561 ctcgtcttat caaccgcgtt atgcttgatg gtaaacgtgg tactgctgct tcaatcgttt
    94621 acggtgcttt tgagcaaatc aaagaagcta ctggcaacga tgcacttgaa gtatttgaaa
    94681 cagctatgga aaacatcatg cctgtacttg aagtacgtgc acgtcgtgtt ggtggttcta
    94741 actaccaagt cccagttgaa gttcgtccag aacgtcgtac aacacttgga cttcgttggt
    94801 tggtaacaat cgctcgtctt cgtggtgaac acacaatgca agaccgtctt gcaaaagaaa
    94861 tcttggatgc tgctaacaac actggtgcag cagttaagaa acgtgaagat actcaccgta
    94921 tggctgaagc taaccgtgca ttcgcacact tccgttggta agataggatg cgaaagcgtt
    94981 aagaaagtcc cagagaaaat agggaatcga agcaggttgc ggttgcaacc aatgagattc
    95041 atctttttct ccagactttt agcttgagct caactaaatc atgatgctag gcacggaagg
    95101 atgcaaggtg aaaataggaa actgacgcag tattcaacga atacaaggag ttttatcttt
    95161 ttcacgcagc atcccgttcc agctcacatc ggctaactaa ctttagcctg ggttcaaatt
    95221 agctaaatcg attagtatta gctataactc agcttaccat ctcgtaagtt gaaaccaaca
    95281 atagcatgaa aacattgaga acgggtaggt cctgcctatc cgtttttatt aaaatcgtgt
    95341 tataatagaa tagaaatcaa aaataaatag gagaaacaaa cctcatggca cgcgaatttt
    95401 cacttgaaaa aactcgtaat atcggtatca tggctcacgt cgatgccggt aaaacaacaa
    95461 ctactgagcg tattctttac tacactggta aaatccacaa aatcggtgaa actcacgaag
    95521 gtgcgtcaca aatggactgg atggagcaag agcaagaacg tggtatcacg atcacatctg
    95581 ctgcgacaac agctcaatgg aacaaccacc gcgtaaacat catcgacaca ccaggacacg
    95641 tggacttcac aatcgaagta caacgttctc ttcgtgtatt ggatggtgcg gttaccgttc
    95701 ttgactcaca atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaactgagt
    95761 acggagttcc acgtatcgta tttgccaaca aaatggacaa aatcggtgct gacttccttt
    95821 actctgtaag cacacttcac gatcgtcttc aagcaaatgc acacccaatc caattgccaa
    95881 tcggttctga agatgacttc cgtggtatca ttgacttgat caagatgaaa gctgaaatct
    95941 atactaacga ccttggtacg gatatccttg aagaagacat cccagctgaa taccttgacc
    96001 aagctcaaga ataccgtgaa aaattgattg aagcagttgc tgaaactgac gaagaattga
    96061 tgatgaaata cctcgaaggt gaagaaatca ctaacgaaga attgaaagct ggtatccgta
    96121 aagcgactat caacgttgaa ttcttcccag tattgtgtgg ttcagccttc aaaaacaaag
    96181 gtgttcaatt gatgcttgat gcggttatcg actaccttcc aagtccactt gacatcccag
    96241 caatcaaagg tattaaccca gatacagacg ctgaagaaac tcgtccagca tctgacgaag
    96301 agccatttgc agctcttgcc ttcaagatca tgactgaccc attcgtaggt cgtttgacat
    96361 tcttccgtgt ttactcaggt gttcttcaat caggttcata cgtattgaat acttctaaag
    96421 gtaaacgtga acgtatcgga cgtatccttc aaatgcacgc taacagccgt caagaaatcg
    96481 acactgttta ctcaggtgat atcgctgctg ccgttggttt gaaagatact acaactggtg
    96541 actcattgac agatgaaaaa gctaaaatca tccttgagtc aatcaacgtt ccagaaccag
    96601 ttatccaatt gatggttgag ccaaaatcta aagctgacca agacaagatg ggtatcgccc
    96661 ttcaaaaatt ggctgaagaa gatccaacat tccgcgttga aacaaacgtt gaaactggtg
    96721 aaacagttat ctcaggtatg ggtgaacttc accttgacgt ccttgttgat cgtatgcgtc
    96781 gtgagttcaa agttgaagcg aacgtaggtg ctcctcaagt atcttaccgt gaaacattcc
    96841 gcgcttctac tcaagcacgt ggattcttca aacgtcagtc tggtggtaaa ggtcaattcg
    96901 gtgatgtgtg gattgaattt actccaaacg aagaaggtaa aggattcgaa ttcgaaaacg
    96961 caatcgtcgg tggtgtggtt cctcgtgaat ttatcccagc ggttgaaaaa ggtttggtag
    97021 aatctatggc taacggtgtt cttgcaggtt acccaatggt tgacgttaaa gctaagcttt
    97081 atgatggttc atatcacgat gtcgactcat ctgaaactgc cttcaagatt gcggcttcac
    97141 tttcccttaa agaagctgct aaatcagcac aaccagctat ccttgaacca atgatgcttg
    97201 taacaatcac tgttccagaa gaaaaccttg gtgatgttat gggtcacgta actgctcgtc
    97261 gtggacgtgt agatggtatg gaagcacacg gtaacagcca aatcgttcgt gcttacgttc
    97321 cacttgctga aatgttcggt tacgcaacag ttcttcgttc tgcatctcaa ggacgtggta
    97381 cattcatgat ggtatttgac cactacgaag atgtacctaa gtcagtacaa gaagaaatta
    97441 ttaagaaaaa taaaggtgaa gactaatccg tcctcactct agaaggaagt cacttagtgg
    97501 cttccttttg tctttagaaa atacctctaa atatggtaaa atagtagaag aataatgtga
    97561 ggaaaatgaa tgtcaaatag ttttgaaatt ttgatgaatc aattggggat gcctgctgaa
    97621 atgagacagg ctcctgcttt agcacaggcc aatattgagc gagttgtggt tcataaaatt
    97681 agtaaggtat gggagtttca tttcgtattt tctaatattt taccgattga aatcttttta
    97741 gaattaaaga aaggtttgag cgaagaattt tctaagacag gcaataaagc tgtttttgaa
    97801 attaaggctc ggtctcaaga attttcaaat cagctcttgc agtcctacta tagggaggct
    97861 ttctctgaag gtccatgtgc tagtcaaggt tttaagtccc tttatcaaaa tttgcaagtt
    97921 cgtgctgagg gtaatcagct atttattgaa ggatctgaag cgattgataa ggaacatttt
    97981 aagaagaatc atcttcctaa tttagccaaa caacttgaaa agtttggttt tccaactttt
    98041 aactgtcaag ttgagaagaa tgatgtcctg acccaagagc aggaagaggc ctttcatgct
    98101 gaaaatgagc agattgttca agctgccaat gaggaagcgc tccgtgctat ggaacaactg
    98161 gagcagatgg cacctcctcc agcggaagag aaaccagtct ttgattttca agcgaaaaaa
    98221 gctgcagcta aacccaagct ggataaggcg gagattactc ctatgatcga agtgacgaca
    98281 gaggaaaatc gtctggtatt tgaaggggtt gtttttgatg tggagcaaaa agtgactaga
    98341 acaggtcgtg ttttaatcaa ctttaaaatg acggactata cttcaagttt ttctatgcaa
    98401 aagtgggtta aaaacgagga agaggcccag aagtttgacc tcatcaagaa gaattcttgg
    98461 ctccgagttc gagggaatgt ggagatgaat aacttcacac gcgatttgac tatgaacgta
    98521 caggatctgc aggaagttgt tcactatgag cggaaggatt tgatgccaga aggtgagcgt
    98581 cgggttgagt ttcatgctca tactaacatg tcgactatgg atgctttgcc agaggtcgaa
    98641 gagattgttg caacagctgc taagtgggga cacaaggcgg ttgctatcac agaccatggg
    98701 aatgtccagt cctttccaca tggctataag gcggctaaga aagcgggaat ccagctgatc
    98761 tatgggatag aagccaatat cgtggaggac cgtgtcccta tcgtctataa cgaagtggag
    98821 atggacttat cagaagcaac ctacgtggtc tttgacgtgg aaacgacggg actttcagct
    98881 atctataatg acttgattca ggttgcggcc tctaagatgt acaaggggaa tgttattgct
    98941 gaatttgatg aatttatcaa tcctgggcat cccttgtcag cttttactac agagttaact
    99001 ggaattacag atgatcatgt caaaaatgcc aaaccactag aacaagtttt gcaagaattc
    99061 caagaatttt gcaaggatac ggtcctagtt gcccacaatg ctgcctttga cgttggcttt
    99121 atgaatgcta attatgagcg tcatggtctt ccaaagatta gtcagccagt tattgatacg
    99181 ctggagtttg ctagaaacct ctatcctgag tataaacgcc atggtttggg gcctttgacc
    99241 aagcgttttg gtgtggcctt ggaacatcac cacatggcca actacgatgc ggaagcgact
    99301 ggtcgtctgc ttttcatctt tatcaaagag gtagcagaaa aacatggtgt gaccgattta
    99361 gctagactca acattgatct aatcagtcca gattcttaca aaaaagctcg gatcaagcat
    99421 gcgaccatct atgtcaagaa tcaggtaggt ctaaaaaata tctttaagct ggtttccttg
    99481 tctaatacca agtattttga aggagtgcca cggattccga gaacggttct agatgcccat
    99541 cgagagggct tgattttagg ttcagcctgt tcagagggtg aagtttttga cgtggtcgtt
    99601 tctcaaggtg tggatgcggc ggttgaggtg gccaagtatt atgactttat cgaggtcatg
    99661 ccaccggcta tctatgcgcc cttgattgct aaagagcagg tcaaggatat ggaggaactc
    99721 cagaccatta tcaagagttt gatagaggtt ggagaccgcc ttggcaagcc tgttttggct
    99781 acgggaaatg ttcactatat cgaaccggaa gaagagattt atcgtgaaat tatcgtccgt
    99841 agtttgggac agggtgcgat gattaaccga actatcggtc atggtgaaca tgcccaacca
    99901 gcaccacttc caaaggctca ttttcgaacg actaatgaga tgttggatga atttgccttt
    99961 ttgggagagg aactggctcg taaactggtt attgaaaaca ccaatgcctt ggcagaaata
   100021 tttgaacccg ttgaagtcgt taagggtgac ttgtatacgc ctttcatcga caaggctgaa
   100081 gaaacagttg ctgagttgac ctataagaaa gcttttgaga tttatggaaa tccgctgcca
   100141 gatattgttg atttgcggat tgaaaaagaa ttaacatcca tactggggaa tggatttgct
   100201 gtgatttatc tggcatcgca gatgctggtg caacgttcta atgaacgggg ttatttggtt
   100261 ggttctcgtg ggtctgtcgg atctagtttc gttgcgacca tgattggaat tacggaggtc
   100321 aatcctctct ctcctcacta tgtctgtggt cagtgtcagt acagtgagtt tatcacagat
   100381 ggttcgtacg gttcaggatt tgatatgccc cataaggact gtccaaactg tggtcacaaa
   100441 ctcagtaaaa acggacagga tattccgttt gagaccttcc ttggttttga tggggataag
   100501 gttcctgata ttgacttgaa cttctcggga gaagatcagc ctagcgccca cttggatgtg
   100561 cgtgatatct ttggtgaaga atatgccttc cgtgcgggaa cagttggtac ggtagctgcc
   100621 aagactgcct atggatttgt caagggttac gagcgagatt atggcaagtt ttatcgtgat
   100681 gcagaagtag aacgcctcgc tcaaggagcg gcgggtgtca agcggacaac aggccaacac
   100741 ccggggggaa tcgttgttat tccgaactac atggatgtct acgattttac gcctgtccag
   100801 tatccagcag atgatgtcac ggctgaatgg cagaccactc actttaactt ccacgatatc
   100861 gatgagaacg tcctcaaact cgatgtactg ggacatgatg atccgaccat gattcgaaaa
   100921 cttcaggatt tgtctggtat tgaccctaat aaaattccta tggatgacga aggcgtgatg
   100981 gcactctttt ctgggactga tgtgctaggg gtaacacctg aacaaattgg aacgcctacg
   101041 ggtatgttgg ggattccaga gtttggaaca aatttcgtac gtggaatggt agacgaaacc
   101101 catccgacaa cctttgcgga attgcttcag ctgtctggtc tgtcccacgg tactgatgtt
   101161 tggttgggga atgctcagga tctgattaag caaggaatag cggacctatc gactgttatc
   101221 ggttgtcggg acgacatcat ggtttacctc atgcatgcgg gtctggaacc taagatggcc
   101281 tttaccatta tggaacgggt acgtaagggt ttgtggctaa agatttcaga agaggagaga
   101341 aatggctata tcgaagcaat gaaggctaat aaggtgccag agtggtatat cgaatcctgt
   101401 gggaaaatta agtacatgtt ccctaaggcc catgcggcag cctacgttat gatggccttg
   101461 cgtgtagctt acttcaaggt tcaccatcct atttattact actgtgctta cttctccatt
   101521 cgtgctaagg cttttgatat caagaccatg ggtgcgggct tggaggtcat caagcgcaga
   101581 atggaagaaa tctctgaaaa acggaagaac aatgaagcct ctaatgtgga aatcgatctc
   101641 tatacaactc ttgagattgt caatgagatg tgggaacgag gtttcaagtt tggtaaatta
   101701 gatctctacc gtagtcaggc gacagagttc ctcatcgacg gggataccct tatcccacca
   101761 tttgtagcaa tggatggtct gggagagaac gttgccaagc aactggtgcg ggcgcgtgaa
   101821 gagggagaat tcctctctaa aacagaacta cgcaagcgtg gtggactctc atcaaccttg
   101881 gttgaaaaga tggatgagat gggtattctt ggaaatatgc cagaggataa ccagttgagt
   101941 ttgtttgatg agttgtttta aaaaattgct taataatcta ttaaaagagg ctaacgtata
   102001 tccaatagat ttacattagc tttctttttt gttaaaatag tctatggaaa gagggtgaga
   102061 gtatgtcaaa gatgagtata agcatccgtc tggatagtga ggttaaggag caggcccaac
   102121 aggtgtttag taatctggga atggatatga caacagctat taatattttc cttcgtcagg
   102181 caattcaata tcagggatta ccttttgatg ttagactaga cgaaaatcgg aagttgctcc
   102241 aagcgttaac ggatttagac caaaatcgta atatgagcca gtcttttgaa tcagtctcag
   102301 atttgatgga ggacttacgt gcttaagatt cgttatcata aacagtttaa aaaagatttt
   102361 aagttggcta tgaagcgtgg tttgaaggca gaattattag aagaagtttt gaattttctg
   102421 gttcaagaaa aagaacatcc tgccagatat cgtgatcatt cattgacggc atccaagcat
   102481 tttcaaggag ttcgtgaatg ccatacccag ccagattggc ttttggttta taaagtagac
   102541 aagtcggaat tgattttaaa tttgctgagg acaggcagtc acagtgattt attttaatct
   102601 attttaaggg ggttctcatg aaactaagaa tatttgcgga agataagccg gctaagaagg
   102661 tatttgaata tcaattagaa cttgctgatc gtacaattct tctatcgaca gcactcttgt
   102721 caggtgctat tgctttagca ggaatctttt ctgctttgaa agaaaaataa aaatagaaaa
   102781 gagaaaacag aatggtttta ccaaatttta aagaaaatct agaaaaatat gcgaaattgt
   102841 tggttgcgaa cggaattaac gtgcaacctg gtcacacttt ggctctctct attgatgtgg
   102901 agcaacgtga attggcacat ctaatcgtga aagaagctta tgccttgggt gcgcatgagg
   102961 tcatcgttca gtggacagat gatgtgatta accgtgagaa attcctccat gccccgatgg
   103021 agcgtttgga caatgtgcca gaatacaaga ttgctgagat gaactatctt ttggagaaca
   103081 aggctagccg tcttggggtt cgttcatctg atccaggtgc cttgaacgga gtggacgctg
   103141 acaagctttc agcttctgct aaagctatgg gacttgccat gaagcctatg cgtatcgcaa
   103201 ctcaatctaa caaggttagc tggactgtag cagccgctgc tggacttgag tgggctaaga
   103261 aagtcttccc aaatgctgcg agcgacgaag aagcagttga tttcctttgg gatcaaatct
   103321 tcaaaacttg tcgtgtctac gaagcagatc ctgttaaggc ttgggaggaa catgcagcca
   103381 ttctcaagag caaggccgat atgcttaata aggagcaatt ttcagccctt cactacacag
   103441 cgccaggaac agatttaaca cttggtttgc caaagaacca cgtttgggaa tcagctggtg
   103501 ctgtcaatgc acagggcgaa gaattcttgc caaatatgcc gacagaagag gtcttcacag
   103561 cgcctgactt ccgtcgtgca gatggttatg tcacttctac aaaaccgctt agctacaacg
   103621 gaaatatcat tgaaggcatt aaggtgacct ttaaggatgg acaaatcgta gatatcactg
   103681 ctgagaaggg tgatcaggtt atgaaagacc ttgtctttga aaatgcgggt gcgcgtgcct
   103741 tgggtgaatg tgccttggta ccagatccaa gtccaatttc tcagtcaggc attacctttt
   103801 ttaacaccct tttcgatgaa aatgcgtcaa accacttggc tatcggtgca gcctatgcga
   103861 ctagcgttgt tgatggagcg gagatgagcg aagaggagct tgaagctgca gggcttaacc
   103921 gttcagatgt tcacgtagac tttatgattg gttctaacca aatggatatc gatggtattc
   103981 gtgaggatgg aacgcgggta cctcttttcc gtaatgggaa ttgggcaaat taaggagata
   104041 atatgttagg aagtatgttc gttggtctcc tagtgggatt tttagcaggt gctatgacca
   104101 atcgtggaga gcgaatggga tgttttggaa aaatgtttct cggttggatc ggagcctttc
   104161 taggtcactt gctctttgga acttgggggc cagttttatc aggaacagct attatcccag
   104221 cgattttagg agccatgatt gttttagcta ttttttggag acgaggaagt taatttccta
   104281 aatctgatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gttgctcaaa
//
LOCUS       DBERYS010000002        97300 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.002, whole genome shotgun sequence.
ACCESSION   DBERYS010000002 DBERYS010000000
VERSION     DBERYS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 97300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.002"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(18..983)
                     /locus_tag="ACL8LA_000112"
     CDS             complement(18..983)
                     /locus_tag="ACL8LA_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1811945.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESIKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(1079..1831)
                     /locus_tag="ACL8LA_000113"
     CDS             complement(1079..1831)
                     /locus_tag="ACL8LA_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1811946.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(1828..2784)
                     /locus_tag="ACL8LA_000114"
     CDS             complement(1828..2784)
                     /locus_tag="ACL8LA_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGS1811947.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(2774..3733)
                     /locus_tag="ACL8LA_000115"
     CDS             complement(2774..3733)
                     /locus_tag="ACL8LA_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1811948.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(4030..4140)
                     /locus_tag="ACL8LA_000116"
     CDS             complement(4030..4140)
                     /locus_tag="ACL8LA_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGS1811949.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            complement(4156..4857)
                     /gene="galE"
                     /locus_tag="ACL8LA_000117"
     CDS             complement(4156..4857)
                     /gene="galE"
                     /locus_tag="ACL8LA_000117"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1811950.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            5258..6322
                     /gene="pepA"
                     /locus_tag="ACL8LA_000118"
     CDS             5258..6322
                     /gene="pepA"
                     /locus_tag="ACL8LA_000118"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGS1811951.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            6344..6640
                     /locus_tag="ACL8LA_000119"
     CDS             6344..6640
                     /locus_tag="ACL8LA_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS1811952.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            7322..7765
                     /locus_tag="ACL8LA_000120"
     CDS             7322..7765
                     /locus_tag="ACL8LA_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS1811953.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            7769..8533
                     /locus_tag="ACL8LA_000121"
     CDS             7769..8533
                     /locus_tag="ACL8LA_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGS1811954.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            8548..9276
                     /locus_tag="ACL8LA_000122"
     CDS             8548..9276
                     /locus_tag="ACL8LA_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1811955.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            9269..10789
                     /locus_tag="ACL8LA_000123"
     CDS             9269..10789
                     /locus_tag="ACL8LA_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGS1811956.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            11134..11940
                     /gene="pnuC"
                     /locus_tag="ACL8LA_000124"
     CDS             11134..11940
                     /gene="pnuC"
                     /locus_tag="ACL8LA_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGS1811957.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(12106..12642)
                     /gene="sczA"
                     /locus_tag="ACL8LA_000125"
     CDS             complement(12106..12642)
                     /gene="sczA"
                     /locus_tag="ACL8LA_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HGS1811958.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            12775..13665
                     /gene="czcD"
                     /locus_tag="ACL8LA_000126"
     CDS             12775..13665
                     /gene="czcD"
                     /locus_tag="ACL8LA_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGS1811959.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            13909..14262
                     /gene="nmlR"
                     /locus_tag="ACL8LA_000127"
     CDS             13909..14262
                     /gene="nmlR"
                     /locus_tag="ACL8LA_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGS1811960.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            14263..15300
                     /locus_tag="ACL8LA_000128"
     CDS             14263..15300
                     /locus_tag="ACL8LA_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGS1811961.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(15347..16354)
                     /gene="galR"
                     /locus_tag="ACL8LA_000129"
     CDS             complement(15347..16354)
                     /gene="galR"
                     /locus_tag="ACL8LA_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGS1811962.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            16519..17697
                     /locus_tag="ACL8LA_000130"
     CDS             16519..17697
                     /locus_tag="ACL8LA_000130"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGS1811963.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            17716..19197
                     /locus_tag="ACL8LA_000131"
     CDS             17716..19197
                     /locus_tag="ACL8LA_000131"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS1811964.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            19267..19671
                     /locus_tag="ACL8LA_000132"
     CDS             19267..19671
                     /locus_tag="ACL8LA_000132"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGS1811965.1"
                     /translation="MKDFHFDAISAFESYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            19852..20616
                     /locus_tag="ACL8LA_000133"
     CDS             19852..20616
                     /locus_tag="ACL8LA_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HGS1811966.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            20613..21074
                     /locus_tag="ACL8LA_000134"
     CDS             20613..21074
                     /locus_tag="ACL8LA_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HGS1811967.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(21125..22387)
                     /locus_tag="ACL8LA_000135"
     CDS             complement(21125..22387)
                     /locus_tag="ACL8LA_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGS1811968.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGNGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(22387..22968)
                     /locus_tag="ACL8LA_000136"
     CDS             complement(22387..22968)
                     /locus_tag="ACL8LA_000136"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGS1811969.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(23368..23463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(23846..24085)
                     /locus_tag="ACL8LA_000137"
     CDS             complement(23846..24085)
                     /locus_tag="ACL8LA_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGS1811970.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(24133..24228)
                     /locus_tag="ACL8LA_000138"
                     /pseudo
     CDS             complement(24133..24228)
                     /locus_tag="ACL8LA_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
     gene            complement(24240..25067)
                     /locus_tag="ACL8LA_000139"
     CDS             complement(24240..25067)
                     /locus_tag="ACL8LA_000139"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGS1811971.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            25201..25657
                     /locus_tag="ACL8LA_000140"
                     /pseudo
     CDS             25201..25657
                     /locus_tag="ACL8LA_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            25654..26184
                     /locus_tag="ACL8LA_000141"
     CDS             25654..26184
                     /locus_tag="ACL8LA_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGS1811972.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            26312..28057
                     /locus_tag="ACL8LA_000142"
     CDS             26312..28057
                     /locus_tag="ACL8LA_000142"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1811973.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            28047..29798
                     /locus_tag="ACL8LA_000143"
     CDS             28047..29798
                     /locus_tag="ACL8LA_000143"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1811974.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            29971..30663
                     /locus_tag="ACL8LA_000144"
     CDS             29971..30663
                     /locus_tag="ACL8LA_000144"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1811975.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            30706..31932
                     /locus_tag="ACL8LA_000145"
     CDS             30706..31932
                     /locus_tag="ACL8LA_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGS1811976.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            32553..32783
                     /locus_tag="ACL8LA_000146"
     CDS             32553..32783
                     /locus_tag="ACL8LA_000146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811977.1"
                     /translation="MSDIQVNIPGECLYDKVFVLSFIIYNISTNLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVK"
     gene            33063..35222
                     /gene="pfbA"
                     /locus_tag="ACL8LA_000147"
     CDS             33063..35222
                     /gene="pfbA"
                     /locus_tag="ACL8LA_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGS1811978.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            35206..35502
                     /locus_tag="ACL8LA_000148"
     CDS             35206..35502
                     /locus_tag="ACL8LA_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811979.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            35674..36057
                     /locus_tag="ACL8LA_000149"
     CDS             35674..36057
                     /locus_tag="ACL8LA_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811980.1"
                     /translation="MLVNEMEITDHRVDNLFEKGKNEIKDPIGTNSVLNKKIILQKIR
                     KLSNQPSGYWIGSLDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELIK
                     MRFNSSAIDPIYGKKDDASIVVIDV"
     gene            36115..36756
                     /locus_tag="ACL8LA_000150"
     CDS             36115..36756
                     /locus_tag="ACL8LA_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HGS1811981.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLDKYEKLFEYLSNEERIELI
                     KEDLIINESVLNVDKKGYELICLQHPVSYDLRRIISVIKISTDIERIGDRIVEILKNL
                     QIIQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELST
                     NIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            36769..38244
                     /gene="galT"
                     /locus_tag="ACL8LA_000151"
     CDS             36769..38244
                     /gene="galT"
                     /locus_tag="ACL8LA_000151"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS1811982.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            38257..39237
                     /gene="galE"
                     /locus_tag="ACL8LA_000152"
     CDS             38257..39237
                     /gene="galE"
                     /locus_tag="ACL8LA_000152"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1811983.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGLPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            39447..40106
                     /locus_tag="ACL8LA_000153"
     CDS             39447..40106
                     /locus_tag="ACL8LA_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGS1811984.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            40131..41198
                     /locus_tag="ACL8LA_000154"
     CDS             40131..41198
                     /locus_tag="ACL8LA_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1811985.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELASKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWGVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            41301..42311
                     /locus_tag="ACL8LA_000155"
     CDS             41301..42311
                     /locus_tag="ACL8LA_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1811986.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            42323..44014
                     /locus_tag="ACL8LA_000156"
     CDS             42323..44014
                     /locus_tag="ACL8LA_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1811987.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            44017..44727
                     /locus_tag="ACL8LA_000157"
     CDS             44017..44727
                     /locus_tag="ACL8LA_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGS1811988.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            44823..45191
                     /locus_tag="ACL8LA_000158"
     CDS             44823..45191
                     /locus_tag="ACL8LA_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811989.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            45299..46300
                     /locus_tag="ACL8LA_000159"
     CDS             45299..46300
                     /locus_tag="ACL8LA_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1811990.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    46512..46741
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            46907..47158
                     /locus_tag="ACL8LA_000160"
     CDS             46907..47158
                     /locus_tag="ACL8LA_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811991.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKYCFEVVDRTDEVS
                     SKHCFEVADRTDEVSNHTYGKATLTRFEEIFEEYKGVSK"
     gene            47531..48892
                     /gene="trpE"
                     /locus_tag="ACL8LA_000161"
     CDS             47531..48892
                     /gene="trpE"
                     /locus_tag="ACL8LA_000161"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGS1811992.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            48889..49455
                     /locus_tag="ACL8LA_000162"
     CDS             48889..49455
                     /locus_tag="ACL8LA_000162"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGS1811993.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            49466..50470
                     /gene="trpD"
                     /locus_tag="ACL8LA_000163"
     CDS             49466..50470
                     /gene="trpD"
                     /locus_tag="ACL8LA_000163"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGS1811994.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDVRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            50467..51234
                     /gene="trpC"
                     /locus_tag="ACL8LA_000164"
     CDS             50467..51234
                     /gene="trpC"
                     /locus_tag="ACL8LA_000164"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGS1811995.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLNYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            51221..51820
                     /locus_tag="ACL8LA_000165"
     CDS             51221..51820
                     /locus_tag="ACL8LA_000165"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGS1811996.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            51798..53021
                     /gene="trpB"
                     /locus_tag="ACL8LA_000166"
     CDS             51798..53021
                     /gene="trpB"
                     /locus_tag="ACL8LA_000166"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGS1811997.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            53014..53790
                     /gene="trpA"
                     /locus_tag="ACL8LA_000167"
     CDS             53014..53790
                     /gene="trpA"
                     /locus_tag="ACL8LA_000167"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGS1811998.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(53762..53863)
                     /locus_tag="ACL8LA_000168"
                     /pseudo
     CDS             complement(53762..53863)
                     /locus_tag="ACL8LA_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            54092..54823
                     /locus_tag="ACL8LA_000169"
     CDS             54092..54823
                     /locus_tag="ACL8LA_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1811999.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            54838..55989
                     /locus_tag="ACL8LA_000170"
     CDS             54838..55989
                     /locus_tag="ACL8LA_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1812000.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(56041..56700)
                     /locus_tag="ACL8LA_000171"
     CDS             complement(56041..56700)
                     /locus_tag="ACL8LA_000171"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGS1812001.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTCHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            56770..57216
                     /locus_tag="ACL8LA_000172"
     CDS             56770..57216
                     /locus_tag="ACL8LA_000172"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812002.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
     gene            complement(57444..58622)
                     /locus_tag="ACL8LA_000173"
     CDS             complement(57444..58622)
                     /locus_tag="ACL8LA_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS1812003.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            58902..60164
                     /locus_tag="ACL8LA_000174"
     CDS             58902..60164
                     /locus_tag="ACL8LA_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1812004.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            60167..61063
                     /locus_tag="ACL8LA_000175"
     CDS             60167..61063
                     /locus_tag="ACL8LA_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HGS1812005.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            61078..61395
                     /locus_tag="ACL8LA_000176"
     CDS             61078..61395
                     /locus_tag="ACL8LA_000176"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1812006.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            61412..61729
                     /locus_tag="ACL8LA_000177"
     CDS             61412..61729
                     /locus_tag="ACL8LA_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1812007.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            61739..63094
                     /locus_tag="ACL8LA_000178"
     CDS             61739..63094
                     /locus_tag="ACL8LA_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1812008.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLNQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWHAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            63417..64892
                     /locus_tag="ACL8LA_000179"
     CDS             63417..64892
                     /locus_tag="ACL8LA_000179"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HGS1812009.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVNFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            64889..65737
                     /locus_tag="ACL8LA_000180"
     CDS             64889..65737
                     /locus_tag="ACL8LA_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HGS1812010.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDNFEDPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTGLDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            65752..66522
                     /locus_tag="ACL8LA_000181"
     CDS             65752..66522
                     /locus_tag="ACL8LA_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGS1812011.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            67325..67564
                     /locus_tag="ACL8LA_000182"
     CDS             67325..67564
                     /locus_tag="ACL8LA_000182"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1812012.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFILMANNLLKYNKRKRQN"
     gene            complement(67674..67877)
                     /locus_tag="ACL8LA_000183"
     CDS             complement(67674..67877)
                     /locus_tag="ACL8LA_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS1812013.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(67908..68516)
                     /locus_tag="ACL8LA_000184"
     CDS             complement(67908..68516)
                     /locus_tag="ACL8LA_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1812014.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(68555..68725)
                     /locus_tag="ACL8LA_000185"
     CDS             complement(68555..68725)
                     /locus_tag="ACL8LA_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGS1812015.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(68737..69303)
                     /gene="amaP"
                     /locus_tag="ACL8LA_000186"
     CDS             complement(68737..69303)
                     /gene="amaP"
                     /locus_tag="ACL8LA_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGS1812016.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(69387..69551)
                     /locus_tag="ACL8LA_000187"
     CDS             complement(69387..69551)
                     /locus_tag="ACL8LA_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS1812017.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            69994..71475
                     /gene="mgaSpn"
                     /locus_tag="ACL8LA_000188"
                     /gene_synonym="mgrA"
     CDS             69994..71475
                     /gene="mgaSpn"
                     /locus_tag="ACL8LA_000188"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGS1812018.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            71837..72838
                     /locus_tag="ACL8LA_000189"
     CDS             71837..72838
                     /locus_tag="ACL8LA_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1812019.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     KVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            72989..73906
                     /locus_tag="ACL8LA_000190"
     CDS             72989..73906
                     /locus_tag="ACL8LA_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812020.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAVRLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            73917..74807
                     /locus_tag="ACL8LA_000191"
     CDS             73917..74807
                     /locus_tag="ACL8LA_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812021.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            74836..76452
                     /locus_tag="ACL8LA_000192"
     CDS             74836..76452
                     /locus_tag="ACL8LA_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812022.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            76462..77781
                     /locus_tag="ACL8LA_000193"
     CDS             76462..77781
                     /locus_tag="ACL8LA_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS1812023.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            78292..78672
                     /locus_tag="ACL8LA_000194"
     CDS             78292..78672
                     /locus_tag="ACL8LA_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812024.1"
                     /translation="MRIQMRLSKEARVVLEELKLEYLKKGEDKTSGVIVDEIFKHFSS
                     NFQKVDWKSVQSAPKFNEILADYTSVNPTTLNLSNSTISTMSYIRDHFNYILDMKRTV
                     YRSFIIRLVLKAYKLEKEGTDIYL"
     gene            78785..79027
                     /locus_tag="ACL8LA_000195"
     CDS             78785..79027
                     /locus_tag="ACL8LA_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812025.1"
                     /translation="MKINEYLWRTSCLILLIIMSFFAGFGAKLEFTSYNPFLSVKFGL
                     VVALTVFVVQLLAILPAMVKRGNKNENVESNNSCEE"
     gene            78990..80381
                     /locus_tag="ACL8LA_000196"
     CDS             78990..80381
                     /locus_tag="ACL8LA_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HGS1812026.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            80637..81056
                     /locus_tag="ACL8LA_000197"
     CDS             80637..81056
                     /locus_tag="ACL8LA_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812027.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            81061..82080
                     /locus_tag="ACL8LA_000198"
     CDS             81061..82080
                     /locus_tag="ACL8LA_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HGS1812028.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            82084..82266
                     /locus_tag="ACL8LA_000199"
     CDS             82084..82266
                     /locus_tag="ACL8LA_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HGS1812029.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            82268..83395
                     /locus_tag="ACL8LA_000200"
     CDS             82268..83395
                     /locus_tag="ACL8LA_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1812030.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(83478..83550)
                     /locus_tag="ACL8LA_000201"
     tRNA            complement(83478..83550)
                     /locus_tag="ACL8LA_000201"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(83515..83517),aa:Lys,seq:ctt)
     gene            complement(83815..85086)
                     /locus_tag="ACL8LA_000202"
     CDS             complement(83815..85086)
                     /locus_tag="ACL8LA_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGS1812031.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            85356..85556
                     /locus_tag="ACL8LA_000203"
     CDS             85356..85556
                     /locus_tag="ACL8LA_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812032.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            85760..85939
                     /locus_tag="ACL8LA_000204"
     CDS             85760..85939
                     /locus_tag="ACL8LA_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812033.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            86076..86255
                     /locus_tag="ACL8LA_000205"
     CDS             86076..86255
                     /locus_tag="ACL8LA_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGS1812034.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            86292..86744
                     /locus_tag="ACL8LA_000206"
     CDS             86292..86744
                     /locus_tag="ACL8LA_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGS1812035.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            87035..87505
                     /locus_tag="ACL8LA_000207"
     CDS             87035..87505
                     /locus_tag="ACL8LA_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGS1812036.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            87524..88612
                     /locus_tag="ACL8LA_000208"
     CDS             87524..88612
                     /locus_tag="ACL8LA_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGS1812037.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            88593..89021
                     /locus_tag="ACL8LA_000209"
     CDS             88593..89021
                     /locus_tag="ACL8LA_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1812038.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            89159..90109
                     /gene="prmA"
                     /locus_tag="ACL8LA_000210"
     CDS             89159..90109
                     /gene="prmA"
                     /locus_tag="ACL8LA_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGS1812039.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            90111..90854
                     /locus_tag="ACL8LA_000211"
     CDS             90111..90854
                     /locus_tag="ACL8LA_000211"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGS1812040.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            90865..92661
                     /gene="pepF"
                     /locus_tag="ACL8LA_000212"
     CDS             90865..92661
                     /gene="pepF"
                     /locus_tag="ACL8LA_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS1812041.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(92721..93398)
                     /locus_tag="ACL8LA_000213"
     CDS             complement(92721..93398)
                     /locus_tag="ACL8LA_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGS1812042.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQKKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEQGKEF
                     LEKEWGI"
     gene            93645..94415
                     /locus_tag="ACL8LA_000214"
     CDS             93645..94415
                     /locus_tag="ACL8LA_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812043.1"
                     /translation="MLYLLGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDFLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
     gene            94408..95172
                     /locus_tag="ACL8LA_000215"
     CDS             94408..95172
                     /locus_tag="ACL8LA_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812044.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            95597..96388
                     /locus_tag="ACL8LA_000216"
     CDS             95597..96388
                     /locus_tag="ACL8LA_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGS1812045.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            96499..97167
                     /locus_tag="ACL8LA_000217"
     CDS             96499..97167
                     /locus_tag="ACL8LA_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS1812046.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFANKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLIIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT        30078 a        16319 c        20976 g        29927 t
ORIGIN      
        1 attgagtttg aagtggctta tttcaaagct ttttgtatgt cttcaatcat gagttttgtt
       61 gattcaagtc cgcctccgct tagataccag aggtctggtg ttagttggat aatcttacca
      121 tttttagcag caggtgtttc agcgataagg gcattttcta ggacaccgtc gttgctagag
      181 ttgtccccac cgatggcaag ggtacggttg atgacaaaga ggatgtcagg gttgatttct
      241 ttgatacttt caaagctgac ttcttgtccg tggcgtgagt cttcaaattt tgtatcagtt
      301 ggtttgaatt tcaaggtttg gtacaagaaa gagaaacgag atttggcacc aaaggctgcc
      361 atttttcctt cattaaggag gatcgcaagg gcttttttgt cagagctttc atttttagta
      421 gcgacttctt ggatgctctt gtctagcttg gccaattctt ccttggcttt ctgtgtacca
      481 gtttcgccga aggcacttgc taaggattcg atattagcct tggtagaagt ccagtagtcg
      541 tccttgcttg cttggaagag aacggttggg gcgatttctt tgaatttgtc tacgaatttt
      601 tgtgtacgtg gtgaagcgat aatcaaatca ggctcaaggg cggcgatagc ttctaaatca
      661 ggttctttca tagaaccaac atttttgaca gttcccacta ggtcttttag ataagtcgga
      721 acagtttttg taggcattcc gacgatattt ttttcaaatc ctaaagcgcg aatagtatcc
      781 gcagcgccga ggtcaaaggt cacaatcttt tcaggaactt tggaaagttt gacctcggcc
      841 agtgaacttt taatggttac ctctgttgga gcagagctac tggtctctgt ctgactagtg
      901 cttgagtttg tactacatgc accaagtagg agcaagaagc tggccactag ggcagtgaaa
      961 taaagtttaa gggatgtttt cataatttct cctctttaaa atgtgataac gatttaggga
     1021 gtctcttgat cttattgact aagagactga aggttctcta acttgagctt ttatgttact
     1081 agctatagat acagatcttt ttgtcattga tatcagctag cgtgatggga atctcataaa
     1141 gttgactgag caggtcagcc tgcatgattt gatcggttct tcccttgcta aagacctggc
     1201 cgtccttgaa ggcgacaatt tcatctgcat actgactggc tatgttgata tcgtggagga
     1261 cgatgataat ggtcttgccg agttcctcca ccagtcgtcg aagaatctgc atcatgctga
     1321 cgctttgctt gatatcgaga ttgttgagtg gttcgtccag caagataaag tccgtatcct
     1381 gggccagtac catagcgata aagacgcgct ggagttgccc ccctgacagg ctattgatgt
     1441 agcggtcttt taagttggtc agttccaaat agttcagagt ttctcggatt ttttcccagt
     1501 cttctgatct aagtcgacct cggctgtagg gaaaacgtcc aaaactgacc agttcttcaa
     1561 cagtcaattt ggcttggtaa ttgattttct gttttaggat ggttagttct tgggccagtt
     1621 cttgcgaatt ccagctctcg atttcacgtc ctttgatact gagaactccc tgatctttct
     1681 tggttagtct gctcatgatg gagaggagag tcgattttcc agcaccattt ggaccaataa
     1741 aggctgtcag tttttgagga ctgacttcaa gcgaaatgcc ttgcaaaata tcctgttttt
     1801 gaatggattt gtcaatgttt tccagtttca ctgacgagcc ctcctatata gtaagataaa
     1861 gaataagaag ccacccacac tctcaatgat catactgata cgaatttcca gtgcaaagac
     1921 tcgttcaatc aaggcttgcc ccaaggttaa gctaataaat ccaaccagaa tggccactat
     1981 aaagagtaac ttgtgctgat agtctttgac aatcaggtag gtgaggttgg ccagcataaa
     2041 gccgaagaag gccataggtc ctaccaaggc agtggccgtt gaggtcaaaa gcacgattcc
     2101 ccagaggagc tctttctgtt ctttttcaac atcgagtccc aatatctgag ccgtttctct
     2161 ttgcaggtgc aagacatcta gaacgactgc ttttcgaaag aaaaagattg tcaaagcgag
     2221 gatgatcaga gaaccgatgg ctaggatgga agtgttgaga tgttgaaagg aggcaaaaag
     2281 actattttgc agtttatcgt attcgtttgg atccattagg acttgaagga aggtgctgat
     2341 atttcgaaag agacttctga gcgctagaca gatcagcagg atgaagacca ggtcttgctt
     2401 catcagtgtc ttcaagtaac cttgtaaggc gagaaagaag agggactgga caagaagtaa
     2461 gactaggaat tctaagatag gggatttgcc aagttgaaga aacttgcttt caaaaaccag
     2521 tagtagggtt tgtagtagga cgtagaagga ttcaattccc aaaatactag gcgtcaggaa
     2581 gcgattttcc gtcagggttt gaaaactaat ggtcgaaatc ccagtcgcga tggctaccaa
     2641 gagataaacg atgatctttt gggaacgcaa cttccaagca aaggctgaca agtgagtgat
     2701 gggccaaaag tagagaagac aagctccgat ggcaagaata atgagaatcc agaagagctt
     2761 ggtatgtttg cttttagtct gcatcttttc gtccccctct ccagagaagt aggataaaga
     2821 cgagactacc gatgattcct agcaagagac tgacagacaa ctcatagggc ctaatcagaa
     2881 ctcgggatag gatatcgcaa gccagaacta gattggcacc aaccagtgcg accatgagtt
     2941 tggtttgact tagattatct ccatagcgct tgcgaacaag attgggaacg ataactccga
     3001 gaaatggtag gccacccacg gtaatcatgg tgacgcttgt cgttagcgcc accagaaaga
     3061 gggccagttt ttcaagtagg gagtaggaaa tccccaaact ctcgctggtt tctttcccta
     3121 gattcatgat ggtgaaggtt tgggataatt tccaaacggt tatcaggatg atgaggccta
     3181 agaagagcca ctcatactga tgggtctgaa tcatggagaa ggagccctgg gtccaggcag
     3241 tcatactctg aaccagattg aaacgatagg cgataacttc tgtgactgag ccgataatcc
     3301 cgctatagat gatcccaatc agaggcaaca tccacctttc ctttacagta aaaatggtca
     3361 taaaggctag gaagaagagg gtgaatacga tggatgaaac aaaagcgaag agcatctttt
     3421 gggtcagact agccgatgga aagacaaaaa ggctcagcac cattcccagt ttggcggctt
     3481 cagtcgttcc aactgtactc ggtgcagcaa actgattttg ggtaatagtc tgcatgagaa
     3541 ggcctgccat actcatacta gaggcagtca ggagaatact gatagttctt gggagacggg
     3601 actcttgaaa gaggagccag gtctgctggt cgaaatcaaa tagctttccc catgaaaaat
     3661 cactggtccc aatgctaata gagagaaaga ctaggagtag aagtaagcca attaaataat
     3721 gagaaagttt cataccccgt cctttcatgt agatttggta tcgaaagata tctgcggata
     3781 taaatgtaac attatttttc taatctgtca ataaaatttc tgacaattta ataaatacaa
     3841 caaggagaga gcaacaagac tttctccttt gttatcctat tctaaaatgt ttttacctta
     3901 atctgataaa ataatatctt cgagggagta gctagccgtc caatcaagat attgtttagc
     3961 ttttgaagca tctgctagga cactggctgg gtcactagca cgtcgagcaa caatctcgtg
     4021 tgggattttt taatttagta attcttcagc agttttaaag atttctttga tagtatagcc
     4081 ttttttagtt cctaagttaa agatttgaga agaactgtct tcttgaaata ggtagttcat
     4141 tcctttaaca tgagcctatg caaggtccaa gacataaatg taatctcgaa tacatgaacc
     4201 gtcacgtgta tcgtagtcat ctccaaatat ttttaagcta tcattttgtc ccaatgcggt
     4261 cttgttgata tttggaatga tgtgagttgg atttttcaca cgcagaccgt ttgaagcatc
     4321 catttcagcc ccagcaacat taaagtaacg gaaaataaca tatttccagt cgtagcgatt
     4381 ggccatccag taaatcattc gttcgcccat cagttttgtc tctgcataag ggttgacagg
     4441 gtcgagcagg gtatcttcag tcaccggctt gtcaatacag ttatttccat agagagaagc
     4501 agtcgaagag aacatgattt tttgaatgcc aacttcagat aagactttga gaacttggtt
     4561 cataccagca acgttggcag tgaagtattt acttggattt tcaatacttt cgctcacaac
     4621 aatttcacct gcacaatgga ggacagcatc aatctgattt tcttctaagt aagcctttaa
     4681 ggcgctagca tcataaacat ccagttcttt aaagctagca cgactatcca cagccgctcg
     4741 atttcctgtg gagagattat cgaggacatg caccttatag ccagcattta aaagagcttt
     4801 aacggtatgg gagccaatgt agctagtccc acctgtaaca agaattgttt tggtcatgat
     4861 tttttccttt tctagttttg tactatagta aattgaaata agatatgaac aactctatta
     4921 ggaaagtcaa attaatttct agaaatgttt cagcagctac agcgtactat tccaaactca
     4981 accaactata tatttaaaga aagaagaaag ggaagtcaac tattttctgt aaatttcatg
     5041 aaaaatccaa acaactttga tggtgtattg tttttttgtt tggaaataaa tattcgagtt
     5101 aattttggaa tgtttactag attttgcact acatgcaaga gaatactatc cttttctaag
     5161 atagagcagg actaagatac taacttagaa atccaccttc ccactatcct tctcctataa
     5221 aaagtggtaa aatggatgaa agaaagaagg aactgaaatg acaacattat tttcaaaaat
     5281 taaagaagta acagaacttg ctgcagtctc aggtcatgaa gcgcctgtcc gtgcttatct
     5341 tcgtgaaaag ttgacaccgc atgtggatga agtggtgaca gatggcttgg gtggtatttt
     5401 tggtatcaaa cattcagaag ctgtggatgc accgcgcgtc ttggtcgctt ctcatatgga
     5461 cgaagttggt tttatggtca gcgaaatcaa gccagatggt accttccgtg tcgtagaaat
     5521 cggtggctgg aaccccatgg tggttagcag ccaacgtttc aaactcttga ctcgtgatgg
     5581 tcatgaaatt cctgtgattt caggttctgt tcctccgcat ttgactcgtg gaaagggggg
     5641 accaaccatg ccagccattg ccgatatcgt ttttgatggt ggttttgcgg acaaggctga
     5701 ggcagaaagt tttggcatcc gtcctggtga taccatcgta ccagatagtt ctgcaatttt
     5761 gacagccaat gaaaaaaata tcatctcaaa agcttgggat aaccgctacg gtgtcctcat
     5821 ggtaagcgag ctagctgaag ctttatcagg tcaaaaactc ggcaatgaac tctatctggg
     5881 ttctaacgtc caagaagaag ttggtctgcg tggcgctcat acctctacaa ccaagtttga
     5941 cccagaagtc ttcctcgcag ttgattgctc accagcaggt gatgtctacg gtggtcaagg
     6001 caagattgga gatggaacct tgattcgttt ctatgatcca ggtcacttgc ttctcccagg
     6061 gatgaaggat ttccttttga caacggctga agaagctggt atcaagtacc aatactactg
     6121 tggtaaaggc ggaacagatg caggtgcagc tcatctgaaa aatggtggtg tcccatcaac
     6181 aactatcggt gtctgcgctc gttatatcca ttctcaccaa accctctatg caatggatga
     6241 cttcctagaa gcgcaagctt tcttacaagc cttggtgaag aaattggatc gttcaacggt
     6301 tgatttgatt aaacattatt aaaccataag ggaggtggta gggatggtag aaccaaacct
     6361 agaaagcctt ataaaagatc tttacaatca tgctcgacat gatttgagtg aagatttagt
     6421 tgctgctctc ctagagacta ctaaaaaact gcctactaca aatgagcaat tgcaggcagt
     6481 tcgtctctca agcctggtca atcgtgaatt gctcctaaat cccaaacatc cagcacctga
     6541 gttgctcaac ttggctcgct ttgtcaaaag agaagaagcc aagtacagag gaactgcgac
     6601 ttctgcgctt atgtatgagg aactttttaa aatgctttga ttccattgtg gatcaaagca
     6661 ttttttataa gacctatctc gtgagcgttc aaatagtagt aacaaggaat catgagaagt
     6721 cagccgagac catagtagtg aagaaacttc tgtaatggaa gtggagtgaa gtgaggaatc
     6781 atcaaacggt aggggatagg ctgtttgaag ggtgctacga aaaagatagg gtaaggtgaa
     6841 attatagcgg aatgaaataa gatatgaaca aattgattag ggaaatcaaa tgaatttata
     6901 gaaatctttt agcagtcatg gcgtcctatt ctagtttcaa aatgctataa atacgaacaa
     6961 attgattggg aaattcaaat cgctttctat caatgtttta gaagatacag tgccctagtc
     7021 caacttcaat atactataaa agaacagatg gaagcaatta tgatttactt ctatctttaa
     7081 aataaatgat ctgataggaa aaatgtgtcc cctcataaaa aataggatag tacggatgga
     7141 aggcttttgt agactggagg acgaaggaat ttagaaactc tcgttaaaag ttagttgagc
     7201 cggagtatgg tggtgtgaat aattatcaaa aattatttcc aaatcgaaat agttgacagt
     7261 tagaacgatg agtgttacaa ttattttatt catttcgata tcgaaataaa ttggaggtgt
     7321 tatgaaagat agtcatttgc tagcccatca tattcgtttg ttgaatgggc ggatttttca
     7381 aaagttactg agtcaagatc ctgaggctct ttataggggt gaacagggca agattttagc
     7441 ggttttatgg aatagtgaaa ctggctgcgc aactgcgaca gatatcgcgc ttgcgactgg
     7501 gcttgcgaat aatacgctga cgactatgat aaaaaagcta gaggaacaaa agcttgtaat
     7561 tgttagtccg tgtggaaaag acaagcgtaa gaagtattta gttttaacgg agttaggcaa
     7621 gttccagaaa gaagtggggc atcgtgtcag tcagaaattg gatactatct tttacaaagg
     7681 attttcagag gaagaaattc accaatttga aggttttcaa gaaagaattt tggcaaatct
     7741 gaaagagaag gaaaatgagg tttagaatat gtcacaacaa gtgaaaaatg ctcacaacct
     7801 atacatccat gccattcaag acggacgagt tgctgaggct caagcccagt ctgtagggga
     7861 tacctatatc caacattcga caggtgttcc tgacgggaaa gaaggatttg cagccttctt
     7921 tgcagatttc tttgagcgtc atccagagcg tcagattaag attgtccgca ccattgagga
     7981 tggcaatctg gtctttgttc atgttcatca atatctgaat ggtggagaag ctcaatgggt
     8041 gacgacggat actttccgtg cggatgagaa tggtcgtatt gttgagcatt gggatgtcat
     8101 tgactactat cgaacacctg aaaatgacca actagatcaa atttttggag attttgaaat
     8161 caaggatttg gacaagaaag cagaaaataa aaagctggtt cgccgtttct tgacagaaac
     8221 tttccaaaat ggggagctag agcagtggag tgattatgtg gcagacgatt tgattcagca
     8281 taatcatgag attggacaag gaagtgctgc ttataaaaac tatgtggctg aatatagtgt
     8341 cacttttgac tttgttttcc aactcttagg acaaggaaac tatgtggtta gctatggtca
     8401 gactcagatt gatggcgttg cttatgccca gtacgatatc ttccgtttag agaacgggaa
     8461 aattgtggag cattgggata ataaggaagt catacctaag gtagaagact tgactaatcg
     8521 agggaagttt taaattgagg acaaagaatg attgaataca aaaatgtagc actgcgctac
     8581 acagaaaaag atgtcttgag agatgtcaat ttacagattg agaatgggga gttcatggtt
     8641 ttagttgggc cttctgggtc aggtaagacg accatgatta agatgattaa ccgtctcttg
     8701 gaaccaactg atggaaatat ttatatggat ggcaagcgca tcaaagacta tgatgagcgt
     8761 gaacttcgtc ttactactgg atatgtttta caggctattg ctctctttcc caatctaaca
     8821 gttgaggaaa atattgctct cattcctgaa atgaagggtt ggactaagga agaaattgct
     8881 caaaaaacag aagagctttt ggcaaaagtt ggattaccag tagctgagta tgggcatcga
     8941 cttccaagtg agctatctgg tggagaacag caacgggtcg gtattgtccg agctatgatt
     9001 ggtcagccca agattttcct catggatgaa cccttttcgg ccttggatgc tatttcgaga
     9061 aaacagttgc aggttctgac aaaagaattg cataaagagt ttgggatgac aacgattttt
     9121 gtaacccatg atacggatga agccttgaag ttggcggacc gtattgctgt cttgcaggat
     9181 ggagaaattc gccaggtagc gaatcccgag acaattttaa aagtgcctgc aacagacttt
     9241 gtagcagact tgtttggagg tagtgttcat gactaattta attgcaactt ttcaggatcg
     9301 ttttagtgat tggttgacag ccctatctca acatttgcag ttgtcacttt tgaccttgtt
     9361 actagctatt ttgcttgcga ttcccttggc tgtttatctt cgctatcatg agaagttggc
     9421 ggattgggtc ttacagattg cagggatttt ccagaccatc ccgtctctgg ccttgttggg
     9481 actctttatt cccttgatgg gaattgggac tttaccggca ttgacagctc tagtgattta
     9541 tgcgattttt ccgattttac aaaatactat tactgggctg aagggaattg atccgagtct
     9601 tcaagaggct gggattgcct ttgggatgac cagatgggag cgtctcaaga agtttgaaat
     9661 tccacttgcc atgcctgtta tcatgtctgg aattcggacg gcagcggtct tgattatcgg
     9721 tacggctacc ttggcgacct tgattggggc agggggactg ggttccttta tcctgttggg
     9781 aattgaccgt aataatgcca gtttgatttt gattggggcc ctttcttctg cagtgctagc
     9841 tattgccttt aacttcctac taaaagtaat ggaaaaagcg aaattgcgga cgattttctc
     9901 tggttttgcc ttggtggctt tattactggg tctgtcttat agtccagccc ttttggctca
     9961 aaaagagaag gaaaacttga ttattgctgg gaaaataggc ccagaaccag aaattttggc
    10021 taatatgtat aaattgctga ttgaagaaaa taccagcatg actgcgactg ttaaaccgaa
    10081 ttttgggacg acaagtttcc tttatgaagc tctgaaaaaa ggtgatattg acatttatcc
    10141 tgaatttacc ggtacggtga ctgaaagttt gcttcaacca tcacccaagg tgagtcatga
    10201 gccagagcag gtttatcaag tggcgcgtga tggcattgcc aagcaggatc atctagccta
    10261 tctcaaacct atgtcttatc aaaataccta tgctgtagct gttccgaaaa agattgctca
    10321 agaatatggc ttgaagacca tttcggattt gaaaaaagtg gaagggcagc tgaaggcagg
    10381 ctttacgctt gagtttaacg accgtgaaga tggaaataag ggcttgcaat caatgtatgg
    10441 tctcaatctc aatgtggcga ctatgcagcc agctcttcgt tatcaggcaa ttcagtcagg
    10501 ggatattcaa atcacggatg cctattcgac tgatgcggaa ttggagcgtt atgatttaca
    10561 ggtcttggaa gatgacaagc aactcttccc accttatcaa ggggctccac tcatgaaaga
    10621 agctcttctc aagaaacacc cagagttgga aagagttctt aatacattgg ctggtaagat
    10681 tacagaaagc cagatgagcc agctcaacta ccaagtcggt gttgaaggca agtcagcaaa
    10741 gcaagtagcc aaggagtttc tccaagaaca aggtttgttg aagaaatgat gggacagaga
    10801 ctttaaacat actgaactca acttccttaa acgactagat aaaagaatgc tcagacaatt
    10861 tcgtctgggc ttttttgatg tgagaatgac tgaatgtcac ttaaaatgga aagaaagaga
    10921 aaattttcat gattttctaa aattttactg taaagacagt tgactattca taaaataggt
    10981 gtataatatt ttgtgaacta cttaacaaat aagaggtttc agctcatgtc gactaaggtt
    11041 agaaatcttg tgtataggca gttcattatc taatagagcg ttgcgtccga aagtctatcc
    11101 agacacggct ctttaaaaac aaaaggagaa atgatgcata cttatttgca aaagaaaatt
    11161 gaaaatatca aaacaaccct aggtgaaatg tcaggtggtt accgtcgtat ggttgcggct
    11221 atggctgatt taggattttc aggaactatg aaggctatct gggatgacct ctttgcccat
    11281 cgtagttttg cccagtggat ttatttgctg gttttaggaa gttttcctct ctggctggag
    11341 ttggtttacg aacatcgtat tgttgactgg attgggatga tttgtagctt gacagggatt
    11401 atctgtgtaa tctttgtatc ggaaggtcga gcaagtaatt atctttttgg cttgattaac
    11461 tctgttattt accttatttt ggccctacag aaaggctttt atggtgaggt gctgacgaca
    11521 ctttacttca cagtcatgca gccaattgga cttctagttt ggatttatca ggcacagttt
    11581 aagaaggaaa agcaggagtt tgtcgcgcgt aaactggacg gcaagggctg gacaaagtat
    11641 ctttccatta gtgtgctttg gtggttggcc tttggcttca tttatcagtc tattggtgcc
    11701 aatcgtccct atcgtgattc aatcacagat gcaaccaatg gggtagggca aatcctcatg
    11761 acagctgttt accgtgaaca gtggatattc tgggcggcta ccaatgtttt ttcaatctat
    11821 ctctggtggg gagaaagcct gcaaattcaa gggaaatatc taatttatct cattaacagt
    11881 ctagttggtt ggtatcaatg gagcaaggca gctaagcaga atactgattt acttaactag
    11941 gaaaagatgt ttgaaagtgc tgttttgaga tttcgattaa aacagatata gttgataatc
    12001 aaggatttat agtatgaaaa agaggatcgg cgggtcctct tttgttgttg aaaagataaa
    12061 aaactcagta acctagaaat aagacaactg agctttactc tatattcaat ttttaggaat
    12121 gagaaggtct agataaaatt ggacaacttc ctggcctgtg aaatcttgac cttttttgag
    12181 ccaccaggtc aatgtctcga taaagttgga cacgaccaag tgttggaggt aagaagtagg
    12241 catattaggg tgggcttctt ttaaattatc agctagcacg gaatagacat ggtgttctag
    12301 ctctttatgg agttgacgga ggaagtagtc atttttggaa aatagcagac tggtgatatg
    12361 gtcttggttt ttatgaaaat ggagaaagag gtgggcgagg tagtcctcgg ttgaaatggc
    12421 ttgctctctt tcaaaaagat gatggaagag gtagcggcag agctggtcca gaagaagctc
    12481 cttactttca tagtgacagt aaaaggtgga tcttcccaca tctgcgagat caatgatatc
    12541 ctgaacagta gtggcctcgt agcccttagc attcaaaagt tgtataaaag cttgatagat
    12601 ggcttttttg gttttgctga tacggcggtc aatgttagtc atatggacac ttaaggcaaa
    12661 ttgttcagaa ctgaataaag ctgacgtttt gcttctatcc tttctttgag ttttagtgga
    12721 taatgataat gaacaaggtg ttcataaatc tattataaca aaggaatgag aaatatgaag
    12781 gcaaaatatg ctgtttgggt ggcttttttc ttaaatttga cttatgccat tgttgagttt
    12841 attgcaggtg gagtatttgg ttctagcgct gttcttgctg actctgtgca tgacttggga
    12901 gatgcgattg caattggaat atcagctttt ctagaaacaa tctccaatcg tgaagaagac
    12961 aatcagtaca ccttgggcta taagcggttt agcctgctag gagccttggt aacagctgtg
    13021 attctcgtaa cgggctctgt tctagtcatt ttggaaaatg tcacgaagat tttgcatccg
    13081 caaccagtca atgatgaggg gattctctgg ttaggaatta ttgcgattac tatcaatctg
    13141 ttagcgagtc tggtggttgg taagggaaag acaaagaatg agtctattct gagtttgcat
    13201 tttctggaag atacgctagg gtgggtagct gttatcctga tggcgattgt tcttcgattt
    13261 acggactggt atatcctaga tcctcttttg tcccttgtca tttctttctt tattctttca
    13321 aaagcccttc cacgtttttg gtctacactc aagattttct tggatgctgt gccagaaggt
    13381 cttgatatca agcaagtaaa gagtggcctg gagcgattgg acaatgtggc cagccttaat
    13441 cagcttaatc tctggactat ggatgctttg gaaaaaaatg ccattgtcca tgtttgtcta
    13501 aaagaaatgg aacatatgga aacttgtaaa gagtctattc gaattttcct aaaagattgt
    13561 ggttttcaaa atattaccat tgaaattgat gctgacctag aaactcacca aacccataag
    13621 cgaaaggtgt gtgacttgga acggagttat gagcatcaac attagaaaaa agtgaaaaat
    13681 acttgggtac tatcttattt ggaatagagt aatttcttta ttatttaaat atttcaaaaa
    13741 ttggtaagag aagagcattg tataaactcc ggatatatga ttgttaatga taaaaatttt
    13801 tcgattagat acaaaatgct tgacttggag tcaactcaaa gttatataat aagataagtg
    13861 agttagaata gcgtgaattc agtgaatgaa atgagaggag gttagcgtgt gaatattaaa
    13921 tctgccagtg atttgttggg aatttcagcg gatacgattc ggtattatga acgggttggt
    13981 cttgtgccac cgattactcg tactgctact gggattcgtg attttcaaga tcaggatatc
    14041 gaagcgctgg aatttattaa gtgttttcgt tcggcgggtg tctctgtaga tagtttagtt
    14101 gactatatgt cgctctacca aaagggagat gaaacgagag aggagaggct tggtatttta
    14161 gaagaggaaa agcaaaaatt agaggagcgc ttgtctcagc tacagacagc tttaaatcgt
    14221 ttaaatctca aaattaaact ttataaggaa ggaaaatttt aaatgaaatc agcagtatat
    14281 acaaaggcag gtcaggttgg acttgctagc attgaacgtc cgcaaataat agaagcggat
    14341 gatgtgatta ttcgtgtggt tcgtgcgtgc gtttgtggtt cagatttatg gaggtaccgt
    14401 aatccagaaa cgaaagctgg acataaaaat agtggacacg aagcgattgg gattgttgaa
    14461 gaagctgggg aagccattac gacggtgaaa ccaggtgatt ttgtgattgt cccttttaca
    14521 catggatgtg gtgagtgtga tgcctgtctt gctggatttg acggttcttg cgacaatcat
    14581 attggcaata atttgggggg tgattttcag gcagaatata ttcgcttcca ctatgcaaac
    14641 tgggcgctgg ttaaaatccc tggtcaacct tctgattata cagaagggat gctcaagtcc
    14701 cttttgactc ttgcagatgt catgccgaca ggctatcatg cagcgcgtgt tgcaaatgtt
    14761 caaaaagggg acaaggttgt tgttatcggt gatggggctg ttggtcaatg tgctgtcatc
    14821 gcggctaaga tgcgtggagc atcacaaatt atccttatga gccgtcatga agaccgtcaa
    14881 aagatggcta tggagtcagg tgcgacagct gttgttgcag aacgtggtca agaaggaatt
    14941 accaaggtgc gtgaaatcct cggtggagga gcagatgcag cacttgaatg tgttggtacg
    15001 gaggctgcta tagaacaggc gctaggtgtt cttcataatg gagggcgtat gggctttgta
    15061 ggagtcccac actataataa tcgtgctctt ggttcgacat ttatgcaaaa tatctctgta
    15121 gcaggtgggg cagcttctgc tacaacatac gataagcaat ttttactaaa agccgtcctt
    15181 gatggtgata tcaatccagg tcgcgtcttt acttcaagtt ataaactgga agatatcgac
    15241 caagcctaca aagatatgga tgaacgtaag accattaagt ctatgattat aatcgaataa
    15301 aaaacgaata ggagttctaa aactcctatt cgttttttat gttatcctat tcttgattta
    15361 gggtactttc tcttaatgtc agtctggttc ccagcatggt caggctaggg attttccgac
    15421 cgtggaggac ttccttgtta agaatatcca tacctgctcg gcccatttct tcagtataaa
    15481 ctgtaatact agagagggga ggatagacct gtttggtcag actagtgtcg ttaaaggaaa
    15541 tgaggctgac gcgatctggc aggttgattc cagcttcttg gagggcacgg agggcaccga
    15601 tagctaaact atcgctggct gcgaaaaatg ctggcggaag ttggtctccc aagctctgaa
    15661 tggcctcctt catcaagtca tagccagact gggcagtaaa gtttccttga aagatcagtt
    15721 catcatgata gattcctttt gcttgactgt agtttctgaa attttccagc cgcttatcct
    15781 caatgatttc ttcttggtct gttgtttctt caaggcctgt tagaatcccg atacggtcca
    15841 ttccttgact gaggaaataa tcgacaacct gtttcatagc agtgtaaaaa tccgtgataa
    15901 tacaggtatg tcccagggaa agtgtatcgc tgtctagaaa tacaagaggc ttttggtatt
    15961 cttcaaaggc agaaatctga gctcgactaa actttccgat gcagagaatc ccaatcactt
    16021 cctcgcttag ggtaaaaggg tggtcattaa aatagcgcaa gatatcgtag tccaactctt
    16081 gggctctttt ttctattcct aggcgaatct ggtagtagta gaggtcgtct agctcccctt
    16141 gttcgctgac ccattgaata atggcaatct tttgcttggg tttgtgggac tcgcctgtct
    16201 tgaggtgctt ggtgtagccc agctcttcag caacggttaa aatacggtgt ctggtttctt
    16261 ctgtaacaga taggctctgg tcgcggttga ggacacggga tacggtcgcg attgagacag
    16321 aggctagctg tgcaatgtct tttaaggtag ccataaatcc tccttgatta ggttagtata
    16381 tcatattttt cttcttttta ctgatatttt agtaaaattt tagtaaaaag gattgacctt
    16441 gggaaattcc ttggatacaa tagaaagaaa acgattacac gttaagatgg cttaacggac
    16501 agtcaaagga gaattcatat ggcacaacat cttactactg aagtccttcg caaagacttt
    16561 cttgctgttt ttggtcaaga agcagaccaa accttctttt caccaggccg tattaatttg
    16621 attggtgaac acacagacta caacggtggg cacgtttttc ctgctgctat ttccttggga
    16681 acttacggtg cagctcgtaa gcgtgacgac caagtcttgc gtttctactc agctaacttt
    16741 gaggacaagg gcattatcga agtgcctctc gctgacctca agtttgaaaa agagcacaac
    16801 tggaccaatt atccaaaagg ggttcttcat ttcttgcaag aagctgggca cgtgattgac
    16861 aaaggttttg atttttatgt ttatgggaat atcccaaatg gtgctggctt gtcttcttca
    16921 gcatccttgg agctcttgac aggagtcgtg gcagagcatc tctttgattt aaaactagac
    16981 cgtttggatt tggttaaaat cggaaaacaa acagaaaaca actttatcgg agtcaactct
    17041 ggtatcatgg accagtttgc tattggtatg ggggctgacc aacgtgctat ttacttagat
    17101 accaatactt tagaatacga cttggtgcca cttgatttga aggacaatgt cgttgttatc
    17161 atgaacacca acaaacgccg tgaattggcg gactctaaat acaatgaacg tcgtgctgag
    17221 tgtgaaaaag cagtggaaga attgcaagtt gccttggata ttcagaccct gggtgaattg
    17281 gacgagtggg ccgttgacca atatagctat ttgattaaag atgaaaatcg tttgaaacgt
    17341 gctcgccatg ctgtgcttga aaaccaacgt acccttaaag ctcaagcagc ccttcaagca
    17401 ggtgatttgg aaacatttgg tcgtttgatg aatgcgtcac acgtttctct ggagcatgat
    17461 tatgaagtaa ctggtttgga attggatact cttgttcaca cagcttgggc acaagaagga
    17521 gttctcggtg ctcgtatgac aggggctggt tttggtggct gtgccattgc cttggttcaa
    17581 aaagatactg ttgaggcctt taaggaagct gtaggcaaac actacgagga agtagttgga
    17641 tacgctccaa gcttctatat cgctgaagtt gcaggtggca ctcgcgtcct tgactagtca
    17701 aaaggaggct ctatagtgat cttagtagat aaatttgtaa cacatgtcat ttctgaaagt
    17761 tcatttgagg aaatggatcg aatctacctg accaaccgtg ttttggcacg agtgggagaa
    17821 ggtgttttgg aagttgagac caatctggat aaattgattg acctcaagga ccagctagtt
    17881 gaggaagccg ttcgattaga gatgattgag gatagtcaga ctgcgcgtga aatccttggt
    17941 actgaactga tggacttggt gactccttat ccgagtcagg tcaatcgtga cttctgggaa
    18001 gcctatgtcc actctcctga gcaagcgata gaggattttt atcaactcag tcagaaaaat
    18061 gattacatca aactcaaggc cattgctaaa aatatcgctt atcgtgttcc atctgattat
    18121 ggagaacttg aaattaccat caatctctct aagcctgaaa aggatccgaa agagattgcg
    18181 gtagccaagt tggtgcaagc tagtaattat cctcagtgtc aactttgttt agagaatgag
    18241 ggttaccatg gtcgggtcaa ccacccagct cgcagcaacc accggattat ccgttttgaa
    18301 atggttggtc aggaatgggg gttccagtat tcgccctatg cttactttaa tgagcattgt
    18361 atctttttag atggccagca tcgtcccatg gccattagtc gtcagagttt tgaacgtctg
    18421 ttggctatcg tagagcagtt tccaggatat tttgctggat ctaatgccga cctgccgatt
    18481 gtggggggct ctattctaac tcatgatcat tatcagggag gccgtcacgt atttcctatg
    18541 gaattggctc ccttgcaaaa gaccttccga tttgctggtt ttgagcaggt caaggctgga
    18601 attatcaagt ggccaatgtc tgtcctacgc ttgacttcgg attccaaaga ggatttgatt
    18661 aacttagctg ataagatttt ccaggaatgg cgtcagtatt cagattctag tgtgcagatt
    18721 ttagcagaga cagacgggac accgcatcac accatcacac caatagcgcg taagcgtgat
    18781 ggacagtttg agttggactt ggtcttgcga gacaatcaga cttcagcaga gcatcctgat
    18841 ggcatctatc atccccacaa ggatgtccaa catatcaaga aggaaaatat cggcttgatt
    18901 gaggtcatgg gcttggcaat cttgccacca cgtctgaaag aagaagtaga gcaagtcgct
    18961 agctatcttg taggagaagc tgttacagtt gccgattatc atcaggagtg ggcagaccaa
    19021 ctcaaatccc aacatccaga cctaacggat aaagaaaaag cccttgcaat cgtcaaggac
    19081 tctgtgggtg ctatctttgt gcgtgtactt gaggatgcag gagtctacaa gcagacagaa
    19141 caagggcaga cagcctttat gcgctttgtg gaacaggtcg gaattttact agactaggag
    19201 ctttctcctt gcacagtcct ataaaaagta gtatcatagt gtcgtttgaa atatcaggag
    19261 gaaacgatga aagactttca ttttgacgct atatctgcct ttgaaagtta cgaaattgaa
    19321 aaaatgagag atggtcacgt tgtggtgacg accaaagtag tgaactcatc gctcaactac
    19381 tatggcaatg cccatggtgg ctatctattc acgctatgtg accaaatcag tggtttggtg
    19441 gttatctcgc tgggacttga tggggtgaca ctccaatcct ctatcaacta ccttaaggca
    19501 ggaaaactcg acgatgtatt gaccattaaa ggagaatgtg tccatcaagg tcgtacaacc
    19561 tgtgtgatgg atgtggatat caccaatcaa gagggcagaa atgtctgtaa ggcaaccttt
    19621 accatgtttg tcacaggcca aaggtctgag gaaagacggg taagtatata aagaaaggtg
    19681 agtgggataa atttatctca ctcattttta atttatgaga tatgttagaa ggaaatataa
    19741 aagagagtga tgaaatacaa atgtaatagg tacatatatg tggtatacta gttatagtag
    19801 attttaattg cttatatcga gtgagttctc atattagtga ggaattttta aatggaatta
    19861 cactttaatt tagaattagt agaaacctat aaaagtaata gccaaaaggc acgtatctta
    19921 acggaggatt gggtttatcg tcagtcttat tgccctaatt gtgggaacaa tcccttaaat
    19981 cattttgaaa ataatcggcc tgtagcagat ttttactgta atcattgtag tgaggagttt
    20041 gaactaaaga gcaaaaaagg aaatttttca tcaacaatca atgatggtgc ttatgcaacg
    20101 atgatgaagc gtgtgcaggc agataataat cctaatttct tttttttaac ttacacaaaa
    20161 aattttgagg taaataactt tcttgtcctt ccgaagcaat ttgttacacc gaaatcgatt
    20221 attcaaagaa aaccacttgc accaactgct agacgagcag gttggattgg ttgtaacatt
    20281 gatttatcac aagtaccttc taaaggaagg atatttcttg tgcaagatgg acaagttaga
    20341 gatccagaaa aagttacaaa agaatttaag caaggtttat ttttaaggaa gagctctctg
    20401 tcatcaagag gttggacaat agaaattcta aattgtatag ataagataga gggttcagaa
    20461 tttacccttg aagatatgta tcgttttgaa agtgacctaa aaaatatctt tgttaagaac
    20521 aatcatatca aagaaaagat taggcaacag cttcaaatat taagagacaa agaaataata
    20581 gaatttaaag gtagaggaaa gtatcggaaa ttatgaaaac gaaacaactt gttgcatcag
    20641 aagaggtgta tgatttctta aaagtcatct ggcctgatta tgaaactgaa agccgttacg
    20701 ataacctaag tttaatcgtc tgtaccttat cagatcccga ttgtgtgaga tggttatctg
    20761 aaaatatgaa atttggtgac gaaaaacaac tagctttgat gaaggaaaaa tatgggtggg
    20821 aagtaggaga taaattgcca gagtggctac atagctccta tcatagatta ttgttaatag
    20881 gtgaattatt ggaaagcaat ctaaaactga aaaagtatac agtagaaatt acagaaactt
    20941 tatcacgttt agtaagtata gaggctgaaa atccagatga agccgaacga cttgtaagag
    21001 aaaagtataa gagttgtgaa attgttcttg atgcagatga ttttcaggac tatgacacta
    21061 gcatatatga ataggtagat gtttttattt tgtcacacaa aaaagaggct cgcacctctt
    21121 tttcttattt ctttttatga tttaatacgg cattgaggac aatagcgagt aggctggcta
    21181 cgacgattcc gtttgagaag aacatttgga aggctgtcgg catgctgaca aagagattac
    21241 tgttgttgag accgacacct gcagcgattg aaacagctgc gataaggaag ttgtgttcat
    21301 tgttagcaaa gtcaactcgg gcgaggattt gcatcccttg aatagataca aaaccaaaca
    21361 tcaccagcat ggcaccgccg aggacggagc ttggaatgat ttgggcaagg gcaccaaact
    21421 taggaagcag tccaaggaga accaggaaac cagctgcgta gtagattggc aggcgttttt
    21481 tgatgcctga caatttaacc aaaccaacgt tttgtgaaaa tccggtgtaa gggaaggtgt
    21541 taaagattcc tccgagaagt accgccaaac cttctgcgcg gtaaccgttg cgaaggcgcg
    21601 tgctgtcgat tggatctttt gtgatatcag acaaggctag ataaacacca gttgactcaa
    21661 ccatagacac cgttgcgatg atacacatca tgacaataga tgagatttca aaggttggca
    21721 tcccaaagta gagtggagtt gggacatgga caagtggagc taccgcaaca ggagagaagt
    21781 ccaccaagcc catagtagca gcaatggcag ttccaacaac cagaccaatc aaaatagaga
    21841 tagacttgat aaatcctttg gtaaagatgt tgatcaagag gataatcaga acagtaatag
    21901 ctgcaagcaa gagactttga ccagttggct ctggaacgtt atttcccata tttccaatag
    21961 cgacagggat caaggttaaa ccaatcgtgg taataacaga tcctgttacg atagatggga
    22021 agagattggc tacttttgag aagatgcctg aaacaagaac cacgtaaatc ccagatgcga
    22081 taagggcacc aaacatagcg ccattaccat ggctttgccc aatcataatc aagggagcga
    22141 ccgactggaa tgcaactcca agaacgactg ggagtccaat cccaaagtat ttgttgagtt
    22201 ggagttggag gaaggttgcc accccacaca tgaagatatc tgtagaaatc aggtaggtca
    22261 actgctcagc tgaatagcca agggctgtcg caatcatgat gggaaccagg atagatcctg
    22321 agtacatggc tagtaagtgc tgcaagccca gaacggctgc ttgcgagtgt ttttcttgag
    22381 tttgcattag agatctgcct ccttaaatac gacctgacca ttttcaaaac gatccaagcg
    22441 agcaagtgat aggacagggt agcctgcttt ttcaagcaaa tcacgaccat cttggaagga
    22501 tttctcaatc acgataccga tagcttggac tgtggcaccg gcctgttcga tgatttgaat
    22561 caagccttta gcagcttggc cattagcaag gaaatcgtcg ataatcaaaa ccttgtcctc
    22621 tggtgagagg aattttccag cgatagaaac agtgctggtc acctgcttgg taaaggagta
    22681 gacttgagca gttaagatgc cttcgttcat ggtgatgttc ttagcttttt tggcgaaaat
    22741 catgggaacg tttaaggctt cagctgtaaa aacggctggg gcaatacccg acgcttcaat
    22801 ggttacgacc ttggtaatgc cagtagcagc aaatttttcc gcaaaaacct taccaatctc
    22861 tcgcatcaag ctaaagtcaa cttggtgggt taaaaaggaa tctaccttga ggatgttatc
    22921 acccaagata tgcccatcct tgaggatgcg ctcttctaat aatttcataa gacctcctaa
    22981 agtctaaaag ttaatttact tgttgtttaa atatttctat agtgatccct tttgctaata
    23041 ctatatattt gataaaacta ttacgagcga agcgagtctt atcaaatatt tcccgttgta
    23101 gtggtatcat agacaataat cttgttattg tctatgacgg gatttttgag agtaaaatag
    23161 ttcggggaac tattttagcc taagcctaga aatgaaagag ctaggggctc aaaaattagg
    23221 gatgaaattc cctggattcc tgaaattatt cacaggataa tttcacctcc cgtccgcact
    23281 aattaaggga aatattaaaa atattcaaaa aagacctact taatctctaa gtaagtcccc
    23341 taaatagaca tggcaaaaac ggccatatct cactgctgac ttacttattg ttaggtgttc
    23401 cggcaccttg tagaaacgtc gtgccaattc acgacataaa caagtaaaac gatattcaat
    23461 tttaaatagg cttgagccaa tgtttttatt ttacactaaa taactttaga aatcaactat
    23521 tttgttagtg ttttggttta aaaagcgaac aaaaataaga gagggtgaac aaaaactcca
    23581 ttgtaagcta acatttatac taaatgaaaa tcaaagagca aactaggaag ctatccacaa
    23641 cctcaaaaca ctgttttgag gttgtggata gaattgacag agtcagtatc atatacctac
    23701 ggtaaggcga cgttgacgtg gcttgaagag attttcgaag agtattagaa gatttttcca
    23761 tcataaaagg catactatca agtttttaga cacctgacaa tatgcctttt tctaacttta
    23821 aagacttttc ccaattttta ttattctact cgctaaatct taaaaaatag ccatctggat
    23881 ccaaaactgc aaatttatga ggatagatat agggatcact gacacgaaac tttcttttgg
    23941 tcaagagacg ataaatagga tagtttgcct ccatcacttt ttgatagagt tttggaacat
    24001 cctttatgcc aaaggagaga ttgactccac gaccaaaggg gtaggtcagt tcagctagtt
    24061 gatcctttgt tccctcctct aacattagtt gacactcttc aagagaaaga gaaagttttc
    24121 ttctggacgt tagtattcaa tcctaaaacc cagtaaacca cagtagaagg accgggactg
    24181 ttcgatattc gatacaagaa actcgggaat gaccgcattg tagtccatat agaaaatcct
    24241 tacaagtcaa tttccaagac aatcggtgta tggtcttggc gagcacctga gtcaatcata
    24301 tcagatttag tgaccttgtc agcgatacgg ttacttgtga gccagtagtc gattctccag
    24361 cctgtattgt tgattttaga agttttgctg cgttgtgccc accaagtgta gcgttcagga
    24421 acatcgccat gaacatggcg gaaggtgtct gtaaatccag ttgccaaaag gttggtaaat
    24481 ccagcacgtt cctcgtcagt aaatccaggt gaacggcggt tgctagcagg atttgcaagg
    24541 tcgatttcat tgtgggctac gttgtagtca ccggtcgcaa ggactggttt ttctttgtct
    24601 agttcagcca aatactcagc atatttaaca tcccagactt ggcgttcttc caagcgtttg
    24661 agaccgtcac cagcgtttgg agtgtaaact tgggttacga aaaatgcatc aaattctaga
    24721 gtgatgatac ggccttccaa gtccatggta gaaggggcac cgatttctgg aaaactgatc
    24781 gtaggcgtaa gttctttctt ataaaggaac atggttccag catagccttt acgggcaggc
    24841 tcttgggaag agcgccatgt gttttcgtag cctgggaaga gttcttctaa aatttccacg
    24901 tgtttctttg taggtccttt ggcagaaagc ttggtttctt ggatagcaat gatatcagca
    24961 ttttcagcga ccaaggtttg taggacttct tgggacaatt tggcacgagc tgagtcacta
    25021 gttagggcag cgtttaggga atcaatattc catgagataa gtttcataaa gttacctttt
    25081 tcattcagat tatagatttt attataccaa aaaaagatct atttccccaa cgtatggttt
    25141 gaaaaattac tctctttcgt ttataattaa gaatgatttt atgaaaggga gtgaaaatat
    25201 atgaaattct actcttatga ctatgtactc agccaaatcg gtcagcaaaa tggtatcatg
    25261 gttggctttg ggattgttct attagctgtg acagtttttt ttgctttcaa ggcataccat
    25321 gataaaaagg gaaccgaatt tcgtgagttg gtcatgattt cagctctggc cttatttagc
    25381 tctgcttttg gtcagcatca cgacttatca aaacaatcaa gtttctaaca ataaatttca
    25441 aacttcactt catttcatcg aggttgtttc caaagatttg ggagtagaca agtcagaagt
    25501 ctatgttaat acttccacaa acacagatgg cgcacttatc aaggtgggag atcgctatta
    25561 tcgtgcccta aacggaagtg agccagacaa gtacctgtta gagaaagtcg aattgtataa
    25621 gacagacgca attgaactgg tggatgtgaa caaatgacac ttaattatat cgaaatttta
    25681 atcaaactgg tcttgactct caaatggctc aacaacaatg ttcactttgt gaaacgtttg
    25741 attgatggta agccaactct ccttatcaaa aatgggaata ttgacccaga agcctgtcgt
    25801 tcagttggtt tgtctgcatc ggatgtagcc ctcaaacttc gtagccaagg gattttccag
    25861 atgaagcaag tcaaacgagc tgtgcaagag caaaatgggc aacttatcgt tgtgcaaatg
    25921 ggagatgaaa atcctaagta tccagttgtg actgacggtg tgattcaagt agatgtcttg
    25981 gaatcgattg gtcgtagcga agagtggttg cttgataacc tcagtaaaca agggcatgac
    26041 aatgtagcca atatctttat tgctgaatat gacaagggtg ctgttacagt cgtaacttat
    26101 gaaaaagaaa aacctggggt cttgtactct tcgaaaatct cttcaaaccg cgtcaacgtc
    26161 gccttgccgt atgtaggtta ctgacttcgt cagttctatc tacaacctca aagcagtgct
    26221 ttgagcagcc tgcggctagt ttcctagttt gctctttgat tttcattgag tattggcctc
    26281 aggtttccat ttgcaatcag aaagggattt tatgtccatt attcaaaaac tttggtggtt
    26341 tttcaagtta gaaaaacgcc gttatctagt cggaattgtg gccctggtct tggtttccgt
    26401 cctcaatctc attcctccta tggttatggg gcgggttatt gatgccatca catcggggca
    26461 attaacccag caggacctcc ttcttagcct attttacttg ctacttgcag cctttggtat
    26521 gtactatttg cgctatgtgt ggcgtatgta tatccttggg acctcttatt gcttgggaca
    26581 gatcatgcgg tctcgcttgt ttaagcattt cacaaaaatg tcgtcagcct tttatcaaac
    26641 ctatcggacg ggtgatctga tggcacacgc aaccaatgat atcaatgcct tgactcgttt
    26701 agcaggtggc ggtgtcatgt ctgcggtgga tgcctctatc acggctctgg tgactttgtt
    26761 gaccatgctc tttagcatct catggcagat gactcttgtt gccattctcc ccctaccttt
    26821 catggcctat gcgactagtc gcctagggag aaagactcat aaggcctttg gcgaatccca
    26881 agctgctttt tctgaactca ataacaaggt acaggagtcc gtatcaggta tcaaagtgac
    26941 caagtctttc ggttatcagg cagacgagtt gaagtctttt caggcagtca atgaattaac
    27001 cttccaaaag aacctgcaaa ccatgaaata tgatagtctc tttgacccta tggttctctt
    27061 gtttgttggt tcgtcctatg ttttaacgct tttggttggc tccttggtgg ttcaggaagg
    27121 gcagattaca gttgggaatc tagtcacctt tatcagctat ttggatatgc tggtctggcc
    27181 tcttatggcc atcggcttcc tctttaatac tactcagcga gggaaggttt cttaccagcg
    27241 gattgaaaat cttttgtctc aggaatctcc tgtacaagac ccggagtttc ctctggatgg
    27301 tattgaaaat gggcgtttgg agtatgccat tgacagcttt gcttttgaaa atgaggaaac
    27361 actgacggat attcacttta gtttggcaaa agggcaaaca ctgggcttgg ttgggcagac
    27421 aggctctggg aaaacgtcct taatcaagct cctcttgcgt gaatacgatg tggataaggg
    27481 cgccatttat ctaaacggtc acgatattcg ggactatcgt ctgacagacc ttcgcagtct
    27541 catgggctat gttcctcagg accagtttct ttttgcgact tcaatcctag acaatatccg
    27601 ctttggcaat cctaacttgc ccctttcagc ggtcgaggaa gctactaagc tagcccgagt
    27661 ttaccaagat attgtagaca tgcctcaagg atttgatacg ctgattggtg aaaaaggagt
    27721 cagtctttct ggtggtcaaa agcaacggtt ggctatgagt cgggctatga ttttagaccc
    27781 tgatatcttg attttggatg attccttatc cgccgtagat gccaagacag agtatgcgat
    27841 tatcgacaac ctcaaggaga cgcgaaagga caagacaacc attatcactg cccatcgcct
    27901 gagtgcagtt gtccatgcag atttgatttt agtcctgcaa aatggtcaaa ttatcgaacg
    27961 aggcaggcac gaagacttgc tagctttgga tggctggtat gcccaaacct accagtctca
    28021 gcagctggaa atgaagggag aagaagatgc agaataaaca agaacaatgg actgtattga
    28081 agcgcttgat gtcctatctc aagccttatg gactccttac ctttttggca ctcagttttc
    28141 tcctagcgac gacggtcatt aaaagtgtca ttccccttgt ggcttcccac tttatcgacc
    28201 agtatctcag caatcttaac caactagccg tgaccgtttt gctggtctac tatggtctct
    28261 acatcctaca aactgtagtt cagtatgtcg gcaatcttct ctttgcgcgc gtgtcttaca
    28321 gtattgttag ggatattcgt cgggatgcct ttgccaatat ggagaaactg ggcatgtctt
    28381 actttgacaa gacgccagca ggttctatcg tttctcgttt gaccaacgat accgagacga
    28441 ttagtgatat gttttctggg attttatcca gctttatctc agcagttttt atctttctga
    28501 caacccttta taccatgttg gtgctggatt ttcgtttgac ggctttagtc ttgctctttc
    28561 tccccttgat tttccttttg gtcaatctct atcgaaaaaa gtcagtgaaa atcatcgaga
    28621 aaaccagaag tctcttgtca gatatcaata gtaagctggc agagaatatc gagggaatca
    28681 ggattattca ggcttttaat caagagaagc gcctgcaggc agaatttgat gaaatcaacc
    28741 aagagcactt ggcctatgcc aaccgttctg tagccttgga tgccctcttt ttgagacctg
    28801 ccatgagttt gttgaaactt ctaggttatg ctgtcttgat gacctatttt ggctatcgtg
    28861 gtctttctat cgggataacg gccgggacca tgtatgcttt tatccagtac atcaaccgcc
    28921 tctttgaccc tttgattgag gtgacgcaaa acttttcaac actgcaaacg tctatggttt
    28981 ctgcaggccg tgtctttgcc ttaatcgatg agaggaccta tgagcccctt caagaaaatg
    29041 ggcaagccaa agtcaaagaa ggcaatatcc gttttgaaca tgtgtgtttc tcgtatgacg
    29101 gtaaacatcc gattctggat gacatttctt tctctgttaa taagggtgaa accattgcct
    29161 ttgtaggtca tacaggttca gggaaatcgt ctattatcaa tgtcctcatg cgcttttatg
    29221 aattccagtc agggagagtt ctcttggatg atgtggatat cagggatttc agtcaagaag
    29281 agctgagaaa aaacatcggt ttggttttac aggaaccctt cctctatcat ggaactatta
    29341 agtccaatat cgccatgtac caagaaatca gtgatgagca ggttcaggct gcggcagcct
    29401 ttgtggatgc agattccttt attcaagaac ttcctcaggg gtacgactcc cctgtttccg
    29461 agcgtggttc gagcttctct actgggcagc gccagcttct tgcctttgct agaacagtcg
    29521 ccagccagcc taaaatcctg attttggatg aagcgacagc caatattgac tctgaaacag
    29581 aaagtttggt tcaagcttct ctggcgaaga tgagacaggg ccgaacaact attgctatcg
    29641 ctcaccgcct ttctactatt caagacgcca actgcatcta tgtcttggat aagggacgca
    29701 ttatcgagag tggaacccat gaggaactct tgactctggg aggaacctat cacaagatgt
    29761 atagtttgca ggcaggggcc atggcctata ctctttgaaa atctctttaa accatgtcag
    29821 ctttatctgc aatctcaaag ctgtactttg attttcattg agtactagaa ggaaatcctt
    29881 caaattacag atttctttca ccgccttttc cattttgtgg tataatgaaa aatgttgaca
    29941 aatagtataa taaaaacaaa ggagaacagc atgctgaaat gggaagactt gcctgtggaa
    30001 atgaaatcaa gcgaggttga gtcttactac cagcttgtct ctaaaaggaa gggttcgctg
    30061 attttcaagc gttgcttgga ctgggttttg gccttggtct tactggttct aacttctccc
    30121 atctttctca tcttgagcat ttggatcaag ttggatagca aggggccagt gatttacaag
    30181 caagagcgtg tgacccagta caaccgtcgg ttcaagattt ggaagttccg taccatggtg
    30241 acggatgcgg ataaaaaagg aagtctggtg acttctgcta acgatagccg tattaccaag
    30301 gttggaaatt tcatccgacg tgtccgtttg gacgaactgc ctcagttggt caatgtcctt
    30361 aaaggtgaga tgtcctttgt cggtacacga cctgaagtgc cacgttatac agagcagtat
    30421 agccctgaaa tgatggcaac cttgctcttg caagcaggaa ttacctctcc agccagcatc
    30481 aactacaagg atgaggacac catcatcagt caaatgacgg agaaaggtct gtcagttgat
    30541 caggcctatg tggagcatgt tcttcctgaa aagatgcgct ataacctcgc ctatctccga
    30601 gagtttagtt tctttgggga catcaaaatc atgtttcaaa ccgtgtttga ggtactaaaa
    30661 taaagtagtc ataagaaaat gagtacagat aaaaggagca aatcaatgcc aaattacaat
    30721 attccatttt caccgcctga tatcacagaa gcagaaattg ctgaagtagc ggataccctg
    30781 cgttctggtt ggatcacaac aggtcctaaa acaaaagaac tggagcgccg cttgtctctt
    30841 tacacacaga cacctaagac tgtttgtctc aactctgcga cagccgctct ggagttgatt
    30901 ttacgcgttt tggaagtggg acctggtgat gaagtcatcg ttccagccat gacctatacg
    30961 gcttcatgta gtgtcattac gcacgtggga gcaacccctg tcatggtgga tatccaagca
    31021 gatacgtttg agatggacta tgacctgctt gagcaagcta tcactgagaa aactaaggtg
    31081 attatcccag tagagctcgc agggattgtt tgcgattatg accgtttgtt ccaagtcgtg
    31141 gagaaaaaac gtgacttctt taccgcttca agcaagtggc aaaaggcctt taaccgtatt
    31201 gtcattgtct ctgatagtgc ccacgctttg ggatctactt ataaaggaca accttctggt
    31261 tctatcgctg attttacttc cttctcattc catgctgtta agaactttac aacggcagaa
    31321 ggtggaagtg cgacttggaa agccaatcca gtgattgatg acgaagagat gtacaaggaa
    31381 ttccaaatcc tttcccttca cgggcaaact aaggatgctc ttgccaagat gcaactgggg
    31441 tcatgggaat acgatatcgt tacaccagcc tataagtgca acatgaccga tatcatggct
    31501 tcacttggtt tggtacaatt ggaccgctat ccaagtttgt tgcaacgccg taaggacatt
    31561 gtggaccgct atgatagtgg ttttgcaggt tctcgcatcc atcctttggc acacaagact
    31621 gaaactgtcg aatcttcacg ccacctctac atcacccgtg tagaaggagc aagcctagaa
    31681 gaacgcaacc tcatcatcca agaattggct aaagcaggaa ttgcaagtaa tgttcactac
    31741 aaaccgcttc ctctcttgac agcctataag aatcttggat ttgatatgac gaactatcct
    31801 aaggcctatg ccttctttga gaatgaaatt accctccctc ttcatactaa attaagcgat
    31861 gaagaagtag actatatcat tgagactttc aaaacagttt ctgaaaaagt gctaacttta
    31921 tcaaaaaaat gacaaactac agtcaagcga aagtgatcct gtccctaaaa agtctaattg
    31981 agtgtaaaaa ctgttgtttt caattgataa tagtttacac ctgtagttga ggcctctttc
    32041 tcctcagaga gagaattttt ataggatttt cctttcttgt gggagtcccg tggtttgaaa
    32101 taagatgtga gcaatttggt atagcattta gaatccttac tagacatcat ttagaaaatc
    32161 tagtgtcttg ttctagtttt caattcaccc tattttttga aagacgtgag tttccatgag
    32221 tgagattgtg gaaactagcg tctttttttt gttttcagaa tattgttcaa agttttgtgc
    32281 ctgtctttca tgttctagtc attcttttgc atgatagaat ttatagcatg ttgatattat
    32341 aataacgcaa atatcccata tgtttagtga tgcttgctat acatcattag atctcctgcg
    32401 agacaatcta taaaacactt gtctacgatt acctatatgc cctattccag tattttagaa
    32461 gcactgcatc tatttttatc gaggttaaat ctagctttta tagaaggtct atttaagaaa
    32521 tatattgtag tgttttagtt tcaatccgcc atatgagcga tattcaggta aatatccctg
    32581 gcgaatgctt gtatgacaag gtatttgttc tttcatttat aatttacaac atatcaacaa
    32641 atttaaatat agtaaatggg atattttata ttcaagctaa gaaagatagc atcacttttg
    32701 aatggaaggc taaagagcaa actaggaagt tggccataga tagctcaaaa ccctgctttg
    32761 aggttgtaga tatagtaaaa tgaaacaaga ataggacaaa ttgatcggga cagttaaatc
    32821 gatttctaac aatgttttag aagtagaggt gtactattat agtttcagtc tactatagaa
    32881 ctgaccaagt cagtaaccta gacttagggc aaggcggcac tgacctagtt tgaagagatt
    32941 tccgaagagt ataaatttta atattttctt gtgttattcc ttgacaattc aatttggaaa
    33001 atatatgata aagataatga cagcggtgtc attctatcta ttttaagaaa agtaataatc
    33061 aattgttaaa aatagtaaaa aaattggagg ttctgatgaa atattttgtt cctaatgagg
    33121 tattcagtat tcgtaaatta aaggtgggga cttgctcggt actattggca atttcaattt
    33181 tgggaagcca aggtatttta tcggatgaag ttgttactag ttcttcaccg atggctacaa
    33241 aagagtcttc taatgcaatt actaatgatt tagataattc accaactgtt aatcagaatc
    33301 gttctgctga aatgattgcc tctaattcaa ccactaatgg tttagataat tcgttaagtg
    33361 ttaatagtat cagctctaat ggtactattc gttccaattc acaattagac aacagaacag
    33421 ttgaatctac agtaacatct actaatgaaa ataagagtta taaggaagat gttataagtg
    33481 acagaattat caaaaaagaa tttgaagata ctgctttaag tgtaaaagat tatggtgcgg
    33541 taggtgatgg gattcatgat gatcgacaag caattcaaga tgcaatagat gctgcagctc
    33601 aagggctagg tggaggaaat gtatattttc ctgaaggaac ttatttagta aaagaaattg
    33661 tttttttaaa aagtcataca cacttagaat tgaatgagaa agctacaatt ctaaatggta
    33721 taaatattaa gaatcaccct tccattgttt ttatgacagg tttatttacg gatgatggtg
    33781 cgcaagtaga atggggccca acagaagata ttagttattc tggtggtacg attgatatga
    33841 acggtgcttt gaatgaagaa ggaactaaag caaaaaatct accacttata aattcttcag
    33901 gtgcatttgc tattgggaat tcaaataacg taactataaa aaatgtaaca ttcaaggata
    33961 gttatcaagg gcatgctatt caaattgcag gttcgaaaaa tgtattagtt gataattctc
    34021 gttttcttgg gcaagcctta cccaaaacga tgaaggatgg gcaaatcata agtaaggaga
    34081 gcattcagat tgaaccatta actagaaaag gttttcctta tgccttgaat gatgatggga
    34141 aaaaatctga aaatgtgact attcaaaatt cctattttgg caaaagtgat aaatctgggg
    34201 aattagtaac agcaattggc acacactatc aaacattgtc gacacagaac ccctctaata
    34261 ttaaaatttt aaataatcat tttgataaca tgatgtatgc aggtgtacgt tttacaggat
    34321 tcactgatgt attaatcaaa ggaaatcgct ttgataagaa agttaaagga gagagtgtac
    34381 attatcgaga aagcggagca gctttagtaa atgcttatag ctataaaaac actaaagacc
    34441 tattagattt aaataaacag gtggttatcg ccgaaaatat atttaatatt gccgatccta
    34501 aaacaaaagc gatacgagtt gcaaaagata gtgcagaata tttaggaaaa gtatcagata
    34561 ttactgtaac aaaaaatgta attaataata attctaagga aacagaacaa ccaaatattg
    34621 aattattacg agttagtgat aatttagtag tgtcagagaa tagtatattc ggtggtaaag
    34681 aaggaattgt tattgaggat tcaaagggta aaataaccgt tttaaataac caattttata
    34741 atttatccgg taagtatata tcattcatca aatctaatgc aaacgggaaa gaacctgtta
    34801 tacgtgatag cgatggtaat ttcaatattg taacggagaa tgggctttac aaaattgtaa
    34861 caaataattt aagtgataaa aacgaaaaag aaaaaaacaa agaggaaaaa caatctaatt
    34921 caaataatgt aattgatagt aaccagaaga acggagagtt taactcaagt aaagataata
    34981 gacaaatgaa tgacaagatc gacaataaac aagataataa gacagaagaa gtaaactata
    35041 aaatagttgg agatggcaga gaaactgaaa atcatattaa taaatctaaa gaaatagtag
    35101 atgtaaaaca aaaattacca aagactggtt cgaacaagat tatggaacta ttcttaacag
    35161 tgacaggaat tggtttactt ttgacactaa aagggttgaa gtattatggt aaagataaat
    35221 aaaatttgtt cgatacaagg aagttccgta gaaaatgagg atattgtagg ttcacaaaat
    35281 cagtattttt ggattattgg tggagctaca gatttatata attctaaaga ggagataggt
    35341 tattcagtct cagaagttgt acatatttta tcagaaagtt tgtcggttaa ttgtaaagaa
    35401 tcaaaaactc ttaaacaaat atttgaaact gctttactcg aggttaaaga tgaaatcggt
    35461 ttaaactcat atgaattaac agagtataat aaaatgaaat aataatagga caaatcaatc
    35521 aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtttc
    35581 aatatactat attatcgctt accaacattt gcttttatat ttgctaaact ttcagaaaaa
    35641 aagttggagt atatgatgct aggtgactgc gtcatgttag taaacgaaat ggaaataacg
    35701 gatcatagag tagataactt atttgaaaaa gggaaaaatg agataaaaga tccaattggt
    35761 acaaatagtg ttttaaataa aaaaattatt ttacaaaaaa tcagaaaatt atctaatcaa
    35821 ccgagtgggt actggatagg ttcgttggat gagagatttt tagaccatgc tattattaat
    35881 caaatagatg ttactagtga acaaattgtt ttaatgagcg atggctttta tgaattctat
    35941 caaaataatc aaaataaaac ttttgaagag ttaattaaga tgagatttaa ttcttctgct
    36001 attgatccaa tctatggaaa gaaagatgat gcaagtattg tagttattga tgtttgatat
    36061 attgtttaaa atattaattg ttactattga actttgttaa gaagaggtac ctaaatgtta
    36121 aggttatatt tagaaaatga aattatagaa ttatcaaata atttagagac catttgggta
    36181 tctgtgcttg ataaatatga gaagttattt gaatatttat caaatgaaga acgaatagaa
    36241 ttgattaaag aagatttaat cataaatgag tcagttctaa atgttgataa aaaaggatat
    36301 gagttaattt gtttacaaca tcccgtttca tatgacttaa gaagaattat tagtgtaata
    36361 aaaatatcaa ctgacattga acgtattggg gatagaattg ttgaaatttt aaaaaatcta
    36421 cagattattc aaaataatga aattttgaag aaaattattt cagaaataaa aatacttcat
    36481 gaagttatcg gactgcatat gaatagggct atatcttgtt atagagagga acaatctgga
    36541 tgtttagata tggttgttat acaaaaacag aatgagatag aagagcttag tacaaatatt
    36601 gaaaagaaaa taatgaatta catatttgaa gatgatggga atgtttctga agtaattggt
    36661 gctttagata ttattcatca ccttgataaa attgctcata ctacgcaatc aatttataaa
    36721 tggataatgt atagaaaata tgggaacata aattagaaag aaaaatatat gaaaacaatt
    36781 attgataatt ttgttgatag agtaattgaa tacggaatgt ataatgaaat tgacaaaata
    36841 tatgtgaaaa atagagtact agcgttaata ggtgaggagg gaactgatcg catttccgat
    36901 gaaaatgacc tgaaacaaat aaaagattac ttagtggaaa tcgccttaaa aaacggtaaa
    36961 attaaagatt taatcgagga aaaggaatgt ttaggagcag agttaatgaa tttcatagtt
    37021 cctttaccta gtcgattaaa tgatattttt tggagttcat atgatatatc acctcaagaa
    37081 gcagttgaag agttttataa gttaagtaaa gatagtgatt atattaaaac ttctgctatt
    37141 gctaagaata ttgagtttcg agcatcaact aaatacggag agctagagat tacgataaat
    37201 ttgtcaaaac ccgaaaaaga tccaaaaaca attgctgctg aaaaattagt aaaagccact
    37261 aattatccca aatgtttact atgtatggaa aatgaaggat atcaggggcg aataaattat
    37321 ccagcacgtt cgaatcatag aattattcga ttgaaacttg gtgacgaagt atggggattc
    37381 caatattctc cctacttata ctttaatgag catgcgatat ttttaaatag tcaacatgta
    37441 ccaatggcta ttacctctaa aacatttgaa caattattgg agattgttga tattttgcca
    37501 ggatattttg ctggttcaaa ttctgatctt cctatctcag gaggctccat tcttagtcat
    37561 aaccactacc agggagggaa acatattttc ccaatggaaa aagcaaaatt tgagagtgaa
    37621 tttcgtttta aagactttga ggatgttaat gctggtattg taaaatggcc aatgtcagta
    37681 attaggttac aaagtgaaaa taaaaatcgc ttgttagatt tagctactaa aattctaaat
    37741 aagtggagag aatattcaga tttagaagta gacatcattg ctatgactga agatgtccca
    37801 caccatacgg tgactcctat tgctcgtaaa gtggacggga gatatgaatt agatattgtt
    37861 ctaagagata atcatactac tgaacagtac ccagatggtg ttttccatcc tcatcaagat
    37921 gtacaacata ttaaaaagga aaatatcggg ttaatcgaag tgatgggact tgcgatatta
    37981 ccacctcgtt tgaaaccaga gttagaagaa gttggaaaat atcttttagg agaggataac
    38041 gcaattgcgg attatcattt agaatgggcc gaccaattga aagaaaaata tcctcgcatt
    38101 aataaagaag aggttaatag cgtagttcaa cacgaagcag gacaagtgtt tgcgagagtt
    38161 ctggaagatg caggagttta taagaataca ccgtctgggc atgaagcgtt tatgagattt
    38221 gttaagtcgg ttggaattaa ttaggaggaa acaaaaatgg caatattggt aacaggcgga
    38281 gctggttata ttggtagcca taccgtagta gaattactaa atttaggaaa ggaagtcatc
    38341 attgtcgaca acctttcgaa ctctagcatc ttggtgctag accgtattga agcaattaca
    38401 ggaatacgtc ccgtgtttta cgaattagat gtttgtgata aaccagcatt gagaaaggta
    38461 tttgaacaag aatcgattga tgctgcaatt cattttgcag gttataaagc tgtcggagaa
    38521 tccgtgcaaa agcctgtgat gtactacaaa aataatatta tgagtacatt ggcacttgtt
    38581 gaagtgatgt cagaatttaa tgttaaaaag attgtctttt cttcaagtgc gactgtatat
    38641 ggaattaaca atcagtcacc tctaattgag acgatgcaaa caagtgcgac aaatccttat
    38701 gggtatacga aagtgatgct tgagcaaatt ttaaaagatg ttcatgtggc agattcagaa
    38761 tggagtattg cgttgcttcg ttatttcaat ccaattggtg ctcatgagtc tggtttgatt
    38821 ggggaagatc cctcaggact tcctaacaac ttgatgcctt atattgcaca agtagcggta
    38881 ggtaagctat cagagctaag tgtgttcgga aatgattatg atacgcttga tggtactggt
    38941 gtgcgtgatt atatccatgt agtagattta gcaataggac atataaaagc tttagaaaaa
    39001 gtatctgaaa aaacagatgt ttatatctat aacctaggct cgggagaagg cactagtgta
    39061 ttacaacttg taaatacatt tgaaagtgtt aataagatcc ctattcctta taaaatagtt
    39121 ccaagacgtt caggagacgt tgcaacttgt tatgcaaatg cagacaaagc atataaggaa
    39181 ttaaattgga ggacaacaaa atcgattgaa gacatgtgta gagatacatg gaattagcaa
    39241 tcaaaaaatc ccaatggcta taattgatga tactgtctat atgagtgctg tattttggaa
    39301 aaattactta accttttaat gaaatgcata tttaatgaaa gataaattaa aaaaaatgtt
    39361 tgacagcgct tgacaaagga aacggtttca ttgtataatg taaacatagt gacaccgatg
    39421 tcaaatataa aaaagaaggt ctttatatga aaaacttaac aaaaattaag ttcaaagaaa
    39481 atggggaatt taatcatttc cctgggaata cagttgtagc aaatctttat actaaacaag
    39541 atttgatgga agttgttgat attattcaat cacgttatag agaattacca tttatcgata
    39601 agtttacttt aactccaagg aattctattc acatgactgt aattgaattg ttgtgccatg
    39661 aaaatcgtga aacggagttt tggagcagta atcttcctct agatacacct ttacaggaaa
    39721 tacatgatta ctttgctaaa caacttgaaa tttttccatt gttggatgaa gaaattcata
    39781 tgcgtataac tgaaatggga aaacaaaaca tactagttga acctgcagat gaagcttctg
    39841 caaagagatt agaagaaatt cgtacttatg tctcagaaaa agcaggtgtt tgtttcccta
    39901 atcatgatag atatcaattc catatttcaa ttgggtatct tcggattcct ctaaccgaag
    39961 aggaggaaga agagtttact aaagtcagag cagaattaac tgaaatttta ttagagaaga
    40021 ttccaactat tactgtaaac cgtattgact atactgtatt tgaagatatg agacaatttg
    40081 ttccatatca cgaaaaattt aaataaaata ataaaaaagg agaacttatc atgaaaaaaa
    40141 tgaaagtttg gtctactgta cttgcaacgg gagttgctct tactacactt gctgcttgct
    40201 ctggaggttc aaattctacg actgcttctt catctgaaga aaaagctgat aaaagtcaag
    40261 aattagttat ctattcgaac tcagtctcaa atggtcgtgg tgattggtta actgctaaag
    40321 caaaagaagc tggttttaat ataaaaatgg ttgatatcgc tggcgctcaa ttagcagacc
    40381 gtgttattgc tgagaagaat aatgcagttg cagatatggt atttggaatt ggtgctgttg
    40441 actcaaataa aattagagat caaaaattac tagtacagta caagcctaaa tggttagata
    40501 aaattgatca atctttatca gataaagata attattataa tcctgtgatt gttcaaccat
    40561 tagttttaat tggggcgcct gatgtaaaag aaatgcctaa agactggaca gagttagcta
    40621 gtaagtataa aggtaaatat tcaatttatg gtcttcaagg tggtacagga cgggcaattc
    40681 tagcaagtat cttagttcgt taccttgatg aaaaaggaga tttaggcgta tcagaaaaag
    40741 gatggggagt tgcaaaagaa tactttgcaa atgcctatac tcttcaaaaa ggtgaaagct
    40801 cagttgttaa agcgttagat aaagaaagcc caattcaata cggtatgatc tggggttctg
    40861 gagcgttaat cggacaaaaa gaacaaaatg ttgaattcaa agttatgact cctgagattg
    40921 gtgtaccatt tgtaactgaa caaactatgg ttttaagcac tagtaaaaaa caagcgttag
    40981 ctaaagaatt tattgattgg tttggtcaat cagaaattca agtagaatat agtaaaaaat
    41041 ttggatctat tcctgcaaat aaagatgccc taaaagagtt acctgaagat acgaaaaaat
    41101 ttgttgatca agtgaaacca caaaatattg actgggaagc tgttggaaag catttggatg
    41161 aatgggtaga aaaagctgaa ttagaatacg taaaataaga aataagagtt attttttatg
    41221 tccaagtctc tctattattt ctaaatttaa ggaagaaata aagagaggct tttggtttag
    41281 taatagaggg agttagtgag atgattaaat ttgataatat tcaaattaaa tatggtgatt
    41341 ttgttgcaat tgataatctg aatttagata tacatgaagg ggaattcttt acatttcttg
    41401 ggccttcagg atgtggtaaa tcaactactt tgagagcatt ggtaggtttt ttagatccat
    41461 catcaggaag tattgaagtt aatggaacag atgtcactca tttggaacct gaaaagcgtg
    41521 gaattggtat tgtatttcaa tcttatgcgc tatttccaac tatgactgtt tttgataata
    41581 ttgcatttgg tttaaaagtt aagaaggtag ctccagatgt tattaaagct aaagtatcag
    41641 cagtggcagc aaaaattaag atctctgatc aacagttaca gcgtaatgta tcagaattat
    41701 ctgggggtca acaacaaagg gtagcattgg ctcgtgctct ggttcttgaa cctaaaattc
    41761 tttgtctaga tgaaccattg tcaaaccttg acgcaaaatt acgtgtagat ttgagaaaag
    41821 agttgaaaag acttcaaaaa gagttaggta ttactacttt atatgttact catgatcaag
    41881 aggaagcctt gactttatct gatagaattg cagtctttaa caatggatac atcgaacagg
    41941 tcggtacacc agtagagatt tatcataatt ctcaaactga atttgtatgt gattttattg
    42001 gagatattaa tgttttgacc gatgaaacag tccacgaagt attactgaaa aatacaagcg
    42061 ttttcttaga ggataaaaaa ggttacattc gattagagaa agttcgattc aatcgtgaaa
    42121 ctgaacaaga ttttattcta aaagggacaa ttattgatgt tgagttttct ggagttacaa
    42181 ttcactatac aataaaagtt tctgaaagtc agattcttaa tgtaacaagt attgatagtc
    42241 aggctgctat tagatctgtc ggagaaagtg tggaattatt tatcacacca tcagacgttc
    42301 tgcaatttta aggaggtgca gtatgcgtca taaattaaat ttaaaagatt ggcttattcg
    42361 tttagggtta atctggttct tagtaacatt tattatttat ccaaactttg atctagtagt
    42421 gaatgtattt gtaaaaggag gagaattttc ccttgatgct gtacatcgtg ttctaaaatc
    42481 tcagagggca cttcagagta ttatgaacag ttttaagtta gcattttcac tcattattac
    42541 agttaatgtc gtaggtattc tttgtgttct atttacagag tactttgata ttaaaggtgc
    42601 taaaatttta aaattaggtt atatgacctc tttaatttat ggaggagtgg ttttagcgac
    42661 tggatataaa tttgtctatg gtccttatgg attgattaca aaatttttac aaaatgttat
    42721 cccttcttta gactctaact ggtttattgg gtatggtgca gtcttattca ttatgacatt
    42781 ttcaggaact gctaatcata cattgttttt aacaaataca attcgaagcg ttgactatca
    42841 cactattgag gctgctcgaa atatgggagc aaaaccattt actgttttcc gaaaagtagt
    42901 gttaccaacc ttaattccaa ctctatttgc acttactatt atggtttttc ttagtggttt
    42961 atctgcagta gcagcaccca tgattgttgg tggtaaagaa tttcaaacta taaatccaat
    43021 gattattaca tttgcaggga tggggaattc tcgtgattta gctgccttac ttgcaattat
    43081 tttaggtatt gcaactacaa ttttgcttac tatcatgaat aagatagaaa aaggtggaaa
    43141 ttatatttct atctctaaga ctaaagcgcc tcttaaaaaa caaaaaattg cgtctaagcc
    43201 ttggaatatc attgctcaca ttgtagcata tggattgttc acagttttca tgcttccact
    43261 aatttttata gtattatact catttacaga tccagttgca attcaaacag gtaacttaac
    43321 attatcaaac tttactttag aaaattatcg cttattcttt agtaatagtg cggcattctc
    43381 tccattcttg gtcagcttta tttattctat tattgctgcg acaacagcaa caattctcgc
    43441 agttgtattt gctcgtgttg tcagaaaaca taaatctcgt tttgatttct tatttgaata
    43501 tggtgctcta cttccttggt tactaccaag tacactttta gcagtaagtt tattatttac
    43561 ttttaatcag ccacaatttc ttgtcttgaa tcagattttg gtaggtagtt tggtaattct
    43621 acttattgca tatatagttg taaaaatccc attttcttat agaatggtac gtgctatttt
    43681 atttagtgtt gatgatgaga tggaagatgc agcaagaagt atgggtgctt caccttttta
    43741 tactatgatg aaggttatca ttccatttat tttaccggtt gttctctctg ttattgcttt
    43801 aaactttaac tctttattaa ctgacttcga cttatctgta ttcctttacc atcccctagc
    43861 tcaaccatta ggtattacga ttcgatctgc aggtgatgaa acagcaacat ctaatgcaca
    43921 agctctggta tttgtttata caattgttct gatgatcatt tctggaacgg tattatactt
    43981 cacacaaaga ccggggcgta aagtaaggaa ataatcatga cagccactag tcttgggtta
    44041 tcaaatattg aaatagttgt caggattgtt ttatcagtag tcattggtag tataattggt
    44101 ttagagagag ggagcaaatc ccagcctgca ggcatccgaa cttatagtat tgtttgtcta
    44161 gctgcatgtt tgattatgat gacgaatgaa tacgtatctt ataaatttgg gacaggagat
    44221 cctacacgat taggagctca agttatatca ggtgtgggtt ttctaggcgc tggaacgatt
    44281 cttattacag ataaaaagaa aattacaggt ctgacaactg cagcaggcat ttgggcttcg
    44341 gcaggaattg gattagctat tggagtaggt ttttatgagg gagctctttt agtagccatt
    44401 tctgtttggg gtgtgatatc catgttccaa ccactaaaaa aatatctgca aaatcgttct
    44461 aaaatgattg aattgtatat agtggttaaa tctacagaag cctataatcg tgtattggtt
    44521 tattgtgcag aaaatggtat cagaatgacc gattcaagaa cagcgtttgg agatgttaat
    44581 tctgacagaa ttgaatactt tgatgttccg gacaaaaaaa tagcgtcatt tattactctg
    44641 gaattatcag gtagatttga acatcttcga ctgatggaag aaattgctaa tattgtaggt
    44701 gttatttatg tcgaagaaat tagctgaaat ttagtaaaca aagggacaga tgtttttgtc
    44761 cttttgtttt ttagatgttt taatcatcta atttttgagt agatttttgc tcagaggttt
    44821 ttatgaaaga ttataaaatc aattttgatt taggtaaaat agaatatttt gataataatt
    44881 gtttgataca agtatacaaa tttatttctt tttatgatat ttgtgaaatg gtgtttgcct
    44941 ttcatttacc tcctgatgag ttgataacaa atgttatctt taaagaaaaa attaactcta
    45001 tgttaaaatg ttacattgac aggctattat atgtttttat aaatcctaca cattttactg
    45061 aaaaagttaa tctacaattt tatggtagtt ttttctcata tgaatttatt tgtcgtgaag
    45121 ttggaaacat tcttaaaaat aaaggtgtga aatgcaattt aaactttttt gaaggggaag
    45181 aatatttgta aaattaattt tgtgaatttg tggatcttaa ataatttact aaattctctt
    45241 gttaaaaaaa attaaatatt gtatagtaat attagaatgt tcgtaatgga ggactgaaat
    45301 gaaaaatata aatgggaaaa aggtaactat ttatgatatt gcacgtttat caggtttttc
    45361 tccaaaaaca gtttcacgag ttattaatgg tggagtaaat gttaaagaag aaacttatca
    45421 agcgattcaa aaagttatag aggaattgtc gtatattcca aatgcgtatg caaaaaattt
    45481 aactaaaaaa gaagctataa atatattgat ttctgtaaaa aaaatcgact cttttccttt
    45541 aatttggttt catacgctgt tagataaagt gttacggacg tgtaaagaat ttggtgttaa
    45601 tgctatagtt gagtactttg gagaggagga tacaattagt aattcaatta tatcaagcac
    45661 aggtagccta gtagatggtg ttattgtttt ttatgaaagt gtagatgata tcagaattca
    45721 gtatttaaag aagaaccata tgccttttct tgttttcggt gaatctcaaa cgtctggagt
    45781 agtctatgta tctaataata attttcaagc tacttatgat atgatgaaag ctgtaactga
    45841 agaaaaattt aaaaatatgt ggttgcttat gggaggagaa tcccatgtta ataaggatcg
    45901 tgaaagaggt gtgcgttctt ttctgaatga taaaaattac tttatggatt taaaagtcat
    45961 ttatggctta tctacaattg attcagttta ttcttatgca atgcagcact tgactaccca
    46021 aaattatccg gacattattt ttgtttcagg agatgaaaag gttcagggct tgattcgtgc
    46081 gtgctatgag aaaggaattc ttattccaga tgatatttct atcattggat ttgataatat
    46141 tcctatttct cagtattata ctccagcttt gtctacaatt gctcccaatt atgttaaatt
    46201 ggctaaagaa atgatagaag gtgttttagc aattattaaa ggggaaagtg tcacatctgt
    46261 tgaagtttct cctaaatttg ttaggagaca gagtttctag tattgtttaa ttcttgaaaa
    46321 tcctagtgga aaagatggag tgttctaaaa taagttctgt ttagcctcca ttcagtttct
    46381 attgagttgg atccaatttc taacaagtgg gacatgttca tgttcgatct aaggaagaca
    46441 tggtcacatt cgaatgatat tttcaaaaaa atcgtaaaaa agtcttgaca aagaaaaaac
    46501 aaagtattaa aataagttca acaaatcaga aaagtaacta tttttgatct tcagggagcc
    46561 tgtggtggtt gtgaataggt ggttggaagt agtgaaagtg ggctgatttt gaaaatgaat
    46621 ttgaaacaat gaaaattcgg tgcgcacacc ttacagtgca gcttgttgtt aaacaaggca
    46681 gagatgtaaa gggatagtcc ctttataatt gaggtggcac cgcgttacca acgccctcac
    46741 acggaagtat attttgtgtg tgggcttttt tctatccgtc gtttggttta tcttttatta
    46801 gggccttaag tcgctttgat gaacttgagt tctatctaca gctccttgcc tactactaaa
    46861 agcaaacaaa aagctggact aatacgaaaa aagctagtaa tactcaatga aaatcaaaga
    46921 gcaaactagg aaactagccg caggttgctc aaaacactgt tttgaggttg tggatagaac
    46981 tgacgaagtc agctcaaaat actgttttga ggttgtggat agaactgacg aagtcagctc
    47041 aaaacactgt tttgaggttg cagatagaac tgacgaagtc agtaaccata cctacggcaa
    47101 ggcgacgttg acgcggtttg aagagatttt cgaagagtat aaaggtgtat cgaaataaaa
    47161 atctcctcat caatatctct ggtcgtggag gcaaggacct agctgcgatt gcagattacc
    47221 tagaagctaa aaaataatag atggaaaaat tacagtcttt tctctcacag agagcttgtg
    47281 gttgctgaaa acaagcagag aaagctgtaa agttgggttc atctgaaacg agagaagaaa
    47341 acatcattct caagggagtg ccctttatcg cacagcctgt tacaggaggt atctgagaca
    47401 ggaatgagag ataggagaaa tcctataatt gaggtggcac cgcgaatttc gtcctcacgc
    47461 aagttatttt gcgtggggat ttttcataca gtcaccacgg actagaagta gaactgaaag
    47521 ggaaattaca atggaacgaa tcattcatgg agatgtctta tcaccaatct tggcttatat
    47581 gcgcttaaag gggcaacaca aggttatctt agagagtatt ccgagagaca aggaaacagc
    47641 tcgtttttct atcttagcct ataatcctgt ttttgagatt aagtttgaaa atggagttct
    47701 ttatcaaaat ggtcaagtga ttgatcgaga tcccttggat ttcctttatg aagtgattca
    47761 taagagtcag caccactcag agctaccttt tggtggtgga gccattggtt ttgtgggtta
    47821 cgatatgatt tcgctttatg aagaaattgg tcaaatccct gaggatacaa ttgggacgcc
    47881 agacatgcat ttctttgtct atgagagcta tatggtcttt gaccacaaga aggagaagat
    47941 acatgtcata gaggatgctc tctatagtga gcgcagtcaa gaggccttgg aaaaatcctt
    48001 gaaccaagtg cttgaggaat tacgcattcc tgctccaaat gaatttgaag acttggattt
    48061 atcaccactt gacttcaaac cgcatatcgc tccacataag tttgaggtaa tggtagaaac
    48121 agctcgtgac ttgattcgta atggtgatat gttccaatgt gtactcagcc agcgtttctc
    48181 agcagaagtt actggaaatt catttgactt ctacagaaat ctccgcgtga ccaatccatc
    48241 caattacctc tatttctatg attttgggga ttatcaaatc atcggagcta gtccagaaag
    48301 tttagtttct gtcaaaaatg gcatcgtgac aaccaatccg attgcaggga cgcgaccaag
    48361 aggggttacg gatgaagaag acaagacctt ggcgactgac ctcctctctg atgagaagga
    48421 aacagcagaa catcgaatgt tggtagactt ggggcgtaac gatattggcc gcatctctga
    48481 aacggccagt gtccaagtta ctaagtatat gaaggtggag ctcttccgct acgtcatgca
    48541 tttgaccagc gtggtcaagg ggcgtttgct tgcagaactc actgccatgg atgccttgaa
    48601 agcaacgctt cctgctggaa ccgtttctgg agcaccaaag attcgggcga tgagacgcat
    48661 ctatgaactg gaaacggaaa aacggggcgt atatgcagga gcaatcggct acttgtctgc
    48721 gacgggtgat atggatttgg ccattgccat ccgaaccatg attctcaaaa atcaaagagc
    48781 ctatgtgcag gctggggcag ggattgtcta cgactctatc gcccaaaacg aataccaaga
    48841 aaccattaac aaagcaaaat ctatgactag aattggagaa ctaagaccat gattttattg
    48901 attgacaact atgattcttt tacctataac ttggcccaat acattgggaa ttttgcagaa
    48961 gttcaggttc tgagaaatga tgattccaag ctgtatgaag aagctgaaaa agcagatggt
    49021 ctggtctttt ctcctggtcc tggttggcca gttgatgctg gaaagatgga agacatgatt
    49081 cgtgattttg caggcaagaa gccgattcta gggatttgtt tgggccacca agccattgca
    49141 gaagtctttg gtggtaagct aggtttggct ccaaaagtca tgcatgggaa acagagcaat
    49201 atcaactttg aagcgccatc tgttttgtat caaggtattg aggatggacg tgcggtcatg
    49261 cgttatcaca gtattttgat tgaggaaatg ccagaagact ttgaagtgac agctcgttcg
    49321 actgatgacc aagccatcat ggggattcaa cataaaaacc taccgattta tggcttccag
    49381 taccatccag agagcattgg aacgccagac ggcttgtctt ctattcggaa ttttatcgag
    49441 aaggttgtaa agtgaggaaa ctaggatgaa agagattatt gaaaaactag caaaatttga
    49501 aaatttatca ggtgtggaaa tgacggatgt cattgagcgt atcgtaactg ggcgtgtaac
    49561 tgaagcgcag attgcttctc tcctcttagc tcttaagatg aagggggaaa cacctgaaga
    49621 acgcacagcc attgcccaag tcatgagagg acatgcccag catattccaa ctgagattca
    49681 tgatgccatg gacaactgtg gtacaggtgg ggacaagtct ttcagtttta atatttccac
    49741 aactgcagcc tttgtcttgg ctggtggcgg tattcacatg gccaagcatg gtaaccgctc
    49801 gatttcttct aaatctggtt ccgcagatgt cctcgaagcc ttgggaatca atcttgacct
    49861 caaaccagct gaactaggta aggtctttga taaaactgga atcgtctttc tcttcgctaa
    49921 aaatatgcac ccagctatga aatacatcat gccagctcgt ttggaattgg gaattccaac
    49981 gattatgaac ttgactggtc cactgattca tccaatggct ttagaaacac agcttcttgg
    50041 aattagtcgt ccagaactcc tagaaagtac agctcaggtt ttgaaaaata tgggtcgcaa
    50101 acgtgccatc gtggttgctg gaccagaagg gttggatgaa gctggcttga acggaacaac
    50161 caagattgca cttcttgaaa atggcgaaat cagcttgtca agctttactc cagaggattt
    50221 gggaatggaa ggctatgcta tggaagatgt tcgtggtggg aatgctcagg aaaatgcaga
    50281 aattttgctt agcgttctga aaaacgaagc aagtccattc ttggaaacga cagtcttgaa
    50341 tgctggtctt ggtttctatg ctaatggtaa gattgatagc atcaaggaag gagttgcctt
    50401 ggcccgtcaa gtgattgcta gaggcaaggc ccttgaaaaa ctcagactgt tacaggagta
    50461 tcaaaaatga gtcaggaatt tttagcacga atcttagagc agaaggcgcg tgaggtggag
    50521 caaatgaagc tggagcaaat ccagcctctg cgccagacct atcgcttggc agaatttttg
    50581 aagaatcatc aggaccgctt gcaggtaatc gctgaggtca agaaagctag ccctagtttg
    50641 ggagatatca atctcgatgt ggatattgtg caacaggccc agacttatga agaaaacgga
    50701 gcagtgatga tttcggtgtt gacagatgag gttttcttta aagggcattt gaattatcta
    50761 cgggaaattt ccagtcaggt agagattccg acgctcaaca aagactttat catagatgaa
    50821 aagcaaatca tccgcgctcg caatgcaggt gcgacagtta tcttgcttat tgtggcagcc
    50881 ttgtccgaag aacgcctcaa ggaactgtat gactacgcga cagagcttgg tctggaagtc
    50941 ttagtggaga ctcacaatct agctgaacta gaggtagccc acagacttgg tgctgagatt
    51001 atcggggtca acaaccgcaa cttgactacc tttgaggtcg acttgcagac cagtgtagat
    51061 ttagcccctt actttgagga aggtcgctat tacatttctg aatctgccat tttcacaggg
    51121 caggatgcgg aacgactagc cccatacttt aacggaattt tggtagggac agctcttatg
    51181 caggcagaaa atgtggccca gagaatcaag gagttgcaga ttgacaaagg ttaaaatttg
    51241 tggactatcg accaaagaag cggtggaaac agccgtttca gcaggagccg actatatcgg
    51301 ttttgtcttt gcacctagta aaagacaggt gactttagaa gaggcagctg agttggcaaa
    51361 gcttattcct gcagatgtga aaaaggttgg agtatttgtt tcaccaagtc gggtagaact
    51421 gctggaagcg attgacaaag ttggcttgga cttggttcaa gttcacggtc aggtggcagc
    51481 tgatttattt gagaatttgc cttgtgccag tattcaggct gtgcaggtag atggaaatgg
    51541 gcatgtcccc aattctcagg cagattatct actctttgat gcccctgtgg caggaagtgg
    51601 tcagtccttt gattggggtc aactggatac gactggacta gcacagccct tctttatcgc
    51661 aggtggcctt aatgaagata atgtagtaaa agcaattcaa cattttactc cctatgcagt
    51721 agatgtatcg agcggagtgg agacagatgg acaaaaagat catgaaaaga ttagaagatt
    51781 tatagagagg gtaaagcatg gcatatcagg aaccaaataa agatggattt tacggaaaat
    51841 tcggcggacg ttttgtccca gaaacattga tgacagcagt tttggagttg gagaaggcct
    51901 accgtgaaag tcaggcagac ccaagtttcc aagaggaatt aaaccagctt ttgcgccagt
    51961 acgtaggacg tgaaactcct ctttattacg caaaaaactt gacccagcat atcggcggag
    52021 ccaagattta tctcaaacgg gaagacctta accatacagg agcccacaag attaacaatg
    52081 ccttgggaca agtttggctt gccaaacgca tgggtaaaaa gaaaattatc gcagaaactg
    52141 gtgctggtca gcacggtgtg gcaactgcaa ctgctgcggc cctctttaac atggaatgta
    52201 ccatctacat gggtgaggaa gatgtcaaac gccaagccct caatgtcttc cgtatggagc
    52261 ttttgggagc taaggtcgag gccgtgacag atggttcgcg cgtgctcaag gatgcggtca
    52321 atgcagccct tcgttcatgg gtggctaata tagatgatac ccactatatc cttggttctg
    52381 ccttggggcc tcatccattt ccagaaattg ttcgtgactt ccaaagtgtc attggtcgag
    52441 aggctaaaca acagtaccgt gacttgacag gacaaaatct gccagatgcc ctagtagcct
    52501 gtgttggtgg tgggtcgaat gccatcggtc tcttccatcc ctttgtagaa gatgagtcag
    52561 tagccatgta tggagctgaa gcggctggat ttggtgtgga tacggagcac cacgcagcta
    52621 ccttgaccaa gggtcgtcca ggtgtccttc acggttccct catggatgtg ctccaagatg
    52681 cccatggtca aattcttgaa gccttctcta tctcagcagg tttggactat cctggtatcg
    52741 gtccagagca ttctcactac cacgatatta aacgtgccag ctatgtccct gtgacagacg
    52801 aagaagcctt ggaaggattc caactcttgt ctcgtgtgga agggattatc ccagctttgg
    52861 aatctagcca tgctatcgcc tttgcggtga aattggccaa agaacttgga ccagaaaagt
    52921 ctatgattgt atgcctatca ggtcgtgggg acaaggatgt ggttcaagta aaagaccgct
    52981 tggaagcaga tgcagcaaag aagggagaag ctcatgccta agacactaac aaaaaaatta
    53041 aatgctatta aagcagctgg aaaaggaatt tttgttccct atatcatggc tggtgaccac
    53101 gaaaaaggtt tggatggtct tgctgaaaca attcactttt tagaagattt gggtgtctct
    53161 gcaattgaag tgggtattcc cttttcagac cctgttgcag atggacctgt tatcgaagaa
    53221 gctggcttgc gcagtttagc ccacgggacc tctacccagg ctttagttga aaccttgaaa
    53281 accattgaaa cagagattcc actggtcatc atgacctact tcaaccccct ctttcagtac
    53341 ggtgtggaga actttgtcaa agatctggct gatacagctg tgaagggctt gattatccca
    53401 gacctgcctc atgaacatgc caactttgta gaaccatttt tggcagatac agatatcgcc
    53461 ttgattcctt tagtaagctt gaccacagga attgagcgcc agaaagagtt gattgaaggg
    53521 gcggagggat tcgtctatgc cgttgccatc aatggggtaa cagggaaatc aggcaattac
    53581 cgtgcagatt tggacaagca cttggcgcaa cttcatcaag tggccgacat cccagtcttg
    53641 acaggttttg gtgtatctag tcaagccgat ctggaacgct tcaatgcggt gtcagatggt
    53701 gttatcgtcg gttcgaaaat agtaaaagct ctccaccaag gagagccgat tcaggacttt
    53761 atcaaacaag cagtagctta ccaaaaataa ttaaacaagt agcaagtaag aggaaaggca
    53821 cgaaaggaag tctttccttt ttcttttgca ggagaaaggc tagttttata gtgaattgaa
    53881 tctggaatat tacactacgg ttcctaaaat attttaagaa agtgatttga atttcccaat
    53941 aaatttgttc agtttttaat tgattttact atagaagata taattttcta aattactctg
    54001 acacagaact ttaagaaaaa gctaattatt gtagtaattt ctttaagaaa atgatataat
    54061 tcattaaaaa gtatttggag gaggaagaat tatgttgaat caggatctct ttgattcgct
    54121 tgaggcacaa aaaattgtag atactttgat gaaagggcaa aaagattatg tagatgaacg
    54181 tctagaaaaa agagagacaa tgatagtgtc gaatggttat gcatggacgc gacctaatca
    54241 tattgatact gcatttgcat cagcagattt gtttgagtat aaattacaat tagcaggaca
    54301 gacttgggga tatttagaat ttgaaacaaa tacagaaaaa tatgggaaag tattgttaat
    54361 tataaagggt aagaagcgac ttacgaacca atttcctttg gtacaaaaaa ataagagtgg
    54421 ctacttattt gaatatgctc aaatgaatac actttatctt aatcaacatt cttcctacaa
    54481 aaatgatgaa gatagtcatt cctttccaat tcagatggag ttagtttctg atgaaatgat
    54541 tcaagaaatt gaacaagcta ccaaaaattc gaatatcgaa aaatttatga ttttaactta
    54601 tgaggcggac tcagaaaaca atattatatc tgtagatgtt gttatgcctg atgcacgaac
    54661 tggtcagtta cacttgattc aagatttgtc tgagtatatt caatcaagtt cgtaccattt
    54721 cgaagaagct aaataccaag atattcctaa tttttcagaa ttatcggaaa cagaagattt
    54781 tgaaattatt ccaagaatag aaaaacaaga aggtcaaaag tagtaaggag tatgtaaatg
    54841 tttaatggtc gggtattgaa agaattacgg ctgttaaatg gtttaagtag agcagaatta
    54901 gctcagagaa ttaatttaac ggaacaagcc atttggcagt ttgagtccaa cgaaacgaaa
    54961 cctaaattat caaccaaaat gcatttggcc aaccaatttc atgttgattt aacttatttt
    55021 gaacaggaag aagagagcat tcgatttgat tcttctgtaa ttgcctttag aaatgcagac
    55081 ctagcaacac ggaaaacaat agatattcaa actatgtatt tacataaggt agatagtttg
    55141 attgattatt ttgaaagttt tgtaattata cctaatatta caattcatga cctaagtaat
    55201 gtagtgagtg aatcttatca taagggagaa tccattgagg aattggctct ttatgccagg
    55261 gaaaaattag gtatttcaaa agataatcat gatttgcttt ataaattaga acgttcaggc
    55321 atctatatcg tggaacgatt aattaatggc caagctgatg cttatagcgc atggtcaaaa
    55381 ttgggaagac cttatattgt gttgggaacg aataaatcat ctgtacgtcg aaattttgac
    55441 ttagctcatg agctaggaca tattctttta cataaatata aagatatgaa tgaagatggc
    55501 gatcgtttgg agcaagaagc aaattatttt gcatcatgtt ttttattgcc aaaagaagag
    55561 tttttagtca aatttgaaga gagggttggc aagcgtgtca gcaatcctga tagttatatt
    55621 ttattgaagt cggatttgaa tgtttcgata caggctttag agtatcgagc ttttaagtta
    55681 ggattattga ctccaaagca acattcttac ttttatcgtc aaattgcgca aaaaggttac
    55741 aaaatgattg aacctttgga tgatcaaatt tttgttaaaa aaccaagcaa agtaaagagt
    55801 attctggacg tcgttttgag taatcatcta gtcagtctag cgactataat gtctaaacaa
    55861 agtattcgtt tacagtttat aagcgaaata ttttcagtcg aaatgaaatt ttttgatcag
    55921 tatcaagaag atagaagaac agatcgattt gataacatca tccctttgta caaaagaaat
    55981 aatttataat ttaactgtga aataatttca tgaaggacat atggcagaaa ttggatttta
    56041 ttagacaagc agtagcttac caaaaataat caaacaagta gcaagtaaga ggaaaggcac
    56101 gaaaggaagt ctttcctttt tcttttgcag gagaaaggcc aggatacccg tcgcagaagc
    56161 gaactgaatc aagatcagta actccgttac gctaaagacg agagcacaag aagctaaaaa
    56221 aaggaaatcc cctgcaccca tgcggatatc gataaaatga gccaaaattc caagtatgag
    56281 gaaggagacc atgaccagat tccagccaga ggaagctatt aggattaggt ggcaagtcat
    56341 ccagaccagt aagggatatt cctgatggtg aaagtcgtag atacccaagg tcaaaccagc
    56401 ggtgattagg acgacttgcc ccaaggaaag caatttccaa gagtaaagca gaaagaggag
    56461 tcctaagact aattcaaaga gggcatacca gacaggatag cgaactttgc agtagcgaca
    56521 gcgaaagcga ttgaagacct gtgagagaat cggaatcaaa tctaagggac gcaagcgagt
    56581 ctgacaggaa tcgcagtgac tggctgaact gataatggat tgctctggaa aacggtcaat
    56641 gaccaaacca agaaaggaag cgagaatgct cccgacgaga aaaaaataaa aatcaatcat
    56701 acttatctat tcgtaaaaaa tgggagaaat agtataatgg agaaacatag ataagatggt
    56761 gaggaaaaga tgacaattcg ttttgaagaa aaggtgagta tagaaaacgc tcaatttgta
    56821 tgccagtggt ccaactccct aggcaaatcc tttcaagaac aatggatggg accaaggatt
    56881 ccttttctac tgacccttca agccttggaa ggagtctttt caatctttga tgaacaagag
    56941 tttgtgggat ttatccagaa aatcaggcta gaagacagca atcttcatat cgggagattt
    57001 tttatcaacc cccagaaaca ggagcaaggc ttaggtagcc aggctttaag gaaatttgtt
    57061 agtttggcct ttgaaaatga agatatagat agtatttctc taaatgtctt cgaggcaaat
    57121 caaagagctc agaatcttta ccaaaaagaa ggatttgaaa tcgttcaaat ggttgaagca
    57181 cctgtacgaa aatatagtat attgaaacta gaatagtaca cctctacttc taaaacattg
    57241 ttagaaatcg atttgactgt cccgatcgat tcgtcctgtt attatttcat tttactatac
    57301 attatgaaaa aggggagata ggagtataat tccaaatata aagaaagcga acaaaacaga
    57361 attctgattt tcagaaaaca ggtttgttcg ctttttatga tatactccct ctttttagcc
    57421 tatttattaa atgtaataga aaattaggaa tgtagaattg tatgatacaa acaaagtgca
    57481 atagctgagt gtgcggctgt aaaatcattg taaggttcaa taatgacttc aggtaggcga
    57541 ttatagagca gtttgggttt gtagttcatt tcactatata gtttttggat gatgtgttga
    57601 tttgttagta aaggactatg taggtagatt ttttgagagt caatcatcat actgatattt
    57661 aaaatggttt gactaagata gagtaaggct tgatggataa gtgtgattat tccagtgtca
    57721 cctaattgat aagcggttaa aataacttgg atatcaatat catcggcatt tttgactaaa
    57781 ctaggaagta gagaataggg agattgatga tatagaattt ttgatttttt gattaaccaa
    57841 gattctccag caaaagtttg taagcatccc ttgcgcccgc aactacattc ttctccctca
    57901 gatgaaacaa ctgtatgccc tatttctcca atcattagat ttccttttcc ataaatgttt
    57961 ccgtcgtaga tataagaaca gtgcatacct ctagcaaaat gaaaataagc aaaattaggg
    58021 tcattctgtt ggcgactaaa taggcgttta cctatagcca tacagttaac attgttagag
    58081 aaaaacaaag gcttatcaaa gtgggattga atcatttcta agtcgatatg ttgccataaa
    58141 ggattgtttg ttgttatttt ataatcatct aaatagcgac caggtagcgc gattccaatt
    58201 gcttctattt cataatcaga gcagttattt aagaattgct ttagtgtctg gctaatgagt
    58261 tgatttccct tttcttggat taactgttta gtaacaattt ctttctcttc ctttaagata
    58321 tttcctagat tatctcctaa agcaaaagtg aaatgttttt cagatagttc acaaccaatg
    58381 tagtaaaatc gtttagcttg aatgtctagc aggatttttt ttctccctac gcttgtatcg
    58441 tgttcgtctt caccaagttc taagagaatg ttttctttaa tcagatcatt tgtgatactg
    58501 ctagttgttg caggtgttat tcctgtttta gtagcgattt caattcgtga aatgggacct
    58561 aatgtgtaaa ctgtttccag tattttagct cgtagttgta attgtttctt agataaggtc
    58621 atattagttt tcttcctagt tgtcagattc ttaaaatcct atgatttttt gctatggata
    58681 aatgtagatg ttaatctttt taagcttttc attgcttact ggtctctttt tattatatat
    58741 tgataataaa aaataatcaa atatttacac aaatccttga caaggctttc ttatagttgt
    58801 aaaatataaa taacaaaaga taatcaaata acaacttgac gtcaaaaaat aaagataatt
    58861 gataactttt gataataaaa tatatttaag aggaaaaggt tatggaaaaa ttattacaag
    58921 aaaagttact tccagtagcg gctagattgg gtaataataa agcgttggtt tctattcgag
    58981 acggtattac tctaactata cctttattgt tgataggttc attactaatg gttattgcaa
    59041 gttttcctat tccaggatgg gaaaaatatc tgggtgatat tggtgttgct gactatttat
    59101 ggaagggtgt tgatagtagt tttggattac ttggtcttgt agctagtttt ggtatcgctt
    59161 actttatggc tagacaatac aaagttgatg gtattcctgc tggtattgtg tcattatcat
    59221 cttttattac agttacgcct ttcattagag gagaggctgg agcaggaatg cctaccgctt
    59281 tcatggcatc aaaaggttta tttgttgcta tgattttagg attgattaat ggctatatct
    59341 accaatggtt tataaatcat aatatacaga taaaaatgcc ggatggtgtt ccaccagcag
    59401 tatctaaaag ctttagtgcc attattcctg gtgcagtgac tattgttggt tggttgattg
    59461 tttatgcaac tttagataaa ttaagtttac ctaatcttca tgaaattgct caagtagctt
    59521 tgggaggtcc acttggactt ttaggaaata atgttattgg tcttcttatc ttaattttcc
    59581 ttaatagtag tttttggttt gtaggattac acggaggaaa tgttgttaat gcagttatga
    59641 aaccgttatg gttagctaat ctagatgcaa ataaagtagc ttatcaaact ggagaaactc
    59701 ttccaaatat ttttactagc gtatttatgg ataattttgt attcataggt ggcggtggtg
    59761 caaccatagg tttagttctt gcgttaggat atttagcaca taagaagaaa gctagtaaac
    59821 aactaaaaac tttggcacct ataactgtta tacctggatt atttaatata aatgaaccag
    59881 caatgtttgg cgttcctatt gttttgaaca ttttgttact agttccattt atcttagctc
    59941 caatgtttaa cttactagta gcgtggggag caatggcatc aggtttggtt ccgctaactt
    60001 atacagatcc aggttggact atgccacctg ttataagtgg tttacttgct acaggaagta
    60061 tttctggttc attattacaa attgtattga ttgttttgga tgtcttactt tatttaccat
    60121 ttgtaatagc tattgaaaaa cggtttaagt tattggagga ttgagaatga aacgtttaat
    60181 tagtgcaaat ccatctgaga tattacagat gaatgctgaa gaattaaaac aaagtatttt
    60241 agcaagtgaa ggtagagttg ttctatctga aaatgtagtt actcgtgaga catttgttgg
    60301 ggatataact aattctgaaa ttgctagagc ttttggagct gatatgattt tattgaattg
    60361 tgttgatgtt tttgagccta aaatttatgc tttggatagt tcaggtgatg atgttattca
    60421 tcgcttacac cagcttgttg cttgtccaat tggtgtaaat ttggaaccga ttgacccatc
    60481 tgcaaagatg ctagaggaaa cacaggaaat tgttgcaggt cgtgttgcta gtgttgaaac
    60541 attgaatcga atagaggagt taggttttga ctttgtctgt ttgactggaa atcctggaac
    60601 aggagttagc aatcgagaaa tcattaaggc tgttcaaact gctaaggaaa acttttctgg
    60661 tttgattatt gcaggtaaga tgcacggggc aggagtgaat gagcctgtgg cagagctttc
    60721 tgtagcagag caattgttgg aagcaggtgc ggatgtgata cttgttccag cagttggaac
    60781 cgttccagcc tttcatgacc aagagttgcg tgaagtcgtt gatctcgttc atagtaaggg
    60841 tgggctagta ctgagtgcta ttggtactag ccaagaaaca tctgatacag atactatcaa
    60901 ggaaattgca cttagaaata aaatttgtgg agttgatatt caacatatag gtgacgcagg
    60961 atatggggga ctggcaacag tcgataatat ttatgcattg agcaaggcaa ttagaggagt
    61021 gagacataca gtatctcgct tggctaggtc agtaaatagg tgataaagga gtaagctatg
    61081 gctaaagtaa caattatgtt agcatgtgca gcaggtatga gtacaagtct gctagtgaca
    61141 aagatgcaaa aggcagcaga agataagggg ttggatgcag aaatttttgc agttccagct
    61201 cctgaagcag aagaaattgt agcaacaaag gaagtaaatg tgttgctttt aggccctcaa
    61261 gttcgctatt tactagggga ctttcaagaa aaactaaaag atagacagat tcctgtggcg
    61321 gttattccga tgacagatta tggaatgatg aatggttcta aagtcttaga tttagctgaa
    61381 agtttattag actaagattg aggagaatgc tatgaatgaa agtaatttag aatctgcaat
    61441 ggggctaatt atgtatggtg gggaagccaa aagtaatgct atggaggcta ttcaggcagc
    61501 aaaaaaaggg gatttctcta aagccaatcg aagattagct gatgcgaatg ctgccttatt
    61561 acaggcgcat aaggctcaaa cagaaatgtt gacaagagag gcacaggggg aagaaacttc
    61621 aattagcctc ttgatggtac acgcgcaaga tcatttaatg acgagtctga cctttgttga
    61681 cttggcgaaa gaagtggtag aagtatacga acgatttgaa aaaaattagg aatttgctat
    61741 gaatacaatg ttggataaaa tgcaagaaaa actttctcca attgcaatga aagttgggaa
    61801 tcagaagttt ttagttgctt tacgtgattc atttgtgggg actatgcctg ttattatgac
    61861 aggttccatt gctttgttat taaatgcttt tctagtggat ttaccacaac aatttcacct
    61921 agaaagtatt acgaaaacct ttcagtggct tgttgacatc aataatttgg tattcaaggg
    61981 aagtatacca attgtttcct tattgttcat ctattgcttg ggagtgaata tcgctaagat
    62041 ttacaaggtt gatacagttt ctgctggttt ggtatcactt gcttcgtttg tcatttcgat
    62101 tggaagtaca gttacaaaga gtttcccttt ggcaaatgta ggagatgtga aattaaatca
    62161 aatcttacaa ggaattgata atctagcatt tgatggtaaa aatctgatgg taactattgg
    62221 gaatgtgatt ccaggaaacc atattaatgc cagaggatac tttacagcca tgatgattgg
    62281 atttttagcc tctattattt tctgtaaagt aatgaaaaag aactgggtaa tcaagttacc
    62341 agattcagtt ccccctgcga ttgctaagcc gttcacttct atcattccag gatttatggc
    62401 gatgtatata gtagccatct tgacttatgt tttccattta cttagcaatg acttattgat
    62461 tgattgggtt tacaaggtgt tacaaacgcc attactaggt ttgtctcaaa gtttctttgc
    62521 agttattctg atgatctttt tgaataagtt attctggttt tttggtctcc atggaggaaa
    62581 tgtattggct cccatcatgg aagggctatt tggagttgct atgttagcga atctggatgc
    62641 tttccaaaaa ggtgaaccga ttccttatat atggacaagt gggtcttttg gggcgtttgt
    62701 ctggtttgga ggattaggat tagtacttgc tattttaatt ttttcaagaa acagtcatta
    62761 tcgaaaagtt gctaaacttg gtctagcacc agttctgttt aatattggtg agccggtcaa
    62821 ttatggttta ccagttgtct tgaatccttt gctatttata ccatttgtac ttagcccggt
    62881 tttcatggca acggtagctt actgggcaac aagctggggt cttgtttcac ctgttacaca
    62941 aaatgttact tgggtaatgc ctccaatttt atatggtttc ttctctacag catttgattg
    63001 gcatgccatc atcttatcag ttgtttgttt gattattagt gtcttgactt atttcccatt
    63061 tgtgaaaatg gcagataaaa ctgaattgag ttaatggtct tacttgcgtt ttttgtgata
    63121 aaatagaaat agattacgtg taagatgagt taggtgtagg atttcttatc tccactaaaa
    63181 ttgttaatag acaaaggttt atagtttatt gaagttggaa tagtacactg taacttctaa
    63241 aacagtacta gaaaatgatt tgaatttcct aatcgattta gtcatatttt agttcatttt
    63301 tatataaaac ataacaagaa tgttattgtt ttatatagag agattgatat agaaaggggt
    63361 gcagtaaagg tagatatctt cttgtgcctc ttttgccata ttagaaagga gattttatgg
    63421 ttcagacaaa acagccaaat attattttga ttgttgtaga tcaaatgaga gcagacgcct
    63481 tatccttgaa tagtaaggat aagctagtta gcactccaac attagatatg atggctagtg
    63541 taggttataa ttttgaaaat gcctattctc cagttccttc ttgcgttcct gctagggcag
    63601 ctttattgac tggcttagac caagataaaa gtggacgtgt tggttatcaa gatgaggtgc
    63661 cttggaattt tactaatact cttccaaaag tctttaaaga tatgggttat caaacagagt
    63721 gtattggtaa gatgcatgtt tttccttcca gacagagact tgggtttgat catgtcttac
    63781 ttcacgatgg gtatctacat gttgatagaa agtatgataa ggcttatgga agtcaatttg
    63841 attatgctag tgactatcta gcctttttaa agggtaaagt tggttatgat gttgatttga
    63901 ttgatgacgg aatggattgt aattcttggg aagctagacc ttgggataaa gatgaaaagt
    63961 tacatccaac aaactgggtt gtgagtgagt ccatttcatt tttacaaaga agagatccaa
    64021 cggttccttt ttttctcaaa atgtcttttg aaaaaccaca tgctccatta aatcccccaa
    64081 aatactattt tgatatgtat atggaacgct tgcctcaatt tctcgatttg catatcggga
    64141 attgggaagt attagaaaag caaattccgt ctatttatgc tttaagaggg aaattaaagg
    64201 aagatgatca aagaagaatg gttgctgctt attttggatt gattactcat atagaccatc
    64261 aaattagtcg ctttttgact gccctaaaag aatttcgtca tgataaagat acgattatct
    64321 ggtttgtttc ggatcatgga gatcagcttg gtgaacatta tctgtttaga aagggatatc
    64381 cttatcaggg aagtatccac attccttcgt ttatttacga tccggctgga ttgatagctg
    64441 gtaatagggg aacaatcaag caattagtga aaattcaaga tatttttcct tcactcgtgg
    64501 atttggcagg aggaactact acagatgaac tagatggacg gagtgttaag aatttattat
    64561 ttggacagta cgaggggtgg cgaacagaat ttcatggtga acatgcttta ggaaaagatt
    64621 ctagtcaata tattttgaca gaccagtgga aatttatctg gtttcctgtt ttaaatcatt
    64681 atcagttatt tgatatgaaa aaagatccgc atgaaatgaa tgatttgtat ccaagtgaaa
    64741 aataccaacc tattgttcgt cagatgaaga aaaaattagt aaactttctt agatatagag
    64801 aagaaggttt tgttgtggat gaagagttag ttcctgttga actttctaaa ataacgccaa
    64861 ctctaacgaa aacgggggat agtcaatcat gaaaatgaaa aaaatcaagg gtgggacatt
    64921 tatgatggga acaaattctg aagagggctt tttagataat tttgaagatc ctcaagtcgc
    64981 tgttagtgta aaggattttt ccattgcgga tactccagtt acgaatcaag aatttgctca
    65041 atttgtgaaa gaaacaggct ataaaacact agctgagcga caagagtggt cttttgtttt
    65101 tatattgttc gttccggagg cagaaaggga gggatatcct catcctgccg gggctccgtg
    65161 gtggttacaa gttccgaacg cttgctggaa acatccctac ggtgagaaca gtaatctagt
    65221 gggtttagaa gaccatccag tagttcatgt tgctttagaa gatgcattgg ctttctgtaa
    65281 ttggagcgga atgtctttac cgactgaagc acagtgggaa tacgctgcta gaggaggaag
    65341 acaatctgaa tatccttggg gagatactct tttagaaggt ggatattatc atgccaatac
    65401 ttggcaagga agatttcctt atgaaaatac tggcttagat ggatttatag gaacggctcc
    65461 tgtctatgaa tttttaccta atgattttgg tctatatcaa atgattggga atgtttggga
    65521 atggtgtcgc aatccgagat ataccttatt agctagtttt aatgaagatg actatgagtt
    65581 gccaaaatat gggatacaag atgaggagta tgctatcaga ggaggttcct ttttatgtca
    65641 ttgttcttat tgtaatcgat accgagtagc tgctagaaat ggatgtattt ctacttccac
    65701 tagtagccat ctaggtttta gatgtttgaa agaataggat ggacttaaaa agtggttgag
    65761 attatttatt ttctaatcat tataatagct agtggattgg gttctatatc aggcatgggt
    65821 ggtggaatta taattaagcc acttatggat agttttggat accattcggt cagtgatatt
    65881 gctttttatt ctagtttctc tgtttttata atggcgatta tttcaacaac caaaagattt
    65941 tcacaaagta aagagataaa atggagattg atttttacgg tatccttttc ttctgtttta
    66001 ggaggatttc taggtcatct tatttttcaa gttttgttat ctcaattatc agtacggcta
    66061 gtatccattg ttcaaatgat tttactattt gtgatgctac ttgtatcatt tgttttaaca
    66121 gattttaaaa aaacttatca atttgataag ataggattct atatgatttg tggtcttcta
    66181 ttgggcttga tttctagttt tttagggatt ggtggaggtc cgctcaatgt atctttattg
    66241 atggtgtttt tttctatttc gataaaagaa gcaacgatgt attctcttgc aattattttc
    66301 ttttcccagc tgtctcattt ggctacaatt gttgtggtta caggtttaaa tcagtatcat
    66361 ttagcacctg ttccagttat ttttcttgct tctatatgcg gaggggtatt ggggacggtt
    66421 gtatcaaaag tcttacctga aaattgggta agatactgtt ttaaagggat gttattcttt
    66481 gtcatgggaa tgactttata taatttattt catattttgt aaggtcagtg tgaaaatatt
    66541 taaatagact tcctgtaaaa actggattta acgccctcca tttgtcaaat taagctagac
    66601 agtcttacca actcagaaaa taacctgatg aggtatttgg tatccaaagg atttaggtgg
    66661 gatgtgattt cttgaggctg ggaaaaagtc tttaaaatta aaaaatgcat aatatcaggt
    66721 gttggaaaaa ctttgatact atgcgtttta ttgtgggaat atttatttca ttttctcctg
    66781 aaattgagtt cttgcccagc ctaaaattct tgactgactt cgttgaaatc tgtctgcttt
    66841 ggcaagtcat gacaccttaa aagtctattt gaatgttcgt tgagacaccg ctatcttgga
    66901 catcctagat tcgcaaaaaa gctctcacta tcgtgatgat tggaaatatc tttccatttt
    66961 gaaaactctt ttccacagtc aaacgtaata gatttaaaga ggttcactag aaattgtgcc
    67021 agccaccctt ggatggattc tgccatatcc tttgccttac gactcttggt cttcaaactg
    67081 acaatcaatt gactatacct gttcgctcac aaataggaga gaacttttta cgaaaatcta
    67141 atgaatatgc cacaagaaca ttataccgca acgtgtacta tttttggttc attttactat
    67201 agaaactatt gaagcacctg tacgaaaata tatcatgaaa aaggggagat agaaagcaaa
    67261 cagccttgaa atcaatgttg actgcgatta tgaacctgaa tcagcccctc aaaagggact
    67321 gtatatgaat gaacgttacc agtgtttaaa aactaaagaa tatcaggcac ttttatcttc
    67381 caagggtaga caaattttcg ctaaacgtaa gattgatatg aaatctgtct ttgggcagat
    67441 aaaggtttgt ttgggttata agagatgtca tctgagaggt aagcgtcaag tgagaattga
    67501 catgggattc atactcatgg ccaacaacct gctgaaatat aataagagaa agaggcaaaa
    67561 ttaaaaagct agagttccgc aattgggaat ctctagcttt ttgttttctg agaatcatct
    67621 tgactcagac tcttttgact aatgttacta aaaagataaa gtaactttta accttattta
    67681 tcgtcgccac tcaacatgtt tttaacacct tctacagcac cttctacagc gtctttggca
    67741 tcttcggcga cttcttttac tttagaaaca actttttcag ctgctccttc tttttccatt
    67801 ttttcatcac cgatggcttt cccaacacct tctttaatag aacctttagc ttgatttaat
    67861 ttttcttcta ctgacatgat aatttctcct gttatattta ttttatctta gtttacacga
    67921 gtgttttcgt gagatactac accatttgct gcacctttaa cagcttctac accttcgcta
    67981 actttttctt gaacagttga gaaaccagat ccaagaccag atttagcttt ttcgaattgt
    68041 tctgaagtga attctcctgt tgattcagca acgtcagata cgcgatcttg aaggcttact
    68101 gagtctgctt catgctgttc tttagttttg atgtcgacaa cgtttacgtt gatttcaaca
    68161 atttccaagt cagtcatttt agcaacttct gaagatacga tttctctgat ttctgaatat
    68221 aaagctggaa catttttttg gtactcaaca ataacgttta agtcaactgc aacttgtgtt
    68281 ttaccaactt ctacgttaac accacttgtt acgtcatcgc tgttaacgat tttttcttta
    68341 agatttgaga agaaaccacc atcgattccc aaaagacctg aaacgttttc tagtgaaaga
    68401 ccaatgattt tttggataac tttatcttcg taagtaagtt cccctttgat ttcgtgagct
    68461 acaacagtag catctttctt ttctacgtta gtgtttgtgt ttttttcgtt tgacatatga
    68521 aactccttta attaaattag taatttttat ttttttattt atctatatag tttttttcga
    68581 tatataatcc agctgcaact cccagtgctc ctaaaatcaa tacaaatatt gttttgaaga
    68641 agccaaagga gacaatcaaa caagccagaa atacgcctat gagaccagcg ataattggat
    68701 atcgatattg tttaagccat tccatttttt acttccttac ttcacacgac taacagtctt
    68761 tttgttttga ggttttggtt ggtaattttt tactgcaact tcaagtttta cttgacgctc
    68821 aataccaaaa aactgcttca atccattagt tatttcattt tgaattattt ggcatctgtc
    68881 agcgatgttg tctgaaggaa gaattttacc ttctacatga acgaaacatt tattttttcg
    68941 taaatttaca tgaacagttg ggttcttgat caatctatga tcactcacca aacttctaac
    69001 aaaaccttcg attgccgaat tctttaattt taatgtatcg tttttagttt caagttggat
    69061 ttccaagtaa cgtttaggat aaaacatcac aactaaaatg gataataaaa ctaaagttga
    69121 tagaaccact gtcccccaaa agacatatct agcaagatag aattcaacta cggagttctg
    69181 tctccagcta agtagatgaa tacctagatc actaacttga tgataatcta tcaaaacagg
    69241 aaggaaaatt gtcaagatta aaatacagaa aataatgaaa catattttct ttgcttttga
    69301 catatacaat cctttcgatt taacatttaa actatttttc ccgtcattga tttaaaaata
    69361 gtttgctaac tttactggcg aaaagtttac tttctacctg taaagaatga cactacagta
    69421 atcacaatcg ctgcacctac aatagatggg agcaaagcca ttccagcgat tgatggaccc
    69481 caactaccta aaagagattg tcctgcatat gccccgacta aaccagcgaa tacatttgcg
    69541 atgattccca tagaactact tttttagtga ttttacctgc caagagacca ataagacctc
    69601 cagcaataat tgatcacaac actgcctacc ctcctttttt attttaatat caaaaaatca
    69661 atttctttga ttaatactat tatataactt taatatagaa aagtcaatta tttcgattgt
    69721 tattattata taattttaaa aaatcaaatt ctttaattgt tgctattata taatttttaa
    69781 aattaaaaat ataatcttta gacttgaatt tgattctgta ttcacgccct caatagacgg
    69841 taaaataaga aaattgttta tatattgtct ccgtagtgtt attatacgaa ataaaagatt
    69901 ttaggaaaag tcgttatatc atgctatacc tattctttgt ggtataattg caagaggttt
    69961 aatccgataa tttataaaga gagaaagata ttcatgagag atttattatc taaaaaaagt
    70021 cataggcaat tagaattatt agaattatta tttgaacata aacgttggtt tcatcgttct
    70081 gaactagcag agttattaaa ttgtacagaa cgtgcagtca aagatgatct atcccatgtt
    70141 aaatctgctt ttcctgactt gatttttcat tcttctacta atggtatacg cattattaat
    70201 accgatgata gtgatattga aatggtttac catcatttct ttaaacattc aactcatttt
    70261 tcgattttag aattcatctt ctttaatgaa ggttgtcaag ctgagagtat ttgtaaagaa
    70321 ttttatatca gttcatcttc gctctatcgt attattagcc aaatcaataa agtgattaaa
    70381 aggcaatttc aatttgaagt cagtctgacc cctgttcaaa tcattggaaa tgagagagac
    70441 attcgttact tttttgcaca atatttttca gaaaaatatt atttcctaga atggccattt
    70501 gaaaattttt catcagagcc actatctcaa ttgttagaat tggtttataa ggaaacaagc
    70561 tttccaatga atttgtcaac gcatagaatg ctaaagttgc tcctagttac gaacctatat
    70621 agaataaagt ttggtcattt tatggaagta gataaagatt cttttaacga ccaaagtttg
    70681 gattttttaa tgcaggcaga aggaatagaa ggtgttgctc agagttttga atcagaatac
    70741 aatatctctt tagatgaaga agttgtttgc caattatttg tgtcttactt tcaaaaaatg
    70801 tttttcatag atgaaagtct ctttatgaaa tgcgtaaaaa aggatagtta tgttgaaaaa
    70861 tcttaccatc tattgagtga ttttattgat cagatttcag tcaagtatca gattgaaatg
    70921 gagaataagg ataatctgat ttggcatctg cataataccg cacatctgta tcgtcaagag
    70981 ttgtttactg agtttatttt atttgatcaa aaagggaata caatcaggaa ctttcaaaat
    71041 atttttccaa aatttgtttc agatataaaa aaagagcttt ctcactattt agagactctt
    71101 gaggtttgtt ctagttcaat gatggtaaat catctatctt ataccttcat cactcatacc
    71161 aaacatttgg tgattaattt gctacaaaat cagcctaaac taaaggtttt agttatgagt
    71221 aattttgatc agtatcatgc aaaattcgtt gcagagacac tttcttatta ctgtagcaat
    71281 aattttgaac tggaagtttg gactgaatta gaattatcaa aggaatcttt agaagattca
    71341 ccttatgata tcattatttc taattttatt attcctccta ttgaaaataa gagactcatc
    71401 tattcaaata atataaacac ggtctcactc atatacttgt taaatgccat gatgtttatt
    71461 cgattagatg agtaaccaaa aataacaaaa aaacgctatt tcgcgtttga actgtacctc
    71521 aaaagttaga cagaaaaaat ctaatttttg gggtgttttt gttaagaaaa gaccttcaat
    71581 catttaagca aaatgtacat ctagatttat agccctctaa aactcttgct ttttttgtag
    71641 aagatacgaa aaaagccata aaaccaaggc ttttcctgtt ttatgtagat gtttacacat
    71701 gaaaattgaa gtattttcaa aagctaattg gagtgatttt attcatgatg ggataaatga
    71761 tctggccaaa aatgattgag aaatagttgc ttaaaaagca tttgcatgat actatatatt
    71821 gaaaggaggt gataatatgg tagaacaaag aaaatcaatt accatgaaag atgttgcttt
    71881 agaagcagga gttagtgttg gaactgtttc acgtgtaatt aataaagaaa aaggcattaa
    71941 agaagtaact ttgaaaaaag tggaacaagc gattaaaact ttgaattaca ttccagatta
    72001 ctatgctaga ggaatgaaaa aaaatcgaac agaaacgatt gcaatcattg taccaagtat
    72061 ctggcatccc ttcttttcag aatttgctat gcatgtggaa aatgaagtct ataagagaaa
    72121 taacaaatta ctcttatgtt ctataaatgg tacaaataga gagcaagact atctggagat
    72181 gttgcgtcat aataaagttg atggagtggt tgccattacc tatagtccaa ttgaacatta
    72241 cttgacgtca ggaattccct ttgttagtat tgaccgcaca tactcagata ttgccattcc
    72301 ttgtgtttca tcagataatg atgcaggtgg aagagaagca gctaaacaat taattagtaa
    72361 aggatgtcag catttagcct ttgtgggcgg acataataca accattaatg aaaccaaacg
    72421 tcgtagaatt tcatttgaaa agtatgtcca agagcataaa atacctctta gtatttttga
    72481 tttggatgag aaagttactg actattatgg aaagctagag agtttcttgg aagaaaattc
    72541 ttctatagat ggaattttta caatcaacga ttttacagct ttggatgtga ttgaaatact
    72601 agaaaaaaat ggcaaacgta ttcctcatga tgtccaaatt attggatacg atggaattaa
    72661 aatggctgga gatagagatt atctactttc aactattgaa caaccattgg aagaaatggc
    72721 aaaagaagcg gttcgtattt tgtttgatat aattgatgga aagactgtta atttgcagac
    72781 aatcctacca gtaaaatttg ttgaaggaaa aacaacaaaa aatgaaaata agtcttgaca
    72841 gaaagcgcta tcaatgatag aatgaattca gataaaaaga tttattttta aaacaaaaat
    72901 gaaacgtttc aaaaaaagaa ataaaaagac agcgccaagc gctatctttt atagaaaaaa
    72961 tgaaacgttt caaaaaaagg aggttgctat gaatagcaaa gcgaagcaag tttctctttg
    73021 ggaaagaatc aagaaacaaa aactcttgtt attgatgact gtccccggtt tagttttaac
    73081 ctttatcttt aaatacatcc ctatgtatgg ggttttaatc gcatttaaag attacaatcc
    73141 tttaaaagga attttaggga gtgattggat tggtttttct gagtttacaa aattcatatc
    73201 ctctcccaac tttggtatct tgttagccaa cacattaaaa ttaagtatct atggtttatt
    73261 gcttggcttt ttaccaccaa tcattcttgc gattatgctc aatcaactct tgagtgaaaa
    73321 agtcaaaaaa cgaattcagc tcattttata cgcaccaaac tttatctcag tcgttgttat
    73381 tgtcggtatg attttcctct tcttttcagt gggaggacca atcaacaatt ttctttctat
    73441 gtttggaatg aaggctgact tcttgacaaa tccagacttc tttagaccct tatacatctt
    73501 tagtggtatc tggcaaggaa tgggctgggc ttcaacgctc tacacggcaa cattggtaaa
    73561 tgtagatcca gccttagtag aagcagttcg actggatgga gccaatatct tccaacgaat
    73621 ctggcacatt gatattccag ctcttaagcc tattatggtt atccaatttg ttttagctgc
    73681 aggtggaatt atgaatgtcg gatatgaaaa agcattcttg atgcagacat cgttaaattt
    73741 gccaacttct gaaattatct cgacatatgt ctataaagtt ggtcttgtat caggagacta
    73801 ttcttactca acagcggttg gtttgtttaa tgcagtgatt aacgtagtat tgcttgttgc
    73861 agttaaccaa atcgttaaac gcatgaataa tggtgaagga atttaaggag gaaagtatga
    73921 aaaattcgat tatggataca aaatttgata gacgtatctt actcttaaat aaaatcatta
    73981 ttgtctttat cgttttgatg actttgcttc ctttacttta tatcgtcgta gcatccttta
    74041 tggatcctaa ggttctggtt agtagaggga ttagctttaa tccagccgat tggactgtag
    74101 aaggttacca gcgtgtattc agtgaccaat ctattctaag aggttttatc aattctctac
    74161 tatactcttt tggatttgca gctttaacag tattgctatc tgtgtttaca gcttatcctc
    74221 tttctaagaa agacttggtt ggacgtcgtt ggattaacta cttcttgatt gtgactatgt
    74281 tctttggtgg tggtttagtc ccaacttact tgctcgtaaa agaattggga atgctcaata
    74341 ctccatgggc tatcattgtt ccaggtgctg tcaatgtttg gaatattatt cttgctaggg
    74401 cctatttcca aggattgcct gaagaattag ttgaagctgc tgtcattgat ggagcgaatg
    74461 acttgcagat tttctttaaa atcatgcttc ctcttgcaaa accaattatg tttgttctct
    74521 tcctctatgc ttttgtagga cagtggaact catactttga tgcaatgatt tatatcaagg
    74581 atccaaactt ggaaccattg caacttgtac ttcgtaaaat tctcattcag agccaaccag
    74641 gtcaagacat gattggagca caagcggcta tgaatgaaat gaaacgttta gctgaattga
    74701 ttaaatacgc aactattgtc atttccagct tgccattgat tgttatgtat ccattcttcc
    74761 aaaaatactt tgataaagga attatggctg gttcacttaa aggataaaaa aagaaaaaat
    74821 aaaaggagtt ttctcatgaa attcaaaaca ttctcaaaat cagcagtttt gttgacagct
    74881 agtttagcag tacttgcagc ctgtggctca aaaaatacag cttcaagtcc agattataag
    74941 ttggaaggtg taacattccc gcttcaagaa aagaaaacat tgaagtttat gacagccagt
    75001 tcaccgttat ctcctaaaga cccaaatgaa aagttaattt tgcaacgttt ggagaaggaa
    75061 actggcgttc atattgactg gaccaactac caatccgact ttgcagaaaa acgtaacttg
    75121 gatatttcta gtggtgattt accagatgct atccacaacg acggagcttc agatgtggac
    75181 ttgatgaact gggctaaaaa aggtgttatt attccagttg aaaatttgat tgataaatac
    75241 atgccaaatc ttaagaaaat tttggatgag aaaccagagt acaaggcctt gatgacagca
    75301 cctgatgggc acatttactc attcccatgg attgaagagc ttggagatgg taaagagtct
    75361 attcacagcg tcaacgatat ggcttggatt aacaaagatt ggcttaagaa acttggtctt
    75421 gaaatgccaa aaactactga tgatttgatt aaagtcctag aagctttcaa aaacggggat
    75481 ccaaatggaa atggagaggc tgatgaaatt ccattttcat ttattagtgg taacggaaac
    75541 gaagatttta aattcctatt tgctgcattt ggtatagggg ataacgatga tcatttagta
    75601 gtaggaaatg atggcaaagt tgatttcaca gcagataacg ataactataa agaaggtgtc
    75661 aaatttatcc gtcaattgca agaaaaaggc ttgattgata aagaagcttt cgaacatgat
    75721 tggaatagtt acattgctaa aggtcatgat cagaaatttg gtgtttactt tacatgggat
    75781 aagaataatg ttactggaag taacgaaagt tatgatgttt taccagtact tgctggacca
    75841 agtggtcaaa aacacgtagc tcgtacaaac ggtatgggat ttgcacgtga caagatggtt
    75901 attaccagtg taaacaaaaa cctagaattg acagctaaat ggattgatgc acaatacgct
    75961 ccactccaat ctgtgcaaaa taactgggga acttacggag atgacaaaca acaaaacatt
    76021 tttgaattgg atcaagcgtc aaatagtcta aaacacttac cactaaacgg aactgcacca
    76081 gcagaacttc gtcaaaagac tgaagtagga ggaccactag ctattctaga ttcatactat
    76141 ggtaaagtaa caaccatgcc tgatgatgcc aaatggcgtt tggatcttat caaagaatat
    76201 tatgttcctt acatgagcaa tgacaataac tatccaagag tctttatgac acaggaagat
    76261 ttggacaaga ttgcccatat cgaagcagat atgaatgact atatctaccg taaacgtgct
    76321 gaatggattg taaatggcaa tattgatact gagtgggatg attacaagaa agaacttgaa
    76381 aaatacggac tttctgatta cctcgctatt aaacaaaaat actacgatca ataccaagca
    76441 aacaaaaact agaggttgat tatgggagat aagaaataca cagtagaaaa agccaatcgt
    76501 tttatagcag aaaataaaca tctcgttaat actcaatata agcctgaaga acatttttca
    76561 gctgagattg gttggatcaa tgatccaaat ggatttgtct attttcgtgg agaataccat
    76621 ctcttttatc aattctatcc atatgatagt gtttgggggc ctatgcactg gggacatgct
    76681 aaaagtaagg acttggtgac ttgggagcac ttgccagtgg cacttgctcc tgaccaagat
    76741 tatgaccgaa atggttgttt ctcaggctct gccattgtca aggatgatcg cctctggctc
    76801 atgtacactg gacatatcga agaagaaacc ggtgtccgcc aagtgcaaaa tatggcattt
    76861 tcagatgacg ggattcactt tgaaaagatt gaacaaaatc cagttgcaac tggatcagac
    76921 ttgccagatg agttgattgc tgctgatttt cgtgatccaa aactctttga aaaagatgga
    76981 cgctattact ccgtagtagc tgccaaacac aagaataatg tgggctgtat cgttctacta
    77041 gggtccgata acctagtaga atggcagttc gaatccatct ttttaaaagg ggtagaacac
    77101 caaggcttta tgtgggaatg cccagattac ttcgagttag atgggaaaga ttgccttatt
    77161 atgtcaccca tgcgttatca gcgtgaggga gactcatatc ataacatcaa ctcatcgctt
    77221 ttgttcacgg gtaaggtaga ttggggagaa aaatgtttta tcccagaatc agttcaagaa
    77281 attgatcatg gccaagactt ctatgcgcct caaacattgt tggacgatca aaatcgtcgt
    77341 atcctgattg cttggatgca gacatggggg cgtacccttc caacccatga ccaagaacac
    77401 aaatgggcat gtgccatgac tttacctaga attttaagat tggaagatgg caaactaaga
    77461 caattccctg ttaaaaaagg ccaatatcaa atccaaatag ataaagattg tcattaccac
    77521 ttaggaaatg atatagatta tcttgaattt ggttatgata gtaatgcgca gcaagtttac
    77581 attgatcgta gccatcttat tcaaaaaatt ctaggtgaag aagaacagga cactagtcga
    77641 cggtatgtag atattgaagc taaagaattg gaagttgttc tagataaaaa ttccatcgag
    77701 atttttgtca atcaaggtga agcaagcttg actgcaactt attacttaac ggtgccagct
    77761 gagctatcac gaattgatta aaaattaagt tatttctcct aaagaaaaag ttctctttct
    77821 aaaatagtgg aaagaggact ttttgtgttt tgggtatata agcttagttt atggtatttg
    77881 taaaattggt gttggattat gatttaagct agttttctaa agaatttgaa aaaaatttta
    77941 tttaagcaaa aaaaaccctt ggttccaagg cttttcctgt tgtatttata tgccccctac
    78001 aagtatatta tgatacatag tattaagtga ttatttattt ttgaggcttt tcttattcta
    78061 aaagtaggta tatgagttta tggacattgt aattcacaac aaattcacgg cagaaaatac
    78121 aattaggtag tttgtaattt taaaattaat gcgaaactaa ttaaatttaa aaaaaatcaa
    78181 ctttcttcct tgaccttttt aaaaaatttt ggtatactta atgggtaatc aagattgaac
    78241 aaagaatcaa gctttttcaa cttgcaaaat tatcagaaag gaggctctat aatgaggatt
    78301 caaatgagat tatctaaaga agctcgcgtt gttttagaag aattaaaatt agaatattta
    78361 aaaaaaggtg aagacaaaac atcaggagta atcgttgatg aaatttttaa acattttagt
    78421 tctaattttc aaaaagtaga ttggaaatct gttcaaagtg cacctaagtt taatgaaatt
    78481 ttggcagatt atacttcagt taatcccaca acacttaatt tatctaatag tacaataagt
    78541 acaatgtctt acattagaga ccattttaac tacatattag atatgaaaag aaccgtctat
    78601 cgttcattca taattcgcct agtcttgaag gcttacaaat tagagaagga aggcaccgat
    78661 atttatttat aatagatgtt tatttcatct attataattt tagcatgatg agtagtcaaa
    78721 actatatcta aagggaggtt aatctctctc tttattttta aaatttgaaa ggaaaaatac
    78781 aaaaatgaaa attaatgaat atttatggag aacgagctgt cttatactgc taattatcat
    78841 gagcttcttt gcaggcttcg gagctaaatt agagtttaca tcttataacc cgtttttatc
    78901 tgttaaattt ggtttggtcg tagctctgac agtctttgta gttcaattgt tagccatact
    78961 gccagccatg gttaaaagag gaaataaaaa tgagaatgtt gaatcaaaca attcgtgtga
    79021 agagtaaata cccttacttg attcgtcccc gaaatattaa aattgtaaga ttgtttatcc
    79081 aaatttttgt ctttttactc ttaatatctt cgtggattgc tataatccca tttattgctc
    79141 gtctagtctt ttccatgttt tttataagtc ttcttttttt cgtagagaag ttctttcgtt
    79201 ctaattttat agcaaaattg agaaacaagc tcaatatcag agaagccttg ttttacatgt
    79261 taattagttt aaatttatat gatgagctcg ataatgaagt agtagataca gcagttttat
    79321 attttgacgt tgagaataat aaagttgttg tctgtgcccc actatttggg aaccgttatc
    79381 tgaaaacctt gaaaaattta gaggaatatc tctgtcccac tttgggcttg tccttgctgt
    79441 ctaaaaaaga agaaatcgat aaaatagtat acgtgttagg gcagaaagaa gaaatagaac
    79501 aatatgtttt taatagtaac acattaacaa gagaattttt taaagatatt cctagttcaa
    79561 taatcaagct gtcaaataca cagaaattta gtctaaagtc caacactaac ctaggtatat
    79621 atggaagaac ggggacaggc aaaacaatag ctctgcaatg gtacttattt aacgcactgg
    79681 ctaaaggttg tggtattgct gataatactt atttaggcat agtagatggt aaggcagctg
    79741 acttgtatcg tattggtgaa cttcttcacg aagaactggg agaacaagtg gcagtaggct
    79801 cttcaccaca aatgcttgcc caattatcga gaaaattcat tgagaatatg gatgcaagat
    79861 ttaaaattat caaacagaat agttcattga atgctgacat ttatgagcta gatgagctgg
    79921 gactggtacc taattttttg ttcgttgatg aactcgcttc tattcgtgat agttgtggct
    79981 ctacgaaaca aggtagagaa acatggaatg aaattctaca aaatttaggc ttgattgcac
    80041 gcaaaggacg gcaggcaggt tgccacctag tcctatctac tcaagaccct aatgccgaaa
    80101 acatccccgt tgaattgagg aatcaaattt cagcagtttt ataccttggt gccccagggg
    80161 ctgatagatt gaagatggct tttagcatgt gcgaattaga aaacgttcct acagtttcag
    80221 atagaaaagg ggaggctctc ttttatgctg atgggcttaa tacagtagag ccagtgctaa
    80281 caattgtgcc atttgttgat ttaaaaacta agaaagagtt tttaaatgta gttagaaaca
    80341 tactgccaac taatgacggt cttcctcccc tagcgaaata gggcgattgc cggaggcaga
    80401 atcgagcgga gcgctctctt ttcgctaggg gagggagagc caaccgactg ctttgcagga
    80461 ggtcagaaaa aacatcccca gtactaatag ggggtgcaca catacatgaa atgtactcta
    80521 aacccttgat acgtgagggg ttacggctta ctagttgcac caaggggtcg caaactcttg
    80581 ggagagtagg gatgagaggt gtttccatgt atgatacgga attaaaggag aaaaatatgg
    80641 tttacaatat gacagataca aacattaaaa ttggattgct tgaccttgag atgtcacagt
    80701 ttgatattcc taagaaagta gtgattttat cagttagggt gaacaataaa tacaaagaag
    80761 tcaacggcga aaacgtgaag acagatgaag taagtaaaat tacttctgct gttcttgacg
    80821 ctgacaaagt gaaagttcta actgaaatgg gaatttctac tgatgactta aaagctatta
    80881 acctagaaat tatcggaaat ctagataaga ttgcaaaact tgctcaaaat gatgctttgt
    80941 tgaatgttcc tgttgaattg attcggccta aagttcgact agcttggaac atggctagga
    81001 ataattggtc tggtgtaaaa attgtgtgtg aggatattaa agttctagga gaataatcct
    81061 atgaatacag ttagaataga ttattttgct gtgacagtta agaatatttc gccagaaaaa
    81121 gttctgacag acatcctttt gattcctctt gaaaacttta ccttgaatga ttggggcata
    81181 aataagtata agcaacatta ttcttgttca gaaattaagg tgtattttaa tgctgatagg
    81241 ctttctatgg gtgtatttat tgaattaaaa gggcaaggtt gtcgtcaata tgaagaattt
    81301 ttaaacagta acgaaaataa ttggattgct ttgataagtc gtttatatca tcataatgcc
    81361 aactttacta gactagatat tgcccacgac atatttgacg gtagcttaga tgttcaacga
    81421 atatatgact attgcaagaa aggattatgt atctcgaaag caaaacactt tgaatatcat
    81481 gagaaatcag ttctagattc tggtgagcgt gtaggagaaa cagttgcaat aggttcacga
    81541 ggtaatcagc agtggtgcat atataataaa cgcatggagc aacttggtaa gcaagagctt
    81601 gtagaagttt catcttggac tcgcgcagag cttagatgtt ggcaggagaa ggctaatata
    81661 attgcagagc agttatattt aaaacgtccc ttatcgtcaa tatattttga ggctatcaac
    81721 ggtcattatc gctttattcg tccaaatgct atagatagta atcgttggag aagaaaaaag
    81781 gttgagtggt gggtaaacta tctagggaca gaaaatcaga cagttctgag tattacaagg
    81841 gctaagacga cgttacggga gtctgaagtt tggactgaga aacaggttgc aaaaacatta
    81901 gcaaaagtct atattgcaaa gtatcaggcc tatgatgttc aaaaggcgga agattatatt
    81961 caaaacttgc tcaaagaggg actttcaaag ctaactgata atgatgaaaa ggatatagaa
    82021 caatatataa gagaacagaa tagttcccaa ctttggggaa ccgatggaag gagtgactga
    82081 catatggcag atactatatc aaataaggat ttgatttcca tgggctatag gccatcaaca
    82141 gctaatgcta ttatccatca ggcgagagaa ttacttgtat cacgaggcta tacattttat
    82201 aatcgcaaac gtttgatggt tgttccaaaa agtgttgtga aagagttgtt gggaatggaa
    82261 ttgtgaaatg gcttatatcg agtataaaca gcgtggtaag aaaagacttt ggtccttctc
    82321 tatacgagaa aggagcaaga gcctactcca taaaagcgga tttaaaacaa aacgagaagc
    82381 taaaatagaa gcggagaaag ttcttcataa gttaaatact gggagtgtct taagctctag
    82441 tatgacttta tcggagcttt ataatgaatg gcttgattta aaaattttac ctagcaatag
    82501 aagtgtagtt actaaaaaaa aatatctcat gagaaagaag gtcatcgaaa ggttatttgg
    82561 aaataagcct gtatcacaaa ttaagcctag cgagtatcaa aaaattatga atgagtatgg
    82621 agagactgta tcgagaaatt tcttaggaag attgaattct tgtattcggg caagtataca
    82681 gatggctatt gctgataagg tgataataga ggattttact gcttatgttg agttgttctc
    82741 ttctaagagt ggacaaaagg ttgaggaaaa gtatttgcat gctgagtcag attatcaaaa
    82801 agttttagta tatttgaaaa ataagtttga ttatcaaaaa tctattgtac cgtatgtaat
    82861 ttatttcctt tttaaaactg gcatgcgttt ctccgaattg attgctctaa cttgggatga
    82921 agttgacgag cttaatgagc aacttaaaac ttatcgtcgt tacaacaccg ctattcataa
    82981 gtttactcca cctaaaaaca atacttcaat aagattagtt cctattactt cagatatgct
    83041 ttccttgtta aaaacattga aaatattgca attaaaaaca aataaagaat taaatattga
    83101 taatgaaaat aatttgattt ttcagcattt tggctatgtc tacgatgttc cagatattgc
    83161 aacagtcaat aaagctataa aagtaatgtt gaaggaattg caaatttttc cattgattac
    83221 taccaaagga gctagacaca cttatggtag ttatttatgg cataataata ttgatttggg
    83281 agttatagcg aaaattttag gacataaaga tatctccatg ttgattgacg tttatgggca
    83341 taccttggag gaaaaaatta gtgaagagtt tatggctgtg aaaagtttgt tgtaaaaaca
    83401 cggcaaattg cacgacaatt tttaaaagga aggcaaaaaa agccctatat tataaggctt
    83461 ttcctgttgt atttatatgc cccctacagg gatcgaacct gtgacccacg gattaagagt
    83521 ccgctgctct gccagctgag ctaaggaggc aaagaaaaaa gctgtattgg tgccgaaact
    83581 tcacggtttg tattgaaccc gcgcaattaa gcaggtgggc aactcgctct aactgaagct
    83641 gtttccgtgt gagacggcct acatgctgtt agaagacttt tgtttcccta ataatacaaa
    83701 aaatagtcgg tcaacactta agtgtgaagt cgtacaccac agcgtttcta tacttatatg
    83761 ataccacttt ttcaaaaaaa atcaagagga aagtgcaatt ttttgaaaag gatttcagat
    83821 ttttcgcaaa cggtcgatag cttcctttct ttgagccaaa gcccgttcat atttaccagt
    83881 atcttctgct tggaaatagt gatgattacg aattttttct ggcagatagt cttgcttgac
    83941 ccaatttcca ggatagttgt gtggatagag atagtcttgg gcattcccca gttccttgct
    84001 tccactgtag tgcccatcac gtaggtgtcg cggaataggc aagtgccctg atgttttgag
    84061 gtcagcaagt gccttatcca tagctacata ggctgagttg gattttggag aaagggccaa
    84121 atcaatcacg acattggcaa tgagaatgcg ggcttctggg aacccaatct tctgggcagc
    84181 atccagagca gtcacggtat gaatctgggc ctcaggattg gctaagccga tatcttcata
    84241 ggcgataaca gtcaagcgac gagcgagact aggcagatcc ccagcctcaa tcaagcgggc
    84301 agtatagtgg agactggcat ccacatctga gccacgaata gacttttgca gggcagatag
    84361 aacatcatag tgaccgtctc catccttgtc catagtgatg tagcttctct gaagactatt
    84421 ttccatgatg tctaaggtga tgtggcgaat gccctcgtca ttctcagggg tagagagaac
    84481 agctaagtcc agtgagttaa aggcagagcg aaggtctccg tttgtagagg ttgcgatgaa
    84541 atccagcgca tcctcatcta gttctattgg aaaatcaaaa ccacgttcag ggttacttag
    84601 agctatctgc agggcctctt tgacgtcttg gttagacaga ggttccaact cgaaaatttg
    84661 aactcggcta cgaatggcag gagtgacaga gaagaaagga ttttcagtcg ttgctccaat
    84721 catgatgacc agtccacttt ccaagagagg caagaggaag tcttgcttgg tcttatctag
    84781 tcgatgaatt tcgtctagca ataggacgag accaccagaa aatttagctt cttccgagat
    84841 ttcttgcagt cgctttttac tatcaactgt cgcattgaaa gttcgaaagg catacttggt
    84901 cgttccagcg atggcagagg caatactggt tttgccgatt ccaggagggc catatagaat
    84961 catggaggac aggcggttgg cttccaccat gcggcggatg atttttccag gtccgaccag
    85021 atgctcctga ccgatgacct ggtcgatggt tttagggcgc atgcgaagcg cgagattgtc
    85081 tggcatagca gtcctttcta gcgtggattt gagtagaatg actcatctac tatatcttac
    85141 tatgtccatt ctagcctatt agttgtgata tttcaatggt tttgtaataa tcagggagtg
    85201 agatagaatc ttttttacta tttaatttaa tgaggagagg tatcagcatg agagatttga
    85261 ttttccttaa agctcaaaat aggatagttg agcctaaaga agtggaagtg ctgacttgag
    85321 aaataatatt gctgaatgtc attgtatgaa ctataatgaa aagagaggtg ataagcaatg
    85381 gaaatgatgg accaagtcag gaaattttga tttttacaaa acaaattcgt cattggattc
    85441 ttagtgatca agttatttca gggaaaagaa aacttttctt ccgggaagat actccaaaag
    85501 agattttaga tttgtacgaa aacattaaat ctaaacttga ttttgcttac caataagttt
    85561 attctaataa tgggttgaaa aaatatgaaa aatagaataa ttgatgtttt tgaagtagta
    85621 aatcgcatcc ttgtaataac tgttgaaaat cctgatttcg aagacttgag agttaatcat
    85681 actcaatgaa aatcaaagag caaactagga aactagccgc aggctgtact tgagtacggt
    85741 aaggcgacgt tgacgtggtt tgaatttgat tttcgaagag tatcagtttg taaaaattgg
    85801 agataaaaaa tatagagtac gcagtgttcc tatgattcat tcaactccgc ctcaatctgt
    85861 cttagatcga gatactttta caattgacta tacagatgat gaattgctcg ataaagaagc
    85921 agtttttact actcattaga aaaataggtc tgaataaatt ggcttccctt agaaccacat
    85981 ctaggggctt ttatgtataa tagaataata agtatcactc ctttacaata cgtgtaacac
    86041 gtgttataat ggtgttgtca ggaggtaaaa gcgctatgcc tatgacacaa aaagagatgg
    86101 ttaaactcct tacggcccat ggttggataa agactagagg cggtaaaggc tcccatatta
    86161 aaatggagaa gcaaggggaa agacttatca caatccctca tggtgagctg aataagtaca
    86221 ctgaaagagg gataagaaag caagctgggt tgtaaaattc agcccctgtt ttttgatatg
    86281 gaggtagtga aatgttagtt acgtatccag ctttatttta ttatgacgac acagacggaa
    86341 cagaagcgac ttattttgtc catttcccag attttgaata ctcagctaca caaggagagg
    86401 ggatttctga ggctttggct atggggtcgg agtggctagg gataactgtt gcagatttga
    86461 tagagagtga tggagagtta cctcagccat cagatatcaa tagcttgtct ttaattgata
    86521 atgatccttt taaagatgat gaagatttcg tgtcaaccta tgaccttgat aaatctttta
    86581 tttctatggt atctgttgat gtatcagaat acctaggaag tcaggaaccc ataaaaaaga
    86641 ctttgaccat accaaaatgg gcagataagt tgggacgaga aatgggactt aacttttctc
    86701 agactttgac agacgctatt gcagataaga aagttcaagc ctaagattaa gcgtggtatc
    86761 gtggtaatag tcaataaaaa agcacgtcca cttgtgctag ttactcgcct attgatttta
    86821 tagatttatt agccctttcg cgggcttttt atattgattt ttatgagaaa tgaagaaatc
    86881 agacttttaa gaaaatcact gataccaagg gtttaaatga ggtaatatgg tataattagg
    86941 acataaaata attttgtggt aagatggtag tatctatttt agcatatttc cgagcaatcg
    87001 gggcgattaa agagtcgcat agaaagagga caaaatggca acatacggat ttttagatgt
    87061 tttagaggaa gagttggaca agaactttcc ctttgacttt gagattagct gggacaaacg
    87121 caaccatgcg gtagaagtga gttttctatt ggaagcgcaa aatgctgcag gtgtggagat
    87181 ggtggatgaa gacggagagg ttttatcaga tgacattctt tttgaggaag cggtcctttt
    87241 ctacaatcct gccaagtcaa cagtcaatga ggaagactat ttgactgtta ttccttacct
    87301 gcctaaaaaa ggattttccc gtgaattttt agcttatttt gcgcttttcc ttaaagatac
    87361 tgctgaggtt gggctagatg tcctcatgga ctttttggaa gacccagaag cagaggaatt
    87421 tgtcatggaa tggaaccaag aagtctttga agaaggaaaa gttggcttgg aagagggaga
    87481 attttatcct tatccaagat actaggagtt ggttggaggt gttatgaaga aaattgggac
    87541 ttatttggtt tatgtgctag cttttgtctt tattatgcta gcttttgctt gtggaacaat
    87601 cgcatttgca gagttggggt attccgcagt tttagccttt acttttggtt atgccttcgc
    87661 tcttctaagc atgtatttaa tctttattct tcatgagctg ggtcatgctt tttgtggcta
    87721 cttgacaggc tatcggctgg tggcttttgg attaggacat tttattttga ccaaaaagtc
    87781 aggcaggttt catcttagta gaacagccat tctgaaaaat gttggtgctc aatacattgg
    87841 tttaaaagag gatgaaagcg atcaaagaat catcctgatg ctttcaggag gcttgatggt
    87901 tcatctcggc ttgatattat tggcgatagt gtttggattt ttgacaagaa gctggtattt
    87961 tgcagggact tggatttttc ttaatttatc tttcttccta aataacattt tgccagtcga
    88021 catcaccgat ggagcaaaaa tttgggaatt actacaacac cctgaaaata ccaaatacgc
    88081 ctacctggta ttgagacatt ctgctcaaac tttgctggct cctcaagaat atgatttgaa
    88141 agactttgtt caagcagttc ctgaggatgc acgagggagt tttgcggatg gtgtattagg
    88201 tatgcaaggt gaagtttcca ttttggaagg gaaggaagaa gtagctaaac agcagtttca
    88261 agcattactt gagaggacaa ctactccgat gatgcagaca gttgcccaac tatccctgct
    88321 tcatatcgcc ttgttggaag gagattttga gcaagcggag caatatgcta gtattcgaca
    88381 ggtcaaatcg tttctgtctc tcaaactgtc caatcttcaa gtgatacaag cttggtatca
    88441 atttaaggta aagaaagatg tagatcgaac tcgtaaggct ctcaagattg ctagacagaa
    88501 aatggatagt agtcgaatgt tgagggacga gaaacgctat tatgaaaact tgttagcaga
    88561 gctggaaaag gaaatgttag aaggaactga agatggaact agaaatttct gatttcccag
    88621 gctgtaagat tgctttgttt tgtggggata agctcttgac tatcttacgt gatgataagg
    88681 caagtattcc ctgggccaat atgtgggaac tgccaggtgg tggacgtgaa ggtgatgaaa
    88741 gtccttttga gtgtgtagcg cgtgaagttt atgaagaact gggcattcat ctgactgaag
    88801 attgtctgct ttggagtaag gtttacccaa gtatgctttt tgcagacaaa cagtccgttt
    88861 ttctagttgg tcagttgaca cagaaccagt ttgacagtat cgtatttgga gatgaaggac
    88921 agggctatca gctgatgaat gttgaggaat ttcttagttc cagtcaagtt gtccctcagt
    88981 tgcaagagag attaaaagat tatttaaaag taagtgatta gaaaggatgg ttcagttaaa
    89041 tttctaaact gaacccgccc taaacactgt gccaaaaaga taaacttctc ttagacacaa
    89101 gcgtcttcag agaatttcct attttggttt tgtgttttac gggcttggta tcttaataat
    89161 ggaaacatgg caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa
    89221 tctcttgatt gagctgggag cgcaaggagt cgcaatcgaa gacagtatgg actatgtggg
    89281 aaatgttgac cgctttggcg agattttccc agaggtggag cagcaagaag aaatcgttgt
    89341 gacagcctac taccctgaca cggtggatgt gacagtggtt gaggcggact tgcaggctcg
    89401 catatcagaa ttgacggatt ttatggattt gggagagtta aaaataggta cgactgcctt
    89461 ggctgaggaa gactgggcag acaactggaa gaaatactat gagccagctc gtatcactca
    89521 tgatttgacc atcgttccct cttggacaga ctatgaggcg actgcgggag aaaaaattat
    89581 caagctggat cctggcatgg cttttggtac tggaacccac ccaactacca agatgagcct
    89641 ttttgccttg gaacaggttc ttcgtggtgg cgaaacggtg ctagatgtgg ggactggttc
    89701 aggggttctc tctattgcca gctcgcttct gggggctaaa gaaattttcg cctatgacct
    89761 ggatgatgtg gcggttcgtg tggctcagga aaatattgag ctcaaccctg gcatggaaaa
    89821 tatccatgta gcggctggag atttgcttaa gggtgtggaa attgaggcag atgtgattgt
    89881 ggctaatatc ttggcggata tcctcattca tctaatagac gatgcctatc gtttggttaa
    89941 ggacgaaggc tacctgatca tgagtggcat tatcaaggac aagtgggaca tggtgcgcca
    90001 gtcggctgag tcagctggat ttttcctcga aactcacatg gttcaagggg aatggaatac
    90061 ctgtgtcttt aagaaaacca aggatatctc tggtgtgatt ggaggctagc atgcagcagt
    90121 attttgtaaa aggcagtgct atctctcctg tcactatcga ggacaaggaa accagtaagc
    90181 atatgtttca ggttatgcgc ttgaaagaag aggatgaggt taccttagtc tttgatgatg
    90241 gcatcaagcg cttggcgcgc gtgctggata tggaaaatcg tcagtttgag ttggtccaag
    90301 aattaaatga caatgtggaa ctaccagtcc aagtgaccat cgcatctgga tttcccaagg
    90361 gagacaagct ggagttcatc gctcaaaaag taaccgaact gggtgctagc caaatctggg
    90421 cctttcctgc agactggtca gttgccaagt gggatggcaa gaaattgggt aaaaaagttg
    90481 aaaaactaga aaaaattgcc cttggagcag ccgagcaaag caagcgtaat attgtaccaa
    90541 gtattaaact tttcgagaaa aaagcagatt ttctagctca gctggaccag tttgacttta
    90601 tcatagtagc ctatgaagaa tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag
    90661 tcactggtct tgaaacagga gccaaactgc tctttatctt tggtccagaa ggcggtctct
    90721 cacctgcaga aatcgaaagt tttgaagcta agggagccgt tttggtaggc ctaggtcctc
    90781 gtattttgcg agcagaaaca gcaccgcttt acgccttatc agcccttagt gttttagtag
    90841 aattagagaa ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag
    90901 gaactctggg acttaacagc cctataccaa gaccgtgagg atttcttgcg tgcaatcgag
    90961 aaagctcgtg aagacatcaa ccagtttagc cgtgattaca agggcaatct tcacactttt
    91021 gaggatttcg agaaggcctt tgcggaatta gaacagatct acattcagat gagccatatt
    91081 ggcaactatg cttttatgcc tcagacgacg gactatagca atgacgaatt tgccaatatt
    91141 gcccaagctg ggatggaatt tgaaacagat gccagcgtag ccttgacctt ctttgacgat
    91201 gccttggtgg tagcagacga ggaagtcttg gaccgtttgg gtgaattgcc tcacttgacg
    91261 gcagctattc gtcaggccaa aatcaaaaaa gcccactatc taggggctga tgtggagaag
    91321 gccttgacca atctcggtga agttttctat agtccgcagg acatttatac taagatgcga
    91381 gctggggatt ttgaaatggc tgactttgaa gcccatggca agacctataa aaacagcttt
    91441 gtcacctatg agaatttcta ccaaaatcac gaggatgctg aggttcgtga gaaatccttc
    91501 cgttccttct cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct
    91561 caggtcaagt ctgaaaaact cttggctgat atgaagggat acgactctgt atttgattat
    91621 cttctagctg aacaagaagt ggaccgtgtc atgtttgacc gccagattga cctcatcatg
    91681 aaggactttg caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt
    91741 gaaaagatga cctttgcaga ctggaaattg gacttggaca gcgccctgaa tcctgaagtg
    91801 actattgacg atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat
    91861 tgtcaggaag ttgctcgtta ccaagaagag cgctgggtgg actttgctgc taacagtggc
    91921 aaggattccg gtggttatgc ggcggaccca tatcgcgtac acccttatgt actcatgagc
    91981 tggacaggcc gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa
    92041 ttcatctttt cagacaatca ccaaagctat ttcaacgccc acatgtcgac ctattatgtt
    92101 gaagcaccgt caaccttcaa tgaattgcta ctcagtgatt acttagagaa ccagtctaat
    92161 gacccacgtc aaaaacgctt cgctctggct catcgcttga cagacaccta cttccataac
    92221 tttatcaccc acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa
    92281 ggggagacct ttggagcaag caaactcaac agcattatga aggaagtttt gacagatttt
    92341 tggggcgatg ccattgaaat cgacgacgat gcagctttga cttggatgcg ccaagctcac
    92401 tactatatgg gcttgtatag ttacacttac tctgctggtc ttgtcatctc gacagctggt
    92461 taccttcatc ttaaaaactc agaaactgga gctgaagact ggctcaatct cctcaaatca
    92521 ggtggtagca agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac
    92581 aaaccactcc gtgataccat ccaattcttg tctgacacag ttgaccagat tatcgcctac
    92641 agtgctgagt tgggggagta aggggataga gaggttgtta tagaatgggt atggacagtt
    92701 atttgcttaa aagtccatgt ttaaatcccc cattcctttt cgaggaattc tttgccctgc
    92761 tctgtaatgg taagtgaacg tggcgtttgt gcagggatga ccaattgttt ttcactgaaa
    92821 aattctaaaa gagcattgcc gagagctccg ccgacatgat ttttacgttc gctccaatcc
    92881 aaacatttag ggcaaaattt ccgttttttc tttttcaatt catccgtatc aatgtgtaat
    92941 tttttttgga aataagactt cccagcttca gtgagttgga agtctttttc tgattcgctg
    93001 aggagatttt cttgcaagag ataattggta atggaaatgc caagctcacc agcaatatgg
    93061 tcatagcagg tgcgagcgac ctttaattcc tttttgtgaa tgtgttgttt ataacttttg
    93121 acatcacgct gaggagagta gaagctgaga aattccagca tttctgcaat ggcagcactt
    93181 tttaaagaat aataatgaaa acgtccatag gtttgtagag tggttacctg cgcttcggta
    93241 agtaatttga ggtgatagct gacagtatgt tgcttgattt tggtaaagct ggcaatttcg
    93301 ttaaccgtgt gagcttttcc atccattaga atatctaaaa tgtgcatccg agaagggtct
    93361 gataagagtt ttgtgatgat gaaaatatct ggttccataa aaattttctc caaatttcat
    93421 atatcgatta ttttctatct tttattatac tataatcaaa gcacaagaaa aatagatttt
    93481 aaaaatgttt tactagagaa tattttaaat tttattgtct gagtttaaat ggaggtaaaa
    93541 ttacaaaagt aattgcagca gtatccacag ttgaaggacg ttaatcttaa gattacattc
    93601 tcaacatttt ttcaaaagtg agatgaatta acaaagtatc caacatgctc tatttgttgg
    93661 ggaaagaaag agtgaagaag ttaagtgtta aaaatctaaa aaagcaatat ggttcttatg
    93721 aagctgtcaa gggaattagc tttgaattgg gagaaggaga gtgtttgggt atcttgggaa
    93781 tcaacggtgc tggcaaaaca tccactttaa agatgattta ctcactgacc tcaatttcct
    93841 caggagagat tgatatcttg gggcaaaata ttgaaaccaa tcgtcgtaag agtaaatctt
    93901 gtctgggtat tgtagggcag gaggatatgc tggatatgac cttgaatgtt tatgataatc
    93961 tttttgctca cggtctatgt tatggtatct catctgtcga acttaggaag agaatcgatt
    94021 ttctcttgga ttttgttcaa ctgagcaaac atgcgcaaaa gatggtgaat cagctgtcgg
    94081 gtgggatgcg acgacgtctt gtcttggcaa gagctctcat caatcggcct gatattatca
    94141 ttttagatga accgacagtt ggtttagaca ttcaatcccg caatattatt tgggataaac
    94201 ttttggagtt gaaggaatta ggtgtttcca tcattattac ttcccactat atgaatgaga
    94261 ttgaatattt gacagatagg gtgctgatgc tccaccaagg gactatcaaa gaagaaggaa
    94321 ctgtagaaga cttgctcatc aagtatgatg ctgacaacct tgagacgctt tttttggggt
    94381 tgactgggac agagaaggag gacttgcatg tttaagttat catttcactc catcggacac
    94441 gttgtggtac gcaactacat gagttttaga aatttattta aaatttcaat tgtccccaac
    94501 cttatcgacc ccctctttta tcttttagca atgggatttg gagttggtgc ttatttgacg
    94561 catgttaacg gtatgctcta ccgtgatttt gtcattacag gtttaattgc tgcgactgcc
    94621 atgtctgcgg ccactgcaga aacaacggtc aatgccttta tccagtataa gattgaaaag
    94681 acttatgatg ctatgttgat gacgccaatc aatacgaatg atattgtggt gggtcaggct
    94741 atctgggcag gtattcgtgc agtgattttc ggtgggattt tttggctgat ttccatgctt
    94801 atcacagcgc attttcatgt cttgatgtta ttgattcctc tgattttatt tatagtgggt
    94861 tatttgtttg gtgttttggg actaatcttt acctacttgg cgccgtctag ggaatttctg
    94921 aattactaca atgttttggt aatccgtcct ctttatatgt tttcagatac cttctttccc
    94981 attacttcct tgcccccaat acttggtgat ttgacttggt tttctccctt gtatcacgct
    95041 acccgcatga ttcgtattat atggggaggt gaggtgacag gtttgtatct tcatcttatc
    95101 tggttgatag ccttggcgct cttgataacg gtgcttccta tgtatatgtt acatcgtaga
    95161 tattatagat agtctaatta tttgtgatgt tgtagtcata ttctaattta tactaaacgt
    95221 tattcgaatt tggacaggta tgggaactgt gcctatcaat attgtaattt atggaagttg
    95281 aaatagtacg gttcagtttt taaaatattg ttagaaattg ttttaaattc cctaatcaat
    95341 ttgtatagtt tttatttcac tttattataa ctagtttttc gcaatttttg agacaggaat
    95401 gtgatttttg tgataaggct ctatcattac aagttcattt gtggtcatat ggaataaatc
    95461 atttatatat cttttcagaa atatgctgtt tctctgaata taaatgtctt tttcttttta
    95521 acttaatttt acaaattggt aaagtcattt aagactgcct ttttgaacag ttgctagaaa
    95581 tgtagaggta ttgaccatgt atcaagattt actaagaaaa atagcagaag aaaaaccaaa
    95641 ttataaccag gaagaaatcc agtggttgtt tgatcatttg ggaaatcctt ctccagaaat
    95701 tcgtgatgac cttgttttta caagctttgc taaggagatt caggaagagc tatttacaca
    95761 ggaacaattt catttcattg ctgaggtggt cttagctgat ggagggttag ataaagagat
    95821 tgataaggta ggcttgtcaa cacttgaacg ttcttttagg gcacttattt atgcaaatct
    95881 cttgtctgca gatgctaacc agcaatcggt tttttatcag ggattacaat cagaaattcg
    95941 taatgtcctt ttaaatcaag gcttgcacta tctttcaaaa gaaaaggata caacaggttt
    96001 ctcaagtcag tatggttggg ttcattcttt tgcacatgga gccgatttac tgacagaggt
    96061 ggtctgtcat ccagactttc ctataaacag aattcatgaa gtatttgata tacttggcca
    96121 actatttaaa agaatgtcga ttctctttac agatgatgag gattggcgtt tagcaagagt
    96181 gatctatgaa cctattttac aagggaagtt ggagcaagaa caagtagctt cttggataaa
    96241 aactgttgat tttccgatag aagaaaggga ggatttttac aaattttcca actttagatc
    96301 ctgtctggtg gaagtctatg tccaacttga ccagagaaat agtttacaag atgacctgaa
    96361 agaagctatc cagtcttttc aatactaggg attagctaat agttctatga ttttcaaaaa
    96421 atttccaatc aattttcttg aaaccctttc ctacttttga tagactagta tctagttttt
    96481 gaaaaaagga gaaagaaaat gaaaaaattt gttgctgagt taatcggtac gttcatgctt
    96541 gtgttcgttg ggacaggagc tgttgttttt ggaaatggtc ttgatggcct tggtcacctt
    96601 ggaatcgcct ttgcctttgg tttggcaatc gtggtggcag cctactcaat cggaactgtt
    96661 tcaggtgctc acttgaaccc ggctgtttcg attgctatgt ttgcaaacaa acgtttgtca
    96721 tcttcagaac ttgtaaacta catccttggt caggttgttg gagctttcat cgcttctggc
    96781 gcagtcttct tcctcttggg taattcaggt atgtcaactg ctagtcttgg tgaaaatgcc
    96841 ttggcaaacg gtgtcactgt ctttggtggt ttcttgtttg aagtcatcgc aactttcttg
    96901 tttgtcttga ttatcatgac tgtgacttca gaaagtaagg gcaatggcgc gattgctggt
    96961 ttggtaatcg gtttgtcatt gatggcgatg attcttgtcg gactgaagat tactggactt
    97021 tcagtaaacc cagctcgtag cttggcacca gctgtcttgg taggcggcgc agcccttcaa
    97081 caagtttgga ttttcatcct tgcaccaatc gctggtggag ttcttgcagc ccttgttgca
    97141 aaaaatttcc ttggaacaga agaataattg aaactcaaaa agccttgctc ctcatcttga
    97201 ggaacagggc tttttcgtat gatactcttc gaaaatctct tcaaaccacg tcagcttcac
    97261 cttgccgtag gtatggttac tgacttcgtc agtttcatct
//
LOCUS       DBERYS010000003        81939 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.003, whole genome shotgun sequence.
ACCESSION   DBERYS010000003 DBERYS010000000
VERSION     DBERYS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 81939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.003"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            643..1095
                     /locus_tag="ACL8LA_000218"
     CDS             643..1095
                     /locus_tag="ACL8LA_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1812047.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKAGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1114..2451
                     /locus_tag="ACL8LA_000219"
     CDS             1114..2451
                     /locus_tag="ACL8LA_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812048.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2530..3414
                     /locus_tag="ACL8LA_000220"
     CDS             2530..3414
                     /locus_tag="ACL8LA_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812049.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWIINGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3414..4247
                     /locus_tag="ACL8LA_000221"
     CDS             3414..4247
                     /locus_tag="ACL8LA_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812050.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4265..6358
                     /gene="nanB"
                     /locus_tag="ACL8LA_000222"
     CDS             4265..6358
                     /gene="nanB"
                     /locus_tag="ACL8LA_000222"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HGS1812051.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6370..7473
                     /locus_tag="ACL8LA_000223"
     CDS             6370..7473
                     /locus_tag="ACL8LA_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1812052.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7647..8345
                     /locus_tag="ACL8LA_000224"
     CDS             7647..8345
                     /locus_tag="ACL8LA_000224"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS1812053.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8364..9881
                     /locus_tag="ACL8LA_000225"
     CDS             8364..9881
                     /locus_tag="ACL8LA_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS1812054.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            10006..11334
                     /locus_tag="ACL8LA_000226"
     CDS             10006..11334
                     /locus_tag="ACL8LA_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812055.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11586..12452
                     /locus_tag="ACL8LA_000227"
     CDS             11586..12452
                     /locus_tag="ACL8LA_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812056.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            12468..13307
                     /locus_tag="ACL8LA_000228"
     CDS             12468..13307
                     /locus_tag="ACL8LA_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812057.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13579..14031
                     /locus_tag="ACL8LA_000229"
     CDS             13579..14031
                     /locus_tag="ACL8LA_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1812058.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14128..14472
                     /locus_tag="ACL8LA_000230"
     CDS             14128..14472
                     /locus_tag="ACL8LA_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812059.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14482..14676
                     /locus_tag="ACL8LA_000231"
     CDS             14482..14676
                     /locus_tag="ACL8LA_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812060.1"
                     /translation="MKALCIFLLYMLAWTVLAMIIRGGLDGFDEHTWRTILIASLSGI
                     YIDYMSIDKSRKKSKRKKKK"
     gene            14759..15403
                     /locus_tag="ACL8LA_000232"
     CDS             14759..15403
                     /locus_tag="ACL8LA_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS1812061.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15572..16489
                     /locus_tag="ACL8LA_000233"
     CDS             15572..16489
                     /locus_tag="ACL8LA_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGS1812062.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            16507..17391
                     /locus_tag="ACL8LA_000234"
     CDS             16507..17391
                     /locus_tag="ACL8LA_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1812063.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17407..18258)
                     /locus_tag="ACL8LA_000235"
     CDS             complement(17407..18258)
                     /locus_tag="ACL8LA_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS1812064.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18458..20515
                     /gene="pbp2b"
                     /locus_tag="ACL8LA_000236"
     CDS             18458..20515
                     /gene="pbp2b"
                     /locus_tag="ACL8LA_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGS1812065.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20526..21122
                     /gene="recR"
                     /locus_tag="ACL8LA_000237"
     CDS             20526..21122
                     /gene="recR"
                     /locus_tag="ACL8LA_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGS1812066.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21298..22341
                     /locus_tag="ACL8LA_000238"
     CDS             21298..22341
                     /locus_tag="ACL8LA_000238"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGS1812067.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22425..23798
                     /gene="murF"
                     /locus_tag="ACL8LA_000239"
     CDS             22425..23798
                     /gene="murF"
                     /locus_tag="ACL8LA_000239"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGS1812068.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            23785..24396
                     /locus_tag="ACL8LA_000240"
     CDS             23785..24396
                     /locus_tag="ACL8LA_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGS1812069.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24487..25191
                     /locus_tag="ACL8LA_000241"
     CDS             24487..25191
                     /locus_tag="ACL8LA_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGS1812070.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25410..26783
                     /gene="ftsA"
                     /locus_tag="ACL8LA_000242"
     CDS             25410..26783
                     /gene="ftsA"
                     /locus_tag="ACL8LA_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGS1812071.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26800..28059
                     /gene="ftsZ"
                     /locus_tag="ACL8LA_000243"
     CDS             26800..28059
                     /gene="ftsZ"
                     /locus_tag="ACL8LA_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGS1812072.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28064..28735
                     /locus_tag="ACL8LA_000244"
     CDS             28064..28735
                     /locus_tag="ACL8LA_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGS1812073.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28745..29284
                     /locus_tag="ACL8LA_000245"
     CDS             28745..29284
                     /locus_tag="ACL8LA_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGS1812074.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29284..29547
                     /locus_tag="ACL8LA_000246"
     CDS             29284..29547
                     /locus_tag="ACL8LA_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGS1812075.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29544..30329
                     /locus_tag="ACL8LA_000247"
     CDS             29544..30329
                     /locus_tag="ACL8LA_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGS1812076.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30338..31126
                     /gene="divIVA"
                     /locus_tag="ACL8LA_000248"
     CDS             30338..31126
                     /gene="divIVA"
                     /locus_tag="ACL8LA_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGS1812077.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31474..34266
                     /gene="ileS"
                     /locus_tag="ACL8LA_000249"
     CDS             31474..34266
                     /gene="ileS"
                     /locus_tag="ACL8LA_000249"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGS1812078.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            34669..35049
                     /locus_tag="ACL8LA_000250"
     CDS             34669..35049
                     /locus_tag="ACL8LA_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812079.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            35243..35533
                     /locus_tag="ACL8LA_000251"
     CDS             35243..35533
                     /locus_tag="ACL8LA_000251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812080.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            35584..36276
                     /locus_tag="ACL8LA_000252"
     CDS             35584..36276
                     /locus_tag="ACL8LA_000252"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGS1812081.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36329..38740
                     /locus_tag="ACL8LA_000253"
     CDS             36329..38740
                     /locus_tag="ACL8LA_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS1812082.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAVRASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            38990..39691
                     /locus_tag="ACL8LA_000254"
     CDS             38990..39691
                     /locus_tag="ACL8LA_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812083.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39693..42401
                     /locus_tag="ACL8LA_000255"
     CDS             39693..42401
                     /locus_tag="ACL8LA_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS1812084.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGIVVGETQIQFYWTYSLLALVLSWLASVLPVYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSETPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42497..42988)
                     /gene="tpx"
                     /locus_tag="ACL8LA_000256"
     CDS             complement(42497..42988)
                     /gene="tpx"
                     /locus_tag="ACL8LA_000256"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGS1812085.1"
                     /translation="MVTFLGNPVSFTGKQLQGGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43111..44040)
                     /gene="psaA"
                     /locus_tag="ACL8LA_000257"
     CDS             complement(43111..44040)
                     /gene="psaA"
                     /locus_tag="ACL8LA_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGS1812086.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(44072..44920)
                     /locus_tag="ACL8LA_000258"
     CDS             complement(44072..44920)
                     /locus_tag="ACL8LA_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGS1812087.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44917..45639)
                     /locus_tag="ACL8LA_000259"
     CDS             complement(44917..45639)
                     /locus_tag="ACL8LA_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS1812088.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            45930..47822
                     /gene="pepO"
                     /locus_tag="ACL8LA_000260"
     CDS             45930..47822
                     /gene="pepO"
                     /locus_tag="ACL8LA_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGS1812089.1"
                     /translation="MTRYQDDFYDAINGKWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(47945..48574)
                     /locus_tag="ACL8LA_000261"
     CDS             complement(47945..48574)
                     /locus_tag="ACL8LA_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS1812090.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(48914..49156)
                     /locus_tag="ACL8LA_000262"
     CDS             complement(48914..49156)
                     /locus_tag="ACL8LA_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812091.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            49567..51789
                     /locus_tag="ACL8LA_000263"
     CDS             49567..51789
                     /locus_tag="ACL8LA_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HGS1812092.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            51819..52262
                     /gene="dtd"
                     /locus_tag="ACL8LA_000264"
     CDS             51819..52262
                     /gene="dtd"
                     /locus_tag="ACL8LA_000264"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGS1812093.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            53053..53436
                     /locus_tag="ACL8LA_000265"
     CDS             53053..53436
                     /locus_tag="ACL8LA_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812094.1"
                     /translation="MGKENVRMRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVR
                     EYVPKKERIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQ
                     AKRIHRAFCIRLCLRAQFLLSNNDS"
     gene            53844..54143
                     /locus_tag="ACL8LA_000266"
     CDS             53844..54143
                     /locus_tag="ACL8LA_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812095.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(54238..54675)
                     /locus_tag="ACL8LA_000267"
     CDS             complement(54238..54675)
                     /locus_tag="ACL8LA_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812096.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(54806..55357)
                     /locus_tag="ACL8LA_000268"
     CDS             complement(54806..55357)
                     /locus_tag="ACL8LA_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0034258 - nicotinamide riboside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HGS1812097.1"
                     /translation="MISVTNVGISMLSTRFTGKLSKWENYFGIVNTILSGAIDYILGN
                     KAAIITYPVTFLIYTFAIKKWKASQEGRPNQMSQKQVKLAAIIISIIAFLFAFVTNYI
                     GYGGKMNLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANI
                     GKYIFYILNAIGALFVWNDEEVR"
     gene            complement(55651..56301)
                     /locus_tag="ACL8LA_000269"
     CDS             complement(55651..56301)
                     /locus_tag="ACL8LA_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGS1812098.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEILE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            56413..57255
                     /locus_tag="ACL8LA_000270"
     CDS             56413..57255
                     /locus_tag="ACL8LA_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1812099.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            57331..57768
                     /locus_tag="ACL8LA_000271"
     CDS             57331..57768
                     /locus_tag="ACL8LA_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGS1812100.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            57836..58138
                     /locus_tag="ACL8LA_000272"
     CDS             57836..58138
                     /locus_tag="ACL8LA_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812101.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(58229..59302)
                     /locus_tag="ACL8LA_000273"
     CDS             complement(58229..59302)
                     /locus_tag="ACL8LA_000273"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGS1812102.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            59458..60135
                     /locus_tag="ACL8LA_000274"
     CDS             59458..60135
                     /locus_tag="ACL8LA_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1812103.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            60128..61102
                     /locus_tag="ACL8LA_000275"
     CDS             60128..61102
                     /locus_tag="ACL8LA_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGS1812104.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(61151..63094)
                     /gene="thrS"
                     /locus_tag="ACL8LA_000276"
     CDS             complement(61151..63094)
                     /gene="thrS"
                     /locus_tag="ACL8LA_000276"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGS1812105.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(63152..63475)
                     /locus_tag="ACL8LA_000277"
     CDS             complement(63152..63475)
                     /locus_tag="ACL8LA_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS1812106.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(63906..64106)
                     /locus_tag="ACL8LA_000278"
     CDS             complement(63906..64106)
                     /locus_tag="ACL8LA_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812107.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            64344..64973
                     /locus_tag="ACL8LA_000279"
     CDS             64344..64973
                     /locus_tag="ACL8LA_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1812108.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            65114..65383
                     /gene="rpsO"
                     /locus_tag="ACL8LA_000280"
     CDS             65114..65383
                     /gene="rpsO"
                     /locus_tag="ACL8LA_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGS1812109.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            66198..66812
                     /locus_tag="ACL8LA_000281"
     CDS             66198..66812
                     /locus_tag="ACL8LA_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGS1812110.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(67104..67853)
                     /locus_tag="ACL8LA_000282"
     CDS             complement(67104..67853)
                     /locus_tag="ACL8LA_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGS1812111.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            67986..70322
                     /locus_tag="ACL8LA_000283"
     CDS             67986..70322
                     /locus_tag="ACL8LA_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS1812112.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLMETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDSIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            70424..71101
                     /locus_tag="ACL8LA_000284"
     CDS             70424..71101
                     /locus_tag="ACL8LA_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGS1812113.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            71088..71885
                     /locus_tag="ACL8LA_000285"
     CDS             71088..71885
                     /locus_tag="ACL8LA_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGS1812114.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            71902..73005
                     /locus_tag="ACL8LA_000286"
     CDS             71902..73005
                     /locus_tag="ACL8LA_000286"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1812115.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            73075..73203
                     /locus_tag="ACL8LA_000287"
     CDS             73075..73203
                     /locus_tag="ACL8LA_000287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812116.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSNHTHGKATLTWFELDFRRV"
     gene            73306..74325
                     /gene="galE"
                     /locus_tag="ACL8LA_000288"
     CDS             73306..74325
                     /gene="galE"
                     /locus_tag="ACL8LA_000288"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1812117.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            74327..75280
                     /locus_tag="ACL8LA_000289"
     CDS             74327..75280
                     /locus_tag="ACL8LA_000289"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1812118.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(75342..75548)
                     /locus_tag="ACL8LA_000290"
     CDS             complement(75342..75548)
                     /locus_tag="ACL8LA_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGS1812119.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            75600..76076
                     /locus_tag="ACL8LA_000291"
     CDS             75600..76076
                     /locus_tag="ACL8LA_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGS1812120.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            76085..76756
                     /gene="cmk"
                     /locus_tag="ACL8LA_000292"
     CDS             76085..76756
                     /gene="cmk"
                     /locus_tag="ACL8LA_000292"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGS1812121.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEDLLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            76887..77225
                     /locus_tag="ACL8LA_000293"
     CDS             76887..77225
                     /locus_tag="ACL8LA_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGS1812122.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            77228..77857
                     /locus_tag="ACL8LA_000294"
     CDS             77228..77857
                     /locus_tag="ACL8LA_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGS1812123.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            78097..79248
                     /locus_tag="ACL8LA_000295"
     CDS             78097..79248
                     /locus_tag="ACL8LA_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1812124.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            79561..80310
                     /gene="truA"
                     /locus_tag="ACL8LA_000296"
     CDS             79561..80310
                     /gene="truA"
                     /locus_tag="ACL8LA_000296"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGS1812125.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            80300..81073
                     /locus_tag="ACL8LA_000297"
     CDS             80300..81073
                     /locus_tag="ACL8LA_000297"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS1812126.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            81051..81512
                     /locus_tag="ACL8LA_000298"
     CDS             81051..81512
                     /locus_tag="ACL8LA_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1812127.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            81739..>81939
                     /locus_tag="ACL8LA_000299"
     CDS             81739..>81939
                     /locus_tag="ACL8LA_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1812128.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKGKNRYYSPYLKQEMI"
BASE COUNT        24501 a        14838 c        18277 g        24323 t
ORIGIN      
        1 taaaaaagat ttgaaactaa attccaaatt agaaaaagac ttgaaatgct aaaaaaaaaa
       61 agtatactct aattgaaaac ggtaacaaaa ctaatttaga gaatgaaata tagagtattt
      121 ctctcttaaa agtttttggt gaaacgagat gtagaaagga gatttagcca aagagtctat
      181 tagtgctaga ataatagatt agaattattt tagaaaaacg aagtgagcag cttataaatt
      241 caagtcccca aatagattca tactagtatc ttttgcaaaa aataaagggc gacttccttc
      301 atgaatatca atttcatcta taaggaaggt agctaattga actaacttat ttattctgtt
      361 tgtcgctaga aaaatcagac ctccttgtga agattgagga gatacttaat gaaaatcaaa
      421 gaagaaacta gcaagctagt agcagattgc ccaaaacacc gctttgaggt tgtagataag
      481 actgacgtat ataatccaag gtgaagcgac tgtggtttga agagattttc aaagagtata
      541 ggctagagag tagtgttttt atgtccttct agtagaaaat gctagacaga agaatgggga
      601 acttggatag gagaaataga ttgagaaagg aggttagaag agatgattat tacaaaaatt
      661 agccgtttag gaacttatgt gggagtaaat ccacattttg caacattaat agattttcta
      721 gaaaaagcag gactagaaaa tttaacagaa ggttcgattg ctatcgatgg taatcgattg
      781 tttgggaatt gctttactta tctagcagat ggtcaagcag gggctttctt tgaaacacac
      841 caaaaatatt tggatattca tttagttttg gaaaacgaag aagccatggc tgttacatcg
      901 ccggaaaatg taagcgttac ccaagaatat gatgaagaga aagatattga attatacaca
      961 gggaaagtgg aacagttggt tcagttgaga gctggcgaat gcctcatcac ttttccagaa
     1021 gatttacatc aacccaaggt tcgtataaat gatgaacctg tgaaaaaagt tgtctttaaa
     1081 gttgcgattt cttaatgtag aaagagaaga acgatgaaaa aaatgagaaa gtttttatgt
     1141 ctagctggaa ttgcgctagc ggctgttgcc ttggtagctt gttcaggaaa aaaagaagct
     1201 acaactagta ctgaaccacc aacagaatta tctggtgaga ttacaatgtg gcactccttt
     1261 actcaaggac cccgtttaga aagtattcaa aaatcagcag atgctttcat gcaaaagcat
     1321 ccaaaaacga aaatcaagat tgaaacattt tcttggaatg acttctatac taaatggact
     1381 acaggtttag caaatggaaa tgtgccagat atcagtacag ctcttcctaa ccaagtaatg
     1441 gaaatggtca actcagatgc tttggttccg ctaaatgatt ctatcaagcg tattggacaa
     1501 gataaattta acgaaactgc cttaaatgaa gcaaaaatcg gagatgatta ctactctgtt
     1561 cctctttatt cacatgcaca agtcatgtgg gttagaacag atttgttaaa agaacataat
     1621 attgaggttc ctaaaacttg ggatcaactc tatgaagctt ctaaaaaatt gaaagaagct
     1681 ggagtttatg gcttgtctgt tccgtttgga acaaatgact taatggcaac acgtttcttg
     1741 aacttctacg tacgtagtgg tggaggaagc ctcttaacaa aagatcttaa agcagacttg
     1801 acaagccaac ttgctcaaga tggtattaaa tactgggtta aattgtataa agaaatctca
     1861 cctcaagatt ctttgaactt taatgtcctt caacaagcta ccttgttcta tcaaggaaaa
     1921 acagcatttg actttaactc tggcttccat atcggaggaa ttaatgccaa cagtcctcaa
     1981 ttgattgatt cgattgatgc ttatcctatt ccaaaaatca aagagtctga taaagaccaa
     2041 ggaattgaaa cctcaaacat tccaatggtt gtttggaaaa attcaaaaca tccagaagtt
     2101 gctaaagcat tcttagaagc actttataat gaagaagact acgttaaatt ccttgattca
     2161 actccagtag gtatgttgcc aactattaag gggattagcg attctgcagc ctataaagaa
     2221 aatgaaactc gtaagaaatt taaacatgct gaagaagtaa ttactgaagc tgttaaaaaa
     2281 ggtactgcta ttggttatga aaatggacca agtgtacaag ctggtatgtt gactaaccaa
     2341 cacattattg aacaaatgtt ccaagatatc attacaaatg gaacagatcc tatgaaagca
     2401 gcaaaagaag cagaaaaaca attaaacgat ttatttgagg ctgttcagta gatgtaaaag
     2461 actagaaaat aggtgggcta gtgagctgaa aagctctagc ccaatcttgt aaaagaaggg
     2521 agaaggagaa tggttaaaga acgtaattta actcgctgga tatttgtttt gccagctatg
     2581 attatcgtag gattactctt tgtttatccg tttttctcga gtatttttta tagctttacc
     2641 aataagcatt tgattatgcc taattataaa tttgttggtt tggctaacta taaagctgtg
     2701 ctatcagatc ccaacttctt taatgcgttc tttaattcaa ttaagtggac cgttttctca
     2761 ttagttggtc aagttttagt agggtttgta ttggctttag ctcttcacag agtacgccac
     2821 ttcaagaaat tatataggac cttattgatt gttccttggg catttcctac catcgttatt
     2881 gccttctctt ggcagtggat tataaacggg gtttatggct acttacctaa tctaatcgta
     2941 aaattaggtt taatggaaca tacacctgca tttttgacag atagtacatg ggcattccta
     3001 tgtttggtgt ttatcaacat ttggtttgga gcaccaatga ttatggttaa tgtgctttca
     3061 gctttgcaaa cagtaccaga agaacaattt gaggctgcta agatagatgg tgcttcaagt
     3121 tggcaggtgt tcaagtttat cgtctttcca catattaaag tggttgtagg acttctagtt
     3181 gttttgagaa ctgtatggat ctttaataac tttgacatta tctacctcat tactggtggt
     3241 ggaccagcca atgctacaac gacgcttcca atttttgctt acaacctggg ttggggaact
     3301 aaattgttgg gtcgtgcttc agcagttaca gtattgctct ttatcttctt ggtggcgatt
     3361 tgctttatct actttgctat catcagtaag tgggaaaagg agggtagaaa ataatgaaga
     3421 agaaatccag tatttattta gatattctct cacatgtact tttagttggt gcgaccatcg
     3481 ttgcaatttt cccattggta tggattatca tatcttctgt caaagggaaa ggggaattaa
     3541 ctcagtatcc aacacgattt tggcctgaac agtttacatt agattatttc actcatgtta
     3601 tcaacgattt gcacttcatt gataacattc gaaacagttt aatcattgcc ttggctacaa
     3661 ccctcattgc gattattatt tctgctatgg cagcctatgg tattgttcga ttctttccta
     3721 aattgggagc aatcatgtcg agactactcg tcattaccta cattttccca ccaattttgt
     3781 tagcaattcc ctattcaatt gccattgcta aagttgggtt aacaaatagt ttatttggct
     3841 tgatgatggt ttatctatct tttagtgttc catatgcagt ttggctctta gttggatttt
     3901 tccaaacagt tccaattgga attgaagaag cggctagaat tgatggtgca aataaatttg
     3961 ttacgtttta taaagttgtg ctaccgattg tagcaccagg tattgtagca acagctattt
     4021 atacatttat caatgcttgg aatgaattcc tgtatgcctt gattttgatt aacaatacag
     4081 gaaagatgac agtagcagta gcccttcgtt cacttaatgg ttcagaaata ctagactggg
     4141 gagatatgat ggcagcgtct gttattgtag ttcttccatc aattattttc ttctctatca
     4201 tccaaaataa gattgcaagt ggattatcag aaggatctgt gaagtagacg aaagaaggaa
     4261 aaaaatgaat aaaagaggtc tttattcaaa actaggaatt tctgttgtag gcattagtct
     4321 tttaatggga gtccccactt tgattcatgc gaatgaatta aactatggtc aactgtccat
     4381 atctcctatt tttcaaggag gctcatatca actgaacaat aagagtatag atatcagccc
     4441 tttgttatta gataaattgt ctggagagag tcagacagta gtaatgaaat ttaaagcaga
     4501 tgaaccaaac tctcttcaag ctttgtttgg cctatctaat agtaaagcag gctttaaaaa
     4561 taattacttt tcaattttca tgagagattc tggtgagata ggtgtagaaa taagagacgc
     4621 ccaaaaggga ataaattatt tattttccag accagcttca ttatggggaa aacataaagg
     4681 acaggcagtt gaaaatacac tagtatttgt atctgattct aaagataaaa catacacaat
     4741 gtatgttaat ggaatagaag tgttctctga aacagttgat acatttttgc caatttcaaa
     4801 tataaatggt atagataagg caacactagg agctgttaat cgtgaaggta aggaacatta
     4861 cctcgcaaaa ggaagtattg gtgaaatcag tctatttaac aaagcaatta gtgatcagga
     4921 agtttcaact attcccttat caaatccatt tcagttaatt ttccaatcag gagattctac
     4981 tcaagctaac tattttagaa taccgacact atatacatta agtagtggaa gagttctatc
     5041 aagtattgat gcacgttatg gtgggactca tgattctaaa agtaagatta atattgccac
     5101 ttcttatagt gatgataatg ggaaaacgtg gagtgagcca atttttgcta tgaagtttaa
     5161 tgactatgag gagcagttag tttactggcc acgagataat aaattaaaga atagtcaaat
     5221 tagtggaagt gcttcattca tagattcatc cattgttgaa gataaaaaat ctgggaaaac
     5281 gatattacta gctgatgtta tgcctgcggg tattggaaat aataatgcaa ataaagccga
     5341 ctcaggtttt aaagaaataa atggtcatta ttatttaaaa ctaaagaaga atggagataa
     5401 cgatttccgt tatacagtta gagaaaatgg tgtcgtttat gatgaaacaa ctaataaacc
     5461 tacaaattat actataaatg ataagtatga agttttggag ggaggaaagt ctttaacagt
     5521 cgaacaatat tcggttgatt ttgatagtgg ctctttaaga gaaaaacata atggaaaaca
     5581 ggtccctatg aatgttttct acaaagattc gttatttaaa gtgactccta ctaattatat
     5641 agcaatgaca actagtcaga atagaggaga gagttgggaa caatttaagt tgttgcctcc
     5701 gttcttagga gaaaaacata atggaactta cttatgtccc ggacaaggtt tagcattaaa
     5761 atcaagtaac agattgattt ttgcaacata tactagtgga gaactaacct atctcatttc
     5821 tgatgatagt ggtcaaacat ggaagaaatc ctcagcttca attccgttta aaaatgcaac
     5881 agcagaagca caaatggttg aactgagaga tggtgtgatt agaacattct ttagaaccac
     5941 tacaggtaag atagcttata tgactagtag agattctgga gaaacatggt cggaagtttc
     6001 gtatattgat ggaatccaac aaacttcata tggcacacaa gtatctgcaa ttaaatactc
     6061 tcaattaatt gatggaaaag aagcagtcat tttgagtaca ccaaattcta gaagtggccg
     6121 taagggaggt caattagttg tcggtttagt caataaagaa gatgatagta ttgattggaa
     6181 ataccactat gatattgatt tgccttcgta tggttatgcc tattctgcga ttacagaatt
     6241 gccaaatcat cacataggtg tactgtttga aaaatatgat tcgtggtcga gaaatgaatt
     6301 gcatttaagc aatgtagttc agtatataga tttggaaatt aatgatttaa caaaataaag
     6361 gagaaaaaca tggttaaata cggtgttgtt ggagcagggt attttggagc tgaattggct
     6421 cgctacatgc aaaagaatga tggagcagag attactcttc tctatgatcc agataatgca
     6481 gaggcgattg cagaagaatt gggagcaaaa gtagcaagtt ccttagatga gttggtttct
     6541 agcgatgaag tagattgtgt tatcgtcgca actccaaata atcttcataa ggaaccggtt
     6601 attaaggctg cacagcatgg taaaaatgtt ttctgtgaaa aaccaattgc gctttcttat
     6661 caagattgtc gcgagatggt agatgcgtgt aaagaaaaca atgtaacctt tatggcagga
     6721 catattatga atttctttaa tggtgttcat catgcaaaag aactcattaa tcaaggagtt
     6781 atcggagacg ttctatattg tcatacagct cgtaatggtt gggaagaaca acaaccgtca
     6841 gtatcatgga aaaaaattcg tgaaaaatca ggtggtcact tgtatcacca catccatgaa
     6901 ttggattgcg ttcaattcct tatggggggc atgcctgaaa ctgtaaccat gacaggtgga
     6961 aatgtggccc atgaaggtga acatttcggt gatgaagatg atatgatttt tgtcaatatg
     7021 gaattttcta ataagcgttt tgctttgtta gaatggggtt cagcttatcg ttggggtgaa
     7081 cattatgtct taatccaagg aagcaaaggt gccatccgct tagacttatt caactgtaaa
     7141 ggaactctta agctagatgg gcaagaaagc tatttcttga ttcacgaatc gcaagaagaa
     7201 gatgatgatc ggactcgtat ctatcatagt acagagatgg atggagcaat tgcttatggt
     7261 aaaccaggta aacgtactcc attatggcta tcatctgtca ttgataaaga aatgcgctat
     7321 ctgcatgaga ttatggaagg agctccagta tcagaagaat ttgcaaaact tttgacaggt
     7381 gaagctgccc tagaagcaat tgctactgca gatgcttgta cccagtctat gtttgaagat
     7441 cgcaaagtaa aattgtcaga aattgtaaaa taaattttgg tattctccta tttataggtc
     7501 gacttgctcc tctgaaagta cttttagagg agctgtttga ctttgctagt ttttgaaact
     7561 gaaatctatt atactacaaa ctattgaaag cgttttaatt ttaaggtata ataatctcat
     7621 agaaataaag aaaaggagga aagaggatgc cacagattag caaagaagcc ttgattgagc
     7681 aaatcaaaga tggaatcatc gtttcttgtc aggctcttcc tcatgaaccg ctttatacag
     7741 aagcgggagg ggtgattccc ttgctggtca aagcggctga gcaaggtgga gcagtcggta
     7801 tccgagcaaa cagtgttcgc gatatcaagg aaattaagga ggtcactaaa cttccaatca
     7861 ttgggattat caaacgtgat tatccacctc aggaaccctt catcacggct actatgaaag
     7921 aagttgatga attggcagaa ctggacatcg aggtgattgc tctggattgt accaagcgtg
     7981 aacgctacga tggtttggaa attcaagagt tcattcgtca ggttaaggag aaatatccta
     8041 atcagctttt gatggctgat actagtatct tcgaagaagg gctagcagct gtagaagcag
     8101 gaattgactt tgtcggaaca accttatcag gctacacatc ctacagtcca aaagtagacg
     8161 gtccagattt tgaattgatt aagaaactct gtgatgccgg tgtagatgtc attgcagaag
     8221 gaaaaattca tacaccagaa caagccaaac aaatccttga atatggagtg cgaggcatcg
     8281 ttgttggtgg cgccattact agaccaaaag agattacaga acgcttcgtt gctagtctta
     8341 aataagatgt gagggggagt tttatgttta aagttttaca aaaagttgga aaagctttta
     8401 tgttacctat agctatactt cctgcagcag gtctactttt ggggattggt ggtgcacttt
     8461 caaacccaac cacgatagca acttatccaa tactagacaa tagtattttt caatcaatat
     8521 tccaagtaat gagctctgca ggagaggttg tattcagtaa tttgtcacta cttctctgtg
     8581 tgggattatg tattggctta gcgaaacgag ataaaggaac cgctgcgtta gcaggagtaa
     8641 ctggttactt agttatgact gcaacgatca aagctttggt aaaacttttt atggcagaag
     8701 gatctgcaat tgatactgga gttattggag cattagctgt cggaatagtt gccgtatatt
     8761 tgcacaaccg atataacaat attcaattac cttccgcttt aggattcttt ggaggttcac
     8821 gcttcgttcc tattgttaca tcgttctctt ctatcttgat tggctttgtc ttctttgtta
     8881 tttggccacc tttccaacaa cttcttgttt ctacaggtgg atatatttct caggcgggtc
     8941 caattggaac ttttctatat ggatttttaa tgagactttc tggagcagta ggcttacatc
     9001 atataattta ccctatgttt tggtatactg aacttggtgg tgttgaaact attgcaggac
     9061 aaacagtggt tggagctcaa aaaatatttt ttgctcaatt agccgatccg gcccattctg
     9121 gattatttac agaaggaaca aggttttttg caggtcgttt ctcaacaatg atgttcggtt
     9181 taccggctgc ctgtttagcg atgtaccata gtgttcctaa aaatcgtcgt aaaaaatacg
     9241 cgggtttgtt ttttggagtt gctttaacat cttttattac cggtattaca gaaccaattg
     9301 aatttatgtt tctattcgtc agtccggttc tatatgttgt tcacgcattc cttgatggtg
     9361 ttagcttctt tattgcagac gtcttaaata tttcaatagg aaacacattt tcaggaggtg
     9421 taatcgattt cactttattt ggaattttgc aggggaacgc taagacgaat tgggttcttc
     9481 agattccaat tggacttatt tggagtgttt tgtattatat tatttttaga tggttcatta
     9541 ctcaattcaa tgttctaacg ccaggacgag gagaagaagt agattctaaa gaaatttctg
     9601 aatccgcaga ttcaacttca aatactgcag attatttaaa acaggatagc ctacaaatta
     9661 tcagagcctt gggtggatca aataatatag aagatgtaga tgcttgtgtg acacgtttac
     9721 gtgtagctgt aaaagaagtt aatcaagttg ataaagcact tttaaaacaa attggtgcag
     9781 ttgatgtctt agaagtgaag ggtggcattc aagcaatcta tggagcaaaa gcaatcttat
     9841 ataaaaatag tattaatgaa attttaggtg tagatgatta agtacttact gacttaataa
     9901 aaaacagagg agagtgatgg atgagtagga tgaaatgaaa tcgcatacaa gaaataaaga
     9961 actcattatc caagttggat acgcttatta cataggagaa tacaaatgaa atttagaaaa
    10021 ttagcttgta cagtacttgc gggtgctgcg gttcttggtc ttgctgcttg tggcaattct
    10081 ggcggaagta aagatgctgc caaatcaggt ggtgacggtg ccaaaacaga aatcacttgg
    10141 tgggcattcc cagtatttac ccaagaaaaa actggtgacg gtgttggaac ttatgaaaaa
    10201 tcaatcatcg aagcgtttga aaaagcaaac ccagatataa aagtgaaatt ggaaaccatc
    10261 gacttcaagt caggtcctga aaaaatcaca acagccatcg aagcaggaac agctccagac
    10321 gtactctttg atgcaccagg acgtatcatc caatacggta aaaacggtaa attggctgag
    10381 ttgaatgacc tcttcacaga tgaatttgtt aaagatgtca acaatgaaaa catcgtacaa
    10441 gcaagtaaag ctggagacaa ggcttatatg tatccgatta gttctgcccc attctacatg
    10501 gcaatgaaca agaaaatgtt agaagatgct ggagtagcaa accttgtaaa agaaggttgg
    10561 acaactgatg attttgaaaa agtattgaaa gcacttaaag acaagggtta cacaccaggt
    10621 tcattgttca gttctggtca agggggagac caaggaacac gtgcctttat ctctaacctt
    10681 tatagcggtt ctgtaacaga tgaaaaagtt agcaaatata caactgatga tcctaaattc
    10741 gtcaaaggtc ttgaaaaagc aactagctgg attaaagaca atttgatcaa taatggttca
    10801 caatttgacg gtggggcaga tatccaaaac tttgccaatg gtcaaacatc ttacacaatc
    10861 ctttgggcac cagctcaaaa tggtatccaa gctaaacttt tagaagcaag taaggtagaa
    10921 gtggtagaag taccattccc atcagacgaa ggtaagccag ctcttgaata ccttgtaaac
    10981 gggtttgcag tattcaacaa taaagacgac aagaaagtcg ctgcatctaa gaaattcatc
    11041 cagtttatcg cagatgacaa ggagtgggga cctaaagacg tagttcgtac aggtgctttc
    11101 ccagtccgta cttcatttgg aaaactttat gaagacaaac gcatggaaac aatcagcggc
    11161 tggactcaat actactcacc atactacaac actattgatg gatttgctga aatgagaaca
    11221 ctttggttcc caatgttgca atctgtatca aatggtgacg aaaaaccagc agatgctttg
    11281 aaagccttca ctgaaaaagc gaacgaaaca atcaaaaaag ctatgaaaca atagtcctta
    11341 gttattctat aaaaagtagt tttttaaaga acctaagagt gtataccccc ttttccctct
    11401 acacagatag tgtaagaaaa gggggctttt gtttaaaatg taagaaactg tcacgaaatt
    11461 aaaatgaagt tcttacataa gcgaatcata aaaaatttca ttttgatttt aaaacagttc
    11521 aagaaagtca aaaaattatt ctatttgaaa gagaggtgcc gactgtgaaa gtcaataaaa
    11581 tccgtatgcg ggaaacagtg atttcctacg ctttcctagc accagtatta ttcttctttg
    11641 tcatctttgt gttggctccg atggtgatgg gcttcattac aagtttcttt aactactcaa
    11701 tgactaaatt tgagtttgta ggcttggata actatatccg tatgtttaaa gatcctgtct
    11761 ttacaaaatc tctgattaac acagttattt tggttattgg atctgtacca gttgttgttc
    11821 tattctcact ctttgtagca tctcagacct atcatcaaaa tgtcattgcc agatccttct
    11881 accgtttcgt cttcttcctt cctgttgtaa cgggtagtgt tgccgtgaca gttgtttgga
    11941 aatggattta tgacccacta tcagggattc taaactttgt ccttaagtcc agccacatca
    12001 tcagccaaaa catttcttgg ttgggagata aaaactgggc attgatggcg attatgatta
    12061 ttctcttgac cacttcagtt ggtcagccca tcatccttta tatcgctgcc atggggaata
    12121 ttgacaattc actggttgaa gcggcgcgtg ttgatggtgc aactgagttt caagtttttt
    12181 ggaagattaa atggccaagc cttcttccaa caactcttta tattgcaatc atcacaacaa
    12241 ttaactcatt ccagtgtttt gccttgattc agcttttgac atctggtggt ccaaactact
    12301 caacaagtac cttgatgtac tacctttacg aaaaagcctt ccaattgaca gaatacggct
    12361 atgccaacac aattggtgtc ttcttggcaa tcatgattgc tatcgtaagc tttgttcaat
    12421 ttaaagtact tggaaacgac gtagaatact aaagaaagga gacagctatg caatctacag
    12481 aaaaaaaacc attaacagcc tttactgtta tttcaacaat cattttgctc ttgttgactg
    12541 tgctgttcat ctttccattc tactggattt tgacaggggc attcaaatca caacctgata
    12601 caattgttat tcctcctcag tggttcccta aaatgccaac catggaaaac ttccaacaac
    12661 tcatggtgca gaaccctgcc ttgcaatgga tgtggaactc agtatttatc tcattggtaa
    12721 ccatgttctt agtctgtgca acctcatctc tagcaggtta tgtattggct aaaaaacgtt
    12781 tctatggtca acgcattcta tttgctatct ttatcgctgc tatggcgctt ccaaaacaag
    12841 ttgtccttgt accattggta cgtatcgtca acttcatggg aatccacgat actctctggg
    12901 cagttatctt gcctttgatt ggatggccat tcggtgtctt cctcatgaaa cagttcagtg
    12961 aaaatatccc tacagagttg cttgaatcag ctaaaatcga cggttgtggt gagattcgta
    13021 ccttctggag tgtagccttc ccgattgtga aaccagggtt tgcagccctt gcaatcttta
    13081 ccttcatcaa tacttggaat gactacttca tgcaattggt aatgttgact tcacgtaaca
    13141 atttgaccat ctcacttggg gttgcgacca tgcaggctga aatggcaacc aactatggtt
    13201 tgattatggc aggagctgcc cttgctgctg ttccaatcgt cacagtcttc ctagtcttcc
    13261 aaaaatcctt cacacagggt attactatgg gagcggtcaa aggataatac tctgagaaaa
    13321 tctcttcaaa ctacgtcagc gtcaccttgc catacttaag tattgcctgc ggttagcttc
    13381 ctagtttgtt cttcaatttt cattgagtat aggaaaatca atctatcaag atacagaagt
    13441 atattttata gatttagaga atatagaggt tataagtgtc tacaaaatgg agggtatgca
    13501 gttactttat gaagttttgt cagacactta taaacttaag aatggtttta gttaactatc
    13561 agaaacgaag gaaagagtat gatttttgac gatttgaaaa acatcacctt ttacaaaggg
    13621 attcatccca atttagacaa ggctatcgac tatctctacc aacatcgtaa agattcattc
    13681 gaattaggaa agtatgagat tgatggagat aaagtctttc tagttgttca ggaaaatgtc
    13741 ctcaatcaag ttgagaataa tcgatttgaa caccataaga actatgcaga tttgcatttg
    13801 ctgatagaag ggcatgaata ttcgagctac ggttcacgta tcaaagacga ggcagtagca
    13861 ttcgacgaag cgagtgacat tggctttgtt cattgtcatg aacactaccc actcttgttg
    13921 ggttatcaca attttgcgat tttcttccca ggtgagccac atcagccaaa tggttatgca
    13981 ggcatggaag aaaaggttcg aaaatatctc tttaaaattt tgattgatta aaaataggat
    14041 gaattgtttt tttgtaaagc tttgataata ctctaccatg aaattgatct ttgtgaggta
    14101 gagaaatgag aataaaatat ttaaaaattg gtatcttcta agtatgctgc aagagctagt
    14161 ttcttagatg gacaggggat tacagttgat gagatggctt ggataattag gggcattgtg
    14221 actgcattga ttggtagata cataaaatta ggtacttatg cagttaagta tggtattagt
    14281 atggcgcgct cgatcttaag tagggcagct gcaactgcgg cagcaagagt aggattattg
    14341 actaagattt ttggatggat tttacgaata gctgtgctcg cagctgatgt atatggtaat
    14401 tttgccaaca atattgctgt agcttgggat gcacatgata aaatccctaa caatggtcgt
    14461 ataaactttt aaaatgcgag aatgaaagca ctttgtattt ttttattgta tatgttagct
    14521 tggacagtgc ttgcaatgat aatccgtgga gggctagatg gatttgatga acacacttgg
    14581 cgtactattt taattgcttc gctgtccggg atatatatag attatatgtc catagataaa
    14641 agtagaaaaa agtccaaaag aaaaaagaaa aaatagattt gttcatggta gggacttatg
    14701 aaagctttac tgacaaaaaa gaaaacagtt tacaaagaaa aatgatggag gagcaaacat
    14761 ggcacaaaaa ggagtaagcc ttatcaaggc agcatttgat acagataact ttctcatgcg
    14821 ttttagtgag aaagtcttgg acatcgtgac agccaatctt ctttttgtcg tctcttgttt
    14881 gcccatcgtg acgattggag tggctaaaat cagcctctac gagaccatgt tcgaagttaa
    14941 gaagagcaga cgggtgcctg tttttaaaat ctatctaaga tctttcaagc aaaatctgaa
    15001 actaggtctt cagctgggtt taatggagtt aggaattgtg tttcttaccc tttcagatct
    15061 ctatcttttc tggggtcaaa cagctctgcc cttccaattg ctgaaagcca tttgtctagg
    15121 cattctgatt ttccttacga ttgtgatgtt ggctagctat ccaatcgcgg cacgttatga
    15181 tttaacttgg aaagaagtgt tacaaaaagg gcttatcttg gctagtttta actttccttg
    15241 gttcttcctc atgttagcca ttcttgtcct cattgtgatg gttctttatc tgtccgcctt
    15301 cagtctactc ttaggtggct cagtcttcct actttttggg tttggactat tggtctttat
    15361 ccagactgga ttgatggaga aaattttcgc aaaataccaa taggagcctt atttctgaaa
    15421 ctactttcaa aggctccaaa cgctattcta taagcgagaa actaaaatct aagtgtctat
    15481 aagatattga aagccttttc ggtaaagtgt atactaaaga agtaaagaaa aatctgcttt
    15541 agcagaaaat aaaaataata ggagaaaatc tatgtcagat ttgaaaaaat acgaaggtgt
    15601 cattccagcc ttctacgcat gttatgatga gcaaggagaa gtaagtccag agcgtacgcg
    15661 tgccttggtt caatacttca ttgataaagg tgttcaaggt ctttatgtca atggttcttc
    15721 tggtgaatgt atctaccaaa gcgttgaaga tcgcaagttg attttggaag aagtcatggc
    15781 ggtagccaaa ggtaaattga ccattattgc ccatgttgct tgcaataata ctaaagatag
    15841 tatggaactt gctcgccatg ctgaaagctt gggagtagat gctattgcaa cgattccacc
    15901 aatttacttc cgcttgccag aatactcagt tgccaaatac tggaacgata ttagttctgc
    15961 agctccaaac acagactacg tgatttacaa cattcctcaa ttggcagggg ttgctttgac
    16021 ttcaagcctt tacacagaaa tgttgaaaaa tcctcgtgtt atcggtgtta agaactcttc
    16081 aatgccagtt caagatatcc aaacctttgt cagccttggc ggggaagacc atattgtctt
    16141 taacggtcca gatgaacagt tcctaggtgg ccgcctcatg ggtgctaagg ctggtatcgg
    16201 tggtacttat ggtgctatgc cagaactctt cttgaaactc aatcagttga ttgcggataa
    16261 agatttagaa acagcgcgtg aattgcagta tgctatcaac gcaatcattg gtaaactcac
    16321 tgctgcacat ggaaatatgt acggtgtcat caaagaagtt ttgaagatca atgaaggctt
    16381 gaatattggt tctgttcgtt caccattgac accagtgact gaagacgatc gtccagttgt
    16441 agaagcggct gcagccttga ttcgtgaaac caaggagcgc ttcctctaat ctaaaaggag
    16501 gtatttatga catactacgt tgcaattgat atcggtggaa ctaacatcaa gtatggtttg
    16561 gttgatcaag aggggcaact tcttgaatca catgaaatgc caacagaggc gcataagggt
    16621 ggacctcata tcttacaaaa gaccaaagat atcgtagcta gttatttaga aaaaggccca
    16681 gtagcaggtg ttgccatatc ttctgctggg atggtggatc cggataaggg tgagattttc
    16741 tatgctgggc cgcaaatccc taactacgca ggcacccagt tcaaaaagga aatcgaagaa
    16801 agctttacta ttccttgtga gattgaaaat gatgtcaact gtgcaggtct tgctgaggca
    16861 gtatctggtt caggcaaggg agcaagtgtg acactttgct tgaccattgg aaccggtatc
    16921 ggtggttgct tgattatgga taggaaagtc ttccatggtt ttagtaattc agcctgtgaa
    16981 gtcgggtata tgcatatgca ggatggagct tttcaggatc tcgcttctac aacagcttta
    17041 gtgaaatatg tagctgaagc ccatggagaa gatgttgatc agtggaatgg ccgacgtatc
    17101 tttaaggaag ctactgaagg aaacaaaatc tgcatggaag gtattgaccg tatggttgac
    17161 tatctaggaa aaggtctggc aaatatttgc tacgttgcca atccagaagt ggtcattctt
    17221 ggtggtggta tcatggggca agaggctatc ctcaaaccta agatccgcac agccttgaaa
    17281 gaggctttgg taccaagttt agcagaaaaa acacgattag aatttgccca tcaccaaaat
    17341 acagcaggga tgttgggtgc atattatcat tttaagacaa aacaatccta gtttggctca
    17401 gccaaactag gattttctta cacgtttttg tctacgatag ccgttgagtt ttttattttc
    17461 ccagtagcta ttaaagattt tttccttgct ttcgcgattg atttccaaaa agtaggcata
    17521 aatcaaatcg ataaagaaga gcataggaag ttgagcggat attcgttgga tataggaggg
    17581 ttggctgtgg gtggctacaa gaacagtctc tgtataggtc tggctatttt tattgggaac
    17641 acttgtaaag agtacagtct ttgcccccat ctccttggca tctaatagac tatctaaaat
    17701 agaaggagtt gagcctgaaa gtgagaagcc cagtactaga caattttcat ccatgatgct
    17761 ggttgtccag gcaaagccgt cttggtctgt caaagcttcg cagaccacac ctaatcgcat
    17821 aaaacgtaat ttcatttcac gggcgacgag gccagaactc cctgttccaa agaagtagat
    17881 acgctcagca tcttcgatta gctgggcaat tcgttctagt tggatttcgt caatcaagtc
    17941 ttgtgtttgt tccctcatat tgctataact tctgaggact cgtttggtca gtggactgtg
    18001 cttggaaact tggttggctt gattttctgc ctgatgttgg tattggaaaa taaattctcg
    18061 gtagccagta aagccacact ttttagcaaa gcgggtcaaa gcagcttgag aaatatgtaa
    18121 tttttgggtg acttgttgag aagataaatc atctgtaatc gtttcagctt gcaaaaaata
    18181 gcgagcgatt tcttgctcta ggtctgtcat ttcttcaaaa tgtgaatcaa tgatagttgc
    18241 gatatctggt ttgtccatag ggaaagctcc tttacatgag tcatactgga agactagatc
    18301 agggaatagt cacacttcat tataacacat aatataagga tagataaata aaaacgcatc
    18361 tctgttttaa aaacgaaaaa atcgaaaaag cttctctctt ttccataatt ttctactcaa
    18421 attgtggtac aattaagagt aagattttaa gttagaaatg agactgattt gtatgagaaa
    18481 atttaacagc cattcgattc cgattcggct taatttattg ttttcaatcg tcattttact
    18541 ctttatgacc attattggtc gtttgttgta tatgcaggtt ttgaacaagg atttttacga
    18601 aaaaaagcta gcctcagcta gtcagaccaa gattacaagc agttcagccc gtggggaaat
    18661 ttatgatgct agtggaaaac ctttggtaga aaatacgtta aagcaggttg tttcctttac
    18721 gcgtagcaat aaaatgacgg ctacagactt aaaagaaaca gctaaaaagt tactgactta
    18781 tgtgagcatc agttctccaa atttgacaga acgccagctg gcggattact atttggctga
    18841 tcctgaaatc tataaaaaaa tagtggaagc tctcccaagc gagaaacgct tggattcaga
    18901 tggcaatcgt ctatccgaat cagaactgta taacaatgcg gtcgatagtg tacaaacgag
    18961 tcaactaaac tatacagagg atgaaaagaa agaaatctat ctttttagtc agttaaatgc
    19021 tgttggaaac tttgcgacag gaaccattgc tacagatcct ctaaatgatt ctcaggtggc
    19081 tgttattgcc tctatttcaa aggagatgcc tggcattagt atttctactt cttgggatag
    19141 aaaggttttg gaaacttccc tttcttctat agttgggagt gtatccagtg aaaaagctgg
    19201 tctcccagcg gaagaagcag aagcctatct taaaaaaggc tattctctaa atgatcgtgt
    19261 aggaacctcc tatttggaaa agcaatatga agagacctta caaggaaaac gctcggtaaa
    19321 agaaatccat ctggataaat atggcaatat ggaaagcgtg gatacaattg aggaaggtag
    19381 taagggaaac aatatcaaac tgaccattga tttggctttc caagatagcg tggatgcttt
    19441 actgaaaagt tatttcaatt ctgagctaga aaatggtgga gccaagtatt ctgaaggtgt
    19501 ctatgcagtc gcccttaacc caaaaacagg tgcggttttg tctatgtcag ggattaaaca
    19561 tgacttgaaa acgggagagt tgacgcctga ttccttggga acggtaacca atgtctttgt
    19621 tccaggttcg gttgtcaagg cggcgaccat cagctcaggt tgggaaaatg gagtcttgtc
    19681 aggaaaccag accttgacag accagtccat tgtcttccaa ggttcagctc ccatcaattc
    19741 ttggtatact caggcttacg gttcattccc tatcacagcg gtccaagctc tggagtattc
    19801 atcaaatacc tatatggtcc aaacagcctt aggtcttatg gggcaaacct atcaacccaa
    19861 tatgtttgtc ggcaccagca atctagagtc tgctatggag aaactgcgtt caacctttgg
    19921 cgaatatggc ttgggtactg cgacaggaat tgacctacca gatgaatcta ctggatttgt
    19981 tcccaaagag tatagctttg ctaattacat tactaatgcc tttgggcagt ttgataacta
    20041 tacgccgatg cagttggctc agtatgtagc aactattgca aataatggtg ttcgtgtggc
    20101 tcctcgtatt gttgaaggca tttatggtaa taatgataag ggaggactgg gtgacttgat
    20161 tcagcaactg caaccgacag agatgaataa ggtcaatata tccgactccg atatgagcat
    20221 cttgcaccaa ggtttttatc aggttgccca tggtactagt ggattgacaa ctggacgtgc
    20281 cttttcaaat ggtgccttgg tatccattag cggaaaaaca ggtacagccg aaagctatgt
    20341 ggcagatggt cagcaagcaa ccaataccaa tgcggtggcc tatgccccat ctgataatcc
    20401 ccaaatcgct gtcgcagtgg tctttcctca taataccaat ctaacaaatg gtgtaggacc
    20461 ttccattgcg cgtgacatta tcaatctgta tcaaaaatac catccaatga actagaaagg
    20521 aaattatgct ttatccaaca cctattgcca agttgattga cagttattct aagttaccag
    20581 gtatcgggat taagacggct acgcgtctgg ccttttatac gattgggatg tctgctgatg
    20641 atgtcaatga atttgcaaaa aatctccttt ctgctaagag agaattgaca tattgttcta
    20701 tttgtggacg tttgacagac gacgatcctt gttctatctg tactgattcg actcgtgacc
    20761 agacaacaat tttagttctt gaggatagta gagatgtggc agccatggaa aatatccaag
    20821 aataccatgg actctatcat gtcctgcatg gcctcatttc tcctatgaat ggtatcagtc
    20881 cggacgatat caatcttaag agccttatga ctcgtcttat ggatagtgag gtttcagaag
    20941 tgattgtggc gactaatgct acagcggatg gtgaagcgac ctccatgtat ctttcacgtt
    21001 tgctcaagcc ggctggtatc aaggttacgc gtctagcacg aggtctcgct gtgggagcgg
    21061 acattgagta tgcggacgaa gtgacactct tacgagcgat tgaaaatcgg acagagttgt
    21121 aagtgtaggc aaatttacga actccattca tttataaaaa atcaaagagg ctgaaaatcg
    21181 ttcctatcgg cctctttttg tatagtgtga tgagtagaat caggttcaag ttttaaaaaa
    21241 ccaagcaaat atgatatact aaagagcgag tattctagta gaattaggac aaataatatg
    21301 aaacaaacga ttattctttt atatggtgga cggagtgcgg aacgcgaagt ctctgtcctt
    21361 tcagctgaga gtgtcatgcg tgcggtcaat tacgaccgtt tcacagtcaa gactttcttt
    21421 atcagtcagt caggtgactc tatcaaaacg caggaattta gtcatgctcc ggggcaagaa
    21481 gaccgtctca tgaccaatga aaccattgat tgggataaga aagttgcacc aagtgctatc
    21541 tacgaagaag gtgcagtggt ctttccagtc cttcacgggc caatgggaga agatggctct
    21601 gttcaaggat tcttggaagt tttgaaaatg ccttacgttg gttgcaacat tttgtcatca
    21661 agtcttgcca tggataaaat cacgactaag cgtgttctgg aatctgctgg tattgcccaa
    21721 gttccttatg tggctatcgt tgaaggcgat gatgtgactg ctaaaatagc tgaagtggaa
    21781 gaaaaattgg cttatccagt cttcactaag ccgtcaaaca tggggtctag tgtcggtatt
    21841 tctaagtctg aaaaccaaga agaactccgt caagccttaa aacttgcctt ccgatatgac
    21901 agccgtgtct tggttgagca aggagtgaat gcccgtgaaa ttgaggttgg cctcttgggt
    21961 aactacgatg tcaagagcac gctacctgga gaagttgtca aggacgttgc cttttatgac
    22021 tacgatgcca agtatattga taacaaggtt actatggata ttcctgccaa aatcagtgat
    22081 gatgtggtgg ctgtcatgcg tcaaaatgca gaaacagcct tccgtgccat tggtggcctt
    22141 ggtctatctc gttgcgattt cttctataca gataagggag agatttttct caacgagctc
    22201 aataccatgc caggtttcac ccagtggtct atgtacccac tactttggga aaatatgggg
    22261 atcagctacc cagaactaat cgagcgtttg gttgaccttg ccaaggaaag ttttgacaag
    22321 cgcgaagcgc atttgatata aaatgaaaga gagggtagaa gccagaacca tcactgcaag
    22381 gtgactagag ttctcggact tcagcccttt ttaaaggagt agaaatgaaa ttaacaatcc
    22441 atgaaattgc ccaagttgtt ggagccaaaa atgatatcag tatctttgag gacactcagt
    22501 tagaaaaagc tgagtttgat agtcgtttga ttggaactgg agatttattt gtgccactta
    22561 aaggtgcgcg tgatggccat gactttattg aaacagcctt tgaaaatggt gcagcagtaa
    22621 ccttgtctga gaaagaggtc tcaaatcatc cttacatttt agtagatgat gttttgacag
    22681 cctttcaatc cctagcatcc tactatcttg aaaaaacgac tgttgatgtc tttgctgtta
    22741 caggttcaaa tggcaagaca acgactaagg atatgttggc gcatttactg tcaacaagat
    22801 acaagaccta caaaacacaa ggcaattaca ataatgagat tggccttcct tacacagttc
    22861 ttcatatgcc tgaaggaaca gaaaagttgg ttttggagat gggacaggat cacttgggag
    22921 atattcatct cttgtctgaa ttggctcgtc caaaaacagc catcgtgacc ttggttggag
    22981 aagctcattt ggcctttttc aaagatcgtt ctgagattgc taaaggaaaa atgcaaattg
    23041 cagacggaat ggcttcaggt tccttgcttt tagcgccggc tgaccctatc gtagaggact
    23101 atttgccaat tgataaaaag gtggttcgtt ttggtcaagg agcagagctg gaaattactg
    23161 acttggttga gcgtaaggac agtctgacct ttaaggctaa tttcttggaa caagcccttg
    23221 atttgccagt aactggcaag tacaatgcga caaatgctat gattgcatcc tatgttgcct
    23281 tgcaagaagg agtttcagag gagcaaattc gtttggcatt ccaacatctt gaattgacgc
    23341 gtaaccgtac cgagtggaag aaagcagcca atggagcaga tatcctgtca gatgtttaca
    23401 atgccaatcc aactgctatg aaactgattt tagagacttt ctctgccatt ccagccaatg
    23461 aaggtggcaa gaaaattgca gtgttggcgg atatgaagga gcttggtgac cagtctgttc
    23521 aacttcataa tcagatgatt ttgagcctct ccccagatgt gcttgatatc gttattttct
    23581 atggagaaga tattgctcag ttggctcaat tggccagtca aatgtttcca atcggccacg
    23641 tttactactt caagaaaaca gaagaccagg atcaatttga agacctagtc aagcaggtta
    23701 aggaaagcct tggagcctat gaccaaatct tgctaaaagg ttctaactct atgaatctag
    23761 ccaaattggt agaaagttta gaaaatgaat gcaagtgatt ttgctaagta tcttcaaaga
    23821 atgattgctc ttacagatac tggactaacc tttacaaagg atccgtttga ccgtgagcgc
    23881 tacgaagact tgcgaagtct gttatctgaa atgttgaatc aggtatcaga ccttgatgca
    23941 gacgaagtgg cagaactctt gaaaccgacc tccgcttatg cgactccttt aatggacgtc
    24001 cgtgcttgga tagttgagga tgaaaaaatt tgtctggtta ggggacaagg agagaatgat
    24061 tgggctttgc caggtggctt tggcgaggtc ggttattcac caactgaaaa tattctcaag
    24121 gaaattgaag aagaaaccgg ttttaaagcc aaagttgaac gactactagc tgtttttgat
    24181 accaatcgtt tccaactaca gagcaaacaa tatgcaaagt ttgtttttga atgtaagctt
    24241 ctcgatggac aatttcaaga aaatcaagaa atcgctgacc ttcaattttt tgcaattgac
    24301 caactgccga atttatctga aaaacgcatt accaaggagc aaatagagct tctttggcag
    24361 gtttatcaag gtcatagggg gcaatatctt gactaagaag atgattatcg tatttctaaa
    24421 tccattttta acaactagca tggtataata atatgcagga aaattttgaa ttatgaggaa
    24481 gactagatga atttatggga tattttcttt acgactcagg caaccgagcc gcccaaattt
    24541 gacctttttt ggtatgttag cctatttacg ctcttagcct taacctttta tacagcccat
    24601 cgctatcgtg aaaataaggt ttaccaacga tttttccaaa tcttgcagac tgttcagtta
    24661 atccttcttt atggttggta ctgggtcaat catatgccac tgtcagaaag cctacccttt
    24721 taccattgcc gtatggctat gtttgtggta ctcttgcttc ctggtcaatc caaatataaa
    24781 caatactttg cattattggg aacatttggg acattagcag cctttgttta tccagtgcca
    24841 gatgcttacc cttttccaca tatcaccatt ctatccttta tctttggtca tttagcactc
    24901 ttggggaact ctctagttta tctattgaga cagtataatg cgcgattgct ggatgtgaag
    24961 ggaatttttc tcatgacctt tgccctaaat gccttgattt ttgtggtcaa tttggtgaca
    25021 ggtggcgatt acggattttt gacaaaaccg ccattggttg gggatcacgg tctagtagct
    25081 aattatttac ttgtttcaat tgtgctggta gctactatca gtttgactaa gaaaatctta
    25141 gaattctttt tagctcaaga agcagaaaaa atgattgcaa aggaagctta acacagagct
    25201 ttcttttttg ctcttagaga gtttttacaa gcagcttata aaataagaat ttctgaatag
    25261 acaaactcaa aaaatggctg ggaaatttag gaaaaaagca agcacgatta aattttttgt
    25321 gttataatat tttgtgaata gctatgccta tgtttagcta tggaataata cgaagtgcga
    25381 aacttggaag atagagagga agcgatgtaa tggctagaga aggctttttt acaggtctag
    25441 atattggaac aagctctgtc aaggtgcttg tggccgagca gagaaatggt gaattaaatg
    25501 taattggcgt gagtaatgcc aaaagtaaag gtgtaaagga tggaattatt gttgatattg
    25561 atgcagcagc aactgctatc aagtcagcca tttcccaagc ggaagaaaag gcaggcattt
    25621 cgattaaatc agtgaatgtc ggcttgcctg gtaatctttt gcaggtagaa ccaactcagg
    25681 ggatgattcc agtaacatct gatactaagg aaattacgga tcaagatgtt gaaaatgttg
    25741 tcaaatcagc tttgacaaag agtatgacac ctgaccgtga agtcattacc tttattcctg
    25801 aagaatttat tgtggatggt ttccaaggga ttcgtgaccc acgtggcatg atgggggttc
    25861 gccttgaaat gcgtggtttg ctttatacag gacctcgtac tatcttgcac aatttgcgta
    25921 agacggttga gcgtgcaggt gttcaggttg aaaatgttat catttcacca ctagcaatgg
    25981 ttcagtctgt tttgaacgaa ggggaacgtg aatttggtgc tacagtgatt gatatggggg
    26041 caggtcaaac gactgtcgct acaatccgta atcaagaact ccagttcaca catattctcc
    26101 aagaaggtgg agattatgta actaaagata tctccaaggt tttgaaaacc tctcgcaaat
    26161 tagcggaagg cttaaaactg aattacgggg aagcctatcc gcctcttgca agcaaagaaa
    26221 ccttccaagt agaggttatt ggagaagtag aagcagtcga agtgacggaa gcctacttgt
    26281 cagaaattat ttctgcacga atcaagcaca tccttgaaca aatcaagcaa gaattagata
    26341 gaaggcgtct attggacctc cctggtggta ttgtcttaat cggtgggaat gccattttac
    26401 caggtatggt tgagcttgct caggaagtct ttggcgtccg tgtcaagctt tatgttccaa
    26461 atcaagttgg tatccgtaat ccagcctttg cgcatgtgat tagtttatca gaatttgcgg
    26521 gtcaattaac agaagttaat cttttggctc agggagcgat aaaaggtgag aatgacttaa
    26581 gtcatcagcc aattagtttt ggtgggatgc tgcaaaaaac agctcagttt gtacaatcaa
    26641 cgcctgttca accagctcct gctccagaag tagagccggt ggcgcctaca gaaccaatgg
    26701 cggatttcca acaagcttca caaaataaac cgaaattagc agatcgtttc cgtggcttga
    26761 tcggaagcat gtttgacgaa taaagaggaa aaataaatta tgacattttc atttgataca
    26821 gctgctgctc aaggggcagt gattaaagta attggtgtcg gtggaggtgg tggcaatgcc
    26881 atcaaccgta tggtcgacga aggtgttaca ggcgtagaat ttatcgcagc aaacacagat
    26941 gtacaagcat tgagtagtac aaaagctgag actgttattc agttgggacc taaattgact
    27001 cgtggtttgg gtgcaggagg tcaacctgag gttggtcgta aagccgctga agaaagcgaa
    27061 gaaacactga cggaagctat tagtggtgcc gatatggtct tcatcactgc tggtatggga
    27121 ggaggctctg gaactggagc tgctcctgtt attgctcgta tcgccaaaga tttaggtgcg
    27181 cttacagttg gtgttgtaac acgtcccttt ggttttgaag gaagtaagcg tggacaattt
    27241 gctgtagaag gaatcaatca acttcgtgag catgtagaca ctctattgat tatctcaaac
    27301 aacaatttgc ttgaaattgt tgataagaaa acaccgcttt tggaggctct tagcgaagcg
    27361 gataacgttc ttcgtcaagg tgttcaaggg attaccgatt tgattaccaa tccaggattg
    27421 attaaccttg actttgccga tgtgaaaacg gtaatggcaa acaaagggaa tgctcttatg
    27481 ggtattggta tcggtagtgg agaagaacgt gtggtagaag cggcacgtaa ggcaatctat
    27541 tcaccacttc ttgaaacaac tattgacggt gctgaggatg ttatcgtcaa cgttactggt
    27601 ggtcttgact taaccttgat tgaggcagaa gaggcttcac aaattgtgaa ccaggcagca
    27661 ggtcaaggag tgaacatctg gctcggtact tcaattgatg aaagtatgcg tgatgaaatt
    27721 cgtgtaacag ttgtcgcaac gggtgttcgt caagaccgcg tagaaaaggt tgtggctcca
    27781 caagctagat ctgctactaa ctaccgtgag acagtgaaac cagctcattc acatggcttt
    27841 gatcgtcatt ttgatatggc agaaacagtt gaattgccaa aacaaaatcc acgtcgtttg
    27901 gaaccaactc aggcatctgc ttttggtgat tgggatcttc gccgtgaatc gattgttcgt
    27961 acaacagatt cagtcgtttc tccagtcgag cgctttgaag ccccaatttc acaagatgaa
    28021 gatgaattgg atacacctcc atttttcaaa aatcgttaag taaatgaatg taaaagaaaa
    28081 tacagaactt gtttttcgag aagttgcaga ggctagtctg agtgctcatc gagagagtgg
    28141 ttcggtctct gtcattgcag ttaccaagta tgtagatgta ccgacagcgg aagccttgct
    28201 tccgctaggt gttcatcata tcggtgaaaa tcgtgtagat aagtttctgg aaaaatatga
    28261 agctttaaaa gatcgagatg tgacttggca tttgattggt accttgcaaa gacgtaaggt
    28321 gaaagatgtc attcaatacg ttgattattt ccatgcattg gactcagtaa agctagcagg
    28381 ggaaattcaa aaaagaagtg accgagtcat caagtgtttc cttcaagtaa atatttctaa
    28441 agaagaaagc aaacacggtt tttcgagaga ggaactgctg gaaatcttgc cagagttagc
    28501 cagactagat aagattgaat atgttggttt aatgacgatg gcaccttttg aggctagcag
    28561 tgagcagttg aaagagattt tcaaggcggc ccaagattta caaagagaaa ttcaagagaa
    28621 acaaattcca aatatgccta tgaccgagtt aagtatggga atgagtcgtg attataaaga
    28681 agcgattcaa ttcggttcca cttttgttcg tataggtaca tcatttttta agtaggagag
    28741 aaccatgtct ttaaaagata gattcgatag atttatagat tattttacgg aggatgagga
    28801 ttcaagtctc ccttatgaaa aaagagatga gcctgtgttt actccagtaa attcttcaca
    28861 ggaaccggct ctcccaatga atcaaccttc acagtcggct ggtacaaaag agaacaatat
    28921 caccagactt catgcaagac aacaggaatt ggcaaatcag agtcagcgtg caacggataa
    28981 ggtcattata gatgttcgtt atcctagaaa atatgaggat gcaacagaaa ttgttgattt
    29041 attggcagga aacgaaagta tcttgattga ttttcagtat atgacagagg tgcaggctcg
    29101 tcgttgtttg gactatttgg atggagcttg tcatgtttta gctggaaatt tgaaaaaggt
    29161 agcttctacc atgtatttgt tgacaccagt taatgttatt gtaaatgttg aagatatccg
    29221 tttaccagat gaagatcaac agggtgagtt cggttttgat atgaagcgaa atagagtacg
    29281 ataatgattt ttttaattcg tatgatttat aatgcagtgg atatttactc cctgattttg
    29341 gtagccttcg ctgtcatgtc ttggtttcca ggtgcctacg aatccagttt aggtcgttgg
    29401 attgtagcgt tggtgaaacc agtacttgct cccttgcaac gcctgccttt acagatagcg
    29461 ggtcttgatt tatctgtttg ggtcgcgatt gttttggttc gatttttagg agaaaaccta
    29521 gtgcgttttc tggcgatgat aggatgaata aagggattta tcagcatttc tccatagaag
    29581 atcgtccatt tcttgacaag ggaatggaat ggataaagaa ggtagaagat agctatgctc
    29641 cttttttaac tccttttatc aatcctcatc aggagaagct attaaagatt ttggccaaaa
    29701 cctatggtct tgcttgtagc agtagtgggg aattcgtctc gagtgagtat gttcgagttt
    29761 tattataccc agattatttc caaccagagt tttcagattt tgaaatatct ctccaagaaa
    29821 ttgtgtattc caataaattt gaacatttaa cgcatgctaa gattttaggg acagtcatca
    29881 atcaattagg gattgaacgg aaactttttg gagatatcct agtagatgaa gaacgggcgc
    29941 agattatgat taatcagcag tttcttcttc tctttcaaga tggactaaag aaaattggtc
    30001 gtatacctgt ttcgctggag gaacgtcctt tcaccgagaa aatagataag ctagaacagt
    30061 atcgagaact ggatttatct gtgtctagtt ttcgattaga tgttctttta tcaaatgttt
    30121 tgaaactatc taggaatcaa gcaaaccagt tgattgaaaa gaaacttgtc caagtaaatt
    30181 atcatgtggt agacaaatca gattacactg ttcaagttgg agacttgatt agtgtgagaa
    30241 aatttggtcg cttgagatta cttcaagata agggacaaac gaaaaaagag aagaaaaaaa
    30301 taaccgtcca gttattatta agtaagtgag gaatagaatg ccaattacat cattagaaat
    30361 aaaggacaag acttttggaa ctcgattcag aggttttgat ccagaagaag tcgatgaatt
    30421 tttagatatt gtggttcgtg attacgaaga tcttgtgcgt gcgaatcatg ataaaaattt
    30481 gcgtattaag agtttagaag agcgtttgtc ttactttgat gaaataaaag attcattgag
    30541 ccagtctgta ttgattgctc aggatacagc tgagagagtg aaacaggcgg cgcatgaacg
    30601 ttcaaacaat atcattcatc aagcagagca agatgcgcaa cgcttgttgg aagaagctaa
    30661 atataaggca aacgagattc ttcgtcaagc aactgataat gctaagaaag tcgctgttga
    30721 aacagaagaa ttgaagaaca agagccgtgt cttccaccaa cgtctcaaat ctacaattga
    30781 gagtcagttg gctattgttg aatcttcaga ttgggaagat attctccgtc caacagctac
    30841 ttatcttcaa actagtgatg aagcctttaa agaagtggtt agcgaagtac ttggagaacc
    30901 gattccagct ccaattgaag aagaaccaat tgatatgaca cgtcagttct ctcaagcaga
    30961 aatggaagaa ttacaagctc gtattgaggt agccgataaa gaattgtctg aatttgaagc
    31021 tcagattaaa caggaagtgg aaactccaac tcctgtagtg agtcctcaag ttgaagaaga
    31081 gcctctgctc atccagttgg cccaatgtat gaagaaccag aagtagctcc agtgcatccg
    31141 acaggtccaa caccagctac agaaactgtt gattcagcac caggatttga agcaccgcaa
    31201 gaatccgtta caattttata agaaacgatg tgagaacaat atcttatcct tatatttcca
    31261 gcgagcagga gatggtgtga gtcctgcatt ccctaacgat aagattatcc tctcaaaaac
    31321 tcaagtctga agctagtaag atttgacgtt tcccacgtta cgggataaga gggagaaaga
    31381 ctaaatcttt ttccgaataa aggtggtacc acgattttcg tcctttttgg aagtcgtggt
    31441 ttttaatttg ttattattta taaaggagat accatgaaac tcaaagatac ccttaatctt
    31501 gggaaaacag ctttcccaat gcgtgcaggc cttccgacca aagagccagt ttggcaaaag
    31561 gaatgggaag atgcaaaact ttaccaacgt cgtcaagaac taaatgaagg gaaaccgcat
    31621 tttgtcttgc atgatggccc tccatacgca aacggaaata tccacgttgg acatgccatg
    31681 aatcatatct caaaagatat catcattcgt tctaagtcta tgtcaggttt taacgcacct
    31741 tatatcccag gttgggatac tcatggtctg ccaatcgagc aagttttggc aaagcaaggt
    31801 gtgaagcgca aagaaatgga cttggttgag tacttgaaac tctgtcgtga gtacgctctt
    31861 tctcaagttt ataaacaacg tgatgatttc aaacgcttgg ggatgtcagg tgactgggaa
    31921 aatttatatg tgactttaac tccagactat gaagcagccc aaatccgtgt ctttggtgag
    31981 atggctaata agggttatat ctaccgtggt gccaagccag tttactggtc atggtcatct
    32041 gagtcagccc ttgccgaagc ggaaattgaa taccatgatt tggtttcgac ttcactttat
    32101 tatgccaata aggtaaaaga tggcaaaggt atcctagata cagatactta tatcgtagtc
    32161 tggacaacaa ctccatttac catcacagct tctcgtggtt tgactgttgg tgcagatatt
    32221 gattacgttt tggttcaacc tgctagtgaa actcgtaagt ttgtcgttgc tgcagaattg
    32281 ttgactagct tgtctgagaa atttggctgg gctgatgttc aagtgttggc aacttaccgt
    32341 ggtcaagaac tcaaccacat cgtaacagaa cacccatggg atacagctgt agatgagttg
    32401 gtaatccttg gtgatcacgt taccactgac tctggtacag gtatcgtcca tacagcccct
    32461 ggttttggtg aggacgacta caatgttggt attgctaatg gtcttgaagt cgcagtgact
    32521 gttgacgaac gcggtatcat gatggcgaat gctggtcctg agtttgaagg tcagttctac
    32581 gacaaggttg taccaactgt tattgaaaaa cttggtaacc tccttctagc ccaagaagaa
    32641 atctctcact catacccatt tgactggcgt actaagaaac caatcatctg gcgtgcagtt
    32701 ccacaatggt ttgcctcagt ttcaaaattc cgccaagaaa tcttggatgc tattgataaa
    32761 gttaaattcc atacagaatg gggtaaagta cgtctatata atatgattcg tgaccgtggt
    32821 gactgggtta tctctcgtca acgtgcttgg ggtgttccac ttccaatctt ctatgcagaa
    32881 gacggtacag ctatcatgac agctgagact attgaacatg tggcgcaact ctttgaagta
    32941 catggttcaa gcatttggtg ggaacgtgat gccaaagacc tcttaccaga aggatttaca
    33001 catccaggtt ctccaaatgg ggagttcaag aaagaaacag atattatgga tgtatggttt
    33061 gactcaggtt catcatggaa tggtgttttg gtaaatcgtc caaatctgac ttatccagct
    33121 gacctttacc ttgaaggttc tgaccaatac cgtggttggt tcaactcatc actcattaca
    33181 tctgttgcca accatggcgt agcaccttac aaacaaatct tgtcacaagg ttttaccctt
    33241 gatggtaaag gtgagaagat gtctaaatca cttggaaata ccattgctcc aagtgatgtt
    33301 gaaaaacaat ttggtgcaga aatcttgcgt ctctgggtaa caagtgttga ctcaagcaat
    33361 gacgtgcgta tctctatgga tattttgagc caagtttctg aaacttaccg taagattcgt
    33421 aacactcttc gtttcttgat tgccaataca tctgacttta acccagctca agatgtagtc
    33481 gcttacgatg aacttcgttc agttgataag tacatgacta ttcgctttaa ccagcttgtc
    33541 aagaccattc gtgatgccta tgcggacttt gaattcttga caatctacaa ggccttggtg
    33601 aactttatca acgttgactt gtcagccttc taccttgatt ttgccaaaga tgttgtttac
    33661 attgaaggtg ccaaatcatt ggaacgccgt caaatgcaga ctgttttcta tgacattctt
    33721 gtcaaaatca ccaaactctt gacaccaatc cttcctcaca ctgcggaaga aatctggtca
    33781 tatcttgagt ttgaagctga agacttcgtt caattgtcag aattgccaga agctcaaact
    33841 tttgctaatc aagaagaagt cttggataca tgggcagcct tcatggactt ccgtggacaa
    33901 gctcaaaaag ccttggaaga agctcgtaat gcaaaagtta tcgggaaatc gcttgaagca
    33961 cacttgacag tttatccaaa tgaagttgtg aaaactctac tcgaagcagt aaacagcaat
    34021 gtggctcaac ttttgatcgt gtcagacttg accatcgcag aagggccagc tccagaagct
    34081 gcccttagct tcgaagatgt agccttcaca gttgaacgcg ctgcaggtca agtatgtgac
    34141 cgttgccgtc gtatcgaccc aacaacagca gaacgcagct accaggcagt catctgtgac
    34201 cactgtgcaa gcatcgtaga agaaaacttt gcggaagcag tcgcagaagg atttgaagag
    34261 aaataaggtt gaaaagtcta ggcaaaattc aatttgagaa gaaaagacaa ctaattttat
    34321 agtctattaa acgcattgta tcacgttttt gaatacctga tatgatgcgt tttttattta
    34381 ttttaaaaat ttgcgaggta tgacttttta tactcaacaa gaatcaaaga gaaacttagc
    34441 aagctaacag tagtaagata aaataggaat ttgatattag ggataagatt gctaaatagt
    34501 gtaatatttt tacaataata aatttatata gttatttttg gtttctgaaa agtattatat
    34561 tttatttcat attatacaaa tttttatttt ataatatcag aaaatacttt ttttaaaagc
    34621 aaatatgata caattttatt tgaaaaaaat aaaaaaggag attttattat gaaattaaaa
    34681 acacttgctt taattagtgg tatcgtcggt cttgtgggag gaattttact tcttattggt
    34741 ccttttgtct tgttgggaac agcggtaaac acagttgcta caactcttaa tggaggagct
    34801 actgcagggg ctttttcagg tgttgcctta ctcttgaatg ccttgaagat tgcaaatctt
    34861 gttcttggta tcattgctat tgtttactat aaaggagata atcgtgtagg tgcagctccg
    34921 tctgtactaa tgattgtttt tggtggagtt agtctcattc cattcttagg atgggttggg
    34981 gggattcttg ttattatcgg aggatctcta ttccttgcaa cattgaagaa attcaaatca
    35041 gaagaataaa aggtatttta gcatgaaaag aacaaaaaag tttatcggta taggagtagc
    35101 tctattatct ctttttcttc tagttgcatg tggaacataa agttcaaagg atacttcaac
    35161 aagtaatgat gagaagacag tagcaacatc caatagttca aaagaaacaa tcactttcga
    35221 tacaccggtt gtaacagacg atgtgattga atcaatacgc acttatgcag attatataga
    35281 tctttataaa aatatttttg atgattattt tactaaagct gaggaaggtt tcaaaggcac
    35341 agctatggaa aataatgact cgtttactaa actaaaagag tcaactcaaa aattattcga
    35401 tgcgcagaaa aaaaggttaa ataatgaaga tagaatagaa acaactaaaa acaatgtgat
    35461 tgccaaacat tgtcaaacag tcctttcctt tttggttttg actagctttt ttgtgaaaaa
    35521 ttgtgtaaaa tagaatagat aaacgagggg aaacctcgga aaatttaaag gagaatccat
    35581 ctaatggtaa aattggtttt tgctcgccac ggtgagtctg aatggaacaa agctaacctt
    35641 ttcactggtt gggctgatgt tgatttgtct gaaaaaggta cacaacaagc gattgacgct
    35701 ggtaaattga tcaaagaagc tggtatcaaa tttgaccaag cttacacttc agtattgaaa
    35761 cgtgctatca aaacaactaa cttggctctt gaagcttctg accaattgtg ggttccagtt
    35821 gaaaaatcat ggcgcttgaa cgaacgtcac tacggtggtt tgactggtaa aaataaagct
    35881 gaagctgctg aacaatttgg tgatgagcaa gttcacatct ggcgtcgttc atacgatgta
    35941 ttgcctccaa atatggaccg tgatgatgag cactcagctc acacagaccg tcgttatgct
    36001 tcacttgacg actcagtaat cccagatgct gaaaacttga aagtgacttt ggaacgtgct
    36061 cttccattct gggaagataa aatcgctcca gctcttaaag atggtaaaaa cgtattcgta
    36121 ggagctcacg gtaactcaat ccgtgccctt gtaaaacaca tcaaaggttt gtcagatgac
    36181 gagatcatgg acgtggaaat ccctaacttc ccaccattgg tattcgaatt cgacgaaaaa
    36241 ttgaacgtcg tttctgaata ctaccttgga aaataaaaaa ttgtaagtct agaattgatt
    36301 tctaggcttt ttatgttagt atggaagtat gataaggaat aaaaaacaag attatgtact
    36361 ggcctacaag caaccagttt caaccactta catgggttgg gaagaagaag ctttaccgat
    36421 aggcaatggt tctttaggag caaaagtatt tggccttata ggggctgaac ggattcaatt
    36481 taatgaaaaa agtctctggt ctggaggtcc acttcctgat agttcagatt atcagggtgg
    36541 aaatcttcag gatcagtatg tttttttagc tgagattcgg caggctttgg agaagagaga
    36601 ttacaatctg gctaaggaac tggctgagca gcacctaatt gggccaaaaa cgagtcaata
    36661 tgggacctat ctgtcttttg gggatattca cattgagttc agccagcaag gtacgacttt
    36721 gtctcaggtg acggactatc agagacagct gaatattagt aaggcacttg tgacgacttc
    36781 ttatgtctat aagggaacgc gatttgaacg tgaagctttt gcgagttttc cagatgatct
    36841 cttggttcag cgttttacta aggaaggggc ggaaactcta gattttacta tagaactatc
    36901 cttgacctgt gatttggctt ctgatggaaa gtatgagcag gaaaaatctg attacaagga
    36961 gtgtaagttg gatattactg attctcatat cttgatgaag ggaagagtta aggataatga
    37021 tctgcggttt gctagttatc tagcttggga aacggatgga gatattagag tttggtcaga
    37081 tagggttcag atatcaggag ccagttatgc caatctcttc ttggccgcta agacggattt
    37141 tgcccaaaat cctgctagca attatcgcaa gaaactagat ttagagcaac aggtgataga
    37201 cttggtggac acagctaaag aaaagggcta tacccaattg aaatcaaggc atatcgagga
    37261 ctaccaagcc ttattccagc gtgttcaatt ggatttggaa gctaatgttg acgcattcac
    37321 tacagatgat ttgttaaaaa attataagcc acaagaaggg caggctttgg aggagctgtt
    37381 cttccagtat ggacggtatt tattgattag ttcgtccaga gactgcccag atgctctacc
    37441 agctaaccta cagggagtct ggaatgcggt cgacaatcct ccttggaatt cggactatca
    37501 cttaaatgtc aatctgcaga tgaattattg gccagcctat gttaccaatc tcctagagac
    37561 ggtctttcca gtcatcaact atgtagatga tttgcgtgtc tatggtcgtc tagcggctgt
    37621 aaagtatgca ggaatcgtct ctcagaaagg tgaggagaat ggttggttgg ttcatactca
    37681 agcgactccc tttggttgga cggcacctgg ttgggattac tattggggtt ggtcaccagc
    37741 tgccaatgcg tggatgatgc aaaccgttta tgaagcctat tcattttata gggaccaaga
    37801 ctatctcagg gagaaaattt atcccatgtt gagggaaacg gttcgttttt ggaatgcctt
    37861 tttacataag gatcagcagg cgcagcgttg ggtgtcttct ccgtcttatt ccccagaaca
    37921 tgggccgatt tcgattggca atacttatga ccaatctctg atttggcagt tatttcatga
    37981 ttttattcag gctgctcagg aattgggact ggatgaggac ttgttgactg aggttaagga
    38041 gaagtctgat ttactaaatc ctttgcaaat cactcaatct ggtcgaatca gggagtggta
    38101 tgaggaggaa gagcagtatt ttcaaaatga gaaagtggag gcccagcatc ggcacgcttc
    38161 ccatctagtg ggactctatc ctggcaatct ctttagctac aagggacaag agtatattga
    38221 agcggtacgt gctagcctca atgatcgtgg agatggcggc acaggctggt ccaaggctaa
    38281 taagatcaat ctctgggcgc gtttgggaga tggcaatcga gcccataaat tattggcaga
    38341 gcagttaaag acatccacct tgccaaatct ttggtgtagc catcctcctt ttcagataga
    38401 tggtaatttt ggtgctacta gtggcatggc agaaatgtta ctccagtctc atgcagctta
    38461 tctggtacct ctagctgccc tacctgatgc ttggtcaaca ggttctgttt caggcttaat
    38521 ggcacgtgga cattttgaag tgagcatgag ctgggaagat aaaaaactct tacagttgac
    38581 cattttatca aggagtggag gagatttgcg agtttcttat ccagatattg agaagagtgt
    38641 gattaaaatg aatcaagaaa aaataaaagc gaaatgcatg gggaaagatt gtatttcggt
    38701 ggcaacagca gaaggtgatc ttgttcaatt ttatttttaa gaagatgtta taaggcagta
    38761 atttgaaact gccttttaat aaggatttaa gaatataagc agttttcaac tagttgaaaa
    38821 aacgttataa tgataatagg aagtaatact caatgaaaat caaagagcac aaactaggaa
    38881 gctagccgca ggttgctcaa aacagtgttt tgaggttgca gatggaagct gacgtggttt
    38941 gaagagagat tttcgaggag tataatttgt ttgatagagg gtgggtctga tggcttatat
    39001 tgagatgaaa cactgttaca agcgttatca ggttggggac acggagattg tggccaattg
    39061 tgatgtgaat tttgagattg aaaaggggga gctggttatt atccttggtg cttcaggtgc
    39121 aggcaagtca acagttctta accttcttgg gggaatggat accaatgatg aaggggaaat
    39181 ctggattgat ggtgttaata ttgcggatta tagttcccac cagcgcacca attaccgtag
    39241 aaatgatgtg gggtttgttt ttcagtttta taatctagtt tctaatctga cagctaagga
    39301 aaatgtggaa ctggcttctg aaattgtgac agatgccttg aatcctgatc aggtcttgac
    39361 agatgtaggt ctggctcatc gtctcaataa ctttccagcc cagctttctg gaggggagca
    39421 acagcgagtc tccattgcac gcgcggtagc caaaaatcct aaaattctcc tttgtgatga
    39481 accgactgga gccttggatt atcagacggg caagcaggtt ttgaaaattc tccaagacat
    39541 gtctcgtcaa aagggagcga cggtgatcat cgtgactcat aatggagctt tggcgcccat
    39601 tgctgatcgc gtgattcaaa tgcacgatgc cagtgtcaag gatgtggtgc tcaaccagca
    39661 tcctcaggat attgacagtt tggagtacta gcatgatcaa gcgaaaaact tattggaagg
    39721 acttaattca gtccttcaca ggctccaagg ggcgtttttt atccatcttg atcctgatga
    39781 tgttgggatc tctagcctta gtaggcctca aagtaaccag tcccaacatg gaggcgacag
    39841 ctaatgctta tttaacaact gctcaaacct tggatttggc agtcatgtct aactatggct
    39901 tggatcaagc agaccaagaa gaactaaaac agacggaggg cgcagaggtc gagtttggct
    39961 atttgacaga tgtgactatg gataatgggc aggatgccat tcggctgtac tccaaaccag
    40021 agcgaatttc aacctttcag ctaagaaagg gacgacttcc tcagtcagac aaggaaatcg
    40081 ctttggccac tcatttgcaa ggccaataca gcgtgggaca ggagattagt tttaaagaaa
    40141 aagaagaggg tcattcctct ttaaaagacc atacttatac cattactggt tttgtggatt
    40201 cggctgaaat cctctcccag cgagatatgg gctacgcagg aagtggaagt gggactctga
    40261 cagcctatgg ggtgatttta cctagtcagt ttgatcagaa agtctacaat atagctcgtt
    40321 tgaaatatca agatttagca ggtttaaatg ccttttcatc agcttatgaa gaaaaatcca
    40381 agcaacatca ggaagacctt gaacaaactt tatcagataa tggcaaggta cgtctgcaac
    40441 ttttgaaaaa agaaggacaa gagtctctag acaaggggca agagaccctt gacaaggctc
    40501 agactaattt gcaggaaggc aagcgtcgtt tagcagctgc tcaagctcgt atacaggctc
    40561 aagaaagtca actagccttg tttcctcaag ttcagagaga gcaggctagt gctcaactta
    40621 cccaagccaa gcaggaattg ggcaaggaag aggacaaact aaagcaagct gaacaaaatc
    40681 tagcccaaga aaaggaaaaa ttagaaaaac atcagcaagt cttggacgat ttggcggagc
    40741 caaggtatca ggtctataat cgtcagacca tgccaggtgg tcagggctat cttatgtata
    40801 gcaatgcttc atccagtatt cgagcagtgg gcaatatctt tcctgtggta ctttatgccg
    40861 tagcatccat ggtgaccttt acgaccatga ctcgctttgt agacgaagag cgaactcatg
    40921 cagggatttt taaggccttg ggttaccgta gtaaggatat tatcaccaag tttctccttt
    40981 atggactagt agctgggact gtcggaacgg ctctaggtag tatacttggt cattatttgc
    41041 tagccagtgt aatttcaagt gtcattacaa aaggcatagt ggtgggagaa acccagattc
    41101 agttctattg gacctatagc ttactagctc ttgtcttgag ttggttggcg agtgtgttac
    41161 cagtctatct ggtggcttgg agggaacttc atgccgaagc agcccagctt ctacttccta
    41221 aacctcctgt caaaggagct aaaatcttat tggagcgtat cggttttatc tggcgtcgtc
    41281 tcagttttac tcataaggta acagcccgca acatctttcg ttataagcag agaatgttga
    41341 tgacaatctt tggtgtggca ggttctgtag ctctgctctt tgcaggtttg ggaatccaat
    41401 cttctgtagc aggagttccg tctaaacagt ttcaacaaat ccaacagtat cagatgcttg
    41461 tctctgaaac tcctagtgcg accaatcagg acaaggtaga gctagcagaa gtgttgaaag
    41521 ggcaggagat actagcctac cagaaaatct attctaaaac gctagacaag gatttcaaag
    41581 gcaaggctgg tcttcaaaac attactctta tgatgataga gaaggaagat ttgactccct
    41641 ttatccatct tcaacatcat cagcaggagc tgacattaaa agatggcatc gttattacag
    41701 ctaaactcgc ccagctggca ggtgtcaagg ttgggcagac tttagaaatt gaaggtaagg
    41761 aactaaaggt cgctgctatt actgagaact acgttggtca ctttatttat atgagtcagg
    41821 ctagctatga gcaactttac ggacagctac cccaagccaa cacttatctg gtctcattaa
    41881 gggataccag tgcaactagt atcgaaagtc aggcgggctt gcttatgaat caatctgcgg
    41941 tgtccagcgt tgtccaaaat gcttcagcca ttcgactctt cgactctatc gctagctcac
    42001 tcaatcagac catgaccatc ttggtcatcg tatcggttct attagctatt gtcatccttt
    42061 acaatctgac caatatcaac gtagctgaga gaatccgtga actctccact atcaaggttc
    42121 tcggttttca taataatgaa gtcaccctct acatttaccg tgagacgatt gtgctgtccc
    42181 ttgtgggaat cgtacttggt ctggtagctg gtttctattt acaccaattt ttgattcaaa
    42241 tgatttcgcc tgcgactatt ctcttttatc cgcaggtagg ctgggaagtc tatgtaatcc
    42301 cagtggcagc agtaagcatc attttgacct tgcttggttt cttcgtcaat tattatctga
    42361 gaaaggttga tatgttagaa gccctgaaat ctgtagagta aggtagttat ttttagctga
    42421 ttgaacttct atttattaat attcaaaaat cctccgtttc aaagagcagg gaactctttg
    42481 tgacagagga ttttttctat agggctttag cagctgcaat tgcggcttcg aagtttggct
    42541 cagaattgat attatccacg tattcaacgt agcgaatcgt attgtcagta tcgaggacaa
    42601 agactgcgcg tgctaatagg tgccattcgt tgatcaagag ggcataatcg cgcccgaaag
    42661 aatggtcaaa gtagtctgaa agcataatgg cattgtcaag gccttcagca ccgcaccaac
    42721 gtttttgagc aaaaggtagg tccattgaaa cagtcaatac gaccgtgttg tccagtccag
    42781 ctaattcttc attaaaacga cgtgtttgag ttgagcagat gcctgtatcg atagaaggaa
    42841 cgacactcag gacttttttc ttgccatcaa aatcagccag agatttttta gaaagatctg
    42901 ttgtagtaag agaaaaatca agcgccttgt cgccgccttg tagttgttta cctgtaaagc
    42961 tcacaggatt tccgagaaaa gttaccatag gatactccaa tcttttttct tccattgtat
    43021 ctgaaacagt cagaattttc caatgatttg accggaaatg tgggcataga aaaaacgcca
    43081 gctcacatga gaatgacgtt tttcagaggc ttattttgcc aatccttcag caatcttgtc
    43141 aaggttgtat ttcatcatgc tgtagtagct gtcgccttct ttaccttgtt ctgcgataga
    43201 gtcagtaaag atttgtgcgt agattgggat gtttgtgtct tgagaaacag ttttcattgg
    43261 acggtcatcc acacttgatt ctacaaagag tgatggaact tttgtttggc gaagtttttc
    43321 aaccaaggtc ttgatttgtt caggagttcc ttcttcttca gtattgattt cccagatgta
    43381 ggcacttggg acaccatagg ctttagagaa gtatttgaat gctccttcgc tggttacaat
    43441 gagtttcttt tcagcaggga tcttattaaa tttatcctta ctttctttat caagtttgtc
    43501 taacttatca gtatattctt tgagattttt ttcatagaat tctttattgt tagggtcttt
    43561 ggcgctcaat tgtttggcga tatttttagc aaaaataata ccgttttcaa ggttaagcca
    43621 agcgtgtggg tcttcttttc ctttttcatt ttgaccttca aggtagataa catcaacgcc
    43681 gtcgctgact gcgaagtagt ctttgttttc agttttcttg gcattttcta ccaattttgt
    43741 aaaccaagca ttgccacctg tttcaaggtt gataccgtta tagaaaatca aatcagcctc
    43801 agaagttttc ttaacgtctt caggaagtgg ttcgtattcg tgtgggtctt gcccaatcgg
    43861 aacgatacta tgaaggtcaa ttttgtcacc agcaatattt ttagtaatat cagcgatgat
    43921 tgagtttgta gcaacaactt ttagtttttg accagaagtt gtatcttttt ttccgctagc
    43981 acatgctaca agaatgattg cagaaagaaa gagaacgagt aatgtaccta attttttcat
    44041 tagatcctcc aatttattag ggctttgccc cttattttaa caaatgttta tttttcagtt
    44101 tcaaatatcg ttgtttggga gcgataaaga agctaatgag aaagaaacta gcagctgtaa
    44161 gcacgatact agaacctgcc gcaacattaa aactatagcc aataaagagt cccaaaactg
    44221 aagcagtagc tccgaaggtt gaggaaagga aaatcatgct tttcagacta ttagcataca
    44281 gataagcagt tgcagctggg gtaatcagca tggctacaat caggatagtt ccgacacttt
    44341 gcatggctgt cacagacacg agagtcagga gtaccatgag aaggtagtga tagaaattga
    44401 caggcattcc catggcttta gccaagagtt catcaaagga agttatcaag agttgcttga
    44461 agaaaatcca gattaacaag aggatggctg cccccacacc catagtaata aacatatccg
    44521 tatcttggac ggccaggata ttaccaaaaa ggatatggaa aaggtcagtt gaacttttag
    44581 cgacaccaat caagatgata ccgagggcta agaaagaaga aaaggtaatg ccgatggcgg
    44641 tatcgctttt aataatcgag tttcctttga tgtaggtaat gatgatggca gctagcaatc
    44701 caaagacaat ggctccgata aagaagtcaa ggcccaagat gaaggagagg gctacacctg
    44761 gtaagacagc atgtgaaatg gcatctccca tgagtgacat cccgcgtaga ataatgaaac
    44821 atcccacagc tccagctaca atcccgacga caatagctgt tatcaaggca ttttgtagga
    44881 aatggaattt ttgcaatcca tcgataaatt ctgcaatcat aggtcacctc cattgaaaaa
    44941 gagttgatta ccgtaagctt cttttagatt ggtttcggta aaagtttctt ttgttggacc
    45001 aaaggcaatc acttctcgat tgacaagtaa gacttgatcg aagtagtggg gaatcttgct
    45061 gaggtcgtgg tgaacgatga gaaccgtctt cccagctttt ttcaaatctc tcagcgtatt
    45121 catgatgatt tcctcactga cagagtcaat cccagcaaag ggttcatcca agaggatata
    45181 gtcggcttcc tgcaccaaac atctggcaat caagacccgc tggaattgac ctccagacag
    45241 ttgactaatt tgacgttcag cgtagtcagc taggccgacg atttcaaggg cctcttgcac
    45301 tttcttccaa tgtttagcct ttaaacttcg aaagagagga atagagggaa atagtcctaa
    45361 cgagacgcat tccttgacct tgatgggaaa gttgtagtcg atattgattt tttgttcgac
    45421 ataggcaatt cggtgtaagg attttttaac ttccttgtca tcgagaaatg cctgaccttg
    45481 atgtgggata attcccagca taccttttaa tagtgttgat ttcccagcgc cgtttggacc
    45541 aatgatgccg gtaattgttg gtccatggag cactagtgaa atatccttaa gtgccaacgt
    45601 ttctttgtag gagacactga ggttttcgat acgtatcata aacttgtatt cctcctgtct
    45661 cttaatatac attaaaaaaa aaattaagtc aagttaattt ttgaaaaaat taaaataata
    45721 actgaaaaat agattctaaa gataactttc aggataaatt tctaaattat aaaacgcata
    45781 gtatcaagtg taaaaaactt ggaattatgc gttttatcat ggaaagattt tttataatag
    45841 ctaaaaaata atagttaaat ggttattaat tgcattccct agtgattttt gttaagataa
    45901 atgcaaatac aaatgaaagc gagaacaaga tgacacgtta tcaagatgat ttttatgatg
    45961 ctatcaatgg aaaatggcaa cagacagctg aaatcccagc agataagtct caaacaggag
    46021 gttttgttga tttagaccag gaaattgaag acctgatgct ggcgacaaca gacaagtggt
    46081 tagcaggtga agaagtgcct gaggatgcta tcttggaaaa ctttgtcaaa taccaccgcc
    46141 tagttcgtga ttttgacaag agagaagctg acggtatcac acctgtctta ccactcctta
    46201 aagaattcca agaattggaa acttttgcgg attttacagc taaactagca gagtttgagc
    46261 ttgcaggaaa accaaacttc cttccttttg gtgtatcgcc agactttatg gatgctagaa
    46321 tcaatgttct atgggctagc gctccaagca caatcttgcc agatacgacc tactatgcag
    46381 aagaacatcc tcagcgcgaa gagctcttga ctctttggaa agaaagcagc gcaaatctcc
    46441 tcaaggctta tgatttctct gatgaagaaa ttgaagactt gctagaaaaa agacttgaat
    46501 tggaccgccg agttgcggca gtggtgctct ctaatgaaga aagttcagaa tatgctaaac
    46561 tctatcatcc atattcttac gaagatttca agaaattcgc gcctgcccta cctttggatg
    46621 acttcttcaa agcagttatt gggcaattac cagacaaggt tattgtagac gaggaacgtt
    46681 tctggcaagc agcagagcaa ttttacagtg aggaagcctg gtctctcctt aaagcaacct
    46741 tgattttgag tgttgtcaat ctttcaacca gctatttaac agaggatatc cgtgttttgt
    46801 ctggtgccta cagccgtgcc ctttctggag ttccagaggc aaaagataag gtcaaagcag
    46861 cttatcatct agcacaggaa cctttcaagc aagccctggg gctttggtac gcccgtgaga
    46921 agttctctcc agaagccaag gcggatgtgg agaaaaaagt ggcaaccatg attgatgtct
    46981 ataaggagcg tctgcttaag aatgactggc tcactccaga aacctgtaaa caggctatcg
    47041 tgaagctcaa tgtgatcaaa ccttatattg gctatccaga agaattgcct gcacgttaca
    47101 aggataaggt agtgaatgaa actgccagtc tttttgagaa tgctctagcc tttgcgcgtg
    47161 tggaaatcaa gcacagttgg agtaagtgga accagcctgt agactataag gaatggggca
    47221 tgcctgctca tatggtcaat gcctactaca atcctcagaa gaacctgatt gtctttccag
    47281 cggccatttt acaggcgcct ttctatgact tgcatcagtc atcttctgct aactacggtg
    47341 gtattggggc agtgattgcc catgaaattt cccacgcctt tgatactaac ggggcttcct
    47401 ttgacgaaaa tggtagcctc aaggattggt ggacagagag cgactatgct gccttcaagg
    47461 agaaaacaca aaaagtcatt gaccaatttg atggacagga ttcttatgga gcaaccatta
    47521 acggtaaatt gactgtatca gaaaacgtgg ctgacttggg aggaatcgca gcagcgcttg
    47581 aagcagctaa gagagaagca gacttctcag cagaagagtt cttctacaac ttcggtcgca
    47641 tctggcgcat gaaaggtcga ccagaattta tgaaactttt ggctagcgtc gatgtgcacg
    47701 caccagccaa actccgtgtc aatgtgcaag taccaaactt cgacgatttc tttacaacct
    47761 atgatgtcaa agaaggagac ggaatgtggc gttcaccaga ggagcgcgtg attatttggt
    47821 aatacaaaaa atctagtcat cagaaaaaag gcatccaatc acgtgtgaaa accttgatag
    47881 gatgcctttt gtaatagaaa gatttgctgg atagtttact taaatttcga ttgtcatcat
    47941 cttgctagaa aaagggattg aaggcctttt cgtgagcgat agtagtagct ggaccatgtc
    48001 ctggatagac atcgtagttt ggtagggtga agagttgggt ctggatacta tgaaggagtt
    48061 gctccatgct accagtcgga aggtcggtcc gtccgatagt ttcgcggaat agagcatctc
    48121 ccgtcaagac taggtgagca tcaggaaaga ctagggaaac accaccgata gagtgccctg
    48181 gggtcggtag aaccttaaaa cgaaattcct ctagttggta ttcttcgtga aagacaaagg
    48241 tgtgttctgc aggttttgtg accacatctg ccatatcatc gtggcgaggg agaccggaga
    48301 gattatcgac aggagtgtag agccagctgg cttcgctctc tgcgatatag acaggaggat
    48361 tgccaaacgt ctcgcgaacc aagtccagac tcatgatatg gtcataatgg gcgtgggtca
    48421 agagaatagc acagatcggt ttgttgatct tctcgattgt ctgacgaatg gcttcccaat
    48481 gactaccagg atcgacgacg atgaggtgct tttcgccttc tagataatag gtattttcat
    48541 aggcaacagg attcacggtt ttatggattt tcatactaac tccaatctca aagaatgtac
    48601 taatactctt cgaaaatctc ttcaaaccgc gtcaacttta tctgcaacct caaagcagtg
    48661 ctttgaacag cctgcggcta gctttctagt ttgctctttg attttcattg agtataaatt
    48721 aagtatagca cagttaggga gaataggtaa ggatttaaaa tgaacttaaa aaccagcatg
    48781 actagatgaa gacaggctgg ttatcaattt atcaatattg gaaaggtgtc aattgccctt
    48841 cataggttgc ataaactccg tcattagctt gtttgataga cgtgatgttt agagtgctac
    48901 cgtagttggc ttctcatttg tcgctatatt cactataagt tatcctactg ggcaggttct
    48961 ggtcgttagc atagtattga acgagttttt tatagctttg ctggctgaat aggaacaggc
    49021 agcaaactcc taacattagg agagcaaaga tagaaatagc agttttttca taggtattct
    49081 ccttataaac taaaggatag aagaaagtat cttgaggatt tttatactct tcaaaaatct
    49141 cttcaaaccg cgtcaacgtc gccttgccgt atatatgtga ctgactttgt cagtcttatc
    49201 tacaacctca aagcagtgct ttgagcaacc tgtggctagt ttcctagttt gctctttgat
    49261 tttcattgag tattaattcc aaaatccagt caaggtacca gtgtatgtga tacgagttcc
    49321 accgtttatc tttcgtatct tcaatggaat tggggaaatc agaaagtctt tgggttacaa
    49381 tatggtttcc gttcttctat ctctccttct atcacctaag tttaaaatct aacttcgtag
    49441 tatattatgg gccaataatt cagaattatc aagcaatttt gaattatttt tttcttattc
    49501 tgttccaatc tagttttcat attcttaaaa aagtgataaa atggtaggaa gaattggaga
    49561 gtagagatgc cgaaagaagt gaatttaaca ggcgaagaag ttgtcgcttt aaccaaagaa
    49621 tatttaacgg aagaggatgt tcattttgtc cataaggcct tggtctatgc tgttgaatgc
    49681 cacagtggtc aatatcgcaa atcaggcgag ccttatatca ttcaccctat ccaagtggca
    49741 ggtattttag ctaagctaaa gctggatgct gtaacagtag cttgtggatt cttgcatgat
    49801 gtggtggaag atacagatgc gaccttggac gatttggaaa gagagtttgg tcctgatgtg
    49861 cgggtgattg ttgacggagt taccaagctt ggcaaggtcg agtacaaatc gatcgaggag
    49921 caattagcgg aaaatcatcg caagatgctc atggccatgt ctgaggacat ccgcgttatt
    49981 ttggtcaaac tgtctgaccg cttgcacaat atgcggaccc tgaaacatct tcgaaaagac
    50041 aagcaggagc gtatttccaa agaaaccatg gaaatctatg ccccacttgc ccatcgtttg
    50101 gggatttcca gtgtcaaatg ggaattagaa gacttgtctt tccgttatct caatccaacg
    50161 gagttttaca agattaccca tatgatgaag gaaaagcgca gggagcgtga ggccttggtg
    50221 gatgaggtag tcacaaaatt agaggagtat acgacagaac gtcacttgaa agggaagatt
    50281 tatggtcgtc ccaagcatat ttactcaatt ttccgcaaaa tgcaggacaa gagaaaacgg
    50341 tttgaggaaa tctatgatct gattgctatt cgttgtattt tagataccca aagtgatgtt
    50401 tatgccatgc ttggttacgt gcatgaattt tggaaaccga tgccaggtcg cttcaaagac
    50461 tatattgcca accgcaaggc caatggttat cagtctatcc atacgactgt ttatggacca
    50521 aaagggccga ttgaattcca gattcgaacc aaggaaatgc acgaggtggc tgagtacggg
    50581 gttgcggctc actgggctta taagaaaggt ataaaggggc aagttaacag caaggaatca
    50641 gctattggaa tgaactggat caaggagatg atggagctcc aagaccaggc tgatgatgct
    50701 aaggaatttg tggactctgt taaggaaaac tatttggctg aggagattta cgtttttacc
    50761 ccagatggag ctgtccgttc ccttcccaaa gattcaggac cgattgattt tgcctacgaa
    50821 atccatacca aggtcggtga aaaagcaact ggtgccaagg tcaatggccg catggttcca
    50881 ctgacaacca agttaaagac aggggatcag gttgaaatta tcgccaaccc gaactccttt
    50941 ggacctagcc gtgactggct caatatggtc aagactagca aggcgcgcaa taagattcgc
    51001 cagttcttta aaaaccaaga taaggaattg tctgtcaaca agggtcgtga gatgctgatg
    51061 gctcagttcc aagaaaatgg ctatgtggca aataaattta tggacaagcg ccacatggat
    51121 caagttctgc aaaagaccag ttacaagaca gaagactccc tctttgcggc cattggtttt
    51181 ggggaaatcg gtgcgattac cgtctttaac cgtctgactg aaaaggaacg ccgtgaggaa
    51241 gagcgtgcca aggccaaggc tgaggcagag gagcttgtca aaggtggcga ggtcaaggtt
    51301 gaaaataaag aaactctcaa ggtcaagcat gaggggggag tggttattga aggtgcttct
    51361 ggtctcctag tgcggattgc taagtgttgt aaccccgtgc ctggtgacga tattgttggc
    51421 tacattacca agggtcgtgg tgtggctatt caccgtgtgg actgtatgaa cctgcgtgcc
    51481 caagaaaact acgagcaacg tctccttgat gtggaatggg aagaccagta ctctagctca
    51541 aataaggagt atatggccca tatcgatatc tacggtctca accgtacagg actgttgaac
    51601 gatgtactgc aagttctttc aaatacaacc aagaatattt caacggtcaa tgcccaacca
    51661 accaaggata tgaagtttgc taatatccat gtgtccttcg gtattgccaa cctctctaca
    51721 ctgaccacgg ttgtcgataa aattaagagt gtgccagaag tttactctgt caaacggacc
    51781 aacggctagt tgtcctagct cttactagaa aggctattat gaaaatcatt atccaacggg
    51841 ttaaaaaagc ccaagtgagt atagaaggcc agattcaggg aaaaatcaat cagggacttt
    51901 tattgctggt tggtgttgga ccagaggacc aagaggaaga tttggactat gctgtgagaa
    51961 aactggtcaa tatgcggatt ttttcagacg cagaaggcaa gatgaacctg tctgtcaaag
    52021 atattgaagg agaaatcctc tccatttctc agtttaccct ctttgcggat actaagaaag
    52081 gcaatcgtcc agcctttaca ggggcagcta aacctgatat ggcatcagac ttctatgatg
    52141 ctttcaatca aaaattagcg caagaagtgc ccgttcagac aggtatcttt ggagcagata
    52201 tgcaggttga gctggttaat aacggacctg ttaccattat cctagatact aaaaagagat
    52261 aagaaagacc aagcccagtc ggcttggtct ttctcatcga tcataaaata ctccaaaaag
    52321 aaatcggttc ttgatatgct tgggggaagt ctcttttcag gctttggcag atgcgatagg
    52381 aagggatgag atgtcctagg atgaggagag ttccctgaag gaaaagtgga atatcactta
    52441 aagacaccaa ggagagaatc atcaggctat cccataggaa aatttgtaaa gcaaaacgcc
    52501 agaatctcgg tctaatctgg gaatagagtt gactaaagtg gtcacaaagt tggtttgaaa
    52561 gataggtcaa tagcacggga tgaaagaaaa tgagccaacc actataaatc aaaatccagt
    52621 cccaagtaag cccccctttt aaatgtcaaa aataccagca tgattccatc aatgacaagg
    52681 agttgaatgg ttttgatgaa gatgattttt ttcatgctaa aacctccttt tccttagaga
    52741 cactccccta caggtatttt agaatgtata ttgcttagat attccttatt ttattagctt
    52801 tttaaggtat taaagtagtt atataagttt atgaagggta tcctatcctg cacggctaat
    52861 tttgagttcg aaactgagga gtttataact caaaatttcg attaaatcag catttaacga
    52921 tagtatgttt gtcataaaat gataaatttt tagattgtgg aatttttttc aacttaattc
    52981 ttgatttttt ttctaaattg ttatatactt aatttacggg ataacagaat cccgtcagaa
    53041 aggggtgata atatgggaaa agagaatgtt agaatgcgtt tagcgtctga atccagacag
    53101 atattagata agcttaaaga tgatgtttac aataaattag gctatgaagt aagctattcc
    53161 agtattgtta gtcaagctgt tagagaatac gttcctaaaa aagaacgtat agattggata
    53221 aaattaaaag agacagctat acctttttca tcgttaaaac agagcaataa ttgggagtac
    53281 cagacatcac ttatgttaga gaaagatgta ttaatcttac tgtctgaact acaaaatttt
    53341 ttcttagatg tcttccaagc gaaacgtata catagggcat tttgtatacg tctatgtttg
    53401 agagcgcaat ttttattaag taataatgat tcttaatact atcaatatta atgcggagtg
    53461 taatgctccg caaaaataaa acctattacg ggataaaaac actactttat ttaggatgga
    53521 aataagaacg tattttttac taatattgtt tagaaaggag tttctttatg acaccagaag
    53581 aacaaactgc tttggaatac ttttatgagg aactcgagag ttacggtgaa gatgagcaag
    53641 gagaggtctt tgatttgtat gataattaaa tcatctccat agtttctgag aataattgag
    53701 aaactatact ctaaatcata ggtttatgta aagctagata tacacaaaag gaggtacgaa
    53761 tgaagatgaa agctagagct tcaccgttga attgattttg tttctagaaa aaatcaataa
    53821 ttagaaaaat acgaggtaaa aaaatgaata gaaatgtact aaattttctg cgaacagaaa
    53881 gtgctgaaag agttagtctt tatatagaca aggctaatag attggagggc gatgtaactc
    53941 tactcgcacc tagttctcaa gatttggaag atattaaaaa tgctatgttt tccaatccta
    54001 atctcgagct aaaagtagcg agactggatg tgatgaagaa aatagcatat gcttctacaa
    54061 gaaatcatta tttaacaggg gctacgattt ttggggatat ttcgaaagga acatacaatt
    54121 gtgatcctaa aagttatgtc taatataagg gaggttaatc cctccccttg tttttgaaaa
    54181 tcgaaaggaa aagtataaaa atgaaagtta atgaatattt ctggagaacg agctgtctta
    54241 tactaaaaaa gaagaaatcg ataaaatagt atacgtgtta gagatgtgtt tgtcgtaaaa
    54301 gtctggatag ttttctctta ggtagtcagc tgtcatataa taaaaagtag aatggcaagc
    54361 agtgatgatt ggcataagag atgaaaaacg ctgagaacta atgaaggcta agagaacaaa
    54421 aaatagaaaa tcaatggtga gaacccctaa atagtagaag cgaatctttt tattgatttg
    54481 aggataaatc tcagcgaatt gatgtttgag tcggacgacc aagagaaata gcaggagtcc
    54541 ttgagcaagg aagaaaatta aaccagagag caagagccac tcaaaagatg tctcgagtct
    54601 agtgataaaa aatgcaaata gtagcaaatc aataactgca attgctataa aatggattct
    54661 atagagtttt ttcatgacaa gacctccctc ttttatctaa cttcattcta ctctaaaaga
    54721 atgggagtta caactaaaat gataaaaata gcagagggga gattctctta agttggctag
    54781 tattctttat ttgagtttcc ttctattatc taacttcttc atcattccag acaaataaag
    54841 ctccgattgc attgaggata taaaagatgt atttaccgat attggcgaag tttccttgaa
    54901 taccagcttt tgtcagctga acgaaattgt aaatcaacca aaagccccac tgagttgtta
    54961 gttttaatgc attcaaagca ttggcaatga gggacagtgc aaaggcaata gttgttacgt
    55021 aggcaaggag attcatcttg cccccatatc cgatatagtt ggtcacaaag gcaaagagga
    55081 aggcgatgat ggaaatgatg atggccgcca attttacctg tttttggctc atttggttgg
    55141 gtctgccttc ttgcgaagct ttccatttct ttatagcaaa ggtataaatg aggaaggtga
    55201 cgggataggt aatgatggcc gccttatttc caaggatata atcaatagca ccggacaaaa
    55261 tggtattaac aataccaaag taattttccc atttgcttaa tttccccgtg aaacgagtgg
    55321 acaacatgga aatcccaacg ttggttacgg aaatcaatcc aaagggtaca agagctgtcc
    55381 atggtcccca gtctacaaat ttatcgaggc gtgagttgag gtaaccagat gcaatcgcaa
    55441 tccaacgacc aaagcaaccc cgaagaggtc aaactattta gatgtagcaa aaatttttag
    55501 tgattttttc ataggttaaa ctacctttct ttttttcaaa tattctccca ttttttttca
    55561 aatattctac caccaaatga aagtaaaata aaatgataga aataaaaccc tgaaaataaa
    55621 actttcaggg ttggtagggg acttgagaaa ttagttgatt ttctcgacat agagttgttt
    55681 tgcaatgtcc atattcactt gtaaatcctc gtcgttactg aggatagtga aggtattgct
    55741 gaagccatca aactgcttaa cttggagctg gtcaccgatg tgaagtgagt gcttgtccag
    55801 ataatggaga atgtcaaaac tatcgtgcat ccgagtcagg cggtaggcgc cagcttcctt
    55861 gatatcagct agtgggaggt tattgatttc aacgaggagt tctcccttgg caggaatagt
    55921 tcctccgtgg gggcaggttt tagggaaacc tagcagttta tctagtcttt ccacgaacag
    55981 gtcagagaca gtgtgttcca agatctcagc ttcctcgtga atctggtcac ttgtatagtc
    56041 taaatgatga actagaaaaa cttcaatcaa gcggtgctta cgatagagct cagagaccag
    56101 tttgagaccg aggtcagtca gtagatagcc acattccttg tcctttagga tgagattttc
    56161 acttttcatt cgtttgatca tttcagttac ggcaggggga gagacttgca tgcgagccgc
    56221 aatttccttg ttggtaatct tatgcaggtc tatgccaatt tcataaatac attttagata
    56281 gtcttctttg tttggggtca ttcgtttcct cttgtctaat atctttatct agtatatcaa
    56341 aaaaagctag atttctctag ctgtgacttt ttatgttata cttattgcaa gagaaaaaac
    56401 gaggagatgg atatgacgaa aatagctctt ctttcagata ttcatggaaa taccaccgcc
    56461 ttggaggctg ttttggcaga tgctcggcag ctaggagtgg atgaatactg gcttttggga
    56521 gatattctca tgccagggac agggcgtaga aggattttgg acttgttgga tcaactaccg
    56581 attacggcta gagttttggg aaactgggaa gacagtcttt ggcatggtgt ccgtaaggaa
    56641 ttggatagta ctcgccctag tcaacgctat ctcttgcgcc agtgccagta tgttttagag
    56701 gaaatttccc tagaagaaat tgaagtgctc cacaatcaac ctcttcaaat tcatcgtcag
    56761 tttggggatt tgacagtggg aattagccat catctgcctg ataagaactg ggggcgagag
    56821 ttgattcata ctggcaaaca agaggaattt gaccgcttgg tgactcatcc tccctgtgat
    56881 attgctgttt atggacacat tcaccagcag ttgcttcgtt acgggactgg tgggcaattg
    56941 attgtcaatc cgggttcgat tggccaacct ttctttttgg acgctcagct acggaaggac
    57001 ttgcgggccc agtatatgat tttggagttt gatgacaagg gcctggtaga tatggacttc
    57061 cgacgggtag attacgatgt ggcagctgaa ttgcagctgg ctaaagacct gagacttccc
    57121 tattttgagg tttactatga aagtctggtc aatgggatcc accatactca tcatcaggaa
    57181 tttttgagag aattggctca gaaagagggt tgcgatcggg agttagacga ctggttgaaa
    57241 agtggtaacg attgacatta caactaaaaa gggtataata atagaaaaag aagagtagag
    57301 gcagggtagc ctcgtaaaaa ggagaagagg atgcaaattc caagtagatt taccattgcg
    57361 actcatatgc tgataatcat tgccctcgag gggaagaaaa gcaaggtgac cagtgatttt
    57421 ctggctgcta gtgtcggggt caatcctgtc attatcagaa agatcttgtc ccagttgaag
    57481 aaggcagagc tgatttcagt agcgcgtgga acgggcggaa cagatattgt caaggacctt
    57541 aaggatatta gtcttttaga tgtttatcag gcggtcgaat gtcttggtaa gacaggtcaa
    57601 ctcttcagtt tccatgacaa tccgaatcca aattgccctg taggagctca tattcatgat
    57661 gttttggatc aaaaattgga gagaattcag ttgactatgg aggcagaact tggtcaaacc
    57721 agtctagaaa aagtcgtggc cgatgcagag agtcagatga aggattaaga gggataaatg
    57781 ccctcttttt tctttggata attatgataa aatgtagcta ttaaaggagt aagaaatgta
    57841 tctcgttata ggaattgtat tagcctttat agtgtcattt tggaaggata atagaagttt
    57901 gtggaatcca gtattatttt tattatccct catttcaagt tatttttatc tatcctacct
    57961 tttttataaa aatggatacg agaatgttca gttagctttt tatgtatttg cctttgtctt
    58021 acttcctttt ttgctttttc taagtggcat ctttttaatc tataatgggg tcattttgtt
    58081 gaagcgagag ggacgttcca agccccatta tctctcaatg ctctttgatt tttattgact
    58141 attagattct atttaaaaag acttagaatg cacatgaaag tatgtattct aggtcttttg
    58201 ttttgtatac atttaatttt caagagtatt atctattttt ccatgcctgg taacgagtat
    58261 caatcaataa ctgggtaagg cgacccaagg tttctctttc ttctggagtg agggattgag
    58321 cttggacaaa gagatgatga atgtattcaa gcgcctttaa ggaagccaag tcattgatag
    58381 agctaatacc agcctcaaga atagtgccaa agaagaggtc gaagtagagc tgaagttctt
    58441 cgtcagggac tgtggccacc cattctttaa aggttgtgtc tacttgctgg ctatcactgt
    58501 tggtcttatc cagttggacg aagtgcttgt cctcaatctg ccaactaaag gtatcgtgct
    58561 gggcgatgcc acccagggca gtactctgaa cgatgatttg gtgagcagga atttccagca
    58621 tcataccgat aatggaacct tgtggaatga atatcttgct tctatccatt atcctttgat
    58681 aacccgcagt ctgtgtcaat tcttgatgga gaccaggtgc atcaaatgta taaactgctg
    58741 tgatctgatt ttgcaaactt tgctcaattt ggctagcagc atagatagcg agatttcctc
    58801 ccttggaatg cccagctaga ataacctttt gcttaggatg atgggcaaaa aagttcttta
    58861 aatagcgaag ggcgtgcttt tgagcaggaa tttccttcat ataggtcagg tggaaatctt
    58921 ccttccagcc aatgatactg tcatctgtcc cacgaaagac aatcagatag gtatcgaggc
    58981 tgacacgata agtcatagcc gcaaattgct tttgcagttc agggtcgatg tcgttgataa
    59041 aatgggagag tttgcaattt ttgaagcgct tgtgttgagc caatttatct aataattgaa
    59101 ggcgattttt gctagtaagc atggtgggct ctcttggaac ctgaggtgct agatctaaaa
    59161 gacgttgagg aagtgtggag accagattat caaaggagag gtaggtgatt tctattaagg
    59221 ttagaatgtc taactcattc aagggaaggt cgtaataaga atcatatgcg acatctttca
    59281 gatagtcaaa aatattggcc ataagagctc ctttcttttt atttatctta gcaaatttcc
    59341 ctcaaattag ctagtagcat agcctgtttg cactggctaa aaacaggcta tttcaaattc
    59401 agtttcagac catctagcat ggaaaaatct gttataataa tggaaaagga gaagcgcatg
    59461 cacaagattt tattaataga agatgatcag gtcattcgtc aacagattgg gaaaatgctc
    59521 tctgaatggg gatttgaagt ggtcctggta gaagacttta tggaagtttt gagtctattt
    59581 gttcagtcgg aacctcatct ggtcctcatg gatattggtt tgcccttgtt taatggttat
    59641 cactggtgtc aggaaatccg caagatttcc aaggtaccta tcatgtttct ttcttcgaga
    59701 gaccaggcta tggatattgt catggcaatc aatatggggg cggatgactt tgtgaccaag
    59761 ccttttgacc agcaggttct tttagctaag gttcagggct tgttgcgtcg ttcctatgag
    59821 tttgggcgtg atgagagttt gctggaatat gctggtgtta tcctcaatac caaatccatg
    59881 gatttacatt atcaagggca agtcttgaat ttgaccaaga atgaattcca gattttacgc
    59941 gtgttatttg agcatgcagg caacatcgta gcacgtgacg acctgatgcg ggaactttgg
    60001 aacagtgact ttttcattga tgataatacc ctctctgtca atgtggctcg tttgcgtaaa
    60061 aagttggagg agcagggatt ggtaggattt atcgagacca agaaaggaat agggtacgga
    60121 ttgaagcatg cttgattgga aacaattttt tctagcctat ctgcgctccc gtagtcgtct
    60181 ttttatctat ctgctttctt tggcatttct tgtcttactc tttcagtttt tatttgccag
    60241 tctaggaatt tacttcctct actttttctt cttgtgttgc tttgtaacca tcttattttt
    60301 cacttgggac atattggtgg aaatgcaggt ctatcgccag gaacttctct atggagagag
    60361 ggaagccaag tctcctttgg aaatagcttt agcagaaaaa ttagaagcgc gtgagatgga
    60421 actctatcag cagaggtcaa aagcagaaag aaaactgacg gatttgctgg attactatac
    60481 cttgtgggtc catcagataa agacccccat tgcagccagt caactcttag ttgcagaagt
    60541 ggtcgaccgc caactgaagc agcagctaga acaggaaatt ttcaaaatcg actcttatac
    60601 caacctagtt ttacagtacc tgcgtttaga aagtttccat gatgatttgg tcttaaagca
    60661 ggttcaaatt gaggacttgg tcaaggaaat aattcgtaaa tatgctcttt tctttattca
    60721 aaaaggctta aatgtcaatc tacatgacct tgataaagaa atcgtgacgg ataaaaagtg
    60781 gctgctagtg gttattgagc aaatcatctc aaacagtctc aagtacacca aggaaggtgg
    60841 tctggagatt tatatggatg accaagagct ttgtatcaaa gatacgggaa tcgggataaa
    60901 aaacagtgat gtcctccgag tatttgaacg tggcttttca ggatacaatg gccgtttgac
    60961 ccagcagtcc tctggacttg gcctttatct atctaagaaa atttctgaag aactggggca
    61021 ccagattcgt atcgagtctg aggtcggaaa aggaacgaca gtgcggattc agtttgctca
    61081 agtgaactta gtccttgagt aaaaatagat gagaaaaagc ctccagattg gaggctttta
    61141 tgctagactc ttatttctca acgcgtgatt tgttggcgat atcagctagg atagcttgaa
    61201 caaaattatc aactgagaca gtttgtgttt ctttttggcc gtagcgacga acgttgactg
    61261 tttcgtcttc catttctttg tctccaacaa ttaattggta aggaatcttg ctggtttgtg
    61321 aagcacggat cttgaactgc attttttcat tgcgctcatc tacgtctgca cggacaccgc
    61381 ggtcacggag tttcttggct acttcccaag cgtagtccac gtgtttttcg ttagatactg
    61441 ggataagggt tacttggtgt ggtgccagcc atgttgggaa ggcacccttg tagttctcaa
    61501 tcaagatagc tgtgaagcgt tccatagttg agataacccc acggtggatc atgactggac
    61561 ggtgatcttc gccatcagct ccgatgtatt tgaggtcgaa gcgttctggc aacaagaaat
    61621 caagttggat agtagaaagg gtttcttctt ttccaagggc agttttaatc tggatatcca
    61681 attttggtcc gtagaaggcc gcttcacctt cggcttcaaa gtagtccacg cccatttcat
    61741 caagagctgc acgaagcatg gtttgggcat tttcccacat ctcatcgtta tcaaagtact
    61801 tatgagtatc ttgagggtca cgaagagaga ggcggaagcg gtagtcagtc aagttgaagt
    61861 cttcataaac atcgataatc aactgaaggg cacgttggaa ttcttcttgg atttgttctg
    61921 gagtaacgaa taggtgaccg tcgttgagtg acatttcacg tacacgttga aggccagtga
    61981 gggcaccaga tttttcgtaa cggtgcatca taccgatttc agcgatacgg attggcaatt
    62041 cacggtaaga gtgaacatgg tgtttgaaaa cttggatgtg gtgcggacag ttcattggac
    62101 gaaggacaaa ttcttccccg tcacccatgt ccatggttgg gaacatgtct tcttggtaat
    62161 gatcccagtg gccagaagtc ttgtaaagct caacagaagc aagtggtgga gtgtagacgt
    62221 gttggtagcc agaagccaac tctttgttta cgatgtagcg ttccaattca cgacggatag
    62281 tcgcaccatt tggcaaccag aatggcaaac cttgtcccac ttcttgtgaa atcataaaga
    62341 ggtcaagctc tttaccaagt ttacggtggt cacgttcctt agcttcttca cgcatttgaa
    62401 ggtagttttt caagtctttc ttttcaaacc aagctgtacc gtagatacgt tgcatcatag
    62461 cgttgtcgct gtttccacgc cagtacgcac cagctacatg gagaaggtgg aagatttgga
    62521 tacgacctgt tgatggaacg tgaggtccac ggcagaggtc tacatattca ccctgacgat
    62581 agatagtcaa accgccttcg tcttctgagt gttcttcaat caattccaac ttgtaagggt
    62641 catttttgaa gatttcacgt gcctcgtctt tagtcacttc ttcacgaata gatgggaagt
    62701 tttctttgac gattttttgc atttcttctt cgatacgagg aaggtcttcg ttagagattt
    62761 gaccagctgt gttgtcagta tcgtagtaga aaccatcttc gatggctgga ccaactccca
    62821 agtgaatgtc tgggaaaaga cgacgagctg cttgggcgaa caagtgagct gctgagtggc
    62881 gcaagattgg aagggcatct tcgtgatcag gtgtcacaat ttcgatgctt ccatcttcag
    62941 tgatagcgcg agtagtgtcg atgagtttgc cgttgaattt accagccaag gcttttttag
    63001 ctagggaatt gctgatagat tgggcaattt caaaagttgt tacgccagat tcgaattcac
    63061 gaacagcgcc atctgggaaa gtaatgttaa tcatgatgtt ctcctttttt attatttcat
    63121 agtttctaag cattgatttt ttatcatcag actacttcca agtcctaacg ttcaattgct
    63181 tttctttatc agagaggaag ttttccattt ccgtcagaat gtctatatcc aaacgttgag
    63241 aaagttctaa tagccaccag atattttctg aaagtttttg ttccagtgtg tagggtgttt
    63301 catcatagta gcgtccttgc tttgtcatca ccagtcgttg gaaatttcca atatcattag
    63361 ataaagccaa ggggtcttct tctaccgtcc acttggaatc atgatgctta acttccagtt
    63421 cgtgataagc ttgtcggatt gcccaactgc gctccactaa ttcttgcaac tccataatta
    63481 gtatcctaat aaaaaaatgc gacatcctcg aaaggacgtc gcaacgtggt tccaccttca
    63541 tttatgtacc tcaaaagaga gggacacctc tgattggctc taacgtggcc accgttttat
    63601 gttttcataa aaaactcaag agtagtatca gtttagactt cctatgcgtt tccagcaacc
    63661 acgcactctc tagtagaaag ggactaagtg acttgtctct atggattatt atagcatgat
    63721 tgtgagattt ttcaaggatt ttgtgaaagt tttttgtttt tcattttttg tagaatatgc
    63781 ttgatgctaa taccagaatc catgcccacc agagcaactg tttcatagat tagcaagtaa
    63841 attagtataa actcaccaaa ggttatgata gtaaagtaaa agagcagtcc gttaccaact
    63901 aaccatcaaa gtgagaaacg gaaacggtag ctatagagta acgatacaat caagattacc
    63961 agaggtgtaa agaagagaag attgtttata tagagttcct tgagtgtaag ggggtcagaa
    64021 acaactagta agaacaactc ataaagtggc caataaaaga ataatatgac cagatagtag
    64081 gggagatggg aatagaatct acgcatgata atcacctcgt tttaaaaaaa cagacaactt
    64141 agatctcttg ctatgattat tatagcatgt ttaaaacaag aaagtaaatt tgttggcaaa
    64201 gaagattttt ttttcactag aatcatggtt agttttcttg gtagaaatat tttgataata
    64261 attaagcaaa actattgaca ttgattatta agagtgtcac aattgttgta actaaaataa
    64321 ttacaataag agaggtataa aaaatgaaac tagcagttat tgcagcaaat ggacaagcgg
    64381 ggaaagcaat tgttgaagaa gtagtcaaac gaggacatga agtaacggct attgttcgtt
    64441 ctgaaaacaa aagtcaatct gagtctatta ttaaaaaaga tttatttgag ttgacaaaag
    64501 atgatcttac tggatttgat gctgttattt cagcgtttgg tgcttataca ccagatacgc
    64561 taccgctcca cagcaaatca atcgaactat ttaatcagct tcttgctgga acacagacac
    64621 gttttctagt tgtaggggga gcaggaagtc tttatataga tgaaacaaaa acgacacgac
    64681 ttttagatac gtctgatttt cctgaagaat ttaagtcact agcaaaagct caagcggatg
    64741 aattggatct tcttcgtaca aaaaataatc tcaactggac ttttgtcagt cctgctgtag
    64801 attttattcc cgatggtgaa aaaacaggaa actacattct agcaggagaa atttttacta
    64861 ctaatgaaaa aggaataagc cagattagtt atgcagatta tgctattgga ttggtagatg
    64921 aacttgaaaa aggtcatcat ataaaagaac gtatctcttt acttgaaaaa taataactat
    64981 gagagtatta atgtttgaca gaaaatttaa atcttggtaa aatagggatt gtcgtaaaga
    65041 caaataactt cttcttggtt acaggcatgc caacctgtca ctcggatgaa gccaaataaa
    65101 aaggagaaac atcatggcaa tctcaaaaga gaaaaaaaat gaaatcattg cacaatatgc
    65161 acgtcacgaa ggtgatacag gttcagtaga ggttcaagtt gctgtcctta cttgggaaat
    65221 caaccacctt aacgaacaca tcaaacaaca caaaaaagac cacgctactt accgtggatt
    65281 gatgaaaaaa atcggtcgcc gtcgtaactt gcttgcatac ttgcgtaaaa acgacgttaa
    65341 ccgttaccgt gagttgatca actctctagg acttcgtcgc taattcaaga tacaaaggcc
    65401 gtcaaaaaca caaagcaaaa ataggaaaat tgacgaagaa acttcagttt ctaggagatt
    65461 ttatcttttt tgccaagtgc ttaggccgtg ttcaattgag catatcttga taataaagct
    65521 actctaaatt gggtagcttt gttgcgttgt caccttacct cgctatactc aagtataatc
    65581 ttcggttacg gttcctagca ctgtaaggta aaataaacta gcttttctcc cttcggggag
    65641 atttttttgt ttcactgaaa gtttcgtacg atcttcacct cgtcccctag tctataaacg
    65701 atttatctag caagaatcat gatgctaagg gcgtcaaaaa tccgtatgaa aatagggaaa
    65761 tgacacagtg ttcgatgagc acaaggagtt tcatcttttt cactaggatt ttagcccggg
    65821 ctaaaatcag ctctctgact tcagagggct tttttattga ggttttatcg gtaacaattt
    65881 tggagactac aaaaacctca aatggtatca gctaattaca ccataaaggt gcgcagttac
    65941 tcggcttttc aagccgagta gctgtatgca agccccctag aagagcccac tctttacaaa
    66001 gtaaagtata gtatgttata ctttacatgg aagtagtcac tgaattccag ttagaaatta
    66061 ctttgtaact acgttttgag gaggagtaaa atgctttcct acgttcgaca ttacccacta
    66121 gcgatagcta aattaatgtg tctgtgctct cctaaaatct gctgatttat tactgactaa
    66181 tacaggaggt tttttttatg ggacagacaa tcatatctgc tattggtgtt tatatttcca
    66241 ccagtatcga ttatttaatt attttaatta ttttatttgc acagctatca cagaataaac
    66301 agaaatggca tatttatgcg gggcaatatc taggcacagg cttacttgta ggggcgagtt
    66361 tagttgctgc ttatgtcgtt aatttcgtgc ctgaagaatg gatggttgga ttgcttggtt
    66421 taatccctat ctatttaggg attcgctttg caattgttgg agaagatgcg gaagaagaag
    66481 aggaagaaat tattgaaaga ttagaacaaa gcaaggcaaa tcaactgttt tggacagtta
    66541 cattgctgac aattgcgtct ggcggagata atttaggtat ctatatacct tattttgctt
    66601 cgttagattg gtcacagacc ctcgtggcct tgcttgtgtt tgtaatcggc ataattatct
    66661 tttgcgagat tagtcgggtg ttatcctcta ttccgttaat attcgagaca attgaaaaat
    66721 acgagcgaat cattgtgccc ttagtattca ttctacttgg actatacatc atgtatgaaa
    66781 atggcacgat agagactttt ctgatcgtgt agattttttt gtttcactag gattttagcc
    66841 cgagctcaaa tcagctctct gattttcagg gggctttttc attgagtata aatggacaat
    66901 cttaaagtta ctctattgta gagtggcttt tgatttgatt aggagattct tataaatata
    66961 gtaaaatgaa ataagaatag gacaaatcga tcaggacagt caaatcgatt tctaaaaatg
    67021 ttttagaagt agaggtgtac tattctagtt ccaatctact atataaatga aaaccttagc
    67081 tgtttggcca aggttcattt cttctatgca agttcttctc ttttcttatc tgggttccag
    67141 atgaagcttg cgataaagct aaagatgatg gttaggatag cgaggataat agcaaggatg
    67201 agggcaggga tgcggataaa ccaccagagt ggatttggtt ttttatcatt gtgccattgt
    67261 ttcgtttctt cgtccagacg ttggaattct gtttgaatac gattggcgac tgtttcaatg
    67321 ccttcatcat tcattttctt gatatctgag atatcgattg gatttccaaa gttcatatcg
    67381 acacgttcac ggctaatcaa gcccttcaaa gtcatgggac cggtgtaggt aaccggcatg
    67441 atacggacct tggccatttt ggcaatcagt gctgcgcccc ccttgacatc gtttgagtgg
    67501 cggctaccac ttggaaacat gatgagagag cggtcacttt ttttgagaac gttgatagga
    67561 tatttgatgg ctgaggcgct gggattttca cggtcgatgg gaaaggcgcc acacatacga
    67621 atccaccaac caaagatacg gttggtaaag agttcttttt ttgccataaa gatgaactgt
    67681 tttggcttgg tcgcaaaggc catataaaca ggatcccacc aggtacggtg aggcgcaact
    67741 aaaatataat tttcatcttg attaggaatt ttatcagtat tatgatagtg agcattgcca
    67801 ttgatggacc ataagagcaa tacaactaat ccacgcaaat aagtataaaa catgcgatct
    67861 ccttcgattg ttttcttgtt attattatac cttatcaaag gagggctggc aaacttttcc
    67921 cttgactaga tacatattta ggatgaaatt agaattctgt taaaaaaaat gatataatag
    67981 aatttatgga taaaaataag attatgggat taacccaaag agaagtcaag gaaagacagg
    68041 ctgagggttt ggtcaatgac tttaccgcat cagccagtac cagcacttgg caaatcgtta
    68101 aacgaaatgt ctttaccctt tttaacgctt tgaactttgc cattgctttg gctcttgcct
    68161 ttgtgcaggc ttggagcaat ctggtcttct ttgctgttat ctgctttaac gctttttctg
    68221 ggattgtgac cgagctacga gccaaacaca tggtggacaa gctcaatctc atgaccaagg
    68281 aaaaggtcaa aaccatccgt gatggtcagg aagttgctct taatcctgaa gaattagtgc
    68341 taggagatgt cattcgtttg tctgcaggag agcagattcc tagtgatgcc ttggttttgg
    68401 aaggctttgc ggaagtcaat gaagccatgt taacgggaga aagtgatttg gtgcaaaagg
    68461 aagttgacgg cttacttttg tcaggaagtt tcctagccag tgggtcagtt ttatctcaag
    68521 ttcaccatgt cggtgcagac aactatgctg ccaaactcat gcttgaggct aagaccgtta
    68581 aacccatcaa ctcccgtatc atgaaatcgc tggacaagtt ggctggtttt actgggaaga
    68641 ttatcattcc ctttggtctg gctctcttgc tggaagcctt gcttttaaaa ggcctgcctc
    68701 tcaagtcatc cgttgtaaac tcgtcgacag ctcttttggg aatgttgcct aagggaattg
    68761 cccttttgac cattacttcg ctcttgactg cagtgattaa gttgggcttg aaaaaggtct
    68821 tggtgcagga gatgtactct gttgagacct tggcgcgcgt ggatatgctc tgtctggaca
    68881 agacgggtac catcacccaa ggaaagatgc aggtggaggc tgttcttccg ttgatggaaa
    68941 cgtatggtga agaggctatt gccagcatct tgactagcta catggcccat agtgaggata
    69001 agaatccaac tgcccaagcc attcgccagc gttttgtggg agatgttgct tatcctatga
    69061 tttccaatct tcccttctcg agcgaccgca agtggggggc tatggagtta gaaggcttgg
    69121 ggacagtttt cttaggggcg cctgagatgt tgctggattc tgaagtccca gaagccaggg
    69181 aggccttgga gagaggatca cgtgtcttgg tcttagctct cagtcaggag aaattagacc
    69241 atcacaaacc acagaaacca tctgatattc aggctctagc cttgctggaa atcttggact
    69301 ccattcgaga gggagcagca gagacgctgg actatctccg ttctcaggag gtaggactca
    69361 agattatctc tggtgacaat ccagttacgg tgtccagcat tgcccagaag gctggttttg
    69421 cggactatca caactatgta gattgctcaa aaatcaccga tgaggaattg atggccatgg
    69481 cggaggagac agctattttc ggacgtgttt cccctcatca aaagaaactc atcatccaaa
    69541 cgttgaaaaa agcgggtcat acaacggcta tgacagggga cggggttaat gatatcttgg
    69601 cccttcgtga ggcggattgt tctatcgtga tggcggaggg ggatccagca acccgtcaga
    69661 ttgccaatct ggttctcttg aactcagact ttaatgatgt tcctgagatt ctcttcgagg
    69721 gtcgtcgcgt ggtcaataac attgcccaca tcgccccgat tttcttgata aagaccatct
    69781 attccttcct gttagcagtc atctgtattg ccagtgcttt actaggtcgg tcagagtgga
    69841 ttttgatttt ccccttcatt ccgatccaga ttaccatgat tgaccagttt gtggaaggtt
    69901 tcccaccatt cgttctgact tttgagcgaa atatcaaacc tgttgagcag aatttcctca
    69961 gaaaatccat gcttcgtgcc ctaccaagcg ctctcatggt cgtctttagc gtcctgtttg
    70021 tgaaaatatt tgggagtagc caaggttggt ctgagttaga aatctcaact ctcctctatt
    70081 atctcttggg gtcaattggt ttcttatccg tatttagagc ctgcatgcca tttaccctat
    70141 ggcgtgtcct cttgattgtt tggtcagtag gaggtttcct agccacagct ctcttcccaa
    70201 gaatccaaaa actgcttgaa atttcaacct taacaggaca aacattacct gtttatggtg
    70261 tcatgatgtt ggtctttacc gtgattttca tcctgaccag tcgctatcaa gccagaaaat
    70321 aaagaaaggc tgcaatctgt ggattgcagt ttttttaggt acaagattgc cagtcgaaat
    70381 gtggtataat aagaggtaat agagttttgg aaagtgagag aagatgattt caaagagatt
    70441 agaattggta gcttcctttg tgtcacaggg ggctatttta ctagatgtgg gaagtgacca
    70501 tgcttatctg cctatcgagt tggttgagag aggccaaatc aaaagcgcta ttgcaggtga
    70561 ggtggtggaa ggtccctatc agtctgcggt taaaaatgtt gaggctcacg gcctaaagga
    70621 gaaaatccaa gtccgtttag ccaatggctt ggcagctttt gaagagactg accaagtgtc
    70681 tgtcattacc attgctggca tgggtggtcg tttgattgct aggattttag aagaaggttt
    70741 ggggaagtta gctaatgtag agcgtttgat cctccagccc aataatcgtg aagacgactt
    70801 gcgtatctgg ctacaggatc atggattcca gattgtagca gaaagcattt tagaagaagc
    70861 tggaaagttt tatgagattt tggtggtgga agcaggacaa atgaagctat cagccagtga
    70921 tgttcgcttt ggtcccttct tgtccaaaga agtcagtcca gtatttgtcc aaaaatggca
    70981 aaaagaagct gagaagctag agttcgccct cggacaaatc ccagaaaaaa atctggaaga
    71041 acgtcaagtt ctagtagata agattcaagc tatcaaggag gtgctccatg ttagcaagtg
    71101 aagtgatcca agcttatgaa gccttttgcc ctcaggaatt ttctatggag ggagacagcc
    71161 gtggtctgca aattggcact ttagacaagg gtatccaaag ggtcatggtt gccctcgata
    71221 ttcgtgaaga gacggtggca gaggctattg aaaagggtgt ggacttgatt atcgtcaagc
    71281 acgcgcctat ctttcgtcct atcaaggact tgcttgccag ccgtccgcaa aatcagattt
    71341 acatcgacct gattaagcat gatatcgcag tttatgtcag ccataccaat attgatatcg
    71401 ttgaaaatgg gctcaatgac tggttttgtc agatgctagg aatcgaggag acgacttatc
    71461 tgcaggaaac aggtccagaa cgtggaattg gacgtattgg gaatattcag cctcagacat
    71521 tttgggaatt ggcccaacag gtcaagcaag tctttgacct agatagcctt cgaatggtgc
    71581 attatcaaga gaatgatttg cagaagccta tttcaagagt agcaatttgt ggtggaagcg
    71641 ggcagtcttt ctataaggat gctttggcaa agggggcaga tgtctatatc actggtgata
    71701 tctactacca tactgctcag gatatgctgt ctgatggctt gttagcattg gatccaggtc
    71761 actatatcga agtgcttttt gtggaaaaaa tcgcagcact ccttactcaa tggaaagaga
    71821 aaaaaggctg ggaactagag attttaccta gtcaagcatc gaccaatcct ttccgtcata
    71881 tctagttaga aagtgaagac aatgaaaaaa gttgccatta ttggagcagg aattgtggga
    71941 gcaacagctg cctactacct ctcgagagaa agtgacctag aggtgaccgt ttttgaccat
    72001 gagcaaggtc aagccaccaa ggccgcagca ggaattatca gtccttggtt ttccaaacgc
    72061 cgtaataaag cctggtacaa gatggcgcgc ttgggggctg atttttatgt ggatttatta
    72121 gctgatttag agaaatcagg acaagaaatc gacttttacc agcgttcggg agtctttctc
    72181 ttgaaaaagg atgaatccaa gttggaagaa ctctatcaac tggctctcca gcgcagagaa
    72241 gaatctcccc tgataggcca gttagccatt ctggatcaag catcagctaa tgaattattc
    72301 cctggcttgc agggattcga caacttgctc tatgcttctg gtggagcgag agtagatggc
    72361 caactcttgg taactcgtct gctagaagcc agtcatgtca agttggtcaa agaaaaagtg
    72421 actctgacac tcttatcatc aggctaccag attggcgaag aggtgtttga tcaggttatt
    72481 ttggcaacgg gagcttggtt gggggacatg ttagaacctt taggttatga agtggatgtc
    72541 cgtcctcaaa aaggacaact acgagattat cagcttgccc aagacatgga agattaccct
    72601 gttgtcatgc cagaagggga gtgggatttg attccctttg caggtgggaa attatccttg
    72661 ggcgctaccc atgaaaatga catgggattt gatttgacgg tagatgaacc cttgctccaa
    72721 caaatggagg aggcggcctt gcctcattac ccagttttag ctagagtgac ttcaagggct
    72781 gagcgtgtgg gaatccgtgc ctataccagt gatttctctc ccttctttgg gcaggtgcca
    72841 gaattggcag gtgtctatgc agccagtgga ctaggttcat caggcctcac aactggtcct
    72901 atcattggtt accatctagc ccaactgatc caagacaagg agttgacctt ggaccctcta
    72961 aattacccaa ttgaaaacta tgtcaaacga gtaaaaagcg aataatactc aatgaaaatc
    73021 aaagaacaaa ctaggtaact agccgcgggt tactcaaagc actgctttga ggttgtggat
    73081 agaactgacg aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc
    73141 agtaaccata cccatggcaa ggcgacgttg acgtggtttg aattggattt tcgaagagta
    73201 taagcatttt actgaaattt tatcaggtag tttaggatgg caaatgacat tccctgtcaa
    73261 aaatggtaaa ataagaaaaa ataatctgag aatcgaggaa aaaagatgca agaaaagatt
    73321 ttggtaactg gtggtgccgg ttttattgga acccacactg ttattgaatt gatccaagca
    73381 ggccatcaag ttgttgtggt ggataatctt gtcaatagca atcgtaagag tttagaagtt
    73441 gttgaaagaa tcacaggagt tgagattcct ttctatgagg cagatattcg tgacacagct
    73501 accctcagag atattttcaa gcaggaagaa ccgactggtg tcattcactt tgctggtttg
    73561 aaggctgttg gcgaatcaac acgtatccct cttgcctact atgacaacaa tatcgctgga
    73621 actgtcagcc ttttgaaagc catggaagaa aacaactgta aaaacatcat cttcagttct
    73681 tctgcgacag tttacgggga tccgcacaca gtgcccatct tggaagattt cccactttca
    73741 gtgaccaacc catacggtcg tactaagctc atgctagagg aaattttgac tgatatttac
    73801 aaagcagact cagaatggaa tgttgtcttg cttcgttact ttaacccaat cggagcccat
    73861 gagagtggtg atttgggaga aaatccaaac ggtattccaa acaatctctt gccatatgtg
    73921 actcaagtag ccgttggaaa attagagcaa gtgcaagtgt ttggagacga ttacgatacg
    73981 gaagatggaa caggtgttcg tgactatatc cacgttgtcg atttggctaa gggtcacgtt
    74041 gcagctttga aaaaaatcca aaaaggttca ggactaaacg tttataacct tggaactggt
    74101 aaaggttact cagttcttga aattatccaa aacatggaaa aagcggtggg ccgtcctatt
    74161 ccttaccgca tcgtagaacg tcgcccaggt gatatcgctg cctgctactc agacccggca
    74221 aaagctaaag cagaactcgg ttgggaagca gaactcgaca tcacccaaat gtgtgaagac
    74281 gcatggcgtt ggcaaagcaa gcatccaaat ggatttgaag actaagatga tgatttcaat
    74341 catcgtccct tgtttaaacg aagaggaagt acttcctctt ttttatcagg ctctggaagc
    74401 tttacttcca gatttggaaa cagaaatcga gtatgtcttt gtcgatgatg gatcaagtga
    74461 tgggaccttg gaactcttaa aggcctatcg ggagcaaaat ccggcagtcc attatatttc
    74521 tttctctcga aattttggca aagaagcagc cctttatgca ggcttgcaat atgcgacagg
    74581 agatttggtg gtggtgatgg atgcagacct ccaagatcct cctagtatgt tgtttgagat
    74641 gaaaaatgta ctagacaaaa atgtagactt ggactgcgtt gggacacgga gaactagtcg
    74701 ggagggagaa cccttctttc gcagtttctg tgctgttctc ttttatcgcc tcatgcaaaa
    74761 aatcagccca gtagctctgc cgtcgggtgt ccgtgatttt cgtatgatga gaaggtctgt
    74821 agtcgatgcc attttaagct tgaccgagtc caatcgtttt tcaaaggggc tcttcgcctg
    74881 ggtgggtttc aaaacctact atttggacta tccaaatgtc gaaaggcagg ctggcaagac
    74941 cagttggagt tttaggcaac tcttttttta ctccattgaa gggattgtta atttttcaga
    75001 tttccctttg actatagcct ttgtagctgg tctcctatct tgttttcttt ctctgctgat
    75061 gacctttttt gttgtggttc ggaccctcat tttgggcaat ccgacatctg gttggacctc
    75121 tctgatggct gttattctct ttcttggagg cattcaactc ttgaccattg ggattctcgg
    75181 taagtatatc agtaagattt atttagagac taaaaaaaga ccactttatc ttatcaaaga
    75241 aaaaagtgac cttcctgatt ttacagaaaa aataaagtga aaagactata attttacatg
    75301 gaaatgtgct aaactagagg gagtggatta tcgccattga tttaggcttc ctggtttcca
    75361 atcagggcgc gagttgggca atttttaaca gcctctaaga catcctgact aggagaaatt
    75421 tctttttcca gttggtcagg gtcatcgtaa aaacgcacga ttccattatc gtggtaatca
    75481 aataaatcag aataagtttg gcaaagtcca caggcgatac atcgttcagg tataagtgtg
    75541 attttcatgt tcatatttat attgtaataa gaaaagtaaa aaaaaacaag gagtaaggta
    75601 tggcaaaaga accgtggcaa gaagatattt atgatcaaga agaatcaaga gcagggcgtc
    75661 ggcatcgaaa ccacggaggg gctgatagga tggctaatcg tattttgacg atcctagcta
    75721 gtattttctt tgtaattgtg gtggtgatgg tcatcgttct catctatcta tcatcggggg
    75781 ggagtaatcg cacagcagcc ttaaaagact ttcatgattc tgatgcaagt gtagtacaaa
    75841 tctcatcttc aagcagttct cagcctgagc agagttcaga gccagaatct acttctagta
    75901 gttcagaaga agctgctaat cctgaaggaa cgattaaagt tctcgcagga gaaggggaag
    75961 cagctattgc cgctcgtgca ggaatctcca ttgctcagtt agaggccttg aatcctgggc
    76021 acatggctac aggatcttgg tttgctaatc caggtgatgt tataaaaata aaataggagc
    76081 cgatatgaaa acaattcaaa ttgctattga tggtcctgct tccagcggta agagtacggt
    76141 cgcaaagatt attgctaagg attttgggtt cacctacctt gatacaggag ctatgtatcg
    76201 tgcagcgacc tatatggctc ttaagaacca attaggagtt gaagaagtcg aagaccttct
    76261 agccttgttg gaccagcatc caatcagctt tggacgttca gaaactggag accagcttgt
    76321 ttttgtagga gatgtggata ttacccatcc tatccgtgaa aatgaagtga ccaatcatgt
    76381 ttctgctatt gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc aacaagaaat
    76441 tgcccagcaa ggcgggattg tcatggatgg tcgcgatatt ggaactgttg tattgccaca
    76501 agcagaattg aaaattttcc tagtagcttc tgttgatgag agagcagagc gtcgttacaa
    76561 ggaaaatatt gccaagggaa ttgaaacaga ccttgaaacc ctaaaaaagg aaattgctgc
    76621 gcgtgactac aaggatagtc atcgtgagac ttctcctctc aaacaagcag aggatgctgt
    76681 ctaccttgat acaactggtt tgaacattca agaagtagtt gaaaaaatca aagcagaagc
    76741 tgaaaaaaga atgtaaatgt agaaaagccg atagattgat atcggttttt ttctaaattt
    76801 gtcaaaatgc tgattttagg gtataatagt tgctgttcga gaaattttga ttttcaaaga
    76861 atagtgaatg atgataagga gaaaccatga acaatttacc aaattgtcca aaatgtaact
    76921 cagagtatgt ctacgaagac ggtgccctac tggtttgccc agagtgtgct catgagtgga
    76981 atcctgctga agttgcagaa gtagaagaag gtcttgtcgc tatcgatgcc aacggaaata
    77041 aattgactga tggtgataca gtaactctca tcaaggactt gaaagtaaaa ggtgcgccaa
    77101 aagatttgaa acaagggacg cgcgtgaaaa atatccgcat cgtagaaggc gatcacaata
    77161 tcgactgtaa gattgatggt tttggtgcca tgaaacttaa atcagagttt gtgaggaaga
    77221 tttaagcatg tcaaaacact ataaacttgt attttatagc cgtatcttct tgtttctagc
    77281 ggcttttacg ggagtttatc ttgaaattac taggcatggt ggttttggga tgcttctcta
    77341 ttacacggtt ctgtccaacc tcttggtaac tatttttacc ctttatcttc taaaggttat
    77401 gagccgtgta ggtgaaaatt ggcaaaggcc aagtctcttg cgcttaaaag gtggggtcac
    77461 catgagtatc atgattacct gtgtgattta ccatttcctc ttggcgccca ttgcgactaa
    77521 tttctatacc ctagaaaatt tcctttgcca ctatatcgtt cccatctggt ttttagcgga
    77581 taccctcttt tttgacaaac agggtcaata caagatttgg gatccagcag tgtggaccat
    77641 tttacccttt ctgtatatga tgtttgctct ttttaatggc ttggttctaa aactcaatat
    77701 tccaaatgcc aaggataatc ctttccctta cttctttttg aatgtgaaca agggttggaa
    77761 tgttgtgttt aagtggtgtc tgattatctt tgttgcctat atggtagcag gatttatttt
    77821 ctactttatc aagcaaatca agagaaagtc atcctaatac tcttcgaaaa tctcttcaaa
    77881 ccacgttagt ttccatctgc aacctcaaaa cactgttttg aacaacctgc ggctagcttt
    77941 ctagtttgct ctttgatttt cattgagtat gagatacaag ggattcattt tcgaatttaa
    78001 gaaggagtct acaaaccaat gaggcttctt cttttcttgc ttatttgata actatcaaaa
    78061 aagtagaaga ttaagagaaa tatagaaaga gatttcatga aacaattttt agaacgggcc
    78121 agcattttgg ctctctccct cgttttgatt acctcctttt ccatttcgag tgccctacca
    78181 gccatgtttg actattatca gggttattct aaggaacaaa ttgagctctt ggtgagcttg
    78241 ccttcctttg gaatcatgat gatgttactg ctaaatggtt tcttagaaaa aatatttcct
    78301 gagcgcttac agattagttt gggcttgctg attttatcat tgagcggtac agctcccttc
    78361 tggtaccaag cctatccctt tgtctttgga acacggcttc tctttggttt gggtcttggg
    78421 atgatcaatg ccaaggccat ttctattatc agtgaacgct accaaggaaa aaggcgaatt
    78481 cagatgttag ggctacgcgc ttctgcagag gtcgttggag cttctctcat taccttggcc
    78541 gtcggtcagt tgttggcctt tggttggaca gctatctttc tagcctatag tgctggattt
    78601 ttggtgatgc ccctttatct gctctttgtc ccttatggaa aatcaaagaa agaagtcaag
    78661 aaaagagcga aggaagcaag tcgtttaact cgagaaatga aaggcttgat ttttacctta
    78721 gctatcgaag cggcagttgt agtttgtacc aatacagcta ttaccatccg tattccaagt
    78781 ttgatggtgg aaagaggatt gggggatgcc cagttatcta gttttgttct tagtatcatg
    78841 cagttgatcg ggattgtggc tggggtgagt ttttctttct tgatttctat ctttaaagag
    78901 aaactgctcc tctggtctgg tattaccttt ggcttggggc aaatcgtgat tgccttgtct
    78961 tcatccttgt gggtggtagt agcaggaagt gttctggctg gatttgccta tagtgtagtc
    79021 ttgacgacgg tctttcaact tgtctctgaa cgaattccag ctaaactcct caatcaagca
    79081 acttcatttg ctgtattagg ctgtagtttc ggagccttta cgaccccatt tgttctaggt
    79141 gcaattggct tactaactca caatgggatg ttggtcttta gtatcttagg aggttggttg
    79201 attgtaatct ctatctttgt catgtaccta cttcagaaga gagcttagga ttgattccta
    79261 agtttttctt ttgatactca atgaaaatca aagagcatag ttgattgagt ttggaatagt
    79321 acgctgtagc tgctaaaata tttctagaaa ttaatttgac ttttctaata gagttattca
    79381 tatcttattt caatctacta tagaaagcta gccgcaggtt gctcaaaata gtgttttgag
    79441 gttgcagatg gaaactgacg tggtttgaag agattttcga agagtatgag atttttatac
    79501 ccatacaata tttgttttag gtccttgttt acagactaat ttcttgataa aatagaggtt
    79561 atgacgagat ataaagcaac tatttcctat gatggttatg cctttgctgg ctttcagcgc
    79621 cagtctcatg cgcgtagcgt tcaggaagaa attgaaaaaa ccttgaccag attaaataaa
    79681 gggcaaacca ttactgttca cggtgctggt aggacagata gtggggttca tgccctggga
    79741 caggtcattc attttgacct gccttatcag atggatgagg agaaactccg ttttgccttg
    79801 gatacccagt ctcctgaaga tattgatgtg atttcgattg agcttgtggc agatgatttt
    79861 cattgccgtt atgccaaaca tagcaagacc tatgagttta ttgtggatag aggacgtccc
    79921 aaaaatccta tgcgccgtca ctatgccacc cactttccct acccactcga tgtggaacga
    79981 atgcagattg caatcaaaaa gctagaggga acccatgatt ttaccggttt tacagcctct
    80041 gggactagtg tagaggataa ggttcgcacc atcacagaag ctagtttaat agtcgatgag
    80101 acaggacaat ttttgacctt taccttttca ggaaatggtt tcttgtataa acagattcgc
    80161 aatatggtgg ggactcttct caaaatcggt aacaaccgca tgccagtaga gcagattgat
    80221 ctcatcttgg agaagaagga caggcaactt gcaggtccca ctgcagcacc aaatggtttg
    80281 tatttaaagg agattcgtta tgaagaataa tcgtatttta gcactttctg gaaatgatat
    80341 ttttagtggt ggtggactgt cagctgattt ggctacctat accttgaacg gcttgcatgg
    80401 gtttgtagca gtgacttgtt tgacagcctt gacagaaaaa ggatttgaag tctttccaac
    80461 tgatgatacc atttttcaac atgaattaga tagcttacgt gatgtggaat ttgggggaat
    80521 taagattggt cttctcccta ctgtcagtgt ggctgagaag gccttggact ttatcaaaca
    80581 acgcccagga gtacctgtgg tgttggatcc tgtcttggtc tgcaaggaaa cgcatgatgt
    80641 agctgtcagt gagctctgcc aagagttgat tcgctttttc ccttatgtca gtgtgattac
    80701 gcctaatctc ccagaagcag aattattatc cggtcaggaa attaaaacct tggaagacat
    80761 gaaaactgca gcgcagaaat tgcatgattt aggagcgcca gcagtcatta tcaagggagg
    80821 caatcgtctt agtcaggaca aggctgtgga tgtcttttat gatggacaga cctttactat
    80881 cctagaaaat ccagttatcc aaggccaaaa tgctggtgca ggttgtacct ttgcctctag
    80941 cattgccagt cacttgatta aaggtgataa acttttgcca gcagtagaaa gctctaaggc
    81001 tttcgtttat cgtgctattg cacaagcaga tcagtatgga gtaagacaat atgaagcaaa
    81061 caaaaacaac taaaatcgcc cttgtatccc tattaaccgc cctttctgtg gttctaggtt
    81121 atttcttaaa aatcccaaca cctacaggaa ttctaactct tttagatgct ggtgtcttct
    81181 ttgcggcctt ttactttggt agtcgtgaag gagcggtagt cggaggacta gcaagtttct
    81241 tgattgacct cttatcaggc taccctcagt ggatgttctt tagcttggtc aaccatggct
    81301 tgcagggatt tttcgcagga tttaaaggaa aaagtcagtg gttaggcctt attttagcaa
    81361 ctattgccat ggtaggaggc tacgccttgg gttctacttt gatgaatggc tgggcagcag
    81421 ccctcccaga aattctacca aatttcatgc aaaatatggt agggatgatt gtaggattta
    81481 ttcttagtca aagtatcaag aagattaagt aatactcttc gaaaatctct tcaaaccacg
    81541 tcagcttcca tctgcaacct caaaacactg ttttgagcag cctgaggcta gctttctagt
    81601 ttgcactttg attttcattg agtataaaga ggttgagtaa aaagtatttc aaataagaaa
    81661 aacgcatagt atcaggtgtt gaatgaactg ccccccaaaa gttagacaga aaaaatctaa
    81721 cttttggggt gttttattat gaaactaact tatgatgata aagttcagat ctatgaactt
    81781 agaaaacaag gatatagctt agagaagctt tcaaataaat ttgggataaa caattctaat
    81841 cttaggtaca tgattaaatt gattgatcgt tacggaatag agttcgtcaa aaaagggaaa
    81901 aatcgttact attctcctta tttaaaacaa gaaatgatt
//
LOCUS       DBERYS010000004        76059 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.004, whole genome shotgun sequence.
ACCESSION   DBERYS010000004 DBERYS010000000
VERSION     DBERYS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 76059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76059
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.004"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            145..6846
                     /gene="bgaA"
                     /locus_tag="ACL8LA_000300"
     CDS             145..6846
                     /gene="bgaA"
                     /locus_tag="ACL8LA_000300"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HGS1812129.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7094..7540)
                     /locus_tag="ACL8LA_000301"
     CDS             complement(7094..7540)
                     /locus_tag="ACL8LA_000301"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1812130.1"
                     /translation="MFDKEQKRGYKQSAKNLANWHYNDKEDSYIHPDGWCYRFHHIKY
                     QKTQTDFQQEIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQDRQIFAQRKI
                     DVEPVFGQIKACLGYKRCNLRGKRQVRIDMGLVLMANNLLKHSEMK"
     gene            complement(7705..7950)
                     /locus_tag="ACL8LA_000302"
     CDS             complement(7705..7950)
                     /locus_tag="ACL8LA_000302"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1812131.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTLEERHFYTS
                     YHAFGRPSYHPKMLVSTLLFAYSQGIFSGRKIEKWKS"
     gene            complement(8161..8520)
                     /locus_tag="ACL8LA_000303"
     CDS             complement(8161..8520)
                     /locus_tag="ACL8LA_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HGS1812132.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            8612..9568
                     /locus_tag="ACL8LA_000304"
                     /pseudo
     CDS             8612..9568
                     /locus_tag="ACL8LA_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9552..10109
                     /locus_tag="ACL8LA_000305"
     CDS             9552..10109
                     /locus_tag="ACL8LA_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1812133.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10102..10356
                     /locus_tag="ACL8LA_000306"
     CDS             10102..10356
                     /locus_tag="ACL8LA_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812134.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10368..12422
                     /locus_tag="ACL8LA_000307"
     CDS             10368..12422
                     /locus_tag="ACL8LA_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGS1812135.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12601..13461
                     /locus_tag="ACL8LA_000308"
     CDS             12601..13461
                     /locus_tag="ACL8LA_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGS1812136.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            13672..14382
                     /gene="ccdA2"
                     /locus_tag="ACL8LA_000309"
     CDS             13672..14382
                     /gene="ccdA2"
                     /locus_tag="ACL8LA_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS1812137.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14402..14968
                     /locus_tag="ACL8LA_000310"
     CDS             14402..14968
                     /locus_tag="ACL8LA_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HGS1812138.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14979..16091
                     /gene="msrB"
                     /locus_tag="ACL8LA_000311"
     CDS             14979..16091
                     /gene="msrB"
                     /locus_tag="ACL8LA_000311"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS1812139.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16359..17096
                     /locus_tag="ACL8LA_000312"
     CDS             16359..17096
                     /locus_tag="ACL8LA_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1812140.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17093..18784
                     /locus_tag="ACL8LA_000313"
     CDS             17093..18784
                     /locus_tag="ACL8LA_000313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1812141.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            18900..19463
                     /locus_tag="ACL8LA_000314"
     CDS             18900..19463
                     /locus_tag="ACL8LA_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HGS1812142.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            19485..25154
                     /locus_tag="ACL8LA_000315"
     CDS             19485..25154
                     /locus_tag="ACL8LA_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1812143.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEALKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            25215..26936
                     /gene="pabB"
                     /locus_tag="ACL8LA_000316"
     CDS             25215..26936
                     /gene="pabB"
                     /locus_tag="ACL8LA_000316"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGS1812144.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            27467..28582
                     /locus_tag="ACL8LA_000317"
     CDS             27467..28582
                     /locus_tag="ACL8LA_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS1812145.1"
                     /translation="MSKGDFIDPKTQFKHQYRGESPVFRIRNRKYVELGTTDKKLDER
                     RDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSVNSNLLNSFGEKAKKVLIRSKL
                     INVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDLTDGEYKYVAYESQQYTDPQTT
                     LTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPKPDEKPKPSAPQQEKTKPTVQS
                     GWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYVQNAWVGNYYLKSGGYMAKSEW
                     IYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAKSEWVYDSNYKSYYYLTSEGSY
                     ARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK"
     gene            28669..29588
                     /locus_tag="ACL8LA_000318"
                     /pseudo
     CDS             28669..29588
                     /locus_tag="ACL8LA_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
     gene            29723..30682
                     /locus_tag="ACL8LA_000319"
     CDS             29723..30682
                     /locus_tag="ACL8LA_000319"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGS1812146.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            30779..31618
                     /locus_tag="ACL8LA_000320"
     CDS             30779..31618
                     /locus_tag="ACL8LA_000320"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGS1812147.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(31666..31836)
                     /locus_tag="ACL8LA_000321"
     CDS             complement(31666..31836)
                     /locus_tag="ACL8LA_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGS1812148.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            31957..32841
                     /gene="miaA"
                     /locus_tag="ACL8LA_000322"
     CDS             31957..32841
                     /gene="miaA"
                     /locus_tag="ACL8LA_000322"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGS1812149.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            32834..34072
                     /gene="hflX"
                     /locus_tag="ACL8LA_000323"
     CDS             32834..34072
                     /gene="hflX"
                     /locus_tag="ACL8LA_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGS1812150.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            34065..34688
                     /locus_tag="ACL8LA_000324"
     CDS             34065..34688
                     /locus_tag="ACL8LA_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGS1812151.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            34703..35632
                     /gene="rnz"
                     /locus_tag="ACL8LA_000325"
     CDS             34703..35632
                     /gene="rnz"
                     /locus_tag="ACL8LA_000325"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGS1812152.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            35653..36408
                     /locus_tag="ACL8LA_000326"
     CDS             35653..36408
                     /locus_tag="ACL8LA_000326"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1812153.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(36462..37370)
                     /locus_tag="ACL8LA_000327"
     CDS             complement(36462..37370)
                     /locus_tag="ACL8LA_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS1812154.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            37434..37703
                     /locus_tag="ACL8LA_000328"
     CDS             37434..37703
                     /locus_tag="ACL8LA_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGS1812155.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            37703..38083
                     /locus_tag="ACL8LA_000329"
     CDS             37703..38083
                     /locus_tag="ACL8LA_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS1812156.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            38172..38399
                     /locus_tag="ACL8LA_000330"
                     /pseudo
     CDS             38172..38399
                     /locus_tag="ACL8LA_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38439..39164)
                     /locus_tag="ACL8LA_000331"
     CDS             complement(38439..39164)
                     /locus_tag="ACL8LA_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HGS1812157.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            39326..41167
                     /gene="typA"
                     /locus_tag="ACL8LA_000332"
     CDS             39326..41167
                     /gene="typA"
                     /locus_tag="ACL8LA_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGS1812158.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            41187..41441
                     /locus_tag="ACL8LA_000333"
     CDS             41187..41441
                     /locus_tag="ACL8LA_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGS1812159.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
     gene            41806..42105
                     /locus_tag="ACL8LA_000334"
                     /pseudo
     CDS             41806..42105
                     /locus_tag="ACL8LA_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            42188..44209
                     /locus_tag="ACL8LA_000335"
                     /pseudo
     CDS             42188..44209
                     /locus_tag="ACL8LA_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            44211..44852
                     /locus_tag="ACL8LA_000336"
     CDS             44211..44852
                     /locus_tag="ACL8LA_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812160.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            44956..46308
                     /gene="murD"
                     /locus_tag="ACL8LA_000337"
     CDS             44956..46308
                     /gene="murD"
                     /locus_tag="ACL8LA_000337"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGS1812161.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            46312..47370
                     /locus_tag="ACL8LA_000338"
     CDS             46312..47370
                     /locus_tag="ACL8LA_000338"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGS1812162.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            47380..48579
                     /gene="divIB"
                     /locus_tag="ACL8LA_000339"
     CDS             47380..48579
                     /gene="divIB"
                     /locus_tag="ACL8LA_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGS1812163.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            48812..49513
                     /gene="pyrF"
                     /locus_tag="ACL8LA_000340"
     CDS             48812..49513
                     /gene="pyrF"
                     /locus_tag="ACL8LA_000340"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGS1812164.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            49547..50179
                     /gene="pyrE"
                     /locus_tag="ACL8LA_000341"
     CDS             49547..50179
                     /gene="pyrE"
                     /locus_tag="ACL8LA_000341"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGS1812165.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            50412..50762
                     /locus_tag="ACL8LA_000342"
     CDS             50412..50762
                     /locus_tag="ACL8LA_000342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812166.1"
                     /translation="MFLLTVLARQIGDYDLTLPRWGSDTTSELEKENASAGINNNDST
                     GGGKRLNTSIRSAYSGSDITPVYSLGSGSRIVMYYNGGGDNYIGSGTRLAMAPQFGNH
                     VRIHTSGSWNPDSY"
     gene            50858..51622
                     /locus_tag="ACL8LA_000343"
     CDS             50858..51622
                     /locus_tag="ACL8LA_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812167.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            51622..52374
                     /locus_tag="ACL8LA_000344"
     CDS             51622..52374
                     /locus_tag="ACL8LA_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812168.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            52384..53082
                     /locus_tag="ACL8LA_000345"
     CDS             52384..53082
                     /locus_tag="ACL8LA_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812169.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            53079..53726
                     /locus_tag="ACL8LA_000346"
     CDS             53079..53726
                     /locus_tag="ACL8LA_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812170.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(53806..54667)
                     /locus_tag="ACL8LA_000347"
                     /pseudo
     CDS             complement(53806..54667)
                     /locus_tag="ACL8LA_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(54679..55437)
                     /locus_tag="ACL8LA_000348"
     CDS             complement(54679..55437)
                     /locus_tag="ACL8LA_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1812171.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(55438..56115)
                     /locus_tag="ACL8LA_000349"
     CDS             complement(55438..56115)
                     /locus_tag="ACL8LA_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1812172.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(56075..56743)
                     /locus_tag="ACL8LA_000350"
     CDS             complement(56075..56743)
                     /locus_tag="ACL8LA_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1812173.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            56969..57103
                     /locus_tag="ACL8LA_000351"
                     /pseudo
     CDS             56969..57103
                     /locus_tag="ACL8LA_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            57243..58733
                     /gene="lysS"
                     /locus_tag="ACL8LA_000352"
     CDS             57243..58733
                     /gene="lysS"
                     /locus_tag="ACL8LA_000352"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGS1812174.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            59358..60494
                     /gene="lctO"
                     /locus_tag="ACL8LA_000353"
     CDS             59358..60494
                     /gene="lctO"
                     /locus_tag="ACL8LA_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGS1812175.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLRLY"
     regulatory      60748..60836
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            61051..61719
                     /locus_tag="ACL8LA_000354"
     CDS             61051..61719
                     /locus_tag="ACL8LA_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGS1812176.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            61956..62738
                     /gene="thiM"
                     /locus_tag="ACL8LA_000355"
     CDS             61956..62738
                     /gene="thiM"
                     /locus_tag="ACL8LA_000355"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS1812177.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPE
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            62740..63369
                     /gene="thiE"
                     /locus_tag="ACL8LA_000356"
     CDS             62740..63369
                     /gene="thiE"
                     /locus_tag="ACL8LA_000356"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS1812178.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      63578..63666
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            63928..64488
                     /locus_tag="ACL8LA_000357"
     CDS             63928..64488
                     /locus_tag="ACL8LA_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1812179.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            64489..65874
                     /locus_tag="ACL8LA_000358"
     CDS             64489..65874
                     /locus_tag="ACL8LA_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1812180.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            65876..66526
                     /locus_tag="ACL8LA_000359"
     CDS             65876..66526
                     /locus_tag="ACL8LA_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGS1812181.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            66537..67229
                     /gene="tenA"
                     /locus_tag="ACL8LA_000360"
     CDS             66537..67229
                     /gene="tenA"
                     /locus_tag="ACL8LA_000360"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGS1812182.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            67234..67758
                     /gene="thiW"
                     /locus_tag="ACL8LA_000361"
     CDS             67234..67758
                     /gene="thiW"
                     /locus_tag="ACL8LA_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGS1812183.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            67758..68561
                     /locus_tag="ACL8LA_000362"
     CDS             67758..68561
                     /locus_tag="ACL8LA_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS1812184.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            68554..69186
                     /gene="thiE"
                     /locus_tag="ACL8LA_000363"
     CDS             68554..69186
                     /gene="thiE"
                     /locus_tag="ACL8LA_000363"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS1812185.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(69396..70187)
                     /gene="thiD"
                     /locus_tag="ACL8LA_000364"
     CDS             complement(69396..70187)
                     /gene="thiD"
                     /locus_tag="ACL8LA_000364"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS1812186.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(70271..70369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            70553..70948
                     /locus_tag="ACL8LA_000365"
     CDS             70553..70948
                     /locus_tag="ACL8LA_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGS1812187.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            70959..71330
                     /locus_tag="ACL8LA_000366"
     CDS             70959..71330
                     /locus_tag="ACL8LA_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGS1812188.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            71340..73583
                     /locus_tag="ACL8LA_000367"
     CDS             71340..73583
                     /locus_tag="ACL8LA_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS1812189.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            73790..75565
                     /gene="spxB"
                     /locus_tag="ACL8LA_000368"
     CDS             73790..75565
                     /gene="spxB"
                     /locus_tag="ACL8LA_000368"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGS1812190.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            75676..76023
                     /locus_tag="ACL8LA_000369"
     CDS             75676..76023
                     /locus_tag="ACL8LA_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1812191.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT        23186 a        13750 c        16744 g        22379 t
ORIGIN      
        1 catatctacg gcaaggcgac gctgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa atgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggca ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 aatttcgatc atgaatctcc tgcacaaaat gaaggtggac aactcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc tatccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac gtggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggcaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcatcagc aacccgtaca
     2101 cgaggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccaggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc acccttactg ctcactctga tctcttgaaa
     3061 tctagtcaag tcactgtctt tactggtaag aaagaaggac aagaaaagac tgttttgggg
     3121 acagaggtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
     3181 ccgtttctat acagtgatgg tagtcgtgca gaacgtcctg taacctggtc ttcagtagat
     3241 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
     3301 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaagca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ttgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcaag catatttgtt cagcctaaag atggtggccc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagctt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagtgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag actcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgtcc tattcggaga ttcaggtatt
     4741 ttgagcaaac gttcagttga taacttaagc gttggcttcc atgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtga gagcagacaa caaactagga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa gcaaggacaa
     5101 acaagaatcc aagttgacgg taaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttacg gcagtcacag caagtgttag caacaatggc
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccgg ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaaaac
     5461 ctgacagttg aatgggaaga agttccagcg gaaaatctga taaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgct
     5821 caagccaaac ttcacttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac caagtgaatt gcctcaagaa agcacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgaga agacagttgc tgctgtacaa
     6421 gaagatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
     6481 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa aactggtaaa
     6541 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     6601 gtagaagctc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     6661 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     6721 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
     6781 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     6841 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
     6901 tagaagataa agagtctgga acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     6961 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt agattgaaac
     7021 tagagtagta cacttctact tctaaaacat tgttagaaat cgatttgact gtccttatcg
     7081 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
     7141 atcccatgtc aattctcact tgacgcttgc ctctcagatt acatctcttg taacccaaac
     7201 aagcctttat ctgcccaaag acaggttcta catcaatctt gcgttgagcg aaaatctgtc
     7261 tatcttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
     7321 tcatatacaa tcccttttga ggggctgatt caggttcatc agcgtagtaa accttgattt
     7381 cctgttgaaa gtctgtctgt gttttctggt acttgatatg gtgaaaacga tagcaccagc
     7441 catcaggatg tatgtagcta tcctccttgt cattatagtg ccaattcgct aagtttttag
     7501 ctgactgttt ataccctctc ttttgttcct tatcaaacat ggcatattta atcagaaggg
     7561 gagtgatttc ttcttgaaaa tagacttgta tctgttcttg aagtttggcg gagaatttct
     7621 ctgtcgtttt cttccacaca aaactatact tgttggcatt ggattcaatc ttagttccgt
     7681 caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc gaccagagaa
     7741 aatcccttgt gaataggcaa atagaagagt agatacaagc attttagggt gataagacgg
     7801 gcgaccaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat tcaccacttt
     7861 ttcaatagta aagacgagat ggtcttgtgg taagaaggaa ctgatttcta gtggtaaagt
     7921 tgtttgattt gtgttatagt gaatatgcat gggatagcct ttctaaatgg tttattaagc
     7981 aattctattt taccaagact atcgtaacca tgcaaaaaag caacggcaaa tccgttgcct
     8041 tttttggata taagagacta ttgtcccaga ctcttttctt tgtataaaat tgtatagtgt
     8101 tattgtctat actattttgt agagaaagca aaaatcccta gaaaatgctt agggattcga
     8161 ctatttcatt aagaacaatt caacaatcat ctctatatac atgataaggg ttaataaaaa
     8221 tcctgtacgc cagaagaatt tgatgaattt aggatagtaa aagctgcgtt tttttaaaag
     8281 gaagaaaaag acgagaataa tggctaggaa cgagagggct agccccagtc tagggaggaa
     8341 attatgggta aaggttttag ctgtaatcaa gtaatactca aataccaaga ctggaaaagc
     8401 caaatccgca aagtttcgtc ctagtttttt taatctaaaa agtttggtta ggataatgca
     8461 gacgactaag gtcagtatca ataataaaat agatgctaat ttcattaaaa tcatacccat
     8521 attgtatcat aaaaaaaggc taatggaaac gagaaacaga ggaatttata ggaaagtcag
     8581 gcttttgaga ttgtgggtga tttttgttat aatgaaagtt ataaaatctt atgatacctt
     8641 gaatgattat tatcgaaaac tctttggaga aaagactttt aaagtcccta ttgatgcggg
     8701 atttgactgt cccaatcgtg atggaactgt ggctcatgga ggctgtactt tttgtacggt
     8761 ttccggttct ggagatgcca ttgtagcccc agatccgcct atccgtgagc aattttataa
     8821 ggaaattgac tttattcatc gtaagtggcc agatgttcag aagtatctgg tttattttca
     8881 aaattttacc aacacccatg aaaaggtgga agttattcga gagcgttatg aacaggctat
     8941 caatgagcca ggtgtggtag gaatcaatat tggaacgcgc ccagactgtt taccagacga
     9001 aaccatcgaa tatttggctg agttatcgga gtgcatgcat gtgacggttg aattgggctt
     9061 gcagactact tatgaagcaa cctctgacct gattaaccgt gcgcattctt atgaattgta
     9121 ggtggaaacg gtcaagcgtt tgagaaagta tccaaaaatt gagattgttt cccatttgat
     9181 caatggtttg cctggtgaaa cccatgagat gatggttgaa aatgtccgcc gttgtgtcac
     9241 ggataacgat attcaaggga ttaaactgca tttgctccat cttatgacaa atactcgtat
     9301 gcaacgagat taccatgagg gacgtttaca attgatgagt caggacgaat atgtcagggt
     9361 catctgtgac caactggaaa ttattcccaa acatatcgtc atccatcgaa ttacaggaga
     9421 tgcgcctagg gatatgctga ttggtcctat gtggagcctc aataaatggg aagtcctcaa
     9481 cagcattgag atggagatga gacgtcgtgg aagtgttcaa ggatgcaagg ctgtaaaaca
     9541 ggagtttgaa aatgaaaaga ccacttgaga tggcacatga ttttttggct gaggtcgtga
     9601 caaaagagga tgtcgtagtg gatgcgacta tgggaaatgg tcatgacacg ctttttttag
     9661 ccaagctagc caagcaagtc tatgcctttg atattcagaa gcaagccttg gaaaagaccc
     9721 aagagcgttt gcatcaggct ggcttgacaa atgcccagtt aatcttgcaa ggccatgaga
     9781 cactggacca gtttgtgaca aaagctaagg cagggatttt taatttgggc tatttgccgt
     9841 cagctgataa gtctgtcatc acccgaccgc agacaacgat tgaggcatta gaaaagctat
     9901 gtggcttact tgtcaaaggt ggacgaattg ctattatgat ttactatggt catgaaggag
     9961 gcgacctcga gagagatgct gtcttggatt ttgtgatcca gttgaaccaa caagagtaca
    10021 cagctgccat ttaccgaact ttaaaccaag tcaacaaccc gccgttttta gtgatgattg
    10081 aaaaattaga gagatacaga catggataaa caatacctac acgaaaagct ggatgccatg
    10141 cgccagaatt ttgttgaatc aacctaccac gaacgagcgg tgggcgtgct agaccaagcg
    10201 catatgagca aaaaaatgct taaaatcaag aaaaaattag ttgctcttga aatggaacgg
    10261 tgccagagaa aaattgagca caaagactgt tccaagattg accaaaaaat caaagagcag
    10321 aaggagatat ttgaatcctg ttgtaaaaaa gattaaggag gacgtgcgtg gaattactga
    10381 tttacctcat cctattttta ctggtcttga ttgtctcaag tacaaccaat aagctcctac
    10441 cctttttgcc tctccctttg gtgcagattc ttttgggaat tgtgattggt ctctttttac
    10501 ccaatactga ctttcacctt aatacggagt tgtttttggc actggttatc ggccccttgc
    10561 ttttccgaga ggctgaagaa gcagatgtta cggctatttt aaaacactgg cgaatcattg
    10621 tttatctcat atttccagtg atttttatct cgaccctgag tttgggtggc ttggcccatc
    10681 ttctttggtt cagccttccc ttggcagctt gcttggctgt tggggcagcc cttggtccta
    10741 cggacttggt ggcctttgcc tctctttcgg agcgttttag ctttcctaag cgcgtgtcca
    10801 atattcttaa gggcgaagga ctcttgaatg atgcttctgg tttggtggct tttcaggtag
    10861 ctttgacagc ttggacaact ggagcttttt ctctggggca agctagcagt tcactcatct
    10921 tttcaatcct aggcggtttt ttaattggat ttttaacagc catgaccaac cgcttcctcc
    10981 ataccttctt gctaagtgtg cgtgcaacgg atattgccag tgaactttta ttagaattga
    11041 gtttgcctct tgtgaccttc tttctggcag aagaagttca tgtttcaggt attattgccg
    11101 tcgtagttgc tggaatttta aaggcaagtc gtttcaagaa aatcacgctc ctcgaagccc
    11161 aagtggatac ggtgaccgag acggtctggc atacagtgac ctttatgctc aacggttctg
    11221 tctttgtgat tttagggatg gagctggaaa tgatagcaga acctatcttg accaatccaa
    11281 tctataatcc tctactttta ttgctatctc ttatcgccct tacctttgtc ctctttgtca
    11341 ttcgttttat tatgatctat ggctattatg cctatagaac ccgacgcctc aagaaaaagc
    11401 taaataagta tatgaaggac atgtttctct tgaccttttc aggtgttaag ggaacggtgt
    11461 cgattgctac gattctcttg ataccaagta atctagaaca ggagtatcct ctcttgcttt
    11521 tccttgttgc aggtgtgacg cttgttagct ttttaacagg tctcttggtc ttgcctcatc
    11581 tttctgatga agaggaagaa agcaaggatt atctcatgca tatcgccatt ttgaatgaag
    11641 taacgctaga gttggaaaaa gagttggaag acaccagaaa taaactttcc ctctatgcgg
    11701 ctattgacaa ttatcatgga cgtattgaaa atctcatttt aagccaagaa aaccaggatg
    11761 atcaagaaga ctgggctgct ttgaaactct tgattcttag tattgaaagt gatggtttgg
    11821 aacaggccta tgaagagggg aatattagca atcgtgctta ccgagtttac caacgttatc
    11881 tgaaaaatat agaacaagga atcaatcgta aacttgcctc aagactgacc tattattttc
    11941 ttgtttcctt gaggatttta cgttttctcc ttcatgaagt ttttactctt ggaaagacct
    12001 tccgtagctg gaaggacaag gagcaaagcc gtctccgtgc tcttgattat gaccaaattg
    12061 cagagctcta tcttgccaat acagagatga ttattgaaag tttggaaaac ctgaagggag
    12121 tctacagacg ctctttgatt agttttatgc aggagtctcg tcttcgagaa acagctatta
    12181 tcagcagtgg tgcctttgtc gaacgggtta tcaatcgtgt caaacccaac aatatcgatg
    12241 aaatgctgag aggctattat ctggagcgca agttgatttt cgaatacgaa gaaaaacgat
    12301 taattacaac taagtatgcc aagaaattac gacaaaatgt aaataactta gagaactatt
    12361 ccttgaagga agctgccaat accctgccgt atgatatggt ggaattggta agaagaaatt
    12421 agttaatact cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgga ttatatatgt
    12481 gactgacttc gtcagtttca tctacaacct caaagcaggg ctttgagcaa cctgcggcta
    12541 gcttcctagt ttgctctttg attttcattg agtataagat tataaatgaa ggagtatgac
    12601 atgaaaaagt ggtggaaaga gctgatagac aagcctttat taaaagcttt tttgcattat
    12661 tatcaagcat cagatagtga gttgaccagt gtcgcagtag cctactattg gttgatttcg
    12721 attttccccc tgcttttggt ggtggtcaat atcctcccct attttcagat tcctgtggga
    12781 gaatttctgt gctttatgaa agacgttctg cccccaagcc tctatgaagg tgtggaaaaa
    12841 atagccagag aagttttgac gcaaccttca acaggtttat tgagtttttc tgttttatcg
    12901 gcgttatgga gtttttcaaa atctatgaat ttcttgcaaa aagcttttaa caaggcttat
    12961 ggcgtcgaaa agagtcgtgg acttatttcc catcagatgc taagtctcct agtcagtttt
    13021 ggcttgcagc tcctctttgc gtttgccttg tttttgatct tatttggtca gatgattttg
    13081 gctttacttg ctcattattg gacaaaagat ggcattatct atcaggcttt acaagggttg
    13141 gcaggtcctc tgatttacgc cctcctcttt gctatcttgg tcatgcttta ttattttctt
    13201 cccaatatat ctaacaggaa aattagctat accctaccag gcagtgcctt tgttctctta
    13261 gtgattttag gtttactaac tctgttctct agttatctca attactatgt ccatcatttg
    13321 gtggatgtcc gaattttagg ttccgttctt cttgttgtta tgatgttttg gtttatcttg
    13381 attgctaaaa ttgttatcct aggcgcggtt atcaatgcca gtatgcagag tttgagagat
    13441 ccggtcttta aaaaagacta actatttatc cattacaaaa gcgcatagta tcaaaacttt
    13501 atagaacctt gatactatgc gcttttgtgc tttgacagtt ttttgtatag tttttgtcca
    13561 agatttctaa atcatctgat taaattccta ttttccccta tcattgtccc tgttttttcg
    13621 aaaatggaag gagtataata aatctatcaa taaaatgata ggaggaactt tatgggtcat
    13681 attttctttt ttctaagtgt ctttttggca gggattctat ccttcttttc tccttgtatc
    13741 ttacctttgt taccggtcta tacaggagtg ttactagatg ataaggatgg tgctcaggct
    13801 tctagcggca aattttcaat ctcagttact agtttattac gaactctggc ctttatagca
    13861 ggaatttcct ttatatttat tttgttgggc tatggagctg gttttttagg cgatttgctt
    13921 tatgcttctt ggttccaata tcttactggg gcaattatta tccttcttgg tttgcaccaa
    13981 atggagattc tacactttaa ggggctttat aaggaaaaga ggctacaact gcaaggacag
    14041 gggcaaaatg gtaagggcta tagtcaggca tttttattgg gcttgacctt tagttttgct
    14101 tggacgcctt gcgtggggcc ggttctgggg tctgttttgg ccttggcggc ttcaggtggt
    14161 tcaggagctt ggcagggagc tggtctcatg ttggtgtata cgctgggctt ggcgctacca
    14221 ttcttgcttc tagctctgac ctctagttat gttttgaaac atttccgaaa acttcatccc
    14281 tatctcggaa tcctcaaaaa agtgggtggt tttctcatta ttgtgatggg actcttggtt
    14341 ctgtttggaa atgcttcaat tttaagtcaa ttatttgaat aaaatggaaa ggaatatcaa
    14401 tatgaaaaaa tggcaaacat gtgttcttgg agcaggttcg ctcctttgtt tgacggcttg
    14461 ttcaggcaag tccgtgacta gtgaacacca aacgaaagat gaaatgaaga cggagcagac
    14521 agctagtaaa acaagcgcag ctaaagggaa agaggtggct gattttgaat tgatgggagt
    14581 agatggcaag acctaccgtt tatctgatta caagggcaag aaagtctatc tcaaattctg
    14641 ggcttcttgg tgttccatct gtctggctag tcttccagat acggatgaga ttgctaaaga
    14701 agctggtgat gactatgtgg tcttgacagt agtgtcacca ggacataagg gagagcaatc
    14761 tgaagcggac tttaagaatt ggtataaggg attggattat aaaaatctcc cagtcctagt
    14821 tgacccatca ggcaaacttt tggaaactta tggtgtccgt tcttacccaa cccaagcctt
    14881 tatagacaaa gaaggcaagc tggtcaaaac acatccagga ttcatggaaa aagatgcaat
    14941 tttgcaaact ttgaaggaat tagcctagga ggtgtcttat gaatgataag ttaaaaatct
    15001 tcttgttgct aggagtattt tttctagcca taaccggttt ctatgttcta ttgatacgaa
    15061 atgcagggca gacagatgcc tcgcaaattg aaaaggcggc agttagccaa ggaggaaaag
    15121 cagtgaaaaa aacagaaatt agtgaagacg cagacttgca cgaaatttat ctagctggag
    15181 gttgtttctg gggagtggag gaatatttct cacgcgttcc cggggtgacg gatgccgttt
    15241 caggctatgc aaatggtaga ggagaaacaa ccaagtacga attaattaac caaacaggtc
    15301 atgcagaaac cgtccatgtc acctatgatg ccaagcaaat ttctctcaag gaaatcctgc
    15361 ttcactattt ccgcattatc aatccaacca gcaaaaataa acaaggaaat gatgtgggga
    15421 cccagtaccg tactggtgtt tattacacag atgacaagga tttggaagtg attaaccaag
    15481 tctttgatga ggtggctaag aaatacgatc aacctctagc agttgaaaag gaaaacttga
    15541 agaattttgt ggtggctgag gattaccatc aagactatct caagaaaaat ccaaatggtt
    15601 actgccatat caatgttaat caggcggcct atcctgtcat tgatgccagc aaatatccaa
    15661 aaccaagtga tgaggaattg aaaaagaccc tgtcacctga ggagtatgca gttacccagg
    15721 aaaatcaaac agaacgagct ttctcaaacc gttactggga taaatttgaa tccggtatct
    15781 atgtggatat agcaactggg gaacctctct tttcatcaaa agacaaattt gagtctggtt
    15841 gtggctggcc tagttttacc caacccatca gtccagatgt tgtcacctac aaggaagata
    15901 agtcctacaa tatgacgcgt atggaagtgc ggagccgagt aggagattct caccttgggc
    15961 atgtctttac ggatggtcca caggacaagg gcggcttacg ttactgtatc aatagcctct
    16021 ctatccgctt tattcccaaa gaccaaatgg aagaaaaagg ctacgcttat ttactagatt
    16081 atgttgatta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg
    16141 tagggttact gacttcgtca gttttatcta caacctcaaa gctatacttt gagctgactt
    16201 cgtcagtttt atctacaacc tcaaagcggt actttgagaa acctgtggct atcttcctag
    16261 tttacttttt gatttttatt gagtataaga ggtctttcct aagcagttag aggagaattt
    16321 tgctatactg atactagtaa gtggtaaagg agcagagcat gacctacaca atcttaatcg
    16381 tagaagatga atatctggta agacaaggtt tgactaaact ggtcaatgta gcagcctacg
    16441 atatggaaat catcggtcag gctgaaaatg gaaggcaggc ttgggaattg atccaaaagc
    16501 aggtgccaga tatcatttta accgatatca acatgcctca tctaaatggc atccagttgg
    16561 ccagtctggt acgagaaacc tatcctcagg ttcatttggt ctttttaaca ggttacgatg
    16621 attttgatta tgccttgtct gctgtcaaac taggtgtgga cgactacctg ctcaaaccct
    16681 tttctcgtca ggatattgag gaaatgttgg ggaaaatcaa acaaaaacta gacaaggaag
    16741 agaaagaaga gcagttacaa gatttattga ctaataggtt tgaaggaaac atggcccaga
    16801 aaatccagtc tcatctggct gatagccaat ttagtttaaa gtctttagcc agtgacttag
    16861 gttttagtcc gacctatctg agttccttga ttaagaaaga gttgggcttg ccttttcagg
    16921 attatctggt gagagaacgt gttaaacaag ccaagctctt gcttttaact acagatctga
    16981 agatttatga gatcgcagag aaggttggtt ttgaagatat gaactatttt acccaacgtt
    17041 ttaagcagat tgcaggtgtg acacctcgtc agtttaagaa gggagaagac cgatgaagcg
    17101 ttcttctctt ttagttagaa tggttatttc catctttctg gtctttctca ttctcctagc
    17161 tctggttgga actttctact atcaatcaag ttcttcagcc attgaggcca ccattgaggg
    17221 caacagccaa acgaccatca gccagactag ccactttatt cagtcttata tcaaaaaact
    17281 agaaaccacc tcgactggtt tgacccagca gacggatgtt ctggcctatg ctgagaatcc
    17341 cagtcaagac aaggtcgagg gaatccgaga tttgtttttg accatcttga agtcagataa
    17401 ggacttgaaa actgttgtgc tggtgaccaa atctggtcag gtcatttcta cagatgacag
    17461 tgtgcagatg aaaacttcct ctgatatgat ggctgaggat tggtaccaaa aggccattca
    17521 tcagggagct atgcctgttt tgactccagc tcgtaaatca gatagtcagt gggtcatttc
    17581 tgtcactcaa gaacttgttg atgcaaaggg agccaatctt ggtgtgcttc gtttggatat
    17641 ttcttatgaa actctggaag cctatctcaa tcaactccag ttggggcagc agggctttgc
    17701 cttcattatc aatgaaaacc atgaatttgt ctaccatcct caacacacag tttatagttc
    17761 gtctagcaaa atggaggcta tgaaacccta catcgataca ggtcagggtt atactcctgg
    17821 tcacaaatcc tacgtcagtc aagagaagat tgcaggaact gattggacgg tgcttggcgt
    17881 gtcatcattg gaaaagttag accaggttcg gagtcagctc ttgtggacct tgcttggggc
    17941 cagtgtcaca tctcttcttg tctgtctctg cttagtgtgg ttcagtctta aacgctggat
    18001 tgctcctttg aaggatttga gagaaaccat gttggaaatt gcttctggtg ctcaaaatct
    18061 tcgtgccaag gaagttggtg cctatgaact gagagaagta actcgccaat ttaatgctat
    18121 gttggatcag attgatcagt tgatggtagc tattcgtagc caggaagaaa cgacccgtca
    18181 gtaccaactt caagcccttt cgagccagat taatccacat ttcctctata acactttgga
    18241 caccatcatc tggatggctg aatttcatga tagtcagcga gtggtgcagg tgaccaagtc
    18301 cttggcaacc tatttccgct tggcgctcaa tcaaggcaag gacttgattt gtctctctga
    18361 cgaaatcaat catgtccgcc agtatctctt tatccagaaa caacgctatg gagataagct
    18421 ggaatacgaa attaatgaaa atgttgcctt tgataattta gtcttaccca agctggtcct
    18481 acaacccctt gtagaaaatg ctctttacca tggcattaag gaaaaggaag gtcagggcca
    18541 tattaaactt tctgtccaga aacaggattc gggattggtc atccgtattg aggatgatgg
    18601 cgttggcttc caagatgctg gtgatagtag tcaaagtcaa ctcaaacgtg ggggagttgg
    18661 tcttcaaaat gtcgatcaac ggctcaaact tcattttgga gccaattacc atatgaagat
    18721 tgattctaga ccccaaaaag ggacgaaagt tgaaatatat ataaatagaa tagaaactag
    18781 ctaactccca gtcaattctg ggagttttat gcgtaattgg gcaaactttc taggttgtct
    18841 atcttgctaa aaacaagaaa aaacggtatg atagaagagt gacaacagag gtaacagaga
    18901 tgaacgagac acagattcaa agagaaacac gtcaggtagt ggaagatgtt ttagaaaaga
    18961 ccaatttgaa gcagggggcc ctttttgttt taggtctatc ttctagtgag gtgctaggtg
    19021 gtcatattgg gaaagaatcc agtcaagaga ttggtgagct gatagtggag actatcttgg
    19081 atattctagg tagtagaggt atccatttag cagttcaagg ttgtgagcat gtcaatcggg
    19141 ctctcgttgt tgaacgtcag gtggcagagc agtttggtct ggaaattgtc agtgtccatc
    19201 ctactcttca tgcaggaggt tcggggcagt tggcagcctt taagtttatg caggatccag
    19261 ttgaggttga atttatcaag gctcatgctg gattggatat cggagacact gcaattggca
    19321 tgcatgtcaa gcatgttcag gttccgattc gccctatttt gagagagatt ggtcatgccc
    19381 atgtaacggc actggctagt cgtccaaaat taatcggagg tgcgcgtgcg cactatccgc
    19441 aagatgctat tagaaagacc tgagaatgaa taaaggagat aaaagtgttt aaaaaagacc
    19501 gtttttcaat tcgtaagatt aagggagttg taggctctgt atttcttgga agccttttga
    19561 tggctccttc tgtagtggac gcagccacct atcactatgt aaataaagag attatttcac
    19621 aagaagctaa agatttaatt cagacaggaa agcctgacag gaatgaagtt gtatatggtt
    19681 tggtgtatca aaaagatcag ttgcctcaaa cagggacaga agcatctgtt ttgacagctt
    19741 ttggttatct atcaggtgac atcctcaaaa cgcttggctt ggacactgtt ttagaagaaa
    19801 cctcagcaaa acctggagag gtgactgtag tcgaagttga gactcctcaa tcaacaacaa
    19861 atcaggagca agctaggaca gaaaaccaag tagtagagac agaggaagct ctaaaagaag
    19921 aagcacctaa aacagaagaa agtccaaagg aagaaccaaa atcggagata aaacctactg
    19981 acgacaccct tcctaaagta gaagagggga aagaagattc agcagaacca gctccagttg
    20041 aagaagtagg tggagaagtt gagtcaaaac cagaggaaaa agtagcagtt aagccagaaa
    20101 gtcaaccatc agacaaacca acagaggaac caaaagttga acaagtaggt gaaccagtcg
    20161 agccaagtga agacgaacag gcaccaacgg caccagttga gccagaaaag caaccagaag
    20221 ctcctgaaga agagaaggct gtagaggaaa caccgaaacc agaagataaa ataaagggta
    20281 ttggtactaa agaaccagtt gataaaagtg agttaaataa tcaaattgat aaagctagtt
    20341 cagtttctcc tactgattat tctacagcaa gttacaatga tcttggacct gttttagaaa
    20401 ctgcaaaagg tgtctatgct tcagagcctg taaaacagcc tgaggtaaat agtgagacaa
    20461 ataaacttaa aacggctatt gacgctctaa acgttgataa aactgaatta aacaatacga
    20521 ttgcagatgc aaaaacaaag gtaaaagaac attacagtga tagaagttgg caaaacctcc
    20581 aaactggggt tacagaagct gaaaaagttg cagctaatac agatgctaaa caaagtgaag
    20641 taaattctga gactgcaagt cttaaaacag caatttctag attaaataca gataaagttg
    20701 aactcgaaaa tcaacttaag attgcacagg gaaaaactga aactgatttc agcatggaat
    20761 cttggactgt tttgagtaca gctaaaaata aagctcagga agtaaaagat aacggaacag
    20821 cgacccaaga acaaataaac gaagcagaga aaagtttgaa aacagccctt gctgacttat
    20881 ctgttgacaa aacagctttg ggaagcgcta tcgatacagc tactaagaaa aataaagaga
    20941 attatacaaa tcaaacatgg gcagagttgg aaacagcgct tacagcagca aaatctgtaa
    21001 atactaacga gtctaaacaa agcgacgtta acgaagctgc tgaaaaatta actgcaacta
    21061 tggaaaaatt ggttgaattg tctgaaaagc cacgtttgac tctatctatt gaaaagagag
    21121 acatagatcg aaaagctacg gtcacgtata ctttggaaaa tccagcaaat actcaaataa
    21181 aatcaataac tgcaactctg aaaaaaggag aggaagttgt taaggatttt gttttaacag
    21241 aagaaaatct taaaactaat catttaactg ctttgtttga aaagctagac tactacaaag
    21301 aatacactct atctacggat atggtatata acagaggaaa tgatgatgag acggagagta
    21361 tttcagaaga actaattcag ctgaatctta agaaactaga attgaaagat atccagacag
    21421 tctctctgat gaaatttgaa aatggtcaag aaagtcaggt tacacacttg tctgataaac
    21481 cgaccgattt gagtaaatta tacttaaaag ttacatcatc aacgtctaag gatgctgtgt
    21541 tagccgttag ctctatagaa gaagagatta ttgaaaataa aaaaatcttt aaaattcatg
    21601 cagatacacc agaattagta gttcgtaaga aagatggcag tctttctaag ggatttgact
    21661 attacatgga gagagttatc cctcatgatg gtgatattta ttatgatttt aaagacttga
    21721 tttcagctat gacatctaat cctacaggta cgtttatttt agggagagat atttcctcgc
    21781 gtaatgtaaa accggatgga aatggaaaat cttatatcaa aggtgaattt aaaggaaaac
    21841 tacttggtac aaatgataat gttagacact cgatctttga cttagaatat ccattgtttg
    21901 atactataaa atcaggagtc gtgaaagata ttgatttcaa gcatgtcaat atggtattcc
    21961 cagatagcaa tcaaggagat aacgtagcaa caattgcaag agtaatcaaa gataagacaa
    22021 aaattgagaa tgttaatgtt gaaggttatc tcgagggtcg agatcatgta gctggtttag
    22081 ttaataatct tgaaggtaat tccgaaattg aaaatgtatc ctttacagga aagattaagt
    22141 ctaaaggtgg taattctatt acagcaggta ttgcaggtag aaatatctta tctcgtgtta
    22201 aacgtgccta tgtaaatgca gatatagagg tacatcgatc atctaattct agcatgcttg
    22261 ttgcagtaaa tggaataaac gcagatgcaa gtggtggttg gggaacctgg ggtagactta
    22321 ctgagtcagt tgctaaagga acattagaaa ccaagcaagg agggcaagct ggaggagcct
    22381 cttctacagt ctggccttat ggagcaattg ataatgttgt cagctatgca aaagtgacca
    22441 aagggaaaga attatttgga tcagatggtg atttaaatta tgattggttt atgaaaaaaa
    22501 ttagtaatat ttttggtgtt caaggcatca gctcaggcga ttcgggtagt gattctaaat
    22561 ttacgcgtat ttctgaggaa gaagcaaatc aaaaagttgc ttcttataat attacagctc
    22621 ccaacttgat gagtgactca agtcttttgg tagacagact caatgaaagt tggaaaaata
    22681 ctgatcaatt tgaaagtata caggattatc aagcccaaaa ccaattaatt taccagaact
    22741 tgactaaatt tactccttat tacaataaag aatttattgt tcatgaagga aatgctctca
    22801 ctcctgaaca ggaaatattg aagactaaga aaatcaaatc gattgtaggg ttgaaaggaa
    22861 cagagttcgt tgtagatggc agtgatattg atactatcat gcttcatttt gaggatggta
    22921 gtcagaagcg atataaggta acctctactg gtaagttttc gataactaat ttaccggaat
    22981 atcaggttga agatttaaat gtggtgtata catcagaaca tatagtacat ccactagatt
    23041 cgtcactgat aaataacctt gttgaagaat tgaaaaaagt agaattgtat acagagtcta
    23101 cttaccaagt tctaggtatt gataaagata atgctaacaa attgaataga actaagagat
    23161 tattcttaga tgagtcatta gatgctgtta aaacacaatt gcctaccttt gtaaaaacaa
    23221 tgtttgaaaa tgagtggttg cacatcaatg gagaatctag tggagctgtc gctgctttac
    23281 gacaaaaaat catggataat aagacggcta tattgcttgc tttgacttat atcaatcgtt
    23341 attatgatgt taaattctca gactataata ttaaaaaact gatgctcttt aaaccaactt
    23401 tccatggtga aaagattgat ctattagatc gattaatccg actaggatca tctggagaaa
    23461 atagattaaa aggttcagaa aatgcagaaa cgttcaagca gttatttgct agcgaaacga
    23521 agcagaagga tttggtgact tatttagact acaatcgctc tttgttgact aattatcaaa
    23581 caacagggga atggtttaaa gaaacgacta aggattatat tcaatttgag gaacgtccat
    23641 ccttagtaga ggagattaaa gatgccaaat atcgagttta cgataattta accgcaccgt
    23701 attatcaagg atatatccta cctctcttaa cgttgaagaa tactcattta gcaattttat
    23761 cgaactatag tactatgaca tttgtcagcc gtgaaaaacg ccctaattgg aaaaatgaag
    23821 attttgataa gtgggtgaaa tatgtagcaa ctgctcatag aaaccatgtt gatacttggt
    23881 ataagatttt acctgataat atcaaaggta aaatggttaa agagaatgtt actgctgttt
    23941 gggaaggatt gagcatccca ggaagtgaat gggtagacca gaatgctgta gatagaaaag
    24001 gaagagatta cgccccagct cgagaattct ttaatcttgt aggtggtcct atgggaggtt
    24061 ggtatgctta ccatggttat ggtgcgcatg ctggaggaag aaatcgggtt aattatgagg
    24121 tgtttgatgt attaagtgag tatggtatat cggtctttac tcatgaattg actcacgtta
    24181 atgatacttg gatttactta ggagggtatg gtagacgtga aaatatggga cctgaagctt
    24241 atgctcaggg attattccag tctcctgttc caggtcagcc gggttggggg gcgcttggtc
    24301 tcaatatggc atttgaaaga aaaaatgatg gtgatctaat ttataatgcc tccccaactc
    24361 aatttgaaaa taggaaagag ttagatagtt atatgaaaaa ttataacgat actctcatga
    24421 tggtggacta cttggaagga gatgcagtta tctctaaagg taaagaagct attactaaat
    24481 ggtttaaaaa agttgaacct aaggttgtta gtcaaacagc tcagtatgat acggttcgtc
    24541 agttgactgc tgaagagaaa gaaaaacttt ctgtttctag tgttgatgat ttagttgatc
    24601 aaggattaat gtcagacaga gctgtaggaa ataataccta taatccagct gattttgaaa
    24661 caagttatat tgcgatagat tatatgactg gtatttatgg tggaggaaag aatagtgtag
    24721 gttcaccagg tgccttgatg tttaaacata atactttccg tatgtggggc tactatggtt
    24781 ttgaagaagg tgttctagga tatgcttcta ataaatttaa gcaagcatct agagatgaag
    24841 gtcatgctgg acttagtgat aactttatta tttctaagat ttccaaaggg gaatttttaa
    24901 ctatggaagc atttaaaaag ggatatttta agaaagtagt agaagagtta aaaactaaag
    24961 gtattcgtcc tgttactatc aatcaaaaaa catatagcac ctttgaagaa ttgcaagaag
    25021 gatttaagca ggctgttgaa agagatttga agaaaaatca gttagatgag agggaaacaa
    25081 gaaacttcaa attccaagtt ttcagacaac ttcttcaaca aacagatagc tttaaaactt
    25141 ctatctttag ataatcgaaa tctctcatct gctttgcgga tgagggtttt cttattttat
    25201 gctatactta aagtatgcat agaaaaacag tgattgattt taggactttg ggggagagat
    25261 atacctttac ccagcctatt aaagagttga aaacgagaaa tgtagaagaa gtggcagatt
    25321 tgctggcaca agtggaaagc taccaagagc aaggttatta tgtggtgggg tatgtcagct
    25381 acgaggctgc acctgctttt gaggaaaaat tagcagttca caaggatccc ctactgggag
    25441 agtacctgct ttattttact gttcatgata gcgtagaaac ttcccctatt cctctgactt
    25501 atgaggatat tgatttgccc tcaaattggc aggaagtaac gtctgcagca gactatgaaa
    25561 aggccattgc ccaaattcac catcatttac ggcagggaga tacctatcag gtcaactaca
    25621 ccgtccaact taagcaaaag ttaaatgcca atccttttgc catctacaat cgtatggtgg
    25681 tagagcagga ggcgggctac aatgcctatg tggaacatga cgagatggca gtgatttcca
    25741 tgagcccaga gctctttttt gagcaaaatg atcgcgagtt gacaacacga ccaatgaagg
    25801 ggacgactca gcgtggggta actgaccaag aagatcttga acaggccagt tggttggaac
    25861 aggatcccaa aaatcgctct gaaaatatga tgattgtgga cctcttgcgc aatgatatga
    25921 accgtatttc tgaagttggg agcgagcacg tggagcgtct gtgtcaggta gagcagtatt
    25981 caactgtttg gcagatgact tcgaccatca agagtcagtt gcgagaggat gtggaccttg
    26041 ttgaaatctt ccactcactc tttccttgtg gttccataac gggtgcaccg aaaattgcga
    26101 caatggagat tatcaaggac ttggagcctc aaccgcgtgg agtctactgt ggaacgattg
    26161 gtctcttgct tccaaatgga cgacggattt ttaatgtggc cattcgtacc attcaacttc
    26221 acaaaggtca agccatttat ggagttggcg gagggattac ttgggatagc acttgggaat
    26281 ctgaataccg tgaagttcat caaaaggcag cagttctcta tcgtaaacaa gctcgcttcc
    26341 aattaattac aactggaaaa atcagccaaa aacaactgat gtttgaagaa caacatctgg
    26401 aaaggctgag aaaagctagt cggtattttg cttatccttt tgacccagaa attttgagac
    26461 aaaagattga agcagagtgt cagacttgtg atgctaatca agattaccgc ttgcgaattt
    26521 ctctcagcaa gtctggagag atagaattca gtcgtcaaat cttaacacct cttagtccaa
    26581 gtttttgtca ggccaaactt tgtctgcaag aagcgaattt gaatcaatcc tttacctatt
    26641 ttaaaacgac ttacagaccg catttgagca tagaggaaca agagaagatt taccataatg
    26701 gaaaaggaga acttcttgaa acctctatcg gaaatttggt tctgaaaatc gatgggaaac
    26761 tctacacacc gcctatccga cttggaatct tgccaggaat ttatcaccag tatttactag
    26821 aaacaggaca ggtagaagag aaagtcttga ccttggcaga ttttgcccaa gcagaagcta
    26881 tttacggctg taatgcagtg agaggcttgt atgagttgca agtgatagga ttgtgagttt
    26941 tagctattaa tagtttttaa tatatattaa aatatattgg tgtttacttt tgatgagata
    27001 tcatatttgt gttataatgt aaccgtaaac aatagaaatt taaaggagat ttttgatgaa
    27061 atcaaaaata gttttgggag cttcgctagc gatcgcaaca cttagcttag tatctcttgt
    27121 cgagattgaa ggactctcac cgtttttaat agagaatgtt agcgcaaata ctcattctgc
    27181 aaataaagtt attaatcata aagtaagtat ttacttagaa aatgctgatg aaggtaaagg
    27241 tctaactgta aacttttcaa cagacagtgt tagtccaaat ttatttgatg agttcgaaaa
    27301 aaagagtgga attacaataa ctactatgtt agtgaatgct aagacaggtg aagttgtaga
    27361 aaaaagatta acaccaagcg tttttttacg ttccaatgat ttaaccagtg ggaccatatc
    27421 ttcgtttatt tttagtgaat atcctgatgg agaatataaa tatgttgtgt ctaaaggaga
    27481 ctttatcgat cctaagactc agtttaaaca tcagtataga ggagaatctc cagtttttag
    27541 aatacgtaat cgaaaatatg tggagttggg aacaactgat aaaaaattag atgaaagacg
    27601 tgataatagc gtttataaag atggggttgt tgaacacaag gtaaatttaa gtttaacaag
    27661 ttatcaagga ggtaatggag ttactgcaat tttttcaact gatagtgtta actctaactt
    27721 acttaattct tttggtgaaa aggctaaaaa agttttgatt aggtctaagt taattaatgt
    27781 taagactggc gaggttattg atgaaacgtt ttctccaaaa gtaagtttaa cctctaaaat
    27841 tttgaaaagt ggttctacag cagtatttta ttttatagat ttaactgatg gcgaatataa
    27901 atatgttgcg tatgaaagcc agcaatatac tgatcctcaa acaacattga ctcatcagta
    27961 tagaggagaa tctcctattt ttagtatcaa agatggaaaa ttttctggtt tagtatctgc
    28021 ctcaaaacca gacgaaaacc cgaagccaac gccaaaaccg gatgagaaac caaaaccttc
    28081 agcaccacaa caggagaaaa caaaacctac agttcagtct ggttgggtag gttcatctta
    28141 ttatcaaaat ggtaaaaaag tcactagtaa gtggattttt gataaaaaat ataattcata
    28201 cttctatcta gatgcatcag gaaattatgt tcaaaacgct tgggtaggta actattatct
    28261 caaatcaggc ggatacatgg ctaaaagtga gtggatttat gataagaact atggttcata
    28321 ctactatcta acatcagaag gcagctacgc tcgtaacact tggtcaggca attattattt
    28381 gaagtcaaat ggtaagatgg ctaaaagtga gtgggtatac gatagtaact acaaatcata
    28441 ttactacttg acatcagaag gaagttatgc tcgtaacact tgggtaggca attactatct
    28501 taaatcaaac ggtaaaatgg ctgttaatga acgtacacca gatggttatc gtgtagatgg
    28561 ttcaggtaaa tgggttaaat aagaaactaa aaaataggat aataggtaat atcagatgaa
    28621 taaaaaaaat tattttcatg tgctttagca ttttcaacga ttggaacatt gtcaacgatt
    28681 gataaatcag gagttcttcc tttttcagcg caaacggtgc aggctgacat acaaacgctt
    28741 accccaatgg caaaatatta tattattctt ccgaaggatg cagaaatata taaaacttgg
    28801 agaggtactg tcaatattcc cattattgat gctacaaaaa ctactccaga attatcttat
    28861 ttcaaagaag atcatcgcaa ctatatagct aatgagaata agtctggtgc caattacata
    28921 gagtggaagg gaacagtaga agagtttaaa gaggccatta agaagttgac tgataaaaaa
    28981 tcaactactg caacaccaaa aaaggatgaa aagccaacac caaaaccgga tgagaagcca
    29041 aaaccaacac ctacagttca gtctggttgg gtaggttcat cctattatca agatggtaaa
    29101 aaagtcatta gtaagtggat ttttgataaa aaatataatt catacttcta tctagatgca
    29161 tcaggaaatt atgttcaaaa cgcttgggta ggtaactatt atcttaaatc aggcggatac
    29221 atggctaaag gtgaatgggt ttatgatgcc acctatcaag cttggtatta tttgacatca
    29281 gatggttctt atgcttacag tacatggcaa ggaaattact atctaaaatc ggatggtaaa
    29341 atggctgtca atgaatgggt tgatggtgga cgttattatg ttggcgctga cggagtttgg
    29401 aaggaaggtc aagcaagtac agcttcttct agtaatgata gcaatagtga atattctgct
    29461 gctttaggaa aggcaaaaag ttataattcg ttattccaca tgtcaaaaaa aaacgtatgt
    29521 atagacaatt aacttctgat tttgataaat tttcaaatga tgcagctcaa tatgccattg
    29581 atcattagat gattaacaga tactggtagg cgaaaaaatt ccttataatc catttacgca
    29641 aacctttgca taagtgaatg gattttgata tactagtact gtaagcattt tcaattacta
    29701 cacaatgaaa aaggagaata tgatgagtca aaagattatt gggattgacc ttggtggaac
    29761 ttctatcaaa tttgcaatct taacaacagc aggagaaatc caaggaaaat ggtcaatcaa
    29821 gaccaacatt ttggatgagg gaagtcatat cgttgatgat atgattgagt ctattcaaca
    29881 tcgtttggac ttgcttggat tggcagcagc ggacttccaa ggcattggaa tgggatcacc
    29941 aggtgtggtt gaccgtgaca aagggactgt tatcggtgcc tacaacttga actggaaaac
    30001 ccttcaacca atcaaacaaa agattgaaaa agctttgggc attccatttt tcatcgataa
    30061 tgatgccaac gtagcagctc ttggtgagcg ctggatgggt gctggagata accaaccaga
    30121 cgttgtcttt atgacactcg gtactggtgt tggtggcggt atcgtcgcag aaggcaaatt
    30181 gcttcacggt gttgctggtg cagcaggtga gcttggtcac atcactgttg actttgacca
    30241 gccaatctca tgtacttgcg gtaagaaagg ctgccttgag acagttgctt cagcaacagg
    30301 gattgtcaac ttgactcgtc gctatgccga tgaatacgaa ggcgatgcag ccttgaaacg
    30361 cttgattgat aatggagaag aagtaactgc taagactgtc tttgatctcg caaaagaagg
    30421 agacgacctt gctttgattg tttaccgtaa cttctcacgt tacttgggaa tcgcttgtgc
    30481 taacattggt tcaatcctaa acccatcaac aatcgtcatc ggtggtggtg tgtcagctgc
    30541 gggagaattc cttctacaag gtgttcaaaa agtttacgat gaaaatagtt tcccacaagt
    30601 acgcacatct actaaattag ctcttgcaac tctaggaaat gacgctggag ttatcggagc
    30661 agcatcactt gtattgcaat aagataatga gagaggaaaa tgctaaccta gagttagtaa
    30721 tgttcctctt ttatttttta tgctatacta gttaggacaa tgaatgaggt gagagtaaat
    30781 gacaaaagca gatacgattt ttaaagagaa tattgaatgc attctaaagg aaggtgtttt
    30841 ctcagagcaa gctcgtccta agtacaagga tgggactgtt gctaattcca agtatgtaac
    30901 tggtgccttt tccgagtacg acttatctaa aggagaattt cctatcacaa ccttgcgtcc
    30961 cattgcaatc aaatccgcca tcaaagaagt tctctggatt taccaagacc agtcaaatag
    31021 cttagaagtg cttaatgaca agtacaatgt tcactactgg aatgactggg aagttggaga
    31081 cacgggaacc attggtgagc gctatggtgc cgttgttaag aaacacgaca ttatcaataa
    31141 gcttctcaaa cagttggaaa ccaatccttg gaatcgccgc aatattattt cgctctggga
    31201 ttaccaagct ttcgaagaaa cagatgggct gctcccgtgc gcctttcaga ccatgtttga
    31261 tgtccggcgt gttgatgggg aaatctatct ggatgcgacc ttgacccagc gctccaatga
    31321 tatgctggtg gcccaccaca tcaacgctat gcagtatgtg gctttacaga tgatgattgc
    31381 caaacatttt ggttggaagg ttgggaagtt cttctatttc atcaacaacc tccatatcta
    31441 tgataatcaa tttgaacaag ctcaggaatt gctccgtcgg gagccgtcaa actgccaacc
    31501 acgcttggtt ttaaatgttc ctgatgggac taatttcttt gatatcaaag cagaagattt
    31561 tgagttggtg gattatgacc ctgttaagcc acagttgaag tttgacctag ctatttaaaa
    31621 gaatagaaaa aagaagttga gaataatccc aacttctttt gtttcttaac gtgatacgcg
    31681 gcgacgagct gcttttttac gattttcttc gatgaaagct gctttttgct cttctggttc
    31741 gattactttc tttttaaatg cgtatactgc acctgcaacg gcagcgacag ttcctgcgac
    31801 acctgttaca agacctttag cgaatccttt agccatgagt cttcctcctt tatattctca
    31861 atcagccagc ctcctcaaga ggtcacattt ttctgactga cctttttgtg ttataataat
    31921 agtaacgaaa aaatgggaat ttttcaagga aaaaagatga aaacaaaaat aattgtgatt
    31981 gttggaccga ctgctgttgg aaagacggct ctagctattg aagttgcaaa gcgctttaat
    32041 ggcgaagtgg ttagtggaga tagccagcaa gtctatcgag gacttgatat tgggacggcc
    32101 aaggctagtc cagaagagca ggcagctgtt cctcatcatt taatcgatgt tagagagata
    32161 accgagtctt actcggcttt tgattttgtt tcagaagcta agatgactat tgaggatatt
    32221 cagagccgtg gcaagctagc cattatcgcc ggtgggactg gactttatat ccagagcttg
    32281 ctagaaggtt accacctagg tggggagact cctcatgagg agattttagc ttatcgagct
    32341 agtttggagc catattcaga tgaggaatta gcccatttgg tggagcaagc aggccttgag
    32401 attccccagt ttaatcgtcg tcgtgctatg cgtgccttag aaattgccca ttttggtcag
    32461 gatttggaaa atcaagagat cttgtatgaa ccgctgatta tctgcttgga tgatgaacgt
    32521 agtcaacttt atgagcgtat caaccaccga gtggatttga tgtttgaggc tgggcttttg
    32581 gatgaggcca agtggctgtt tgaccattcc cctaatgtac aggctgctaa aggaattggc
    32641 tataaggaac tctttcctta tttccgtgga gagcagacct ttgaggaagc tcgtgagagt
    32701 cttaaacagg cgacccgtcg ttttgccaag cgtcagctga cctggttccg taatcgcatg
    32761 caggtcacct tttatcagat tggagaatct ggtgtacaag accgcatttt aagccagata
    32821 gaggagtttt tagatgattg aaacggagaa aaaagaggag cgagtcctgc tgattggtgt
    32881 ggaattgcag ggtatggaca gttttgacct ctccatggaa gaattggcta gtttagcgaa
    32941 aacggcaggg gcagtcgttg tagatagcta cagacaaaaa cgtgaaaaat atgattccaa
    33001 gaccttcgtc ggctctggta agttggaaga gattgcgctt atggtggatg cagaagaaat
    33061 cactactgtc atcgtcaaca atcgtctgac cccaaggcag aatgtcaatc tagaggaagt
    33121 tctcggtgtt aaggtcattg accgtatgca attgattttg gatatctttg ccatgcgggc
    33181 tcgaagccat gaagggaagc tccaagtcca cctagcccaa ctcaaatacc tcttgcctcg
    33241 cttggttggt caggggatta tgctcagccg tcaggcaggg ggaattggtt cccgtggtcc
    33301 tggtgaaagc caactggagc tgaaccgtcg tagcgttcgc aatcaaatca cggatatcga
    33361 gcgccagctt aaggtggttg agaaaaatcg tgcgactgtc agagaaaaac gtttggagtc
    33421 tagcactttt aagattggtt tgattggtta tactaatgct gggaaatcaa ctatcatgaa
    33481 catcttgacc agtaagaccc agtatgaagc agatgagctc tttgcgactc tggatgcgac
    33541 aaccaagagt attcatctgg gaggcaatct ccaagtaact ttgacagata ccgttggctt
    33601 tatccaagat ttgccgacag agttggtgtc cagtttcaag tcaaccttgg aagaaagcaa
    33661 gcatgtggac cttctggttc atgttatcga tgctagcaat ccttaccacg aggagcatga
    33721 aaaaacggtt ctctccatca tgaaagacct ggacatggaa gatattcctc acttgacgct
    33781 ttataataaa gcggatttgg tggaggattt cacgcctacc caaacgccat ataccctcat
    33841 ttctgccaag tctgaggaca gtcgtgaaaa cttgcaagca ttattgctag ataagattaa
    33901 ggaaattttt gaagcattta ccctgcgagt gcctttttca aagtcctaca agattcatga
    33961 tttagagagt gttgcaattc tggaagaacg tgattatcag gaagacggcg aagtgattac
    34021 aggctacatt tccgagaaaa ataaatggag gttagaagaa ttttatgact gatattaaaa
    34081 ctttggctct aaagtatgga ggttatacaa gtctggacaa ggtctatctg gatcagcttc
    34141 tagctggtaa aacagatcag gagcagttaa cacttattac acctccgcca agtgttgtta
    34201 atgcctactt tgcagaactc tatcagaaaa agagtcctca ggctgcgacg gattattttg
    34261 cagaactcag tcaggaactg aatctttaca atactgagcc aagtttcacc ctagaaaata
    34321 agccttttat tcggcttaat ctgtctggta aatcctttgg tttttgttat gagagtgacg
    34381 gccttggtcg gattttcttt gaaaatgagg agaaaatctc ggatgacttg ctctttgaaa
    34441 ttgcgcaaat tttcccccat caattagtct ttgaagagtc tggtaagatt tacatgaagc
    34501 ctgtcgagga cgaggaagtt atcagcctag aaagtctcac agctttgact gatttggaaa
    34561 gattggctga tggtcgaaaa cgtctcaaag gctacagcca agaggattta ttgcaagaag
    34621 ctgctgcttt ttctggcaaa cgctatttcc gatcggaaaa ccgcacagcc atgttatata
    34681 ttgattaatt agaaagtatc gaatggatat tcaattttta ggaacggggg ctggtcagcc
    34741 ctctaaagcc cgcaacgttt caagtctcgc cctgaaactt ttggatgaga ttaacgaagt
    34801 ttggctcttt gactgtggag aaggtacgca aaatcgcatt ctggaaacca caattcgacc
    34861 acgtaaggtc agcaaaatct ttattaccca tctgcatgga gaccacattt ttggtttgcc
    34921 aggtttcctt tctagccgtg cctttcaggc caatgaagag cagacagatt tggaaatcta
    34981 cggacctcaa ggaatcaagt catttgtctt aaccagcctt cgtgtgtcag gttctcgtct
    35041 gccctaccgc attcatttcc atgagtttga ccaagattct ctgggtaaaa ttcttgaaac
    35101 cgataaattc actgtgtatg cagaggagct ggaccacact attttctgtg ttggctatcg
    35161 tgtcatgcaa aaggatctag aagggacgct ggatgctgaa aaactcaagg ctgctggtgt
    35221 tccgttcggc ccgctttttg gtaaaatcaa aaacggccag gatcttgttt tggaagacgg
    35281 aactgaaatc aaggcagcag actatatctc agcgccacgt ccaggtaaga ttatcactat
    35341 tttaggagac actcgaaaaa cgggtgccag tgtgcgtctg gctgtcaatg cagatgtcct
    35401 agttcatgag tccacttatg gcaagggtga tgaaaaaatt gctcgtaacc atggtcactc
    35461 aactaatatg caagctgcac aagtagcggt agaagcaggt gccaaacgcc tcctactcaa
    35521 ccatatcagt gcccgtttcc tctcaaaaga tattagcaaa ctcaagaaag acgctgccac
    35581 aatttttgaa aatgtccatg tggtcaaaga cttggaagaa gtggaaatct agcagtcaca
    35641 gaaaggataa gtatgcctac tattctcatt accggagcta gcggtggtct agctcaagaa
    35701 atggtcaaac tcctgcccaa tgaccaactc atcttgcttg gtagaaataa ggaaaaatta
    35761 gcccaactct acggaaatta ttcccatgca gaattgattg aaattgatat taccgacgat
    35821 tcagccctag aagctctggt aactgatctt tatctccgct atggcaagat tgatgtcttg
    35881 attaacaacg ctggttacgg gatttttgag ggatttgacc agattgctga taaagatatt
    35941 caccagatgt ttgaggtcaa tacctttgcc ctgatgaatc tgtctcgtca ccttgcggct
    36001 cgtatgaagg aaagcagcaa agggcatatc atcaacatcg tcagcatggc aggtctaata
    36061 gctactggca agtctagtct ttactcagcg accaagtttg cggctattgg tttttcaaat
    36121 gctctgcgac tcgaacttat gccctatgga gtctatgtga caacagtcaa tccaggtcca
    36181 atccgaacag gatttttcga ccaagccgac ccagatggaa cttatcttaa atcggttgac
    36241 cgcttcctgc tagaaccaga tgcagtggct aaaaagattg tcaagattat aggaaaaaac
    36301 aaacgagaac tcaatctccc gattttgttg aacctagctc ataagtttta taccctcttt
    36361 cctaagctag ctgataagtt ggcaggggaa acttttaatt ataagtaaaa agaaccaatg
    36421 tgcaggttgt tgctagccta catattggtt cttatcattt ctcaactatc aaactgaact
    36481 tcttctagga gatagtctat aaagcgttcg cccatcttag ataggctggt tttctcatgc
    36541 tggatataga ccagctcgat cgggtcatca atatccagtg ggatagaaac gatattgtct
    36601 ccgtttaggt tgctgttcaa aatccctgtc gcaatggtat aaccatccaa accaatcaag
    36661 agattaaaga gggtggcacg gtcactgacc acaatggatt tcttgtggtg ttcttgagaa
    36721 agaatctctt ctgaaaagta gaaggagttg tgcgtccctt ggtcatagct gaggtaaggg
    36781 aaattctcca aatcagacag tttcaccttg tctttctttg ccagagggtt ggtcttgctg
    36841 acaaagatat gtggttgcgc tgtgaagaga tggtgggcta gcaggtgatt gtcatccagc
    36901 atcttggtta aaacatcacg gttgtaactg tttaagaaga ggaccccgac ctcactgcgg
    36961 aagttcttga cgtcgtcgat aatctcccaa gtccgagttt cacgaaggaa gagttcgtat
    37021 ttctccatat cgcttttctt gagcaaagag acaaaggcat tgaccacaaa ggcatagtgt
    37081 tgagacgaaa cgctaaatag ttcgcggtgg gcgacaggat ttttatagcg ttcctccaga
    37141 agctgggtct gctcgacaac ctgacgggca taagagagaa actccatccc atcacgggtc
    37201 aaggtgattc ccttgggatt gcggataaag atctcaatgc ccatttcatt ttccaaatct
    37261 cgcactgcat tggagagact tggctgagtg ataaagagtt gcttggctgc ctcattcatg
    37321 gagccagttt cgacgatttt gataatataa tgtaattgtt gaattctcat gtttttattg
    37381 taccatactt gccttagaat cgctaaagaa gataggaaaa taaggagcat tgtgtgagtc
    37441 tcttcttgaa aaaaacacaa aattttgcta gaatgagtct tatgaaaaca ttctatgatg
    37501 tgcagcaatt cctcaaacga tttggtatta ttgtttacat gggaaaacgc ttatatgata
    37561 ttgaactgat gaagttggaa ctatctcgga tttacgatgc agggttgatg gataaattag
    37621 actatctaga ggcagaagca gttcttcgca gagagcacaa ggtagaattg gattatattg
    37681 agaaaaatgg agaaaagaac taatggttac ttggattttg tgggcactta tactagcaat
    37741 gttggcgtgg atgggcttta actatcttcg tattcgccgt gcggctaaaa ttgtggacaa
    37801 tgaggagttt gaagccttga ttcgtacggg tcaattgatt gatttgcgcg acccagcaga
    37861 attccacaga aaacatatcc ttggtgcacg caatattcct tcaagtcagt tgaaaactag
    37921 tcttgcagcc cttcgtaaag ataaacctgt ccttctctac gagaaccaac gtgcgcaacg
    37981 agttacaaat gcagctcttt acttgaaaaa acaaggtttt tctgagattt atatcctttc
    38041 ttatggcttg gattcttgga aagggaaagt gaagactagc taaccttatt tccaaattag
    38101 tcgaatgata ggatgaagct attgttttat agtataatta atttgtataa aatttaagga
    38161 gttcttagaa cataaaagaa aagaagaaaa atccactttt tgtgggtatc ttgtcgatta
    38221 ttcttggttt gttatttcca attgtaggcc tgattttggg tatcatagga ttggtcttgg
    38281 ctatttcata ccaaaaagag tcataattag actataaaat agaaaagatt cttaatatct
    38341 taggaattgt gatttctgta gttaactgga ttgtagcaat cgccttaatt tttcggtaaa
    38401 taaaagaaaa aagctttagt tttaaagctt tttttgtttt atcgactcat ctctaaatag
    38461 tttttaatga tttgtaactc tttttggttc aaagggcggt agttactttc tgctaaatct
    38521 gggttcaatg tgaagtcccc aaattggatt ctcttgagga tagtcacctt aacaccaacc
    38581 gagaggaaca ttttcttgat ttgatgaaat tttccttctg aaatggtgat agaggcttga
    38641 ctgatggagg gacttgcaga tagaatctct agttttgcgg gtttacagac agtaccatct
    38701 aaaaagacaa ttcccttttg aaaggcttgg atatggtcag gtgttagaag tccattaacc
    38761 tcaacttgat aagtcttatc gacatgatat tggggatgga ggagttggaa gcccaaggga
    38821 ccgttatcgg tcaagaggag gagtcccgtt gtatctcggt ccagtcggcc aacggcatag
    38881 agcttgtcag actggatgtc agatggaagc aggtccatga cggtcggaag ttccttgtct
    38941 ttgttggctg taacgacacc agcaggttta tgaagcataa gataggtgtg ttcatagcct
    39001 tgaatgattc gatcctgaaa aaggagttct tgtagtcctg tatcgatatt ttgagctagg
    39061 gagcgggctg ggcaaccatc gactagaatt ttccctctga ggagtgcttg cttcatggcc
    39121 tttcggctga ttttttcttg ggctaataaa ttatctaaac gcatactgtg cctatcttat
    39181 cataagtcgc ttgctttgaa aaggcttgac gtgaaaagaa aagcggagtg aaaacattta
    39241 cacttgcttg agttatgtta tttatttgaa attatggtat aatcgttcag ttagaaaata
    39301 aattttgaat attatagagg aaatcatgac aaaattaaga gaagatatcc gtaacattgc
    39361 gattatcgcc cacgttgacc acggtaaaac aaccctggtt gacgaattat tgaaacaatc
    39421 agaaacgctt gatgcacgta ctgaattggc agagcgtgct atggactcaa acgatatcga
    39481 aaaagagcgt ggaatcacca tccttgctaa aaatactgcc gttgcttaca acggaactcg
    39541 tatcaacatt atggacacac caggacacgc ggacttcggt ggagaagttg agcgtatcat
    39601 gaaaatggtt gacggtgttg tcttggtcgt agatgcctat gaaggaacca tgccacaaac
    39661 tcgtttcgta ttgaaaaaag ccttggaaca agaccttgtc ccaatcgtgg ttgttaacaa
    39721 aatcgataag ccatcagctc gtccagcaga agtagtggat gaagtcttgg aacttttcat
    39781 cgagcttggt gcagatgacg accagcttga tttcccagtg gtttatgctt cagcgatcaa
    39841 cggaacttct tcattgtcag atgatccagc tgaccaagaa gcgactatgg caccaatctt
    39901 tgacacgatt atcgaccata tcccagctcc agtagataac tcagatgagc ctttgcagtt
    39961 ccaagtgtca cttttggact acaatgactt cgttggacgt atcggtatcg gtcgtgtctt
    40021 ccgtggtaca gttaaggttg gggaccaagt taccctttct aaacttgacg gtacaactaa
    40081 aaacttccgt gttacaaaac tcttcggttt ctttggtttg gaacgtcgtg aaatccaaga
    40141 agccaaagcg ggtgacttga ttgccgtttc aggtatggaa gacatctttg tcggtgaaac
    40201 catcactccg acagatgcag tagaagctct tccaatccta cacatcgatg agccaactct
    40261 tcaaatgact ttcttggtca acaactcacc atttgctggt aaagaaggta aatgggtaac
    40321 ttctcgtaag gtggaagaac gcttgcaggc ggaattgcaa acagacgttt cccttcgtgt
    40381 tgacccaact gattcaccag ataaatggac tgtttcagga cgtggagaat tgcacttgtc
    40441 aatccttatc gaaacaatgc gtcgtgaggg ctatgaactt caagtatctc gtccagaagt
    40501 tatcgtaaaa gaaatcgacg gtgttaaatg tgaaccattt gaacgtgtac aaatcgacac
    40561 tccagaagaa taccaagggt ctgttatcca aagcctttct gaacgtaaag gtgaaatgtt
    40621 ggatatgatt tcaactggta atggtcaaac tcgtttggtc ttccttgttc cagcgcgtgg
    40681 tttgattgga tactcaactg agttcttgtc aatgactcgt ggttacggta tcatgaacca
    40741 taccttcgac caatacttgc cattgattcc aggggaaatt ggtggacgtc accgtggtgc
    40801 ccttgtttct atcgatgctg gtaaggctac aacttactca atcatgtcta tcgaagaacg
    40861 tggtacgatc tttgtcaacc caggtactga ggtttatgaa ggaatgatca tcggtgaaaa
    40921 ctctcgtgaa aatgacttga cagttaacat cacgaaggca aaacaaatga ccaacgttcg
    40981 ttcagctact aaggaccaaa cagctgttat caagacgcct cgtatcttga cacttgaaga
    41041 gtctcttgag ttcttgaacg acgatgagta catggaagta acgcctgagt ctatccgttt
    41101 gcgtaaacaa atccttaaca aagcagagcg tgagaaagct aacaagaaga aaaaatcagc
    41161 tgaataagag ctagaaagag agaaagatgg tctatttagt cctaggaatt ttactgctcc
    41221 tactctatgt atttgcgaca ccagaaagca ttaaagggac agtcaatatc gtcgctatgg
    41281 tatgtatttt agtggcactc ttgattttat tggttctatc ttttctgaaa atttttcaat
    41341 taccaacaga aatattccta gcaatagcca tgttgctcct agcttacttt agtgttagag
    41401 acattacact catgccagtc aaaaaaagta aaagaagata aaaaagagag ttttggctct
    41461 cttttttgct aggaatttta caagattgca atttattttt gagttatcac catctctttt
    41521 gtttggataa tatgaatggg aaaaacttga ataaaattcg ttaatggaat ctattttata
    41581 tttggttagt actggttgca tattattttg aaataaaact actatataat attattcata
    41641 gaggtaatat agtaaaatat ctaaaaaatt cgattttttc cttgacaaca aataataaaa
    41701 atgttattct tatcacataa gaaagcggtt tcaataaaag agagaggagg atttattttg
    41761 ttgcattgtt gctcaaggtg tgattttttt gaaagaaaaa ataaaatgaa aacgaaaaaa
    41821 catagattac ttgctctagc tcttatttca agttttacat tattgggagc tgcatcagct
    41881 gctgtataat atccagatgg aggagtatgg acatatggag aaggttcagg aggtggttag
    41941 gctttttcaa attactatca tggtaaaaaa tatcattatt catccatcgt gagcagatgg
    42001 gatggtcatt ctgataaagg agaagctcct gctggaaaaa cctcctatgc atggatttgg
    42061 actaaatggg gagaacaagt agcattttac tgtgattatg actaatcgtt cattgattta
    42121 aatcgttccc cttgtaagaa attacagggg gaattttgtt tacaaggagt ctaccaagga
    42181 gtctactatg aagaaactat tcatattatt atcaactttt tttctcagct tcttccttgc
    42241 ttggattatt gtcttacgtg cgccacaata tttatatgca agctatgatt ccgtttcttt
    42301 acttcgtgtc aaaaaagata ctcaggaacc gacgcgtgag gtatttgaac aggaattgga
    42361 gaattttgca aactcagaac agagtttaat agctagaaga attgtagagc cgagtaagga
    42421 tgggacgact cactttactt atgcaactta tggtcaggga actttaccaa aagaattcca
    42481 agaagctagc caagaaagtc gtgaacgtag tgatccgcta aatagttatc tccttttgtc
    42541 aggctccttg acgaaagaaa agcttgccga taaattagga gatttgggtt ataaagcaag
    42601 tgctgaccga aagataccgc cctattttct tgcttttcga atattactaa atccccttat
    42661 tttaattagt ttagcaatat ttggcttatc tttctttgct ttagtgatta tcactcggat
    42721 taaggaaatg agagcagcag gtataaaact cttttctggt cagactctct tatccatcat
    42781 ggggcattct ttatctactg atatcaaata gctccttcta tcagccctcc tttccttcct
    42841 aggtggggga gtcgttcttt ttagtcaagg tttgttttat cctatcttgt tagccaccta
    42901 tggttttggg attagtttct atctgttgtt tttattggcg atttcaattt tactaatgct
    42961 tctttatcta atgagtttga gttacaaagc attagttccc gttattaagg ggagattacc
    43021 ccttaaacgc ctgatgattt taaccctatt gtgtcagttg gttgctgtct ttacagtagg
    43081 atacgctgtt aagacaggtt tgacgtctta ccaacgattg aaagaacttg aaatttcaaa
    43141 acaagcatgg caggatagag cagactatta tcaaatttct tttggcttag gtgatagagg
    43201 aaaagataca gaaaatcaga gcaagtggta tgcctttgcc aaggaagcaa tcgaagagga
    43261 acaagctctt tatgtaaagg ataatctgct ccattttgcc aatccacaag gaaaaaatga
    43321 acagggagag acactggata cctatagtcc agatgctaat acgctctatg ttagtcccag
    43381 ttatttggac aaggaaaagg tcgtggtaga tgctgagacc aaacagaagt tagcccatct
    43441 ccaaaaaggt gagtttatcc tcttgctccc agaacatttg cgctctcgag aagcagaact
    43501 taagaaagtt tttgaagaaa gattgagtta ttatggaaaa tctggtgagg aggcaagtgc
    43561 tcctttggat tatgagatga aagcgcacgt tagttatctt tcaatgggag aaaagcggtt
    43621 tgtttataat aacggtgaga atcccgtatc tactcagtat ttgactgatc cgattttagt
    43681 ggtattcacg ccgacttcta caggtgatag ttttatatcc ttatctagtt ggtctatcaa
    43741 tgctggaaaa caactcttta tcaaaggata tgagagtggg ctagaactct tgaagaaagc
    43801 tggaatttat gagcaagtat cctatcttaa agaaggaaga agtgtttatc taactcgtta
    43861 taatgaagtt caaactgaaa cagcaacttt aatcttagga gctattgtgg ggatagctag
    43921 ttccttatta ctattttatt ctgtcaatct tctatatttc gagcaattcc gccgagatat
    43981 cttgattaaa cgaatttcag gtttacgatt ttttgaaaca catgctcagt atatggttag
    44041 ccaatttgcc agttttgtat ttggtgctag tctctttatt ttaagcagtc gagacttggt
    44101 gattggcttg ctcactttat tggtctttct agctagtgca gttttgacgc tttaccgtca
    44161 agcgcagaaa gaatctcgtg tttctatgac aattatgaaa ggaaaatagg atgattgaac
    44221 taaagaatat atctaaaaaa tttggaagcc gtcagctatt ttcagatacg aatcttcatt
    44281 ttgaaggtgg gaaaatttat gccttaatcg gtacaagtgg ctgtggtaag acaacactct
    44341 tgaatatgat tggacgattg gagccatatg acaaagggca aatcatctat gatggcactt
    44401 ctcttaagga catcaagcct tctgttttct ttagagatta cttaggatac ttatttcaag
    44461 attttggctt aattgaaagc caaaccgtca aagagaatct caatctgggt ttagttggta
    44521 aaaagttgaa ggaaaaagag aaaatctctt tgatgaaaca agctctaaac tgtgtaaacc
    44581 tctcttattt ggatttgaag caacctatat ttgagttatc aggaggagaa gcacaacgtg
    44641 ttgcactagc gaagataatt ttaaaggatc cgcctttgat tcttgcagat gaaccaaccg
    44701 cttccttaga ccccaaaaat tctgaggaat tactttccat cctagaatct ttaaaaaatc
    44761 cgaatcggac cattattatt gcgacccaca atcctctgat ttgggagcaa gtggatcagg
    44821 tcattcgagt taccgattta tcacatagat gatatggtaa gattcagtta gaagaaagag
    44881 tcacaaacac actttgtggc ttttttattt ccataaaaat ggtaaaatag taggagtaga
    44941 aatggagttc gagacatgaa agtaatagat caatttaaaa ataagaaagt tcttgtttta
    45001 ggtttggcca agtctggtga atctgcggct cgtttgttgg acaagttagg tgccattgtg
    45061 acagtaaatg atgggaaacc attcgaggac aatccagctg cccaatgttt gctggaagaa
    45121 gggatcaagg tcattacagg tggccatcct ttggaactct tggatgaaga gtttgccctc
    45181 atggtaaaaa atccaggtat cccctacagc aatcccatga ttgaaaaggc tttggccaag
    45241 ggaattccag tcttgactga ggtggaattg gcttatttga tttcagaagc accgattatt
    45301 ggtatcacag gatcgaacgg taagacaacc acaacgacta tgattgggga agttttgact
    45361 gctgctgggc aacatggtct tttatcaggg aatatcggct atcctgccag tcaggttgct
    45421 caaatagcat cagataagga cacgcttgtt atggaacttt cttctttcca actcatgggt
    45481 gttcaagaat tccatccaga gattgcggtt attaccaacc tcatgccaac tcatatcgat
    45541 tatcatggat tatttgagga atatgtagca gccaagtgga atatccagaa caagatgaca
    45601 gcagctgatt tccttgtctt gaactttaat caagacttgg taaaagactt ggcttccaag
    45661 acagaagcca ctgttgtacc attttcaaca cttgaaaagg ttgatggagc ttatctggaa
    45721 gatggtcaac tctacttccg tggtgaagta gtcatggcag cgaatgaaat cggtgttcca
    45781 ggtagccaca atgtggaaaa tgcccttgcg actattgctg tagccaagct tcgtgatgtg
    45841 gacaatcaaa ccatcaagga aactctttca gccttcggtg gtgtcaaaca ccgtctccag
    45901 tttgtggatg acatcaaggg tgttaaattc tataacgaca gtaaatcaac taatatcttg
    45961 gctactcaaa aagccttatc aggatttgac aacagcaagg tcgtcttgat tgcaggtggt
    46021 ttggaccgtg gcaatgagtt tgacgaattg gtgccagaca ttactggact caagaagatg
    46081 gtcatcctgg gtcaatctgc agaacgtgtc aaacgggcag cagacaaggc tggtgtcgct
    46141 tatgtggagg cgacagatat tgcagatgcg acccgcaagg cctatgagct tgcgactcaa
    46201 ggagatgtgg ttcttcttag tcctgccaat gccagctggg atatgtatgc taactttgaa
    46261 gtacgtggcg acctctttat cgacacagta gcggagttaa aagaataaaa tatgaaaaaa
    46321 attgtcttta caggtggggg gacggttgga cacgttaccc tcaatctttt gttaatgccc
    46381 aagttcatcg aagatggttg ggaagtccac tatatcgggg acaagcgtgg tatcgaacac
    46441 caagaaatcc ttaagtcagg tttggatgtc actttccact ccattgcgac tgggaaattg
    46501 cgtcgctatt tctcttggca aaatatgctg gacgtcttca aagttggctg gggaatcgtc
    46561 caatcgctct ttatcatgtt gcgacttcgt ccacagaccc ttttttcaaa ggggggcttt
    46621 gtctcagtac cgcctgttat cgcagcgcgt gtgtcaggag tgcctgtctt tattcacgaa
    46681 tctgacctgt ctatgggctt ggccaataaa atcgcctata aatttgcgac taagatgtat
    46741 tcaacctttg agcaagcttc gagtttgtct aaggttgagc atgtgggagc agtgaccaag
    46801 gtttcagatc aaaaaaatcc agaaccagat gaattggtgg atattcaaac ccactttaat
    46861 cataaattgc cgactgtatt gtttgttggc ggttctgcag gtgctcgtgt ctttaaccaa
    46921 ttggtgacag accataagaa agaactaaca gagcgctaca atattatcaa tctaactgga
    46981 gattctagcc tgaatgagtt gagccaaaat ctttttcgtg ttgactatgt gaccgatctc
    47041 tatcaaccct tgttggaatt ggctgatatt gttgtgacac gaggtggtgc caatacgatt
    47101 tttgagctct tggcgatagc aaaattgcat gtcattgtgc cgcttggtcg tgaagctagt
    47161 cgtggtgacc agattgaaaa tgcagcttac tttgtaaaaa aaggctatgc agaagacctt
    47221 caagaaagcg atttgacctt ggatagtttg gaagagaagc tttctcactt actaagtcac
    47281 aaggaagatt accaagctaa gatgaaagct tctaaggaat tgaaatctct agcagatttt
    47341 tatcaattgt tgaaaaaaga tttatcataa ggaaagtaaa tgtcaaaaga taagaaaaat
    47401 gaggacaaag aaatcctcga agaattgaaa gagttatcag aatggcagaa acgaaaccaa
    47461 gaatatctaa aaaagaaggc tgaagaagaa gcggctctag ctgaggagaa ggaaaaggaa
    47521 agacaagctc ggatgggaga agaatctgag aagtcagagg acaaacagga ccaggagagt
    47581 gaaacagacc aggaagattc agaatcagct aaggaagagt ctgaagaaaa agtagcatcc
    47641 tcagaggatg acaaagagaa agaagagaaa gaagaaccag agtctaaaga gaaggaggaa
    47701 caggataaaa agcttgctaa aaaggctaca aaggaaaaac cagccaaagc aaagattcct
    47761 ggtatccata tcttgcgagc cttcacgatt ttatttccaa gtctgctttt attgattgtc
    47821 tctgcctact tgctcagtcc ttatgcgacc atgaaagata ttcgtgttga gggaacggtg
    47881 caaactacag ctgatgatat tcgacaggct tcaggcattc aggattcgga ttatacgatt
    47941 aaccttctgc tagacaaggc aaaatatgaa aagcagatta agtctaacta ttgggttgaa
    48001 tcagctcaac ttgtctatca atttccaact aagttcacta ttaaggtcaa ggaatatgat
    48061 attgtggcct actatatttc tggtgaaaat cattatccta ttctttccag tggtcagctt
    48121 gagactagtt ctgtgagcct gaacagttta ccagaaactt atttatcagt tctctttaat
    48181 gatagtgaac aaatcaaggt ttttgtctca gaacttgctc aaattagccc agaactcaag
    48241 gcggctatcc aaaaggtgga attagcccca agcaaggtga catccgattt aattcgattg
    48301 actatgaatg atttggacga agtcttggtt ccgctttctg aaatgagcaa gaaactgcct
    48361 tattatagta agattaagcc acaattgtca gaaccgagtg tgattgacat ggaagctgga
    48421 atttacagtt acactgtggc ggataaatta attatggaag ctgaggaaaa agccaaacaa
    48481 gaggctaagg aagctgagaa aaaacaagaa gaagaacaga aaaaacaaga ggaagagagc
    48541 aatcgaaacc aaacgaccca gcgctcatcg cgtcgctagg tttacctttt ctcttatagt
    48601 tctttagtga ccatgttttt acttgacaag tgctagtaga aataatagaa tagtaaaaaa
    48661 cctttaaagc agtccagaga ggcagctaag gttagacggt gaaagggtgg agactaccca
    48721 tttttcgtgg aaccttgctg ttggcaggtt ccttttttcg tggcttctgt tggccagact
    48781 ctctcactag taaaggtaaa aggagaaacc tatgcgagaa catcgtccag tcattgctct
    48841 tgattttcct agttttgagg cggtcaagga atttttagct cttttcccag cagaagaaag
    48901 cctttatctc aaggtaggga tggagcttta ttacgcagcg gggcctgaga ttgtgtccta
    48961 cttaaaaggt ttgggtcata gtgtcttttt ggatctcaaa cttcatgaca ttcctaatac
    49021 agtcaagtca gccatgaagg tcttgtctca gcttggtgtc gatatgacta atgtccatgc
    49081 ggctggtggt gtagagatga tgaaggcggc gcgtgaaggc cttgggagtc aagctaaatt
    49141 gatcgctgta actcagctca catcaacgtc agaagctcag atgcaggagt ttcaaaatat
    49201 ccaaaccagt ctgcaagagt ctgtgattca ctatgccaag aagacagctg aagctggctt
    49261 ggatggtgtt gtttgctcgg ctcaggaagt acaagtcatc aagcaggcta ccaatccaga
    49321 ttttatctgt ctgacaccag ggattcgtcc agctggtgct gcagttggag atcaaaaacg
    49381 agtcatgaca cctgctgatg cctatcaaat cggcagtgac tatatcgtag tgggacgtcc
    49441 cattacccaa gctgaggatc ctgttgcagc ttatcatacc atcaaggatg aatggacaca
    49501 ggactggaat taaagaacta gattagaaaa ataaaaggag aataccatga cacttgctaa
    49561 agatatcgct agccacctct tgaaaatcca agccgtttac ctcaaaccag aggaaccctt
    49621 cacttgggca tctggtatca agtcaccgat ttacactgat aatcgtgtga cactagccta
    49681 tccagaaact cgtaccctaa ttgaaaatgg ttttgtggaa gctatcaaag aagcctttcc
    49741 tgaagtagaa gtgattgcag gaactgcaac agcagggatt ccacacggag ccattattgc
    49801 tgataagatg gacttgcctt ttgcctacat ccgtagcaaa ccaaaagacc acggagctgg
    49861 taatcaaatc gaaggtcgcg tagctcaagg tcaaaaaatg gtagtggttg aagaccttat
    49921 ttcaacgggt ggttcagttc ttgaagctgt agcagcagcc aagcgagaag gagcagatgt
    49981 gcttggagtt gtagcgattt tcagctacca attgccaaaa gcagataaga actttgcaga
    50041 tgctggtgtt aaacttgtga cgctttcaaa ctatagcgag cttatccatc tagcccaaga
    50101 agaaggttac atcacgccag agggccttga tcttctaaaa cgctttaaag aagaccaaga
    50161 aaattggcaa gaaggttagg tcagtaagat aaagagagat gaggctaccg agcctctttt
    50221 atcattttat ttaaaatatg acaatatttt aaataaaatg attgacaacg ctttcttttg
    50281 ttgatataat gaaaatgtaa tatgtatcaa attgatcaat caactatctt gtttatcact
    50341 tcttaggagg acgatgtcat gaaaagaaga aaaatatatt ccttttttgc gtagttacaa
    50401 ttgcatctag tttgtttctc cttacagttt tagctagaca gattggagat tatgatttaa
    50461 cgttgccgcg ttggggttcg gatacaacta gtgagcttga gaaagaaaac gcctctgctg
    50521 gaattaataa taatgacagc actggtggcg gtaaaaggtt aaatacctct attcgtagcg
    50581 cctatagtgg gtcagatatt accccggtat attcattggg gtctggctct aggattgtca
    50641 tgtactataa tggaggtggt gacaattata ttggttctgg tactagatta gctatggcgc
    50701 cacaatttgg aaatcatgta agaattcata cttcaggttc ttggaatcca gattcttatt
    50761 aacttacttg tcagagtaag ccttagagat ggttgattgt gggtgtagca tgaaaaaaga
    50821 atgctacacc ctatttttat tataaggagg agtaaggatg gaatttttca tttgtaatct
    50881 tgtacgagtc gttcaatcac ctcgatttta tatgtcttta tttttgaccc ttctttgcat
    50941 gagtttagga aatttccttg ctttcaatgg tatttataaa attgaaggtt tatcgatttt
    51001 ttttgccgct tcttctattc gaggattttc accgattagc ctagtagctg cacttatctg
    51061 tacactgccc tattctagtc agataataga ggatgctgag agtcattttc taacagcaca
    51121 attgtgtcga atttctaaaa agaagtatct ggctattgtg ggtagtactg taattatttc
    51181 ttcttttcta gtcttttttc tcccctattt attattatta ggaattaatc ttttagtgac
    51241 tccttatcag gaaatttata ttggagatta tagtggtgcc ttaaaagaat tatttgattc
    51301 caatcagttt ctctatagtc ttgtaacgac tctctggtat ggagtttggg gcgctgtgtt
    51361 ctctattttt ggactagcta gtgctttgct agtgaagaaa aaaataggag ctattttcat
    51421 cccagttgcc tatatgatgg ttggtggtat tttttgggct attttagggc tatcttactt
    51481 agaacctgtg acaacattag ctttgggata tcagaaagat atcagtcttt ccttagttag
    51541 tgggcatctt gcttttattt tatttgttag ttgtttggtt gtttatggta cattttttct
    51601 acattcagag gactacgtat aatgaaacaa tttgttcaat tttacaaaaa agatttttta
    51661 tcagtattgg tttattttat attactgcta tcctgtgttt tatctagtac agtatattta
    51721 ttgcgctgtc gccaatattc aatccatcca aatgtattag aatggatctt agttttactt
    51781 caagatatga cgactggagt atattgcttt ccgttcacat atatattgtt ctttttttat
    51841 ttgatgaata actattttaa taggttggag tgtcgcattc gtctgaaatc aattaagcac
    51901 tttaccagtt ttagtttcaa attagcagct cttagtacgg ggatttggac ggcgacttta
    51961 tttttattga tttttctaat tgcatttagt aatggtttta gcttctcttt ggagataaag
    52021 gaggttgatt ttttaagaga attttatggt ataagtattg caaacaatgc tagtttcttt
    52081 ataggatttt ttttctctta tatagcatac tatttctttt tatccttact tactattagc
    52141 agtttttctt ggtttaaaaa atcaaacatg agcttagtat ttctgtttac ttttttattt
    52201 gtagaatcct tattctggat ttatcagttg gacaatggga taattggatt attgccaatt
    52261 tttcagtata tggtaaattc caatccgtat gcattgattt attggcttac attactatct
    52321 atcataattc cattgactgt attttctgtt catagaaact ggaggagagt gtaaaagttg
    52381 gaaatgggaa agttaagtag tcacatgtgg aggttgaatc agataatcta taccaagtac
    52441 ttttggggtt atgttctttt ttggatattg atttgtttag gattatggta ttggttagaa
    52501 ggaaatgata gacttgttat agaaatttta aaagggccta atctgagtca aaactctttt
    52561 ttagtcttat ctatatggtt gcttcattgg tttattattc atacattttt tctagcagtt
    52621 gtatatcgta gaagagcatc cgatttcttt atggaagtga ttcgattttc ttctattaag
    52681 ctctggatta ggtatcagat ttggacctgt tttctttatg gactcatttt aatcatggta
    52741 aaagttctag tgattcaatt tatgttacag ttaccaaact gggatatagg agttttgttt
    52801 atagttgatt ctttgaatgc ttgtgtgtta gtcttgtttt gctttatgtt atacgcacta
    52861 ggagcgaatg tacaaatgaa ctttgcttgc gttagtttct ttttactcat gattgtgttt
    52921 ggtggtctat ttgtaggaaa tcgaactaat tatctatttt atattttgaa tcgagggaat
    52981 ggagatatag gacgagattt atttcttcag ttgctatttt tagtctttct ttttaaaagt
    53041 attttctatt tcactcgtca aaagaggagg tttatagaat gaatgaaata atcatattaa
    53101 aaaatattga gttgaaatta aaaaaaacat gtgtttttca aaaccttaat tttagttgta
    53161 aacaggggga aattatagga attactggtg cgaatggctc agggaaaagt gtattgttta
    53221 aattaatagc tggtttatat agtccgtctt atggagaagt gttaatcaat ggggaaaata
    53281 ttgttcctga gagaaaaatt ccagctaatt tgggagcttt gattgaagaa cctggtttta
    53341 taaattatta tagtggcttt aagaatttac aatatttggc aagcatacga ggagtagttg
    53401 gtaatcagga aatcaatgat acactgaaaa tagttggtct atatgagcaa aaagaccaga
    53461 aagttaaaac ttattcgcta ggtatgagga aaaagctagg gattgctcaa gcaattatgg
    53521 agaatccctc tattctttta ctagatgaac ctatgaatgc cttggataaa tcaagtgtag
    53581 aaaatatgag aacattgttt agaaagctct ctagtgaaaa agggacaaca attttgattg
    53641 ctagtcatag tgaagaggat attcgtatct tatgtgataa agtatatgca atagaagata
    53701 aagtatgtac actgtgttca gattgaacat cttagataga ctcaggatgt tataggtaaa
    53761 atcttaaaaa gggattggag cttaaaatcc agtccctttt aagagttaat ggccaccttc
    53821 aacaaccagg tcatctgctt tagcagcgtc accgtaggtt gggtgaagtg ttttttcata
    53881 ggccttgtgg aagaagtttt ccttgcctaa tttttcaata tctttattga tgaagtctag
    53941 caattcttgg ttgccttttt gaactgctgc cgcaatggta tcgggatcac cgagggaagt
    54001 aattcctact tcaaatcctt tattttcaag cgcccaagct agaacttccg tattgtcagt
    54061 tgaaaaggca tctccacgtc cgtcaagaag agcttggtaa gagtcactgt attggtcgta
    54121 tttttggagt ttgatttctg gatgattctt ttcaaaataa gtctcagcag tcgttccttt
    54181 tgtgacaatt aaggttttac cttcaagttg tttgacgtct gtaatgagac cagtcttagg
    54241 tgatacgaca cccagagaaa ctttcatata tggaagggca aaatcaactt gtttcttacg
    54301 ttcgtcagtt actgtaaagt tagcaagagt aatatctacc ttgtttgaaa tcaagtattc
    54361 cgcacggttg gcagcatcga ctgaaatgta tttaaccttg acaccaaggt ctttagctag
    54421 ttggttccct agttcaatat cgtagccttg gtaagaacca tcattgtcaa cgtagccaaa
    54481 cggtttttta tctccaaaca cggcgattcg cagttcaccg cctttttttg atttcatcga
    54541 tagtgcgagc tttggcagtt gtttttccag aggatgaacc agcgtttcca cctgagctac
    54601 aagcagtgat aaagataagg gcaaaggcaa gtgctaaaac agttaagagt ggtttgaata
    54661 gtttcataag aatctccttt atagatatga gccaaattgg ctaaagtcaa agacgtttaa
    54721 aaattcctgg gctcgtttgg tttgcggatt ggtaaagaag gcttgagctg ttccttcttc
    54781 agcgattttc ccttggtcga ggaagataat ccggtcagca atggcttggg caaactgcat
    54841 ttcgtgggtt actaaaatca tggtacggcc ttcttgggcc aaatcattga taagttccag
    54901 cacctcacgc accatttctg gatccagcga agcagtcacc tcgtcaaaaa ggatgatttc
    54961 tggatgcatt aggagggcac ggacaattgc aacacgttgc ttctgtccac cagataattg
    55021 acgggcaaag ctatgttgtt tatccagcaa accgacacga tctagtaatt gcaaagcttc
    55081 ttccgttact tctttcttgt cccttccttg agttttgata gggcctagga tgaggttttg
    55141 taagacatcc agatggggaa agagttcata actttgaaag accatgccaa tcttttggcg
    55201 aactaggtga aaattttttt tattttcaac gatagactga ccatccagaa aaatatctcc
    55261 accttgaata ctttctaagc cgttgaggca acgaaggagg gtacttttcc cacaaccaga
    55321 tggccctagg atgacaacaa cttccccttt tttgatttct agagaaagtc cttggaggat
    55381 gggattgtct ccgaaggatt tttttagttc cttgatttct aagatagttt cagacattta
    55441 gtttctccaa tgtttttcta agtgagtgga tagtttggaa atagggtagc aaactgcgaa
    55501 atataagact agaatggttc cataaatcca aaatgaagca gttgggatgg tcaggcgatt
    55561 gctatcgatg atttgttgtc caactttggt cacttccaca accccaatca aaacaactaa
    55621 tgaagtggtt ttaatcatcc gagtgacaag attgatagcc tgcggtagca gtcttctcaa
    55681 gacttgtggg atgatgatgt ggtagtaaag ttgaacatta gtcaagccga gtgcctgtcc
    55741 actttcaaac tgatgtttag ggagagaagt gatagctcca cgtaccaagt ctcccatttc
    55801 agctgttccc cagagggtaa aaacgataat agctgaagtc tcacctgaga tattgatatt
    55861 aaagtttcga gccaagccaa agtaaacgat gaagagtagc accagctggg gcatgatacg
    55921 gataaattcc agatacaatc gtgttaaaaa tcgtatgatt ctagaatggg aggtcatgat
    55981 gattcccatg actgttccga acatcatgga taagaggaca gacaggatgg atatcccaat
    56041 cgtaacgccc aatccctgta agattctcag gagattattt ccttgaaaga gtacttggat
    56101 tcccgaatcc tgcatggcgg agcctccttt ctatccagct aaagaccagt gagatgggta
    56161 gcagcatgat cagataagca actaccaaca tagctagcgc aatgtctgtc tcatagtaga
    56221 gaccaatcaa atccttggcg acgtacatga ggtcggccaa agccactgct gagaaaacag
    56281 aggtttcctt gataaggaaa atgacattgg cactaaagga ggggagtgcc accgctgttg
    56341 cttgcggaag aaccacatag taaaagacct gtagaggtgt cagaccaata gcgaggccaa
    56401 tctcctgctg ggtttgactg atggcttcca gcccgcttcg gaaagattct gccatatagg
    56461 agtctcctaa aaagacaagc ccaagcgttg cacagacttc tgaagatagg acaatcccga
    56521 ttcggggaag accgaagtag agaaagaaga gttgaatcaa aaggggcgta ttacgtgaca
    56581 attcaatgta ggctgtcgct acttgcgaca aaacaaggat tcgataatgt cggatgatac
    56641 taacgattaa accgagcaga aaggatccca aaattcccca aactgcaata tgcaaggtca
    56701 gaaagaatgc cttttgatat agtggtagat attgttcaac aatggatcaa tccaaaaata
    56761 gaacctccca tctagaaata atacagttat tgtagcactt aaaatcttct ttggataata
    56821 tctatttttt attgccgtta taaggatttt tatcatagac ataaaatttc tgaaatttcc
    56881 aaacaaaata ttttaaaagt tttgaaaaag agttaagata tttttgtaat acacaaagta
    56941 aacgcttact tattaaggag gacattttat gtcatacaaa acaagcaatg cagaaggtca
    57001 tgtagatttc atcaatacct atgatttgga gccaatggcg caacaagtta ttcctaaagc
    57061 agcatttggc tatatcgcta gtggggcggg agatactttc acttctttcc agtgatttta
    57121 gcgtcaggtt ctttttagtt tttaaagatt atccgtgaat ttcttgctta tttatgataa
    57181 aatgggagta tcgcaaaaaa tgactcatcg tattcaattt tgagtaaaac taggaggatc
    57241 ccatgtctac agaacatatg gaagaactaa atgaccagca gatcgttcgc cgtgaaaaaa
    57301 tggctgcgct ccgcgaacaa ggaatcgatc ctttcggaaa acgttttgaa cgtactgcaa
    57361 attcacaaga attaaaagat aaatatacca acctcgataa agaacaatta cacgataaaa
    57421 acgaaacagc tactatcgca ggacgcttgg taaccaaacg tggtaaagga aaagttggtt
    57481 ttgcccacct tcaagaccgc gaaggccaga ttcagatcta cgttcgtaag gatgctgtcg
    57541 gtgaagaaaa ctacgaaatc ttcaaaaaag cagaccttgg tgacttcctt ggtgtcgaag
    57601 gtgaagtgat gcgtacggat atgggagaac tctctatcaa ggcaacccac atcacacact
    57661 tgtctaaggc tcttcgtcct cttcctgaga aattccatgg tttgacagac gttgaaacaa
    57721 tttaccgtaa acgttacctt gacttgattt ctaatcgtga aagctttgaa cgctttgtca
    57781 ctcgttcaaa aatcatctct gaaatccgtc gttaccttga ccaaaaagga ttccttgaag
    57841 tggaaacacc tgttcttcat aatgaagccg gtggtgctgc tgcccgtcca tttatcaccc
    57901 accacaatgc ccaaaacatt gacatggtgc ttcgtatcgc gactgagctt cacttaaaac
    57961 gccttatcgt gggtggtatg gaacgtgtct atgaaattgg ccgtatcttc cgtaacgaag
    58021 gaatggacgc tactcataac cctgagttca cttctatcga agtttaccaa gcttatgcag
    58081 acttccaaga catcatggac ttgactgaag gcattatcca acacgctgct aaatcagtca
    58141 aaggtgatgg cccagtcaac taccaaggta ctgaaatcaa gattaacgaa ccatttaagc
    58201 gtgttcatat ggtggatgct atcagagaaa ttactggtgt cgatttctgg caagacatga
    58261 ctttggaaga agctaaagct atcgctgctg agaagaaagt tccagttgag aaacactaca
    58321 ctgaggttgg tcacatcatc aatgccttct ttgaagagtt tgttgaagaa actttaatcc
    58381 aaccaacctt tgtctatgga catccagtag ctgtatctcc actcgctaag aaaaatcctg
    58441 aagaccaacg ctttactgac cgtttcgagc tctttatcat gactaaggag tacggtaatg
    58501 cctttactga gttgaacgac ccaatcgacc aacttagccg ttttgaagcc caagctaaag
    58561 ccaaagaact tggtgatgat gaagcgacag gaatcgacta tgactacatt gaagctcttg
    58621 aatacggtat gccaccaaca ggtggtttgg gaatcggtat cgaccgtctc tgcatgctcc
    58681 tcactgatac aacaactatc cgtgatgtat tgctcttccc aacaatgaaa taaattctta
    58741 tcctctggct cttaccaggg gattttttga ttcaaaaaga gactgaattt aaggagaaaa
    58801 tgaagtgtag tatattgaag ttgaaatagt acactttgat ttctaagaca ttgttagaaa
    58861 ttggtttaaa ttccctaagc aatttgtgca tgttttattt cattttacga tagtacgctg
    58921 aaactgttca aaaagtacta gaaattgact tggattcccc aattgatttg ttcagattca
    58981 ctataaataa aaaattaata agtgggatag gaagttagcg tcaactagga tagtatcttg
    59041 cttaaacagt atatatggga ctgatataag tccataggtc ctattagagg atgttctggt
    59101 gtcttattca cttgtttttt tatagtatta gtagatagaa tccgcaaata aaaaagtagt
    59161 tcaaatgcct ggcagcaaaa aatctcaacg attcacttaa tatagactaa ggtttagagt
    59221 ttaaggaaag tagggctagt atattgtatg aaaaaataag atggttttta agcaaaactc
    59281 ttgaaaatga ttgaaaaaag agttattata gttgtgtagt aaaaagcaaa cgcttactta
    59341 atcaaggagg gcattttatg tcatacaaaa caagcaatgc agaaggtcat gtagacttca
    59401 tcaataccta tgatttggag ccaatggcgc aacaagttat tcctaaagca gcatttggct
    59461 atatcgctag tggggcggaa gatactttca ctttaagaga gaatatccgc gcctttaacc
    59521 acaaactcat cgttcctcat acactttgcg atgttgaaaa tccaagtaca gagattgaat
    59581 ttgcaggtga aaaactatct tcaccaatca ttatggcgcc tgttgcggcc cataaattag
    59641 cgaatgaaca gggggaagtg gcgactgcgc gtggtgtgca tgagtttggt tctctttata
    59701 caaccagttc ttactctact gttgaccttc cagagatttc tgaagccctt caagggacac
    59761 cccattggtt ccaattttac tttagtaagg atgacggtat caaccgccac atcatggatc
    59821 gtgtgaaggc tgaaggttat aaagcgattg tcttgacggc agatgctact gtagggggga
    59881 atcgtgaagt ggataagcgt aatggttttg tcttcccagt tggcatgccg attgttgaag
    59941 aatacctgcc agaaggtgct ggtaaatcaa tggactttgt ttacaaatca gctaaacaac
    60001 gcttgtctcc acgtgatgta gaatttatcg ctgaatactc aggacttcct gtgtatgtca
    60061 agggaccaca atgccgtgag gacgttgaac gttcgcttgc tgcaggagct tctggtatct
    60121 gggtaaccaa ccacggtggt cgtcaaatcg acggtggacc agctgccttt gactcgcttc
    60181 aagaagtggc agaagcagtt gataaacgtg tgccaattgt ctttgactct ggtgttcgtc
    60241 gtggtcaaca tgtctttaaa gccttggctt caggagcaga cttggtagct attggtcgcc
    60301 cggttatcta tggcttggct ctcggcggta gtgttggtgt gcgtcaagtc tttgaacact
    60361 tgaatgcgga attgaagaca gttatgcagt tgtctggaac tcagactatt gaagatgtta
    60421 aacacttcaa actccgtcac aacccataca acccaacttt cccagttgac cctcgtgact
    60481 taagattgta ttgataaaag agattgcctc cactgaatgt ggaggttttt cttcgtgtat
    60541 tggaagaata aatcgctatc gaaaaccata gtaaaatgga ataaaaacag gacaaatcaa
    60601 tcaggacagt taaatcgatt tttaacaatg ttttagaagt agatgtgtac tattctagtt
    60661 tcaatctact atatataaag atatttttcg gtcaaatatc ttgctagggc tttcattttt
    60721 tgctatactg gttcagtaat taaataatag agacatttgg ggtgctttaa gctgagatga
    60781 tacccattga acctgataca gttaagactg gcgaagggaa atgtgaaacg ttttttcgta
    60841 tgacgaaatg aacgatctaa tcttgtaagt caactctaat tcttgattgt aattggagtt
    60901 ttttgtttat ataaactgga taggcttgtt tagttagctc tctgcaaatg ctctcgatgt
    60961 gctttaaaag gatcaagtgg aattacgatt ttgcttgata tttcttggtg ttttattaat
    61021 tactaacaag taaagagagg agaagtaaat atggaaacac aagactatgc atttcagcca
    61081 ggtttgactg ttggagaatt attaaaaagc agtcagaagg attggcaggc tgcaatcaat
    61141 catcgttttg ttaaggaact ttttgcgggg acaattgaga ataaggtctt aaaggactat
    61201 ctgattcaag attatcactt ctttgatgcc ttcttatcca tgctgggtgc ctgcgtagct
    61261 cacgcagata aacttgaatc caagcttcgt tttgccaagc aactaggctt tcttgaagca
    61321 gatgaagatg gttatttcca aaaggctttt aaagagttaa aagtagcaga gaatgactat
    61381 ctagaagtga ctttgcaccc tgtaacaaaa gcctttcagg atctaatgta ttcggcagtg
    61441 gcttcatcag actatgccca tcttttgatt atgctggtca ttgcagaagg tctctattta
    61501 gactggggtt ctaaagattt ggctttacct gaagtctata ttcattcgga atggatcaat
    61561 ctccacagag gtcctttctt tgcagagtgg gttcaatttc tggttgacga actcaatcgt
    61621 gtcggtaaaa atcgagaaga tttgacagaa cttcagcaac gctggaatca agcagtcgct
    61681 ttagaattag ctttttttga tattggttac gatgtctaga gaaggctcat gatatttaca
    61741 aatttgatga ggtaagactc ttcgaaaatc tcttcaaacc acgtcagttc tatccacaac
    61801 ctcaaaacag tgttttgagc aacctgcggt tagcctccta gtttgctctt tgattttcat
    61861 tgagtataaa tcattcaatg taaatgaaaa aaattctcaa ctaaagataa atcgaaatta
    61921 gtagatcgta taaagtgaaa aggaaggatt tcaatatgac aagtttaaaa ttattaaaag
    61981 aaaaagcacc tttggtcatt tgcatcacca atgatgtagt aaaaaatttc acagcaaatg
    62041 gattagtagc actgggcgcc tcaccagcca tgagtgagtt tccagaagat ttagaggact
    62101 tattaaagta tgctggtggt ttattaataa acataggaac attgacagat gaaaattgga
    62161 aattatacca agctgctctg aaaattgcag agaaatataa tgtcccagca gttttagatc
    62221 ctgtagcctg tggagcagga gaatatagaa aaaaagtagc agatgattta atcaacaatt
    62281 acaaactagc tgcgattagg gggaatgctg gagagattgc ctctttagta ggaatagatg
    62341 tggcatctaa aggagtagat agtgcgggag tagataatat tgacgagatt gctctagcag
    62401 caaatgagaa gttcaatatt ccaatagtag taacaggtga agtggatgct attgcggtaa
    62461 atggagaagt ggtaacgatt cataatggta gtgccatgat gccaaaagtt attggaactg
    62521 gatgtttatt aggagctgta gtagcaagtt ttatcggact ggaaaaaggt caagaattaa
    62581 aatcattaga aacagcaatg ttagtttaca atatcgctgg agaaatggca gaaaaacgtc
    62641 caaatggaca tcttcctggg acatttaaag ttgaatttat aaatgcctta tacgagatta
    62701 cagatgaaga tgtaaaggaa ttcaaaagag tgaagtaaga tgtttcataa agaattacta
    62761 aaactatatt ttatttgtgg aacgactact tgccaaggaa aaaatctata tacagtcgtt
    62821 gaggaagcct taaaaggtgg tataacctta tttcaatttc gagaaaaagg tgagggtgct
    62881 ttagaaggct tagaaaaact tgagctagct atccaaatta aagaactttg taaaaaatac
    62941 aatgttccat ttatcgttaa tgacgatata gatttagcaa tggaaattga cgccgatggc
    63001 gtacatgtag gtcaagatga tattggtgtt gatgaaatta gaaaattgat gccagataaa
    63061 ataattggtc tttctataag aaacgaagaa gaatttcaac aatcaaaagt tgaatatgta
    63121 gattatgtag gtgttgggcc tgtatttgat acccagtcaa aagatgatgc tggtggtgct
    63181 ataggttatg aaggtcttga attgatgaga aaactattgc cacaaatgcc tttagttgca
    63241 attggtggga tacagacgaa acatattaaa gacattataa agaccaatat ggatggcgtt
    63301 tcaatcattt cggcaatatc gtatgcaaaa aatatagaaa aaactgttcg ggaaatgagt
    63361 gaacaataga tatctatcct tggattctaa gtaagtagga ttttcctttt ttaaaaagct
    63421 atgtccagat tcagcttgaa tggctaattc atcaaacagg tcatttgttt tttgcaaaag
    63481 aaagatctgc tgtctttgtg agtgcaaggc tgtatttcca gttcaatttc ttgaactagt
    63541 ataaaatgtg tgttataata atcacataat taaataataa agacatttgg ggtgctttag
    63601 gctgagatga tacccattga acctgataca gttaagactg gcgaagggaa atgtgaaatg
    63661 tttttttcgt atgtcgaaaa gaactgtcta atcttgtaag ctgaactcta attcgtatct
    63721 gtaattagag ttttttcttt tacgaaactg gatgaagatg atatagtaga ttgaaataag
    63781 atgcgaacaa atcaattaga aaattcaaat taatttctag aaatatttta gcattcttgt
    63841 gtactgttct aaattcagtc tgctatacaa agtatttagg tcggtcttct tgagtgcccc
    63901 acttttcttt aaaaaaagga gttttttatg ttgaaaaaat ggcagttaaa agatgttatc
    63961 ttgcttgctt tcttgtctat cttttttggt ggggttttcg ttggttcagg atatgtgtat
    64021 aatattctca gtctactctt aacacctctt ggtttgcagg cctttgccaa tgaaatcctc
    64081 ttcggtctct ggtgtatggc tgcgcccatt gctgccatct ttgttccgag agtcggaagt
    64141 gcaacgattg gagaagtgct agctgcgctt gctgaagtcc tttatggtag ccaatttggt
    64201 ctaggagctc ttttgtctgg cttggttcaa ggtttgggaa gtgaatttgg ttttatcgta
    64261 actaagaatc gctatgaaag ttggctctct ctaactgcta atagtattgg gattacgctt
    64321 gttagctttg tctatgaata cattaagtta ggttactacg ccttttccct tccgtttgtc
    64381 ctttccttac ttgtggtacg ttttatttct gtttatttct tctgtaccat cttggttcgt
    64441 gccattgtca aactctatca tcagtttgca actggaggaa aagcatagat gggtctggaa
    64501 ctacgagcga ttcagtcccc aatcttctct gagccgtttg attttacttt tcatgcgcaa
    64561 gcctttacct tgttagttgg gagtagtggg tctggaaaat ccagcctctt tcaaatgatt
    64621 gcccaagtca gttctcttcc ctatagtggt caagtcctga tagatgggag cgaggtcagt
    64681 caactttcta tcatcgcacg tgtccagaag gttggcattc tctttcaaaa tcccaatcat
    64741 caatttacca tggagagctt gtttgaggag ctgattttca ccttggagaa tatcggctat
    64801 caccttcagg aaattgattc taaaatagca gaggttgtcc agcaatgtcg ttgcgaggca
    64861 atcttacacc gtcccattca tcacctatca ggtggggaaa agcaaaaagc agcgctggct
    64921 gtcctctttg ccatgaatcc tagggtctat ctcttggatg aaccttttgc ttccattgac
    64981 cgcaagagca gaatcgagat attggagatt ctaaaagagt tggctcttga tggaaagaca
    65041 gttattttgt gcgaccatga tttatctgac tataaagcct atatcgacca tatggttgag
    65101 ctaagagacg gaaaactaag ggaagtgttt caaatccctt cctatgagat gacacaggtt
    65161 gcttcaaagg aagttgcttc tagcccggaa ctattccata tggatagaac gacttgtgag
    65221 tttggaaatc gccccctctt ttcaattgct gatttcacat tctatcaagg gatttcctgt
    65281 atcctgggtg acaatggtgt cgggaaatca accctctttc ggtctattct tcaatttcaa
    65341 aagtataagg ggcgcattgc atggaagggt tcggtcctga aaaagaaaaa gagtttgtat
    65401 cgtgatctga ctggtgttgt tcaggaagct gagaagcagt ttatccgagt cagtctgcga
    65461 gaggagcttc aattagatgg acctgattct gaaagaaatc agcggatttt tcaagcttta
    65521 cgatattttg atttggagca ggcagtcgat aagagtccct atcaattaag tggtggtcag
    65581 caaaaaattc ttcagctcct gaccatcttg accagtaagg cttccgtgat cttgctagat
    65641 gaaccttttg caggtttgga tgatagagcc tgccattatt tttgcaagtg gattgtggag
    65701 gagaggaatc aaggaagaag ttttctgctc attagtcatc gtttagaccc tttgatttct
    65761 gtggttgatt attggattga gatgactagt cagggtcttc ggcatgtgaa agaagtgacc
    65821 attaccaaac cacttacatc tcagagtagc aatacccaag gggaggtgag atagtatggt
    65881 caaagtagca acccagacac cgattatcag tctcttcttg ctgattttat ccttggaaac
    65941 atctttcatt ccttcgattg ctctgaatct ttcggtagtc gcattttgta ttctctttat
    66001 gctctattac cgtcgattta aaatgttagc ttggatgatc atacttgcca ttttaccatc
    66061 ttttgccaac tactgggcag ttcagttaca cggagatgct tcacaggcag tcatgcttgg
    66121 aacgagggcc tttgtgacag tttgtatcgg ccttgtcttt gtttcctcta tttcactaaa
    66181 agagcttctc ttgtacttgg ctcaaaaggg gctatcacgc tcttggtcct atgccttgat
    66241 tgtggtattc aattcttttc ctctcattca gcaagaaatc aagtccctca aagaagcttg
    66301 cctattacgt ggtcaagaac tacatttttg gtcgcccttg atttacagta aggttctgat
    66361 gacagtcttt aggtggcgcc atctttacct gagagctcta tctgctcacg gatatgacga
    66421 acatgcacag ttgaagaata gctatcggac tttttatatt cctaaaaaaa caaaattaat
    66481 ctacctgctt ttctttttat tgcttcaaac cagtctattt ttataaagga gttattatgg
    66541 aatttacaga tattgcgatg gaattatcca agaaagcttg gcaggcttcc tttcatcatc
    66601 cctttatttt acaattacaa gaggggaatt tagaacctgc cattttccgc tattacctga
    66661 ttcaggatgc ctactatctg aaggctttct cagaaatcta tcatcttttg gctgataaga
    66721 cttcaaacca agagatgaaa agactcttga aacaaaatgc tcagggttta gtggagggtg
    66781 agttatttat tcgccaacaa tttttcaagg aattggaaat cagcgaccag gaaatggagc
    66841 aacatccaat cgctccaacc tgttatcatt acatttctca tatttatagg caatttgcag
    66901 aaccgaactt agccatcgct tttgcaagct tgcttccttg tccttggtta taccatgata
    66961 taggcaaatc acttaatctt aaaccatcac caaatcctct ctatcaacaa tggattgaaa
    67021 cttatattac ggatgagtta gagcaacaga tcagagagga gggagcactg gtcaatcagc
    67081 tctatcgaga aagtgatgag acagataagc aaaaaatgct agatgccttc cacatcagtg
    67141 ttcatatgga agccaagttt tgggagatgg cttaccaaca ccagacatgg aagagcgatt
    67201 tacagtcttt agaaaaagga gaagaataga aacatgagaa agcaccaact acaagttcac
    67261 aaattgacca ttttatctat gatgattgcc cttgatgtag tccttacacc tatctttcgg
    67321 attgagggaa tggcaccgat gtccagtgta gtcaatattc tagcgggaat catgatggga
    67381 cctgtttatg ccttggctat ggctacagtg acagccttta tccgtatgac gactcaaggg
    67441 attccgcctt tagctctcac aggagcgact tttggagccc ttctagcagg tctcttttat
    67501 aagtacggtc gaaaatttta cttttctgcc ttgggagaaa ttgtgggaac aggtattatt
    67561 ggttctattg tttcctatcc tgttatggta ctctttacag gatcagctgc taagcttagc
    67621 tggtttatct acacgcctcg atttttcgga gcaaccttga ttggtacagc gatttccttt
    67681 attgcctttc gatttttaat caagcaggaa ttctttaaaa aagtgcaggg atatttcttt
    67741 agtgaaagga tagactgatg caggaattta caaatccctt tcctataggc tctagttccc
    67801 tcattcactg tatgaccaat gagatttctt gtgagatgct ggcaaatggg attttggctc
    67861 tgggatgcaa acctgtcatg gcagatgatc cccgtgaggt tcttgatttt actaagcaaa
    67921 gtcaggctct cttcatcaat ttggggcatt tgtcagctga gaaggagaaa gcaatccgta
    67981 tagcagcttc ttatgcagct caagtttgtc tcccaatggt agtagatgcg gttggcgtaa
    68041 cagcttcatc cattcgtaag agcttagtca gagacctttt agactataga cctacggtcc
    68101 ttaaaggaaa catgtcagaa attcgaagtc ttgttggatt aaagcaccac ggcgttgggg
    68161 tcgatgcgag tgctaaagat caagaaacgg aggatttgct tcaagtcttg aaagactggt
    68221 gtcagaccta tcatggtatg tctttcttag tcacaggtcc caaggacctc gtcgtttcga
    68281 aaaatcaggt cgctgtactg ggaaatggct gtgcagaatt agactggata acagggacag
    68341 gagatttggt tggagcctta acagccgttt ttctcagcca aggaaagact ggttttgaag
    68401 cttcttgctt agcagtctct tatctcaata tcgctgctga gaaaatagtt gttcaaggaa
    68461 tgggattgga agaatttcgt taccaagtac tcaatcagct ttcgctccta agaagagatg
    68521 aaaattggct agataccatc aaaggagagg tttatgaata gagaagcact tagactatat
    68581 ctggtaacca atcgctatca agattccgtg gaaagctttc ttgcaaaagt tgagacggcc
    68641 tgccgttcag gggttaccat agtccaattg cgagaaaaaa atctcacaac caatcaatat
    68701 tatcaactgg caaaacaagt caaggaaata acagatgctt atcaggtacc cttgataatc
    68761 gatgatcggt tggatgtttg tcttgcggtt gatgctgctg gtctgcatat tggagatgac
    68821 gaactcccag tttcggttgc ccgacaagtc ttgggtcctg aaaaaatcct cggtgtcacc
    68881 gctaaaacgg ttaaaagagc tctggaagcg gagaaatcag gtgcagatta cttggggaca
    68941 ggagccattt ttccgacaac caccaaggaa aatgcaccca tcaccctgat ttcaaccttg
    69001 aaaacaattt gccaaacggt tgccattcca atagttgcta ttggtggctt gacttcggag
    69061 aatattgatc agcttatggg cactggcata gctggtgtag ctgttgtacg tgatttgatg
    69121 caagcagaag atattgaggc aaaaacgcaa gcctttttaa caaagttgca tgacattctt
    69181 tcctaattaa tactcaatga aaatcaaaga gtaaactaga aagctagtcg caggttgctc
    69241 aaagtactgc cttgaggttt tagataagac tgacgaagtc agctcaaaac agtgttttga
    69301 agttgcagat ggaagctgac gtagtttgaa gagattttcg aagagtataa aaactctcta
    69361 attcccttaa agtgggaact agagagtttt ttttcttaat ctttaaaagt tgtatggttg
    69421 actggaccag aaccatgacc gagttgaggg gcatcttgaa tagcttttgt gataaaggcc
    69481 ttggccttat caactgcctg gtaaagactc ttgcccttgg ctagttcagc agtaatcact
    69541 gcagcaaagg tacatccagt accatgggtg tgacaggttt gaattcgtgg gctttcccag
    69601 acaaattgtt cattcttggt aaagaggaaa tctttagcac cacctttgag atgtccgcct
    69661 ttgataacca cagactgagg accaaattct tttaaaatca ggcgaccagc acgctgcatg
    69721 tcttcggggt catggattga aaaaccaaca atctcttctg cttcaggaag atttggcgta
    69781 ataatagttg ctagaggtag taagtttgtt ttgagatagt ctctagcact tgagtcaatc
    69841 aaggcatctc cacttgtagc aaccataaca ggatcaagga catagggaca atccagtttt
    69901 ttaagatagg gttggatgat ttccatgatt tcagtagtag ccaacattcc agtttttaca
    69961 gcctgaggtg gaatatcaga aaagacactc tccaattggg ctttcaacat ttgaggagaa
    70021 acgtgctcga ttagctgaac acctctggta ttttgagcga caagactggt tacaacagcc
    70081 attccataga catctctcgc ttggaatgac tttaaatctg ccataatgcc agcaccacca
    70141 ctagggtcag tccctgcaat ggtcaaagca acgggtaaat aagtcatcta aaacctccca
    70201 accaactgaa gtttgtattc ttaagaacaa gctagtctgt tttagaaagg caatagaaga
    70261 ctgctagtcc ccattatcat ttccacttcc atcagctagc attacctaga ttgagtcaga
    70321 gggatctaac agtcgttaat ctcagcttta cgcaccccta gtggttgttt tcttttttct
    70381 ttagtatagc atatttttat tgtttatata gtaatatttg actgcaaatc gtttttcaaa
    70441 tgattaaaat tctatttttc gaagaaaata ttatttctat aattgacaaa tgtagatttt
    70501 gggagtatac tgttgagtgt aattgacaaa tgtagatttt ggaggtatgg taatgcagat
    70561 ttcagatgca gaatggcagg tcatgaagat tatttggatg cagggggagc agaccagtac
    70621 agatttgatt agggttttgg cagagcggtt tgactggtcc aagtccacgg ttcagacact
    70681 tttggctcgt ctagttgaga aagagtgttt gactcggaaa aaagaaggca agtcctttgt
    70741 ttattcagcg cttttaactc tagaccaaag tcgggattta cttgtccaag atatcaaaga
    70801 caaggtttgt tcccgtagga ttaggaattt gttggctgac ttgattgtag aatgtgaatt
    70861 tactcagact gacttggaag acttggaagc tgtgatttca gagaagaaat caagtgctgt
    70921 aacagaagta agatgtaatt gtatgtaaag gagacgtcat gttaaatagt attgtaacca
    70981 ttatttgtat tgcccttatc gcgtttatct tgttttggtt tttcaaaaag cctgaaaaat
    71041 ctggacaaaa agcccagcaa aaaaacggat accaagagat tcgagtggaa gtcatgggag
    71101 gctatactcc tgagttgatt gtcctcaaga aatcagtgcc agcccgcatt gtctttgacc
    71161 gcaaggatcc ttcaccatgt ctggatcaaa ttgtttttcc agattttggt gtacatgcga
    71221 acctgccaat gggggaagag tatgtagtgg aaatcacgcc tgaacaggct ggagagttta
    71281 gctttgcttg tggtatgaac atgatgcacg gcaagatgat tgtagagtag gtggagacta
    71341 tgacagaaat tgtgaaagca agcttagaaa atggcattca aaaaatccgt atccaagctg
    71401 aaaaaggcta tcatccagcc catatccagc ttcaaaaggg aattccagct gagattacct
    71461 ttcatcgtgc tactccttca aactgttata aggaaattct gtttgaagaa gaaggtatct
    71521 tggaaccaat cggcgtagat gaggagaaag tcattcgttt tacacctcaa gaattagggc
    71581 gacatgaatt ttcttgtggc atgaagatgc aaaagggaag ctatacagtc gttgagaaga
    71641 ctcgaaaatc tctatctctc ctgcaacgtt tttggattac tagtatctgt actgtgcctc
    71701 ttgtgattct catgattggg atgttggcag gtagcattag tcatcaagtc atgcattggg
    71761 gaacctttct agcaacaacg cctattatgt tagttgcagg taagccatat atccagagtg
    71821 cttgggccag ttttaaaaag cacaatgcca acatggatac cttggttgcg ctgggaactc
    71881 tagtggctta tttctatagc ctagttgctc tctttgctgg tctccctgtt tacttcgaaa
    71941 gtgctggatt tatcctcttt ttcgttcttt tgggagcagt ttttgaggaa aaaatgagaa
    72001 aaaatacgtc ccaagctgtg gagaaattac tggacttgca agctaaaacc gcagaagtct
    72061 tgagtgatga tagttatgtc caagttcctt tggaacaagt caaggtaggc gacctgattc
    72121 gagtgcgtcc cggtgaaaag attgctgttg atggtgtcgt agtagaaggt gtctctagta
    72181 ttgacgaatc catggtgaca ggtgagagtc tgcctgtgga caagacagtt ggagatactg
    72241 tcattggctc aaccatcaat catagtggaa cgcttgtctt tagagcagaa aaagttggct
    72301 cagagactgt tttggctcag attgtggatt ttgtgaagaa agctcagaca agtcgtgcgc
    72361 cgattcagga cttgacggat aagatttcag ggatttttgt cccagtagtt gtcattttag
    72421 gaatcatgac cttttgggtt tggttcgtct tgctcaggga tagtgtggtt gtgcttggag
    72481 ctagctttgt gtcctctctt ctctacggag tggcggtttt gattatcgcc tgtccttgtg
    72541 ccttgggact tgcaacaccg acagccctta tggtggggac aggacgtagt gccaagatgg
    72601 gggttctcct caaaaatgga actgtcttac aggaaatcca gaaagttcaa acccttgtct
    72661 ttgataagac cgggactttg acggaaggga aacctgtggt aacagatgtc atcggcgacg
    72721 aagtagaagt gtttggattg gcagcctcct tggaagatgc ttctcaacac ccactggctg
    72781 aggctatcgt taagcgagcg agtgaagctg gacttgagtt tcaaactgtt gaaaattttc
    72841 aggccttgca cgggaaaggt gtttcagggc gaatcaatgg aaaacaagtt ttacttggaa
    72901 atgctaaaat gctggatggc atggatattt ctaatactta tcaagataaa ctagaagaac
    72961 tagaaaaaga agctaagaca gttgtgttct tagctgttga caatgaaatc aaaggcttgc
    73021 ttgctttgca agatattcct aagaaaaatg ctaagctagc catcagtcag ctaaaaaaac
    73081 gtggtctcgg gacagtcatg ctgacaggag acaatgctgg tgtggcgcgt gctattgcag
    73141 atcaaatcgg aattgaagag gtcattgcag gcgtcttgcc agaagaaaaa gcccatgaaa
    73201 tccataaact gcaacagtca ggtaaagtag cctttgttgg tgatgggatc aatgacgctc
    73261 ctgcccttag tgtagcagat gtggggattg ctatgggagc tggaacagat atcgccatcg
    73321 agtcagcaga tttggtgttg acaaccaata atcttttagg agtggtgcgt gccttcgaca
    73381 tgagtaagaa aacctttcat cgtattctgc tcaatctttt ctgggctttt atctacaatg
    73441 ttgtcggaat tccgattgca gcaggagtct tttcaggtgt tggactggct ctcaacccag
    73501 aattggcagg tctagccatg gcctttagtt ctgtatctgt tctgaccagt tcccttctgt
    73561 taaattttag taaaatagac taaaagtaat gactagattt ttttgttata aaacagaaat
    73621 gacgacactt tcaaaactgt attagaatag agaatagaga gttttgagca gatttttaga
    73681 aaagtcagca taaatatgat acagtggaat agtaaaaaat tggagaacgt ttccaattct
    73741 atgtaatcgt attctccaag tctaaaaaaa ttgaaggaga gttatcatta tgactcaagg
    73801 gaaaattact gcatctgcag caatgcttaa cgtattgaaa acatggggcg tagatacaat
    73861 ctacggtatc ccatcaggaa cactcagctc attgatggac gctttggctg aagacaaaga
    73921 tatccgcttc ttacaagttc gccacgaaga gacaggtgct cttgcagcgg ttatgcaagc
    73981 taaattcggc ggctcaatcg gggttgcagt tggttcaggt ggtccaggtg cgactcactt
    74041 gattaacggt gtttacgatg cagctatgga taacactcca ttcctagcaa tccttggatc
    74101 acgtccagtt aacgaattga acatggatgc tttccaagag cttaaccaaa acccaatgta
    74161 caacggtatc gctgtttaca acaaacgtgt agcttacgct gagcaattgc caaaagtaat
    74221 tgacgaagcc tgccgtgctg cagtttctaa aaaaggtcca gctgttgttg aaattccagt
    74281 aaacttcggt ttccaagaaa tcgacgaaaa ctcatactac ggttcaggtt catacgaacg
    74341 ctcattcatc gctcctgctt tgaacgaagt tgaaatcgac aaagctgttg aaatcttgaa
    74401 caatgctgaa cgcccagtta tctatgctgg atttggtggt gttaaagctg gtgaagtgat
    74461 tactgaattg tcacgtaaaa tcaaagcacc aatcatcaca actggtaaaa actttgaagc
    74521 tttcgaatgg aactatgaag gtttgacagg ttctgcttat cgtgttggtt ggaaaccagc
    74581 caacgaagtg gtctttgaag cagacacagt tcttttcctt ggttcaaact tcccatttgc
    74641 tgaagtttac gaagcattca agaacactga aaaattcatc caagttgata tcgaccctta
    74701 caaacttggt aaacgtcatg cccttgacgc ttcaatcctt ggtgatgctg gtcaagcagc
    74761 taaagctatc cttgacaaag taaacccagt tgaatcaact ccatggtggc gtgcaaacgt
    74821 taagaacaac caaaactggc gtgattacat gaacaaactc gaaggtaaaa ctgagggtga
    74881 attgcaattg tatcaagttt acaatgcaat caacaaacat gctgatcaag acgctatcta
    74941 ctcaatcgac gtaggtaaca ctactcaaac atctactcgt caccttcaca tgacacctaa
    75001 gaacatgtgg cgtacatctc cactctttgc gacaatgggt attgcccttc ctggtggtat
    75061 cgctgctaag aaagacaatc cagatcgcca agtatggaac atcatgggtg acggagcatt
    75121 caacatgtgc tacccagacg ttatcacaaa cgttcaatac gaccttccag ttatcaacct
    75181 tgtcttctca aatgctgagt acggcttcat caagaacaaa tacgaagata caaacaaaca
    75241 cttgtttggt gtagacttca caaacgctga ctacgctaaa attgcggaag ctcaaggagc
    75301 tgttggattc acagttgacc gtatcgaaga catcgatgca gttgttgcag aagctgttaa
    75361 attgaacaaa gaaggtaaaa ctgttgtcat cgatgctcgc atcactcaac accgtccact
    75421 tccagtagaa gtacttgaat tggatccaaa acttcactca gaagaagcta tcaaagcctt
    75481 caaggaaaaa tacgaagcag aagaactcgt accattccgt ctcttcttgg aagaagaagg
    75541 attgcaatca cgcgcaatta aataattcct ctcgccgaaa atcaaatatg aaacttgtag
    75601 actttattct ttgattttca tagagcagaa ctttaaaaga tcggagcaat ccggtctttt
    75661 tatggaggag agtaaatgaa tttaaatcaa ttagatatta tcgtttcaaa tgttccccaa
    75721 gtctgtgctg acttggagca tattttggat aaaaaggcag attatgctga tgatggtttt
    75781 gctcagttta cgattggtag tcactgcctt atgttgtcac aaaatcattt ggttcctttg
    75841 gaaaactttc agtcaggaat cattattcat atcgaggttg aggatgtaga ccagaactac
    75901 aaacggttga acgagcttgg tataaaggtt ttacacggac caactgtaac cgattgggga
    75961 acagagtcct tattagttca aggacctgct ggtctagtgc ttgattttta tcgtatgaaa
    76021 taggtcgcgt agtcatttga cgttaacctt agctatttt
//
LOCUS       DBERYS010000005        75171 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.005, whole genome shotgun sequence.
ACCESSION   DBERYS010000005 DBERYS010000000
VERSION     DBERYS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 75171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.005"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(126..995)
                     /gene="thrB"
                     /locus_tag="ACL8LA_000370"
     CDS             complement(126..995)
                     /gene="thrB"
                     /locus_tag="ACL8LA_000370"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGS1812192.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(997..2283)
                     /locus_tag="ACL8LA_000371"
     CDS             complement(997..2283)
                     /locus_tag="ACL8LA_000371"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGS1812193.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(2434..3171)
                     /gene="mecA"
                     /locus_tag="ACL8LA_000372"
     CDS             complement(2434..3171)
                     /gene="mecA"
                     /locus_tag="ACL8LA_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGS1812194.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(3371..4405)
                     /locus_tag="ACL8LA_000373"
     CDS             complement(3371..4405)
                     /locus_tag="ACL8LA_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS1812195.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(4434..5867)
                     /locus_tag="ACL8LA_000374"
     CDS             complement(4434..5867)
                     /locus_tag="ACL8LA_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGS1812196.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(5883..6869)
                     /locus_tag="ACL8LA_000375"
     CDS             complement(5883..6869)
                     /locus_tag="ACL8LA_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1812197.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(6871..7974)
                     /locus_tag="ACL8LA_000376"
     CDS             complement(6871..7974)
                     /locus_tag="ACL8LA_000376"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1812198.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(7964..8809)
                     /locus_tag="ACL8LA_000377"
     CDS             complement(7964..8809)
                     /locus_tag="ACL8LA_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1812199.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(9088..10362)
                     /gene="psr"
                     /locus_tag="ACL8LA_000378"
     CDS             complement(9088..10362)
                     /gene="psr"
                     /locus_tag="ACL8LA_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGS1812200.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSPLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(10359..11207)
                     /gene="pheA"
                     /locus_tag="ACL8LA_000379"
     CDS             complement(10359..11207)
                     /gene="pheA"
                     /locus_tag="ACL8LA_000379"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS1812201.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(11204..11680)
                     /locus_tag="ACL8LA_000380"
     CDS             complement(11204..11680)
                     /locus_tag="ACL8LA_000380"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGS1812202.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(11673..12956)
                     /gene="aroA"
                     /locus_tag="ACL8LA_000381"
     CDS             complement(11673..12956)
                     /gene="aroA"
                     /locus_tag="ACL8LA_000381"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGS1812203.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(13048..13386)
                     /locus_tag="ACL8LA_000382"
     CDS             complement(13048..13386)
                     /locus_tag="ACL8LA_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGS1812204.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(13397..14500)
                     /locus_tag="ACL8LA_000383"
     CDS             complement(13397..14500)
                     /locus_tag="ACL8LA_000383"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGS1812205.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(14510..15676)
                     /gene="aroC"
                     /locus_tag="ACL8LA_000384"
     CDS             complement(14510..15676)
                     /gene="aroC"
                     /locus_tag="ACL8LA_000384"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGS1812206.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(15686..16753)
                     /gene="aroB"
                     /locus_tag="ACL8LA_000385"
     CDS             complement(15686..16753)
                     /gene="aroB"
                     /locus_tag="ACL8LA_000385"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGS1812207.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            complement(16772..17626)
                     /locus_tag="ACL8LA_000386"
     CDS             complement(16772..17626)
                     /locus_tag="ACL8LA_000386"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGS1812208.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALENVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(17616..18293)
                     /gene="aroD"
                     /locus_tag="ACL8LA_000387"
     CDS             complement(17616..18293)
                     /gene="aroD"
                     /locus_tag="ACL8LA_000387"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGS1812209.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(18290..19453)
                     /locus_tag="ACL8LA_000388"
     CDS             complement(18290..19453)
                     /locus_tag="ACL8LA_000388"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGS1812210.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(20138..21772)
                     /locus_tag="ACL8LA_000389"
     CDS             complement(20138..21772)
                     /locus_tag="ACL8LA_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812211.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(21753..22502)
                     /locus_tag="ACL8LA_000390"
     CDS             complement(21753..22502)
                     /locus_tag="ACL8LA_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812212.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            22681..24135
                     /locus_tag="ACL8LA_000391"
     CDS             22681..24135
                     /locus_tag="ACL8LA_000391"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGS1812213.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(24225..26843)
                     /gene="alaS"
                     /locus_tag="ACL8LA_000392"
     CDS             complement(24225..26843)
                     /gene="alaS"
                     /locus_tag="ACL8LA_000392"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGS1812214.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPCITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(26865..27350)
                     /locus_tag="ACL8LA_000393"
     CDS             complement(26865..27350)
                     /locus_tag="ACL8LA_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGS1812215.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(27625..27864)
                     /locus_tag="ACL8LA_000394"
     CDS             complement(27625..27864)
                     /locus_tag="ACL8LA_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812216.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(28125..29195)
                     /locus_tag="ACL8LA_000395"
     CDS             complement(28125..29195)
                     /locus_tag="ACL8LA_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812217.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(29192..29965)
                     /locus_tag="ACL8LA_000396"
     CDS             complement(29192..29965)
                     /locus_tag="ACL8LA_000396"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812218.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(29962..30768)
                     /locus_tag="ACL8LA_000397"
     CDS             complement(29962..30768)
                     /locus_tag="ACL8LA_000397"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812219.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(30749..31906)
                     /locus_tag="ACL8LA_000398"
     CDS             complement(30749..31906)
                     /locus_tag="ACL8LA_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812220.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(32066..32971)
                     /gene="murB"
                     /locus_tag="ACL8LA_000399"
     CDS             complement(32066..32971)
                     /gene="murB"
                     /locus_tag="ACL8LA_000399"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGS1812221.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(33083..33835)
                     /locus_tag="ACL8LA_000400"
     CDS             complement(33083..33835)
                     /locus_tag="ACL8LA_000400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS1812222.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(33845..34576)
                     /gene="budA"
                     /locus_tag="ACL8LA_000401"
     CDS             complement(33845..34576)
                     /gene="budA"
                     /locus_tag="ACL8LA_000401"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGS1812223.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            34718..35989
                     /locus_tag="ACL8LA_000402"
     CDS             34718..35989
                     /locus_tag="ACL8LA_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGS1812224.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(36009..36824)
                     /locus_tag="ACL8LA_000403"
     CDS             complement(36009..36824)
                     /locus_tag="ACL8LA_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812225.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(36981..37634)
                     /gene="phoU"
                     /locus_tag="ACL8LA_000404"
     CDS             complement(36981..37634)
                     /gene="phoU"
                     /locus_tag="ACL8LA_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS1812226.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(37646..38404)
                     /gene="pstB"
                     /locus_tag="ACL8LA_000405"
     CDS             complement(37646..38404)
                     /gene="pstB"
                     /locus_tag="ACL8LA_000405"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1812227.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(38417..39220)
                     /gene="pstB"
                     /locus_tag="ACL8LA_000406"
     CDS             complement(38417..39220)
                     /gene="pstB"
                     /locus_tag="ACL8LA_000406"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1812228.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(39231..40115)
                     /gene="pstA"
                     /locus_tag="ACL8LA_000407"
     CDS             complement(39231..40115)
                     /gene="pstA"
                     /locus_tag="ACL8LA_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS1812229.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(40105..41022)
                     /gene="pstC"
                     /locus_tag="ACL8LA_000408"
     CDS             complement(40105..41022)
                     /gene="pstC"
                     /locus_tag="ACL8LA_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS1812230.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFITEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(41022..41900)
                     /gene="pstS"
                     /locus_tag="ACL8LA_000409"
     CDS             complement(41022..41900)
                     /gene="pstS"
                     /locus_tag="ACL8LA_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGS1812231.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(42474..43778)
                     /locus_tag="ACL8LA_000410"
     CDS             complement(42474..43778)
                     /locus_tag="ACL8LA_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGS1812232.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(43779..44552)
                     /locus_tag="ACL8LA_000411"
     CDS             complement(43779..44552)
                     /locus_tag="ACL8LA_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGS1812233.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(44542..44820)
                     /locus_tag="ACL8LA_000412"
     CDS             complement(44542..44820)
                     /locus_tag="ACL8LA_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGS1812234.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(44820..45221)
                     /locus_tag="ACL8LA_000413"
     CDS             complement(44820..45221)
                     /locus_tag="ACL8LA_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGS1812235.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(45431..46048)
                     /locus_tag="ACL8LA_000414"
     CDS             complement(45431..46048)
                     /locus_tag="ACL8LA_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGS1812236.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(46038..46811)
                     /locus_tag="ACL8LA_000415"
     CDS             complement(46038..46811)
                     /locus_tag="ACL8LA_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGS1812237.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(46822..47559)
                     /locus_tag="ACL8LA_000416"
     CDS             complement(46822..47559)
                     /locus_tag="ACL8LA_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGS1812238.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(47564..48694)
                     /gene="hemW"
                     /locus_tag="ACL8LA_000417"
     CDS             complement(47564..48694)
                     /gene="hemW"
                     /locus_tag="ACL8LA_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGS1812239.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(48778..49167)
                     /locus_tag="ACL8LA_000418"
     CDS             complement(48778..49167)
                     /locus_tag="ACL8LA_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGS1812240.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(49140..49566)
                     /locus_tag="ACL8LA_000419"
                     /pseudo
     CDS             complement(49140..49566)
                     /locus_tag="ACL8LA_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            complement(49567..50355)
                     /gene="lgt"
                     /locus_tag="ACL8LA_000420"
     CDS             complement(49567..50355)
                     /gene="lgt"
                     /locus_tag="ACL8LA_000420"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGS1812241.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            complement(50348..51283)
                     /gene="hprK"
                     /locus_tag="ACL8LA_000421"
     CDS             complement(50348..51283)
                     /gene="hprK"
                     /locus_tag="ACL8LA_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGS1812242.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(51413..51589)
                     /gene="rpsU"
                     /locus_tag="ACL8LA_000422"
     CDS             complement(51413..51589)
                     /gene="rpsU"
                     /locus_tag="ACL8LA_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGS1812243.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(51729..52436)
                     /locus_tag="ACL8LA_000423"
     CDS             complement(51729..52436)
                     /locus_tag="ACL8LA_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGS1812244.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            52587..53615
                     /gene="queA"
                     /locus_tag="ACL8LA_000424"
     CDS             52587..53615
                     /gene="queA"
                     /locus_tag="ACL8LA_000424"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGS1812245.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFY
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(54276..54665)
                     /locus_tag="ACL8LA_000425"
     CDS             complement(54276..54665)
                     /locus_tag="ACL8LA_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS1812246.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(54719..55426)
                     /locus_tag="ACL8LA_000426"
     CDS             complement(54719..55426)
                     /locus_tag="ACL8LA_000426"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS1812247.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKEATDTVIKVPAKSKVEEVFVPFATKYEADNDLSAGQEQEITLGKNGKIVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(56126..56677)
                     /locus_tag="ACL8LA_000427"
     CDS             complement(56126..56677)
                     /locus_tag="ACL8LA_000427"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812248.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(57027..57851)
                     /gene="nadE"
                     /locus_tag="ACL8LA_000428"
     CDS             complement(57027..57851)
                     /gene="nadE"
                     /locus_tag="ACL8LA_000428"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGS1812249.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(57848..59308)
                     /locus_tag="ACL8LA_000429"
     CDS             complement(57848..59308)
                     /locus_tag="ACL8LA_000429"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGS1812250.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(59424..59912)
                     /locus_tag="ACL8LA_000430"
     CDS             complement(59424..59912)
                     /locus_tag="ACL8LA_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGS1812251.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(59902..60111)
                     /locus_tag="ACL8LA_000431"
     CDS             complement(59902..60111)
                     /locus_tag="ACL8LA_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1812252.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(60497..60967)
                     /locus_tag="ACL8LA_000432"
                     /pseudo
     CDS             complement(60497..60967)
                     /locus_tag="ACL8LA_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60992..61863)
                     /locus_tag="ACL8LA_000433"
                     /pseudo
     CDS             complement(60992..61863)
                     /locus_tag="ACL8LA_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(62220..62948)
                     /locus_tag="ACL8LA_000434"
                     /pseudo
     CDS             complement(62220..62948)
                     /locus_tag="ACL8LA_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
     gene            complement(63208..63519)
                     /locus_tag="ACL8LA_000435"
     CDS             complement(63208..63519)
                     /locus_tag="ACL8LA_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HGS1812253.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(63534..64820)
                     /locus_tag="ACL8LA_000436"
     CDS             complement(63534..64820)
                     /locus_tag="ACL8LA_000436"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGS1812254.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            65417..66001
                     /locus_tag="ACL8LA_000437"
                     /pseudo
     CDS             65417..66001
                     /locus_tag="ACL8LA_000437"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            65998..67188
                     /locus_tag="ACL8LA_000438"
     CDS             65998..67188
                     /locus_tag="ACL8LA_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGS1812255.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(67703..68695)
                     /locus_tag="ACL8LA_000439"
     CDS             complement(67703..68695)
                     /locus_tag="ACL8LA_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1812256.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            68855..70609
                     /locus_tag="ACL8LA_000440"
     CDS             68855..70609
                     /locus_tag="ACL8LA_000440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812257.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            70593..72314
                     /locus_tag="ACL8LA_000441"
     CDS             70593..72314
                     /locus_tag="ACL8LA_000441"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812258.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            72325..72909
                     /locus_tag="ACL8LA_000442"
     CDS             72325..72909
                     /locus_tag="ACL8LA_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS1812259.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            72909..73604
                     /locus_tag="ACL8LA_000443"
     CDS             72909..73604
                     /locus_tag="ACL8LA_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1812260.1"
                     /translation="MSTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            73592..74956
                     /locus_tag="ACL8LA_000444"
     CDS             73592..74956
                     /locus_tag="ACL8LA_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812261.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSINVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT        23139 a        16041 c        13659 g        22332 t
ORIGIN      
        1 tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa
       61 cttcttttca caaaaaggat atgaaaccct ctggttaaga gtttccccat cctatcttct
      121 attctttatt ttgcttctac acggacacct tgggtatcaa ctctcaagtc atgcagtttt
      181 cctttgaaag gttgcttttc caattctgcc ttaattgttg gcatcttgtc atgagaagcc
      241 agaaccataa ctgtcggccc agcaccagaa aggtaggttg cataggcccc attttctttg
      301 gtcacttgct taatcatcgc aaattctctt accaagtcct gacgataacg ctcatggaag
      361 aggtctccct cgattgcttg cccagcggtc accatgtctc ctgccaacaa ggcagcaacc
      421 gctacattgg cgatagaact tgcagcaaca gcttccttat aagacaattt tttaggcaag
      481 acactacggc tgtcgcgagt acgtaattca tagtttggaa tgtaagctag aaaatcacac
      541 tctggaaagt ctgctacgat agcagagact tgcccttcaa cagaacttgc aataacgaga
      601 ttaccataaa tggctggagc cacattgtca ggatgccctt caatcttggt cgctaactgc
      661 aatttttcat ggtctgataa gttgagttga cccagttggt tggctagttc aatcccagca
      721 acgataaccg agctggaaga acccaaaccg cgcgccaaag ggacatcact ggtcattttc
      781 aagcgtcttg gttgcaagtc tggtacaatt tgcaaagcga ttttgagcaa gagattacgc
      841 tcatcatgtg gaatccattt gccaatctgg tgttcaatca gccactcatc tcgttcttcg
      901 cagacctcaa tttgaagata cttggttaca gctacaccga ccgagtcaaa acctggcccg
      961 atattggcac tggttgcagg tacaataatc ttcatcttat tctcctagca ccttgaaggt
     1021 attcaagagg tcgaattctg aaaccttctt caattcagct gagacatttt caagctgggc
     1081 tttattaatc ttgtgtgtga tgataacgac acgcgccttg tcaccctctt tgccatcttg
     1141 aaggatttgc ttaaaggaaa tatcttgagc attgaagatt tcagccaact tcaagacctg
     1201 accttttgag tctggagcca agattgagaa atagtagttt gctttgacat cttcaggatt
     1261 tgccaagacc aagtcacggc tatattcgtt gaagtctttg ccaatggtac catcattcaa
     1321 acgacgaacg atacggacaa tatcagctac aacacttgtt gcagttggtt tttgacccgc
     1381 acctggtccg tagtacatag actcaccaat accgatagat tctacaaaga cagcgttcat
     1441 tacgccattc acactagcaa gtgggtgcgc tttaggtagg aaggttggag tcacttctgc
     1501 agcaatacct gaagaagttt cctcaataga accaaccaat ttcactacgt aaccaaactc
     1561 ttgagctaca gctacgtctt ctggtgtgat attgcggatt cccttgtggg ctacatcatc
     1621 aaaggcaatc ttcatgccaa aggcaaattg gctcaaaata accatcttgt aggctgcatc
     1681 aatcccatct acgtcattcg tcggatcgct ttctgcaaat cctagacgtt gtgcttccgc
     1741 aagagcatca tcgtaagacc agccttcttc caccatcttg gtcaccatga agttggaagt
     1801 tccgttgact actccaagca cgcgcgtaat tttatcagaa gccaaggaat ttgctaaagt
     1861 acgaagaatt ggaatcccac cagcaactgc tgcttcgtag taaagtgcta ccttgttagc
     1921 ttgagcgatt tctagcaatt ctgcgccatg gacagctaaa aggtccttgt tagcagtaac
     1981 aacgtgtttt ccagcttcca aggcacgagt gataaaggtt ttagcaggct caatacgccc
     2041 catcaattcc actacgatag taatatcctg gtctgataaa atatcatcca cattggttac
     2101 aaagttaaag tcattccctg ctgcaagcaa gcgatttttt tcatcttcat ccttgaccaa
     2161 taccttagca actttgatat ctgaatgtgc tgattgattg atttttcctc cattttcctt
     2221 taggaggaaa ggcacaccac ttgcaacggt accaaatcct agtaaagcaa ttttaactgt
     2281 catctttatc tcctcgaaat tttctaatat agccattata acagaatttt gtgaaaattc
     2341 ctattatagt aaatcactat ttcagtataa aaagaaaaaa cgaatcagac gattcgctct
     2401 tcttaaaatc tgaaaatagc tttccagaaa ggattagccg attttttgca gattgagcac
     2461 tgcatcgtga ctcatcaaga cttgaccata ctcttgtaag actgagcgac tgatatcact
     2521 atcgtctgca aactcgcgca tacgggccaa cagccaagct ggatatgggc ttggatgatt
     2581 ttcaatatcc actaaaatgg tcaaataata gcgctcgttc attttgtaga gttcagaagt
     2641 ttccatttca aaagtcactg tcttggcaaa aactaccaag tcagccaact tagcaaaaga
     2701 aaggatgtag tagatgtaag gttctttctt actctcagct tcttgttcag cctgctcttg
     2761 ctcttcttcc ttggcttcaa cttgctcaag agattgaatg gcttcgatat catccttggt
     2821 tttgtctgcg atgctttttt ccagggtttt gataaattca tctggagaca tttgagccaa
     2881 ttcttccata tctggcaaat ccgataagtc ttcaaaatct agattttggt caatctttga
     2941 cttggtcaca aagacatcta ccttatcagg ttttggagtc acacggaagc tcaacatgcc
     3001 tgtatccaga aagctatcag gcatctctag ttcatccaag atagcataaa agaactcttc
     3061 tgttttttct tgaggaacga gaaagtcagc aatctccatt ccacgatcca tcaaatcctc
     3121 taaagacatc gtgattttta aagttgtatc actaatttgt ttcattttca ttgctagtaa
     3181 cctcatactt tcagttctat ctattatact agatttttac gattttatca aaagaaggct
     3241 cctctatacg gatagatttt ccctagggtc tttctatagg agactccaaa agaaaatttc
     3301 tgcagacaga tagaaaaagc cttcaaaatc agctcttagc cgattttgaa gaccttatac
     3361 atcagaatac ttataattta aaggttgcta caccaaggat agaacgattt aagtttctga
     3421 gaatttgaag actttgctca aatttcttat aacgagtcac tccgtactct tcaacaagaa
     3481 ggactgtatc tctttccaaa agagatgata catcctgtaa atctacaaaa tgcattcctt
     3541 ttaaagcttc ttgactctgt ttcaatttat ctaggatagc tttatttgag ctaacgatgg
     3601 tcaattcctg tccagtattt ttgtatgaca aaacatctgc taggttagca attgttgtaa
     3661 tctctgttac aaaatcaatt tgatactgag aaaaatcacc tactctattg attgttggat
     3721 taaagagata aactaacaca tttcccatca caaccaaaat cacacaaacc actccaataa
     3781 caactaaacg aagaatcaga tttttcacat ttaagccaag cgctgtttca ccatttgcgt
     3841 tcaattcttt agagttgatg gtttccagtt tttcaatttt cacatttgca taggcatgtt
     3901 taaatttctc aatcaaccca tcaatttttt tctctaacaa gtcattggca tctttacttg
     3961 atgtcaaaat tttcacacca acccctgcat cgtcaatcat atagtagacg gtcaattttt
     4021 tccaccaata gtcattcgtt gaatttttca aggttgtttc tgtcgtgtct aattcactgg
     4081 caattttttt caactcactg ggttctacat cattgaaaag ataagctcca ttcaaattac
     4141 catcaatcaa tttcccataa aaatcactat aaccaccaat ttgatgattc aaaatcgttt
     4201 tggccgactc ttttggagga gtgattttat agataagata agttgaataa cttgttgtat
     4261 ctttgacagt gtttttattc ctaactgctt taattgtaaa tggtacagca atgagagcaa
     4321 ataaagcgat gagagctaaa atatttgctt ttcgcttttt ataaagattt gcaaacaaat
     4381 cagctactga ataatgttca aacatgattt ttttctcctt tgtttagtag atactagttt
     4441 tcctttgtaa gcatttttgc tacaaatata atcacaagaa caattcccca gaattgcatt
     4501 gtaaataaat tgaagaaact ttctgaaaag ctgcttcttg gcataaagaa tagattattc
     4561 aagatgagta gggataaagc aaataggatt gtccttgagc gataggctac ttgcagcatg
     4621 gctataaata atacgccgag taagaaacta agcagaaaga ctccaatcat accatagtcg
     4681 gtatacaact ccatgatata actacttccg ataccatgcc ctttcaagta ttccttgttc
     4741 aagacaagat aggatagatt gtgggcataa ctattactat caatagctag ttccacacta
     4801 ttggttgtat gttcaaaggc ttttcctccg aaaatggctc ccaaactccc ccttgcaaaa
     4861 taatcaagaa caggaccaaa agtaaaatta cggaaatctc ggtaagggag gctactgtta
     4921 aatagaaaac ctcgagccag aacaccaaaa ctagtccctt gtttatagat aaagtcaagt
     4981 aagatatccc agaaacctgt atgagaaact tggacattat cccgtacata attgagtact
     5041 cccatcgcta acatgagaat aggagaacct acaaaaatcg ctaacttttc tttaaaccca
     5101 atccattttc ctttttcagt ttgctcccgc ataaagtaat aaacaaaagc aaataaaata
     5161 cttaaaataa agggatttcg tgtcccaatt gccaaatgaa tagtattagc tgcaataaag
     5221 gagacaagca ctgctgtggc ctgcaatttc tttggcttgg ttgccagata catacacatt
     5281 gcatagaccg taaaggtaga caaaatgtag gtaaaataag gcagtttact ttcaaaattt
     5341 gcatagtagg catagtagga agtctgcaaa cgatacaaga gccgttcaaa taaccgaatg
     5401 aaatagaaag gataggttag aagaaaaact cctagtgata caaagcgtaa ccgcttgata
     5461 taaacctctt ttagagaatt tcctatattt gctactttta ttttcttcct agctatgaag
     5521 taacgagcca gaatgcctcc tgtggtcaag cccagaatcg aaaccatgac aactataaag
     5581 gcaaaacgat aggctattgg atgataggta tccaaagcac catccctaaa ataatcaatg
     5641 gtcggtcttg ataccagaaa tacaaaaatg gttaaataga aaataaaatg gattaagtaa
     5701 tacttgatat cattccaaca agcaattaag ctactaacca acaagaacaa taaagtagaa
     5761 agtaagctaa cattattatt attaaacaga tacacaattc cacttactag cgtcaaggca
     5821 taactgacta tggtcaaact aaataataat cgtttcccat caatcacttg gtcacccccg
     5881 ttctaatgta attttttaga tttttcaata tttttcagta ataagaatcg atataaggaa
     5941 atatttatga atagggccaa agcactaatt cttctcccct tacggaaaat tggattccta
     6001 gaaatagcaa aggcatggcc ttttaaaaaa cgatgaatct gagaataggc ttcaaactgt
     6061 ttatactgat catctagcaa catcttatcc agaataaaga agtgggcata ggccaatctg
     6121 aaaaaagcga cctctttcaa gtcaggatag tttttcacaa cttcattata aaacttttgg
     6181 tagatatcaa tataggctaa atccttctct gcatagggtt tggtcgtaat actatcccct
     6241 ctatggaaat agtaataata gggtttagta ttaaccacat acttcttggc caacttgatt
     6301 aaatcaaaat ggtaataggc atcttcgtaa atcaacccct taggaaagga tagggcagtt
     6361 gcaatctgtc tcttgattag cttattgcaa atcgtcccag gtattttttc acctatgagg
     6421 tattccttta gaaatgtttg agaatcacag acaaaatagt catcctgatt ggctgactgt
     6481 gggctttcat cattagcata gacattcatg acaccacagc ttgaaacatc cgcatcttct
     6541 tgaactaatt gctcatataa gctctgaatc atttctggat ggatataatc atctgagtca
     6601 ataaaaatca gataatcccc gtgagcctgc ttcatcccat catttcgtgc ttgcgacaat
     6661 ccttcgttct ttttatgaag cactgacacc ctgtcatctt gttcagcgat tgaatcacac
     6721 aagcgaccac tttcatctgt tgcaccatca tcaacaagaa taatttccag attttgatag
     6781 gtctgcttct gaatggaagc tatcgatttt tctaggtact gcgccacatt atagactggc
     6841 acaatcacac taattaatgc agtttccatg ctactcctct aatagttttt ctacttgttc
     6901 gatttgtttt gtaattgtaa attgttgaat gaattggcta gcctcatcga catcaaagtt
     6961 tgaggcagaa gtcatgtaat tagtaatcgc ctgagctgcc tcttgattgc tctcaatgat
     7021 ttgtccaaat cgtccttctt gggataattc ctcagcccct ccaacgttcg tagagataaa
     7081 agggagtccc agactcaagg cctccacata cactccagga aaaccttctt gtttagacat
     7141 agacaaaaga actttcgtct gagatagata ctgataagga tttttttgat aaccaaggaa
     7201 atgtacatag tcctcaatcc catactcttt gactcgtttt ttcagttcct cttccatatc
     7261 accagccccg ataaaataga gatgatagtt ttttccctct tggtgtaata atcgtatcac
     7321 ttccactaca cggtcagaac ccttattttc ctcaatccgt ccgatagtac agatactttg
     7381 aggagcaatc tcgatatcga tcttctcttg agatttttct agaatagtct gaaaatcata
     7441 tccattgtag attgtctgta atttagaagt ataatctgga taaacttcct tgatagaatt
     7501 gctggtcttt tttgaaatcc ctacaattgt attcgcagca tccaactggc ttctatgtga
     7561 ttctctttta gagctatcct taagaagttc ttcaatactt ccatgaatcc aagatatctt
     7621 cttgacttct cttcttttag agaacaacag tggtggattc ataatggtaa aagaaacttc
     7681 aacatcataa tcatctttta caagcaaacg acgagccagt cttggaaaat aaattctcat
     7741 tctccacaaa aaagctcgta accatctggt ttggcgataa tcttgaaggg attttaaaat
     7801 gcgtacatgc tttggaacag attcatatcc cttgtcaaag tgctccattt caagaatatc
     7861 aatatcatac ttttctggat ccagatttga aacaatggtt gatagaatct tctctgcacc
     7921 acctccaaga gaaaaagacc acataaaaaa taagattttt ttcttagcca ccatattctc
     7981 ccttgtattc tgtataagac ttatccatat cagcgatgac agcatcatga tgcggtacct
     8041 gcttgtctgc tggtggaggc gtcatataat ccccaaaagc agttctgaga tagacatcat
     8101 agccgattgg aataggcatc tctgttcctt caaatggcaa gaaaagattg tcttcaaaag
     8161 atgtgattgg gtacttgttt ctcatgtagc caggacctga gcataattct gtaatgccat
     8221 cacaatcagc caaatcatac ttagtcattt ctttctcagc ttttttccag atgcgataac
     8281 agagagattt tggagtcaaa cccagtaaaa tgcgacttcc ccatttcatg agagcaccat
     8341 gcttttctgg aatagtttgc gcacaaaaga gtgaataaat caaggcccaa cgaacctgtt
     8401 ttttccgctc agctggattt ttcggataat aatccaaagg caaaacatcc aaggccagac
     8461 catgtggcaa atccaaatcc tgctgataag gcttgataca ggtggtttcc ttgtcacgaa
     8521 tggtaataaa aagattacga tcaacaaaat ccttgtgact ctttgacaag aaataacgtt
     8581 catctgcata acgaggccat aattctgcta atttctcata atctttacga ggcataaaaa
     8641 agtctaggtc gtcgtcccaa ggaataaatc ccttgtttcg aagggcacca atagcgcctc
     8701 cgccacagag ataacagagc aaatcatgtt ctttacaaaa ggccacaaaa tattcagcca
     8761 tctccagact acgagcctga attgctttta aatcagtcat attgttcatt attctttcta
     8821 tcgtatcgtt tcattatacc acaaacaagg ggtgaaaatc tattgcagac tgtaaaaaat
     8881 caaagcctga ctgctatcca aatagctatc aaactttgat ttttctgtct tatactcttc
     8941 gaaaatctct tcaaaccacg tcagcttcac cttgccgtag gtataggtaa ctgacttcgt
     9001 cagtcttatc tacaacctca aaactgtgtt tttagcagcc tgcggctagc ttcctagttt
     9061 gcactttgat tttcattgag tattatctta tctcaagccc atttgagcga gcttggtttg
     9121 atatttgttt tgatcaacca gcaagcccaa gcctccataa acatcatagg catctaccca
     9181 gtcacccagt tctggaatcg tcaatttttc aataccattt tttgctccat ccaaaacaga
     9241 taaaccgttt gttaggagga aagtataggg tacgttggtt gaggtcatag caaaaacctt
     9301 tccaagagct tcagaaccag tgaaaagttt agtgggatct ttaatttgct ctaaaattgc
     9361 tgttaaaact tgttgctgtc tttttgtacg gccgtaatcc gcctcatcat catcacggaa
     9421 acgagcataa ttgagcaggg gcgagccatt catctgctgt tttccgactt taatggtttg
     9481 ggttggagac tcagtctcgg tagcgtataa atcatctccg actgtagctt ctgttagggg
     9541 acgcccattc aatgttgaaa attgagcatc aatcgtcacc ccatcaggga aaagcgtgtc
     9601 aatcgctgtg gcaaaggcct ggaaatcaac caaggcgtag tacttaatgt ccaagtcaaa
     9661 attatctttc aagacttggc gaaccatttc tgcccctttt tgcccctctt gttctcctaa
     9721 ctcgtaggct acgtttaact tgttatctgt ctgttttcta ccgttaatca cttgactata
     9781 accatctata tagaccaaat tatcacgcat gaaactgact agcttcattt tcttatctga
     9841 gcccccgaca tttaatacca taatagagtc agttcgtgtc tcaacactgt tctggccgat
     9901 tcgaccatca gtacccatga ttaaaatatt aactccatct ctagtgtcct gaccattaaa
     9961 gacttctact tgagctgccc gggcatcagc agttttcttt gcgctagcat cttggtaacc
    10021 acgcaaaaac atgaatacca tggccaaagc cacacagacc aaaagtgaaa aaatcaccat
    10081 aaaaattcgt ttaagacgga gcttccgtct tttctttttt ggagggaaag agagtgcttg
    10141 tgatttggat tgtgagcgac tccggttcgc atagcttggt aagtcaacct gctcttctct
    10201 ttcttgttcc aagctagagc tactatttcc cctagcaaga gttagctttt cttgcaaata
    10261 ggcaaactca tttttttctc tctcattgag atagtgaata ttttttagca aataatcata
    10321 acgcaactgc tcatgatgac ttaagggatt ttctttactc atcttctctc ctttccatgg
    10381 tctgatattg gataaatagg ataggcaccc agaattttat actggattcc aatcgcttct
    10441 aattcttttt gggcaaagtg gaccaagtcc ttatcggtat aatccacatc gataatgaaa
    10501 aagtattcac ccagtgctgt cttgagtgga cgactttcaa tttttgtcaa gtcaattcct
    10561 cgccaagcaa aggtcgacag ggccttataa agtgcacctg gaaggttgtc aggtaatgtc
    10621 aaggccaaac tcatcttttc agtttgtgct tgcaagggaa tactaggctt ttcagctcct
    10681 agaacccaga aacgtgtgaa attggcttcc atttcctgaa tatcctcggc aatcagttcc
    10741 aatccatatt cttcagcaga acttctaggt gcaactgctg caaagggctg gtctggatgt
    10801 tcggaaataa aacgggccgc ataagctgta ctagctgtta cctcgatttg agcctctgga
    10861 tattgttcat cgatgaattt ctttccttga gccaaggcct gtggatgtga aaaaatcttt
    10921 tcaatcttag tatggcctgg aaccaccatc aactgctgat gaataggctg aacgatttct
    10981 gctactgctt ggatgtgagc ctgatgaaaa agatagtcca aggtttcatg aacactaccc
    11041 tcaatagaat tttcaactgg caccacagaa tagtccacta atccttgctc ataagccttg
    11101 atgacatctg taatgttagc aaaagcctgc aattcctcat gaggaaaagc tatctgcaca
    11161 acgtggtgtg aaaatgatcc cttgggacct agataagcaa ttttcatctt agttcctcta
    11221 taatttcctc tgggcttagc ttggtcacat ccaaaacccg actagccact tcctcatacc
    11281 aagcctgtct ttcttgaaaa atagctacta gttcttcctt gctattattt agaaaaagcg
    11341 gtcgctgatt gtccttatca gctgcgatac gttggtagag ggtttcaaaa tctgctttca
    11401 ggtagatgtt atctgtatta gtcttgagta agtcacgatt tctctgagaa ataaccactc
    11461 ctcctccagt tgacacgact tggtctgttt gtagtaaatc agctaggact tctgattcta
    11521 cctgacgaaa ggctgcttct cccttttcag cgaaaaaatt cgcaatggac atacctaggc
    11581 gcttctcaat cagagcatcc atatcaaggt aattagggtc caagcctctt gcaatagtcg
    11641 attttccagc ccccataaac cctaataaca ccttagccat gaatcaagct ctccaaatca
    11701 tcaaagaaac taggatagct ggtattgatg gcttctgcac ggtcaagctc cacctctcca
    11761 tctgcaacca agagggctgc gatagctgtc atcatgccga tacggtggtc accaaacgta
    11821 ttgactctag caccgtgaag agctgatttc cctttgataa tcatcccatc tgccgtagga
    11881 gtaatatctg ctcccatact atttaaggcg tctgccacaa cctgaatacg gtctgtttcc
    11941 ttgaccttga gctcctcagc atccttgata actgttacac cttgggcttg agtcgcaagc
    12001 agggcaataa tgggcaattc atcaatcaat cgtggaatca aagcgccacc aatctctgtt
    12061 cctttcaagt cagaagactc aacagtcaag gtagaagatt tagcgactgg atcgatttca
    12121 gttatttcca attttccacc catggcacga atgacatcaa taataccggt gcgagtttcg
    12181 ttgatcccca cattctgcag cactagacga gaatttggaa caatcaaacc tgcgactaac
    12241 caaaaggctg cactggaaat atctcctggt acgaccacct tctgtcctgt caatttttgt
    12301 ggcccttgga ctgtgatttt cttaccgtcc acacttaaat gaccaccaaa ttgtttcaac
    12361 atatcttcag tatgattacg ggtgcactct ttttcgataa taactgactc ccccttagct
    12421 tgtaaggctg caaacatcaa ggctgacttg acttgggcag aggcaattgg caactcataa
    12481 tgaataggtc ttaggttttt cgtccctttt aagcgaaggg gaggcaagtc tcgttcagtt
    12541 tgccctgaaa tgctgacgcc catttttttc agtggaaggg tcacacggtc cataggacgt
    12601 ttggaaagac tatcatctcc aaacatctct acttcgaaat ctgcaccagc aaggacacct
    12661 gaaatcaggc gaatcgaggt gccagaattt cccatattaa gggcattttg tggcgctttt
    12721 aagccagcca tgcctacacc ttgaatggta ataaccccat ctttatcctc aatttcaaca
    12781 ccaaggtcac gaaaaacctg catggtcgaa agaacgtctt cacctcgcag aatatcataa
    12841 accttggtct caccctcagc caaacttcca aagataatgg aacggtggct gatagacttg
    12901 tcacctggga cgcggatact accatgtaaa tggcgaatgt ttgtttttag tttcatactg
    12961 gacctcatac ttgcaatact tttacctatt ttatcataaa aagccagaaa ttccttaaaa
    13021 attcctgact ttaggatcgt tcttttctta tttcagcaat tctgaaactg gttcaaaaac
    13081 aattttttca atgtcagaaa ggtaaatggc caattgttgt tgcttggtaa agaattctga
    13141 caagaggcta tttccttgaa tctgtttacc aaagccttcc atcttagctt ggaaggacgc
    13201 atctggcatt tgacctgtat gtgctagttt ttgaatttcc tcttgaaagg caagatattc
    13261 tgtaaagatt ttgcttgcct cagcatctgc tgcaatcgca tctttagctg ctttaacagc
    13321 cttgtattct ggtaatccgc gtagaccgcg actgagttcg tttgcactat cgtaaatatt
    13381 tgacatgttc ttctccttat ttgatgacga ctgtatagtc ggtattttct gttatgagat
    13441 gctcagctct ttccaagtct tgagcatttt taaatgaaat ttgtaggatt ccgtgaatat
    13501 cctcacgatt ttcctcgttg atgtggatgt taaccaagga agtcccacgt agcagttcca
    13561 aaatccgcag gatgacatct tcttcatcag gaacgtcaac atagaggtcg taagagctat
    13621 ccacaccacc acgcttatgg atttccatgg tctggcgttg ttcacgcgct tggttaaaaa
    13681 agttccaaat ttgctcttca tctcccttac taatggcctg accaatcact tccaaacgtt
    13741 ccttgaaatc ctcaattcta tccagaatgg tctcgctatt ggacaagaga atggaggtcc
    13801 acattcctgg ctcgctttcc gcaattcggg tcatatctcg aaaaccacct gccgcaaagc
    13861 gccttgccat ctcatgctct tgagcataga ccgcagtctg ctccatgaga ctagaagcca
    13921 aaatatgagg aaaatggcta atctgagaag tgacacgatc atgctccttg gcatcaatct
    13981 cgataaaacg agcatgaaga cctgaaagca gatccttcat ttccttaagc gtgtcctgac
    14041 ttgtcaggct tgaaggtgta aagatataat aggcattttc aaaaagattg acatctgccg
    14101 aagcagcccc tgtcttgtga ctaccagcca tgggatgggc cccgacaaag cgaacagact
    14161 tgccagccaa atactgctcc gccgcatcca caatggttga cttggtcgaa ccagcatctg
    14221 aaataataac gccttctcgc aaatccaaat tggccaactc cttaatgaaa gcaatagttt
    14281 gtttgattgg caagctgagg ataatgacat ctgccaaagg agcaaaacta gcaaaatcat
    14341 ccgttgcacg gtcaatcatg ccttctttca aggcgatatc tctcgaagct tgactacgat
    14401 tataacctaa aatttcataa tctggatgat cgcgtttgat accaagtgcc atagaggctc
    14461 caatcaaccc aagacctgcg atatagattg tttttgccat aggaactcct taatagttct
    14521 ttgtatagtc tcggtgtttg gctaccgctt cttttagttc ctcaagatta tctgatgaga
    14581 atttttcgag gatttcttgc gccagaaccg ttgctacaac tgcttccatg accattcctg
    14641 cagctggaag agcagtcgga tcacttctct ccacggttgc cttgtaaggt tcgtgggttt
    14701 cgatatccac actcataaga ggtttataaa gagtaggaat gggtttcatg accccacgaa
    14761 caacgatggg ttgcccatta gtcataccac cttcaaaacc acctagatta ttggtacggc
    14821 gagtataacc gtcttcttta gaccagagaa tttcatccat aacttggctg cctttacgat
    14881 aaccagcctc aaagccaaga ccaaattcca cccctttaaa ggcattgata gagacaacag
    14941 cttgagccaa tcttgcatcc aattttctat cccattggac ataggaacca agaccaactg
    15001 gaacgcctcc gacgactgtc tccacaaccc caccgatggt atcaccatca cgtttgattt
    15061 ggtcaatata gtccttgatt tcctgttctc gttcttggtt aacaatagaa acttcagact
    15121 gggcagctct ttgcttaatt tcagcgactg tcagattttc aggaacatcg atttccttgc
    15181 caccaaagac cacgacatgg ttggcaatct ccatatccag ctcagccaag aggcgtttgg
    15241 ctactgcacc aactgccacc cgcatggtgg tttcacgagc tgatgaacgc tccaaagaat
    15301 ttcgcaaatc atcaaaacgg tacttaatcc ccccaaccaa atcggcatga cctgggcgag
    15361 gatgagtaat tttccgcttg cttttaaggc ggtcttcaat gtcctccgca gacatgatgt
    15421 ccagccattt ctggtggtcc ttattgatga catccatagt aataggcgcc cctgtcgtct
    15481 tcccgtggcg aacgcccgaa gtaaagacaa cctggtcact ctcaatcttc atacgaccac
    15541 cacgaccgta gccaccctga cggcgtctaa ggtcctcatt gatatcctca gctgtcaatg
    15601 gaagtccagc tggaattccc tcaataatag ctgttagacg ggggccgtgt gattctcctg
    15661 cagttaaata tctcatacac tctccttatt ttaccaagta gtctttcatc tcttccagag
    15721 aaactgggtg aatgatcgct gaaccaagct ctgacaccaa gaccaatttc aaggtgttac
    15781 cacgcgtttt cttgtcatga gtaagagcct gataaagctt gtcaacttcc caattttcat
    15841 agtcaacagg caagccgaat ttctgacaca tctctgtgat agattgggta atgccagctg
    15901 gcatgaggcc tttttcctca gcaaccttgg aaatctgtac cattcccatg gcaacagcct
    15961 ctccatgcat gaccttgcca taaccggcag tcgcttcgat ggcatggcca atagtgtggc
    16021 caaaattgag gtaaagacga ataccattgt ccaactcatc ttcaaccacc atcttgcgct
    16081 tcacctgaca agaatgttca atcaaggtct ctgcatgttc caaaatgctc tcaacagaac
    16141 cattcagttc cgtcaagaga gcccacagtt ctggatcctc aatcaagcca tacttgataa
    16201 cttcacccat cccttcaatc aactctcttt ttccgagggt ttcaagaaca agtggatcaa
    16261 tcagaacccc atctggttgg gcaaaggtcc ccaccatatt tttagcaaat ggtgtgttaa
    16321 cgcctgtctt tccaccgata gaagaatcaa cctgagctgt caaactagtc ggaatctgaa
    16381 caaagtgaat accccgcata taggtagagg ctacaaatcc agccaggtcc ccaacaacgc
    16441 caccaccaag agcaacgatt ccatcgctac gagtcagacc ttgcttgact agaaattcat
    16501 agactttctg aacagtagtt aaattctttc tttcttcacc ttctaagaaa tcaaaaacag
    16561 ctacctgaaa accagcatct tctaggctga gcttgacctt ctctgcatag agagaggcta
    16621 catggttatc tgtcacaatg actacctttt gcggttgcca gagttctcgc aaccactgac
    16681 cagcctgggc catacaacct ttttcaatct gaatatcata aggatgatga ggaatatcga
    16741 ttctgatttt cataggagag tctccctttc tttattggta tttttctgtt aaagactgcc
    16801 aaatctcttc tgtcggcatt tccttgcctg tccacagttg aaaagcttct gcagcttgat
    16861 agagcaacat tcccagacca ttgactgctg gattgccctg acttctagcc catttcaaaa
    16921 acggtgtttc aaagggttgg tatatgatat ctgcaactaa gagagtttct ggtaagacta
    16981 tgttttcagg aacaggagag gattggccat ccatgcccac actggtggca ttaactagca
    17041 aatccgactc ggcaatcctt gcttgcagtt cagaaacatt ctctaaagca cacaaatcca
    17101 ctttaaaacc tgtctgctcc tgtaacttgt ctaggtaagg tcttgttttt tccatagaaa
    17161 cggaacgaac aaagaccgaa atctgactga cgccatccaa aatagcctgt gccaagattg
    17221 atttagccgc accacctgca cccagcaggg tcatcttttt acctgaaatt gtaaaagaag
    17281 gcaagcactt aaaaaatccc ttgccatctg tattatatcc aattaaattg ccattctcat
    17341 tgacaaccgt attaaccgca ccaatcaagc gcgcttcatc gctcagctta tccaaataag
    17401 gaatcacctg ctccttatag ggcatggaca gattgatgcc aaacatctgg tagcgacgaa
    17461 tattggccac tgtttctgcc aagtcactcg cttcaatctc ccaagccaca taagcaccgt
    17521 tggtagctgt cgcctcaaag gctctattgt ggatgaaggg agaaatagaa tgcttaatag
    17581 gattggcaac aactgcagct aaacgtgtat agccatcaag cttcatccaa aatctccctg
    17641 atttttttca tgctagctag agaaatctgc ccaggggcac taacctcatc cagactggca
    17701 aaagaccaac tcgaaccagt cacatccgca gtgatacgag agaccttgcc caccttaccc
    17761 atagaaatgg tcacatattc ctgttcagga ttgagggttt taaagcctcg tgtatagttc
    17821 atcaagtcta agacatcctg ctccgtgtga gccatcaccg caaccttaac aagttttgga
    17881 tttaggatcg tcaactctga caagatttcc atcatgttct caggtgtttc ttggaaatta
    17941 tggtaactca aaacaagatt tgggaagtcc agcatttcct caaaaacatc cttgtagcta
    18001 tagtactcaa aatcaatata gtctggttga tagaattgcg caacttcctt gattagatgg
    18061 atatactctt ctggagaaag gtcgatttct ccaccttcgg agcgagttcg tagcgtgaaa
    18121 accaactcac ggcctgcgaa tttttcaaaa atggctggag ctacctgcaa aatcgcttct
    18181 ttaggcagat agtcggcacg ccattcaatg atgtcggcat ccaggtacct cgtggcatcc
    18241 agagcctgag cctcctctaa acttcttggc attactgaaa cgattaattt catttactaa
    18301 ccttcatact aatcaccttg aggtaattac tactttcatc ttttttatta taggcaaaat
    18361 ctgctggaag accatatttg tttaaaatct ggtaacttct tcctgcaaag cctttatcaa
    18421 tttgttctgt aaatttctga cgggaaacat tggcagcatt ggtactggca atgataatcc
    18481 ctcccggatt taaaatctca agactctggg aaatcaactt gtgataatcc ttggccacag
    18541 agaaagtttg ttttttattc cgagcaaagc taggcggatc taggacaatc acatcgtagg
    18601 tcaagtcttt gcgtttggca tatttgaaat actcaaagac atccatgact ataaaacgat
    18661 gctcgtctgt gctgagccca tttgcctgaa aatgcgcttg agacaattct cgtgaacgtt
    18721 tggctagatc aacagaagtt gtctggctag ctcctcccat ggccgcagct actgaaaaag
    18781 ccgctgtgta ggaaaacata ttgagcaagg atttacccat agccaagccg tcaactaaac
    18841 taccgcgaac ctcatgctgg tctaggaaaa ttcctgtcat caagccatca ttcataaaga
    18901 cttgatacag gacaccattt tctaaaacag tgaaaaagtc aggtgcttct tgaccataaa
    18961 catgggcaga ttcatagtca aaacccttaa agcggatttt ctcataagcc cctaaaacct
    19021 cagggaaaac ctgtctaaag gcttctgata tagtctgacg aatctgataa acataagagt
    19081 tataccaaga aaagacggcg tagtcgccat aaaggtccac tgtcagaccc ccaaagccat
    19141 ctccctcttg attaaagaga cgaaaggcag ttgtcaaatc atcttgatag taggcgtttc
    19201 tcttttcttt ggcttttcta aacaacgttt caaagaaagt ttgattgaag gccaccttgt
    19261 ctttgctgac aaaccagccc aagcccttgt tttgctgaga aaggtaggca gtcccaagaa
    19321 agtttccttc ctgaccctgc acctctactt cctgatcctt aagattgaca ttctcaagat
    19381 cactggcttc tagtaaaact agccccttag caagcttctt ttcaaccctt ttgctgactc
    19441 ttattctatt cataactacc attatatcaa acttttagac aattctcaaa aaagaaacta
    19501 cccttgcttt tttactcttc ttttaaaaaa tggtatacta gacttcctgc aaaactagga
    19561 aataaatgtg taagaatcac aataaaaaat gctcttccgt cttggaggag catttctttt
    19621 tatcaacgaa aatcaaatag caaactatga aactagcctc aggttaaccg tgagattata
    19681 ggtagagagg ttgtatcagc aatatgtgtc tgtcaaattt agtgacaaag gtagtagaag
    19741 aaagatgaag aaataaatca gcttcagtag gtatctggaa aatttgattt tatagagaag
    19801 ccttttgtta caaactcaat atactatcaa taaataatat tatagaagca acaataatta
    19861 aaatttcacc tatttgcatc attctatttc gaactctaaa tatatgttct atcaaaaata
    19921 cttggatcac acacattata ggaattaacg tttttgaaat tgaaaaatat cccaataaat
    19981 aaactataaa caacaataat agaactatat tatatttctt attcaaaaca ttcctcccta
    20041 tatttttgat taccaatctt aatcatttac aactacattc taacaaacta taaaagcgtt
    20101 tgtcgaattg aatttatcaa gcaagcaacc aactatctca tcttttttct atttctgcca
    20161 atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcaag acgataagag
    20221 ctcctactcc caagttgatg gcaaggatag gggagagaga ctgaaccaag aatatgctcc
    20281 caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg gcgactatgc
    20341 catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga tttttaacgt
    20401 ccttaaagta atggtggcaa gaaaggatga cactggcaat gatccagact acaagaaggt
    20461 aaatcatcga aaggatggac aagcctagat atagagaaag accgagcaaa gtcagaactg
    20521 gtaaaaagga ctgaacagca aagataatcc aaaatttcac tttcacataa cgagcaaagt
    20581 caaagggtaa actcttcaga aaatcaacat tttccctctc caaggacaag gcaattgaat
    20641 gcaggctggt gatattgtta ttgacaactg ctataaacac tcctacaaaa aacaagggta
    20701 accagtatgg aggatgaatg tctggaacta tctgagaatc tcggattttg gaaatcagac
    20761 tgatcatcat gagataagga aggaaagcac ttgtaaaaag cactgtaatc acaccagtcc
    20821 cctgtcccaa gagggtgagg tggtagcgta aaaccatacg gaaaaagccc tttttagtag
    20881 ttgaaatcct ctccttgctg cgacgttctt ttttgacctt ctcctcactg ttaagcagga
    20941 tcacgtcata aaaacgagga aggaccttct ttttggtcag ataaagcagg aagagagtta
    21001 gtcctatcca agcgagcaga cccactaagg cttctgtcga aaaaggctcc actgctattt
    21061 tgtaaaagat atgaagagga taaaggagaa atggaatgtc tctaactttg tcaacaatac
    21121 ttccaaaagt cgactgaaga aagaagataa atattaaagg tatgagaact cctatcccaa
    21181 tcatcacatt cgaaaaaata gactgatact ttctgaagac cctagtctga gccaagaaat
    21241 gtactgccac taccgtcact aaagtaacag agacaaataa taaggtcaag gacagtagca
    21301 tcaaaggcaa acccagccaa agagaaggag ctagactaat atagagggct agaaaataag
    21361 ctaggattgg tacaattcca gttagagctg gcaagaggac agacagacct ttggccatta
    21421 taatctctga ttctttaaag gcatagggcc tataggaggc caggtcctta ctctcataaa
    21481 agacattgta aaaggcagtg aaagaagttg aaaaggccag caccagtaaa atagcaacca
    21541 tcgagctaaa ataaataggc atttcctcaa aaggaaaatg aatggctata ttactaaaac
    21601 agatgagcat caagagactg gaaaaaatgt aagaacttag gactctagca gaaacattta
    21661 cttttttccc aggattctta gcctgcttct ttcgtagatt agccagatta gcttcttgag
    21721 atgaatagag gatattgata tcaactaatt ttttaatgac cttgagacgc atccgcaacc
    21781 tcctcttttc taccagcaag actaaggtag atactttcca aaaactggtc tgggtggtct
    21841 ttcctcaagt cctctacctt accacaataa atcaaatgcc cctttttcaa aatggcaatc
    21901 cgatcacaga cttgctctgc cacctctagg acatgagttg aaaacaagac tgtcttccct
    21961 ttttgtgcat gttccttcat catctgtttc aaatcaaagg cagcctgggg atccaaacca
    22021 gtcaagggtt catccaagac ccaaatatcg ggatcagaca agagtgctcc gatgacaaag
    22081 actttctgac gcattccgtg agaaagagtt tcaataacct gatagcgatt ttcagcaaaa
    22141 tcaaaaacgt tcaatagcct agctagacta gcctccaagt cagatctact cagatcatag
    22201 gatgaggcga tcaattccca aaattcattg gccgttaagc gtaaaaataa gtcaggcgag
    22261 tctgctacgt agccaatctt tcgtttaata gccaagcgat tttccgataa ctcctgaccg
    22321 tctaccaaaa tacgaccact gctgggtgaa atgatactga ctagggattt tatggtggtc
    22381 gattttccag ccccattatg accaatcaag cccataatct ctccattttc aatctgcaaa
    22441 ttgagattac tcaaggcctc tttatcacca tacaacttac taacattttg aaattcaatc
    22501 atgggacaac tcctatcttt atctgtatta tacatcgcta tactagcact ttgatacaaa
    22561 aaaatcaata ctttatttta agtagttaaa atagtttcct tctatcttac gcaaacgttt
    22621 gcaccaagag atactttatg atagaataaa gaacaagatt gacaagtaag aggaaacatc
    22681 atgcaaaatc aaacactcat gcaatacttt gaatggtatc tgccccacga cggtcaacac
    22741 tggacgcgtc tggctgaaaa tgctccacac ctagctcatc tggggatcag tcacgtctgg
    22801 atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt ctatgactta
    22861 tttgacttag gagagttcaa ccaaaaaggg actgtccgca ccaagtatgg tttcaaagaa
    22921 gactatcttc aagccattca agcccttaaa gcacagggaa ttcaacctat ggccgatgta
    22981 gttctcaacc acaaggctgc tgccgatcac agggaagcct ttcaggtcat cgaagttgat
    23041 cctgtagacc gtacagttga acttggagaa cccttcacca tcaatggctg gactagtttt
    23101 accttcgatg gtcgccaaga tacctataat ggcttccact ggcattggta ccacttcacc
    23161 ggtacagact acgatgccaa acgcagtaaa tctgggattt atctgatcca aggggacaac
    23221 aagggctggg ctaacgagga attggtcgat aacgaaaacg gaaactacga ctacctcatg
    23281 tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga ctgggctgat
    23341 tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa gcatattgac
    23401 tctttcttta tgcgcaactt catccgcgat atgaaggaaa aatacggtga cgatttctat
    23461 gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct cgaaaaaacg
    23521 gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga agccagtcaa
    23581 gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga actcaagcct
    23641 gacaaggctg tgacttttgt cgacaaccac gatacccaac gaggacaagc ccttgagtct
    23701 accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg ccaagacggc
    23761 cttccatgtg tcttttacgg agactactat gggatttcag ggcagtatgc tcaacaagat
    23821 ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag atttggccta tggagaacaa
    23881 aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc tgaaaatcaa
    23941 tccccaatcg cagtccttat ctcaaatgac caagaaaaca gcaagtcaat gtttgtcggt
    24001 caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg tcaagttaca
    24061 attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag tgtctgggca
    24121 gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt tggctttttt
    24181 gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa tttacctgct
    24241 actgcatcca acaattcttg gatcttagct tggttgcttc ctcctgccat ggccatgtct
    24301 ggtttaccac caccacgtcc atcgatgatt ggtgctaatt ctttgacaag gtttcctgca
    24361 tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat agcagcaact
    24421 aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaggt acgaagggca
    24481 ccggcatctg atacagacac ttgactagca atgtagcggt gaccgttgac ttccttcaca
    24541 tccttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc agcattttct
    24601 ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac ttccttgact
    24661 tgaggtgctt tcaaggttgc tgcaacagct ttaagagcgt cctcttgttc acgataggct
    24721 tcaaaggctt ccttaccagt cactgccaag atacgacgag ttcctgaacc gattccttct
    24781 tctttgacaa ttttgaagag accaatctca gaagtgttgc caacgtgggt accaccacaa
    24841 agttcaatag agtagtcacc gatagtcacg acacgaactt ccttgccgta tttctcacca
    24901 aagagggcca tcgctcccat ttctttagca gtgtcaatat ccgtttcaac tgtcttaact
    24961 tcaagagctt cccagatttt ttcgttgact tgctgttcaa tcgcacgaag ttcctcagca
    25021 gttactgctt ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt aagagatcct
    25081 gcctgtgttg cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa atgagtcgca
    25141 gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt atattcttgg
    25201 ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg cttgaccatt tggggctttc
    25261 tgaacattgg tcacagtagc cacaacctta cctgactcat ccaagatttg tccgtagtca
    25321 gctacctgtc cacccatttc agcataaaat gacgtttccg caaagataag agaggcagtt
    25381 ccttctgaaa cagcttctac ttctgcattg tccgccacga tagctaccaa tttagaagac
    25441 aattggctag cattgtagtt gaagacactt tctacagtga tgttttgaag agtttcattt
    25501 tgcataccca ttgagccacc cttgacagct gaggcacgcg cgcgttcttg ctgttctttc
    25561 atggctgctt caaaaccttc acggtctaca gtcataccag cttcttcagc gatttcttca
    25621 gtcaattcaa ctgggaaccc ataagtatca tagagtttga agacatctga accagcgata
    25681 acagattgac ctttttcttt caagtctgct acaatgcctt gggcaaagtg ttgacctgag
    25741 tgaagggtac gggcaaatga ttcttcttcg ctcttaacga ttttctcaat aaagtcacgt
    25801 ttctcaagca cttctgggta gtagctttcc atgatttttc caacagttgg aaccaatttg
    25861 taaaggaaag gctcgttgat acccaatttt tgaccatgca tagaagcacg acggagaaga
    25921 cgacgaagaa cataaccacg accttcattt cctggaaggg caccatcacc gatagcaaat
    25981 gaaagagaac gaatgtggtc tgcgataacc ttgaagctca tgttgtcgcc atcttggtca
    26041 taaaccttac cagacaattt ctcgacttca cggataatcg gcatgaagag gtccgtttca
    26101 aagttggtct tagccccttg gataacggcc accaaacgct ccaaaccagc gcccgtatca
    26161 atgttcttat gtggcaattc cttgtattcg ctacgaggaa cagcagggtc tgcgttaaat
    26221 tgtgacaaaa cgatgttcca gatttcaata taacggtcgt tttcaatatc ttctgcaagc
    26281 aggcgaagac cgatattttc tgggtcaaag gcttccccac ggtcaaagaa gatttctgta
    26341 tctggtccag aaggtcccgc accgatttcc cagaagttgt cctcaattgg aatcaagtga
    26401 cttggatcca ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc tggatagtag
    26461 gtcatgtaaa gtttttcagc agggaaatca aaccattcag ggcttgtcaa aagctcataa
    26521 gcccaagtga tagcttcgtc acggaagtaa tccccgatag agaagttccc cagcatttca
    26581 aacatggtat ggtgacgcgc ggtcttccct acgttttcga tgtcgttggt acggatagcc
    26641 ttttgggcat tggtaataca tggattttca gggataatgg tcccgtcaaa gtatttctta
    26701 agggttgcta ccccagagtt gatccacaaa agagttgggt catttacagg aaccaaactt
    26761 actgatggtt ctactgagtg acctttggtc gcccagaaat caagccacat ttggcgtact
    26821 tgtgcactag atagttgttt catattgtct ccttattcac ttgtttaatg tgattggctt
    26881 tccagcattt ccacatagtc aatcgcgaca cagagggaaa tgactaggtc tgcataagcg
    26941 tcttcaagaa ccgttacggt ataggtagag gtcagatgga agagttcctt cttaatttcc
    27001 gcaatcaact gatcgcgatc atccagcaat ttgaaattca aatcccagat attgccctcg
    27061 atacgaagac ctagattatc aaactcatac ttatctcgcc agaaggtcaa cttcttacga
    27121 atgacaaaac tcgagccatc ccgaagctga atctcaaaac gaggaagcaa ggtcaagatt
    27181 tctttactaa tctcactgac ttgttcacca gccgcatcat agatggtaaa ggttttagga
    27241 atcttaaaaa atgatccctc cacctgatag gcaatttctc ccctgtcatt cttgatagcg
    27301 aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa atgttttcat caacacctcc
    27361 aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc accttcattc
    27421 aatgaacttg tcattctctt gttcttatgc aattgtatga ttgagtagca tgacttccta
    27481 gcttagatgg ctcgcagcac cgccatttct ctggactaag acaagtgaaa atcaattctc
    27541 aactttctta ttatatcgtt tttttaatct tgcgtcaact ggaaatgatc tccgttgaat
    27601 tagaccaatt ccctacatct ctgattactt tttcaggata tattttttct tactgccatt
    27661 tttcttttta tcccaaattt tcatattact aaacacagct actagaatat ttccaaatat
    27721 aaaggtgcct atcacccaat atatggactc agttgttagg tattgtcgat ccaagccatc
    27781 ctttaaatgg aatagtataa cagtttggtt aacaatcata aaggttggcc agaaactttt
    27841 tttgaaaaaa gtagacattt tcattatttg ttgccgcttt ctgtaaggtt aatactcaat
    27901 aaaaatcaaa aagcaaacta ggaagctagc ctcaagctgt acttgagtac ggcaaggcaa
    27961 cgctgacgtg gtttgaagag tataggctta gtatactact aggcaagcaa ataagcaaat
    28021 aaacaaataa acaactagaa tagaaaaaga tagggctcta aaaactgact tctattcctt
    28081 aaaaacgaac cagcttgact gattcgtctt cttacgttta tctcctactt ccgatacatt
    28141 ttaaactgta ggaagaggtc gctatatttc cctgtccatt tatggtcaaa tttctcataa
    28201 acttctaggt gtttcatggt ttcaacatcg ggatagaagg ccttatcttc ctttgtttcc
    28261 tctgggagca attccttcgc tggtaggttt ggtgttgaat agccgacata ctccgcattt
    28321 tggagagcat tttcaggttt caacataaag ttgataaagg cataggctga gttttggttt
    28381 ttaactgttt tgggaatgac catattgtca aaccaaagat tgctggcctc tgtcggtacc
    28441 acataacgta gattttcatt tttttctaac atttggctgg cttcaccaga gaaggtcacg
    28501 ccgattgcaa cattattctg aatcatatag cccttcatct cgtccgcaac gatagccttg
    28561 atatttggag tcagtttgta gagcttatcc actgtctctt ccaactgctg cagatccttg
    28621 gagttgaggc tgtagccgag ggaattgagt cctagtccca gcacctcacg cgccccatca
    28681 aagagcatga tagaattctt atactccggc ttccaaaggt catcccaatg ctcaggcgct
    28741 tcatctacca tggtttcgtt gtagacaatt cctaaggttc cccagaagta agggatggag
    28801 aatttattac ctgggtcaaa ggactggttg agaaactctg gtccgatatt ttcgattcct
    28861 ttaatttttg aataatcaag cggaaccaag aggtcttcgt ccttcatctt gttaatcatg
    28921 tattcacttg gaatggcaat atcgtaggtc gttccaccct gctttatctt agtgtacatg
    28981 gcttcgttgg agtcaaaagt ctcgtactga acttgaattc ctgtttcttc tgtaaactga
    29041 gtcaagagtt caggatcgat atagtctccc cagttataga taaccaattt ttgactatct
    29101 cgactattga ttttactatc taaatgagtc gcaattcccc acaagacaag gataatcgct
    29161 gcaattcctg ctaaaaatga atagattttt ttcatgcttg ctcctccttc tcacgagaga
    29221 taaagtaata acctacaact aggataatac taaagagaaa gactagagca gacagggcat
    29281 tgatttctaa ggaaatcccc ttgcgagcac gagagtaaat ctcgactgat agggttgaaa
    29341 agccatttcc tgttacaaag aaggtcacgg caaaatcatc cagcgaatag gtgaaggcca
    29401 tgaagtaacc tgcaatgata gacggagtca ggtaaggaag catgatttcc ttaaacatct
    29461 gaaattgact agctcccaag tcataggccg catgaatcat gtcgccattc atttccttga
    29521 gtcgaggcaa gaccatcaag accacgatag gaatggagaa ggccacgtga ctagatagaa
    29581 cggtcaaaaa gccaagtgaa aacttgagtt gggtaaagag aatcaagaag ctagcaccaa
    29641 tcataacgtc aggcgcaacc atgaggatat tattgagtga tagaaaggct tcttggtatt
    29701 tcttacgaga ctggtagatg taaatggcac caaaagtccc gataatggtc gctatcaagg
    29761 ctgataggaa ggccaagaaa aatgtctgag ccaaaatcag catgagtctc ccatctccaa
    29821 acatggtttc aaagtgagtc cagctaaaac ctgtaaagct attcatatca tcaccagcat
    29881 taaaggcata gccaatcaag taaaaaatcg gcaggtagag gaccagaaag accagtccca
    29941 gataaaggtt ggcaaatttt ttcatcgttc tctcctttcc ttggtcaccc acatggtgat
    30001 gaacatggtc aggatgagaa tcacaccgat ggttgaaccc ataccatagt tgtcattggt
    30061 tagaaaattc tgctcaatag ccgtccccaa ggtgataacg cggttcccac caatcaaacg
    30121 ggtcagcatg aagagactca aacttgggat aaagaccgac tgaaccccac ttcgcacacc
    30181 gttcatagat agagggaaga tgacatgacg gaaggtctcc cacttagttg caccaaggtc
    30241 gtaactagca ttgatgagat tattatccat atcgtctaag acattgaaaa tcggcaaaat
    30301 cataaagggg agctcgatgt agcttgcgac aaagataaag gaaaaatcgg taaaaagcaa
    30361 ctgttgtgaa ccaattccga taaattccaa gaattggtta atagagccat tttgaccaaa
    30421 aatcccgata aaagcatagg ctttaaggag caaattgatc caggtaggaa ggataatcag
    30481 catgagccag agttgacggt gcttgagacg ggtcaaaaag agggctgttg gatagctgat
    30541 aagcaaggtc acaaaggtca caatccctgc ataaagcact gagttgaaac tcattttgag
    30601 gtaggtcaag ttttgtgacg caaagtaaga tttataattt tctaaactaa actggccttc
    30661 aatgttgaaa aaggattgtc cgaaaatcaa gaccaagggt gccaatacaa agagggcaat
    30721 ccaaagcatg tagggcacta caaagagttt agagcttgtt ttcttcatct ctttcctcct
    30781 cgatcgcatt gatcagacct gcttcttgct cttcgatttc cacgtattct tcgatacgag
    30841 catcgaactc ttcttcggtt tcattgagac gcatgatgtg gatgtcttct gattcaaagt
    30901 ccagaccgat ttcctcaccc acgatggcct tacgggttga gtggatcatc cattcatttc
    30961 caagttcgtc ataggcgata atttcataat gaactccacg gaagagctgg gtgtcgacct
    31021 taacttggag tttgccttct tcaggaaggg taatgcgcaa gtcctctgga cgaataacga
    31081 cctcaacagg ttcatttggc ttcatcccac catcaaccgc ttcaaagcgt ttgccgttaa
    31141 attcgaccaa gtagtcctca atcatggtac ctggcaagat gtttgactcc ccgataaagg
    31201 tggcaacaaa gtggttgatt ggctcatcgt agatgtccac aggggttcca gactggacaa
    31261 tctcgccatc attcataacg aaaatccagt cactcatggc aagagcttct tcctgatcgt
    31321 gagtgacaaa gacaaaggta atgcccaatc gttgttgtaa ttcacgcaat tcgtactgca
    31381 tgtctgttct caatttcaag tccagcgctg ataaaggctc gtccaacaag accacacggg
    31441 gttggttgat gatagcacgg gcgatggcca cacgctgacg ttgtcctcca gaaagtttgc
    31501 ggatggaacg tttttcataa ccttccaact gaaccatctt gagaacttcc gctacacgct
    31561 gctcgatttc tttcttatca attttacgca agcgaagtgg aaaggcaaca ttttcaaaca
    31621 cattcatatg tgggaacaag gcataggatt ggaagacggt atgtacgtcg cgcttgttgg
    31681 ttggaatatc attgatacga acaccgtcta gcatgatatc tcctgtcgtc gcatccagta
    31741 aacctgcaat aatgtttagg atagttgatt tccccgaacc agatgcacct agaagggtgt
    31801 agaatttccc ttcttccaac tcaaagttga tgtctttgag aaccttggtg ttgctgtctt
    31861 caaaaacttt agagacgttt ttgaattcga taattggttt tttcaattgg cataaattcc
    31921 ttctttttca tagattaacc gatcggggct ctgtcaggtc cccactacct cttgcaggga
    31981 gtaaaaccac ctgcatacat cttcgctacc gataggcttt cacccccttt ccatgacata
    32041 gcagcagctt ttcaaataca gcttcctact tgctttcacc caagatccgg acttctcttt
    32101 caagcgtaat acctgagtgt tccttgactt tttcgataac cgattggatc aagtcctcgt
    32161 agtctttggc cgttccatct gcgacattga tcataaatcc tgcatgcttt tctgacactt
    32221 ctacgccacc gatacgatag cctttcaagc cagcttctga aattaactga cctgcaaaat
    32281 gcccgactgg acgcttaaag accgagccac aagatgggta ttccaaaggt tgcttgagtt
    32341 cacgtaggtg cgtcaagcgg tccatttcct gcttgataac ctgatgggtt cctggggcta
    32401 gggcaaattt aactgacaag acaactgcac cagactcctg aatagctgaa tggcggtaac
    32461 caaaagccaa gtctttagca gacagggttt cgatttctcc atccttggtc aagaccttac
    32521 aagactgcaa gatgtgagca atctcgccac cataggcacc cgcattcata aagacagcac
    32581 cgccaacgct tcctggaata ccacaagcaa actcaaagcc agtcaaacta tgacggaggg
    32641 caatgcgagt tgtttcaatc aagttagccc cagcttctgc ttcaatggta tagccatcaa
    32701 cagaaacgtt attgagcttg tcacacaaga tgacaaatcc acgaatccca ccatcacgaa
    32761 cgatgatatt gcttgcattg ccaagaacca tccaagggat attttcttgg ttggcaaatt
    32821 tcacaaggcg cgccaactca aaacgatttc gtggaaagac caaataatca gcctctccac
    32881 ctacttttgt ataactatag ctatgcaagg gttccttaaa acggatatca attccttcta
    32941 agatttcaag cattttttct cttacagaca tgtcactctt ccttttacaa aattcattcc
    33001 attataccat ttttagagac atttgacgac cataaaaata ccttgtttgg attttgcata
    33061 agaaaaagag gttcccccct ttttatgatt ttttacaaaa gatttccttg gttccatagg
    33121 cgaccagaac gagctccagt gctagaatca cttcaaccaa gactggattt gtcaaccagc
    33181 ctacttggaa aagagatggt gccagatcaa agaaggcatg caagccatag gctgctagga
    33241 gataaatcca tttcttctgg cgaacagctt ggtaaaccca aactgtcaaa agtaattgga
    33301 aaccaagcgc caagattcgc tcaaaaccaa gcaaataaat ctgccagacc gaaagtgact
    33361 gaatggtttt taacatattt tcagacagta attgcataac ctgtggattc tgagtttgaa
    33421 ctgccgaaag aacgatgtaa agattgagta aactaataag gcctagaaaa atcaactcca
    33481 agccaccatg ccctaggcca taggccaagg catctgcctt ttccaaactt ctctttttct
    33541 ccaaccattt gaagaaaaca agacgagccg tttcctcaaa aaatgctgcc atggctaggc
    33601 catagataat atagataagc ggatgatctt gcaagagggc aatactaccg tctttttgag
    33661 ggtgcaaaat caagatatgc accagttttt ccaaaatctg cgaagagaca aagaaagcga
    33721 ctgcacccaa gcccaagaca gctagattaa tctggtattt ctttttggca aaccaaatgc
    33781 ttcctatcag aaaagctagc aacagcaaca tggtaataat gatatgaatg gtcattttct
    33841 tctcctattc tgccttttca atatcttttt tcatctcgtc aacattgaac ttagcaaaca
    33901 agtattgacg gtcttggact gggaaacgtt ggtccaactg gtcaactgct cccacctcga
    33961 taatgccttc cttgatgaca aaatccatga catgtccacc gaaggtcaaa tcatctgata
    34021 tgaagtgcag atggtagcct gccacactga ccccatggaa aatctcaggc gtccagaaac
    34081 caacaatagt ccccgcaaca ttgtcacgac tatattccgg ttgatgggtt gcgacatcag
    34141 caaacttagt atcgggtgtt gacttaggaa tcatacgcac atgcatatgt gaaaattctc
    34201 cccgaatctt gatagaacgg aaaagatttt ccccatcata ataagactca attcgttctt
    34261 tcaattcctt gtctgtcatc tcaaagcgct ggcggaaaat gacctctgcc tgatgcggta
    34321 ccactgcagc gtaaggaata agggcatctg gtgacacttc cacaatttct ggctggtctc
    34381 ctgacccttt ggcctgataa gccttgccat ccaagacaat caattcccca tcaatagaat
    34441 ccaaggttcc caaaccaagg tcaccatgct ctagcaattc tcccactgtc atggtaccac
    34501 cataaaggcc agccatcaag gctccaagag tattgtattg aaataatttc actggttcct
    34561 gcacgttctt atccattcac tttcttgtct gttattctat aaatcttact cctaagtata
    34621 ccacatttgc ccctagatgt gaacgagaga aacgctctag acattgccaa gaaggaaaaa
    34681 aaagggtaca atgtaacaaa atcaagggag gtctggaatg aagaaacaaa gcaagtacaa
    34741 agaggtcgtt tcctatctga aaaatggtat cgagtctgga cgatttccga cgggtagtcg
    34801 cctgccttct atccgtcaac tgagccttga ctttcactgt agcaaggaca ccattcaacg
    34861 agccctgctg gaattacggc acgaacaata cctctatgcc aagccccaga gtggctacta
    34921 tgtcctagaa caagggcaac accaggacct agaaatcgag gttaccgacg aacatgccag
    34981 tgcctatgac gatttccgac tctgtgtcaa tgaaaccttg attggccgag aaaactacct
    35041 cttcaactac tatgataatc aagaaggatt agaagaccta agacaatcca ttcacaaact
    35101 cctctttgag caagctctct actgcaaggc taaccaacta gtactgactt ctggaaccca
    35161 acaagccttg tttatcctct ctcaaatatc ctttcctagc caagccaagg aaatcttggt
    35221 ggaacagcca acctaccatc ggatgaatcg cctcttgatt gcacaggggc tggactatca
    35281 aacgattgaa cgaggcattg atgggattga cctgaaggag ctggagcgac acttcaagac
    35341 aggaaaaatt aagtttttct acaccattcc ccgatttcac tatcctctgg gacattccta
    35401 ttctgagcaa gacaaacgat ccattcttaa cttagctgcc aagtatgatg tctatatcgt
    35461 agaagacgat tatctgggtg atttggactc caagaagggc caaaccttcc actatcttga
    35521 tacagaggag catgtcattt atatcaagtc cttctcgacc agcctttttc ctgcccttcg
    35581 tattacagca ctcattcttc caaatgctat caaagaagca tttgtggcct acaaaaatat
    35641 cctagactac gacagcaacc tcattatgca aaaggccctg tcactctata ttgacagtca
    35701 attgtttgaa aaaaatcgtt tggctcgctt gaccaatcat gaatcttacc aaaaacaaat
    35761 cgaggaaagg ataactaaaa caccttgtcc ccttcctcat tatcccctac acgatggttt
    35821 attgctagac ctgagacagt atcctaaaat cgccagtctc aaacacagtc aaccgggctt
    35881 ggacttcttt gaagaggcct atttaagtac ctgtccttat caatttgcca aggtgtccct
    35941 agacaatctg gaaaatgttt taaactattt aaaagcagaa ttggaatgac atccaactct
    36001 gctttttctt attcttcaac ttcagttaga cttgctgctt tttcaagata gctttggtaa
    36061 attccttcat ccttaagttt ttggatgacc ttgtctacca cttctttcaa atcatcacta
    36121 ttttttctaa gagcaacggc attggcgtcg ccgtccttca tcttcaagct gacagttgcg
    36181 acagctaagc cagcgttttt agcagcataa ctaagtgcaa caggctcatc catatgaaca
    36241 gcatctactt ttccagcctg caattcattg actgcttcac ccatattagt tagggaagtt
    36301 aattgagctt ttggcaattg ttccttgacc attgattctg gaacagtccc tttttgggct
    36361 gcaatattag cactttctag gctagttaaa tccttgtatt tttccacatc agccttacga
    36421 accaagaaac taatcttgtt ttcatagtat gggattgaaa aatcaaagac ttcttttctc
    36481 tcgtcagtag cactaattcc tgcaactgct aggtcagcct taccagtttg aagactggtc
    36541 aaaacattgt caaaactcat gcttgagatt tccaacttaa ccccaagttc atcagcgata
    36601 gcctgagcca tgtcgatgtc tgcaccgact acctggttct ttccatcaac caatgattga
    36661 aattcaaagg gtgcatagtc aggactggtt gccacaacta atttcccctt ttgcttaatt
    36721 gcctcaacag ctgactgaga accattagaa cctgactggc aagctactaa gaagaaactt
    36781 gcaagaaaat tacataaaac aaatatccat ttttttgatt tcataaataa acaacctctc
    36841 tgttaatttt gtataattat aacgatatcc aagttacttg tcaagtgttt tttaaatttt
    36901 tatctcaaaa atattttttc gttcaaaaaa aggagccatc agttgatttc aagctccttt
    36961 ttatacagaa ttaaactatt ttatagttcg acaatcttac ctgtttcaaa gtagacaacc
    37021 cattcacaga tatttttagc atagtcaccg atacgctcca agtaggaaat aacttggaaa
    37081 taatcacgac ccgtaacaat ggcttctgga tttttcttaa tctcttcagt cgcaaggtca
    37141 cggatagttt caaaatagtg gttaatttgc tcatccatgg aggccacccg gtatgcgtcg
    37201 tcaacagaac cattaagata aagatcaagt gctgcttcaa caacgctttt aacttcacgt
    37261 cccatttttt taatttcttc ctctacagct ggaatacgtt cttccccctt catacggatg
    37321 gttgcctggg caatggctac agcgtgatcc cccatacgct ccacatctga tacagcctta
    37381 aggacagtca agactgtacg caaatcttga gagactggtt gttggagtgc gatcatttca
    37441 aatgatttct tttccagttt cacttcgtat tcatttactt ctgcatcatc ttcgatgacc
    37501 tcttttgcca ggtcacggtc atgcgtgaca aaagcacgta ccgtacgatt gatttgtgag
    37561 agcacttctt gtcccatagc gtagaactgg ttatgtaatt tctctaaatc ttcttcaaat
    37621 tgagatcgta acatctttca tctccttatc caaattttcc tgtaatatag tcttccgttt
    37681 ccttgtgttg gggatcaagg aacatctgct tggtatcatt aaattcaatc aaatctccat
    37741 ctaggaaaaa tcctgtctta tcagagatac gtgaagcttg ctgcatggaa cgggttacca
    37801 gaagcatggt gtacttgtct tttagaccat acaaggtttc ctcaatttta ccagctgaaa
    37861 tcggatccaa agccgaagtt ggctcatcca agaggatgat tttaggacta gttgccaaga
    37921 cacgggccac gcagacacgc tgctgttgac cacctgacaa tccaatagct gaatcatata
    37981 gacgatcctt gacctcatcc cagatagagg caccttgcaa ggctttttct acggcttcat
    38041 ccagaacctg cttatcctta attccattga tacgaagccc gtagacaaca ttctcataga
    38101 tagtcatagg gaaaggatta ggttgttgga aaaccattcc gatttcctta cgtaattcaa
    38161 ccgtatctgt acgcggactg tagatgttgt gaccattgta caccacggat ccagttgtgg
    38221 tcacctctgg attgagatct cccatgcggt tgagagactt gaggagggtt gacttccctg
    38281 atccagatgg accaatcaag gctgtaattt ccttaggttg gaaagatagg gaaacactat
    38341 tcaaagcctt ctttttatta taataaacgg acaggtctga tacctgtaaa atcgcatctg
    38401 tcatacggtt tcctttctaa ccaaagtgac cagatacata gtcattggtg gactgtagct
    38461 tggcattttg gaaaatagtt gcagtcttgt catactcaat caaatcaccc aagtaaaaga
    38521 agcctgtata gtcacttgca cgagcagcct gctgcatatt atgcgttaca atgatgatgg
    38581 taaagttttt cttgagctca aacatggtct cttctagttg catggtcgca atcggatcca
    38641 aggctgaggc tggctcatcc attaagagga tatctggctt aacagagatg gcacgagcga
    38701 tacagagacg ttgttgctga ccacctgata aggtcaaggc tgacttgtgg agatcgtctt
    38761 taacctgatc ccagagggca gcctgacgaa gggaggtttc tacgatttca tctaggactt
    38821 gcttatcctt aactccagca cgttcatgcg caaaggtaat attacggtaa attgacttag
    38881 caaatggatt ggggcgttga aaaaccattc caatgtgttt acgcatttca taaacgttga
    38941 tttctggacg gttgacatca attccacgat agagaatctg cccagttact ttagcaatat
    39001 caatggtatc attcatgcga ttgagactgc gtaagtaggt agatttcccc gatcccgacg
    39061 gaccaatcaa agctgtaatt ttatttcttt caaattgcat atcaatcccc ttaatggatt
    39121 catttttacc atagtaaaca tggacatcct tagtagaaag ggctactttt tcttcaggaa
    39181 aggtaaggat atgcttctca tcccagttat atgttgacat ggcttctcct ttaggcagcg
    39241 gttaatttct tgtgtagata gcttccgaac ttacgagctc caaagttaaa aatcaggata
    39301 aagatcagga gcacagcggc agaacctgct gatacaatgg ttccatctgg aatagtgcct
    39361 tcactattga ctttccagat atggacagcc aaggtttctg cttgacggaa gatagagatg
    39421 gggctagtca cactgaggat attccagtta gaccagtcaa gagctggcgc cgattgccct
    39481 gctgtataga tcagagctgc agcttcgcca aagatacgac cagatgccaa gacgacaccc
    39541 gttacaatac ctggaagcgc ttctggaata acaacatgaa ccactgtctc ccagcgagaa
    39601 atcccaagag ccagaccagc ctcacgttgg gtatggtgaa cgtgtttcaa actatcctct
    39661 acattacgcg tcatctgagg caagttaaag actgtcaagg ccaaggcacc tgaaatgatt
    39721 gaaaacccat actcaaactg gactacaaag atcaagtaac caaagagacc caccaccact
    39781 gatggtaaag aggacaagat ttcaatacaa gtccgcacaa agttggtaac aggacctttt
    39841 ttagcatatt cagccaagta aatcccagct cccatagaaa gaggtacaga aataatcaag
    39901 gtaatgacca ataggaaaaa ggaattgtaa agctgaatgc caatcccacc acctgcttga
    39961 aaagcagaag accttccagt caagaaagac caagagatat ggggcaagcc ccgaaccaag
    40021 atatagagaa ttaaggaagc caagattgtc acaatgatgc tagcaatcgt atagaggaca
    40081 gctgttgcaa gtttatctaa tttcttagcg cgcataattt ttctttcctc tttctttcgt
    40141 aatcaattta atcacactgt taaaaactaa actcatcaag agcagtacca aggccagtga
    40201 ccagagaaca ttattattta cagttcccat gacagtgttc ccaattccca tagttaatat
    40261 agaagttaaa gttgcagctg gtgtggtcaa ggaagttggg ataacagctg agtttccgac
    40321 aaccatctgg atagctaaag cctcaccaaa ggcacgcgcc atcccaaaga ccactgcagt
    40381 gaaaatacca gaacgggccg ccttcaagat cacacgccag atagtctgcc agcgagtggc
    40441 tcccatagcg aaactggcct cacgataata acgaggaacc gcacgcaagc tatccgttgt
    40501 cataaaggtt acggtcggca aaatcatgac aaagaggacg gaaatccctg acaaaatccc
    40561 aaaaccagtc ccaccaaaga cactgcgaac aaagggaacg acgacttgca agccaataaa
    40621 tccgtacact actgaaggaa tcccaaccag gagttcaata gctggttgca aaatcttcgc
    40681 cccttttggt gatacttcgg ttataaaaac tgctgcacca atagcaaagg gtgttgcgat
    40741 aagggctgag agaatggtaa cgataaagga acccaaaatc ataggaaggg caccaaattc
    40801 tttactagaa ggattccaag ttcctcccaa aagaaagtca aagatattca caccattgac
    40861 aaagaaggtc gacaagcctt tttgcgctac gaaaaccaaa atcatggcca caaggatgac
    40921 tatcaaagaa agacaggcaa aggtcaaacc ttttcctaat ttctccagac gagaattctt
    40981 tgatggaagc aacattttct tagctaattc ttcttgattc attattgtct cccttccaac
    41041 actgtcacag ttccggcagc atctttttca accttcattt ccttaatcgg aatatacttc
    41101 aatcctttga caatcccttc ttgggtctca tccgagagaa caaaattgag aaattctgca
    41161 gccaactcat tgggctgccc caatgtatac atatgctcat aagaccacaa gggccaatta
    41221 ttgctactta tattttctgg acttaagtca tagccattca acttcatgct tttgaccgaa
    41281 tcatctatat aggtaagaga taaataagag atagctcctg gactttttga tacgattgat
    41341 tttaccgctc catttgaatc ctgctcctga ctttgcatgg cagactgacc ttccataatg
    41401 acagtatcaa aggtagcacg agaaccagag ccggctgccc gattgataac agagatgggt
    41461 aagtccttac caccaacctc tttccaattg gttacctcac ctatgaagat ttgacgaagt
    41521 tgctctgtcg ttaggttatc aacatcaacc tccttattga caatcagagc caagccagct
    41581 accgcgacct tgtggtcaac aagagcagaa gcatcaattc cgtctttttc ctcagcaaat
    41641 acatctgagt ttcctatatc aactgcccca gactgaacct gggacaagcc tgtaccagaa
    41701 cctccccctt ggacattgac cgtttttcca acatggatcg tgccaaattc atctgccgct
    41761 acttcaacca agggttgcaa ggcagttgag ccaacagccg ttatggattc tccacgatca
    41821 atccagctag cacagcctac taaacaagcc gtcagccaaa aagcgataag agacagagca
    41881 agcttttttc tttttttcac tgtttttctc ctcgaaaata attatgaata ctgtgaattt
    41941 tttaagtagt tctttatgag ttgacgcatg aattcttacc aaatttctgc gcaattgatt
    42001 atttatataa tataggctat attactcttt cctaacctcc ttttttcata tgtggataaa
    42061 atctcttgtc tatcccttcc cccattgtca cccattatag tcatttcgtg tctctttttc
    42121 ccctttttaa tgcaagggaa attactctcc ttagatgata atccaaaagc tagaaaggta
    42181 tctcaaacct ctctactctc ccagactagt ttacaactaa aaggaaaaga ttctatttta
    42241 tgagaaatct agtttacaag cggtaagaac gctaataact aaacttcttg tactctttga
    42301 aaatctcttc aaaccagtgt tttgagctat ctatggctag cttcctagtt tgctctttga
    42361 ttttcattga gtagtaaaac tacatgtaat ggcaatcaag atatcaagaa tcatcctact
    42421 aaaaaaatcc atactttcac tataacatag aataagatat ttgactagca ttttcatttg
    42481 aatctgaggc cttttggaaa ataatttttc aaaacatttc cagtaacctt tgcaaagccc
    42541 aagccattgc ctttaaccaa aacttggtac caaccatttg gcagactttc tgccagctga
    42601 acgatttctc cagccgcata cttgacaaac gcttcttggc caatttcaac cgactgttcg
    42661 acctgactcg gtttcaaggc taaaccaaga gcgaaactgg gctcaaagcg tttcttctta
    42721 aaagtgccca gatgcagtcc attgcgagca atcttgagct tccctaaatc tggcaaaagt
    42781 tctggcaaga gataaagctg gtctccaaaa gtctgcaaga tacccggtag attgaccttc
    42841 aaatggtttt gggcaaattc ctgccacaag gcaacttgtt cacggctgag gttactctta
    42901 cttgccttaa atttaggagc tggattgtta cccttaaact gtagatgggc aacaaactga
    42961 ccctctccct taaactgatg agggtacatc cgagccgttt ctggcaggtc aataccagct
    43021 accattccat tgatatgctc tactggcaac aagtcaaaat catactcttc cagcaaccaa
    43081 ttgacaatct cttcgttttc ctcgggtgcc caggtacagg tcgaataaac cagacgacca
    43141 ccttcagcta acatggtcac tgcatcctcc agaatttctc tttgcaagct agcacattga
    43201 ctcggataat ctaagctcca atagtccata gcatcaggtt gcttacgaaa cattccttca
    43261 ccagagcaag gggcatcaag aacgattaag tcaaaatagc ctttaaagac cttgaccaag
    43321 cggtcggcag attcattggt caccacgaca tttgtcgctc caaaacgctc catgttttca
    43381 accaaaatct tagcccgttt gcttgaaatt tcattggaaa caagtagccc ctcccctgct
    43441 agataggctg ccagttgagt tgatttgccc cccggtgcag cagccaagtc caagaccttc
    43501 ataccaggac tgggttgagc tacttgagcc accatttgag cagcaggttc ttgcgaataa
    43561 actaaacctg tagcatgctc aggcgatttc cctgaaacct tcccatagtg gccccaaggg
    43621 gtttgagtaa tggcatcaga aaaggaaagt tgctcttctt ttaagggatt gacccgaaag
    43681 gccgaaaccg cttcctcctc aaaagaggca agaaaatctc ttgcctcatc tcctagtatc
    43741 tctttatatt tttcaacaaa tccttctgga aattgcattt aagttctttt cctttcgtaa
    43801 atataggact gaatttcctc ctgcatctca agaggcacca tcatgaccgg ctgtctggtt
    43861 tgaaaatcag gagcttcacc aaaaagggtc acaacccgat agcccagact ttcccctaaa
    43921 atactagctg cggcataatc ccatggttgc agataagtga gataggtcaa caaacgccct
    43981 gacaaaatct tggcaaaact aatggccgca cttccataga cacgaacacc aagaaccgct
    44041 cggctcaaat cagccagccc ccattcattg gtttccagca taccactatt ccctgcaatg
    44101 agaaaatctc caagtggttt agttttaaaa ggagctaggg gcctatcatt tagacaaact
    44161 ggaaattccc caccaccgtg gtaacaatcc cctttgacca catcataaat cagaccaaac
    44221 tgtccctgac cattttcaaa ataagccatc ataacagcaa aatcttcctg ctgggctaca
    44281 aaattattgg taccatcaat gggatcaatg acccaaacct tgccctcttg aaccgaggct
    44341 cgcagacaac cttcttcagc acaaatctta tcctcaggat aacgggacaa aatctcacca
    44401 accaagagtt cctgaacttc tttgtccagt ctggtcacca aatctgttgg agaggacttg
    44461 gtttcaacac gcaagtcttc ctgcatatgg tcaagaatgt actgacctgc tttcttaaca
    44521 agctctttag caaattcaaa tttactttcc aagagaaatc tttccttccc ctttttcttt
    44581 ggcagcctga actgctcggt aaagtgaata gccactaacc gtttcaaatt ctcgtcccaa
    44641 acgtttctct tcgctcttgc ttggcacgac cgctttgaat cccttatagg agtccagtaa
    44701 ctttttagcc tctactttgc cttcataggc agcttcaaca tcattaaaaa aagaaagcac
    44761 tgaagcaagt tcttcagtgc tccacgacaa atctagtggg taactatact gtttgttcat
    44821 taactaatac cagctctcat tcttgcttct tttagttctt gcttacgata actacgaggg
    44881 agaaaagcac gaatctcatc ttcattaaaa ccgatttgca tacgcttggc atcaataata
    44941 attggacgac gcaaaagact aggatactgc tcaatcaaat gaagcaattc cgataccgaa
    45001 atactctcta catcaatatt caatttttga aaaatttttg aacgagttga aatgatgtca
    45061 tcagtaccat tttcggtcaa ggaaaggatg tgttgcaatt cttttcttgt taaaggactg
    45121 gtcataatat tgtgttccac aaagggaacc ttatgttttt ctaaccaggc cttagcctta
    45181 cgacatgatg tacagctcgg tgatagaaat agtgtaatca tgcttttctc ttcttatcta
    45241 tactttgcta cttctattat acaaaaaaat aaggcgcttg actagggatt tttagaaaaa
    45301 aagcctattt tttcaagaaa aataggctgt ttgcgaacga ttgacacaat tggatttggt
    45361 taattcactc ttaacgatgg ttttaaacga tatatatttt tatatatgca aattaaaaac
    45421 atctttcatt tcacttccta cgacttttca gacacagata gccaaagaag ctttcataga
    45481 gggcaaaaaa gaggaggaag gcatgaagaa agaaggtctc tggcaaaatc ataataacag
    45541 gatccttggc tggatcaaaa agccaggtat catctcccac aaagagaatt tgatggaaaa
    45601 gagtaaagaa ttggtcaaaa ccaatcaaaa ctcccccaag tccaatcatc acaggtaaga
    45661 ctactagagc caggagactt tttcgataaa gagacaaaaa gtcctttttc acaatcctat
    45721 tgacaaagac atagaaactt ggcagtgtca ctagagctac tagctgaacc aaatgaaaga
    45781 gattcttgac cactgcgaaa tggtgcagac cagctgctga cgaacgaaaa tcaggcatct
    45841 gtaagacctg actaaaagga ttggtcagat aattcatcaa gatatgaaaa ttgtattgaa
    45901 tggtttctgg ttttagatag actcgattcg ttaagtttag ccactgaatc tccataggat
    45961 agaaaatcca agccagataa atggtcagaa ggattgagag ggagaggaga aagagcatag
    46021 agccccaaaa ggtcaattta gttttcatca aaatcccact ccgcaaggct agaaaccaca
    46081 tgtgtcggtg cgattggcag gccagctact tcttctgcct tggtaaaacc tgtcgtcacc
    46141 aagagcgttg gaatgccatt gtcaatccca gcccggatat cagtcaaata attgtcccca
    46201 accatgatta actcttcacg ttccaaacct aagtgctcaa ccgccttgtc cataatgatg
    46261 gcatttggtt ttccgatata aaccggcttc actcgtgtcg ctacttcaag cagcgtaatc
    46321 agtgagccag cacctggcaa aagaccgcgt tccgtcggga tgttgaggtc aggattggtt
    46381 ccgataaaat gggcaccctt ttgaatagca agagttgctg tggcaaattt ttcatagtcg
    46441 acttgccaat ccagaccaac taccacgtag gcaggttttt ccttgtcttc cacataacca
    46501 gccgccttga tggcttcctt gagtcctgct tctccgacga catagacggt cttttcaagc
    46561 cccaaatcat tcatatagtc gatggttgcc aaagtcgctg tgtagacagt cgataggggc
    46621 gtatcgatat taaaattctg agccaacatc tccttaacac tctctggagt gcgggttgta
    46681 ttgttggtta caaagagata gggaatgtcc cgcttttgca attcatgaac aaaagtctct
    46741 ccagcaggga ttcggtcttt ccccttataa atggttccgt ctaaatcaat taaatagcct
    46801 ttatatttca tctatttctc cctaagcctt ttttatttct tgccaagtaa tgattgcttg
    46861 ggcattgata accccatcac ttgtaatttc atgcttgctt tccagtccag tccgttcaac
    46921 agccgatgta atcaccccac ctggtcgaac ttccttgaca tacttgaggt tgattttctt
    46981 gggaatatag tgggtcaaaa aatccgctcc catgacctca aaaatccagt ccaagtattt
    47041 actgttattg acatgaccat tcatatccaa gtcgtaaaaa cgaacatggt aatccttgct
    47101 gatcggttct tccaaggact catacttcgg tccacggata agttttttat caaaatcaga
    47161 ctggtaagga gccacaatct caggttcaac aacatggact tttcgactgt cgcggtccat
    47221 gagaacaaag gtcgccatca tgtggatgag ctcctgctcc gcttcattat aaatagtaaa
    47281 gcgacggtag caaaaaagtc gattgtagct caaggcttcc gtttcgatgg taatttcttc
    47341 cgcaaaacga ggcaaacgaa ccacctcaat atcatattct acgataatcc agaccagatt
    47401 atattcttcc aaaatggcct tatcactaac tcccagttca atcgactgca tccctgaaac
    47461 ttgcagtgac agcaaaatca catctggaag tttgatatga ccgttcatat cagccatatc
    47521 aaaaggaatt ttcattttca tttgataagt taagcccatg atcctactcc aaaataaatc
    47581 gttctgctac agtatctccc aaaaagagac ctctctttgt catgcgaacg tggtcaccct
    47641 caatctgcat gaggccttgt tgaaccaaat ctctgacaat ttctccataa agtccagcaa
    47701 aagactgtcc aaatttttcc tcaaatcgcg ccatggaaac cccggatttc ttgcggagtc
    47761 ccaagaacat ttcttcttcc atttgctcct tttgactcag gtgatcttct gtaatacaag
    47821 cattgccttc ctcaaccgca ctgagataat gacgaatggg accatgattt ttatagcgta
    47881 ctccattgac ataaccagat gcccctgcac caataccata gtattcagca ttgtcccagt
    47941 acatgagatt gtgacgactt tcaaaaccgg gtttggagaa attagaaatc tcataatgct
    48001 caaaacccgc tcgctccagc tctgcaatga tgtactcaaa catctccgct tctagttcct
    48061 ccttaggcag aggcaatttc ccacgtcgca tccggttcat aaagaccgta tggttttcta
    48121 aaatcaaact atacaaactc atgtggggaa tatccagtcc aatggcttta gccacatttt
    48181 cctttacttg ctccatggtc tgaccaggca gagcataaat caaatcaatg gagatgttgt
    48241 caaaaccagc cagtttcaga cgatcgatat tttcataaat atccttctcc aaatgactgc
    48301 gcccaatctt tttcaacatc ttatcatcaa aggtctggac acctagcgaa acacgattga
    48361 cagccgaatt tttcaaaaca gctatcttat ccgcatccaa atcgcctgga ttggcttcaa
    48421 tggtcaactc ttccaagaca gacaagtcca agtttttagt caagccattc agtaacacct
    48481 ccagttgcgg agccgacagg gctgtcggtg ttccaccacc gatataaagg gttgacaact
    48541 tttcaatatc ataagaacga aactcttcca gcagatgctc taaatagctg tcgactggct
    48601 gatttttgat gaagaccttt gaaaaatcac aataataaca aatctgggta caaaatggga
    48661 tgtgcacata ggctgacgtt ggttttttct gcatagtaat tattatacca caaagactag
    48721 attccagata aaaatcacca tccccagata catagtccgt ccggagatgg tgatggttta
    48781 ttcttctgtt atatcaatca caatctcttc tgagtcatca agagcttcgg ctttttcttg
    48841 ccattgttcc ttgagattat ttaattgatt ttttgatgct tctgtcgctt gaaaagcata
    48901 ggatttagct tgagcaagta tactgtccac agtgatttca cctgactcaa cctgttcttt
    48961 tgttttcaga acaaaatctg tagcctgctc cttaacttct gtcagttttt cacagacttg
    49021 ctccttggca tactccggat cttctctcaa atcatctaga aaatcttgag cctgactgca
    49081 aacttgtttg cccttatcac ttgttaaaaa caaggcaaga gctgcacctg aaaccgttcc
    49141 taaaaggatt gaggataatt tacccataag gattctcctt ttttattttt tgaaaaattt
    49201 acttgcaaga cgaagagctg acagacttgc accagtcttg agtgtttttg aaccagctga
    49261 tgaagctttc ttgctcaaga cacgcgcatg gtcattgagg tctgaaacag atagagataa
    49321 atctgcaaca gcactgaaga gtggatcaat cgtagccacc ttgacattga tatcatctgc
    49381 caagacattg accttagcca acaactcatt ggtgtgatgc aaggtcacat ccacatctga
    49441 agtcaaggtt ttaatcgtct tttctgtttc atcgatgaca cgaccaagct tttgtacagt
    49501 aatgatcaga tagaccaaaa agacaatcaa agctagggca acaagaatat atgcaacttc
    49561 taacatttat ttttcctcct ctgtaatata gtaaggggcc ttctttcgat tttgataaat
    49621 aacgatcatt ataccgagac cgataaggac aactgacagc cattgggaca ctcgaaggct
    49681 gaagaacatg agactatctg ttcgcatacc ttcgatgacc atacgaccga aaccatacca
    49741 aatcaagtaa aaggccgtga tatgacctcg tctgagactc ttccatttcc gtctaaaaat
    49801 cagaatcaaa gcaaagccaa gcagattcca tagagactca taaaggaaag tcggttgacg
    49861 gtagctcccc tcaatataca tctggtcacg gataaagcca ggtagataat ccagattatc
    49921 cactgttgca ccataagctt cttggttaaa gaaattaccc caacgcccca aactttgagc
    49981 aatcataacg ctaggcgccg caatatctag aaaatcccaa gtattgatga gtttacggtc
    50041 agcaaagata tagagcacaa gagccccagt tatcaaacca ccgtaaatgg ccaaaccacc
    50101 attccaaatg gcaaaaatct ctcctaaatt ctgactatag taatcaaatc ggaaaataac
    50161 atagtagaga cgagctccta aaatagccaa gggaaaggct actaagataa aatctaaaat
    50221 atcgtctggt atgatcttct ttctaggtgc ttctttcatg gtcaaataaa ccgcaagaat
    50281 caagcctgtc acaatacata aggcatacca acgaatggct aggggtccta gttgaatagc
    50341 aattggatca agcattttgc acctcatttc gagcaatgag acttgtcagt cgttcgtcga
    50401 acaaacgggt cgcatcaaag cccatttcct tggcacgata attcatggca gctgcctcaa
    50461 tcacaacaga gatattacga cctgttttaa ctggaatacg aatacgagga atggctacgc
    50521 cagaaacttc aagttcctct gcattgtttc caagacgatc aaaggtctta tgtgtatcgt
    50581 aattttccaa atagacagca agctgaacct gtgaagaatc cttgacagca ctcgcaccgt
    50641 agagactcat aacatcgata ataccaaccc cacgaatttc aatcaagtgt ttcaaaattt
    50701 cagctggttc accccagaga gtaatctcat ccttggcaaa gatatcgaca cggtcatcgg
    50761 ctaccaaacg gtgaccacgt ttgacaagct caagacctgt ctcgctctta ccaattccac
    50821 tatctccctg aatcaagacg cccatcccat aaatatccat caagacaccg tgcacactgg
    50881 tacgttctgc caaacgagaa tccagatagc tagataactc tccagataaa cgactggtag
    50941 ctgcacggct ggttaaaata gcaatcttac attctctagc agcctttaac atctcctctg
    51001 gaaccaccaa accacgagca acaatgactg ctggtgtctc aggtagaaac atttttttca
    51061 aaacttcata acggctgttg gaaggcatgc tgatcagata agaccactcc ttcatcccca
    51121 aaagttgaat ccgctctggt gtatagtagt caaaatagcc tgtcatttca agaccaggtc
    51181 gcgtaatatc cgctgtattg atttcctttt caagcaactc tggttcacca tagacaatgt
    51241 ctagtctaag cttttcaatc acttctttta ctaaaaccga catcatttcc tccttcaaat
    51301 gagttcttct ctctattata tcaaattgta ggaggaagtt tcagattttt gcaggctttg
    51361 gagtatttat ttaaaagaaa aaggactaga tttctctagc ctttcatttc aattagaatt
    51421 ttttacgttt acgagctact tctgatttac gtttacgttt tacagaaggt ttttcataga
    51481 attcacgttt gcgtgtttct tgaagagtac cagctttagt aaccgcacgt ttgaaacggc
    51541 gaagtgcgtc gtcaagagat tcattcttac gtactactgt tttagacatt tttttcactc
    51601 ccttcaagtt caatatctat ttcattctac acgtaaaagg aataattgtc aagcaaaaat
    51661 gcggtttaat tttgattttt tcttccattt atacaagagt tgaaagcgtt caatagaaca
    51721 ttgctttttt atttttcgag taagctaagc gcttcagcat ctgcgatgat ggttacatca
    51781 gggtgatttt gaaggctact tgcaggtagg ttctcagtca ctgggccaga tactgttccg
    51841 gcaatggctt ctgctttcga ctcaccgtaa gcaaaaagaa taatagactt ggcatccaaa
    51901 atgtttttaa tccccattga aatagcttgg gttgggacgt cttcaatctt ggcaaagaag
    51961 cgtgcattgg cttcgatagt agactggtca agttctacta gatgcgtttg actgtcaaat
    52021 ggagtaccag gctcattaaa gccgatatgc ccattgcgac cgattcccaa gatttggagg
    52081 tcaactggat ggtcagccaa aatttggttg tagcgttcaa cttcagcttc agcattgtcc
    52141 ttaacaccac gaggcaagaa actttcttta aatggttttt ggttgaacaa gttttcttgc
    52201 atgaagtaac gataagattg tggattgtcc ccatcaaggc ccacatactc atcaaggttg
    52261 acactggtta gatttgaaaa atcaaggtca ctctcaacaa tttccttgta aaattcaagt
    52321 gggctacttc ctgtcgcaag tcctagagtt tgagctccat tagccaattt ttccttcaaa
    52381 atctcaaaag caacttttcc accttgaact tggttttcaa ctttaataac tttcatcttt
    52441 tcatcctcca tttctgtcta tatttattat atggtataga ccaatttttg tcaagtgaaa
    52501 acgatttaat ttctaaaaaa agagcgtcca aacttgaaac gtgttataat ggataggatt
    52561 agaacttaga aagaatgaac aaatatatga atacagctga ttttgatttc cacttgccag
    52621 aggaattgat tgcccaaacg ccccttgaaa aacgtgatgc ctccaaactc ctcatcgtca
    52681 accgtgagac aggagaaatg caagataaac acttctactc tattattgat atgctggaac
    52741 ctggtgatgc ccttgtcatg aacgacaccc gagttctccc tgcccgcctc tatggtcaaa
    52801 aagtggagac aggaggtcat gtggaacttc tcctccttaa gaacactagt ggagacgagt
    52861 gggaagttct ggctaaacct gccaaacgcc tcaaggtcgg tactcgtatc agctttggtg
    52921 atggccgcct cagcgctgtc gttacagaag aattgaccca cgggggacgc attgtccgct
    52981 ttgaatacca aggaattttc ctagaagtct tggaaagtct gggagaaatg cctctgccac
    53041 cttatatcca cgaaaaatta gatgaccgtg aacgttatca aaccgtctac gccaaggaaa
    53101 gcggctctgc tgcagcaccg actgctggtc ttcacttcac caaagaactg ctggcagaaa
    53161 tccaagctaa gggtgttcat ctagtctatc tgactctcca tgtcggactc ggaaccttta
    53221 gacctgtttc tgtggataat ctggacgaac acgaaatgca ctcagagttc tatcaacttt
    53281 ctgaggaagc tgctgccacc cttcgctctg tcaaagaaaa tggtggtcgc gtcatcgctg
    53341 tcggaaccac ttctatccgc accttggaaa ctattggttc caagtttgat gggcaaatcc
    53401 aagcagattc tggttggacc aatatcttta tcaaacctgg gtatgagtgg aaggtcgtgg
    53461 atgccttctc aaccaacttc catctgccaa aatcaactct ggtcatgttg gtttctgcct
    53521 ttgcaggccg tgaattagtc ttagatgcct accaccatgc catccaagaa cactaccgct
    53581 tcttcagttt tggtgacgcc atgtttattt attgagaaag aatttctcta attcttctaa
    53641 taccaataaa tcgctaagat attatttcaa agaacatcta caattgaaac tctagctagc
    53701 tgtagaagac gcctagtaca ttgaaattaa aatgcttccc cctagcttcg aaaatattgc
    53761 catagattgc gttgactctc caaattgatt catctatatt ttatttcagc ttcctatatt
    53821 ttcttcgctg tttgtaaatc aaaatgcaag acacatgagt agcaccatat ttgttactct
    53881 tatctgtcct ctcaagagac tattatgagt tatttcagaa tcattcacta ctttgaccct
    53941 gactctcctt agtctcaaaa tcaaagactt atactcttca aaaatctctt caaaccgcgt
    54001 caacgtcacc ttggattata tatgtgactg acttcgtcag ttctatctac aacctcaaag
    54061 cagtactttg agcaacctgc gactagtttt ctagtttgct ctttgatttt cattgagtat
    54121 taaacaaaaa gtgaacaaat ctgaattcta atgtacagaa gactaggctt gttcactttt
    54181 ttatagtcgc tataagatga ccttatctat agctttttat atataattat atattcagac
    54241 atactattat caattttgtc gcagggagga atctgttaac gcacccattc accattatca
    54301 ttgactctat agccatctat acttgtattg accgctaact cacccgatgt atttacataa
    54361 taccatttac caccaacttg gaaccattga ttgactttca tagaaccgtt gctgttgagg
    54421 tagtaccatg aaccattaac ttgtacccaa cctgttgcca tggaaccatc agtattataa
    54481 aaataccaca taccattttc ttgtttccag cctgttgttg gagcaactgc tttagctggt
    54541 tctactgcta catctgttcc ttggttagat gtaacagata caggatacga aggaatagat
    54601 gattgctcag gaacaacaac tttttcaggt tctctcgtcc ctctccttat acgtcttttt
    54661 accatctctt tagtaatttg acgagaagta gtttcttcaa ttgttccatc acgttcatct
    54721 acagtataga ttgtagtaag agtaatttac caatttctcc tacttcttct acttcttgac
    54781 ttttatcaag agttgggcca tcgagatatt ctgtttcgat tgaaatttct tggacaagaa
    54841 cttggggctt ggttcctttt ttaacaactc ttgtttgaga gtcttctttt tgacttaaag
    54901 tactctcagt tacttgtcca ctctttccat ctacatcata agttatcgtt gtaactattt
    54961 tcccattctt tcctagagta atctcttgct cctgtcctgc agaaaggtca ttgtctgctt
    55021 catatttagt agcaaatgga acaaaaactt cttcaacctt gcttttagct ggaactttga
    55081 taactgtatc cgtggcttct tttctatcaa cagtaacctg ttcggtaaca tgaccagtct
    55141 ctggattaac atcgtaggtc cttgtcgtag ttacatagcc atcctctcca tcaattgtaa
    55201 caggattttc actacggtct tttgtttcat ctttttcata acgaattcgc gtacttgaaa
    55261 ttttcttggt tactacctta ggtttagtcg ctacttttac aataatatcc ccattgtcag
    55321 cgtcatcata ctctattccc tcttctttat ctctagtatc atctctgaca tattgaatcc
    55381 catcagcagc atgaacaaaa cttgtattca gattcctcct aaaaataaag ttagcccgat
    55441 taccgcagaa ccaaaaatct ttccgagttt acgtattgca tagcgcttat tagtattaga
    55501 ttttgccatt acatcctact tctagtatag catcttttct atcaaacgtt aaacaatata
    55561 ctttatatat aaaatagact tagaatgata tattgattat tgaactaaca ctttaactat
    55621 atcgtaatca atctcatata taaaggattg cagacatctt atctaaatac atgctaatat
    55681 atttagatac aaacattcca acttgataat tttcactcat ctttcatcat tccttataca
    55741 actatgcagt ataaatagaa tagttttctc atcagaatga gactatttta atattagata
    55801 atttgataga aaaaacatga ataaaattgg ttgaatttaa ttccctaact cgatagagga
    55861 ttctatttta aatttaacaa cagctaggat tacactataa aaagtacatt accacctgtt
    55921 cggctggaga atctagtcga ttaggtccct cttagttaca agcttaaagc gattttttaa
    55981 ctaatactaa accagattaa agaaggaact agaacatctg tcttgtacta atcaatatgc
    56041 tattttaaag tttcaaataa acttggaatg ctaattatta tatttacaat aaatagttga
    56101 cttcgataga aacagaaaat agaattcaac attcttccct acttatacca taataaataa
    56161 gatccgcaag atagcctttt ctctctacac cattaacaat ggtttgtaga taggacattc
    56221 cagccttttc catgacacga cctgaagctg ggttgagact ggcataacgt gctctgacct
    56281 tttgaaaacc tgcttgagta aaacaaaagt ccaagatagc tttcaaggcc tctgtcatca
    56341 taccatgtcc ccagtaagcc ttgcctagca catagccgat ttcacagctt aaatcagcct
    56401 cgtctatctt aacaatgctg atatctccta tcacttgctc tgggttttct tttagacaaa
    56461 tggcccattt ataatagttg agattagtat aggaagcaac ccaattgcga atcgagtttc
    56521 gagtgatttc gatatcagga tggggatccc aggtaacata ggtcagattc tcagcggatg
    56581 aagcccaatt ttgaaacata gcttctgcat cactctccac aaatcttctt aaaatcaaac
    56641 gttctgtctg taatatttgc gtaccaatag atttcatgat gctacctcac ttccttattt
    56701 ctatgctcgt agtaaaaagg ctcaactcag taacagctag aaaacgagtt tgtcttctaa
    56761 tttcttgacg caatcgctgc ctggtttgaa atgcgatcta gcaagctttt tcaaacctag
    56821 tcatcgttgc aggggtaaga cgatgaaatc gaactcttcg agttaccgaa tctgcctcat
    56881 tttcttattt ctaaactcgg ggttcaagat cccccagacc ttctctcact ttcttatttt
    56941 aagatgagag gctgaaaacc tccagtggag gtttttcttg ctttttaatt tcagggcaag
    57001 aagctaaaaa ggtcccccgg acctttttac tcccaaaagt catcaaatac agtgatgggt
    57061 aagtggcgtt tgtgttggcc tttgtgccac cagttttcaa tggtcgcttg agcttctggg
    57121 ctgattgttt tgccttctag gtagtcgtca atctctgcgt aggtgactcc aagtgcgact
    57181 tcgtcagcta ggcctggttt atcttcttct aggtctgccg ttgggatttt ttcataaagg
    57241 gctggctctg cgccaagttt ctgcaagagc tgttttcctt ggcgtttatt gaggcggtaa
    57301 agagggagaa tatccgcacc gccgtcacca aacttggtaa agaaacctgt gatattttcc
    57361 gcggcgtggt ctgttccaat gaccgctccg ctatgggaac cagcaagggc atactgagca
    57421 atcatacggc aacgtgcctt gatattcccc ttgttgaagt ctgaaacagg actacctgtc
    57481 gcttcaactg cagctgtcat ggcatcagct gattccttga tattcacaac caagctgaca
    57541 tctggctgga tgaaggctag agctttttga gcatctgctt catcagcttg cactccgtat
    57601 ggcaggcgga cagcgataaa tttgtagcta tcgtctcccg tttcagctcg cagttcttcc
    57661 atagctaatt gcgccaaacg ccctgccaag gttgagtctt gtcccccaga aatccctagt
    57721 acaaaggttt ttaggaaggg atgttttttc agatatcttt ttaagaaatc aatagaacga
    57781 cggatttctt cctgggcatc aatcactggt ttgacaccca gctcttggat aatcgtttct
    57841 tgcaaactca ttcttcttct ccttcaccaa gggcttcctt gcgcatctta tcaatcaagt
    57901 ccatcttatc ttgccataca tcacgcgcca aatccactgg atagtgctgc ggattgagca
    57961 cacgcttata ctcatcccac aacttgtcaa attctttacg ggcataatcc tgaatgtcag
    58021 tcaaactagg caagttgtaa actaatattc cttctttgaa gatatccacc aagagaggaa
    58081 cggcatcaaa attacgaacc gtcttcttga tgtatgtata ggtcggatgg aacatcttga
    58141 tttctgtcat gtcgctaata tccacaccat cataagtgat atagtcgcct tctgacttgc
    58201 ctttttcacg actggtaatg cgccacacct gcttcttacc tggcgtagaa actttttcag
    58261 cattattaga cagcttaatc gtattgcgca tctgaccagt ttcatcttcg attgcaacaa
    58321 tcttgtaaac tgcaccaaga gctggctggt cataggctgt aatcagcttg gtacccacgc
    58381 cccagacatc aatcttggcc ttttgcatct tgagattgag gatagtattt tcgtccaaat
    58441 cattagaagc ataaatctta gcctctgtaa atccagcctc gtccagttgc tgacggactt
    58501 tcttagaaat gtaggcaata tccccagagt caatccgcac acccataaag ttaatctgat
    58561 cacccagctc acgcgccacc tgaatggcag ctggtacacc gatgcgaagg gtgtcatagg
    58621 tatccacaag aaagacacaa tttttgtggg tcgcagcgta agccttgaaa gcttcatagt
    58681 cattgccata aacctgtacc aaggcatggg catgggttcc caaaacagga atgtcaaaga
    58741 gcttacccgc acgcacgttg ctagttccat tggcgccacc aatcacagct gcgcgtgttc
    58801 cccagatggc cgcatccatt tcttgagccc gacgtgtccc aaactccatc aagggttcat
    58861 cttcgataac cgaacgaata cgagctgcct tcgtcgccac caaggtctgg tagttgacga
    58921 tgttcaaaag agccgtttcg accaactgac attgggctag aggtccttcc acctgcacaa
    58981 tcggttcatt agcaaaaacc aaatcccctt cttgggcaga acgaacggtc aactccaact
    59041 tgaaattgcg aaggtaatcc aagaacgccc catgataacc aagcgactcc aaataggcta
    59101 tatcactatc tgaaaaacgc aagtcttcaa gatagttcac aattctttcc aaacctgcaa
    59161 aaaccgcata gccgttctta aaaggctgtt ggcggaaata cacctcaaag accgccttct
    59221 tattgtgaat cccttggtca aagtaaacct gcatcatgtt gatctggtac aagtccgtgt
    59281 gcaatgtcaa actatcatct ggatacatac ttttcctact tccttagcta gaaacccatg
    59341 aaaattttca agaactttca tgtattccaa taaattagta ctattatatc acattttagc
    59401 tggattgaga aaagagtaac aagctattct ccactctcca attcatccat atcttgttca
    59461 aattttttct gagcccattc gccatagctc ttaagaccaa gattgccaat aaagatccac
    59521 ggaaggtaaa tgatataagt aatgacccaa gcagacaggt atttaaaatt caaaggattg
    59581 tgctgataaa tttctatgtt gaattgataa ttctgcaaca tcaaaagagc cgtaatagcc
    59641 aaggttagga aaaaacaacc caaaatcgta aaatgaaaac gactatagta ggtcactccc
    59701 agataacggg cacgattgaa aaagtaaaat gtccctatga tgataacgat tagcagcata
    59761 ttagaattaa aaaggcttgg tgctaatact gaaatgatat aagataggag cgacaaagcg
    59821 atgcagatat agaaactttc agagcccgct ttattgaaca gttgttcttc tctttcgtct
    59881 agtaattgat aataataaaa tctatttttc atcttcttcc tcccaaaata gttggtctag
    59941 ggttttccct aaacatctgc aaatggactg gcagagcgag agactgggat tgtatttccc
    60001 cgcttctatc aaaccaatag tctggcgcgt caccccgaca gcctctgcca gttgaccttg
    60061 tgttaaatca cgctctaccc gagctaattt taattttaaa tttttagcca ccttcgtcct
    60121 ccttatagtt ttaatactca tctactctta aaaaatccaa aaccaacaca agctatcagc
    60181 tatttattat aaccccatta atttgcactc cagtattatc ttcagccgaa aatcagtatt
    60241 ttaggttgtt tgaaataaac ttcctagttt actcttttat ttttatttag tattagcttt
    60301 agtaggatct tttaaacgag tttttattgc ttatatttct tgtttagttt agttggtttc
    60361 atatcactat tatataatgc ttttggaaat gagcctagta taagtcaaaa acgtgttgaa
    60421 tagtgaaagc aggagcatac caagatgact gattttttta ttgtatttta ggtatgcaaa
    60481 ataatactaa atgaaactaa aatgaatact tcttgctttt cttgagagtg aaaaagtaca
    60541 ataaaatgat tgtagtgatt accacaagat actgactaat agacatcgta tttgaagcca
    60601 caaatacagg taaagaataa aaaggaagaa gttcaattgc tttgataaac aaatcattac
    60661 tagtattcat catcatcgta tttaatatat tagggataaa gagtagtaaa gctactaaaa
    60721 taccaaatac caagccactt ttcaatttgt ataaataaaa aatgtaagat aatagaaaat
    60781 aaaacaggac taacaaaatt gtattgacta caatagctac tataaaatct aatcctaaat
    60841 cacctggaat acccaattta cttactatga ctagtaacac aatgccattc aaagcataaa
    60901 taatggaaac tgagatggca tatagaaaat tagacaagac gtaaatcatc cgattcttct
    60961 gtttatttaa tttcatgatt ataaccttcc tttattttga acgatttagg taaatatctt
    61021 taagcgtctc ttttttagtt tcaaaatcaa ctactttaat agagttttcg ttaaaaaatt
    61081 taaaaagctc actattttga atattcccag acatagtaat tctcaatccc tcttcctgaa
    61141 caatatcccc aaattcttgt ttggtaatga aaatgtctct atctgtagct gatgaaaaag
    61201 ctatctcaaa caagaaatta tgactatctt ttcctacttt ttgaaatgtc aaaagcccgc
    61261 tctccaagaa aagaaccctc tcacaaattt cttcaatgtc ttctaattta tgactcgata
    61321 ttaaaattcc cacgttttca tttaaagcta atttttttta gaaccgctaa aactatttgt
    61381 gatgattcaa tatctaaacc attagtcggt tcatctaaaa tcaatatatc aggttccgta
    61441 acgagagtta gaagcaaagc taatttttgt tttgtaccca aagaataggt ctttactttt
    61501 ttattaatag actgagtcaa atctaactct tggatcaaac ttctaaatct ttcttgattg
    61561 tagtcaacac catataaatt tgacaaatat tttaaattct ctaaacctgt tttagataaa
    61621 aataattttg gttcttcgat taaataccca acattatcac tgtttataat attacctgaa
    61681 gtcggttgat tattttgaac aagaattttc atcaacgtac tcttaccaac tccatttctc
    61741 cctactagac caacaatttc acccttgtta agtgcaaaat ttatatcttt gagaatgatt
    61801 gaatgaccat aatgcttact tacatttata acttacattt tttattcctc cattaatatt
    61861 catggtttgc tctttgattc ttattgagtt taaaaagttg cgcctctttt taacaatatt
    61921 atatcatata tctaacttaa tgcaatatat aaatgtcatt ttgtaaaatt ttattaacaa
    61981 aaaactctct acaacaaaat aatagagagt tttctgctta ttaatttttt tcaaaatatc
    62041 taagacatcg cttctttgtt taatttgata ggctttaata cctaagctcc gactttatca
    62101 cttaaaaaat aaaaaaggac agctaatctc ttgcgcatca agggatttcg ccattcttta
    62161 atctcgttta agtgttgtat atggagcttt gcataatttt ttaaaactaa acaactacat
    62221 taagcacgaa ctgtgacgct ggttccatct tccttataaa gattgataag cccctcttta
    62281 agagctcgaa ccatgtctcc agcttgaaca agttgtggga ctttaatagt caatagttcc
    62341 attggatttg gagcgcggtc gattttattg cctttggcat catgcaaatc ttcaatatag
    62401 gtttcaaaat gacggaaacc tggaccataa aactcaactt ggtccccttc gttaatgacg
    62461 ttccgttgac gaatggttgc tgtttgtgtc gcatcatcat aagaaaccac ttcagcgaca
    62521 aacttgtact cagggatttt acgacgagca ccaaacaact gctcattttc agatggtgta
    62581 ccatagtaaa atcctgtagc cagttcacgt tgggcaacct tccacatctc gtccaccaag
    62641 tcttgtttga tagcttcaaa cttttcagga ctttcaagat aggcatccac agccgccttg
    62701 tagcagttgg ttactgttga tacatagtga atagactcca tacgtccttc gatttttaga
    62761 ctgtccacac cattttcaat catatctgag atatggtcaa tcatagacat atcaacggct
    62821 gacattgaaa attcttctgg aatctcaccc tgcaaactct tacgttcttt cccaaatggc
    62881 atatcgtaaa ggtcgtactt ccaacggcat gactgagaac atccaccacg gttggcatca
    62941 cgcatactct agacggattg ttttgacaat tctaaccttt tagactttgc ataataactg
    63001 ttaaacagtc gaaaattacc ccagttttaa caagtagtca aaataccaga taaagtaaaa
    63061 aggggctagg aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt attgaacaat
    63121 cttgatatta tgcgttttat tgtgggaaga tttacttcat tttctcttgg aattgagttt
    63181 tctcctactt caaaactaga tgatttgcta tttaaaatag cgttccttat gattttggca
    63241 acctggatta aagcgagaaa aacaaaagag gcaacttaag ctttctttat attgcttata
    63301 tgttaatagt tttagacaaa caccacataa aataggctta tcctgtatca aagataaggg
    63361 ataaggctca aaagggtgat tttctaatga atcatgacac cgataacaag cataatactt
    63421 tttacaatca tagcattgta aagcaataat atccagcttg ctatggtaat gaatgcatct
    63481 tgtttgctta tccactaaga aaccctgtgc ctctatcatt tccttttcct ttcttaagct
    63541 cgaactgtga cgctggttcc atcttcctta taaaaattga tgagtccttc ttttaatgca
    63601 cgaaccatgt ctcctgattg aacgggttgt gggactttaa tagtcaatag ttccattgga
    63661 tttggagcgc ggtcgatttt atttcctttg gcatcatgca aatcttcaat ataggtttca
    63721 aaatgacgga aacctggacc ataaaactca acttggtccc cttcgttaat gacgtttcgt
    63781 tgacgaatgg ttgctgtttg taccgcatca tcataagaaa ccacttcagc gacaaacttg
    63841 tactcaggaa ttttacggcg agcaccaaac aactgctcat tttcagatgg tataccgtag
    63901 tagaaacctg ttgccaattc acgttgggca accttccaca tctcgtccac caagtcttgt
    63961 ttgatagctt caaacttttc aggactttca agataggcat ccacagccgc cttgtagcag
    64021 ttggttactg ttgatacgta gtgaatagac ttcatacgtc cttcgatttt tagactgtcc
    64081 acaccatttt caatcatatc tggaatgtgg tcaatcatag acatgtcaac ggctgacatt
    64141 gaaaattctt ctggaatctc accctgcaaa ctcttacgtt ctttcccaaa tggcatatcg
    64201 taaaggtcgt atttccaacg gcatgactga gaacatccac cacggttggc atcacgcata
    64261 ctcatgtggt ttgaaagagt acaacgtcca gagtatgaaa tacacatagc tccatggaca
    64321 aaggcttcaa tttcaacatc tgtacgtttg cggatctcag ctaattcttc cattgaaacc
    64381 tcacgcgcta aaacgacacg agtcaagcct agctctttcc agaactcaag ggtttcatag
    64441 ttagtggcac tggcttgggt agaaaggtgg atttcaaggc ctggtgcttc agtcgctgca
    64501 atcataatca aggctgggtc agatacgata actgctgcaa tcccgatatc acgcagttta
    64561 cggaaccact caccagcacc agcttcattt ccttcgtgca taaccatatt agccgctaca
    64621 tagaccttgg caccatactt ggccgcaaac tgcacgcctt cttccatctg ttcgaaagta
    64681 aagtttcccg cacggctacg aagaccatag gcctgaccac cgataaagac agcatctgct
    64741 ccatactgaa cagctacctt tagcttctct aaagtccctg caggtgataa aacctcagga
    64801 cgttttaatg tttttgtcat gatttctcct atttgcaata taaaagtttg acatttttcc
    64861 ttctcatttt atagtaaata aaggataaaa tcaagcctag acggattgtt ttgacaattc
    64921 taatctttta gactttgcat aataactgtt aaacagtcga aaattacccc agttttaaca
    64981 agtagtcaaa aagtctaaaa aagtaaaaac cagtatccca agatactgac tcgtaaaaat
    65041 acataaatta gtccttagat gactactccc actctgttgc tcctataatg gaaaaggatt
    65101 gatacagtct agaatatcgc ttttttattt attattctga tataaaactg ttagaattga
    65161 ttttttgttt tatacccaaa tatgaataga gacgtagcta ataagttgaa taacatattt
    65221 ggaaaaatga gaaatagcaa tattactaga acttttagaa acaatcaata tttaaaaaga
    65281 aaaaagctca aagaatttat taaaccatgt tataatagat atagctataa ttttatttat
    65341 aacaagagga tagaaatgac cgaattagaa agaaaaaatc gaaaaattag ctaagaaata
    65401 ttctgataac ttaaacatca aagttcaaga gagagttcgt gaaatggcaa atgataataa
    65461 gagccattat ttgatataca gagttttagg tatttcattt gaagaaggag aaaatatcga
    65521 tttgtatcaa aataaaggtc gttttttata caaatatgct ggttcatttt tagaagaagc
    65581 tgcagtacta tcctttaacg aaaaatttgg tacagaaaat acttaaaaag ttaacattcc
    65641 taattctgaa aatacaaaac ctaagacttt tgaaattgat tgtttagtcg gagaaaaaca
    65701 cgcatacgaa ataaaatggt gggatgcaac tacagatgga gaccatataa ctaaagaaca
    65761 cactagaata aaagttattc ataacaaagg atatatacca attcggttaa tgttctacta
    65821 tccaaataga actcaagcta taaaaattca gcaaacttta gaaacattgt ataacggtat
    65881 tggagggaaa tattattatg gagattctgc ctgggaacat ttaagagcag tgaccggtat
    65941 tgatttactt agtattctaa cagatattgc aaataaaaaa acaggggtaa aatcaaaatg
    66001 acagtattaa aaggagataa cttagaaata ttaaaaacta ttgaatcctc aagtattgat
    66061 ttaatctata tggaccctcc tttctttaca cagaaaaccc aaaaattatc taataacaaa
    66121 aatattatgt attcattcga agatacgtgg acttcgattg aggattacaa agaatttttg
    66181 tctgtaagat tagaagaatg caaaagagtg ctaaaaaata gtggcagtat tttcgttcat
    66241 tgtgataaaa ttgcaaatca tcatattaga ttaattttag ataatatctt tggagcagat
    66301 atgtttcaaa gcgaaattat atggaactat aaacggtggt ctaattcaaa aaagggatta
    66361 ttgaacaatc atcaaaacat ttacttttat tcaaagtcaa aagattttaa atttaataca
    66421 atttttacag agtattcttc tactacaaat atcgaccaaa tactagtgga acgaaaacga
    66481 gatggaaact ctaaaactat atataaggtt gataataatg gtaactatat tctagcaaaa
    66541 gagaaaaatg gagttcccct ttcagatgtt tggaatatac catttcttaa tccaaaagct
    66601 aaagaaagag taggttatcc tacacaaaaa cctattctgt tattagaaca aattataaag
    66661 attgctactg ataaaaatga tatagtttta gacccgttct gtggaagtgg aactacttta
    66721 gtagcctcca agattttgaa tagaaattat atggggattg atttatctga ggaagctatc
    66781 aatataactc agcaacgtct ggaaaatgtt ataaaaacaa gttcaaattt attgaataaa
    66841 ggaatcgaag catatagaac caaaactgag gaagaggaaa acattcttaa attattacag
    66901 gcaaaaattg ttcaaagaaa taaaggaatt gatggttttt tacctaaaca ttttcaaaaa
    66961 aaaccgatac ctataaaaat tcaaaaaaat aatgaatgtc tgaatgagag tatctcttta
    67021 ttacagaatg ctataaactc caaaaaactt gattttggag tagttataaa aactcattca
    67081 gataatttat tatttgattt tgatacgatt cctgaaaata taatagtagt tgaccatttt
    67141 gaacttacaa tcgaaaaatg gttaagtaaa agtcaacagt tactatagtt tgcaaattat
    67201 ttacagaaag aacaaaaaat gctctgaaac actgatttca aggcattttt tgttagttta
    67261 aaattactcc cattcttctt ttccaaacgt acaatatatc caaaaccatt caaaatacta
    67321 gattctattt tttataatat cactaaatcc acctaattat aggacgtttt cagattttta
    67381 gtcccgtccc agtcccagta ccggagaaat attgttttaa tataatatct ctttttgtct
    67441 tctaagctct taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata
    67501 ttagggcaaa taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat
    67561 agcaaaggtg cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca
    67621 tttttcaaat caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa
    67681 acatggcttg taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc
    67741 aagctaaatt acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa
    67801 atttgctggc agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt
    67861 ttaaaagaag agtttcagct acattcattc tttttctttg agtgtactct gtaatgcttt
    67921 gacaatattt ttccttaaat aaatttttta atttagtacc actcatttta gatatttttt
    67981 caagcgtgcc ttgatttaca ttcgttgcaa aatgatcatc taagaatctt gctacatctt
    68041 caagtgcttt atcatcatca atttcaattt tatatttttt tctatttaag tatgtgtcaa
    68101 ttactatact tatccattca tttgcctttg ctttaaagaa aaaatcagcg gcaggagcgt
    68161 ccatcttaca atttaatatt tccattgcca ctctttctaa ggcctttgta agtattattt
    68221 gattcggttg aagcaaggtt gaataaaaag attctggatt aatgttaata gatgctaaat
    68281 gtttttctat tagctctttt ttaaaaccaa tggaaacagc aagataacaa caattctcgt
    68341 gtaataaaaa aacaaaatca tcttttatat tatcaaaatc aaaagtacat aaagagtttg
    68401 cggtaatagt ttgatacgga ttaaactttt ctccgtttgc actgacaatg taacttgaat
    68461 aaattgaaac atagtctgac atactataag tgctattttg aactacttcc tctttgatat
    68521 aaaaatcatg tatatcgata atgaagctgc ctccttcata aaaccaatat aggccttctg
    68581 cataagttga attctctttt ttccaacaaa atgtatgacc tgcacttgaa tacttctcat
    68641 tgtttttaca ttcatctact ttcatataat cttttacaaa atcataatat gacataacac
    68701 actatccctt ttagacaata ttccaattag ccttattaat tcaaaactat tgtattagta
    68761 attataacag atgtataata gaaaagcaat gatagatatt atcaattaag cgaatttata
    68821 tctaaaaggg atattaaaga aaggagatat gcttatgaag atttacaaaa aactatttgc
    68881 ttatgtccaa gataagaaat atcttggggt tttggccata attttttctg ctatatctgc
    68941 tctacttaca gtatatggat attatttaat ctacaaattt ctagataagt taataattaa
    69001 ttcaaactta tccggtgcag agagtatagc attaaaatct gttattacac taacaagtgg
    69061 agcgatattt tattttgtct caggaatgtt ttcacatatc ttgggattta ggcttgaaac
    69121 aaatttaaga aaaaggggaa tcgatggtct ggaaaaagca agttttaggt tctttgactt
    69181 aaatccatct ggtcaaataa gaaagatcat agatgataat gcagcacaaa ctcatcaggt
    69241 ggtagcacac atgattcccg atagttctca ggcaataatc acacccgtac ttgtacttgc
    69301 acttggcttt atagtaagta taagggttgg cataattttg cttgctctta ctataattgg
    69361 tggcttaatt ttaggggcaa tgatgggcga gcaagaattt atgaagatat accaagaatc
    69421 cctatctaaa ctaagtgctg aaactgttga gtacgtgaga ggaatgcaag ttgtaaaaat
    69481 atttaaagca aatgtagagt cttttaaaag cttttataag gcgataaaag attactcaaa
    69541 gtatgcttat gattattccc tatcttgtaa aaggccttat gttttgtatc aatggttatt
    69601 ttttggactg attgcaattt taattattcc tatagtttat tttatgacta gcttagctag
    69661 cgcaaaggtg attttacttg agcttatcat gattttattt ttatcaggag ttctctttgt
    69721 ttcattcatg agaatgatgt ggtactccat gtatatttct caaggaaatt atgcagtaga
    69781 tactttagag gcgctttacg aagatatgca aaaagacaaa ttagtgcatg gtaatgtcaa
    69841 taattttaaa aactataata tagaatttga gaatgttagc tttgcttata atgataaagc
    69901 tgtcattgaa aatttatcct ttaatttaga agaaggaaag tcctacgcac ttgtcggttc
    69961 atctggatca ggcaaatcaa cagtagcaaa acttatatca ggtttttaca atgttaataa
    70021 aggaagcata aagataggcg ggatagcaat aagtgaatat tctgacgaag ccttaattaa
    70081 agccatttcc tttgtttttc aagattcaaa attattcaag aagagcattt atgataatgt
    70141 agctttagct aataaagatg cgacgaaaga tgacgttatg agagccttaa aattagcagg
    70201 atgcgattta atattagaca aattcccaga aagagaaaat acaatcatag gctcaaaagg
    70261 tgtttattta tccggtggag aaaaacaaag aattgcaatt gctagagcaa ttttaaagga
    70321 ttccaaaatt attattatgg atgaagcatc agcatctatt gacccagata acgagtttga
    70381 attgcaaaaa gcttttaaaa atcttatgaa ggataaaaca gttatcatga ttgcacacag
    70441 gctatctaca attaaagacc ttgatgaaat tattgtcatg gatagtggaa aaattataga
    70501 aagagggtct gacaaagaat taatgtcaaa agatacaagg tataagagcc tgcaagagat
    70561 gtttaacagt gcgaatgaat ggagggtttc aaatgaaaga gttttataaa aaaagatttg
    70621 ctcttacaga tggaggagca agaaatttaa gtaaagcaac actggcttca tttttcgttt
    70681 attgtataaa catgcttcct gccatattac ttatgatttt tgctcaggaa gttttggaaa
    70741 atatgggcaa aagcaatggc ttttatatag tattctcagt tttgactttg atagcaatgt
    70801 atattttgct ttctatcgaa tacgataaat tatataacac aacctatcaa gaaagtgcag
    70861 atttaagaat aaggacagcg gagaatttat caaaattacc tctatcttac ttttctaaac
    70921 atgacatttc cgacatttca caaacaatca tggctgatat tgaaggcata gagcatgcaa
    70981 tgagccactc aataccaaag gtgggcggca tggtactgtt tttcccatta atatctgtaa
    71041 tgatgctagc gggcaatgtc aagatgggtt tagctgtaat tattccatct attttaagct
    71101 ttatatttat acctttatct aaaaaatatc aggttaatgg acagaataga tattatgatg
    71161 tcttaagaaa aaactcagaa agctttcaag aaaatatcga aatgcaaatg gagattaaag
    71221 catataattt atcgaaggat attaaagatg acttatataa aaaaatggaa gatagtgaga
    71281 gagtacactt aaaggcagaa gtaactacaa ttttaacttt gtctatatct tcaatattta
    71341 gctttatatc tcttgctgtt gtgatatttg tcggcgtaaa tctaattatt aataaagaga
    71401 taaattctct ctaccttata ggatatttac tagctgctat gaagataaaa gactctttag
    71461 atgcatctaa agagggcttg atggaaatat tttatttatc gcccaaaata gaaagattaa
    71521 aagaaattca aaatcaagat ttacaagaag gcgatgacta tagcttaaaa aaatttgata
    71581 ttgatctaaa agatgttgag tttgcctaca ataaagacgc aaaagtttta aatggtgtaa
    71641 gttttaaagc taagcaggga gaggtcactg ctttggtagg tgcaagtggc tgcggtaaaa
    71701 caactatctt gaaacttata tcaagacttt atgattatga caagggacaa atcttaatcg
    71761 atggcaaaga tataaaagaa atatcaacag aatctctttt tgataaggtc tctatagttt
    71821 tccaagatgt ggttctcttt aatcaaagca ttatggaaaa tattagaatc ggaaagcaag
    71881 atgcaagtga cgaagaggtt aaaagagcag caaaacttgc aaattgcaca gattttatag
    71941 aaaaaatgga taaaggtttc gatacagtta ttggtgagaa cggagctgag ctatcaggag
    72001 gagaaagaca aagattatca atagccagag ccttcttaaa agatgcgccg atattgatct
    72061 tagatgagat aacagcaagc cttgatgtta acaacgagaa aaagattcaa gagtctttaa
    72121 ataatttagt taaagataaa actgttgtaa tcatttcaca tagaatgaaa tccatagaaa
    72181 atgcagacaa gatagtagtt cttcaaaacg gaagagtaga aagcgaaggt aagcatgaag
    72241 agcttttaca aaaatcaaaa atttacaaaa atttaataga aaagacaaaa atggcagaag
    72301 aatttattta ttaggaggac tacaatggat aataaaaaat taaaagtaaa agatttagta
    72361 agtatcggtg tttttggcgt aatttatttt gccttcatgt ttggagttgg tatgatgggc
    72421 ttgattccaa tattgttctt aatatacccg acagtattag ccatagttgc aggaactgtt
    72481 gttatgttat ttatggctaa ggttcaaaag ccatgggcac tatttatatt tggtatgata
    72541 tcaccacttg tgatgtttgc agctggtcat acctacgtag ttgtggtttt atcacttata
    72601 gtaatgataa tagcagaatt aattagaaag attggtaatt ataattcatt taaatacaat
    72661 atgctttctt atgcaatctt cagcacatgg atatgtagct ctttaatgca aatgctttta
    72721 gcaaaagaaa aatatatgga gtggtctttg atgacgatgg gaaaagatta tgttgatgta
    72781 ttagaaaagt taataactta tcctcacatg gctttagtag ccttaggtgc tttcttagga
    72841 ggaattcttg gagcatatat aggcaaggct ctattgaaaa aacactttga aaaagcaggc
    72901 attgtataat gtctactttt tcttcaaatg ctccctttaa tcccaatccg atctctaaga
    72961 tattagtcgt atttatcaca ggcttaactg ttatgcatag cataaacatc cgttttgagc
    73021 tggcaattgt ttgtcttatt ggtattttgc tttatttgaa tggatacaaa aaaacacttt
    73081 tcaaatggat tatattgtgt gggatacttt actccttgcc taattttatg gtgttatctg
    73141 aattaaaccc tatagttaag atgtttttga gtatacctat tgttattaga atgtttattt
    73201 taccatttat ggcagcaagc tttatgataa agacctcgga tgtaggcgca ataatttcat
    73261 cgatggataa gcttaagatt tcaaagaatg tatccatacc tattgcggtt atgtttagat
    73321 tcttcccatc ttttaaggag gagaagaaaa acatcaaaat ggctatgaga gtaagaggga
    73381 taagttttaa aaacccagtc aaatatcttg aatatgtttc tatgccacta ctcattatat
    73441 catctaatat atcagatgac attgcaaaag cggcagaaac aaaggcaata gaaaatccaa
    73501 ttgccaagac cagatacatt cgcgtaaaga tacagctaat tgattttgtt tatgttttag
    73561 cggttgctgg acttattgtg ggaggcttaa tatggttgaa ataaaaaatt taagtcttga
    73621 ttatggtgaa gagcatatat tagatgatat atcactatcc atagccgagg gagagtgcgt
    73681 gctatttaca ggaaaaagtg gaaatggtaa gtcatcttta ataaattcaa tcaatggact
    73741 agctgtaagg tatgataacg caaagacaaa gggcgaaata attattgatg gtaagaatat
    73801 aaaaaatttg gaactttatc aaatctcaat gcttgtttca actgtttttc aaaatcctaa
    73861 gacatatttt tttaatgtca atacgacatt agaattatta ttttatttgg aaaatatcgg
    73921 tcttgcaaga gaagagatgg acaggcgttt gaaggatatg cttaagatat tcccgataaa
    73981 aaatcttttg aacagaaata tatttaatct atccggcggt gaaaaacaaa ttctttgcat
    74041 tgcagcttct tatatagcag gtacaaagat tatagttatg gatgagcctt catcgaattt
    74101 agatattaaa agcataaatg ttttggcaaa gatgctaaag atattaaaag agaaaggcat
    74161 aagcataatt gttgcagagc atagaattta ttatttgatg gacatagttg accgtgtatt
    74221 tttaatagat aaaggaaagc ttaaaaaaac ttatactaga agtgaatttt taaagctaga
    74281 taaaaatgaa ttaaatgctt taagtttaag agataaagaa ttaagtaaat taaaagttcc
    74341 ttatttaaaa gaaggtggag agtatcagat aaaaaatctt agctacaaat ttactgatga
    74401 tgagtgttta agcttaaaag atatttcatt caagcttggg aaaatttatg gcataatagg
    74461 atccaacgga cgaggaaaat caacgctttt aagatgttta ataggtcttg agaaaaaatc
    74521 aaaagaagaa atttatttta agggagagaa gctatctaaa aaagaaagac tcaaaaactc
    74581 ttcacttgtt atgcaagatg taaatcatca attattcaca gatgaagtat tcaacgagct
    74641 tagattagga gtaaagaatt ttgatgaaga aaaggcgaaa atcattttaa aagatttagg
    74701 cctggacgaa tttattgaaa ggcatccgat gagtttatca ggagggcaaa agcaaaggct
    74761 tgcaatagca tctgttatgt gcaagaattc tccatttatc ttttttgacg aaccttcaag
    74821 tggtatggat tattccaata tgataaaaat atctgaactg attaataagt ataaaaccat
    74881 ggataaaata atttttattg tttcccatga tatagaattt ttaaatgaag tggcagatga
    74941 aatttttgaa ttgtaaaata gagtctgtta aacatgtcta tagaactagt aaattgtcta
    75001 aaaatttaaa agatactaac acatattgat atttatagaa aattaacttc aggattttta
    75061 cagtatttat aaactttata atatgtattt atagtattaa taatttccat atctttaggg
    75121 aaattattta tttacttacc ataattaaag agatgagaga taaatatcgg c
//
LOCUS       DBERYS010000006        63038 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.006, whole genome shotgun sequence.
ACCESSION   DBERYS010000006 DBERYS010000000
VERSION     DBERYS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 63038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.006"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            149..871
                     /locus_tag="ACL8LA_000445"
     CDS             149..871
                     /locus_tag="ACL8LA_000445"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1812262.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            1088..2422
                     /gene="pepC"
                     /locus_tag="ACL8LA_000446"
     CDS             1088..2422
                     /gene="pepC"
                     /locus_tag="ACL8LA_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGS1812263.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(2478..3389)
                     /locus_tag="ACL8LA_000447"
     CDS             complement(2478..3389)
                     /locus_tag="ACL8LA_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1812264.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(3413..4216)
                     /locus_tag="ACL8LA_000448"
     CDS             complement(3413..4216)
                     /locus_tag="ACL8LA_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGS1812265.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(4244..5233)
                     /locus_tag="ACL8LA_000449"
     CDS             complement(4244..5233)
                     /locus_tag="ACL8LA_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1812266.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(5466..6485)
                     /gene="adhP"
                     /locus_tag="ACL8LA_000450"
     CDS             complement(5466..6485)
                     /gene="adhP"
                     /locus_tag="ACL8LA_000450"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGS1812267.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(6837..7649)
                     /locus_tag="ACL8LA_000451"
     CDS             complement(6837..7649)
                     /locus_tag="ACL8LA_000451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1812268.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            7839..9257
                     /locus_tag="ACL8LA_000452"
     CDS             7839..9257
                     /locus_tag="ACL8LA_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGS1812269.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            9310..10017
                     /locus_tag="ACL8LA_000453"
     CDS             9310..10017
                     /locus_tag="ACL8LA_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1812270.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            10112..11086
                     /gene="folP"
                     /locus_tag="ACL8LA_000454"
     CDS             10112..11086
                     /gene="folP"
                     /locus_tag="ACL8LA_000454"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGS1812271.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALATGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            11088..12410
                     /locus_tag="ACL8LA_000455"
     CDS             11088..12410
                     /locus_tag="ACL8LA_000455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS1812272.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            12391..12945
                     /gene="folE"
                     /locus_tag="ACL8LA_000456"
     CDS             12391..12945
                     /gene="folE"
                     /locus_tag="ACL8LA_000456"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGS1812273.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            12988..13800
                     /gene="folK"
                     /locus_tag="ACL8LA_000457"
     CDS             12988..13800
                     /gene="folK"
                     /locus_tag="ACL8LA_000457"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGS1812274.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(13845..14162)
                     /locus_tag="ACL8LA_000458"
     CDS             complement(13845..14162)
                     /locus_tag="ACL8LA_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGS1812275.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            14519..14965
                     /gene="rplM"
                     /locus_tag="ACL8LA_000459"
     CDS             14519..14965
                     /gene="rplM"
                     /locus_tag="ACL8LA_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGS1812276.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            14984..15376
                     /gene="rpsI"
                     /locus_tag="ACL8LA_000460"
     CDS             14984..15376
                     /gene="rpsI"
                     /locus_tag="ACL8LA_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGS1812277.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(15638..16060)
                     /locus_tag="ACL8LA_000461"
     CDS             complement(15638..16060)
                     /locus_tag="ACL8LA_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1812278.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            16207..16383
                     /locus_tag="ACL8LA_000462"
     CDS             16207..16383
                     /locus_tag="ACL8LA_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812279.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            16785..18998
                     /locus_tag="ACL8LA_000463"
     CDS             16785..18998
                     /locus_tag="ACL8LA_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGS1812280.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(19220..19696)
                     /locus_tag="ACL8LA_000464"
     CDS             complement(19220..19696)
                     /locus_tag="ACL8LA_000464"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGS1812281.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQAQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            19919..23122
                     /locus_tag="ACL8LA_000465"
     CDS             19919..23122
                     /locus_tag="ACL8LA_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGS1812282.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKGG
                     AITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYYWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            23502..23878
                     /gene="tnpA"
                     /locus_tag="ACL8LA_000466"
                     /pseudo
     CDS             23502..23878
                     /gene="tnpA"
                     /locus_tag="ACL8LA_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            24060..24122
                     /locus_tag="ACL8LA_000467"
                     /pseudo
     CDS             24060..24122
                     /locus_tag="ACL8LA_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(24266..24895)
                     /locus_tag="ACL8LA_000468"
     CDS             complement(24266..24895)
                     /locus_tag="ACL8LA_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGS1812283.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(24905..25906)
                     /locus_tag="ACL8LA_000469"
     CDS             complement(24905..25906)
                     /locus_tag="ACL8LA_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGS1812284.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(25937..26578)
                     /locus_tag="ACL8LA_000470"
     CDS             complement(25937..26578)
                     /locus_tag="ACL8LA_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGS1812285.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(26597..27412)
                     /locus_tag="ACL8LA_000471"
     CDS             complement(26597..27412)
                     /locus_tag="ACL8LA_000471"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGS1812286.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            27683..28117
                     /locus_tag="ACL8LA_000472"
     CDS             27683..28117
                     /locus_tag="ACL8LA_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1812287.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQESVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            28129..29319
                     /locus_tag="ACL8LA_000473"
     CDS             28129..29319
                     /locus_tag="ACL8LA_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGS1812288.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            29330..29821
                     /locus_tag="ACL8LA_000474"
     CDS             29330..29821
                     /locus_tag="ACL8LA_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1812289.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            29836..30615
                     /locus_tag="ACL8LA_000475"
     CDS             29836..30615
                     /locus_tag="ACL8LA_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1812290.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            30602..31420
                     /locus_tag="ACL8LA_000476"
     CDS             30602..31420
                     /locus_tag="ACL8LA_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1812291.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            31420..31713
                     /gene="yajC"
                     /locus_tag="ACL8LA_000477"
     CDS             31420..31713
                     /gene="yajC"
                     /locus_tag="ACL8LA_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS1812292.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            31735..33636
                     /locus_tag="ACL8LA_000478"
     CDS             31735..33636
                     /locus_tag="ACL8LA_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGS1812293.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            33696..34697
                     /locus_tag="ACL8LA_000479"
     CDS             33696..34697
                     /locus_tag="ACL8LA_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1812294.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(34807..35142)
                     /locus_tag="ACL8LA_000480"
     CDS             complement(34807..35142)
                     /locus_tag="ACL8LA_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812295.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(35153..35395)
                     /locus_tag="ACL8LA_000481"
     CDS             complement(35153..35395)
                     /locus_tag="ACL8LA_000481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812296.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(35411..35605)
                     /locus_tag="ACL8LA_000482"
     CDS             complement(35411..35605)
                     /locus_tag="ACL8LA_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1812297.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            35771..36721
                     /gene="rsmH"
                     /locus_tag="ACL8LA_000483"
     CDS             35771..36721
                     /gene="rsmH"
                     /locus_tag="ACL8LA_000483"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGS1812298.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            36733..37050
                     /gene="ftsL"
                     /locus_tag="ACL8LA_000484"
     CDS             36733..37050
                     /gene="ftsL"
                     /locus_tag="ACL8LA_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGS1812299.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            37054..39306
                     /gene="pbp2X"
                     /locus_tag="ACL8LA_000485"
                     /gene_synonym="pbpX"
     CDS             37054..39306
                     /gene="pbp2X"
                     /locus_tag="ACL8LA_000485"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGS1812300.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            39308..40288
                     /gene="mraY"
                     /locus_tag="ACL8LA_000486"
     CDS             39308..40288
                     /gene="mraY"
                     /locus_tag="ACL8LA_000486"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGS1812301.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            40852..42957
                     /locus_tag="ACL8LA_000487"
     CDS             40852..42957
                     /locus_tag="ACL8LA_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGS1812302.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(43253..43735)
                     /locus_tag="ACL8LA_000488"
     CDS             complement(43253..43735)
                     /locus_tag="ACL8LA_000488"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGS1812303.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(43830..45314)
                     /locus_tag="ACL8LA_000489"
     CDS             complement(43830..45314)
                     /locus_tag="ACL8LA_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGS1812304.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDATRAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            45467..47077
                     /locus_tag="ACL8LA_000490"
     CDS             45467..47077
                     /locus_tag="ACL8LA_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGS1812305.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(47221..48617)
                     /locus_tag="ACL8LA_000491"
                     /pseudo
     CDS             complement(47221..48617)
                     /locus_tag="ACL8LA_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            48795..50240
                     /gene="cps4A"
                     /locus_tag="ACL8LA_000492"
     CDS             48795..50240
                     /gene="cps4A"
                     /locus_tag="ACL8LA_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGS1812306.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHRKFLFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTDRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            50242..50973
                     /gene="cps4B"
                     /locus_tag="ACL8LA_000493"
     CDS             50242..50973
                     /gene="cps4B"
                     /locus_tag="ACL8LA_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGS1812307.1"
                     /translation="MIDIHSHIVFDVDDGPKSIEESKALLREAYNQGVRMIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYREIHAGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVLKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKEFFVDNPRKIIMDQLI"
     gene            50982..51674
                     /gene="cpsC"
                     /locus_tag="ACL8LA_000494"
     CDS             50982..51674
                     /gene="cpsC"
                     /locus_tag="ACL8LA_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HGS1812308.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            51684..52367
                     /gene="cpsD"
                     /locus_tag="ACL8LA_000495"
     CDS             51684..52367
                     /gene="cpsD"
                     /locus_tag="ACL8LA_000495"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HGS1812309.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARTGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTAIGEVNKRDVQKAKQQLEQTEKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
     gene            52383..53750
                     /locus_tag="ACL8LA_000496"
     CDS             52383..53750
                     /locus_tag="ACL8LA_000496"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1812310.1"
                     /translation="MDEKGLKIFLAVIQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMG
                     IDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLIL
                     FAIASLVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTM
                     QGGMFKIDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            53756..54499
                     /locus_tag="ACL8LA_000497"
     CDS             53756..54499
                     /locus_tag="ACL8LA_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HGS1812311.1"
                     /translation="MNERMQILGVTIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCHEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEILQDMLKVFKQRYPNLNVVGYRNGYFSPEDEKKIQEDIKGGKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQKLNLEWLFRVVN
                     EPKRLFKRYFIGNVTFIKRVLDEKRKSKK"
     gene            54474..55559
                     /locus_tag="ACL8LA_000498"
     CDS             54474..55559
                     /locus_tag="ACL8LA_000498"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1812312.1"
                     /translation="MKSENQKNNILHISRTMDIGGAERIVYQLSSDLKDEFDSVHVAS
                     TGGLWESKLLSKGVQHHKILDVDSKNPLTMVKILSSFYRIIKNNDITIVHTHHRMAAF
                     YIRLLKIINPKLIHVYTAHNVFKNKLPLYKFSLKKAYCVAVSKAVNDNLKHDVGISDS
                     TVIYNGVVFEKTDKLVGEIVNYNGIKLGCIARLSEQKGLTYLIDAMSLVKHRNITLFI
                     VGDGELRDELENKVKQLNLQKSVIFLGYRKDIVECINSFDYLVSSSLYEGFGLNAIEA
                     FMKGKTMVSSDIPGINEVVNNKNGILVPVKDDVALARAIEKLATDKKLREKLAYQAKK
                     DYETKFNYSLFLDNYRRLYRKLMGESK"
     gene            55556..56644
                     /gene="wecB"
                     /locus_tag="ACL8LA_000499"
     CDS             55556..56644
                     /gene="wecB"
                     /locus_tag="ACL8LA_000499"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGS1812313.1"
                     /translation="MKKVMLVFGTRPEAIKMCPLVNELKQNDSIKTLVCVTGQHKEML
                     EQVLEVFKVVPDYDLGIMKANQTLFTITTSILDKIQAVLEKEKPDLVLVHGDTTTTFA
                     TALAAFYMGIKVGHVEAGLRTYNLQSPFPEEFNRQTTSIIADYHFAPTEVAKENLLRE
                     DRKNIFVTGNTVIDALKTTVQDNYHHPILEWAKGSKLIMLTAHRRENLGKPMENMFNA
                     VNRILNEFEDVKVVYPIHKNPKVRELASKVFGDNERMKIIEPLEVIDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVVAGTLKLVGTEEENIYSNFKLLLEDET
                     EYNKMSKASNPYGDGMTCKRIVDIILEV"
     gene            56761..57825
                     /locus_tag="ACL8LA_000500"
     CDS             56761..57825
                     /locus_tag="ACL8LA_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS1812314.1"
                     /translation="MATLFALLSTLVKLFVTFDYIPKVLLQHKVTLIDILVVLFLFFQ
                     AFAAFQSQTLYLNYVGGQFFLLGLYSFLKHFLLLDCKTTIKSLFLTFILFLCVQVITQ
                     LLFPVGFDSLHPTGDNRLYFLGRKNIATPYIIVGLGGFYLLNKKMNEFISLKEIIFLG
                     LFGILSFLTQSSTAIICYGLFIFIRLLGLKENIGKLYSLVSMAVYVCFSLSIIFSQST
                     ILSTFTAIFSKNATFSGRINIWQLAIRIFEENFWFGRGLNVNFNAWTNGIIVNSAHNT
                     LLDILARTGIFPGVLFIVLLMNLFLGKYRIESKTLLAMLASFMIYITMETSSMSILLL
                     IIVLCVYWPRGEEKLHEQVT"
     gene            57809..58678
                     /locus_tag="ACL8LA_000501"
     CDS             57809..58678
                     /locus_tag="ACL8LA_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGS1812315.1"
                     /translation="MSKLLNTIYYRLFRLYKKIYLQVFSKKTVDILGEKANLENELIV
                     SFTTIPSRLNYLPSMIKSIFNQTIIPNRFIMYVYKDEFEGINLESILELEIKNGLEIV
                     YVDENLRSHKKYFYAMKDNPNSIVVLVDDDIIYPRNTIKKLIASYRIYPQCVSAMRCH
                     RIKLFSDGSLYPYNQWEYEISGATIPSYFNFFTSGGGTLFPPCTRNEDLFNKKNIREL
                     SFLADDVWLNFLVVKNGIKTVKATRYKGTPLTIDDNPEESLVYLNTVYDNNNDKCIRN
                     MVEYYQINFTEDK"
     gene            58678..60102
                     /locus_tag="ACL8LA_000502"
     CDS             58678..60102
                     /locus_tag="ACL8LA_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGS1812316.1"
                     /translation="MNVEKNLKRGIFYTAIGKYSNVVIQLLVTAILSRILSPVEYGIV
                     AVVNVFLLFFQMLADSGIGPAIVQNKELNKSDLISIFSLTIYSGIVLSCIFALLGYPM
                     GVLYGETIYVSLFPLLGLCVLFYTITIVPQAILMKTMNFKIVNFLTIFSNIASGLVGV
                     ILAVSHFGVYSLIFSNIVKAMVLFIALYSKAKINFYWKVSKSSIGKIFEFSKFQFLFN
                     FWNYFARNLDNLLIGRLISPSDLGYYDKAYQLSLYPNQILSQVVTPALHPIMSNFQDN
                     ISKIGEVYLQISRIFVIVGIPISAYLYFNAQYVVTFMFGDNWSQSVPVFQILAATIWL
                     QMANSPTGAFYQATNQTKLLFRIGLLTSFINILAIVIGVMLQSIQCVAYMLLISFSLS
                     LVINTLYLTKKVLNISAQKYIKPILINLTIISPYIVFNLFISDFVNDLILHLSVQFVI
                     LFLIWGIGMYISGEYRRVFAVIRK"
     gene            60115..61317
                     /locus_tag="ACL8LA_000503"
     CDS             60115..61317
                     /locus_tag="ACL8LA_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1812317.1"
                     /translation="MKILFVNMFYNEAGGAQKSTKFLAEQLVKEGHEVFAYSDDAIDS
                     DIDEVINRVKIFRRRTPLFSLHYIFSAKKNPAKHFFYKIFETYNVFAKRKFINIIEEV
                     QPDIVHFNTISGMSLSIVKEAKKRNIRTVWTLRDYWLEYPWGEKDISLIKLLNRIYRP
                     FVKKSLRHLDIVTAPSAFTLNNFINKGFINPSSQKCMVIENAVIFSKGKLENIIKQKQ
                     ETKRNPEKTNFLFVGSILEIKGVFNLVKAFKKLTSPEISLKIVGKGKDLEKLQKEIES
                     DPRIQYLGFQDTETLYKTYQNSDVLVVPSAWDEPFGRVVIEGNANGLPVITSDRGGLP
                     EIVQTVGGGEIFTDESGNTLYSLLKKFVEMENYNVYYQCILENIDIYSIEKQSEKFTN
                     LYLDKEIE"
     gene            61351..62583
                     /locus_tag="ACL8LA_000504"
     CDS             61351..62583
                     /locus_tag="ACL8LA_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGS1812318.1"
                     /translation="MKIAVAGTGYVGLSIAVLLAQHHEIKAVDIVAEKVELINQKKSP
                     IQDDYIEKYLVEKDLNLEATMDAKYAYEDAEFVVVAAPTNYDSKKNFFDTSAVEAVIQ
                     QVKKINPKAVIIIKSTIPVGFTESVQKKYQYKNILFSPEFLRESKALYDNLYPSRIIV
                     GTDLEDTYLKDKANIFASLLKEGAIKEDIDTLFMGVTEAEAVKLFANTYLALRVSYFN
                     ELDTYAEVKGLDAKAIIDGIGLDPRIGNHYNNPSFGYGGYCLPKDSKQLLANYFDVPQ
                     NMMTATVESNRTRKDFIAQRILEKAGVSETDSLDAFKNIQDIVIGIYRLTMKSNSDNF
                     RHSSIQGVMKRLKAKGVTVIIYEPTLKDGETFFGNKVVNNLDKFKEASNVIVANRFEP
                     SLEDVSNKVYSRDIFKRD"
     gene            62802..62981
                     /locus_tag="ACL8LA_000505"
                     /pseudo
     CDS             62802..62981
                     /locus_tag="ACL8LA_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        19395 a        10903 c        13644 g        19096 t
ORIGIN      
        1 tttgaggttg cagatagaac tgacgaagtc agtaacatat atacggcaag gcgacgttga
       61 cgcggtttga agagattttt gaagagtatg aaacgaaaag gaggttggtc attgtactag
      121 cctccttttg atatgatata atagttctat gagattagat aaatttttag ttgcctgtgc
      181 ggtgggaagt cggactgagg tcaaaaactt gctcaaggct gggcgcgtga ctgtaaatgg
      241 taaaaaagaa aaatcagcta aattgcagat tgatgaaaaa atagatgaga ttcgctttga
      301 tgggcaagtg ttggagtatg aagagtttgt ctactacatg atgaacaagc ccaaaggagt
      361 tatctcagcg actgaggatc ccaagcacag aaccgttctg gacttgttgg atgacttggc
      421 gcggagtaag gaagtttttc cagtaggacg cttggatatt gacacgcatg gtcttttgct
      481 cttgaccaat aatggtcagc tggctcatgt tcttctttcg cccaagcgtc atgtggacaa
      541 gacttatcag gctcaggtca agggaattat gacccaagaa gatgtggaga catttatcaa
      601 gggcattccg ctcaaagact ttacctgtca gcccgctaga ctggagattc tttccacaga
      661 tgcagaaaag aatcagagcc aaatccgtgt gaccattgca gaagggaagt ttcatcagat
      721 caagcgtatg gtgggctact gtggcaagga agtggtggac ttgcaacgat tgactatggg
      781 aactttagta ttagatgaga acctggaacg aggggaatgg cgtcgcttga ccaaggaaga
      841 attagaaatt ctccgcgcta atattattta atttagttta atactcaatg aaaatcaaag
      901 agcaaactag gaaactagcc gcaggctgta cttgagtacg gcaaggcgac gttgacgtgg
      961 tttgaagaga ttttcgaaga gtataaattc gaaaaaggtg agggagaatg tccttacctt
     1021 ttatgttttt tagttgcttc ctttgtgaaa agagttataa tagactgtag aataaaagga
     1081 ggaatctatg aacgcgattc aagaatcatt tactgataaa ctatttgcca actatgaagc
     1141 aaatgtaaaa taccaagcga ttgaaaatgc tgccagccac aacggaattt ttgcagctct
     1201 ggaacgtcgc caaagccatg tagacaacac acctgttttc tcattggatt taaccaagga
     1261 caaggtcact aaccagaaag cgtctggtcg ttgctggatg tttgcggctc tcaacacctt
     1321 ccgccacaaa ctcatctcgc aatacaaatt ggagaacttt gagttgtcac aagcccacac
     1381 tttcttctgg gataagtatg agaaatcaaa ctggttcttg gagcaagtca ttgcgacttc
     1441 agaccaagaa ttgactagcc gcaaggttag cttcttactc caaacacctc aacaagatgg
     1501 tggtcaatgg gatatggtcg tttccctctt tgaaaaatac ggtgtcgtgc ctaagtcagt
     1561 ttatcctgag tctgtttcat ctagcagcag tcgtgagcta aatgcgatcc ttaataaatt
     1621 gcttcgtcaa gatgctcaaa tcttgcgtga tttgcttgtt tctggtgcag atcaagtgac
     1681 tgttcaagct aagaaagaag acctcttgca agaaatcttt aactttcttg ctatgtcatt
     1741 aggacttcca ccacgcaagt ttgactttgc ttatcgcgat aaagataaca actacaaaag
     1801 tgaaaaagga atcacaccac aagagttcta caagaaatat gtcaatcttc ctttagaaga
     1861 ctacgtttct gttatcaatg ctccaactgc tgataaacct tacggaaaat cttacacagt
     1921 tgagatgttg gggaatgtgg ttggtagccg tgcagttcgt tacatcaacg ttccaatgga
     1981 gcgcttgaaa gaattggcga ttgcccaaat gcaagcaggt gagactgttt ggtttggttc
     2041 tgatgtcggc cagctcagta accgtaaggc tggaatcctt gcgacagatg tttatgactt
     2101 tgaatcaagc atggacatta aacttactca agacaaggct ggacgtttgg actatagcga
     2161 aagcttgatg acccacgcca tggtcttgac aggtgttgac ttggacgaaa atggtaaatc
     2221 aaccaagtgg aaggttgaaa actcatgggg agacaaggtc ggtacagatg gttactttgt
     2281 tgcctcagac gcttggatgg acgaatacac ataccaaatc gttgttcgta aggaattgct
     2341 gacagcagaa gaacaagctg cctatggagc agaaccaatt gtacttgcac catgggatcc
     2401 aatgggagcc ttggctaaat aaaagcatag aaaaaaggaa tcagatttta gaacctggtt
     2461 cctttttagt tgcttgatta catgatgtga agaacatgtg ccacaatacc cactgcgaag
     2521 agggcaagga taatagtgat tggagatact tttttcttaa gtaagtacat gcaaagtaaa
     2581 gtaaggagta gtcctgataa tccaggaatc aacatatcca agttttgttg gaaagtagta
     2641 actttttcag gagtttgaga caatccttgt cctacttgtg cgaatgcttc ttggatacct
     2701 ttagaccctt ctggcaattt atcccaatgg atataagcct tttcatctag ttgaacttta
     2761 gaaacatcga aagcaaattt aatatttacc cagcgttgaa caaggacagc aagaatgaac
     2821 atcccaagga tagaagctcc tttagtgata tcttgaagga taccaccaga catatcttta
     2881 gtgatttctg atccagcctt gtatccaatc tcttgaacat accacaagaa tgacatacga
     2941 atcaagttcc atgcaacaaa gaagaggagt ggccccaaga tattgccagt aagggcaagt
     3001 gaagcaccga gagatccaag gattgggcgt actgtaaacc agaatactgg gtcaccgata
     3061 ccagcaagag gtcccatcat accgatttta accccttgga tagcagcgtc atcgatttcc
     3121 acaccgttag cacgttcttc ttcaagcgca agagtaaccc ccatgactgg agcagctacg
     3181 tatggatgag tgttgaagaa ctcaaggtga cgctcaagag cagcgatttg atcttctttt
     3241 ttagtataga gttttttgat agctggaatg agtgtataag cccagcccaa gttttgcatc
     3301 cgttcaaagt tccaagaccc ttgtaagaag gttgaacgcc accaaacttt tttacgatct
     3361 gatttagtta attgaagttt ttcagtcatg atgttttcag tcctttctta tcttagtagt
     3421 cttctaggat atcgccgatt gggtcgttag aagttgcggc tcctccgcca ccatttccac
     3481 cagttttaga aaggtgaagg tagataagag cgatagcaac accgatagca ccgaatccga
     3541 ttagagtaat atctgacaca gcagcgagaa cgaaaccaag agcgaagaat ggccatactt
     3601 cacgagttgc catcatgttg ataaccatgg cgtaaccaac ggcaacgacc ataccaccac
     3661 cgatagccat accatctttg agccagtctg gcatggcact aaggatactt tgtacagttt
     3721 cagttggtac cataaggaga agagctgcag gaagcgcgat acgaagtcct tggaagagta
     3781 gcgcaatgaa atgcgcacgc tccacagcgc cgaagtcacc ttttttagcg gcagcatctg
     3841 cagtatgaac caaaccaact gaaattgtac gaacaatcat tgtcaagaaa agtccagcta
     3901 cggcaagagg gatagcaacc gcttgggcaa caccgatacc agtcttggta aagtcaccac
     3961 caagaaccat gataatggca gcagcgacag aagcaagtgc agcatcagga gcgatagcag
     4021 caccgatatt tgaccaacca agggcaatca tttgaagcga tccaccgagg ataatccctg
     4081 cttccaagtg acctgttaca agcccaataa gggtacaggc tacaagtggt tggtgaaatt
     4141 ggaactggtc gaggatgcct tcaagacctg caaagaaggc tacaacgact actaaaacca
     4201 tagaaataat agacatgttt aaaatccttt cataaataat ggcttatttg acattggctt
     4261 tgttaatcaa gtcaaacaaa tcttttttag aatcatttgg tactttacgg acatcaaatt
     4321 caacacccaa gtcacgcatt ttttcaaatg tagcaacatc ttctttgtcc atagacaaaa
     4381 cggtattgac caatgtttta cctgttgagt gagccataga accaacgtta agagtcttga
     4441 ttggcacgcc gccttcgatg gcacgaaggg catcttgagg tgtttcaaac aagataaggg
     4501 catgtgtttc tccaaaacgt gggtcttttg aaatctcaat cagtttttga attggaacca
     4561 cgttagcctt gacattacct ggagctgctt gtttaatcaa ttctttacga aggtcgtctt
     4621 tagccacgtt atctgaagca acgatgatac gatttgcttt tgaatctgga gtccaagcag
     4681 ttgcaacctg accgtgaagt agacgtgtgt caagacgggc aagattgatt ttcaatttac
     4741 cgtctccgat aacagttcct tctgggatag cagtttgggc aactggagca gctgcagcgc
     4801 ttgcaacttc ttcaactgga tttagctctt ctggaagagc tttgatgcca tctttggctt
     4861 ctttaatgat attagcagcg actttttcta cacctgcagc agcgtccatg aggcgctctg
     4921 tgtaggcttg aatcaacatc ggtaagttaa gtcctgtgat gatggcaaac ttacgctcag
     4981 gattttctcc catcacgcga ctagcttggt taaatggaga accactccaa aggtcagcca
     5041 aaactagaac ctcatcttct gcgtcaaatg cagcaacagc gttattaaac ttagcgtata
     5101 gatcatcagg accttcattt ggcataaagg tcacaacttg aaccttttct tgttcaccaa
     5161 agatcataga tcctgactga tgaatacccg cagcaaattc gccgtggctc gcaataatga
     5221 ttccgatact cattattgtc attcctcctt ttttgtttta gttttcattt aagaaaactt
     5281 taagactgtt taagtataaa ccgttttcat aaaaaaagca actatttatc ataaaaaatt
     5341 aaaaaatttt tatttttgta tgttgatatg ttgagttttt gaggagtaat cttgaattct
     5401 tatttaaaat ttaatataaa aaagttggaa gaagcttcca acttttaaaa taggtttcta
     5461 ttagattagt gggtgaagtc gagtaccata cgtccttgga tttggccttt ttccatttcg
     5521 tcgaaaatgg caacagcatc ttctactgga cgtttttgaa caactgggac taccagacct
     5581 tctgcaccaa attggaaggc ttcttctaag tctttacgag ttccgacaag agaaccgatg
     5641 acttggattc catcgaggac tgttttaacg atgctgagtt ccatcatttc agaaggaaga
     5701 ccaacagcga cgacgcgacc accagcacga atggagtcaa cagcctggtt gaaggcaact
     5761 ttagacacag cagttacgac agctgaatga gctcctccat cagttttttc tttaatgagt
     5821 ccagctacat cttcaacttc gaggccgtta atcacaatgt cagcgcctac ttcttttgca
     5881 agggcaagtt tgtcattgtt gatatcgact gcgataacat gagcattgaa tactttttta
     5941 gcgtattgaa cagcgaggtt accaagtcca ccagcaccgt aaagaacagc ccattggcct
     6001 ggttcaactt ttgcttcttt gatagcttta taggttgtta ctccagcaca tgtgatagaa
     6061 gaagcttggg ctggatcaag tccgtcagga actttgacag catagtcagc agttacgata
     6121 cattgttcag ccataccacc gtctactgag tagccagcat ttttcactgt acggcaaagg
     6181 gtttcgcgac cagttgtaca gtattcgcaa gtgccacatc cttcaaagaa ccaagcaacg
     6241 ctgacgcggt caccgacttt aaggcttttc acatctggag caatctcttt aacgataccg
     6301 ataccttcgt gcccaagaac acgtcctggg acttgaccaa agtcaccatg agcaacgtgg
     6361 aggtcggtgt ggcaaacgcc acagtattca acttctacaa gtgcttcccc agtttcaagt
     6421 ggacggagta ctttttcttc aatagcaaca ccagtgcttt ctggatttac aacaacagct
     6481 ttcatagcta tcctccttga tattgaccga gaacagggac aacgggactg gattgatttt
     6541 ttgtcaacag agtcgagtgt gacgagctct cttgtattta tatgtgaagt atatcacaaa
     6601 agaaagcctt ttccaaggtt ttggagacaa aaagtagaac aatcgattaa ctggttgatg
     6661 agactgtgaa aagagcacaa agaccagctt gcaagctggt catactcttc gaaaatctct
     6721 tcaaaccacg tcagcttcat ctacaacctc aaagcagtgc tttgagcaac ctacggctag
     6781 cttcctagtt tgctctttga ttctcattga gtatcagtat aggttataag aataagttat
     6841 aagaataagt cttgtagggt tttggcaacc ccatcttggt cgttggtaag ggaaatttgc
     6901 tcatctgcat aagggagtag ctctggattg gcatttttca tggcataacc cttcccagca
     6961 aaagcgagca tttcggtatc attgtgttca tctccaaagg caatcaaatc ttttttgtca
     7021 cgattcatta tcttgagcaa gtagtccaaa gcaaaggcct tgttgacacc ttttggggta
     7081 cattcaagga tattgagcgg acctccccag gtattgatag aaagttgatg ctggtagaag
     7141 gcgttcattt cttttgccaa ggcatatttg tcactggctc tggtctgcaa gaggatacag
     7201 ttagggtcct tggtcaccaa ttcaggctgg aattgatctt caggctggaa agcttctaca
     7261 ccaaatagtt tgggattggc aatttcttca ttgggatttg taatgtagaa ttttttacga
     7321 tattctccag cgataaaatc ggcttgaatg tcctctgaac gttgaaccat atctagcaga
     7381 tattttttgt ctacagtcaa acacttttca aaatcccaaa cttggtctgg taaatgagta
     7441 agggatccgt tgaagttaat cataggagtg tctaagccca gttcacggta aaaatctttt
     7501 gacatacggt aagggcgacc tgtcgtaata ataacctgat ggcctttttc agcaactttt
     7561 ttgatggttt ttttggtaaa gtcagaaatt tgactgtctg agttgagcag ggttccatct
     7621 aggtcaactg caataatttt tttcgtcata tggacctttc tagtgtgttt tcagataaga
     7681 tgtcttctat tataacagaa agcaggcaga aaagcagatt ccaaacaaaa aatgaaattg
     7741 gattaatttc ttaaacaaat caaacaaaga cattgaaaaa gtgaatgata aatgatataa
     7801 ttctattatt gttcgtaaaa attaaaagga gattgatgat ggacaaatta tttaaactaa
     7861 aagagaacgg tacagacgtt cgtacagagg ttctcgctgg tttaacaact ttctttgcaa
     7921 tgagctatat tctctttgta aacccacaaa ttctttcgca aacaggaatg cctgctcagg
     7981 gtgtgtttct tgcaacgatt atcggtgcag ttgctggtac cttgatgatg gccttctatg
     8041 ctaacttgcc atacgctcaa gcaccaggta tgggactcaa tgccttcttt acctttacag
     8101 tcgtatttgg acttggttat tcatggcaag aagccctagc tatggtcttc atctgtggga
     8161 ttatttcatt ggttattacc ttgacaaatg ttcgtaaaat gatcattgaa tcgattccaa
     8221 atgcccttcg ctcggctatt tcagctggta tcggtgtctt ccttgcctat gtagggatta
     8281 agaatgctgg acttttgaaa tttacgattg atccaggcaa ctatactgtt gtaggagaag
     8341 gggctgacaa agctcaagca acgattgcag caaactcttc agcagttcca ggcttggtca
     8401 gctttaataa tccagctgtt ttagtggctc ttgcaggact tgccattact atcttctttg
     8461 tcatcaaagg gattaaaggg ggaattattc tctctatctt gacaacaact gttcttgcta
     8521 ttgcagttgg tttggttgat ttgtctagta tcgattttgc taataaccat gttggtgcag
     8581 cttttgaaga tttgaagaca atctttggtg cagctcttgg ttcagaaggt ttgggagctt
     8641 tggtttcaga tacagctcgc ttgcctgaaa ctctgatggc cattcttgcc ttctcattga
     8701 cagatatttt tgacacaatt ggtaccttga tcggtacagg tgaaaaagtt ggtattgtag
     8761 cgacaaatgg tgaaaatcac caatcagcca aattggataa ggctctttac tctgatttga
     8821 ttggaacgac agtcggtgcc attgcaggta cttcaaacgt aacgacttat gttgagtctg
     8881 ctgctggtat cggtgcaggt ggacgtactg gtttgacagc cttggttgta gctatctgtt
     8941 ttgcgatttc aagcttcttt agcccacttc tagcgatcgt accaacagcg gctacagctc
     9001 caatcttgat tatcgttggg attatgatgc ttggtagctt gaaaaatatc cattgggatg
     9061 atatgtctga agcagttcct gccttcttca catctatctt tatgggattc agctactcta
     9121 tcactcaagg gattgcagtt ggtttcttga cttacacttt gactaagctt gtcaaaggtc
     9181 aagttaaaga tgttcatgcc atgatttgga ttttggatgc cttgtttatc cttaactaca
     9241 tcagcatggc cttataatag aatgacccag ggggatttcc cccctttttt aatacaaagg
     9301 agataggtga tgaaagagaa aaatatgtgg aaagaattgt tgaatcgtgc aggctggatt
     9361 ttggtctttt tacttgccgt ccttttatat caggttcccc tagtggttac ctctattttg
     9421 actttaaaag aagtagccct gctacagtca gggctgatag ttactggcct ttcaattgtg
     9481 gttctggctc tatttattat gggagctcgt aaaaccaagt tagctagttt taatttttct
     9541 ttttttagag ctaaagattt ggcacgtttg ggcttgagtt atttagttat tgtcgggtca
     9601 aatatacttg gttctatctt gttgcaactg tcaaatgaga cgacaacagc taaccagtct
     9661 cagattaacg atatggttca gaatagttct ctgatttcta gcttcttctt gctagccttg
     9721 cttgctccaa tttgtgagga aatcttgtgc cgtgggattg ttcctaaaaa gattttccga
     9781 ggcaaggaga acttgggatt tgtagtcggt acgattgtgt ttgctttatt gcatcaacca
     9841 agtaatttac cttctttatt gatttatgga ggtatgtcga cagttctatc ttggacggcc
     9901 tacaagaccc aacgtttgga aatgtcgatc ttgcttcaca tgattgttaa tgggattgct
     9961 ttctgtttgt tggctcttgt ggtgattatg agtcggacat taggaatttc tgtttaaaag
    10021 tttttatgta ggaaccgacc tctttctact agggaaagat gaatgcaatc gtgtccatct
    10081 ttttcttttt atggtaaaat agaaaaataa tatgatgaaa atccttgagg gagtgaccga
    10141 tatgtcaagt aaagccaatc atgcaaagac agttatttgc ggaattatca atgtaacccc
    10201 agactccttt tcggacggtg gtcaattttt tgctcttgag caggcactcc agcaggctcg
    10261 taaattgata gcagaaggag ccagtatgct cgatatcggc ggagaatcga ctcggccggg
    10321 aagtagctat gttgagatag aagaggaaat ccagcgtgtt gttccagtga tcaaagcgat
    10381 tcgcaaggaa agtgatgtcc tcatctctat tgatacttgg aagagtcaag tagcagaggc
    10441 tgctttggct actggtgccg atctagtcaa tgatatcact ggtctcatgg gtgatgaaaa
    10501 aatggcccat gtggttgcta aagctggagc gaaagtggtc atcatgttta acccagttat
    10561 ggctcgacct cagcatccta gttcgcttat cttccctcat tttggctttg gtcaaacctt
    10621 tacagaaaaa gagttagctg actttgaaac attgccaatc gaagacttga tggtggcttt
    10681 ctttgaacga gcactagcga gagcggcaga agctggtatt gcaccagaaa atatcctgtt
    10741 ggatccagga attggctttg gtctgaccaa gaaagaaaat cttctccttt tacgggacct
    10801 ggataaacta catcagaagg gctatccaat ctttctcgga gtgtcgcgca agcgatttgt
    10861 catcaatatc cttgaagaaa gtgggtttga agtcaatcct gagacagagc ttggtttccg
    10921 aaatcgggac acggcttcgg ctcatgtaac tagtatcgct gcgagacagg gtgtagaagt
    10981 ggtgcgcgtg catgacgtag ctagtcacag gatggcagtt gaaattgcct ctgccattcg
    11041 tctggctgat gaagcggaaa atttagattt aaaacaatat aaataagatg aaagaaattg
    11101 aaaacaatca gtggattgct aactaccgga cggatcaacc gcattttggc ttggaacgaa
    11161 tggtggaact gttagctttg cgtggcaatc cccatcttaa actcaaggtt ctccatatcg
    11221 gagggactaa cggcaagggc tcgactattg cttttttgaa aaagatgcta gaaaagctag
    11281 ggctgagagt tggcgtgttt agttcgccct atctcattca ttacacagac cagattagca
    11341 tcaatgggga atcgatctca gaagcgaggc tagaagctct catggcagac tatcagtctt
    11401 tgctggaggg agaagcggtc gccaatttac agggcacaac cgagtttgag attatcacag
    11461 ccctagccta tgactacttt gcctcagagc aagtagatgt ggctatcatg gaagttggca
    11521 tgggtggact tttggatagt accaatgtct gtcagcccat tttgacagga attacaacta
    11581 ttggcttgga tcatgtggcc ctacttggtg acaccttgga ggccatagca gagcagaagg
    11641 caggtattat caaacaaggg atgcccttgg taacagggcg tattgctcca gaagccttga
    11701 ctgtgattga ccgcattgcg gaagggaaag atgcgccgag acttgcctac gggacagatt
    11761 atcaggttcg tcatcaagaa agtgtggtga caggggaagt ctttgactat acaagtgctg
    11821 tcagacaagg tcgcttccag actagcctgc ttggtttgta ccaaatagag aatgctggga
    11881 tggccatagc tttacttgat actttttgtc aagaagatgg tcgagagcta gcaagcaatg
    11941 actttcttgg tcaagccttg gaagaaacaa gttggccagg gcgtttggaa atcgtgtcaa
    12001 gagatccctt gatgattttg gatggagccc ataatcccca tgctatcaag gccttgttgg
    12061 taaccttgca agaacgtttt gcggattatc ataaggaaat cctcttcact tgtatcaaaa
    12121 ccaaagcctt gggggatatg ttggacttgc tgggagccat gccagatacc gagcttactc
    12181 taacacattt tgcggatagt cgggcgacgg atgaaagcgt gctgaaagag gcagctaagt
    12241 ctagaaatct cagctaccaa gattggcatg attttctaga gcagaatttg acagataaaa
    12301 aagaagagaa acaaacagtt aggattgtca caggttcctt gtatttcttg agccaagtga
    12361 gggcctatct gatggagagg aagaacgaga atggatacac aaaagattga agcagctgta
    12421 aaaatgatta tcgaggctgt aggagagaac gctaatcgcg agggcttgca ggaaacacct
    12481 gctcgtgtag cccgtatgta tcaagagatt ttttcaggtc ttggtcaaac agcagaggaa
    12541 catttgtcaa aatcctttga aattattgac gataatatgg tggtagaaaa ggatatcttt
    12601 ttccatacca tgtgtgaaca ccacttcttg ccattttatg gtagagcgca cattgcctac
    12661 attccagatg gtcgtgtggc aggcttgtct aagctagccc gtacggttga agtttattcg
    12721 aaaaaaccac aaattcaaga acgtttgaat atcgaagtgg ccgatgcctt gatggactat
    12781 ctaggtgcta aaggagcctt tgttgtcatt gaggcggaac atatgtgtat gagtatgcgt
    12841 ggtgttagaa aaccaggcac tgcaaccttg acgacagtag ctcgtggtct atttgaaaca
    12901 gataaggacc tccgcgacca agcttatcgt ttaatggggc tataaaaaga atccgtttca
    12961 agcggatttt tctagaaagg aatcattatg gatcaactgc agattaagga tttggaaatg
    13021 tttgcctatc atggtctttt tcctagtgag aaagaattgg ggcagaaatt tgtcgtttca
    13081 gccatcctat cctatgatat gaccaaggca gctacagact tggatttaac agcctctgtc
    13141 cattacggag aattgtgtca gcagtggacg acttggtttc aggaaacgag tgaagatttg
    13201 attgaaacgg tagcctataa actggtggaa cgtacctttg agtcttatcc tcttgtccaa
    13261 gaaatgaagt tggaactgaa aaaaccttgg gcaccggtgc atttgtcact agatacttgc
    13321 tcggtaacca ttcatcgccg caagcaacga gcctttatcg ccctaggaag caatatggga
    13381 gataaacaag caaacttgaa gcaagccatt gacaaactgc gagctcgtgg catctatatt
    13441 ctcaaagagt ccagtgtctt agcgacggag ccttggggtg gagtggagca ggatagcttt
    13501 gccaatcaag tggttgaggt ggaaacctgg ctaccagcac aagacttgtt agaaaccttg
    13561 ttagccattg agtcagagct gggacgggtg agagaagtgc attggggacc tcgtttgatt
    13621 gatttggact tgctctttgt ggaggaccag atcctttata cagacgacct catattgcct
    13681 catccttaca tagcggaacg cctttttgtc cttgagtcct tacaggaaat tgcgcctcat
    13741 tttatccatc cgatattaaa acaaccgatc cgtaacttgt atgatgcttt gaaaaaatag
    13801 aaaaactcta gttttcagtt acttgcaact gaaggctaga gtttttaaaa taggtcattt
    13861 tcttctggga ggaggatagt ttctctaccg tccatgtcta aaaccagtac tcttggggga
    13921 taacgagggt cgaaaggatg gttaaagtca aaatcaatgg ctgtagggag gtgttgactt
    13981 gaaaagtgga aggtaatctt tccttggtta ttaagcaatt gaaactcgag ttcttcttcc
    14041 aattcaaaga cattttttaa gaaatggtcg ataatatacc aaaaagagtc aatgatgtca
    14101 tcaggcaagc tggtaacaat gccaaaacta gcagaccgca tgtgggtatt ggtaaaagcc
    14161 atatctctgt ctcctttctt ttcccttatc atacagcaaa taggattaaa aatcaagaaa
    14221 aggtgatttt ttgaaaagga ttttaactac agggaaaaag aaggaaaaaa attcccaaaa
    14281 atctaccgaa gttaataggt aaattcccaa attaacttga ttatataact ttcagttatt
    14341 ttgagaagtt accgaaaaat attttttata tctattgact tttaggggtg aaatttggta
    14401 tgatagtagg cggtattgtt taccccattt gaaaggcccc ggaaccttcc aaatactttt
    14461 cgatgggaag gaacacccat caccgtaaac aaaaatcgaa ctatatatag gagaaatcat
    14521 gaacaaaaca acatttatgg ctaaaccagg ccaagttgaa cgtaaatggt acgtagttga
    14581 cgcaactgat gtaccacttg gacgtctttc tgcagtagtt gctagcgtac ttcgcggaaa
    14641 aaacaaacca acatttacac cacacactga tacaggtgac ttcgtgattg ttatcaatgc
    14701 tgaaaaagtt aaattgactg gtaaaaaagc aactgataaa atctactaca ctcactcaaa
    14761 ccacccaggt ggattgaaac aaatctctgc aggtgaactt cgttctaaaa atgcagtacg
    14821 tttgattgag aaatcagtta aaggtatgct tccacacaat acacttggac gcgctcaagg
    14881 tatgaagttg aaagtatttg ttggagctga gcacactcac gctgcacaac aaccagaagt
    14941 tcttgacatt tcaggactta tctaaggaaa ggaacaataa agtatgtcac aagcacaata
    15001 tgcaggtact ggacgtcgta aaaacgctgt tgcacgcgtt cgccttgttc caggaactgg
    15061 taaaatcact gttaacaaaa aagatgttga agagtacatc ccacacgctg accttcgtct
    15121 tgtcatcaac caaccattcg cagttacttc aactgtaggt tcatacgacg ttttcgttaa
    15181 cgttgtaggt ggtggatacg ctggtcaatc aggagctatc cgtcacggta tcgctcgtgc
    15241 ccttcttcaa gtagacccag acttccgcga ttcattgaaa cgcgcaggac ttcttacacg
    15301 tgactcacgt aaagttgaac gtaagaaacc aggtcttaag aaagctcgta aagcatcaca
    15361 atttagtaaa cgttaattcg aaagaattac tatacttata cagagcacct ttcggggtgt
    15421 tcttttttta tactttctta ctaaattggt gcaattgaca cagttgttgc gactttagtc
    15481 gcttacaaat gtggctgcaa cctgacaagg tcagttgcct caaaacgtta atcaatacaa
    15541 ttatatcaac gtttcaaagc actcaagagt ttacctcatg ggtgcttttt ttatgttttt
    15601 tgaaaaagtt cacctcaaag tttaccttat tttcacctta ttgttttaga gactgtaagg
    15661 caaattcacc gacggccata gggactacat tagaagtatt gacataaatc tgtgttgtac
    15721 ctgtgtcgct atgtattaga gcttcagaaa tggcttctaa gctcattcct gcttgtttag
    15781 cgagcgttgc tccggtatga cgtagtttgt gtggcgtagc gtgtgtaagc tctgggtgtc
    15841 gttttctgat agttttcatc ttattattga gatagtcaga atgtaaaggt ttattgatat
    15901 tgcctttggt atctatgtaa gtaaagatga attgttctgg attactgatg ataccaaact
    15961 ttgccagttc atatttttgt tgttttttcc atgaagtcag aagttgaagt aagtcattag
    16021 aaatagagaa aatgggaaat gtttccacaa taaaacgcat attatcagat ggttaatgcg
    16081 ttttatattt gggtttgagg cttataaaat aggataagcg tactgaaaac ttagatatga
    16141 caaacaaaat aaaattttta aaagctacat agaaacatgg tataatgtat ctaaaggagg
    16201 tagcatatgt ccttagatat agataaagaa aagatgacaa tcatgggaat agcttttgaa
    16261 aaccgatccg tttttaagag tgtttggtat gctttaagta caaatatgat tgaaggatgg
    16321 cgacctacgg ttagtgatgt tgaaaaatta cgagatgaag ctcttgctct ggggatgact
    16381 taatgaagcg caactgttat gaatcttatg actatgttga tccggataat ttatataccg
    16441 atccgggttc ttcagttcta agaaataagc aggaaggaaa aaagcacgcg agcttgaata
    16501 tcgtatgatt gctagtaaga gtttgaaatt atttatcaat cctatcttag ttaatagttt
    16561 aaaagtagtt tgtttcacaa ctgcataagc ttagaaaagt tgaaaagtga taactcttta
    16621 ataaactctt ggtttgttaa aagatatttt gtccactaca tttttttcaa ttgactatta
    16681 tttgtcatag atataacaga tagaacattg cattcagaat aatgaaaaac gcatcaaaaa
    16741 tggtgcgttc tgtttttatg ctaccataaa aggaggaaaa caaattgaaa atttttaagg
    16801 gagagtttta tcgaatctct gtattaacag acaagctagt aaggttagaa tactctcgaa
    16861 ctggaaattt tgaggataga acgacacaac ttatctataa tagagatttt ggccaagttt
    16921 cgttagatta tatcgagaca tcaaatgtac tagatattat gacggactat tttcatctgc
    16981 actttaataa aggagaattt aacgccgaaa atttatttat agaattaaaa ggaaattttg
    17041 ccgtatatgg tagtcgctgg tattttggtg aatctattga aacgttaaaa ggaacagctc
    17101 ggactctgga taaggcagat ggagcaatct cgttagaaga tggaattatt agccgaaatg
    17161 gtatagcctt attggatgat tctcaaggat ttatttggga tgaacaatct ggttatattg
    17221 agagagaaaa tcaaattgac ctgtatttct ttgcctatgg gcatgattat agaggagcaa
    17281 tcagagactt ttaccatttg actggttcaa cacccttgtt gccaagatat gctttaggca
    17341 attggtggag tagatattgg ccttatacgt cggatgaata cttggattta atagacagat
    17401 ttgaaacaga gaaaattcca ttatctatcg gtgtgttaga tatggattgg catataactg
    17461 acattccagc tcgttttgga agtggctgga caggatatag ttggaataga aacttaatac
    17521 caaatccaga acagttattg caacaacttc atgatagaaa gttaaaactc tccttaaatg
    17581 tccatcctgc tgatgggata cgggcttatg aagaagctta tcctcaaatc gcaaaacgat
    17641 tggggttaaa tgtagaacta gaagaacctg ctatttttga tttttttaat ccctctttta
    17701 gggaagccta ctttaaagat gttcattatg agttagaaaa gcagggagta gatttttggt
    17761 ggattgactg gcaacaaggg acacaaggta tgctagatcc actttggctt ttaaaccatt
    17821 atcactatca ggatagttgt aaaaatgcag aaggtggttt gattttatca agatatgcag
    17881 gtcctggtag tcaccgctac cctgttggtt tttcagggga tactattatt agttggaatt
    17941 ccttaagatt tcaaccctat tttacagcga cagcatctaa tatcggttat agttggtgga
    18001 gtcatgatat cggtggacat atgttggggg attatgacga agagctacaa actagatggc
    18061 tacagtttgg tgtttttagt ccgattactc gattacatag ttctagaagt ccttttaata
    18121 gtaaagaacc ttggtttttt tcagaaacaa catctaagat tatgaagaaa tacctccgtt
    18181 tgagacatca gatgattccc tatctatata ccatgaatgt aaagacacat gaggaaggtg
    18241 ccccattaat cagtccaatc tattatttct atccagagaa tgatgagagc tataatgttc
    18301 caaatcaata cttttttgga acagaactga tggtggctcc cattgtagaa aagatggatt
    18361 tgacattcca atctgcaaaa gtagatgtat ggttccctga aggtgaatgg tatgactttt
    18421 tttcagagaa aaaatacaca ggtggtgtga agttaagtgt ttatagagac atctcgacta
    18481 cgcctgtgtt tgcaaaaagt ggtgcaatca ttcccttggt tggttctgag ataggtatgg
    18541 gtgttgattt acctgaagtt gtagattggt atgtattccc aggaaaacaa cattcttttg
    18601 aaatgcttga agatcaaaat ggtcaaagat ataaaacaag attatcaatc gattgggaaa
    18661 tgggaatggt agagttagca ttacaaggag attctagtat cgttccaagc aatagaaaac
    18721 atagaattca ttttaaagga acgaatgtgt ctataattga attgccaaat aagaatgata
    18781 cagctaaatt tgaatggaaa gataataaaa ggacatcttt aaatgacgaa gtttttagac
    18841 tactaaagac agcttctctt ccatatgaat taaaagatag attgttaaat caattcatca
    18901 atgcgaaaaa ctctcatgac ttaatgaata tcttgcatca tcaggataag gaattgagag
    18961 ggcgtttgtt ggaaatgata tttactagcc aaaactaatt gaaataatta gaaagtcatt
    19021 tgtttagtgt atatgaagat tctgtatggt attcgttatc actggtcaca ttgaaaatgt
    19081 gaaataagca cctacaacat agactttaca acacttcatg ttttatatca tggggtgttt
    19141 ttctatttaa gtcaaagccc atcaaaaaat ctcactattc atcaggttaa aggaaaccta
    19201 atcatagtga gattgtgaat tatagtagag tttgtatcgc ctcttcaatt tgttttgggt
    19261 ctgtttttga agaaaagcgt tcaaagactt gcccatctcg accgatgaga aacttagaga
    19321 aattccattc gattcgtttt cctagtgggc cggatttctg gtcttttaac cagacataga
    19381 gagggtctgc ttccttaccg ttgaccttaa tcttggcaaa acgtgggaag gtggtttgaa
    19441 aatgtaggct acagaaggcg ttgatttcct ctgcgctgcc gggtgcttgt cccataaact
    19501 gattgcaagg gaaatccaat atttcgaagc cctgagcttg atagcgttca tagagttctt
    19561 gaagtccctg gtactggggc gttaaaccac atccagtagc agtgttgaca atcaagagaa
    19621 ctttgccacg atagctatcc aagggagttg cttggttgtt ttggttcaaa acggaaaaat
    19681 cataaagtga agtcatgagg gcctcttttc tttttctaca ttgtaacagt ttttactttt
    19741 ttcgtcgagt cggatggaag atattgttga agaataagtc ttcaatatga tagaaaccgt
    19801 ttacaaataa ctttaaaaat ggtatgattt aattaaaccg gtttacttag ttgctataaa
    19861 tctctaattt gagatttagt tcctattgat tttacaatat gtttattgga gtgtatacat
    19921 gcaaacaaaa acaaagaagc tcattgtgag tttgtcttca cttgttttat caggattttt
    19981 attaaaccat tatatgacag ttggagcgga agaaacgact acgaatacca ttcagcaaag
    20041 ccagaaggaa gttcagtatc agcaaaggga tacaaaaaat ttagttgaaa atggtgattt
    20101 tggtcagacg gaggacggaa gcagtccgtg gacaggaagc aaagctcagg ggtggtcagc
    20161 ttgggtagac cagaagaata gttctgcaga tgcctcaact cgagtcattg aggctaaggg
    20221 tggggctatc actatctcaa gccatgagaa attaagggca gcggttcacc gtatggttcc
    20281 tattgaagct aagaaaaagt ataaactgcg tttcaagatt aaaacagatc ataaagtcgg
    20341 gattgccaag gttcgtatca ttgaggaaag tggtaaggac aagcgattgt ggaattctgc
    20401 aacgacgtca ggaacaaagg actggcagac cattgaagca gactatagcc cgactttaga
    20461 tgttgataaa atcaagctgg agttattcta tgaaacagga actgggactg tttcctttaa
    20521 ggatattgag ctggtagagg tagcagacca gctttctgag gattctcaaa cagataaaca
    20581 gcttgaggaa aagattgatt taccaattgg aaaaaaacat gttttttctc ttgcggacta
    20641 tacttataag gtagaaaatc ctgacgttgc ttcagtcaaa aatggaattt tagaacctct
    20701 taaggaaggg acaaccaatg tcattgtcag taaagatggc aaggaagtga aaaagattcc
    20761 tttgaagatt ctagcctctg ttaaggatgc atacacagcc cgtttggatg actggaatgg
    20821 catcatcgct gggaatcaat actatgattc taaaaatgaa cagatggcca aattaaacca
    20881 ggaattggaa ggaaaggtag ctgatagcct atctagtatt tcaagtcagg cggaccgcac
    20941 ctatttgtgg gaaaaatttt caaattataa aatgtctgca aatctgactg ccacttatcg
    21001 gaaattggag gagatggcca agcaagtgac caatccttct tctcgttatt atcaagatga
    21061 aactgtcgtt cgaacagtca gggattccat ggaatggatg cataaacatg tctacaatag
    21121 tgaaaagagc attgttggga actggtggga ttatgaaatc ggtacacctc gtgccatcaa
    21181 caataccttg tctctgatga aagaatactt ctctgatgag gaaattaaaa aatatacaga
    21241 tgtgattgaa aaatttgtac cagatcccga acatttccga aagacgactg ataacccatt
    21301 taaggctcta ggtggaaact tagttgatat gggaagagta aaagtaatag ctggtttact
    21361 gcgtaaggat gatcaagaaa tttcttctac cattcgctcg attgagcaag tgttcaagtt
    21421 ggtagaccaa ggtgaaggtt tttatcaaga tggatcctat atagaccata ccaatgttgc
    21481 ctatacgggt gcttatggga atgttttgat tgatggcctg tctcaactgt tgccagtcat
    21541 tcaaaagacc aagaatccaa tcgataaaga taaaatgcaa accatgtact actggattga
    21601 taaatcgttt gctcctttgc tggtgaatgg agagctgatg gatatgagtc gtggacgctc
    21661 gatcagtcgt gcaaatagcg aggggcacgt ggccgcagta gaagtactaa gagggattca
    21721 ccgaatagcg gatatgtctg aaggagaaac caaacaacgt ttgcagagtc ttgtgaagac
    21781 cattgttcaa tcggatagtt attatgatgt ctttaagaat ttgaagactt ataaggatat
    21841 cagtttgatg caatccttgt taagtgatgc aggagtcgca agtgttccaa gaacaagtta
    21901 cctatctgcc tttaacaaga tggataaaac agccatgtac aatgcagaga aagggtttgg
    21961 atttggcttg tcactctttt ccagtcgtac cttgaattac gaacacatga acaaggaaaa
    22021 taaacgtggt tggtatacga gtgatgggat gttctatctt tacaatggcg atttgagtca
    22081 ctatagcgat ggctactggc caacagttaa tccatataag atgcctggta caacagagac
    22141 ggatgctaag agagcggata gcgatacagg taaagtttta ccgtctgctt tcgttggaac
    22201 gagcaaacta gatgatgcca atgcgacagc aaccatggat ttcaccaact ggaatcaaac
    22261 attgactgct cataagagct ggtttatgct aaaggataag atcgcctttt taggaagcaa
    22321 tatccaaaac acttcaacag atactgctgc aactacaatt gaccagagaa aactggaatc
    22381 aagtaatcca tataaagtct atgtcaatga taaagaagcc tcccttacag aacaagaaaa
    22441 ggattatcct gaaacccaaa gtgtcttttt agaatcatcc gattcgaaaa agaatattgg
    22501 ttactttttc tttaagaaga gttcaatcag tatgagtaag gctttgcaaa agggagcctg
    22561 gaaggatatc aatgaaggac agtcagacaa ggaagttgaa aatgaatttc ttacgattag
    22621 tcaggctcat aagcaaaatg gagattctta tggctatatg ctcatcccta acgtgggtcg
    22681 tgccaccttc aatcaaatga taaaagagtt agaaagtagc ctcatcgaaa ataacgaaac
    22741 ccttcagtct gtttatgatg ccaaacaagg agtttggggc attgtgaaat atgatgattc
    22801 tgtctctact atttccaacc aattccaagt tttgaaacgt ggagtctata ccattcgaaa
    22861 agaaggggat gaatataaga ttgcctacta taatcctgaa acccaggaat cagctccaga
    22921 tcaggaagtc tttaaaaagc tagagcaagc agctcagcca caagtacaga attcaaaaga
    22981 aaaggaaaaa tctgaagagg aaaagaacca ttcggatcaa aagaatctcc ctcagacagg
    23041 agaaggtcag tcaatcttgg caagtctagg gttcttgcta cttggggcgt tttatttatt
    23101 ccgtagagga aagaacaact aatttgttca tcatctagat gaattacatg aaactgtgga
    23161 aaatagaaaa aatccgaaga aggccaaata tcttcggatt tttcttttaa aaaaggataa
    23221 gttgatactt atccttttta cttcattatg tgactatagc gttttgaaac tagaatagga
    23281 catcataact gctaaaagat ttctataaat tcatttgatt ttcctaatca atttgttcgt
    23341 atcctatttc actctactat attagtccgt ctcgcacctc acggagcgag acaaaacaac
    23401 cacccgctat gcgggtgcgc gtcgaaggtt ataccaaaaa aactccaaac gcgatacaat
    23461 aaaggtgttc aacccacttg taaagcgaaa ggagaaaaaa tatggcacaa aaggctcata
    23521 gtttatctca cacaaagtgg aggtgttcta tcacattgtg ttcaccccta agtatagacg
    23581 aaaagtcatc tataatcaat atagaagtag tttaggcgaa atatttcatc gcttgtgtag
    23641 ttataaaggt gttgaaatga tcgagggtca cttaatgcca gaccatgtac acatgttagt
    23701 cagtattcca ctaaggctaa gtgtttcgag tttcatgggt tatttaaaag gcaaaagtgc
    23761 actcatgatg tttgacaaac acgccaatct caagtacaag tttgggaatc ggcatttctg
    23821 ggcggaaggt tattatgtaa gtccagtagg gcttaatgaa gacacaatta agaaatatag
    23881 tcaagattaa agaaattatc gagtggatga tttcttcacg agtatgaaaa tttgagaact
    23941 agtaaagtat gatatatagt aaaatgaaat aagaacagga caaaatcgat caggacagtc
    24001 aaatcgattt ttaacaatgt tttagaagta gaggtgtact attccagttt caatctacta
    24061 tagcactaga taaattgagt gtaaaagaat atgaggatcc ctttagggat agtggtaagt
    24121 aataccaaag tctcttaaag aggcaagtga cgagtcaaga gcaataaggc ttgaacaacg
    24181 tgaaagccag cgtctttagg cgctggctga tgatttgggc ttatagctct gagataaacc
    24241 acccgttaga cgggtagtta tgattttatc tgagtgtaac atactgttgg gcaatctcgc
    24301 tgatgcggtc aaagttgcct tgggaagcga gtttattgag ttcgccacca attccaacgg
    24361 catctgcacc agcagcgaac cattgaggga tgttgtttag accgactcct ccggttacca
    24421 ttacggaaac ttgtggaatc ggtgccttga ctgcagagat atatgctgga ctgagagtac
    24481 tacctgggaa gagtttgatg atttcactac cggcttcaag tgcagtcgtg atctctgtga
    24541 gggtaataca gcctggaatg tacggtgtgc tgtagagatt gcacattttc gcagtttcag
    24601 catggaaaga tggagaaaca acgtaatttg ctccggctag aatggcatct ctagcagtta
    24661 cggcatcaag cacagtacct gcaccgatac aaacactctg atcgtcctga tacaagtcta
    24721 caagttcctt gatgatttgt cctgcatact gattggtata ggcgatttca atagctttga
    24781 taccgccctt gatacaagca atcgaggctt gtagtccttc ttcctttgta tttcctcgaa
    24841 tgacagcaac aattttctgt ttttttagtt caataattgt atctgatttg gtcatgtaat
    24901 tctcctaacg aatgatatct tgtgcatttg ccagtaaatt ttcaatacta gttgcggaag
    24961 tggagagatg gtctcctgga agagtgcatt tgagagttgc gctcgcaact gcaaagtcaa
    25021 tggtagtttt tagggaggaa tgatggagta gttggtaaag ggcaccagat ataaaggcat
    25081 ccccgctacc aattcgttga tagactgcag tttttagttg gacagactct tcaaatcgtt
    25141 cttccagagc ataggcttga tagacatttt tgtcttgctc atcactagag cggagggtat
    25201 ggaaaatggc cttgaaacca taggcttctt ttaaaagtcg catgcgattt tccacctctt
    25261 ctaggctagc actgtctctt ggaaacatct ctagattttg gtcatcaatc atgagaggat
    25321 caataccgaa gcaatagtca gtaaaacgtg caaacttaga aaattcatac ttggcttcta
    25381 ggactgaaat catctttgtt ctcagattga gatccattga aacgacaatt cctcggcgct
    25441 tggcttcttc caagagtagg agaaggatcg cacggacctc ttgaccgata gctacggtga
    25501 ttccactaaa atgaaaatgg ctaatcccct gaaagagaga atccatatct agcatgtttg
    25561 gccgaatctg gctgatactc gtatgcttac gatcgtagaa aacttcactt tgacgacaac
    25621 caaagccgtt ctccaaatag tagaggccga ttcgatcgcc aagccgacag attgaactgg
    25681 tatcgatttg gtgctgtttc aagaatgtga gaaaacgatc tccaatctcg ttggcaggga
    25741 gtgcggtaaa gactttcgtt gagataccca gggcttgcaa attacaagcg atgttaattt
    25801 ctgatccgcc aaaataagtc gaactggcaa catgatctcc gatactggtg gcatctaatg
    25861 gtgagattcg aattaatggt tcgccaaaca gtaatatttt cgtcatccat tagttccttt
    25921 ctggtttaga atagctttac ttggctaata cttctttcaa ataagcagcg acttcatcat
    25981 cttggcagtt ttcaaagaag tattcttgga agtatttgcc agatatggtg tctttgagga
    26041 agtcttggtc gattgtttta aggatggtca tcaaatcatt gtgggtgatt tgtttcacct
    26101 cgtttaagat acgagcgttg cgttgttcag ggattacacg ttctcttggg tagccaccgc
    26161 ccatttcttc agcaaataag cgttcaaaca tcaatttgag ggtcagttct gccccccagc
    26221 caaatccttt ggcataaggg atagacaagg cgttaccacc attgatttga gaataaaggt
    26281 aagcgtcagt tgggtccact gctagaccac agacaacacc agggaagctg tttaaagcaa
    26341 gcatagcccc tacacccgta ccacagccgg taacaacaaa gtcaactgcc tttgtattta
    26401 aaaggatggc agccattagt ccgttctgca cataagttaa ttgactttct ccttcttctc
    26461 cacgcatacc atagttaaat aattggtagt cttttttatc tgtcgcttct tttagactat
    26521 cgtaaataat gtgattcttg ctagcttgac tattttcatt gattaatgcg attttcataa
    26581 gatcttcttt ctatttttac tcaggttgtt ttccgatgta ggctaagata ccgccatcta
    26641 catataggat gtggccattg acaaaattgc tggcatcact agcgagaaag acagcagggc
    26701 ccatcaaatc ttcaggattt ccccaacgtg cagcaggtgt ttttgcaatg atgaactggt
    26761 caaatgggtg gcgagaacca tcttcttgca actcacgaag aggtgctgtt tgaggagtgg
    26821 caatataacc cggtccaatt ccgttacatt ggatattggc tccaccgtat tcagacgcaa
    26881 tgttgcgggt caacattttc aagccccctt tagcagcagc ataagcgcta actgtttcac
    26941 gtcccagttc gctcatcatc gaacaaatat tgataatctt tccatgccct ttctttatca
    27001 tagaaggaat aactgccttt gaaacgataa atggtgcgtt taagtcaata tcgatgacct
    27061 tacggaaatc agcggcgctc atttcgcaca ttggaactcg gcggataata ccagcgttat
    27121 taacgaggat gtcaatgaca ccaacctctt gttcgatttg cttgaccatg gcttggatac
    27181 cgtcctcgtc tgtcacgtca cagacatatc catgggcttc gatgccaact tcacgataag
    27241 cttcaatccc tttattgacc agatcttgat tgatatcgtt aaagacaata gtagcgccgg
    27301 cttcagcgta ggatttggca atagcaaatc caattccata agaagcgccg gtgatgagag
    27361 caatttttcc ttttaaagaa aactgctcaa ttgagaatga tgtatttgtc atttgatact
    27421 tcctttcaaa tttgttaaga cttctctata accaaattat attctagtaa accggttttg
    27481 tcaataaata aatattttta tttataaaaa gatttatttt ccttaaaaat ggaatatttt
    27541 gaaatttctg tctattaaaa tggttaaaaa gataatgaaa gcgctaatat ttttttaaaa
    27601 aaactattga caagataaaa ataaaggatt acaatgggga atataaagta aaccggtaaa
    27661 cctaaacaag aaaggagaaa agatgaaaat tgtacttgta gggcatggac attttgctac
    27721 agggatttat agttctttac aattgattgc aggcaatcaa gaaagtgtgg aggcgattga
    27781 ctttgtggaa ggaatgtcag cagatgaact caagcaaaaa atcttacttg caatttcaaa
    27841 tgaagaagaa gttttaatcc taagtgatct cttgggagga tcgccattca aggtttcttc
    27901 taccataatg ggagaaaatc cagccaaaac aatgaatgtt ctctcgggtt tgaacttagc
    27961 catgttaatg gaagcagtct ttgctagaat ggctcatagc tttgatgagg ttgttaataa
    28021 atcagtagtg gcggcccagg gcggagtcgt aaatggtaaa gaattgtttt caacggatgc
    28081 agaggaagag gaagaagatt tcgaatcggg tatttaaagg gtaaaagaat gataaaaaag
    28141 gttacgattg aaaaaataaa atcgcctgag cgcttcttag aagtaccact tctgacgaaa
    28201 gaagaagtcg gccaggcaat cgataaggtt attcggcagt tagaactcaa ccttgactat
    28261 ttcaaggaag atttccctac gccagctacc tttgataatg tctatccaat catggataac
    28321 acggaatgga ccaatggttt ctggacagga gaactgtggt tggcttatga atacagtcaa
    28381 caggatgcat ttaaaaacat cgctcataaa aatgttcttt ctttcctgga tcgtgtcaat
    28441 aagagagtag aattggatca ccatgatctc ggcttcttgt acacaccgtc ttgtatggct
    28501 gaatataaga taaatggaga tggagaggct agagaagcaa ccttgaaagc tgcagataag
    28561 ttgattgaac gctatcaaga aaaaggtggt tttattcaag cttggggaga cttgggcaag
    28621 aaagagcatt accgtttgat tatcgactgc ttgctcaata tccaactctt attctttgct
    28681 tatcaagaaa caggcgatca aaaatactac gatattgcag aaagtcattt ctatgcttca
    28741 gctaataatg taatccgtga tgacgcttcg tccttccaca ccttctattt tgatcctgag
    28801 acaggtcaac cctttaaagg tgtaacgaga caagggtata gtgatgattc atgctgggca
    28861 cgtggtcaat catggggagt ctatggtatt cctttgactt atcgtcactt aaaagacgag
    28921 tcctgctttg acttgtttaa gggtgtgacc aattatttct tgaatcgtct gccaaaagat
    28981 catgtgtcct attgggattt gatttttaat gatggtagtg atcaatcacg agattcttca
    29041 gcaacagcta tcgccgtctg tgggattcat gaaatgctaa aacatctccc agaggtggat
    29101 gctgacaaag atatttataa acatgctatg catgccatgc ttcgttcctt gatcgaacat
    29161 tatgcaaatg atcaatttac ccctggtggg acaagtctcc tccacggtgt gtactcatgg
    29221 cattcaggta aaggagtgga tgaaggcaat atctggggtg actactatta cctagaagcc
    29281 cttatccgtt tctacaaaga ctggaaccta tattggtagg aggagaaata tgacaatgcc
    29341 aaatattatt atgacccgta tcgatgaacg gttgattcat ggacaaggac aactttgggt
    29401 aaaataccta ggttgtaata cggtcattgt tgccaatgac gaagtaagca cggacaagat
    29461 gcaacaaact ctgatgaaaa cagttgtgcc agactcagtt gccatgcgtt tcttcccttt
    29521 gcaaaaggtg attgatatca ttcacaaggc taatcctgct caaacgatct ttatcgttgt
    29581 aaaggatgtg aaggacgctt taaccttggt agaaggtggt gtccctatca aagaaatcaa
    29641 tattgggaac attcacaatg ctactggtaa agagcaagtg acacgctcca tcttcctggg
    29701 tgaagaggac aaggcagccc tcaaggaatt aagccaaact catcaagtaa catttaatac
    29761 gaaaacaact ccaacaggaa atgatggagc tgttcaagtc aacattatgg actatattta
    29821 aaagaggaga tcgttatgtc gattaatgta tttcaagcga ttttaattgg attatggaca
    29881 gctttctgtt ttagtggaat gctattagga atttacacca atagatgtat tgttctgtca
    29941 tttggtgtcg gaattattct aggtgatctg cctactgctc ttgcaatggg agctattggt
    30001 gaattggctt atatgggatt cggtgttggt gctggaggta ctgttccacc aaacccaatc
    30061 ggacctggta tctttggtac cttgatggct atcactagtg ctggtaaagt cagtccagaa
    30121 gcggctcttg ccctctctac tccgattgct gtggcgattc aattcttaca aactttcgcc
    30181 tacactgtac gtgctggtgc gcctgaaacg gcgatgaagc acttgaaaaa ccataatttg
    30241 aagaaattca agttcactct aaatgcaaca atttggttgt ttgcctttat tggatttacc
    30301 ttgggttgct tgggtgccct ttcaatggat accttgttga aactcgtaga ctacattcca
    30361 ccggtattgc ttacaggttt gacagttgct ggtaaaatgc tcccagctat cggttttgcg
    30421 atgatcttgt cagtgatggc taagaaagag ttgattccct ttgtcttgtt gggatatgtt
    30481 tgtgcagctt atctaaacat cccaacaatt ggtattgcaa ttgtaggtac tatctttgct
    30541 ttgattgaat tttataacaa gccaaaaaca gcggatcatg tggtagagga ggaagcacac
    30601 gatgactgga tctaacaaat taacaaaacg tgattatctt aaaacgtctt tgcgggcatt
    30661 cttttgtcaa aatggattta actatagtaa ctatcaaggg ttgggatatg ccaatgtgat
    30721 gtatcctgct ttgaaaaaac actatggaga gaatcaggaa ggtttctacc aagccttgga
    30781 agaaaactgt gaattctata ataccaaccc acacttcctg ccttttatta ccagcttgca
    30841 tcttgtaatg ttggaaaatg gccgtccggc aaaagaaaca cgtagcatca agatggcctt
    30901 gatgggacca ttagcaggta ttggggattc tctctctcaa ttctgtttag ctccattgtt
    30961 ctcaaccatc gcagcttcgt ttgctcaaga aggcttggtt gtcggtccaa tcttgttctt
    31021 ccttgcgatg aatacgattt taacagcgat taaattgtca actggtctgt atggatacaa
    31081 actaggaacg actgtgattg ataaactaag cgagcagatg gcaacgattt ctcgtattgc
    31141 caatattatt ggtgtaaccg taattgctgg tttggcagcg acatctgtta agattatggt
    31201 accgatcacc tttgctgcag gggaagttaa agcagacgct aaacaaagta tcgtaagtat
    31261 tcagggaatg cttgataagg ttgctccagc tcttctacca gccctattta cacttttagt
    31321 ttactacttg atcaaagaaa agaaatggac aacatataaa ctcgttattt taacagttat
    31381 catcggaatt atcggaagct ggcttaagat tatagcttaa tggatacaac attgttttat
    31441 ggaatagtga ttgtcttggc agtgagtccc cttttactgt caagctttca ttctattcgt
    31501 cagcaaaagt tgcttcgcaa acagatggag caacgacaag agtatttagc ttctttaaca
    31561 tctggtgatg aagtgttgtt attgtctgga attcatggaa aaatcatttc tatccaagat
    31621 gacttggtct ccttgcagat tgcaaaaggt gtggtcatct atgtagaaaa ggaaagtgta
    31681 atgggaaaga caaaagaact gctttttaag tagttctttt tctggtaata gataatgaaa
    31741 gaagaaagaa gacaattttt tgaaagaatc gatggaaacc aatgtcgtga ttatatcttg
    31801 tcccactgtt caaaagacta tgagaaagtc aagagttccc ttgaacgctt gatggacaat
    31861 cgttttatgt ttgatagtcc ttgggatatg gagccttgtt caaaaatcca tcaaatccag
    31921 ccgatggtat gggatcaagt atttgaagat gatccagaat ggtcttatat gctcaatcga
    31981 caagaatatc tcttgcagtt tatgataggg tatgtggtag aaggagataa ggactatatt
    32041 caaaagtgca agttctttct atttgattgg attgagcagg tgagagaatt ttctcctcaa
    32101 tccttgatga ctagaacctt ggataccggt attcgttcct ttacttggtt gaaactactc
    32161 ttgctcctct tgaaatttga cttgctagag gagaaagaac tagagaaaat tttggtcagt
    32221 ctagaaaagc agattgactt tatgaaaagc cactatcgcg ccaagtacac ccttagtaac
    32281 tggggaattt tacaaacaat tccgatgctt gctatctatc atttctttgc agataagatg
    32341 gacctagaag aagcttacca ttttgcttca gaggagttga aacagcaaat tgagacacag
    32401 attttaggag atggaagcca gtttgaacag tcgattctct atcatgtaga ggtttataaa
    32461 gccttgctgg atttgtgtct cttgcttcca gacttgcaag atagttacca agagttgctg
    32521 gaaaagatgg cgacctatat tcaaatgatg acaggcttag atggacgaac tttggctttt
    32581 ggtgatagcg attctacaga aacgacagaa atgttgagcc tgtctgctgt ggttttgaac
    32641 aaggaagacc ttcttaacgg tctggatgtt aaagtcgatt tgcttagcct cttgttcctg
    32701 gggcgagaaa aggtcaagcg actgcaggaa tttgaaaagc gagcttggca gcctaagttc
    32761 atgagctttg aagactctgg acatgtctgc attaaggatg aacatcgtta tctatttttc
    32821 aaaaatggtc cgctaggaag tgcccatagc catagtgacg agaatagttt ttgcttacag
    32881 tatcaaggcc aacctatttt catagatgct gggcgttatt cttatcggga gatatatgaa
    32941 cgttatctct taaagagtgc ttggagtcat tcgacctgca ttgtagatgg gaaagctccg
    33001 gaaagaatca cgggatcctg ggaatatgaa tactatcctc actccctgtt ttgtcaccat
    33061 aaagaaaggg agggaatgca ttatattgaa ggggcttatt ggtcagcaga acctgatttg
    33121 ccttatcttc acaggagaaa aatcctcatg ttggtagagg atgtctggct cttggtagat
    33181 gacatcaggt gtcaaggtca gcatgaggcg ttgactcagt ttatccttga caaggatgtg
    33241 acctatcaag atgggaaaat caatcagttg agactatgga gtgaagttga ttttgatttg
    33301 gaagatacca tcatttctcc taaatacaat gagcttgaaa gaagtagcaa actcaccaag
    33361 cgccaattct ttgagaatca gatgctggat tataccatca ttgcgcatga gagttttgaa
    33421 atcatccgtc attctgtcta ccagacagat gatcgtgaag tggaaaatgc tctggctttt
    33481 gaagtgaaaa atgacgaaac agacaagctg attctgttat taagcgagga tatttgtgta
    33541 ggtgaaaaat tgtgcctcgt tgacggaaca aaaatgcgtg gaaaatgtct agtatatgat
    33601 aaaataaatg agagaatgat tcgcttgcag tgctagaaat aggcattttg aatagtgaat
    33661 atgttataat aagtattagt aggaggtgtt ttagattgga gaagaaactg accataaaag
    33721 acattgcgga aatggctcag acctcgaaaa caaccgtgtc attttaccta aacgggaaat
    33781 atgaaaaaat gtcccaagag acacgtgaaa agattgaaaa agttattcat gaaacaaatt
    33841 acaaaccgag cattgttgcg cgtagcttaa actccaaacg aacaaaatta atcggtgttt
    33901 tgattggtga tattaccaac agtttctcaa accaaattgt taagggaatt gaggacatcg
    33961 ccagccagaa tggctaccag gtaatgatag gaaatagtaa ttacagccaa gagagtgagg
    34021 accggtatat tgaaagcatg cttctcttgg gagtagacgg ctttattatt cagccgacct
    34081 ctaatttccg aaaatattct cgtatcatcg atgagaaaaa gaagaaaatg gtcttttttg
    34141 atagtcagct ctatgaacac cggactagct gggttaaaac caataactat gatgccgttt
    34201 atgacatgac ccagtcctgt atcgaaaaag gttatgaaca ttttctcttg attacagcgg
    34261 atacgagtcg tttgagtact cggattgagc gggcaagtgg ttttgtggat gctttaacag
    34321 atgctaatat gcgtcacgcc agtctaacca ttgaagataa gcatacgaat ttggaacaaa
    34381 ttaaggaatt tttacaaaaa gaaatcgatc ccgatgaaaa aactctggta tttatcccta
    34441 actgttgggc cctacctcta gtctttaccg ttatcaaaga gttgaattat aacttgccac
    34501 aagttgggtt gattggtttt gacaatacgg agtggacttg cttttcttct ccaagtgttt
    34561 cgacgctggt tcagccctcc tttgaggaag gacaacaggc tacaaagatt ttgattgatc
    34621 agattgaagg ccgcaatcaa gaagaaaggc aacaagtctt ggattgtagt gtgaattgga
    34681 aagagtcgac tttctaaaat gaaggaaaat gacttgcaat ctctgttaag aaataaaata
    34741 atcccaccta gaacaagcta ggtgggatta tttgcctatg aaatgagaaa ttatgggagc
    34801 aagctcctaa atcaactgtt tttgatctac ttctttaact acttgataaa agttatagaa
    34861 gtaggccaaa cttgaaatga tggttacgac taggaatatt gaaaatttcc attggacagg
    34921 gttggttaaa agttgtggaa aggatatgag gagaaagaag agggctgcgt tgaggacagg
    34981 tatccgtttt gattgtattt tctcaagtcc tttattgagc gcaggaagaa agaggagtag
    35041 gagtagtaaa actgtatgag aaacagctcc tgaaataagg gcaaagaaaa ggaaaatact
    35101 gataaaaaca tgaatgatca gtagtctagc tagtgatttc ataaggtacc tcctaatcct
    35161 ggtctttttt agctcttgca atacgaagtg agtcgacaat atgtatcatc actccgaaaa
    35221 agaaagctcc cagtatagtt ttaaaaatat gttttgtatt tagaagagaa ctgataaaat
    35281 ttggattttc acttgttagg gtatcaatga gtggaattat aaaaaatatc actgttccat
    35341 aaatcgaacc tgctttcaga ccaggataac gtaactgttt cttttctttt ttcatgagtt
    35401 tgctcctttt ttaaaaatct tcctcccaaa agagactgtt gaggtcagtt tggaggctgc
    35461 gggcgagatt gagacagagt tccaaggttg gattgtactt gtcgttttca atcatattga
    35521 tagtctgtct cgagacaccg atatccttgg cgagttcgag ctgggaaata cccaattcct
    35581 tgcgaaattc tttcacacga ttcatctggt ctcctttctg atttatgtcg tatatatttg
    35641 actatattat agtcttttaa acataaagtg tcaagtattt ttgacatatt ttttgaagaa
    35701 atagtagtct ccttgtccta tttgtctgac aagtgcaagc tggtcggatt tgtggtaaaa
    35761 tagataagat atgacaaaag aatttcatca tgtaacggtc ttactccacg aaacgattga
    35821 tatgcttgac gtaaagcctg aaggtatcta cgttgatgcg actttgggcg gagcaggaca
    35881 tagcgagtat ttattaagta aattaagtga aaaaggccat ctctatgcct ttgaccagga
    35941 tcagaatgcc attgacaatg cgcaaaaacg cttggcacct tacattgaga agggaatggt
    36001 gacctttatc aaggataact tccgtcattt acaggcacgt ttgcgcgaag ctggtgttca
    36061 ggaaattgat ggaatttgtt atgacttggg agtgtctagt cctcaattgg accagcgtga
    36121 gcgtggtttt tcttataaaa aggatgcgcc actggacatg cggatgaatc aggatgctag
    36181 tctgacagcc tatgaagtgg ttaatcatta tgactatcat gatttggttc gtattttctt
    36241 caaatacggt gaggataaat tctctaaaca gattgcgcgt aagattgagc aagcgcgtga
    36301 agtgaagccg attgagacaa cgactgagtt agcagagatt atcaagttgg tcaaacctgc
    36361 caaggaactc aagaagaagg gtcatcctgc taagcagatt ttccaggcta ttcgaattga
    36421 agtcaatgat gaactgggag cggcagatga gtccatccag caggctatgg atatgttggc
    36481 tctggatggt agaatttcag tgattacctt tcattcctta gaagaccgct tgaccaagca
    36541 attgttcaag gaagcttcaa cagttgaagt tccaaaaggc ttgcctttca tcccagatga
    36601 tctcaagccc aagatggaat tggtgtcccg taagccaatc ttgccaagtg cggaagagtt
    36661 agaagccaat aaccgctcgc actcagccaa gttgcgcgtg gtcagaaaaa ttcacaagta
    36721 agagggaaaa agatggcaga aaaaatggaa aaaacaggtc aaatactaca gatgcaactt
    36781 aaacggtttt cgcgtgtgga aaaagctttt tacttttcca ttgctgtaac cactcttatt
    36841 gtagccatta gtattatttt tatgcagacc aagctcttgc aagtgcagaa tgatttgaca
    36901 aaaatcaatg cgcagataga ggaaaagaaa accgaattgg acgatgccaa gcaagaggtc
    36961 aatgaactat tacgtgcaga acgtttgaaa gaaattgcca attcacacga tttgcaatta
    37021 aacaatgaaa atattagaat agcggagtaa gatatgaagt ggacaaaaag agtaatccgt
    37081 tatgcgacca aaaatcggaa atcgccggct gaaaacagac gcagagttgg aaaaagtctg
    37141 agtttattat ctgtctttgt ttttgccatt tttttagtca attttgcggt cattattggg
    37201 acaggcactc gctttggaac agatttagcg aaggaagcta agaaggttca tcaaaccacc
    37261 cgtacagttc ctgccaaacg tgggactatt tatgaccgaa atggagtccc gattgctgag
    37321 gatgcaacct cttataatgt ctatgcggtc attgatgaga actataagtc agcaacgggt
    37381 aagattcttt acgtagaaaa aacacaattt aacaaggttg cagaggtctt tcataagtat
    37441 ctggacatgg aagaatccta tgtaagagag caactctcgc aacctaatct caagcaagtt
    37501 tcctttggag caaagggaaa tgggattacc tatgccaata tgatgtctat caaaaaagaa
    37561 ttggaagctg cagaggtcaa ggggattgat tttacaacca gtcccaatcg tagttaccca
    37621 aacggacaat ttgcttctag ttttatcggc ctagctcagc tccatgaaaa tgaagatgga
    37681 agcaagagct tgctgggaac ctctggaatg gagagttcct tgaacagtat tcttgcaggg
    37741 acagacggca ttattaccta tgaaaaggat cgtctgggta atattgtacc cggaacagaa
    37801 caagtttccc aacgaacgat ggacggtaag gatgtttata caaccatttc cagccccctc
    37861 cagtccttta tggaaaccca gatggatgct tttcaagaga aggtaaaagg aaagtacatg
    37921 acagcgactt tggtcagtgc taaaacaggg gaaattctgg caacaacgca acgaccgacc
    37981 tttgatgcag atacaaaaga aggcattaca gaggactttg tttggcgtga tatcctttac
    38041 caaagtaact atgagccagg ttccactatg aaagtgatga tgttggctgc tgctattgat
    38101 aataatacct ttccaggagg agaagtcttt aatagtagtg agttaaaaat tgcagatgcc
    38161 acgattcgag attgggacgt taatgaagga ttgactggtg gcagaatgat gactttttct
    38221 caaggttttg cacactcaag taacgttggg atgaccctcc ttgagcaaaa gatgggagat
    38281 gctacctggc ttgattatct taatcgtttt aaatttggtg ttccgacccg tttcggtttg
    38341 acggatgagt atgctggtca gcttcctgcg gataatattg tcaacattgc gcaaagctca
    38401 tttggacaag ggatttcagt gacccagacg caaatgattc gtgcctttac agctattgct
    38461 aatgacggtg tcatgctgga gcctaaattt attagtgcca tttatgatcc aaatgatcaa
    38521 actgctcgga aatctcaaaa agaaattgtg ggaaatcctg tttctaaaga tgcagctagt
    38581 ctaactcgga ctaacatggt tttggtaggg acggatccgg tttatggaac catgtataac
    38641 cacagcacag gcaagccaac tgtaactgtt cctgggcaaa atgtagccct caagtctggt
    38701 acggctcaga ttgctgacga gaaaaatggt ggttatctag tcggggtaac cgactatatt
    38761 ttctcggctg tatcgatgag tccggctgaa aatcctgatt ttatcttgta tgtgacggtc
    38821 caacaacctg aacattattc aggtattcag ttgggagaat ttgccaatcc tatcttggag
    38881 cgggcttcag ctatgaaaga ctctctcaat cttcaaacaa cagctaaagc tttggagcaa
    38941 gtaagtcaac aaagtcctta tcctatgcct agtgtcaagg atatttcacc tggtgattta
    39001 gcagaagaat tgcgtcgcaa tcttgtacaa cccatcgttg tgggaacagg aacgaagatt
    39061 aaaaacagtt ctgctgaaga agggaagaat cttgccccga accagcaagt ccttatctta
    39121 tctgataaag tagaggaagt tccggatatg tatggttgga caaaggagac tgctgagacc
    39181 cttgctaagt ggctcaatat agaacttgaa tttcaaggtt cgggctctac tgtgcagaag
    39241 caagatgttc gtgctaacac agctatcaag gacattaaaa aaattacatt aactttagga
    39301 gactaatatg tttatttccc tcagtgctgg aattgtgaca tttttactaa ctttagtagg
    39361 aattccggcc tttatccaat tttatagaaa ggcgcaaatt acaggccagc agatgcatga
    39421 ggatgtcaaa cagcatcagg caaaagctgg gactcctaca atgggaggtt tggttttctt
    39481 gattacttct gttttggttg ctttcttttt cgccctattt agtagccaat tcagcaataa
    39541 tgtgggaatg attttgttca tcttggtctt gtatggcttg gtcggatttt tagatgactt
    39601 tctcaaggtc tttcgtaaaa tcaatgaggg gcttaatcct aagcaaaaat tagctcttca
    39661 gcttctaggt ggagttatct tctatctttt ctatgagcgc ggtggcgata tgctttctgt
    39721 ctttggttat caagtgcatc tagggatttt ctatattgtc ttcgctcttt tctggctagt
    39781 cggtttttca aacgcagtaa acttgacaga cggtgttgac ggtttagcta gtatttccgt
    39841 tgtgattagt ttgtctgcct atggagttat tgcctatgtg caaggtcaga tggatattct
    39901 tctagtgatt cttgccatga ttggtggttt gctcggtttc ttcatcttta accataagcc
    39961 tgccaaggtc tttatgggtg atgtgggaag tttggcccta ggtgggatgc tggcagctat
    40021 ctctatggct ctccaccaag aatggactct cttgattatc ggaattgtat atgtttttga
    40081 aacaacttct gttatgatgc aagtcagtta tttcaaactg acaggtggta aacgtatttt
    40141 ccgtatgacg cctgtacatc accattttga gcttggggga ttgtctggta aaggaaatcc
    40201 ttggagcgag tggaaggttg acttcttctt ttggggagtg ggacttctag caagtctcct
    40261 gaccctcgca attttatatt tgatgtaaga atggcaccct gatgtttcag ggtgtttttg
    40321 tgtttaaata cacaatgaaa atcaaagaac aaactagaaa gctaacttta ggctgctcaa
    40381 aatatagtag attgaaacta gaatagtaca catctacttc taaaatattg ttagaaatcg
    40441 atttgactgt cctgatcgat ttgtcctgtt cttatttcat tttaatatac agttttgagg
    40501 ttgtagataa ggagaagctg atgtggtttg aagaggtttt cgaagagtgt aaaaaaatat
    40561 tggtcagtta aagtcaaaac tcttgacctt ttctgactaa tgtagtatat tatgaacgta
    40621 aggtaaatga aagccttact agaacactta tttaaagtaa aatagaaaga ggtgggtcta
    40681 tgtttcatct agaaatcttc agaagtaaag atagtctact cctgcttgaa aaagaaaaac
    40741 cagaaatagt acgtagggta gcgatctagc tagtctaaat tttttaaaac aatggtcaaa
    40801 gatagtcaat atcagtaatc ataactaagt aaacaaaaag aggtaaagaa tatgaataac
    40861 aactttaata attttaataa catggatgat ttatttaacc aattgatggg tggtatgcga
    40921 ggatacagtt ctgaaaatcg ccgttactta attaatggac gcgaagtcac acctgaggaa
    40981 tttgctcact atcgtacgac tggtcaatta ccaggaaatg cagaaactga tgtgcaaatg
    41041 ccacaacagg catcaggtat gaaacaagac ggtgtccttg caaaactagg tcgaaacttg
    41101 acagcagaag cgcgtgaggg caagttggat cctgttatcg gacgaaacaa ggaaattcaa
    41161 gaaacatctg aaatcctctc acgccgcacc aagaacaatc ctgttttggt cggagatgca
    41221 ggtgttggta agacagcagt tgtcgaaggt ctagcgcaag ccattgtgaa cggagatgtt
    41281 cctgctgcta tcaagaacaa ggaaattatt tctattgata tctcaggtct tgaggctggt
    41341 actcaatacc gtggtagctt tgaagaaaat gtccaaaact tagtcaatga agtgaaagaa
    41401 gcagggaata ttatcctctt ctttgatgaa attcaccaaa ttcttggtgc tggtagcact
    41461 ggtggagaca gtggttctaa agggcttgcg gatattctca agccagctct ctctcgtgga
    41521 gaattgacag tgattggggc aacaactcaa gacgaatacc gtaacaccat cttgaagaat
    41581 gctgctcttg ctcgtcgttt caacgaagtg aaggtcaatg ctccttcagc agagaatact
    41641 tttaaaattc ttcaaggcat tcgtgacctc tatcaacaac accacaatgt catcttgcca
    41701 gacgaagtct tgaaagcagc ggtggattat tctgttcaat acattcctca acgtagcttg
    41761 ccagataagg ctattgacct tgtcgatgta acggctgctc acttggcggc tcaacatcca
    41821 gtaacagatg tgcatgctgt tgaacgagaa atcgaaacgg aaaaagacaa gcaagaaaaa
    41881 gcagttgaag cagaagattt tgaagcagct ctaaactata aaacacgcat tgcagaattg
    41941 gaaaagaaaa tcgaaaacca cacagaagat atgaaagtga ctgcaagtgt caacgatgtg
    42001 gctgaatctg tggaacgaat gacaggtatc ccagtatcgc aaatgggagc ttcagatatc
    42061 gaacgtttga aagatatggc tcatcgcttg caagacaagg tgattggtca agataaggcc
    42121 gtagaagctg tagctcgtgc tatccgtcgt aaccgtgctg gttttgatga aggaaatcgc
    42181 ccaatcggca gcttcctctt tgtagggtct actggggttg gtaagacgga gcttgctaag
    42241 caattggcac tcgatatgtt tggaacccag gatgcgatta tccgtttgga tatgtctgaa
    42301 tacagtgacc gcacagctgt ttctaagcta attggtacaa cagcaggcta tgtgggttat
    42361 gatgacaata gcaatacctt aacagaacgt gttcgtcgca atccatactc tatcattctc
    42421 ttggatgaaa ttgaaaaggc tgatcctcaa gttattaccc ttctcctcca agttctagat
    42481 gatggtcgtt tgacagatgg tcaaggaaat acagtaaact tcaagaacac tgtcattatt
    42541 gcgacctcaa atgctggatt tggctatgaa gccaacttga cagaagatgc ggataaacca
    42601 gaattgatgg accgtttgaa acccttcttc cgtccagaat tcctcaaccg ctttaatgca
    42661 gtcatcgagt tttcacactt gactaaggaa gacctttcta agattgtaga tttgatgttg
    42721 gctgaagtta accaaacctt ggctaagaaa gacattgact tggtagtcag tcaagcggct
    42781 aaagattata tcacagaaga aggttacgac gaagtcatgg gggttcgtcc tctccgtcgc
    42841 gtggttgaac aagaaattcg tgataaggtg acagacttcc acttggatca tttagatgct
    42901 aaacatctgg aagcagatat ggaagatggc gttttggtta ttcgtgagaa agcctaagac
    42961 agaattttga gaataaaaaa gaaggagcca gctgaaaaaa actggttcct tttgaggtac
    43021 gacgggcatg tcgtgtagta gagatgtact attctagttt caatctacta tagtagctca
    43081 gaaatcggta cttaaacgtg ctatatcaaa accagtcctt gaaaaacgtg gactggtttc
    43141 gtgtttggat tattactttg aacgacatgc gttaaaagtt agttgaaccg ccgtatgccg
    43201 aacggtacgt acggtggtgt gagaggggct agagattatc ccctactcga ttttaaatca
    43261 catgacgttc aaaggcatca tctgaaatcc cttgttccaa gatgagtttt gcccattctt
    43321 tagcagagaa gaggctgtgg tccttgtagt ttccgcaaga ttcgatggtt gtccctggga
    43381 catcttccca agtagtagtt tcagcgattt ccttgagcga atccttgata acagctgcga
    43441 ttttagcact ggtgtgacgt ccccacataa tcatgtggaa gcctgtgcgg caaccaaatg
    43501 gcgaacagtc aatcatgccg tcaatgcggg tacggatgag tttggctaag aggtgctcga
    43561 tagtgtgaag gccggcggta gggatagagt cttcgtttgg ttgcaccaag cgaatatcat
    43621 aattggagat gatgtctcct tttggtcctg tttcttcccc aatcaagcga acatagggtg
    43681 ctttgacaat ggtgtggtca agttcaaaac tttcgacaat aacttctttt gacatggtaa
    43741 atcctttcag ttttcttctc tcattatatc ataaaggttg ctcctgagac agagagaaaa
    43801 cctctccgag gctggagagg ttgaaatctt tacttacgat ataagcggtc gtattggtag
    43861 tatgggtcaa aggttacgtt gatacccagt ttacgaagga cattcttgtc ttcatcagtc
    43921 aagatgatgg ttgagtgggc ttcgcttcct ttgaggttgc cgagttcttc catagcgcgg
    43981 gtagcatcag gattttctgt agctgtgata gcaagtgcaa tcaggatttc atttgaatga
    44041 aggcgtggat tgcggctacc gagatgatcg attttaagac cttggattgg cttaacaact
    44101 tccggctcga ttaattttac ttctttagcg atgtcagctg attttttgat ggcgttgatc
    44161 aaggcagcgg ctgtagggcc aaagagttct gagttcttac cagtgacgat ttccccattt
    44221 ggcaattcaa aggctagggc tggtccacca gtttcttctg ctttttggcg cgcaacgaca
    44281 gcaaccttac ggtctgcagg tgtgataccg aggtcgttca tgagcaactc aattttcttg
    44341 acggcagctt cgccaacttt ttcagctttg aagtcaagaa ctgtttgata gtaacggcgg
    44401 atgatttctt gtttagaagc ttcgacagcg gcctcgtcat ctgtaatagc gaaaccaacc
    44461 atgttgacac ccatatctgt cggtgaagcg tatggtgatt ttccgagaat acgttccaac
    44521 atgcgtttga gcactgggaa gatttcgata tcacggttgt agttgacagt ggtttctcca
    44581 taggtttgga gatggaaggg gtcaatcatg ttgacatcat caaggtcagc tgtggcagct
    44641 tcataagcca agttaactgg atgatgaagg ggaagattcc aaacagggaa ggtttcaaat
    44701 ttagcgtagc cagatttgat gccattgatt tggtcgtggt acatattgga catacacgtt
    44761 gccaattttc cagaaccagg tccaggagcg gttacgacaa tcaagttgcg actggttttg
    44821 atgtagtcgt ttttgcccat gccttctggg gaaatgatgt gatccatatc cgtcggatat
    44881 cctttgattg gataatgaag atatgaatca attccgtttt tctcaagttg gttgcggaag
    44941 gcatctgcag cgggttggcc agcgtattgt gtaatgacaa cggaaccaac aaaaatcccc
    45001 aattcattga atttgtcaat caaacgaaga acttcttggt cataagaaat gcctaagtcg
    45061 ccacgtgctt tggaatgttc aatgttgcta gcattaatgg caatcacaac ctcaacctgc
    45121 tctttcaatt cttgcaagag cttgattttg ttgtcaggtt cataaccagg aaggacacga
    45181 gcagcgtgga aatcttctaa cattttaccg ccaaactcta agtagagctt gccgtcaaat
    45241 tggttaatac gctccaaaat atggtcgcgt tgtaaattca aatattgttc agaactaaaa
    45301 gcttgttttt tcattttttt acctctggac tctattataa taaaaaattg gaagttagga
    45361 aactacggag ctaaaaaaga aattaaaaag attaagcaaa cgcttgcaca aaattttaaa
    45421 aagtgctatc atagactata gattattaaa ataatgaggt aaacagatgc aagaaaaatg
    45481 gtggcataat gccgtagtct atcaagtcta tccaaagagt tttatggata gtaatggaga
    45541 tggagttggt gatttgccag gtattaccag taagttggac tatctagcca agctaggaat
    45601 cacagcaatt tggctttctc ccgtttatga cagccctatg gatgataatg gttacgatat
    45661 tgctgattat caagcgattg caacgatttt tggaaccatg gaggatatgg atcaactgat
    45721 tgcagaagct aagaagcgtg atatccgtat catcatggac ttggtggtca atcatacctc
    45781 ggatgagcat gcctggtttg tagaggcctg tgaaaatcct aatagccctg agcgagacta
    45841 ctatatctgg cgcgatgaac ccaatgacct agattctatc tttagtgggt ctgcttggga
    45901 atacgatgaa aagtcaggtc aatactatct ccactttttc agcaagaaac agccggatct
    45961 caactgggaa aatgaaaaac ttcgccagaa aatttatgag atgatgaact tctggattga
    46021 taaaggtatt ggtggtttcc gtatggatgt tattgacatg attggcaaaa ttcctgacga
    46081 gaaggtagtc aataatggtc ctatgctcca tccctatctc aaggaaatga atcaggcgac
    46141 ctttggagat aaggatctct tgacagtagg ggagacttgg ggagcaacgc cagagattgc
    46201 caagctctac tctgatccaa aggggcaaga attgtctatg gtcttccagt ttgaacatat
    46261 cggtcttcag tatcaggaag gtcagcctaa atggcactat caaaaagagc tgaatatcgc
    46321 taagttaaaa gaaatcttca acaaatggca gacagagtta ggagttgagg acggctggaa
    46381 ttccctcttc tggaacaacc atgacctccc tcgtattgtc tcaatatggg gaaatgacca
    46441 agaataccgc gaaaaatctg ccaaagcctt tgcaatcttg cttcatctta tgagaggaac
    46501 tccttatatc taccaaggtg aggagattgg gatgaccaac tatccgtttg aaacactaga
    46561 tcaagtagaa gatattgaat ctctcaacta tgcgcgtgag gctcttgaaa aaggtgttcc
    46621 gattgaagaa atcatggaca gtatccgtgt tattggacgt gacaatgccc gtacccctat
    46681 gcaatgggac gagagcaaaa acgctggttt ctcaacaggt caaccttggt tggcggttaa
    46741 tccaaattac gagatgatca acgtccaaga agcgctggca aatccagatt ctattttcta
    46801 tacctatcag aaactggtcc aaattcgcaa ggagaatagc tggctagttc gagctgactt
    46861 tgaattgctt gatacggctg ataaggtctt tgcttatata cgtaaggatg gcgaccgtcg
    46921 cttcctagtt gtggctaact tgtccaatga agagcaagac ttgacagtag aaggaaaagt
    46981 caaatctgtc ttgattgaaa acaccctagc tcaagaagtc tttgaaaaac aaatcttagt
    47041 tccatgggat gctttctgtg tggaaatgac tgactagaat gagcaaacct caagtttttg
    47101 aagcttgagg ttttttacta tagtggattg aaactagaat agtgcacctc tgcttctaaa
    47161 acattgttag aaatcgattt gactgtcccg atcgatttgt cctattctta tttcattttg
    47221 ctatacttgt agttgaggag aattaagctc ctcactagta aactcttcat aatccttttt
    47281 atttttatga agatattgtt tgaaagatgt gagtttccac ggatgggttt gtggagggat
    47341 atacttgcgt ctttcttttt ttgttctggt tcttgttcaa agtttttcga atagagttca
    47401 tgatttagta gctcctttgt gtgatagatt ttgtcagcga tattgaggta gatgtctccg
    47461 tcaaatgctt ttataactaa tgctttcgtc tttctgatga aatagacttc ttttccttgt
    47521 tcggtaggga tatagtaacg attttggaat cggatatggt gtccactatc gacgactctc
    47581 tccgccagtc tagccagaat gagattcctt tcagaggggt taggaacctc ctcgaagaca
    47641 gagagttttg tcttgtttcc aaactgttca ttaaaggttt gaatgtagga aagcaggaag
    47701 gtattggctt cttccaaggt atgaatattg ttttgttcca gttcgatagg caggcgagat
    47761 tgtagtgtct gattgagtct ttcgactctc cctttagctt gagggataga ggttgtctca
    47821 aggagaatcc ctagttggtg acaggcgtat ccaaactgtg tatgggtatc gtcctccatt
    47881 ttcttagagt tggaggcttg ataggtaaag accgttctct tatctgtttt aatttgaagg
    47941 ggaatgccgt gattggctaa gatttgttcg aggacatgat agtaagcatt caagttctct
    48001 tgtttatcaa aataagcgcc taggatattg ccagaagcat catcaatggc taagtgtaag
    48061 ttagatgttt gggctccaaa ctaggcatga gggctggcat ccatttgaat gagttcacca
    48121 gcaaatttct ttctgggtct actaggatgt acttttttag ggtcttcaag gaagttttca
    48181 gccgtcggta agattggatt gtctaggggt tgattcaggt tcagtttagc ttgttttctt
    48241 actctcttct ttgtctttct atgggactta ggcgacagga tatttttctt atagagtatt
    48301 tttctaacag tagtatcaga gagctgaatt ccttcttctt cagctagcaa ttcacagaaa
    48361 tgaaggacat ttggtttata tgtttcatag gagaggtatt tttttaggag acgttctttg
    48421 atttcatcag ggattgcatg ttttggtttt cgatttctgt ttccgtgtct gaaggcttct
    48481 tttcctttca gttgataggc tagtagcaga cgattgattt gtctttcaga aagattgagc
    48541 tcgacacagg ctcgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
    48601 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta ccaaatggga
    48661 cattttcacg ttcgatttac taaagacatt atcacattcg aattacacaa gatgcagata
    48721 gtgaaaataa aggtgtagac attaccgtaa aaaagtgata taattgtatg atgttcaatg
    48781 tataggtgtt aatcatgagt agacgtttta aaaaatcagg ttcacagaaa gtgaagcgaa
    48841 gtgttaatat cgttttgctg actatttatt tattgttagt ttgtttttta ttgttcttaa
    48901 tctttaagta caatatcctt gcttttagat atcttaatct agtggtaact gcgttagtcc
    48961 tactagttgc cttagtaggg ctactcttga ttatctataa aaaagctgaa aagtttacta
    49021 tttttctgtt ggtgttctct atccttgtca gctctgtgtc gctttttgca gtacagcagt
    49081 ttgttggact gaccaatcgt ttaaatgcga cttcgaatta ctcagaatat tcaatcagtg
    49141 tcgctgtttt agcagatagt gagatcgaaa atgttacgca actgacgagt gtgacagcac
    49201 cgactgggac tgataatgaa aatattcaaa aactactagc tgatatcaag tcaagtcaga
    49261 ataccgattt gacggtcaac cagagttcgt cttacttggc agcttacaag agtttgattg
    49321 caggggagac taaggccatt gtcctaaata gtgtctttga aaatatcatc gagtcagagt
    49381 atccagacta cgcatcgaag ataaaaaaga tttataccaa gggattcact aaaaaagtag
    49441 aagctcctaa gacgtctaag aatcagtctt tcaatatcta tgttagtgga attgacacct
    49501 atggtcctat tagttcggtg tcgcgatcag atgtcaatat cctgatgact gtcaatcgag
    49561 ataccaagaa aatcctcttg accacaacgc cacgtgatgc ctatgtacca atcgcagatg
    49621 gtggaaataa tcaaaaagat aaattaaccc atgcgggcat ttatggagtt gattcgtcca
    49681 ttcataccct agagaacctt tatggtgtag atattaatta ttatgttcgt ttgaatttta
    49741 cctcattttt gaaattgatt gacttattgg gaggggtaga tgttcataat gatcaagagt
    49801 tttcagctct acataggaag ttccttttcc cagtagggaa tgtccatcta gactctgagc
    49861 aggctctagg ttttgtacgt gaacgctact cactagccga tggagaccgt gaccgtggtc
    49921 gcaaccaaca aaaggtgatt gtggctattc ttcaaaaatt aacgtcaacc gaagcactga
    49981 aaaattatag tacgatcatt aatagcttgc aagattctat ccaaacaaat atgccacttg
    50041 agactatgat aaatttggtc aatgctcagt tagaaagtgg tggaacttac aaagtgaatt
    50101 ctcaagattt aaaagggaca gatcggatgg atcttccttc ttatgcaatg cctgacagta
    50161 acctctatgt gatggaaata gatgatagta gtttagctgt agttaaagca gctatacagg
    50221 atgtgatgga gggtagatga aatgatagac atccattcgc atatcgtttt tgatgtagat
    50281 gacggtccca agtcaataga ggaaagcaag gcacttttaa gagaagctta taatcaagga
    50341 gttcgaatga ttgtgtctac ttcgcatcgt cgaaaaggga tgtttgaaac tccggaagag
    50401 aagatagcag aaaactttct tcaggttcgg gaaattgcaa aagaagtagc agatgattta
    50461 gtcatagctt atggcgcaga gatatactat actctggatg ctctagaaaa gctagaaaaa
    50521 aaagaaattc ctacccttaa tgatagtcgt tatgctttga ttgagtttag catgcatact
    50581 tcctatcgtg agattcatgc gggattgagc aatattttga tgttgggaat cacgccagta
    50641 attgctcata ttgaacgtta tgatgcctta gagaataacg aaaaacgtgt tcgtgaactg
    50701 attgatatgg ggtgctatac tcagataaat agttatcatg ttttaaaacc taagttcttt
    50761 ggtgaaaaat ataaattcat gaaaaagaga gctcggtatt ttttggaacg tgatttagtt
    50821 catgtagttg caagtgacat gcacaattta gacagtagac ctccatatat gcaacaggca
    50881 tatgatatca ttgctaagaa atatggagcg aaaaaagcga aagaattttt tgtagataat
    50941 cccagaaaaa ttataatgga tcaattaatt taggagaaaa tatgaaggaa caaaacactt
    51001 tggaaatcga tgtattgcaa ctattcaggg ctttatggaa aagaaagttg gtcattttat
    51061 tagtggcaat tataacttct tcagttgctt ttgcctacag tacttttgtt atcaaacctg
    51121 agtttactag tacgactcgg atttatgtag ttaaccgtaa tcaggaagag aagtctggtt
    51181 taaccaatca agacttgcag gcaggatcat acttggttaa agactatcgt gaaattatcc
    51241 tatcgcagga tgttttggag gaagttattt ctgatttgaa actagatttg acgccaaaag
    51301 gtttggctaa taaaattaaa gtaacagtac cagttgatac ccgtattgtc tctgtctcag
    51361 ttaatgatcg agttcctgaa gaggcaagcc gtatcgctaa ctctttgaga gaagtagctg
    51421 ctcaaaaaat tatcagtatt actcgtgttt ctgatgtgac aacactggag gaggcaagac
    51481 cggcgatatc accatcttcg ccaaatatta aacgcaatac actaattggt tttttggcag
    51541 gggggattgg aactagtgtt atagttcttc ttcttgaact tttggacact catgtgaaac
    51601 gtccggaaga tatcgaagat acactgcaga tgacactttt gggagttgta ccaaacttga
    51661 gtaagttgaa ataggagaga ggaatgccga cattagaaat agcacaaaaa aaactggagt
    51721 tcattaagaa ggcagaagaa tattacaatg ccttgtgtac aaatatacag ttgagcggag
    51781 ataaactaaa agtaatttcc gttacttctg ttaaccctgg ggaaggaaaa acaactactt
    51841 ccgtaaatat agcaaggtcg tttgcgcgta caggctataa aactcttttg atcgatggcg
    51901 atactcgaaa ttcagttata tcaggagttt ttaaatcgcg tgaaaaaatt acagggctaa
    51961 cagaattttt atctgggaca gctgatttat ctcacggttt atgtgataca aatattgaaa
    52021 atttatttgt aattcaatcg ggatctgtat caccaaaccc tacagccttg ttacaaagta
    52081 aaaattttaa tgatatgatt gaaacattgc gtaaatattt tgattatatc attgttgata
    52141 cagcacctat tggaattgtt attgatgcgg caattatcac tcaaaagtgt gatgcgtcca
    52201 tcttggtaac agcaataggt gaggtgaata aacgtgatgt ccaaaaagcg aaacaacaat
    52261 tagaacaaac agagaaactg ttcctaggag ttgttttaaa taaattggat atctcggttg
    52321 ataagtatgg agtttacggt tcctatggaa attatggtaa aaaataactt aggaaagatt
    52381 ttatggatga aaaaggattg aaaatttttc tagcagtaat acagagtatt attgtcattt
    52441 tattggttta ttttcttagc tttgttagag agacagaact tgaacgttct tcgatggtta
    52501 tactatacct tctccacttt tttgtattct attttagttc ctatggtaac aattttttta
    52561 aaagagggta cctagttgag tttaatagta ctataagata tatttttttc tttgcaatag
    52621 ctataagtgt attaaacttt tttatagcgg aacggtttag tatctctaga agaggaatgg
    52681 tatacttctt aactttagaa ggaatatcct tatacttgtt aaatttctta gtaaagaaat
    52741 attggaagca tgtgtttttt aatctaaaaa atagcaagaa aattttactg ttaacagtaa
    52801 cgaaaaatat ggaaaaagtt cttgataaat tgctagaatc tgatgaactt tcatggaaat
    52861 tggtagcagt aagtgttttg gataaatctg attttcaaca tgataaaata cctgtaattg
    52921 aaaaggaaaa aattattgaa tttgcaacgc atgaagttgt ggatgaggtg tttgtcaatc
    52981 ttccaggaga gagctacgat attggagaaa ttatctctag gtttgagaca atggggatag
    53041 atgtaactgt aaatcttaaa gcatttgata agaatttggg tcgcaataaa caaattcatg
    53101 agatggtagg attgaatgta gtcactttct ctacaaattt ttataaaact agtcatgtga
    53161 tttcaaagag aattctcgat atttgtggtg ccactattgg ccttattctt tttgctatag
    53221 ctagtctagt tttagttcca ttgattcgta aagatggcgg atcagctatt tttgctcaaa
    53281 ctcgtatagg gaaaaatggt cgacatttta ccttttataa attccgttcg atgcgaatcg
    53341 atgctgaagc tatcaaagaa cagttgatgg atcaaaatac gatgcaaggt ggtatgttta
    53401 agatagacaa tgatcctcgt gttacaaaaa ttggtcgctt tattcgtaaa actagtttag
    53461 atgaattgcc gcagttttgg aatgtcttta taggagatat gagtttggtg ggaacacgtc
    53521 cacctacagt agacgagtat gatcagtata ctccagaaca gaaacgtcga ctcagcttta
    53581 aacctggtat tacaggttta tggcaggtta gcggccgtag taaaataacc gattttgacg
    53641 atgttgtaaa attagatgtg gcttatattg ataattggac aatctggaaa gatattgaaa
    53701 ttttgcttaa aactgttaaa gttgtattta tgagagatgg agcgaagtaa ttcatatgaa
    53761 tgagagaatg caaatattag gagttactat agatccatta acgatgaaag aaacagtgga
    53821 tgctgttgaa cagtatgttt taaagaagca tcctttgcac ttgatgggag ttaacgcaga
    53881 taaaattaat cagtgccatg aagatgagaa aatcaaaaaa atcgttaatg aatcaggaat
    53941 cattaatgcg gatggagcat cagttgttct tgcaagtaag tttttaggaa cgcctgttcc
    54001 tgaacgagta gcgggtattg atttgatgca atgtctttta gagttgtcaa ataaaaaagg
    54061 atattcagtt tacttttttg gagctaaaga agaaatttta caagatatgc taaaagtttt
    54121 taaacaaaga tatcctaatc ttaatgttgt tggctataga aacggatatt tttcacctga
    54181 agatgaaaaa aagattcagg aagatattaa aggaggaaaa ccagattttg tatttgtagg
    54241 gattacttct cctaaaaagg agtatatcat ccaatcattc atggataatg ggattaatgc
    54301 tgtctttatg ggtgttggtg gcagttttga tgtattatca ggacacatta aacgagctcc
    54361 attatggatg caaaaattga atctggaatg gttgttccgt gtagttaatg aacccaaacg
    54421 cctttttaag cgttatttta tagggaatgt tacttttatt aagcgagttt tagatgaaaa
    54481 gcgaaaatca aaaaaataat attctacata tttctcgtac aatggatatt ggtggtgcag
    54541 aacgtattgt atatcaattg agttctgatt tgaaagatga atttgatagt gtccatgtcg
    54601 cttcaacagg tggactatgg gagagcaaac ttttatcaaa aggagttcaa catcataaaa
    54661 ttttagatgt tgacagtaag aatcccctaa ctatggtaaa gatcctttca agtttttata
    54721 gaattatcaa aaataatgat attaccattg ttcacactca tcatcggatg gctgcctttt
    54781 atattcgctt gttgaaaatc attaatccta agttgataca tgtttacact gcacataatg
    54841 tatttaaaaa taaattacca ttatacaaat tttccttaaa aaaagcgtat tgtgtagctg
    54901 taagtaaagc ggttaatgat aatttgaaac atgatgtagg aatcagtgat agtacagtaa
    54961 tctataatgg ggttgttttt gaaaaaactg ataagctagt tggtgaaatt gtaaactata
    55021 acggaatcaa attggggtgt attgctaggt tatcagagca aaaaggattg acctacctaa
    55081 ttgatgcgat gtctttagtg aaacacagga atattacgtt gttcattgtc ggagatggag
    55141 agcttcgaga tgagcttgaa aacaaggtga aacaattaaa tcttcaaaaa tcagtgatat
    55201 ttttaggtta tcgaaaagat attgttgaat gtatcaatag ttttgattac ttagtgtcat
    55261 catctttata tgaggggttt ggtctaaatg caattgaagc atttatgaag gggaaaacga
    55321 tggtttcatc agatatccca ggaataaatg aagtagtcaa taacaaaaat gggattcttg
    55381 ttcctgttaa ggatgatgtt gccttagcga gagcgattga aaagcttgcg actgacaaaa
    55441 aacttagaga aaaactcgct tatcaagcaa aaaaagatta tgagacaaaa tttaattatt
    55501 ccttgttttt ggataattat cggagacttt atagaaaatt aatgggagaa tcaaaatgaa
    55561 aaaagtcatg ttggtttttg ggacacgtcc agaagctatt aaaatgtgtc ctttagtaaa
    55621 tgaactgaaa caaaatgatt caatcaagac acttgtgtgt gtaactggtc aacataagga
    55681 aatgttagag caggttttag aggtgttcaa ggttgtccca gattatgatt tgggtatcat
    55741 gaaagcaaat cagaccctat ttaccattac gactagtatc ttggataaaa ttcaggcggt
    55801 tctagagaaa gaaaaaccag atcttgttct tgttcatgga gatactacaa caacatttgc
    55861 gacagcttta gcagcatttt atatgggaat caaagtggga cacgtcgaag caggtctcag
    55921 aacctataat ctccaaagtc catttccaga ggaattcaat cgccagacaa cttctatcat
    55981 tgccgactat cattttgcgc cgactgaagt tgcgaaggaa aatctactaa gagaggacag
    56041 aaaaaatatc tttgtgacgg gaaatacagt cattgatgct ttgaagacga ctgttcaaga
    56101 taactaccat cacccaattt tagaatgggc aaagggtagt aagttaatca tgctaacggc
    56161 tcatcgacga gaaaatcttg ggaaaccgat ggaaaacatg tttaatgctg ttaatcgaat
    56221 tttaaacgaa ttcgaagatg taaaagttgt atatcccatc cataaaaatc ctaaggttcg
    56281 agaattagca agtaaagttt ttggcgataa tgaacgaatg aaaatcattg aaccgttaga
    56341 ggttattgat tttcataatt ttatgaatca aagctatatg attttgacag attctggagg
    56401 tgtacaggaa gaagcacctt ctttaggaaa acctgtcttg gtgatgcgtg atacaacaga
    56461 gcgtcctgag ggggtcgttg ctggcacgtt gaagttggta ggaacagaag aagagaatat
    56521 ctatagcaac ttcaaacttc ttcttgaaga tgaaacagaa tataacaaga tgagcaaagc
    56581 tagcaaccct tatggagatg gaatgacttg taaacggatt gttgatatta ttctggaagt
    56641 ataaaaataa ttcaattatg gatgaggaaa gaataaatta gattgatgat aaaaagaaaa
    56701 atatatataa tctgtgtatt actgtcgtat tttttaccta gtttttttac agttggatca
    56761 atggcgactt tatttgcttt gctttctact ttagttaagc tatttgtaac ttttgattat
    56821 attcccaaag ttcttttaca gcataaagta acactgattg atattctagt tgttctattt
    56881 ttattttttc aagcatttgc agcttttcaa agccaaacgt tgtaccttaa ctatgttgga
    56941 ggtcagtttt tccttttagg actatattca tttttgaaac actttttatt gttggattgt
    57001 aaaacaacaa taaaaagcct ctttttaacc tttatcttgt ttttgtgtgt tcaagtaata
    57061 actcaattgc tttttccggt cggtttcgat tctttgcacc ctacaggaga taatagactt
    57121 tactttttag gaagaaagaa catagctacc ccgtatatca ttgttggatt aggtggtttt
    57181 tacttattaa ataaaaaaat gaatgagttt atatcactaa aggagattat ttttttagga
    57241 ttgttcggaa ttttatcatt tttaacacaa tccagtacag cgattatatg ttatgggctt
    57301 tttattttca tacgtctatt gggattaaag gaaaatatag gaaaactata ttctttagtc
    57361 agtatggctg tatatgtttg tttttctctt tctataatct tttcacaaag tacaatttta
    57421 tcaactttta cagcgatttt ttccaaaaat gcaacttttt caggacgtat caatatttgg
    57481 caattagcga ttcgtatttt tgaagagaat ttttggtttg gacgaggcct caacgttaat
    57541 tttaatgctt ggacaaatgg gattatcgtt aattcagcac acaatacctt attagatata
    57601 cttgctcgaa ctggtatatt tccaggtgtt ttatttatag tgcttcttat gaatctgttt
    57661 cttggaaaat atagaataga atcaaaaaca ctgttggcta tgttagcctc atttatgatt
    57721 tatattacaa tggagacttc atctatgagc atattgctgc taattattgt tctttgtgta
    57781 tattggccac gtggagagga gaagttgcat gagcaagtta cttaatacca tctattatcg
    57841 actatttaga ttatataaaa aaatttatct acaagttttt agtaaaaaaa cggttgacat
    57901 cttgggtgaa aaggctaatt tagaaaatga actaattgtt tcgtttacaa caattccaag
    57961 tcgtcttaat tatttaccaa gtatgattaa aagtattttc aatcagacaa ttatccctaa
    58021 ccgttttatt atgtatgttt ataaagatga atttgaaggc attaatttgg agagtattct
    58081 agaacttgaa atcaaaaatg gtttggaaat tgtctatgtg gatgagaatc ttcgttcaca
    58141 caaaaaatat ttttatgcta tgaaggataa tccgaacagt atagttgttc tagttgatga
    58201 cgatatcatt tatcctcgaa atactataaa gaaactgatt gcatcctata gaatttatcc
    58261 acagtgtgtt tcagcaatgc gatgtcatcg aattaagttg ttttctgatg gaagtttata
    58321 tccatataat caatgggaat atgaaatatc aggagctact ataccatcgt attttaattt
    58381 ttttactagc ggaggtggga cattatttcc tccatgtact cgcaatgagg atctttttaa
    58441 taaaaaaaat atcagggaac tcagttttct agctgatgat gtatggttga attttttggt
    58501 agtcaaaaat ggaattaaaa cagtaaaagc aacacgatat aaagggacac cattaactat
    58561 agatgacaat cctgaggaga gtttggtata tttgaatacc gtatatgata acaacaatga
    58621 taaatgtatt agaaatatgg ttgaatatta tcaaattaat tttacagagg ataaataatg
    58681 aatgttgaaa aaaatttgaa acgtggaatt ttctatactg caatagggaa gtattcgaat
    58741 gtagttattc aattacttgt aacagcaatt cttagccgga ttctctcacc tgtagaatat
    58801 ggtatcgtag cagtagtaaa tgtatttctt ttattttttc aaatgttagc agattcagga
    58861 ataggacctg ctattgttca aaataaagaa ctgaataagt cagatttaat cagcattttc
    58921 tcattaacga tttatagtgg aatcgtactt tcttgtattt ttgctttact aggatatcct
    58981 atgggggtgt tatacggcga aactatttat gttagtttat ttccgctttt aggtctttgt
    59041 gttttatttt ataccattac aattgtaccg caagctattc taatgaaaac gatgaacttt
    59101 aaaatagtta attttctaac tattttttct aacattgcta gtggcttggt gggcgttatt
    59161 ctagcggtat cacattttgg tgtctattct ctaatttttt ctaatattgt aaaagcaatg
    59221 gttttattta tcgctttata ttctaaagca aagataaatt tctattggaa agtttcaaaa
    59281 agttctattg gtaaaatttt tgagttttca aaattccaat ttttgtttaa tttttggaat
    59341 tattttgcta gaaatttaga taatttatta attggacgat tgattagccc gtcagatctt
    59401 ggttattatg ataaggctta tcaattatct ttgtacccta atcaaatttt atcacaggtt
    59461 gtgacaccag cattgcatcc tattatgtcc aattttcaag ataatatcag taagataggc
    59521 gaagtttatc ttcagatttc tagaatattc gtaatagttg gcatacctat ttcggcttat
    59581 ttatacttta atgctcaata tgttgttaca tttatgtttg gagataattg gtcccaaagc
    59641 gttccggtgt ttcaaatatt agcagcaaca atttggttac aaatggctaa tagccctact
    59701 ggtgcatttt accaagcaac taatcagaca aagttattgt ttagaattgg cttattgaca
    59761 tcttttatta atattttagc tattgttatt ggtgtaatgt tgcaaagcat ccaatgtgtt
    59821 gcgtacatgc ttttgatttc tttcagtctt agtttagtaa tcaatactct ttacttaaca
    59881 aaaaaagtat taaatatttc tgctcaaaaa tatataaaac cgatattaat taatttgaca
    59941 attatcagtc cctatattgt gtttaatttg tttatctcgg attttgtaaa cgaccttatt
    60001 ttacatttat cagttcagtt tgttatttta tttttaatat ggggaattgg gatgtatatt
    60061 agcggagagt atcgacgagt cttcgcggtt ataagaaaat aaaggagcaa ttttgtgaaa
    60121 attttatttg tgaatatgtt ttataatgaa gcaggaggtg cacaaaagag caccaaattt
    60181 ctagctgaac aacttgtaaa agaaggacat gaggtatttg catactctga tgatgccatt
    60241 gattcagata ttgatgaagt tattaatagg gttaagattt ttagaagaag aaccccgctt
    60301 tttagtcttc actatatttt cagtgctaag aagaatccag ctaaacattt tttctataaa
    60361 atatttgaaa cctataatgt atttgcgaaa agaaaattta tcaatatcat tgaggaagta
    60421 caaccagata ttgttcactt taatacaatt tcaggaatgt ctttatcaat agttaaagaa
    60481 gctaaaaaga gaaatatcag gaccgtttgg accttgcgag actattggct agaatatcca
    60541 tggggtgaga aagatatttc tcttatcaaa cttctaaata gaatatatag accttttgtt
    60601 aagaaaagtt taagacactt agacatagta acagcaccat cagcatttac tttgaataat
    60661 tttatcaata aaggatttat taacccatct tctcaaaaat gtatggtcat tgaaaatgct
    60721 gttatatttt caaagggtaa gttggaaaat attatcaaac aaaagcaaga aacaaaaaga
    60781 aatccagaaa aaacgaattt tctttttgta ggttctatac ttgaaataaa gggcgttttt
    60841 aacttagtaa aggcctttaa aaaactaact agtccggaaa tttctttgaa aattgtagga
    60901 aaaggaaaag atttagaaaa acttcaaaaa gaaattgaga gtgatcctag aatacaatat
    60961 ttaggctttc aagatacaga aaccctctat aaaacttatc aaaattcgga tgtattggtc
    61021 gtcccatctg cttgggatga accgtttggt agagttgtaa ttgaaggaaa tgcaaatggc
    61081 cttcctgtca tcacttcaga tagaggtgga ttacctgaaa ttgtgcaaac agtaggagga
    61141 ggagaaattt ttacagatga atcgggaaat acattatata gtcttttaaa gaaatttgta
    61201 gaaatggaaa actacaatgt ctattaccaa tgcattcttg agaatattga tatttattct
    61261 atcgaaaaac aaagtgaaaa gttcactaat ctgtatttag ataaagaaat cgaataataa
    61321 gttgtttttg tagcagtaac ggaggtaatt atgaaaatag cggtagcagg aacaggttat
    61381 gtaggattat caattgctgt gttgttggca caacatcacg aaataaaagc agttgatatt
    61441 gttgctgaaa aagttgaatt aatcaatcaa aaaaagtctc caattcagga tgattatatt
    61501 gaaaaatatt tagtggaaaa agatttaaat ttggaagcaa ctatggatgc taagtatgct
    61561 tatgaagatg cagaatttgt tgttgttgca gcaccaacaa attatgattc gaaaaagaac
    61621 ttttttgata catcagcagt agaggcggtc attcaacaag taaaaaaaat taatcccaaa
    61681 gcggtaataa ttattaaatc tacaatccct gttggattca cagagtccgt tcaaaagaaa
    61741 tatcagtata aaaatatctt atttagtcca gaatttttac gcgaatctaa agcattatac
    61801 gataacctgt atccaagtcg catcattgtt ggaactgact tagaagatac ttatttaaaa
    61861 gacaaagcta atatatttgc ttccctacta aaagaggggg ctattaaaga agacatagat
    61921 acacttttca tgggagtaac ggaagctgag gcagttaaat tatttgccaa tacctacctt
    61981 gcgttgcgtg tttcttactt taatgaattg gatacttatg cagaggtgaa aggtttggat
    62041 gcaaaagcca ttattgatgg tattggccta gatccacgta taggaaatca ttataacaat
    62101 ccatcctttg gctacggtgg gtattgttta cctaaagata gtaagcagtt attagcaaat
    62161 tactttgatg taccacaaaa tatgatgaca gcaactgttg aaagcaatcg aacgcgtaag
    62221 gattttatcg ctcaacggat tttagaaaaa gctggagtat ctgagactga tagtttagat
    62281 gcgtttaaga acatacaaga tattgttata ggtatttatc gtttaacaat gaaaagtaat
    62341 tcggataact tccgtcactc ctctattcaa ggcgttatga aacgattgaa agctaaggga
    62401 gttacggtta tcatctatga gccaactttg aaggacgggg aaacattctt tggtaataaa
    62461 gtggtaaata atctagataa atttaaagaa gctagtaatg ttattgtagc caatcgtttt
    62521 gaaccttcac tagaggatgt gtctaataaa gtttatagtc gagatatatt taagcgtgac
    62581 taataaatac gtccacgtga cgtaatatct attaaatgaa taaaatatta ataaacatgg
    62641 catcgatatc ttttttggac ttgaattgtc aaattaagtg cacatttttt tcatagaaaa
    62701 cttcataagg tgttttccgg ttaagacatt ttcgtggtct attatctaag ttggtttata
    62761 taatagactt cctgcgaaac aaaatatggt ataatagttc tatgaattat gaagcaagta
    62821 aacaactaac cgatgtacga tttaagcgtc ttgttggtgt tcaacgcacg acttttgaag
    62881 agatgttagc tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac
    62941 ctaaattaag cctagaagac tttcttatgg cgactcttca ataagtgcga gaataccgca
    63001 cttatgaaca aattgcggct gattttggta ttcacgaa
//
LOCUS       DBERYS010000007        60964 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.007, whole genome shotgun sequence.
ACCESSION   DBERYS010000007 DBERYS010000000
VERSION     DBERYS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 60964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.007"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..463)
                     /locus_tag="ACL8LA_000506"
     CDS             complement(<1..463)
                     /locus_tag="ACL8LA_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812319.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
     gene            complement(453..1448)
                     /locus_tag="ACL8LA_000507"
     CDS             complement(453..1448)
                     /locus_tag="ACL8LA_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812320.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(1856..2281)
                     /locus_tag="ACL8LA_000508"
     CDS             complement(1856..2281)
                     /locus_tag="ACL8LA_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812321.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2425..2769)
                     /locus_tag="ACL8LA_000509"
     CDS             complement(2425..2769)
                     /locus_tag="ACL8LA_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGS1812322.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKK"
     gene            complement(2815..3021)
                     /locus_tag="ACL8LA_000510"
     CDS             complement(2815..3021)
                     /locus_tag="ACL8LA_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812323.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(3008..3169)
                     /locus_tag="ACL8LA_000511"
     CDS             complement(3008..3169)
                     /locus_tag="ACL8LA_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1812324.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3528..4484)
                     /gene="lytA"
                     /locus_tag="ACL8LA_000512"
     CDS             complement(3528..4484)
                     /gene="lytA"
                     /locus_tag="ACL8LA_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS1812325.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(4860..6230)
                     /locus_tag="ACL8LA_000513"
     CDS             complement(4860..6230)
                     /locus_tag="ACL8LA_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS1812326.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6537..7703)
                     /gene="recA"
                     /locus_tag="ACL8LA_000514"
     CDS             complement(6537..7703)
                     /gene="recA"
                     /locus_tag="ACL8LA_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGS1812327.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(7758..9014)
                     /locus_tag="ACL8LA_000515"
     CDS             complement(7758..9014)
                     /locus_tag="ACL8LA_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGS1812328.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(9099..10115)
                     /locus_tag="ACL8LA_000516"
     CDS             complement(9099..10115)
                     /locus_tag="ACL8LA_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGS1812329.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(10123..10641)
                     /locus_tag="ACL8LA_000517"
     CDS             complement(10123..10641)
                     /locus_tag="ACL8LA_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS1812330.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGELFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(10631..11074)
                     /gene="tsaE"
                     /locus_tag="ACL8LA_000518"
     CDS             complement(10631..11074)
                     /gene="tsaE"
                     /locus_tag="ACL8LA_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGS1812331.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(11160..11780)
                     /locus_tag="ACL8LA_000519"
     CDS             complement(11160..11780)
                     /locus_tag="ACL8LA_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812332.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(12163..13026)
                     /locus_tag="ACL8LA_000520"
     CDS             complement(12163..13026)
                     /locus_tag="ACL8LA_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1812333.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            13218..13340
                     /locus_tag="ACL8LA_000521"
                     /pseudo
     CDS             13218..13340
                     /locus_tag="ACL8LA_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
     gene            13491..13715
                     /gene="pneA1"
                     /locus_tag="ACL8LA_000522"
     CDS             13491..13715
                     /gene="pneA1"
                     /locus_tag="ACL8LA_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HGS1812334.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            13722..13910
                     /gene="pneA2"
                     /locus_tag="ACL8LA_000523"
     CDS             13722..13910
                     /gene="pneA2"
                     /locus_tag="ACL8LA_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HGS1812335.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            14145..17015
                     /gene="lanM"
                     /locus_tag="ACL8LA_000524"
     CDS             14145..17015
                     /gene="lanM"
                     /locus_tag="ACL8LA_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="HGS1812336.1"
                     /translation="MSIISEKTFEEMLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAK
                     NSEWYKFAEGSFEIFRKHPQRINFKKDFSRILEPFIINLLKLINNYLENKEYEWIDKN
                     GNIFSSLVFYLEDLIYPWIVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENI
                     LNFYNKYPVLLRQISESCLRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGD
                     THSQGKTVLILFFDDAKIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDH
                     SYEEFIDYLPLEQKKKLPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFET
                     MLQQPLFDDKTGQSLLDTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNG
                     QVLINLNTDKVKFDIGKIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNK
                     TEEFKMLLKANLYGLKTRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKF
                     SNQGIVASECEQMRLLDIPIFYTDTNINEIYDDFGNHFISLHQFSGYDYFMKYLPNNN
                     LENLEKQLDIIEIYFPSFDQRKKGYKLNLSNRKKLPNESLVTILHNEVENIADSLVIN
                     NIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFMYYVKLLKDKSSYTQLYNK
                     MLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLACLNYIKSVLTELEKTDFA
                     SVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLGDTLYNDVSERDNSNFGYS
                     FGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIHSLKWCSGEFGTLVNHPQS
                     VPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIV
                     QTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLLEI"
     gene            17365..18069
                     /locus_tag="ACL8LA_000525"
     CDS             17365..18069
                     /locus_tag="ACL8LA_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGS1812337.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            18050..18979
                     /locus_tag="ACL8LA_000526"
     CDS             18050..18979
                     /locus_tag="ACL8LA_000526"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HGS1812338.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            18976..21084
                     /locus_tag="ACL8LA_000527"
     CDS             18976..21084
                     /locus_tag="ACL8LA_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGS1812339.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLMYLLRQIYVAS
                     LKYIMDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILSNNFIAAILDL
                     LMIVVYAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIKEKVNVQNITS
                     EVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLT
                     LIVGVNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNT
                     KSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSG
                     KSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTF
                     GHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKP
                     SVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEI
                     GTHRELLELGGIYKQLYSNN"
     gene            21108..22847
                     /locus_tag="ACL8LA_000528"
     CDS             21108..22847
                     /locus_tag="ACL8LA_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS1812340.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            22898..23665
                     /locus_tag="ACL8LA_000529"
     CDS             22898..23665
                     /locus_tag="ACL8LA_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGS1812341.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            23667..24299
                     /locus_tag="ACL8LA_000530"
     CDS             23667..24299
                     /locus_tag="ACL8LA_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812342.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(25228..25641)
                     /gene="ndk"
                     /locus_tag="ACL8LA_000531"
     CDS             complement(25228..25641)
                     /gene="ndk"
                     /locus_tag="ACL8LA_000531"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGS1812343.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(25752..29429)
                     /gene="rpoC"
                     /locus_tag="ACL8LA_000532"
     CDS             complement(25752..29429)
                     /gene="rpoC"
                     /locus_tag="ACL8LA_000532"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGS1812344.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(29462..33073)
                     /gene="rpoB"
                     /locus_tag="ACL8LA_000533"
     CDS             complement(29462..33073)
                     /gene="rpoB"
                     /locus_tag="ACL8LA_000533"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS1812345.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            33489..33559
                     /locus_tag="ACL8LA_000534"
     tRNA            33489..33559
                     /locus_tag="ACL8LA_000534"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33521..33523,aa:Cys,seq:gca)
     gene            complement(33757..35088)
                     /locus_tag="ACL8LA_000535"
     CDS             complement(33757..35088)
                     /locus_tag="ACL8LA_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGS1812346.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRIAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(35317..36141)
                     /gene="endA"
                     /locus_tag="ACL8LA_000536"
     CDS             complement(35317..36141)
                     /gene="endA"
                     /locus_tag="ACL8LA_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGS1812347.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(36180..36368)
                     /locus_tag="ACL8LA_000537"
     CDS             complement(36180..36368)
                     /locus_tag="ACL8LA_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS1812348.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(36361..37644)
                     /gene="murA"
                     /locus_tag="ACL8LA_000538"
     CDS             complement(36361..37644)
                     /gene="murA"
                     /locus_tag="ACL8LA_000538"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS1812349.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(37668..37898)
                     /locus_tag="ACL8LA_000539"
     CDS             complement(37668..37898)
                     /locus_tag="ACL8LA_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGS1812350.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(37956..38993)
                     /locus_tag="ACL8LA_000540"
     CDS             complement(37956..38993)
                     /locus_tag="ACL8LA_000540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGS1812351.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(38977..39465)
                     /gene="coaD"
                     /locus_tag="ACL8LA_000541"
     CDS             complement(38977..39465)
                     /gene="coaD"
                     /locus_tag="ACL8LA_000541"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGS1812352.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGRDIACYVPESILEEIRNEK
                     KD"
     gene            complement(39455..39994)
                     /gene="rsmD"
                     /locus_tag="ACL8LA_000542"
     CDS             complement(39455..39994)
                     /gene="rsmD"
                     /locus_tag="ACL8LA_000542"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGS1812353.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(40059..41051)
                     /gene="asnA"
                     /locus_tag="ACL8LA_000543"
     CDS             complement(40059..41051)
                     /gene="asnA"
                     /locus_tag="ACL8LA_000543"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGS1812354.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(41164..41847)
                     /locus_tag="ACL8LA_000544"
     CDS             complement(41164..41847)
                     /locus_tag="ACL8LA_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HGS1812355.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(41903..42643)
                     /locus_tag="ACL8LA_000545"
     CDS             complement(41903..42643)
                     /locus_tag="ACL8LA_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGS1812356.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            42677..42955
                     /locus_tag="ACL8LA_000546"
     CDS             42677..42955
                     /locus_tag="ACL8LA_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGS1812357.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            43033..43959
                     /gene="yidC"
                     /locus_tag="ACL8LA_000547"
     CDS             43033..43959
                     /gene="yidC"
                     /locus_tag="ACL8LA_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS1812358.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(44034..44828)
                     /gene="pflA"
                     /locus_tag="ACL8LA_000548"
     CDS             complement(44034..44828)
                     /gene="pflA"
                     /locus_tag="ACL8LA_000548"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGS1812359.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(44954..46204)
                     /locus_tag="ACL8LA_000549"
     CDS             complement(44954..46204)
                     /locus_tag="ACL8LA_000549"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGS1812360.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(46271..47098)
                     /gene="purR"
                     /locus_tag="ACL8LA_000550"
     CDS             complement(46271..47098)
                     /gene="purR"
                     /locus_tag="ACL8LA_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGS1812361.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(47365..48306)
                     /locus_tag="ACL8LA_000551"
     CDS             complement(47365..48306)
                     /locus_tag="ACL8LA_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGS1812362.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(48296..49552)
                     /gene="rmuC"
                     /locus_tag="ACL8LA_000552"
     CDS             complement(48296..49552)
                     /gene="rmuC"
                     /locus_tag="ACL8LA_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGS1812363.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(49554..50216)
                     /locus_tag="ACL8LA_000553"
     CDS             complement(49554..50216)
                     /locus_tag="ACL8LA_000553"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGS1812364.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(50179..50835)
                     /gene="rpe"
                     /locus_tag="ACL8LA_000554"
     CDS             complement(50179..50835)
                     /gene="rpe"
                     /locus_tag="ACL8LA_000554"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGS1812365.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(50846..51724)
                     /gene="rsgA"
                     /locus_tag="ACL8LA_000555"
     CDS             complement(50846..51724)
                     /gene="rsgA"
                     /locus_tag="ACL8LA_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGS1812366.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(51726..52598)
                     /gene="rsmA"
                     /locus_tag="ACL8LA_000556"
     CDS             complement(51726..52598)
                     /gene="rsmA"
                     /locus_tag="ACL8LA_000556"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGS1812367.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(52849..53142)
                     /locus_tag="ACL8LA_000557"
     CDS             complement(52849..53142)
                     /locus_tag="ACL8LA_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812368.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            complement(53135..53776)
                     /locus_tag="ACL8LA_000558"
     CDS             complement(53135..53776)
                     /locus_tag="ACL8LA_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812369.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(53778..55808)
                     /locus_tag="ACL8LA_000559"
     CDS             complement(53778..55808)
                     /locus_tag="ACL8LA_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS1812370.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(55885..56181)
                     /locus_tag="ACL8LA_000560"
     CDS             complement(55885..56181)
                     /locus_tag="ACL8LA_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS1812371.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(56501..57400)
                     /locus_tag="ACL8LA_000561"
     CDS             complement(56501..57400)
                     /locus_tag="ACL8LA_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1812372.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(57472..58032)
                     /gene="rnmV"
                     /locus_tag="ACL8LA_000562"
     CDS             complement(57472..58032)
                     /gene="rnmV"
                     /locus_tag="ACL8LA_000562"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGS1812373.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(58032..58805)
                     /locus_tag="ACL8LA_000563"
     CDS             complement(58032..58805)
                     /locus_tag="ACL8LA_000563"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGS1812374.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(59110..60003)
                     /locus_tag="ACL8LA_000564"
     CDS             complement(59110..60003)
                     /locus_tag="ACL8LA_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HGS1812375.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(60287..60421)
                     /gene="rpmH"
                     /locus_tag="ACL8LA_000565"
     CDS             complement(60287..60421)
                     /gene="rpmH"
                     /locus_tag="ACL8LA_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGS1812376.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            60829..>60964
                     /locus_tag="ACL8LA_000566"
     CDS             60829..>60964
                     /locus_tag="ACL8LA_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1812377.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     H"
BASE COUNT        18426 a        12740 c        11096 g        18702 t
ORIGIN      
        1 gaccacaatt actcaatacc gaatacccct gaatggtttc ttttacatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatataagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcagaatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcctttt
      661 aaaatatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaatccgt
      841 tttttgatgg gattaaataa acatgacacg gattttaaga ataattcttt ctttgacacc
      901 gtatatgtgt aaagcttatt ttgcttgttt aaccaatata aagctttaaa agtcctcaaa
      961 cacctataaa ttgttgagtt tggaagtcca gtgacttgtg aaagcaaatc aacatctact
     1021 actttttgac cttttgtcaa taaaaaggca atccacgtta attgttcgct aggtgttaat
     1081 tccttaggga cttcagtatc attcgcattg agtactagtc ccaatggagg gaagaagagg
     1141 tttcccttaa agtctacaaa cggaactcta gcttgaagta attgcttttt ttcactgtct
     1201 gataacttcg aaaacaccaa aacaacatcc atattggctt tttcacccat agtgcgagcc
     1261 tgagtaacaa atgaactcaa actccccctt ctcttttctt taatcaataa aaatgattcc
     1321 ttatgaaaag tcatttgagt atactcaaac ctttttacaa aactgattgg taaagtcaaa
     1381 gtcagttcct caatactgac atcaacataa aaggattgaa aaattgacat tatctttttg
     1441 acattcatgt tttttcctta ttctatcatt tttattatat tttatcattc ggtataataa
     1501 aatataagtt ttttgataaa ataaccaaaa actactccct atcccctctc acattaccct
     1561 acatatcgtt tgacatgcga ctgatagttc aggaaaactt caaggagaac ttttctctca
     1621 tccactatgc aggacttact atttcacttc tactcctata ggctcaattt gacacttttc
     1681 tttttcgaat tgctcatatg cctcctgaat agtgagttcg atttatccaa atttattttt
     1741 gtctattttg atgaatactt ccacgaagat agtctgaaaa tgaatttgca ccaattttcc
     1801 ttctcttttt aagcatatta ttccagatat gattgccctc ttgttcagta ataaattacg
     1861 ctttaaacgc ttcaatcagt atatctcctg ttgtcatatg ttctaaagaa aattcttcta
     1921 catatgattt aacatctctt aggttattac ttcctaatat cccattatgc tttttcgcta
     1981 aggaaataga agccgcttct cccttaccaa taatcttgtt actatcatga tttcttgtta
     2041 aatctctata taatgcgtat tcttcagttc caatgtctat gctcacaatc tcagctgaac
     2101 ccttagctac caactgatct atcctagatt ttaaatgggg aattgtaggt atattgattt
     2161 catcatacac ctcttgtgga ataacaattt tacccgaata gagcttttct aaaagatgtt
     2221 cagtaccaac ccataaaaat actgaaatac aatctgcatc aataaataca cgactagtca
     2281 aacgacaact cccccctctt catctagccc atatacaata tcatatctga aagcatctag
     2341 taacagttcc tcatacttcc cttgcgaaat tctgttattt tctaaaagtt gttcagttga
     2401 attaacatat tatcctaata tcatttactt tttactttct gacaaaggac gatataaact
     2461 tgtatcatag cctaatcttg aagctgtctc tataacacta atatccatat ttttaatttc
     2521 ttctgcatca aggtatccat cattcctcaa tctatataac ataactttat gactgatacc
     2581 ataaaactga cccaatttta taatatcttc tacttcaaga tgagttctat tggcattttc
     2641 tctaatttcc tcaaccatcc tatacagtga agatgggaaa attaaaaaat aagaagcaaa
     2701 ctgatccgct tttctttcag tttcatctcc ttcaccaatc aagataagac tgactgaact
     2761 cttcttcacc tcatcataat aaagatgata cagttcatgt gctaaagaaa atctttacct
     2821 tcctaatgac atgtctgaat tgactgcaat gagactgaac tgagttcctt tataacagac
     2881 tccgctaata ttctttccga gtccataaaa taccagcgtc aaattttcta tcttttgtac
     2941 caatttaaaa atatctatcg gcgattcacc atcagctccc aacttttttc taagatttga
     3001 agctttatta cttaaatcca ttcgtgatat tcctttcacc tttttccatt ttggcaatca
     3061 tgctttgatt caaagacaaa tactcagcaa tcttatcttg agtaagattt gaagatattc
     3121 taagttcttt tattcttttt ccaacatcac atacatttat cacatacatt tatcatattc
     3181 atacacctct cataaaaaga atagcacact tttgtcataa ttttttaaat aaaaaaatta
     3241 tgacaaaaca aggaagcaat ttattgatgc tgcttaaaaa tctaaaattg atgagttcct
     3301 atcaaaaatc gtatcttcaa cctcaaaaca gtacttaaag ctatccgact cggtttacat
     3361 tgtcaaattt agattttatt tgagcataac tttctagttt gctttctgat ttttgtttaa
     3421 tatagtagca aacattgaag gaaatatctc cacaaaaaaa cgcatactat taagcttttt
     3481 caagacctaa taatatgcgc tgttctgatt tgaaagacat tccattatta ttttactgta
     3541 atcaagccat ctggctctac tgtgaattct ggcttgtctg ccagtgttcc gtctggtttg
     3601 aggtagtacc agcctgttcc gtccgctgac tggataaagg catttgatac catggcgcct
     3661 tctttagcgt ctaagtagta ccaagtgtcc ttgtacttga cccagcctgt cttcatggca
     3721 ccttcttcgt tgaaatagta ccacttatca gcgattttct tccagcctgt agccatttcg
     3781 cctgagttgt cgaaccagta ccagttgccg tctgtgtgct tcctccagcg gtctgcaagc
     3841 atatagcctg aactgtcaaa gtagtaccaa gtgccattga ttttctcaaa cttgtctttt
     3901 ggataagagc cgtctgaatg tacgtaccag tagccagtgt cattcttctg ccagcctgtt
     3961 tcaatcgtca agccgttctc aatatcatac ttaaactgct cacggctaat gccccatttt
     4021 gccaagtaag ggtatggatc cacatggtct gagtggttgt ttggttggtt attcgtgcaa
     4081 tactcgtgcg ttttaattcc agctaaactc cctgtatcaa gcgttttcgg caaacctgct
     4141 tcatctgcta gattgcgtaa gagttcgata taaaggcggt agtccgtcat gaactcttct
     4201 ttagttgaat ggctttcaat cagttcaacc gctgcatagg tttcagcatt ccaaccgccc
     4261 ccaacgtccc aggcaccatt atcaacaggt cctacctgca tgatgcaacc gttcccaaca
     4321 atgtgcgaga aaaaacctaa ttctgggtct ttccgccagt gataatccgc ttcattctgt
     4381 acggttgaat gcggattccc agttgagtgt gcgtgtactt gcctatatgg ttgcacgccg
     4441 acttgaggca aatctgttct taatttactc acattaattt ccatattcta ctccttatca
     4501 attaaaacaa ctcatttttt acaatccaga accagaaact cctttatttc taccttacaa
     4561 agaagacaat cttagtcacg attaggcttg tagatagaac ctcaaaacgc actattttga
     4621 cactgtaaat aggactgaca aggtctgcat tctatctaca ataacacccc agactaaaaa
     4681 gcttttcaaa agtatatttt tacagtctct atatgtcctt ttcataaata ctatacttta
     4741 ttatatcata taaaagaagt caaaagtctg ttaaactatt ttcaacacca aactaaagaa
     4801 gagaacacaa gttttttcga tgttcactag aggaaatgga ttttattcag taaatccaac
     4861 taggattgca ctttggttgc caaaattgcc tttccttctt ttatcaaggg atgacggaat
     4921 agtgagaagt acagttgaac tgtcatggca acccagataa gaggttcaca aaggataaca
     4981 cccttatatc ctgcccaagg aataatcaaa accacaaaaa cgatttttcc gattagttca
     5041 ataaagctag aaactagagg aaggatcttc tgccccaagc cctgcaagca attgcgataa
     5101 atcaacaaga ggctcaaaat gggataaaag gctgaactga tttgcagata gagacttcca
     5161 ttttctatca agtaaccatc tgtcgaacta gccaagaagg aaaccaaggc tggactggca
     5221 aaaaagagga aaatacaaac aaaaactgcc caggatatac ttaaacgact gccgattcga
     5281 agaccttgaa caatgcggtc aggtcgctta gctcctagat tctgagaagc aaaggtcgtc
     5341 attgatgcag aaatagcggt cataggaaga agggcaaagg tcataatgcg tcgagctgcc
     5401 gtctgggcac taataatcac tgcaccaaat gtattaacag aaaactgtaa aatcacactg
     5461 ccgatagata caattgaact catcaagccc atagccaaac cttgctccaa gagatccgcg
     5521 tacaagcttt tgtcccattt gaaatgttta aactgtggca agagttctgg cacactttta
     5581 cgaatataat aaaagcagag aaccgctgat aaaccttgcg aaataatggt agcaagtcct
     5641 gcggattgaa ctcccagatg caattgcgta ataaaataga gatccagaac cacattaacc
     5701 aaggcagaga aaatcagaaa tcccagggct gctagactgt caccaataga ccgcaacaag
     5761 cctgcaaaaa gattataagc aaagctgaca cctacacagg tcacaatcat agaaatatat
     5821 tgataagatt gaggaagaat ttctgcagga gtatctaagt attgcaagag aggatacaag
     5881 ccaagaaagc ccagcaacat aactagaatg ctcaaaagag cacctaaaat ccaggtggct
     5941 gctactgctt ccttgatttt agtgaaattc cgagccccat aataacgagc aatgacaatc
     6001 cccatgccat tgccaacacc aagtgtaaag cctacaatca ggtcaaaaat cgctgtcgtc
     6061 gctcctactg cagccaagga ttcttgacca agaaatcgtc caacaatcaa gacatcagca
     6121 gtgttataga gctgttgaaa aatatttgat agcaagattg gaaaggtgaa gcttaagagc
     6181 gagggaagaa tcggaccatg tatcaggtcc actgttcgtt ttttattcat aaagctatta
     6241 tatcagcttt ccagaggagc gacaagaaac agcaaactta tatatagcgg aatgaaataa
     6301 ggacagtcaa atcgattttt aacaatgttt tagaagtaga tgtgtactat tctagtttcg
     6361 atctattata gttggttgag tttggaatag tacgctgtag ctgctaaaac atttctagaa
     6421 attaatttga ctttcctaat aaagttgttc atatcttatt tcaagctact atatataatc
     6481 taaaaacttg aatcaaaaat cgaaaaagta gcggatttct ccactgcttt aacagcttat
     6541 tcttcaattt cgatttcaag ttcatcgcct aagtcaagcg gaacttcttc attcactgct
     6601 tcttctttct ttggctcatc ttttttgttt tcagtatctt gttctgaaac ttcttctcca
     6661 tcaatcaagc caaatttaga acggacttgc ttatcaattt catcaaagat ttctgggtgc
     6721 tctgccaagt atttcttagc attctcagaa ccttgcccaa ttttttcatc tttgtaagaa
     6781 taccaagccc ctgctttttt gataatatcc aaatcgcttg caatcttcaa aagctcacca
     6841 gtcttagaaa ttccttctcc gtacataatt tcaactacgg cttccttaaa cggtggagct
     6901 accttatttt ttacaacctt aatcttagtt tctttaccga cattggtttc tttttgatca
     6961 ccagttccct taatttgtgt attaccacga acatccaagc ggactgaagc atagaatttc
     7021 aaagcacgtc cgcccggtgt tgtttctgga tttccaaaca tcactccaac tttttcacgc
     7081 aattggttga taaaaatggc aattgttttg gttttattga tagaggcgcc aagtttacgc
     7141 atggcctggc tcatcatacg agcctgcaaa ccaacatggc tatctccgat atctccatca
     7201 atttccgcac gaggaacaag ggcagcaact gagtcgacta cgacaagatc aactgcacct
     7261 gagtcaatca attttcccgc aatctcaaga ccttgctctc ctgagtctgg ttgagacaag
     7321 agcaattcgt caatattgac accaagggcc gcagcataag ctggatcaag ggcatgttcc
     7381 gcatcgataa aggcagcaat cccaccttct ttttgcgctt gtgcaactgc atgaagggca
     7441 accgttgtct taccagatga ctctgggcca tagatttcga tgatacgtcc cttaggataa
     7501 ccacctgagc caagggcaat gtcaagagct aaagaacctg agctcatcac ttgcaccttt
     7561 tgctccgcac gttcacccaa acgcatgatt gatcctttac caaagtcttt ctcaatcaat
     7621 ttaagagcat cattcaaggc cttttcacgt tctgccccaa atttttttga aatttcttct
     7681 aatttttttg gttttttcgc cattctattc tcctacattc taatgatcct cagacctatc
     7741 tactattata tcaaaagtta gtcacttaat aaagccttgc gaactaggtt aaaggcatgc
     7801 ataaccgcaa tatgacgtac atctgctcgg cttctgcctc caatattaac cttgataacc
     7861 tcagttcctt gctcttgcgc caagcctatg aagactgtcc caactgggtg tccttctagg
     7921 ctatctggtc ctgccactcc ggtcaaacta attccaaaat cagactgggt cttgcttcgt
     7981 gcctgctcag ccatcttctg agctgtaaat tcagacacca caccatgttc ttccaaattc
     8041 ttggcaggaa tatccaacat ccttgatttt tcctccaagc tataggtcac aaaaccaccc
     8101 ttaaatatac ttgaagctcc agaaaaattc gccacggtag cttggaaaag acctgccgtt
     8161 aaactctctg cagccgcgat ggttttccct tgccttttca gttcttctac cacaatgctg
     8221 gctaaactag tttcttcccc ataaccatag caaaagtctc gtaaagaaat tccttcgaaa
     8281 gtctggcagt ccaagatttg attttccaag atatccagcg cttgattcgc ctcttcttga
     8341 ctgctagcct ttgttgacag acgtagagtg acttctcctg tcttggcata aggggccaag
     8401 gtaggatcga tctgattatc aattaaatca gccaaaatcg taaccaactg gctctcgcca
     8461 atcccaaaga aacgaagaac tcgggaatac agcttgctcc ctgtcatcaa cttgggtaga
     8521 agttggttta agaccatggg tttcaattca cttggcggac ctggaaggac gacataggtc
     8581 actccgtcta cttctaattt tcctccaaca gccagtcctg tttcgtttgg cagtggaatc
     8641 gctccttcta caatttgagc ttgtctttcg ttattcggtg ttcgggcata gtctggtcgc
     8701 agggcaaaaa agatatccaa cttctcctga gcctgaggat caaagactaa tgctttccct
     8761 aaaaatttag ctagggtttg tttggttagg tcgtcctcag ttggccccaa accgcctgtc
     8821 aaaatcacca gactgctacg ttgactggca atctcaagca aagacaagag acgaacttca
     8881 ttgtctccta cagccgtctg aaaatataca tctaccccaa tctcagctag tttttccgac
     8941 aaaaactggg cattggtgtt gacaatctgt cctgtcaaaa tctctgttcc aacagcaatg
     9001 atttctgctt tcatgtttcc tcctacctat ctattcgtat ttttttgaaa aaatcgcagg
     9061 aattttccta cgattgattt ttttatttgt atcaaaagtt aattatcttc atcaccaaca
     9121 ggtgctctgc caaataaatc ttcaaataaa accgcattgg tttcaagctg agtaacttct
     9181 tcttgtccca aagaacgtcg gagtatattt tgcatttcca acatatgtgc tctcgaaaca
     9241 atctggtaag aaacaccttg aagtatctct ccttcaccct gcaactgctg agtttcaatg
     9301 gttttaaatg aatctttata gcctagcaag ttagggatac tttttgcaga caaatcaata
     9361 ttggtctgca tattgtcact caaagctttt agaatctctt gataatgacc aacgctattt
     9421 aaactgagag ctttttccat gactttttga ataacttcac gttgacgttt ttgacgacca
     9481 taatccccct caggatcttg gtaacgcatt cgtgcataga ctagggcttc ttctccccca
     9541 atatgttgct ccccaacacc gatagaaata gtattaaatt cttcttggtc actgatagaa
     9601 attgggaaac ctaggatatt attgactgta atacctccta ctgcatccac tagtttttgc
     9661 aaccctctca tattgaccat cacatagcga tcaatatgga tattcatcat tttttgaatg
     9721 gtttctatag caagctctgc tccaccatct gcatatgctg agttcagttt cgcttcatga
     9781 gcctgaccat tccctgattc aatgcgcgtc agaatatccc gctctaaact catcattgtt
     9841 gtttttttcg ttttaggatt cactgtcatc aagatcatgc tatcacttct accgacccaa
     9901 gtttcagttc gttcaacatt tccggtgtcc actcccatta acagaatggt tagaggttca
     9961 gtcgcttcaa taaccttggt ttcttcaccg atttttttat aggttttagc taaggtttct
    10021 gtcccttgtt gataaatagt ataagcaaaa acacctactc ctactacagt tacagaaagt
    10081 aaagctagca ccattccaat aattttttta accatatttc tactaaccta tcagtttacc
    10141 catcaagtaa acatcgataa atttcccttc ttctatatat gccccacgct cttggctacc
    10201 ttcaatgaca aagccatgct tttgataaag atggactgct gcttgattac gagtttggac
    10261 agtcagttgg agacgacgca gaatgccact tgcttgtgcc cactctatcg cttcttctag
    10321 caacaaactt cccaagccat tattccaata tctttttcca atcacaatga agagttctcc
    10381 aatatgacgg actctcttac gctggtcagc tgtaatattt acaataccag caattttgcc
    10441 atttaagaat gcaagtaagg ttatctgatt gtccgaacta gcttgcttgt tgaggaatat
    10501 ttccatctcc tcactagtca agagaatacc atctccgtct aggctggtaa agtctgtctc
    10561 caaactcaca cgatttaaaa aggccactaa ttcagctgca tctttgggct ctgcttccct
    10621 aatgagcaat tcatactcca tattgaagct cctctaacaa tttctcagca cgcaaaccct
    10681 ttgcctgaaa atttaaacgg cgtccatctg cttcttttag aatttccaat tctaaataag
    10741 catctggcaa ggcatctcct aagagattgc cccactcaat aacagtcacg ccgccaccaa
    10801 agataaactc atccaagtcg atagaatcag catctccttc aatacgataa acatctaggt
    10861 gataaagtgg aagtcgacct tcatactctc tcacgatagt ataggtggga cttttaatca
    10921 tttgagaaat ctgtaatcct tttgcaagtc ctttagtaaa ggtcgtttta cctgcaccca
    10981 gttctccagt taagattaaa acatcattct ttgctaatag atggcccaaa cgctccccta
    11041 aggcttgcaa ctcttcttca ttttttgtgt acatactctt attataccaa aaacttttct
    11101 tttgtgtcta ttttcctact aaacttatca tcataacatc cataaaaaac aggctttctc
    11161 taaaagaaaa tgagcgtaac aatgaccaat acaagatctc ggaaaatatg accataaaag
    11221 gaaacttcct tcttaaccga atttgggaca agataggctg caaaaaacaa gcccagtcca
    11281 atataaatca gaagtgagac aatggtcatt ggatttctta agaaaagaag tgttgctaaa
    11341 atagtcacca acactgtctt ttttctgtct agcatagcaa gaaaatcgcg cacgtatttt
    11401 ttcaagggta aaaaaatcag caaatctagc ccaaatagga aaaagaagga tggcaataaa
    11461 aagtcaacta attcttgctg cagcgtattt ttgatgaaca agttatctga caaaacaaga
    11521 acagctccta acaaattaat taagagtaac atactgtaaa aaagcttcac agacttctta
    11581 ctggctagga cactatggac ttcttgctta cgggtataaa gataatttac tccagcacag
    11641 attcctgaaa cgaaaaccat gcttccgatg aaaaaaactg tactttgttt aaaggacaag
    11701 atgcattcct tccataggaa acagctactc aaactgattt gaattaaagc taacaaaaat
    11761 aagattctca ttgatttcat cttctctctc ccttcctacc aatcattata ctaggagaaa
    11821 agagagaact gtttctaatc ttctcaaatg tctctttaag acgctaaaca aacactagag
    11881 actaatactc aatgaaaatc aaagatcaaa ctaggtagct agccacaggt tgctcaaaac
    11941 actgttttga gattgcagat agagctgacg tgatttgaag agattttcga agaatataaa
    12001 tttgaaatca tgaaaatccg tcaaacgggt ggttgttttg tctcgcacct cacggagcga
    12061 gacggactca gagtcacata attataaggc tgatagtatt aatctaacta tcagccttac
    12121 aggttattta acgtttcaga aaaactataa tgtcaagatt aactaaacag tatctaattc
    12181 cttcaaataa ttttctatct tcatcaacat taaaggattg ttataaatct tacataactc
    12241 tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg ctccagcatt
    12301 tcctacaaga ataagtagag gagccaattg gtagcttgtc tgtctttgtt tacagagttc
    12361 aatcgtttca agagcttctt ggatggcttc attatatttt tcctttgata ctaggtagtg
    12421 agcgtagttg taacgaactc tgatgtagcc aaataaaaac tcttgatggt ccaaattttt
    12481 tgtctgatac aactctatta aatgagagta gtttgcctca tattcttgtt cacgacccac
    12541 taaggaatag aaattagata gagtattcaa cgcctttaaa taaatcagag tatttgaaga
    12601 gacttttaat aatatatttt ccaatgacga aattgcctca cacttactgt catattgata
    12661 gaagtcaatt atagatttaa tccattcaag gtaagttcgg tcttctaatg ttagaaaagt
    12721 gcttcgttct acctctattt tataaagata ttctaaatcg tcataatttc tgtcatctaa
    12781 taggcgagca gatagatgtt tgaaattaga gaggttagac ttaacttcga tttgttcatt
    12841 gaaaaagtaa tccaaaggga cttcaagtcg ttgagagagt ttgaataaca agtctgcgga
    12901 gggaataaaa tgacctcttt caattttact aatctggctt tgttcacaaa ttccttctgc
    12961 aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct ctgcgagtgt
    13021 attcattaaa gataataaac cttcctattt gcttaatttc attataaagt ttttagttca
    13081 aaatagcaag ttaaaggaat aaataattct ttatggtaat aaacaaaaaa aaaagttaaa
    13141 aattacataa aaatacttga ctaaataaaa tatatctgtt agaataaaaa caaggaaaaa
    13201 gaaaggggtt tcattgcatg agaaaaaaac gtggaattaa aaaattagtt acatttgcat
    13261 tgctaggtgt ttttatgttt agtaatacaa ttccttacca acagtttatt cagaagaata
    13321 aacaattgga gattcgagtg taatcgcaaa agaagtccaa tggtcttgat gttgggaagg
    13381 ctgattaaaa cccgatattt tgcacgagaa tgagagaatt ataatttatc tgaaaaaacg
    13441 aaaaatatag agcaacttat ttaacttttt ataaggagga gttattactt atgaccaact
    13501 ttaattcaaa cgaaaaattt tgtggaaaaa gtttaaaatc attgtctgct gatgaaatga
    13561 gtttaattta tggtgcatct gatggtgcag agccacgttg gacgcctact cctattattt
    13621 tgaaatcagc tgcagctagc tcgaaagtat gcattagtgc agctgtttct ggtattgggg
    13681 ggctagttag ctacaataat gattgtttgg ggtaaattaa aatgaaaaat gattttgtaa
    13741 tcggaaagag tttgaaagaa ttatctcttg aagaaatgca attggtttat ggtggcacag
    13801 atggtgctga ccctagatca acaattatat gttctgctac attaagtttt attgcatcat
    13861 atttggggtc agctcagact cgttgtggca aagacaacaa gaaaaaataa aatattcaat
    13921 tttaaaaata aaagttgctc tttattttcc tctctcgtaa ctaataattt tgagagaggt
    13981 ttttttttaa aacaatagta agtcgtatag gtttgaggta agtaaatgaa atataaaatt
    14041 aaaaatgtgc ataaaacatt gtttattaag gaaagatatc aaatattatc tgaacgaaat
    14101 gataattcct taaataactt ttcagaaata attggcgttc tacaatgtca attatttctg
    14161 aaaaaacttt tgaagaaatg ttgaaattaa ataattacaa taaaaaaata ttttctaaca
    14221 ctgtgaacta tgattatacc gaggaacaga taagactata ttcagaatat gccaaaaatt
    14281 cggaatggta taaatttgcg gagggttcat ttgaaatatt tagaaaacat ccccaaagaa
    14341 ttaattttaa gaaagatttt tctagaattt tggaaccctt tattattaat ttgcttaaat
    14401 taatcaataa ttatctagag aataaagaat acgagtggat tgacaagaat ggaaatattt
    14461 tttcctctct agtattttat ttagaagatt taatctatcc ttggattgtt aaacctttgg
    14521 ttttagagat aaattcattg cgtgaaaaag gtttacttga aggggaatcg gagcagcaac
    14581 ggtacaaata ttttataaca ttgtttgaca aggaagagaa tatattaaat ttttataaca
    14641 aatatcccgt tttactgagg caaatatcgg agtcttgtct tcggttctat acttatttta
    14701 tagaaatttt atcaaattta gaaaatgatt ttagtgtgct agaagaagaa ttagggctaa
    14761 gggggaaatt aaatgatata aaatttggaa agggtgatac acacagccaa ggaaaaactg
    14821 ttttgatact cttctttgat gacgcgaaaa ttgtttacaa gcctaaaaat ttaataatca
    14881 ataactcact aaatactatt gctgagtata tccgaaaggt tgatgaaaaa attaggataa
    14941 gaatacctcg aactattgct tattcggatc acagctatga agaatttatt gattatctac
    15001 ctctagagca aaagaaaaaa ttacctgaat attattataa ttttggtgtg cttttagcat
    15061 ttatatattt atttaatggg agtgatatac attttgaaaa tttaatttcc tatggagata
    15121 tgcctgtaat aatagacttt gaaacaatgt tacagcaacc tctatttgat gataaaactg
    15181 gtcagagttt gctggatacc ctgtttcaca gggtgactcg aactttgctt cttccaacag
    15241 agggagtaaa aagggaggat ggtcttgatg ttgagatgag tgctcttaca ggaaatttta
    15301 agaaggatgc ttttaatggt caagttttaa ttaatttaaa tactgataag gtaaagtttg
    15361 atattgggaa aattgatttt gaaggtggga aaaacctgcc tgttcgggat ggtgatatag
    15421 aatttgataa gtatataaaa gatttcaaaa aaggttttag ggatttttat ctgatatttg
    15481 aagagttaaa taaaacagaa gaatttaaaa tgctattaaa agcaaacttg tatggtttaa
    15541 agacaagggt actttttaga gacacaaata gctatgcttc agtactatct tttttatatc
    15601 atcctgattt ttatgaagaa atgttagata gagaaaaagc attggaaaat ctctggagca
    15661 ataagttttc taatcaaggt attgtagcaa gcgaatgtga acaaatgagg ttattagata
    15721 ttccgatttt ttatacagat acaaatatca atgaaatcta cgatgatttt ggaaatcatt
    15781 ttatttccct acatcaattt tctggatatg actattttat gaaatattta cctaataata
    15841 atttggaaaa tttagaaaag caacttgata tcattgaaat ttattttcca agctttgatc
    15901 agcgaaaaaa aggatataaa ttaaacttat ctaatagaaa aaaactccca aatgaaagtt
    15961 tagttactat tctgcataat gaagtagaaa atattgcaga ctctttggtt attaataata
    16021 tttatgacga atatccaatt tggagtatac ctgttaattt taaaggttct aaatggtgtc
    16081 ttgaacaaac gggtaatgat atgtataggg gtagagctgg aatattactg tttatgtact
    16141 atgtaaaatt attgaaagat aaatcatcat atactcaact gtacaataaa atgttatctg
    16201 caattccgcc atctgtttct ttatctaaga ccacatttgg tttaactggg gatataggat
    16261 atttcattgt tctatccatt atagaggatt acgatacaaa tactctcgca tgcttaaatt
    16321 atataaagag tgttttaact gagcttgaaa agactgattt tgcaagtgtt tcaaacaagt
    16381 ctgactatat taatggtctt ctacctttaa ttaatacact ggtaagatat tataggagag
    16441 gaatagagaa ggaacgtgtt ctaaggctag tattaagttt aggtgatacg ctatataatg
    16501 atgtaagcga gagagataat agtaattttg gatatagttt tggccatgga ctatcaggtg
    16561 taatttttac attaaaaaat atttataaag tgacaagact taaaaaatat aaagaattaa
    16621 ttttacaact agttcctagg gagaaaatca aaattcattc tttgaagtgg tgtagtggag
    16681 aatttgggac attagttaat catccccaaa gtgttccaga gggaattgta gctagtttag
    16741 ataatgatac attttgccat ggaagtatgg ccataataaa tttttacatc gactacatga
    16801 aagaaatgga tactagtatg gaattggagt ctttattgtg tagtgttgta atgaataaat
    16861 caaaagaagg agagtatcgt atcgtccaaa caagcaattt cccagatttt tctttgtata
    16921 caggtgtgac tggtattgct tatacttttt taaggtatat cgcactgaat cagaatgaca
    16981 aaaagatagt tatcccctca ttacttgaaa tctagcttaa tttatagttt tgtttctaaa
    17041 atagaggtgt ccactcatta agcaatataa aaataccaag attgactagt ttaatatgta
    17101 attctataaa atatttttaa tagtgtagct tttcgttgag ataataatat aataaagaaa
    17161 agttctattc taattgctaa acggcttgag gtatatgcta ctgttataga aatgatttta
    17221 aactaaaatc aaaattactc caaaaatagt attgattttg gtttaataca aaacatatgt
    17281 aataatgtga gtcaacaaaa gtctgttttt gtaccatact atcttgaaaa atctactaaa
    17341 ttcaaattga gaggatgaat taaaatgaat aaaatattta tatatgcagg agtgcgaaat
    17401 cataactcta agactttaga gtatactaag agactttcaa gtatcatttc tagtagaaat
    17461 aatgttgata tatctttcag gacacctttc aattcagagc tagaaatatc taattctgat
    17521 tcagaagagt tgtttaagaa aggaattgat aggcaaagta atgctgatga tggaggtgtt
    17581 ataaaaaaag agttattaga gtctgatatt ataattatct cttctcctgt atatttacaa
    17641 aacgtatctg tggacacaaa aaactttatt gaaaggattg gggggtggag ccatcttttt
    17701 cgattagctg gaaagtttgt tgtaacatta gatgttgctg agagtaacgg ctctgataat
    17761 gtgtcagaat atcttagaga tatattttca tatatgggag gacagatatt acaccaagtt
    17821 tcaattacca attcactcaa agatattgca gaggctcagt tgatggaggc aacttataaa
    17881 attgaagatg tacttgaagg gaaaattaag tacaaaacaa cggattatca agaaagagct
    17941 taccagacat taaaattaat tttagaaaat tatgattccg aacattttga gaagatgtat
    18001 tgggagaaaa aacgcctttt tgaagctaat agtttagagg aatggtatta tgttgaaaat
    18061 attaaataac tctgtcatat atttatgttt gattccgtta ttgttcctat tattgataat
    18121 ttttccaaat gattcattaa tctattattt tcgattgatt ttaatttcgt taatatcaat
    18181 tacattacat gaactaggtc attttttagt gggaagatgt ttatcttata aactagagat
    18241 gcttgctact ccctttttct tctatttcag aaaaaaaata tatttcaaat ttccagtgtt
    18301 attagcattc ggttattgtc aaatgagtaa tagaaacatt actaatgaaa aaaatagtga
    18361 taggaatttg gtgttctact tctttggggg aggtggagct aacctaattg tcgcaatatt
    18421 agcacttttg ggatttgtgc cgtacgcatc agaatttttt attttgaata taattctttt
    18481 tctagttacg gtatgtttac caattgatgg aacagatggc aatgctatta gagagatagt
    18541 tttgtattca aaagattcaa aaacatatca gagattcttt gcgaacagtc tctataataa
    18601 tccttatata acaatcgacg attttgcaaa attaacctct aaagaaaaat cttttttttc
    18661 aaaatttgaa aaatgtttga taaaccttta ctttgaagtg aaaggtgaag ggtcagcatt
    18721 tacaattgct aatcatgaag tttttgattc aaatattcag gaaaatatta tccatgagta
    18781 ctataaatta ctacataaat cttctgattg gattattcca cagaacttat ctttagaaga
    18841 agttgttttt accttgtcaa actatattta ctcgaaaaat aataaatatt tagaaaagat
    18901 taagtatcta aagaaattag tagattttag acaagaagaa attatcgact ttattttgaa
    18961 caaggaggaa gtattgtgaa aattccaatg atatatcaaa tggaaaattc agaatgtgga
    19021 ttagcatgct gtgccatgat attgaactat tttaaatatg agatttcttt aaatgaacta
    19081 cgtgaaatct acccatcatc cagatctgga tattctctcc tatctataag taaagtttta
    19141 ggagatttta atataagttc tcatgctttt aaagcttcgg taagagattt aaaaccgctc
    19201 agtttcccac tcatttgctt ctgggagagt tctcatttta ttattcttga aaaaattagt
    19261 aaaaacaagt tttatatttt agatcctgca aaaggcaggc agagaatgtc aataagtgaa
    19321 tttgaaaggc attattcaaa tatcatttta acatttaaaa agttagatag ctttatgtct
    19381 cgtaaagata ataagaagtc gcctgtttta aagtattttt ttaagtatag gaataagcta
    19441 gggattttat tttttgtaac agcattattg tatgtaatac aatcattagt acctatagct
    19501 aatagataca taattgacac gaatttcaag gacgattcgt attcgtctag aatgttattt
    19561 actatattat ttatatttac tgtttcattc tcactaatgt atttattaag acagatatat
    19621 gttgcatcct taaaatatat aatggataaa gagattagct atgattttat gaaacatttg
    19681 atatatttac cttacagttt ttatgaaaaa cgtactttag gggatatact ttttagagct
    19741 aactctattg tttatataag agaaatacta tcaaataatt ttatagcagc tatacttgat
    19801 ttgttaatga ttgtggttta tgctgtggtt ttatttagct tttctaagta catggtaatc
    19861 tttttaatat cactaagtct agctctatct attgtaatgt atccaatcat aaaaatctca
    19921 aaaaatttaa ttgataaaaa tataaaagaa aaggttaatg ttcaaaatat tacttccgaa
    19981 gtaatttcta aaaatagtga tattaagcta actggagaag aggaattttg gattaacaaa
    20041 tgggataatt ttaatacaaa acagctcatc ataggtcgaa aacttgatat acatttatca
    20101 attgttagta gtataacgaa tgttttacaa attattctcc ctgttttgac ccttattgta
    20161 ggtgtaaata taaaaacatt cgaacaattg acgttaggac aaattgtagc aataagtaca
    20221 gtctcaccat actttatttc tcctataatt tctttaagtg ataactatat acaattaatg
    20281 ttattaaagg gatatttttt aagaatagag gatgtgttta atactaaatc cgaattaatt
    20341 ccagaaagag tcagtcaaga tataaaattt gataaaaaaa tagaattaaa agatatttgg
    20401 tataaatatg gattatttga tgattatgtt ttgaaaggaa taaatgttac tattaaaaaa
    20461 ggagaaactg ttgctattgt tggagaatca ggttcaggta agagtacatt agctaaaatt
    20521 ttattaggtt tattagaacc taatattggt tcaatagaag ttgatggagt agaaaaagaa
    20581 gaaattggtc aaacattgta tagaaagatt tttggagcag tgttacaaaa ttcaacccta
    20641 agttatggta ccttaagaga gaatttgaca tttggacact ttgtttcaga tgaagaatta
    20701 atgacaaatc taaattcaat tggtcttagc aatgtagtta aatctttacc tcttggatta
    20761 gagacaatca tcgctgaaga aggtaataac ttttctggag ggcagcagca aatgatactt
    20821 ttagctcgtt gtcttttgtc gaaaccttcg gtagttgttt tggacgaagc aacaagtagt
    20881 ttagataatt tatctcaaca aattacaact tcttacttaa gtgaaatcgg taccactaag
    20941 attttaattg cccatcgact agatactatc aagtctgcag ataagatctt agtaatgcat
    21001 aatggtgaaa ttgtagagat tgggacccat agagaacttc ttgaactagg aggcatttat
    21061 aagcaattgt attcaaataa ttagtttttg attaaaaggg taaatttatg aagattatga
    21121 aaaaaaaata ttggacttta gcgatattat tcttttgttt gttcaataat tctgttactg
    21181 ctcaagaaat acctaaaaat cttgatggca atataactca cactcagact agcgaaagtt
    21241 tttctgaatc tgatgaaaaa caggttgact attctaataa aaatcaagaa gaagtagacc
    21301 aaaataaatt tcgtattcaa atcgataaga cagaattatt tgtaacaaca gataaacatt
    21361 tagaaaaaaa ctgttgtaaa ttggaacttg aaccacaaat aaataacgat attgttaact
    21421 ctgaaagtaa taatttacta ggcgaagata atttagataa taaaattaag gaaaatgttt
    21481 ctcatctaga taatagagga ggaaatatag agcatgacaa agataactta gaatcgtcga
    21541 ttgtaagaaa atatgaatgg gatatagata aagttactgg tggaggcgaa agttataaat
    21601 tatattctaa aagtaattct aaagtttcaa ttgctatttt agattcagga gtcgatttac
    21661 aaaatactgg attactgaaa aatctttcaa atcactcaaa aaactatgtc cccaataaag
    21721 gatatttagg aaaagaggag ggagaggaag gaataatatc agatattcaa gatagattag
    21781 gtcatggtac ggctgttgta gctcaaattg taggggatga caatattaat ggagtaaatc
    21841 ctcacgttaa tattaacgtc tatagaatat ttggtaagtc gtcagctagt ccagattgga
    21901 ttgtaaaagc aatttttgat gctgtagatg atggcaatga tattatcaat cttagtactg
    21961 gacaatattt aatgattgat ggagaatatg aggacggaac aaatgatttt gaaacatttt
    22021 tgaagtataa aaaggctatt gattacgcga atcaaaaagg agtaattata gtagctgcat
    22081 tagggaatga ctccctaaat gtatcaaatc agtcagattt attgaaactt attagttcac
    22141 gcaaaaaagt aagaaaacca ggattagtag ttgatgttcc aagttatttc tcatctacaa
    22201 tttcggtcgg aggcatagat cgcttaggta atttatcaga ttttagcaat aaaggggatt
    22261 ctgatgcaat atatgcgcct gcaggctcaa cattatctct ttcagaatta ggacttaata
    22321 actttattaa tgcagaaaaa tataaagaag attggatttt ttcggcaaca ctaggaggat
    22381 atacgtatct ttatggaaac tcatttgctg ctcctaaagt ttctggtgcg attgcaatga
    22441 ttattgataa atacaaatta aaagatcagc cctataatta tatgtttgta aaaaaaattc
    22501 tggaagaaac attaccagta aaaaatggta taaaagtgtt aaatatacca aacgtattga
    22561 gatatgattt gaatatgtta caattagaat ataaaaatga acaaagttgg gatagtttca
    22621 tagataatgt taatttaatt gagttggaag agagaattca aactactatt ggaattaaac
    22681 aaataaacac acacaatatt attactattg cccgagaagg gtactctcaa aattatttac
    22741 ctaacacttc agaaaataca tataattcat tacaagtcag tttagttgga gtattactac
    22801 tttttataag tatggtaaat attttatggg ctaaaaaaag taaatgaaaa taaaatttgg
    22861 agccctctga aaaagtaagt cctacagttc aactaaaatg agtcaaaaga tgaatcacct
    22921 tgatgtaggg gagtttgtct tattgctgcc tgaacacctc cgttcagagg aagaacatta
    22981 taaatctgtt tttgaagacg acttaaccag tcgcatatct agtcaagatg aacgacagca
    23041 aatgactgct acggtaggtt atttagaatc aggtcaggat cgttttgtgt ataatacgac
    23101 ccctatttct taccagcagt ttttgaaaga tccaatcatc attgttataa caccccaatc
    23161 aactggtcca cagtccattt tgttttggat agacgcagta cagaactacg ttctctttaa
    23221 tcaattgtct gatgcccagg agcttatcca gagacaaggc attgaaaatt gggtctcaga
    23281 aatgcaaaca ggttaccaca actacatcac attattggat aatatccaga gggaacgttg
    23341 ggtaatgcta gcaggagctg tgcttgggat tgcaacttca atcttgttgt ttaacactat
    23401 gaataggctc tactttgaag aatttagacg tgccattttt atcaaacgca ttgcaggtct
    23461 caggttctta gaaatccatc gcacttatct ctttgctcaa ctgggtgtgt ttttactggg
    23521 atttgttgcg agtgtatttc ttcaggtaga gataggagtt gctttcttag tcttgttact
    23581 ctttactggt ctatctcttt tacagttaca tgtccaaatg cagaaagaaa acaagatgtc
    23641 catgcttgtt ttgaagggag gttaatatga ttgaacttaa acaggtgagt aaatcttttg
    23701 gagaacgaga gttattttcg aatctttcaa tgacatttga ggctggaaaa gtctatgcct
    23761 taattggttc aagtggtagc ggaaaaacaa ccttgatgaa catgattggg aaattagaac
    23821 cttatgatgg gacgattttt taccgaggta aagacttggc caattataaa tcaagtgatt
    23881 ttttccgtca cgaattgggc tacctcttcc agaactttgg cttaattgaa aaccaaagta
    23941 ttgaagaaaa ccttaagcta ggtctcattg gtcaaaagtt gagtcggtcg gaacagcggt
    24001 tgaggcagaa gcaggcttta gaacaggtcg gcctggttta tcttgaccta gataagcgca
    24061 tctttgagtt atcgggcgga gaatcgcaac gggttgcctt ggcaaaaatt atcttaaaga
    24121 atccaccctt tattctggca gatgagccaa cagcttcaat agacccagca acctctcagt
    24181 tgattatgga gattttgcta tctcttcgag atgataatag gctaatcatt atcgcaacac
    24241 ataatccggc aatttgggag atggctgatg aagtgttcac gatggatcat ctgaaataaa
    24301 aatccttgtt tttaattgca cgatgagtta ctgaaatatt atcatgaatc aagaattgga
    24361 gttaatttag aattgtactt tattaatatt gaggtaactt tttcttgata aaggaagaaa
    24421 taatggagag gaagttagaa tgaaaaaatt cgacaattat attattgaga agccttgcga
    24481 ttctaattca gataaactgc aaaaaatctt aataattgaa agtttggtag atgatatttt
    24541 gcaattttct ctcagaatca ataatagtgt aggagagatt ttcctcctac aaccgtttta
    24601 aaagaaaact atctttattc catgttattt tgaggaagat attgtgaaag tcaaagatga
    24661 tgataaagtt gagtggaatt tgttagaatt tcaaaaattt agagcatttt tggcttagta
    24721 atctgtgttg aaggctcaaa acctatggta aaaaagtagc tttgaaaacg tattgcctcc
    24781 aaagatttag ttaaataatg atttaacaca aaaagaaatt attgaagttc tggaaagatg
    24841 ttgtttcagt attgagaaaa ggtgggaaaa acttgcgatt ttcacagaga aaggaagaaa
    24901 aagtatagaa atatagtcaa ttgaaacaag aacaggataa aagaaccttt tgtgccatat
    24961 ttttctcctt tcgctttaca attggattga acacctttat tgtatcgcgt ttggagtttt
    25021 tttggtataa ccttcgacgc acacccgcat agcgggtgtt ttttttgtct cgcacctaac
    25081 ggagcgagac aaactaatag tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag
    25141 tttgatatca tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt
    25201 taaccaattg attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga
    25261 ctcttctgaa tctgaaccat gtacaacatt ttggataatc tcattttctc cagcagcttt
    25321 tgcaaaatca cctcgaatag tgcctggtaa agcttcttct ggacgagttg cacccatcat
    25381 ggtccgccaa gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga
    25441 agtcatgaat tcacgaatcg gtgggtaaaa actctgacca accaagtcct gatagtgctg
    25501 gtcaatcaac tcttctgaaa cctgtgaacg aaactccaat ttttcgattg taaatccacg
    25561 ttgttcgatg cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat
    25621 gataaagaat gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca
    25681 catttcgtgg aaaaatggag aaagttttca gaagagagaa tgagagaacc ctcgggttct
    25741 ctcattctct cttattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact
    25801 tctgttgttt cttcatttcc ttcttcctct actggaggat taaggtattc ttcttcgttg
    25861 acagcatgtg gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta
    25921 ccgatgataa cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg
    25981 tcagtaagga cacgagttgt ttcctggaag gaagccgctg acaagaaact gtttgtttca
    26041 agtgaggctt tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg
    26101 ataaggacat ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga
    26161 agatctgtat cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc
    26221 tcgatgtgtt tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg
    26281 agaaggtacg tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata
    26341 gaaccttctg tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga
    26401 gctgtaaatg gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg
    26461 gtacgagttg atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtaataacc
    26521 gcttcccctt taggattgcg ggcttcaaag atttcttgga cacgaggaag accctgagtg
    26581 atatcggtat ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca
    26641 ggttccccga tagattgggc agcgattgta ccaactgctt caccaacttc aaccgcatca
    26701 ccagtcgcca agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta
    26761 aatacagaac ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct
    26821 tctgtaatca attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt
    26881 ttcttagtgt aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct
    26941 gcgatagaac ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg
    27001 tcttgggcaa cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc
    27061 gtatcggtca tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa
    27121 ccttcgcgga agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc
    27181 agaccacgca taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc
    27241 atcatcataa cgattgggtt cttaggatct tgattagcaa tcaagcgttt ctcaagtttt
    27301 tcacgggcag cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata
    27361 ccacgacgga attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca
    27421 gccttgtcat caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag
    27481 tggtggtaac cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa
    27541 cgtttgaaga tttcagcgat gatatttccg aggtttttct tcttgaatgg agggttgagc
    27601 tcaagattgc tgatagcttc cttgatagct ccaccaagtg gcaagaagta tttagctgga
    27661 acaccttctg tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg
    27721 atatcgttga agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct
    27781 gtccaaggct tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa
    27841 ccattgcggt aagccataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg
    27901 cgaccagctt cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta
    27961 actaccggtt tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga
    28021 gcttctgctt gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag
    28081 tcagcattgt aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaatcaag
    28141 actggctcga aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact
    28201 gggtgttctt taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc
    28261 aagcgtttag ctgctttgac gttttgcacg atatcacggg caacgatttc acgcatgaca
    28321 aatggtttaa agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga
    28381 gttggaccaa cggcgataac ggaacgtcct gagaagtcaa cacgtttacc gagcaagttt
    28441 tgacggaagc gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta
    28501 cctggtcctg tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct
    28561 tgaagcatac gcttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga
    28621 gccaaacggt tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca
    28681 aaacggccac catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg
    28741 ttaagaatca tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa
    28801 cgacggatgg ctttgacacg tttttgtcca gtagcagttt tcaattcttc tttgagttca
    28861 gcaatttctt tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc
    28921 ttagcaacaa atgaaccata accatattcg cgcaagcgct ctcggtattc gcgctctgtc
    28981 atgatagact tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag
    29041 tagataactt cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga
    29101 atccccttga agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc
    29161 tcacgacgaa ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg
    29221 taacgaatgc gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc
    29281 acttcatcaa agagtccttc acgttctggt ttcaaggtac gataattgat tgtttcaggt
    29341 tttttgactt ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc
    29401 atacttttaa aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg
    29461 cttattcttg ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt
    29521 cagcttcaaa ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat
    29581 ctacgtggat gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt
    29641 catcttcgtc taggacacgc atgtcaagac caagagattg caattctttg acaagaactc
    29701 ggaaggattc tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg
    29761 ctttcaaacg tccgttgata tcgtccgact tgtaagtcaa gatttcttga aggacatttg
    29821 acgcaccgta ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact
    29881 gagccttacc tccgagtggt tgttgggtaa cagttgagta tggtccgact gaacgcgcgt
    29941 gcaatttatc atcaaccatg tggtggagtt tgatcatgta catgactccg acagaaacac
    30001 ggttatcaaa cggttcacca gtacgtccat cgtaaaggat cgttttggca tcgctatcca
    30061 tacctgcttc tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg
    30121 tagcgatgtg aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac
    30181 cgatattcat acgtgatggt accccaagtg ggttcaacat gatgtcgact ggagttccgt
    30241 ctggaaggta aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt
    30301 gacgtccggc cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa
    30361 ccaacatgtt aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat
    30421 cacgaacgac accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag
    30481 acttgtctcc aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct
    30541 taggtgttac tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa
    30601 tccccatttc gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga
    30661 tttcttcagg cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag
    30721 atgtgtagac atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt
    30781 aaccttccca agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt
    30841 ccatagaagg tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacga
    30901 gagtgcgttg gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa
    30961 catccaatga accatcttca cgacgaactt ctaccttgtc agcatctgcg taagtaactt
    31021 taccatcata ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac
    31081 cagtaccaac gtaaggtgcc tgaggattaa tcagtggcac agcctgacgt tgcatattgg
    31141 ctcccatgag ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa
    31201 cggcaactac ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt
    31261 ggttgacccc ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttcattca
    31321 gacgagagtt agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa
    31381 tttcgttcgt gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac
    31441 catatttgtt caagtgtccg taagatgaca agttattgat caaaccgatg ttaggtcctt
    31501 caggtgtctc gattggacac atacgaccat agtgagtgta gtgcacgtca cgcacttcat
    31561 atccagcacg gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag
    31621 acaactcaga aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga
    31681 attctttaac tgcagctgtt acaggacgaa tattgataat ttgttgtggt gtcaagactt
    31741 cattgtcctg aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac
    31801 gtacttggtt ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga
    31861 tatcatctac acggccaagt ccttcagcca agttgaggaa gtagctcatc tcagcaagga
    31921 tatctgcagg agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta
    31981 cgacgcgatc tggatcagtt ggagcaataa ccttgaattt ttgaagaaca acaggctcag
    32041 tcacaacggc tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc
    32101 tttcaatgct ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc
    32161 cagtttcagg gtctaccaat ggctctgcaa tggtttggtt gagtaaacgt gttttaacat
    32221 tgagtttttt attgattttg taacgaccaa ctgctgccaa gtcataacga cgtgggtcaa
    32281 agaagcgagc tacaagcaag ctacgtgagc tttcagccgt cttaggctca cctggacgaa
    32341 ggcgttcgta aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga
    32401 tatctttttc aacagtgttg cgaaccaatt cgctgtcacc aaagatatca aagatttcat
    32461 catcacctga gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt
    32521 cgatacgagt gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt
    32581 tagggataac agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt
    32641 aaacacctgg tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga
    32701 aagtacccat ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt
    32761 cgcttgtttc tttattgatc aaacggaagg ttacaaaaat tggtgctgag tagctagcat
    32821 cgtggatacg agcttcttct agcgtatatt ttggttcctt gatttcatat ccaacaaatt
    32881 ccaactccat tgtgtctgtg aagtttgaaa ttggcaatac atcttcaaac acttccttaa
    32941 gaccgtggtc taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt
    33001 caagaacttc tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt
    33061 catgtcctgc caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt
    33121 ttctaatcgt catatggttg taaacccctt atcaccgtgt cctcttgacg aattttcaga
    33181 atctttaagc ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct
    33241 aaatctgact ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac
    33301 aaaacctacg acaaatgatt acccatatta taccctattt agctagattt ttcaaggggt
    33361 tttagtaggt ttttggtaaa ttttttccca tagaaaactt ggcatcacat tcgaatcacg
    33421 ctatggtaca aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata
    33481 aacgttaagg cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt
    33541 tcaaatccgt ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc
    33601 caatttgaag ggcttttttt attttccctc gaataaatac gtataacttt aaaaactttt
    33661 ggagcgagtt tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa
    33721 aaagcttcgt cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc
    33781 gacttctgtg atttggactt ttaccttggt aacacgtcca ttcttcactt tatcattggt
    33841 taagataaac tgtttgtttt ggctaaccaa ttcatagctg agtttctcag tcgttggaat
    33901 agttcccact cctgtcaaat aataaccagc gatggtatca acgtcatcac tttccagttc
    33961 aacatcaaag taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc
    34021 ctcgccaatc tgatgaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac
    34081 aatttcctct aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag
    34141 caagattgcc atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat
    34201 agtttcaggt acaaaaagtg gatcttgcaa aattctcttc caaacaatat tgtcaaaacc
    34261 gtccacaaag cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt
    34321 atccccatca taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc
    34381 ttgactatca tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg
    34441 tgccatcagt tcatcgagcg aaaagacacc ttgtagcatc tcaatctcat cagcatccaa
    34501 tgtttcttca ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc
    34561 atcatcgaat gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca
    34621 aacaaaagga ctgactagtt ttccgatccc aatgataacc ggcgctatac gaattgccaa
    34681 ggcatccttt agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat
    34741 ataggtcaaa aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc
    34801 aagccaagag gcaatcacac gtcctagagt attagttaaa ctcgcccctg ataagattgt
    34861 aatcagggtg attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt
    34921 cagcaggcgg atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt
    34981 aagagaaacc atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa
    35041 caataaaaca aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct
    35101 aaaatagtat cttatccttt attatatcac aaaatttaat ccaatacata ttattttttc
    35161 ttaccgtaac ttctcaaagt aacttccatc tctctcccaa aactggaagt tagttttaga
    35221 agttgctatt ttttcttagt acctgttcta gccctgctta aactagtaaa aagaactcct
    35281 acatccataa gtgacatagg agtttaggct tatcttttac tgagttacag ttacttctcc
    35341 agttcggtaa tccagttgaa gtcccttttg aacattggga actagaacat tgaattccaa
    35401 ttctccatcc gaagacttgg cttcaatctg tgaagctgag ggaactaaat cctcgtttga
    35461 agcgtagtaa agggttacac ggtaacggac acgcttgttt tggtccaagg ctttacgcac
    35521 cttgctttca tagtagtttt gaccagtcga atactcggct tgtgcctgat ttgcccaggc
    35581 tgtctgaaca gcaatgtttt taggattgct tgttgaggca tcaaaaccat ccaaaccacc
    35641 gattaaggca tagcctaaca aatgacctct atcgactgca tgggtataag agccctttag
    35701 attcttgacc tgatgccaac ctggaggagt ccaagaagtt gaaccattcc cagtttcttt
    35761 acgattcttg tactgacgag tggccttaga caagagggca ttagctacgg ttggaacagt
    35821 ttccttgccc actgtctttg ttttattgtc agcgtagggc ttacttgaaa ccttggcatc
    35881 tagatttgtt ttattaccat tgacgataaa agcacctgag ccattccact ccagactccc
    35941 ctttatttga ctcttgactg cgtctgttaa gacactctct gccaatgctt gactaggagc
    36001 ttcagacgct tgttttttct gactaagatt ggttttggga ctattaggtg ccgacggcat
    36061 ctgcttgata taatagctcc ctgtagacaa aagcaataac actagcagtc cgattagtgt
    36121 ctgtcttgtt tttttgttca tatttctcct tatctttaga aaaaggctgg ttctccagcc
    36181 taatttcctg taaatttatg aatcaattcc tgccattttg ctggagacag gatagcccat
    36241 ggatcttgac ccttgcccaa gattccataa cctaccatta aaccgattcc tagagccaga
    36301 gtacctaaaa tcagtactat gatgactaaa agtaaacgct tgactacgta gcttgatttc
    36361 ttattcatct tcatcacttg cctcaatccg ctgaatctta gcacctagct gcgccaactt
    36421 ctcatggaaa ccgtagtaac ctctatccaa gtgaaccaat ttaccgacca cagtttctcc
    36481 ctgtgctacc aaacctgtca aaatcaaggc tgcactggca cgaaggtcag ttgaaagaac
    36541 ttctgctccc tgcaaaggct gtccaccaac aatacgagct gtatcacgga taatctcaga
    36601 atgcaagccc atgcggcgca tctcttctag gtgttggaaa cgattttcga aaactgtctc
    36661 caccatggtt gattcgcctt ttgcaactgt catcaaggct gtaaattgag cctgcatatc
    36721 tgttggaaat cctgggtggg gcaaggtttt cacatgaaca gcttttagat tttctagttg
    36781 agaacgaaca cgaattcctt cgtcttcttc aattacttca acacccattt caagtaactt
    36841 ggcaatcaag ggacggttgt gctcccagac agcgtctcga atcaagacat caccaccagt
    36901 catggcagca gctaccataa aggttcctgc ttcgatacgg tcttggacta cattgtgagt
    36961 cgtaccatga agtttctcaa caccagtaat ggttatagtc tctgtaccag cacctttgac
    37021 cttggctccc atttcattaa ggagaatggc taagtcaaca atctcaggct cacgcgcagc
    37081 attctcaatc actgtcaccc catcagccag agtcgctgcc atcatcaagt tctgcgttgc
    37141 accaacactt ggaaagtcca tatagatatg agcgccatgc aagcgttctg ccttggcttc
    37201 gatgtaacca gctgtctgac taatcttaac ccccatagct tccagacctt tcaaatgaag
    37261 atcaatagga cggctaccaa tcgtacaacc acctggcatg gataccttgg catgacccac
    37321 acgggcaagg attggcccta atacaacgat ggaggcgcgc atcttgctga catacttgta
    37381 aggggcttcc tcagtgatgt cgccagtagc atccaccttg acaagatgag cttcctcatc
    37441 aaagtcaacc ttggcattca aaccaccaac tacctgattc ataataaaga catccgacaa
    37501 aatcggaaca ttctgcaaga cggtctttcc ttcacttgct agaatagtcg ctgccaacaa
    37561 gggtaagact gcattttttg ctccctcgat cgtcacgctt cctaccagac gattatcgcc
    37621 accttgaacc acaattttat ccatactcgt ttcctttact cttgatttca taataatcct
    37681 ctaagcgctt cttgccacaa atgatacagg ctaataaaga aagaactcag gatgtagcct
    37741 atgacaatgc taaaaaaggc tactaataaa cgaacttttc ttgtattctc atctgtcact
    37801 tttaaaacct tctcccatct aacaagatcc tttaaaaggt aaaaactcac ataaataaag
    37861 agcatatgac tacttagagt gaataataat tgaaccattt gactattata ccatcttctc
    37921 tgtttgtgcg ctagtttgga aacttcactc aaaaattagg gattgttttt caagtaatca
    37981 atcgcatctt gtagggtttt aacgggcacg attttcatat ccgtcttgat tgttttagcc
    38041 gcttctaggg ctgtttggta gttgtttttc gcgtccggat gcgccttttg ttcttcttcg
    38101 ctaacagggt tatcaggagc aaagaaaata gcagcacctg ccctagccga agctacaacc
    38161 ttcttatcaa tacctccaat gtctcccaca ttaccatcgc ggtcaatggt acctgtaccg
    38221 gcaacaatac gaccattacg aagatctggg tgagctattt gagtatagat agctagacta
    38281 aacatgagac cagcacttgg accgccaata ccagctgttg aaaagctaat tgggacattg
    38341 ctgattacct ctgtacggtc aatcaagccg attccaattc catttttgcc attttccaag
    38401 gtgataatct ttccttctgc agacttggtt tgcccatcct cttcataggt gaccttgacg
    38461 gaatccccta atttttgaga actgacgtaa tcaatcaagt ctttggaact atcaaaggtc
    38521 tgatcattga ctgctgtgac tgtatcagag atattgagaa tccctttaaa ggttgaatta
    38581 tccgtcacat tcaaaacata aactccaaag tacttgagtt cgatatcctt accagctgtt
    38641 tttagtcctt gatacttggc catattttgc gatgtttgca tgtagaattg attgattcgc
    38701 ataaattcaa catcggaaga accacctgta gtctcctgag cactacgaat atctgtaaaa
    38761 ggtgtcaacc aagcataaat catatgagct aaagtggcat gttgaacacc aaccgtaacg
    38821 aattgatagg caccagcttc cttatcttct gtgtcattta ttttaaggac ttggcgaata
    38881 tcttccgaac cacctggaac ctctatataa taaggcaagg gcactacaaa tgccaagaaa
    38941 gtcacaatca aggccgcaat gacgtataag ggccatctaa tctttttttt catttcttat
    39001 ttcctccaaa atactctcgg gaacatagca ggcaatatcc cgaccaaact tcaaaagctc
    39061 tctaacgcct gatgaactga tatagagatg ttcaggtcga ctatgtaaat aaatagtctc
    39121 tatatcagaa gacagctgat gattgtagta atcaaaactg gcttcatatt gcaaatccga
    39181 cgcattcctc aaaccacgca ctagacaagt agcacccaat ctttttgcaa catcgaccac
    39241 caattcatca tgagaagcca cgacttcaac attttccaaa tgtcccaaag ccttttctag
    39301 cccccgttta cgattttcaa gagggagaaa tccttgtttg tggggattaa aaaaaatacc
    39361 cacataaagc ttatcaaaaa gtctgctcgc ccgttcaatg atatccagat gcccatttgt
    39421 catcggatca aatgagcctg tgaataagcc aatcttatct gacatagact gtcaccttac
    39481 taattccata aatcttttcc ttccagatac ccagacaggc aatttcttct ggaagttcaa
    39541 cggctttatc cgtctcgcac acaaccataa catcttcaga aaaaagctct ctctcagcca
    39601 ttttttcaat atctgctacg atttgttcct tggcataggg agggtctaag aaaacgaggt
    39661 caaattcccc agatacctgt tccaatgccc tttctgcatc catctttagg agttgaaatt
    39721 ttccaacttc cttggtcatc tggatatttt cagccacgat ggtctgagcc ttacggtctc
    39781 gctccaccaa aacagcactg gacatgccac gcgatactgc ttcgatagat aaaccaccac
    39841 tacctgcata aaggtccaag actcgtccca cttcaaagta gggaccaatc atgttaaaaa
    39901 tggctcccct aaccttatcc gaagtaggtc ttgtcgtctt gccttctagt gtcttgaggg
    39961 gacgtccccc atagattcct gatacgattt tcatactgtt tattatacca aattatagac
    40021 aaaaagagaa agaaaaccga accttgcggt tcgattctct acaaaatatt ttcgtaagta
    40081 tcgcggactt cttgaggcca aacacttgtt tgcacttctc cgatgtgtct cttgcgaagt
    40141 aggaacatgg ccatacgaga ttgtccaatt cctccaccga ttgtcaatgg gaatagacca
    40201 ttcaacaaag acttgtgcca ttccaattcc aagcggtctt catcacctgt aatttccacc
    40261 tgacgtctaa gagtttcttc atctacacga attcccatag aagacaactc aaaggctcca
    40321 cctaaagact cattccagac aagaatatca ccatttagac ccttgtagcc attctcagac
    40381 tcgcttgtcc agtcatcata gtctggtgca cgtccatcgt gcggtttacc atctggcaac
    40441 tcgccaccga taccaatcaa aaagacggct ccaaattctt tacaaatcgc attttcacgt
    40501 tctttcggtg tcaagtctgg gtagcgttct accaattctt ctgtatggat aaaggtgatt
    40561 tgttttggca agatagactc gatgtcatag cgggcttcaa cagctagctc agtcaggcga
    40621 atagccttat aaatcttctc aactgtttct tttagataaa cgatgttacg cttaccattt
    40681 gggataacct tctcccagtc ccactggtca acataaacag agtgggttgc atccaaggaa
    40741 tcctcatctg gacgaagggc tttcatgtgg acaaagagac cctctccttc accaaagcca
    40801 aaacgagcca aggtgtggcg tttccattta gcaagtgagt gcaccacttc ataagtagca
    40861 tcagggattt ggagaacctt gaccgatact ggattttcca caccagacag gttgtcctgc
    40921 attccgtcac cgaccttact caagatagga ccttgaactt cgacaacttc tagcttatct
    40981 ttcaaatact gggtaaaagt gtttttgaca aaggaaattt cttcttgttg atggataaaa
    41041 cttttcttca tagacaactc ctcaaaaatt taataaacgt attatacacc tatttccaag
    41101 aaaagaaaag agaaaattta aaaaaaaatc agtgtcactc aaaacactga tttcatagac
    41161 cttttagtca ttgcgaccaa agatacgtag aatacttagg aaaagattga taaaatcaag
    41221 atagatgctg agagccattg acaccaccca gcctgtcgct acacgacctt gtgattgttc
    41281 ataggcgagg cgaatctttt ggttatccca agcaatcaac cctgaaaaaa ccaaaaccat
    41341 ggctacgcta atcatataat caaagaagcc actagccaag aaaatattga ctaccatagc
    41401 aatcagtaaa ccaatgagag ctgccatcat tgctcgacca atcccactca agtctttctt
    41461 ggtaaaaata ccaactgccg ccatgacaaa gaagagaagg gcgctcgata caaaggcaga
    41521 taaaactgta cccggagtat agaaagccac cacaaaacta agggtaaagc cgtttaaaac
    41581 ggagtaaagt aaaaatactg gaagagcagc tggactattt cttgaagcca tgctactggc
    41641 aacgaaaacc aaagccaact ccgcaaaagt agcaatggtc aaccagagac gcccctgcat
    41701 caaaaagtaa accaactgag actgaaagac cgtcaacata aggcctgata ccaaagcaga
    41761 tagtccgatt cccagaccaa caaaggcata aaccttagcg taaaattgat tgagacctgc
    41821 acggtcatgt ataatagtgt gattcatcca tttctccttt tctgtgaaca tctttccttg
    41881 attataacaa aaaaagttaa aattagctta aatggaagat taaaataccg gctgcaaccg
    41941 caacattcaa actctcggcc tgccccttca tgctaatatg gaccaactgg tctgcacttt
    42001 cagccataag gggactaatt ccttgaccct catttcccat aactagtaca aaattttcta
    42061 tagaaggcag ttctctgtaa tcaacagaat ctttagatag ggttgttgct agcactggga
    42121 tagctacctt tttagtttcg tcaagaagcg cttgactagt catccggtaa atgggcagat
    42181 ggaaatgact accttgcatg gaacgtaggg ttttgagact gtagatgtct gccgacttat
    42241 ctgaaacaat cactcctgta aaacctgctg catccgcagt tcgaatgata gttcctacat
    42301 taccaggatc ttgcacatct tccaaaaaca agaacttgcc ctgactcaaa tcagcttgtc
    42361 ctacttcttc tttttgaacc acggcaacaa ttccctgtgg agtctgagaa tccgccaaat
    42421 ctagcaaaat atcctctgac acccagacag tttgcgaaaa agtagctaac tgatctcgat
    42481 aattttctag ggcaaagatt ttctcaatcg tcactccagc ttggacagct tcttcaaaca
    42541 agtgccagcc ttcaatcaag taggcagact tgcggtattt tttttggcgt aatttcttgg
    42601 cattttttac cacagaattg gcttttgagg ttataatagt catagaaata ttataacaca
    42661 atcaaagggg tttggtatgc aaaaggttag aatgattgcc caaggtaggg tacagggagt
    42721 cggctttcgt tggggtgttt acagcttggc acttgaaatt ggtggcatca caggtcgagt
    42781 atggaataac gacgatggca cagtggaaat cttagcccaa gcagactcat ctgctatcat
    42841 ggcaaaattt atccaagaaa tccgaaaagg accgacacct ttttcaaaag taagctactt
    42901 agatgtcaaa ctaagcaact ttcctcccta ctctgacttt aaaatcgcaa attaggtctc
    42961 cagaactatt gtatattttg taaaaaaaca gtagaataga aaggtataat ttttaaagaa
    43021 ggaataaaaa cagtgaaatc tattaaacgt tttgcactct cagctatggg agcggctatg
    43081 ttgcttgtct tgactggttg tgtcaatgtc gataaaacca caggtcagcc aacaggattt
    43141 atttggaata ccatcggagc ccctatggct gaagctatca agtacttcgc tactgataaa
    43201 ggtctaggct ttggtgtcgc tatcatcatc gtaaccatta tcgtgcgctt gattatcttg
    43261 ccacttggta tctaccaatc atggaaggca acgcttcact ctgaaaagat gaacgccctc
    43321 aagcacgtcc ttgagccaca ccaaacgcgt ctcaaagaag cgactactca agaagaaaaa
    43381 ctcgaagccc aacaagctct ctttgctgct caaaaagagc acggtatcag catgtttggc
    43441 ggtgtaggat gtttccctat cctccttcaa atgcctttct tctctgctat ctactttgct
    43501 gcccaacata ctgaaggggt tgctcaagca agctacctag gcattcctct aggttctcca
    43561 agtatgattt tggttgcctg tgctggtgtc ctttactatc ttcaatcgct cctttcactt
    43621 cacggagtag aagatgaaat gcaaagagaa caaatcaaga aaatgattta catgagccca
    43681 ctcatgatcg tcgtcttctc cctcttctca ccagctagtg tcacacttta ctgggttgtc
    43741 ggtggtttca tgatgattct ccaacagttt atcgtcaact atatcgttcg tccaaaactt
    43801 cgcaaaaaag tccgtgaaga actagccaag aacccaccaa aagcaagtgc tttctctaaa
    43861 ccaagtggac gaaaagacgt tacccctgaa caaccaactg ctatcacaag caagaaaaaa
    43921 cacaaaaatc gcaacgctgg aaaacaacgt tcgagataag aagaaaaagg cttagctgat
    43981 ttgctaagtc tttttgcaaa tcaaaagccc gatgtttcca tcaggcttct tttctatcca
    44041 tgtacacgtt tcatataatc ttgataactt tcggtatcca tgagtttttt agcgttcttg
    44101 acgcgatctg ctgttggtgg tttgactcct tcgagggaat atggaattcc aagttcacgc
    44161 cacttgaact cacccatggt gtgataaggt agaatttcaa acttatcaac atttttgagg
    44221 gtcttgacga acttaccaag ttcaatcaag tcatcatctc tgtctgtcaa tcctggaact
    44281 agcacgtggc gaatccagac aggttttcca atatctgata gatactgggc acaagccaag
    44341 atatttttat tggtttgtct agtgacaatc ttgtgctgtt cttcgttgat ttccttgata
    44401 tccaaaagaa ccaagtcagt gacagccatg agtttgtcaa acttctcaag gtaacgtggt
    44461 ttattacgga aaggaagagc acaggtatcc aaggtacagt ggattccttg ttccttagcc
    44521 ttggtgaaga gagcaatcag gaaatcaatc tgcaagagag cttctcctcc actgactgta
    44581 atcccaccct tatttcccca gaaaccacgg tagcgcaagg cctctgtcaa gacatcatct
    44641 accgtccgtt cacgtgactt attggactcc atagcccaag tgtctgggtt gtggcaatac
    44701 tggcaacgca tgtgacagcc ctgcaaaaag acaataaagc gaataccagg cccatctact
    44761 gacccaaagc tttctgtcga atgcaccatt cctgtcactt gtccataatc aattgtttct
    44821 tcagacatat cgttaccttc cttgaaaacg ttttatagtt ttattatatc acgacttgaa
    44881 ggaaaaacaa gatttttgcc tattttttag aaagcaatct gtttttcaat ttcattttca
    44941 atctattatc agattattct tcaaaatcga agccatataa ggttgcaaaa tagtcctcag
    45001 ggcgctctgc acggcggatt tgacgggctt taccttcttc ggtatagagg atttccgcag
    45061 aacgtaattt ggcattatac tggtagccca ttgaaaatcc gtgggcacct gtatcatgaa
    45121 ttaccagcaa atcaccgatt tctgtatgag gcagttcgcg attgactgca aatttatcat
    45181 tgttttcaca gagtgaacca accacatcta ccgcttcagc tggtccatct ggatgggtca
    45241 cgttgctaat atgatggtaa gctccgtaca tagctggacg catgaggttg actgctgagg
    45301 catccacacc tagataggta cggtaggttt ccttcttatg agtcactctt gtgactagag
    45361 caccgtgagg tgccagcata aaacgaccca attcggtgaa aatcttgacc tgaccaagac
    45421 ctgctgacgt aagaacttct tcatacacct tacgaactcc ctcaccaatc aaggcgatat
    45481 cgttcggctc ctggtctgga cgataattaa caccaatacc gccagaaaga ttgataaagt
    45541 ctagccaaat gcccaacttt tccttgattt caacagccag ttcaaagagc tgacgagcca
    45601 actctggata atagagatgg gtcacggtat tggacgctag gaaggagtga atcccaaaag
    45661 tcttggctcc tttttccttc aagatagcaa aggcttcaaa gagctggtcc ttggtcatgc
    45721 caaacttagc ctccccagga ttgtccataa tgtctgtccc cagttcaaaa acgcctccag
    45781 gattataacg acaagagatg atttctggaa tgcctgctac tctctccaga tgttcaatat
    45841 cttcaaaggc atccaagtta atggtcgcac ccaattcacg tgcataggcg tattccttgt
    45901 ctggcgtgtt gttggaagag aacataatct cagaacccag aaagtccagt ttatggctca
    45961 tcaaaagctc tacataacta gagcagtcca caccacaacc ttcttcttgg agaattttca
    46021 aaatagctgg agttggagta gccttaactg caaaatattc cttaaagccc ttgttccacg
    46081 aaaaagcttg gttgacggct cttgccttct cacgaatccc cttctcatca tacaagtgaa
    46141 agggagtcgg gaactcggca acaatcgctt ctaactcttc tctattgata aatggtgttt
    46201 tcataagctt ccttctattc tgtttgcaaa gaaaggctgg gacaatcgtt ccaaccttta
    46261 gttctatctc ttatttatct tcgtcaaaga tattgccaac ctcaacatcg atggcttggt
    46321 tcttgacatc aatattagtt accttcaaga gtgacttgta gtcaaactgc ttttcacgtt
    46381 cttcttgggc attgtccgca aagaccgcta cacctgccag ttctgagtcg aactcgcgca
    46441 agagactaat cataccgttg accgttccgc cacctttcaa gaagtcatcc acaatcaaga
    46501 cacggctacc tgccttaaga ctacgttttg aaaggaacat tttctcgata cggtcaccac
    46561 ttgaacctga aacatagttg acgctaacag ttgaaccttc ggtaattttc aggtcacggc
    46621 gcacaatgac aaaagagaca ttgaggacat tggcaactgc atttgcaagt ggcacaccct
    46681 tagttgctac ggtcataacc gcgtcaattt tttggtccat aaagcttttg gcaataatac
    46741 gaccaatatt tttcaagatg gctggtgtgc taagcaaatc agacagatag atataaccac
    46801 ctggcaagat acggtcactt tctgacaact tggtacgcaa gtcttcaacc atttccttag
    46861 catcctgact cgaaatagac ggtgtgaaga tgacacctcc gccagcccca gtcactgtct
    46921 ggatatgacc gatttcaatt tcctcaaagg cgcgtttgat aatgacgata tcttctgaga
    46981 tggatgattt agcagactca tacttttcag caaaagtatt gagactagtt agtttataag
    47041 gattattaat caaatagttg gaaatgacaa ccatccgatc acttcttctt aatttcattt
    47101 ctatttccta ttccattttt attcaaaaaa tcaaaaagca aactagaaag ctggtcgctg
    47161 gtggttcaaa acactgtttt gagattgtca atagaactga caaaccctgt aatatacctg
    47221 catatataca tacgacaagg cgatactacc ctagtttgaa gagattttcg aagagtattc
    47281 atttttgtct tttacttatt ataccatatt cacataaaaa aacgaacatt cttatcctaa
    47341 aaaatgctca tttttcttaa attattaatc taaatctggt ttatagaagg aacgattatc
    47401 catagcgaag attttattgg tcatctctcc tttatccacc aaagcaagag ctgttgacat
    47461 catcatcatg cttgcatcca gattgtcaat catatggata atctctgctt ccataatgcg
    47521 tggacggact gggcttccat actcaagcaa gccgtggtga ctgaggatga catgacgaag
    47581 caaaacgact tcttccttgg tatcatcgat gccgagttcc ataactgtct tggtaatttc
    47641 gctatcaatg agagcgatat gtccaagaag attacctcgc actgtgtact ctgtctggtc
    47701 tggccccgtc aactcgatga ccttagctaa gtcatgcaac ataatccccg catagagcag
    47761 gctcttattg agctgaggat aaacttcgct aatagcgtct gccaaacgca ccatggtcgc
    47821 cgtatgatag gccaagcccg tttcaaaggc atggtggttg gtcttggcag ctggatagga
    47881 gtagaattcc ttatcatact tggtgtagag atttcggaca atccgttgcc agacaggatt
    47941 ttcaattttg aaaatcattt gcgacatgta gtcacgaatt tccttgacat caactggtga
    48001 cttgaccttg aaatcggctg ggtcattggg ttcaccagct tgaggcaggc ggagagtaat
    48061 ttgattgact tgaggggtat tgttataaac ttctcggcgt cctttcatgt ggacaacctt
    48121 acctgcggta aaggcctcaa tgttatgagg ttgggcatcc cagagcttcc catcaatctc
    48181 gccactatca tcttggaagg taaaggctag gtagtttttc ccagctcgag tttgcctcag
    48241 gtcagctgat ttgattaggt aaaagccttc aaatagctca tcttttttca tgtgactaat
    48301 cttcatattc ttcctcattt tcttgaaaat ggagtagatc aagcgcaggc tcgccttctg
    48361 acaactcaat gtgacggagc gtccgctcga tagctatggt acgacggttt aataattcat
    48421 caatattgcc agaggcatgt tggagatgtt tttgtgcctt gaccagaata ccaccaaact
    48481 tgccaaactc ggtcttgaca ctggcaagag tcttgctgat atggtcggca ctcttttgga
    48541 tattaagggt cttgaaacca actgataggg agttaagaag ggctgatagg gtacttggtc
    48601 ctgcaacgat aatctgttct tcccgtctca aatcatcaaa gaagaccgga ttgcgcacaa
    48661 tctctgaata aagaccttct gtcggaacaa acaaaactcc aaaattggtc gtccgaggcg
    48721 gtgctatgta cttgctcttg atatccttgg caaagcgctt gacgcttgct aggagtgact
    48781 tacgacagcg ttcaatctca tccttgtcac ctgtctcata ggcttcttcc aagcggtaat
    48841 aatctgccag tggaaactta gagtcaattg gcagatagac gtattcttgg tcgccttgtc
    48901 cgggtaactt gatggcatac tccactcgtt cactagagtt ttcaaccgtt gcgtattctc
    48961 gttcgtactg ggcaggtgtc atgatgtctt caataatttg ccccagttgc aattctccca
    49021 gaatccctcg cgtcttggtt ccagagagaa ccttgttaag agctccgaca tcacgggcaa
    49081 ctgtctgcat ttctccaagg ccacgattga cagactccag ttgtttagaa actgtctcaa
    49141 aggaagcctg taagcgtgtc tgcaaggtct tttctagttt ttcctcgacc gtctggcgca
    49201 tttgttccaa acgttgctca tttgattcct gcaaggcttg gagacgttgg tctgtcttgt
    49261 ctctagtttg gaggagatta tctgtcattt cttgacggac ttgggtcaga ccttggtgca
    49321 attcaatccg cacttcttgc aaacggtcgc tgacaaccac ttccaaatct ttttggtcta
    49381 actggctggc ttgtctggct tggtcaaagc ggtaatccaa ctggtctgac aaatgatctg
    49441 cctgatcctc caagctctta cttaagtgtt tctcctgcct atcctgcctt tgccaaatca
    49501 gaaagagacc agctaggttg gcaattaata atagaataag taaactttcc atcctacctc
    49561 ctgtccttgc tatgcagtac gaccacataa ccatctgggc aagttaccga cacttcccta
    49621 tctatatatt cgttagaagc gtacactttt ttaaagaaaa aattttcctc tgtcaactca
    49681 tacttggctc caagaatagt cagctggcta tcccgaactg gcataaaggc tagatagtcg
    49741 tagtctgaac gaggttctag ctgactggtc ccttctggac aataagtaat caagttttgc
    49801 ccatcctcaa tttctatttg atgcatatag ggtgccaact taggattgct aggcagaaag
    49861 acattggcca acatatggtc aatacggcca cccaaggcac cgaaaatagt aacctgagcc
    49921 tgaggatttt tctcaaagat ggttaagaga gccaattcca aatcggtatc atctttttct
    49981 ggtcgggctt ggacaaaatg ctgggcacgt ttttgaatca cctgtcgctc ttcttcggtc
    50041 acagaatcaa aatccccaac tgctagagca agaagcaagt cttcttccaa gacccagagc
    50101 gagcctcgat ccaccccaac aaaagcatca aaatctgtcc gataatgacc gcggtctccg
    50161 cctgcaaaaa cggcaaccct agtccagttg ttttctgaga gtttgtactc gctcattgac
    50221 atctccctta aagacatagg aacctgctac aaaaacggtc gcaccagctt ctttggcttg
    50281 ggcaatagtt tggtcatcaa tcccaccatc cacttcgatt tcaaagttca aacctttttc
    50341 ctcacgaaga gcaaccaact cacgaacctt atccatggtt tctggcagaa aggcttgccc
    50401 accaaaacct ggattaaccg tcatgactaa aacttggtca actagatgaa ggacgtgctt
    50461 gatggcttca actgatgtgc caggattgat aacgactgaa ggcttaactc cgagtgaacg
    50521 aattttttgg agggcgccat gaatatgagg cgttgcttct acatggatac tgatgatgtc
    50581 tgcacctgca cgcgcaaaat cttccagatg atgctcaggg tttgacacca tcaagtggca
    50641 atcgaaaacc atcttactat gaggacgaag gctctcgacc acacctgcac caaaactgat
    50701 ttgcggtaca aaatgactgt ccatgatatc gatatgggca tattctgccc cagttgcttc
    50761 tagacgtttg atttcacgtt caaagttggc ataatctgct gccagaattg acggagcaat
    50821 cttgtattga gacataggtt tctccttatt ttggaatttt tttgctgact tttttatagg
    50881 tttctctacg attttcaatt tcactaagga attgcaggta attgtcaaaa cggaaggttg
    50941 caataacacc ctcttcaaca gctggtttga cggcacaaga cggctcatgg gtatgggtac
    51001 aagtacggaa cttacaatct cggctaacag tagcaatctc tggaaaggcc tgattgaggt
    51061 cttcagccct tgatacttca tagtccagag atgaaaagcc tggtgtatct gcgattttac
    51121 ccccgttgag attgtaaaaa ctaacagctc gagtggtatg gcgaccgcga cctagactgt
    51181 ctgaaatttc tcccgtttca agattgaggt ctggtgcgat tttattgaga agagttgact
    51241 tcccaacacc tgtctgcccc ataaagaccg taaccttgcc tgttaacaaa gacaggagtt
    51301 cctctttact ggtcacaaag tcatagccga tgtcaccata ggtctgctgg taaaaatcca
    51361 gttctcccct atcttccaac aaatccattt tggaaatata gacaatggga tggatgccct
    51421 tgtgctccaa aagaaccaag aaacgatcca gcaaattgct gttaaaatca ggttccttga
    51481 cggacatgat tactacagct tgatcgatat tgacaatagg cggacgaacc agactgtttt
    51541 tccgttcgtg aattttgagg atatagcctt ctgaattttc ctcggcagag aaatctaccc
    51601 agtccccaac ataaggggta tggccttttt tacggaaatt cccacgcgcg cgtgtttgat
    51661 aaacctggcc atcactctcc acatagtaga aacctgccaa ggctttaatg atttgtccct
    51721 gcatcttaga gtccttgccc tttaagtgcg tctgctagac cggcaaattc tgccaagctg
    51781 agagcttccc cacgcacact tggtgacaag cctgcctggt ccaaagcctt ggtcagcttg
    51841 tccttgacct cttcagtctt accaaagtaa cctgtcaagt tattccacaa ggtcttgcgg
    51901 cgatgggtaa aactagcctt ggaaacctta aagaaaaagt tctcatcttc tactgctacg
    51961 gctggctctg gacgacgcac cattttcaag atggctgaat ccacatttgg cgctggcaca
    52021 aagaccgtac gaggcacgat aaaggcaacc ttggctgtca tgtaatactg cacggcgata
    52081 gacaagctac cgtaagcctt ggtgttaggc tgggctgaaa tgcggtccgc tacttctttc
    52141 tgcatcatga ccacaaactc acaaaaagga atgccactct caatcaagtg catgagaata
    52201 ggcgtcgtga tgtagtaagg caaattagcc actaccttga ttggcaggtt aggattttta
    52261 aaattttgga tatgttgcgc caaatcaacc ttgagaatat cttcgttaac tacggtcaca
    52321 ttatcaaaat cacgcagagt atctgccaaa attggcatca aacggtggtc aatctcaaaa
    52381 gccatgactt gggctgcacg ctcagccaaa aattctgtca aggcaccaat acctggcccg
    52441 atttcgatga cattgacctg atcatcaatt tcagccgtat ccacaatttt ttgaaggata
    52501 ttggtatccg tcaagaaatt ttgcccaaag gactttttaa aggtaaaacc gtgacgctcc
    52561 aacactgcct tggtcacgct ataatctgca attctcatct attctctttt ctattatctg
    52621 ttactactat tgtaacacat tccccttgta ttttgggaat gaaaatcata ccaaaaaact
    52681 atctttcctc atgaagaaag atagtcttgg aaatctttaa agagggactt agtgcttctc
    52741 tcgaaacata cgttcatact cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc
    52801 gtactcaagt acagcctgcg gctagcttcc tagtttgctc tttgattttc attgagtatc
    52861 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
    52921 cacatcatcc actgtcagtt caaccgtctg aggtaaattt ttcaaacgat aatgcttttg
    52981 cctttgccct tcaatactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
    53041 aaggagatga ttggtcatat tttccatgat gaggatatct tctaaaccac ctctggcact
    53101 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
    53161 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
    53221 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
    53281 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gtcttagcaa
    53341 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
    53401 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
    53461 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
    53521 cgaaattttg aaagagatag cccatctggt ctcgaaaata ctcacgagta ggaatggttt
    53581 ttaaatcttt cccctgatag agaacccttc caccatctat cttttctagc tttcccaaga
    53641 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
    53701 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
    53761 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
    53821 ttatcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
    53881 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
    53941 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
    54001 atcataagtt cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
    54061 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc cacccgtttg
    54121 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
    54181 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
    54241 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
    54301 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
    54361 aggaaacgtt cctgtcctgt ttctgtaaaa gctagcctta cctgtgggag gtagtgtttc
    54421 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatcct
    54481 tggtagtagg cagactgctc tcgcaagctc tcaggcaaga tcagcccaaa ctctccctca
    54541 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
    54601 tagcgcggcg agacatagat aacattccct aacggtgtgt agtcactgag acgattgcca
    54661 tctaggtcca cttctgctcc atctgagaaa ttgtcaacat tgctcataat ataaaaagag
    54721 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
    54781 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
    54841 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
    54901 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
    54961 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
    55021 ccaagtagat agacgacact tagtaaggta gaaatccctg ccaaggtcaa tccataaagt
    55081 agaagagcaa tgatgaccag ttgtaccgtt gccataaaca aggcaccttg ataccagaga
    55141 atccccaatc ccaaaagact ggataccagc actgagcaga taagctgtct cacatcttct
    55201 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
    55261 tgacgaaggg atttgatccg ataaatcagg gtcagactca taaaggtcag cagaaaaata
    55321 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
    55381 tctccattcg aaacaaagcc ttgataacca agctctttca aggtggtctg taagctcact
    55441 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
    55501 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
    55561 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
    55621 tctaccgcca agtgactcaa agcctctgtc acctcttcgc gactagctac tggttgattt
    55681 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
    55741 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
    55801 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accaagtaaa
    55861 gctaaacaat agggctatac cattctaaac attatagtag aatgcaactt tctctcccca
    55921 agaagtacga atccatgcac gagaagtccc tccagcacca gcatatccct tgtcagatga
    55981 accagttttt ctgctaacaa ctgttgaact atggtatttt gaagggtgat agtagtttga
    56041 gtaagcacca ccgttacctg aaccataagt ccaaacacca ccttctgggt attgaacggc
    56101 tgcagaggca actgatacac ctccacctaa tgttgctgca atgctcgcta caagagctaa
    56161 ctttttaact gtttgtttca tattgatact cctctatttt tgattacaag attgttgagt
    56221 tacttgctcc tctttccatt atcaacgttg atactattat attaccaaat gatattctaa
    56281 aattatttgt ttttctctaa attgtcaaaa tagcaccctg aaatgaactt ttatagtcat
    56341 tatactccat ctttatcaca ctatcaagga catcaatgtc cttaaaatct aactatttca
    56401 tattattttt agcttatatc taaaaaaaca gttctaaaga cgaactttag aactgttttg
    56461 atacgaggac aaggacattt ttgtcactga ttcactatgt ttataaatat tttttgacat
    56521 cctcttgcca ttcctctgtc aatttcttta ctagaaatcc atctccaatt agcttcgcaa
    56581 atgttttaga agactgataa aatgattcag ctaaactaaa atctctttct actcttaatg
    56641 cttgcttcca caaaaacata aatacaagag gtttcttttg ataatcttga gttttatcca
    56701 taatttcttg taaacacgat agataatcat taaaacaatc agttactcct accttttcaa
    56761 gacaacacat tcctgaaata agagtatctc taattataaa gaagttttct aatttagtta
    56821 cctttttctg ttccaaaaga aagagcatta gcttttcgat ttgatttata aatttggcaa
    56881 gatcttttct aatgagcatt tgataaaaaa acaaacgaat caacaacata tcatttgttg
    56941 aataaacttc tttttctatg atctgagaca tatgttcctc aagtattaac tctgcatatt
    57001 caggccttct agttctagaa gtatccaaac acgcctgcaa tacttcacat gctaattgtt
    57061 cttctttagg aagattatca aagtatgttt tataaatctc ttccaaacac gcttctttct
    57121 tatcatactc ctcttcttta ccatagattg gttcacgtaa aatctggtat ttcaattcta
    57181 aataagcaga aggaagagct gaaaaatccg gcataaggct ataaactgga actcctagcc
    57241 ggcgagcaat ataggctaac ttgggcaaac tcggttgcga agctccactt tcaattcgcg
    57301 acagttgacg aactgtcagt tcttgctcat ctccacaaaa atctggacga ctaattcctt
    57361 tttctatccg aatctcttta attttttgac caattaacat cagacgcctc ctcttctacc
    57421 tgcttcgaaa aatactgtga aacttaaaaa attgtaacgg ctacatcttt cctactcata
    57481 agatttcata gcttcttcca cttctgccaa agtaacccca aacaactcta ggcgtttgag
    57541 gagttgcttg ccattagaat agccgattcg gagagtctct ccgagatatt ctctacgctt
    57601 acggctgtct gcccctgcta gaaaaccaag gcgaatcaag tcgctacgac taatgtcaaa
    57661 ctgactctca tgttcaaatt gttctgtcac ctgagctaga gccgttttca ggtcttcata
    57721 gctggcatgc tcaattccca gagaacgccc cttggtcttg gacttgggaa cagcttcatc
    57781 tcgcttgaga aaggcatgct gaactgttgg aatggccgtc ataatcatgc gccgaatccg
    57841 ctccccatta aaatctgggt ctgtaaagac aatgactcca tgacgttggt gtagacgctg
    57901 aattcgctct atatcctgat cattgatggc agaacctcgt gtctcatagg tctccacatc
    57961 gaaataacgc ttgagattga ccgtatcata gcgaccttca accacgataa cttgagaaat
    58021 tttctctttc attacttgct gtccaatcca aaaattcgtt ctgcatttgc agtcgttgct
    58081 accgccagct cttctgtcgt cataccacgc aagtcagcga taaagtcgac cacatagcga
    58141 gtataggctg ttttattttc acgaccacgc ttgggtacag gtgctaagta aggcgcatct
    58201 gtttccacca acatcttgtc caaaggtaac tctttagctg cttcttggag gtcagttgcc
    58261 ttcttgaaag tcaccactcc tgagaaggaa atggtcatac caagatccac aaacttctct
    58321 gcccactcaa gcgtccctga aaatgaatgc atgataccac cacgaggacc aacgccctca
    58381 ctcttgataa tctcataggt atcttccagc gcatcacggg tatggacaac aaaaggcaaa
    58441 tccaagtcct tagatagctg aatctgacgg cgaaaaacct gctcctgcac ctctttgggc
    58501 gctgtcatcc aatggtagtc taagccaatt tcacctaaag ccacaacctt ggaatgtttt
    58561 aacttatcca acaagtaagc ctcaacttcc tctgtataag taccagcttc tgtaggatgc
    58621 caaccaatag tcgcatagag ctgctcatac tcatctacca actccaaggc acgctcaatc
    58681 gtcggtttat caaaaccaac aatattcatc tgtatcacac ccatctcagc agccaaggca
    58741 atttcttctg cctcacgacc tgcaaattct tctacattca agtgtgtatg tgtatcaaaa
    58801 atcatctctt ctaacctcgt tttctatctt ctattatacc aaaaaagagg aggggcacct
    58861 aatttttcgg tttcccctcc tctcttcaat agagagctat tctgctatct tttctatccg
    58921 atattgccca tctcctattc cacagttaga gacagaagag attggctaca ttgatagaat
    58981 attcctattc agctaaagta tgtgattatc ttgggttctc acttttctca cccatcacta
    59041 ggattgatga cttctcaaaa tcccccttat aatctaactt taagtagaga atagaatttt
    59101 ctagctgttc tattttctac gtctagtctt tcttcctagc aaagcaaaac cacttgttgc
    59161 caccactaga ccaagactag caagtgctgc acttgatgct gaacctgtgt taggtaattg
    59221 tttgccagca ggtgactggt aggtaacatc ttgcattgtt cctgcagttg cttgcggtgc
    59281 acttgttgtc ttaggagcag cattaggagc cactggtctc ttaggaactg taaccatggc
    59341 aatggcttgc actccaatcg tttctgcttc cttcacatct tccatagttt tcgcattttc
    59401 aatctctttg agagctgctt gtttttctgc ttccactctt gctaaaagtt ctgctttttc
    59461 tttatcagaa agcggtgcgc ctttgatttc atcctgctta tccttggctg ctttttcaat
    59521 ggcattttta gctgattctt tttcagtagc taattgctta gaagcttgtg attgagctga
    59581 gttttgtgaa cctgtgtgag ctgatccacc cgcctggcta ccaccgcctt cattcgcatt
    59641 agcatctgag cttccagtat tgcctccctt atctccacct ggtatagttt ctagcttata
    59701 ctctagtgta gcttcttgag ttacagattc acctttcgca gattgttgaa ctgtatcttt
    59761 tcctagaatc gtcactactg aaccatctgg gaatgtgatt gttgctgtac catctccagc
    59821 tacattgatt gtcgctccgt ctaatgctga accatttgct tgtaaaatag caaccttaac
    59881 tttttcttca tctgttaaat gagttatatc ttttacatct actctttgag caggtgcttt
    59941 aacagttgat ttttcagcag tcggtttagt gctatcttgt gcttttggat cttgtgctac
    60001 caattgattt cctggaattg taatagctga accatcttta aagacgattg ttgctgtacc
    60061 atctccacct acttcaactt tatagtaaaa taaaataata acaggacaaa tcgatcagga
    60121 cagtcaaatc gatttctaac aatgttttag aaacagaggt gtactattct agtttcaatc
    60181 tactataaca tcgcttccga atccatttgc atctactgaa cttttttaca aaaataaaaa
    60241 tcagtagact cgagttccta ctgatttttg atagaattcg tttggattat gcagccaaaa
    60301 ctttgcgtcc tttacgacga cgagctgcca atacgcgacg accattttta gttgacatac
    60361 ggttacggaa tccgtgttta cgcgcacgac gaagtttact tggttgataa gtacgtttca
    60421 cgatgaatac ctcctcatag attttgtatt cgtttagccg gctataaagt gatcagttac
    60481 taaacatact ttactattct atctgattct ttcacttttg tcaatagctc taggcaaatg
    60541 tttccagctt tttcgaatgc aagccctatt gaaagttcta taatgaagtt agccacctta
    60601 ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt catttgataa
    60661 caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt ttagctgaaa
    60721 aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata agaaaacacc
    60781 tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat gcaagaacat
    60841 tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga gcggtggaag
    60901 aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc tcaaacgatt
    60961 cata
//
LOCUS       DBERYS010000008        53321 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.008, whole genome shotgun sequence.
ACCESSION   DBERYS010000008 DBERYS010000000
VERSION     DBERYS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 53321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.008"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..136)
                     /locus_tag="ACL8LA_000567"
     CDS             complement(<1..136)
                     /locus_tag="ACL8LA_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1812378.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     H"
     gene            complement(382..701)
                     /locus_tag="ACL8LA_000568"
                     /pseudo
     CDS             complement(382..701)
                     /locus_tag="ACL8LA_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
     gene            870..1412
                     /locus_tag="ACL8LA_000569"
     CDS             870..1412
                     /locus_tag="ACL8LA_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGS1812379.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            1412..2041
                     /gene="nth"
                     /locus_tag="ACL8LA_000570"
     CDS             1412..2041
                     /gene="nth"
                     /locus_tag="ACL8LA_000570"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGS1812380.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            2252..2773
                     /gene="pyrR"
                     /locus_tag="ACL8LA_000571"
     CDS             2252..2773
                     /gene="pyrR"
                     /locus_tag="ACL8LA_000571"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGS1812381.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2792..3715
                     /locus_tag="ACL8LA_000572"
     CDS             2792..3715
                     /locus_tag="ACL8LA_000572"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGS1812382.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            3765..4844
                     /locus_tag="ACL8LA_000573"
     CDS             3765..4844
                     /locus_tag="ACL8LA_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGS1812383.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            5156..8332
                     /gene="carB"
                     /locus_tag="ACL8LA_000574"
     CDS             5156..8332
                     /gene="carB"
                     /locus_tag="ACL8LA_000574"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGS1812384.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(8607..9416)
                     /locus_tag="ACL8LA_000575"
     CDS             complement(8607..9416)
                     /locus_tag="ACL8LA_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1812385.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9418..10221)
                     /locus_tag="ACL8LA_000576"
     CDS             complement(9418..10221)
                     /locus_tag="ACL8LA_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1812386.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(10231..11718)
                     /gene="tacF"
                     /locus_tag="ACL8LA_000577"
     CDS             complement(10231..11718)
                     /gene="tacF"
                     /locus_tag="ACL8LA_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGS1812387.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            12054..12761
                     /locus_tag="ACL8LA_000578"
     CDS             12054..12761
                     /locus_tag="ACL8LA_000578"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGS1812388.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            12766..13788
                     /locus_tag="ACL8LA_000579"
     CDS             12766..13788
                     /locus_tag="ACL8LA_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGS1812389.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            13805..14674
                     /gene="licA"
                     /locus_tag="ACL8LA_000580"
     CDS             13805..14674
                     /gene="licA"
                     /locus_tag="ACL8LA_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGS1812390.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            14664..15542
                     /locus_tag="ACL8LA_000581"
     CDS             14664..15542
                     /locus_tag="ACL8LA_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1812391.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            15554..16243
                     /locus_tag="ACL8LA_000582"
     CDS             15554..16243
                     /locus_tag="ACL8LA_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGS1812392.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            16339..17187
                     /gene="dprA"
                     /locus_tag="ACL8LA_000583"
     CDS             16339..17187
                     /gene="dprA"
                     /locus_tag="ACL8LA_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGS1812393.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            18076..19119
                     /locus_tag="ACL8LA_000584"
     CDS             18076..19119
                     /locus_tag="ACL8LA_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGS1812394.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            19286..21373
                     /gene="topA"
                     /locus_tag="ACL8LA_000585"
     CDS             19286..21373
                     /gene="topA"
                     /locus_tag="ACL8LA_000585"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGS1812395.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            21479..21838
                     /locus_tag="ACL8LA_000586"
     CDS             21479..21838
                     /locus_tag="ACL8LA_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGS1812396.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            21931..22563
                     /locus_tag="ACL8LA_000587"
     CDS             21931..22563
                     /locus_tag="ACL8LA_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGS1812397.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            22607..23263
                     /locus_tag="ACL8LA_000588"
     CDS             22607..23263
                     /locus_tag="ACL8LA_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGS1812398.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            23333..24376
                     /locus_tag="ACL8LA_000589"
                     /pseudo
     CDS             23333..24376
                     /locus_tag="ACL8LA_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            24388..25425
                     /gene="leuB"
                     /locus_tag="ACL8LA_000590"
                     /pseudo
     CDS             24388..25425
                     /gene="leuB"
                     /locus_tag="ACL8LA_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            25422..25691
                     /locus_tag="ACL8LA_000591"
     CDS             25422..25691
                     /locus_tag="ACL8LA_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGS1812399.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            25695..26054
                     /gene="leuD"
                     /locus_tag="ACL8LA_000592"
     CDS             25695..26054
                     /gene="leuD"
                     /locus_tag="ACL8LA_000592"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGS1812400.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            26216..26839
                     /locus_tag="ACL8LA_000593"
                     /pseudo
     CDS             26216..26839
                     /locus_tag="ACL8LA_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899133.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            27005..28936
                     /locus_tag="ACL8LA_000594"
     CDS             27005..28936
                     /locus_tag="ACL8LA_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGS1812401.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSGVSGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            28923..30239
                     /locus_tag="ACL8LA_000595"
     CDS             28923..30239
                     /locus_tag="ACL8LA_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGS1812402.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFFHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            30414..31400
                     /locus_tag="ACL8LA_000596"
     CDS             30414..31400
                     /locus_tag="ACL8LA_000596"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGS1812403.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCVKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            31464..31535
                     /locus_tag="ACL8LA_000597"
     tRNA            31464..31535
                     /locus_tag="ACL8LA_000597"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31497..31499,aa:Arg,seq:ccg)
     gene            31718..32416
                     /gene="rnc"
                     /locus_tag="ACL8LA_000598"
     CDS             31718..32416
                     /gene="rnc"
                     /locus_tag="ACL8LA_000598"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGS1812404.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            32407..35946
                     /gene="smc"
                     /locus_tag="ACL8LA_000599"
     CDS             32407..35946
                     /gene="smc"
                     /locus_tag="ACL8LA_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGS1812405.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSHQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            35943..36737
                     /locus_tag="ACL8LA_000600"
     CDS             35943..36737
                     /locus_tag="ACL8LA_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1812406.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            36737..37555
                     /locus_tag="ACL8LA_000601"
     CDS             36737..37555
                     /locus_tag="ACL8LA_000601"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1812407.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            37559..38836
                     /gene="ftsY"
                     /locus_tag="ACL8LA_000602"
     CDS             37559..38836
                     /gene="ftsY"
                     /locus_tag="ACL8LA_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGS1812408.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVIEVSQKEVKEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(38882..40369)
                     /gene="zwf"
                     /locus_tag="ACL8LA_000603"
     CDS             complement(38882..40369)
                     /gene="zwf"
                     /locus_tag="ACL8LA_000603"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGS1812409.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWD
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(40518..41258)
                     /locus_tag="ACL8LA_000604"
     CDS             complement(40518..41258)
                     /locus_tag="ACL8LA_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1812410.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(41258..43423)
                     /locus_tag="ACL8LA_000605"
     CDS             complement(41258..43423)
                     /locus_tag="ACL8LA_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS1812411.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            43596..44549
                     /locus_tag="ACL8LA_000606"
     CDS             43596..44549
                     /locus_tag="ACL8LA_000606"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGS1812412.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLQQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSSLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            44626..46614
                     /gene="uvrB"
                     /locus_tag="ACL8LA_000607"
     CDS             44626..46614
                     /gene="uvrB"
                     /locus_tag="ACL8LA_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGS1812413.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            46624..47187
                     /locus_tag="ACL8LA_000608"
     CDS             46624..47187
                     /locus_tag="ACL8LA_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1812414.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            47153..47641
                     /locus_tag="ACL8LA_000609"
     CDS             47153..47641
                     /locus_tag="ACL8LA_000609"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812415.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            47715..48182
                     /locus_tag="ACL8LA_000610"
     CDS             47715..48182
                     /locus_tag="ACL8LA_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS1812416.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(48179..48694)
                     /locus_tag="ACL8LA_000611"
     CDS             complement(48179..48694)
                     /locus_tag="ACL8LA_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGS1812417.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(48706..49233)
                     /locus_tag="ACL8LA_000612"
     CDS             complement(48706..49233)
                     /locus_tag="ACL8LA_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1812418.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(49511..50074)
                     /locus_tag="ACL8LA_000613"
     CDS             complement(49511..50074)
                     /locus_tag="ACL8LA_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1812419.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(50058..50609)
                     /gene="coaC"
                     /locus_tag="ACL8LA_000614"
     CDS             complement(50058..50609)
                     /gene="coaC"
                     /locus_tag="ACL8LA_000614"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGS1812420.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(50621..51310)
                     /gene="coaB"
                     /locus_tag="ACL8LA_000615"
     CDS             complement(50621..51310)
                     /gene="coaB"
                     /locus_tag="ACL8LA_000615"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGS1812421.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            51540..53210
                     /locus_tag="ACL8LA_000616"
     CDS             51540..53210
                     /locus_tag="ACL8LA_000616"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGS1812422.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIYVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT        16375 a         9579 c        11624 g        15743 t
ORIGIN      
        1 tatgaatcgt ttgaggcgcc tttcctaaca agcctgcaat ttcacgattg gacttatttt
       61 cattcttcca ccgctcaatc aagcgacggt tatctattgt caaatgtttc ccttttgggg
      121 tataatgttc ttgcatctct gtgcctttcc ttgtgtttgt ggtgaacaac aagtatagca
      181 cagaggtgtt ttcttatacc ttaaatcaca tttcatttga caacaggaac ccttgaacct
      241 gttttttcag ctaaaccaag gctatgatta gccttggcca cctagcctaa cagtttcaaa
      301 cctgttatca aatgaaatca tcaacttact caaacatagt gaaacatcta attcttgact
      361 aaggtaaggt ggctaacttc attatagaac tttcaaaaca ggtccataga gacttagtac
      421 gtgtttgaca tactcgtaat ggattttatc atagtttcgc caagcagtgc gtgcttgatt
      481 gtttagtttt aggctaccca gtccaatcag aggactggtg ggttggtaaa atttctcaag
      541 attaattaag atactgttgt tcaacttttc tgttttttga gctcttgcat agtagtgttt
      601 agcagctctt gtagtcgaat atcatctgcc gtaacctgtt tacagttttg gtagctttta
      661 tttaacttgg ataggagttg aatgagtttc ttgtcttcca tgtgagcatc ctccttgatc
      721 tgatgtactc tttccctata gtatagcact aaaaaataaa actatcatag taaatatgtt
      781 aaaatttgaa ttttaattac aaagtttggt gtgctgattt gtagcctttt catggtataa
      841 tcaagagagt aaatctttat ggaggaagta tgaaattaaa tattcaagaa attcgtaagc
      901 agtctgaagg tttgaacttt gaacaaacgt tagatttagt tgatgacctg cgtgcacgta
      961 atcaagaaat tttagatgta aaagatatcc ttgcagttgg gaaagtacaa tatgaagacc
     1021 gtatgtattt cttagattat caactatctt ataccattgt tcttgcttcg agtcgcagta
     1081 tggagccagt tgagttagtt gaatcttatc cagtcacgga agttttcatg gaaggcgcaa
     1141 ctaaccagct agatcaagaa gttttagatg atgacttggt cttgcccatc gaaaatgggg
     1201 cgcttgacct tgctgagagt gtatcagaca atatcctgct aaacattcct atcaaggtct
     1261 tgacggctga agaagaagct ggtcaaggat ttgtctcagg aaatgactgg caaatcatga
     1321 cagaggaaga ataccaagct caacaagcag taaagaaaga agaaaacagt ccttttgctg
     1381 gcttacaagg actatttgac ggagatgaat aatggtcttg tcaaaaaaac gagcacgaaa
     1441 ggtgctagaa gaaatcattg ccctcttccc agatgccaag cctagtcttg attttaccaa
     1501 tcattttgaa ctcctggttg cggtcatgtt atcagcccag acgacagatg cagcggtaaa
     1561 taaggccaca ccaggtctct ttgttgcctt tccaacaccc caagctatgt ctgtagcgac
     1621 agagagtgag attgcttcac atatttctcg cttgggattg tatcggaata aagctaaatt
     1681 ccttaaaaaa tgtgcccaac agttactaga cgattttgat ggtcaagtcc ctcagacacg
     1741 tgaagaattg gagagtttgg caggtgttgg tcgcaagaca gccaatgttg tcatgagtgt
     1801 aggatttggg attccagcct ttgcagtgga tactcatgtg gagcgtattt gcaaacacca
     1861 cgatattgtc aaaaaatcag cgacgccact tgaggtggaa aagcgggtca tggatatctt
     1921 gccgcctgag cagtggttag ctgcccatca ggccatgatt tattttggaa gagccatttg
     1981 ccatccaaaa aatccagagt gtgaccagta cccacaatta tatgatttta gcaatttgta
     2041 agatgctaca ttgaaacttt ttgcttgatt ttaagcatgc tttgtgctat agtaaatgat
     2101 aactaaatat tcctttaaac ctgtcccgtg aggcaggcaa ggagcgcgat aaagtagaag
     2161 ggtgaagtct acactgtgtg cagtagggct cgcttggcta taaatttcaa gtctccttgt
     2221 ttaaggagac ttttcttttt ggaggtttgt catgaaaaca aaagaagttg tagacgaatt
     2281 gaccgtcaaa cgagcgatta cgcgtattac ttatgagatt atcgaacgca acaaagattt
     2341 gaataagatc gtcttggctg gtattaaaac tcgtggtgtc tttattgccc accgaatcca
     2401 agaacgtttg aagcagttag aaaatctttc agttcctgtt gtggaattgg atactaaacc
     2461 tttccgtgat gatgttaaaa gtggagaaga tacttctttg gtttctgtcg atgtgacaga
     2521 ccgcgaagtt atcttggtgg atgatgtgct ctatacaggt cgtaccatcc gtgctgctat
     2581 tgataatatt gtcggtcatg gtcgtcctgc gcgtgtgagt ttagcagttc tagtcgatcg
     2641 tggacataga gaattgccaa tccgtccaga ttacgttgga aaaaatatcc caaccagtcg
     2701 ttctgaagaa atcatcgtag agatgacaga acttgatgac caagacagag ttctgattac
     2761 tgaagaagct tagaaagtta aaggagtagc catgtcagaa aatcaacaag cattgaacca
     2821 tgtggtgtcc atggaagacc tcactgtcga tcaagtaatg aaattgatca agcgaggaat
     2881 tgagtttaaa aatggagccc agcttcccta tgaagaccat ccgattgttt ccaatctctt
     2941 ctttgaggat tctacacgga cacataagtc ctttgaagtc gcagagatta aacttggatt
     3001 ggaacgactt gactttgatg tgaagactag ttcggttaat aagggtgaga cactttatga
     3061 caccattttg actctgtctg ctttaggagt ggatgtctgt gtgattcgcc atccagaggt
     3121 cgactactat agagaattga ttgctagtcc aacgattacg acttccatca tcaatggtgg
     3181 agatggttcg ggccaacacc ctagccagag cttgcttgat ttgatgacca tttatgagga
     3241 atttggccac tttgagggtc ttaaagttgc tattgcaggt gacttggatc actcacgcgt
     3301 tgccaaatcc aatatgcaga ttttgaaacg cttgggagct gaactctttt tcgctggacc
     3361 tgaggaatgg agaagtcaag agtttgcaga ctatggacag tttgtaacca ttgatgaaat
     3421 cattgatcag gttgatgtca tgatgtttct ccgtgtgcaa cacgaacgcc atgatagtgg
     3481 agctgtattt tcaaaagaag actaccatgc ccaacatggc ttgactcaag aacgttacga
     3541 tcgtttgaaa gaaacagcaa tcctcatgca cccagctcca atcaatcgtg atgtagaaat
     3601 agcagaccac ttggttgaag caccaaaatc acggattgtc caacaaatga ccaatggtgt
     3661 ctttgttcga atggcaatct tagaatccgt actagcgagt agaaacgcca actaaaacac
     3721 aagacgttaa aagacgccag tgagcgtcca cgagaggtga aaatatgaca aaaagaattc
     3781 tagtattaga agatggcaca gtttttgaag gcaaggcctt cggagcagat attgatgtaa
     3841 caggcgaaat cgtctttaat acagggatga ccggctacca agaatccatt acagaccagt
     3901 cttataatgg acaaatcttg acctttactt atcctttggt aggaaattat ggtattaatc
     3961 gtgatgatta tgaatccatt attccaactt gtaagggagt cgttgttttt gaagaagcgc
     4021 gtagagctag caactggcgc aaccaaatga ccttggatga atttttgaaa gccaagaaaa
     4081 ttccagggat ttcaggaatt gatacgcgtg ctcttaccaa gattatccgt aagcatggta
     4141 ctatgcgtgc aaccttgacc catgttgggg acagtatgga ccatgtgacg gaccagctcc
     4201 aagcaacagt tttaccgaca gacaatatca aacaggtttc tactaaaacg tcttatccag
     4261 ctccaggagt tggtttgagc gtggtgctag tggactttgg tctcaagcac tcaatcctac
     4321 gtgaactttc taaacgcaac tgtaacgtga cagttgttcc ttattcgaca acggctgaag
     4381 aaattctcca tctcaatcct gatggagtta tgttgtcaaa tggtccaggt aacccagaag
     4441 acgttccaca agcactcgac atgattcgcg gtgtacaagg aaaaattcca atctttggta
     4501 tttgtatggg acaccaactc tttgcaatgg caaacggggc taagacctat aagatgaagt
     4561 ttggccatcg tggattcaac catgcggtac gtgaaatcgc aacaggacga gtagacttca
     4621 ccagccagaa ccatggttat gcagtcagcc gcgaggattt gccagagcac ttgattatta
     4681 cccacgaaga aatcaatgac aagtcagtgg aaggtgtgcg ccacagatac caacctgctt
     4741 tctctgttca ataccaccca gatgcagctc ctgggccaca cgacgctagc tacctatttg
     4801 acgagtttat cgagatgatg gaagttttta aacaatcaaa ctaagaacga gtaaaagctc
     4861 gtcggagaat ttatgtaact gaacacggac ggaaagctca gaatttagag aaaccaactt
     4921 ggattgtcaa tccttccttg atttcttaaa attcaatcgc tttctattcg tcctttgtat
     4981 cttatttaat gtcgccctga gaggcgacga atagaaagga gaagatacat tgttcgcgga
     5041 agtgaacacg ccctaagaac tgtgtgaaaa agataaacat cgtcttgacg ctgaaggcgt
     5101 ctgcgccgag tttcctattt tcactgtgtt ctttacgggc tttgtatcat aaactatgcc
     5161 taaacgtact gatattcaaa aaattatggt gattggttct ggtccgatta ttattggtca
     5221 ggctgctgag tttgactacg ctgggaccca ggcttgcttg tcgttgaaag aggaaggtta
     5281 tgaggttgtt ttggttaact caaatcctgc aaccatcatg acggacaagg aaattgctga
     5341 caaggtttac attgaaccga ttacacttga gtttgtgaca cgtattcttc gtaaggaacg
     5401 tccagatgcc ttgctaccaa cactcggtgg tcagacaggt ctcaacatgg ccatggaatt
     5461 atctaaaaat ggtatcctag acgagcttgg cgttgaactt ctgggtacca aattatctgc
     5521 cattgaccaa gcggaggacc gtgacctctt taaacaattg atggaagagc ttgagcaacc
     5581 cattccagaa tctgaaattg ttaacacagt agaagaagca gttgcctttg cagcgacaat
     5641 tggctaccca gtcatcgttc gtccagcctt tacccttggt ggtactggtg gtggtatgtg
     5701 tgccaacgaa aaagaattgc gtgaaatcac tgaaaatggt ttgaaattgt cacctgttac
     5761 ccaatgtttg attgagcgtt cgattgccgg ttttaaggaa attgaatacg aagtgatgcg
     5821 tgactcagct gacaatgcct tggttgtttg taacatggaa aactttgacc cagttgggat
     5881 tcacacaggg gattccattg tatttgcccc tgcgcaaacc atgtcagact atgaaaacca
     5941 aatgcttcgt gatgcgagct tgagcattat tcgcgccctc aagattgaag gtggatgtaa
     6001 tgttcagcta gctcttgatc ctaacagctt caagtattat gttatcgaag taaaccctcg
     6061 tgtatcacgt tcgtcagccc ttgcttctaa agcgacagga tatccgattg ctaagttggc
     6121 tgccaagatt gcagtaggtt tgaccttgga tgaggttata aacccagtta caggttcaac
     6181 ctatgccatg tttgaaccag cccttgacta cgtagttgct aagattccac gtttcccatt
     6241 tgacaagttt gaaaagggtg aacgccgtct cggtacacaa atgaaggcga ctggagaagt
     6301 catggcaatc ggtcgtaaca tcgaggaatc tcttctcaag gcctgccgct cccttgaaat
     6361 tggggtgcac cacaatgaaa tacctgaact tgcagcagtt tctgatgatg ccttgattga
     6421 aaaggttgtc aaagcccaag atgaccgtct cttctacgta tcagaagcca ttcgtcgtgg
     6481 ctacacacct gaagaaattg cagagcttac aaaaattgat atcttctatc ttgataaact
     6541 cttgcatatc tttgaaattg agcaagaatt gggtgctcat ccacaagatc tagaagtttt
     6601 gaaaacagcc aaacttaatg gtttctcaga ccgtaagatt gctgaactct ggggaacgac
     6661 tgatgatcaa gttcgccaac ttcgcttgga aaacaagatt gttccagttt ataagatggt
     6721 agatacttgt gcggcagagt tcgactctga aacgccatat ttctattcaa cctatggttg
     6781 ggaaaatgaa tctatcagat ctgataaaga atctgtactt gtcctaggtt caggtccaat
     6841 ccgtatcggt caaggggttg agtttgacta cgcaaccgtt cactctgtta aggctatcca
     6901 ggcagctggt tatgaagcta tcatcatgaa ctcaaaccca gagaccgttt caacagactt
     6961 ctctgtatca gataagcttt actttgagcc attgacattc gaagatgtta tgaacgtcat
     7021 tgacttggaa caaccaaagg gagttatcgt tcagtttggt ggtcaaacag ccatcaacct
     7081 tgcggagcca ttggcaaaag caggtgtgac catccttggt acacaggttg ctgacctaga
     7141 ccgtgccgaa gaccgtgacc tcttcgagca agctctcaaa gaattggata ttccacagcc
     7201 accaggacaa acggctacca atgaagaaga agcagctctt gcagctcgta agattggctt
     7261 cccagttctt gtccgcccat cttatgtact tggtggacgt gccatggaaa tcgttgaaaa
     7321 cgaagaagac cttcgttctt acatgcgaac tgctgttaag gctagtccag atcacccagt
     7381 tcttgtcgac tcttatatcg ttgggcaaga gtgcgaagtt gatgccattt cagatggtaa
     7441 agatgtgctc attcctggta tcatggagca tatcgaacgt gctggtgtcc actcaggtga
     7501 ctcaatggct gtttacccac cacaaacctt gtcgcaaaag gttcaggaga ccatcgcaga
     7561 ctacaccaaa cgcttagcaa tcggtcttca ctgccttgga atgatgaaca tccagtttgt
     7621 tatcaaggat gaaaaagtct acgttattga ggtcaatcca cgtgccagcc gtactgttcc
     7681 attcctatct aaggtaacca atattcctat ggctcaagta gcgaccaagc tcattcttgg
     7741 tcaaagttta tcagaacttg gctaccaaaa tggactttac cctgaaagca cccgtgttca
     7801 tatcaaggca cctgtcttct cctttaccaa actagctaag gtagacagct tgctcggtcc
     7861 tgaaatgaag tcaacaggtg aagttatggg ttctgatgct actttggaaa aagctctcta
     7921 taaagccttt gaagcttctt atctacactt gccaactttt ggtaatgtcg tctttaccat
     7981 cgctgatgat gccaaagaag aagccttgaa cttggctcgt cgtttccaaa acattggtta
     8041 tggaatcctt gcgacagaag ggacagcagc cttctttgct agtcatggct tgcaagccca
     8101 acctgttggt aagattggtg atgacgataa ggatatccca agttttgtac gcaaaggaag
     8161 aattcaagct atcattaaca cagtaggaac aaaacgaact gctgacgaag atggtgagca
     8221 aattcgtcgt tcagccattg aacacggggt gcccctcttc acagcgctag atacagctaa
     8281 tgccatgctt aaggtacttg aaagtcgtag ctttgttaca gaagcgattt aaatagaaaa
     8341 ataagatata gcagactgaa tttagaacag tacacaggaa tactaaaata tttctataaa
     8401 ttaatttgaa ttttctaatt gatttgttcg catcttattt caatctacta tagaaaaaag
     8461 tctttaaaat tataaaacgc atcatatcaa ggtttttcaa aaaccttgat atgatgcgtt
     8521 ttattgtggg aatatgtatt tcattttcta ctaaaattat gtttttgaat aacctctatc
     8581 ttagtagttt gtataatccc cctcaatcag tttttgcgat aagctttaat gctatgacta
     8641 taccactctt gcatttcttt tgatggtggt gtcatataat cgccatacat ctgggttaaa
     8701 aattggtcat attttttggg aacaggcaac atacggccct caaactcagt taaaatcagt
     8761 tctttaaagg tatcaactgg gaagatttct ttcatccctt ccttaccgat cccaactcct
     8821 ccttcatatt gaggaatgtt ggttacagca tttttgacta gttgatcaat tttcttgtaa
     8881 aagtagcgag gattgacaaa tcggagagcg taccagctac ataatctaag aaaatctttt
     8941 agtttgctat caccgtgaac tgctcgtgat tttttgatat aagctagttg acgaagagcc
     9001 acatacttat agctcttgtc gacaatgctc aagtctgtaa atcgatcaat tgggaagaca
     9061 tcgatgaaaa ggctggtatc atgacgcttg tacttaacat ggtcttctat aacagtagaa
     9121 gtgtccaaaa tcgatgcgaa attatggaag taccaagaag atgtatcgta ggaaagaacc
     9181 ttgtagcgag ggtgattttc ttcttcaata atcttcagta aacgctcata atcctcacga
     9241 taaagggaaa tatcaatatc atcatcccaa ggaatcatac ctttgtggcg gatggctcca
     9301 agcatggttc cataactgag aaaataagga atatcatgtt tcttacaagt ctcatcaata
     9361 tagtccagca gggctagttg aatttcttta atttcttttt tttctaaata ttgcatccta
     9421 atcctccaat ttataagcgt gaaattcatg actgtagaag cgtttttctt ctggtggtag
     9481 ggtcatataa tctccataaa attgtgtcaa aatagtatca aatttttcag gtgcaggaag
     9541 gcttaaattc tcaaagggta aatcgattgt tttatcaaag gtaccacttg ggaagacttc
     9601 cttttcctta aattttgaag ggataaaagc catatactgc ccattttcac gactatattt
     9661 ttgaatttct ttctcgattt tatttgcaaa ataacgagga gaaactggtc gaaggagcaa
     9721 ccaaaaggct gttcgtatcc aatcttttaa aaggctatcc ttatagacaa tatttttatg
     9781 tttactgaaa gacagcagtt tgaagctttc cagtttataa caagtatcaa tgaccttagg
     9841 atcatcaaag cgatccatag ggaaaatatc gataaagata ccagactcat aggtttttgt
     9901 atttcgagta tcaataattt tagtcgtact gtcggttatc ttgataaagt tgttaaagta
     9961 gttcttatca gtttctaagg ataggagttc atacttgctt ttttcctttt gaaaaatgtt
    10021 aataaatcgt tggtagtctt ctctaggcat ggataaatca atatcgtcgt cccaagggat
    10081 aaagccctca tgtcgaaccg ccccaatcag agtaccgtag ttaataatat agttgatatt
    10141 gtgcttttta cagagagtat caatataatc caaaatttct aattcaattt gtttggcatc
    10201 ttcaacggtt agttgtttca ttttaaactc ctatgatttt ttaaatttat tttttaaggc
    10261 tagaacatgg tttaaaaatt catagaaaat gctatctttt gtgaagacaa gtagactaat
    10321 ataagagata gctgatagca agataatcaa accagtatta atcaaaaatg gcaaattaat
    10381 gaccatatcc acaggataca cgaaattaat caggaaataa attgctacaa aggaaagtga
    10441 aaagagagag tatcgaacag tatagctaaa gatatgtccc aagtggatga gttgttttct
    10501 atggatgaaa atgatataga aaacaagtag agaagcctct gataacatag ttgtcagtaa
    10561 gtagtattcg ggagccacga tacggttgaa aaagaggaga ctattcaagc ccaaattgag
    10621 tagcccagca aagactgtat aaactgtgat acgtttttca tagccattgg taaagagaat
    10681 ttgggaacca agaatggtat ctaaggccag gataatcgta cgaaaagcga agagagaggt
    10741 caagatgccg cctccgatat atttttcact accgtaaagt aggatggcat ttggtcctaa
    10801 aaccatgagt ccaaaactca gtggaatgat aaagaagtta aagattcgac tacctctatt
    10861 aaccagagaa acataggctt ctttgtctcc tttccccaga tagtaactga gacgaggcac
    10921 actcactcca attgcacctg ttacaacccc agctataacg gtcacaattc gctgagctat
    10981 ggtatagtaa ctaacgttga catcaatccc tgttttaacg aggaagaggc gatctaaaaa
    11041 agtgaagagc atattggcat tggcaaagac taacatggct gtcagaggga gaaagagtgg
    11101 tttaaaatca cttaggtgaa tcttaacaag tttgatgtct cttttaatcc aaaaataact
    11161 aatcaggtag ttaatcagcg tcgataaact catcacaagt gtatagacaa caatatcgtg
    11221 ttcattttta acaaataaga aaatagagac cagcatcagg atacggatga aggcagtttt
    11281 gtaaaagaga aaactgtaat tttccagagc ttcattgacc cattcgattg aaaaaatctg
    11341 ggcaatgagt tgaatcccca taacaaggta gacctttttg atgattggat tatcagtaaa
    11401 gaagagagga taggctagga tatagacagc agtggtcaaa atcgtacaag cgatgcacaa
    11461 ataaaaaaga ctagaaaagg ttctgttaag atcttttttg ttatccttga cattactgat
    11521 agcccttaaa ccgtagttat agacaccata agttgcaaag ggcaagaaaa atgacaaaat
    11581 agtgtcgact gagttgaagt aaccatagtc agttcggtcc aagacacgcg cgacataggt
    11641 tccagttagg atgggaaaaa taatattcaa gacacgaatt cccatgtaag atagagcatt
    11701 taattttata cttttcattc aatttacctc gtttttcatt atatcataaa gttagctaat
    11761 aagaaatgaa gggcagtaag ccaagcaatc actttgaagt ttcaaatctt aaattttaag
    11821 ttttctttaa ggaaagtata ttattctgaa ggactctaaa atttcgcagt catttattag
    11881 taattgctac agaattccta gtcattacta gaaatggact agtttctttg aataatagaa
    11941 ctgcataatt ctcctattct agaaggggag gaccagtatt tcttttatga taggactaga
    12001 ttgtggtata atagagagaa taagtttttt tagtaagaca aaggagaaaa tagatgattt
    12061 atgcaggaat tcttgccggt ggaactggca cacgcatggg gatcagtaac ttgccaaaac
    12121 aatttttaga gctaggtgat cgacctattt tgattcatac aattgaaaaa tttgtcttgg
    12181 agccaagtat tgaaaaaatt gtagttggtg ttcatggaga ctgggtttct catgcagaag
    12241 atcttgtaga taaatatctt cctctttata aggaacgtat catcattaca aagggtggtg
    12301 ctgaccgcaa tacaagtatt aagaaaatca ttgaagccat tgatgcttat cgtccgctta
    12361 ctccagagga tatcgttgtt acccacgatt ctgttcgtcc atttattaca cttcgcatga
    12421 ttcaggacaa tatccaactt gcccaaaatc atgacgcagt ggacacagtg gtagaagcag
    12481 ttgatactat cgttgaaagt accaatggtc aatttattac agatattcca aatcgtgctc
    12541 acctttatca aggacaaaca cctcaaacat tccgttgcaa ggacttcatg gacctttatg
    12601 gatctctttc tgatgaagag aaggaaatct tgacagatgc atgtaaaatc tttgtgatca
    12661 aaggaaaaga tgtggccttg gccaaaggtg aatactcaaa tctgaagatt acaaccgtaa
    12721 cagatttgaa gattgcaaaa agtatgattg agaaagacta gtaaaatgat taatcaaatt
    12781 tatcaactaa ctaagcctaa gtttatcaat gtcaaatatc aggaagaggc tattgaccaa
    12841 gagaatcata tccttatccg tcccaactac atggctgtct gtcatgcgga tcagcgttac
    12901 tatcagggaa aacgtgatcc caagattttg aataaaaagc ttccaatggc aatgattcac
    12961 gagtcatgtg gaaccgtcat ttctgacccg accggaacct acgaggttgg tcaaaaagtt
    13021 gtcatgattc ccaatcagtc tcctatgcag agtgatgaag aattctatga aaactacatg
    13081 acagggaccc atttcttgtc tagtggattt gatggcttta tgagagagtt tgtttctctc
    13141 cctaaagatc gtgtggtggc ttatgatgct attgaagata cggttgcagc cattacagag
    13201 tttgtcagtg tgggcatgca cgctatgaat cgtctattga ctcttgctca tagcaagcgg
    13261 gagcggatcg ccgttattgg agatggaagt ttagcttttg tggttgccaa tattatcaac
    13321 tatactttgc cagaatcaga gattgtggtt attggtcgtc attgggaaaa gttggaactc
    13381 ttctcatttg ccaaagaatg ctatattacg gataatattc ctgaagagtt ggcctttgac
    13441 catgcttttg aatgttgtgg tggtgatggt actggaccag ctattaatga cttgattcgc
    13501 tacattcgtc ctcagggaac gattctcatg atgggagtta gcgaatataa agtcaatctc
    13561 aatactcgcg atgccttaga aaagggcttg attttggttg ggtcatctcg ttctggtcgc
    13621 attgatttta aaaatgctat ccaaatgatg gaagtcaaga aatttgccaa tcgtcttaaa
    13681 aatatccttt atctagaaga acctgtaaga gaaattaaag atattcatcg tgtctttgca
    13741 accgatttaa acacagcctt taaaacagtg tttaagtggg aagtataagt actggaggtt
    13801 aattgtggag aaaatcatta aagaaaaaat ttcttcctta cttagtcaag aagaggaagt
    13861 cctcagtgtt gaacaactgg gtggaatgac caatcaaaac tatttggcca aaacaacaaa
    13921 taagcaatac attgttaaat tctttggtaa agggacagaa aagcttatca atcgacaaga
    13981 tgaaaagtac aatcttgaac tactaaagga tttaggctta gatgtaaaaa attatctttt
    14041 tgatattgaa gctggtatca aagtaaatga gtatatcgaa tctgcgatta cgcttgattc
    14101 aacgtcaatc aagaccaagt tcgacaaaat tgctccaata ttacaaacta ttcatacgtc
    14161 tgctaaggaa ttaagaggag aatttgctcc ttttgaagaa atcaaaaaat acgaatcctt
    14221 gattgaagaa caaattcctt atgccaacta tgaatctgtt agaaatgcag tcttctcctt
    14281 agagaaaaga ctggctgact taggtgttga cagaaaatct tgtcatatcg atttggtgcc
    14341 tgaaaacttt atcgaatcac ctcaaggacg actttatttg attgactggg aatattcatc
    14401 aatgaatgat ccaatgtggg atttggctgc cctcttttta gagtctgaat tcacttccca
    14461 agaggaagaa actttcttat ctcactatga gagtgaccaa acaccggttt ctcatgaaaa
    14521 gattgctatt tataaaattt tacaagatac tatttggagt ctatggactg tctataagga
    14581 agagcaaggt gaagattttg gtgactatgg tgtgaatcgt taccaaagag ctgttaaagg
    14641 tttggcttct tatggaggtt cagatgaaaa gtaaaaacgg agttcctttt ggccttctct
    14701 caggtatttt ctggggcttg ggtctaacgg ttagtgctta tatcttttcg atttttacag
    14761 atttgtcacc ctttgtggtg gctgcagctc atgatttttt gagcatcttt atcttactag
    14821 cttttctctt ggtaaaagaa gggaaagttc gcctctcaat tttcttaaat attcgcaatg
    14881 tcagtgttat catcggagcc ttgctagcag gccctatcgg tatgcaggcc aatctttatg
    14941 cagttaagta tatcggaagt tctttagctt catctgtatc ggctatttac cctgcgattt
    15001 cagttctatt ggctttcttc tttttgaagc acaagatttc gaaaaatact gtatttggga
    15061 ttgtcttgat tattggaggg attattgctc agacctataa ggttgaacag gttaattctt
    15121 tctacattgg gattctttgt gctttggttt gtgctattgc atggggaagt gagagtgttc
    15181 ttagctcttt tgccatggaa agtgaattga gtgaaatcga agccctctta atccgtcaag
    15241 taacttcgtt cttgtcctat cttgtgattg tgctcttctc tcatcagtca tttactgcag
    15301 tagccaatgg acaattgcta ggtctcatga ttgtttttgc agcctttgat atgatttcct
    15361 acttggctta ttatatcgct atcaatcgct tgcaaccagc caaggctaca ggcttgaacg
    15421 tgagctatgt agtatggacg gtcttgtttg cagttgtttt cttgggtgca ccgctagata
    15481 tgctgaccat tatgacgtca cttgtcgtca ttgctggagt ttatattatt attaaagaat
    15541 aaaggagatt cgtgtgaaag ccattatctt agcagcggga ttgggaactc gcttgcgtcc
    15601 tatgactgaa aataccccta aagccttggt tcaggttaat caaaaacctt tgattgagta
    15661 ccaaattgag tttctcaaag aaaaaggaat caatgacatc atcatcattg ttggttatct
    15721 taaagaacaa ttcgattact tgaaagagaa atacggtgtt cgtctcgttt tcaatgataa
    15781 atacgctgac tacaataact tttactctct ctatcttgta aaagaagaat tggccaacag
    15841 ctatgttatt gatgctgaca attatctctt taaaaatatg ttccgcaatg atttgacacg
    15901 ttcgacttat tttagtgttt atcgtgaaga ttgtaccaac gaatggttct tggtttatgg
    15961 agatgactac aaggttcaag acattattgt tgatagcaag gcaggtcgca tccttagtgg
    16021 tgtatccttc tgggatgctc caactgcaga aaagattgtc agctttatcg acaaggctta
    16081 tgtaagtggt gaatttgttg atctctattg ggacaatatg gttaaggata atatcaaaga
    16141 gctagatgtc tatgttgaag aattagaagg caatagcatt tatgagatcg atagtgtcca
    16201 agactatcgt aaattagaag aaattcttaa aaacgaaaat taaagattcc aacatctgac
    16261 aaaatagtcg gatgtttttt gattttttac gaacttttac gaatagatag atgagtagaa
    16321 aaagaaatgg agttatttat gaaaatcaca aactatgaaa tctataagtt aaaaaaatca
    16381 ggtttgacca atcaacagat tttgaaagtg ctagaatacg gtgaaaatgt tgatcaggag
    16441 cttttgttgg gtgatattgc agatatctca ggttgccgta atccagccgt ttttatggaa
    16501 cgttattttc agatagacga tgcgcatttg tcgaaagagt ttcaaaaatt tccatctttc
    16561 tctattttag atgactgtta tccttgggat ttgagtgaaa tatatgatgc gcctgtactt
    16621 ttattttaca agggaaatct tgacctcctg aaattcccga aggtagcggt cgtgggcagt
    16681 cgtgcttgta gcaaacaggg agctaagtca gttgaaaaag tcattcaagg cttggaaaat
    16741 gaactggtta ttgtcagtgg tctggccaag ggcattgaca cagcagctca tatggcagct
    16801 cttcagaatg gcggaaaaac cattgcagtg attggaacag gactggatgt gttttatcct
    16861 aaagccaata aacgcttgca agactacatc ggcaatgacc atctggttct aagtgaatat
    16921 ggacctggtg aacaacctct gaaatttcat tttcctgccc gtaatcgcat cattgctgga
    16981 ctttgtcgtg gtgtgattgt agcagaggct aagatgcgtt caggtagtct cattacgtgt
    17041 gagcgagcaa tggaagaagg acgcgatgtc tttgctattc ctggtagcat tttagatgga
    17101 ctatcagacg gttgccatca tttgattcaa gaaggagcaa aattagtcac cagtgggcaa
    17161 gatgttcttg cggaatttga attttaaaaa tgacctaagc tagaattcta agaaaaaatc
    17221 aattttaaga gaaaatgaac ccaacatttc cataataaaa cgcatattag caagttttta
    17281 acacttgata atatgcgttt tttctaagtg gattagtaga gtagaggatt tttctcatat
    17341 aatactcttc gaaaatctct tcaaactacg tcagcttcca tctgcaacct caaaacagta
    17401 ttttgagctg acttcgtcag tcttatctac aacctcaaag cagtgctttg agcaacctgt
    17461 ggctagcttc ctagtttgcg ctttgatttt cattgagtat aagggaaagt atagtgaatt
    17521 gaaataagat gtgaacaact ctatcaggaa agtcaaattg atttatagaa atattttagc
    17581 agccaaggtg cactgttata gattcaatac actataattt agtgtaattg agaaaggaga
    17641 aatgattgtg attgatgttg gctaggttat gttcaatgat tcctaccgtc tcaaatcttg
    17701 tcagtaagga aaaataaatt cttcaaaagt agagattaca aggcttgttt aagaaagaat
    17761 tcaaagacct tgacaaataa aaataaaatg gttattataa aaaatggtct gaaatagatg
    17821 atgatacttt tcgaaaatct cttcaaacta cgtcagctca gctttgcctt gctgtgtttt
    17881 gagcaagcta cggttagctt ccgagtttga ttttcattta ctagaaatga aactgatgag
    17941 agatatcagt agacatttga gtcaggatat tatggaaaat gataaaaaga gctcgtgaga
    18001 ttggcatatc agactactaa agtattgagt ttgttaggat tttagcgact agttagctgg
    18061 gaaaggaaga tatttgtgac aaataataaa ctgtattcgt tgatagaatt tagaaataaa
    18121 atatatgaag aattagaact ttccagaagt gatttagcga ttttactatg tgccatgctt
    18181 atcgcctcta tcggattaaa tatggattcg actcccgtga ttattggagc catgttaatc
    18241 tctcctttga tgacacctat tctgggagtg gggctctctc tagctatatt tgattttaaa
    18301 ttgttaagaa aatcttttaa aatattagct attcaaattc ttgccagtct aatagcttca
    18361 acactttatt tttatctttc tcccatttcg tatgctagtt cggagattgt tgctagaacc
    18421 tctccgacta tttgggatgt tctcattgct tttgtaggag ggatagcagg tattattggt
    18481 gctaggaaaa aagagaccaa taatattgtt cctggtgttg ctattgcaac cgccttgatg
    18541 cctcctcttt gtacagtagg ttatgctatt gcttctgcta atctaaaatt tatcataggc
    18601 tcctcttacc tattcctcat caattgtagc tttattgtca ttgcgactta tataggtgtt
    18661 aggttgatga tggttaagaa acattatttt aaagataatg aagaagactc taaaatgcgt
    18721 aggattttgc ttctagttgc tgttttgctg atgattccga gtttcatctc tgcaacgact
    18781 ttagtgagag aaacgttgaa aaaagagtcc cttaagaaat ttatatcaga gcagtttcag
    18841 gggtataata ttttgaaaaa aacctattct aaaaagactc ataccctaaa gctaaccatt
    18901 tcaggaaatt atttgacaga agaagaactc gatatgattt caagtaagag aggtgactat
    18961 ggtttaagtg atgtttctgt tcaagtttca caattgtctg attcagaaca acttagtaag
    19021 gaagagctgg tggagtattt cttccagtat atcaaggata aggaagcaaa agaaaaggag
    19081 aaagccaata aatttgatac agagtctgag gagcaataat ttcttgagaa tagctggttt
    19141 ctctcgtgag tcttctatgt atatcaaagg aagactgagg ttttaagtat aaaacttttc
    19201 ttctattata gtagctaagt cttgacaggg ttcaaaaaat ggtttacact ttataaagtt
    19261 tattactttg aaaaggtgtg atactgtggc tacggcaaca aaaaagaaaa aatcaacagt
    19321 taaaaaaaat ctagtcatcg tggagtcgcc tgctaaggcc aagacgattg aaaaatatct
    19381 aggcagaaac tacaaggttt tagccagtgt cgggcatatc cgtgatttga agaaatccag
    19441 tatgtccgtc gatattgaaa ataattatga accgcaatat attaatatcc gaggaaaagg
    19501 ccctcttatc aatgacttga aaaaagaagc taaaaaagct aataaagttt ttctcgcgag
    19561 tgacccggac cgtgaaggag aagcgatttc ttggcatttg gcccatattc tcaacttgga
    19621 tgaaaatgat gccaaccgtg tggtcttcaa tgaaatcacc aaggatgcag tcaaaaatgc
    19681 ttttaaagaa cctcgtaaga tcgatatgga cttggtcgat gcccaacaag ctcgtcggat
    19741 cttggatcgc ttggtagggt attcgatttc gcctattttg tggaagaagg tcaagaaggg
    19801 cttgtcagca ggtcgcgttc agtccattgc ccttaaactc atcattgacc gtgaaaatga
    19861 aatcaatgcc ttccagccag aagaatactg gacagttgat gctgtcttta aaaagggaac
    19921 caaacaattt catgcttcct tctatggagt agatggtaaa aagatgaaac tgaccagcaa
    19981 taacgaagtc aaggaagtct tgtctcgtct gacgagtaaa gacttttcag tagatcaggt
    20041 ggataagaaa gagcgcaagc gcaatgctcc tttaccctat accacttcat ctatgcagat
    20101 ggatgctgcc aataaaatca atttccgtac tcgaaaaacc atgatggttg cccaacagct
    20161 ctatgaagga attaatatcg gttctggtgt tcaaggtttg attacctata tgcgtaccga
    20221 ttcgactcgt atcagtcctg tagcgcaaaa tgaggcggca agcttcatta cggatcgttt
    20281 tggtagcaag tattctaagc acggtagcaa ggtcaaaaac gcatcaggtg ctcaggatgc
    20341 ccatgaggct attcgtccgt caagtgtctt taatacacca gaaagcatcg ctaagtatct
    20401 ggacaaggat cagcttaagc tatataccct tatctggaat cgttttgtgg ctagccagat
    20461 gacagcggcc gtttttgata ccatggctgt taaattgtct caaaaagggg ttcaatttgc
    20521 tgccaatggt agtcaggtta agtttgatgg ttatcttgcc atttataatg attctgacaa
    20581 gaataagatg ttaccggaca tggttgttgg agatgtggtc aaacaggtca atagcaaacc
    20641 agagcaacat ttcacccaac cgcctgcccg ttattctgaa gcaacactga ttaaaacctt
    20701 agaggaaaat ggggttggac gtccatcaac ctatgcgcca accattgaaa ccattcagaa
    20761 acgttattat gttcgcctgg cagccaaacg ttttgaaccg acagagttgg gagaaattgt
    20821 caataagctc atcgttgaat atttcccaga tatcgtaaac gtgaccttca cagctgaaat
    20881 ggaaggtaaa ctggatgatg tcgaagttgg aaaagagcag tggcgacggg tcattgatgc
    20941 cttttacaaa ccattctcta aagaagttgc caaggctgaa gaagaaatgg aaaaaatcca
    21001 gattaaggat gaaccagctg gatttgactg tgaagtgtgt ggcagtccaa tggtcattaa
    21061 acttggtcgt tttggtaaat tctacgcttg tagcaatttc ccagattgcc gtcataccca
    21121 agcaatcgtg aaagagattg gtgttgagtg tccaagctgt catcagggac aaattattga
    21181 gcgaaaaacc aagcgtaatc gcctcttcta tggttgcaat cgctatccag aatgtgaatt
    21241 tacctcttgg gacaagcctg ttggtcgtga ctgtccaaaa tgtggcaact tcctcatgga
    21301 gaaaaaagtc cgtggtggtg gcaagcaggt tgtttgtagc aaaggcgact acgaggaaga
    21361 aaagattaaa taagaggagg ggcccgaaag taaatttcag gctccttttg gttagaactt
    21421 gacaaaatac atcattttat gcgaaactag aagaagatta ttttaactag gagaagttat
    21481 gcgtcttatc tatctgataa ttggtttttt atcactggcc ttggctattg ttggggttgt
    21541 tttacccttg ttgcctacaa cacctttcct tttgttgtct attgcttgtt tctccagaag
    21601 ttccaagcga ttcgaagact ggctttatca taccaagctc tatcaaacat atgtagctga
    21661 ttttcgcgag actaagtcta ttacgcgtga acgaaagaaa aaaatcatcg tatccattta
    21721 cattttgatg gggatttcta tttattttgc ccctctctta ccagtcaaaa tcggtctggg
    21781 tgctttgacc atctttatca cttattatct cttcaaggtc attccagaca aagaatagtt
    21841 aaaacagtag ttatttacct tgataaaatt gaaagcatat tcataacaat atgatataat
    21901 aaatttgaag taatattcaa ggagaatcaa atgatttacg aattttgtgc tgaaaatgtg
    21961 actttacttg aaaaagcgat gcaggctgga gctcgtcgga ttgaactctg tgataatcta
    22021 gcagttggtg ggacaacacc tagctatgga gtgactaagg cagcggttga actggcagct
    22081 aactacgata caaccatcat gaccatgatt cggccacgtg gtggtgactt tgtctataat
    22141 gacctagaaa ttgctatcat gctagaagac atttgtttga ctgctcaggc tggaagtcaa
    22201 ggggttgtat ttggagcttt aactgctgat aaaaagttgg ataagcctaa tctggaaaag
    22261 ttaattgctg catcaaaagg aatggaaatt gtctttcaca tggcctttga tgaactaagt
    22321 gatgaagatc aaccggaagc tattgactgg ctcagtcaag ccggtgtcac tcgtatccta
    22381 actcgtgctg gtgtgtctgg cgactcctta gaaaaacgtt ttgttcacta tcacagaatt
    22441 ttggagtacg ctaaaggtaa aattgaaatt ctaccaggtg gggggattga ccttgacaac
    22501 cgtcaaacct ttattgacca ggtgggggta acacaattgc atggtactaa ggttgttttt
    22561 taaaaaatag aaaggaactg ctagccttgg gtagcagttt tcacttatgt ttgaaatttt
    22621 taaatcctat caatttaatc aagaaaaggc tcatgattat ggttttatag aaaatagcga
    22681 agtctggaca tatagttgcc agattttgca aggtgacttt gtcatgactg tgtccatcac
    22741 tgctgataat gtgaactttc aagtctttga ccaagagact ggtgacctct atcctcacgt
    22801 tcatatggaa agcatgaggg gaagttttgt cggaaatgtc cgtgaggctt gtctggagat
    22861 tctttaccag attcggaagg cttgttttga tgtgcaagat tttatctgtc atcagactaa
    22921 gcgtatcatg actcaagttc aggaaaagta tggaaaccag ttggagtatc tgtgggaaaa
    22981 atcgcctgat acagctgtat tgcgccatga aggcaatcaa aagtggtatg ccgtcttgat
    23041 gaaaatctct tggaataagc tggaaaaggg cagagaagga caagtggaag cagtcaacct
    23101 caagcatgac caagtagcta atttgctttc acaaaagggg atttatccag ccttccatat
    23161 gagcaagcgc tactggatta gtgtgtccct tgatgatact ttatcagatg aagaagtact
    23221 ggaattgata gaaaaaagtt ggaacttaac ctctaaaaaa tgaaatattt taataatttt
    23281 catgaacttt caattagcta aatattcttt actgaagaaa tttttagaaa atataggatt
    23341 taccacacta gaggaatatg gtgccatctt caaatacctg attgagaatg tcaagacgga
    23401 tcgtcagatc atctgttcgc ctcactgtca tgatgacctc ggaatggcag tggcaaatag
    23461 ccttgctgct gtcaagaatg gtgcaggacg tgttgaaggg actatcaatg gtattaggga
    23521 gcgagctgaa aatgctgctt tggaagaaat tgcagtggct ctcaatattc gccaagatta
    23581 ctaccaagta gaaaccagta ttgtcctaaa tgagaccatc aatacgtcag aaatggtttc
    23641 tcgcttctct ggtattccag ttcctaaaaa caaagccgtc gttggtggca ataccttctc
    23701 ccacgaatct ggtattcacc aagatggagt ccttaaaaat cctctcactt atgagatcat
    23761 cacacctgaa ttggttggtg ttaagattcc gcttggaaaa ttatctggtc gccatgcttt
    23821 tgttgagaaa ctgagagaat tggccctaga ttttacagaa gaggatatca aaccactctt
    23881 tgctaagttc aaggcactgg ccgataagaa gtaagaaatc acagatgcag atcctcgagc
    23941 tttggtagct ggaaccatgg ttgaaaatcc agaaggcttc cactttgatg atttacaact
    24001 tcaaactcat gcagataatg acattgaagc gctcgttagc ctagccaata tggatggtga
    24061 gaaagtcgaa tttaatgcga cagggcaagg ttccgttgaa gcaatcttta acgctatcga
    24121 taagttcttt aaccaatctg ttcgtttggt gtcctacact atcaatgcgg taacagatgg
    24181 aatcgatgcc caggatcggg ttttggtcac tgttgaaaac agagatacag aaaccatctt
    24241 taatgcagca gggcttgatt ttgatgtgtt gaaggcttct gctattgcct atataaacgc
    24301 taataccttt gttcaaaaag agaatgcagg tgagatggga cgcagtgttt cttaccacga
    24361 tatgcctagt gtgtaaagga gaaggctatg gcaaagaaaa tagtagctct agtaggagac
    24421 ggaattggcc cagaaatcat gtaggctggt ttagaagttc tggaggctct agctgaaaaa
    24481 acaggttttg actatgagat tgacagacga ccgttcggag gtgcagatat tgatgcagca
    24541 ggacctccct tacctgatga aacccttaag gcaagtaggg aagcagatgc tatcctacta
    24601 gcagctatcg gtagtcctca gtatgatgga gcagcggttc gccctgaaca aggcctgatg
    24661 gctctccgta aggaactcaa tctttacgct aatattcgtc ctgtaaaaat ctttgacagt
    24721 ctcaagtatt tgtcaccact caaaccggaa cgaatttctg gtgtagactt tgtcgtggtg
    24781 cgtgaattga caggcgagat ttactttgga gatcatattc ttgaagagcg caaagcgcgt
    24841 gatatcaacg actatagcta tgaggaagtg gagcggatta ttcgcaaagc ctttgaaatt
    24901 gcaagaaatc gcagaaaaat cgttactagt atcgataagc aaaatgttct agcgacctca
    24961 aaactctggc ggaaagtagc tgaggaagtc gcacaggatt tcccagatgt aaccttggaa
    25021 caccagctgg tagactcagc tgctatgctt atgattacca atcctgctaa gtttgatgtt
    25081 attgtaacgg agaatctttt tggagatatt ttatctgatg aatcaagcgt cttatctggt
    25141 acacttgagg ttatgccatc agccagtcat tctgaaaatg gaccaagtct ctatgaacct
    25201 attcacggtt cagcacctga tattgcaggt caaggaattg ccaatcctac ttccatgatt
    25261 ttatcagttg ccatgatgtt gagagatagt ttcggacgtt atgaggatgc agagcgtatc
    25321 aaacatgctg ttgagacaag tctggcggca ggaattttaa cgagagatat aggaggtcag
    25381 gcttcaacaa aggaaatgac ggaagctatt attgcaaggt tatgaagtta gacgaaaaaa
    25441 ttactctagt ccttttgatt tggaatgtca tcattttctt gatttatggc attgacaaat
    25501 tcaaggcaag aagaagaact tggcgcatcc aagagaaaat cttactcatt ttagccctta
    25561 cttgtggtgg ttttggggcc tggttagctg gaatcacttt tcaccacaag actcgaaaat
    25621 ggtactttaa aacagtttgg tttcttggga tggtgaccac actagtagcc ttatatttta
    25681 tttggaggta atggatggca gggtcttcga gggaacacgc tgcttgggtt ctagcggact
    25741 atggttttaa ggtcgtgatt gcaggatctt tcggtgacat tcattacaat aatgaactca
    25801 ataatggcct gttgccaatc gttcagccta gagaggttag agagaaacta gcccagctaa
    25861 aaccaaccga ccaggtaact gtggacttgg aacaacaaaa aatcatctca ccagttgaag
    25921 aattcacctt cgagatagat agcgagtgga aacataaact cctaaatagt ttggatgata
    25981 tcggtattac cttgcagtat gaagagttga ttgctgctta tgaaaaacaa cgaccagcct
    26041 actggcagga ttagaaaaaa tagaaaagga aatatagtaa actgaaataa gatgtaaaca
    26101 aatgaattag agcttaacat ccatttccag caatttttta gaaactacag tggactattc
    26161 tggattcaac acattataaa attatgacaa aacacattca caagaaggct acgacatttt
    26221 aaaaggtgag ggcggatgta tcgtttgccc tactaaagtt ggttacatta tcatgaccag
    26281 caacaaggca ggacttgagc gtaagttcgc agccaaagaa cgtaagcgta acaaaccagg
    26341 tgttgttctc tgcggtagca tggatgaact tcgcgcttta gcgcaactca acccagaaat
    26401 tgaagcattc tactaaaaac attggatgaa gatattcttc ttggttgtat ccttccttgg
    26461 aaaccagaag cctttgaaaa actcaaagca tacgggaatg gccgtgaaga acttatgact
    26521 gatgtacgtg gtactagctg ttttgttatc aagtttggaa aagcaggtga acaattggct
    26581 gccaagcttt gggaagaagg taaaatggtc tacgcctcat atgcttcaat gacaaaacga
    26641 ttgaaactcg ctatgagagc aaggtgtaat ggtgtctatg gtcgataagg acggcaaact
    26701 catcccagaa caaggaggag cacgttcaac ttcaccagca ccagttgtga tccgtaaagg
    26761 gcttgacatt gataaaatca tgatgcacct gtcagatact tttaactcat gggactaccg
    26821 tcaggttgag tattattaag ataaaaaaag aagtctagtg ttatgagata ttaaagctcc
    26881 taacactgga ctttttgttt agaatttctt ttctttttct ataggatatg gtattctatg
    26941 tagaaaatat atgttaataa gtaatgccaa tatttaaaca tcattagtaa aaggagttag
    27001 attgatgaat aaaagaaaag ttagtttaga agatttttat aaatggtata gtctaaataa
    27061 agaagagtta ttaaataagg caactgttgg tgaaaagttt aatgataaat taaaagaaga
    27121 gtttctccag gaatggcctt tggataggat tttaacaatg tcaatcgatg aatatgtaat
    27181 aggaaaggga cagcaaaata agtctttatg ctacgctctt gagaagggaa aatacaaaaa
    27241 tctatttctt ggaatttctg gtggctcagc ttcaaaattt ggtatttatt ggaataaaaa
    27301 aacaaacaaa tataaagatc aagctaataa tgagatttca gagttggatc agcgattttc
    27361 aaaattaaaa tcagatttgt atgaaattat caaagaaggt attcgtttta actttgaaaa
    27421 tcctattttt gatatgaaaa gatcaacaaa tgaatttatt ggtcgttctg ctatggtgac
    27481 aaaattactt tgtatctata ctgagggaga tcctttcttt ggtgtaaata ttaatagtca
    27541 gaaagaattt tggaaccact ttgtttctca gacaaatcaa ggtggacctt atctgcaaaa
    27601 tcataaaata attgaactag tgtccaaaac ttatcctgag ttggagccat cgaaattagg
    27661 aactatgctt tttgagtatt ctaagctttt tatggaaaat aaggaagaca atagtacaat
    27721 ggattcatca aacaattttc gtcatcaatt aactcaatct ctattaaagt ctccaaacct
    27781 catcctccgt ggtgctcctg gcacaggaaa gacttatctt gctaaagaaa ttgccaaaga
    27841 attaacggat ggcaacgaag accaaatcgg atttgtacaa tttcacccat cctatgatta
    27901 tacggatttt gtagaaggtt taagaccagt atcaaatggg gatggagcta ttgagtttag
    27961 gctacaggac ggtattttta aagatttttg tcagaaagca aaagaaaccc aattgattgg
    28021 aggacaagat aattttgatg aggcttggga ttcttactta gaatatataa atgttgctga
    28081 agaaaaagaa tatataacaa aaacatctta cttatctatt aatagtagac aaaatttgtc
    28141 agtaaattat gatagtggtg tttcaggatg gtcactacct agcaaatatg tttacgagtt
    28201 gtataaagat aaaaattata ataagcaaga atactacaaa agtggtggaa aaactgtcct
    28261 agaaacattg agaaagagat ttggtttgaa agactatgtt tccccaacag aaattgatac
    28321 tgataagaaa ttcgtcttca tcatcgatga aatcaatcgt ggggagattt ctaagatttt
    28381 tggcgaactc tttttctcta tcgaccctgg ctatcgtggt gaaaaaggaa gtgtttctac
    28441 ccaatatgca aatctacacg aaactgatga aaagttctat atccccgaaa atgtttacat
    28501 catcggaact atgaatgata ttgatcgttc agtggatacc tttgattttg ctatgcgtcg
    28561 tcgttttcgt tttgttgaag ttactgccga gggtcaagtt ggcatgttgg ataaagagct
    28621 gaatatccat gcagaagaag caaaaattcg tctaagaaac ttgaacgctg ctatcgaaaa
    28681 tgttcaggaa ttaaacagtc attatcatat tggaccaagt tattttctta agttgaagga
    28741 tgtagatttt gactatgaat tactctggtc tgattatatt aagcctctcc tagaagacta
    28801 cttgcgaggt tcttatgatg aggttgaaac tttggaaact ttgaaaaaag catttgaact
    28861 gacaaataat gagcaaaaag atcaggcagt agctgatgac aatgaaggtg atgaaaacga
    28921 tgatgcggat tactgataat caacacaaga ttattaaaga aaaatttgtt gaagaatatc
    28981 ctaaactaag caatcttctt ttagacagaa ccttggaaag tctatcccaa gatgaacgta
    29041 ttttcatttt tccaaatgat ttgactcata ctcctgattt ggataaggac caaaagattt
    29101 ttgaaacagt caatcagaaa atcaagacag ggaacgtgat tggttttctt ggatatggtc
    29161 aggaaagatt aacgatttcc tcacgatttt ctgatgagag taatgaccat tttttgcatt
    29221 atctcttaaa caaggttctt tatatcaatc tcactagttt agatgttgct ttgtctcgtg
    29281 aagagaggct ttatcaactt ttgatgtatc tcttccccaa gtatctacaa gctgctattc
    29341 gaaaaggtct ttataaggaa tatcatcgat tttttcataa cgacagtaat gttaagggag
    29401 tgattgatgt aagaagccat ctcaagaaaa atcttccttt cacgggaaat attgcctacg
    29461 caacgagaga gttcacctat gataatcccc tcatgcagtt ggtccgtcac actattgaat
    29521 acattaagaa tcagaaaagc attggtcaag gggtactaga taatctctca actagtcgtg
    29581 aaaacgtatc tgaaatcgtg cgtgtaacgc cctcttataa actagctgat cgtgctaaga
    29641 ttactcggag aaatcaatct aaacctatac gtcatgcata ctttcacgag tacagaaact
    29701 tacaagaact ttgtctgatg atcctaaacc aagaaaagca cggtttaggg tatcaagatc
    29761 aaaaaatcta tggtattctc tttgatgttg cctggctttg ggaagagtat gtttacacct
    29821 tgttgccaaa aggttttgta catcccagaa ataaggataa gacggatgga atttcagtat
    29881 tttctgttgg gaaacgaaaa gtatatccag atttttatga cagagaacga aagattgttc
    29941 tagatgcaaa atataaaaaa ctggaattga ctgaaaaagg aatcaaccgt gaggacttat
    30001 tccagctgat ttcctattct tatattttaa aagctgagaa ggctggactg atttttccta
    30061 gtatggagca gtcagtaaat agtgaaatag gaaaagtagc tggctatgga gctcaattga
    30121 agaagtggtc tattcgaatc cctcagaatg cctcatccta tagtgcattt tgtaaaatga
    30181 tggaaaattc agaagaaaat tttaaagcga ttattgatga agaagtgggg agaaaataat
    30241 ttcccctctt tcttttattt ttaacacaag taaaacccga acgatatatt ttaattcata
    30301 aaaaaaattg acaaaaactg tactttggtt tataataaat taaggaaacg tcggaaaagg
    30361 cgtagtgatg cgagagcata ggtaggtcat tacaaaagaa acgagacatc gatatgttaa
    30421 atgaatttcc aatttttgat tacgaagata ttcaattgat tccaaataaa tgtgtcatta
    30481 aaagccgtgc agaagcggat acaagtgtca ctttaggaaa tcacaccttt aaactacctg
    30541 ttgtgccagc gaatatgcag acgattttgg atgaaaatgt agcagagcaa ctggctaaag
    30601 gtggttactt ctacattatg caccgttttg atgaggcagg acgcattcct tttattaaac
    30661 gaatgcacaa tcaagggctc attgcttcca tatctgtcgg tgttaaggac tatgagtatg
    30721 atttcgttag acagctcaag actgatgctc ctgagtatat cactattgat attgctcatg
    30781 gtcatgcgga tagcgtgatt tctatgattc aacacatcaa gaaagaattg ccagatacct
    30841 ttgtcattgc tggaaatgtg ggaacaccag aagctgtgcg tgaattggaa aatgctggtg
    30901 cggatgctac taaggtcgga atcggtcctg gtaaggtttg tatcaccaag gttaagactg
    30961 gttttggtac aggtggttgg cagttggctg ctctacgctg gtgtgtcaag gctgcgcgta
    31021 aaccgattat cgctgatgga ggaattcgta ctcacggtga tattgccaag tctatccgct
    31081 tcggtgctag catgatcatg attggttccc tctttgcagg acatattgaa agtccaggga
    31141 aaacgattga agtcgatggt gaacagttca aagaatatta tggttcagcc tcacaatatc
    31201 aaaaaggtgc ttacaaaaat gtggaaggca aacgtatctt gcttcctgct aaaggtcatc
    31261 tgcaagacac tttaactgag atggaacaag accttcaaag tgctatttct tatgcaggtg
    31321 gacgtcaggt tgctgacctt aaacacgttg attatgtgat cgtgaaaaac tccatctgga
    31381 atggggatgc ttcccactaa gacggggtct ttgactaaaa aacttgttat tagagcaaat
    31441 ttctgttata ataaaacaag tttccaccct tagtgtaatg gatatcacgt aagattccgg
    31501 ttcttgagat gggggttcga ttccctcagg gtggatgtaa acattctaaa aagcctttaa
    31561 tagggctttt ttctttacac tgctctaaat ttaccgctaa aactgagaat ctctgtctaa
    31621 agaatttcca aatgctttcc agtcccgttt tagagtgact cagggggctt tttttgatat
    31681 aataaaaagg actgttatca gttagaaaga ggttggtatg aaagaattac aaactgtact
    31741 aaagaaccat tttgcaatcg aatttacaga caaaaagtta ctggaaactg cctttactca
    31801 tacgagttat gccaatgagc accgcctctt aaaaatttca cataatgaac gcttggaatt
    31861 tttaggagac gctgttctac agttattgat ttcagaatat ctgtataaaa aatatcctaa
    31921 aaagcctgaa ggtgacctat caaaactccg tgctatgatt gtccgtgagg agagtttagc
    31981 tggttttgcg cgtgattgcc agtttgacca gtttatcaag ttgggtaaag gggaagaaaa
    32041 atctggtggt cgcaatcgtg acaccattct tggtgatgcc tttgaagcct ttcttggtgc
    32101 ccttcttttg gataaggatg tggccaaggt caaggaattt atctatcaag tcatgattcc
    32161 taaggttgaa gcaggcgaat ttgagatgat tacagactat aaaacccatc tccaagagtt
    32221 acttcaggtc aatggtgatg tggctattcg ttatcaggtg atttctgaaa cagggcctgc
    32281 tcacgataag gtctttgatg tagaagttct tgttgaaggt aagagtatcg gtcaaggcca
    32341 aggtcgttct aagaaattag cagagcagga agctgccaaa aatgccgttg agaaggggct
    32401 ggattcatgt atttaaagga aatcgaaatt caggggttca agtcttttgc tgataagacc
    32461 aaggtcgttt ttgaccaagg tgtgacggca gttgttggac caaatggatc tggaaagtcc
    32521 aatattacag aaagtctgcg ttgggctttg ggggagtcta gtgtcaagag tctccgtggg
    32581 ggcaagatgc cggatgtcat ctttgctgga acagaaagtc gcaaaccgct caattatgct
    32641 tctgtagttg tgactctgga taatcatgac ggatttatca aggatgcagg tcaagaaatc
    32701 agggtggaac gccatatcta tcgtagtgga gatagcgaat acaagattga cggcaagaaa
    32761 gtccgtctgc gtgatattca tgacctcttc ttggatactg gattgggacg agattccttc
    32821 tctattattt cccaagggaa ggttgaggag atttttaatt ccaagcctga ggaacgacga
    32881 gctatttttg aagaagctgc tggagtttta aaatacaaga ctcgcagaaa agaaaccgag
    32941 agtaaactgc agcaaactca ggataatctg gaccgcttag aggacattat ctacgagttg
    33001 gataatcaaa tcaagcctct tgagaagcaa gctgagaatg cccgtaagtt tttagacttg
    33061 gaaggacaac gtaaggctat ttatttagac gttctggttg ctcaaatcaa ggaaaataag
    33121 gctgaactag agtcgacaga agaagagttg gctcaggttc aagaactctt gatgagttat
    33181 taccaaaagc gtgaaaaatt agaagaagaa aatcaaactc ttaaaaagca acgccaagat
    33241 ttacaggctg aaatggccaa agaccaaggc agtttgatgg atttgaccag tctgattagt
    33301 gacttagaga gaaaattagc cctatcgaaa ctggaatctg agcaagtagc cctcaatcaa
    33361 caggaagcac aagcccgttt ggctactttg gaggataaga gaagttcact cagccaagaa
    33421 aaatctgata aagaaagctc tttagccctc ttggaagaaa atctagtcca aaataatcaa
    33481 aaactcaatc gattagaggc tgaattgcta gctttttcag atgatcctga tcagatgatt
    33541 gagctcttac gtgaacgctt tgtggccctt ttacaagaag aagcagatgt ctcaaaccaa
    33601 ctgacccgta ttgagaatga gttggaaaat agtcatcagc tttctcaaaa acaagcagat
    33661 caactagaaa agctgaaaga acagttggct acagctaaag agaaggctgg tcagcaaaag
    33721 gctgagcttg aaactgccaa ggagcaggtt cagaaattat tggccgacta tcaagtctgt
    33781 gctaaggagc aagaggagca gaaaacttcc tatcaagctc aacaaagtca actctttgac
    33841 cgtctggata gtctcaaaaa caagcaggct agagcgcaaa gtttggaaaa tatcctgaga
    33901 aatcatagta acttttatgc aggtgttaag agtgttctcc aagagaaaga tcgtctaggt
    33961 gggattattg gagcagttag tgagcacctg acctttgata tgcattatca aactgcccta
    34021 gaaattgcgc ttggagcgag tagtcagcac attattgtag aagatgagaa tgcggcaacc
    34081 aaagccattg atttactcaa acgaaacaga tctggtcgtg caaccttcct tcctttgacc
    34141 actatcaagg ctcgtacgat ttctagtcag aaccaagata ctattgctgc aagtccagga
    34201 tttctgggca tggcagatga gctggttact ttcgatacta gactggaagc cattttcaag
    34261 aatttgctag ctacaacggc catttttgat accgtggagc atgcgcgtgc agcagcgcgc
    34321 caggttcgtt atcaggttcg tatggtgaca ttggacggaa cagagctgcg cacaggtggt
    34381 tcttatgcag gtggagctaa tcgccagaat aacagtattt tcatcaagcc agaactggag
    34441 caattacaaa aagaaattgc tgaagaagaa gcaagtttgc gttcagatga agtgagtttg
    34501 aagaacttgc aagatgagct agctagattg acagaaagat tagaagccat caaatctcag
    34561 ggagagcagg ctcgtattca ggagcaaggc ttgtccctcg cctatcagca gaccagtcag
    34621 caagttgaag agctagaaac tctttggaaa ctccaagaag aggaattaaa tcgtctttct
    34681 gagggagaat ggcaagcgga taaggaaaaa tgccaagagc gccttactac aatcactagt
    34741 gaaaagaata atctggaagc tgagattgaa gagattaagt ctaataaaaa cgccatccaa
    34801 gaacgttatc aaaacttgca ggaagagcta gctcaagctc gtctacttaa gacagaactg
    34861 caagggcaaa aacgttatga agttgctgat attgaacgct taggtaagga actagataat
    34921 cttgatattg agcaagaaga aattcagcgc cttcttcaag aaaaggttga caatcttgag
    34981 aaagttgata cggatttgct cagtcaacag gctgatgaag ccaaaactca aaaaacaaat
    35041 ctccaacaag gtttgattcg caagcagttt gagttggatg atattgaggg tcaactggat
    35101 gatattgcta gtcatttgga tcaggctcgc cagcagaatg aagagtggat tcgcaagcaa
    35161 actcgtgctg aagccaagaa agaaaaggtt agcgagcgct tgcgccatct acaaaatcaa
    35221 ttaacagacc agtaccagat tagctatact gaagcactag aaaaagcgca tgagttggaa
    35281 aatctcaatc tggcagagca agaggtgcag gatttagaga aggctattcg ctcactggga
    35341 cctgtcaatt tagacgctat tgaacagtac gaagaagttc acaaccgtct ggatttccta
    35401 aatagccaac gtgatgatat tttgtctgcg aaaaacctgc ttcttgaaac catcacagag
    35461 atgaatgatg aggtcaagga acgctttaaa tcaacctttg aggccattcg tgagtccttt
    35521 aaagtgacct tcaagcagat gtttggtgga ggtcaggcag acttgatatt gactgagggc
    35581 gaccttttaa cagctggtgt ggagatttct gttcaacctc caggtaagaa aatccagtcg
    35641 cttaacctca tgagtggtgg tgaaaaagct ctatcggctc ttgccttgct tttctccatt
    35701 attcgtgtca agaccattcc ttttgtcatc ttggatgagg tagaggctgc gctagacgaa
    35761 gccaatgtta aacgtttcgg ggattatctc aaccgctttg ataaggacag tcaatttatt
    35821 gtcgtaaccc accgtaaggg gaccatggca gcggccgatt ctatctatgg agtgaccatg
    35881 caagaatcgg gtgtttcaaa gattgtttca gttaagttaa aagatttaga aagtattgaa
    35941 ggatgacaat taaactagta gcaacggata tggacggaac cttcctagat gggaatggac
    36001 gctttgatat ggatcgtctc aagtctctct tggcttccta caaggaaaaa gggatttact
    36061 ttgcggtggc ttcgggtcgg ggatttctgt ctctggaaaa attatttgct ggtgttcgtg
    36121 atgacattat tttcatcgcg gaaaatggca gtttggtaga gtatcaaggt caggacttgt
    36181 atgaagcgac tatgtctcgt gacttttatc tggcaacttt tgaaaagctg aaaacgtcac
    36241 cttatgtaga tatcaataaa ctgctcttga cgggtaagaa gggttcatat gttctagata
    36301 cggttgatga gacctatttg aaagtgagtc agcattataa tgaaaatatc caaaaagtag
    36361 cgagtttgga agatatcaca gatgacattt tcaaatttac aaccaacttc acagaagaaa
    36421 cgctggaagc tggtgaagct tgggtcaatg ataatgtccc tggtgtcaag gctatgacaa
    36481 ctggctttga atctattgat attgttctgg actatgtcga taagggtgta gctattgttg
    36541 aattagctaa aaaacttggt atcacaatgg atcaggtcat ggcttttgga gacaatctta
    36601 atgacttaca tatgatgcag gttgtgggac atcctgtagc tcctgaaaat gcacgacctg
    36661 aaattttaga attagcaaag actgtgattg gtcaccataa ggaacggtcg gttatagctt
    36721 atatggaggg cttataatgg cagatataaa attgattgca ttggacttgg acgggacctt
    36781 gctgactact gataaaaggc tgacggatcg taccaaggaa accttgaaag ctgcgcgtga
    36841 tcgtggtatc aaggtcgtat tgacaactgg tcgtccctta aaagccatgg atttctttct
    36901 ccatgagtta gggactgacg gtcaggaaga tgagtatacc attactttta atggtggatt
    36961 agttcagaaa aatacaggag aaatccttga taaaacagtc ttttcatatg atgatgtggc
    37021 acgtttgtat gaagaaacag agaaattatc actgcctctc gatgccatct cagaaggaac
    37081 agtttatcaa atccaatcgg accaagaaag tctttatgcc aaattcaatc cagctttgac
    37141 ctttgttcca gtggactttg aagacttatc tagtcaaatg acctacaaca aatgcgtgac
    37201 tgcctttgct caagaaccct tggatgcagc cattcagaag atttctccag aattgtttga
    37261 ccaatatgaa atctttaaat cacgtgaaat gttgctagaa tggtcaccaa agaatgttca
    37321 taaagcaaca ggtttggcaa aactaatcag ccatcttgga atcgaccaaa gtcaagtgat
    37381 ggcttgtggt gacgaggcca atgacctctc tatgattgaa tgggcaggtc ttggtgttgc
    37441 tatgcaaaac gctgttcctg aagtaaaggc agccgcaaat gtagtgacgc cgatgaccaa
    37501 cgatgaggaa gctgtcgcct gggctatcga agaatatgtg ctaaaggaga actaagatat
    37561 gggattgttt gaccgtctat tcggaaaaaa agaagaacct aaaatcgaag aagttgtaaa
    37621 agaagctctg gaaaatcttg atttgtctga agatgttgat cctaccttca cagaagttga
    37681 ggaagtttct caggaagaag cagaggttga aattgttgaa caagctgtgt tccaagaaga
    37741 ggaaatccaa gacacagttg aagaaagtct ggatttagag ccagttatag aagtttctca
    37801 aaaagaagtc aaagaatttc cacactcaga agaagggaat actgagtttc tagagactgt
    37861 agaagaaaat aattctgaaa ttcttgaagc agaaaggcct caaacagaag aaactgttca
    37921 ggaaaaatat gaccgcagtc ttaagaaaac tcgcacaggt ttcggtgccc gcttgaatgc
    37981 cttctttgct aacttccgct ctgttgacga agaatttttc gaggaactgg aagaactgtt
    38041 gattatgagt gatgttggtg tccaagtcgc ttctaactta acggaggaac tacgttacga
    38101 agccaagctt gaaaatgcca agaaacctga tgcacttcgt cgtgtcatca ttgagaaatt
    38161 ggttgagctt tatgaaaagg atggtagcta cgatgaaagc atccacttcc aagataactt
    38221 gacagttatg ctctttgttg gtgtgaatgg tgttgggaaa acaacttcta tcggaaaact
    38281 agctcaccgc tacaaacgag ctggtaagaa ggtcatgctg gttgcagcag ataccttccg
    38341 tgcgggtgca gtagctcagc tagctgaatg gggccgacga gtagatgttc cagtagtaac
    38401 tggacctgaa aaagctgatc cagccagcgt ggtctttgat ggtatggaac gtgccgtggc
    38461 tgaaggtatc gatattctca tgattgatac tgctggtcgt ctgcaaaata aggataacct
    38521 tatggctgag ttggaaaaga ttggtcgtat tatcaaacgt gttgtgccag aagcaccaca
    38581 tgaaaccttc ttggcacttg atgcatcaac aggtcaaaat gccctagtac aggccaaaga
    38641 attttcgaaa atcacacctt taacgggaat tgttttgact aagattgatg gaactgctcg
    38701 aggaggtgtg gttctagcca ttcgtgaaga actcaatatt cctgtaaaat tgattggttt
    38761 tggtgaaaaa atcgatgata ttggagagtt taactcagaa aacttcatga aaggtctctt
    38821 ggaaggttta atctaatcag aagcaaaaat cctgcaaggc ataaacttgc aggaaatttt
    38881 tttattctaa gcgaccatct tgacgatagg cgatatctgg ttgccaagtc catttggcac
    38941 cgaatttttc aagtaggtca aagctggctt gaggtcccat gcttccagct ttatagtcat
    39001 gaagtggggc accattttca tcccagagct cttcaatacg gtcaatcaac ttccatgatg
    39061 caccaacttc atcccagtgg ctaaagttag ttgagttgtt atttaggaca tcataaatca
    39121 atttctcgta tggttctgga gaagcaccag ttgcagtcgc atctgtacgg taatcaagtg
    39181 agttaggagc caagttaaat tcttctccta cttgcttccc atttaggcta agagagaagc
    39241 cttctgttgg ttgaatatag atggtcaaaa tatttggagc aagtggttct ccaaagatag
    39301 aatccatttg tttaaagacg atgttgacat gagttccttt ttcagtcagt cgtttacctg
    39361 tacggaaaaa gaaaggaaca ctacggaatc gatcgctgtc tacaaagaag gcaccagatg
    39421 taaaggtttc agttgttgat tctggattca catttggctc gctacgataa gagatgtatt
    39481 tcatgccatc aatcttacca gagcggtatt gcccacggat aaagtgttct ttgagttctt
    39541 catcagttgg atgatagagg tttttaaaga ccttaatctt ttcagcacga atctcgtctt
    39601 ttgtgaagct tgctggtttg tccatggcga ggagcgaaag aagttgtaga gtgtggtttt
    39661 ggaccatgtc acggagggca ccggattggt catagtagcc accacgttct tctacaccca
    39721 agcgctccgc aaaggtaatt tgaacattgt cgataaaatc cttgttccaa acgttttcaa
    39781 aaatcaagtt tgcaaagcga actgcaaaga tgctttggat catttcctta ccaagataat
    39841 ggtcgatacg gaaaatttgt tcttcgtcaa atgttgctag gagttcgtca ttcaacttgc
    39901 ttgcagttgc gtaatctgta ccaaatggtt tttcaacgat caagcgctca aaacctttgc
    39961 catcgacaat gttttcagat ttgaggtgtt tggcaatggt tccaaagaac tgaggtgcca
    40021 tagacaagaa gaagagctta ttgtgttcag cttggtattt ttcattaagc tcagcttgta
    40081 attgacgcaa agcaatataa tgttccgaat cattgacatc atggctttga tagtagaagt
    40141 ggctagcaaa ttcttgggct tgctcggtac tatctgccaa atcaaggatg gactcgacaa
    40201 ctacagattc aaaatattcc ttactccaag gtctacgggc agttccaata acggcaaagt
    40261 gcttggaaag attgccggat ttatatagtc taaaaaggga agggtagagc ttgcgtttag
    40321 ccaggtctcc actcgcaccg aaaattgtaa caataacctt agatgacatc tagctaccta
    40381 atttctattt ttattcctag tcttgctagg tatgaggaaa cctcatttca taaacttaat
    40441 tctaacataa ttttccgaaa aatcaaaaat tccaatacga agtagaaaaa ctgcaaggaa
    40501 atccttacag tttgagttta gacgtttaag accttatcta agaactcttt cagacgaggg
    40561 tgttgtgggt tatcaaagat ttggtcaggt gttccgtctt caaggaactc gccatctgca
    40621 gtaaagataa cgcggttggc aacctgacga gcaaatccca tctcatgggt tacgataatc
    40681 atggtcatac cttgctcagc caattccttc ataacgttaa gtacgtctcc aaccatctca
    40741 gggtcaaggg cagaagttgg ttcatcgaag agcatgatgt ctggattcat tgctaggcca
    40801 cgagcgatgg ccacacgttg tttttgacca cctgataggc tatctggatt ggcattagct
    40861 ttatctgcta gtccaacctt ttcaagcaac tccattccca attcctcagc ttcttcctta
    40921 gtcatcaact tgtgctcaat aggagcaaag gtgatgttgt ccaatacaga catatgaggg
    40981 aagaggttga agtgttggaa taccatgccg atattttcac ggacgtggtc aacattggtt
    41041 gttttttcag ttaaatcata gccgttcaca gtgatgtgac cgctagtgac ttcttctaaa
    41101 agattgaggc tacggaggaa agttgactta ccagaacctg aaggaccgat gatacaaaca
    41161 acatctcctt catagaactt agtcgtaatt ccttttagga cttcattttt tccatagtgc
    41221 ttgtgtaaat catttacatc aatttttaat tttgccatta acgaatcctc ttttctaagc
    41281 gtttcgctag tctagtcaaa agtgtgataa ttacaagata gaagatagca aggattgcat
    41341 acatcttgaa actttggtag ttacgagcaa tgataatctt accagtttgg aagagttcaa
    41401 ccaaaccgat agcagatacg atagttgtat ctttaagagc gataacgaat tggttgacaa
    41461 agtttggcaa catcaattta gttgcttgtg gcaagataat cttacgcatg gtttttccat
    41521 aagagatacc caagcttcgg ctggcttcca tttggccaac tggaacggcc tgaataccac
    41581 cacgaacgat ttcagcgata taagcagccg cattgagtga gagggcaatg gttccagcta
    41641 caaagtcgtt aattgggctt tgttggcctg tgatagactc gatgaagttt ggaattcccc
    41701 agaagatgaa ggctgcaaga atcatcaatg gaataccacg aataacgtca acgaaaatct
    41761 cagagatgac gcgaagagat ttgtatgggc taacgctaaa cataccgaag ataattccga
    41821 tgacaatggc aatagcaaat gagataagag ctagagcaag agtgatacca agaccgctaa
    41881 ggagttgttt gtagttgttt tgaagcaagc cccagagcgt tgtttcgtca acagtacttg
    41941 ttgaagcagt tgaagattcg ctagctaggt atttgtcaag aatcttttgg aattcaccgt
    42001 ttgctttaag gtttgcaagt ccgttgttga acatttcaat cagttctgga tttgctcctt
    42061 ttttaacggc aaaggctgtt tcaccgattg gagttccaga gattggagtt ttcaattttt
    42121 gaccttggct gatagaatat ttgagaacag gttcatcatc cataacggca tcaatggcac
    42181 cagtgtttaa actgtcatac attgaagaac catcagcaaa ggttttgatt ttgtaaccgt
    42241 atttgctttg attttctgtt aggaaggttt gagaagcagt tccgttttta acaccgactg
    42301 tctttccttt tagatcttca taagaagcaa tattgcttga ttctttgaca ccaagaatgg
    42361 tattagcagt gtagtatgat tctgagaagt caaaagttgc cttacgagca tctgtgacag
    42421 acatcccagc aatgatacca tcggcttgac cagcttggac agcactgata gcagcatcaa
    42481 aaccagggtt ggtgatttca atttcaaaac cttggtcttt agcgattgcc ttaatcaatt
    42541 ccatatcaat accagtgtat tggttgcttg aattttggaa aacaaaaggt gcaaaagaag
    42601 aatcgctggc aatgatatat ttagccttaa caggaatagc ttttaatcct gcgagagtag
    42661 ttgtagttgg cactgctgaa gatgatgaag cagtccattt cttgataatt ttatcaagac
    42721 taccatcttt tttcatttca gccaaggctt ggttaaattc agtaaccagg tgctcgtatt
    42781 tacttccttt tttcacaccg aaagcaaaac ttcctacagc ttcaccatcc atttcaatat
    42841 ggagatcttg accttggtta atggcatatt cgataacagg tttgtcatcc atcatggcat
    42901 cgatggcacc agcactcaag ctgttgttca ttaaatcacc agtgtcaaat gttttaatag
    42961 taaagccgta tttatctttg attgtttcaa ggaaacgttg agcggcagtt ccgtttttaa
    43021 caccaacggt tttgccagtt aattggtcgt acttgctaat tttgtgtgac tttgtagtag
    43081 caatgacaac ttttgtatca tagtaagtat cagacatggt gaagactttt tcacgttctt
    43141 tagtctttgt catccctgcc atgatagcgt cggcttgccc agcttgaacc gcattgactg
    43201 ctgcgtcaaa tccaggatag gacatctgaa tgttccagcc tttaatctca gcgactttgt
    43261 taataatgtc aacatcaatt cctttataag tttgatctga atctttaaac tcaaaaggtg
    43321 cataggcggt atcagaaaca atcttaatcg tttctgcttt ggcaatacct aatgagaaaa
    43381 ttgggaataa aattagcaaa aatgctagaa attttttctt catttttagt ctccttttcc
    43441 gaatattctc tcattatatc agaaaaagta aaaaaaggca aattgttata tttttcgtgt
    43501 cagaaaatat aacattgatt tttccttttc tttcttgaat tgctattaaa aagtgctata
    43561 ataatagtat atatagaaga aaagaggacg gggatatgaa gaacaaaaga atatttaaag
    43621 acttccaagc ttcaaaaatg agtttaaaca tttacacaag ccccttgtta gcctttgttt
    43681 ttgtcttcat aggagagttt gtggctttta ctttgtatgg tattggcttg ttagctctca
    43741 tcggacttgc tagaaatttt ggagaggctg gtcaaaatct tgcaagctac ttgcagacct
    43801 tgcaacagag cttgacggat aaaacaagtg actttcgttt aattttagga ttactggcct
    43861 ttggttttat tcttaacact gtgttcagat ggacaagaaa agttgagaaa agacctattc
    43921 gaaccttggg attttataga gagaatttcc tcagcaatct tctgaaagga tttagtctag
    43981 gcttggcact ttttcttctg accttgttag gtttagtggt cttaggtcaa tatcgtttgg
    44041 aatccattca cttgaatcct tattctcttg cctttgtcgt ctttactatc ccattttgga
    44101 ttttacaggg gacagcagaa gaagtggtgg cccgtgcttg gctacttcct caattggcct
    44161 caagaaccaa tctaaaacta gctattctta tatctagcct gttctttacc ctgcttcata
    44221 tgggcaattc tagcctcacc cctctatctc tagtaaatct ctttttattc ggagttgcca
    44281 tggctcttta tcttctcaaa actgatacag tttggggtgt tgcaggtatt catggtgctt
    44341 ggaattttgc tcagggtaat ctctttggga ttttagttag tggtcaaccg tcaggaacgt
    44401 ctctgatgac ctttttacca caaggcaatc aagattggct atcaggtggt tcttttggca
    44461 tagaaggttc cattatgaca agtctggtat tactactgct gattgtctat cttgctaata
    44521 aattaaagaa agaaaatgaa aggatgtgac ttcggtccgt ccttttcttc gtgaaaatac
    44581 tataagtatg ctaaaatagg aatagcacat ggagagagga ttcttatgat caatcacatt
    44641 acagataatc aatttaaact agtatcaaaa tatcaaccat caggagatca accccaagct
    44701 atcgagcagt tggtggataa cattgagggg ggagaaaaag ctcagattct gatgggggcg
    44761 actggaacag ggaagaccta tactatgagt caggtcattt ctaaagtcaa taaaccaact
    44821 ctggttattg cccacaataa aactctggct ggtcagctct atggggagtt taaggaattt
    44881 ttccctgaaa atgcagttga gtatttcgta tcctactatg attattacca gccagaggcc
    44941 tatgtccctt ctagcgatac ctatattgag aaggatagtt ctgtcaatga cgagattgac
    45001 aagcttcgcc actcagctac ctcagccctt ttggagcgta atgatgttat tgtcgtggcc
    45061 tcagtctctt gtatctatgg tttgggttcg cccaaggaat acgctgatag tgtcgttagt
    45121 ctccgtcctg gtctagagat ttctcgtgat aaactcttga atgacttggt cgatattcag
    45181 tttgaacgta atgatattga tttccaacgc ggaagatttc gtgttcgtgg ggatgtggta
    45241 gagattttcc cagcttcccg agatgaacat gcctttcgag tagaattttt tggagacgaa
    45301 attgaccgta ttcgtgaagt tgaggctctg acaggtcagg tgttgggaga agtggatcat
    45361 ttagcgattt tcccagcgac acactttgtg accaatgacg accacatgga agttgccatt
    45421 gcaaagattc aggccgagtt ggaggagcag ttagctgtct ttgaaaagga aggtaaactg
    45481 cttgaagccc agcgtttgaa acagcggaca gagtatgata tcgaaatgtt gcgtgagatg
    45541 ggctatacca atggggttga aaattattct cgccacatgg atggacggag cgaaggagag
    45601 cctccttata cgcttctcga cttcttccca gatgatttct tgattatgat tgacgagagt
    45661 catatgacca tagggcaaat caagggcatg tacaatggag accgttcgcg taaagaaatg
    45721 ctggttaatt atggtttccg tttgccgtct gctttggaca atcgtcctct ccgtcgggag
    45781 gagtttgaga gtcacgttca tcagattgtt tacgtttcag cgacacctgg tgactatgaa
    45841 aatgaacaga ccgagacagt gattgagcaa atcattcgtc caacgggact cttggatcca
    45901 gaggtggaag tccgtccgac tatgggacag attgatgacc tcttgggtga aatcaatgcc
    45961 cgcgttgaaa aaaatgagcg tacctttatc acaactttga ccaagaaaat ggcagaggac
    46021 ttgaccgact acttcaagga aatgggtatc aaggtcaagt acatgcactc ggatatcaag
    46081 accttggaac ggacggagat tatccgtgac ctgcgcttgg gtgtctttga tgtcttggtc
    46141 ggaattaacc tgctccgtga aggaattgac gttcctgaag tgagcctcgt agctattctc
    46201 gatgctgaca aggaaggttt ccttcgcaac gaacgtggac tcatccagac tattggacgt
    46261 gctgctcgta acagtgaagg ccatgtcatc atgtatgcag acacggttac ccagtctatg
    46321 caacgtgcta tcgatgaaac tgcccgccgt cggaaaattc agatggccta taatgaagag
    46381 catggtatcg taccacagac aatcaagaaa gaaatccgtg acctgattgc cgtgaccaag
    46441 gcagttgcta aggaagagga caaggaagtc gacatcaata gcctcaacaa acaagagcgt
    46501 aaagaactcg tcaagaaact agaaaaacaa atgcaagaag ccgtcgaagt gcttgacttt
    46561 gaactggcgg ctcagatccg tgatatgatg ttggaagtca aggccttgga ttaggggaat
    46621 agtatgattt atttaagaaa gttaaagaga gaagatttga tgtctttatg ggaaatggct
    46681 tattcacaac ttaatccagt ttggaaacag tatgatgctc cctattatga tgattatcag
    46741 tatttttcaa attttaaaga attcgaacta caaaaatcag aatccatttt aagcaactca
    46801 aatcgccttg gtatttttgt tgatgataaa ctagttggga ctgtttcgcg ttattgggta
    46861 tgtaaacaaa caagatggat ggaattggga attggtattt atgataaaaa attctggaac
    46921 actggtattg ggaaagttgc tatgttgcag tggatagata ggacgtttca ggattacttg
    46981 gagttggagc atctgggttt gacaacttgg tcagggaatc ttggtatgat gaaacttgct
    47041 gaaaaattaa gaatgaaaaa agaagctcat attccaaaag ttcgttatta tcaaggtaaa
    47101 tattttgata gtattaaata tggtattttg agagaagact gggagaaaat aaatgacggt
    47161 tattatcaaa tcaatggaaa ctcctaaaga gatagaaggt aaatcccttg ttcactggca
    47221 aatgtggaga gaggcttatg atgacctttt gcctgcagaa tttcaggaga caatgacatt
    47281 agaaagatgt cgactcttta gtcaaaagta tccagaaaat acattgattg cgataggtga
    47341 tctgaaggta gttggtttta tcagttatgg taactttcgt gatgagacta tgcaagctgg
    47401 tgaaattatt gctttatatg ttttaaaaga ctattatgga aaaggtatcg cacaaaagtt
    47461 aatgaaagca gctttgactg ctcttaatca tttttctgaa attttcttat gggtattgaa
    47521 agataacaag cgcgccattg ctttctatca aaaaatgggt tttacttttg atggacaaga
    47581 aaaaatactt gaacttggaa agcctataaa ggaaaaacga atggtgttct attctaaata
    47641 attttaaaaa agtaaaagat aatatctctg gtagtaagtc tgaaaattta ataaattaga
    47701 aagcgagtaa atttatgtcc cgttcccaat taacaatttt aacaaatatc tgtctgattg
    47761 aagacctcga aactcagcgc gtggtgatgc agtatcgcgc ccctgaaaac aatcgctggt
    47821 ctggttatgc ctttcctgga ggtcatgtag aaaatgatga ggcttttgcg gagtctgtca
    47881 ttcgtgaaat ctacgaagaa acagggttga ctatccaaaa tcctcaactt gtcggcatta
    47941 aaaattggcc actagataca ggtgggcgct atattgtcat ttgttataag gcgactgagt
    48001 tctctggtag ccttcggtct tcagatgaag gagaagtttc ttgggtgcaa aaagaccaga
    48061 ttccaaactt ggatctggcc tatgatatgc taccattgat ggaaatgatg gaagctcccg
    48121 acaagtcaga gtttttctac cctcgccgta cagaagacga ttgggaaaag aaaatcttct
    48181 agtcttttac taaataacct agctgatcca aggcctcctc gatatagtgg aggtcttgtt
    48241 gtgtttcggc ttcgactagg tggtaatgga tgccatctgt caattcagat agaggtctaa
    48301 agtcagaatg ctcgacttgt tctagaaaat gttgcacatc tctgcgacaa gacagtttaa
    48361 gcaaggtttc aatttctcca taaacgggat ggtcaatcaa ggtattctgg acatgcccac
    48421 cattatcgac aatagcaagg agttcctgac cgatttcttc aacttcatgt ttcactttga
    48481 aaagtttgtg aacataagga ttggtatcaa tttgcttata gatgtagcca cgattggtgg
    48541 ataggatagg agcgccatcg gctctcaaaa ttgcaatatc ctgcacaatg acctgacgtg
    48601 tgacatgaaa atgttccgcc aaactttggc cattaagggc tttaggagct tctttcaaga
    48661 gttggagcag ggcttgtttg cgatcttttg tcatagcttt tccttttaac ggcgttttcg
    48721 aagcacttta tagacagcta gtgctaatgt atagtctacc atactatgga taattgtgcc
    48781 aaatccaact agtacaaata gaacatagaa catattttct acattggtac ccgaagttgc
    48841 gtaaaaaagg acacaggcta atacttcagc aagggcatga acaacagcca aaacaaaatt
    48901 gaaaacccag gaagattttg gtttatctag ggtatcgggg aatttttgta ggtaaagagc
    48961 tcctaaagca ccaaaagata tatgggaaaa agcccgaaaa acgataacca tgggatagcc
    49021 agccatcaaa aatccaaaac tagaggctag gatgacaaaa actgccatca agggcgacaa
    49081 gaacatggct ataaaaatag cgatgtggct ccccaaagta taggaagcag gtggaatgac
    49141 aatcttgaaa ggcataacaa ttggaatcaa aatcgcaata gccgttaaaa gggctgtcat
    49201 tgtcataaat tgtgtctttt tccgtgtatt cacaagaatc tcctttttaa ctgcatatac
    49261 actagtatgg tacaataaac cagacaataa agcaagaatt tacttgggtt tatagatcat
    49321 tttttagtta aaagttatag tagatggaaa ctagaatagt ccacctctac ttctaaaaca
    49381 ttgttagcga tttgactgtc ctgatcgatt tgtcctgttc ttatttcgtt ttactatagt
    49441 aaagatttca ttaaaaagaa actgtataga gcaaaatctc caccttcagg tttgaaagcg
    49501 gagattgttt ttattttttc aaggtttgta gtcgtggaac aatggctagg gttagaattg
    49561 cagaaatgac caattcagca attgaatttg ttgagataac ggttgccaga agaagttgga
    49621 tatttccatt ataaacattt ccaaatagga agaagattcc tccaaggaca aagatagtat
    49681 ttgtcaagga accaagggct ccagctaaaa tcagaccagt cttgtttttc atcagtttat
    49741 agactaagta aggagttaaa ccaatcaaaa tacgtgggac gatggcaatg atagctgagt
    49801 agatgtttcc gtttggtacg aagggagaga agaggtagct tgtcggtaga atcgtaatcg
    49861 tgttaaccgt caagctaagt aatcccatca aaaatccaag tgtaacccca actcgtggac
    49921 cataaataat gctggcaata atgacaggaa tatgaacaat ggtcggtttg attggaaatg
    49981 gaaaaaggtt aaagataagt gagctcagaa agtgtatcac gagcatggta gcaaaaaaga
    50041 tagcaatggg tgcaatatta gagcgttttt tcatcgatag tttcctttat tctttctaaa
    50101 ataattgtga ggtcagctaa agctcctcgt ccgtggtctc cacaagctag tagggattcc
    50161 ttaggagcaa tcagctgata gccgtaggtt tctaatgttt tcagattatt ctgagttact
    50221 ggatggtcat acatttttgt attcatagca ggagctatta gtttgggaat atgacttggt
    50281 agggctagag ctgtactggt taccatgttg tccgcaaatc cgtgagctag ttttgcaata
    50341 gtgttagcag ttgcaggtac cacgataaat aaatctgctt tttttccaag ttcgatatga
    50401 ttgacctgat caggataggg ttccttcatg acatccaagt ggacaggatt ctgtgagagt
    50461 acctgtagtg tcaaaggttg gataaactct gtagcagcct gagtcattaa gacagtgact
    50521 tgatggcctt gtttttttag agaactgact aaatctgccg acttataaga ggcgattgaa
    50581 cccgttacag ccaagagaat gtttgccata gttttccttt ctaagaatga taggcttgaa
    50641 ttttttcaag gaggagttct gcaatttctt ctttagtctg gactgtttga agctgatttt
    50701 tctcaacaaa tatagctcgg tgctgatctg ctgaaatttg agtcaggtca ttcgcgatga
    50761 ttaaatctgc ttgattcttg ataagacttt ttcgtgcaat gtcaaccaga tgatcttcgg
    50821 taacatcaac cagcagtttg aaaccaatca gatgaatagt gggattccat tccttgacta
    50881 gggatatgat tttgggtgtc tttttaagga acaaaacctg aacctcatca gttgaagaaa
    50941 tcttggcctg atgattctgc ttgcttaaaa attcttttag attggagcta gcctgaactt
    51001 cctcaagccc tgtcatataa acaggagtgt agtcagaaac agccattgag tggatcaaga
    51061 cctgataatc ctgaacacgt tcttgcattt ctattagaag gtccttggta ttggtaattt
    51121 ctcgaatact taggttagga tgaggctctg gcttcagagc tcgttttgtc gtaattaaac
    51181 aaacttcatg ccctgcagaa agcaaagtct ctgtgataat tttccccaag tgacctgtag
    51241 aatggttagt gatagagcgg acgctatcga tagcttcact ggtaccgccc gatgtaacta
    51301 aaattttcat agcactattg tacacaaaaa taagcggtaa gtaaaatgaa agtgataaat
    51361 ttttggtaaa aacgaagatt tactatagtt tcaaactata aagccatata aaatttaaga
    51421 aaggactcta aaagccttaa aaacagagat atttacgaac gtttagagag ttttaggatg
    51481 ttaaaaatct tgttttgtga tataatgaat tatcatataa gaggttagag gaattttgaa
    51541 tgaaaacaga tattgaaatc gcacagagta ttgagttgaa gccaattgtt gatgttgtag
    51601 agaaacttgg tatttcttat gacgatttgg agttgtacgg aaagtacaag gctaaactca
    51661 gctttgataa aattcgtgca gttgagagca atccagtcgg taaattgatc ttggttactg
    51721 ccatcaaccc aacacctgca ggtgaaggaa agtcgacgct taccattggt cttgcggatg
    51781 ccttgaacaa gattggcaag aaaaccatga ttgctatccg cgaaccgtct cttggtccag
    51841 tcatggggat caagggtggt gctgctggtg gtgggtatgc acaagttctg ccaatggaag
    51901 acatcaacct ccactttact ggagatatgc atgctattac aactgccaac aatgcccttt
    51961 ctgccttgat tgacaaccac ttgcaccaag ggaatgagct ggaaattgat caacgtcgta
    52021 tcctctggaa acgcgttgtg gacttgaacg accgtgcgct ccgccatgtg actgttggtc
    52081 ttggtggtcc tctaaacggt attccacgtg aggatggttt tgatattaca gttgcttcag
    52141 aaatcatggc aattctttgc ttggcaacgg acatcgagga cttgaaacgt cgtttggcga
    52201 atatcgttat tggttatcgc tatgaccgta cgcctgtttc tgtaggtgat ttgcaggttg
    52261 agggtgcctt ggctttgatt ttgaaggatg ctattaagcc aaacttggtt cagacaattt
    52321 acggtacacc tgcctttgta cacggtggtc catttgccaa tatcgctcat ggctgtaact
    52381 ctgttttggc gacgacaaca gcccttcact tggctgatta cactgttact gaagctggtt
    52441 ttggtgcgga ccttggtgct gagaaattcc ttgatatcaa gacaccaaac ttgccaacat
    52501 ctccagatgc agttgtcatt gtcgcaaccc ttcgtgccct taagatgaat ggtggtgtgg
    52561 ctaaagacgc tctgactgaa gaaaatgtag aggcagttcg tgcaggtttt gctaacttga
    52621 aacgccacgt tgaaaatatc cgtaagttcg gtattccagc agttgtagct attaacgaat
    52681 ttgtatctga tacagaagct gaaattgcag tcttaaaaga actctgtgcc tcaatctatg
    52741 taccagttga attggctagt gtctgggctg atggagcaga aggtggagta gcacttgccg
    52801 aaacagttgt taagacaatt gctgaaaatc cagctaacta taaacgtttg tacgataatg
    52861 ccctttctgt ccaagaaaag attgaaaaaa ttgtcactga aatctatcgt ggtagcaaag
    52921 tgaactttga gaagaaagct caaacacaaa ttgctcaaat cgttcaaaac ggttgggaca
    52981 aattgccaat ctgtatggct aaaactcaat acagtttctc agacaatcca aatgcgcttg
    53041 gagcaccaga aaactttgaa attaccattc gtgaattggt accaaaatta ggtgcaggct
    53101 tcatcgttgc cttaactggt gatgttatga ccatgccagg tcttccaaaa cgtccagcag
    53161 ctctcaacat ggatgttgaa agcgatggaa ctgtactagg cttgttctag tggattgaaa
    53221 ttgactttaa gtgagtttgg aaataatatc caagctcatt ttttctcaga ctgcaagcag
    53281 ttcatcaata agatttatac tcttcgaaaa tctcttcaaa c
//
LOCUS       DBERYS010000009        48822 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.009, whole genome shotgun sequence.
ACCESSION   DBERYS010000009 DBERYS010000000
VERSION     DBERYS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 48822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.009"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(134..2023)
                     /locus_tag="ACL8LA_000617"
     CDS             complement(134..2023)
                     /locus_tag="ACL8LA_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGS1812423.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNFFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2161..3726
                     /locus_tag="ACL8LA_000618"
     CDS             2161..3726
                     /locus_tag="ACL8LA_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS1812424.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3726..4466
                     /locus_tag="ACL8LA_000619"
     CDS             3726..4466
                     /locus_tag="ACL8LA_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1812425.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4575..4790)
                     /locus_tag="ACL8LA_000620"
     CDS             complement(4575..4790)
                     /locus_tag="ACL8LA_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812426.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5661..6911)
                     /gene="ilvA"
                     /locus_tag="ACL8LA_000621"
     CDS             complement(5661..6911)
                     /gene="ilvA"
                     /locus_tag="ACL8LA_000621"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGS1812427.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6961..7317)
                     /locus_tag="ACL8LA_000622"
     CDS             complement(6961..7317)
                     /locus_tag="ACL8LA_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS1812428.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7555..7791)
                     /locus_tag="ACL8LA_000623"
     CDS             complement(7555..7791)
                     /locus_tag="ACL8LA_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812429.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7898..8920)
                     /gene="ilvC"
                     /locus_tag="ACL8LA_000624"
     CDS             complement(7898..8920)
                     /gene="ilvC"
                     /locus_tag="ACL8LA_000624"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS1812430.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8986..9462)
                     /gene="ilvN"
                     /locus_tag="ACL8LA_000625"
     CDS             complement(8986..9462)
                     /gene="ilvN"
                     /locus_tag="ACL8LA_000625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGS1812431.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9455..11155)
                     /locus_tag="ACL8LA_000626"
     CDS             complement(9455..11155)
                     /locus_tag="ACL8LA_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGS1812432.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11355..13022)
                     /locus_tag="ACL8LA_000627"
     CDS             complement(11355..13022)
                     /locus_tag="ACL8LA_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGS1812433.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13025..13390)
                     /locus_tag="ACL8LA_000628"
     CDS             complement(13025..13390)
                     /locus_tag="ACL8LA_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1812434.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13547..13735)
                     /gene="rpmB"
                     /locus_tag="ACL8LA_000629"
     CDS             complement(13547..13735)
                     /gene="rpmB"
                     /locus_tag="ACL8LA_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGS1812435.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13854..14336)
                     /locus_tag="ACL8LA_000630"
                     /pseudo
     CDS             complement(13854..14336)
                     /locus_tag="ACL8LA_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
     gene            complement(14605..16149)
                     /locus_tag="ACL8LA_000631"
     CDS             complement(14605..16149)
                     /locus_tag="ACL8LA_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGS1812436.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16362..16664
                     /gene="gatC"
                     /locus_tag="ACL8LA_000632"
     CDS             16362..16664
                     /gene="gatC"
                     /locus_tag="ACL8LA_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGS1812437.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16664..18130
                     /gene="gatA"
                     /locus_tag="ACL8LA_000633"
     CDS             16664..18130
                     /gene="gatA"
                     /locus_tag="ACL8LA_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGS1812438.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18130..19572
                     /gene="gatB"
                     /locus_tag="ACL8LA_000634"
     CDS             18130..19572
                     /gene="gatB"
                     /locus_tag="ACL8LA_000634"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGS1812439.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19701..20261
                     /gene="efp"
                     /locus_tag="ACL8LA_000635"
     CDS             19701..20261
                     /gene="efp"
                     /locus_tag="ACL8LA_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGS1812440.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20283..20672
                     /locus_tag="ACL8LA_000636"
     CDS             20283..20672
                     /locus_tag="ACL8LA_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1812441.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20665..21087
                     /gene="nusB"
                     /locus_tag="ACL8LA_000637"
     CDS             20665..21087
                     /gene="nusB"
                     /locus_tag="ACL8LA_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGS1812442.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     AVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21136..21330)
                     /locus_tag="ACL8LA_000638"
     CDS             complement(21136..21330)
                     /locus_tag="ACL8LA_000638"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS1812443.1"
                     /translation="MILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFFNFL
                     NHAKPIWIFVYNYIYYHFFR"
     gene            complement(21385..21836)
                     /locus_tag="ACL8LA_000639"
                     /pseudo
     CDS             complement(21385..21836)
                     /locus_tag="ACL8LA_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21854..22039)
                     /gene="briC"
                     /locus_tag="ACL8LA_000640"
     CDS             complement(21854..22039)
                     /gene="briC"
                     /locus_tag="ACL8LA_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGS1812444.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22198..22965)
                     /locus_tag="ACL8LA_000641"
     CDS             complement(22198..22965)
                     /locus_tag="ACL8LA_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGS1812445.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22962..23828)
                     /gene="accD"
                     /locus_tag="ACL8LA_000642"
     CDS             complement(22962..23828)
                     /gene="accD"
                     /locus_tag="ACL8LA_000642"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGS1812446.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23865..25232)
                     /gene="accC"
                     /locus_tag="ACL8LA_000643"
     CDS             complement(23865..25232)
                     /gene="accC"
                     /locus_tag="ACL8LA_000643"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGS1812447.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASRNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25244..25666)
                     /gene="fabZ"
                     /locus_tag="ACL8LA_000644"
     CDS             complement(25244..25666)
                     /gene="fabZ"
                     /locus_tag="ACL8LA_000644"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGS1812448.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(25663..26148)
                     /gene="accB"
                     /locus_tag="ACL8LA_000645"
     CDS             complement(25663..26148)
                     /gene="accB"
                     /locus_tag="ACL8LA_000645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGS1812449.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26151..27386)
                     /gene="fabF"
                     /locus_tag="ACL8LA_000646"
     CDS             complement(26151..27386)
                     /gene="fabF"
                     /locus_tag="ACL8LA_000646"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGS1812450.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27408..28139)
                     /gene="fabG"
                     /locus_tag="ACL8LA_000647"
     CDS             complement(27408..28139)
                     /gene="fabG"
                     /locus_tag="ACL8LA_000647"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGS1812451.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28173..29093)
                     /gene="fabD"
                     /locus_tag="ACL8LA_000648"
     CDS             complement(28173..29093)
                     /gene="fabD"
                     /locus_tag="ACL8LA_000648"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGS1812452.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTSLLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTTHLAHVEDQASLVALLKK"
     gene            complement(29086..30060)
                     /gene="fabK"
                     /locus_tag="ACL8LA_000649"
     CDS             complement(29086..30060)
                     /gene="fabK"
                     /locus_tag="ACL8LA_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGS1812453.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(30179..30403)
                     /locus_tag="ACL8LA_000650"
     CDS             complement(30179..30403)
                     /locus_tag="ACL8LA_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS1812454.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30463..31437)
                     /locus_tag="ACL8LA_000651"
     CDS             complement(30463..31437)
                     /locus_tag="ACL8LA_000651"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGS1812455.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31437..31871)
                     /locus_tag="ACL8LA_000652"
     CDS             complement(31437..31871)
                     /locus_tag="ACL8LA_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS1812456.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31965..32750)
                     /locus_tag="ACL8LA_000653"
     CDS             complement(31965..32750)
                     /locus_tag="ACL8LA_000653"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGS1812457.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33119..34483
                     /locus_tag="ACL8LA_000654"
     CDS             33119..34483
                     /locus_tag="ACL8LA_000654"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGS1812458.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34486..34857
                     /locus_tag="ACL8LA_000655"
     CDS             34486..34857
                     /locus_tag="ACL8LA_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGS1812459.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            35071..36345
                     /gene="serS"
                     /locus_tag="ACL8LA_000656"
     CDS             35071..36345
                     /gene="serS"
                     /locus_tag="ACL8LA_000656"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGS1812460.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVEAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36370..37269)
                     /locus_tag="ACL8LA_000657"
     CDS             complement(36370..37269)
                     /locus_tag="ACL8LA_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGS1812461.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37379..37927)
                     /locus_tag="ACL8LA_000658"
     CDS             complement(37379..37927)
                     /locus_tag="ACL8LA_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGS1812462.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38145..39467)
                     /locus_tag="ACL8LA_000659"
     CDS             complement(38145..39467)
                     /locus_tag="ACL8LA_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGS1812463.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFTFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39558..39646)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39810..42146)
                     /locus_tag="ACL8LA_000660"
     CDS             complement(39810..42146)
                     /locus_tag="ACL8LA_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGS1812464.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKGQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQIGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42250..42798)
                     /locus_tag="ACL8LA_000661"
     CDS             complement(42250..42798)
                     /locus_tag="ACL8LA_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGS1812465.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42795..43097)
                     /gene="zapA"
                     /locus_tag="ACL8LA_000662"
     CDS             complement(42795..43097)
                     /gene="zapA"
                     /locus_tag="ACL8LA_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGS1812466.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43184..44065
                     /gene="rnhC"
                     /locus_tag="ACL8LA_000663"
     CDS             43184..44065
                     /gene="rnhC"
                     /locus_tag="ACL8LA_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGS1812467.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            44069..44683
                     /gene="lepB"
                     /locus_tag="ACL8LA_000664"
     CDS             44069..44683
                     /gene="lepB"
                     /locus_tag="ACL8LA_000664"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGS1812468.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            44917..47283
                     /locus_tag="ACL8LA_000665"
     CDS             44917..47283
                     /locus_tag="ACL8LA_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGS1812469.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47331..48614)
                     /gene="tig"
                     /locus_tag="ACL8LA_000666"
     CDS             complement(47331..48614)
                     /gene="tig"
                     /locus_tag="ACL8LA_000666"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGS1812470.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        14304 a        11200 c         9203 g        14115 t
ORIGIN      
        1 gtgctctaaa atcatcgcca aatcatcaaa ttgaaataag aaatagttta gataaaacta
       61 ttgacttttc ttatagtttg tctttcttct atcttccatt atatcatact ttttcagtct
      121 atggtagctc tctttaaaag gctccgatac tgccgccacc tccgcctcca gagaagccac
      181 cgccagaact tccacttcca gaagatacgg agtaggtact tgctgtattt gcgacactag
      241 cataatggct catttgcgcg cttgaatgat aaaacatact gtgccagcca taagctacat
      301 agagattgat atctggattt tcaacttgaa tctgatgaac cttcatcaaa tgactaacct
      361 tgtccgcata gccaaataag gttgcataga ccaagagacg attccaaaca acaatacttt
      421 ccaattcagc cttatccagt cgtgcaatct cacgtaacat attttcaaaa ctggtccaga
      481 gatagtagac ctcagctccc gcttcattta gaacaccatc acgattatct agtcgaagct
      541 tccaataata gaaaacagac aaaactaacc ctagaaaacc aagtattggc aaagggaggt
      601 aaagatagcc atgaacgtct aaactgtaca agaacaaacc aaatccgata aataggggca
      661 agatagtcaa gacacccata cccacttgca aagtcttttc caaaccagtt aaaggacgat
      721 agtaatctgg gagcccccag aaggaaactc gttttctcac tccttcttgc atctggttca
      781 atacctcttc aaaagaagat ttgagttgaa gccctcttgc ttgaatccgt ttttcatcag
      841 aaactttggc tctacgataa agactatcag ataccttgta atccgcaaaa aaattggaaa
      901 gagtggcttc ttttttgcct gaaaaggcta gatttagaca gtctctctca aatcttgaca
      961 aaccatcttc tttcaccaat ctcaagccaa ctgcatctcc ttctgaaata atagagacat
     1021 tcccacggtc tatcacatct agcaaggtag cttgaataag ttggtcaaaa gtgaatttgc
     1081 cagccccctt gaccaaggga ctcacttcct ccaaggaggt cgagtagact gcttctgata
     1141 aaaccatagg ctctaattcc attggtggtt catagagacg atgatttttg gcatatttga
     1201 ctgaaggagt ggtctttctt ctataaataa aatagaagca gacactcaat aacaaggaga
     1261 tagaaagtat cgaagggagg acccaagtaa cgagttgttt actctgatct ttttctctaa
     1321 caatcgagtc ttctatctta ttaaactctt ctaaacgatt ccctttcaag ccctgatccc
     1381 tagcgctagc aaaatcggtc cgagaccaat aggcatgcaa ctcaactcca cgcttagccg
     1441 gaagattgtc taaacggata gtataatcaa ggttactctt ttcaatcgtt ccctctctaa
     1501 aaagtttccc tgtatggaaa aagagtttct cagccccctt gtctcccctt acatgaaatt
     1561 caaacttttc aatagactct gaactatctg tcagaggttg ccaatttaat tcagcgatat
     1621 catcataaag gaaaagtaaa ttttttaagt tccagacgag gtcaacttca actatgtcgc
     1681 cctcctgacc tggattatag actctcacag tataaccatc cgcttcttct gttacttcgc
     1741 tagtcacatc tgctagttct gcaccgtttt tcgcagcctg aacctttgga tgagggtcaa
     1801 tgtcaaaccc gctaggcatc ttaccagcac gtccaagtcc cacgatttgg cccttaaagt
     1861 cctcctcaaa ctggtaaact atcttctgtc taaactctgc cgtattatct gcatgaatat
     1921 acaaatcccc ttgataagag tttatcttga aatcaatggc aaaaacagag agtggaagaa
     1981 ggcaaaacaa gcctaacacc agtaagaaaa aagttttttt catcaactaa gccccctttt
     2041 tatctttctt atcattatag catcttttcg taagtaaagg cttacttatg ttgaaaaatt
     2101 acgcattttt cgataaaata ggagacagtt attttttaat agatcagaat aggagaaatc
     2161 atgagaaaaa tatacctatc tattttcaca agtctcttgc tgatgctagg acttgtcaat
     2221 gttgctcaag ccgatgaata tttacgcatc gggatggaag cagcatatgc tccctttaac
     2281 tggacccagg atgatgatag caacggagct gtcaaaatcg atgggaccaa tcagtatgcc
     2341 aacggatacg atgttcaaat cgccaagaaa atcgctaagg acttaggtaa agaacctttg
     2401 gttgttaaaa ccaagtggga aggtctagtc cctgccctta cttctggtaa gattgacatg
     2461 attatcgcag gtatgagtcc aactgcagaa cgcaaacaag aaattgcctt ttcgagcagt
     2521 tactacacta gcgaaccagt tttgcttgtc aaaaaagatt ctgcctacgc aagtgctaaa
     2581 tctttggatg actttaatgg tgcgaaaatc acttctcaac aaggtgtcta cctctacgac
     2641 ttgattgcac aaatcccagg tgctaaaaaa gaaacagcca tgggagactt cgctcaaatg
     2701 cgccaagctc ttgaggctgg tgtcattgat gcttatgttt ctgaacgtcc agaagcactg
     2761 actgctgaag ctgcgaactc taagttcaag atgattcaag tagaacctgg tttcaaaact
     2821 ggggaagaag atacagctat cgctatcggg cttcgtaaaa atgacaatcg tattagccaa
     2881 atcaatgcca gcattgaaac catttcaaaa gatgaccaag ttgccttgat ggatcgtatg
     2941 atcaaggaac aacctgccga agctacaaca actgaagaga ctagcagtag tttctttagc
     3001 caagtcgcta aaattctttc tgaaaactgg caacaactct tgcgtggtgc tggtatcact
     3061 cttttaatct ctatcgtcgg aaccatcata ggtctcatta ttggacttgc cattggtgtc
     3121 ttccgtactg ctcctctctc tgaaaacaaa gccatttatg gccttcaaaa actagtcggc
     3181 tgggttctca atgtctacat tgaaattttc cgtggtacgc caatgattgt tcaatcgatg
     3241 gttatctact atggaactgc ccaagctttc gggatcaacc ttgaccgtac actggctgct
     3301 atcttcatcg tttcaatcaa taccggtgcc tacatgactg aaatcgtccg tggtggtatc
     3361 ctagcagttg acaagggaca atttgaagct gcgactgctc ttggtatgac ccataaccag
     3421 accatgcgta agattgtcct acctcaggta gtccgtaaca tcctacctgc aactggtaat
     3481 gaatttgtca tcaatatcaa agatacatct gtattgaacg ttatctctgt tgtcgaactt
     3541 tatttctcag gaaataccgt ggcaacacaa acctatcaat acttccagac atttacaatc
     3601 atcgccgtga tttactttgt cctcaccttc accgtaacac gtatcctacg atttatcgag
     3661 cgccgaatgg acatggatac ctatactaca ggtgctaacc aaatgcaaac ggaggatttg
     3721 aaataatgac acaagcaatc cttgaaatta aacacctcaa aaaatcctat ggacaaaacg
     3781 aagtgctaaa agacatttca ctcactgtcc acaagggaga ggtcatctct atcatcggaa
     3841 gctctggaag cggaaaatcg accttcctac gctccattaa cctacttgaa acaccaactg
     3901 atggacaaat cctttatcat ggacaaaacg tcctcgaaaa aggctatgac ctcacgcaat
     3961 accgtgaaaa gttggggatg gttttccaat cctttaacct ctttgaaaat ctcaatgttc
     4021 ttgaaaacac aatcgtcgct cagacaactg tcctaaaacg cgaacgcaca gaagctgaaa
     4081 agattgccaa agaaaacctg gaaaaggtcg gcatgggaga acgctactgg caagccaaac
     4141 caaaacaact ctcaggtggt caaaaacaac gtgtggccat cgctcgtgcc ctctccatga
     4201 atccggacgc tattctcttt gatgaaccaa catcagctct cgatccagaa atggttggag
     4261 aagtcctcaa aatcatgcag gacctggctc aggaaggctt gaccatgatt gtcgtaaccc
     4321 atgaaatgga atttgcccgt gatgtctctc accgtgttat ctttatggat aagggcgtga
     4381 tcgctgaaga aggtaaacca gaagacctct tcaccaatcc taaagaagac cgaacaaaag
     4441 agttccttca acgctatctc aaataaaaag aaaaggctgc atcaaccgtg cagtcttttt
     4501 gctgtcctca aaatgaaaag gcagattctc tatctctact acttgaaggt caagaatccg
     4561 ccgttatcca aagattaatc ttcaaatttt tcataatttt tttcatagaa atcaattaag
     4621 cctagggctg tttcaaatga aatatttttt acttttgcac gaccctgcgc cagagcaatg
     4681 atagacattt cacgcgcatt cgtttcttta gagatacggt agcctgtgat tttcttatca
     4741 cgaacccagc caacaactga ttctactttt tcaaagtttg acttagccat gtttttctcc
     4801 tattttcttc tttataatat ttaattgtat acgtttttac tacttttgtc aataaaaaaa
     4861 acatatattc aacgaaaata attagttcag aattttctta cttccttttt aaatccgata
     4921 atatataggt tttgctagcc tctacaccat aaatatttgc taataaaact actgtattat
     4981 taccctctta aggtgcgaaa ttattcaagt tcttaaaaaa tgcttctcta tttccgtaag
     5041 atagcttatt tcttcgattt aagaactgaa gatgatagtt ctaaccttta gaaatccaag
     5101 ccttcactag ctcaaaaact cctatctttc ttcctcaata aatcatctga gagataggta
     5161 tttcttatat ccttcaaatt catcactcct ttggatattg aggtgctagc ctagaagcag
     5221 acacccgatt atctaaacca tccttaatag agatgacaag acagataagc tcagttaatt
     5281 acttatatat ctttttaata tggtatattt gtcaacaaaa agaaatcaaa tcatacctta
     5341 tactcaatga aaatcaaaga gcaaactagg aagctagccg caagttgctc aaaacactgt
     5401 tttgaggttg cagatagagc taacgtggtt tgaagagatt ttcgaagagt attaaagaca
     5461 gtgtgactat gcttgtcaat agataggaaa tcaaaatgaa ggatggaaat ctgagccaaa
     5521 catcataagt gctataatga acagaagtaa gaattgcacg ataagttttc taaaaccata
     5581 ggaatcgtcc cctttcttca tcttcattat agcatatata catcagttgt acaaataata
     5641 tgatattttt ttattagtcc tcagacaagc atattataaa gcgtttcatt accatttaag
     5701 ttaatataag ctggatcaaa accttccatt ctacgaatca aacctgcata atcatgctta
     5761 tctgctaaag cgatcccaat caatactggg cctgttccct tgctagctcg tttgatatac
     5821 tcaaaacgtg tgatatcatc atttggcccc aggatatcat ttacaaactc acgcaaagct
     5881 cctggacgtt gtgggaaatt gaccacaaaa taatgtttga taccatcata aatcaaggca
     5941 cgctcttcca tttctggcat acggttgata tcattatttc ctccagaaat gatacaacaa
     6001 atggttttcc ccttaatata ttcagctaaa acctctaaag aggcgatact agctgctcca
     6061 gcaggttctg cgactatccc ttgcttagag taaaggtcaa tcaaggtttc agaaatcaat
     6121 ccctcatcga cacctaccaa agtttgaata tgttgacgag ttgcttcata ggtcaactga
     6181 cctacctttt gcacagcaat cccatccgca aatttatcaa tttccttgag tttgactgga
     6241 cctccagcct caaaggcagc tttcatggaa cgcgctccat tcgcctctac tccgataacc
     6301 tcaatctctg gacttgtttc cttgatatag gtagaaaccc cggcaatgag accgccacca
     6361 ccaacaggaa ccaagacagc atcaaaatca atcgattctt ttcgagcttc ttctaaaatc
     6421 tcataagcaa ctgttccttg acctgcttga acatgagcat catcaaaagg atcaataaag
     6481 gtacgatttt cagagactgt aaattcttga gctgctttgg ctgaggcatc aaaggtatct
     6541 ccaactagtt taatagttac aaaatcccca ccaaaaaagc gaacctgacc aatcttttgt
     6601 tgtggcgtag taatgggcat aaagatagta gcaggaattt tcatttcatt acaagtatag
     6661 gctactccct gcgcatgatt tcccgcagaa gcgcaaacta ccccacgttc acgttcttcc
     6721 ttgctgagct gggaaatggc ataataggca ccacgaattt taaaggagcg aacacgctgg
     6781 gcattttctt ttttcaaata aatcttagca ccatacttct ccgataaata atgatcgtaa
     6841 tccagtggag tattcacaac cacaccgttc aagaccttgt gagccttgat tatatctttt
     6901 gaacttaaca tataaaacct ctttttctat aaaaaacagt cactaatgac tgttttttag
     6961 ttattcatct ccgaaaattt cagtaacact ttcatggtat ttatgagcca tacgaatcga
     7021 aaactcttta ggaagttcat aacgatttgt ggaaatccaa tcatataacc attctaccaa
     7081 actttcttca ctatcattac gatggaagat aaaatagttt aaaatttctt tatattctac
     7141 atcattcaaa tccaacgtgt ctacaaattg actacgagga acaaaattag aatctaattt
     7201 tcttagttga tctgtcaatt ctcccaattg taaatccaac tgaaaaaata gtttttgcag
     7261 ttgcctatca gtttcatcac atcctgtctt gatagaaaac aaattggtaa tattcatatg
     7321 atttcaaccc ctaaataagt acatctaata tcccacgaat agctgaagct aaagttttcg
     7381 cagtccagtc atttaaaccc gattctttca atccagatgc aataatttgc tctatttgat
     7441 ataaaccgcc atctatcgtc tcatttattc tgcgaagagg accttcaata taggataaag
     7501 cttctccgaa tctaacagct gatttaggtc ctaatgattt accaactata tttgttaata
     7561 ttttttattt ctcaaaatcc atttcgctat ataacgagca gatttagtcg caccagctct
     7621 ctcaaacgga gaatgaattt caagaccttc ttctctagct atttgtgcca atttgaagaa
     7681 ttgttcaaag gtcatatgca acctaccatc ttcaaaatta gcatcatcat aaatgttaat
     7741 tacagcattt tcatcatacg accaatctgc tttattttgg tctgctagca ctattaccga
     7801 cggtgtaatt acattataca actagaacca ataataacga tgcaattttt ctcataatct
     7861 ttttcataaa atcatctcca gcgctatata tttctaatta gttatagatt ttgaatgcat
     7921 catcgtcgtt tttaccaacg aatggcattg ctttacgcaa ttctgcacca actttttcaa
     7981 tttcaaggtt agctgcttgt tcacggtaag cagtcaattt tggacgtcca gctttatagt
     8041 catttacaaa gtcatttgca aatttaccat tttggatgtc tgccaagaca gccttcatat
     8101 tttctttaac ttgttcagtg attacacgtg gacctgatac atagtcaccg tattcagcag
     8161 tgtttgaaat agattgacgc attttcttga atccaccttc gtagatcaag tcaacgatca
     8221 atttcatttc gtgaagaact tcaaagtaag ccaattctgg agcgtaacct gcttctgtca
     8281 agacttcgaa acctgcttcg ataagggcag tcaaaccacc acaaagtaca gcttgttcac
     8341 caaacaaatc ttcttcagtt tcttctttgt aagttgtttc aagaagacct acacgagccg
     8401 ctccaacacc tttacaccag tccatagcaa tgtttttagc atttcctgtt gcatcttggt
     8461 atactgcata aagagctgga acaccaaatc cttcttcgta agtacgacgt accaagtgtc
     8521 ctggtccttt aggagcacac atgaagacat ctacatccgc aggaactttg ataaattcaa
     8581 agtggatgtt gaaaccatgg gcaaatccaa ctgcgtttcc agcttccaag tttggagcga
     8641 tttctgcttc gtacaattct tgttgaattt cgtctggcgc caagatcatg ataacatcag
     8701 ccaacttagt agcttctgct actgtgtaag tatcaaatcc atcttctttt gctttatcaa
     8761 aagatttacc tggacgtaca ccgataataa cgtcacgacc tgaatcacgc aagttttgag
     8821 catgcgcatg cccttgtgaa ccataaccga taacggcgat ttttttaccg tcaagtgctg
     8881 ctactttaac atctttttca tattccattt gaactgtcat agcttttctc tcttttctat
     8941 tatttattgc cttttaagct ggtttaacaa atttaagttg gatttttaat cgcgggtaaa
     9001 tccagttgca cccgttcgag caatattgcg aataccgtat gggcgaatga ctcgcaatag
     9061 ggcttcgctc ttttctgcat ttcccgtcat ctgaatggta atcgagcttg gcgctacgtc
     9121 taccactgtt gcacggaaag gttgaataat cgctaaaatc tcagctctct tctcagctgg
     9181 cgctgacatc ttaaccaaaa tcacctcgcg ctccaaatga ggcttgtctg taatatctcg
     9241 aatgcgaatc acatcaatct gacgattgag ctgtttgatg atttgctcca cttcatcatg
     9301 agaagcaaca tcaataataa tagtgatacg cgatacattc ggatcttctg ttgctccaac
     9361 agagatgctt tcaatattaa cctgacgacg agataggaca cctgtaaagc gattgaggac
     9421 tcctgaacga ttttgtagtt ttgctgttaa cattctacgc atggaactgc acccccaaca
     9481 tctcatgatt actcttacca gccggtacca ttggtaacac ctgttcctta cgagaaatat
     9541 ctacctcaat tagcatagga acatcctcag tgatgacttc aaggtcttga gccaaggtct
     9601 caggattgtc aaacttatag tttttaatac cataagcctg cgccatcaat tggaaatcag
     9661 gaagggtatc aaagaccgac tctgatgttc tgccttcata gaaggattcc tgccactggc
     9721 gaaccattcc gagtgaatga ttgttcagca taaccacctt gattggcacc ttgtaaatat
     9781 tcaaaatagc caactcctgg ttggtcattt ggaaaccacc atccccaaca aacaagacta
     9841 cttccttatc tgggttagca attttagcac cgattgctgc tggaattcca aagcccattg
     9901 ttcccaaacc acctgaagtc actaactgac gttcattttg gtagggataa tactgagctg
     9961 tccacatttg gtgttgacca acgtctgtta ccacaatggc atctccattc gtcaattcac
    10021 caattcgttc aataactgct tgcggttgaa ccacacgctc tttcttgtca tacgaacgaa
    10081 cacgattctt gtctttagtg actttctcaa tccacttttc agtattgttg tgaactgttg
    10141 gttctgctag caacatttgc aaggccttct tagcatctcc aactacagga atgtctgcac
    10201 tgataatctt gccaatctca gctgggtcaa tatcaatgtg ggcaacctta gcattcttag
    10261 cgaaagtctt aggattcccc gtcaaacggt catcgaaacg agaaccaata ctaatcataa
    10321 agtccgcttc cgtcatggca atatttgctg cgaatgaccc gtgcatgcct cccattccaa
    10381 gaaagagtgg gtgactcgtt gcaatcgttc cttgtcccaa aagactggtt accactggaa
    10441 tttgatagcg ttctgcaaat tcatttagtt ccgtagcagc ctcagcataa ctaattccac
    10501 caccagctaa caagactggc tttttagcct tggacaattg cttcaagatt ttcttgattt
    10561 gcatatcatt cggctcaaga gtcggctgat aacttggtaa attcacttct ggtgaataaa
    10621 tgaagtctgt ttctaaagca gatatgtctt ttggtaggtc aattacaact ggccctggac
    10681 ggcctgtagt tgcgatatgg acagcttccg taatgatacg cggaatatca gctgtctcac
    10741 gaacttggta attgtactta gtgattggca tggtaattcc tacgatgtct gcctcctgaa
    10801 aggcatcctt cccaatccct gctcgcgcca cctgacctgt aaagaccaaa aggggaacgc
    10861 tatcgctcat agcatccgca atccctgtaa tggcatttgt tgctcctggt ccactagtga
    10921 cgacggcaac acccaacttt ccagttgatt tggcatagcc ttcagcttca tgcaaacaac
    10981 cttgctcatg gcgccctaga atgtggcgaa tgcctttaaa attatatatc gcatcataaa
    11041 aaggcaaaac cgcaccacca ggataaccaa agatggtatc aactcctaaa tcacgaagtg
    11101 tttccaaaac taggtccgac cccgtcttag gagattctaa actgattttc tccattattc
    11161 ccctttcttt cctcttaaaa ataacttgtt actatcatac cattttttca aaatttttca
    11221 agataaaaga agaaattttc tgaattttct attttaacgt ttatttatga atgttatttt
    11281 tgtttttatt aaaaagatac cgatttcatt tactaaaaag aaaaaagaac tgatttctca
    11341 gtccttcatt aatcttattc cacactaaat aggtatgggt aaacaggttg ttgaccttgg
    11401 tgaatctcga cttcaacgtc ttcgaattct tctacgattt cttgagcgat ttcattggca
    11461 agttcttcgc ttccgtcttc accgacatag aaggttacga tttcactgtc ttcatccaac
    11521 atatgtttca aggtttcagt caatgtttgg tgcatatcag ggtttgacac aagaattttt
    11581 ccatccacca tacctaaatt atcgttttca tggatttcta agccatcgat cgttgtatca
    11641 cgcacggctg ttgtgacgct tccgctaacg acatcgctaa gagcagctgt catacgctct
    11701 tggttttctt caatggactt gcttggatca aaggcaagaa gacttgtcat accttgagga
    11761 agagtgcgag cctctaccac taccgctggt tgctccaaaa cttctgccgc agattgagct
    11821 gccatgaaga tgttcttgtt gtttggcaag aagatgatgt tacgggcatt aacctgttca
    11881 acagccttga taaagtcttc tgttgaaggg ttcatggttt gaccgccttc gataacataa
    11941 tccacgcctt gagaacagaa gatatctgct agacctttac cagccaccac agcaatcaaa
    12001 gcatactctt tttcttcagc cgacttgata acttgagtag cttctttctc aacctgtgct
    12061 tcgtgttggt tacgcatatt gtcaactttt accttgacca agctaccata tttgagacct
    12121 tcttgcataa caagtcctgg atcttctgta tgaacatgga ctttgacaat ttcatcatcg
    12181 ttaacaacga ggagagaatc tccaagctca tccaagtagt tacggaattc atcgtagtca
    12241 aaatctttag cataggttgg accttgctta agagctacca tgatttcagt acagtaacca
    12301 aacgtgatgt cctcagtcgc tacgtgacca gctacagact tatgatgctc tacattgatc
    12361 atctcactca tgttggcagg agtcgctaca aagtcctcag atgcaatata ttcgccagta
    12421 agggctgaaa ggaaaccttc gtagatgaag accaatcctt gaccacctga gtccacaacg
    12481 ccaacttctt tcaatactgg aagcatgtct ggtgttttag ctagagctgt tttagcacct
    12541 tccaaggctg cgcgcatgac ttcaacagtg tcatctgttt gctcagcttt tttcttagca
    12601 ccgatagcag ctccacgaga aactgtcaaa atcgttcctt caacaggttt catcactgcc
    12661 ttataggcaa cttccacacc tgattggaag gccagagcca agtcttgacc tgttaactcg
    12721 tctttatcct tgatagcttg ggaaaatcca cggaaaagct gagacgtaat cactcctgag
    12781 ttcccacgcg cacccatcaa aagccctttg gcaagaatgc tcgctacttc tccaactgta
    12841 gaagctggct tgtctgcaac ttctttagca ccattttcaa tggtcattcc catatttgtc
    12901 ccagtatctc catctggaac tggaaagacg tttaatgaat tgacatattc agcttgctta
    12961 ttcaagcgag ttgatgcagc ctgcaccatt tcttgaaata agctagtagt aatttttgac
    13021 acggttattc tcctacaact ttgatatttt gaatgtagac atttacagtc tgagcagtaa
    13081 ttccaagctg gttttccaag ctaaaggcaa cacgctcttg aatgtttttt gacacttcac
    13141 taatctttgt tccgtagctt aacacggtat atacatcaac tgcaatactg ccatcttcgg
    13201 ctgcctttac gacgacacct ttagaataat tttccttacc tagcagggct tggaaattat
    13261 ctttgagggc atttttacta gccataccga ccacaccaaa aatctcagtt gctgctccac
    13321 ctacgacggt tgcaatcact tcatctgtta gttcgatttg accatctttt gtattaattt
    13381 ttacagtcat cctttttacc tcaactagtt gatactctat tttatcatat ttcagcccaa
    13441 gtgtaaaagc agaatgatgt atcagcggat atttactcta tttttcaaac gatgttatac
    13501 ccacaataaa agaaaaaaga ccctaaggtc tcctttgctt ttattattaa acgcgttcaa
    13561 ctttacctga tttcaaagca cgagctgaag cccaaacttt tttaggttta ccatcgataa
    13621 gaacagtaac tttttgaagg tttggtttta cggcacgttt tgtttggttc atcgcgtgtg
    13681 aacggttgtt tcctgataca gtcttacgac ctgtaaagta acatacttta gccattgtgt
    13741 tttcctccta ttagatctaa tatagcggat gtgctagcac cacataccgt actatgttat
    13801 cacactttct tgttttttgc aagggaattg gaagattttt tatttgtgtc ttaaatcagg
    13861 tcttgcgtga cattttctgc tctccacatg ccattgttga ttaacagaac accagaatta
    13921 aaattatgtg tataaaaatc atctctaact gcagctaagg gtatagccgt caagtccaaa
    13981 tcccacagct catctatcga ttttcttaca acaatatctg aatccaaata cagtacacga
    14041 gactcgctta catactttgg aataaaatac ctaaaaaaag ccgcatatga aagtccctca
    14101 aaggggagac gataaccttt cagaatacta ctgtcaatct gaacattcac aatctcacta
    14161 ttcaaagtct ctagtctttt ttccatcaat tggaaccatt ctcgcggaag gtcatcatta
    14221 aaaacataaa acttaagatt ataatgatga acacaaagag attttattgt tgtttcaact
    14281 ttatccatat aagcattatc tgcacctaag acaatcgctt ttttctcttc tttcactctt
    14341 tatcttattt ctttttattc ccatcatatt attcccatca tatgtttcta cgtaaccatt
    14401 attttcgcct attcgttcgt aaaaccatac cagtggagat tttagatgaa gtcccattac
    14461 ggtttacaat ttttacatta cgacacggag ttttacaaat cgatttcatt tgccaaacgt
    14521 agttagtgag gcagttagct agttcgccaa atagcgacta gcgtccaaca atttggaact
    14581 ttagctccaa ttgttggtac tgagtcacat cttctcctct aactctacgt ctggatactt
    14641 gtccgcaaac cagcggaggg caaagtcatt ttcaaagaga aagactggtt ggtcaaaacg
    14701 gtctttggct aagatattgc gacttgacga catccgttca tccaagtcct caggcttgat
    14761 ccaacgaacg gtctttttac ccattgggct catgaccact tccgcattgt attcgccttc
    14821 catacggtgt ttaaagactt caaactggag ttgaccaaca gctcccagca tgtactcacc
    14881 tgtttggtaa ttcttataaa gctgaacggc tccttcttgc accaattgct caatcccctt
    14941 gtggaaggat ttttgcttca taacattctt ggcagaaact ttcatgaaaa tttcaggagt
    15001 aaaggttggc agtggttcaa attcaaactt gttttttcca accgtcaagg tatccccaac
    15061 ctgataagta ccggtatcgt aaaccccgat aatatcacct gccacggcat tgatcacatt
    15121 ctcacgactt tccgccataa actgggtaac attagatagt ttagccccct taccagtacg
    15181 agggagattg acactcatgc cgcgctcaaa ttcgcctgat acgatacgga caaaggcaat
    15241 acggtcacgg tgacgaggat ccatgttggc ttggatttta aagacaaagc ctgagaaatc
    15301 cttgtcataa ggatccacaa tttcaccgtc tgttttcttg tgaccatgtg gttctggagc
    15361 aaacttgagg aagatttcaa ggaaggtctg cacaccaaag tttgtcaggg ctgaaccgaa
    15421 aaagacaggc gtcaattctc cagccagaat agcttcctct gaaaactcat tcccagcttc
    15481 atttaaaagc tcaatgtcat ccttgacttg ctcgtagaaa ggattgctac caaagagttt
    15541 gtccccgtct tctagactgg caaaacgctc atcccctttg taaagctcta aacgttggtt
    15601 atagaggtca tacaagccct caaaggcttt ccccatcccg ataggccagt tcatagggta
    15661 gctagcaatg cccaagattt cttccaattc ttgcaagaga tccaaaggct cacgaccgtc
    15721 acggtccagc ttgttcataa aggtaaaaac tggaatgcca cgatgtttca caacctcaaa
    15781 caatttcttg gtttgagcct cgatcccctt ggcagagtcc acgaccatga ccgcagcatc
    15841 caccgccatc aaggtacgat aggtatcttc tgagaagtcc tcgtgccctg gcgtgtctaa
    15901 gatattcacg cgcttgccgt cgtagtcaaa ttgcataaca gatgaagtaa cagaaatccc
    15961 acgttgcttc tcgatatcca tccagtcaga tttagcaaaa gtccctgttt tcttcccttt
    16021 taccgtacca gcctcacgaa tctcaccccc aaagtagagt aactgctcag tgatggttgt
    16081 tttccccgcg tccgggtggg agataatggc aaaggtacga cgtttcttaa tttcttcttg
    16141 aatattcata agttctcttt ctttgattct ctatttttct tgtttcaata gctgagaatg
    16201 atttttacat tggattttac cattcctttc aatactccat tatatcggat tttagcattt
    16261 ttttcaattt ctatctcttt tcacttcccc ctcccttatt tataggaaaa tatggtaaaa
    16321 tagaacagac taaaaatcat catttcacga aaggatgcaa gatgaaaatt acgcaagaag
    16381 aggtaacaca cgttgccaat ctttcaaaat taagattctc tgaagaagaa actgctgcct
    16441 ttgcgaccac cttgtctaag attgttgaca tggttgaatt gctgggcgaa gttgacacaa
    16501 ctggtgtcgc acctactacg actatggctg accgcaagac tgtactccgc cctgatgtgg
    16561 ccgaagaagg aacagaccgt gatcgcttgt ttaaaaacgt acctgaacaa gacaactact
    16621 atatcaaggt gccagctatc ctagacgatg gaggagatgc ctgatgactt ttaacaataa
    16681 aactattgaa gagttgcaca atctccttgt ctctaaggaa atttctgcaa cagaattgac
    16741 ccaagcaaca cttgaaaata tcaagtctcg tgaggaagcc ctcaattcat ttgtcaccat
    16801 cgctgaggag caagctcttg ttcaagctaa agccattgat gaagctggaa ttgatgctga
    16861 caatgtcctt tcaggaattc cacttgctgt taaggataac atctctacag acggtattct
    16921 cacaactgct gcctcaaaaa tgctctacaa ctatgagcca atctttgatg cgacagctgt
    16981 tgccaatgca aaaaccaagg gcatgattgt cgttggaaag accaacatgg acgaatttgc
    17041 tatgggtggt tcaggtgaaa cttcacacta cggagcaact aaaaacgctt ggaaccacag
    17101 caaggttcct ggtgggtcat caagtggttc tgccgcagct gtagcctcag gacaagttcg
    17161 cttgtcactt ggttctgata ctggtggttc catccgccaa cctgctgcct tcaacggaat
    17221 cgttggtctc aaaccaacct acggaacagt ttcacgtttc ggtctcattg cctttggtag
    17281 ctcattagac cagattggac cttttgctcc tactgttaag gaaaatgccc tcttgctcaa
    17341 cgctattgcc agcgaagatg ctaaagactc tacttctgct cctgtccgca tcgccgactt
    17401 tacttcaaaa atcggccaag acatcaaggg tatgaaaatc gctttgccta aggaatacct
    17461 aggcgaagga attgatccag aggttaagga aacaatctta aacgcggcca aacactttga
    17521 aaaattgggt gctatcgtcg aagaagtcag ccttcctcac tctaaatacg gtgttgccgt
    17581 ttattacatc atcgcttcat cagaagcttc atcaaacttg caacgcttcg acggtatccg
    17641 ttacggctat cgcgcagaag atgcaaccaa ccttgatgaa atctatgtaa acagccgaag
    17701 ccaaggtttt ggtgaagagg taaaacgtcg tatcatgctg ggtactttca gtctttcatc
    17761 aggttactat gatgcctact acaaaaaggc tggtcaagtc cgtaccctca tcattcaaga
    17821 tttcgaaaaa gtcttcgcgg attacgattt gattttgggt ccaactgctc caagtgttgc
    17881 ctatgacttg gattctctca accatgaccc agttgccatg tacttagccg acctattgac
    17941 catacctgta aacttggcag gactgcctgg aatttcgatt cctgctggat tctctcaagg
    18001 tctacctgtc ggactccaat tgattggtcc caagtactct gaggaaacca tttaccaagc
    18061 tgctgctgct tttgaagcaa caacagacta ccacaaacaa caacccgtga tttttggagg
    18121 tgacaactaa tgaactttga aacagtcatc ggacttgaag tccacgtaga gctcaacacc
    18181 aattcaaaaa tcttctcacc tacttctgcc cactttggaa atgaccaaaa tgccaacact
    18241 aacgtgattg actggtcttt cccaggagtt ctaccagttc tcaataaagg ggttgttgat
    18301 gccggtatca aggctgctct tgccctcaac atggacatcc acaaaaagat gcactttgac
    18361 cgcaagaact acttctatcc tgataacccc aaagcctacc aaatttctca gtttgatgaa
    18421 ccaatcggat ataatggctg gattgaagtc aaactagaag acggtacgac caagaaaatc
    18481 ggtatcgaac gtgcccacct agaggaagac gctggtaaaa acacccatgg tacagatggc
    18541 tactcttatg ttgacctcaa ccgccaaggg gttcccttga ttgagattgt atctgaggca
    18601 gatatgcgtt ctcctgaaga agcctatgct tatctgacag ccctcaagga agttatccag
    18661 tacgctggca tttctgacgt taagatggag gaaggttcga tgcgtgtgga tgccaacatc
    18721 tcccttcgtc cttatggtca agagaaattc ggtaccaaga ctgaattgaa gaacctcaac
    18781 tccttctcaa acgttcgtaa aggtcttgaa tacgaagtcc aacgccaggc tgaaattctt
    18841 cgctcaggtg gtcaaatccg ccaagaaaca cgccgttacg atgaagcgaa taaagcaacc
    18901 atcctcatgc gtgtcaagga aggggctgct gactaccgct acttcccaga accagaccta
    18961 cccctctttg aaatttctga cgagtggatt gaggaaatgc ggactgagtt gccagagttt
    19021 ccaaaagaac gtcgtgcgcg ttatgtatct gaccttggtt tatcagacta cgatgctagt
    19081 cagttgactg ctaataaagt cacttctgac ttctttgaaa aagctgttgc cctaggtggt
    19141 gatgccaaac aagtctctaa ctggctccaa ggggaagtcg ctcagttctt gaatgctgaa
    19201 ggtaaaacac tggaacaaat cgaattgaca ccagaaaact tggttgaaat gattgccatc
    19261 atcgaagacg gtactatttc atctaagatt gccaagaaag tctttgtcca tctagctaaa
    19321 aatggcggtg gcgcgcgtga atacgtggaa aaagcaggta tggttcaaat ttcagatcca
    19381 gctatcttga tcccaatcat ccaccaagtc tttgccgata acgaagctgc tgttgccgac
    19441 ttcaagtcag gcaaacgtaa cgccgacaaa gcctttacag gattccttat gaaggcaacc
    19501 aaaggccaag ccaacccaca agttgccctt aaactacttg cacaggaatt ggcgaagttg
    19561 aaagaaaact agacagaaca aaaccagccc taaggttggt tttttcttct ctaccaactc
    19621 ccaataacta ttttggcttt atttccagag tattttatgg taaaatgaag agtaataata
    19681 tttattaaag aggtaaaaac atgattgaag caagtaaatt aaaagctggt atgacctttg
    19741 aaacagctga cggcaaattg attcgcgttt tggaagctag tcaccacaaa ccaggtaaag
    19801 gaaacacgat catgcgtatg aaattgcgtg atgtccgtac tggttctaca tttgacacaa
    19861 gctaccgtcc agaggaaaaa tttgaacaag ctattatcga gactgtccca gctcaatact
    19921 tgtacaaaat ggatgacaca gcatacttca tgaatacaga aacttatgac caatacgaaa
    19981 tccctgtagt caatgttgaa aacgaattgc tttacatcct tgaaaactct gatgtgaaaa
    20041 tccaattcta cggaactgaa gtgatcggtg tcaccgttcc tactactgtt gagttgacag
    20101 ttgctgaaac tcaaccatct atcaaaggtg ctactgttac aggttctggt aaaccagcaa
    20161 cgatggaaac tggacttgtc gtaaacgttc cagacttcat cgaagcagga caaaaactcg
    20221 ttatcaacac tgcagaagga acttacgttt ctcgtgccta atctctagaa agaggtcatt
    20281 ctatgggaat tgaagaacaa cttggcgaaa tcgttatcgc cccacgtgta cttgaaaaaa
    20341 tcattgctat cgctactgca aaggtagagg gtgttcactc tttttcaaac agatcagtgt
    20401 ctgataccct ttcaaaactt tcactcggcc gtggcattta tcttaaaaac gtggacgaag
    20461 aactcacagc agatatctat ctctaccttg agtacggagt aaaagttcct aaggtagcgg
    20521 ttgctatcca gaaagctgtc aaagatgccg tccgtaatat ggctgatgta gaactcgctg
    20581 ctatcaatat tcacgttgca ggtatcgtcc cagataaaac accaaaacca gaattgaaag
    20641 atctatttga cgaggacttc ctcaatgact agtccactat tagaatctag acgccaactc
    20701 cgtaaatgcg cttttcaagc tctcatgagc cttgagttcg gtacggatgt cgaaactgct
    20761 tgtcgtttcg cctatactca tgatcgtgaa gatacggctg tacaacttcc agcctttttg
    20821 atagacctcg tttctggtgt tcaagctaaa aaggaagaac tagataagca aatcactcag
    20881 catttaaaag caggttggac cattgaacgc ttaacgctcg tggagagaaa cctccttcgc
    20941 ttgggagtct ttgaaatcac ttcatttgac actcctcagc tggttgctgt taatgaagct
    21001 atcgagcttg caaaggactt ctccgatcaa aaatctgccc gttttatcaa tggactgctc
    21061 agccagtttg taacagaaga acaataaaaa gaaaaagtgt ttgatatcta tcaaacactt
    21121 tttctttgcc tcccactatc taaaaaaatg ataatagata taattgtaaa caaaaatcca
    21181 gataggtttt gcatgattga gaaagttaaa aaaactatgg cagagaatcg ttaatctcag
    21241 attgtcggta gaacgataaa caagggcaaa aaagaaacca atcagactat aatataataa
    21301 actaattgga tctctgtgag atagtatcaa atgactaagc ccaaagataa ggcctgataa
    21361 gatgacatct aaataatact tggactaggg aaagaaggta ttcataaaat accctctatc
    21421 aagagtctcc tcaaaaacag gaccgatgat tacaggcagg acaaaagata agatagtcga
    21481 taaaaaggtt ggttgtccat ttgaaagaac cacggtaaaa tactcatcat gaatattcct
    21541 atgattaatc aaatgagcat agcgtgccca aaaattaccg agaatctgat aaaccacata
    21601 agttgcaaat aaggagaaga caaatgacca gttccagctc tttttctttt ttaacctcca
    21661 aattaataga aaaggaaact tcccactaat cccactgtta aaataagaga atagacatca
    21721 gctcctaacc ctaaaatgat cgtcacatac aatccaattg tttgtggtaa ataggtagat
    21781 agtaaaataa taagcaaaaa tattccaaat tgtcttagtt tctttgtgtt tctcatcgta
    21841 cttttttgaa agattaccct gttcggaagc cgtacttcca agcatctata taagaattaa
    21901 gtgccccttg cctcatatag ggagcaaatt ctctataata taaccatcta ctatatccat
    21961 cttcccaaac agcaagacca cctgaagttt gctccaagtc ctcagttgaa ataactgtaa
    22021 atgtttctgt acctgtcatt gcaagtacct tcttaaaata gattgttgta ggctcacatt
    22081 tatagtatat ttcttttttt gtctatttta tagtctatct cctcaactgg caatttttcg
    22141 acctgaattg catttttcca taaaaaatga gacctttcta gtctcattta gtcattctta
    22201 gtattttcta aatcgttgat agcgttcttc cagcaactct tctagcggtt tttgtgaaag
    22261 tctagccagc tccgtttgga gttctttttt gacactctta attagttctt tactagaaag
    22321 tcctacttca gaaatcacct tatccaccac gtccatttct aacagttcat gcgaagtgat
    22381 tttcatcagt tctgctgctt ccatggcgcg agtaccgtcc ttccataaaa tggaagcaaa
    22441 gccttctgga ctgagaatgg catagataga attttccagc atccagacac ggtccgcgac
    22501 agctagagcc agagccccgc ctgaaccacc ttcaccgata ataatggcga taataggaac
    22561 tttcaggtca ctcatttcca tgagattgcg agcgatagct tccccttgac cacgttcttc
    22621 cgctccgaca ccaggataag cacctgctgt attgataaag gtcacaactg gacggccaaa
    22681 tttctcagcc tgtttcatca accgcagtgc ctttcggtag ccttctggat gtggttggcc
    22741 aaaattccgt ttgaggttgt cttgcaaact cttgcctttt tggataccaa ccactgttac
    22801 agcttggtct ccaagccaac caataccacc aacaactgca ccatcatcac gaaaagaacg
    22861 gtcaccatgt aattggataa attcatcaaa aatgcctgtc gcaaagtcca aggttgtcaa
    22921 gcgactctgc tcacgcgctt ctctgactat ttttgcaata ttcatctagg actccctcca
    22981 tgcaatctga ctaggctagc aatcgtatct ggtaagtctc ttcttttgac aatagcatcc
    23041 acaaagccat gttctaatag gaattctgcc ttttggaaat cctcaggcaa gctttcacga
    23101 accgtatttt caatcacacg acgcccagca aaaccaacca agctctgtgg ttcagccaga
    23161 atgatatcgc cttccatagc gaaagaagct gtcacaccac cagtcgttgg atctgtcaaa
    23221 atggtcaggt aaaagagacc agcatttgaa tggcgtttaa ccgctgcaga gatcttagcc
    23281 atctgcatga gactcatgat tccttcctgc atacgggctc caccagaggc tgtgaatagg
    23341 acaactggca atttttcgac agtcgcatac tcaaacaaac gagtgatttt ttcacctaca
    23401 accgtaccca tagaagccat gataaaatta gaatccataa tcccaagagc cacagtctga
    23461 cctttaataa gagcagttcc tgtcacaacg gcttcatgca gacctgtttt ttcacgcata
    23521 gatgccagtt tcttttggta accagggaaa tgcaagggat ccttgctttc aatccctgta
    23581 aacaattctt tgaaggttcc catatcaatc gtcaaagcca agcgttcttg ggcagaaata
    23641 cgaaaggtat agctacagtg cggacagata cgttcacttc ccagatcctt ctgatagatg
    23701 gtatgcttac agcctggaca ctgggaaaat aattcatctg gaacctctgg cttagcttga
    23761 ggtttttccc taaccgaacg attgggattg attcgaatat acttatcttt tttactaaat
    23821 agagccattg attccccttt tcggtttaaa ctcttaaagt cattttattc tttttcttga
    23881 tatttaggta agaaggtttc catcaagaag gaagtatcat aatccccagc aatgacattg
    23941 cgatctgaaa tgagatcaag ctggaaatct gcattggtct gcactccttc aatctctaat
    24001 tcatagaggg cacgttgcat tttcatcaag gcgtcaaaac gattttcgcc gtgtactatg
    24061 attttggcaa tcatactatc ataataaggc ggaatggtat aacctggata aactgctgaa
    24121 tccacgcgca agccaactcc accacttggc agatagagat tagtaatctt acctggactt
    24181 ggagcaaagt taaaggctgg gttttctgca ttgatacgac actcgatggc atgaccgcgt
    24241 aggacaatat cttcttgctt aacagacaaa ggctgacctg ccgcaatgca aatctgttcc
    24301 ttaacgatat caacacctga aacaaactct gttactggat gttctacctg aacacgagta
    24361 ttcatctcca tgaaatagaa atttctactt gcttcatcaa gaagaaattc aatggttcct
    24421 gcattctcat agccaacaaa ctctgccgct cgaacagcag cagcacctat ttcatgacgc
    24481 agcgtttttc cgattgcaat cgagggactt tcttccaaaa ccttttggtt attcctttga
    24541 agagaacaat cccgttcacc caagtgaatc acatgtccat gctcatcacc taggatttga
    24601 acctcaatgt gccgagctgg atagataacc cgttctatgt acatggcacc attgccataa
    24661 ttggccttgg cctcactaga ggcagtttca aaggcagaaa cgaggtcatc tggtttttca
    24721 accttacgaa tccctttacc acctccacct gctgaagcct tgagcataac aggatagcca
    24781 attttttcag caacaatcaa agcttcttca gagttatgca cttctccatc tgaacctggt
    24841 ataacaggca cacctgcttt aatcatctga gcacgcgcat tgatcttatc ccccatcata
    24901 tccataacat gaccagatgg accgataaac ttgataccta cttcttcaca catggtcgca
    24961 aatttggaat tttcactgag aaatccaaaa ccagggtgaa tagcttctgc ctcagtcaag
    25021 actgcagctg atagaactgc attaatattg agataagact ctgttgcctt gccaggacca
    25081 atacaaactg cttcatctgc caaaagcgta tgaagagctt ccttatcagc agttgaataa
    25141 accgctaccg tcgcaatccc caattcacgt gccgcacgga taatacgaac cgcaatttca
    25201 ccacgattgg caattaaaat ttttcgaaac atggagaacc tccttagttc ccaattgcaa
    25261 aagtaaggat accactggct gcaagcttgc catccacttc agcctttgct tcaaccacag
    25321 ctatggtgcc acgacgtttt acaaaagtcg ctgtcataac caattggtcg cctggtacaa
    25381 cttgcttctt gaacttaacc ttgtccatac cagcgtaaaa gaccagtttt cctttatttt
    25441 caggttttga taactccaac acaccagcag tttgcgccaa ggcttccata atcagaacac
    25501 ctggcataac tgggtattga ggaaagtggc cgttaaagaa aggttcgtta atggtcacat
    25561 ttttgatagc aacaatggta tcctcgctca cttccaagac acggtccact agaagcatag
    25621 gataacggtg gggaagagct tctttgattc cttgaatatc gatcatttga tacgtaccaa
    25681 tcctttacca aactcaacca tttcttcgtt agagacgaga atttccgtta ccacaccatc
    25741 cttaggagct gggatttcat tcatgacttt catggcttcg ataattacca atgtttgacc
    25801 ttttttgaca ctatcaccaa ctgtaacgaa ggcaggttta tctggtccag cagccaagta
    25861 aaccactcca acaagtggac tctctacaag atttccctca gtagccacac ttgcttcagc
    25921 tggagctgga acttcttctg ctacagtctc tgctggagca gatgtaggag ctactggact
    25981 cggtgttgct agaacgggtg ctggagcgac ttgagttgca acttcaggca caggtcttgc
    26041 ttcattcttg ctaaactgca actcatccgt cccattttta taagaaaatt ctctcaaact
    26101 tgactggtca aattgagtca tcaagtcttt aatatcgttt aaattcatac ttatctattc
    26161 tcccaacgtt tgaaagcaag aactgcattg tggcctccaa aaccaaaagt atttgaaata
    26221 gcgtatggaa tttctttctc caaaccttgt ccataaacga cattagcttc gatataatct
    26281 gatacttcac ttgtcccagc tgtcattggt acaaagttat gacgcatagc ttcgatggtg
    26341 acgatagctt ctactgcacc cgcagccccc agcaaatgtc ctgtaaaaga cttggttgat
    26401 gatacaggta cttccttacc aagaacagct acgatagcac cactttctcc tttttcattg
    26461 gcaggagttg acgttccgtg agcattaaca taggctactt gctctggaga aatctcagct
    26521 tcttccaagg ctagtttgat ggccttgata gctccctgac cttctggatg tggagaagtc
    26581 atgtggtagg catcacaagt atttccgtaa ccaaccactt cagccaggat agtagctcca
    26641 cgtttttcag cgtgttcaag actttctaga accaacatcc ctgaaccttc acccataaca
    26701 aacccattgc gatccttatc aaatgggatc gaagcacgag ttggatcctc tgtagtagag
    26761 agagctgtta aggcttggaa accagcgatg gcaaaaggtg tgatagaagc ttctgttcct
    26821 cccaccaaca tcacatcttg gaaaccaaac ttaatggagc ggaaggcatc cccaatcgca
    26881 tcatttgatg aagagcaggc agtattgata gatttacaaa caccgtttgc accaaaacgc
    26941 atggctacat tcccagaagc catatttggt aaagcttttg gaagagtcat tggtttgaca
    27001 cgtttgggtc ctttttcatg aaggcgaagt acctgatctt caatttcctt gattccacca
    27061 ataccagatg caacgataac accaaaacga tccctattaa gagcctctac atcaagattg
    27121 gcatggttta cagcctcttg ggctgcatac aaggcatata aagaatagtt atcaaaacgg
    27181 ttggtatctt tttttacaaa gtatttatcg aacggaaaat cttggatttc tgccgcatta
    27241 tgcacatcaa agtcactatg atcaaatttt gtaatgccac caatgccgat tttcccagtt
    27301 gctaaactat tccaaaattc ttctggtgta tttccgattg gagatgttac tccataacct
    27361 gttaccacta ctcgatttag tttcattctt ttcacctcta gctttcgcta catacttaag
    27421 ccaccatcaa tggcaatcac ttgaccagtt agataatctt ggcctgctaa aaatactgtc
    27481 aaatctgcaa cctgctctgc ctgcccaaat tctttcatcg gaatctgagc tagtgtagct
    27541 tccttaatct tatctgataa gatagctgtc atatcagact caatcattcc tggagcaatc
    27601 acattgactc gtatattccg actagcgacc tcgcgtgcca cagacttggt aaagccaatc
    27661 aagccagcct tagaagcagc atagttagct tgaccaatat tccccatcaa accaacaaca
    27721 ctagacatat taatgatagc accttctctg gctttcatca tcggtttcaa gactgattgt
    27781 gtcatattaa aggcaccagt cagattgacc ttgagcactt tttcaaaatc tgcttctgtc
    27841 atcttgagca taagagtatc ttgggtaatc cctgcattgt tgaccaaaac atctactgaa
    27901 cccagttctg caatagcttg atcaatcata cgcttagcgt ctgcaaaatc tgatacatct
    27961 cctgaaatgg gaaccacctt gataccatag tttgaaaact cagcgagcaa ttcttctgag
    28021 attgccccac gactgtttaa gacaatgttg gctcctgctt gagcaaactt gtgggcgatg
    28081 gcaagaccaa ttccacgact cgaacctgta ataaagatat ttttatgttc tagtttcatt
    28141 tttttccttt caaaacttct acttatttta gtctattttt ttaaaagtgc tactaaactc
    28201 gcttgatctt ccacatgagc taagtgagta gtttgatcaa tttttttaac aaaacctgac
    28261 aagactttcc ccggtccaat ctcgataaag ttgcttatgc ctgcttcttg catgacccca
    28321 atactttcat agaaacgaac gggttccttg acctgacgcg tcaagagctg agcaatgtcc
    28381 tctttttgca tcacagcagc ttctgtattg ccgactaggg gacaagtaaa atctgaaaaa
    28441 cttacctgag ccagagtttc agctagtttc tggctagcag gttcaaggag agaggtgtga
    28501 aagggacctg acaccttaag aggaatcaag cgtttggcac ctgcttcttg caaaagttca
    28561 accgctcgat caactgcaac cacttctcca gcaatgacga tttgtgcagg tgtgttatag
    28621 ttggctggag taaccactcc aagttcagaa gctttttgac aggcttcttc aatgacctct
    28681 actggcgtat tgagaactgc taccatcttg ccagagtcag caggagccgc ttcttccata
    28741 taggctccac gcttagctac caaggcaacc gcatcttcaa aatccaaggc gccgcttgcc
    28801 accaaggcag agtattctcc aagagacaaa ccagcaacca tatcaggctg atagcccttt
    28861 tcttgcaata aacggtagat agcaaccgaa gtcgctagaa tggctggttg cgtatagcgg
    28921 gtctgattga gtttgtcttc ttccgtatcg atgagataac gcaaatcata acctagcacc
    28981 tgactcgctc gatcaatcgt ttctttaaca atcggatact gatcatagaa atcccgtccc
    29041 atccctagat actgggcacc ttgaccagca aataaaaagg ctgttttagt catttcttac
    29101 aactcctgtc cagcgagagg cttcttcttg aattttctta gcggctccgt aatacaaatc
    29161 ttttaggatt tcttcagctg tttcttcttt agaaacaagc cctgcgattt gacctgccat
    29221 gacagagcca ccctccacat caccgtgaac aactgctttg gctagggcac ctgctcccat
    29281 ttgttcaaag atttctaaat caggatcttc ctgcttaaag gcatcttttt cagccagttc
    29341 aaaatctcta gtcaactgat ttttaatagc acgaacagca tgaccaaagt gctgagctga
    29401 aatcgtagtg tcaatatccc ttgcttttaa aattttctcc ttgtagtttg gatgggcatt
    29461 cgactctttt gcaactacaa accgtgtccc cacctgtaca gcctctgcac ctagcataaa
    29521 gccagccgca gcaccttcac catccgcaat tcctcctgca gcaataacag gaatagatac
    29581 agctgtggct acctgtcgca ccaaggtcat ggttgttaat ttaccgatat gccccccagc
    29641 ttccattcct tctgcaataa cagcgtctgc accgattttt tccatgcgtt tagctaaagc
    29701 gacactagga acgacaggaa taacgattat cccagcttca tggaaacgtt ccatatactt
    29761 gcttggattt cctgctcctg ttgtgacaac tttaacacct tcttcaataa cgagatccac
    29821 gatatcttcc acaaagggag ataagagcat gatgttgacc ccaaagggtt tatcagtcaa
    29881 tgatttgatt ttatcaatat tggccttgac aacttctttc ggggcatttc ccccaccgat
    29941 aattcctaat cctccagcct tggaaacagc ccctgccaaa tcaccatcag caacccaggc
    30001 catccctcct tggaaaatag gatagtcaat cttcaataat tctgtaatac gcgttttcat
    30061 agtgcctcca accttccttg cttacgtaat agttcgattt caccataatt tgacagtcaa
    30121 actattacct aaacaagagg gagtgggttt ctccctactc cttctactaa tattctgctt
    30181 attttgcttg ctcttcaacg taagcaacca agtcaccaac tgttttcaag tcattttctg
    30241 cttcgatttg gatatcaaaa gcatcttcga tttctgagat tacttggaac aagtccaatg
    30301 aatctgcgtc caaatcatca aaagttgatt caagtgttac ttctgatgcg tcttttccaa
    30361 gttcttcaac gataatttct tgtacttttt caaatactgc catgatagga ctcctttaaa
    30421 ataaatagtt tttttataac aatgtgttca ccacatgatt acctaaattg taagaatgag
    30481 cgtgccccag gtcaaacctc caccgaagcc tgatagaaga acagtctggc taccatctaa
    30541 agggatgaga ccttgttcta cacactctga aagtaaaatc gggatactgg ctgcactggt
    30601 attgccatat tccatcatat tggctggaag tttggctcgg tcaacaccaa tttttctagc
    30661 catcttatcc aaaatacggt cattggcttg atgaagtagc agataatcca agtctgtcac
    30721 ctctatagga gattcatcaa tagtctgctt gatagacttg gctacatctc gaatggcaaa
    30781 atcaaagact gtgcgtccat ccatcttcaa aaacgaatct gcactttctt gatctgaaaa
    30841 tggagaatgc aaacctgaat gcccataagt taaacactcg ctgcgacttc catcgctatt
    30901 gagactctca gctaagaaat gctcttgctc gctagcttct aacaagacac caccagcacc
    30961 atctccaaac aacacagctg ttgatcgatc cgaccaatcg actgccttag agagggtttc
    31021 actaccaatc accaagcctt tttgaaagcg accagaagcg ataaactttt cagcagttga
    31081 aagagcaaat acaaatccac tgcaagccgc ggttaagtca aaagcaaagg ctttattagc
    31141 accaatatta gcttgaacac gagcagctgt agagggcatc atcgaatctg gagtaatggt
    31201 agctaggatg ataaaatcca gttcttctcc tgttattcca gcttttgcca tcagtttctt
    31261 agcaacctct gtagccaaat cactggtaga ttctgttctt gaaatatgcc tttgtcgtat
    31321 tcccgttcga cttgaaatcc actcatcatt ggtatccata atctgagcca agtcgtgatt
    31381 tgtaaccact tgctctggca cataatgagc aacctgactt atttttgcaa aagccattat
    31441 ttcaaatcct ccaaaaattg gtaaagatta gtcaagcctt tacccatgac agcaatttct
    31501 tcctcgctca tgccatcaat aattttttct accatggcct tgtggaagcg tttatgcagt
    31561 ctatgaatca agcgaccctt ctttgtcaaa tgcagatgca ccacacgacg atcctgttct
    31621 gaccgaactc gctcaatgta gcccttacgc tctaaattgt tcaaacttgt cgtaacagtt
    31681 ccaagagtta ccattaactc ttttgacact tgacttggag tcacatctgg agccttaccg
    31741 atgacatcaa ttgtatgcat ttctttgatg gagatatcct taaaacgact acctctcaag
    31801 ttcacttcct caattacaag gacattgtta aatatagatg ttaaatattc attgattcgt
    31861 tggtagtcca tttttcatat ccctccttct tcaaaaaact tttacaatca aaacatttga
    31921 cagaaagaat tatatcaaaa ttcaaagaat gatgcaagta ttttttattt tcctgtaaat
    31981 ttaggtcttc ttctctccga atgagctcgc actccttctt tgaaatcctc tgtttgagct
    32041 aaggatttct gtaggttcag ttctaaagta gcgtaaccct gccagtcttt aaattggctc
    32101 tcccaaacca acttcttaat ggcagcatag gaattacttg aagcacgtct taatttttta
    32161 agaagctgtt ctctcgtctt ttcaagtttt tcagcttcag aaacgcggta aaccaacccc
    32221 cattctaggg ctttttctgc cgttaaagcc tctcctgtca tagctaattg agcagcacgc
    32281 gtcacaccga tactgcggct caagagatga attccccctg catctggagc caaaccaaca
    32341 ccaacaaagg cttggataaa cttagcctta tccgtcgcca aacagaaatc tgccgcaaca
    32401 gccatattcg ctgcggcacc tgcaacagca ccgtcaactt ccattaaaac aggtttggct
    32461 atttgcttga ttttataaga aatcgtattg accaactctg cgatttttgt caatgatgga
    32521 atatcatcct catccactgc ccgcttcatc tctaccaaat ctcccccaac tgagaagacc
    32581 tttccattgg cattgattaa gataaaatgc acagctggat tttcttctgc caaagtcaga
    32641 gcttctaaaa tctcctcaca catgggaata tgaaaaccat ttgcgacctc aggacggttc
    32701 aaggtaagga ttgccaagtc ctcttcaagc tgataaataa tgtgttccat cagggctcct
    32761 ttttatttta taagcagttt atactcttcg aaaatctctt caaaccgcgt cagcttccat
    32821 ctgcaacctc aaaacagtat tttgagctga cttcgtcagt cttatttaca acctcaaagc
    32881 agtgctttga gcagtctgcg gctagcttcc tagtttgctc tttgatttca ttgagtgtta
    32941 aattatttca ctgtcaaagt atatcttttt taggagcaga aagcaagaaa aaatcgactg
    33001 aaagtgtctc agccgataag aaaggctcgc tttaggctag aaagcatctt attttcttca
    33061 ttgaaaaaag cctttctttc tgctataatc gtataaaata cttactttag gagttcttat
    33121 gaaagttgtt aaatttggag gtagttctct tgcctctgct agtcaattag aaaaagtttt
    33181 aaacatcgtc aaaagcgatt cagagcgtcg ttttgtagtc gtttctgcgc ctggtaaacg
    33241 caatgctgaa gatactaagg ttacggatgc cctgattaaa tactaccgcg actatgttgc
    33301 gggtaacgat attagcaaga gccaaagctg gattatcgac cgctatgctg ctatggttag
    33361 tgaattggga ctaaaaccag ctgtgctaga aaaaatttct aaaagcattc acgccttggc
    33421 cactcttcct attgaagaaa atgaatttct ctacgatact ttcctagcag ccggtgaaaa
    33481 taacaatgcc aaattgattg ctgcctactt taaccaaaat ggtatcgatg cacgctatat
    33541 gcaccctaga gaagctggga ttgtggtcac aagtgaacct ggtcacgctc gcatcattcc
    33601 atcaagttat gacaagattg aagaattgac aaacaccaat gaagtccttg tcattcctgg
    33661 tttctttggt gtcactaagg aaaatcaaat ctgtactttc tcacgtggag gttctgatat
    33721 tacaggttct atcattgctg ctggtgtcaa agccgacctc tatgaaaact ttacggacgt
    33781 tgatggtatc tttgcagccc accctggtat tatccaccaa ccacactcga ttcctgagtt
    33841 gacctaccgt gaaatgcgcg agttggccta tgcaggcttc tcagtccttc atgacgaggc
    33901 tcttcttcct gcctaccgtg gaaaaattcc tctggttatc aagaatacca acaaccctga
    33961 ccatccaggt actcgtatcg ttctaaaaca cagtaatgat gaatttccag ttgtgggaat
    34021 tgctggtgac tcaggctttg tcagcattaa catgtcgaaa tacctcatga accgtgaggt
    34081 tggatttggc cgcaaggttc tgcaaatcct ggaagaactt aacatcggtt gggaacatat
    34141 gccaacaggt atcgacgatc tttctatcat tctccgttct cgccaactaa ctcccatcaa
    34201 ggaagaagaa atcctgcgtc agttggttca aaaggctaaa gtagatcatg cagaaatcga
    34261 acacgacctt tctatcatta tgattgttgg tgaaaaaatg aagagccaca tcggagtgac
    34321 tgctactgcg acacgcgctc tatctgaaaa caaaatcaac atccagatga tgtctcaagg
    34381 ttctagtgaa gtttctatta tgtttgttgt caataaagac caagagaaag cagcaattaa
    34441 agccctctac aatgcctttt ttggagaaag taaggaagac taagcatggc ccaatcactt
    34501 aacaaaacag tactcctcag tacgacaggc acttcctacc tctctatcgc tgggaaagtt
    34561 gggaaattcc ttgtcggaga tcaggctttg gaattttacc cagatgtcaa tgtcgaacaa
    34621 tttatccaga ttccttggag ccatatcaat cagattggag ccaatgtcac tggacgcaaa
    34681 atcagccgcc acttcgaagt ctttacagac aaaggaaaat tcctctttgc ttcaaaagac
    34741 tcaggtgcca ttctcaaaat tgcacgcgaa aaactaggca atgacaaggt cgtcaaactt
    34801 ccgaccctga ttcagacaat tagccaaaaa tttaaaaatc tatttgcaaa aaagtagaaa
    34861 ctttagtata atcatagcaa cggataagta ggttatcttc ttctttcaga aagtctgcgg
    34921 gtactgcgag cagatagggg ggataggtga aattctaccg ttagtaacaa ttacatacac
    34981 aatcaagtgc acacctgtga agttggatgg aaccgtggcc ctgccactcc aacgttttgt
    35041 caggcgtgct tttttcataa aggagttctt atgttagata tcaaacgtat tcgtacagat
    35101 tttgaagctg tcgcagaaaa attagctaca cgtggtgtag aggctgctgt cttgaatgaa
    35161 atgaaagaaa tcgatgctaa acgtcgtaac atcttggtca aggttgaaac tctcaaagca
    35221 gaacgtaaca cagtttctgc tgagattgcc caagctaagc gcaacaagga aaatacagat
    35281 gacaagattg ctgccatgca aaatctatct gctgaggtta aagccttgga tgctgaattg
    35341 gcagaaatcg atgctaaatt gacagaattt acaacgactc ttccaaatat cccagctgac
    35401 agcgttcctg ttgggactga cgaagacgac aatgtggaag ttcgccgttg gggtactcca
    35461 cgcgagtttg acttcgaacc taaagctcac tgggatctcg gtgaagacct tggtatcctt
    35521 gactgggaac gcggtggtaa ggtaacaggc gctcgcttcc tcttctataa aggcctcggt
    35581 gctcgtttgg aacgtgctat ctacaacttt atgttggatg aacatggaaa agaaggctat
    35641 actgaagtca tcacacctta catagtcaac catgattcta tgtttggtac tggtcagtat
    35701 ccaaaattta aggaagatac ttttgaactc agcgatacca actttgtctt gattccaact
    35761 gctgaagttc ctctgacaaa ctactaccgt gatgaaatct tagacggcaa agatcttcca
    35821 atctacttca ctgccatgag tccgtcattc cgttctgagg ctggttctgc cggtcgtgat
    35881 acgcgtggct tgatccgttt gcaccaattc cacaaggttg aaatggtcaa atttgccaaa
    35941 ccagaagaat cttacgaaga attggaaaaa atgacagcca acgctgaaaa cattcttcaa
    36001 aaactcaacc ttccataccg tgtcgttgct ctctctactg gagatatggg cttctcagct
    36061 gcgaagactt acgacttgga agtgtggatt ccagcacaaa acaattaccg tgaaatctca
    36121 agctgttcaa acacagaaga tttccaagcc cgtcgtgccc aaatccgtta ccgtgatgaa
    36181 gcagatggca aggtgaaact ccttcatacc ttgaacggtt ctggacttgc agttggacgt
    36241 acagtggctg caattcttga aaattaccaa aatgaagatg gttctgtgac catcccagaa
    36301 gcacttcgtc catacatggg tggagctgaa gtcatcaaac cataaaaaat aaggtttagc
    36361 tatttctagc tagacctttt ttcgtaacca aatcagataa gcacctagta caaagaataa
    36421 aatagttagg catataatgg tttcagccaa taccaggtaa tccagaaatg gaagtttcaa
    36481 aattccctga gccatcttga gcgaggtcgc tgtgataatg gttgggaagg tgagggctga
    36541 gaaggctggt tgaaaacctt gttttaaaat gttgggcaga cgagttaaaa caaagaaaaa
    36601 gaaggattga gaagccaaaa tcatgacaat caagacccaa gtcggcaggc tggttcctcc
    36661 tactcgaact agagaagcca agagtagaga gaaaggagca cagtagattc cttcttgtcc
    36721 aagcaaggct agtgggagtg gatgtttctt taaatcgcta taaataaggg gatagagata
    36781 gaaggtcaag agaaaaccaa aactcaaggt cgcataggca atttcgataa tacctaccag
    36841 aggataggtc aaggcagcca ctgctatccc cacatagaga accgtccaac ttggagtggc
    36901 atgaaccctc cgccctgaac aagcaaactt gatggtaaaa ccagcaatca aggtcaaatc
    36961 caagagaaat gaaaaccacc aaatcccttg tgctaccaaa ggaagataag agaatacgcg
    37021 aaagacatag gtcgataaaa tcatcccagc cataggaaag gttgccattc ctgacaaaag
    37081 agggggcttg gtcaattctt gcttggtttc tttccaatta aagagatgca gaattagaaa
    37141 gtaaatccat aaaaccaaac cagtcagact aaaaagatgg gatagaaccg gcaacgtatc
    37201 taaaataaga tttccagctc ctgccaaacc tagcaaacaa cctgaaaata ctaaggggag
    37261 ttttttcatc ctaacctcca ataatcatgt tagtttcagt ataacataaa agcgcttaaa
    37321 tgaggattta aaaaaacgaa gtccgcttat ttcagacttc attttactca gatatgaatt
    37381 aggcataagg ttgcaattct ggattaattg gtgtattagc taagttgttg gcatagttac
    37441 agaggattgc taggctgaca ccaaaaacca catccaaggc attttgttga gtgtagccag
    37501 cttctaaaaa ctcagacaag gcttcatctc ctacacgacc cttggtattg ataactgcca
    37561 aggtaaactt agctagggta tccaatttag gatctgtttc aattggagta cgattgcgaa
    37621 gagcttgaat caagtcatca ttcatctgga tttgtttgat ggaaaaggct gtgtgacctg
    37681 cgacacagaa ggcacaacca ttggtcacgg ctgccgtgat ttgcaccact tcacgctcaa
    37741 cgggtgtcag gctgttgcga cggtggatag atgagacaat ttggtaggct tctaaaacag
    37801 tcggggcatt ggccaagaga ccgattaggt tgggaatata gccattgttg tctttttcta
    37861 ctgtttcaag aatttctttc acttctgctg gtgctgactc tactgtatgg atagtaaatg
    37921 ttgtcataag atacctcttt tcttattatt gacactaata ttattggaaa atcttataaa
    37981 atcctgattc ctaagtttat ctaagataaa gctttattct ctcataagat tttcgttgtt
    38041 atattagttt atcacacttc caatcacttg cataatatat attatatatc aggctgataa
    38101 aaattattta taggcaaaaa aatcacacga gctgtgtgat tccattattt gtcaaaatac
    38161 tttttagttt cagcaataac gactggcgac aagaccaaga gggcaatcaa gtttggcaga
    38221 gccatcaagg cgttaacgat atctgcgata atccagacca tatccaactc gataaatcct
    38281 cctaacaaga ccatgagcac aaaaaacaca cggtagagcc agataaagcg agccccaaag
    38341 aggaactcaa aacagcgttc tccgtaatag ttccaaccta gaatcgttgt aaaggtaaaa
    38401 agtacaagga agatggtcaa gagggcaggc ccaaagtgtg aaaagactgt tgagaaagct
    38461 gactgagtca aggcaacccc attcaagtca ccactccaaa ctccagttac caagatggtc
    38521 aaaccagtta gagtacaaat gatgagggta tcaataaagg ttcctgtcat ggaaatcaaa
    38581 ccttgctcta ctggttcatt tgtcttggca gctgcagctg caataggagc agaacccaga
    38641 ccagattcgt ttgagaacac accacgcgcc acaccatttt gaatagccat ccgaacgcta
    38701 gcaccagcaa atccacctac cgcagcaagg ggactaaaag ctgaggtaaa gactaaagcg
    38761 attgtgccag ggatttttcc gatattaaag aaaataactg taagagttcc taagatataa
    38821 atgagggcca taaaaggaac aacagtagtt gaaaccttag aaatagactt gagtccacca
    38881 aagactgcaa tcgctacaaa gacagacaag acgagagctg tgatggctgg cgaaatcgtc
    38941 gttgtatttt ggatagattc tgtaatcgag ttgacttggg tgaaggttcc gattcccaag
    39001 agagcaacca atactcctgc tactgcaaac aaaacagcaa gtggtcgcca cttttctccc
    39061 atccctagaa ggatatagtg catgggacct cccgctactg caccatggtc gtccttggtg
    39121 cggtatttga tggccaagag tccttccgca tacttggtag ccattccaaa gaaagccgcc
    39181 atccacatcc aaaatagagc tcctggtcca ccaaccttga tagccgtcgc aactcctatg
    39241 atatttcctg ttccaacagt tgatgccaag gctgtacaca gagctgcaaa actggataca
    39301 tcaccatgtc ccttatcctg gataaaaata agctgaaagg ccttgggcag acgcaaaacc
    39361 tgcaagagtc ctagccgcat ggttaggtaa atccctgttc cgaccaataa aatcaagagg
    39421 ggcggtcccc aagcaaaagc atcgattgat ttaagcaatt ctaacatttt cttctcctat
    39481 cgtttcaacc ccaaaagaaa gagcacatgc aagatacatg tactctggaa tgcttagata
    39541 aatgctaaaa agcggtctat cctagctctg tccttttacc tgagagtttg agcagttgcc
    39601 tgccttgccc cttcggtgcc tttacggtct ctccagagtt ccgtccattt acagtcatgg
    39661 aaaatcaaac gattccccac ttctattaaa cttcattcgg tgttggtatt taattgattc
    39721 taattttaca aaaaatgttg gcttttgtca atgtgtttat tagtaaaaat tagttcaaca
    39781 gtttttactt tataaagtcc agaatactgc tatcctttaa aagtgacaat agtcgcacca
    39841 ctgcctccag cattttgtgg ggcatagccg aaactcttga catgtttgtt tctttgcaag
    39901 tatttggtaa ctccttcacg gatgactcct gttccgatac catggatgat atcaacttga
    39961 gccatattgt taagcaaggc ttggtcgatg aaggtatcta gctcattcat ggcttcttca
    40021 tagcgcttgc ctcgaagatc cagtctagct tgaggtcctc gcccagaagt tcgtttcaca
    40081 acattgacct gtttcttctt gactggtttt tcttgctggg cttgaacaag atcaaactct
    40141 ttctcttcca aggtcatctt aatcaagcca atttgggctt cccagcggcc gtccttgagt
    40201 tgactggtca aggtaccacg ctgaccataa ctgagaacca cgatatcatc tcccaccttt
    40261 ggagctcgtt ttttcttggc cttttgaagg accttgtttt tagacaagtc cactttttca
    40321 ggagccaatt ttttcaactt ggccttggct tcaatgattt cgtggggctt gagttgggat
    40381 ttactgtgga gatttttgag aatctggtca ctttcactta gggccatatc cacaatctca
    40441 gcagcctgtt cacgcgcctt gttaagctcg gtttcctttt cacgattaag ctcgttgtag
    40501 agttttttta gcgcacggtt catcttgaga ttttcttgct ccacctcacg gatattgtcc
    40561 aagcgtttgc ggctttccag cgtctgctct tctaattgct caatgatacg attgacgtca
    40621 ttgtcctgat cgatctgctg actggcatct cctacgataa cttcagatag gcctagacgt
    40681 ttggcaattt caaaggcatt acttcggcca ggaacaccct gcataaagcg ataggtcggg
    40741 cgaagagttg cagtatcaaa ctccatactg gcattttgca caaaggctgt ctcaataccg
    40801 taggccttga gttctggata gtgggtcgtc gccatggtct tgatttgacg caggcgaagg
    40861 tcctccagaa tagccatggc aagggctgct ccctcttggg gatcagtacc agcccccaac
    40921 tcatccaaaa gtaagagtga atgttggttg accttgccaa gaatatccac gatattggtc
    40981 atatggctag agaaggtaga caagctctgc tcaatagact gctcatctcc aatatcagca
    41041 aagatttctt caaaaatacc aacacgactt cccttgtctg ctaaaatcgg caatcctgac
    41101 tgggccatga cctgtgtcaa gcccagagtt ttaagcatga tggtcttccc acctgtattg
    41161 ggacctgtaa tgacaatagc tgttaaatct tgaccaaaat agacatcatt tgcgacggca
    41221 tttttgacca aaggatggca gacatggagc agttgaatct cttgattttc tgacagctga
    41281 ggcacgactg cttgtctttc ttgaataaat cgaaccttgg cacgaatcaa gtccagatga
    41341 ccgataatcc aagcgtcatt agcaatctca gccgcatgag ggcggacacg ctcagaaatt
    41401 tcttggagaa tgcgaagcat ttcatagcgc tcatctgctc gcagactagc aatttcttcg
    41461 ctcagtttga ctacctcacg tggttcgata tagacggtgt ttccactagc agaaatatca
    41521 tgaacgacac ctgcaatctt attgcggtag gtgtttttga ctggtaaaac ctgacggcca
    41581 tttctgctag caacaattcc ttccgtcaac atctgccctt tttgcttgag caagtcttgt
    41641 aaaacatcgc gtacctgact ctcgctatca tgtatttttc gacggattcg cgccaattct
    41701 tcactggcaa aattttcaat gaaacccgca tcattaaagg cctgaagatt tccttgtaat
    41761 tgcggaaaat catgtaattt ctcaaaccaa agggctaatt cttccaagct gacattttcc
    41821 agattggcgt aaaaactttg aagttctcgg ctggcaagaa gcacgcgttt caagagtagg
    41881 aactcctcga tattgagatc cgctcccatc tccaacctct tgcagactcc tgcaatttcc
    41941 ttagttgaga gaatagtaaa atgcggttgc tcgacgaaaa gagcctgcat ttctttcatc
    42001 tcagcaaaag cctgtttgat tttatctgct ttggcagtcg gagccagctg tctcaattgc
    42061 tccaagccct gctcggtcaa caaatgaggc tcaaacaagg ccttgacctt atcgaactct
    42121 aatgtttcta atattttctt attcatcttt atttccttgt ctttgaatgt ctaggtgtgg
    42181 tagcttttta cattctgata gattcgagtc aaatctgtaa acaaagggct aggaaaactc
    42241 ctgccctttt tatccgatta aatttgtcac ccagatttgt ttgagccaac tggttgttat
    42301 cggtatgctc tggatgatgt gttttgcgac gatactcttt tcaagaggat tttgtataac
    42361 tgccataggg atggtcgcca agattgtcaa ggccatttgc aagacaaata aggtcaccaa
    42421 catggacaag atacctgctg aaacttggaa caacttacca cccagttttt tgctaggaat
    42481 caagtgtaag agaagaccaa gcaaacgacc aatgctatag acaatcccaa atacaagcaa
    42541 gtagccgata cctgcataaa agaccttatc cagctgaaag agttgatccg atgggaaaaa
    42601 gaaagtcccc tgaccttcct gcgaatttgc ataagggagc aataaatgga attgctctcc
    42661 aagcccctta taaaactggc cagccataaa agccgatgcc atggctgaaa tcaggtaata
    42721 aacctgtaag agcaggcctc tccgatagcc gatataaaat ccccaaacca agaccaatag
    42781 aagaaggaat gaaatcataa ggaatcctca atcttgctct gttcttgctt acaagtcaca
    42841 agcttgtgac ggagttcttc tagctcttgc tccttatcgt caaattcaat ctcacggctg
    42901 agctgagttg ataaacagtt gactgccaac aaaagagcga ttgtttcatc atctgcgcta
    42961 ggcatttgtt ctttaattgc ttggtatttt tctgtcgcaa ccttagcgat ttcctccata
    43021 aaaaggttat catgttcgct tgtcaaggtt aacgtttttt tcccgaatgt aaatttgaat
    43081 cgatttagat ttgccataaa attcacctca cgatattata ccaaaattcg ctaattttgt
    43141 cagtttttac aaattttact gcttttgtgg tacaatagaa actatggcaa gtatcacact
    43201 cacaccaagc gaaaaggata ttcaggcttt tcttgaacac tatcaaacca gtctggctcc
    43261 tagcaagaat ccctatatcc gctacttttt gaaactacct caagcaacgg tttctatcta
    43321 tacttctgga aaaatcttgc ttcagggtga aggggctgaa aaatacgcca gtttctttgg
    43381 ctatcaagct gtagagcaaa ccagcggaca aaatcttcct ttaattggga cagatgaggt
    43441 gggaaatggt tcctactttg gtgggcttgc agttgtggct gcctttgtca cacctgacca
    43501 gcacgacttt ttacgaaaac tcggtgtggg ggattctaag actctgaccg accaaaagat
    43561 ccgtcagatt gctcctattc tcaaggaaaa aattcagcac caggcactcc ttctctcacc
    43621 cagcaagtac aacgaggtca tcggagaccg ctacaatgct gtttcggtta aggttgccct
    43681 ccataatcag gctatctatc tcctccttca aaaaggtgtt cagcctgaga aaattgtgat
    43741 tgatgccttt accagtgcta aaaattatga caagtacttg gcacaagagg ccaatcgttt
    43801 cagcaatcct atcagcttag aagaaaaggc tgagggcaaa tacttggctg tcgcagtttc
    43861 ttctgtcatt gcgcgtgatc tctttctgga aaatcttgaa aatctgggac gagaactggg
    43921 ttatcagctc ccaagtggag ctggaacagc ttctgacaag gtggctagcc agattttgca
    43981 agcctatggt atgcagggac tcagcttctg cgccaaattg cactttaaaa acactgaaaa
    44041 agcgaaaaaa cgcttagaaa ggtaagttat gaattcattt aaaaatttct taaaagagtg
    44101 gggattgttc ctcctaattc tgtcattact agctttaagt cgtatctttt tttggagcaa
    44161 tgttcgcgta gaaggacatt ccatggatcc gaccctagcg gatggcgaaa ttctcttcgt
    44221 tgtaaaacac cttcctattg accgttttga tatcgtggtg gcccatgagg aagatggcaa
    44281 taaggacatc gtcaagcgcg tgattggaat gcctggcgac accattcgtt acgaaaatga
    44341 taaactctac atcaatgaca aagaaacgga cgagccttat ctagcagact atatcaaacg
    44401 cttcaaggat gacaaactcc aaagcactta ctcaggcaag ggctttgaag gaaataaagg
    44461 aactttcttt agaagtatcg ctcaaaaagc ccaagccttc acagttgatg tcaactacaa
    44521 caccaacttt agctttactg ttccagaagg agaatacctt ctcctcggag atgaccgctt
    44581 ggtttcgagc gacagccgcc acgtaggtac cttcaaagca aaagatatca caggggaagc
    44641 taaattccgc ttctggccaa tcacccgtat cggaacattt taagaaacct aagaggccga
    44701 gaatcaccaa tctcagcctc ttcttctatc gtgagaagtg attgtttatt gacaaatact
    44761 caatgaaaat caaagaacaa actaggaagc tagccgtagg ctgcttaaaa cagtgttttg
    44821 aggttgcaga tggaagctga cgtggtttga agatattttc gaagagtata aaataaaaac
    44881 aatcccaact ctcacctatt catgcaaagg aattttatgg aagtttattt ttcaggaacc
    44941 attgaacgga ttatttttga aaatcccagc aatttttatc gcatcctcct cctagaaatc
    45001 gacgatacgg acgcagagga ttttgatgac tttgaaatca ttgtcacagg aaccatggct
    45061 gatgtaattg agggcgaaga ctatactttt tgggggcaaa ttgtccagca ctccaagtat
    45121 ggagaacaac tgcaaatcag tcgttatgat cgcgcaaaac caactagtaa gggcttggtc
    45181 aagtactttt caagtagcca tttcaagggg attggtctca agacagctca gaaaatcgtg
    45241 gatacctatg gcgaaaatac cattgacgaa attttgcaac acccagaaaa gttagaaggc
    45301 atcgcaggac tctctgccaa aaatcgcgag gctttcgtct ccactctccg tctcaactac
    45361 ggtacggaga tggttttggc caaactagcc aactacggca ttcccaacaa actagccttt
    45421 cagattcaag acttttacaa ggaagaaacc cttgatgtgg ttgaaaatta tccctaccaa
    45481 ctggttgagg atatcaaggg tttgggcttt accattgctg accaactagc tgaggaacta
    45541 ggcatcgaaa gtcaggctcc tgaacgcttc cgcgccggtc tagttcacag tctttttcag
    45601 gcctgtatgg aaacaggaga cacctatgtt gaagcacggg atttgctgga acaaaccctt
    45661 actctccttg agtcttcccg tcccgtggaa ctagacccca gccaagttgc ccaagagctc
    45721 tcctacctga tcgaagaaga caaggttcag cagattgata ccaagatctt tgacaacagc
    45781 ctctttttcg ctgaggaagg catccgcagt cacttggttc gtatccttga aaaaggaaaa
    45841 cagaagagtc atgatttaga aaccattcaa aaacatatca ctactatcga ggaagagctg
    45901 ggaattgagt atgataacat tcaaaaacag gctatctgtg acgctatcca gaacaaggtc
    45961 tttatcctga caggtgggcc tggtactggt aagacaactg ttatcaatgg aatcatcgct
    46021 gtttatgccc ttttagaagg acttgacttc aggaagaaaa acaatctgcc cattcttctt
    46081 gctgctccaa ctggacgagc cgctcgccgc atgaatgaat tgacaggttt gcctagcgcg
    46141 accatacacc gccacttggg aatgacaggt gacgatgata ccagtcatct ggaagattac
    46201 ctagatgctg actttatcat cgtggatgaa ttctctatgg tggatacttg gctggccaat
    46261 caactcttct ccaatatctc ttctaacagt aagatcctca tcgtgggtga cagtgatcag
    46321 ctaccttctg tcagtcctgg acaggttcta gcggatctgc ttcatattcc tttgattcct
    46381 cagactcgct tggaaaaaat ttatcgacaa agcgaagaat caaccatcgt caccctagct
    46441 agtcagattc gacagggcat cttgccagct gatttcaccc aaaaaaaagc tgaccgttcc
    46501 tactttgaaa ttgctagtgg ccatattcct gccactattg aaaaaatctt aggtgccgcc
    46561 ctcagaaata gtattcctgc ccgtgatatc caagttctgg ctcctatgta ccgagggacg
    46621 gcagggattg atgctatcaa tcagctcatg caagacctgc tcaatccccc acaaaaagat
    46681 caactcagtt ttgaagctcc tcagtgccac tatcgtaagg gagacaaggt cattcatttg
    46741 gttaacgatg ctgaaatcaa tgtctttaat ggggatttag gagctatcac agacctgatt
    46801 cctggtaaat acaccgaatc gaaacaagac gagattgtca ttgattttga tggtaatgaa
    46861 gtctcttacc cccgtaacga atggtacaag attcgcttgg cctatgccat gagtattcat
    46921 aagtctcagg gaagtgagtt cccagttgtc atcctaccga ttactagtgc tagccgacgt
    46981 atgctggagc gaaatctcat ctacacagcc attacacgtg ccaaaagcaa actcatctta
    47041 ctaggcgaat tacaagcctt cgactatgct acccaacaca tcggaactgc ccgaaaaacc
    47101 tatctgattg aacgcttcag tgatctactg gagaatgttg aagaaaagca acaagctgtc
    47161 tctgaaacag tcacatcaag tgcctctgaa caatcctaca tcctaaccga agaaaactgg
    47221 gacagcattc cagccatgat tgggattaca gacacagacc tcaaagagat ttttggaaaa
    47281 tagtgcatca gaaaaaccac caattcaggt gggttttctg tttattaaga ttattttact
    47341 gttgctgtgc ttgtgatcaa ttcaacagct tttttgatag tgatatcatg tttcaacatg
    47401 tcagctgaaa gcaagttttg aacttgtgca acttccatgt tgtagtctgc tgccaattgc
    47461 tcaacttctt tttggatttc ttcttctgaa gcatcaaatc cttcagcttt ggcaactgct
    47521 tcgataacaa ggttagtctt agtacgtgac tcagcttctg cttggtattg gttgtgaagg
    47581 tcttcttgag tagttccagt gatttggaag tacatgtcag ggttgatccc ttgacgttgc
    47641 aaattcccaa ggaattcatt tactgaacgg tgaacttctt catggatcat ttcttctgga
    47701 agttctacga tttcagcatt ttctacagct gtatcaattg ctgcaccttc aactgcatct
    47761 ttgtaagctt cttctttagc agcagccaat tctttgcggt atttttcttt caagtcagca
    47821 agtgtttcaa cttcttcatc aatgtctttt gcaagttcat cgtcaagagc tggaacttct
    47881 ttagctttta cttcgtggat agttgtcacg aatttagctt ctttacctgc aaggtcttct
    47941 gcttggtagt cttctgggaa tgttacgata acatcaacgg tttcgccagc tgagtgacct
    48001 accaattggt cttcgaaacc agggatgaat tgacctgaac caagtccaag tgagaagttt
    48061 tcaccttttc caccgtcaaa ttcaacaccg tcgatagaac caacgaagtc gatcacaaca
    48121 gtgtcgccgt tttcagcagc agcttccttg ataaccaatt cagccaggtt gttgcgttcg
    48181 cgttcgatac gctcttcgac atcagcgtca gttacttctt tttctacatc aactgatact
    48241 tcaaggtttt tgtagtcacc caattttact tcaggttttg taacgacttc agcagtgata
    48301 acccagtctt gacctttttc cattgaagtt acgtcaattt ttggttgggc aaccacttca
    48361 agaccagctt cttttacagc tgcttcataa gcgtttggca aaagagcgtt cataacgtct
    48421 tggtaaagtg attcttcacc aaatttttgg tcgaagatag gacgtggaag gtgaccttta
    48481 cggaaacctg gaacattaag agatttcttc actgacttga agacacggtc caattctggt
    48541 ttgatttggt cttgagagat agtgaaagtc aagacaccac ggtttgtttc tttgttttca
    48601 aatgatacag acattctgtc atttctcctt aaaatttttt aaatacagtc tattataaca
    48661 ttttctataa ataaaatcta gactaatgca gccaattata gacaacaagc ctttttccta
    48721 actaaaacac tcataaagta tagacaattt tagactactt tggatatctt ttggttagtc
    48781 attttccaaa actaatttat ctaatttttc agttatatag ta
//
LOCUS       DBERYS010000010        46730 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.010, whole genome shotgun sequence.
ACCESSION   DBERYS010000010 DBERYS010000000
VERSION     DBERYS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 46730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.010"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..767
                     /locus_tag="ACL8LA_000667"
     CDS             <1..767
                     /locus_tag="ACL8LA_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1812471.1"
                     /translation="LKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRIHLELRNRGYLV
                     NHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEQCYTDVTE
                     FAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSD
                     QGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKSFQSLK
                     QLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(895..1143)
                     /locus_tag="ACL8LA_000668"
     CDS             complement(895..1143)
                     /locus_tag="ACL8LA_000668"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812472.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(1091..2173)
                     /locus_tag="ACL8LA_000669"
     CDS             complement(1091..2173)
                     /locus_tag="ACL8LA_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS1812473.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2299..3081)
                     /gene="gatD"
                     /locus_tag="ACL8LA_000670"
     CDS             complement(2299..3081)
                     /gene="gatD"
                     /locus_tag="ACL8LA_000670"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGS1812474.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3081..4424)
                     /gene="murT"
                     /locus_tag="ACL8LA_000671"
     CDS             complement(3081..4424)
                     /gene="murT"
                     /locus_tag="ACL8LA_000671"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGS1812475.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4523..5839)
                     /locus_tag="ACL8LA_000672"
     CDS             complement(4523..5839)
                     /locus_tag="ACL8LA_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGS1812476.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6032..7258
                     /locus_tag="ACL8LA_000673"
     CDS             6032..7258
                     /locus_tag="ACL8LA_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGS1812477.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7457..9031
                     /locus_tag="ACL8LA_000674"
     CDS             7457..9031
                     /locus_tag="ACL8LA_000674"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1812478.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9296..10084
                     /gene="codY"
                     /locus_tag="ACL8LA_000675"
     CDS             9296..10084
                     /gene="codY"
                     /locus_tag="ACL8LA_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGS1812479.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10084..10659
                     /locus_tag="ACL8LA_000676"
     CDS             10084..10659
                     /locus_tag="ACL8LA_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS1812480.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10741..10826
                     /locus_tag="ACL8LA_000677"
     tRNA            10741..10826
                     /locus_tag="ACL8LA_000677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10775..10777,aa:Leu,seq:aag)
     gene            11109..12239
                     /locus_tag="ACL8LA_000678"
     CDS             11109..12239
                     /locus_tag="ACL8LA_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812481.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(12362..12532)
                     /locus_tag="ACL8LA_000679"
     CDS             complement(12362..12532)
                     /locus_tag="ACL8LA_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812482.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            12740..13498
                     /locus_tag="ACL8LA_000680"
     CDS             12740..13498
                     /locus_tag="ACL8LA_000680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1812483.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13586..14098
                     /locus_tag="ACL8LA_000681"
     CDS             13586..14098
                     /locus_tag="ACL8LA_000681"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGS1812484.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            14280..15224
                     /gene="metA"
                     /locus_tag="ACL8LA_000682"
     CDS             14280..15224
                     /gene="metA"
                     /locus_tag="ACL8LA_000682"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGS1812485.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            15233..15910
                     /locus_tag="ACL8LA_000683"
     CDS             15233..15910
                     /locus_tag="ACL8LA_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1812486.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            16008..16766
                     /gene="tpiA"
                     /locus_tag="ACL8LA_000684"
     CDS             16008..16766
                     /gene="tpiA"
                     /locus_tag="ACL8LA_000684"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGS1812487.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16804..18309
                     /gene="lytC"
                     /locus_tag="ACL8LA_000685"
     CDS             16804..18309
                     /gene="lytC"
                     /locus_tag="ACL8LA_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HGS1812488.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18807..19325
                     /locus_tag="ACL8LA_000686"
     CDS             18807..19325
                     /locus_tag="ACL8LA_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGS1812489.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            19455..19961
                     /locus_tag="ACL8LA_000687"
     CDS             19455..19961
                     /locus_tag="ACL8LA_000687"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGS1812490.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19961..20131
                     /locus_tag="ACL8LA_000688"
     CDS             19961..20131
                     /locus_tag="ACL8LA_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812491.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            20163..21395
                     /gene="clpX"
                     /locus_tag="ACL8LA_000689"
     CDS             20163..21395
                     /gene="clpX"
                     /locus_tag="ACL8LA_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGS1812492.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            21404..21991
                     /gene="yihA"
                     /locus_tag="ACL8LA_000690"
     CDS             21404..21991
                     /gene="yihA"
                     /locus_tag="ACL8LA_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGS1812493.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            22002..22382
                     /locus_tag="ACL8LA_000691"
     CDS             22002..22382
                     /locus_tag="ACL8LA_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGS1812494.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            22434..23324
                     /gene="rapZ"
                     /locus_tag="ACL8LA_000692"
     CDS             22434..23324
                     /gene="rapZ"
                     /locus_tag="ACL8LA_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGS1812495.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            23321..24298
                     /locus_tag="ACL8LA_000693"
     CDS             23321..24298
                     /locus_tag="ACL8LA_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGS1812496.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            24295..25206
                     /gene="whiA"
                     /locus_tag="ACL8LA_000694"
     CDS             24295..25206
                     /gene="whiA"
                     /locus_tag="ACL8LA_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGS1812497.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(25240..26208)
                     /locus_tag="ACL8LA_000695"
     CDS             complement(25240..26208)
                     /locus_tag="ACL8LA_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1812498.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEDYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQTLQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            26344..27201
                     /gene="cdaA"
                     /locus_tag="ACL8LA_000696"
     CDS             26344..27201
                     /gene="cdaA"
                     /locus_tag="ACL8LA_000696"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGS1812499.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            27188..27967
                     /locus_tag="ACL8LA_000697"
     CDS             27188..27967
                     /locus_tag="ACL8LA_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGS1812500.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27991..29343
                     /gene="glmM"
                     /locus_tag="ACL8LA_000698"
     CDS             27991..29343
                     /gene="glmM"
                     /locus_tag="ACL8LA_000698"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGS1812501.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            29417..29791
                     /locus_tag="ACL8LA_000699"
     CDS             29417..29791
                     /locus_tag="ACL8LA_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGS1812502.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29793..30641
                     /locus_tag="ACL8LA_000700"
     CDS             29793..30641
                     /locus_tag="ACL8LA_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1812503.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30918..31685
                     /gene="dapB"
                     /locus_tag="ACL8LA_000701"
     CDS             30918..31685
                     /gene="dapB"
                     /locus_tag="ACL8LA_000701"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGS1812504.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31682..32881
                     /locus_tag="ACL8LA_000702"
     CDS             31682..32881
                     /locus_tag="ACL8LA_000702"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGS1812505.1"
                     /translation="MRLMQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVI
                     KETYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            32878..34749
                     /locus_tag="ACL8LA_000703"
     CDS             32878..34749
                     /locus_tag="ACL8LA_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1812506.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAQDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34892..36076)
                     /gene="mntE"
                     /locus_tag="ACL8LA_000704"
     CDS             complement(34892..36076)
                     /gene="mntE"
                     /locus_tag="ACL8LA_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGS1812507.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVSLLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            36380..39076
                     /locus_tag="ACL8LA_000705"
     CDS             36380..39076
                     /locus_tag="ACL8LA_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS1812508.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            39089..39880
                     /gene="yghU"
                     /locus_tag="ACL8LA_000706"
     CDS             39089..39880
                     /gene="yghU"
                     /locus_tag="ACL8LA_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGS1812509.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLRTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39996..40406
                     /locus_tag="ACL8LA_000707"
     CDS             39996..40406
                     /locus_tag="ACL8LA_000707"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS1812510.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            40418..42013
                     /locus_tag="ACL8LA_000708"
     CDS             40418..42013
                     /locus_tag="ACL8LA_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812511.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGRTFEGQVGKDSYLKTFKVQ"
     gene            42192..42356
                     /locus_tag="ACL8LA_000709"
     CDS             42192..42356
                     /locus_tag="ACL8LA_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGS1812512.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKENKDKFLSILTSLAGLALV
                     LVAVWLGWPK"
     gene            42435..43163
                     /gene="yaaA"
                     /locus_tag="ACL8LA_000710"
     CDS             42435..43163
                     /gene="yaaA"
                     /locus_tag="ACL8LA_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGS1812513.1"
                     /translation="MKILIPTAKEMNTDLPSIETTLLRSESQAVLDALALYSASQLES
                     FYKVSAEKAAEECQNIQALKRQTAQHYPALKLFDGLMYRHIKRDKLTEVEQAYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDEAVKQEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(43214..44698)
                     /locus_tag="ACL8LA_000711"
     CDS             complement(43214..44698)
                     /locus_tag="ACL8LA_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator PRD
                     domain-containing protein"
                     /protein_id="HGS1812514.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            44914..>46730
                     /locus_tag="ACL8LA_000712"
     CDS             44914..>46730
                     /locus_tag="ACL8LA_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS1812515.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETK
                     PAVTTTERSAVDSAILPISTAAKVETTSAPASTNEILKPSLSLSDARQNPAIRKEDVD
                     RGYSGFRTAGSGFRAAGSGFRAAGPENKPILNPNNTIAFSDISQGLHSFRGIGHSRGG
                     REIHYDVTTVRRGNRLNFTIKYSGPGEFVNNNFILDKGDGFGNPSNATITSSNPRVRE
                     QSKSISQGANYVSHSGYSMTSATSTNTEQTIRFSLPIINPNGDLSVRLKPVTFNVDQG
                     GGGAATSNDPYSNSNYYHRANPLLLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQ
                     TRLVREGEKGQRQITYKVHRFGNETLLGLPISNRVTKEAKPRIMQIGVAKELIDTVKP
                     RVDQNKVGDTNNLTFYLDNDGNGVYTEGVDELVQKIAIK"
BASE COUNT        13926 a         8648 c        10550 g        13606 t
ORIGIN      
        1 acttgaaaca gctagacaaa acagataagg accaagagct taaagctgaa attcaatcca
       61 tttttatcga acacaaggga aattatgctt atcgtcgaat tcatttagaa ctaagaaatc
      121 gtggttatct ggtaaatcat aaaagagttc aaggcttgat gaaagtactc aatttacaag
      181 ctaaaatgcg acagaaacga aaatattctt ctcataaagg agacgttggt aagaaagcag
      241 agaatctcat tcaaggccaa tttgaaggct ctaaaacaat ggaacagtgc tacacagatg
      301 tgactgaatt tgccattcca gcaagtactc aaaagcttta cttatcacca gttttagatg
      361 gctttaatag cgaaattatc gcctataatc tttcaacttc acccaactta gaacaagtac
      421 aaacaatgtt ggaacaggca ttcacagaga agcactacga gaatacgatt ctccatagtg
      481 accaaggctg gcaatatcaa cacgattctt atcatcagtt tcttgagggt aagggaattc
      541 aagcatccat gtcacgcaag ggaaacagcc aagacaacgg tatgatggag tccttctttg
      601 ggattctgaa atcggaaatg ttttacggtt atgagaagtc gtttcagtcg cttaagcaat
      661 tggaacaagc tattgtagac tatattgatt actacaacaa taaacgaatt aaggtaaaac
      721 taaaaggact tagtcctgtg caatacagaa ctaaatcctt cggataaatt aattgtctaa
      781 ctgtttgggg gcagtacatt ttaacacttg atactatgcg ttttattgtg ggaagatttt
      841 cctcattttc tagtgaaatt gaactttttc taacatcttt tgatagatga cttcttaatc
      901 aattatcatt tccattgttg tttcttgggg tttcatccct tgtctttgat aaaagcggag
      961 agcaccctcg ttatcattcc agacacttag ggttagatta ttgcagccaa tttctttagc
     1021 ataatagcag aaaaatgaca ataaaatagc ccctagatat tttgaaagga aagcataggg
     1081 gctatatagt ttaatcaaaa taaagcaaat ctttgctggt gcttgtaaag aggtcgaagc
     1141 catccttggt aacaacaccg cagtcttcaa tacgaacacc gactttacca gggatataga
     1201 tacctggttc aacagagaag cacatgcctt cttcgatgac catgtcgttt ccttccatga
     1261 tagatgggaa ttcatggaca tccataccga taccatgccc gagacggtgg ttgaagtact
     1321 caccataacc agctttttcg atgacctcac gggcagcgcg gtccacttca tgagcagtca
     1381 cacctggctt gataaagtca agagcagctt gttgggcttc aagagtcaag ttgtaaatat
     1441 ctttcttgaa ttggtctggt ttgccgacag cgactgtacg agtcatatct gacgcatagc
     1501 cattgaccag aacacccagg tcaaagagga gaagagcatc attttcaacc ttattagctg
     1561 ctggaatgcc gtgtggattc gcagcattat caccagtcaa gaccatggta tcaaagctca
     1621 tttcataacc ttcacgtttc atggcaaagt cgatttgtgc gatgatatct gtctcagtct
     1681 tatcaagaga aatattgtca aaaccaacat gaacagcctt gtcagcataa agacctgcaa
     1741 ccatcatttt ttgcacttca tcagctgatt tgatgaggcg catgcgttgg atacgaggag
     1801 tgaggttgtc aaactcagca gtctcaaaaa ctgttttcaa accatggtat ttggtcaaga
     1861 tgagattgtc aaactcaaca gcgacacgtt tgaagtcaag ttgtggaaga gcatgtttga
     1921 ttttttgcca tggattttca gaatcgacat agcccactac tgggaaggaa acggtgctac
     1981 ttgcacgttc tacttcaaga gctgggacaa agaggagagg ttcctgatcc gctaggacaa
     2041 agaggaacat ttggcgttca tggggatcac tgtaaaaacc agtgaggtaa ttgattgtga
     2101 cggggtcaga tacgacagcg acgtctagtt tttctgattc aagatatgtt aggatttgtt
     2161 gtaatttaga catatgctac cttctttcta cccctctatt ttggcaaaaa atgagagaaa
     2221 atgcaaggga gaagggtaaa agaaaagaca aaaaactccg acaagataac ggagttcttt
     2281 gatatcattt tcctttgttt aagaaaagtc agccttgctt ttgacgtcac tgtactcttc
     2341 agcgatttct tggctgagaa tgtcctcata ggcagggagt tggatgtcct gaccatattt
     2401 cttcttgagg gcagtagtaa ctaggcgata agccagattg gcattacgag agaggatagg
     2461 cccgtggaag taggaaccaa agacattctt ataatgaacc ccttcaccga ccttttcttc
     2521 gttgtttcca tttccataga caacctgtcc cagcggtttt tggtcatcag agaggaaggt
     2581 acgaccttgg tgattttcaa atccatagta ggtttcatcg aaatcttcat tgtgaatctt
     2641 gatgtcaccg ataaaacggt tattggtctg gttgagcgtg tagtgtccca tgacccctag
     2701 cccttcgata cgttttcctg aagcttcaac ataatattga cccaatagtt ggaaaccacc
     2761 gcagatagcc agaactacac cgtcgttttg gatgtagttg tcaatgctct cttttttagc
     2821 aggtaggtcg tctgcaatga tactttgttc aaagtcttga ccaccaccga aaaaggcgat
     2881 gtcgtagtga ttttcatcaa agtcatcatg gagagaaacg atgtcaacgg tcacatgggc
     2941 tcccagtttt tcagccacat acttgagcat gaggatgttt ccattgtccc cgtaggtatt
     3001 catgagattt ccgtagaggt gggcaatgtt gagctgatag gggtaattgc tatcttttga
     3061 ggaaagtgaa gtataaacca ttagttcatc tcctttctaa caatctgacg actagccagc
     3121 agttcacgaa attccagcat ggcagtataa gttgccagaa tataggcatg cttgcagtct
     3181 tgattctcaa tggtcttgag aacttgctcc agattactcg tttcagtgat tttctcagct
     3241 ggatagccag tcactcggag gcgacgagcg atttcagaat gacgaacacc gccagcgttg
     3301 atttcaggaa tgtccatgtc agtgatttgc tcaaagtctg catcccagat ccagctagtg
     3361 tcaattccat ctgcatagtt ggcattaagg aggacagata ggctaaatgg ataaggtgct
     3421 agtttgatca tttcgatagc ttgggttgca ccgactggat ttttaatcaa gacaagggta
     3481 cattccttgt caccgatatg aaaggtttct tggcgtccaa agacagcacg gctcttgtca
     3541 aatccctgtt tgatgagttg ggaatctgcg ccgaggaaac gggcgatggc cacagcagct
     3601 agggcgttat agatattata gagcccgccg atttggatac cgtattcttg gccgtctatg
     3661 acaaagcgag agcgattgtt ggtcaactca accagttttg tcaaacgata gtcgagatca
     3721 ggacgtttac atccacaacc ttcacagata taggcaccca agtttgcata ggtattatgc
     3781 tcatatttga ggatgccttg gcagtcagga cagagaatcc cttcggtatt gtagtgagca
     3841 agttgggctg gtcctttttc caagtcaaaa ccaaaatact ctatagggtt tggaatagtt
     3901 ggcttgtaga aaagtggact gtctccgtta aggagaacag tagcagttgg aacttttcga
     3961 atggcatcca atatcatgtt ataggtagta tagatttcac cgaaacggtc catctggtca
     4021 cggaagatat tagtaatgac aaaaagacta ggctggatat agtcacagat acgagataga
     4081 ctggcttcgt caatttcgag gacggcaata tttttcccag tttttgaaga tttggctgtt
     4141 aggaaggttg ttgcaatccc tgtaatcatg ttggcaccgc ttgggttggt tagaacttga
     4201 ccataaacct cttttaaaat gccgacagtg agggcagttg tcagggtttt tccatttgtt
     4261 ccagtgacaa cgacaatctc gtagttctta gctaggcttt gtaaaatatc tttatcaaat
     4321 tgaagggcga ctttccctgg gagcgtactt ccacgtccaa gacggcttaa aacgaagtgg
     4381 gaagaacgcc cagcaagaag gcccaaagta gtttttaagt tcatgtttct attatatcac
     4441 aaaagaggga aaagacagat ttggtgtttc gtagaagtaa aaacagccca aagagggctg
     4501 tcagttaaga tgagatttca acttaaatcg caaacaagtc attcaagttc tcagccaagg
     4561 ttgggtgagt gaagatttgt tttgtgaagt aagtgtaagg aatcttgttg tccatagcaa
     4621 cagtaatgat gttgatgatt tcttgagaac cttctgagaa gatgcttgca ccaagaattt
     4681 cttttgtttc agtattaaca acagctttga aagctccgcg aaggtctcca tttacgtgac
     4741 cacgaggcat ggctgcaaca gggatttctt tcactgcgta tggaagtttc aaatcagctg
     4801 cttggctttc agtcaaacca acttgtgaaa gtgcaggtgt gatgaacata gtatttggca
     4861 cattgagacg gtcctcaagt gtgtagctgc catctccagc aaggtagctg taaacaacac
     4921 ggaagtcatc aagtgaaatg taagtaaatt gaaggccacc gttgacatct ccaactgcaa
     4981 agacaccagg aacgtttgtt tgacagtgtt tgtctacttt aatagcacca cgttcagtta
     5041 gttcaatatc tgtattttca agttgaagtg gttctacatt tggtttgcgt ccagttgcgt
     5101 agagaagggc gtcgaaacgg taagtttcgt cttcagttac gacaagcact tggtcaccat
     5161 cgtttttgat ttcagtagta tggatatttt gaagcaattc aatgccatct tcttccatgt
     5221 attgtttagc aagagctgcg atggaaggtt ctgcacgagg taggaatgta tccaaggcat
     5281 ctaggactgt gaccttgctt ccaagtttgt tgtaaaggcc ggcaaattca agaccgatat
     5341 ttccgccacc aaggattcca agtttttcag gtaatttgtc caagctttgg atacctgttg
     5401 agtcaaagat gtttttgctt gtagcaagtc caggaattgg caagacgttt gaaacagcac
     5461 cagtgttgat gacgattgtt tcagcagtca gttctttctt ttcatcacca gcttggattt
     5521 cgatgacttt atttgaaagg aagtgagctt ccgcatcaaa gatatctacg cctgtaccag
     5581 caacagtcgc atagttttta ccgttgaggc gaccagtgat cgtgttttta gtagcaatca
     5641 cttcttcaaa agacaagtcc ttttcagcag caactagcaa ggttttagtt gggatacaac
     5701 caatgttgat acaagttcca ccgtacatag ccttgctgcg ttcaacgagg gcaacttttt
     5761 tgccagctga agccaattta cctgctagtg ttttaccagc tttaccaaat ccaataacaa
     5821 ttaaatcgta tgttaacatt tatcttcctc ctattcgaat ttcattctag cacaagaaaa
     5881 aaacttttgc acaattctgc tacggaaaag acagaaaaag ataaaaacgc tttccagaca
     5941 aaaaactagt aattttttga caagcttgat aaactaaaat cctatctatt tttatataag
     6001 aaagaataaa tgttcgaaaa taggaggttt tatgaaatcg aatcgttata ttattgcctt
     6061 tgctggggtc attttacact taatgctggg ttcgacttat gcttggagtg tttatcgtaa
     6121 ccctattatt gaaaaaacgg gatgggatca ggcttctgtt gccttcgcct ttagtctagc
     6181 aatcttttgt ttgggcttat cggctgcatt tatggggcgt ttggtagaaa aatttggtcc
     6241 gaaagtcatg gggagtctat ctgcttttct atacgcaggt ggaaatatct taacaggatt
     6301 tgcaatagac cgtcaggaac tgtggttgtt gtatctagct tatggcattt taggtgggct
     6361 tggtttggga gcaggctata ttacccctgt gtcaacgatt ataaaatggt ttcctgataa
     6421 acgtggtctc gcaacaggtt tagcgattat ggggtttggt tttgcttctt tattgactag
     6481 tcccatagcg caacacctca tcgcaggggt agggattgta gaaacttttt atattttagg
     6541 agcaagttac tttattatca tgctcctagc ttcacaattc attaagcgtc caaatgagca
     6601 agagcttgca attttatctt cttcagggaa agaaaaaaca gcctctttga cgcaaggaat
     6661 ggctgcaaat caggctctaa aaagcaatcg gttttatatg ctttggatta ttttctttat
     6721 caacatagct tgtggtttag gcttaatttc agcggcatcg ccaatggcac aggagatggc
     6781 tggcttgtct acaagtcatg cagcagtaat ggtgggtgtt ttggggattt tcaatggatt
     6841 tggtcgcttg ctctgggcga gtttgtctga ctatatcggt cgccctctaa cctttagtat
     6901 attactgctt gtcaatcttt tcttttctct ctcactttgg ctctttacag attccgtttt
     6961 atttgtagtt gctatgtcta ttttgatgac ttgctatgga gctggttttt ctttgattcc
     7021 agcttatctc agtgatattt ttggaaccaa ggaattggcc gctctgcatg gttatatttt
     7081 aacagcttgg gcaatggctg gtttagcggg acctatttta ttagcagaga cttataaaat
     7141 ggctcattcg tacacacaaa ccttgttcgt ttttctcatt ttatacagta tcgccttggc
     7201 tttgtcttat tatctaggtc gttcaatcaa aaaagaaagt caaaaaccgc ttacatgatt
     7261 ttctgtagaa atggggctat cttttgctct ggtcttgtgt tatactagat aggttgcaaa
     7321 gaaaacagta cttttctttt gtggaaaaga agcaacacga ttttatatct agttatatga
     7381 aaatacgtca taaaaagaaa agtataaact aagccctata gggtggcttc gcaccacctt
     7441 tagaaagaag aataacgtga aatttaatga attaaacttg tctgctgatt tgctagcaga
     7501 aattgaaaaa gctggttttg tagaagctag tcctatccaa gaacaaacta ttcctttggc
     7561 ccttgaaggc aaggatgtta tcggtcaagc tcagactggt acaggaaaaa ctgcagcctt
     7621 tggcttgcct acccttgaaa aaatccgtat agaagaagcg actattcaag ccttggtcat
     7681 cgctccaact cgtgaactag ctgtccaaag tcaagaagaa ctcttccgct ttggtcgtag
     7741 taagggagtc aaagtccgtt cagtatatgg cggatcaagc attgaaaaac aaattaaggc
     7801 tcttaaatct ggtgcccata tcgtggtggg aactccaggt cgcctcttgg acttgattaa
     7861 acgcaaggcc ttgaaattac aagacattga aacccttatc cttgacgaag cggatgaaat
     7921 gcttaacatg ggcttccttg aagacatcga agccattatt tcccgtgtac ctgagaaccg
     7981 tcaaactttg cttttctcag caactatgcc agatgccatc aaacgtatcg gtgttcagtt
     8041 tatgaaagcc cctgaacatg tcaagattgc ggctaaggaa ttgacaacag aattggttga
     8101 ccagtactat atccgtgtta aggaacaaga aaaatttgac accatgactc gtctcatgga
     8161 tgtggcacaa ccagaactcg ctattgtatt tggtcgtacc aaacgccgtg tggatgaatt
     8221 gactcgtggt ttgaaaattc gtggcttccg tgcagaagga attcatggcg acctagacca
     8281 aaacaaacgt cttcgtgtcc ttcgtgactt taaaaatggc aatcttgatg ttttggttgc
     8341 gacagacgtt gcagcgcgtg gtttggatat ttcaggtgtg acccatgtct acaactacga
     8401 tattccacaa gatcctgaga gttatgttca ccgtatcggt cgtacaggtc gtgctggtaa
     8461 gtcaggtcaa tctattactt ttgttgctcc aaacgaaatg ggttaccttc aaatcattga
     8521 aaacttgact aagaaacgca tgaaaggtct caaacctgca agtgtagaag aatccttcca
     8581 atcaaaaaaa caggtagctc tcaagaaaat cgaacgtgat tttgcagatg aaaccattcg
     8641 tgccaacttt gagaaatttg gtaaggatgc tcgcaaattg gctgctgagt ttactccaga
     8701 agaattggca atgtatatct tgagtctgac agtccaagac ccagatagcc ttccagaagt
     8761 ggagattgca cgtgaaaaac cactaccgtt taaaccatca ggtaatggtt tcggtggtaa
     8821 agctaaggga ggtcgtggag gccgtcgtgg ggacgaccgt cgagagcgtg atcgtcgtgg
     8881 caatggtcgc cgtgatgagt tcaaaaaagg aagtcgtggc aacgatcgtt ttgataagga
     8941 aaaacgttac cgtaaggata ataaaaaacc acgcaatact ttaagcgaaa agcaaacagg
     9001 ctttgttatt cgtaacaaag gtgataaata agaaaaagcg gttcaaaatg aatcgctttt
     9061 ttatataagg agaccgaatg tgaaaaagac aaataaaatt attatattat cttttataat
     9121 attatgatac acaaaaatag gcgacttgtc aatagaaaag gaacaattta attaaaaata
     9181 ttctggtttt taattaaatt gtaagtcaat gcaagtcaat aaggaatttt cagataatta
     9241 ttgaaaaaga gacgttttta tgttataatg atagcgatta aattcgaggt gaaaaatggc
     9301 acatttatta gaaaaaacta gaaaaattac atcaattttg aaacgctcag aggagcagtt
     9361 gcaggatgag ctcccttaca acgctattac gcgtcagtta gcggatatta ttcattgcaa
     9421 tgcctgcatt atcaatagta agggacgtct gcttggctat tttatgcgtt ataaaacaaa
     9481 tacagatcgc gtagagcaat tcttccaaac taagattttc ccagatgact acgttcaagg
     9541 ggctaatatg atttacgaaa cagaagcaaa cttgcctgtt gagcatgata tgagtatttt
     9601 ccctgttgag agtagagatg attttccaga tggcttgacg actattgcac cgattcatgt
     9661 atcggggatt cgccttggtt ctttgattat ttggcgtaat gataaaaaat tcgaagatga
     9721 ggacttggtt cttgttgaga ttgccagtac cgttgttggg attcagcttc ttaacttcca
     9781 acgtgaagaa gatgagaaaa atattcgtcg tcgtactgct gtcaccatgg cggtcaatac
     9841 cctttcttac tccgaactcc gtgctgtttc agcaatttta ggggaattaa atggaaatga
     9901 agggaagttg actgcgtcag tgattgcaga tcgtatcgga atcactcgct ctgtgattgt
     9961 caatgctctt cgtaaacttg agtctgcggg gattattgaa agtcgctcac ttggaatgaa
    10021 gggaacctat cttaaggtct tgatttcaga tatttttgaa gaagtgaaga aaagagatta
    10081 ctaatgacaa aggctttaat ttcgattgat tatacagaag attttgttgc tgatagtggg
    10141 aagctgacag caggtgctcc agctcaggcg atttcggatg ccatcagcaa ggtaactcga
    10201 ttagcttttg aacgaggaga ttatatcttc tttactattg atgctcatga agaaaacgat
    10261 tgtttccatc cggaaagtaa gttatttcct cctcataatc tgattgggac gagtggacgg
    10321 aatttatatg gagatttggg gatcttttat caagagcatg gttcagacag tcgtgtcttt
    10381 tggatggata aacgccatta ctcagctttt tcagggactg acctagatat tcgtttgaga
    10441 gagcgtagag tgtctaccgt tatcttaaca ggtgtcttga cggatatctg tgtcctacat
    10501 acagctatag atgcctataa tctaggatat gacatcgaga ttgttaaacc agctgttgct
    10561 tccatctggc ctgaaaatca tcaatttgcc ctaggtcatt tcaaaaatac actcggagct
    10621 aagttagtag atgaaaatct aaatgaactt tctgagtaac ctggttggtg aaaatgaaaa
    10681 aatttgaaaa aaagtgttga caagcatccc aaaagttgat atactagtaa agtaatcgat
    10741 gcggggatgg cggaattggc agacgcgcag gactaaggat cctgtgaccg ctttaggtcg
    10801 tgagggttca agtccctctc tccgcatagg aaaagagtta gctaaagcta gctcttttgt
    10861 atttctctaa aaaattagaa tccagtcaaa tgtagtggag aatcagcgaa aatatgggaa
    10921 aactgatcct tgtttctgct tttgtcatag aagaataata aagattatct tcttcaaatt
    10981 ctccgatatt ctctaaagtt ttgtgcaagt tgcacagaac ttttttattt ttttggtcat
    11041 cttgccatag aaatataaag cgttttcatc tataatataa ttatcaaaag acaaaaggag
    11101 ttcacctcat ggtagaattg aatcttaaaa atatttacaa aaaatatcca aacagcgaac
    11161 actattcagt tgaagatttc aacttgaaca tcaaagataa agaatttatc gttttcgtag
    11221 gaccttcagg atgtggtaaa tcaactacac tccgtatgat tgctggtctt gaagacatca
    11281 cagaaggtac tgcatctatc gatggcgtgg ttgtcaacga cgtagctcca aaagaccgtg
    11341 atatcgccat ggtattccaa aactacgctc tttacccaca catgactgtt tatgacaaca
    11401 tggctttcgg tttgaaattg cgtaaataca gcaaagaaga cattaacaaa cgtgttcaag
    11461 aagcagctga aatacttgga ttgaaagaat tcttggaacg taaaccggct gacctttcag
    11521 gtggtcaacg tcaacgtgtt gccatggggc gtgcgattgt ccgtgatgcg aaagtattct
    11581 tgatggacga acctttgtca aacttggatg ccaaacttcg tgtatcaatg cgtgctgaaa
    11641 tcgctaaaat tcaccgtcgt atcggagcta caactatcta tgtaactcac gaccaaacag
    11701 aagcgatgac acttgcagac cgtatcgtta ttatgtcagc tactaagaac cctgctggta
    11761 caggtactat cggacgtgta gaacaaatcg gtactcctca agaagtttac aaaaatccag
    11821 ttaacaaatt cgttgcagga ttcatcggaa gcccagctat gaacttcatc aacgtgaaat
    11881 tggttggtag cgaaattgtt tctgacggtt tccgtttgaa agtgccagaa ggagcattga
    11941 aagttcttcg tgaaaaaggc tacgaaggaa aagaattgat ctttggtatc cgtccagaag
    12001 acgtgaatgc agaacctgct ttccttgaaa cattcccaga ctgtgttgta aaagcgacta
    12061 tctctgtatc agaactgctt ggttcagaat ctcaccttta ctgccaagtt ggtaaagacg
    12121 agtttgttgc aaaagttgat gctcgtgact acttgcaaac aggtgcaaca gttgagcttg
    12181 gatttgactt gaacaaagca cacttcttcg atgtagaaac tgaaaaaaca atctactaag
    12241 aataaataaa attcaaagca ctacaagaaa agatatctct ttatcaattg tagtggagag
    12301 atatcagtta atctagggag agaaacaaaa tgcttctctc ctttttgcta gggaagtcat
    12361 attatacatc tatattgtga tgctctttat actcttcgaa aatctcttca aaccacgtca
    12421 acgtcgcctt gccgtacgta tgattactga cttcgtcagt tttatctgca acctcaaagc
    12481 tgtactttga gtagcttacg gctagtttcc tagtttgctc tttgatttcc attgagtatt
    12541 atttgtgggt accatctaca agtgaagcta tatgcgtaaa ctacgtgagc aattgaattc
    12601 gaactagaga ggtaataata aatttatgct atagttatgg tgacttgtat gcttttgatt
    12661 ctagtttatc aaataataga ttagaattgt cagataatat cattttgtgt tataatgaag
    12721 aaaaaacaga ggtgttcaaa tgtcagaagc aggtcataag tttttagcaa aattggggaa
    12781 aaaacgctta cgtccaggtg gaaagcgtgc cacagattgg ttaattgcag aaggaggatt
    12841 ttcaaaagaa aagagaatac tagaggttgc gtgtaatagg ggaactacag caattgagtt
    12901 ggcacagcgt tttggttgca agataactgc tgttgatatg gatgctcaag ctttagaagt
    12961 ggctaaaaaa tctgctggaa cggcaggtgt tgctcattta atcagttttg aaagagcaaa
    13021 tgcaatgaaa cttccttatc aagatgctag ttttgatatt gttataaatg aagctatgct
    13081 gactatgcaa gccgatcaag ctaagaaaaa atgtgtaatg gaatatctaa gggtattaaa
    13141 acctggaggt cttctcttga cacatgatgt gcttcttaag gaagctaaag agtctatcag
    13201 acaggaatta tcacaagcaa ttcatgtaaa tgtaggtcct ttaactcaag atggttggga
    13261 acaggtgatg atagaatcag gttattgtga tgtgaaagca ttgactggtg aaatgacatt
    13321 aatgaaatta tcgggtatga tttatgacga aggtttgcta ggaactttga aaatttgtgt
    13381 aaatgcttgt aaaaaggaga atagaaagca gtttttaact atgtataaaa tgtttgctaa
    13441 gaataaacag aaattgggct ttattgcgat ggctagttat aaatcgtcaa aacgttagat
    13501 aattattgaa gttaactttt ccttttttct ttcttaaaaa atatgctata atagagagta
    13561 aaaaactttg aaagaaagaa aaaagatgaa tttaaaagat tacattgcaa caattgaaaa
    13621 ttatccaaag gaaggcatta ccttccgtga tattagtcct ttgatggctg atggaaatgc
    13681 ttatagctac gctgttcgtg aaatcgttca gtatgctact gacaagaaag tcgacatgat
    13741 cgtgggacct gaagctcgtg gatttatcgt gggttgtcca gttgcctttg agttgggaat
    13801 tggttttgcg cctgttcgta agccaggtaa attgccacgc gaagttattt ctgctgacta
    13861 tgaaaaagag tacggtgtcg ataccttgac tatgcacgcg gatgccatta agccaggtca
    13921 acgtgttctt attgtagatg accttttggc gacaggtgga actgttaagg caactatcga
    13981 gatgattgaa aaacttggtg gtgttatggc aggttgtgcc ttccttgttg aattggatga
    14041 attgaacggc cgtgaaaaaa tcggtgacta cgactacaaa gttcttatgc attattaatg
    14101 aaaacagtcc ctagggctgt tttctctaca ctaggatata aaaatagact ataactagtt
    14161 agagaaaaac tataattgaa aactatatct tcttgcagta taataaaagg actaagtgtt
    14221 tgagatttgt cttcaaacac atgcaattat tcctgagaga gtacagttag gagagggtta
    14281 tgccgattcg aattgataaa aaattgccag ctgttgagat tttacggaca gagaatatct
    14341 ttgtcatgga tgatcaacgt gctgcccacc aagatatccg tcctttgaag attttaattt
    14401 taaatctcat gccacagaaa atggtcacag agacccagtt gttgcgccac ttggctaata
    14461 cacccctaca actggatatt gattttctct atatggagag ccaccgttct aaaacaactc
    14521 gttcagagca catggagacc ttctataaaa cttttcctga agtcaaggat gagtattttg
    14581 atgggatgat catcacgggt gctccagttg agcatttacc atttgaggaa gtggactatt
    14641 gggaggaatt tagacagatg cttgagtggt ctaagactca tgtctattcg acccttcata
    14701 tctgttgggg agctcaggct gggctttatc tgcgctatgg tgtagaaaaa taccagatgg
    14761 acagtaagct atcaggtatt tatcctcagg acaccctaaa agagggtcac cttctattta
    14821 gaggctttga tgatagctat gtatcccctc attcacggca cacggagatt tctaaggaag
    14881 aggtcttaaa caagactaat ctcgagattt tatcagaagg acctcaggtt ggggtttcta
    14941 ttttggccag tcgtgattta cgagaaattt atagttttgg tcatttggag tatgaccgtg
    15001 ataccttggc aaaagagtat tttcgagatc gtgatgcagg tttcgaccca catattccag
    15061 aaaattactt taaagatgat gatgttaatc aggtaccttg tctttgttgg tcttcatctg
    15121 cagccctctt tttcagtaat tgggtaaacc atgcggtcta tcaggagacg ccttttgact
    15181 ggagaaagat agaagatgat gcatctgcat atgggtattt ataagaggaa ttatgacata
    15241 tttagacgct tttaaatcag gtaccttggt tttaccgagt gccctgctct tgcattttaa
    15301 ggaactcttt ccttctagcg acgattttct ggtttggcaa tttttctatt tgcaaaatac
    15361 gacaggctta gaagaaatgt cgccaagcca gattgctgaa aggattggca aggaaatttc
    15421 ggatgtcaac cagtccattt ctaatctgac ggaaagggga ctgctccagt atcgtactat
    15481 cgaattaaat ggcgaaattg aattgctctt tgatgctagt ttggccttgg aacgtttgga
    15541 tgacctgttt ggagcagttc attcaagttc agaccagcta acaccacaaa accagctcaa
    15601 ggatttggtg gaaaccttcc agcaggagtt gggacgattg ttgacgcctt ttgagattga
    15661 ggatttgacc aagacactaa aggaagatgg aaccagtgct gacttgatta aggaggctct
    15721 tcgtgaagct gttttgaatg gaaaaccaaa ctggaagtac attcaggcga ttttgagaaa
    15781 ctggcgccat gaaggaatca agagtgtggc tcaaattgag gccaagagag cagaaagaga
    15841 agcaagcaat cctcagttga cacaggtatc tgcagatttc agaaatgcca tggatctctg
    15901 gaaggattaa tccatgcaag taggcttgaa atccgagtaa gatttgcaag ctgtgtataa
    15961 ttgtgataga ataaatagaa aataaattga aaaaagaggt atgtgaaatg tcacgtaaac
    16021 catttatcgc tggtaactgg aaaatgaaca aaaatccaga agaagctaaa gcattcgttg
    16081 aagcagttgc atcaaaactt ccttcatcag atcttgttga agcaggtatc gctgctccag
    16141 ctcttgattt gacaactgtt cttgctgttg caaaaggctc aaaccttaaa gttgctgctc
    16201 aaaactgcta ctttgaaaat gcaggtgctt tcactggtga aactagccca caagttttga
    16261 aagaaatcgg tactgactac gttgttatcg gtcactcaga acgccgtgac tacttccatg
    16321 aaactgacga agatatcaac aaaaaagcaa aagcaatctt tgcgaacggt atgcttccaa
    16381 tcatctgttg tggtgaatca cttgaaactt acgaagctgg taaagctgct gaattcgtag
    16441 gtgctcaagt atctgctgca ttggctggat tgactgctga acaagttgct gcctcagtta
    16501 tcgcttatga gccaatctgg gctatcggta ctggtaaatc agcttcacaa gacgatgcac
    16561 aaaaaatgtg taaagttgtt cgtgacgttg tagctgctga ctttggtcaa gaagtcgcag
    16621 acaaagttcg tgttcaatac ggtggttctg ttaaacctga aaatgttgct tcatacatgg
    16681 cttgcccaga cgttgacggt gcccttgtag gtggtgcgtc acttgaagct gaaagcttct
    16741 tagctttgct tgactttgta aaataatcag taagtagcaa aagctaggtg gaacagcatt
    16801 cagatgtctg ttacattttt tataggagag caaagattga aaacaaaaat tggattagca
    16861 agtatctgtt tactaggctt ggcaactagt catgtcgctg caaatgaaac tgaagtagca
    16921 aaaacttcgc aggatacaac gacagcttca agtagttcag agcaaaatca gtcttctaat
    16981 aaaacgcaaa cgagcgcaga agtacagact aatgctgctg cccactggga tggggattat
    17041 tatgtaaagg atgatggttc taaagctcaa agtgaatgga tttttgacaa ctactataag
    17101 gcttggtttt atattaattc agatggtcgt tactcgcaga atgaatggca tggaaattac
    17161 tacctgaaat caggtggata tatggcccaa aacgagtgga tctatgacag taattacaag
    17221 agttggtttt atctcaagtc agatggggct tatgctcatc aagaatggca attgattgga
    17281 aataagtggt actacttcaa gaagtggggt tacatggcta aaagccaatg gcaaggaagt
    17341 tatttcttga atggtcaagg agctatgatg caaaatgaat ggctctatga tccagcctat
    17401 tctgcttatt tttatctaaa atccgatgga acttatgcta accaagagtg gcaaaaagtg
    17461 ggcggcaaat ggtactattt caagaagtgg ggctatatgg ctcggaatga gtggcaaggc
    17521 aactactatt tgactggaag tggtgctatg gcgactgacg aagtgattat ggatggtgct
    17581 cgctatatct ttgcggcctc tggtgagctc aaagaaaaga aagatttgaa tgtcggctgg
    17641 gttcacagag atggtaaacg ctatttcttt aataatagag aagaacaagt gggaaccgaa
    17701 catgctaaga aaatcattga tattagtgag cacaatggtc gtatcaatga ttggaaaaag
    17761 gttattgatg agaacgaggt ggatggtgtc attgttcgtc taggttatag cggtaaagaa
    17821 gacaaggaat tggcgcataa cattaaggag ttaaaccgtc tgggaattcc ttatggtgtc
    17881 tatctctata cctatgctga aaatgagacc gatgctgaga atgacgctaa acagaccatt
    17941 gaacttataa agaaatacaa tatgaacctg tcttacccta tctattatga tgttgagaac
    18001 tgggaatatg taaataagag caagagagct ccaagtgata cagacacttg ggttaaaatc
    18061 atcaacaagt acatggacac gatgaagcag gcgggttatc aaaatgtgta tgtctatagc
    18121 tatcgtagtt tattacagac gcgtttaaaa cacccagata ttttaaaaca tgtaaactgg
    18181 gtagcggctt atacgaatgc tttagaatgg gaaaaccctt attattcagg agaaaaaggt
    18241 tggcaatata cctcttctga atacatgaaa ggaatccaag ggcgcgtaga tgtcagcgtt
    18301 tggtattaag cgatgatttg aaagagggat gtgatagtag caccctcttt ttctttgttt
    18361 tatgatagtt catcctcgag taaattcaag atcttgctcg gaaatgaagc ttatatagta
    18421 gattgaatat agacaaatac cttgtgattg gtaaaacatt ttagaaattc atttaccttt
    18481 cctaatcgac ttggtttcat cttatttcaa tctattatag tattggggaa tttcttcaaa
    18541 ccacatcagc ttggtcagtt ctacctgcga cctcaaaact tgtgctttgg tcaagctggg
    18601 tttagtttcc tagtttgctg atggatttcc attgactata agcatccaac cctctttttg
    18661 tcttctaaag aattcttaaa ttatcagtct attgcaactt ttctcatgtg ggtcatttgg
    18721 cttgcaattg tctttcctta gtagtatact gaggtagtaa tcattaagaa gtggttacat
    18781 aaaaataatg aatgaggtaa aataaaatgg tagaattgaa aaaagaagca gcaaaagatg
    18841 tcgcaacatt aacaaaagca gcgccagtag cattggcaaa aacaaaggaa gttttgaatc
    18901 aagcagttgc tgatttgtat gtagctcacg ttgctttgca ccaagtgcac tggtatatgc
    18961 atggtcgtgg tttccttgta tggcatccaa aaatggatga gtacatggaa gctcttgatg
    19021 gtcatttaga tgaaatcagc gaacgcttga tcacacttgg tggaagccca ttctctactt
    19081 tgacagagtt ccttcaaaac agtgaaatcg aagaagaagc tggtgaatac cgtaatgttg
    19141 aagaaagctt ggaacgtgtt ctagctattt atcgctacct catcactctt ttccaaaaag
    19201 cgttggatgt gactgacgaa gaaggcgatg atgttacaaa cgatattttt gttggtgcta
    19261 aggctgaact tgagaaaaca gtttggatgc ttgccgcaga acttggacaa gcacctggtt
    19321 tgtaagaaac aagacaacat agataaatct gtaggaaatt tcttacagat tttttctatt
    19381 ttgtaagcta ttccaaacca gtcttttttt gagttttttg ataaaatagt actatcagtg
    19441 aaaaggatgg aagcatgact aagaaaatcg tagctatttg ggcccaggat gaagagggtg
    19501 tgattggtaa ggacaatcgt ctgccttggc atttgccagc agaattgcag cactttaaag
    19561 aaacaactct gaatcatgct atcttgatgg ggcgggtaac ctttgatgga atggggcgtc
    19621 gcttgcttcc aaaacgggag accctgattt tgacgcgtaa tccggaagaa aagatagatg
    19681 gggttgctac ttttcaggac gtccagtctg ttcttgactg gtatcaggct caagaaaaga
    19741 atctctacat tatcggtggg aagcaaattt ttcaggcttt tgaagcttac cttgatgaag
    19801 tgattgtcac tcacattcat gctcgggtgg aaggagatac ctatttccct gaagagcttg
    19861 acttgtctct ttttgagaca gtttcaagca aattttacgc caaagatgag aagaatcctt
    19921 atgattttac catccaatac cgcaagagaa aggaagttta atggaacgga gtttatttgg
    19981 tctattcacg gcttttctgt gtttcatctg ctttctggca ggagcacaag cttttcgtaa
    20041 aaagcgttac gggctttcta tcctcctttg gttaaatgcc tttaccaatc tggtcaatag
    20101 tattcatgct ttttacatga ccttatttta gatagaatga cagtatagaa tggaaggaaa
    20161 tcatgtctac aaatagaaaa aatgatatga tggtttattg ctcattttgt ggcaaaaacc
    20221 aagaagaagt acaaaaaata attgctggca acaatgcttt tatttgtaat gaatgcgtgg
    20281 agttagctca ggaaatcatt cgagaagaat tggttgagga agtcttggca gacttgtctg
    20341 aggtgccaaa accaattgaa ctcctccata tcttgaacca ctatgtaatt ggtcaagatc
    20401 gtgccaagcg tgccttggca gtggcggttt ataaccacta caaacgcatc aatttccacg
    20461 atacacgcga agagtcagaa gatgtggatt tgcagaagtc aaacattttg atgattggcc
    20521 caactggttc agggaaaact ttccttgccc agaccttggc taagagcttg aatgtacctt
    20581 ttgctattgc ggatgcgaca gctctgacgg aggctggtta tgtgggtgag gatgtggaaa
    20641 atatactcct caaactcttg caggttgctg actttaacat cgaacgtgca gagcgtggca
    20701 ttatctatgt ggatgaaatt gacaagattg ccaagaagag tgagaatgtg tctatcacac
    20761 gtgatgtttc tggtgaaggg gtgcaacaag cccttctcaa gattatcgag ggaactgttg
    20821 ctagcgtacc gcctcaaggt ggacgcaaac atccacaaca agagatgatt caagtggata
    20881 caaaaaatat cctcttcatc gtgggtggtg cttttgatgg tattgaagaa attgtcaaac
    20941 aacgtctggg tgaaaaagtc atcggatttg gtcaaaacaa taaggcgatt gacgaaaaca
    21001 gctcatacat gcaagaaatc atcgctgaag acattcaaaa atttggtatt atccctgagt
    21061 tgattggacg cttgcctgtt tttgcggctc ttgagcaatt gaccgttgat gacttggttc
    21121 gcatcttgaa agagccaaga aatgccttgg tgaaacaata ccaaaccttg ctttcttatg
    21181 atgatgttga gttggaattt gacgacgaag cccttcaaga gattgctaat aaagcaatcg
    21241 aacggaagac aggggcgcgt ggacttcgct ccatcatcga agaaaccatg ctagatgtta
    21301 tgtttgaggt gccgagtcag gaaaatgtga aattggttcg catcactaaa gaaactgtcg
    21361 atggaacgga taaaccgatc ctagaaacag cctagaggtg actatggaac ttaatacaca
    21421 caatgctgaa atcttgctca gtgcagctaa taagtcccac tatccgcagg atgaactgcc
    21481 agagattgcc ctagcagggc gttcaaatgt tggtaaatcc agctttatca acactatgtt
    21541 gaaccgtaag aatctcgccc gtacatcagg aaaacctggt aaaacccagc tcctgaactt
    21601 ttttaacatt gatgacaaga tgcgctttgt ggatgtgcct ggttatggct atgctcgtgt
    21661 ttctaaaaag gaacgtgaaa agtgggggtg catgattgag gagtacttaa cgactcggga
    21721 aaatctccgt gcggttgtca gtctagttga ccttcgtcat gccccgtcag cagatgatgt
    21781 gcagatgtac gaatttctca agtattatga gattccagtc atcattgtgg cgaccaaggc
    21841 ggacaagatt cctcgtggta aatggaacaa gcatgaatca gcaatcaaaa agaaattaaa
    21901 ctttgacccg agtgacgatt tcatcctctt ttcatctgtc agtaaggcag ggatggatga
    21961 ggcttgggat gcaatcttag aaaaattgtg aggaaaagaa aatggcaaaa acaattcata
    22021 cagataaggc cccaaaggct atcggaccct atgttcaagg aaaaatcgtt ggcaaccttt
    22081 tgtttgctag cggtcaagtt cccctatccc ctgaaactgg ggaaattgta ggagagaata
    22141 tccaagaaca gacagagcaa gtcttgaaaa acatcggtgc tattttggca gaagcaggaa
    22201 cagactttga ccatgttgtc aaaacaactt gcttcttgag cgatatgaac gactttgttc
    22261 cttttaatga ggtttaccaa acggccttca aagaggaatt cccagctcgt tcagctgtgg
    22321 aggtagctcg tcttcctcgt gatgtaaaag tcgaaattga agtcatcgca gagattggat
    22381 aagctagttg aagtttggtg ttgccaaact tcttttgata taaggagaaa aagatgacaa
    22441 agaaacaact tcacttggtg attgtgacag ggatgagtgg cgcagggaaa actgtagcca
    22501 ttcagtcctt cgaggatcta ggttatttca ccattgataa tatgccgcca gctctcttgc
    22561 ctaagttttt gcagctggtt gaaattaagg aagacaatcc taagttggcc ttggtagtgg
    22621 atatgcgtag ccgttctttc ttttcagaga ttcaagctgt tttggatgag ttggaaaatc
    22681 aagatggttt ggatttcaaa atcctctttt tggatgcggc tgataaggaa ttggtcgctc
    22741 gttacaagga aaccagacgg agtcacccac tagcagcaga cggtcgtatt ttagatggaa
    22801 tcaagttgga acgtgaactc ttggcacctt tgaaaaatat gagccaaaat gtggtggata
    22861 cgactgaact cactccacgt gagctgcgta aaacccttgc agagcagttt tcagaccaag
    22921 aacaagccca gtctttccgt atcgaagtca tgtctttcgg atttaaatat ggaatcccga
    22981 ttgatgcgga cttggtcttt gatgtccgtt tcttgccaaa tccctattat ctaccagaac
    23041 tgagaaacca aacgggtgtg gatgaacctg tttatgatta tgtcatgaac catcctgagt
    23101 cagaagactt ttatcaacat ttattggcct tgattgagcc gattctgcca agttaccaaa
    23161 aggaaggtaa gtccgttttg accattgcta tgggatgtac gggtggacaa caccgtagtg
    23221 tggcatttgc taaacgcttg gtgcaggact tatccaagaa ttggtctgtt aatgaagggc
    23281 atcgcgacaa agaccgcaga aaggaaacgg taaaccgttc atgagaaaac caaagataac
    23341 ggtgattggt ggagggactg gaattcccgt cattctaaaa agtctgcggg aaaaagatgt
    23401 ggaaatcgca gctatcgtga cggtggcaga tgatggtggt tcttcaggtg aactccgaaa
    23461 aaatatgcaa cagttgacac cgccaggtga tcttcgtaat gtccttgtgg ccatgtcgga
    23521 tatgcctaag ttttatgaga aggtctttca gtatcggttc tctgaggatg ccggagcctt
    23581 tgctggccat ccattgggaa atctcatcat tgctggcttg tcagaaatgc agggttcaac
    23641 ctataatgcc atgcagttat tgagcaaatt tttccataca acagggaaaa tttatccttc
    23701 cagtgaccat cctttgaccc ttcatgcagt ctttcaggat gggacagaag tggctggaga
    23761 gagtcatatt gtagaccatc gaggcataat tgacaatgtc tatgtgacca atgccctaaa
    23821 cgatgatacg cctctggcca gccgtcgagt agtgcagacc atccttgaaa gtgacatgat
    23881 tgtcctaggg ccaggttccc tctttacctc tattttgccc aatatcgtga ttaaggaaat
    23941 tgggcgggct cttttggaaa ctaaggcaga aattgcctat gtctgcaata tcatgaccca
    24001 acgtggggag acggaacact ttacagatag cgaccacgtg gaagtcttgc atcgtcacct
    24061 tggtcgccct tttatcgaca ctgtcttggt gaatattgaa aaagtgcctc aggaatacat
    24121 gaattccaac cgttttgatg aatacttagt gcaagtggaa cacgattttg taggtctttg
    24181 taagcaagtt tcgcgcgtga tttcatctaa cttccttcgt ctggaaaatg gcggtgcctt
    24241 ccacgatgga gatttgattg tggacgagtt gatgcgcatt atacaggtga aaaaatgagt
    24301 ttcacagtag cagtaaaaga agaaatcctg ggccaacacc atctgagccg gcatgaatta
    24361 tctgccatta tcaaaatgtc tggtagcatc ggtctctcga cttcgggctt gactttgtca
    24421 gttgtgacag aaaatgccaa actggcccgt cacctctatg agtcctttct ccatttctat
    24481 gaaatcaaat ctgaaattcg tcaccaccaa cggagcaatc ttcgcaagaa tcgcgtctat
    24541 accgttttta cagatgaaaa ggtgcaggat ttgttaagtg atttgcactt ggcagactct
    24601 ttctttggcc tggaaacagg tattgatgag gcgattttat cggacgagga agcaggtcgt
    24661 gcctatctct gtggcgcttt cttggcaaat gggagcattc gtgaccctga gtcaggcaag
    24721 taccagttgg aaatcagttc tgtttatctg gaccacgcgc aaggaattgc ctcccttctc
    24781 caacagtttt tactggatgc caaggtgctt gagcgcaaga agggggctgt gacctatctc
    24841 cagcgagcag aagacattat ggacttcttg atagtaatag gagccatgca ggcacgtgat
    24901 gattttgagc gggttaagat tttgcgagaa acccgtaacg acctcaatcg ggctaataat
    24961 gccgagacag ctaatatcgc tcggacagtt tctgccagca tgaagaccat taacaatatc
    25021 agtaaaatca aagatatcat gggcttagaa aatttgccag tagatttgca ggaagtggcg
    25081 caactgagga ttcagcaccc agactactct atccagcagt tagcagatag tctcagcacc
    25141 cctctgacca aaagtggtgt caatcacaga ctcagaaaaa tcaataagat agcggatgaa
    25201 ttataaaacc acgaatccta tgtgactcgt ggttcttttt tataaactgg tagagtgttt
    25261 tggttgtact ttttgttcag ggtcaatgta gttgatagcg ttgttgacag cagttggagc
    25321 ttctccgagg cctgtcgcaa tcagatcaat ttttccgtca tagtagcagc agtcaccgat
    25381 agcatagata cctgcttggc tggattcctg tttgctgttg acgataatct tgtgacggtt
    25441 gaggtcgagc ccccagtttt taaggttacc gacagaagat ttgaaaccat agttgacaaa
    25501 gaggtggtct aggtcaatgg tttcagtttc atcagatttg acttttgtga tttcaagttt
    25561 atcaagtgtt tttccatttc caaggagttg gctaggggcg aatggtgtct tgatggttac
    25621 agatgattct tgcaaggttt gaacactgtg ttccaaggca cggaaattat ctctgcggtg
    25681 aacaagggta gttggtgcga ttttttcaaa agccaaagcc caatccacag ccgagtctcc
    25741 cccaccaaga atcgtcactt tcttaccagc gtattgctga atgttagaaa cgtggtagtg
    25801 gatattttca tagtcctcaa ccccttcaag ttccagcgga cgtggtttga aggcaccgcc
    25861 acccatagcg atgataactg ttttagtcag gtgacttcct ttggaagttg tgatggcaaa
    25921 ttcttcttct tgtttgtcaa tctcaagaac cgtttcattg agatgaatag gggtatcaaa
    25981 tccatttagc tgttcaatca agcggttagt caactcttct ccagtcaggt ttgggaagcc
    26041 tggtacgtct aggatttcct tttcagggta gagaatagca ggttgtccac ctagctgggg
    26101 aagagagtcg atgatttgaa ccttggcttg gcgtaggtgg gcataaaagg ctgcaaaaag
    26161 cccgacagga ccaccaccca caatggtaat atcatagagt tgagacatag tttctccttt
    26221 gttttttcta gtcagtttat tttatcatat ttttgttcaa tgtaaaatgg actaggatag
    26281 ggaaaaaaat gccagaaaat ggtataatag gacgaaagtg ttttgatagg gaggatatca
    26341 gggatgaatt ttcaacaatt atccaatctg caatattgga ccagtttgtt tgcaagtcca
    26401 tggacgatag ctatcaatct gattgatatt ttgattgttg cttatatttt ataccatttt
    26461 acaaaagcta ttgcaggaac caagattatg attttggtac gtggagtttt ggtgtttatt
    26521 ttagctcaaa tccttgcaaa tatgattggt ttgactacga tttcttggtt aatcaatcaa
    26581 attattactt atggggttat tgcggcggtt gttatcttct ctccagagat tcggactggt
    26641 ttggaacgtt tgggaagagc gacagatttc ttttccaatg cccctattag tgctgaggaa
    26701 cagatgattc gtgcctttgt taagtctgtt gaatacatga gtcctcgtaa aatcggggcc
    26761 ttggttgcta ttcagcgtgt acgtaccttg caggagtata tgtcgacagg aattcccttg
    26821 gatgctaaga tttctgcaga acttctcatt aacattttta ttcccaacac tcccctacat
    26881 gacggtgcgg tgattatcaa agaagaacgt atcgctgtga cgtctgccta tctgcccttg
    26941 acaaaaaaca cagggatttc caaggaattt gggaccagac accgggcggc tatcggttta
    27001 tcagaagtct cagatgcctt gacttttgtc gtatcagagg aaacgggagg aatttcgata
    27061 acctataatg gaaggtttaa gcacaaccta acacttgatg aatttgaaac agaattacgt
    27121 gaaatcttac ttccaaaaga ggaagtgggt cttagtttta aagaacgatt gctaggagga
    27181 tggaaacatg aaaaaaaata gtttatatat catatcctca ctcttttttg cttgtgtctt
    27241 atttgtctat gctacggcga cgaattttca aaacagtacc agtgctaggc aggtaaaaac
    27301 ggaaacctat actaatacag taaccaatgt ccctattgac atacgctata atagtgataa
    27361 gtattttatt agcggttttg cttcagaagt atcagtggtc ttgactggtg caaatcgcct
    27421 atcgctagct agtgaaatgc aagaaagtac acgtaaattc aaggttactg ctgacctaac
    27481 agatgccggt gttggaacga ttgaagttcc tttgagcatt gaagatttac ccaatgggct
    27541 gaccgctgtg gcgactccgc aaaaaattac agtcaagatt ggtaagaagg ctcagaagga
    27601 taaggtaaag attgtaccag agattgaccc tagtcaaatt gatagtcggg tacaaattga
    27661 aaatgtcatg gtgtcagata aagaagtgtc gattacgagt gaccaagaga cattggatag
    27721 aattgataag attatcgctg tcttgccaac tagcgaacgc ataacaggta attacagtgg
    27781 ttcagtacct ttgcaggcaa tcgatcgcaa tggtgttgtc ttaccggcag ttatcactcc
    27841 gtttgataca ataatgaagg tgactacaaa accagtagca ccaagttcaa gcacatcaaa
    27901 ttcaagtaca agcagttcat cggagacatc ttcgtcaacg aaagcaacta gttcaaaaac
    27961 gaattaaaaa tagaaaagga ttttataaca atgggtaaat attttgggac tgatggagtc
    28021 cgtggagaag ctaacctaga gctaacacca gaattagcct ttaaactagg acgttttgga
    28081 ggctatgttc ttagtcaaca tgaaacggaa gcgccgaaag tctttgtagg acgtgacaca
    28141 cgtatttcag gggaaatgct ggaatcggcc ttggtggcag gtctcctttc agtagggatt
    28201 cacgtataca aacttggtgt ccttgcaaca ccagcagtag cttacttggt tgaaactgaa
    28261 ggagcaagtg ccggtgtcat gatttctgct agccacaacc cagcccttga taacggaatc
    28321 aagttctttg gcggtgatgg cttcaaacta gatgatgaaa aagaagcaga aattgaagcc
    28381 ttgctagatg ctgaggaaga cactcttcct cgtccaagtg cagaaggctt aggaattttg
    28441 gtagattatc cagaaggctt gcgtaagtat gaaggatacc ttgtttcaac tggaactcct
    28501 cttgatggaa tgaaggttgc cttggataca gctaatggag cagcttctac cagtgcccgt
    28561 caaatctttg cagaccttgg tgcccaattg acggttatcg gggaaacacc agacggtctt
    28621 aacatcaacc ttaatgttgg ttcaacacat ccagaagccc ttcaagaagt ggtcaaagaa
    28681 agtgggtcag ctattggttt ggcctttgat ggagacagtg accgcttgat tgctgttgat
    28741 gagaatggtg acatcgtcga tggtgacaag attatgtaca tcatcggaaa atacctttct
    28801 gaaaaaggac aattggctca aaatacaatt gtgacaactg ttatgtctaa ccttggtttc
    28861 cacaaggcct tgaatcgcga aggtattaac aaggcagtta ctgcagttgg tgaccgctac
    28921 gttgttgaag aaatgagaaa atcaggttac aaccttggtg gtgaacagtc tggtcacgtt
    28981 atcttgatgg attacaatac cacaggtgat ggtcaattat cagcagttca attgactaaa
    29041 atcatgaagg aaactggtaa gagcttatca gagttggcgg cagaagtaac gatttatcca
    29101 caaaaattag ttaatatccg agtggaaaac gtcatgaagg aaaaggccat ggaagtgcca
    29161 gctatcaagg ccatcatcga gaagatggaa gaagaaatgg cggggaacgg ccgtatcctt
    29221 gttcgtccaa gtggaacaga acccctcttg cgtgttatgg cagaagcgcc tacaacagaa
    29281 gaagtagact actatgttga taccatcaca gatgtagttc gtgctgaaat tgggattgac
    29341 taaatcctag aaaactagat gaaaataaaa aattatggta caatagctta taatattgta
    29401 gccgatggag aaagacatga atcttaaacg tgaacaagaa tttgttagtc agtatcattt
    29461 tgatgcgcgt aattttgaat gggaaaatga aaatggagct cctgaaacca aggtggatgt
    29521 gaatttccaa ttgctccaac atgaccaaga aaatcaagtg acttccttga ttgtcatctt
    29581 gagctttatg attgtatttg ataagtttgt cattagtgga acaatctcac aagtaaacca
    29641 tatcgatggc cgtattgtca atgaaccaaa tgaattgaac caagaagagg tggaaacctt
    29701 ggctcgtcca tgtttgaaca tgctcaatcg tttgacttac gaagtgactg aaattgcatt
    29761 agacttacca ggaattaatt tggagttcta atatgaaatt agctgttttc acagattcct
    29821 ctgcttatct cagtgcagag atcttgcaaa gagaagactt gtttgtcttg gatattcctg
    29881 tcaatattga tggtgaggaa tatgtcgaag gcatcaatct gtctgctgag gaattttacc
    29941 aaaaaatggc tcaggcttct gaattgccta agaccagtca accaagtatt gccaagttag
    30001 atgagatctt aacttcgctc aaagaacaag gctatacaca tgccttgggg cttttcctat
    30061 cttctggaat ttcaggtttt taccaaaata tccagtatat ggtcgatgac tatgagggct
    30121 taaccattgc tttcccagac actttgatta caagtgctcc cctaggcatc atggttgaaa
    30181 gtgtctttaa ttggcgtgat cagggcgatg attttgccag cattcaggat aagctagcca
    30241 ttcaaatcag ccgtacgtca gcctttatca tggtagatga cttggatcat ttggtaaaag
    30301 gtggacgcct ttcaaatggg gctgccattt tgggcaattt gcttagcatt aagccaatcc
    30361 tttattttaa cgatcaaggt gtgattgaag tttacgaaaa agttcgtact gaaaagaagg
    30421 ccaccaagcg cttaattgaa attatcaagg aaacaacggc ttcaggtcaa taccgggtca
    30481 ttgtcattca tgggaatgct cctgaaaagg ctgaagaatt gcgtcagcac ttgcttgatt
    30541 ttggcttggg ttcggatgtt tcacttgcta catttggtag tgtcattgga acgcacctag
    30601 gagcaggaag tattgctctg ggttatattc cagtgattta gttggcgctt ctaggctagt
    30661 taagatgcct tgtatcttag aaattgaaca cggactacct gcctcttgaa aaaagatgcc
    30721 tgtcttgcct ttcgtggaaa gtcagtgcca ttccctattt ttcagaggca gctaaggtcc
    30781 tttgtatctt gataattgaa cacgcctgga accctgtgtg aaaaagatag ttcttccaag
    30841 aagtagacac ttcttgattg gaattcctat tttcactttg tgttcttacg gactttgtat
    30901 cttagacagg agtagagatg agtattcgag taattattgc cggttttaag ggaaagatgg
    30961 gccaggctgc ttgtcagatg gtattgactg atccagactt ggacttggtg gcagttttgg
    31021 atccttttga gtctgagtca gaatggcagg gtattcctgt tttcaaggat aaggctgatt
    31081 tagctggttt tgaagcggat gtctgggtag attttactac tccagctgtt gcctacgaaa
    31141 atacacgttt tgctcttgaa aatggctttg ctccagtagt tggaacgact ggtttcacga
    31201 gtgaagaaat tgcagagcta aaagaatttt ctcgtgccca agacttgggt ggcctgattg
    31261 cccctaactt tgccttgggt gctgtcttac tcatgcaatt tgcgacgcag gctgccaaat
    31321 atttcccaaa tgtggagatt attgagctcc atcatgacaa gaaaaaggat gctccgagtg
    31381 gaacagccat taaaacagct gagttgatgg cagaggttcg agagtccatt cagcaaggtg
    31441 cagcagatga ggaagagctg attgctggtg ctcgtggtgc tgactttgat ggtatgcgca
    31501 tccactcagt tcgtttgcca ggcttggtag ctcatcagga agtcatcttt ggcaatcagg
    31561 gagaagggtt gaccctccgt catgactcct atgatcgcat ctccttcatg acaggagtca
    31621 atttgggaat taaagaagtt gtcaagcgtc atgagcttgt ctatggatta gaacacttat
    31681 tatgagatta atgcaaatgc cttctgaatt tcagaaggct ttaccagtat tagaaaaaat
    31741 taaagaagca ggctttgagg cttattttgt tgggggctct gttcgagatg cccttctcca
    31801 tagccctatc cacgatgtgg atattgcgac gtcttcttat ccagaagaga ccaagcagat
    31861 ttttccgcga acagccgata tcggaatcga gcatggaacc gtcttggtct tagatgggga
    31921 tgaggagtat gaggtaacaa cctttcggac agaggatgtc tatgtggact atcgcagacc
    31981 cagtgcggtt tcctttgtgc gctcgctaga agaagacctc aaacgccgtg atttcacagt
    32041 caacgccttt gccttggatg agacaggaga aatcgttgac ttgttccatg gtttagaaga
    32101 tttggaaaag caagtcttgc gagcagttgg agtggctagt gagcgtttca acgaagatgc
    32161 tttacggatt atgcgtggtt tccgttttca ggctagtctt ggttttgcac ttgagccaga
    32221 aacatttaaa gctatgaaga ccttgacgcc gcttttggag aaaatttctg tagagcgtac
    32281 cttcgtcgag tttgataaac tcttgctggc tccattttgg agaaggggct tggcttccat
    32341 gattgagagt caagcttatg actatctccc tgatatggca tctagccagg acaagctcaa
    32401 cagactgttt gatttggaga ctgattttac ttttgaatcc tctgaacaag cctgggcggc
    32461 tttactatgg gctttggaga ttgaaaatgc gcagtcattt ttgaaatctt ggaagacctc
    32521 acgccagttt gccaagcaag ttcaggattt gctgattatt ttggctctgc gtgaaaatgg
    32581 agaattgagt aagcgtgatt gttatcgctt tgacttggat ttacttttac aggctgaaaa
    32641 tcttcgtcaa gctcagggaa aagaagtcaa cccacaagtc attaaagaaa cctaccagag
    32701 cttgactatt catgataaga aagaaatcca aatcaacggc ggtattttga tcaaggaata
    32761 tggctatcag ccaggcccag acttgggaga gattttaaca gagattgagt ttgccattgt
    32821 cgatggagaa ttggagaata atcgtgaagc catccatgct tacctgaggg agaaaaaatg
    32881 agtgatttta tcgttgaaaa actaagtaaa tccgttggtg acaagaccgt ttttagggat
    32941 atttccttta ttatccatga cttagacaga attggtttaa tcggtgtcaa tgggactggc
    33001 aagaccaccc ttttggacgt cctttctggt gtttctggat ttgatgggga tgtcagtcct
    33061 ttttcagcta aaaatgatta ccagattggt tacttgactc aggatcctga ttttgatgat
    33121 agaaagacag ttttggatac ggttctatct agtgaactca aggaaatcca gctcattcgt
    33181 gagtatgaat tgattatgct cgactatagt gaggacaagc aggcgcgttt ggaacgtgtc
    33241 atggcagaga tggactctct ccaagcttgg gaaattgaga gtcaggtcaa gaccgttctt
    33301 agcaaattgg gcattcaaga cttatctact cctgttgggg aattgtcagg tggtctgaga
    33361 agacgggtac agttggcaca agtcttactt ggcaaccacg accttttgct tttggatgag
    33421 ccgaccaacc atctggatat tgcgattatt gagtggctga ccctcttttt gaaaaattct
    33481 aagaagaccg tcctttttat cactcacgat cgttatttct tagacgcttt gtcaacacgg
    33541 attttcgagt tggagcgagc aggcttgaca gaatatcagg gcaattacca ggactatgtt
    33601 cgccttaagg cagaacagga cgagcgcgat gcagctctac ttcataaaaa agaacaactc
    33661 tacaaacaag aactggcctg gatgcgcaga caaccgcagg cacgtgcgac caagcaacaa
    33721 gctcgtatca atcgtttcca tgatctgaaa aaggaagttt caggcagtag tgctgagaca
    33781 gacttgacta tgaactttga aaccagtcgg attgggaaga aagtcatcga gtttcaggat
    33841 gtttcctttg cctatgaaaa taagcccatt ttgcaaaatt ttaatctctt agttcaggct
    33901 caagaccgta ttggaattgt tggggacaat ggtgttggaa aatcaaccct acttaacctg
    33961 attgcaggaa gtcttgagcc gacagcagga caagttgtga ttggggaaac tgttcgcatc
    34021 gcctatttct ctcaacaaat tgagggtttg gatgaaagca agcgtgtgat caattacctg
    34081 caggaagtgg cagaggaggt caagaccagt ggtggttcta cgacttccat cgctgagttg
    34141 ctggagcaat tcctcttccc acgttcgacg catgggactt tgattgagaa attgtcaggg
    34201 ggtgagaaaa aacgtcttta tctcctcaaa ctgcttttgg aaaaaccaaa tgttcttctt
    34261 ttagacgagc caaccaatga cctagatatt gcaactttga cagtcttaga gaatttcttg
    34321 caaggttttg caggtcccgt tttaacagtc agtcacgacc gctatttctt ggataaggta
    34381 gcgaccaaga ttctcgcttt tgaggatggc aagattcgtc ctttctttgg tcattacacc
    34441 gactatcttg atgaaaaagc ttttgaaaca gatatggcca atcaagtgca aaaggccgaa
    34501 aaggaaaaag tggtcaaggt tcgagaagac aagaaacgca tgacctacca agaaaagcag
    34561 gagtgggcaa gtattgaagg tgatattgaa accttggaaa aacgtatcgc tgctattgaa
    34621 gaggaaatgc aggctaacgg ctctgacttt ggtaagctgg ctactctcca aaaagaattg
    34681 gatgagaaaa atgaagcact ccttgaaaaa tacgaacgct atgagtatct cagtgaattt
    34741 gatagttaag actattcgaa aatcaaagtg caaactatgt cggcttcaca ttgccgactc
    34801 aagtacagcc tgtgactggc ttcctaattt gcactttgat ttttattgag tataaaagaa
    34861 tagaaaaatc ccgtctcatg aaaggatttc tctattcttt tttggtttct tgatgaaaga
    34921 tattttgcca ggtttcgtgg cgacgccaga tactggtatg gatgatacca tctaactcat
    34981 agcagatgag tttggttttt tgactgatgg aagtaatttg aatgtccttg atagcagaat
    35041 ttaactcttt tttggtctta taagcctctt tatccatctg ctctccatct tggcgaatat
    35101 agacaaaatc atcagtcaag agttcttcta gttggtttcc ttggtcaatc aattgttcac
    35161 gcataagcaa ttttttatag acattgtcta aaatttcatc ctcagggata ggtgctggtt
    35221 cgatatggac atcggtatca aagacgccaa aacgctcctc cagcatagac tcgacctgat
    35281 ccgcgatttc atggctttca aaaacagaca agtcaggatt catctctagt gtaatatcca
    35341 ggtagatgtt gctaccgtag gtgcgacctc tttgcgattt gaccttgctg attttgggaa
    35401 tttccatgat agccttttgg tagtcctcga gcaggcggtc gtcaaagcca tctgaaagac
    35461 taaaggaaga ctcgatgaag atatcatagg cagtcttcaa gataaagaaa gtgatgatga
    35521 tagcaaccag tttatccaca atcggataat taaaactact agctaggatg gcaatggcgg
    35581 ttccaagtga ggtaacagcg tcagaaagat tgtccttagc agctgccttc agcgccttgg
    35641 agttggattt cttactgagg cgagtattgt agagatagac cacaaacata atcgctgcag
    35701 aaatgattcc tagagttgca ccaagaggat caatgaccgt ttcttcccga ctgagaatct
    35761 tttgaatggt atctcttaga acatcgaaac cgacatagaa catgatgata gaagtgatca
    35821 agcttgccaa atcttcaatc ttccaatgac caaaacggtg gtcacggtct gcaggctggc
    35881 gcgccatccg aatcccgatt aagagggata catttccaat gatgtccgat acgttattaa
    35941 aaccatcggc caccaaactg gatgaatgaa ggagatgacc agctgctaat ttggctgcag
    36001 acaagatcaa ataggtcgaa atactgataa tggctccacg ctcagctaac ttgagatttg
    36061 agatagattg cttcattcaa cttcctttct agtcacatag cattctacca ttatactggt
    36121 tttcaaggaa aaattcaaat aaggcagttt ttactctttg aaaactcccc aaaatttggt
    36181 ataatggtac tactcaaggg agtagctggc agaaacctgt gatagtgtcg tcattccgaa
    36241 ttttatgctg aaaagtatgc tttccggccc tatcttaaac agcgagactt gttatgatta
    36301 acaggtctct ttttgtttgt cgtgaaaaga tatgacagaa agagagtctg ttttgcacac
    36361 aatgtcaagg aggagacaca tgtcaaaaga acaaaaacgc caagcgtttt atactcagag
    36421 ccctgaagag gtcttgcagg ctgtggatgc gaccgagcaa ggtttgtcat caagtgaggc
    36481 ggaaaagcgc cttgccgaat ttgggcacaa tgaactcgaa gaaggcgaga aacgatcaat
    36541 cttggtcaaa ttcatcgagc aatttaagga tttgatgatt atcatcctag ttgcggcagc
    36601 aatcttgtca gtcgtgactt ctggtgggga agatatcgca gatgccatta tcatcctagc
    36661 tgtggttatc atcaacgctg cctttggtgt ttaccaagaa ggaaaagctg aagaagctat
    36721 tgaagccctc aaatccatgt ctagtccagt tgcccgcgtt cttcgtgatg gacacatggc
    36781 agagattgac tctaaagaat tggtaccagg cgatatcgtt gcccttgaag caggtgacgt
    36841 ggtaccagcg gacctacgtt tgatagaagc caactctctt aaaattgaag aagcagcctt
    36901 gacaggtgaa tctgtaccag tcgaaaaaga cttgtcagtc gagcttgcga cagatgctgg
    36961 tattggtgac cgtgtcaaca tggccttcca aaactcaaac gtaacctatg gtcgtgggat
    37021 gggtgttgtt gtcaatacag gtatgtacac tgaagttggt catattgctg gtatgcttca
    37081 agatgcggat gagactgata caccactcaa acaaaatttg aacaaccttt ctaaggtatt
    37141 gacctatgct atcttggtca ttgcccttgt tacttttgta gtgggtgtct tcattcaagg
    37201 gaaaaatcca cttggtgagt tgttgacttc tgttgccctt gccgttgcag ccattccaga
    37261 aggacttcct gctatcgtta ccatcgttct ttctcttggt actcaagttt tggccaaacg
    37321 acattccatc gttcgtaagt tgccagcagt tgaaacactt ggttcaactg aaatcatcgc
    37381 ttctgataag actggtacgc tgactatgaa caagatgaca gtcgaaaaag tcttctacga
    37441 tgcggttcta catgactcag ctgatgatat tgaactaggt cttgaaatgc cactacttcg
    37501 ttcagttgtc ttggccaatg atacgaaaat cgatgtggaa ggtaacttga ttggtgaccc
    37561 aaccgaaaca gcctttatcc aatatgcctt ggacaagggc tatgatgtca aaggtttctt
    37621 agagaaatat cctcgtgtag ctgaattgcc atttgactct gaccgtaagc tcatgtcaac
    37681 agttcaccca ttgccagatg gtcgtttcct tgtagcagtc aagggtgcgc cagaccaact
    37741 cttaaaacgt tgtcttcttc gtgataaggc tggggatatt gctccgattg atgagaaggt
    37801 tacaaatctc attcatacaa acaattctga aatggctcat caagccttgc gtgtccttgc
    37861 aggtgcttat aagattatcg atagtattcc agaaaatctc acttctgaag agcttgaaaa
    37921 tgatttaatt tttactggtt tgattgggat gattgaccct gaacgtcctg aagccgctga
    37981 ggctgttcgt gtggctaagg aagcgggaat ccgtccaatt atgattacag gtgaccacca
    38041 agacacagcg gaagccattg ccaaacgttt gggaatcatt gacgcaaacg atacagaagg
    38101 tcacgtttta actggtgctg aactcaatga actgtcagat gaagaatttg aaaaagtcgt
    38161 tggtcaatac tctgtttatg cccgtgtgtc tccagaacac aaggttcgta tcgtcaaggc
    38221 ttggcaaaaa caaggtaaag tcgttgccat gacaggtgat ggtgtcaatg acgcgccagc
    38281 tctgaaaaca gccgatatcg gtatcggtat gggaatcact ggtacagaag tttctaaggg
    38341 ggcttctgac atgattcttg cagatgataa ctttgcgact attatcgtcg cagtggaaga
    38401 aggacgtaag gtcttctcaa acattcaaaa gactattcag tacctacttt ctgctaatac
    38461 tgctgaagta ttaaccatct tcctatcaac cttgtttggt tgggatgtct tgcagccggt
    38521 tcatcttttg tggatcaact tggtaacgga taccttccca gctattgctc ttggtgttga
    38581 acctgcggag cctggtgtca tgaatcataa accacgtgga cgcaaggcaa gcttcttctc
    38641 aggtggtgtt ttgagttcta tcatttatca aggtgtactc caagcagctc ttgttatgag
    38701 tgtttatggc cttgcgattg cttacccagt tcatgtgggt gacaatcatg ctattcatgc
    38761 agatgcccta acgatggcct ttgcaaccct tggtttgatt cagctcttcc atgcctacaa
    38821 tgtcaagtct gtctaccaat ccatcttgac agttggccca ttcaagtcta agacctttaa
    38881 ctggtccatc ttggtatcct ttatccttct catggcaaca atcgttgtag aaccgcttga
    38941 aggaatcttc cacgtaacca aactagactt gtcacaatgg ggaattgtta tggctggaag
    39001 cttttcaatg attatcatcg tcgaaatcgt taagtttatt caacgcaaac tcggttttga
    39061 caaaaatgcg atttaaggga ggtttcggat gtcagcatat caattaccga ccgtatggca
    39121 ggatgaagct agtaatcaag gagcttttac ggggctaaac agaccaacag caggtgcccg
    39181 tttcgaacaa aacttgccaa aaggagaaca agcttttcag ctttattcac tgagaacacc
    39241 aaatggtgtg aaggttacta tcttattgga agaattacta gaagctggtt ttaaggaagc
    39301 ggcttacgac ttgtataaga ttgctatcat ggatggggat caattcggat cagactttgt
    39361 gaagctcaat ccaaattcca agattccagc cttattggac cagtcaggta ctgaagatgt
    39421 aagagtcttt gagtctgctc atattcttct ttaccttgct gagaaatttg gagccttttt
    39481 accaagtaat cctgtggaaa aggtagaagt tttgaattgg ctattctggc aagcaggtgc
    39541 agcacctttt ctaggtgggg gatttggaca tttcttcaat tatgctcctg aaaaattgga
    39601 atatcctatt aaccgtttta cgatggaagt gaaacgccag ttggatttat tggataagga
    39661 attggctcag aaaccttata ttgcaggcaa tgactatacg attgcagata ttgctatctg
    39721 gtcttggtat ggacagttag ttcaaggaaa tctttaccaa ggttctgcaa aattcttgga
    39781 tgcctcaagt tatcaaaatc tagtaaaatg ggcagaaaaa attgccaatc gtccagctgt
    39841 taagcgtggc ttggaagtaa cttatacaga aattaaatag caaaaaagtc atcttcggat
    39901 ggctttttgc tacagttgag ggaaagggtt tgcagttatc ggcttttgtg ctataattgc
    39961 tataatatag taaaaagcaa gaggagtgtg aggaagtgga aaagaaaatt gtgaaggata
    40021 tcttattttt atctcaagtg tctcagccgg caagtcagga ggacctttat cttgccagag
    40081 atttgcagga tacactctta gcaaatcgtg atacctgtgt tggtctagct gccaatatga
    40141 ttggggtgca gaagcgcgtg attatcttta atcttggctt ggttcccgtg gtcatgttta
    40201 acccagtgct tctgtccttt gaaggatctt atgaggcaga agaaggctgt ttgtccttgg
    40261 taggtgtgag atcaactaag cgttatgaaa ccataaggct tgcctatcgt gacagcaagt
    40321 ggcaggaaca gaccattacc ttgacaggct tcccagctca gatttgccag catgagctgg
    40381 atcacttgga aggacgaatt atttaggagg aaagcaaatg aaacgaatag tctttgaact
    40441 tatttttatc gcaacgacct ggtatatctt tttaccgccc cttaacctga ccagctggga
    40501 atttctcttc ttcctctgtg ggcatttgtt agttgtggca atcttatttg gctttggcaa
    40561 ggggataaac cttgtcaaaa cggttcatgt gcgccacggt aaggcggaag ctgccttaaa
    40621 tcttgagggt ttcaaaatca atcggttagg gaaaattctg ttagcttcga ttggaggaat
    40681 tcttctcttg gcagctttgg tttccttggt aacttctagc atgtttcagg ctaaaaatta
    40741 tgccaatgta gtcacggtta cggaaaaaga ctttactgaa tttcctaaga gtgacaccag
    40801 taaggttcct atcctagata gaagtactgc tgaaaaaatt ggagaccgct acttgggttc
    40861 cctaaccgat aaggtgtcgc aatacgtagc ggcagatacc tatacccaat tgacaattga
    40921 tgggaaacct tatcgggtca caccactaga atatgcagac cctatcaaat ggtttaacaa
    40981 tcaagccaag ggaattggtg agtatatcaa ggtggacatg gtaactggaa atgcggattt
    41041 ggtggacttg aagacaccaa tcaagtattc agactcggag tattttaacc gtgatgtcaa
    41101 acgtcacctg cgcttgaagt acccgaccaa aatctttaaa actccatctt ttgaggtgga
    41161 cgatgagggc aatcctttct atgtagcaac ggtttaccaa aagcaatttg gacttgctgt
    41221 tcctcgtcct gcttcagtca ttatcttgga tgctacaaat ggagaaacca aggaatacag
    41281 cttatcagat gttccagaat gggtggacag gatctatcca gcagaggaaa ccattgagca
    41341 aattaactac aacggcaagt acaaggacgg tttcttgaat gccatgattt ccaagaaaaa
    41401 cgtgacccag actaccaagg gctataatta cttgtctatc ggtaatgaca tctatctcta
    41461 cacaggtgtg acgtcggcta atgcggatga gagtaatctt ggtttcatcc ttgaaaatat
    41521 gcgaacagga gaaatcacta agtatagctt ggcttctgcg acagaagaat cagcccgtga
    41581 atcagcagaa ggtgctgttc aggagaaatc ctacaaagca accttcccaa tcctcatcaa
    41641 cctcaatgac aagcctctct acatcatggg cttaaaggac aatgctggct tggtcaaaga
    41701 gtacgccctg gtagacgcag tcgagtacca aaatgttatc gttgctacta cagtggaaga
    41761 gatgctcagc aagtatgcca ataaaaacga ccttgaaatt gacaatgcaa cgacagaaag
    41821 catcaaggga gtagtagcag acctcaaatc agctgttatc aagggcgaca ccgtctactt
    41881 ctttaaagtt gatggcaaga tttacaaggt taaggcctca gtatccgatg accttcctta
    41941 ccttgaaaat ggtagaacct tcgaaggtca agtaggaaaa gacagctatc tcaagacctt
    42001 taaagtacag taatactctg cgaaaatctc ttcaaaccac gtcaacttta tctgcaacct
    42061 caaagctgtg ctttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg
    42121 agtataaaaa aggtttattt aagaaatata tgttatagta aaataaatta agactaatgg
    42181 aggacaaaga aatgggtttt tatttgatgg ttgcttcaat gctactcgga ctgctcgctt
    42241 tgaagatagg tttttctcag ttcaaggaga acaaagataa attcttatct atcttaacaa
    42301 gtttagctgg gctagccctt gttctcgtgg ctgtctggtt aggatggccc aaataaatag
    42361 aaaaacctcg gtcaaaccga ggtttttcag atgaatttct tggttgtggc taaaaaatat
    42421 gctacactat caatatgaag attttaatcc ctacagcaaa agaaatgaat acagacttgc
    42481 cgagtatcga gacaactctt ttaagatcag aaagtcaggc tgtacttgat gccttggctc
    42541 tctattctgc cagtcaattg gaaagtttct acaaggtatc agctgagaag gctgcggaag
    42601 aatgtcaaaa tatccaagct ttgaaaaggc aaactgctca acactaccca gccttgaagc
    42661 tttttgatgg gcttatgtac cgtcatatca agagagacaa actgaccgag gtagaacaag
    42721 cctatcttga aaatcatgtc ttcattacct cggctttgta cggagtcgtt ccagccttgt
    42781 cgcctatggc acctcaccgt ttggattttt tgatgaaatt aaaagtcgct ggtaagactt
    42841 tgaagagcca ttggaaggca gcctatgatg aagctgtgaa gcaggaagaa gtgattttct
    42901 ctctcttgtc atcagagttt gagactgtat tttctaagga aatcagagca aagatggtga
    42961 ccttcaaatt catggaggat agaggcggtc agctgaagat tcactcaact atctccaaga
    43021 aagcgcgcgg ggcctttcta acagctttaa tagaaaatca agtacaaact gtgggggaag
    43081 cacgtcgctt gaactttgct ggatttgttt accgagaaga tttgtcacaa ccacagggat
    43141 tggtttttgt aaaggaagta tagaaatcag ataagagaaa agtcagcacg attatgctga
    43201 cttttttgat aacttataaa gtattggaca aatgaatctc atttgataag gtgttaagat
    43261 gattaaccaa attcatgatt gacagatgat ttcggcaaat aaacttcttg gtaatccctg
    43321 aaatatagaa attagacacg atgatatcgt aatctgtctg atttaaaatc tcaggacttg
    43381 tttttaattc atcccaaata tcgaaggtaa agcggttatt acagtaatag gaaagcatat
    43441 caacaaaggt aagggatata gcatggtcaa agttactgat aatcaaaacc ttgataggtg
    43501 gtcgattttc taacaactga gtagagaggt tttcagcatg ggttaagatg gtgtacatca
    43561 agtgttccaa ctgttcagga tggtcatgct gtccaattgc ctgacggtat tgagccagtt
    43621 cttgacgcgc acttcccata aagtctggga agtaaacttc aaatttttta atcgtcaatg
    43681 ctttttgttc aaataagatt ggggtagaaa agatttcctg tctttcaaag aaagcagtgt
    43741 tgtgtaaatg ccaaatcagc tcatcgtggt tagtaaaagt aatcttatat tgttttgata
    43801 ggcgttctaa tatttgacta agtaattggt aagagtatct tgcttgactg ttatcttcta
    43861 aagagttgaa gaattcttga ggatctagga aaatatcatt ctgaagaaaa gaaataaaaa
    43921 tttgagcgat tgtatctgga gtcatttcaa gcccaaaatg tttagagaaa taagcaagtg
    43981 tctcctggaa atttggaatg ctctttaaaa gaggataata ttctttgtaa aagtggttgg
    44041 ggaggtcaat gaagtgacca tttttaaccc tgtggatact gatagctatc atcagtttgt
    44101 acattctata aattgaaaat cgcattggat aattcgtgat tttatagaag aagtcagcaa
    44161 actctgttaa atcttcctca ggaaggtcag gaaaaggcca atctaggaaa taatagcgct
    44221 cagaaaagta ttgggcaaaa aagtaacgaa tatcgatctc atttccacgt atctcggatg
    44281 gagtaaaaga taattcaatt tgaaactgag atgaaagaac ctttgtaata tttctgccca
    44341 gtcgatagag gttggcacta ctaaagtaaa gattttctat tgttcgataa ataggtagcc
    44401 cctcattgaa gaacatatac tctagtaatt gaaaagattg agaattagca agaaaatatt
    44461 gataaatatc ttctacacta gtgttaactt ctaaatctat catgattccg ttaacagaag
    44521 attggatgtt aatagatgga aaggcaatac gtaattcatt taaatcactt tttaagattc
    44581 gttctgtaca tcctaatttt tcggctagtt catgtaattt catccaatta cgattctgaa
    44641 ttagggtttc cattaagcgt aattgtcttt gaacttttgt ggataaaagg tttctcatac
    44701 gggtatttcc tccataattc attatatctt tttttgtcta agtagtataa aaaaattaga
    44761 gcaagaaaat tgtcgttagt aatcggaaaa attagaataa tataagtggc ttttaagtgc
    44821 aaaaacagaa tatattaatt gttcagaaaa tattgattaa tgcctcaaat agttgtacat
    44881 tgtaaactat aaaatactat ctaggagttt aaaatgaaac attcacataa aaaatcattt
    44941 gactggtata gcatgcaaca acgttattct attcgtaagt attactttgg tgcagttagt
    45001 gtcttgctcg gtaccgcttt ggtattaggt gcagcagcta gtgtccaaac agtacaagcg
    45061 gaagaaaaca aacaagaaac taccaatagt atttctgttg gtaggggaga agcagctact
    45121 aaaccagcag aggtttctgc gtctaataaa gagaaaacct atgcagctcc aactgtagct
    45181 aatccagtag aaacgactcc agttaaaact ggagaagtta ctaaaccagc agaaaaagtt
    45241 gaagaagcaa aagacaaaaa agaggaagta acgcatcaag atgctattga caagtcaaaa
    45301 ttgttaacgg ctctttcgcg tgctaaaaag ttagaaagca agttatatac agaagcaagt
    45361 gctgctaact tgcaaacaag tatccaagct ggtcaaagct tgcttgggaa agcagatgca
    45421 tctgaagctg aattatcagc agcagagtca tctattcaat catctattat tggtctagaa
    45481 cttcgttcta actctaacaa aggaactgta tcagaaacgc ctgtagcaaa gaaagctaat
    45541 atagttgaag cgaaggaaga gactaaacca gctgtaacaa cgactgaacg ttcagctgtt
    45601 gatagcgcta ttttgccaat tagcacagct gctaaagtag aaacaacttc agctccagca
    45661 tctactaatg aaatcttgaa accaagtttg agcctttctg atgctcgcca aaatccagct
    45721 atccgtaagg aagatgttga tagagggtat agtggtttta gaacagcagg aagtggcttt
    45781 agagcggctg gaagtggttt tagagcagct ggaccagaaa ataaaccaat cttaaatcca
    45841 aataatacta ttgcattttc tgacatcagt cagggcttac attcatttcg tggtatagga
    45901 cattcacgtg gtggtcgtga aatccattat gatgtaacga cagtacgtag aggtaataga
    45961 ttgaatttca caattaaata ctctggtcca ggtgaatttg ttaataataa ttttatcttg
    46021 gataaagggg atggatttgg aaatccttca aatgcaacga tcacaagttc aaatccaaga
    46081 gtaagggagc aatcaaaatc tattagtcag ggtgccaact acgtatctca ctcaggatac
    46141 agtatgacct ctgctacttc aacaaatacg gaacaaacta tcagatttag tttgcctatc
    46201 attaatccaa atggtgattt gtctgttcgt ttgaaaccag ttacttttaa cgtggaccaa
    46261 ggtggtggtg gtgccgctac tagcaatgac ccatacagta actccaacta ttatcataga
    46321 gcaaatccac tcctcttgga tgcaaaccct tatggtggta ctaataataa gactgtttca
    46381 gaagacattg acttccaaac tgtctatctt ccaactagta agttaccaga aggtcaaact
    46441 agattagttc gagaaggtga aaaaggacaa cgtcaaatta cctataaagt acatcgattt
    46501 ggtaacgaaa cacttttagg attgccaatt agtaatagag ttactaaaga agctaagcca
    46561 cgtattatgc aaattggtgt ggctaaagag ttaatcgata cagtaaaacc acgtgttgat
    46621 caaaataaag ttggtgatac aaataacctc actttctatc ttgataacga tggaaacggt
    46681 gtttatactg agggcgtaga cgaacttgtt caaaaaattg ctattaaaga
//
LOCUS       DBERYS010000011        45719 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.011, whole genome shotgun sequence.
ACCESSION   DBERYS010000011 DBERYS010000000
VERSION     DBERYS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 45719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.011"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            97..594
                     /locus_tag="ACL8LA_000713"
     CDS             97..594
                     /locus_tag="ACL8LA_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGS1812516.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            594..2249
                     /gene="dnaX"
                     /locus_tag="ACL8LA_000714"
     CDS             594..2249
                     /gene="dnaX"
                     /locus_tag="ACL8LA_000714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGS1812517.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2277..2471
                     /locus_tag="ACL8LA_000715"
     CDS             2277..2471
                     /locus_tag="ACL8LA_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGS1812518.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2621..3391
                     /gene="sufC"
                     /locus_tag="ACL8LA_000716"
     CDS             2621..3391
                     /gene="sufC"
                     /locus_tag="ACL8LA_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGS1812519.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3419..4681
                     /gene="sufD"
                     /locus_tag="ACL8LA_000717"
     CDS             3419..4681
                     /gene="sufD"
                     /locus_tag="ACL8LA_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGS1812520.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4692..5918
                     /locus_tag="ACL8LA_000718"
     CDS             4692..5918
                     /locus_tag="ACL8LA_000718"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGS1812521.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5905..6345
                     /gene="sufU"
                     /locus_tag="ACL8LA_000719"
     CDS             5905..6345
                     /gene="sufU"
                     /locus_tag="ACL8LA_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGS1812522.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6399..7811
                     /gene="sufB"
                     /locus_tag="ACL8LA_000720"
     CDS             6399..7811
                     /gene="sufB"
                     /locus_tag="ACL8LA_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGS1812523.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8010..9251)
                     /gene="pbp3"
                     /locus_tag="ACL8LA_000721"
     CDS             complement(8010..9251)
                     /gene="pbp3"
                     /locus_tag="ACL8LA_000721"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGS1812524.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9354..10463
                     /gene="cozE"
                     /locus_tag="ACL8LA_000722"
     CDS             9354..10463
                     /gene="cozE"
                     /locus_tag="ACL8LA_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGS1812525.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11109..11456
                     /gene="ssrA"
                     /locus_tag="ACL8LA_000723"
     tmRNA           11109..11456
                     /gene="ssrA"
                     /locus_tag="ACL8LA_000723"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11639..11726)
                     /locus_tag="ACL8LA_000724"
     tRNA            complement(11639..11726)
                     /locus_tag="ACL8LA_000724"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11690..11692),aa:Ser,seq:gga)
     gene            11889..12629
                     /locus_tag="ACL8LA_000725"
     CDS             11889..12629
                     /locus_tag="ACL8LA_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1812526.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12626..13537
                     /gene="pfkB"
                     /locus_tag="ACL8LA_000726"
     CDS             12626..13537
                     /gene="pfkB"
                     /locus_tag="ACL8LA_000726"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGS1812527.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13534..15486
                     /locus_tag="ACL8LA_000727"
     CDS             13534..15486
                     /locus_tag="ACL8LA_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS1812528.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15623..17875
                     /locus_tag="ACL8LA_000728"
     CDS             15623..17875
                     /locus_tag="ACL8LA_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGS1812529.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAK
                     EALEYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(18026..18454)
                     /locus_tag="ACL8LA_000729"
     CDS             complement(18026..18454)
                     /locus_tag="ACL8LA_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGS1812530.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            18592..19734
                     /locus_tag="ACL8LA_000730"
     CDS             18592..19734
                     /locus_tag="ACL8LA_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS1812531.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19743..20957
                     /gene="thiI"
                     /locus_tag="ACL8LA_000731"
     CDS             19743..20957
                     /gene="thiI"
                     /locus_tag="ACL8LA_000731"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGS1812532.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21092..21916
                     /locus_tag="ACL8LA_000732"
     CDS             21092..21916
                     /locus_tag="ACL8LA_000732"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HGS1812533.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            21929..23095
                     /locus_tag="ACL8LA_000733"
     CDS             21929..23095
                     /locus_tag="ACL8LA_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS1812534.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            23603..25096
                     /locus_tag="ACL8LA_000734"
     CDS             23603..25096
                     /locus_tag="ACL8LA_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS1812535.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25109..26230
                     /locus_tag="ACL8LA_000735"
     CDS             25109..26230
                     /locus_tag="ACL8LA_000735"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1812536.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            26290..26526
                     /locus_tag="ACL8LA_000736"
     CDS             26290..26526
                     /locus_tag="ACL8LA_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGS1812537.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            26523..26936
                     /locus_tag="ACL8LA_000737"
     CDS             26523..26936
                     /locus_tag="ACL8LA_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGS1812538.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            27054..28019
                     /gene="xerA"
                     /locus_tag="ACL8LA_000738"
     CDS             27054..28019
                     /gene="xerA"
                     /locus_tag="ACL8LA_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGS1812539.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            28053..29102
                     /locus_tag="ACL8LA_000739"
     CDS             28053..29102
                     /locus_tag="ACL8LA_000739"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1812540.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYG
                     GNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVF
                     LLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQFACE
                     IAIKVWRNSLKFSII"
     gene            29132..32482
                     /locus_tag="ACL8LA_000740"
     CDS             29132..32482
                     /locus_tag="ACL8LA_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS1812541.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(32988..33458)
                     /locus_tag="ACL8LA_000741"
     CDS             complement(32988..33458)
                     /locus_tag="ACL8LA_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1812542.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(33475..35748)
                     /locus_tag="ACL8LA_000742"
     CDS             complement(33475..35748)
                     /locus_tag="ACL8LA_000742"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGS1812543.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            36095..39196
                     /locus_tag="ACL8LA_000743"
     CDS             36095..39196
                     /locus_tag="ACL8LA_000743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGS1812544.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVSYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            39279..40286
                     /gene="pfkA"
                     /locus_tag="ACL8LA_000744"
     CDS             39279..40286
                     /gene="pfkA"
                     /locus_tag="ACL8LA_000744"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGS1812545.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            40345..41850
                     /gene="pyk"
                     /locus_tag="ACL8LA_000745"
     CDS             40345..41850
                     /gene="pyk"
                     /locus_tag="ACL8LA_000745"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGS1812546.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(42595..42810)
                     /locus_tag="ACL8LA_000746"
     CDS             complement(42595..42810)
                     /locus_tag="ACL8LA_000746"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGS1812547.1"
                     /translation="MEPKIRNSDYLFIKNTPQIDYNTIGIFQVDGTNYVKKLRQGYLK
                     KTQSRILRHPPRRKQPYSYHRKSCQAI"
     gene            43658..44530
                     /locus_tag="ACL8LA_000747"
     CDS             43658..44530
                     /locus_tag="ACL8LA_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGS1812548.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(44676..45020)
                     /locus_tag="ACL8LA_000748"
                     /pseudo
     CDS             complement(44676..45020)
                     /locus_tag="ACL8LA_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            45163..45475
                     /locus_tag="ACL8LA_000749"
                     /pseudo
     CDS             45163..45475
                     /locus_tag="ACL8LA_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45479..45719
                     /locus_tag="ACL8LA_000750"
                     /pseudo
     CDS             45479..45719
                     /locus_tag="ACL8LA_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT        14055 a         8063 c         9825 g        13776 t
ORIGIN      
        1 tttacccact acaaatatta tagagcccaa aatcctttgt ttactaaaca agggattttt
       61 cttttgtctc tgctcctttt ttgatataat agttctatgt taaaatcaga aaaacaatca
      121 cgttatcaaa tgttaaatga agaattgtcc ttcctattgg aaggcgaaac caatgttttg
      181 gctaatcttt ccaacgccag tgctctcata aaatcacgtt ttcctaatac cgtatttgca
      241 ggcttttatt tgttcgatgg aaaggaattg gttttaggcc ccttccaagg aggtgtttcc
      301 tgcatccgta ttgcactagg caagggtgtt tgtggtgagg cagctcactt tcaggaaact
      361 gttattgttg gagatgtgac gacctatctc aactatattt cttgtgatag tctagctaaa
      421 agtgaaattg tggtgccgat gatgaagaat ggtcagttac ttggagttct ggatctggat
      481 tcttcagaga ttgaggatta cgatgctatg gatcgagatt atttggaaca atttgtcgct
      541 attttgcttg aaaagacaac atgggacttt acgatgtttg aggaaaaatc ttaatgtatc
      601 aagcacttta tcgaaaatat agaagtcaaa acttctccca gttagttggt caagaagttg
      661 tggctaagac tcttaaacaa gcggtggagc aagagaaaat aagtcacgct tatctttttt
      721 ctggtcctcg tggaacggga aaaaccagtg ttgctaaaat ctttgccaag gctatgaact
      781 gtcccaatca agtgggtggc gaaccttgca ataactgcta tatttgtcaa gcagtgacgg
      841 acggtagttt agaagatgtc attgaaatgg atgcagcttc taataatggg gtagatgaaa
      901 ttcgcgaaat tcgtgataaa tctacctatg cgcctagcct tgctcgttat aaggtttata
      961 tcatagatga ggttcacatg ctgtctacag gggcttttaa tgccctccta aagacgctgg
     1021 aagaaccaac acagaatgta gtctttattt tggccactac tgaattgcac aagattcctg
     1081 ctactattct atcccgtgtg caacgttttg agtttaaatc aattaagaca caggatatta
     1141 aggaacatat tcactatatc ttagaaaaag aaaatatcag ttctgaacca gaggctgtgg
     1201 aaatcattgc caggcgggcg gaaggtggaa tgcgggacgc cttgtctatt ttggatcaag
     1261 ccctgagttt gacacaggga aatgagctga cgactgctat ctctgaagaa attactggca
     1321 ccattagcct atcagccttg gatgattatg tggcggcctt gtctcaacag gatgttccca
     1381 aagctttgtc ttgcttgaat cttctttttg acaatggtaa gagcatgact cgttttgtga
     1441 ccgatctttt gcactattta agagacttgt taattgttca aacaggggga gaaaatactc
     1501 atcatagttc agtctttgta gaaaatttgg cacttcctca aaaaaatctg tttgaaatga
     1561 ttcgcttagc aacagtgagt ttagcagata ttaagtctag tttgcagccc aagatttatg
     1621 ctgaaatgat gaccgtccgt ttggcggaaa tcaagcccga accagctcta tcaggagcgg
     1681 ttgaaaatga aattgctacg ctgagacagg aagttgcccg tctcaaacaa gagctttcta
     1741 atgtaggtgc ggttcctaaa caagttgcac cagctcctag tcgaccagct acgggcaaaa
     1801 cagtctatcg tgtcgatcgc aataaagtgc aatctatctt acaagaggcc gtcgaaaatc
     1861 ctgatttagc acgtcaaaat ctaattcgtt tgcagaatgc ctggggagag gtaattgaaa
     1921 gtctaggtgg gccggacaag gctctgctag ttggttctca accggttgct gccaatgaac
     1981 accatgctat tcttgctttt gagtctaact tcaatgctgg tcaaactatg aaacgagaca
     2041 atctcaatac catgtttggt aatatcctca gtcaggcggc aggtttttca cctgagattt
     2101 tagctatttc catggaggaa tggaaagaag ttcgcgcagc cttttcagcc aaagccaaat
     2161 cttctcaaac tgaaaaagaa gtagaagaaa gcctgattcc agaaggattt gaatttttgg
     2221 ctgataaagt gaaggtagag gaagactaaa gaaagatttc atgatacaat aagtttatga
     2281 ataaacaaca atttattatt atggcgctgt ttacagctgc tgagacctat tttttcaatg
     2341 aagcctggat gactggccgc tatattatgg cagccttttg ggcaatttta ctctttagaa
     2401 atttccgagt cagttatgtg atgggcaaaa tcgttgatgt catcgatcag cattttaata
     2461 ggaaagacta gccctcagct tccagacaaa atcaaagcct tttaggcttt tttttgttat
     2521 actagaaaag tatatttata gaatttttgc tctatttctg gggaaatcag acgtttttct
     2581 agtaagtact gtaaaagttt tgaaaaagaa aggaactatc atgtcagtat tagagattaa
     2641 agatcttcac gttgagattg aaggaaaaga aattttaaaa ggggttaacc tgaccctgaa
     2701 aacaggagaa attgccgcta tcatgggacc aaatggtaca ggtaaatcga ctctttctgc
     2761 cgctatcatg ggaaatccaa actatgaagt aactaaaggt gaagttttgt ttgatggcgt
     2821 aaacatcctt gagttggaag tggatgagcg tgcgcgtatg ggacttttcc ttgctatgca
     2881 atacccatca gaaatccctg gaattaccaa tgctgagttt cttcgtgccg ctatgaatgc
     2941 gggtaaagaa ggtgatgaga agatttcagt tcgtgagttt attactaagc tagatgaaaa
     3001 aatggaattg ctcaacatga aagaagaaat ggcagagcgt tacctcaacg aaggcttctc
     3061 tggtggtgag aaaaaacgca atgaaattct tcaacttttg atgttggagc caacatttgc
     3121 tcttttggac gagattgact caggtcttga tattgacgct cttaaagttg tgtctaaagg
     3181 tgtcaatgcc atgcgtggtg aaggttttgg tgctatgatc atcactcact accaacgtct
     3241 tttgaactat atcacacctg atgtggtaca cgtgatgatg gaaggtcgtg ttgtcctttc
     3301 tggtggtcca gaattggctg cgcgtttgga acgtgaagga tacgcaaaat tagctgaaga
     3361 acttggctac gactacaagg aagaattgta atttcctcgt atcttttagg agaagtaaat
     3421 gactagagaa aatattaaac ttttttcaga aatgcacgct gaaccaagct ggttggctga
     3481 tctccgtcaa aaagcttttg acaagattga gactttggaa ttaccagtta ttgagcgtgt
     3541 caaattccac cgttggaatc tgggtgatgg aacgattaca gaaaatgagc catcagcaaa
     3601 tgttccagat ttcacagctt tagatcatca cttgaagttg gtgcaagtag gaactcaaac
     3661 tgttttcgaa caaactccag ttgagttagc tgaacagggt gttgtcttca cagactttca
     3721 ctcagcttta gaagaaattc cagagctgat cgaagaattc ttcatgtcat ctgttaagta
     3781 tgatgatgac aagttggcgg cttaccacac agcttacttt aacagtggtg ctgtactcta
     3841 tattccagat aacgtagaaa tcacagagcc aattgaagga attttctacc aagatagcga
     3901 tagcaatgtg ccgtttaaca agcatattat gattatcgct ggtaaaaatt ctaagattag
     3961 ttatctggag cgtttagagt cacgcggtga aggaagtgac aaagcaactg ccaatatcac
     4021 agtggaagtg attgcacgtt ctggtgcgca agtcaagttt gctgctattg accgtctagg
     4081 tgaaaacgtc actgcctaca ttagccgtcg tggtaaatta ggcaacgatg caagtattga
     4141 ctgggctatc ggtgtcatga acgaaggaaa tgtcgttgct gattttgata gtgacttgat
     4201 tggtaatggt agccatgctg acctcaaggt tgtagctctt tcaagtggtc gtcaggtaca
     4261 agggattgat actcgtgtaa ctaactatgg ctgcaactct atcggaaata tcctacaaca
     4321 tggggttatc cttgaaaaag caactttgac tttcaatggt atcggccaca tcatcaaggg
     4381 tgctaaggga gcagatgccc aacaagagag ccgtgttctc atgctttcag accaagcgcg
     4441 ttcagatgct aacccaattc ttttgattga tgaaaatgac gtcactgcag gacatgcagc
     4501 ctctattggt caggtagatc cagaagatat gtactacctc atgagtcgtg gattggataa
     4561 ggcaactgca gagcgtttgg ttgttcgtgg tttccttgga tctgttatcg tggagattcc
     4621 agtcaaggaa gttcgtgatg aaatgattgc aactatcgaa gagaaattgt caaaacgcta
     4681 aggggaagcc tatgttagat gtagaagcga ttcgcaagga ttttccaatt ttagatcaga
     4741 ttgtcaatga tgaacctctg gtctatctgg acaatgctgc gacgacacaa aaaccactag
     4801 tagttctgaa agctattaac agttactatg agcaggacaa tgccaatgtt caccgtggtg
     4861 tccatacctt agcggaacga gcgacagctt cttatgaagc tgctcgtgaa accattcgta
     4921 agtttattaa tgcaggctct acaaaggaag ttctctttac cagaggaacg acaaccagcc
     4981 ttaactgggt ggcacgcttt gctgaggaaa ttctcactga gggagaccag gtcttgattt
     5041 cagtaatgga acaccattct aatatcattc catggcagga agcttgtcga aagactggag
     5101 cagagcttgt ctatgtctat cttaaagacg gtgccttgga tatggaggat ttgcgagcta
     5161 aattgactga taaggttaaa tttgtttccc tagctcatgc ctccaatgtt cttggtgtgg
     5221 tcaatccgat caaggaaatc actcaattag cccaccaagt tggggcaatc atggtagtgg
     5281 atggtgctca atctacacct catatgaaga ttgatgtcca ggacttggat ctggactttt
     5341 tcgccttttc gggtcacaag atggctggtc cgactggtat cggtgtcctt tacggcaaag
     5401 aaaagtatct tgagcaaatg tctccagtag aatttggcgg cgagatgatt gattttgtct
     5461 acgagcaatc tgctagttgg aaggaattgc cttggaaatt tgaggctgga acgccaaata
     5521 tggcaggagc tattggactt gcgactgcag ttgattatct ggaaaagatt ggtatggatg
     5581 ccgttgaagc tcatgaacag gaattgattg cgtacgtcta tccaaaactg caggcaattg
     5641 agggattgac catttacggt tctcaggatt tggctcaacg ttcgggtgtt attgccttta
     5701 acctaggtga tctccatcct cacgatcttg cgacagctct ggattatgaa ggagtggctg
     5761 ttcgtgctgg tcaccattgt gcgcaaccct tgcttcagta tttggaagtc ccagcaacag
     5821 ctcgtgcaag tttttatatc tataatacca aggcagattg cgacaaacta gtcgatgccc
     5881 tacaaaagac aaaggagttt ttcaatggca ctttctaaac tagatagcct ttatatggca
     5941 gtggtagcag accattcgaa aaatccacat caccaaggga agttagaaga cgctgagcaa
     6001 atcagtctca acaatccgac ttgtggagat gtcatcaatc tctctgtcaa gtttgatgca
     6061 gaggaccgtt tggaagatat tgcttttcta aattcaggat gcacgatttc aactgcttct
     6121 gctagtatga tgacagatgc cgttttagga aaaaccaaac aagaaatttt agaactggcg
     6181 actatttttt ctgaaatggt tcaagggcaa aaagatgagc gtcaagacca acttggagac
     6241 gcggcattct tgtcaggtgt tgccaaattc cctcaaagaa tcaagtgtgc aaccctagct
     6301 tggaatgccc ttaagaaaac aattgaaaat caagaaaaac agtaagacaa gtttcttttg
     6361 tcttatgaat tattagaaat gaagaaagaa aggatattat ggctgaagaa agagtagaac
     6421 caaaaccaat tgaccttggt gaatataaat ttggtttcca tgacgatgta gagcctgtct
     6481 tatcgacagg aaaaggactc aacgaaggtg ttattcgtga attatctgct gctaagggtg
     6541 agcctgagtg gatgttggag ttccgtttga agtcttatga aaccttcaaa aaaatgccca
     6601 tgcaaacttg gggagcagac ttgtcagaga ttgactttga tgacttaatc tactaccaaa
     6661 aaccatctga caaaccagcc cgttcttggg atgatgtacc tgaaaagatt aaagaaacct
     6721 ttgaacgtat cgggattcca gaagctgaac gtgcttattt agcaggggct tctgcccagt
     6781 acgagtcaga agtggtttac cacaacatga aggaagagtt ccaaaaatta ggtattatct
     6841 ttacagatac agattccgca ctcaaggaat acccagactt atttaatcaa tactttgcga
     6901 agttggtacc gccgacagat aacaagttgg cagccctcaa ctcagcagta tggtcgggtg
     6961 gaacttttat ctacgtgcca aaaggtgtca aggtagatat tccacttcaa acttatttcc
     7021 gtatcaataa cgaaaatata ggtcagttcg aacgtacctt gattatcgtt gatgagggag
     7081 caagcgtcca ctacgtagaa ggatgtacag caccaacata ttcaagcaat agcttacacg
     7141 ctgccattgt agaaattttt gctttggacg gagcttatat gcgttataca actatccaaa
     7201 actggtctga taacgtctat aacttggtaa caaagcgtgc taaggctcaa aaggatgcca
     7261 ctgttgagtg gattgatgga aacttgggtg ccaaaacgac tatgaaatat ccatctgttt
     7321 accttgatgg agaaggagcg cgtggtacca tgctctctat cgcctttgct aatgcagggc
     7381 aacaccaaga cacgggtgct aagatgattc acaatgctcc acataccagc tcgtctattg
     7441 tgtctaaatc catcgctaaa ggtggaggaa aggttgacta ccgtggacaa gtcaccttta
     7501 acaagaactc taagaaatct gtttcccaca ttgaatgtga taccattatc atggatgact
     7561 tgtcagcatc agatactatt ccatttaatg aaattcacaa ctcgcaagtg gctttggaac
     7621 acgaagccaa agtatctaag atttcagaag agcaattgta ttatctcatg agccgtggat
     7681 tgtcagaatc tgaggcaact gaaatgattg tcatgggatt tgtagaaccc tttacaaaag
     7741 aacttccaat ggaatacgca gttgagctga accgcttgat tagctatgaa atggagggat
     7801 cagttggata aaatttgatt ttatactctt cgaaagtctc ttcaaaccac gtcagcatcg
     7861 ccttaccgta tgtatggtta ctgacttcgt cagtttcatc tacaacctca aaacagtgtt
     7921 ttgagcaacc tgcggctagc ttcctagttt gttctttgat tttgaagatg aacttgtatc
     7981 aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa gcggacaaac
     8041 tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc taccatttcg
     8101 aaactagggc gctctgtggt gatgtaacct tgaccaatca agtccttgtc ttcataagtc
     8161 aaatggccaa ccactgttcc agcttcaagt ggtgctggga ttgctttgga atcaggtgtg
     8221 aattgaacag attgggaaga ttgattccca acacgttcta ttagatagat atcctctgga
     8281 gccactgcag ttactgtatc ttcttttcca tcttgtacag gggttttgct atcttgatag
     8341 gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata atccattagg
     8401 gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc atttaaaaca
     8461 actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc tccagcctta
     8521 tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat accttctaac
     8581 atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt tttggtgatt
     8641 tctaagactt gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac atcataagca
     8701 ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc tagagtttca
     8761 ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc caagagtttt
     8821 gcccgcatca tatcgacgaa atctttttct gagccagcaa ttttctcagc tagggcaata
     8881 gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac agtataatta
     8941 cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata aggataatca
     9001 gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc atagaccaga
     9061 taaacagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc atccttctca
     9121 tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc aatggtaaaa
     9181 tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa agttaaaaat
     9241 atttttttca tagtagtctt attctatcat aaagaaaaaa aatattcttg ctttaataat
     9301 tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa ctcatgtttc
     9361 gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc atcttttacc
     9421 tatggagaca gatgggatct ttgattaacc cttttgttag cgtgcttaat acaattatga
     9481 ttccattttt attagggggc tttctttatt atttgacaaa ccctattgtt actttcttaa
     9541 ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt actttggtct
     9601 ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag ttatctagtt
     9661 tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata gacttatcta
     9721 attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta aacttatcct
     9781 atgttgatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg agcgtcttgt
     9841 cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg gtttatttct
     9901 tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag agggatcgct
     9961 tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat attagtggag
    10021 tttcgattga cgcaatcatt ataggttgtt tggcttatat tggctatagt attattggtt
    10081 taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt ccttatgtgg
    10141 ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat ccccatagac
    10201 tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat atcttatatc
    10261 ctcgaattgt aggaagtgtt atgaaggttc atccaatcac gattttagtt ttacttttgt
    10321 tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc agtgccaacc tattctatct
    10381 tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata atgaaagaac
    10441 gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt actggaagtg
    10501 gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc acagctaaga
    10561 atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattccgtg tcagggtaag
    10621 ttccactggt tttcatagtc tattaaagtt cgaatgaaac ctatttataa tatattgaaa
    10681 ctagaatagt acacctctaa ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc
    10741 tattcgtact attcttattt cattttacta tattttggtg caataagtga aaagtagtcc
    10801 gaataatata aggattgatt ttatagtttt taaactcaaa tgaattaaaa taaagagagt
    10861 acgaaaattc tcatctgaaa gtattttaga ataattctct tcgtgaattt cttcaaaaca
    10921 gatagcttca tcttaggtat gtgttaattg gatttcgtaa gctctattta caatcgtaaa
    10981 gttatgcact gagaaagttg tagttagtct tccagtttgc taaatttctc gaatagtatc
    11041 ttttttaggt atgtgatttc tttttgcatt tttgagttag ataaggtata atggttttat
    11101 tgtcttttgg ggtcgttacg gattcgacag gcattatgag gcatattttg cgactcgtgt
    11161 ggcgacgtaa acgctcagtt aaatataact gcaaaaaata acacttctta cgctctagct
    11221 gcctaaaaac cagcaggcgt gacccgattt ggattgctcg tgttcaatga caggtcttat
    11281 tattagcgag atacgattaa gccttgtcta gcggcttgat aagagattga tagactcgca
    11341 gtttctagac ttgagttatg tgtcgagggg ttgttaaaat aatacataac ctatggttgt
    11401 agacaaatat gttggcaggt gtttggacgt gggttcgact cccaccggct ccattattcc
    11461 tttgcattct tttgcattcc ttggtaaaac gttgttaaat caacgttttt tatttttatc
    11521 tttggtattc ctttgcattc ttttgctaaa aagggagtca caaacagacc ctattttaaa
    11581 aaaaggatag aaaaaaggat acaacatttg tcgcatccta gaaataatct tttttcgacg
    11641 gaagacatgg gattcgaacc cacgcacgct attacacgcc taccgcgttt ccaacacggc
    11701 ctcttaagcc tcttgagtaa tcttccaata cttactcaaa tagtctacca taaaggctct
    11761 tatcttgcaa taaaaattct agaaataaga aaaaatgata gattttgaaa gaaaatgata
    11821 aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat agtgtaaacg ataacaggag
    11881 gtgattcagt gttaaaaaca gaacgtaaac aactaatttt agaggagtta aatcaacatc
    11941 atgtagtttc tctagaaaaa ttagttagtt tgctagaaac gtcagaatca acggttcgaa
    12001 gagacttgga tgagttggaa gcggaaaaca agcttcgtcg tgtgcatggt ggagcagaac
    12061 tcccccactc cttacaggaa gaagaaacca ttcaagaaaa atctgtcaaa aaccttcaag
    12121 aaaagaagtt gctggctcag aaagcagcct ctctcattaa agaaaaagat gtcatcttta
    12181 tcgatgctgg aacaacaact gcttttttga ttcatgaatt ggtcaataag aatgttacag
    12241 ttgtgaccaa ctccattcac catgccgctc agttggttga aaagcagatt ccaactgtca
    12301 tggttggagg aaacgtcaag acggcgacag atgctagtat cgggggcgtt gctcttaacc
    12361 agattaacca attgcacttt gaccgtgcct ttatcggaat gaatggtgtt gacgatggct
    12421 attatacgac tcctgatatg gaggaaggag ctgtgaaaag agctattttg gagaatgcca
    12481 agcagaccta cgtcttggtg gattcgtcaa aaattggaca aacttgcttt gccaaggtag
    12541 caccactcaa acgcgctatc gttatcacta gtcaagggca tgagctcttg caggttatta
    12601 aggagaaaac ggaggtaata gaagtatgat ttatacagtc acactcaatc catccattga
    12661 ctatatcgtt cgtttggacc aagtcaaagt tggtagtgta aatcgtatgg acagtgatga
    12721 taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc ttgaaacgtt tgaatatacc
    12781 aaatacagcg acgggattta tcggtggctt tactggtaaa tttatcacag atactttagc
    12841 agaggaagaa atcgagacac gttttgtcca ggtggcagaa gatactcgta tcaatgttaa
    12901 aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt ccaactgttg aaccggttca
    12961 gctagaagaa ttgaaagcta ttttatctag tctgacagca gaagatacag ttgtctttgc
    13021 aggttcaagt gctaaaaatc taggcaatgt tatctataag gatttgattt ccttgacgcg
    13081 ccagactggt gcgcaagtgg tctgtgactt tgaaggacag accttaattg atagtttgga
    13141 ctaccagcct cttcttgtaa aaccaaacaa tcatgaactt ggagcgattt ttggggttaa
    13201 actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag ttactggcta agggtgctca
    13261 aaatgttatt atctctatgg ctggtgatgg cgcccttctt gtcacatctg agggagctta
    13321 cttcgctaaa ccaatcaaag gaacagtcaa aaattcagtt ggagctggtg attctatggt
    13381 tgctggattc acaggtgaat ttgtcaaatc aaaagacgca gtagaagcct tcaaatgggg
    13441 agtggcttgc ggaacggcaa ctaccttctc agatgacttg gcaacggcgg aatttattaa
    13501 agaaacatat ggaaaagttg aggtagaaaa aagatgaaaa ttcaagacct attgagaaaa
    13561 gatgtcatgt tgctagattt gcaggcaact gaaaaaacag ctgtcatcga cgagatgatt
    13621 aaaaatttga cagagcacag ttatgtaaca gattttgaaa catttaaaga aggaattttg
    13681 gcgcgtgaag ctttgacttc tactggtttg ggtgatggaa tcgcaatgcc tcacagcaaa
    13741 aacgctgctg tcaaagaagc gacagttcta tttgctaagt caaataaggg tgttgactac
    13801 gagagcttgg atggacaagc aactgacctc ttcttcatga ttgcagctcc agaaggtgcc
    13861 aatgatactc acttggcagc cttggcagaa ttgtctcaat acttgatgaa agacggtttt
    13921 gcagacaaac ttcgtcaagc aacatctgca gaccaagtta tcgaactttt tgaccaagct
    13981 tcagaaaaaa ctgaggaact tgttcaagta cctgctaatg actctggtga ctttatcgta
    14041 gctgttacag cttgtacaac aggtattgcc cacacttaca tggcccaaga agcccttcaa
    14101 aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa ccaacggtgc tagcggtgtt
    14161 ggaaatcaac taactgcaga agatatccgt aaggctaaag ctattatcat tgcagcagac
    14221 aaggccgttg aaatggatcg atttgatgga aaaccattga tcaatcgtcc agttgctgac
    14281 ggtatccgta agacagaaga gctaattaac ttggctcttt caggagatac tgaagtctac
    14341 cgtgccgcta atggtgcaaa agctgcaaca gcctctaacg aaaaacaaag ccttggtggt
    14401 gccttctaca aacacttgat gagtggtgta tctcaaatgt taccattcgt tatcggtggt
    14461 ggtatcatga ttgcccttgc cttcttgatt gacggtgctt tgggtgttcc aaatgaaaac
    14521 cttggcaatc ttggttctta ccatgagtta gcttctatgt tcatgaaaat tggtggagct
    14581 gcctttggtt tgatgcttcc agtctttgcg ggttatgttg cctactctat tgctgaaaaa
    14641 ccaggtttgg tagcaggttt cgtggctggt gctattgcca aagaaggttt tgcctttggt
    14701 aaaattcctt atgccgcagg tggtgaagca acttcaactc ttgcaggtgt ctcatctggt
    14761 ttcctaggtg cccttgttgg tggatttatc gcaggtgcct tggttcttgc catcaagaaa
    14821 tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa tccttctatt gccacttctt
    14881 ggaacaatct tgacaggatt tgttatgcta gctgtgaata tcccaatggc agcaatcaac
    14941 actgctatga atgacttcct aggcggtctt ggaggaggtt cagctgtcct tcttggtatc
    15001 gtccttggtg gaatgatggc tgttgacatg ggtggaccag ttaataaagc agcttatgtc
    15061 tttggtacag gtacgcttgc agcaactgtt tcttcaggtg gttctgtagc catggcagca
    15121 gttatggctg gaggaatggt gccaccactt gcaatctttg tcgcaactct tcttttcaaa
    15181 gataaattta ctaaggaaga acgtaactct ggtttgacaa acatcatcat gggcttgtca
    15241 tttatcactg agggagcgat tccatttggt gccgctgacc cagctcgtgc gattccaagc
    15301 ttcatccttg gttcagcagt agcaggtgga ctcgttggtc ttactggtat taaactcatg
    15361 gcgccacacg gaggaatctt cgttatcgcc cttacttcaa atgctctcct ttacctcgtt
    15421 tctgtcttgg taggagcaat cgtaagtggt gtggtttatg gttacctacg caaaccacaa
    15481 gcataaaaaa tagaaaaatg aaaagattgg accgtttggt gcagtctttt tctctttccg
    15541 aaatgcctgt gaaatatggt ataatagaag aatggcaaac aagaatacaa gtacaacaag
    15601 acggagaccg tctaaagcag aactggaaag aaaagaagcg attcaacgaa tgttgatttc
    15661 gttaggaatt gcgattttat tgattttcgc agccttcaaa ttaggggctg caggtataac
    15721 cctttataat ttaattcgct tgctagtggg tagcctagct tatctggcga tattcggcct
    15781 attaatctat ctcttttttt tcaagtggat acgaaaacag gaaggactct tatctggctt
    15841 tttcaccata tttgctggct tactcttgat ttttgaagcc tacttggttt ggaaatatgg
    15901 tttggacaag tccgttctaa aagggaccat ggctcaggtt gtgacagatc tgactggttt
    15961 tcgaacgact agttttgctg gagggggctt gatcggggtc gctctttata ttccaacagc
    16021 ttttctcttt tcaaatatcg gaacttactt tattggttct atcttgattt tagtgggttc
    16081 tctcctagtc agcccttggt ctgtttacga tattgctgaa tttttcagta gaggctttgc
    16141 caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc tttgtcaaac aagaagaaaa
    16201 agctcgccaa aaggctgaga aagaggctag attagaacaa gaagagactg aaaaagcctt
    16261 actcgatttg cctcctgttg atatggaaac gggtgaaatt ctgacagagg aagctgttca
    16321 aaatcttcca cctattccag aagaaaagtg ggtggaacca gaaatcatcc tgcctcaagc
    16381 tgaacttaaa ttccctgaac aggaagatga ctcagatgac gaagatgttc aggtcgattt
    16441 ttcagccaaa gaagcccttg aatacaaact tccaagctta caactctttg caccagataa
    16501 accaaaagat cagtctaaag agaagaaaat tgtcagagaa aatatcaaaa tcttagaagc
    16561 aacctttgct agctttggta ttaaggtaac agttgaacgg gccgaaattg ggccatcagt
    16621 gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc aaccgcattt ccaatctatc
    16681 agatgacctc gctctagcct tggctgccaa agatgtccgg attgaagcac caatccctgg
    16741 gaaatcccta atcggaattg aagtgcccaa ctccgatatt gccactgtat ctttccgaga
    16801 actatgggaa caatcgcaaa cgaaagcaga aaatttcttg gaaattcctt tagggaaggc
    16861 tgttaatgga accgcaagag cttttgacct ttctaaaatg ccccacttgc tagttgcagg
    16921 ttcaacgggt tcagggaagt cagtagcagt taacggcatt attgctagca ttctcatgaa
    16981 ggcgagacca gatcaagtta aatttatgat ggtcgatccc aagatggttg agttatctgt
    17041 ttacaatgat attccccacc tcttgattcc agtcgtgacc aatccacgca aagccagcaa
    17101 ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat gaactctttg ccaaggtggg
    17161 agttcggaat attgcaggtt ttaatgccaa ggtagaagag ttcaattccc agtctgagta
    17221 caagcaaatt ccgctaccat tcattgtcgt gattgtggat gagttggctg acctcatgat
    17281 ggtggccagc aaggaagtgg aagatgctat catccgtctt gggcagaagg cgcgtgctgc
    17341 aggtatccac atgattcttg caactcagcg tccatctgtt gatgtcatct ctggtttgat
    17401 taaggccaat gttccatctc gtgtagcatt tgcggtttca tcaggaacag actcccgtac
    17461 gattttggat gaaaatggag cagaaaaact tcttggtcga ggagacatgc tctttaaacc
    17521 gattgatgaa aatcatccag ttcgtctcca aggctccttt atctcggatg acgatgttga
    17581 gcgcattgtg aacttcatca agactcaggc agatgcagac tacgatgaga gttttgatcc
    17641 aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga gatgctggtg gtgatccgct
    17701 ttttgaagaa gctaagtctt tggttatcga aacacagaaa gccagtgcat ccatgattca
    17761 gcgtcgtttg tcagttggat ttaaccgtgc gacccgtctc atggaagagc ttgagatggc
    17821 aggtgtcatt ggtccagctg aaggtaccaa gccaagaaaa gtattgcagc aataaaaaat
    17881 agcttctttc caaggttgga aggagctatt ttactggcta ttgattactt ttatactctt
    17941 cgcaaatctc ttcaaaccac gtcagcttcg ccttgccgta ctcaagtact gtctacggct
    18001 agcttcctag tttgctcttt gattttcatt gagtattatt ttctgaagtt ggagtattgg
    18061 gctgtttttc attttcagtg gcaggtttac ttgtagcagg tgtagtagag tcctgagttg
    18121 ctgttttctg atcttctttt ttctcttcct tgacgctaga ttttggcgtt tcttcttgtt
    18181 gtgttttttc ttgagaagtg ttattttcct tatttggagt agtattttcc tgaggggatt
    18241 ctttttgaat ttctttgaca aaggttgtcg tctggcttgt cgtaggttct tttttagtat
    18301 ttttattatt attatccaag gcgttcatga tcgtgatacc cgcggtaatc aaaccaagga
    18361 tactaccgat agtagtaatg gttttttgaa aaacagtaaa gccttttttg atatgttctg
    18421 ttttcggttt tgaagttcta cgatagttag acatacattc tctcctttga ttatgattta
    18481 ttatactctg tttctttaaa aaaatcttaa aaaaagagaa attgcccttt cttgtcgcag
    18541 gctccgtttc atgatacaat agaaggagtg attttagaaa gcgtgagaaa catgatttac
    18601 tttgataatt cggcgacgac caggccctat ccagaagccc ttgaaaccta tatgcaggtg
    18661 gcttcaaaaa ttttaggaaa tccatctagt ctccatcgtt tgggagacca ggcaacacga
    18721 attttagatg cttcccgtca acagattgca gatttaatcg gtaagaaaag tgatgaaatc
    18781 ttctttacat ctggtgggac agaaggggat aactggatca tcaagggagt agcatttgaa
    18841 aaagcccagt ttggcaagca catcatcgta tcagccattg aacatccagc agttaaggaa
    18901 tcagccctct ggttgaaagc tcaaggtttt gaagtggatt ttgctccagt tgataacaaa
    18961 ggatttgtgg atgttgaggc gctagaagat ttgataagac ctgatacgac cctcgtttcc
    19021 atcatggctg tgaacaatga aatcggctct atccaaccta ttgaggctat ttcagaattc
    19081 ttggcagaca agccaactat ttccttccac gttgatgcgg ttcaggcgct tgccaagatt
    19141 ccgactgaaa agtatctgac agaacgagtg gatttcgcga ctttctctgg tcacaagttc
    19201 cacggaatcc gtggtgttgg ttttatctat atcaagtctg gcaagaagat tacgcctctt
    19261 ttaacaggtg gtggtcagga acgtgattac cgttcgacaa ctgaaaatgt ggcaggaatt
    19321 gcagcgaccg ctaaggctct ccgtttatct atggaaaagc tagatatctt taggagcaag
    19381 actgggcaga tgaaggcagt gattcgtcaa gcacttctgg actatccgga tatatttgtc
    19441 ttttcagatg aggaagactt cgcccctcat attctgactt ttggaatcaa gggtgttcgt
    19501 ggtgaggtca ttgttcacgc ctttgaagac tatgatattt tcatctcaac gacctcggct
    19561 tgttcgtcca aggctgggaa accagccgga accctgattg ctatgggagt ggacaaggat
    19621 aaggctcagt cagctgtgcg tcttagtcta gaccttgaaa atgatatgag tcaggtcgag
    19681 cagttcttga ccaagctaaa attaatttac aatcaaacta gaaaagtaag ataggagcat
    19741 tcatgcagta ttcagaaatt atgattcgct acggtgagct gtcaaccaag ggcaaaaatc
    19801 gtatgcgttt catcaataaa cttcgcaata atatttcaga cgttttgtct atctatcccc
    19861 aagttaaggt aacagcagat cgcgaccgtg cccacgctta cctcaatgga gcagattaca
    19921 cagcagtagc agaatcgctc aaacaagtat ttggaattca aaacttttcc cctgtttata
    19981 aggttgaaaa atctgtagaa gttctgaagt ctgctgtcca agagattatg caggacatct
    20041 acaaggaagg catgaccttt aaaatctcta gcaagcgtag cgaccacacc tttgaattgg
    20101 acagtcgtga actcaaccaa acgcttggag gggctgtatt cgaagccatt ccaaacgtac
    20161 aagctcaaat gaaaagtcct gacatcaatc ttcaggtgga gatacgtgaa gaagcagcct
    20221 atctttctta tgaaaccgtt cgaggggcag gtggattacc agtgggaact tctggtaagg
    20281 gcatgctcat gttgtcaggg ggaatcgatt cccctgtagc tggctatcta gcgcttaaac
    20341 gaggggtaga tatagaggca gttcactttg ccagcccacc ttacacgagt cctggtgctc
    20401 ttaaaaaagc ccaagatttg acccgtaaat taaccaaatt tgggggaaat atccagttta
    20461 tcgaagttcc ttttacagag attcaagagg aaatcaaagc caaagcgcca gaagcctacc
    20521 ttatgacctt gacgcgtcgt tttatgatgc gtattactga ccgtattcgt gaggtacgaa
    20581 atggtttagt tatcatcaat ggggaaagtc taggtcaagt agccagccaa acccttgaaa
    20641 gtatgaaggc tatcaatgct gttaccaaca ctcccatcat tcgtcctgtg gttaccatgg
    20701 acaagttgga aatcattgac atcgcccagg aaatcgatac ctttgacatt tcaatccaac
    20761 cgtttgaaga ctgttgtact atttttgcac cagatcgtcc aaaaacaaat cctaaaatta
    20821 agaatgcgga gcagtacgaa gcgcgtatgg atgttgaagg cttggttgag cgagcagtgg
    20881 ctggaatcat gattactgaa atcacacctc aagccgaaaa agatgaagtt gatgacttga
    20941 ttgacaatct gctctaattc agaaaatcca aaagaatagc gaaaatcagt aaaaaaagtt
    21001 agttttttct ctaaaaacag gtaaaaaact aacttttttt atttttatga tataatgata
    21061 taaaattttg aatatagaga gttttctgac aatgaatcaa tcctactttt atctaaaaat
    21121 gaaagaacac aaactcaagg ttccttatac aggtaaggag cgccgtgtac gtattcttct
    21181 tcctaaagat tatgagaaag atacagaccg ttcctatcct gttgtatact ttcatgacgg
    21241 gcaaaatgtt tttaatagca aagagtcttt cattggacat tcatggaaga ttatcccagc
    21301 tatcaaacga aatccggata tcagtcgcat gattgtcgtt gctattgaca atgatggtat
    21361 ggggcggatg aatgagtatg cggcttggaa gttccaagaa tctcctatcc cagagcagca
    21421 gtttggtggt aagggtgtgg agtatgctga gtttgtcatg gaggtggtca agccttttat
    21481 caagcacaaa acaggttggt ttgatggcat gatgacgaca ggttgctcta tgggagccta
    21541 tcatgcactc aatttcttcc tccagcatcc agatgtcttt accaaagtga ttgctctcag
    21601 tggtgtttac gacgcacgtt tctttgtcgg cgattactac aacgatgatg ctatttacca
    21661 aaactcgcca gtagattata tttggaacca aaacgatggc tggtttattg accgttaccg
    21721 tcaggcagag attgtgctgt gtacggggct tggagcctgg gaacaagatg gtttaccatc
    21781 cttttacaag ctcaaagaag cctttgacca gaaacaaatt ccagcctggt ttgctgaatg
    21841 gggacatgat gtcgcccacg actgggaatg gtggcgtaaa caaatgcctt atttcctcgg
    21901 taatctctat ttataaaagg agttacctat gaattacctt gttatttctc cctactatcc
    21961 acaaaacttt caacagttta ccatcgaact agctaataaa ggcatcacag tcttgggaat
    22021 tggtcaagag tcttacgagc aattggatga gcccttgcgc aatagcttga ccgagtattt
    22081 tcgtgttgat aatcttgaga acatagatga agtcaaacgt gcagttgctt ttctctttta
    22141 taaacatggt ccaattgacc gcatcgagtc tcacaatgaa tactggcttg agctagacgc
    22201 aacactcaga gaacaattca atgtttttgg tgccaaacca gaggatctca aaaagacgaa
    22261 atataagtct gaaatgaaga aacttttcaa aaaagcaggt gttcctgtgg tacctggagc
    22321 tgttatcaag acggaagcag atgttgatca agcagtgaaa gaaatcggtc ttccaatgat
    22381 tgccaaacct gataatggag tgggagcagc cgcaaccttt aaacttgaga cagaagacga
    22441 tatcaatcac ttcaagcaag aatgggacca ttcaaccctt tatttctttg aaaaatttgt
    22501 cacttccagc gaaatctgta cctttgacgg gctcgtggac aaggatggaa agattgtctt
    22561 ctcaacaacc tttgactacg cctatacacc gcttgacctc atgatttata agatggacaa
    22621 ttcttattat gtgctcaagg atatggatcc taaactgcgc aagtatgggg aagcaattgt
    22681 caaagaattt ggtatgaaag aacggttttt ccatattgag ttcttccgtg agggggacga
    22741 ttatattacc atcgagtaca ataaccgccc tgcaggtggt tttaccattg atgtttataa
    22801 ctttgctcat tccttggacc tttatcgtgg ctatgcagct attgtcgcag gagaggagtt
    22861 cccggcgtca gactttgaaa ctcagtattg tttggctact tctcgccgtg caaatgctca
    22921 ctatgtttat tcagaagagg atttgcttgc caaatatagc cagcagttca aggttaaaaa
    22981 agtcatgcca gctgccttcg cggaacttca aggagattac ctgtatatgc tgacaactcc
    23041 gagtcgacaa gaaatggatc agatgattgc tgatttcgga caacgtcaag aataagaact
    23101 atcggattaa ggaaattcac tcccttaatc cttttgtttt gtctggtaaa aaataagagc
    23161 atcctaacaa ggtagctatc ataaaacttg ttcgataact atttgaagca ggattaggtg
    23221 gctagaaatt gaattttaac atttcaattt agtcatagta ttgtgttttc gtatccttaa
    23281 atcagctaaa aggatccatg acgacaccta tacgatatag ttttcaggat accaaaccag
    23341 tctattaata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggttgctcaa
    23401 aacactgttt tgaggttgtg gatagaactg acagagtcag tatcatatac ctacggcaag
    23461 gtgaagctga cgtggtttga agagattttc gaagagtata aaatattcag gtgacgcata
    23521 gatatagtta attgaagctt tgtttgaaat ctgataaaat aatgatatta ctaagtttta
    23581 aaaactaaag aaaagggaag atatgattac aggcgaatta aaaaataaaa tcgatcagct
    23641 gtgggaaatt ctttggacag aaggaaacgc aaatccttta acaaatattg aacagttgac
    23701 ttatctctta tttatgaaag atttggatag tgtcgagctt ggacgtgaaa gtgatgctga
    23761 atttctaggg attccttatg agggagtttt tccaaaagat aaacctgaat accgttggtc
    23821 aacttttaaa aatataggag atgctcagga agtttatcgt ttaatgactc aggagatttt
    23881 tccgtttatt aaaaatctca agggggatac agatgataca gccttttcac gatatatgcg
    23941 agaagctatt tttcaaataa ataaacctgc tacgcttcaa aaggcaattt ctatcttaga
    24001 tgtttttcca actaggggat tagatgtaga ttttgataat gacaaacaaa gtattactga
    24061 tatcggagat atctatgaat atctgttatc aaaattgtcg accgcaggta aaaatggaca
    24121 gttccgtaca cctcgtcaca tcatcgatat gatggttgag ttgatgcaac cgactatcaa
    24181 agatatcatc tcagatcccg ctatgggttc tgctggcttc ttagtatctg ctagccgtta
    24241 cttaaagcgt aagaaagatg aatgggaaac caatacagat aatatcaatc attttcataa
    24301 tcagatgttt catggaaatg atacggatac gactatgttg agacttgggg cgatgaacat
    24361 gatgctacat ggagtagaaa atccacaaat cagttacctt gactcgctgt ctcaagataa
    24421 tgaagaagcc gataaatata ctttggtttt agcaaatcct ccttttaagg gctcacttga
    24481 ctacaattca acctctaatg accttcttgc aaccgtaaaa accaaaaaaa cagaattact
    24541 ctttctttct cttttcttgc gaactttaaa accaggtgga cgagcagcag ttatcgtacc
    24601 tgatggtgtc ctttttggtt cgtctaaagc tcataaagga attcgtcagg aaattgtaga
    24661 gaatcataag cttgatgctg taatctcaat gcctagtggt gtgttcaagc cttatgctgg
    24721 agtttcaact gccattctca tctttacaaa aactggtaat ggtggtactg acaaagtctg
    24781 gttttacgat atgaaagcgg atggtttaag tttggatgat aagcgacaac cgattagcga
    24841 caatgatatt ccagatatta tcgaacgctt tcatcatctt gaaaaagaag cagaacgtca
    24901 gagaacggat caatctttct ttgttccagt tgctgagata aaggaaaatg attatgattt
    24961 gtctatcaat aaatataaag agattgagta tgaaaaagtt gagtatgaac caacagaggt
    25021 catcttaaag aaaatcaatg atttagaaaa agaaattcaa gctggcttgg ctgaattaga
    25081 aaaattactc aagtagggag gtggctgtat gaaaaaagtg aagttggggg aagtcttatc
    25141 tctaaaaaaa ggcaagaaag ccactgtact tgctgaacaa acaactctaa gccaacgtta
    25201 tattcaaata gatgatttaa gaaataataa taatttaaaa ttcactgaaa gtttaaatat
    25261 gactgaagca ctcccagatg atattctgat agcatgggat ggagctaatg caggaacagt
    25321 tggttatgga ttatcgggag ctgttggtag tacaattacg gtcttaaaaa agaatgagcg
    25381 atacaaagaa aaaattatat cagattactt gggagtcttt ttggaaagta aatcgcagta
    25441 tttacgagaa cattcaacag gtgcaacaat tcctcattta aacaagaata tattacttga
    25501 tttacaatta gaattgctag gtatcgaaga acaagagaac attatctgta ttcttaatac
    25561 gattaaaggg cttattacta aaagaaaatt gcagttagat gaactaaact tgctcgtcaa
    25621 atcccgattt aacgagatgt ttggggaaaa taaaatattt gaaagcattg ataacttatt
    25681 tgatattata gatggtgata ggggcaaaaa ttatcctaaa tcagatgagt tgtttagtga
    25741 ggagtactgt ttatttttaa atacaaagaa tgttactaaa aacggatttt cattcgatac
    25801 aaagcaattt atcactaaaa caaaggataa attacttcga aaaggcaaac ttgagcgtta
    25861 tgatatagtc ttgacaacaa gaggtactgt tggaaatgta gcgtactacg atgaattaat
    25921 aaaatataaa catttacgta taaattcagg tatggtaata ttacgtccca agacaccaaa
    25981 tctaaatcag aaatttatta tccatgtttt aaggaataat aattatagtc gagtgatatc
    26041 aggaagtgct cagcctcagt taccaattac aaaattaaaa aaaatacttc tccccctccc
    26101 cccactagcc ctccaaaatg agttcgcaga ctttgtagtc caggtcgaca aatcacaatt
    26161 ggcaatccaa aaatctctgg aagaacttga aactttgaag aaatctctga tgcaggagta
    26221 ttttggctga tattctgcca ttgtaattac ggtaatgatt tgttataata cttcaaagga
    26281 ggaaatcaga tggtagtaaa aacaagaaaa caaggaaatt caatcaccat tacgattcca
    26341 agtgaattta atattccaag tggtgttaaa tacgaagcga aattgttacc aagtggtgag
    26401 attatcttta ctcctgaaga attggggcag caggtttctt atgtatctga tgatgccttt
    26461 gacttaaatt tagataaaat atttgacgaa tacgacgatg ttttcaaagc tttggtggaa
    26521 aaatgacaat ctatctgaca gaaaagcaaa ttgaaaaaat aaatgcttta gcaattcaac
    26581 ggtattctcc aaatgagaaa attcaaacag ttagttcttc tgccttaaat atgattgtga
    26641 acttaccaga acaatttgtc tttgggaagt ctctttatcc aacaattttt gataaagcaa
    26701 cgatactatt tgtccaattg ataaagaagc atgtttttgc taatgctaat aaaagaactg
    26761 ctttcttcgt tttggtcaaa tttttacaat taaacggcta tcgtttttct gtaacggtag
    26821 aagaagcagt aaaaatgtgt gtaaccatcg cagtagaagc tttaactgat gaaaaaatga
    26881 caagctactc caaatgggtt tctgaacatt ctgttagaga aaaggtcaaa aagtaaccta
    26941 gtatgctgga tttgaatgag cacaagaaaa taaatgaaca gacaatatta gaattctgta
    27001 atgcagaaac tgatattgtc tctttttatt gatgaataag aaagtgagaa attatggaat
    27061 caaaagttac aattatcatg caagaaatgt tacctctttt aaataatgaa caattactag
    27121 cgttgagaga gagtttagaa catcatctag tagacggaaa aaagcagcag aagtattcga
    27181 ataataacct gttgcaacta tttattaccg ccaagcaggt agagggctgt agctcaaaaa
    27241 caattcgtta ttaccagagg acgattgaaa acttgtttaa tgctattaaa gagtctgtga
    27301 cacaactcac aacagatgat ttaaggagtt atttagcaaa ttaccagtct gaaaaggatt
    27361 gtagtaaggc aaatttagac aatattaggc gtatattgtc ttcttttttt gcttggcttg
    27421 agcaagagga atatatcatt aaaaatccca ttcgacggat aaagaaaatt aagactgagc
    27481 aaaatgtgaa ggaaacttat actgatgaac atttggaaat tatgcgtgat aactgtgaaa
    27541 atttgagaga tttggcaata atagacctac tagcatcgac aggtatgcgt gtaggggagc
    27601 ttgtacagtt gaatcgttca gatattgatt ttgaaaacag agagtgtgtt gtctttggta
    27661 aaggaaagaa ggagagacca gtatattttg acgctcggac gaaaattcat ttaagaaatt
    27721 atcttaacga cagaaaagat agtcaccctg ctctttttgt aacgctagtt ggaaaagccc
    27781 agagacttgg aattgctggt gtagagattc gcttaagaaa gttaggagac aaactcggca
    27841 tacaaaaggt tcacccacat aagttcagaa gaactttagc gactaaggca attgataaag
    27901 gtatgcctat cgaacaagtc caaaaactgc taggtcatag caagattgac acaaccctgg
    27961 cctatgccat ggtcaatcaa aataatgtca agcattcaca ccaaaaattc atctcttaaa
    28021 agcaaatccc gatattccga ggaggtggtt gcatgaagaa agtgaaatta gggcaagtgg
    28081 ctacttttat caatggctat gcttttaaac ctcaagattg gtcatctgaa gggaaagaga
    28141 ttatcagaat acagaatctg actaaaactt caaaagggat aaattattat tcgggtacga
    28201 ttgataaaaa atacattgtt gaagctggtg atattcttat ttcgtggtcg ggtacattag
    28261 gggtttttca gtggtgtgga agatccgcag tattaaatca gcacattttt aaagtggttt
    28321 ttgataagat agatatagat aaatcgtatt ttaaatatgt tgttgaaaaa ggtttacaag
    28381 atgctgtaaa acatactcat ggttccacaa tgaaacattt aactaaaaaa tattttgata
    28441 atattatagt tccatataca aatttgggag agcaacaaag aattgcaagt gagctggatt
    28501 tgttaagcaa gctgatttta aggagacaag aacaacttga agagcttaat ctacttgtaa
    28561 aatcccgatt taacgagatg tttggggatc ctttaaataa taacaagaaa tttgcagtaa
    28621 aaacgggaca acagtgtttt aaattttcaa gtggaaaatt tcttgataaa catgatagag
    28681 tttttgaagg ttatcctgcc tatggtggaa atggcattgc ttggaaaagt cgaaaatatt
    28741 taattgataa tcctactatc atcattggtc gtgtaggtgc atattgtggt aatgtaagaa
    28801 ctacccacgg taaggtatgg ataagtgaca atgctatata tataaaagaa tttaagaatt
    28861 ccgatttcaa tcttgtattt ttattagaat taatgaaagt aattgatttt agtaaatttg
    28921 ctgatttctc aggtcaacca aagataactc aaaaaccatt agaaaatcag aaatatatcc
    28981 tcccccctct agccctccaa aatgagttcg cagactttgt agccctggtc gacaaatcac
    29041 aatttgcttg tgagatagct ataaaagtgt ggagaaatag cttgaaattt agtataatat
    29101 agctaaacta tttgtttaaa gtgagaaaaa aatgggaaat tttagctttc ttttaaaaaa
    29161 tgacgaatat gaatcttttt caaaaccttg cattgaagct gagaatatga ttgctacatc
    29221 aactgtggct actgccttta tggcgcgtcg tgctttagag caggctgtcc attggatata
    29281 tagtcacgat tcatatttag aagctcccta tcgtgctact ctatcttctt tagtatggga
    29341 tgatgatttt agggatatcg tagattctga actccacaag cagatagttc tgttgattcg
    29401 gtggggaaac catgctgctc atggtggtga aattaaggaa cgagaagcga ttttagcttt
    29461 gcatcatttg tatcagtttg ttaattttat cgattattgt tacagcaatg agtttgtgga
    29521 gcgttatttt gatgagaagt gcttaccact ttcagcaaac atcaaatacc gagaaactcc
    29581 acaatctatg ataaagttac aagacagttt accagaactg cctgattttc atgaacagat
    29641 ggctgctcag tccgtagaag ttcaagagac ttatactgaa aaacgtgaga ctgcagcgca
    29701 acggcaagat gtgcctttcc atattgatca attatctgag gcagagacaa gaaagctctt
    29761 tattgatatc gatctccgtt tagcaggatg gatatttgaa gaaaactgtc gtgttgagat
    29821 agccgttgat ggtctcaagc acggttcagg aattggttac tgtgactatg tactttatgg
    29881 taaaaatggg aaaattttag cgattgtaga ggctaaaaaa gcctctgtca atccagaagt
    29941 aggggaagta caggtcaaag aatatgctga agctcttgag aaacatatcg gctatcagcc
    30001 aatttgcttt attacaaatg gcttgaagca ctatatactt gatggtccga accgccgcca
    30061 gattgcaggc ttttactctc aagaagaatt gcaattagtg atggatagac gtcatcttca
    30121 aaaaccactt gaggatattt ctagtaaaat tagggacgat atttccgggc gtcactacca
    30181 aaaacatgcc attgcaagcg tttgtgaagc tttctctaat catcgtagac aggcactttt
    30241 ggttatggca actggggctg ggaaaactcg tacagcagtt tctctagttg atatcttatc
    30301 acgtcataac tgggtaaaaa acgttctctt cttagccgat agaacttcct tggttaagca
    30361 agcctatgat tcgtttagaa aattactccc agatctttcc gtttgtaact tcttagaaga
    30421 taaagaagga gctcaatcaa gtcgcatggt cttttcaact tatccgacca tgattggagc
    30481 gattagtggt caagaagaag taaatcaacg ccctttcact gttgggcatt ttgaccttat
    30541 cataattgac gaatctcacc gttctattta tcagaaatac aagtccattt ttgattattt
    30601 tgatgcaaga attgtaggct taacagctac tccgcgtcaa gatttagata aaaacaccta
    30661 tggattcttt aatttggaga atggggttcc aacatatgca tatgatttgg aagaggctgt
    30721 taaagacgga tatttagtag cctatcattc tatcgaaacc aaactgaaac tacctacgga
    30781 tggtctacat tatgatgatt tgtccgaaga agaaaaggaa cattttgata gcaaatttga
    30841 agacgatagc tgtgaaaaag atattgatgg gagtgtattt aattcctttg ttttcaataa
    30901 aagtacagta gaaattgttt taaatgaact catgacaaga ggaattcaga cagcctcggg
    30961 tgatgaaatt ggtaaaacta ttatttttgc taaaaatcat gatcatgcgg aatatatcag
    31021 aggtattttt aacaaccgct atcctgaaaa agggagcgac tatgctcagg tgattgatta
    31081 tagtattaag cattatcaga ccttgattga tgattttaaa attaaggaga agtatcctca
    31141 aattgcgatt tctgtcgata tgttagatac aggtattgat gtaccagagg ttgttaattt
    31201 agtcttcttc aagaaagtac gctctaaaac taagttttgg cagatgattg gtcgaggaac
    31261 ccgtctatgt aaagatttat ttggacctga gcaggataag gaaaacttct tggtatttga
    31321 ttatggggac aattttgatt attttcgtgc agatccaaga gatggagagg gtcgtcacat
    31381 tgtttcgttg actcagcgtt tatttaatat caaagtggac ttgattcgag aacttcaggg
    31441 actccaatac caagaagatc agtttgcgag agcataccgt cagcagcttg tctcggaact
    31501 tcaaggtcgt atagagagct taaatgagtt ggacttcagg gttcgtatgg ttttagatac
    31561 agtttatagc tataggaaat tggaaagttg gcagaatcta actgctgtta caagtgaaac
    31621 cattcaaaaa aatctctctc tgcttttatt tgatgaagat aaagaagatg agatggcgag
    31681 gagatttgat ttgtggttgc ttcatattca gttggggcaa ctgacagcta aatcttccac
    31741 tgttcatatt tcccaagtga tgaagacggc tagagctctt tctgctattg gcaatatccc
    31801 gcaggttttt gagcaggctg aaattatcag gaaagtacag gagcctgaat tttggaaaga
    31861 agttaacttg tctgatttgg aaaaaattcg tcttgctatt cgagatttat tacagttttt
    31921 ggataaaaca gaccgtaaac cctactatgt taactttgaa gatcgtatac tctccactgt
    31981 tcacgagacc acagcatttt tgcaggtcaa cgatcttcgg tcttacaatg aaaaagttga
    32041 gcattatttg aaaactcatc tggatgagga gtccatttct aagctatacc ataataaaaa
    32101 gttgacatct gatgatatgc ttgcacttga aaaattgctt tgggaaaaat taggtagtaa
    32161 agcagactac caaagtcatt atgaaaataa ggcaattccg agattggttc gtgagattat
    32221 tggcttagat agagagtctg ccaatcgtat tttttctaaa tttttgtcgg atgagaatct
    32281 taatgccagg cagatttcat ttgtaaaatt gattgtagac tacattgtag aaaatggttt
    32341 tttagagacg aaagtgttaa cgcaagagcc gtttaaatct tatggttctg ttcaactact
    32401 cttccaacac caactaccag tacttcgtaa tattgttcaa atcattgaac ttatcaataa
    32461 tcgagctgga gaagcggctt aaattctaaa gtgattgcca tgctgagact catttaaaat
    32521 taaaaagagt agaaatttat gctatatatg agaagtttta ttaggaagaa tgtcatcgtt
    32581 ttcctagaat acagtatcag ttgttaagtg gttgataaat ttcaaagtag atacttgtac
    32641 cacgatgttt gttgatcgag ttattaacaa aagagctact ttgattttaa agaaatagaa
    32701 aacaaaaagc cgagcaagaa ttcaattgca ggagaaaatg aaataatact caatgaaaat
    32761 caaagagcaa actaggaaac tagctgcagg ctgctcaaaa cactgttttg aggttgtaga
    32821 tggaagctga cgcggattga agagattttc gaagagtata aatcttccta ggataaagca
    32881 aaacgcatag tatcaagggt tttcaacact tgatactatg cgttttctga tgttaaagac
    32941 tttctaccag gttttttaaa agcataattg ttagttgtag tcatttatta ttcttcaaag
    33001 aaaaatggtg gggcgaattt tttcagttct tcaaagcact tttgagcagt atctgcatct
    33061 tcacagatga taagacagac atcattacca caaagggtag cgatagcgtc agggaagctc
    33121 aaagtatcaa tgatagaacc aaaggattga gccagtccag gaagggtttt tagtaggact
    33181 tggtgttgaa ctgggcgcat ccagacaagg gcgtcttcca tgtagagttc gagacgtttt
    33241 tcccattttg agatggaacc attgttaaga acataataag cgctatcttc ttcgcggact
    33301 tttgataggt tcatattttt gatgtcgcgt gagagggttg cctgggttac ttgaatgtcg
    33361 ttctcagcaa gaagggcttg caactcagcc tgtgtatgaa tcttgttttt tgtgataaga
    33421 gcgcgtataa gttggtggcg gtgttctgat ttattcataa taatgtaact ccttttagca
    33481 aggtaaggta agcatggact gagcgaggtc gacagtcaag tggtagtctg tattgtcacg
    33541 gatggtgatt tcaaagtcag tagtatagag gactaaacgg agagtgtctc cttcttttag
    33601 cttgtaaata gttggctgca gttcaaattg aacgtccatc cattcatctg cagtaatatc
    33661 ctctactaac agtaaatcat ttctattttg taaattaagg taaccttttg tcacgactcg
    33721 ttgtgcctct ggtctaaatg gcaattcaca gagattttcc aacatgtgat agcgaccgtt
    33781 gtcaatggtt ctagcactta aaatagctgg ataaggttgt aggtatttct tttgcccaaa
    33841 ttctagcagt tgggcagata agagcccctt gtttgtactg gatttgatac gaagattgag
    33901 ctgagcgcga ccgtttaggt ggagatcttt agtcacagga aggttaatag taatctgatt
    33961 ggctttccct tgatagagct ctgtattgaa ggtttggtat gtcttaccat agcgctcaaa
    34021 atccttatct gggtactggt tttgaatagc ttgctcttct tgaccaagtg agaaggtttc
    34081 acagttttct tgcccaccga agttatcaag tgataaccaa gtctgtggag ctgtattgtc
    34141 ctgccagata acagtaggaa gttgaaagtc tgtttcctgt cctagtaatt tcttggtcaa
    34201 taaggcattt atggactcac ggaagtcaat tgattgccaa ttgttcatgt aaacatgggc
    34261 accattatgg aaaaagagat gcttgtgtat atgagtagga agagcatgga acatctggta
    34321 aacatgaagt ggtttgacat tccaatcctg agaaccatga gtaaagacaa cctctgcctt
    34381 tactttatgg gcattgagca gataattgcg gtcatgccaa aactgattgt agtccccagt
    34441 ttttcggtct agctgagctt tcactttttc taagtcagct tggtgagctt cattgccacg
    34501 gatatagtcg ccagctaaga gattacgaga ataggttaac tcagcaaggg agtcaaagtc
    34561 ctcacctgga taaccacctg ggctagtcac cagaccgttt tcacggtagt agttgtacca
    34621 tgatgaaatt cctgcctcgg caatgataac ttctaaacca tcgactcctg tagtcgcaag
    34681 accattggac atggtaccta gataggaaag tcctgttgta gcaacttttc cgtttgacca
    34741 atcagccttg acttgacgct ggcgcgtgtg atcagtaaag gcacggcaac ggccgttaag
    34801 ccaatcgatg acatttttat aagcctcgat ttgctggtag tctccattag tcatgaaacc
    34861 tgtcgagtct ttggtaccaa cacctgagac atagagattg gcaaagcctc tcggaaggaa
    34921 gtagtcgttt agtgtatagc tagagttgat gtgagttagc ttttcctcag cctctgctat
    34981 aagctcagct ttaccttggg gttggacgag atttagttga ggtttctcta gctcaatctt
    35041 gtgaggaagt ttaacctcaa gctcgccctc catcttgtag agagccttgt cactagcctt
    35101 gtcattggtt ccctgatgat aagggctggc tgtcatgatg gcagggattt ttccatcaaa
    35161 acgagggcga ataatgctaa cctttactag gtctgatagc cctttttggt cagtatcgac
    35221 acgagactca acgtaaacga cttcacgaat gacatcctgg ttagaaaaag tagccaaact
    35281 cttgccgtta aagtagtggt agtcattatc ctccggaata agaccatcac taacaagttg
    35341 gtcgataaga gtatttcctt ttttggtgcg agtattgagt aactgataga gattttcaat
    35401 caagtcacca tatataatgg gaaatccagt ttctttacga aaaacgtcac tatcttcgaa
    35461 gtcaaccaaa taagaaaagc ctaaaagttg aaaagcaaca gtataaaaaa tatctgctgt
    35521 cagttcatct tctgattgaa aaaatgtcag caggtctgtt tttttatcag ctgctaggat
    35581 agaaagtggg tagttggtgt cttgataagt gaaaaagaaa cgacgtaaaa aggtttcaag
    35641 tgagtctttg tgattggctg tattttgtaa atcaaagcca cattttttta gttcagataa
    35701 gacattttct tttggaaaat tgatataact atattgatta aaacgcatag aacctccata
    35761 tagaatgaca gttaaggtta ttatatcaaa aaaaaagcag aaagggaatt gttaacttca
    35821 aaaggaaata atccaataaa aatgaataaa gtactaaatt caatatagag aacagagtaa
    35881 caataagaat aaatagatag ggtataaaag ttctaggaga tttatattat atgctttcta
    35941 tttttatata caatatagta taaatataaa aatgatgaca aaaatacaaa tgaatagaaa
    36001 ataaattagt aagctgatga aatttttctc aagagaagcc atttataggt gaaaatggta
    36061 taatatagtg agaaggatag aggagaagtg taaattgatc gcacaactag atacaaaaac
    36121 agtctatagt tttatggaaa gcgtcatttc gatcgaaaag tatgtgagag cagctaaaga
    36181 atacggctac actcatttgg ctatgatgga tattgacaat ctttatggcg ctttcgactt
    36241 tctagagatt acaaaaaaat acggcattca tcctttgcta gggcttgaaa tgacagtgtt
    36301 tgtagatgat caggaagtga atttgcgctt tttagctcta tctagtgtgg gctatcagca
    36361 gttgatgaag ctttcgacag ccaagatgca gggggagaaa acttggtcag tcctgtccca
    36421 gtacctggag gatatcgcgg tcattgtgtc ttattttgat agagttgagt cgttagaact
    36481 aggctgtgat tactatatag gggtttatcc agaaacacta gcaagcgaat ttcatcatcc
    36541 tatcttacct ctttatcggg tcaacgcttt tgaaagcagg gatagagaag ttcttcaagt
    36601 tttaacagcg attaaagaaa atctaccgct cagagaagtt cccttgcgtt cgagacaaga
    36661 tgtctttata tcagcaagtt ctttagagaa actattccaa gagcgttttc cgcaagcttt
    36721 ggacaattta gaaaagctta tttcaggcat ttcttacgac ttggatacta gtctgaaact
    36781 gcctcgtttt aatccagcta gaccagcagt agaggagttg agagagcgtg ctgaactggg
    36841 gcttgttcag aaggggttga ctagtaaaga atatcaagat agactagacc aagaattgtc
    36901 tgttattcat gatatgggct ttgatgatta tttcttggtt gtttgggatt tgttgcgttt
    36961 tggacgatcg aatggctatt atatgggaat gggaaggggt tctgcagtag gcagtttggt
    37021 ttcttatgcc ttagacatca cggggattga cccagtagag aaaaatctga tttttgaacg
    37081 ctttcttaat cgtgaacgct ataccatgcc tgatattgat attgatatcc cagatattta
    37141 tcgtccagat tttatcagat atgttggtaa taaatatggt agtaaacatg cggcacaaat
    37201 cgttactttt tcaacctttg gagccaagca agctcttcga gatgtcttga aacgctttgg
    37261 tgtgccagag tatgaattat ctgcaattac taagaaaatc agttttcgtg acaatcttaa
    37321 gtcggcctat gagggcaatc tccagtttcg tcagcaaatc aatagtaagt tagaatacca
    37381 aaaagctttt gagattgctt gcaagataga gggctatcca aggcaaacct ctgtccatgc
    37441 ggctggtgtt gtaattagtg accaagattt aaccaactac attcctctaa agtatggtga
    37501 tgaaattcca ctgactcagt atgatgctca tggagttgag gctagcggac ttttgaagat
    37561 ggactttctg ggactacgaa atttgacctt tgtccagaag atgcaagagt tgcttgctga
    37621 aacagaaggt attcatctga aaattgaaga aatcgattta gaagacaaag aaacgttagc
    37681 tttatttgcc tctggtaata caaaaggtat ctttcaattt gagcaaccag gtgccattcg
    37741 tctgcttaag cgtgtgcaac cagtctgttt tgaagatgtc gtcgcgacta cttctctaaa
    37801 tcgaccaggt gcaagtgact acatcaataa ttttgtggca aggaagcatg ggcaggaaga
    37861 agtgactgtt ctagattcag ctctggagga tattttggct ccaacttatg gcattatgct
    37921 ctatcaggag caggttatgc aggttgctca gcgatttgct ggatttagtc ttgggaaggc
    37981 cgatattttg cgtcgagcta tgggtaaaaa ggatgcctct gctatgcatg agatgagggc
    38041 ttcctttatt caaggctcca tagaagcagg ccatactgcg gaaaaagcag agcaggtttt
    38101 tgatgttatg gagaagtttg caggttatgg atttaataga tctcatgcct acgcctactc
    38161 agccttggcc ttccagttgg cttatttcaa aacgcattat ccagccattt tttatcaggt
    38221 catgttaaat tattccaaca gtgattactt aatagatgca cttgaagcag gttttgaagt
    38281 agcctctcta tccatcaata ctattcccta tcacgataaa attgccaaca agtctatcta
    38341 tataggtttg aaatcgatta aggggctcag caaggacttg gcgctttgga ttattgaaca
    38401 tagaccttat tctaacattg aagattttat agctaaatta cctgagaatt atctgaaact
    38461 tcctctgcta gaacctttgg taaaagttgg tcttttcgat tcatttgaaa aaaatcgtca
    38521 aaaagtattt aataacttag ctaatctatt tgaatttgtg aaagagttgg gaagtttgtt
    38581 tggagatgct atttatagtt ggcaggaatc ggaagattgg acggaacaag aaaaatttta
    38641 tatggaacaa gagcttttag ggataggtgt cagcaaacat ccactacaag ctattgcaag
    38701 taaggctatt tacccgatta ccccaatcgg aaatttgtca gaaaatagct atgctattat
    38761 tttggttgaa gttcagaaaa taaaagtgat tcgtaccaaa aagggtgaaa atatggcctt
    38821 cttacaggca gatgatagta agaaaaaatt ggatgtcact ctcttttcag acttatatcg
    38881 tcaggttgga caggaaataa aagagggagc cttctactat gtaaaaggaa aaatacaatc
    38941 acgtgatggc cgtctgcaaa tgattgcaca agaaataaga gaagcagttg ctgaacgctt
    39001 ttggatacag gtgaaaaatc atgaatcgga tcaagaaatt tcacgcattt tagaacaatt
    39061 taaaggccca atcccagtca tcatccggta tgaagaggaa cagaaaacca tcgtttctcc
    39121 ccatcatttt gtagctaaat ccaatgaatt agaggagaaa ttgaatgaaa tcgttatgaa
    39181 aacgatttat cgctaaaaat acggaaaata gaagaatttt aaaatcaaat gtgatataat
    39241 caagaagaat gttaaaagaa aaaggagcat aaaccaatat gaaacgtatt gctgttttga
    39301 ctagtggcgg agacgcacct ggtatgaacg ctgccatccg tgcagttgtt cgtcaagcaa
    39361 tttcagaagg aatggaagtg tttggtatct atgacggata tgctggtatg gttgccggtg
    39421 aaattcatcc cctagatgca gcttcagtag gggacatcat ttctcgtggt ggtacttttc
    39481 ttcactcagc tcgttatcca gagttcgctc aacttgaagg gcaacttaaa gggattgagc
    39541 aattgaaaaa acacggaatt gaaggtgtag ttgttatcgg tggtgacgga tcttaccacg
    39601 gcgctatgcg tttgactgaa catggcttcc cagctattgg tcttccaggt acaatcgata
    39661 acgatatcgt tggtactgac tttacaatcg gttttgacac agcggttact actgccatgg
    39721 acgctatcga taagattcgt gatacatcat caagtcaccg tcgtactttt gtaatcgaag
    39781 ttatgggacg taacgctggt gatatcgctc tttgggctgg tattgcaact ggtgctgatg
    39841 aaatcatcat ccctgaagca ggcttcaaga tggaagatat cgtagcaagc atcaaagctg
    39901 gttatgaatg tggtaaaaaa cacaatatca tcgtcttagc tgaaggtgtg atgtcagcgg
    39961 ctgaatttgg tcaaaaactt aaagaagctg gagatacaag cgaccttcgt gtaacagaac
    40021 ttggacatat tcaacgtggt ggttctccaa ctgcgcgtga ccgtgttttg gcgtcacgta
    40081 tgggtgcaca tgctgttaaa cttcttaaag aaggtatcgg tggtgttgcg gttggtattc
    40141 gtaacgaaaa aatggttgaa aatccaattc ttggtactgc agaagaaggg gcattgttta
    40201 gccttactgc agaaggtaag attgtggtta acaacccgca caaagccgat attgagctat
    40261 ctagcttgaa taagagcttg tcataattta caatttagtt aataagacca aaaaggtcat
    40321 atatataaag gagtcacaaa aatcatgaac aaacgtgtaa agatcgttgc aactttgggt
    40381 cctgcggtag aaatccgtgg tggtaaaaaa ttcggtgagg acggatactg gggtgaaaaa
    40441 cttgatgttg aagcttcagc taaaaacatt gctaaattga ttgaagctgg tgctaacaca
    40501 ttccgattca acttctcaca cggcgaccac caagaacaag gtgagcgtat ggcaactgtt
    40561 aaacttgcgg aaaaaattgc aggtaaaaaa gttggtttcc ttcttgatac aaaaggacct
    40621 gaaatccgta cagaattgtt cgaaggtgaa gctaaagaat attcatacaa aactggtgaa
    40681 aaaattcgtg ttgcaactaa acaaggaatc aaatcaactc gtgaagtgat tgcgttgaac
    40741 gttgctggtg ctcttgatat ctatgatgat gttgaagttg gtcgtcaagt tttggttgac
    40801 gatggtaaac ttggtcttcg tgtggttgct aaagatgatg caactcgtga atttgaagtt
    40861 gaagttgaaa acgatggtat catcgctaaa caaaaaggtg tgaacatccc taacactaaa
    40921 attcctttcc cagctcttgc tgaacgcgat aacgacgata tccgtttcgg tcttgaacaa
    40981 ggtatcaact tcatcgcaat ttcattcgta cgtactgcaa aagatgtgaa cgaagttcgt
    41041 gcaatctgtg aagaaactgg aaacggacat gttcaattgt tcgctaaaat cgaaaaccaa
    41101 caaggtatcg ataacttaga tgaaatcatc gaagcagctg atggtattat gattgctcgt
    41161 ggtgatatgg gtatcgaagt accgttcgaa atggttccag tttatcaaaa aatgattatc
    41221 aagaaagtca atgctgcagg taaagttgtt atcactgcaa caaacatgct tgaaacaatg
    41281 actgaaaaac cacgtgcaac tcgttcagaa gtatcagacg tattcaacgc tgttatcgac
    41341 ggaactgacg ctacaatgtt gtcaggcgag tctgcaaacg gtaaataccc actcgagtca
    41401 gtaactacaa tggctacaat cgacaagaac gctcaagctc ttcttaatga atacggacgt
    41461 cttgattcag attcatttga gcgtaactct aagacagaag taatggcttc tgctgttaaa
    41521 gatgctacta gctcaatgga tatcaaattg gttgtaactc ttactaagac aggtcatact
    41581 gcacgtttga tttctaaata ccgtccaaat gctgacatct tagcattgac atttgacgaa
    41641 ttgacagaac gtggcttgat gttgaactgg ggtgttatcc caatgttgac agatgctcca
    41701 tcttcaactg acgatatgtt cgaaatcgct gaacgtaaag cggtagaagc aggtctcgtt
    41761 gagtcaggcg atgatatcgt tatcgttgct ggtgtgccag taggagaagc tgttcgcaca
    41821 aacacaatgc gtatccgcac agtacgttaa gaaaaatata aaaacctatc atatccagct
    41881 ttctagcctg tatgatgggc tctttttttg tccagggggc aaaaaggggg caaaactttc
    41941 ataatggatt gatactcttc gaaaatcaaa ttcaaaccac gtcagcgtcg ccttaccgta
    42001 tatatgttac tgacttcgtc agttctatct acaacctcaa agcagtgctt tgagcaacct
    42061 gcggctagct tcctagtttg ctctttgatt ttcattgagt atgaaataag atatgcataa
    42121 attgattaga aagtcaaatg aatttctaca aatgttttag caatcgcaat ctacttgtct
    42181 agattcgatc tgatatattt tcgatttaat gatatggtat ttaaaacctc caaagtagct
    42241 tgctccattc ttttacctac gtgagtctag atgttattta ctgttttagc gtttttgtgt
    42301 tctactctaa ccattatagc attcttctca gctagtgtac taaggagtgt gtgcctgaaa
    42361 atatgggaac taaggggctg gtttatcggt ttctctagtt tagtatttgc cttttgcaaa
    42421 gtgatcttaa atgcctttct ctaaatttac atatcactat tgtttaacaa aatctaatct
    42481 attttaggtc acttattctt tttttgaaat gtagaatgaa ctttttcaaa gtttttcgaa
    42541 tcttttaaaa tctgtttgtt ttatatcgcc attctccccc ttttttaatt ctccctatat
    42601 agcctgacaa cttttccgat ggtacgaata tggttgcttt cgtctaggtg gatgtcggag
    42661 tattcgggat tgagtttttt tgaggtagcc ttggcggagt ttcttgacat agttagtgcc
    42721 gtctacttgg aagatgccga tggtattata gtcaatctgt ggggtattct tgataaatag
    42781 gtagtcgctg tttcttatct ttggctccat ggacttgctg acgacataag cgattgggtc
    42841 gtagtcgtct gggataatgg aaactccata actaaatcgt cgtcctgcat caagcggcta
    42901 cctgcagaga taaactacct aacacgagag taagtagtct gtctgtagtc gtccagtctg
    42961 atgattttta cgatacttcg tttttctgat catacagttg cctctcggca taggtcagaa
    43021 ctttaccttg tctgggtggt gcccgttggt cgtagataga ttggatatcg ctaggagaat
    43081 ccttttgaac tggaggaaag agggcatcaa tcaagctact gaatacttta actaagtcaa
    43141 atatagtatt tttcttagta gacctaaccc ttttttcata atttctaatg gtgtttttac
    43201 ttatacctat cttagtaccc aattcttatt gagtccaacc attactagtc tatattgttt
    43261 tatagttgat tgagtttgga atagtacgct gtagctgcta aaacatttct aggaattaat
    43321 ttgcctttcc taatagagtt gttcatatct tatttcaatc tactatattt ttcacctcta
    43381 acaatcgcaa tctcttcttt atccatgaac gaaaacgctt tctatctttg taagtaaagc
    43441 atcacacgaa atccacgaaa atgaaaacct ttgttgtgtt ttcgtaaaaa atttgttgac
    43501 agagcacgaa acgcctacta taattaaatc aagtttaagg aaataacaaa aaaacaaatc
    43561 ggagggaaag acgatgaaca cattaaatga gaaagtaatc aatatctgta aagcagtagt
    43621 taaagaaact ttaatccaag acatttagga gaaagaaatg aagaaatcaa gaaaattagc
    43681 cacattagga atctgttcag ccttgttttt aggcttggct gcttgtcaac aacaacatgc
    43741 tacttctgag gggacgaatc aaaggcaaag cagttcagcg aaagttccat ggaaagcttc
    43801 atacaccaac ctaaacaacc aggtaagtac agaagaggtc aaatctctct tatcagctca
    43861 cttggatcca aatagtgttg atgcattttt taatctcgtt aatgactata ataccattgt
    43921 cggctcaact ggcttatcag gagatttcac ttcctttact cacaccgaat acgatgttga
    43981 gaaaatcagt catctctgga atcaaaagaa gggcgatttc gttgggacca actgccgtat
    44041 caatagttat tgtcttttga aaaattcagt caccattcca aagcttgaaa agaatgacca
    44101 gttgcttttc ctagataatg atgcgattga taaaggaaag gtctttgatt cacaagataa
    44161 ggaagagttt gatattctat tttcgagagt tccaactgag gcaactacag atgtcaaggt
    44221 tcacgctgaa aagatggaaa cattcttctc acaatttcaa ttcaatgaaa aagctcgaat
    44281 gctgtctgta gtcttgcacg acaatctgga tggcgagtat ctgtttgtag gccacgttgg
    44341 ggtcttagta cctgctgatg acggtttctt atttgtagag aaattgactt tcgaagagcc
    44401 ctaccaagcg attaaatttg ctagtaagga agattgctac aagtatttag gcaccaagta
    44461 tgcggattat acaggcgagg gactggctaa gccttttatc atggataatg ataagtgggt
    44521 taaactttaa tagtgttatg atatgacagt aatcttagga tttttatttc tatttttgat
    44581 gagttggtgg gttatttcaa tcgttaatag ccagaaacca cgcaaaaatg aaacatttat
    44641 tggctatatc caaagatacg atattgatgg gaatggctat caatcattac cgtgtcctca
    44701 gaactgagag gagttcttga aatcgtaaaa ctactttgaa caagagttac ttcaacccat
    44761 tggctccgac ggattaagtt gctttcgtga acaccaaaat cagccgcaat ttcttcataa
    44821 gtgcggtatt ctcgcacata ttgaagagtg gccataagaa ggtcttctag gcttaattta
    44881 ggttttcgtc ccccttttgt gtgtttaagt tgataagcag tttttaatac agctaacatc
    44941 tcttcaaaag tggtgcgctg aacaccaaca agacatttaa atcgtgcatc agttagttgc
    45001 ttacttgctt cataattcat agaactacta taccatattt tgtttcgcag gaagtctaat
    45061 gctaaaatct tatcagtgag aagatttctt aaagtttatt ctttgtagtt tcatctgtat
    45121 tagtacataa tttaacttag agaaaatagg aggattaatc gaatggaatt aaaagatttt
    45181 acagaaaaag aacaggaaat gattaagaaa aggcttacaa tgtctaatat tagtgacaaa
    45241 gaaactactg agaagattct ggcgctagta ccacaagact tgattaagcg aatcccgttt
    45301 tttgtcagaa aacatgctac aacacgtacg attaaacgca tttcaattga acaccctgaa
    45361 ctctacgctg tagctcaaac aagtggtgag attccagaaa aaaaaacgcg aagaattgcg
    45421 tcagattatc acaactatct ttgaacaaaa gatgaataag catagtatta agtagagaat
    45481 gaaaaaatat tttattggcg gtttgggaag caatgcctat catagcaagg attttcttta
    45541 agaactagat tcgcaggtct attttctaaa tccatatgaa aagcatcttc gagatgaaac
    45601 agaattgaaa tcatggttta aaaatgagat tgtagaggaa gaatctatct gtctgatagg
    45661 tcattctatt ggaggcgatt tagctcgtta tttcgcatcg gaatttgaag aagtaaaga
//
LOCUS       DBERYS010000012        45210 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.012, whole genome shotgun sequence.
ACCESSION   DBERYS010000012 DBERYS010000000
VERSION     DBERYS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 45210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.012"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1593)
                     /locus_tag="ACL8LA_000751"
     CDS             complement(<1..1593)
                     /locus_tag="ACL8LA_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGS1812549.1"
                     /translation="MMTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEK
                     GRDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLITFWDNQHYVVIYKVKKNK
                     VYIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKG
                     RLILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILAR
                     SMGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVV
                     GGVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSL
                     IYSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQL
                     FAPIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYTQYILAAGYLNRV
                     NDIWLENEENVENGLKKCSLEGRIDIKDLSFSYSKDSAPVIENLNLTIEPGQRIALVG
                     QSGSGKSTLSKILSGLYKIDTGK"
     gene            complement(1583..2131)
                     /locus_tag="ACL8LA_000752"
     CDS             complement(1583..2131)
                     /locus_tag="ACL8LA_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS1812550.1"
                     /translation="MSLSYYYEINPSTDILKCIEELLYKEDKCFNNILKNWKDIRRNH
                     NDSFPNFWCYGAPGILLARKEIFDKTNIGNNDLSIIKNVLTNVEKIRELNLCHGSVGT
                     ISCLDAILKDEENLLIKESIDFYFDNVVSQVIKPELSTDLNTMNTFSFMLGVSGVVYE
                     ISRKQDDRLLNVLLLELRGHDD"
     gene            complement(2212..2274)
                     /locus_tag="ACL8LA_000753"
     CDS             complement(2212..2274)
                     /locus_tag="ACL8LA_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HGS1812551.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            2354..3208
                     /locus_tag="ACL8LA_000754"
     CDS             2354..3208
                     /locus_tag="ACL8LA_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1812552.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKIEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGENKLKLHYTDH
                     FNKLVNKK"
     gene            complement(3253..5138)
                     /locus_tag="ACL8LA_000755"
                     /pseudo
     CDS             complement(3253..5138)
                     /locus_tag="ACL8LA_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775543.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
     gene            complement(5161..5490)
                     /gene="mobC"
                     /locus_tag="ACL8LA_000756"
     CDS             complement(5161..5490)
                     /gene="mobC"
                     /locus_tag="ACL8LA_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HGS1812553.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            complement(5500..5859)
                     /locus_tag="ACL8LA_000757"
     CDS             complement(5500..5859)
                     /locus_tag="ACL8LA_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HGS1812554.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            complement(6691..7068)
                     /locus_tag="ACL8LA_000758"
     CDS             complement(6691..7068)
                     /locus_tag="ACL8LA_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812555.1"
                     /translation="MGQKVVAMLDFLLAYSDYSKDFRPLIIDQPEDNLDNRYIYRHLV
                     QQFRDVKAQRQIILATHNATIVTNSMTDQVVIMESDGVNGWIESQGYVSEKYIKNHII
                     NQLEGGKDSFKHKMSIYETALSE"
     gene            complement(7071..7847)
                     /locus_tag="ACL8LA_000759"
     CDS             complement(7071..7847)
                     /locus_tag="ACL8LA_000759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812556.1"
                     /translation="MRGFNNKIKSVYQELTNSKEKFGSFHKTLIHLHTPVSYDYKLFS
                     NWTATKYRKITEDELYDIFFENKKIKVDKTIFFSNFDKVVFSSSKEYISFLMLAEAII
                     KNGIEIVVVTDHNTTKGIKKLQMAVSIIMKNYPIYDIHPHILHGVEISAADKLHIVCI
                     YDYEQESWVNQWLSENIISEKDGSYQHSLTIMKDFNNQKIVNYIAHFNSYDILKKGSH
                     LSGAYKRKIFSKENTRFWSLILTRKNFRNNLIFSIKKLVY"
     gene            complement(7875..8636)
                     /gene="pezT"
                     /locus_tag="ACL8LA_000760"
     CDS             complement(7875..8636)
                     /gene="pezT"
                     /locus_tag="ACL8LA_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HGS1812557.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELFFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(8636..9112)
                     /gene="pezA"
                     /locus_tag="ACL8LA_000761"
     CDS             complement(8636..9112)
                     /gene="pezA"
                     /locus_tag="ACL8LA_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HGS1812558.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(9557..10012)
                     /locus_tag="ACL8LA_000762"
                     /pseudo
     CDS             complement(9557..10012)
                     /locus_tag="ACL8LA_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10287..12026)
                     /locus_tag="ACL8LA_000763"
     CDS             complement(10287..12026)
                     /locus_tag="ACL8LA_000763"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGS1812559.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESASNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELD
                     NQLVISPDGFMIF"
     gene            complement(12189..13073)
                     /locus_tag="ACL8LA_000764"
     CDS             complement(12189..13073)
                     /locus_tag="ACL8LA_000764"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS1812560.1"
                     /translation="MKKAMVIITPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEAIHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKLKED"
     gene            complement(13185..14039)
                     /locus_tag="ACL8LA_000765"
     CDS             complement(13185..14039)
                     /locus_tag="ACL8LA_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGS1812561.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(14097..14855)
                     /locus_tag="ACL8LA_000766"
     CDS             complement(14097..14855)
                     /locus_tag="ACL8LA_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS1812562.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(14917..15105)
                     /locus_tag="ACL8LA_000767"
     CDS             complement(14917..15105)
                     /locus_tag="ACL8LA_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGS1812563.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(15427..15993)
                     /locus_tag="ACL8LA_000768"
     CDS             complement(15427..15993)
                     /locus_tag="ACL8LA_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812564.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(16261..17940)
                     /locus_tag="ACL8LA_000769"
                     /pseudo
     CDS             complement(16261..17940)
                     /locus_tag="ACL8LA_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(18032..18193)
                     /locus_tag="ACL8LA_000770"
     CDS             complement(18032..18193)
                     /locus_tag="ACL8LA_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812565.1"
                     /translation="MNNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(18877..19176)
                     /locus_tag="ACL8LA_000771"
                     /pseudo
     CDS             complement(18877..19176)
                     /locus_tag="ACL8LA_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19157..19546)
                     /locus_tag="ACL8LA_000772"
     CDS             complement(19157..19546)
                     /locus_tag="ACL8LA_000772"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1812566.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(19599..19783)
                     /locus_tag="ACL8LA_000773"
                     /pseudo
     CDS             complement(19599..19783)
                     /locus_tag="ACL8LA_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612340.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(19934..20728)
                     /locus_tag="ACL8LA_000774"
     CDS             complement(19934..20728)
                     /locus_tag="ACL8LA_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812567.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
     gene            complement(20728..21735)
                     /locus_tag="ACL8LA_000775"
     CDS             complement(20728..21735)
                     /locus_tag="ACL8LA_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGS1812568.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(21735..22742)
                     /locus_tag="ACL8LA_000776"
     CDS             complement(21735..22742)
                     /locus_tag="ACL8LA_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGS1812569.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(22732..23757)
                     /locus_tag="ACL8LA_000777"
     CDS             complement(22732..23757)
                     /locus_tag="ACL8LA_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS1812570.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            complement(24150..24368)
                     /locus_tag="ACL8LA_000778"
     CDS             complement(24150..24368)
                     /locus_tag="ACL8LA_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1812571.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(24614..24892)
                     /locus_tag="ACL8LA_000779"
     CDS             complement(24614..24892)
                     /locus_tag="ACL8LA_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812572.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(25106..26737)
                     /gene="rlmD"
                     /locus_tag="ACL8LA_000780"
     CDS             complement(25106..26737)
                     /gene="rlmD"
                     /locus_tag="ACL8LA_000780"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS1812573.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLDMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(26811..27785)
                     /locus_tag="ACL8LA_000781"
     CDS             complement(26811..27785)
                     /locus_tag="ACL8LA_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGS1812574.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(27859..28473)
                     /gene="pmp23"
                     /locus_tag="ACL8LA_000782"
     CDS             complement(27859..28473)
                     /gene="pmp23"
                     /locus_tag="ACL8LA_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGS1812575.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(28473..29447)
                     /locus_tag="ACL8LA_000783"
     CDS             complement(28473..29447)
                     /locus_tag="ACL8LA_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGS1812576.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(29455..30711)
                     /gene="glyA"
                     /locus_tag="ACL8LA_000784"
     CDS             complement(29455..30711)
                     /gene="glyA"
                     /locus_tag="ACL8LA_000784"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGS1812577.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQTN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(30773..31204)
                     /locus_tag="ACL8LA_000785"
     CDS             complement(30773..31204)
                     /locus_tag="ACL8LA_000785"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812578.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(31206..31808)
                     /locus_tag="ACL8LA_000786"
     CDS             complement(31206..31808)
                     /locus_tag="ACL8LA_000786"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGS1812579.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(31792..32631)
                     /gene="prmC"
                     /locus_tag="ACL8LA_000787"
     CDS             complement(31792..32631)
                     /gene="prmC"
                     /locus_tag="ACL8LA_000787"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGS1812580.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(32631..33710)
                     /gene="prfA"
                     /locus_tag="ACL8LA_000788"
     CDS             complement(32631..33710)
                     /gene="prfA"
                     /locus_tag="ACL8LA_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGS1812581.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(33707..34297)
                     /locus_tag="ACL8LA_000789"
     CDS             complement(33707..34297)
                     /locus_tag="ACL8LA_000789"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGS1812582.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            34420..34602
                     /locus_tag="ACL8LA_000790"
     CDS             34420..34602
                     /locus_tag="ACL8LA_000790"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGS1812583.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            34738..36111
                     /gene="mnmE"
                     /locus_tag="ACL8LA_000791"
     CDS             34738..36111
                     /gene="mnmE"
                     /locus_tag="ACL8LA_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGS1812584.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(36158..37093)
                     /gene="dapA"
                     /locus_tag="ACL8LA_000792"
     CDS             complement(36158..37093)
                     /gene="dapA"
                     /locus_tag="ACL8LA_000792"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGS1812585.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(37149..38225)
                     /locus_tag="ACL8LA_000793"
     CDS             complement(37149..38225)
                     /locus_tag="ACL8LA_000793"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGS1812586.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(38766..38838)
                     /locus_tag="ACL8LA_000794"
     tRNA            complement(38766..38838)
                     /locus_tag="ACL8LA_000794"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38803..38805),aa:Thr,seq:ggt)
     gene            complement(38938..39447)
                     /locus_tag="ACL8LA_000795"
     CDS             complement(38938..39447)
                     /locus_tag="ACL8LA_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGS1812587.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(39445..39541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            39570..40001
                     /locus_tag="ACL8LA_000796"
     CDS             39570..40001
                     /locus_tag="ACL8LA_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGS1812588.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            40083..40460
                     /gene="mscL"
                     /locus_tag="ACL8LA_000797"
     CDS             40083..40460
                     /gene="mscL"
                     /locus_tag="ACL8LA_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGS1812589.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(40502..41596)
                     /gene="hemH"
                     /locus_tag="ACL8LA_000798"
     CDS             complement(40502..41596)
                     /gene="hemH"
                     /locus_tag="ACL8LA_000798"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGS1812590.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            41751..42974
                     /gene="pepT"
                     /locus_tag="ACL8LA_000799"
     CDS             41751..42974
                     /gene="pepT"
                     /locus_tag="ACL8LA_000799"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGS1812591.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(43010..43663)
                     /locus_tag="ACL8LA_000800"
                     /pseudo
     CDS             complement(43010..43663)
                     /locus_tag="ACL8LA_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            43661..43825
                     /locus_tag="ACL8LA_000801"
                     /pseudo
     CDS             43661..43825
                     /locus_tag="ACL8LA_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(44096..45107)
                     /locus_tag="ACL8LA_000802"
                     /pseudo
     CDS             complement(44096..45107)
                     /locus_tag="ACL8LA_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1,RefSeq:WP_007521835.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT        13566 a         8977 c         7217 g        15450 t
ORIGIN      
        1 ttttcctgta tcaatcttat ataatcctga tagtattttt gataacgtac tttttcctga
       61 ccccgattga ccgactagtg caattctctg tcctggctca atagtcaaat ttaaattttc
      121 tattacagga gctgagtctt tggaataaga gaaagatagg tccttaatat ctattctacc
      181 ttctaaagaa cactttttaa gaccattttc aacattctct tcattttcaa gccagatatc
      241 atttactcta tttaagtatc ctgctgccaa aatatactgc gtataagcat taaatattga
      301 gatttctgaa ctaaataaaa ttgatgccat ggtttgaaat gcgacaacct cacccaataa
      361 aatttttcca tttaaaacat tatcaagtcc aaaaatcaat ataattatag gagcgaacaa
      421 ttgaaataca ttagtagctg aattgtataa aatatgcaca atacttcgtt ttttatactt
      481 tgtcaatacg ttttttaaat acttagacca gttttcatat atttcatctt ctaatcccga
      541 tatttttatt gaagaaatag tatttaatgc ttctgtttct actgagtaaa tcaatgattg
      601 ttcttgtaat tctctattaa ccgtatcata cattattttt ctagtactaa acagaaacaa
      661 aaaattgatt aaactcaata taagagctat aattgataat gtttttgaac taagaaacat
      721 atatattaca acacttagaa tagtcccgat atctactact cccccaacaa cttggttagt
      781 aaatagctcc ctaaaaccac taagactaga aattctgtat aaaatatctc cttttgccct
      841 gagttcaaaa aaactatatg gtagtttaag caaatgctta aatgtaaaac tttcaatatc
      901 tctactaaaa aatatattag aaaatagaat tcccatactc ctagccaaga tagaaataag
      961 atacaaacaa agtagagaaa ataaaattaa aaacgaggtt tgaaaagaaa atgtattccc
     1021 taatgcagaa ttaataaact ttgaagtcat tactggtacg gaaagtatta ttaagtaact
     1081 tattattgaa aatagtaacg tcaaaatcaa tcgacctttt acctttgaaa aactagagaa
     1141 tactcttatc catggagaag gaaactgcga cttcaaagat tgataatttt cgttaggaaa
     1201 tgatagcagt actatattac taaaatgttt tgaaaactcc ttaaactcat aatttatata
     1261 tcctttagaa ggatccatta tatagacctt attctttttt actttgtaaa taactacata
     1321 atgttgatta tcccagaatg ttattaatgg taacggaagt tcactaattt tatttacatc
     1381 tttaacttga tatgagccag tatcaaaatt tcgtttattc aataactgtt tcaagtcacc
     1441 tatactataa ccatctctcc ctttttctat atcacgtctt aaatctagta atgtctcttt
     1501 gcttttatag aaatgtagta tggacaagca gcaacataaa ccacattcta tttgagacat
     1561 ttgctcaata ttaggtactt ttctagtcat catgtcctct taactccagt aataaaacat
     1621 taagtaatct atcatcttgt tttcgagata tttcatagac aactccagat actcctagca
     1681 taaaagaaaa tgtattcata gtattcaaat ccgtacttaa ttctggtttg atgacctgag
     1741 aaacgacatt atcaaaataa aaatctatag attcttttat taataggttt tcttcatctt
     1801 tcaatattgc atcaagacaa ctgatagtac ctacagaacc gtgacataaa ttcaattctc
     1861 ttattttttc tacattagtt aatacatttt taattattga taaatcatta ttcccaatat
     1921 tagttttatc gaaaatttct tttctagcta ataaaatgcc cggagctcca taacaccaaa
     1981 aattaggaaa ggaatcatta tgatttcttc ttatgtcttt ccaatttttt aatatattat
     2041 tgaaacattt atcttcttta tatagtaatt cctctataca ttttaatata tctgtactag
     2101 gattaatttc ataataatat gataatgaca ttgctactcc agaataaccg tgtgctaaac
     2161 ctgttactgt tatattatct aattgtgaaa tacaataatt aatacacttt tttaaatgat
     2221 gataatatcc atcaccaaaa ccaaaatcgg taaaaatttt gaaattttct tcatacttgg
     2281 acttcctttc cgtttttatt tattgtacaa taaataaaaa cggatttaat ttttgttggg
     2341 aaaatgggaa attatgaaat caaaacttgg catcacactt agaaaagttc gtaaaggaaa
     2401 acaaattagt ctatgctctg tagcagatga acatctttca aaatcacaaa tatctcgttt
     2461 tgaacgtggg gagtcagaga tatcttgtat tcgactaatc aatattttag acaaattaca
     2521 tattactttg gatgagttcc ttgttttaca caatgacgac tataccagtt ccgaatcatt
     2581 tgctaatcta gtacagtaca ttcgtaagca atattcatca caaagtatca ataatatagc
     2641 atgtctactt tctgatactt ctgattacac tctaaattct tttgagaaaa caatggtaaa
     2701 atctatttta cacacaatgg attcgaacat cattccatca gatgaagagc ttcttcatct
     2761 gacagactat cttttcaaaa ttgaaaaatg gggctattat gaaatcattt tattaggtaa
     2821 ctgtgtacgc acaattaact acaactccta cttcttatta actaaagaga tgttaaataa
     2881 ctatatttac tcatctctga ataaaactaa taagcagatt gtctctcagc ttgctattaa
     2941 ttgcttcatt ctaagcattg ataaggaaga attttctaat tgctcctatt taattagtaa
     3001 gataaagaca ttgttggata atgaactaaa cttttatgaa caaacagttt ttctctatgc
     3061 aactggttac tttgaattta aacgttgcca atcaaccagt ggaattgaaa aaatgaaaca
     3121 agccatacaa gtcttcgata ttttaggaga aaataagtta aaattacact atactgacca
     3181 ttttaataaa ctagtaaaca aaaaataaat gaaattattc tctatctttt aactaattag
     3241 tcgtaaataa tattattcta gtaaggcatt ctcgtgaaat tttagaactg taaatttatt
     3301 ggtattaata attttcacaa acaaatccaa tcttctagct atctcctcat atttattaat
     3361 ttcattacta agttcctgtt gtaattttaa aatatatgta ttgagtcgat ctaacattat
     3421 tgattccgtc atgttcattt ggtcaaaatc atacttagct aattttctac cgatctcatc
     3481 ttcactcttc aaattgataa ggacttccgc catcttgttt aaggtggcga ttttttcttg
     3541 ggtttcttct agcttcatat caatctctgc aatttctaat acaagctcat ctttgatttc
     3601 ttggtattgt ttgttcgtgt tagatagttc aatcataaga ttaatttcag aaatcttctc
     3661 ttgaagactt tttaaagttg gtcgtctata ggggagtttt tggctatcgt tgctcaattg
     3721 tctgattaag gtcctaccct tgatgaaaca attattatcc atgttttctt tattatagac
     3781 aaagtaagaa gacgtttctc gaatgtagac tttatatttt ttatgatttt cttcttccat
     3841 aatatccaat tgatagttgg gaatgaaaat aagaccgctc tatttgacac cgaaagacac
     3901 cttgatatag acgcccttat caactagctt ctctatttgg ttctctgcaa gttccacttc
     3961 aaattcacga acggtatctc atttttcctt aaatgtctta aaggcttctt aaatgtctta
     4021 aaggcttcct caatctcttc agtggatact ttatccttat ctcgttcttc ttggaaagca
     4081 tggtactgtt cctgtaaatt ctctaatcct tctgaagcaa tgacttcctt atttttaaaa
     4141 taatcttgaa aaaatttgac atcatataat ttcttatcac ttattttttg atgacccaaa
     4201 cttatctttt gattattttc tttcaggata aaagttacat ttttttgttt taagtcaatg
     4261 gttagattca attcttttgc ttttgttatt aactcttcta aagaattgac acggttcaac
     4321 aaaaattcta aacgactttc aatctcttgc ttagcaaaat gcgttctaaa aaattcttca
     4381 tcatatagat ctcgtttgct gagttggcgt cctcgaatag gttttgtcat tgctcgatcc
     4441 gtcatcatga atcgactatg cttttgacta aaatcaatct gaacatgcaa ctgctttgct
     4501 ttctctaaaa aatcatcaaa ctatttagat tgctgcatca aaaaataaag acgttgtttc
     4561 aattcaaatt tatgactaga ttccctatat tttttataac ctcgataaga ataacgtttc
     4621 tcaataattt ttgcaccagc cattttagaa atacggtctg aaatcatacg gaggtttcgt
     4681 tccaaggcat agttccatat caacttttta tctgaattgc ggtcaatagc gttgattagg
     4741 atgtgattat gaacatgatc tttgtctgtg tgagttgcta cgataaactt aaaacgacct
     4801 cctgtcaatt ccatcatggt ctcataacca atgcggttaa tttcttcagg tgtcagatta
     4861 tcttcaggag aaaatgattg gatgagatga tgggcatgaa tagtttgttg gtgtttttct
     4921 tgtcggtcat ttctggactc gtacaacttg tcgttattag taaagttaac attgtacatt
     4981 tctaccattt ctgtatggct aggaaaatct aagtaattac tcatgccaaa atcagatact
     5041 aattttagat tatctgtttt atctggattg aggatatatt taatcaaacg cctacgatat
     5101 ttttttccgt gcgtcgcaaa gtgtttagta actaccatga aactccttca gtcttttcac
     5161 ctacacttga aattctttgt ccacttcctt aattaactct ccgactcctt tactcaattc
     5221 ttgtaattgg tcctgagaaa tcagatggct ttgattaatc gcacgcgcaa tttgattgat
     5281 gttatttcca atacgtctca attcaaaaac taactggtca taagtatcag tgttaatcgt
     5341 gacaaatgac atattgggat taagcaaaac ttttctggca tagactgaaa aattttgaca
     5401 attgctctta gcaatccgtt cattcaattg gtgtaattct ggatcagtta gaaatacttt
     5461 tttgagattg gtacgatagc gataaaccat tacatcctcc taacccatat atttttcacg
     5521 aaactcacga ctgagtggct gtacttgatt cacttctgca atcaattctt gaacgcaagt
     5581 caataagatt gagacgtgct cttgagtcac ttgatgattt tctcttgcga caaccagaac
     5641 ttcatacaca tctcgactaa tctgttcaaa cttttgagac tgccaaaggg agaaccaact
     5701 ttcaaaaatt ctatcctgat aattcttttt caataaattt tgacgaagaa attctgagaa
     5761 actatccact ttttgttccc tcatcatttc ttttatctgt ttttcttctt gtctcgtgat
     5821 tctaaattgc ttacggatta acttaatttc ttgtcccatc ttaccattct ttcaacatta
     5881 tacttgtctg acaatgtaag gctgcctcac attgtcagta cgattcctaa agacctcagt
     5941 ctctccttgt tcggtctttg cgagctcaga tattgtgtcc acaatatccc aaaaatcata
     6001 tcgccagcct atcaaaacct accagttttg acagctaacg ataagataac ttagtggctc
     6061 cgcccccaaa cccccaggta aaattcttag ttggattttt aaaagttgag aaaagtatag
     6121 cagaagaaat ttggggaagt tatcacaaat caagtaagga ctaatctcta acaccactgc
     6181 atgtaccgtc ctatatttat gatcagctcc catcgtttac attttcaggt cactgtcttt
     6241 tcataccagg aattagaatt gacaaattta attagagagg tattcattta attgttcact
     6301 tcttcgctcc atttccatta cagaaatttc ttcaatacta tgtgttcagc tgactcccat
     6361 gattcgttgt actacgtcct aaacgctcac ggaataagcc gtacatcttt cctagtttac
     6421 tctcgtaatt tacgtatata ggttacgaca cctatttgga ttttagagtt cctcatccgc
     6481 tatatatggc ttcaagtcac gattctgttt gtagaacctt gatttcgcta attccttcct
     6541 atccccccta cctcacgata ttcaggctta ggagtcgcta ttggtttcat ctgcagcaaa
     6601 tatccacaga ggacttggac ctcagatgta cactatgcct gtcggacaaa gaaaaaagta
     6661 ggctaaattt ctaacctact ttttctgact ctactctgat aaagccgtct catatataga
     6721 cattttatgc ttgaaggaat cttttcctcc ctctaattga ttgatgatat gattttttat
     6781 atatttttca ctaacatatc cctgtgattc aatccatccg ttaactccat ctgactccat
     6841 aataacaact tgatctgtca tagaatttgt tacaattgta gcattatgtg ttgctaaaat
     6901 aatttgacgt tgagctttca catctctaaa ctgctgaact aaatgcctgt aaatataacg
     6961 attgtctaga ttgtcttcag gctgatcaat aatcaatggt ctgaagtctt tagaataatc
     7021 actatatgct aataaaaaat caagcatggc tacaactttt tgtcccaaac ttaatacacc
     7081 aacttcttta tagagaatat caagttgttg cgaaaattct ttcgagttaa tattaaactc
     7141 caaaatcgtg tattttcttt agaaaaaatt tttcgtttat atgcacctga taagtgagaa
     7201 ccttttttca aaatgtcata actattgaaa tgagcaatat agttaactat tttttgatta
     7261 ttgaaatcct tcattatagt cagtgaatgt tgataacttc catctttctc acttataata
     7321 ttttcactta accattgatt aacccatgat tcttgttcat aatcatatat acatacaata
     7381 tgcaatttat ctgctgcact aatttctact ccatgtaaaa tatgaggatg tatatcataa
     7441 atcggataat ttttcattat gattgagact gccatttgta actttttaat acctttggta
     7501 gtattatgat cagttactac aactatttct attccatttt ttatgattgc ctctgctaac
     7561 ataagaaaac taatatattc ttttgaacta gaaaaaacaa ccttatcaaa attactaaaa
     7621 aaaattgtct tatcaacttt tattttctta ttttcaaaaa atatatcata tagttcatct
     7681 tcagtaattt ttctatattt cgttgcagtc caattagaaa atagcttgta atcataagaa
     7741 acaggtgtat gcaaatgaat taaagtcttg tgaaagctac cgaatttctc tttggaattt
     7801 gttagttctt gataaacaga ctttatcttg ttattaaacc ctctcatttc tattctccta
     7861 aaaatatcaa ttgtttattt ttcaagtaat tcattaagtc tcttttcccc cacctgcaac
     7921 atctccttct ctacctgact ccactcccca aagaataact cttgaagaac atctgctgct
     7981 gaagttgtat tttcttttga atcatataca caacttctat ctcgttggta aatttgaatt
     8041 ctttcaaaga tagctagttc ttccaatttt cgtgtgttat caactagatg atttacaatg
     8101 aaatcatgat gttcttttgg agttgcgcgt gcttgatttg gattgataat gtacagttct
     8161 tcataacgga taagagtact tagatacgac aattcaggct ttgtcgcaat taaggccaat
     8221 tgtacttcat atcccttatt tttcaagagt tgtgccgttt tctttggaac atcaattgtt
     8281 cgtaaagttc cctcgatcaa aagattgtat cccaaactac tcaattttgt tactaaagac
     8341 tctaccattt ttcctgcaaa atctttggta tattctacac tgtctttgcc atattcttgc
     8401 tgcagttcta aatagtgtgg atgctgagaa cgaaaactat caccatctat gataacaata
     8461 tttccttgaa attctttctg tttaatacga tgaattgtag tcttaccggc accactttga
     8521 cctccaagca aaatcgctat aggttgctta ctggactttt ttcctcttgt cagtgaacga
     8581 agattccttg ctaaagcatg tttgaattca ctatcagtat aatcttggat ttccattaag
     8641 ctaccatccg cttttcagat atctctaaca tacgttcaat tccatccaaa tagccactat
     8701 atctctctat ttcatcaaaa gtttctacta aatagatatt cgtatgaatc aaatcagaaa
     8761 gatcatcact cattaaaatc caaggattag attcatcatc aatgctaatt ccctgactat
     8821 cttgatagcg atagagtcga gataatagat tagcacctct ttctttcaca atttcaattt
     8881 ttaaagtcaa ttcataatct tcaacaggat tgagcatttt atcttctcct acgatatcga
     8941 cataagatac attaaacttc tgacaaatga tgtctattaa ttcggtagag actgaactcg
     9001 ttccattttc ataacgactc agactatttc gtgaaatacc tacaatccgt gcaaattcgt
     9061 gttgtgttaa gtcatgtgtt ttacgtaagg attttatgtt ctttccaatc atggcaaact
     9121 cctttatttt tataattcaa ttatagcata aaaaagcata atgcaccaaa aatggtgcat
     9181 tatttcttgt ttttctatcc taaactttac ttctataaaa gtcacctaaa gagccataaa
     9241 tatttattct tgtattaatt ttttcgatgc gcaaacgata tctacaaata catctataac
     9301 gattcacctt ttaggatttt tattgtcaaa attgccttta tctacaaaga caaaatgctt
     9361 ataaaatagt ttgaaatcaa ggattatcgc ctttttcgtt gtagcaacac tccacatgtg
     9421 attattaggc tattagcaaa gcgtatccaa tacctcaata aagtgttgtt tttactgata
     9481 ttttaactat attgtatagt ctactattta aatctcaatt aggatatttt atagaatacc
     9541 caaaattcaa caataactaa aagtaagatt ccattgtatt tccttgttgg tctttcatat
     9601 cacgaatttt atttttcaga atattaaagt tacaaatttc atctctaata tcgttatccg
     9661 ctctgtagcc attaatcatc tcagtcagag ttttcccttt actaccattc tttatagtag
     9721 tatttactct ttttatactg ctttcaaaag gctcatatgt tttatactta attacttcgt
     9781 ctttaaaatt aaataaaggt gtatccggct tagcattcca taaaataatc aaccacatta
     9841 tagcattata aagctgcttt gcagtttcat tccacttttc aggttataaa tttccactcc
     9901 ctgtattgtc atattcttgt ctattattta catgagaatc acctacaagc ggattaaaaa
     9961 gaggtagccc tttgtgatta attagttcta aaaaatccct tgccgcaccc atgcaacact
    10021 gaataacttc cttcttgcca tcaattttac tctcagcgat aaatacataa tattcatctt
    10081 caatagttgc tactgcatca ctatgttttt gttgatttgc taacagctta atatgtactt
    10141 taggctgaac ttcaaaatct ctaacaattc tttgtcttgt ttcatgtcct ctacaattca
    10201 tttaagttct cctttacaag tttagtaact agccttatta tactatataa ggtatgaaat
    10261 gtaccataat cttcatcaac tagtttttag aaaatcataa atccatctgg agagatgaca
    10321 agctgattat ccaattcttg gcaattgctt gctagtgcta ctgcttcttt ctctaaaaga
    10381 taatcttctg ttgattggtt gaatattgct ttgaggatcc gtccttcgta tttccattcc
    10441 agagctacta gatcagagtt gatttcttga aggctatact tgccatgcga aatgattact
    10501 gacgcgtatt tccgaatctt aatcagcctc ttcataaagt tcagcatatc attgtcactt
    10561 gatacacgtt cccaaggcat acaacgacga caatctggat ctggtcctcc agtcaaggct
    10621 agctcggttc cgtaataaat gcacggtgtt cctttttgta aaaagagaaa ggctaaggct
    10681 gatttaacca gttgaacatc ttcattggcc gtccacagga ttcgctctgt atcatgtgaa
    10741 tccaagagat taaacatgac ctctgaaatc tgctgcttgt aatacataga ctctccattg
    10801 atttcatcga tgaactggtc tgtcttctta attcctcgta agaaatagtc cttgatacta
    10861 tcagataaag gataattcat gacggcatgg aactcatctc catttagcca aggctgagat
    10921 gtatgccaga cttctcctag gatataaaga tcaggatttt tagctaaaac tgccttacga
    10981 aaatccttcc agaactgatg gtcaatctca ttagccacat ccaaacgcca agcatcgata
    11041 ttaaactctt caatccaata agtcgcaacc tttaaaagat aattcttgac ctctggattg
    11101 gctgtattta gcttaggcat atagtcctcg aaaccaaaaa catgataggg taagtctctc
    11161 ttattaacta gcttttcagt tgtcactggg aattgttgaa tatggaacca atccttataa
    11221 gcagactgtt caccattttt gacgacattt ttccattgaa gagattgcga accaatatga
    11281 ttaaataccg catccagcat gactttcatg ccacgatgat gcgcttgatc caccagttcc
    11341 cgaaatgtct ccttgtctcc aaaatgacgg tcaatttcaa agtaatctgt cgtattgtac
    11401 ttgtgattgc ttgcagattc aaagatggga caaagatata gtccagtaat acccaagtct
    11461 tgcaagtaat ccatatgatc aataatcccc tgtaaatcac caccaaagaa atcatcgctc
    11521 ttaggtgtga cagatgaatc ccagtctaaa gtcccttctg ggtttaatag agcattgcca
    11581 ttggcaaatc tttcaggaaa tatctgatac cataccgtat ttgaaaccca gtcaggaact
    11641 ttgcaggcat caatctcatg aagataaggc aacttaaatc cattcccaat tgcatgaaga
    11701 ttttctagag aattttccac acacccttta tcgccataca aaatattttg accttctgta
    11761 tctctgagct caaagagata ctggatacgt gcaaagtcaa ctgacacttc aacctgccaa
    11821 tggtcaaata aggtaccaga agttatcttg accatttctt ttgtatcctg ataaaactcc
    11881 tccataaaga taaaagggtc cccatagtgc aagttgatgc tttcaatgtc ccctttctta
    11941 gttcgaattc gaatatggag tttcttatcc ttataaagat aggcatactc cgactcaggc
    12001 ctatggtaaa tagcactcaa ttccatcaat ttgtctccta aagtattcat tcaaatttta
    12061 cgcaaacgtt ttcaaactca ataaatccag tatactattt ttgtaaacta tttgcaagga
    12121 atgtccgttg ttaaaggaac tatcttttaa agtatacaaa aagccttaaa aaaggctttc
    12181 tctatatact aatcctcttt cagttttgaa aatacttcta ctcgctgagc taggactttg
    12241 atttctacag gtaggttatc tgatttatct ccgtcaacat ctgactccaa ttcactatct
    12301 gaagagattt taatattacg cgctttgata tactcgatat tatcatttgc gacaacatcc
    12361 ccttttaata aatcaggaat gacggataat ttggagaata tagaggcatc tttcagaatc
    12421 aaaatgttgg catagccgtc cttgttttct tcaaagattt tcttatcagc gaagtaattt
    12481 gtcaaaagga ccaaaacatg gatagcttca ccaacataat ttccattttc tgtctcaacc
    12541 ttaatgttaa agacctgatc tgtcatgaca gacttcatgg tatttacagc ataggttaaa
    12601 ataccgaatt ttgttttgtc ctctatttca acattgtgaa tcgcctcagg cagagaaccg
    12661 atactaaaga tataaccaaa atagttgtca tttgctttac cgatatcaat cttattggtt
    12721 aaatcaaaat ccagttcgtc aattgcgcca tcgatgtctt gattgatttc caaaagtttt
    12781 gtaatgaggt tacccgtacc gcctgggata atccctaact taggaatgta gtctctctca
    12841 tcaatacctg aaatgacttc attgacagtt ccatctccac caaacacaac cactgcatca
    12901 tactgctcac gagaagcttc ttcagcaaaa tgtgttgcat ccagcgcttt ttcggtaatt
    12961 ttggtttcaa catattcaaa gtattctttt gctttattct ccagcttttc tttgtaatcc
    13021 aaagccttct cgccaccaga agtaggggtg ataattacca ttgctttttt cattgatttt
    13081 atccttaatt ttaaacagaa atgtttacat ttcgtcgtat gcaagtaaat gtaatcctat
    13141 tatacaatga aaatacagaa aagagaaatc tgacgtactg gagattaata cgcttttatt
    13201 ctattttccc atcgcctaac tacatccttt aagggttcat ccaagtaaga ataggcctta
    13261 tccttgatcc aatcaggaat accgtaagct gcctctgcta tgctacaagt gattgctgcg
    13321 agagtatcac tgtcgccacc aagtgagatg gcatttctta tcgcatcttc gaagtctcta
    13381 ctttcaagaa aggcgataat ggcttgaggg acagtttcct gacatgtttc gttaaaacga
    13441 tagttaggac ggatttcatc taaagtttga gatagattgt aatcgtattc tttttctata
    13501 taatccttaa ttcgtctttt acactctgta ggattgtcgt taattgattg ctcgtactct
    13561 ctacaataac caccaaagta aaaacgacat agaaagatag catcagctgt agccatagcg
    13621 cctttgacac cttctggatg attatgagtt acctctgcag aaagactcgt aagtcctcta
    13681 gatgatggcc atataccagt tttcgcataa aaaccacagt ccatgatcca agcacatgga
    13741 gaaatacgca tagctgatcc attcccaaag ctattataag gctcacggtt atcgctgttt
    13801 agccatgcat taaaccgagc accgtaatca gcattcggat acattctgcc atatttcttc
    13861 atcgcgtcaa tgaagtcatc tttttgtcca ccattcataa ttgcttctgc aacagcacag
    13921 gtcataaccg tgtcatctgt aaaaaagcag tccttccgaa ataaaggaaa gtcctttgtt
    13981 ttgatattgt tccattcgta aacagaaccg acaatatctc caataattgc tccaagcatc
    14041 agattcctcc ttgttcattt tgatgctttt tatattggtt atctaccata tttattttag
    14101 aaaataacat cctgttggat tttaaaaatt tcattttttt caaaataggg ttttaccatt
    14161 tctttccacc tagctctatg aaaattgatt gattttaaag gagataggcc ataatttccc
    14221 aatgcataac catcatttac ttcaacaaca agtgttctgc catcgcgagt aacaccgata
    14281 tctagtccat aagctattgg cgcatctttc caacatgata tcgcttcatc aattacactt
    14341 gcatcaaatt gtgcatgata atcacctgta tagggtcgaa catctaatac gcgaccatct
    14401 aacacaaaac aacgccattc agctatgaat tctacaacct cactaatcca tataggatag
    14461 tcgaaaggta gaccaatacc tattaaatca tgggttccat taacaactct tccagtaaag
    14521 acttttgaac cagctttagg cttaataaat tttccccaat tatcaggtat attcacaatc
    14581 tctcctaaaa taccagcata aatctttcga ccataaaact ctttaagctc aataggatag
    14641 tcatgaaccg gaacgtttaa gcccatcatt tttagtaatg ctctagtctc cattatataa
    14701 tctacaacta tatcttcact tgttaactct tttatttcag aaaaagattg atataaaata
    14761 acttcttctc cttgtaagta ggcacctact tgagcattgt atttattaat tgaaacctca
    14821 cttggtaatt tactttgtct aatataaaca accatttcat cactcctata tcactagtgt
    14881 tacaccaatt tgtaaaaaat aatagcaatt ttgctcttat ttttttgagt aaatagcccc
    14941 cataatatca tcgaaataat caacggtatt taggagtaat tcaataacct gggactttgt
    15001 tagtcgcatt ccccttctat ctctagcatc ttctactaaa ttttcaagtt tctctagatt
    15061 tttatcatcc aagctaatca ttattctatt tttatcggtt gccattttca tcacctcaag
    15121 ttaattctat cacaggtgta acactagtgt caactggctt ttataataca ttagtttaaa
    15181 agtggagagg atttttaaca cagtaacttt aaatctttgg tattaaaaaa ttttcacaat
    15241 atttatagaa ataaaatctg tctcaaatca gttatcaaat ctagtataaa ttatgagcgg
    15301 ctactctaat actttccctc taaacaagaa aaagacttac actcaagggt tttcttcccc
    15361 cccttcgtta taacgttttg actcttttac tagcaaaggt atatactcac aaggaacttt
    15421 ggttgactat tgaatctctc caacttcttc tttaacatat ccttctacat cttcaatctc
    15481 tacaaacatt gggtctaagt gacacaagaa atgccaaact tcgatccctt tttttctgta
    15541 aagaatcgct tcaccgtctt cacttccgaa aaagcttctg tcgatttcat atccgcggct
    15601 ttctaagaag tcttttgctt tacgatagtt cgtttctctt gtttcgacat aggctttaac
    15661 ttcatggttg ttaacgacat atgcatcaat ttttgaatat ccttcgatca ctctatcatt
    15721 tttgagggat aaatttgaaa tctctttcca aataatgttt acattttcct caggatcgaa
    15781 cataaattta gataaaggaa caatatttcc gttaaaaata atttccatat aatccggtat
    15841 gtttttagga ttaaaatact ccacttcaaa accatcttct gtttccagag tgtatcccgg
    15901 gatttgagct acaaaggctt tcccatcttc tatggaatca aatgctacta aatctttaga
    15961 ataatcattt tggtacaatt ccaatataac catcgataat ctctccattt tcattatcag
    16021 gctaatgtaa ataagcacgt cacctgacca attcaggctc tctgtatcat ctcatcatat
    16081 ttcctactta ctttacgagt cttataccca gaacacacct tatcgacctt cggtctcacc
    16141 tcgtcgcatt ggctgaacat ctacttttac tttgctgatg cttcaactcg tacaagcagt
    16201 gataccgcct cagcgtgatg cgtcagtggg actcaaaagg ttcggggaac cttttgagga
    16261 ttaactacgt ttctctaata aacttacaca ttcaacttgt tcatcattgt ccaaacctat
    16321 gttgagattt tcttctataa ttggtagctt aaaagtaatg gattttagcc attgtccgtt
    16381 agattgtttt tcttcataaa cttgaatttc agaaatcaaa gctgaaatta actgcctacg
    16441 ctctacatca ttcatgactt tatagagctt atcaaaatag atcagaacct tatatatgtt
    16501 atctcctgta agcttttcag cttcaatagt ctgtttcttt gctttcgcat caattagtga
    16561 tgattctaat tcatctagtt tgtcatacat acgatatagt ctatcatcta aatcctgttt
    16621 ccttctctta taatgcttat cttcaacatc taaattatct atttcctcaa ttagcttaaa
    16681 ctttgtagaa tgactctttc tcaattcctt ttggtaatta tctatttctc tttctatttc
    16741 agaggtatcc accttcatgt tgattttttc ttgcatcata gaagcaaatt tcggattact
    16801 tactatcttg acaatcacct ctgcaacagc atcatctaac aattcttctc taatttgctt
    16861 actgaatgta cacttatgac ctcttatcat ctgcctatgt ttacaaccat agtaataaaa
    16921 atctttatac tttgtgccat ctttcttttt cttgatacac ttgttcccaa acattcccac
    16981 tccacatatc gggcatttta caattccaga aagcaagtgt gtgcgtgtat cttttccttt
    17041 attcacatgc tcatatttct ttgcttgaga ttttagctta acctgagcag cttaccaaac
    17101 ttcatcggaa actatagctt catgtatccc ttcagatatt agatattcat cttgttcaac
    17161 ctgcttatat tcatttcttg taccatgaac tttttctaaa gttcttcttc caaatgctat
    17221 tttcccatta tatacaggat tctttaatat ctttcttata agacctgcat caaacaaagg
    17281 attcttacca ttctgtcttg ggatttttct aattccatga ttctctaagt atttagatat
    17341 cccattggct cctatcgtag tatttacata ctggtcgaaa atcgttctta ttgcaactgc
    17401 ctcttcctca tttataaaca gcttgccgtc ttcaagttta tatccatacg gagcaaagcc
    17461 accattccat tttccttccc ttgctttttg aatgcaacct tccattgttt gaatacggat
    17521 gttttctctt tctatttcag ccacagctga taaaacagaa atcattagtt tcccagcatc
    17581 tttagatgaa tcaatgccat cttcaacgca gataagatta actccataat cctgcattat
    17641 ctgaagtgta gaaagaacat cagcggcatt tcttgcaaat cttgataact taaacacaag
    17701 aacaaaagat actccatctt ttccagattt tatatcttcc atcattcgat tgaactgtat
    17761 tctaccttca atagacttgc cagacttccc ggcatcttca tactctccaa caatttcata
    17821 atcgttgtaa atagcaaaag ctttcattcg tgatttttgt gcccctaacg aatacccctc
    17881 tatctgtatt gacgtagata ctcgtgtata gaggtatact tttatttttt cttttgacat
    17941 agtattaacc tcaatataat ttttctatat catatataat ttttttaatt taagtttgga
    18001 ctatcatttc aagtatatta taacactttt attagtccgt ctcaatttgt gtttttgcca
    18061 tgtcaaaact atttttcatc tcttgatttt ttgctggcgt tggatcgggt agattatcta
    18121 aatctaaagc accagcatat tttgcaatca gatttgctat taaatcagcc aatccattcc
    18181 agtcattgtt caatatatac ctcctctaaa gttttatatc taataattat ttgtttaatt
    18241 aagttttttg acattgacaa gtgctttgga ttagcaacat aggaatctca cctccgcctc
    18301 tattccggat gagccggctt caaccttaga agtatcatta tcctcatttt cttcatagcg
    18361 gatagggtat ccctccctat attcaaactc ttacttatcg ctcactttct ttttgtttag
    18421 cagaactttt tttgccgaat tattcagccg aaagatcttg acggataggt tattacgctc
    18481 caaaaataat taacgtcttg tcttggtcta ttcaattgtt aaggttcaaa atttatcgaa
    18541 agttattaat ctttttaaaa tttgaccatc agaaaatatt tatcttgatg taacaaaatt
    18601 ctataaatta ccctcttata cttaacagtg aaaagaagtc tttcttggta accaattttg
    18661 aaatagaatt tgcttatata aaaaggtcca attcccactg cataagtagc agtgaaaatt
    18721 agaccctctt ggtaactgtc atctaaaagt cttctagcat ttctttaatt ttctaatcgc
    18781 ctttaaagtg tcatatttat atttttttat ctctggcata ctttgatacc tttttagact
    18841 taaagtcttt aatagtgcct ttccacctct ttttatctat aaagattctc ctacatcata
    18901 attcattttt ttatttaaac ctttctgtct tagtttgtct ttatcttctt cataccattt
    18961 taagattgtc acatagtggt tttgataggt cttaccactg ctttccatgt atctggatag
    19021 tttatttatc attatatctg tgtgtgagtt taatttttct tttagatttt tatattcttc
    19081 tttgcttaac cttacatttt tgaattctcc ataaaaaatg ggggtggact ttttatctat
    19141 ctctccctct ctctctttat ctatctctat atctttccat gtaattccaa tctggagtac
    19201 ctctactgtc tatcggtaat ttaattttga tatctggcaa tactgtgcta gatatttgat
    19261 ctttatattc agtatttttt aaagcttgcc taataattga agttaaatag aatgctactt
    19321 ctttattcaa ttctttattt tttaatttta aacaatgaat tttcatatct aggcttgctt
    19381 tatatttatg ataaaagact gctcctaaaa atgaaacaga tataaaattt tcaaaaactc
    19441 tataattttt atcatctata tcttcgtagt aacctaagat accattgtca atatttgtag
    19501 cactaattct aggagttttt ccatcgagta aatatctttt tggaatagat gagcctgttg
    19561 gtacttaact cgatttcccc tttttttcgg taataaatat ttctttttat tttgttgtct
    19621 gatatttttc ctacctgtcc tttgtaggat gagtattttc tagattttct tgaataactt
    19681 tttacttgaa gttttagctt ttgaactagt cgttgtactt tctttttgtt tattatcagt
    19741 cctgatcttt ttaatattgc tgttattctc tatatcctat ttttcattca tgatattctt
    19801 ttactaattt tatcttaaat tctgtgctgt atttgccatt aaaaaactga cctcctttag
    19861 ttagtttttt ggcctaactt ttgagggtca gttcaaaatt tgcgactttt aaatgaattc
    19921 caatattcaa ttattaagag ttaacatggt gcttgccaat aggaatcatt agaggcgaat
    19981 tggaaatagg gtcacgtata atttttgctt caagattaaa gatatcttta actagtttat
    20041 catttagtat atcttcaggc tttccctctg caacaagttt accttcttta attgcaaata
    20101 ggtaatcagc gtatcttgct gttagattta tatcgtgcaa aatcatgcaa atggttgtct
    20161 tatatttttg gtttagatca gtcaagaggt ctaatagttc tatttgatat gagatatcca
    20221 agtaagtagt tggctcatct aaaagtagga tacttgtatc ttgggctagg gctagagcta
    20281 tccatactct ttgcctttga cccccagaaa gttcttcaac taggttattt gctagatctt
    20341 caacattggc cttaaccatt gatctgttta ttatttcaag gtcatctttt ccaagactct
    20401 taaaaggctt tctgtagggg aaacgaccac ggcttacaag atcagctact gttattgatt
    20461 cagggattat tggagattga ggtaatatag ctatgtgttt tgctaaatct ttttctttat
    20521 aagaattaat tgatttatta tcaagcaata cttctccctc taatggcttt ataagtcgag
    20581 acaaggtttt aatgagtgtt gatttcccac aaccatttga cccaataata actgatattt
    20641 tttcttcagg tatttttata tttatatttt ccaagattat tttttcatca taaccgcagg
    20701 taagattatt tgaccacaga cctttcatta tatattcctc ctgttcattt ttattagtaa
    20761 gtatattaag tatggtgaac ctaacaagcc agttacaaca cctactggat atctagctgg
    20821 taaaatattt tgagagaata tgtctgataa caaaactagt aaaattccaa ccaatccagc
    20881 taatattggg cttcttttct tgccaatatt taaggctatg ggaccagcta aaaaagatat
    20941 acaagctatt ggtcctgtaa ttgaagtaga aaaagcagtt aaagatacag cgcaaaaaat
    21001 taaaacaagc cttgaaagct cgggatttgc tccaagtccg attgctattt cttcaccaag
    21061 ttcaataatt tctagtcttt tattaaaaaa taaaactaat atagtagcaa taatacttac
    21121 tattagaaca agaggtatgt catctaactt tgtaaaagat aaagagccac tgagccatct
    21181 cataacttct tgtaattcat atcttgctac tttcaacaat aaaaatgagg tgcctgctct
    21241 tgtgacagct tgaaaaccaa tacctaatat tatcagtctt gctgctgaaa aaccatcttt
    21301 tttagctagt aaaaataata ttaaagatga tgttagtcca caagttattg aaataattcc
    21361 agtagttaaa ctatttgttt ttaataccaa tatgcaaaag accgctgcaa tagatgaaga
    21421 acttgtgaca ccgattatat caggacttgc aagaggattt cttaacatag tttgaaagat
    21481 aaatcctgcc aatccaaaag accagccagc tataattcct gctaataatt ttggtaatct
    21541 aatttccata atcgaaaaac tagctccagg aacagtttca ctatttaaga ctttaatcaa
    21601 agttgaaaaa gaataacttt catctccgat aagtaaaatg aaaaatgata gactgattat
    21661 tattaataaa aatagtgagg aaaatagtgt tattctattt tttctttttt gaatacctat
    21721 aattaaattt tgcattagtt attaacccct ctatttttca tagttacata aataagtact
    21781 ggacccccga ttattgcagt aattatccct acttcaattt cacctggttt acctaacata
    21841 cggccgatta tatcacatat aagcaagagc tctgcaccta taaaagatga agaaatggtc
    21901 attgtgcgta tatctttgct tataaataag ccacaaaagt gaggaactat aagacctacg
    21961 aagccaatag gtccaccaat tgcagtaata cttgaacata aaagcacact tgcaattatt
    22021 gcaagtgatc ttatcctatt aacattaact ccaagaccaa cagccatttc atcacccata
    22081 gctaaagcgt ttaaatctga tgaaataaat atagctatca agtgacctaa aattataaaa
    22141 ggtagtagtg tagatataga agataatgta gctgctccaa ggctacctat ttgccaaaat
    22201 ctaaatttgt ctaagacgtt attattcggt aaaattaaaa aacttacaaa actgcttaaa
    22261 gccatactaa cacaagttcc tgataaggca agttttatag gggtaaggcc tgcttttccg
    22321 cttacagcaa tcgcgtatac aaaaattgca cttactaagc caccaatgat tgcaaaactt
    22381 atatggctta tgcttgatga aattcctaaa aaagaaagac caattactac actaagactt
    22441 gctcctgtgt ttataccgag tatacctgga tcagctattg ggtttctagt aactgattgc
    22501 atcaatacac cgcttatggc aagactagaa cctgctaaaa ttgcaaaaat agttctaggt
    22561 attcttttat atataattga tttaataaaa tcatcatttg tattgcccat tccaaaagct
    22621 gctaaaaaat ctctgatatt aatttctttt gttccaagct ttaatgacaa aaatattgta
    22681 ataagaagac ctaggcagat tacaaaaatt atgcctaggt ttatttgttt tttatttcgc
    22741 atttttgcat gcatttccta aaagatttag gtattcttca atagtatagt ttattgaaag
    22801 tggtgttgga gtgcatgagg ctgctaacgg tgtattatct ggaattacag caacggcacc
    22861 atttttaatt gcatttattt tacctaaaag aggatctttt tgtaaagctt caagagtttt
    22921 atcatcacca taagttatga ttacatcagc atcatttatc ttatttgctt cttctgcaga
    22981 aatttccttt gcaaaactat cttcactctc aaattctttt aatgattcag ggaaaactag
    23041 acctaaatct gttaaataat ttgctcttgg atctttgcta gtataaatcc agaattttga
    23101 tgtatctgca gcattaatca tagtaaataa tacttttttt cctttgattt ttcctttgat
    23161 ttctggatgt ttttctaatt ctttggatat acgagcttca gtatttttga ttaactcatc
    23221 accttctttt tccataccta aggcttttga atcaatttta atcatatctc tccataaagt
    23281 ttgccaaggt ttagatttgt atgctgctac aggagcaatt tttgatagag tgtcataatc
    23341 ttctttagtt ataccagaat aacctgctaa gataacatct ggtttagaat ttgatattgc
    23401 ttcaaagtta agtccatcca aatcgtcaaa taggttagct ttaccattta gttctttgat
    23461 tttttcttct gtccatggta aaactccttt atcagcactt acaccgtaat ttgcttttga
    23521 aaatccaaca ggaactattc ctaaagctaa tgctacatca tgatttcccc aagcaatagt
    23581 tgcaactctt tcaggttttt tatctaatat agtttgaccg aaagcatgat ctaaaactat
    23641 tttatcaggc gcatgctctt tagaagtatt ttcttcattt ttaacagaat tagaagaaca
    23701 agcgctaaaa actatacaca tagctaaaat agctattaga aaaaatttgt ttttcataaa
    23761 aactccttaa acatatttca agtctattgt atcgataata attatcattg tcaatagata
    23821 cagttacaaa tttaataata taattttttc aaaaaaggat ctagttttta tttttagtta
    23881 aattatgtct atatttttca ttaactcatc aataaaaatt tgtttgctaa tatctctttc
    23941 ttctgatttt gtttcaagga ttattgctac atcattcata tttttaggta aataccaacc
    24001 atctggctta tctgaatatc ccttgaagcc taattgatta aaagtagtta tttgaccagt
    24061 accttgattg atgttttctt caacttcatt aaagcctata cttctcttgc ataatcccta
    24121 acttgatctt ctgtccttaa agtcattttc tattcctcgt cactaaaatt atcttcaaaa
    24181 ctaataatat cattaggagt gcaagataaa gccttacata ttctttctaa attcttaaat
    24241 gtaattggtt tatcctttcc catttgtgcc ataacatttg ttgtaagtcc tgccatagct
    24301 attacatctg ttttctttag tccttttttt gctaactgta tccataatgg tttatagtta
    24361 agtgccataa tatccctctt tttttcttca ttagatttat ttaattatag cataaatatt
    24421 gatttcattc aatcttacat tgatttagtg atttgtttta gagaatttgt tgtgttattc
    24481 cgtccttatt ttgccataaa ttgttttaag tgggacctct tatccttacc atttttacct
    24541 cctaattttg agtataaaaa agacgataga gtttttaatt tctatcgtct gagatttaca
    24601 tattaaattt tcatcacaaa ataagactct ttaaagaatc tttaaattca ttggtatgct
    24661 gcttacaata tccattatta aaatggatat tgtaagcagc acaccaacta tttatttttt
    24721 gagttttaaa ctttggtaga agtcttgaac tccttatatt tacataacta tttgcctcta
    24781 taactgttat gacacgaaac caacaagcaa gttcaagcac ctccatatct ttattcaaat
    24841 gaggactaag atattcacaa actttatcat aaacctcttt atcttttttc ataaaagggg
    24901 catacaactt agcaaattta tcctaaaatt ttttgttgcc tttatcaatc atttcaacgc
    24961 tcctttaaat ataaaaatga tcctatcaat ctttatattg agcctatttt ctaattgata
    25021 ccttgcaaga aaggaataat agctgttcct ataccaagta ggaatgtaat aaatgcagaa
    25081 agtcatatac tgtcattctt ttctattaaa tcattttgaa gtgtctcaaa gcatccatga
    25141 tggcttcttc tttttcaggt gtacactgtg gaataatttg tttatccttt ttagacttgt
    25201 tgtaatgttc tcgtaattct attccacatt tcttttttat ctgtgcaata tataatgtcg
    25261 aaacttttaa ttcaaattta ttccaaacat attctttgat ttgagcatat gttgctttgc
    25321 tctccgcact tgtcaaatcc atctcatcca gctcaatttc aacacttatg tgcttatcga
    25381 catcgagttt ggacaaaagt gctaccgtct cgacgtgatg cgtttgagga aatagatcca
    25441 ccggctggac tttcttcaat tcatatccca actcttggta tagtttaata tcacgcgcca
    25501 tggttgcgac attgcaggag atataggcga tgcgatcggc tcctgtttgg gcgcttgctt
    25561 tgataaagct ttctgtcaag cccttgcgtg gaggatcaac caagataacg gttggttgaa
    25621 taccttcctt gagccatttc ttcatggcat tttcagccgt gtcacagaca tagtgggcat
    25681 tagtaatctt gttcaaagaa gcattcttct ggctattctc tactgcttct ggaatcagtt
    25741 caacaccgta gacttctttg acatgcttgg cgactgataa accaatggtt ccaataccag
    25801 aataggcatc aataatcaca tcatcttttt ttaactctgc aaagtcaatg gctgtttgat
    25861 agagtttctc cgccatttca gtattgactt ggtaaaaggc tgggccagcg atttggaagt
    25921 catttcccaa catctggtcc gtaatatagt cttgaccata aagagtgcgc cactccttac
    25981 caaaaatcgc attggtattc tggtcgttga tattttgcat gacagacaca atctctggga
    26041 actgcttgat aacttgttca atcaattggt caacacgaaa aacttttgga cgagttgtca
    26101 ccaaaacgac catgatttgt cctgaatagt gaccacgacg caccacaaga ttccgaatca
    26161 atccagattg ttccttttcg tcataaggtt ttaaatcaaa acgacggagc aggtctcgaa
    26221 gagctactac gacttggtca atgacaggat cctggataaa gaaatcttca aggggcatga
    26281 ggttatgcga attcttacgg aaaaatcctg tttccaagac accattcact cgacgaacgg
    26341 gcacctgcgc cttattgcga tacttgactg gatgttccat atcaagcgtt tcagcaactt
    26401 ctacatctgc aattccagca atcttgtaga gactgtcctt gacttgcttg gttttaaact
    26461 tgagctgttc tggataagaa aggtgtccta aatccgcgat tcctgaacgc aggtaagcca
    26521 aatctagatc ttgattacgg tgtggtgact ggacaaggta tttttcaact tttccaaagc
    26581 caatcttttt attgaccttg aggacacgca tgagaatttt ttcactcggt aaagcattct
    26641 ctacaaaaaa gaccaaacca tctaccttgg caactcctgc cccttcatgg gtcaaatcaa
    26701 caatttcaac ttctacaata tcatttttct ttaacattct tttctctttc tattggccga
    26761 caaaaaagac ggcaacgtta cggttaccat ccattatacc atgaaatttc ttaagaacca
    26821 aaactcttga agaagttgac aagggcttgc cagagggagc ttaagaagtt accaccgtcc
    26881 tctagcgcct tatccgcatc aaagttaagg ttgatatttt taaaactgtc gccagcttgt
    26941 gatacgatgc tttgtttaag gtcatttagg gttttagtga aatctgcatt gctgaggata
    27001 tcactctttg agagattcaa ggcaaaattg atgatgatat tgatctggtt tcctgttatg
    27061 acctgatcaa gtttgtaatt ttttaaggta tcttcaacaa tcttgcgtac atcttcttct
    27121 gtcagatttc ccttgctttc tttagctttg gcgagtcctg acttgatatc agctagggca
    27181 acgtttaatt tattagcatc atagcctgat ttgtccttgt tttcagcatt gatatctgac
    27241 aaagccttta gctcttcttg agccaaatct ttattggctt gcggcacctt ggctccatta
    27301 gcttctagcg aatagtagat cccagccaaa gcactctcac ctgtaactgg aataggggct
    27361 gctacagtga ttttggcatg ttccataccc agcgttactg ctgcgtttcg gtacatatcc
    27421 tgagtcacct tagtgatatt ttctggtgtt tcaatcttga cctcaagtgg cgatttgtca
    27481 cctagctttt gaatcttggc tgatgaatac aactgtaagc tagagtcatt ggccacattc
    27541 atgattttag aataaacatc aggtgtcatg gtcttgagtt ctttggtatc tgttgaggca
    27601 ttgtagccca gttttttaag agtttgattt ttttggtctt cagataggga ggaacctagg
    27661 acatattcag gttggacata ggtttcatcg ataacttttt gaacatctgt tgctgcatgg
    27721 acgctattca tagctgttac tgcccacaag accgcagcac tagtcagaaa gagtttcttt
    27781 ctcataggga atttcctcct ttacctttct agagtaatat atctatctta aagaaaactt
    27841 ataacaaaaa cacctgaact agccagatgt tgaaaagaga gtgaaacatt tgatgatgta
    27901 aaggttgagt tgcacctgtc tagaataata atagtttcct ccatttacat agagttcggc
    27961 accgtgaaaa atggaaatgg gatgaatata actataagtc tttccagtcg tattaccaag
    28021 caagggggca acagtctcac gagagtactg tttggctaga gccaaggtat tttccttgcc
    28081 attttgggcg ataaaatcga tataggcagg tccaaaatta taggcttgaa cagctgtcca
    28141 gatatctaca cccttcttct gcgccagata gagattgcct gtcagagttt gaatgccttg
    28201 ccgaatgcta gaggcattat cattgatggt gttggtggaa ccacttgcag actcactaga
    28261 ctgcataaca tcgccttctt ttccttttgt ttcagtataa atcatagcaa gcacaagctc
    28321 ttcgtttgct ggggtgtctt tttcactcaa tatttctcgc accatgggtt gataggtcat
    28381 gacttgtttg acatcttgat gaacgcggta agctttatag ccagcaaaaa ggaagactgc
    28441 tagtacaagc actcttcgaa ttcgtttaaa cattatttac tttggatatc ctcgatattt
    28501 ttgattaaga tagagtaggt tccattttcg ttttggataa actcaacaga ctcggcgtct
    28561 tgatagacgt tattgggaac gatgagctca attccatttg ataaggagag tttttggttt
    28621 tcaaatttct ttaattggcg actgacatca atttcatcaa attgaacagg ttctggtacg
    28681 gcttctttga cttggtcaat aaagctcaaa cgagccgtca gattgttgtc aaaaaggtca
    28741 ttagccaatt tctcaggtga caattcattg ctttcttcta ggttgttgaa aatagctgat
    28801 ttgaccttgg attgaaattg aaaatcatct gtgttaaaag attcagcaat tctctgggct
    28861 gttttttcca gttccttgat agatttttta ggagaaatct taggagcgac agcaagaaga
    28921 ttatctgaaa aatagttcaa aaaagtcccg ttgtacttga ttcgtttttc aatcaggtga
    28981 tacttgctac tctgaagatt gaccaccaag gcctcatcag ctcctgttcc aaatccaggc
    29041 aggttattct gagttagctt gattggatta tcaacttctc ctccgaggtg ggtcaaggtc
    29101 tcccgcaggg caattcgcaa gaaagcgaaa tgttctacac cttctttaga aaattgaaca
    29161 aaaatcaagt cattggtctt gagattttca gaaatgctaa actcctcttt ccagagatta
    29221 gccagcgtta ctgatgtctc caacaaatcg tctgtaatat gattgaagaa gggattttct
    29281 tcttcgaaaa tcccagtctt ggcttcatct gaatacacat gttcaatttt tttacgcagg
    29341 tattcttcga tttttggagt aatattgaga aacttatctg ctaagaacag ttcggtatca
    29401 tccggactga actggtgaat aatggctttc ttaatataaa tgtccataaa agttttagtc
    29461 ctcgtatagt aggaaggcat ctgtcaattc tttgactgca cttctcactt cttctaatac
    29521 agcctcattt tctgaattct taagggtttt aatgatgagt tcagccactt tgcgactttc
    29581 ttcttcacca aatccacgtg cagtaatggc tgctgctccg atacgaatcc cacttgtctt
    29641 gaatggtgac aagctttcgt aagggattga gtttttattt aaggtaatat tgacttcatc
    29701 caacaagttt tgagcaactt tgccgttttc tacaacttta gtcacatcaa caaggaagag
    29761 atggttttca gttccacctg aaataatacg gaaatcaggg tcttgcaaga agacatctgc
    29821 catagccttg ctgttcttaa ttacattggc agcatattcc ttgaaggctg gatccaaaac
    29881 ttctttgaag gaaactgcct tagccgccac aacatgctct aaaggaccgc cctgaatacc
    29941 tgggaaaata gctgaattga tttttttagc aagttcttcg tcattggtca aaatcaaacc
    30001 accacgaggt ccacgaaggg ttttgtgggt cgttgttgtt gtgatatgag cgtatggaac
    30061 tgggcttgga tgaaggccag ccgcaaccaa accagcgata tgggccatgt ccaccatgag
    30121 cttcgcaccg acagcatctg cgatttcacg gaattttgaa aaatcgataa tttgagaata
    30181 tgctgaagca ccagctacaa tcagttttgg ttttacttct tgggcttgtt tcaagatagc
    30241 atcaaagtct aagagttccg tttcaggatc aacactataa gaaacaaagt tgtaggtttg
    30301 accagagaag ctaacaggag ccccatgagt caaatgacca cctgatgcca aatccattcc
    30361 cataaccgta tcacctggct caatcaagga catgtaagcc gcacagttag tttggcttcc
    30421 tgaatgtggt tggacattgg caaatttagc accgaaaatt tcttttgcgc gttcaatagc
    30481 aagagtctct acaacgtcta ctacatcagt tccaccataa taacggcgtc ctgggtaacc
    30541 ctcggcatat ttatttgtca agatagaccc ttgagctgcc ataacagcct tggaaactac
    30601 gttttccgaa gcaattaact cgatattatt ttgttggcgt tcttcttctt tggcaatagc
    30661 attccagaga tcagcatcat atgctttaaa atcatctttg tcaaaaatca taggtcttct
    30721 cctttattgt gtgactagtc cattagtttg attttacaat aagaaaatca aactaaaaga
    30781 tgcgaataaa ccgtttctgc attttatcac aagtatagcc aactttttca taaaatgcat
    30841 gagcacccag acgatgattg gcagaattta agcggataaa cccataacca catctttttg
    30901 cttcttcttc caacccttgt agtaaacttt taccaatacc ttgaccttgc gcttgaggtg
    30961 aaactgctaa agctaagata ttaaatcctg ctttggaata gagtgattcg taaacttcag
    31021 cgtggacata tccaagtaag acatgattag ctgcatcctc atagccaagt aggaaatgat
    31081 gggaatcctg agacagtcta gctagttggc tagccgtttc ctctggacta aaagtataac
    31141 ccaaagcctc ttggttgatg tcacatatcg ctttcacatc tgtttcttgc aaatctctta
    31201 gcatttcaag cctcctcaaa agaaatctct ggcaaccgag caagaatatc ttctcgttta
    31261 attgcgcctt ggcgtaagat tttcaccttg tctccagaca aatccacaat agttgaatcc
    31321 tgtccagtta gaaaagcatc gtcttccaga cccagaacct cttggtcaaa atccttcaga
    31381 atttgttcaa aggttacacc acttgcctga cctgagatat tggcagacgg cccaatcaag
    31441 ggacctgtct ctcgaattaa atccagtgtg atagggtgac tgggcatccg aaatccaata
    31501 gttgcaaggt cagaatttac ccaataggga actcggtcat tggcttcgag aataatggtc
    31561 aagggacctg gcaaaaaggt ctctacaagt ttttgtagat aagttggctg attcttagaa
    31621 aagtgcaaga tatcctcgaa agaggcgata ttgagattga gtgccttatc tctaggacga
    31681 cgtttgagtt ggtaaacatg gtcaactgct ttttcatcta aggccttggc aaaaagacca
    31741 taaacagtct ctgtaggtag aacgacagct ccaccatttt ccaactcttg tctaatcctg
    31801 tccatcatca accacaacca tcctattttg accaaattgg tctttgagtg ttcgaacttt
    31861 tttttcagga agatgttttc taaaaagttc gggaacactt tgaccttgct tgtatccaat
    31921 ttcaaggtaa atcttaccac catccttgag atagtctttt gcatcttccg caattctaca
    31981 gtaaatagct aggccatcct cgtctgcaaa gagagctaga tgaggctccg aatgcaaaac
    32041 attcaaacct acctctgact catcttcacg agagatatag ggtggattgg atacaattat
    32101 atcatatttt tcagaaattt ctgcaaaaca atcagatttt ttaaaaaata tattaagatt
    32161 ttgatcgcta gcattctctg atgcaagctc taaggcctct tgagaaacat ctgctgctgt
    32221 cactgaccaa tctggtctat tttttgctag agcgagagca atagctccac tacctgttcc
    32281 aatatccaga actgagagat tcgtctcaag attttcagtt agaataagct ccactaactc
    32341 ctctgtttct ggacgaggaa tcaaaacccg ttcatccacc tttaaatgca ttccataaaa
    32401 atctgcctgt ccaatgatgt actgagctgg cttgtgagct gctagttgct ggtaaatatc
    32461 ttctacaaat tgtttttctt cctctgttgt cacctcctgc tggagggcaa aaataaagtc
    32521 tgtaaaagat agatttttca gactacgata gacaaaagag aggctttccg cttcctctcc
    32581 ttgtcttatc aactcttctt caaaatttga aaataattga gctaatttca ttatttgttt
    32641 aattcttcta gtttttgtgt ttggtcataa agcaccaagg catccacaac ttcgtccaat
    32701 ttaccagaca aaatcgtatc tagtttttgg agggtcaagc cgatacggtg gtctgtgaca
    32761 cggttttgtg ggaagttata agttcggatc cgttctgaac ggtcaccagt accgattgtc
    32821 gacttacgct cagcgtcttg ttcatcctga gcaatctgag caaagtggtc agcgacgcgt
    32881 gcacggataa tcttcatagc cttctcgcgg ttcttctgct gggtacgttc ttcctgcatc
    32941 tcaaccttga tattggttgg caagtgaacg atacgaacgg cagtcgcaac cttattgacg
    33001 ttctgtccac cagcaccaga ggcgtgatag atgtcgacac gaaggtcttt tggatcaatg
    33061 tcgtattcaa cctcttcaac ttctggcata acaagaactg tcgctgtcga agtatgaaca
    33121 cggccttggc tttctgtcac aggaacacgt tgcacacggt gggcacctga ttcatactta
    33181 agcttagagt atacagactg acctgaaacc atagcaacca cttctttaaa accaccgaca
    33241 ccattcatag aggcttccat gacttcaaag cgccaacctt gggcttccgc atacttttgg
    33301 tacatagtta gcaaatctcc agcgaaaagt gccgcttcgt ctccaccagc tgctccacgg
    33361 atttcaagga tgatattctt gtcatcgttt ggatcctttg gaaggagcaa aattttcagt
    33421 ttttcttcat attcttcttt ttcagccttg gcatctttga gttcttgctt ggccatttct
    33481 tccaagtccg catctccgcc tgattcctta atcatctctt cggcatcgac gatattttga
    33541 aggacttgtt tatactcacg gtaggctatt acggtgtcac gattggaagc ttcttctttt
    33601 gaaagctcca taaaacgctt ggtgtctgaa acgacatcag ggtcactcag caattctcct
    33661 agttcttcat aacggtcttc tacagcttgt agttgatcat agatgttcat ttttcttcat
    33721 tctccttatt gatttcaggg gcaaaataat gtttacggca aaccgagata taggtttcat
    33781 taccaccaat ctggatctgt tctccatcgt aaacgggcag tccatcctgt gttcgcaaca
    33841 ccatggtcgc ctttttcttg caatactgac agatggtctt gatttcgtca atcttgtctg
    33901 ctaaaagcaa gagatatttg gaaccttcga acaattcatt gcgaaagtca tttttcaagc
    33961 caaaagccat gacgggtatg tctaactcgt ccacaacacg agctaggtca taaacatggt
    34021 ggcgtttgag aaactgggct tcatcgacca aaacacagta aggtttttct ggtaggtctc
    34081 ggatatagcc aaagatatcc gttgtttcct caatcgcaag ggcagggcgt ttcatgccaa
    34141 ttcgactcga cacatagcca acaccgtcac gcgtatccag agccgaggtc ataatcacaa
    34201 caccttttcc ttgctcctcg tagttatagg ccactttgag aatctcaatc gttttaccag
    34261 agttcatggt cccataacga tagtacaact gtgccatgtt tcttgcttca cgtccatttc
    34321 taaatttttg ctacattcta gtatatcata attttcttaa gctttaaacg gcaaaatgtg
    34381 gtaaaatgga agaaatcaaa actagtggag gaagctatta tgccatttgt acgcatcgat
    34441 ttatttgaag gacgcacgct cgagcaaaag aaagctcttg ctaaggaagt aacggaagcg
    34501 gttgttcgca acactggagc acctcaatct gctgttcatg tcatcatcaa cgacatgcca
    34561 gaaggaactt atttcccaca aggggaaatg cgcaccaaat aagctagctt aagcagaatt
    34621 gcttaggctt tttcaatctc caagtagcac ccattgaaga aatagtctaa atttgttaca
    34681 atttgaaaag agacttggaa aattccaaga aaagagcaat taattaaagg aaacattatg
    34741 attacacgtg aatttgatac catcgctgct atctctactc cactaggtga aggggctatt
    34801 ggtattgtcc gcctgagcgg aacagacagt tttgctattg tgcaaaagat ttttaaagga
    34861 aaagacttga acaaggttgc cagccacact ctcaactacg gtcacattat tgatcctctg
    34921 actggtaaag tcatggacga ggttatggtt ggggctatga agtctccaaa gaccttcact
    34981 cgtgaggata ttatcgagat taacacccac ggtgggattg cggtgaccaa tgaaattctc
    35041 cagctagcta ttcgtgaagg ggctcggttg gcagaacctg gtgaatttac caaacgtgct
    35101 tttttaaacg gtcgcgtaga cttgacacag gcagaggctg tgatggatat catccgtgcc
    35161 aagactgaca aggccatgaa cattgcggtc aaacaattag acggctccct ttctgacctc
    35221 attaacaata cccgtcaaga aatcctcaat acacttgccc aagttgaggt caatatcgac
    35281 tatcctgaat atgatgatgt tgaggaagct actactgccg ttgtccgtga gaagactatg
    35341 gagtttgagc aattgctaac caatcttctt agaacagccc gtcgcggcaa aatccttcgt
    35401 gaaggaattt caacagctat catcggacgt cccaacgttg ggaaatccag ccttctcaac
    35461 aacctcttgc gtgaggacaa ggctatcgta acagatatcg ctgggacaac acgagatgtc
    35521 atcgaagagt acgtcaacat caatggtgta cctctcaaat tgattgatac agccggtatt
    35581 cgtgaaacag atgacatagt ggaacaaatc ggtgttgagc gttcgaaaaa agccctcaag
    35641 gaagccgact tggttctact agtgctaaat gccagtgaac aactgactgc gcaagacaga
    35701 caacttcttg aaattagcca agataccaat cgcattattc tacttaataa aaccgacctg
    35761 ccagaaacga ttgaaacttc gaaactacct gaagacgtta tccgtatttc agtccttaaa
    35821 aaccaaaaca tcgacaagat tgaagagcga atcaacaacc tcttctttga aaatgctggc
    35881 ttggtcgagc aagatgctac ttacttgtca aacgcccgtc acatttccct gattgaaaaa
    35941 gcagttgaaa gcctacaagc cgttaatcaa ggtcttgagc tggggatgcc agttgatttg
    36001 cttcaagttg acttgactcg tacttgggaa atcctcggag aaatcactgg ggatgctgct
    36061 ccagatgaac tcatcaccca actctttagc caattctgtt taggaaaata agaaaaatcc
    36121 atgatccttc attcggtcat ggattttatt gtctttatta gtaatctggt cttaagaccc
    36181 ctgttacagt tgccttagtt gcttcgtagt cgccatctac gacaaccttg ataatgcgtt
    36241 tggcatcttc ttctggtgct ggaacaagag gtagacgagt gggtccagct tcaaatccca
    36301 tatagttaag aattgcctta actggagcag gacttggata agagaagaga gcattaacct
    36361 taggaatgaa tttacgctga attgctgcgg ctttcttcat atcgctttct gcaatggcag
    36421 taaacatctc gtgcatttca tccccatttg tatgagaggc aacagaaata accccatccg
    36481 ccccaaggtt catggcatgg aaagcatctc catcctcacc tgtataaatc aagaactctt
    36541 caggcttgtg ctcaatcaag taagccatat tagccaagct agtacattct ttgacaccga
    36601 taatatttgg atggtcagcc aagcgaagca tggtttctgg agtcaattcg acaactacac
    36661 gccctggaat gttatagata ataattggta ggtcagaagc atctgcaata gccttaaagt
    36721 gctgatacat cccttcttga gaaggtttgt tgtagtaagg aacaatagca agcccagctg
    36781 cgaaaccacc aaattccgct acttctttga caaactcaat agagtcacgc gtatcattag
    36841 tacctacacc cgcaatcaaa ggaacgcgtc cattgacaac cttttgtaca gccgcaaaca
    36901 actccaactc ctcatcgtgg gtcaaagttg gactctcagc agtcgttcct gcgagaagaa
    36961 ttccatccgt atgatgggcc aataaatgct caatcaaggc tggaatagca tcaaagttaa
    37021 tggaaccatc ctcatggaag ggggtaataa aggctgtaat gattttacat tcttttaaat
    37081 cttgataaga cacgaaaaca cctctctatt tcaaagaagg agttcatctg aactcctaaa
    37141 cgatatgact attttaattc aaatttcaat tcagctgttg gacgaaccaa tccacgttca
    37201 tgaagagttt cagcaatctg aactgagttc caagcagcac ctttgagaag gttatctgaa
    37261 acaacccaca tgtgaattcc tttttctgca tccaagtctt tacggatacg accaacaaag
    37321 gtatcacgtg aaccaactgc attgatggct tgaggataga tttgatgaac tacatcatct
    37381 tcaagaacag cacctgggaa ggatgcgata gctgctttga cttcttcaat tggagccact
    37441 tcctttgttt caatataaac tgactcagag tgagctgaca agactggaat acgcacacat
    37501 gtcgcagata ctgcaatgct atcatcttcc ataattttct tagtttcctt ggtcatcttc
    37561 atctcttcgt acgtgtaatc attatcagtg aagacatcaa tttgtggaag agcgttaaag
    37621 gcgataggat aatgtttctt gtcaccacct gaaggcaaga tttccgcatg caaatcacgt
    37681 ggtttcactc catcattcaa gacctcacga agttcacgtt gtgtctcaag aattgctccc
    37741 ataccagcac ctgaaacggc ttgataagtt gaaacaatga tacggtccaa gccccatttt
    37801 tggcgaaccg gctcaagagc caccatcatt tggattgttg aacagttagg gcaggcaatg
    37861 attccgttgt gggcatcaag tgcatgagca ttgacctctg gaacaaccaa aggaacatct
    37921 ggattttgac ggaaataaga tgtattatcc actactacca cgccagcttt tactgcgtat
    37981 ggtgcatact tagctgatgt cgaaccacct gctgaaaaga gagcaatatc aactccttca
    38041 aaagctgttt cagtcgtttc ttcaatcgta atatcttgat ctttaaattt caatgactta
    38101 cctgctgaac gtgcagaagc aaggtaacgg attttatcga ttggaagtgt tgattcttcc
    38161 aacattttta tcatctgagc accgacagca cctgtcgcgc cgactacagc aactgtatat
    38221 cccataaata acctctttcg gaattttcta aaaatttcta taatagaagt attatactac
    38281 tttttccatg aattgcaaag ctttttcatc attttttgga aaataaaaat ccccaatcgc
    38341 tagactaggg attaagaggc atcttcatgt gatgagcagg ttcacacaac tcatcaaggt
    38401 ccgctcctgc gttatgacct ccttatgctc aatagtagcc cgaagactac ctatcgactc
    38461 atcgcatcta ctattctact agatagagtc atttttgtca acagccaaaa ctctttatac
    38521 tcttcgaaaa tctcttcaaa cttcgtcagc gtcgccttgc cgtatgtatg gttactgact
    38581 tcgtcagttt catctacaac ctcaaagcag tgctttgagc aacctgcggc tagtttccta
    38641 gtttactctt tgattttcat tgagtattag ttttatttac acaggtgcat aagaaatata
    38701 ttggaaaaat tgggggcaga cataacaaaa acccttgtaa atcaagggct tttctgtcta
    38761 tttaatgcta attgccggga ttgaaccggc gacctcatcc ttaccatgga tgcgctctgc
    38821 caactgagct aaatcagctt acttaagaag tatactatat tcagaaaaac ttgtcaagtt
    38881 tccttagaaa ttttccatag gaaaaagcca ggtaagaact acctagctta tcttcttcta
    38941 tttacttaaa tcaattcgac gaccaatttt accaataata atcgctccaa taatcaatga
    39001 agcaaattca ccgattcctg ttgtaaacca agtaaggaag aatggtaatt ctgctacaat
    39061 atttaactct gcagcgatag taaacatgga aattgaaaag aggattgaaa agaagaaatg
    39121 atcttttcga atcaatccgt taaagaggta atccttgcta tattttgcaa aaagccaaac
    39181 acctaaactg aggaatacta aggttgaacc accaccaaca aagacatcta gcagtccgaa
    39241 gctaaagaaa ttagcaatca tacaaccgat ggtaactccg atgatgtact taggattgta
    39301 aaaagccata aagttcatca tttctgaaat acgaaactga taagcaccat agctgatggc
    39361 atttaggggc ggtgtgacag tcaaaacgac gtagatagca gcgacgattg caatatctgc
    39421 aacatcacga atagttaatt ttttcatctt ttctcctttg gcggttatcc gcgtgtaata
    39481 tgcttggtga aagaagctaa gcaccaaggg ttgattcatt caaccttact agtatagcac
    39541 aatagcctgc tttatgctat acttaaagca tgaaaaaacc tattcaaaaa ttttttaaca
    39601 atgaaatctt agcctatctc ttttttggcc ttgcgacaac tctagtttcg attctctcac
    39661 gactagtcat ttatcaacta agtcatcagg aactccttgc tacggccttg gcaaatatta
    39721 tcggtatctt atttgccttt atcacaaatg atacgattgt atttaagcaa aaaaagaaaa
    39781 atagacttat ccgtttagtg aaattttctc ttgctcgcct ttctactttt ctgttagact
    39841 tgtttttaac ttttctcttt gtaacccaat ttcctcacat catagggcaa ttcgtaactg
    39901 aaaacattga taagataaat gctattgaaa caataattgc acaaatattc attattatca
    39961 tcaattatat cttgagcaag gtctatattt ttagaaaata atgctccatt tatgagcctt
    40021 ttaattgcct tctataatga tttaggatac aatgaaacac gaaacattga aaggaaaaca
    40081 gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac cttgctgtcg
    40141 gtgttgtcat tgcctctgct tttggtgcta tcgttacttc acttgtaaac gacattatca
    40201 ctcctcttat tttaaatccc gctttgaaag ctgctaaagt cgaacgtatc gctcaacttt
    40261 cttggcatgg agtcggctat ggtaactttt taagtgctat tatcaatttt atctttgtgg
    40321 gtaccgccct cttctttatt atcaagggca ttgaaaaagc acagaaactg actggcataa
    40381 agaaagaaaa aactgccgaa aaaaaaccaa ccgaattgga agtccttcaa gaaataaaag
    40441 ctctccttga gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa tccttttttc
    40501 tttactcttt gggtaacttg cctgcttttt caagcatttt tttgataaga taaggcatct
    40561 taacattctc ccgccctttt ttctcaatca gttttttcac aaattctggc atttgtaaat
    40621 cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca tcaaaggtct
    40681 cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact gtattgacca
    40741 aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag tttaccccaa
    40801 attcctggca taagtcataa cattctacat cattgtcaaa caagacctca atatgctcac
    40861 tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt tctctgagat
    40921 actccaaaac atctggttta atccatggaa taccaatatc actctcactc tgccaagtgt
    40981 tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta ttttcgaaaa
    41041 tttggtctat ataaggatca ccaaagtcca aggcaaaaat gggcacacta tgggctgaaa
    41101 agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca atttcatctg
    41161 cccaatagtt taagagcgct tcttcctgat accagtcctt aatgaccaaa aattgaattt
    41221 gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa tggggctcca
    41281 aaatcaagca aatacactgt tcaattccgt ctgcttccat ttgaccgatc acatctggaa
    41341 taaagggact ggaaaactta ttagcaaagt agacactgta ttcattcccc agcctagctt
    41401 ctaccaaggc aacctcttga cgggtaattt tttgcagagg cgtgcctcca attcgtacat
    41461 aattatcata gagagtctga atctcgtggt cttggggtct caccccacga cgaatatttg
    41521 tgaaaaaatc agctacacct tcaaaggtaa tctcttctgg tgaaccgaag gtcatcatta
    41581 aaattgcttt tttcatgact gcttccttgt gatgttttta tcaagtttat tttatcatta
    41641 attaatcgat aagtaaacgc taatataggg acattctcaa tcttctgtct tttgtttttc
    41701 tcttctttct atgatacaat ggaaaaaata aattcaaaag gagttttttt atgacttatc
    41761 caaatctctt ggaccgcttc ttaacctacg ttaaggtcaa cacgcgctct gatgaacact
    41821 ctactactac tccaagtaca cagagtcagg ttgacttcgc aacaaatgtc ctaattcctg
    41881 aaatgaaacg tgttggactg caaaatgttt actatctacc gaatggtttt gctattggaa
    41941 ccttgccagc caacgatccg tctttaacac gtaagattgg ttttatatcg cacatggata
    42001 ctgctgattt taatgctgaa ggagtcaatc cacaggtaat tgaaaactac gatggtggtg
    42061 tgattgaact agggaattct ggtttcaaac tcgatccagc tgacttcaag agtcttgaaa
    42121 aatatccagg acaaacgctc atcacaacag atggaacaag cttgctaggt gctgatgaca
    42181 agtcaggaat tgctgaaatt atgacagcca ttgaatatct aactgctcat cctgaaatta
    42241 agcactgtga gattcgtgtt ggttttggtc cagatgaaga aatcggtgtt ggtgccaata
    42301 aatttgatgc agaagatttt gatgtggatt ttgcctacac tgttgatggt ggtccactag
    42361 gtgaacttca gtacgagact ttctcagccg ctggtgctga attgcatttc caaggccgta
    42421 atgtccaccc tggtactgcc aaagagcaga tggtcaatgc ccttcagcta gcaattgatt
    42481 ttcataatca acttccagaa aatgaccgac ctgagttaac tgaaggttac caaggttttt
    42541 accatctaat ggatgtgaca ggtagtgttg aggaggtgcg tgcaagctac atcattcgtg
    42601 attttgaaaa agatgccttt gaagcgcgta aagcatccat gcaatctatc gctgataaga
    42661 tgaatgcaga acttgggagc taccgtgtca ctctcaactt gacagaccag tactacaata
    42721 tgaaagaagt cattgaaaaa gatatgactc caattaccat tgctaaagcc gttatggaag
    42781 atctaggtat cacgcctatt atcgaaccaa tccggggtgg aacagacggc tctaagattt
    42841 cctttatggg aatcccaact ccgaatatct ttgcaggtgg cgaaaatatg cacggacgtt
    42901 ttgaatacgt tagccttcag actatggaac gtgcagttga taccatcatt ggcatcgtag
    42961 cttataaagg ctaaaaagac gaggtagctc agctacttcg cctttctttt tattctactg
    43021 gtttttcttg atttccagta gttgtagaag attctgttgt ttcattttct gaagttgatt
    43081 cagcaggttt agaatctctt gtattgcttg gtttgttttc gtcgctagca atttcaatgt
    43141 tagattctgc agttgcgttt ggttggttct cagcactggt gttatcacta tttgcttcag
    43201 catttcttgc tggacttgtt tcttcacttg cgctagcttt tgactggatt tgatgattca
    43261 aaactagaat agcttttgtc gattcaagta aagctgtttt gtctttactc ttagcagaaa
    43321 gttgatctaa taatgcatcc accttatcaa agtccgcatc agatccatta ttactttcta
    43381 aataagagtg aagcgacatg agaatatcgt agagtttttg atagagtaca agtgtctgag
    43441 aatcttgctc agcattttcc ttttcttgtt gaagggcgct agcgatacga gtcaagacat
    43501 cttttacctg actgtttact tcatccaagt ctgcatcagc cttgtttgtg gcagctttta
    43561 gattttctac ttcttctgcc aaggattgtc tgattccttc ttcatggatt tgttccaaga
    43621 gttgatttac cttgctcaaa agactttcta cttcttcctt gctatctgtc gcagattatt
    43681 ggttgccatc taccatgtac tcctaaaaca ggagagttat aatccaagat tacaaggcct
    43741 tacagaaata agaaatccag ataagacaat gttcgtccaa gacgctattc gcttcgcaca
    43801 gcagcacgga ttcaatatgc tttaatttta aagtttaggt gtcaagacct ctttttagtg
    43861 tgcccaaaat ttagagaagt aatcaatcaa ctaactttta tttttttcaa actttcagta
    43921 aactgaccta aagctaactc aatctgtctt tgtagatgct tctgctatca gctagaagtt
    43981 gatctacttt tgccaagact gccttctcat caaaagttcc aggttgatag ttggattgca
    44041 gggatggaat cttgtttttc aaagccgctt catatccctt agtttgaacc ttgatgtagt
    44101 gattgtggtc gccatgagga atcacaaaac cttctgaatc ttcacttata attcgattgg
    44161 catcaaaacc atgaccatct tcttcctcat gatggacatg tagtgacgga ttacttaata
    44221 cagaactaga agaacttcct acctcttccg tgttagagtg tgatggggga ttgttaagag
    44281 atgacttagg aatatagtga tagtgatccc catgtcttac tatataagca tcacctgtat
    44341 ctctgacaat atcattaggg ttaaagacat atgtggctgc taattcacct gccgacaagt
    44401 cactctcagg aatgaaatga tagtgaccac catgtggtac tatagtagat tgaaatagaa
    44461 tatgagcaaa ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga
    44521 tgtgctattc taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt
    44581 aattttgcta cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt
    44641 tggcgattga tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa
    44701 acataatact ttccatctac cttaataata tatcctccct taacctaact gacgatatct
    44761 tgatctttcg gctgatagtt gggggctttc attaatagct cttcactaaa gagcgcatca
    44821 aaaggaactt taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga
    44881 tctgtaatct taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct
    44941 ggagtcaaat tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt
    45001 ttatctgtat tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa
    45061 aggataacaa cagatccagc tgctatatat ttctttttaa atttcataat tacctcattt
    45121 ctataattat ttatatgatg tcttcattat taaatgatta aataaattaa ttaaccaatt
    45181 aattaactag taaatattcc acctcttttt
//
LOCUS       DBERYS010000013        42788 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.013, whole genome shotgun sequence.
ACCESSION   DBERYS010000013 DBERYS010000000
VERSION     DBERYS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.013"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(19..1977)
                     /gene="ftsH"
                     /locus_tag="ACL8LA_000803"
     CDS             complement(19..1977)
                     /gene="ftsH"
                     /locus_tag="ACL8LA_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGS1812592.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAHHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(1993..2535)
                     /gene="hpt"
                     /locus_tag="ACL8LA_000804"
     CDS             complement(1993..2535)
                     /gene="hpt"
                     /locus_tag="ACL8LA_000804"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGS1812593.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2539..3816)
                     /gene="tilS"
                     /locus_tag="ACL8LA_000805"
     CDS             complement(2539..3816)
                     /gene="tilS"
                     /locus_tag="ACL8LA_000805"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGS1812594.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3813..5081)
                     /locus_tag="ACL8LA_000806"
     CDS             complement(3813..5081)
                     /locus_tag="ACL8LA_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGS1812595.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5074..5196)
                     /locus_tag="ACL8LA_000807"
     CDS             complement(5074..5196)
                     /locus_tag="ACL8LA_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGS1812596.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            complement(5201..5569)
                     /locus_tag="ACL8LA_000808"
     CDS             complement(5201..5569)
                     /locus_tag="ACL8LA_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGS1812597.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5562..5828)
                     /locus_tag="ACL8LA_000809"
     CDS             complement(5562..5828)
                     /locus_tag="ACL8LA_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGS1812598.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5886..9395)
                     /gene="mfd"
                     /locus_tag="ACL8LA_000810"
     CDS             complement(5886..9395)
                     /gene="mfd"
                     /locus_tag="ACL8LA_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGS1812599.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9396..9965)
                     /gene="pth"
                     /locus_tag="ACL8LA_000811"
     CDS             complement(9396..9965)
                     /gene="pth"
                     /locus_tag="ACL8LA_000811"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGS1812600.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(10037..11152)
                     /gene="ychF"
                     /locus_tag="ACL8LA_000812"
     CDS             complement(10037..11152)
                     /gene="ychF"
                     /locus_tag="ACL8LA_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGS1812601.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11236..11430)
                     /locus_tag="ACL8LA_000813"
     CDS             complement(11236..11430)
                     /locus_tag="ACL8LA_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGS1812602.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEVTRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11495..12631)
                     /gene="dnaN"
                     /locus_tag="ACL8LA_000814"
     CDS             complement(11495..12631)
                     /gene="dnaN"
                     /locus_tag="ACL8LA_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGS1812603.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12790..14151)
                     /gene="dnaA"
                     /locus_tag="ACL8LA_000815"
     CDS             complement(12790..14151)
                     /gene="dnaA"
                     /locus_tag="ACL8LA_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGS1812604.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14364..15122)
                     /locus_tag="ACL8LA_000816"
     CDS             complement(14364..15122)
                     /locus_tag="ACL8LA_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGS1812605.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15180..16361)
                     /locus_tag="ACL8LA_000817"
     CDS             complement(15180..16361)
                     /locus_tag="ACL8LA_000817"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HGS1812606.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16544..17023
                     /gene="rlmH"
                     /locus_tag="ACL8LA_000818"
     CDS             16544..17023
                     /gene="rlmH"
                     /locus_tag="ACL8LA_000818"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGS1812607.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17067..17140
                     /locus_tag="ACL8LA_000819"
     tRNA            17067..17140
                     /locus_tag="ACL8LA_000819"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17101..17103,aa:Arg,seq:tct)
     gene            17305..17430
                     /gene="comC"
                     /locus_tag="ACL8LA_000820"
     CDS             17305..17430
                     /gene="comC"
                     /locus_tag="ACL8LA_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGS1812608.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            17451..18776
                     /gene="comD"
                     /locus_tag="ACL8LA_000821"
     CDS             17451..18776
                     /gene="comD"
                     /locus_tag="ACL8LA_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGS1812609.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     FKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18773..19525
                     /gene="comE"
                     /locus_tag="ACL8LA_000822"
     CDS             18773..19525
                     /gene="comE"
                     /locus_tag="ACL8LA_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGS1812610.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19577..19648
                     /locus_tag="ACL8LA_000823"
     tRNA            19577..19648
                     /locus_tag="ACL8LA_000823"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19610..19612,aa:Glu,seq:ttc)
     gene            19654..19727
                     /locus_tag="ACL8LA_000824"
     tRNA            19654..19727
                     /locus_tag="ACL8LA_000824"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19688..19690,aa:Asn,seq:gtt)
     gene            complement(19768..20310)
                     /locus_tag="ACL8LA_000825"
     CDS             complement(19768..20310)
                     /locus_tag="ACL8LA_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1812611.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDVIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20489..22092
                     /locus_tag="ACL8LA_000826"
                     /pseudo
     CDS             20489..22092
                     /locus_tag="ACL8LA_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(22122..24674)
                     /locus_tag="ACL8LA_000827"
     CDS             complement(22122..24674)
                     /locus_tag="ACL8LA_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGS1812612.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24736..26358)
                     /locus_tag="ACL8LA_000828"
     CDS             complement(24736..26358)
                     /locus_tag="ACL8LA_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1812613.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26564..27589
                     /gene="trpS"
                     /locus_tag="ACL8LA_000829"
     CDS             26564..27589
                     /gene="trpS"
                     /locus_tag="ACL8LA_000829"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGS1812614.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27741..29219
                     /gene="guaB"
                     /locus_tag="ACL8LA_000830"
     CDS             27741..29219
                     /gene="guaB"
                     /locus_tag="ACL8LA_000830"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGS1812615.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29270..30367)
                     /gene="recF"
                     /locus_tag="ACL8LA_000831"
     CDS             complement(29270..30367)
                     /gene="recF"
                     /locus_tag="ACL8LA_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGS1812616.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(30370..30738)
                     /gene="yaaA"
                     /locus_tag="ACL8LA_000832"
     CDS             complement(30370..30738)
                     /gene="yaaA"
                     /locus_tag="ACL8LA_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGS1812617.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30897..32147
                     /locus_tag="ACL8LA_000833"
     CDS             30897..32147
                     /locus_tag="ACL8LA_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS1812618.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32144..33427
                     /locus_tag="ACL8LA_000834"
     CDS             32144..33427
                     /locus_tag="ACL8LA_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS1812619.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33469..34290
                     /gene="rodZ"
                     /locus_tag="ACL8LA_000835"
     CDS             33469..34290
                     /gene="rodZ"
                     /locus_tag="ACL8LA_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGS1812620.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFIFFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34301..34846
                     /gene="pgsA"
                     /locus_tag="ACL8LA_000836"
     CDS             34301..34846
                     /gene="pgsA"
                     /locus_tag="ACL8LA_000836"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGS1812621.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34843..35670
                     /locus_tag="ACL8LA_000837"
     CDS             34843..35670
                     /locus_tag="ACL8LA_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGS1812622.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35655..36494
                     /locus_tag="ACL8LA_000838"
     CDS             35655..36494
                     /locus_tag="ACL8LA_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGS1812623.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36487..37281
                     /locus_tag="ACL8LA_000839"
     CDS             36487..37281
                     /locus_tag="ACL8LA_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1812624.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37340..38158
                     /gene="mreC"
                     /locus_tag="ACL8LA_000840"
     CDS             37340..38158
                     /gene="mreC"
                     /locus_tag="ACL8LA_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGS1812625.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38158..38652
                     /gene="mreD"
                     /locus_tag="ACL8LA_000841"
     CDS             38158..38652
                     /gene="mreD"
                     /locus_tag="ACL8LA_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGS1812626.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGVFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38746..39924
                     /gene="pcsB"
                     /locus_tag="ACL8LA_000842"
     CDS             38746..39924
                     /gene="pcsB"
                     /locus_tag="ACL8LA_000842"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGS1812627.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40148..40927
                     /gene="rpsB"
                     /locus_tag="ACL8LA_000843"
     CDS             40148..40927
                     /gene="rpsB"
                     /locus_tag="ACL8LA_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGS1812628.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            41006..42046
                     /gene="tsf"
                     /locus_tag="ACL8LA_000844"
     CDS             41006..42046
                     /gene="tsf"
                     /locus_tag="ACL8LA_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGS1812629.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42361..42615
                     /gene="tnpB"
                     /locus_tag="ACL8LA_000845"
     CDS             42361..42615
                     /gene="tnpB"
                     /locus_tag="ACL8LA_000845"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1812630.1"
                     /translation="MDSLAYVVKTHFELDSFSGQVFLFCGERKDRFKALYWDGQGFWL
                     LYKRFENGKLTWLSTEKDVKALAPEQVDWLMKGFSITPKI"
BASE COUNT        13400 a         7377 c         7890 g        14121 t
ORIGIN      
        1 ctccagcctc tctcagggtt atttttcgtc attcattttt gactttactt catcatagga
       61 tagtgcatga gattcctctt ctactgcttc aggcatcttt cctgtttcgt aaagagcttt
      121 aatttgtgta ctatccaatg tttcgtattt caataatgct tctgcaatta acttgtgagt
      181 ttcacgattt gactgaataa tttcagcagc tttatttcgt gcctcattta ataatgaacg
      241 aacctcttca tcaatttcat aagctgtttg ttctgaaatt gatttttgag gactctgtgc
      301 accaagcata gcatggtttc cttcatattg tactgggcca agtttttcac tcataccgta
      361 ctctgtaacc attgcacgtg ccatttgtgt cgcttgttca aagtcgtttg aagctcctgt
      421 agtttggaca ttaaagataa tttcttcagc tacacgtcca cccattaagc cagccaattg
      481 ctctttcata tcttctttag atagaagcat ttgatcctct ttaggaagtg caatcatgta
      541 tccgcctgca cggccgcgtg gtacaattgt aaccttatgg acaacgcgag cattcgacaa
      601 gactagacca acaatggtat gtcctgcctc atggtgagca accaattctc gttctttttg
      661 tgaaactgtc ttatctttct tagaaggtcc agcaataact ctatcttctg cttcatcaat
      721 atctgaagca tcaattatcg atttattgcg acgagcagca actaaagctg cttcattcaa
      781 gacattctct aaatcagcac caacaaagcc tggagtttgt tgagccacta atttcaaatc
      841 aacatcttct gctaaaggct tgttcttagc gtgaactttc aagattgctt cacgaccttt
      901 aacatcagga cggccaacca atacttttct atcaaaacgt cctggacgca aaagggcagg
      961 atcaagtaca tctgaacggt ttgtcgcagc gatgacgata atcccttcat ttccctcaaa
     1021 accatccatc tcaatcaaaa gttggttcaa ggtttgttca cgttcgtcat tacctccgcc
     1081 gagaccgact ccacgttgac gtccaacagc atcaatttca tcgataaaga tgatagctgg
     1141 tgctgctttt ttggcatcct caaaaagaga gcgaacacga ctagctccaa ctccgacaaa
     1201 catttctaca aagtcagaac ctgagatact aaagaatgga acacctgctt ctccagcgac
     1261 tgccttagca agcaaagtct tacctgtccc cggaggtccc tccaaaagaa cacctgctgg
     1321 aatacgggct ccaagttttg tgaatcgttt tggatctttt aagaactcaa caacttcaac
     1381 tagttcttgt ttttcttcct cagctccagc aacatctgaa aatcttactt taatatcttc
     1441 tttatttgct gctttagcct tactacgtcc aaaactcatt ggattacggc cattgcctcc
     1501 tcccatattt cccatcatag agaataggaa gaagaataga attccaaatg gcacaatgga
     1561 tacgagtaga ttaatccata taccacttga actttcatgc ttaacagtta cttctgcttt
     1621 atggtcagta gcaagttttt gcaattctga tacggtagta tctgcaggaa gaatagtgct
     1681 ggtaaatttc tctaccttag taacagatgg cgtgaaaaac tgaatacctg ttccttcttt
     1741 acttgtttta ggatttttat agacaccaga aacttcgata acactaccat ttggttggta
     1801 agttaattct tttacattac catcggtaat ttcttgtacc aactcagtat agttgatttg
     1861 ctgacttcct cctgagttat tcccagaata gaaatactgg aatcctgtca caaggaaaaa
     1921 gataaataat aaccatagaa aaggattttt aattaaacca ttattttgtt ttttcattaa
     1981 agattattct ttctaatttg aatacacttc ctctttcaat actccaatat aaggaagatt
     2041 acgataattt tctttgtagt ctaaaccata acctactaca aactcatttg ggatagtaaa
     2101 gcaggtatag tctgcctcaa tttctacaac acgtccttct ggtttatcca acaaggttgc
     2161 aattttaaca gaagctgctt ctctttcttt aaacatatct cgcaaattct tcaaagtttg
     2221 acctgtatca atgatatctt ctacaaatag aacatgtctt cctttgatat cttgagtcac
     2281 atcttgttta atattgataa caccactact tgctgttcca ccatggtagc tagaaaccat
     2341 catgaagtcc atttcaatat gtgtatcaat atgtttgacc aattcagcca taaaaggaat
     2401 agatcctttt aaaatcccaa ctaagattgg attttttcct gcatagtctt tagttaattg
     2461 agcacctagt tttttagctg cttctgtaat ttcatcgtgt gaaacgagga cttttttaat
     2521 atcgttttct aacatttttt acctatctat tttttctata taaagtacag tgttcattat
     2581 atcatttttc gtttttttac tcaaattatt ggtcgcaatt cccaaaattg agacaatttc
     2641 accaaattgc tcaataataa gagcagagtt tctcttttcc atagggattt tcaaatcaat
     2701 aaataaacgt ctgagttttt ttctatgccc attttgaatc aaaacatctc ctgtttttcg
     2761 atgacgaatg tgtatggatg tttcacgtga aacaggtatt tgttgaatta attcaccttc
     2821 taatggaagt ccaaaggaaa ataaatatcc ttgataagct acctgatttt gatagtgtaa
     2881 cacaagttca tcttcctctt catcagcctg cggactgatt ttacaaatct gaaactgttg
     2941 gtactctttt atcaattcat agccattttt aatcggatga cgatactggc ttttagattt
     3001 taaaatctgc tgaacttcag caaactgagc ttttgtaaga ttcaaatctg gaaaacgatt
     3061 cagataagtt tgaagtaaaa ctctttgtgt agactcagag taagaaaata actgctgtaa
     3121 attttccaca ttaatattgt tagataattc agctattgcc aaatcataat ctaaaatttc
     3181 attgccgatg cctaagattg catccctaaa tcgaggattt tctttttcca attctggtaa
     3241 gtaagaattt cgaatacgat ttcgaaaata atgattctcc tgatttgatg tatcttcaaa
     3301 gtgaaaaatt gatggaaagt cttttttctg aaaatgcaag aagggacgaa tgatttctat
     3361 ctctccgact acttgcttct ccttaattcc tgatagatag cgcaagcgag ttcctcgaat
     3421 caagcgcatc aaaatcgttt ccacctgatc atcagcatgg tgggcagtga ctaaagctgt
     3481 cgcacctgtc tttttcatga cctcttgaaa aaaatcataa cgaaaatttc gtgcacgcgc
     3541 ttctgaaaat tctcctgaaa aattgctgat ataaatagga agctctgctt cagcagccaa
     3601 cttccttaat tccttttctt cccaatctga ttcaattctc tgcttatgat tcacatgagc
     3661 tagaatcaat tcaatctcta actctttttg ataagtagac aataccttaa atagaaacat
     3721 ggaatctaat ccaccagaaa gagctagaac cgccttagca tgctttttga aatatccctt
     3781 cttgagaaaa tgatttaaaa aatctggttc cctcatttta gaacctcata aacatccttg
     3841 gctatcttag aaatcgtatc ataatcagaa ttcttagtga aaatagaaag aataaatgga
     3901 gaatctgcat agacaacacc cgtatcatgc ttaaattcat ccgcatctcc aattttatga
     3961 gctactttaa cagaaacacc tttggcaatt cgctgactat caaaatctgt tttagtcaaa
     4021 gactctagca caaatccatt ttgattataa atagcttcca taaacttccc ggccatctta
     4081 gaagaaatca atttttcttt tggatcccaa tcatctccca taatggcaga catcttggat
     4141 ttgaatgtgg catcagattg gtttgaaatg taatatccca atagattatg agctacatta
     4201 tcagattctt ttgatacttt cgtaattaaa tcctttaaag aatattcttt attatcttct
     4261 tttttaggaa gactaccact tccctctggt ttataagaac ctggaaaatc attgactgca
     4321 gatacgtatt ttacagtcgt atctaactga taaagaccct catttatttt ttcttgcgta
     4381 taatagagat aggagagttt caaaacgctg gctgcataca tcttttcatc ttgattgata
     4441 ccagcttctt ttccagtagt cagttgctta acataaatag agaaagaatc tttctgatat
     4501 ttttcagata acatttcttg aactttactc atccgattat cttcttcaga agttgattct
     4561 ttagctaccc atccagcctg atcaatatgt agaaattctc ttccttctac aaacatggtc
     4621 ttgtcgattg atacttgcga ataagctgat aaggatgatt tcacttcttt taaatcataa
     4681 ggactattgt acagtttaaa gtcagattct aaccatgctt tttttattgt tggagttacc
     4741 tctgattgat catataaaaa tcgtttgtcc gcagctataa attgatgatt tgatagctta
     4801 aatactggaa ttccttgttt atttaagcgc cactcggtta tttgaaaact tgttttggga
     4861 gtcaatttcc cagattccac tactaaatct tcattcgcat aaacaggaac ctctccataa
     4921 accatgggag aacttaattt ttctctaaaa taaatactaa agtcagattg tgaaaggtaa
     4981 taaatttctt tcgaagtata gacgacttct ttttctgtgc taacgacttt tgaaatggtc
     5041 aaaaaacttg gtagcaacaa aataattaag aatttacgca ttcttccttt ccttttcttc
     5101 ttgtaaacgc aataattgat ttttatcagc caattcttcc agcttttcat ctgacaaact
     5161 caaaaattgc tctattacgt ctaataaatt ttccatttta tcacctttga agcaagtcag
     5221 gaatcgtata aactttttcc ctcgacttag aataatagta cttcgctcgt gtatatttag
     5281 cagcataatc ttcatctttc aacttggtag caaatgctgt ctccttatcc ttttcatcac
     5341 tcaaagtttg atactgagtt tgcaagtctg ctaattgctg acgtctttgg agtaattgct
     5401 gataactctg cgctaaatta aaagttggca agataaataa tagcataatc aaaatcaata
     5461 cccctcccat aaaacgattc cgtttttgtc gttctttcat caggtagcga cgacgttggt
     5521 attcattttg aataaaagaa ttattcaatt gtacaatatt tttagacatt ttcttctacc
     5581 cgtgtttcac tgataatttc atacattcct gctgcatctt ctttttttgt actatctttc
     5641 atctctagta cttttacaag cagcaactta ttgccaaagc gaatttcaac ttggtcatta
     5701 actttcaagt ccgttgaact tttggccaag attccattaa ccttgattct acctttatct
     5761 gctacttcct ttgcgactgt acgacgcttg ataattcgcg atacttttaa atatttatct
     5821 aatctcatcc ttattacctc aatttattat tgtaccattt ttatctattt tatagaagaa
     5881 aaatatcaaa tggaattttc ttccttaaac tcttttatct ctaataaact ttctccaaaa
     5941 atcagcagac cttctaaaat ttcataatct ttcttatttt ggacatcaaa tacaagctcc
     6001 attaatccct tattctcagc gatgcctgct tttaagttcg ttacggataa agctttaaaa
     6061 taatcttgag ctaaaaacag ccgttgagtg actttttcaa attgaattgt aattttatta
     6121 tcttttcttt ccacacgttg aacaaagacc ttgtccaagt atgatttaac caaaccaatc
     6181 tctaaaagat aggctactac atctgggtat tctccaaaac ggtctatcaa ctcctcttgt
     6241 aactcttcat aattgacacg gttgtcaatt tgacgaattt tcttgtaaat ttcaatctta
     6301 tgtcgttgat cagaaatata agtatcagga agataggcat caatttgcaa aatcaactca
     6361 gcattccctt tggttcttgt gttagcgtta gcattaccgt ttcgtttagc aatagcttcc
     6421 tctaataact gcgaatacaa ttcaaaacca acagaatcaa tgaaaccaga ctgggatttt
     6481 cctaaaagat ttcctgctcc acgaatcgaa agatctcgca ttgcaatctt aaagccagag
     6541 cccaattctg taaatccttt aatcgcttct aatctctttt cagagacttc actgattgat
     6601 ttttctggac gatacatgag ataagcataa gcaatacgat tactacgacc gactcttcct
     6661 cttaactgat ataaggttga caagcccata tggtccgcat tttcaataaa taaagtatta
     6721 gcatttggaa tgtccacccc tgtctcaata atagtagtcg tcaccaagat atcgtattgt
     6781 ccctcaataa agtctaatag agtattttcc aactggactt cactcattcg accatgaaca
     6841 tatccaatcg aagcctccgg aatcaactcc tgtaattctg aaaccttctg aacaattgtg
     6901 tcaactttgt tgtaaagata ataaacttga cctccacgct ccatttcacg caagacagca
     6961 tcacgaatga cactatcatt cttttccaaa acataggtct gaacaggata gcgattagtc
     7021 ggcggagttt caataacaga taaatctctg attcccagca tagacatatg gagggtacga
     7081 gggattggcg tagcggtcaa ggttaggaca tccacttgtt tcttcagttc tttcaaagtt
     7141 tccttatgct tgacaccaaa tcgctgttcc tcatcaataa tcatcaagcc caaatcagca
     7201 aacacaacat cttttgacaa aacacgatgt gttccaatca aaatatcgac ttgaccgttt
     7261 ttcaattttt caagtgttgc agtctgctct tttttacttc taaagcgact caacacatca
     7321 atattaactg caaaattttg gaatcgttcc ttaaaattcg tatagtgctg ttgcgctaaa
     7381 accgtcgtcg gaactagaat gacaacctgt ttgtgatcat tgactgcttt aaaggctgca
     7441 cgcatagcaa cttcagtctt tccaaaacca acatccccaa ctaaaagtcg atccattggc
     7501 tgagaagcct gcatatccct cttgatttcc tcaatactac gaagttgatc atccgtttca
     7561 acataaggga aagcatcatc aaaggcatct tgatcatcat catcagctga gaaagcaaaa
     7621 cccttcaact gactacgttc agagtagagt ttgattaaat catcagctat atcctctacc
     7681 tggttcttaa ccttttgctt ggccttttta aaatgaccgt catttaattt attgagtttt
     7741 ggagctttac catcacttga aatatatttg gacagtagat gaatctgttc cacggggata
     7801 gaaatttgat caccattttg gtattggaca ctgacataat cgcgatgaat tcccttgatt
     7861 tcaatggttt caattcctag atattgacca atcccatgga tatgatggac aacatagtcc
     7921 cctttttcaa gttcattgta atcttttaat ctctctgcat ttgaaacatg ttgtcttcga
     7981 aaacgacgct ttaatttctt ttgaaaaatc tcatgttcag ttatcaataa aatcttttca
     8041 tctacaaaat gaaaaccatg tctgagatta ccctctatta agtttacaga ttctttacag
     8101 atatttgtct tatctctaga atccaattta atctgatatt cctctaacat atcctccaat
     8161 gttttacttc ccattgaatt gctagactgc agaataatgg tgtaatccat ttttttatat
     8221 cgttcaattt cttcttttag aaaagaaaac tgattgaaaa attcctgcat aggatattga
     8281 ttgaattgat aaattttgtc aaatttgaga tttcctaaac ccttttgaag attagagaaa
     8341 aaggtcactg gactttgttt tttatagatt tgttcaatat cagaaaaata ctgcatatca
     8401 gaaaatgctt tactattctg taattcttct gtaaagtact gcgctaagtc tctttcaaag
     8461 acttcatact gattcatcaa tttttgataa tcatcaaaga atattggggt atctttttca
     8521 atataatcaa agacagtcca tgtcttatca tagcacaaag ataaaaactt ccgagagtct
     8581 gcatgacttt gtttttggtg aaaacttgaa agaatttctt ctaggtatga tttcaaaata
     8641 ggtgataaag tttttgaaat ttgtttttct aaagctgact gtcctcgttg ataatccttt
     8701 tctctcaaaa gcatatcact agctggaaag atagtgagtt ctgtcttatt ttctttcgat
     8761 aattgtgttt ctacttcaaa tgacctgata ccatcaattt catcaccaaa aaactcaatt
     8821 cgacaaggtt ctaactggga tatttcaaaa atatctaaaa tatctcctcg aagactaaat
     8881 tcgccctgag tttgtacttg agtaactttt cgatagccat tttcctttaa ctgatggata
     8941 aacgcgtgtt gatcatattc ttcaccaact gagattttta caatactatc tttgaatgca
     9001 ttgggagacg gtaaaatcaa tcgacttgct acgatattac aaactaaaat ccctttctta
     9061 gatgaatcag tcaaaaaacg caaggcttca acccgtgaaa taattttttc ctgtgaagac
     9121 atcaaaaact ccaccatagg agcatcatct accaaaaatg gatagacgag ttcctcaccc
     9181 aagatagata taagatcact aacaagtcct tctgcttctc cataagttga cgtcaataac
     9241 acaatcctat cttctttttc taaactgctt gcaattgcaa gagccttagt agatgttgat
     9301 aaaccaagta ttagttgtct tttcttatct gttaaatttt gatgccattt tttaatctga
     9361 tcattttctg agaataaatc taataaggtc accatttatc cgttatacct ctgcattgtt
     9421 ttctcaaaat ttttctcttg taaatagtag tttacagaat cgtcaacttt gtcaatagac
     9481 tgtaaaatac cgatataatc atccctgtca aacttactca aaacatgatg aacaactgac
     9541 ataccatttt taggtcttcc aattccaatc ttaacacggt taaagacctg agttcctata
     9601 tgttgaataa tagacttgat accattatga ccacctgctg agccttttgc tcttaaacga
     9661 attttcccaa cttccatgtc aagatcatcg taaatgataa gtaaatcgtc aatatccaaa
     9721 ccatagtaag ttaataaagc atgaactgct tttccacttt cattcataaa ggtcgttggt
     9781 ttaaccagat aaattttttc tccatttagg aaaaaggatg ctaggtcagc ttgaaatatc
     9841 ttatcgtgtg taaaagtgac attctgtttc ttcgctagtt gatcaatcaa cataaaacca
     9901 acattgtgtt ttgtttcaaa atatttatcc cctggatttc ccaagcctac aagtaatttg
     9961 gtcatttatt tttcctttca aaagccaaaa gggttggaat tttttttcca acctaattga
    10021 caccatttat taatttttag acattaaagc ggaattccat gatatcgcca tcttgaacga
    10081 tatattcttt tccttcttca cgcaagcgtc cagcttcttt tacggccttt tcagatccgt
    10141 atttcactag atcttcatat gacatggtta ctgcacgaat aaagcctttt tcaaagtctg
    10201 agtggataat accagctgct tgaggagcct tcataccacg tttgaaagtc caagcgcgaa
    10261 cttctttttc accagctgtg aagtaagttc ccaatccaag caagtggtaa gctgcacgcg
    10321 tcaacttatc tacacctgat tctgtcaaac caatggcttc aagaaactct tttttatctt
    10381 catcatccaa ttcagaaatt tcttcctcag cacgcgcaga aataacgact acttcagcat
    10441 tttctgtcgc tgcaaattca cgaatttgtt tgacatagtc gatagagtca ggttctgaaa
    10501 ccacatcctc gtccacatta gctacataaa gaactggttt agtcgtcaaa aggaaaagac
    10561 ctttgacaac cttttgttcc tcatctgtaa attcaatggt acgagctgat ttcccgtctt
    10621 ctaggactgg tttaatcttt tgaagaacat tgaattctgc tactgattct ttatcttttt
    10681 gcgtacgtgc catcttttct acacgcgcat atcgtttgtt cactgattct aagtcagcaa
    10741 gaatcaattc cagattaatt gtatcaatat ctgcaagtgg atctacaaag gcgtcttcac
    10801 gtccttgctc gcgcatcaca ttttcatcat caaaagcacg aactacgtga acaatcgcat
    10861 ctacttcacg aatattggcc aagaatttat tccctagccc ttctcctttt gaagctcctt
    10921 ttacaatccc tgcaatatct gtaaattcaa atgttgtggg aactgtcttt ttaggagtta
    10981 tcatttcagt tagtttttgt aggcgttcat ctggaacttc caccattcca acatttggat
    11041 caatagtcgc aaatgggtag tttgctgcct ctgctcctgc ttttgtaatt gcattaaata
    11101 atgttgattt accaacgttt ggcaaaccaa cgatacctgc tgtcaaagcc atatttctca
    11161 ttctccgttt tcatttcaat ccctaatatt ataacacaaa aagggataaa gtgctaaccc
    11221 tctaactaag ggttctcagt caataatttt attcattttt cgctcaaaat catatcgccc
    11281 catcatgaca acatgctcac aattactgca ttttattttg atatctgctc ctacacgtgt
    11341 aacttcccaa cgattagcct ttttaccagt cgacttgatt gtacaagcgt gtgatttttt
    11401 catctcaaca aaatttccaa cttgatacat actactctcc ttttcttttt cgattatatc
    11461 ataaaagagg cgagccaggc tcaacctctt tcacttaatt tgtacgaact ggtgtaatga
    11521 gctgcatgaa gtcttcgtca gtatctgctg gcacaagagt aaatggacga acagctgaga
    11581 taaagctaat ggtcaccttt tcgctattta aagctttaag agaatcaatc aagtaagttg
    11641 ggttgaaact aatggtcaaa tcttcaccag taacctgatc agtatcgatt tcttcgttta
    11701 ctttaccaac ttctggagag tgaacatggg cgctaacaac cccatcctta atttcaagtt
    11761 tcacagtacc attttgagtc gcacttgata aaagacgggc acgctccatt gactggcgta
    11821 agtttaccac attaaaagta atagtagtgt taaagtctgt tggaatcaag cgatctgtat
    11881 caggatagtt tccttctagg agacgagtat agaagctaat attttcgctt ctaaagagga
    11941 tttggttatt ggcaaagaaa atctctacag tttcgatatc atctgtaaat accgctgaaa
    12001 attcgcgtag agaacggcta ggaattacga catcaaaatc atcactattt ttttcaagag
    12061 tcaatttttt ctggcttagg cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt
    12121 gactcaatac gaagtggaca cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa
    12181 aggctgtttc attaataatt ttcttgagta attttgtttc aagtattaaa ggagtgcttg
    12241 ctgaaatttc ttggattcgt ggatattgtt cgctatcttt tccttttagg gtaatttctg
    12301 atttgccact ggttaaaaca atttgatttt gttcaatttc tttaaaatca agagtcacat
    12361 caggtaaact agatactaca ttgataaaga aagaagcttc aagaaggatc gaacctaaag
    12421 aagtaattaa caaaccagca tcttcatttt tttgagaaat aaaattttca attgaaattt
    12481 gaccatttga accaattaaa gtaatacctt cattggtcac gtcaattttt actgttgata
    12541 aaataggaat ggcattttta gaactaatag ctctcttagt agtatttaat gcttgtagaa
    12601 ataaattttt attaattgaa aaatgaatca tggattctcc tttatttatt tttagtatta
    12661 gaaagataat agtaataata gagtctgtga aatctgtgga aaacagcctt taaacaagtc
    12721 atatcaagtt ttttagcttg tttaaaaaat gtggataaaa aagataaaaa actaaaagtt
    12781 atccacaaat tatttgattt tctttttgat tgattcaatt tctaaacgta aattatcgtc
    12841 ttgatcaatc aaagatttta ttttggcatg ggcatgaatg actgtggtat gatcttttcc
    12901 cccaaattcc ttcccaattt ttggaagact attatctgtt agttctctag ataaatacat
    12961 ggctacttga cgggccaaaa caatattttg aaggcgtcta cttcccttca tttctttgat
    13021 actaacacca taaaagttac caacttcagt ttggatttta tcaattggga tgacgagcat
    13081 ttggctaaca tcttgtttgc gggctctaat ggcttctgca gcaatatcaa tagtgatatc
    13141 cttgattttt tttactctgg caattaaagt gatgtcgttg atggctccct caagatctcg
    13201 aacatttgaa tcaaattgcc cagctaggta ttctagagta tcactttgga aattgtagcc
    13261 taaatgttcc gtcttacttt gtaaaatggc aatacgtgtt tcaaagtcag ggggtgtgat
    13321 agtttgtgtc aatccccaac taaaacgcgt gacaagcctc tcctcgagcc cttctagatg
    13381 ttttggacta cgatcactcg ttaggacaat ctgtttttgc ttgtcatgaa gggcgttaaa
    13441 ggtattgaaa aattcttcct gagttgcgac tttttttccg ctgagtgact gtatatcatc
    13501 gattaacaaa agatcaagac tacgataggt ctttttaaac ttttccattt ccccaagtct
    13561 taggtgatca agaaagtcat taataaagct ttcggcaggg atatatttaa cacgcgcatt
    13621 aggaatattt tttagaattt catttccaat agcgtttaat aagtgagtct taccaaggcc
    13681 tggtcctcca tagataaaaa gagggttata ggtcagagcc aaatcttcag agacagctaa
    13741 agcggctgat acagcccaaa catttccatc cccttgaata aagttatcaa aggtatactt
    13801 ttcttttaat cccgtatctg aataaggaat agatactaac tttggactat agtcataaag
    13861 agttaaattt gtagcttctt caacttgtga gctagtcgta tcttgaggtt tggtgaaaat
    13921 atagtgggga gttatttcag cgtcataaat ttcaaaacca gctactacaa taatatcttt
    13981 tagttgtttt tcccagacca tttccatttc agagcgaggt agaaatatag tggcaacatt
    14041 ttcctctacc ttgatgagtt cagcttgaat agcatagaaa tcatacatgg atcgagtcag
    14101 tctttcttgt gcaaattcta atatacgatt ccaaaattgt ttttctttca atcctttctc
    14161 ctcctttact atttaaaagt ttaatactag agatatttta ccatagatag caagaatttt
    14221 ccacaagctg tgaaaaataa tccacaatgt taccaaactt tatccacagg ttggggataa
    14281 aagaagaaat tattgattta ttaagctttt tatcgaaaaa catagtggat aaccttgtga
    14341 gataaaaaaa taaaagaaca gccttatttc aggctgttga taattctact atattcttct
    14401 tgatttgaaa aagaaataat gatttttcca ctgtcttttt tagatagttt aatttctaca
    14461 tctaatccga gtagttttct taactgtttt tcttcatttt gtatgaaatg attagttttt
    14521 tgctgtttct tttgtttttt ctctgtcaga agagtttcta acttccttac agaaatatct
    14581 tcttctataa tccgttgaaa gaaatagtct tgttgttcct tatttaaccc aactagggaa
    14641 cgcgcatggg cttgtgatag tttgccattt tctacttctg agagaatctg ttctggcaag
    14701 gaaagtaaac gaatggagtt gctgatatat ggacgagact tgcccatctt atctgcaatt
    14761 tcagcatggg tgaatccttt ctctacgaga gattcatagg cgcgtgcttc ttctattggg
    14821 tttaaatttt ctctctgtaa attttcaata atggactgga ccatcatctc ttggtctgaa
    14881 atctgtttaa caacagctgg gatagaccgt agaccagcta aaagtgaagc ccgatagcgt
    14941 ctctctcctg caaggatttc ataaccaata acaggagatt gacgaacaat aatcggttga
    15001 atgaccccat tttctttgat agactgtgct agttcatcta gcttttctct atcaaattct
    15061 tttcggggtt gatagggatt tttttgtata tctgtgatag aaatcatttc aaatttttcc
    15121 atgattctac actaacacat cttttctctt atgtaaagct ttctttacat agatgtcaat
    15181 taagattcta aatcacctga actcttgtta agtttgatag aggtagtttc ttctttcccg
    15241 ttacgatagt aggttatctt aatggtgtct ccgatagaat ggttgtaaag agcactttgt
    15301 aagtctgttg atgaagcaat ctctttgtca tctacttttg taattacatc gtatttttca
    15361 aggtgaccat tggcaggcat attactttgt accgaacgaa caactacacc agatgtaaca
    15421 ttacttggaa tattgagtct tctgatgtcg cttgtactca cattagataa attaaccatc
    15481 tggattccca aagctggacg cgtcactttt ccgttttttt ctaactgttc aataatattg
    15541 atagcatcat ttgcaggaat tgcgaaacca agaccttcta cagatgttcc tccatttgta
    15601 gcaattttac ttgaggtaat tccgataacc tgcccttgaa tattgatcag tgggccgcca
    15661 gagttacctg ggttaatagc agtatcagtt tggatggctt ttgtagaaat agcttgtcca
    15721 tcttccgatt ttaaggatac atttctattg agactggata cgataccttg agtgacagta
    15781 tttgcatatt cagaacctaa cgggctaccg atggcaatag cagtttctcc tacagttaac
    15841 ttactagaat caccaaactc agctactgtt gtcacttttt ctgaagagat ttttacgaca
    15901 gcaatatcag agaaagtgtc agctccgaca atttctccag gtactttagt cccatctgac
    15961 aatcgaatat ctactttgct ggcgccattt ataacgtgat tgttggtgac gatgtaagct
    16021 tctttatcat tctttttata aataactcca gatccttcac tagagattcg ctgagaatct
    16081 gtgtcagtat catcattgcc aaatacgcta ttttgtctgt ttgccgaata agtaataaca
    16141 gaaacaacag catcttttac tttgttaaca gcctgtgttg ttgaattttc gttcttatag
    16201 gcagtttgtg taatagtact attattgtta gagttgttta cactactttt ttgagttagt
    16261 tgagttattg aaaaactacc caaggctcca ctaaaaaagc taatgacgat aacgactaat
    16321 aattgaaacc attttttgta aaatgttttt agatgtttca tatttgcctc catatgtttg
    16381 aattactgaa agtataaact gactagctta attataactt aaacacaaaa gttttacaca
    16441 aactgtggat aactcttttg aaactgtgat tttcttaatt gaaatctatt ttttattttg
    16501 tgaataagat gtgaaaaaat agagaatatg ttagaataga gtcatgaaaa ttaaagttgt
    16561 aacagttggg aaactgaaag aaaagtattt aaaagatggt atcgcagagt attcaaaacg
    16621 aatttctaga tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa caccagataa
    16681 ggccagtgaa tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa ttttatcaaa
    16741 aattgctgac cgtgatttcg ttattgtgtt agccattgaa gggaaaactt tcttctcaga
    16801 agaatttagt aagcagttag aagaaacttc tataaaagga ttttctactc ttacttttat
    16861 tattggggga agtttaggat tgtcatcatc tgtaaaaaat agagccaatc tttctgtcag
    16921 ttttggtcgc ctaaccttgc ctcatcagtt aatgagacta gttcttgttg aacaaatcta
    16981 tcgcgctttt acgattcagc agggattccc ctaccataaa tagaaaattg acttttaatt
    17041 gaatttttgg tagaataatt gtgttaggtc tcatagctca gctggataga gcattcgcct
    17101 tctaagcgaa cggtcgcagg ttcgaatcct gctgggatca atataatagc aaagctggga
    17161 attttcccgg cttttttctt aaaaaagtac actttgggag aaaaaaatga cagttgagag
    17221 aattttatct aaagtgaaat tccattttgt ataatggttt ttgtaagtta gcttacaaga
    17281 aaaaacattt taggagattt tattatgaaa aacacagtta aattggaaca gtttgtagct
    17341 ttgaaggaaa aagacttaca aaatattaaa ggtggggaga tgaggatttc aagaatcatc
    17401 cttgattttc ttttcctacg aaaaaagtaa tgaaataagg agaaagagta atggatttac
    17461 ttggatttgg gacagttatt gttcattttt taattattag tcacagttac cgtttaattt
    17521 gtaaaggtcg aataaataga aaagaattat acatttttgg tgcttataca ttactaactg
    17581 aaatagtact tgaattttcc ttttatcttc tatatttaga taaaataggg attgaaagat
    17641 ttttatttcc tttgggctta tattcctatt ttcgatggat gaaacagtat gagagggata
    17701 gaggactatt cctaagttta ctactatctc ttttatatga gagcactcat aactttctgt
    17761 ccgtaacttt ctcctctata acaggagata attttgtttt acaatatcat ttcccattct
    17821 ttttcgttgt gacggtgtta acctattttg ttacattaaa aatcatttat tatttccatt
    17881 tggaactagc ctattttgac aaggactacc tttatccttt cttgaaaaaa gtattttttg
    17941 ctttactatt gctacatatt gtatctttcg tttcagatat ggtaagtacg attaaacatt
    18001 tgaatagttt tggaagtatt ttgtcatcta ttgtctttat ctctctcctt ttgaccttct
    18061 ttgcaatgaa ttctcataaa gttcaaatgg agaaagagat tgcttttaag cagaagaaat
    18121 ttgaacagaa acatttacag aattacacag atgaaattgt tggtctgtat aatgaaatcc
    18181 gtggttttcg acatgattat gctggaatgc ttgtcagcat gcagatggca attgacagtg
    18241 gtaatttaca ggaaattgac agaatttaca atgaagtttt agtcaaagca aatcataaat
    18301 tgcgttcaga taagtacact tactttgatt tgaacaacat agaagactca gctttacgaa
    18361 gtttggttgc tcagtcaatt gtctatgctc gaaataatgg tgtagagttt acactggaag
    18421 taaaagatac gattaccaag cttccaattg aactattgga tttggttcgt atcatgagcg
    18481 ttttattgaa taatgctgtc gaaggatcgg ctgatagcta taaaaagcag atggaagtag
    18541 cagttattaa gatggaaact gaaacagtta tggtgattca gaattcatgt aaaatgacga
    18601 tgactccttc aggagatcta tttgccttag gattctccac taagggaaga aatcgcggag
    18661 tcggattaaa taatgtgaaa gaactactag ataagtacaa caatattatt ttagaaacag
    18721 agatggaagg cagtacattt agacaaatca ttagatttaa gagggaattt gaatgaaagt
    18781 tttaatttta gaagatgtta ttgaacatca agtgagacta gagagaatat tggatgaaat
    18841 ttcgaaagaa tcgaatattc caatatcata caagacaacg ggaaaagtcc gtgaatttga
    18901 agaatacatt gaaaatgatg aagtaaatca gctttatttc ctagatatcg atattcatgg
    18961 aattgagaaa aagggatttg aagtggctca gctcattcgt cattacaatc cttacgctat
    19021 tattgtcttt atcactagtc gatcagagtt tgcgactcta acctataaat accaggtatc
    19081 agccctagat tttgttgata aggatatcaa tgatgagatg tttaagaaga gaattgagca
    19141 aaatatcttc tacacgaaga gtatgttact tgaaaatgaa gatgttgtag attatttcga
    19201 ctacaattac aagggaaatg atttaaaaat tccttaccat gatattttgt atattgaaac
    19261 aacaggggta tctcataaat tgcgcattat tggtaagaat tttgcaaaag agttttatgg
    19321 taccatgaca gatattcagg aaaaggacaa acatactcag cgattttatt ctcctcacaa
    19381 gtcatttttg gtaaatatag gcaatatcag agaaattgat cgaaaaaact tagaaattgt
    19441 tttctatgaa gaccatcgtt gtcctatttc aagattaaaa attagaaaat taaaagatat
    19501 tttagagaaa aaatctcaaa agtgattgac aattagcaag aaattgatat aatggttata
    19561 tctgctacag atagaaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt
    19621 aacacgggtt cgaatcccgt acggactatt ttatccgcca tagctcagtt ggtagtagcg
    19681 catgactgtt aatcatgatg tcgtaggttc gagtcctact ggcggagtat agataaaagg
    19741 gaacacagct gtgttcctct ttttgtatca atttgtatca ccaagcattt tcataaggaa
    19801 gtctgttatt tcttgagaac tttctttttt tccatgtgca atccaagttt ggcagacacc
    19861 aaaaagtgca tgagttagat agatgctact atattctaat tcagtggtat ttagattcag
    19921 ttgcataaat cgcttttgta aatctatact gagcatgata tgaagtttat ttcgtaagaa
    19981 attttggatt tctttagtcc cattttcaga aagaagggca gccagaagtg gttctgactc
    20041 tagatattca aagacttcta aaatagcgtc tcttttgtga tgagcatgtt tttgaaaaat
    20101 atattcaaat gtatggaata gcttgctttg atagtgctca atcacatcat acttatcctt
    20161 atagtgagta tagaagctgg aacgactaat tccggctttt tctactaatt tgacagtaga
    20221 aattttatca aatggctgtt ccatcagtaa ttgtaccata gcattttcaa tagttcgctt
    20281 tgtttttaag cgtttgttac tttcttgcat atttcctcct tgtaaacaaa ttagactata
    20341 tgtctaaaaa tagatttttt atcttgtaat ttagattttt taatgtataa tctattatat
    20401 taaaatttta gacaatatgt ttaaaaaagg agaaactaag tttaaagaat ggaaagcaat
    20461 ttaaaaaaaa ccaaccttta ttattgtcat gatcgggatt tctcttattc cagatctgta
    20521 caatatcata tttttgtcat caatgtggga ttcatatggg caattgtctg acttacctgt
    20581 ggcagttgta aataatgata aagaggcttc ctataatggt aatactatgg caataggaaa
    20641 agacatggtg tccaatttaa aagaaaataa aaccttggat tttcattttg tagatgaaga
    20701 ggaaggaaag aagggattgg aagatggcga ttactatatg gtagtgactt taccaagtga
    20761 tttatctgaa aaaacaacta cattatccaa tattcaatcg acagcagctt atcaatcatt
    20821 gacaagtgag caacaaactg agataagtga ttctgtatct caaaattcaa ctgatagtat
    20881 tcaatcggct cagtcaattg tagctttagt acaagattta cagggaagtt tagaaaactt
    20941 acaaaatcaa tcttctaatc tttcgacttt aaaaaatcaa tctaatcaag tatcacctat
    21001 tacttctact tctttgatag gattgtcaag tggattaaca gagatacaag gagatgttac
    21061 tagcaaatta gttcctgcca gtcagtcgat tgcatcaggt gtaaacgcat atactacagg
    21121 tgttgataaa gtttctcagg gcgcaagtca actaagtgaa aaaaatgcca ccttgacagg
    21181 tagtttggat aaactagttt caggctcaaa caccttgaca caaaaatctt ctagattgac
    21241 agcaggagtt ggttaattac aatcaggatc tgggcaatta gcagacaaat ccagtcagtt
    21301 actttcaggt gcttctccat tagagaatag agctaataaa ttggcagatg gatctgggaa
    21361 actagcagaa ggtggaacaa agttaacttc tggattggaa gatttacaga caggacttgc
    21421 ttctttagga caaggactag gtaatgctag tgatcaactc aaatcagtat caacagaatc
    21481 taaaaatgca gagattttgt caaatccact caatctttca aaaacagaca atgatcaagt
    21541 tcctgtaaat ggaatcgcaa tagctcctta tatgatatca gttgctcttt ttgttgcagc
    21601 aatatcaaca aatatgatat ttgcgaaatt gccttcagga cgtcatccag agagccgttg
    21661 ggcttggttg aaatcttgag ctgaaataaa tggtattata gctgttttgg caggaatttt
    21721 ggtatatgga ggagttcagc ttattggttt aactgctaat catgagatga gaatatttat
    21781 tctcatcatc ctaacaagtt tagtattcat gtctatggtg accactttag caacgtggaa
    21841 tagccgtata ggagcttttt tctcacttat tttgctttta ctacagttag catcaagtgc
    21901 aggtacttat ccacttgctt tgacaaatga tttctttaga tctattaatc cctggttacc
    21961 aatgagctat tcagtttcgg gattacgaca aacaatctct atcaacaagt cattttccta
    22021 gctgtcatac tagttctatt tactagttta ggtatgctag cctatcaaca taagaaaatg
    22081 gaagaagatt aaaaaaatcg accgattaac tggtcgattt tttatgcctt agatgacttt
    22141 cgtctgtgat tatagattcc aaatagtaag agagaagtaa aggaacagat tgctccagta
    22201 ataaaaccat tgggaatgaa ggaaagtgta atagttcctt tccccttggg aatgtcaact
    22261 ttcataaatc cagtttgagc ttgtttaatt tctattttct taccatcttg gtaggcagac
    22321 caacctttgt cataaggaat ggtgaagaaa atagatgtat cttgttggac atcatatgta
    22381 gcaaaaacct tgtttttaga agttgatact gtgacaggtt gttctttaat tttttgaatt
    22441 gcctcggtga aagttttggt atctaaacga tagaaggtag gagattcaaa tgatacttgt
    22501 gaatttccag ggaaactaac attgatattg aaagtttttt tctctttagt atatcctaga
    22561 ttaaagaagg agaagacatt atcagttgta aaagtctttt tttcaccatt tacaaggatg
    22621 tcaaccttct tttgtttatc gttagaaaag tgaaggttta tgaaagagag ataaacttgg
    22681 ctgttttctg gaacttcaat ttgatactgg attgctgcat cttcatttga agaacttgtg
    22741 acactaatca aatcattagt attttctatt ttttctgttt tttcataagg tattggagaa
    22801 aaataatcaa aattgacgtt agcaagttga tttaaaaatg aggcctgatt atccaaggta
    22861 tgttcattga acttgacatc attgtaaaca gattgactag caactgcaat cggaagagag
    22921 tattgatttt catatagggt aagattatct ttttgataga tatctttaaa gccatactta
    22981 tcaataggac tgtctgagat attgtactgg ataccaaata aactatcagc caaaatacta
    23041 ttatttgcat atcggagatt gagattagtc ccagaggatt taaaaccaag tttatctaaa
    23101 gtagagcttg atgaacgatt tcgaacagat gaaaattgag agattccatt gtagttgaat
    23161 ttcatactgt catttcctgt ctgagtttgt agtttttcag tacgagtaaa ttgatttcca
    23221 atatatgttg agaaagattc catagctggg atatctcgac tataagcact tcgagaagca
    23281 aatccccatt ccttagcaat tccgtccatt tgagatgaag catttaaact catttcaacc
    23341 agtataaata aagagattag aatggcaaat agattcacag atataaactt tttgataact
    23401 gcaaggagta aaagagaata gacaaccaaa aattcaagag taagcagaat attcaaatct
    23461 gttaaaaaag aataatgcga ttttagatag atggtagcta aaaatcctgt tactacaaga
    23521 aaaagcgaaa ctaaaaaatt ccagacttta agttctttca gacgctttaa gacttctgct
    23581 gctgtgtaaa ttaacaaggt agagaaaatc caagcatagc gatgtaaaaa catgtttgga
    23641 gtatgcatgc cttgccaaaa taagtcaaga gcttctatgt aaaagcttgc aattagaaat
    23701 gcaaagaata ttacatatat gagtttcacg tgaaacttaa tagatttcag cgtaaaaaat
    23761 aaaatggtca aaataaaggg aaatagtcca acaaaaatca ttgggatggc cccatacttt
    23821 gttgtgtcaa aggaaccaat gaattgctta gcaaagagat caagatacca gctactttca
    23881 gtttgaaact ttgtaacttc agtcaatttt tccccatgtg tctgtaaatc aaatagagtg
    23941 ggaagagtca taatcaaact agccatacca gctaaaaagg agataactat gaaatcaaga
    24001 acagatgatt ttcgagtctt aaagtcccac gaaatttgac agagatacca gaaaataaga
    24061 aacaatactg tcatatatcc aaaataataa ttttgaataa ataagattga cagacttgta
    24121 aagtacaata ggagtttctt ttcagttatc agtagatgta aaccagttat aattaaagga
    24181 atcaagataa aaacatctag ccaggttttt atctctaatt gactgacagt gaaactcatc
    24241 agagcatagg aagtagataa ggctagtttt aaagtctgag ggatagattg aaacaattta
    24301 ttcaaactaa aaaaggttga cagaccaatc aatccaaatt ttaagagagt ggtcagatag
    24361 acagcatctg gcatattcgt tagatcaaaa aagtaaacta gaggcgcgag aaaactaccc
    24421 aagtaataac tagatagggc atagaagttt agccctagac cacttgtaaa ggtgtaaaac
    24481 agactactat ttccatgtag gatatttcgt aaggctacat caaaaataac gtattgatga
    24541 aagccatctc ctaatagagg agagttgtcg ctattccagt agataccttg agatagatat
    24601 actccagaca taatcactac aggaatgatg aaagaaataa aataggttcg atatgttttt
    24661 aaaaatgatt tcatgttacc tcgtagaatg atagaaaact cagttggtta acccaactga
    24721 gttttgaagt tttatttagt ctttccaaag ttctttaact tttgcttgta cttctgcatt
    24781 ttctaggaat tcatcgtagg tttcatcgat acggtcaatg acgccatttt tagataagac
    24841 aatgatatgg ttagccaaag tttgaataaa ctcgtggtca tggctggcaa agatgattga
    24901 ttctttaaag tttttcaatc catcattcaa gcttgagata gattccaagt ccaagtgatt
    24961 tgttggatca tcaagtacaa ggacatttga ttttaagagc atgagttttg aaagcatgac
    25021 acgaactttt tctccccctg acaagacatt tacaggtttg ttaacttcat ctccagagaa
    25081 gagcatacgg ccgaggaaac cacgtaggaa agtattgtca tcatcttctt tacttgcgaa
    25141 ttgacgcaac cagtcaagaa ttgattctcc tcctgcaaaa tcagctgagt tatcttttgg
    25201 taggtaagat tgactagttg taactcccca cttgacagtt ccttcatagt caatatctcc
    25261 catgattgca cgaattaatg cagtcgtttg aatatcattt tgtccaataa gtgctgtctt
    25321 atcatctgga cgcaagatga aactaatatt atccaagata gtttcaccat caatctttac
    25381 agttagattt tctactgtca agagatcatt accaatctca cgttccgctt taaagttgat
    25441 aaatggatat ttacgactag atggtacaat ctcttctagc tcaatcttat caagcatttt
    25501 cttacgtgat gttgcctgcc ttgacttaga agcattggca gagaaacgag caacgaattc
    25561 ttgcaattgt ttaatttttt cttctgcttt agcattacgg tctgctagca atttagcagc
    25621 aagctcagaa gattccttcc agaagtcgta gtttccgaca tagagtttga tttttccaaa
    25681 gtcaaggtcg gccatgtgag tacaaacttt gtttaagaag tgacggtcgt gggatactac
    25741 gataactgtg ttatcaaagt caattaagaa gtcttctaac caagtaatcg attggatatc
    25801 caaaccgtta gtaggctcgt ccaagagaag aacatctggt ttaccaaaaa gtgctttggc
    25861 gaggagaacc tttacttttt caccgttggc caattcgctc atgttttggt agtgtaattc
    25921 ttctggaatg tttaggtttt gaagtagttg agaggcttca ctctctgctt cccaacctcc
    25981 aagttcggca aactctcctt cgagttcggc agcacgaacc ccgtcctcgt ctgagaaatc
    26041 ttccttcatg tagatagcat ctttctcttt catgatgcta taaagttttt catttcccat
    26101 gataacgaca tcaatggcac gttcatcttc gtagtcaaag tgattttgac gaagaacaga
    26161 gagacgttca tctggaccaa gagagatgtg accagtagta ggttcgatat ctccagctaa
    26221 aatttttaaa aaggttgatt ttccggcacc attagcaccg attaatccgt aagtatttcc
    26281 ttctgtaaat ttgatattga catcatcaaa aagtttgcga tcactaaaac gtagtgaaac
    26341 atcagatact gtaagcaata tttttctcct atatgtgtaa tatatttatt ctactagaaa
    26401 atacagaaat attcaaattt ttatttttca attttgtgta aattatattt acagtatcct
    26461 ttacacaaat ctgtaaaaag caaggctgat ttattttgat aaattacggt tatttcatta
    26521 aaaaaatgct ataattgaaa ggactatatc gaaggagaac aaaatgacta aacccattat
    26581 tttaacagga gaccgtccaa caggaaaatt gcatattgga cattatgttg gaagtctcaa
    26641 aaatcgagta ttattacagg aagaggataa gtatgatatg tttgtgttct tggctgacca
    26701 acaagccttg acagatcatg ccaaagatcc tcaaaccatt gtagagtcta tcggaaatgt
    26761 ggctttggat tatcttgcag ttggattgga tccaaataag tcaactattt ttattcaaag
    26821 ccagattcca gagttggctg agttgtctat gtattatatg aatctagttt cgttagcacg
    26881 tttggagcga aatccaacag tcaagacaga gatttctcag aaaggatttg gagaaagcat
    26941 tccgacagga ttcttggtct atccaatcgc tcaagcagct gatatcacag ctttcaaggc
    27001 taattatgtt cctgttggga cagatcagaa accaatgatt gagcaaactc gtgaaattgt
    27061 tcgttctttt aacaatgcat ataactgtga tgtcttggta gagccggaag gtatttatcc
    27121 agaaaatgag agagcagggc gtttgcctgg tttagatgga aatgctaaaa tgtctaaatc
    27181 actaaataat ggtatttatt tagctgatga tgcggatact ttgcgtaaaa aagtaatgag
    27241 tatgtataca gatccagatc atatccgcgt tgaggatcca ggtaagattg agggaaatat
    27301 ggttttccat tatctagatg tttttggtcg tccagaagat gctcaagaaa ttgctgatat
    27361 gaaagaacgt tatcaacgag gtggtcttgg tgatgtgaag accaagcgtt atctacttga
    27421 aatattagaa cgtgaactgg gtcctattcg tgagcgccgt attgaatttg ctaaggatat
    27481 gggagaagtt tataatatga ttcaaaaagg tagtgaaaga gcgcgtgaag ttgcgggtca
    27541 aaccctatct gaggtaaaag gggcaatggg acttcattac tttaactaag tttattttac
    27601 aagaaactat cttttctatc tcgaagaaaa gatagttttt tatttacccc tgtaacattt
    27661 gacaaatttt tatagaatgg tatgatagat acaatataaa aagagtcaag ctcaaaaaga
    27721 aagaaaagag gaaacttcga atgtctaatt gggacactaa atttttgaaa aaaggtttta
    27781 ccttcgatga tgtattgctt attccagctg aaagtcatgt gttgcctaac gatgcagatt
    27841 taacaactaa attggcagat aatttgactt taaatatccc aattattacc gctgccatgg
    27901 acacagttac agagagtcaa atggccattg ctattgctcg tgcaggcggt ctcggagtta
    27961 tccataaaaa catgtcaatt gctcaacaag cagacgaggt tcgtaaggta aaacgttctg
    28021 aaaatggagt tattattgat ccgttcttct tgacgcctga acatacaatt gctgaagcag
    28081 atgagcttat gggtcgttac cgcatcagtg gtgttccagt tgttgaaaca cttgaaaatc
    28141 gtaaattggt tggtattttg acaaaccgag atcttcgttt tatttcagat tataatcaac
    28201 caatttcaaa ccatatgact agtgaaaatc ttgttactgc tcctgtgggt acggatcttg
    28261 caacggctga gagtattctt caagagcatc gtattgaaaa acttccgttg gtcgatgaag
    28321 aaggcagtct ttctggtttg atcactatca aagatattga aaaagttatt gagtttccaa
    28381 atgcggctaa agatgagttt ggtcgtcttc tagttgcagg tgcagtaggt gttacttcag
    28441 atacatttga acgtgcagag gctctttttg aggcaggagc ggatgcgatt gttattgata
    28501 ctgcacatgg tcattctgca ggtgtcttgc gtaaaattgc cgagattcgt gctcatttcc
    28561 cagatcggac tttgattgct ggaaatattg ctactgctga aggtgcacgt gccctttatg
    28621 aagcgggtgt agacgttgtt aaggttggta ttggaccagg ttctatctgt actactcgtg
    28681 tgattgctgg tgttggtgtt ccgcaagtaa cagctatcta cgatgctgca gctgttgcgc
    28741 gcgaatatgg taaaacgatt attgctgacg gtgggatcaa gtattctgga gatattgtaa
    28801 aagcacttgc tgcaggtgga aatgctgtta tgcttggatc tatgtttgct ggaactgatg
    28861 aagctccagg cgaaactgaa atcttccaag gacgtaaatt caagacttac cgtggtatgg
    28921 gatcaattgc tgctatgaag aaaggttcaa gcgaccgtta tttccaaggt tctgtcaatg
    28981 aagcaaacaa gcttgttcca gaaggaattg aaggtcgtgt tgcttataaa ggagcggcag
    29041 ctgatattgt tttccaaatg attggtggta ttcgctctgg tatgggttac tgtggtgcag
    29101 ctaaccttaa agaactacac gataatgctc aatttattga aatgtctggt gctggtttga
    29161 aagaaagcca tcctcatgat gtgcaaatta ctaatgaggc accaaattat tctatgtaaa
    29221 aaacaatgaa aagaactcca gtgaaaacag gagttctttt acaatgttgt caatttccat
    29281 ttacagaagc tttaccatcc tgaatagtga agatacttag attttctggc agattttgaa
    29341 gatggtctaa gcttgttgtt gtgataaagg tttggattga ttgagaaatc gtttctaata
    29401 attttaactg tctagtgttg tcaagttcac tcatcacatc gtcaagcagt aatatcggag
    29461 attctgtggt aatgctttcc attaattcga tttctgctaa ttttatcgag aggacgagac
    29521 tacgatgttg accttggctt ccgaaactag catccatccc atttatataa aaagaaatgt
    29581 catctcgatg aggaccgaca ccagtattct ttttaaataa atctctggat ctactttttt
    29641 ctaaagcaat tttgaaagat tcggataagt tttgtttgtc agttatattg acagaagatt
    29701 gataggatat tgacaactct tcgatctgat tagagagttc aaaatgtttc ttacgcccaa
    29761 atgattctag tttttttatg aaatctaagc ggtgattcat tacacgacat ccataatcaa
    29821 ctagctgatc atctaacaca gaaaggaatg tttcatctat tttttgatct gattttaggt
    29881 aagtgtttct ttgctttagg atgtggttat aattggttaa gtcagataag tagattggct
    29941 taatttgccc aagttccata tcaatgaatt ttcgtcgaat cgaaggtgct cctttaatta
    30001 gttgtaaatc ttcaggagca aataagacaa cattcatgtg tcctacataa tctgaaaggc
    30061 gtgcctgttt taagtgatta acttttgtta cacggccttt ttgtgttagt tcgatttcta
    30121 ggggaatgga tccagttttt ttctgaacga gacctgaaag atgaagttgt tcctcatcaa
    30181 aatgaatgag atttttatct gttcgagttc gatgactacg cgttaaggct aaaaaataga
    30241 tagcctctaa catgtttgtt tttccttgtg cattacgtcc caaaaagaca ttcaatttag
    30301 gattaaagtc tattttcgtt tctttgtaat tacgaaaagt cttgagagat agatgttgta
    30361 gccacatgat tatctaccag ggaatcgtgg agcttgttgg cttttaggtg atgaagcagg
    30421 tttcgatttg tcttttttaa ctcccttatt catctttttg acaagtttag cgatccgttc
    30481 tttttcaact ttatcagctt ggtattcatc ttgttcttca gaagtaggtt gtgtcaacaa
    30541 gatgtcaatg ttcatgtcag ggatgtcaac tttatcgcca atacgaagtt tttttccacg
    30601 acgactctct aattccctat taaagtaaac agaatgttca gagagaaatg atttaatagc
    30661 tcctccgcta tgtgtaattc caagttcttt gagtagtgct tggagggtaa taaattcttc
    30721 aaataatttg tattccataa ttcacctcaa tctttggtat tataccatat tttcctaaaa
    30781 atagtgagag agacagcgag aaatgtaagc gatttttatt tcaaaagtgt tacagggaac
    30841 aagaaaattt caggttttcg tgatataata gaagtctgta tataaggagg taaatcatgg
    30901 agttagtgca tggaatttca acacatttta tccaatcaaa aaagtttaaa acaaacaaaa
    30961 ttaccgtgcg ttttaccgct ccattatccc ttgatacgat tgcaggtcac atgttgagtg
    31021 caagtatgct agagactgct aatcagatgt accccacttc tcaagatttg aggagacact
    31081 tggccagtct atacggtaca gatatgtcaa ccaattgttt cagaagaggg caaagccaca
    31141 ttatagaatt gacatttacc tatgttcgtg atgagttttt aagtaggaaa aacgtgctaa
    31201 cctctcagat tttggaactt gtaaaagaaa ctcttttttc acccgtagta gttgataatg
    31261 ggtttgatcc ggccttattt gaaattgaga aaaaacaatt gctagcaagt ttagcagctg
    31321 atatggatga ttctttttat tttgcacata aagaattgga taaattgttt tttcatgatg
    31381 aacgtcttca attggaatat agtgatttac gaaatcgtat tttagctgaa actccacaaa
    31441 gttcttattc ttgtttccaa gaatttttag ccaatgatcg aatagatttc tttttcctag
    31501 gtgattttaa tgaggttgaa attcaaaatg tattagaatc atttggcttt aaaggtcgaa
    31561 aaggagatgt gaaggttcag tattgtcaac cttattctaa tatccttcag gaaggtatgg
    31621 ttcggaaaaa tgtgggacaa tccattttgg aattaggtta tcattactgt tctaaatatg
    31681 gtgatgagca acatttaccc atgattgtaa tgaatggttt acttggtgga tttgctcact
    31741 ctaagctctt tacaaatgtc cgtgaaaatg ctggattagc ttataccatt tcaagtgagc
    31801 ttgatttatt tagtggattc ttgaggatgt atgctggtat caatcgagaa aatcgtaacc
    31861 aggctcgtaa aatgatgaat aatcaactgc ttgatttaaa aaaaggttat tttacagagt
    31921 ttgagttaaa tcagaccaag gaaatgattc gttggtcgtt gttactttct caagataatc
    31981 aatcttcatt gattgaacgt gcttatcaaa atgccttatt tggaaaatct tcagcagact
    32041 ttaaaagttg gattgcaaag cttgaacaga ttgacaaaga tgctatttgt agagtagcta
    32101 ataatgtgaa actacaagcg atttacttta tggaaggaat agaatgacaa aggttgtttt
    32161 tgaagaaaaa tactatccag ctgtaaaaga aaaggtttat cgaactcgtt tggccaacgg
    32221 attgacagtt gctcttttgc ctaaaaagga atttaaagag gtttacggga gtgtaactgt
    32281 acagtttggt tcgatagata cgcttgtcac agaagttgac ggagatgtaa aacaatatcc
    32341 tggaggaatt gctcattttc ttgaacataa attatttgag agagaagatt ctagtgattt
    32401 gatgtcggct tttacgagtc taggtgcaga tagtaatgcc tttacaagct ttacaaaaac
    32461 aaactatctt ttttcagcaa cggattattt tttagaaaat ttatatttac ttgatgaatt
    32521 ggtaacatca gcacacttta ctgaagcttc cattctgaca gagcaggata ttattcagca
    32581 agaacgagaa atgtaccaag atgatccaga ttcgtgttta ttcttttcaa ctttagcgaa
    32641 tttgtatcct ggtacacctt tagcaactga tatagttgga agtgaggagt ccatttccca
    32701 aatcaatcta actaatttgc aagaaaattt tacaaagttt tacaaacctg taaacatgtc
    32761 tctgttttta gttggtaatt ttgatgtgga gcgagtacag gactattttg aaagcaaaga
    32821 actgaaagat tcagattttc aggaagtagc aagagaaaag ttgtttttac agcctgtaaa
    32881 gccaacagat agtatgagaa tggaagtatc ttctcccaaa ctagcgattg gagttagagg
    32941 taagcgagaa gtttctgaag cggattgcta tcgacatcat attttattaa aattattgtt
    33001 tgcaatgatg tttggttgga cttcggatcg ttttcaaaaa tgttatgaat caggtaaaat
    33061 tgatgcgtcc ttatctctgg aagttgaaat aacaagtcgc tttcattttg tcatgttgac
    33121 aatggatacg aaagagccag ttgctttgtc tcatcaattt aggaaggcta ttcgtaattt
    33181 tacaaaggat ttagatatta cagaggaaca tttagatatt atcaaaagag agatgtttgg
    33241 cgaatttttc agtagcatga actctcttga atttattgca acgcaatatg atgcttttga
    33301 aaatggtgag acaatttttg atttgccgaa aattttacag gaaattactt tagaggatgt
    33361 ccttgatgct ggacatcatt taatagatga tggtgacata gttgatttta caatattccc
    33421 atcgtagtaa cctattataa tagacactag aaagaaggga tgacaagtat gagaaaaaaa
    33481 acaattggag aggttttacg attagctaga atcaatcagg gattgagttt agatgaattg
    33541 cagaaaaaga cagaaatcca gttagatatg ttggaagcaa tggaagcaga cgatttcgat
    33601 caacttccaa gtccttttta cacgcgttct ttcttgaaaa aatatgcatg ggctgttgag
    33661 ttagatgacc aaattgtttt ggatgcttat gattctggga gtatgattac ttatgaggaa
    33721 gtagatgttg atgaagatga gttgacaggt cgtagacgtt caagtaagaa aaagaagaaa
    33781 aaaatatcat ttttaccttt attttatttt atcttttttg ctttatcgat tttaattttt
    33841 gtgacttatt atgtttggaa ctatattcaa actcaaccag aggagccttc tctttctaat
    33901 tacagtgtgg ttcaatcaac aagttcaact agctctgttc cccactcctc aagtagtagt
    33961 tcttctagta tagaatcagc tataagtgta tcaggcgaag gaaatcatgt agaaatcgct
    34021 tataagacaa gtaaggaaac agttaaattg caattggcag tttcagatgt tacaagttgg
    34081 gtcagtgttt cagaaagcga acttgagggc ggtgtaacct tatcgccaaa gaagaaaagt
    34141 gcagaagcaa cagttgcaac taaaagtcct gtaacaatta cgttaggtgt tgtaaaaggt
    34201 gttgatttga cagtagataa tcagactgtt gatttatcga aattaacagc tcagactgga
    34261 caaatcactg taacctttac taaaaattaa ggaaaaacga atgaaaaaag aacaaattcc
    34321 caatctctta acaataggtc gaattctctt tatacctatt tttatcttta ttttaacgat
    34381 aggaaattcg atagagagtc atatagttgc agctattatc tttgctgttg ccagtattac
    34441 cgactattta gatggatatt tagctcgtaa atggaatgtg gtcagtaatt ttggtaaatt
    34501 tgcagatcct atggcggata agttactagt tatgtcggct tttattatgt tgattgagtt
    34561 aggtatggct ccggcttgga ttgttgcagt gattatctgt cgtgagttag ctgtgacagg
    34621 tttaaggctt ttattggttg aaactggtgg aacaatttta gcagcagcaa tgcctggaaa
    34681 aattaaaact tttagtcaga tgtttgccat tattttcttg ctattacatt ggactttgct
    34741 tggtcaagtt ctactttatg tagccttatt tttcactatc tactctggct atgactattt
    34801 caagggtagt gcctatgtat ttaaagggac atttggttcg aaatgaaatc aataattgat
    34861 gtaaaaaatc tttcttttcg ctataaagaa aatcagaact actacgatgt gaaggatatt
    34921 acgtttcacg tgaaacgtgg agaatggctt tcgattgtag ggcataatgg tagtggtaaa
    34981 tcaacgacgg ttcgattaat tgatggctta ctggaagcag aatccggaga gattgtaatt
    35041 gatggccaac gtttgactga ggaaaatgtt tggaatatac gtcgtcaaat cggtatggtt
    35101 tttcaaaatc cagacaatca atttgttgga gcgactgttg aagatgatgt tgcttttggt
    35161 ttggaaaatc agggactttc tcgtcaagaa atgaaaaaga gagtggaaga agctctggct
    35221 ttagttggca tgttggactt taaaaagaga gagccagcgc gtctatcagg tggccaaaag
    35281 caacgtgtgg ccattgcagg tgttgtagcc ctaagaccag ctattttaat cttagatgaa
    35341 gcaacgagta tgttggatcc tgaggggcgt agagaactta ttgggacagt aaaaggaatt
    35401 cgaaaagact atgatatgac ggtcatttct attacccatg atttggaaga agtcgccatg
    35461 agtgatcgcg tattggtcat gaaaaaaggg gaaattgaat caactagtag tccaagggag
    35521 cttttctctc gaaatgattt agatcaaatt ggattagacg atccttttgc taatcaatta
    35581 aaaaaatctt tgagccagaa tggctatgat ttacctgaaa attatttgac agaaagtgag
    35641 ctagaggata agctatggga attgctctag aaaatgtgaa ttttacatat caagaaggta
    35701 ctcccttagc ttcagcagct ttgtcggatg tttctttgac gattgaagat ggctcttata
    35761 cagctttaat tgggcacaca ggtagtggta aatcaactat tttacaactc ttaaatggtt
    35821 tattggtgcc aagtcaaggg agtgtgaggg tttttgatac cttaatcacc tcgacttcta
    35881 aaaataaaga tattcgtcaa attagaaaac aggttggctt ggtatttcag tttgctgaaa
    35941 atcagatttt tgaagaaacg gttttgaagg acgttgcttt tggaccgcaa aattttggag
    36001 tttctgaaga agatgctgtg aagactgcgc gtgagaaact ggctctggtt ggaattgatg
    36061 aatcactttt tgatcgtagt ccgtttgagc tgtcaggggg acaaatgaga cgtgttgcca
    36121 ttgcaggcat acttgccatg gagccagcta tattagtctt agatgagcca acagctggtc
    36181 tagatcctct agggagaaaa gagttgatga ccctgttcaa aaaactccac cagtcaggga
    36241 tgaccatcgt cttggtaacg catttgatgg atgatgttgc tgaatatgcg aatcaagtct
    36301 atgtaatgga aaagggacgt ttagtaaagg ggggcaaacc aagtgatgtc tttcaagacg
    36361 ttgtttttat ggaagaagtt cagttgggag tacctaaaat tacggccttt tgtaaacgat
    36421 tggctgatag aggcgtgtca tttaaacgat taccgattaa gatagaggag ttcaaggagt
    36481 cgctaaatgg atagtatgat tttagggcgt tatatcccag gggattcgat tgttcaccga
    36541 ttggatccac gtagcaaatt gcttgctatg atgctactga ttttgattgt gttttgggct
    36601 aataacccct tgacgaatct gattcttttt atagcgacag ggatatttat tgccttgtca
    36661 ggagtatctc tttcattttt tattcagggc ttgaagtcta tgtttttctt gattgccttc
    36721 acaactattt ttcaactatt tttcatttct aatgggaatg ttttatttga gttttcgttt
    36781 gtgagaatca cggattatgc tttgcaacaa gctgggatta ttttttgtcg ctttgtattg
    36841 attattttct tttcaacttt gttaacctta acaaccatgc ctttaagttt ggcatcagct
    36901 gtcgaagctt tattagcacc tttaaagcgt gtgaaagttc cagttcatga aattggattg
    36961 atgctgtcta tgagtttgcg ttttgtccca accttgatgg atgatacgac tcggattatg
    37021 aatgcacaga aagctcgtgg agtggatttt ggagaaggaa gcatcgttca aaaagtaaag
    37081 gcgatgattc ccattttgat tcctcttttt gcgacaagtt taaaacgtgc agattccttg
    37141 gctatcgcta tggaagcgcg tggctatcag ggtggaaaag gcagaagtca ataccgacaa
    37201 ttgaaatgga ctctgaagga tacgctgacc attcttgtta ttctcgtact tggttgttgt
    37261 ttatttttct taaaatctta gtagctttca ggaattgata aaaagttact gtaacagttt
    37321 ttgatataaa ggtaggaata tgaaccgttt taaaaaatca aaatatgtca ttattgtttt
    37381 tgtcactgtt ctgcttgtgt cagctctctt agcgacgact tattcaagta caattgtgac
    37441 aaaattagga gatggaatct cattggttga tagagttgta caaaaacctt ttcagtggtt
    37501 tgattctgtc aaatcagatt tggctcattt gacacgaaca tataatgaaa atgaaagttt
    37561 gaagaaacag ctttaccaat tagaagttaa atcaaatgag gtggaaagtt taaagacaga
    37621 aaatgaacaa ctgcgccaat tgcttgatat gaagtctaaa ttgcaagcca caaagacttt
    37681 agcagcagat gttattatgc gttctccggt atcttggaag caggagttga ccttagatgc
    37741 aggtagatca aaaggtgctt ctgagaacat gttagctatt gcaaatggtg gcttgattgg
    37801 gagtgtttca aaagtagagg agaactctac tatagtcaac cttctgacaa atacggaaaa
    37861 tgctgataag atttctgtta aaattcaaca tggctctact acaatttatg gaattattat
    37921 tggctatgac aaggaaaatg acgttcttaa aattagccaa ttaaatagta atagcgatat
    37981 tagtgcggga gataaggtga ctactggtgg attaggaaac tttaacgttg ctgatattcc
    38041 tgttggtgaa gtggttgcca caacgcatag tacagactat ttgacacgag aagtaactgt
    38101 taaattgagt gcagatactc ataatgtaga tgtgatagaa ttagtgggga attcataatg
    38161 agacagttga agcgagttgg agtattttta ttgcttcctt tctttgttct aattgacgcc
    38221 catattagcc agcttctggg ctcatttttc ccccatgtac atttggctag tcattttctt
    38281 tttctatttc tcttatttga gacgatagaa gtatcagagt atctctacct agtctattgt
    38341 tttgttatag gcttggttta tgatgtttac tttttccatc taatagggat tacaactctc
    38401 ttatttatct tattgggagt cttccttcat aaattgaata gtgttatttt gttgaatcgt
    38461 tggacaagaa tgctagctat gattgtgctg acattcctgt ttgaaatggg tagttatctt
    38521 ttggctttta tggtagggtt gacagtagat agcatgtcga tttttatagt ctatagcttg
    38581 gtaccgacga tgattttaaa ttttttatgg attactgttt ttcaatttat ttttgaaaaa
    38641 tattatctat aagaacgaca tataaatgta acaaaggcgt aatatttatt aggccttttt
    38701 ttggtatact agtattgtct ttaaaagaag gagtatctac gtaatatgaa gaaaaaaatc
    38761 ttagcgtcac ttttattaag tacagtaatg gtttctcaag tagctgtttt aacaactgcg
    38821 catgcagaaa cgactgatga caaaattgct gctcaagata ataaaattag taacttaaca
    38881 gcacaacaac aagaagccca aaaacaagtt gaccaaattc aggagcaagt atcagctatt
    38941 caagctgagc agtctaactt gcaagctgaa aatgatagat tacaagcaga atctaagaaa
    39001 ctcgagggtg agattacaga actttctaaa aacattgttt ctcgtaacca atcgttggaa
    39061 aaacaagctc gtagtgctca aacaaatgga gccgtaacta gctatatcaa taccattgta
    39121 aactcaaaat caattacaga agctatttca cgtgttgctg caatgagtga aatcgtatct
    39181 gcaaacaaca aaatgttaga acaacaaaag gcagataaaa aagctatttc tgaaaaacaa
    39241 gtagcaaata atgatgctat caatactgta attgctaatc aacaaaaatt ggctgatgat
    39301 gctcaagcat tgactacgaa acaggcagaa ctaaaagctg ctgaattaag tcttgctgct
    39361 gagaaagcga cagctgaagg ggaaaaagca agtctattag agcaaaaagc agcagctgag
    39421 gcagaggctc gtgcagctgc ggtagcagaa gcagcttata aagaaaaacg agctagccaa
    39481 caacaatcag tacttgcttc agcaaacact aacttaacag ctcaagtgca agcagtatct
    39541 gaatctgcag cagcacctgt ccgtgcaaaa gttcgtccaa catacagtac aaacgcttca
    39601 agttatccaa ttggagaatg tacatgggga gtaaaaacat tggcaccttg ggctggagac
    39661 tactggggta atggagcaca gtgggctaca agtgcagcag cagcaggttt ccgtacaggt
    39721 tcaacacctc aagttggagc aattgcatgt tggaatgatg gtggatatgg tcacgtagcg
    39781 gttgttacag ctgttgaatc aacaacacgt atccaagtat cagaatcaaa ttatgcaggt
    39841 aatcgtacaa ttggaaatca ccgtggatgg ttcaatccaa caacaacttc tgaaggtttt
    39901 gttacatata tttatgcaga ttaatttaca gagggactcg aatagagccc tcttttcagg
    39961 ttttaccgtg acaatcccta ttaaaaatta tatcaaaata gcttgaaaat attggaaaag
    40021 tatggtagaa tgaaaattgt cgtgtgaacg ataatactca ttcttgatga attgtgaagc
    40081 agttgccctt gggtcgtttt gcgagttgaa gtcaagaaga ggaaaaaaac aaaaaggaga
    40141 aatactcatg gcagtaattt caatgaaaca acttcttgag gctggtgtac actttggtca
    40201 ccaaactcgt cgctggaatc ctaagatggc taagtacatc tttactgaac gtaacggaat
    40261 ccacgttatc gacttgcaac aaactgtaaa atacgctgac caagcatacg acttcatgcg
    40321 tgatgcagca gctaacgatg cagttgtatt gttcgttggt actaagaaac aagcagctga
    40381 tgcagttgct gaagaagcag tacgttcagg tcaatacttc atcaaccacc gttggttggg
    40441 tggaactctt acaaactggg gaacaatcca aaaacgtatc gctcgtttga aagaaattaa
    40501 acgtatggaa gaagatggaa ctttcgaagt tcttcctaag aaagaagttg cacttcttaa
    40561 caaacaacgt gcgcgtcttg aaaaattctt gggcggtatc gaagatatgc ctcgtatccc
    40621 agatgtgatg tacgtagttg acccacataa agagcaaatc gctgttaaag aagctaaaaa
    40681 attgggaatc ccagttgtag cgatggttga caccaatact gatccagatg atatcgatgt
    40741 aatcatccca gctaacgatg acgctatccg tgctgttaaa ttgatcacag ctaaattggc
    40801 tgacgctatt atcgaaggac gtcaaggtga ggatgcagta gcagttgaag cagaatttgc
    40861 agcttcagaa actcaagcag attcaattga agaaatcgtt gaagttgtag aaggcgacaa
    40921 cgcttaattt atacaaatag taattaccta ggagggcggg gcttagcccg gctctcctat
    40981 tttcaaaaaa tataggagaa ttaaaatggc agaaattaca gctaaacttg taaaagagtt
    41041 gcgtgaaaaa tctggtgccg gtgttatgga cgctaaaaaa gcgcttgtag aaacagacgg
    41101 tgacatcgaa aaagcgattg aattgcttcg tgaaaaaggt atggctaagg cagctaagaa
    41161 agctgaccgt gttgctgcag aaggtttgac tggtgtttat gttaacggta atgttgcagc
    41221 agttattgaa gtaaacgctg aaactgactt cgttgcaaaa aacgctcaat tcgttgaatt
    41281 ggtaaatact acagctaaag tcattgctga aggaaaacct gctaacaacg aagaagctct
    41341 tgctttgata atgccttcag gtgaaactct tgaagctgca tacgtatctg caacagcaac
    41401 tatcggagag aaaatctcat tccgtcgctt tgcattgatt gaaaaaacag acgcacaaca
    41461 ctttggagca taccaacata acggtggacg tatcggtgtt atttcagttg ttgaaggtgg
    41521 agacgaagca cttgctaaac aattgtcaat gcacatcgca gcgatgaaac caacagttct
    41581 ttcttacaaa gaattggatg agcaattcgt taaagatgag ttggcacaat tgaatcacgt
    41641 tatcgaccaa gacaacgaaa gccgtgcaat ggttaataaa ccagctcttc cacacttgaa
    41701 gtatggatca aaagctcaat taactgatga tgttattgct caagctgaag ctgacatcaa
    41761 agctgaattg gctgcagaag gtaaaccaga aaaaatctgg gacaaaatta ttccaggtaa
    41821 aatggatcgc ttcatgcttg ataacactaa agttgaccaa gcttacacac ttcttgcaca
    41881 agtttacatc atggatgaca gcaagacagt tgaagcatac cttgaatcag ttaacgcttc
    41941 ggtagttgag tttgctcgct ttgaagttgg tgaaggtatc gagaaagctg caaacgactt
    42001 cgaagctgaa gttgcagcta caatggcagc agccttgaat aactaataat agaaaggaag
    42061 caaattgctt ccttttttgt atagggctta gatttttcct gaaattgttt tttccccagc
    42121 ctcactcgat tagaataatt aaaacaaaaa gccctataat aagggctaag tgcatttagg
    42181 agactatact tcaaattcat agtaagcgcg tcataacaag gtatctatcc taattcccca
    42241 acttccagta tactagataa aaaataatta tcggaggaaa ggtattgtac tatgataatt
    42301 caactaagtg atttaggtca agttcacctt gtttgtgaca agacagatat gaggtaggga
    42361 atagactcat tagcctatgt agttaaaacc cactttgaat tggattcttt ctccggtcaa
    42421 gtctttctct tttgtggtga acgtaaagac cgctttaaag ccctttactg ggatggtcaa
    42481 ggattttggc tactatataa acgctttgag aacggaaaac tgacttggct aagtacagaa
    42541 aaggatgtca aagctctcgc acctgaacaa gtagattggc ttatgaaggg cttttctatc
    42601 actccaaaaa tatagtagat tgaaactaga atagtacacc tctgcttcta aaacattgtt
    42661 agaaatcgat ttgattgtcc tgatcgattt gtcctgttct tatttcattt tactataaat
    42721 ccatcagaaa gttgtgattt ctattgaaat gaggactttc tttttacact catctgcttt
    42781 caaaaagc
//
LOCUS       DBERYS010000014        39985 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.014, whole genome shotgun sequence.
ACCESSION   DBERYS010000014 DBERYS010000000
VERSION     DBERYS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.014"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            78..151
                     /locus_tag="ACL8LA_000846"
     tRNA            78..151
                     /locus_tag="ACL8LA_000846"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:112..114,aa:Met,seq:cat)
     gene            155..227
                     /locus_tag="ACL8LA_000847"
     tRNA            155..227
                     /locus_tag="ACL8LA_000847"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:188..190,aa:Phe,seq:gaa)
     gene            240..320
                     /locus_tag="ACL8LA_000848"
     tRNA            240..320
                     /locus_tag="ACL8LA_000848"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Tyr,seq:gta)
     gene            325..395
                     /locus_tag="ACL8LA_000849"
     tRNA            325..395
                     /locus_tag="ACL8LA_000849"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Trp,seq:cca)
     gene            410..482
                     /locus_tag="ACL8LA_000850"
     tRNA            410..482
                     /locus_tag="ACL8LA_000850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:His,seq:gtg)
     gene            496..567
                     /locus_tag="ACL8LA_000851"
     tRNA            496..567
                     /locus_tag="ACL8LA_000851"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:528..530,aa:Gln,seq:ttg)
     gene            577..660
                     /locus_tag="ACL8LA_000852"
     tRNA            577..660
                     /locus_tag="ACL8LA_000852"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:611..613,aa:Leu,seq:caa)
     gene            767..2044
                     /locus_tag="ACL8LA_000853"
     CDS             767..2044
                     /locus_tag="ACL8LA_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGS1812631.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2120..3604
                     /gene="thrC"
                     /locus_tag="ACL8LA_000854"
     CDS             2120..3604
                     /gene="thrC"
                     /locus_tag="ACL8LA_000854"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGS1812632.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3665..4945
                     /locus_tag="ACL8LA_000855"
     CDS             3665..4945
                     /locus_tag="ACL8LA_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS1812633.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4949..5569
                     /locus_tag="ACL8LA_000856"
     CDS             4949..5569
                     /locus_tag="ACL8LA_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1812634.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5735..6847
                     /locus_tag="ACL8LA_000857"
     CDS             5735..6847
                     /locus_tag="ACL8LA_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS1812635.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7170..7595
                     /locus_tag="ACL8LA_000858"
     CDS             7170..7595
                     /locus_tag="ACL8LA_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS1812636.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7606..7965
                     /locus_tag="ACL8LA_000859"
     CDS             7606..7965
                     /locus_tag="ACL8LA_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGS1812637.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8072..8716
                     /gene="pcp"
                     /locus_tag="ACL8LA_000860"
     CDS             8072..8716
                     /gene="pcp"
                     /locus_tag="ACL8LA_000860"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS1812638.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(8744..9601)
                     /locus_tag="ACL8LA_000861"
     CDS             complement(8744..9601)
                     /locus_tag="ACL8LA_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGS1812639.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9731..10873
                     /gene="tgt"
                     /locus_tag="ACL8LA_000862"
     CDS             9731..10873
                     /gene="tgt"
                     /locus_tag="ACL8LA_000862"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGS1812640.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10971..12788
                     /locus_tag="ACL8LA_000863"
     CDS             10971..12788
                     /locus_tag="ACL8LA_000863"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGS1812641.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12941..14092
                     /gene="nagA"
                     /locus_tag="ACL8LA_000864"
     CDS             12941..14092
                     /gene="nagA"
                     /locus_tag="ACL8LA_000864"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGS1812642.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14255..15313
                     /locus_tag="ACL8LA_000865"
     CDS             14255..15313
                     /locus_tag="ACL8LA_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGS1812643.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15464..15829
                     /locus_tag="ACL8LA_000866"
     CDS             15464..15829
                     /locus_tag="ACL8LA_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGS1812644.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15905..16846
                     /gene="comGA"
                     /locus_tag="ACL8LA_000867"
     CDS             15905..16846
                     /gene="comGA"
                     /locus_tag="ACL8LA_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGS1812645.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16794..17810
                     /gene="comGB"
                     /locus_tag="ACL8LA_000868"
     CDS             16794..17810
                     /gene="comGB"
                     /locus_tag="ACL8LA_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGS1812646.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17812..18138
                     /gene="comGC"
                     /locus_tag="ACL8LA_000869"
     CDS             17812..18138
                     /gene="comGC"
                     /locus_tag="ACL8LA_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HGS1812647.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18131..18535
                     /gene="comGD"
                     /locus_tag="ACL8LA_000870"
     CDS             18131..18535
                     /gene="comGD"
                     /locus_tag="ACL8LA_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGS1812648.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18498..18800
                     /gene="comGE"
                     /locus_tag="ACL8LA_000871"
     CDS             18498..18800
                     /gene="comGE"
                     /locus_tag="ACL8LA_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGS1812649.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18763..19224
                     /gene="comGF"
                     /locus_tag="ACL8LA_000872"
     CDS             18763..19224
                     /gene="comGF"
                     /locus_tag="ACL8LA_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGS1812650.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19202..19648
                     /gene="comGG"
                     /locus_tag="ACL8LA_000873"
     CDS             19202..19648
                     /gene="comGG"
                     /locus_tag="ACL8LA_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGS1812651.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            19751..20338
                     /locus_tag="ACL8LA_000874"
     CDS             19751..20338
                     /locus_tag="ACL8LA_000874"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1812652.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20399..21352
                     /locus_tag="ACL8LA_000875"
     CDS             20399..21352
                     /locus_tag="ACL8LA_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1812653.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21403..22593
                     /locus_tag="ACL8LA_000876"
     CDS             21403..22593
                     /locus_tag="ACL8LA_000876"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGS1812654.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYFAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22594..22725
                     /locus_tag="ACL8LA_000877"
     CDS             22594..22725
                     /locus_tag="ACL8LA_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812655.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            22742..23113
                     /gene="rnpA"
                     /locus_tag="ACL8LA_000878"
     CDS             22742..23113
                     /gene="rnpA"
                     /locus_tag="ACL8LA_000878"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGS1812656.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23088..23912
                     /locus_tag="ACL8LA_000879"
     CDS             23088..23912
                     /locus_tag="ACL8LA_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS1812657.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23931..24917
                     /gene="jag"
                     /locus_tag="ACL8LA_000880"
     CDS             23931..24917
                     /gene="jag"
                     /locus_tag="ACL8LA_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGS1812658.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24968..25591
                     /locus_tag="ACL8LA_000881"
     CDS             24968..25591
                     /locus_tag="ACL8LA_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGS1812659.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25722..27179
                     /locus_tag="ACL8LA_000882"
     CDS             25722..27179
                     /locus_tag="ACL8LA_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1812660.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27202..27483
                     /locus_tag="ACL8LA_000883"
     CDS             27202..27483
                     /locus_tag="ACL8LA_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1812661.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27560..28045
                     /locus_tag="ACL8LA_000884"
     CDS             27560..28045
                     /locus_tag="ACL8LA_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1812662.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGLYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28062..28727
                     /locus_tag="ACL8LA_000885"
     CDS             28062..28727
                     /locus_tag="ACL8LA_000885"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGS1812663.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28731..29594
                     /locus_tag="ACL8LA_000886"
     CDS             28731..29594
                     /locus_tag="ACL8LA_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGS1812664.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29596..30300
                     /locus_tag="ACL8LA_000887"
     CDS             29596..30300
                     /locus_tag="ACL8LA_000887"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGS1812665.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30479..32152
                     /locus_tag="ACL8LA_000888"
     CDS             30479..32152
                     /locus_tag="ACL8LA_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1812666.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            32264..33355
                     /gene="ulaG"
                     /locus_tag="ACL8LA_000889"
     CDS             32264..33355
                     /gene="ulaG"
                     /locus_tag="ACL8LA_000889"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGS1812667.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33469..35445
                     /gene="tkt"
                     /locus_tag="ACL8LA_000890"
     CDS             33469..35445
                     /gene="tkt"
                     /locus_tag="ACL8LA_000890"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1812668.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35562..35861
                     /gene="yajC"
                     /locus_tag="ACL8LA_000891"
     CDS             35562..35861
                     /gene="yajC"
                     /locus_tag="ACL8LA_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS1812669.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35908..36336
                     /locus_tag="ACL8LA_000892"
     CDS             35908..36336
                     /locus_tag="ACL8LA_000892"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGS1812670.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36338..36748
                     /locus_tag="ACL8LA_000893"
     CDS             36338..36748
                     /locus_tag="ACL8LA_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGS1812671.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37048..39699
                     /gene="adhE"
                     /locus_tag="ACL8LA_000894"
                     /gene_synonym="adhC"
     CDS             37048..39699
                     /gene="adhE"
                     /locus_tag="ACL8LA_000894"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGS1812672.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12161 a         7155 c         8988 g        11681 t
ORIGIN      
        1 aagggaacgg tcttgaaaac cgtcaggcgt gtaaaagcgt gcgtgggttc gaatcccaca
       61 tcctcctttt atattaacgc gggatggagc agctcggtag ctcgtcgggc tcataacccg
      121 aaggtcgtag gttcaaatcc tgctcccgca ataaggctcg gtagctcagt tggtagagca
      181 atggattgaa gctccatgtg tcggcggttc gattccgtct cgcgccattt atatattttg
      241 gaagggtagc gaagaggcta aacgcggcgg actgtaaatc cgctccttcg ggttcggggg
      301 ttcgaatccc tccccttcca ttttacgggc atagtttaaa ggtagaacta aggtctccaa
      361 aaccttcagt gtgggttcaa ttcctactgc ccgtgttaat agaattatgg cgggtgtggt
      421 gaagtggtta acacaccaga ttgtggctct ggcatgcgtg ggttcgatcc ccatcactcg
      481 cctattttat attattgggg tatagccaag cggtaaggca agggactttg actccctcat
      541 gcgttggttc gaatccagct accccagtta ctatttgccg gcgtggcgga attggcagac
      601 gcgctggact caaaatccag tgtccgcaag gacgtgccgg ttcgaccccg gccgccggta
      661 tagtatagtg ttaggaacgt tgttattctt cgttcctttt ttatattatt tttggtataa
      721 ttatagttat tcaaatttta tttagattaa gaaagtgtag gggagtatgt cttgttctat
      781 cgatttatta aaacatcggt atttgaaaaa tattaaagaa aatcctgaat tgtttgtcgg
      841 aattgagttg gagtatcctg ttgcaagttt agaaggggat gctacagatg ttgaagttat
      901 gaaggatcta tttcattatt tagtttctac tttggatctc accgtagcaa aggtagatga
      961 ttttggcaat ctgatccagt tagtagatcc gataagtcag gatgctattt tatttgaagt
     1021 ttcctataca acgattgagt ttgcatttgg taaggctgaa acgattcaag aggtcgaaaa
     1081 tcgtttcaat aattatatga atgtaattca gagaaagtta gctgaatcaa atcatgctat
     1141 tgttggctgt ggtatccatc ccaactggga taaaaatgag aattgtccag tggcttatcc
     1201 acgctatcag atgttgatgg attatttgaa tttgagtaga aatattatta aatcagattt
     1261 acatcatttc cctgaatatg gtacttttat ctgtgggagc caggttcagc tggatatttc
     1321 aaaaaccaac tacttacggg tgattaatgc ttttactcaa attgaagcgg ctaaggctta
     1381 tttatttgca aactctgaat tttcgggtgc ggattgggat acgaaaattt caagggatat
     1441 tttctgggaa gaatctatgc atggtatcta tccagagaat gttggggtca atgctagact
     1501 ccttaatgat gaagctgatt tttttgacta tctaaatcat tctgcgattt ttactgcgga
     1561 acgtgatggg cagacctatt atttttatcc tattcaggct ggggactatt tggctacgtc
     1621 cgaaatccaa gcatttgctc tgaatgggga tgaggttatt atttaccccc aagagaagga
     1681 ttttgaaact catcgtagtt accagtacca agatttaacg actcgaggaa cagttgagtt
     1741 tcgtagtgtg tgtacacagc cacttgatag gacttttgct tctgcagctt ttcacttggg
     1801 attattggtt aatttagaca agttagaagc ttacttagaa acagcacctt tctttaaagt
     1861 atttggttat gattacaagt ctttaaggag acaattttct aagaaaaatc ttacagatga
     1921 ggaagaaact acgattattg aattttccaa agacttactc ctactagctg aggagggact
     1981 agtggtgaga aataaggaag aaatgaccta tttacagcct ttgagagaag aattgagcct
     2041 ataatttctc ttataaaggg agaattttct gaaaaatcat gatataatgg acgagactat
     2101 agataaagga tagagagtaa tgacattagt ttatcaatca acgcgtgatg ccaacaatac
     2161 agtaactgcc agccaagcaa ttttgcaagg tttggcgacg gacggcggtt tgtttacacc
     2221 ggatacttat ccaaaggtag atttgaactt tgacaaattg aaagatgctt cttaccagga
     2281 agttgctaag ctagttttgt cagcattttt agatgacttt acagttgagg agttggacta
     2341 ctgtatcaac aatgcctacg atagcaaatt tgatactcca gctattgcac cattagtgaa
     2401 attagatggg caatacaatt tggaactttt ccatggttca acgattgcct ttaaggatat
     2461 ggccttgtct attttgccat actttatgac gactgctgct aagaaacatg gtttggagaa
     2521 caagattgtt atcttgacag cgacatctgg tgacacgggg aaagctgcta tggcggggtt
     2581 tgcgaatgtg cctggtactg agattatcgt cttttatcca aaggatggtg tcagcaagat
     2641 tcaagagtta caaatgacca ctcagactgg cgacaatact catgttattg ctattgatgg
     2701 taactttgac gatgcgcaaa caaatgtgaa gcacatgttt aacgacgtgg ctcttcgtga
     2761 aaaattgact accaacaagt tgcaattttc atcagctaac tctatgaaca ttggtcgtct
     2821 ggtgccacaa attgtttatt atgtttatgc ttacgctcaa ttggttaaga ctggtgaaat
     2881 tgtagctggt gaaaaggtta acttcacagt accaacagga aactttggaa atatcttggc
     2941 tgccttttat gccaaacaaa ttggtttgcc agttggtaaa ttaatctgtg cttcaaatga
     3001 caacaatgtt ttgacagact tctttaaaac acgtgtctat gacaaaaaac gtgagtttaa
     3061 ggtaacaacc agcccatcta tggatatctt ggtatcttca aacttggagc gcttgatttt
     3121 ccatcttttg ggaaataatg ctgaaaagac aactgaactt atgaatgcct tgaacacgca
     3181 aggacaatat aagttgacag actttgatgc agagattttg gacctctttg cagctgaata
     3241 tgcgactgag gaagaaacgg cagcagagat caagcgtgtt tgtgagttag attcttatat
     3301 cgaggaccct catacagctg ttgcttcagc agtttataaa aaataccaat cggccactgg
     3361 agatgtaact aagacagtga ttgcttcaac agctagtcca tacaagttcc cagtagttgc
     3421 agtagaagct gtaactggaa aagcaggttt aacagacttt gaagccttgg ctcaattaca
     3481 tgaaatctca ggcgttgcag tgccaccagc agttgatggg cttgaaatag ctccaattcg
     3541 tcacaagaca acagtggcag ctgctgacat gcaagcagcg gttgaggctt atttaggact
     3601 ttaagacaga gggagcaaac tcggttggga aaccaactga gtttcttttc atcaggagga
     3661 gagattgttt aagaaaaata aagacattct taatattgca ttgccagcta tgggtgaaaa
     3721 ctttttgcag atgctaatgg gaatggtgga cagttatttg gttgctcatt taggattgat
     3781 agctatttca ggggtttcag tagctggtaa tattatcacc atttatcagg cgattttcat
     3841 cgctctggga gctgctattt ccagtgttat ttcaaaaagc atagggcaga aagaccagtc
     3901 gaagttggcc tatcatgtga ctgaggcgtt gaagattacc ttactattaa gtttcctttt
     3961 aggatttttg tccatcttcg ctgggaaaga gatgatagga cttttgggga cggagaggga
     4021 tgtagctgag agtggtggac tgtatctatc tttggtaggc ggatcgattg ttctcttagg
     4081 tttaatgact agtctaggag ccttgattcg tgcaacgcat aatccacgtc tgcctctcta
     4141 tgttagtttt ttatccaatg ccttgaatat tcttttttca agtctagcta tttttgttct
     4201 ggatatgggg atagctggtg ttgcttgggg gacaattgtg tctcgtttgg ttggtcttgt
     4261 gattttgtgg tcacaattaa aactgcctta tgggaagcca acttttggtt tagataagga
     4321 actgttgacc ttggctttac cagcagctgg agagcgactt atgatgaggg ctggagatgt
     4381 agtgatcatt gccttggtcg tttcttttgg gacggaggca gttgctggga atgcaatcgg
     4441 agaagtcttg acccagttta actatatgcc tgcctttggc gtcgctacgg caacggtcat
     4501 gctgttggcc cgagcagttg gagaggatga ttggaaaaga gttgctagtt tgagtaaaca
     4561 aaccttttgg ctttctctgt tcctcatgtt gcccctgtcc tttagtatat atgtcttggg
     4621 tgtaccatta actcatctct atacgactga ttctctagcg gtggaggcta gtgttctagt
     4681 gacactgttt tcactacttg ggacccctat gacgacagga acagtcatct atacggcagt
     4741 ctggcaggga ttaggaaatg cacgcctccc tttttatgcg acaagtatag gaatgtggtg
     4801 tatccgcatt gggacaggat atctgatggg gattgtgctt ggttggggct tgcctggtat
     4861 ttgggcaggg tctctcttgg ataatggttt tcgctggtta tttctacgct atcgttacca
     4921 gcgctatatg agcttgaaag gataggaaat gcaaaaaaca gcttttattt gggatttaga
     4981 cgggacttta ttggactctt acgaagcgat tttatcaggg attgaggaga cttttgctca
     5041 gttttctatt ccttatgata aggagaaggt gagagagttt atcttcaagt attcggtgca
     5101 agatttgctt gtgcgggtgg cagaagatag aaatctggat gttgaggtgc taaatcaggt
     5161 gcgtgcccag agtctggctg agaagaatgc tcaggtagtt ttgatgccag gtgcgcgtga
     5221 ggtgctagct tgggcagacg aatcaggaat tcagcagttt atatatactc ataaggggaa
     5281 caacgctttt accattctca aggacttggg ggtggaatcc tattttacag agattttaac
     5341 cagtcagagt ggctttgtgc ggaagccaag tccagaagcg gctacctatc tgctagataa
     5401 gtatcagttg aattctgata atacttatta tataggggat cggactctgg atgtggaatt
     5461 tgcccagaat agtgggattc aaagcatcaa ctttttagag tctacttatg aagggaatca
     5521 caggattcaa gcgttagcag atatttcccg tatttttgag actaagtgat aaaaagattg
     5581 tgtcagtttt gtgacagaga cctaacaaac tatttcaagt aacctagttt gttacaagga
     5641 atagacagtt ctgttaaata ggcccgagag ggcttttttt ctacattttt tgtgttatga
     5701 tagacaggta ctcatttgaa aggaatttga aagaatgaag aaaagaatgt tattagcgtc
     5761 aacagtagcc ttgtcatttg ccccagtatt ggcaactcaa gcagaagaag ttctttggac
     5821 tgcacgtagt gttgagcaaa tccaaaacga tttgactaaa acggacaaca aaacaagtta
     5881 taccgtacag tatggtgata ctttgagcac cattgcagaa gccttgggtg tagatgtaac
     5941 agtgcttgcg aatctgaaca aaatcactaa tatggacttg attttcccag aaactgtttt
     6001 gacaacgact gtcaatgaag cagaagaagt aacagaagtt gaaatccaaa cacctcaagc
     6061 agactctagt gaagaagtga caactgcgac agcagatttg accactaatc aagtgaccgt
     6121 tgatgatcaa actgttcagg ttgcagacct ttctcaacca attgcagaag ttacaaagac
     6181 agtgattgct tctgaagaag tggcaccatc tacgggcact tctgtcccag aggagcaaac
     6241 gaccgaaaca actcgcccag ttgaagaagc aactcctcag gaaacgactc cagctgagaa
     6301 gcaggaaaca caagcaagcc ctcaagctgc atcagcagtg gaagtaacta caacaagttc
     6361 agaagcaaaa gaagtagcat catcaaatgg agctacagca gcagtttcta cttatcaacc
     6421 agaagagacg aaaataattt caacaactta cgaggctcca gctgcgcccg attatgctgg
     6481 acttgcagta gcaaaatctg aaaatgcagg tcttcaacca caaacagctg cctttaaaga
     6541 agaaattgct aacttgtttg gcattacatc ctttagtggt tatcgtccag gagacagtgg
     6601 agatcacgga aaaggtttgg ctatcgactt tatggtacca gaacgttcag aattagggga
     6661 taagattgcg gaatatgcta ttcaaaatat ggccagccgt ggcattagtt acatcatctg
     6721 gaaacaacgt ttctatgctc cattcgatag caaatatggg ccagctaaca cttggaaccc
     6781 aatgccagac cgtggtagtg tgacagaaaa tcactatgat cacgttcacg tttcaatgaa
     6841 tggataaacc cgacttgata acatcatttt gacgaatgag atctagcttt cgtgatggaa
     6901 agcgattctc gttcgttttt tctttgtcat actcttcgaa aatctcttca aaccacgtca
     6961 gttttatctg aaacttcaaa gctgtgcttt gagcaacctg cgactagctt cctagtttgc
     7021 tttttgattt tcattgagta tcaatttgaa tggaaaatgg aaagttatca tcttgtaatg
     7081 agttaagcaa cattcttgca atctatttta ctttatatca caattaatta gtcaaatatt
     7141 gataaatcaa taaaaagaga ggggaagaaa tgctagagat tcaagattta ctgtatcaac
     7201 tccgcttgtc tgagcaagcg agtacgcaat tgtttgaaaa aaggcttggg attagtttga
     7261 cacggtatca gattttactg tttttgctgg agcattctcc ttgtaaccaa atggcggttc
     7321 aggagcgttt gaaaattgat caggctgctt tgacacggca tttcaaaatt ttggaaacgg
     7381 aaggtttggt ggagcgtcat cgtaatcctg aaaatcagcg ggaagtgttg gtagaggctg
     7441 cgaagtatgc caaggagcag ttagtggtga atccccctct gcaacatatc agggttaagg
     7501 aagagataga aagtatctta acagagtttg agagaacaga actcagccgt ttattaaata
     7561 aattggtttt gggtattgaa aatatagaaa tttaaggaga aatagatgtc aattatttta
     7621 acaacgatcg ttgctttgga gcatttttac attttttatt tggaaagtat tgccacgcaa
     7681 tcagatgcga ctagtcgtgt atttaatatg gaaaaggaag aattggctca tccgtcagta
     7741 agttcattgt tcaaaaatca aggaatttat aaggctctgc taggagtctt tctcttgtat
     7801 gtcatttatt tctcacagaa tttagaaatt gtgactattt ttgtcttatt tgtgattggt
     7861 gctgcgactt acggctcttt aacagcggat aaaaaaatta ttttgaaaca aggtggatca
     7921 gctattttgg ccttgattag tattttactc tttaaataca cttgaaggtc gattctaatc
     7981 tcgctaatcc tttttaatcc agaataaggg aaatatgtta tacttgtttt taagaaaaaa
     8041 gtctcattga attggttttg aggagttaga aatgaaagta ttagtgacag gttttgagcc
     8101 ctttggaggg gaaaagggca atccagcttt ggaggccatt aaaggtttac cagctgaaat
     8161 ccatggtgct gaggtccgtt ggctagaggt gccgacagtt tttcacaaat ctgctcaagt
     8221 attggaagaa gagatgaatc gttatcaacc tgactttgtc ctttgtattg ggcaagctgg
     8281 tggaagaact agtttgacac ctgaacgagt gaccattaat caagacgatg catgcatttc
     8341 tgataacgaa gataatcaac cgattgaccg tcccattcgc ccagatggtg cttcggccta
     8401 ctttagtagt ttgccgatta aagcgatggt tcaagctata aaaaaagagg gcttaccggc
     8461 ctctgtttcc aatacggcag ggacttttgt ctgcagccat ttgatgtatc aggctctcta
     8521 tttggtagaa aagaaatctc catatgttaa ggcaggtttt atgcatattc cttatatgat
     8581 ggaacaggtg gtgaacagac cgactactcc agctatgagt ttagtggata ttcggcgagg
     8641 gatagaagca gcaatcggcg ctataataga acatggagat caggaactca agttggtagg
     8701 cggagaaact cattgataga aaaaagcttg aggagaaaaa ccttcaagct tttggacgtt
     8761 ttcgggccaa tactgctcgg taaaacataa ttttagtgca ttggatataa ggtaggagtg
     8821 aaaaactagc aatgccaaag gtaatccaat tgaggaagta ccaaggaaga agctgtaaat
     8881 ctaggacaaa gtgctggaac ttgtagccct tcataaagga acggctagtt tttaggattc
     8941 gtcttggtgg gacctgtcct aggtctagac tataacagag aagaaattcc acctgtgaat
     9001 aggcataata ctgtggaata tagaggatat ttcctacaat gatcaagatg agacttgcaa
     9061 gaaagtagag tccaaagacc atgaggaaac gctcggtttc aactgatgag agatctagat
     9121 ttggaaactc aggatgtagg gtgacgaatt ttttggctaa aaagctacta taaaagagga
     9181 ggtaaatccc aagtaaatta gggatactcc ataaaaagag atagaaacgt ttgagaagta
     9241 gggtcaaaaa ggtttgagaa aagcgctcct catcaaagag agctaggctg ttttttacag
     9301 atggctccgt tttagaatct ttcatgagtg tcagtgttgc atagacggaa ctggtcaaaa
     9361 gaatagtccc gataaaggag actagtagag gaaagaggta ggtttgaagt atttggccaa
     9421 gtatgctgaa aaatggctgt tctaaaacag tcccgtggat ccgagataag ggattaagaa
     9481 aaccagataa gatgaccagc atactgggaa ggatatagag gagaaagaga cggggggtgt
     9541 cagcctgaaa atgttttgac tcctgacgaa ttgtttttaa atcaattttt ggatagttca
     9601 ttctcttatt ataccatagt tcttatacat agttcgtgac agttcctact ttttttgata
     9661 aaatcataca gtgtgtcctt gggcacactg tatgaactgg gactgtcttt cccagcttcg
     9721 gaggtaaaaa atgtcagatt caccaatcaa atatcgtttg attaagaaag aaaaacacac
     9781 aggagctcgt ctgggagaaa tcatcactcc ccacggtacc tttccgacac ctatgtttat
     9841 gccagttggg acacaagcca ctgtcaaaac tcagtcacct gaagaattga aggagatggg
     9901 ttcgggaatt atcctatcaa acacctatca tctctggctt cgccctggag atgaactcat
     9961 tgcacgcgct ggtggtctcc acaagttcat gaattgggac cagcctatct tgacagatag
    10021 tggtggtttt caggtttatt ctttagcaga tagccgtaat atcacagaag aaggagtaac
    10081 ctttaaaaat catctaaatg gttctaagat gttcctatcc ccagaaaaag ccatctctat
    10141 tcagaataat ctgggttcag acatcatgat gtcctttgat gaatgtcctc agttttatca
    10201 accttatgac tacgttaaga aatcgatcga gcgtaccagc cgttgggctg agcgtggttt
    10261 gaaggctcac cgtcgtccac atgaccaagg tttgtttgga attgtgcaag gtgcaggatt
    10321 tgaagacctt cgccgccaat cagctcatga tcttgtcagc atggatttct caggctactc
    10381 tatcggtggt ttggcagtgg gagaaaccca tgaagagatg aatgcggtct tggactttac
    10441 aactcaactg ctacctgaaa ataaacctcg ttatctgatg ggtgtgggag cgccagatag
    10501 cttgatcgat ggggtcattc gtggggtgga tatgtttgac tgtgtcttac cgactcgaat
    10561 tgctcgtaac gggacttgta tgaccagtca aggacgtttg gttgtgaaaa atgcccagtt
    10621 tgctgaggac tttacgccac tggatcctga gtgtgactgc tacacatgta ataactatac
    10681 acgcgcttac cttcgtcacc tgctcaaggc tgatgaaacc tttggtatcc gcttgactag
    10741 ctaccacaat ctttacttct tgcttaacct gatgaagcaa gtgcgacaag ccatcatgga
    10801 tgacaatctc ttggaattcc gtgagtattt tgtggaaaaa tatggctata ataagtcagg
    10861 acgtaatttc taaaatggaa ttgatataaa aaaatcctaa gttttctctt aggatttttc
    10921 ttcttttttt gatagaataa agtgtacaat gaaaggaaga ataaactcgt atgcgcatta
    10981 aatggttttc cttgattagg attataggtt tacttttggt actcttgtat cacttctttc
    11041 agacgatctt tcctggagga tttttcgggg tagatgtctt tttcacattt tcaggtttcc
    11101 tgattacagc tctactcatt gaagaatttt ctaagaacaa tgagattgat ttgataggat
    11161 tttttagaag acgcttttat cggattgtgc cacctgtggt tttgatggtc ttggtgacca
    11221 tgccttttac tttcttggtt cgccaagact atgttgctgg aattggtggc cagattgcgg
    11281 gcgtcttagg ctttatgacc aacttctatg aactcctaac aggtgggagt tatgaatctc
    11341 agttcattcc tcatttgttt gttcataatt ggagtttggc agttgaggtt cactactata
    11401 ttctttgggg attggcagtt tggttcttat ccaaacaagc taaatcaaat ggtcagttga
    11461 aggggatggt ctttctctta tctgctgctg ccttcttgat cagtttcttc tccatgttta
    11521 ttggtagttt tctagtgacc tcttattcct ctgtttattt ctccagttta actcatgtct
    11581 atccattctt tttgggaagt atgttagcaa ctattgtagg tgttcgtcag acgacttccc
    11641 tcgtcaagca gttggataaa atctgggatt tacgaaagac tttggtagtt tttggaggag
    11701 gctttggttt cctagttctt ttgactttct ttgtcaaatt cacttatctt tttgcctatc
    11761 ttatcggctt cttgcttgcc agtcttgcag ctcttgccat gattctggcg gcgcgtgtct
    11821 tacatgaaaa gacacatcat atacaggagc cgaagattat cagcttttta gcggatacta
    11881 gctatgcggt ttatcttttc cattggcctt tctatatcat tttctcacag ttgacatcaa
    11941 atcttcttgc tgtattactg actctgattt gttcttatgg ctttgccagt ctgtcattct
    12001 atgtattgga accttggatt gcaggcaaga acacacctat tttacaaact cttcgtcccc
    12061 tgccttatat tcacgcaatt cttgtagcag gtacaggaat cttgaccatc attgtctgca
    12121 cggtgacctt gttggcacca caagtgggag cgtttgagac agacttgact gtcaatggct
    12181 tgaagcaagc tgcaacaaat attggccaga ccaaggtgat ggcagaacgg gcagatgcaa
    12241 acagtttggg aattgctgat ggcactatgt taattggtga ctcagtggct ttaagggcaa
    12301 atacagcact acagacagct cttcctggag cacagattaa cgcgcaggtc agcgtaacaa
    12361 ccaagaccgc aaatgaaatc atgctaaata atagccagaa taaattttta cctaagacgg
    12421 tggtcattgc gactggggta aataatcctg agaattacaa ggatgactgg gacagtatcg
    12481 tgaaaaatct tcctaaggga caccatatga ttttggtgac tccttatgag ggagataaga
    12541 caaaagagac ctatgccatc gttgagaagg ctgctgccta tatgagagaa ttggcagaga
    12601 agacacctta cattacgata gcagattgga atcaagttgc gaaagagcat ccagaaattt
    12661 gggctggaac agaccaggtt catttcggga gtgagagtag cactatcgaa gcaggagcaa
    12721 aattgtatgc agatacgatt gccacagctt tgcagacagc tcaagacaag ccggttaaat
    12781 caaaataact tgtattaaaa aagagaagag ttggagaaat ccagctcttt taaaatatct
    12841 ccaaaaaata ggtctatacc atttacaaat aaaaaagaaa ggtttataat gtaattgaca
    12901 taataaattg tagaatcaat cttttaagga ggttaacatt atgcctaact atattaaagc
    12961 ggatcagttt ttctacccac acggagttcg tcgaggtggt tacttggaac ttgtggacgg
    13021 caagtttggg aaacatgtag agcagattcc tgaaggggct gaggtgattg actatacagg
    13081 ttatagcatt gccccaggtc ttgtggatac tcatattcat ggatatgcag gtgtagatgt
    13141 gatggacaac aacattgaag gtacattgca tactatgagt gaaggacttc ttagtaccgg
    13201 tgttaccagt ttcttaccca caactttaac agccacttat gagcaattgc ttgcagtcac
    13261 tgaaaatctt ggaaaccatt ataaagaagc aacaggtgct aagattcgtg ggatttatta
    13321 tgaaggtcca tatttcacag aaacttttaa gggggcacaa aatccaactt atatgagaga
    13381 cccgggtgtt gaggagtttc attcttggca aaaagcggca aatggcttgc ttaataaaat
    13441 tgcccttgca ccagaacgtg atggggtgga agactttgtt cgtacagtta cgggcgaagg
    13501 tgtgacggtt gctcttggac attcaaacgc gacttttgat gaagccaaaa aagcagtcga
    13561 tgctggagcg agtgtttggg tgcatgccta taatggaatg cgtgggttga ctcaccgtga
    13621 attgggtatg gttggagcca tgtaccaatt gccacatacc tatgcagagt tgatctgtga
    13681 tggtcaccac gtagatccaa aggcttgcga aattcttatc aaacaaaaag gaacagaaaa
    13741 catcgctctt atcacagact gtatgacagc tgggggattg gaagacggag attatatgtt
    13801 gggagaattc ccagttgtcg ttgcaaatgg aactgcacgc ctcaaatcga caggtaactt
    13861 ggcaggttct atcctcaaac tcaaagatgg tttgaagaat gtggtcgaat ggggaattgc
    13921 gaatccgcat gaagcagtca tgatggccag cttcaaccca gctaaatccg ttcacatcga
    13981 tgacgtctgt ggccaaatcc gtgaaggcta cgacgctgac ttcatcgtat tagataaaga
    14041 tttggaattg gtagcaacct atctagatgg cgtaaaacgt tatcaagcat aagagagaag
    14101 gcagaagtta gagactagct tctgcttttt tatacagggt actttactgg taaataaaaa
    14161 gttaaaaaaa tcacaaaaaa agcttgaaga aacaaagaaa aaggcttaca attgaactat
    14221 aaatattaca aataaaaaaa cggaggagtg ctttatgaaa gcctatactt atgttaaacc
    14281 aggacttgct tcttttgttg atgtagacaa accagttatt cgcaagccaa cagacgctat
    14341 tgtgcgtatt gtaaaaacca ctatttgtgg aacagacctc catattatca aaggggatgt
    14401 tcctacttgc caaagtggta ccattcttgg ccacgaaggg attgggattg ttgaagaagt
    14461 tggggaagga gtttccaact tcaaaaaagg cgacaaggtc ttgatttctt gcgtctgtgc
    14521 ctgtggtaaa tgctactact gtaaaaaagg aatttatgcc cactgtgaag acgaaggggg
    14581 ctggattttc ggtcacttga ttgatggtat gcaggctgaa tatctacgtg tccctcatgc
    14641 agataatact ctttaccata ctccagaaga cttgtcagat gaagctttgg ttatgctgtc
    14701 agacattctg cctactggat atgaaattgg tgtcttaaaa gggaaagtag aacctggttg
    14761 cagcgtagcc attattggtt caggtccagt tggattggct gctcttttaa cagcccaatt
    14821 ctattcacca gctaaattga ttatggtaga cctagacgat aaccgcttgg aaactgccct
    14881 atcattcggt gcgactcata aggttaattc ttcagaccct gaaaaagcca ttaaagaaat
    14941 ttatgatttg acagatggtc gtggtgtgga tgtcgctatc gaagctgttg gtattcctgc
    15001 aacatttgat ttctgtcaaa agattatcgg tgtagacgga acggttgcca actgtggtgt
    15061 gcatggtaaa ccagttgaat tcgatttaga taaactttgg attcgcaaca tcaatgtaac
    15121 aactggtttg gtatctacaa atacgactcc acaattgttg aaagcacttg aaagtcataa
    15181 gattgaacca gaaaaattgg taactcacta tttcaaactc agtgaaattg aaaaagccta
    15241 tgaagtcttc agtaaggcag cagaccacca tgccattaag gtcattatcg aaaacgatat
    15301 ctcagaagcc taagtagtaa aaatattttt gtacataagt aaatagaaat tcagtcatcc
    15361 atcagatggc tggatttttt atcaaaaaat taagaaatga gcatatttct ttccttgtct
    15421 ggcggaattg gttataatat acggtacaaa ggaatgaatg aatatgtatc gtgttataga
    15481 aatgtacgga gattttgaac cgtggtggtt cttagaaggt tgggaagaag atattgtagc
    15541 aagtagaaaa tttgaccagt attatgatgc tctcaaatac tacaaaactt gctggtttag
    15601 attggaacaa gaatcccctc tttataaaag tagaagcgac ttgatgacca ttttttggga
    15661 cccggaagac caacgctggt gtgatgaatg tgatgagtat ttacaacaat accattcttt
    15721 ggctcttttg caggatgagc aggttatccc agacgaaaaa ctacgctcag gctatgaaaa
    15781 acaaaccagt caggaaagga atcgttcttg ccgtatgaaa ttaaaataga gaaaagtaac
    15841 ttttttggag ttgctttttt tatttttcta acgctttgcg aatagtatag gtgaggaggt
    15901 aagtatggtt caagaaattg cacaagaaat cattcgttca gctcggaaaa aagggacgca
    15961 ggatatttat tttgtcccta agttagacgc ctatgagctt catatgaggg taggagacga
    16021 gcgctgtaaa attggtagct atgattttga aaagtttgca gccgttatca gtcactttaa
    16081 gtttgtggcg ggtatgaatg tgggagaaaa aagacgtagt caactgggtt cctgtgatta
    16141 tgcctatgac cagaagatgg cgtctctacg tttatctact gtaggcgatt atcgggggca
    16201 tgagagtttg gttatccgtt tgttgcacga tgaggagcag gacttgcatt tttggtttca
    16261 ggatattgaa gaattaggca agcagtacag gcaacgggga ctctatcttt ttgctggtcc
    16321 ggttgggagt ggtaagacga ccttgatgca tgaattgtcc aagtcactct ttaaaggaca
    16381 gcaagttatg tccatcgaag atcctgtcga aatcaagcag gacgacatgc ttcagttgca
    16441 gttgaacgaa gcaatcggcc taacctatga aaatctaatc aaactttcct tgcgtcatcg
    16501 accagatctc ttgattatcg gagaaattcg tgacagcgag acggcgcgtg cagtggtcag
    16561 agctagtttg acaggtgcga cagtcttttc aaccattcac gccaagagta tccgaggtgt
    16621 ttatgagcgt ctgctggagt tgggtgtgag tgaagaagaa ttggcagttg ttctgcaagg
    16681 agtctgctac cagagattaa tcgggggagg aggaatcgtt gactttgcaa gcagagatta
    16741 tcaagaacac caagcagcca agtggaatga gcaaattgac cagcttctta aagatggaca
    16801 tatcacaagt cttcaggctg agacggaaaa aattagctac agctaagcaa aaaaatatca
    16861 tcaccctatt taacaatctc ttttctagcg gttttcatct ggtggagact atctcctttt
    16921 tagataggag tgctttgttg gacaagcagt gtgtgatcca gatgcgtgcg ggcttgtctc
    16981 aggggaaatc attctcagaa atgatggaaa gtttgggatg ttcaagtacc attgtcactc
    17041 agttatctct agctgaagtt catggcaatc ttcacctgag tttgggaaag atagaagaat
    17101 atctagacaa tctggccaag gtcaagaaaa agttaattga agtagcgacc tatcctttga
    17161 ttttgctggg ttttcttctc ttaattatgc tggggctacg gaattacctg ctcccacaac
    17221 tggatagtag caatattgcc acccgaatta tcggtaatct gccacaaatt tttctaggca
    17281 tggtagggct tgtttccgtg cttgcccttt tagcactaac tttttataaa agaagttcta
    17341 agatgagtgt cttttctatc ttagcacgcc ttccctttat tggaatcttt gtgcagacct
    17401 acttgacagc ctattatgca cgtgaatggg ggaatatgat ttcacaggga atggagctga
    17461 cgcagatttt tcaaatgatg caggaacaag gttcccagct ctttaaagaa atcggtcaag
    17521 atctggctca aaccctgaaa aatggccgtg aattttctca gacgatagga acctatcctt
    17581 tctttaggaa ggaattgagt ctcatcatag agtatgggga agttaagtcc aagctgggta
    17641 gtgagttgga aatctatgct gaaaaaactt gggaagcctt ttttacccga gtcaaccgca
    17701 ccatgaattt ggtgcagcca ctggttttta tctttgtggc actgattatc gttttacttt
    17761 atgcggcaat gctcatgccc atgtatcaaa atatggaggt aaatttttaa aatgaaaaaa
    17821 atgatgacat tcttgaaaaa agctaaggtt aaagctttta cattggtgga gatgttagta
    17881 gtcttgctga ttatcagcgt gcttttcttg ctctttgtac ctaatctgac caagcaaaaa
    17941 gaagcagtca atgacaaagg aaaagcagct gttgttaagg tggtggaaag ccaggcagaa
    18001 ctttatagct tggataaaaa tgaagatgct actctgagca agttacaagg agatgggcgc
    18061 atcacggaag aacaggttaa agcttataat gaatactata ctaaaaatgg aggggcaaat
    18121 cgtaaagtca atgattaagg cctttaccat gctggaaagt ctcttggttt tgggacttgt
    18181 gagtatcctt gccttgggct tatccagctc tgtccagtcc acttttgcag cagtagagga
    18241 gcagattttc tttatggagt ttgaagaact ctatcgggaa acccaaaaac gcagtgtagc
    18301 cagtcagcaa aagactagtc taaatttaga tgggcagacg cttagcaatg gcagtcaaaa
    18361 gttgacagtt cctaaaggaa ttcaggcacc atcaggccaa agtattacat ttgaccgagc
    18421 tgggggcaat tcgtccctgg ctaaggttga atttcagacc agtaaaggag cgattcgcta
    18481 tcaattatat ctaggaaatg gaaaaattaa acgcattaag gaaacaaaaa attagggcag
    18541 tgattttact ggaagcagta gtcgctctag ctatctttgc cagcattgcg accctccttt
    18601 tgggacaaat tcaaaaaaat aggcaagagg aagcaaaaat cttgcaaaag gaagaagtct
    18661 tgagggtagc taagatggcc ctgcagacgg ggcaaaatca ggtaagcatc aacggagttg
    18721 agattcaggt attttctagt gaaaaaggat tggaggtcta ccatggttca gaacagttgt
    18781 tggcaatcaa agagccataa ggtcaaggct tttaccttgt tagaatccct gcttgccctc
    18841 attgtcatca gtgggggatt actccttttt caaactatga gtcagctcct catttcagaa
    18901 gttcgctacc agcaacaaag cgagcaaaag gagtggctct tgtttgtgga ccaacttgag
    18961 gcagaattag accgttcgca gttcgaaaaa gtagaaggca atcgcctata catgaagcaa
    19021 gatggcaagg acatcgccat cggtaagtca aagtcagacg attttcgtaa aaccgatgct
    19081 agtggacggg ggtatcagcc tatggtttat ggcctcaaat cagctcagat tacagaggac
    19141 aatcaactgg ttcattttcg tttccagttt caaaaaggct tagaaaggga gttcatctat
    19201 cgtgtggaaa aagaagaaag ttaaggcagg tgttctcctc tacgcagtca ccatagcagc
    19261 catctttagt cttttgttgc aattttattt gaaccgacaa atcgcccact atcaagacta
    19321 tgctttaaat aaagaaaaat tggttgcttt tgccatggct aaacgaacca aggataaagc
    19381 cgagcaagaa agtggggaac aggtttttaa tctaggtcag gtaagctatc aaaacaagaa
    19441 aacaagcttg gtgacgactg ttcgtacgtc taagagccaa tatgagtttc tgtttccttc
    19501 agtcaaaatc aaagaagaga aaagagataa aaaggaagag gtagcgaccg attcaagcga
    19561 aaaagcggag aaaaaaaatc agaagagaag cctgaaaaga aagagaattc ctagtcaatt
    19621 caactataat gcgttgaatc cagaatagtc cactgtagtt tctagaaaat tgctggaaat
    19681 ggatgttaag ctccaattca tttgtttata tcttatttca gtttactata ctttgtgcta
    19741 aactaaagat atgaaacatg attttaacca caaagcagaa actttcgact cccctaaaaa
    19801 tatcttcctc gcaaacttgg tatgtcaagc agtcgagaaa cagattgata ttttatcaga
    19861 caaagtaatt ttagatttcg gtggtggcac gggtctatta gccttgcccc tagccaagca
    19921 ggctaagtca gttactcttg tagacatttc tgagaaaatg ttggagcaag ctcgtttgaa
    19981 agtggagcag caagcaatca agaatatcca gtttttggag caagatttac cgaaaaatcc
    20041 cttggagaaa gagtttgatt gccttgctgt tagtcgggtt cttcatcata tgcctgattt
    20101 ggatgcggct ctctcactgt ttcatcaaca tttgaaggaa gatgggaaac tcatcattgc
    20161 tgattttacc aagacagaag ctaatcatca tggatttgat ttagctgaac tggaaaacaa
    20221 gctaattgag catggttttt catctgtgca tagtcagatt ctctatagcg ctgaagacct
    20281 gtttcaagga aatcactcag aattcttttt aatagtagcc caaaaatcac tcgcctagtc
    20341 agggagtgat ttttctataa ggatggaaaa aagaagggaa atttgataag ataggaatat
    20401 ggattttgaa aaaattgaac aagcttatac ctatttacta gagaatgtcc aagttatcca
    20461 aagtgatttg gcgaccaact tttatgacgc cttggtggag caaaacagca tctatctgga
    20521 tggtgaaact gagctaaacc aggtcaaaga caacaatcag gcccttaagc gtttagcact
    20581 acgcaaagaa gaatggctca agacctacca gtttctcttg atgaaggctg ggcaaacaga
    20641 acccttgcag gccaatcacc agtttacacc agatgccatt gctttgcttt tggtgtttat
    20701 tgtggaagag ttgtttaaag aggaggaaat tactatcctc gaaatgggtt ctgggatggg
    20761 aattctaggc gctactttct tgacctcgct tgataaaaag gtggattact tgggaatgga
    20821 aatggatgat ttgctgattg atctggcagc tagcatggca gatgtaattg gtttgcaggc
    20881 tggctttgtc caaggagatg ccgttcgtcc acaaatgctc aaagaaagcg atgtggtcat
    20941 cagtgacttg cctgttggct attatcctga tgatgccgtt gcgtcgcgcc atcaagttgc
    21001 ttctagccaa gaacatactt acgcccatca cttgctcatg gaacaagggc ttaagtacct
    21061 caagtcagac ggatacgcta tttttctagc tccgagtaat ttgttgacca gtcctcaaag
    21121 tgatttgtta aaagaatggc tgaaagaaga ggcgagtctg gttgctatga ttagtctgcc
    21181 tgaaaatctc tttgctaatg ccaaacaatc taagactatt tttatcttac agaagaaaaa
    21241 tgaaatagca gtagagcctt ttgtttatcc acttgctagc ttgcaagatg caagtgtttt
    21301 aatgaaattt aaagaaaatt ttcaaaaatg gactcaaggt actgaaatat aaaatagatt
    21361 ttgttataat agttgaaaac gcttaaaaag gggtatcatg ttatgacaaa aacaattgca
    21421 atcaatgcag gaagttcaag tttgaaatgg caattatact taatgccaga agaaaaagtg
    21481 ttagccaaag gtttgattga acgtatcggt ttgaaagatt caatttcaac tgtaaaattt
    21541 gacggccgtt ctgaacaaca aattttggat attgaaaatc atacacaagc cgttaaaatt
    21601 ttattggatg acttgattcg tttcgatatt atcaaggctt atgacgagat tacaggtgtt
    21661 ggacatcgtg ttgttgctgg tggagaatat ttcaaagaat caacagttgt tgagggagat
    21721 gttttagaaa aagttgaaga gttgagtttg ttggctcctc tacacaaccc ggccaatgca
    21781 gcaggtgttc gtgccttcaa ggaattgttg ccagacatta ccagtgtagt tgtttttgat
    21841 acttccttcc acacaagtat gccagagaaa gcttatcgct accctctacc aacaaaatat
    21901 tacacagaaa acaaggttcg taaatacggt gctcatggta caagtcacca gtttgtagca
    21961 ggagaagctg caaaactctt gggacgtcca ttagaagact tgaagttaat tacctgtcat
    22021 attggtaacg gaggctcaat tacagctgtg aaagccggca aatctgtaga cacttctatg
    22081 gggttcactc ctcttggtgg tattatgatg ggaacgcgta caggggatat tgatccagct
    22141 atcattcctt atttaatgca atatacagag gattttaaca caccagaaga tatcagtcgt
    22201 gttcttaacc gtgaatcagg tcttttggga gtttctgcta attctagcga tatgcgcgat
    22261 atagaagcag ctgtagcaga agggaatcac gaggctagct tggcttatga aatgtatgtt
    22321 gaccgtatcc aaaaacatat cggtcagtac tttgcagtgc taaatggagc agatgccatt
    22381 gttttcacag caggtgtcgg tgaaaatgca gagaatttcc gtcgtgatgt aatctcaggg
    22441 atttcgtggt ttggttgtga tgttgatgat gaaaagaatg tctttggcgt tacaggagac
    22501 atctcaacag aggcagctaa aatccgtgtc ttggttattc caacagatga agaattagtc
    22561 attgcccgtg acgttgaacg cttgaaaaaa taagtgaaac taaaaaaata ttcaatacaa
    22621 ggagttggga aagttatttt tccagcttct ttttctgatg aaattgtcca aaaccttgct
    22681 atgattggct tttttgaaaa atatggtata atagtagtaa tttaatagat ggagttgagt
    22741 tttgaagaaa aactttcgtg taaaaagaga gaaagatttt aaggcgattt tcaaggaggg
    22801 gacaagtttt gctaatcgca aatttgtggt ctaccaatta gaaaaccaga aaaaccattt
    22861 tcgagtaggt ctatcagtta gcaaaaaact ggggaatgcc gtcactagaa atcaaattaa
    22921 gcgacggatt cggcatatta tccagaatgc aaaagggagt ctggtagaag atgtcgactt
    22981 tgttgtcatt gctcgaaaag gagtcgaaac cttgggatac gcagagatgg agaaaaatct
    23041 actccatgta ttaaaattat caaagattta ccgggaagga aatgggagtg aaaaagaaac
    23101 taaagttgac tagtttgcta ggactgtctc tgttaatcat gacagcctgt gcgactaatg
    23161 gggtaactag cgatattaca gccgaatcgg ctgatttttg gagtaaattg gtttacttct
    23221 ttgcgaaaat cattcgcttt ttatcgtttg atattagtat cggagtgggg attattctct
    23281 ttacggtctt gattcgtaca gtcctcttgc cagtctttca ggtgcaaatg gtggcttcta
    23341 ggaaaatgca ggaagctcag ccacgcatta aggcgcttcg agaacaatat ccaggtcgag
    23401 atatggaaag cagaaccaaa ctagagcagg aaatgcgtaa agtatttaaa gaaatgggtg
    23461 tcagacagtc agattctctt tggccgattt tgattcagat gccggttatt ttggccttgt
    23521 tccaagccct atcaagagtt gactttttaa agacaggtca tttcttatgg attaaccttg
    23581 gtagtgtgga tacaaccctt gttcttccga ttttagcagc agtattcacc tttttaagta
    23641 cttggttgtc caacaaagct ttgtctgagc gaaatggcgc tacgactgcg atgatgtatg
    23701 ggattccagt cttgattttt atctttgcag tttatgcgcc aggtggagtc gccctatact
    23761 ggacagtgtc taatgcttat caagtcttgc aaacctattt cttgaataat ccattcaaga
    23821 ttatcgcaga gcgcgaggcc gtagtacagg cacaaaaaga tttggaaaat agaaaaagaa
    23881 aagccaagaa aaaggctcag aaaacgaaat aaataaggag gaatctggta atggtagtat
    23941 ttacaggttc aactgttgaa gaagcaatcc agaaaggatt gaaagaatta gatattccaa
    24001 gaatgaaggc tcatatcaaa gtcatttcta gggagaaaaa aggctttctt ggtctatttg
    24061 gtaaaaaacc agcccaagtg gatattgaag cgattagtga aacgactgtt gtcaaagcaa
    24121 atcaacaggt agtaaaaggc gttccgaaaa aaatcaatga tttgaacgag cctgtgaaga
    24181 cggttagtga agaaaccgtt gaccttggtc atgtggttga tgctattaaa aaaatagagg
    24241 aagaaggtca aggtatttct gatgaagtca aggctgaaat cttaaaacat gaaagacatg
    24301 ccagcactat cttagaagaa actggtcaca ttgagatttt aaatgaactt caaatcgagg
    24361 aagcgatgag ggaagaagca ggcgctgatg accttgaaac tgagcaagat caaactgaaa
    24421 atcaagactt gaaagagatg ggcttgaagg tcgagcaaag ttatgatatt gcccaggtgg
    24481 ctacggatgt gactgcctat gttcaagcga ttgtggatga catggatgtt gaagctacac
    24541 tttcaaatga ttataaccgt cgtagcatca atctacaaat tgacaccaac gaaccaggtc
    24601 gtattatcgg ctaccatggt aaagtcttga aggccttgca actgttggct caaaattatc
    24661 tttacaaccg ctattccaaa accttctacg ttacaatcaa tgtcaatgat tatgtcgaac
    24721 accgtgcaga agtcttgcag acctatgcgc aaaaattggc gattcgtgtt ttagaagaag
    24781 gacgtagtca tcaaacagat ccaatgtcga atagtgaacg caagattatc caccgtatta
    24841 tttcacgtat ggatggcgtg accagttact ctgaaggtga tgagccaaat cgctatgtcg
    24901 ttgtagatac agaataagta aaatcagggt tgacctgatt ttttgctagc tagaggaggt
    24961 taaactgatg ttgaataaga taagagacta tttagacttt gctggtttgc agtaccgtaa
    25021 tcctgataaa gcgggagcag agcgagagaa gatgctggca ttccgccaca aaggacaaga
    25081 ggcccgaaag gcttttacag aactggccaa agcctttcaa gcaagccatc cagaatggca
    25141 actccaacag actagtcagt ggatgaatca ggcccagcgt ttgagaccac atttttgggt
    25201 ttatctacag agagacggac aagtgacaga acctatgatg gccttacgtt tgtatgggac
    25261 atctactgac tttggaattt ctttggaagt cagtttcatc gaacgtaaga aggatgagca
    25321 aacactgggc aagcaggcca aagttttaga cattccaacc gttaaaggga tttattatct
    25381 aacctactct aatggtcaaa gtcaacggtg ggaggcgaat gaagaaaagc gtcgtacttt
    25441 acgcgagaag gtgagaagtc aagaagttcg aaaagtttta gtgaaggtag atgttcctat
    25501 gacagaaaat tcgtctgaag aagaaatcgt agaaggctta ttgaagtctt attctaaaat
    25561 tcttccctat tatctagcta cgagaaaata agataatttg taaaacatca taaatcatac
    25621 agtccaagag tgaacagtcc gctgtgtaat tcttggtctt tttgtttgcg ctttcgcatt
    25681 atataataaa cttacaaaaa caattcaaaa ggagaacaat tatggaagtc gtttcaagtg
    25741 ttctaaattg gttttctagc aatattttgc agaatcccgc atttttcgta ggtttattgg
    25801 tgttgatagg atatgcactt ttgaaaaaac ctgcccatga cgttttttca gggtttgtta
    25861 aagcaacagt agggtatatg ttgcttaacg tgggtgctgg tggtttggtt acaacctttc
    25921 gtccaatctt agcagctctt aactacaaat tccaaattgg tgcagcggtt atcgaccctt
    25981 actttggact tgctgcagca aacaacaaaa ttgcagcaga gtttccagat tttgttggaa
    26041 ctgcaactac agctctattg attggttttg gaataaatat cttgctcgta gctcttcgaa
    26101 agattacgaa ggtaagaacc ctctttatta ctggtcacat catggtacaa caagctgcaa
    26161 cagtatctct tatggttcta ttcttagtac cacaattgcg caatgcttac ggtacagcag
    26221 cgattggtat catctgtgga ctttactggg cagttagttc aaatatgact gttgaggcaa
    26281 ctcaacgctt gactggtggt ggcggatttg cgattggtca ccaacagcaa tttgcaatct
    26341 ggtttgtaga taaagtagca ggacgctttg gtaagaaaga agaaagttta gacaatctta
    26401 aattacctaa gttcctctca atcttccacg atacagttgt tgcatctgct accttgatgc
    26461 tcgtattctt cggggccatt cttttaatct tgggtccaga cattatgtct aataaagaag
    26521 tcatcacttc aggaactcta ttcaatcctg ctaaacaaga tttctttatg tacattatcc
    26581 aaacagcctt taccttctca gtttacttgt tcgttttgat gcaaggtgtc cgaatgttcg
    26641 tatctgagtt gacaaacgcc ttccaaggta tttcaaacaa attgttgcca ggttcattcc
    26701 cagcggttga cgttgcagct tcttatggat ttggttctcc aaatgctgtc ttgtcaggat
    26761 ttacctttgg tttgattggt caattgatta caattgtctt gctcatcgtc tttaaaaatc
    26821 cgattcttat tattacagga tttgtaccag tgttctttga caatgcagcc attgcggtct
    26881 acgctgataa acgcggcgga tggaaagcgg ctgttatcct ttcctttata tcaggtgtcc
    26941 ttcaagttgc tctaggagct ctttgtgtgg cccttctcga tttggcatct tatggtggct
    27001 accatggaaa tatcgacttt gaattcccat ggcttggatt tggatatatc ttcaaatacc
    27061 ttggtattgt tggttatgta cttgtgtgtc tcttcttgct tgttattcct caacttcaat
    27121 ttgccaaagc aaaagataaa gagaaatatt acaacggtga agttcaagaa gaagcttagt
    27181 atctagaaaa ggagaaataa aatggttaaa gtattagcag cgtgcggaaa tggaatgggt
    27241 tcatcaatgg ttatcaagat gaaggttgaa aatgctctcc gtaagcttaa tcaaacagat
    27301 tttacagtca attcatgcag tgtcggtgaa gctaaaggtt tagcagtagg atatgacatc
    27361 gtaatcgctt ctcttcattt gattcaagaa ttggaagggc gaactaatgg gaagttaatt
    27421 ggacttgata acttgatgga tgataaagaa atcaccgaaa aactcagtca agcactacag
    27481 taaaaggttg gagggggctg gacagaaact gagagttatc gtttctgtcc ttctccctct
    27541 ttaaataaag gaggcagata tgaatttaaa acaagcttta attgacaatg actcgatccg
    27601 actaggttta gaggctaaca attggaaaga agcagtcaag gtagcagtag atcccttaat
    27661 tgaaagtggg gcaattttgc cagagtatta cgatgctatc attgaatcga ctgaagagta
    27721 tgggctttac tatatcttga tgccaggtat ggctatgccc cacgctagac ctgaagctgg
    27781 tgtgcaaagt gatgcctttt cattgattac cttacaaaat cctgttgtat tttcagatgg
    27841 gaaagaggta tctgttttgt tggcactagc agcaacaagc tcaaaaattc acacaagtgt
    27901 agccattcca caaattattg ccctgtttga attagaagat tctattgcac gtttacaggc
    27961 ttgccagact aaagaagatg tcttggctat gattgaagaa tctaaggata gcccttatct
    28021 cgaaggattg gatttggaaa gttagaaaga ggaataaaga aatgacaaaa agaataccta
    28081 atttacaagt tgcattagac cattcagact tgcaaggagc gattaaagca gctgtttctg
    28141 ttggtcagga agtagatatt atcgaagctg gaactgtttg cttgcttcaa gttggaagtg
    28201 aactggctga agtcttgcgt agccttttcc cagataagat tattgtggca gacacaaaat
    28261 gtgctgatgc tggtggaaca gttgctaaaa ataatgcggt tcgtggagca gactggatga
    28321 cttgtatctg ttgtgcaacc atccctacta tggaagcagc tctaaaggct atcaagactg
    28381 aacgaggaga acgaggcgaa atccagatcg agctttatgg cgattggact tttgaacaag
    28441 ctcagctttg gctagatgca ggtatctcac aagctattta tcaccaatct cgtgatgctc
    28501 ttcttgctgg tgaaacttgg ggtgaaaaag accttaataa ggttaaaaaa ctcattgaca
    28561 tgggcttccg tgtatctgta acaggtggtc tagatgtaga tactctcaaa ctctttgaag
    28621 gtgttgatgt ctttaccttt atcgcaggtc gtggaattac agaggctgcg gatccagcag
    28681 gagcagcgcg tgccttcaag gatgaaatca aacgaatttg ggggtaaatc atggtacgtc
    28741 caattggaat ttatgaaaag gcaaccccaa cacactttac ttggctagaa cgtttaaatt
    28801 ttgccaagga gttaggcttt gattttgtcg agatgtctat tgacgaacgt gacgagcgtt
    28861 tagcaagact tgactggagt aaggaagaac gcttggaagt tgtcaaagca atctatgaaa
    28921 ctggtgttcg tattccttct atctgttttt caggccatcg tcgctaccca ttgggttcaa
    28981 aagatccagt tctagaggaa aaatctctag aactcatgaa aaaatgtatc gaattagctc
    29041 aagacttggg agttcgtacg attcaattag ctggttacga tgtttactat gaggaaaagt
    29101 caccccagac acgccaacgt tttatcaaaa atttgagaaa agcctgtgac tgggctgaag
    29161 aagctcaggt ggtacttgct attgaaatta tggatgatcc tttcatcaat agcatcgaaa
    29221 aatatttggc tatagaaaaa gagattgact ctcccttcct ctttgtatat ccagatattg
    29281 gtaatgtgtc tgcatggcat aatgatatct atagtgagtt ttatcttggt catcatgcca
    29341 tcgcagctct ccatctcaag gatacttatg cagtgacaga aagttcaaag ggccagttcc
    29401 gagatgtacc tttcgggcaa ggttgtgtca aatgggaaga agctttcgat attttaaagg
    29461 aaaccaatta taatggacct ttcctaatcg aaatgtggtc tgaaaattgt gaaacagtag
    29521 aagaaacacg cgcagccatt caagaggcgc aagcttttct ctatccactc attaagaaag
    29581 caggtttgat gtaagatgaa tcaagtaatc aatgctatgc gtaaacgagt ctgtgatgcc
    29641 aatcaatcat tgccaaaaca tggacttgtc aaatttacct gggggaatgt atctgaagtt
    29701 aatcgcgaac tcggtgtcat tgttatcaaa ccatcaggcg tggattatga cgaattgaca
    29761 cctgaaaaca tggtagtgac tgatctagat ggtaagatcc tagaagggga tttaagacca
    29821 tcttccgacc tcccaactca tgtgcaatta tataaggctt ggtcagaaat tggtagtgtg
    29881 gttcacaccc attcgacaga agctgttggt tgggctcagg caggtcgtga tattcctttc
    29941 tacggaacaa cccatgcaga ttatttctac ggttcaatcc cttgcgcccg tagtttgacc
    30001 aaggacgaag tagaagtggc ctatgaaaaa gatactggcc tggttatcgt agaagagttt
    30061 gaacatcgcg gacttaaccc ggttgaagta ccaggaattg ttgtacgcaa tcacggtcca
    30121 ttcacctggg gcaaaaatcc agagaatgct gtttatcact ctgtcgtact agaggaagta
    30181 tcaaagatga atcgctttac agaacaaatc aatccaagag ttggacctgc tccccagtac
    30241 atactagaaa aacactacca acgtaaacat ggaccaaatg cttattatgg tcaaaagtaa
    30301 gaacgatgaa ggaggagaaa aagataaatt tagctcctct ttttacattt gatttttatt
    30361 gagagtaaag ttggaattga agtaatttta aaagattttt tagaaatagc gcttgatata
    30421 tatatggtaa aataaaaaga attgctgtga tatcaataga tttgggggat tttttaatat
    30481 ggtactggat aaggcaagtt gtgatttgct tcaatatttg atggatcaag aaacgtccaa
    30541 aacgattatg gcgatttcga aagatttgaa agagtcaaga aggaaaattt attatcacat
    30601 tgacaaaatc aatgctgctc tggttgacga ggcgcttcac atcattagta ttccacgaat
    30661 tggtattcac ttaacggaag agcagagaga tgcttgttgt aaactattat cggaagtaga
    30721 ttcgtacgat tatatcatga gtgcgcatga acgtatgatg ataatgttac tatggatagg
    30781 tatttctaaa gaacgtatta cgattgaaaa attgatagag ttaacagagg tatctaggaa
    30841 tactgttctc aatgatttga atagtattcg ttatcaacta actttggaac aatatcaggt
    30901 gatcttgcaa gtgagcaagt cacagggata ccaccttcat gcccaccctc ttaataaaat
    30961 tcagtatctt caatcgcttc tatatcatat ttttatggaa gaaaatgcca cttttgtatc
    31021 tattttagaa gataagatga aagagaggtt agatgatgag tgtttgcttt ctgttgaaat
    31081 gaaccaattt tttaaggaac aggttccttt agttgaacaa gatttaggga agaaaataaa
    31141 ccatcatgaa ataactttta tgttgcaggt tctaccttat ttgctgttaa gctgtcataa
    31201 tgttgaacag tatcaagaaa gacatcagga tatagagaaa gaattttctt tgataagaaa
    31261 aagaatagag tatcaggtgt ctaagaaatt aggagaacgg ttgtttcaaa agtttgaaat
    31321 ttctttgtca ggacttgaag tttctcttgt agctgttctc ctcctctcct atcgtaagga
    31381 tttggatatt catgcagaaa gtgatgattt tcggcaatta aaacttgctt tagaagaatt
    31441 tatctggtat tttgaatcac aaatccgaat ggagattgag aacaaggatg atttgttacg
    31501 aaatttgatg atccactgta aagccttgtt atttagaaag acttacggta ttttttctaa
    31561 aaatcctcta acaaaacaaa ttcgatccaa gtatggagaa ttatttttag tcactagaaa
    31621 atctgcggaa attttagaag gagcatggtt tattcggcta acagacgatg atattgccta
    31681 tttgacgatt catattggag gatttttaaa gtatacacca tcgtctcaaa aaaatatgaa
    31741 aaaagtttat ctcgtttgtg atgaaggtgt tgcggtttcg agacttttgc tgaaacaatg
    31801 caaactttat tttccaaatg agcaaattga cactgtattt acaacagaac aatttaagag
    31861 tgtggaagat attgcacaag ttgatgtagt gattactact aatgatgatt tggatagcag
    31921 atttccgatt ttaagggtta atcctatcct tgaagcagaa gatattttga aaatgctaga
    31981 ctatcttaaa cacaatatat ttcgtaataa gagcaaaagt ttcagtgaaa atctttctag
    32041 tcttatttcg tcttatattg tagacagcaa gttggctagt aagttccaag aagaggttca
    32101 aacacttata aataaagaaa tagtagttca agcttttttg gaagatattt gaaggacagt
    32161 ccaatgatga acacaaacct gtgtttttct tggtcttttt tagtgttttg aagggtggta
    32221 tactaatctc aaagataaca attatatcca aaggaggcaa catatgccaa acgtcaaaga
    32281 aattacaaga gagtcatgga ttttagccac tttcccagag tggggaacat ggttgaacga
    32341 agaaatcgaa gaagaagtcg tacctgaagg caactttgcc atgtggtggc taggcaactg
    32401 tggtacttgg attaagacac cagctggtgc taacgttgtc atggaccttt ggtcaaaccg
    32461 tggaaaatca accaaaaaag tgaaagatat ggttcgtggg caccaaatgg caaatatggc
    32521 aggtgttcgt aagttgcaac caaacttgcg tgttcagcca atggttatcg atccatttgc
    32581 tatcaacgaa ctagactatt acttagtttc acacttccac agtgatcata tcgacccata
    32641 cacagctgca gcaattctca acaatcctaa gttagagcat gttaagttta tcggtcctta
    32701 ccactgtgga cgaatctggg aaggatgggg tgttccaaaa gaacgtatca tcgttgttaa
    32761 accaggtgac actatcgaat taaaagatat gaagattcat gcagtagaat catttgaccg
    32821 tacttgcttg gtaactctcc cagtgaacgg tgctgatgag acaggcggtg aacttgctgg
    32881 cttggctgtt acagatgaag aaatggctca aaaggctgtt aactatatct ttgaaacacc
    32941 aggtggaacc atctatcatg gtgcagattc tcacttctca aactattttg caaaacatgg
    33001 taaagacttt aaaattgatg ttgctttgaa taactatggt gaaaatccgg taggtatcca
    33061 agacaaaatg acatctatcg atcttcttcg tatggcagaa aatctgcgta ccaaagtcat
    33121 tatcccagtt cactatgata tctggtctaa cttcatggct tctactaatg agattctaga
    33181 actttggaaa atgcgaaaag atcgcttgca atacgatttc catccattta tctgggaagt
    33241 tggcggtaag tacacttatc ctcaagatca acacttagta gaataccatc atccacgtgg
    33301 ttttgatgat tgttttgaac aagactctaa cattcaattt aaagctttgc tataatataa
    33361 aaatattctt tggaggttat ccgtatgttg catgatacgg ataattttca tataatagta
    33421 aaatagaatg tgtgattcaa taatcacctc aaatagaaag gaaattctat gtcaaatcta
    33481 tctgttaatg caattcgttt tctaggtatt gacgccatta ataaagccaa ctcaggtcat
    33541 ccaggtgtgg ttatgggagc ggctccgatg gcttacagcc tctttacaaa acaacttcat
    33601 atcaatccag ctcaaccaaa ttggattaac cgcgaccgct ttattctttc agcaggtcat
    33661 ggttcaatgc tcctttatgc tcttcttcac ctttctggtt ttgaagatgt cagcatggat
    33721 gagattaaga gtttccgtca atggggttca aaaacaccag gtcacccaga atttggtcat
    33781 acggcaggga ttgatgctac gacaggtcct ctagggcaag ggatttcaac tgctactggt
    33841 tttgcccaag cagaacgttt cttggcagcc aaatataacc gtgaaggcta caatatcttt
    33901 gaccactata cttacgttat ctgtggagac ggagacttga tggaaggtgt ctcaagcgag
    33961 gcagcttcat acgcaggttt gcaaaaactt gataagttgg ttgttcttta tgattcaaat
    34021 gatatcaact tggatggtga gacaaaggat tcctttacag aaagtgttcg tgaccgttac
    34081 aatgcctacg gttggcatac tgccttggtt gaaaatggaa cagacttgga agccatccat
    34141 gctgctatcg aaacagcaaa agcttcaggc aagccatctt taattgaagt gaagacggtt
    34201 attggatatg gttctccaaa caaacaagga actaatgctg tacacggcgc ccctcttgga
    34261 gcagatgaaa ccgcatcaac tcgccaagcc ctcggttggg attacgaacc atttgaaatt
    34321 ccagaacaag tatatgctga tttcaaagaa catgttgcag accgtggcgc atcagcttat
    34381 caagcttgga ctaaattagt tgcagattat aaagaagctc atccagaact ggctgcagaa
    34441 gtagaagcca tcatcgacgg acgtgatcca gtcgaagtga ctccagcaga cttcccagct
    34501 ttagaaaatg gtttttctca agcaactcgt aattcaagtc aagatgcctt gaatgttgtg
    34561 gcagctaaat taccaacctt cctaggtgga tcagctgacc ttgctcactc aaacatgact
    34621 tatatcaaga cggatggact tcaagacgat accaatcgct tgaatcgtaa cattcagttt
    34681 ggtgttcgtg aatttgcaat gggaacaatc ttgaacggga tggcccttca tggtggactt
    34741 cgtgtatacg gtggaacttt cttcgtcttc tctgactatg tgaaggcagc tgtccgcttg
    34801 tcagccttac aaggacttcc tgtgacttat gtctttaccc atgattcaat cgcagttggg
    34861 gaagatggtc cgactcacga accagttgag catttagcag gtcttcgtgc tatgccaaat
    34921 ctaaatgttt tccgtccagc agatgcgcgt gaaacgcaag cagcttggta ccttgcagtg
    34981 acaagtgaga aaacaccaac tgcccttgtc ttgacacgtc aaaacttgac tgttgaagat
    35041 ggaacagact tcgacaaggt tgctaaaggt gcttatgttg tatatgaaaa tgcagccgac
    35101 tttgatacca tcttgattgc gacaggttca gaggttaatc ttgctgtctc agctgctaaa
    35161 gaattggcta gtcaaggcga aaaaatccgc gtagtcagca tgccatctac agatgtcttt
    35221 gataaacaag atgcagctta caaggaagag attcttccaa atgcagtccg ccgtcgtgtt
    35281 gcagtcgaaa tgggtgcaag tcaaaactgg tacaaatatg ttggtctcga tggtgccgtt
    35341 ctaggtattg ataccttcgg agcctctgcc ccagcaccaa aagtattggc agaatatggc
    35401 tttactgtag aaaatcttgt aaaaattgtt cgaaacttga aataatccta aaaatcaggg
    35461 cgtaagctct ggtttttctt accagaaaag taaggtacaa tcttgtaaaa gtagctgaaa
    35521 tttgatatag tagtcctatg taaaagacaa aggagaaaac aatgaatcca aatattactt
    35581 ttttaatcat gcttgtaggt atgatggcct tgatgttctt tatgcaacgt tctcaaaaga
    35641 aacaagctca aaagcgtatg gaaagcttaa ataaactaca aaaaggctat gaagtgatta
    35701 caatcggtgg actttatgga acagtcgatg aagtagatac ggagaaggga acaatagttc
    35761 ttgacgtaga tggagtttac ttgacttttg aactagctgc tatcaagaca gtattaccgc
    35821 tgaaagaaac agcttcacta gaaggcgcaa ttgaaaaata agacgggatc acgaactccc
    35881 gtttttctat aaaagaaagg aaatgggatg aaaaaattag tctttgtctg tctgggaaat
    35941 atttgccgta gccctatggc cgagtttgtt atgaaatcaa tgacagataa ctacgaaatc
    36001 caaagtcgag caacttcctc ttgggaacat ggcaatccga ttcataaggg gactcaggga
    36061 atttttcaag agtatgagat tccttatgac aagaacaaga catcgcttca gattagtaag
    36121 gaagattttg aagcctttga ttatattatc ggaatggacg cttcaaatat ttctgactta
    36181 tgtcagatgt gtccagtaga ctgtcaagat aagatttact cattttcatc tgaaagtgtt
    36241 ccagaccctt ggtatacagg agattttgaa gaaacctatc gacgtgttca agagggctgt
    36301 caagcttggt tagaacgttt agaaaaggag agttaaggtg gaaaatctta cgaattttta
    36361 cgaaaagtat cgtgtctatc tgactcgtcc acgtttagag cttttggcag tagttaccat
    36421 tgttttttgt gctgtactcg tcttttttct aaatattcca ggaaaaggtg tcttaaaact
    36481 cgataatgga acgattgttt atgatggcag tcttgtccgt ggtaaaatga atggccaagg
    36541 taccattacc ttccaaaatg gagaccaata tacaggtggc ttcaacaatg gagccttcaa
    36601 cggaaaaggt acctttcaat ctaaagaagg ctggacctac gaaggtgatt ttgtaaatgg
    36661 tcaggctgaa ggaaaaggga aactaacaac agaacaagaa gtcgtttatg aaggaacttt
    36721 taaacaaggc gtttttcaac aaaaataaag cctccttatc aaaggaggta ttattagaat
    36781 cacaaggtaa gcgcttacct gtaaatccct ttctttccaa atccctcttc caagcaagtt
    36841 tgtgaaataa aaaatatttg aaataaattt cacaaacttc aaagataaaa cttgataaga
    36901 aaagaaaatg agaaaagttt cacaagagtt taaaaattct ttgtgaaatg tttatgaaac
    36961 tgtttacaaa gttgaaataa tgcgttataa taaacttgtg aaaaaattaa caaaggatat
    37021 cgttccttga aagctatgga ggaaaatatg gctgataaaa aaactgtgac accagaggaa
    37081 aagaaactcg ttgctgaaaa acacgtagat gagttggttc aaaaagctct agttgccctt
    37141 gaagaaatgc gtaaattgga tcaagaacaa gttgactaca tcgttgccaa agcatcagta
    37201 gcagctttgg atgcccacgg agaattggct ttacatgcct ttgaagaaac aggacgtggt
    37261 gtatttgaag acaaagcaac taagaacttg tttgcttgtg aacacgtagt aaacaatatg
    37321 cgccacacta agacagttgg cgttatcgaa gaagacgatg taacaggatt gactcttatt
    37381 gctgaaccag ttggtgttgt ttgtggtatt actccaacaa caaacccaac atcaacagca
    37441 atcttcaaat cattgatttc attgaagaca cgtaacccaa tcgtctttgc cttccatcca
    37501 tcagcacaag aatcatctgc tcatgcagct cgtatcgtcc gcgatgcagc tatcgcagct
    37561 ggtgctcctg aaaactgtgt gcaatggatt actcaaccat ctatggaagc aacaagtgcc
    37621 cttatgaacc acgaaggtgt tgcgacaatc cttgcaacag gtggtaatgc catggttaag
    37681 gcggcttatt catgtggtaa accagctctt ggggtaggtg ccggaaacgt tccagcttat
    37741 gttgaaaaat cagcaaacat tcgtcaagca gcacacgata tcgtcatgtc taaatcattt
    37801 gataacggta tggtctgtgc atctgaacaa gcagttatca ttgataaaga aatttacgat
    37861 gaatttgtag cagagttcaa atcttaccac acttactttg taaacaaaaa agaaaaagct
    37921 cttcttgaag agttctgctt cggcgtcaaa gcaaacagca aaaactgtgc tggtgcaaaa
    37981 ttgaacgctg acatcgttgg taaaccagca acttggattg cagaacaagc aggatttaca
    38041 gttccagaag gaacaaacat tcttgctgca gaatgtaaag aagttggcga aaatgagcca
    38101 ttgactcgtg aaaaattgtc accagttatt gcagttttga aatctgaaag ccgtgaagat
    38161 ggtattacta aggctcgtca aatggttgaa tttaacggtc ttggacactc agcagctatc
    38221 cacacagctg acgaagaatt gactaaagaa tttggtaaag ctgttaaagc tattcgtgtt
    38281 atctgtaact caccttctac ttttggtggt atcggggacg tttacaatgc cttcttgcca
    38341 tcattgacac ttggatgtgg ttcttacgga cgcaactcag ttggggataa cgttagtgcc
    38401 attaacctct tgaatatcaa aaaagtcgga agacggagaa ataacatgca atggatgaaa
    38461 cttccttcaa aaacatactt tgaacgtgat tcaattcaat accttcaaaa atgtcgtgac
    38521 gttgaacgtg tcatgatcgt tactgaccat gccatggtag agcttggttt ccttgatcgt
    38581 atcatcgaac aactggacct tcgtcgcaat aaggttgttt accaaatctt tgcggatgta
    38641 gaaccggatc cagatatcac aactgtaaac cgtggtactg agattatgcg tgccttcaaa
    38701 ccagatacca tcatcgcact cggtggtggg tctccaatgg atgctgccaa agtaatgtgg
    38761 ctcttctacg agcaaccaga agtggacttc cgtgaccttg tccaaaaatt catggatatc
    38821 cgtaaacgtg ccttcaagtt cccattgctt ggtaagaaga ctaaattcat cgcgattcca
    38881 actacatctg gtacaggatc tgaagtaaca ccatttgccg ttatctctga taaagcaaac
    38941 aaccgtaaat acccaatcgc tgactactca ttgacaccaa ctgtggcaat cgtagatcct
    39001 gctttggtat tgacagttcc aggatttgtt gctgctgata ctggtatgga cgtattgact
    39061 cacgcgacag aagcatacgt atcacaaatg gctagtgact acactgatgg tttagcactt
    39121 caagccatta aattggtctt tgaaaatctc gaaagctcag ttaagaatgc agacttccac
    39181 tcacgtgaga aaatgcataa cgcttcaaca atcgctggta tggcctttgc caatgccttc
    39241 ctaggtattt ctcactcaat ggcccataag attggtgcgc aattccacac aatccacggt
    39301 cgtacaaatg ctatcttgct tccatacgtt atccgttaca acggtacacg tccagctaag
    39361 acagcaacat ggcctaagta caactactac cgtgcagatg aaaaatacca agatatcgca
    39421 cgcatgcttg gacttccagc ttctactcca gaagaagggg ttgaatctta cgcaaaagct
    39481 gtctacgaac tcggtgaacg tattgggatc caaatgaatt ttagagacca aggaattgac
    39541 gaaaaagaat ggaaagaaca ttctcgtgaa ttagccttcc tggcttatga agaccaatgt
    39601 tcaccagcta acccacgtct tccaatggta gaccatatgc aagaaatcat cgaagatgca
    39661 tactatggct acaaagaaag accaggacgc cgtaaataat tgtttatcag tctagaagca
    39721 agacaaaaac tcaatttgag ggaaagatcc agtaattttt ctatgataaa aggcatccta
    39781 tcaaggtttt tgaacacctg ataggatgcc tttttatgat attgaggcct ttttgccctt
    39841 tttgaaaaac tagaatagaa acaaaatata taatagattg aaactggaat agtacacttc
    39901 tgcttctaaa acattgttag aattcgattt gactgtcctg atcgatttgt cctattccta
    39961 tttcatttta ttatataaaa aatat
//
LOCUS       DBERYS010000015        39655 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.015, whole genome shotgun sequence.
ACCESSION   DBERYS010000015 DBERYS010000000
VERSION     DBERYS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.015"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..895)
                     /locus_tag="ACL8LA_000895"
     CDS             complement(<1..895)
                     /locus_tag="ACL8LA_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HGS1812673.1"
                     /translation="MILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLEKKYEE
                     AKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEEKAKKAEEAAKEVDDASLAVQKAY
                     VEQRKAQEEKNKHSGNPEYAKKLAEADKKITEETTKLTDAQTKFQSVQAMVVPEPEQL
                     AETKKKAEEAKAEEKVAKRKYDYATLKVVLAKKEVEAKELEIEKLQYEISTLEQEVAT
                     AQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLLDSLDP
                     EGKTQDELDKEAAEAELDKKVE"
     gene            complement(1195..1734)
                     /locus_tag="ACL8LA_000896"
     CDS             complement(1195..1734)
                     /locus_tag="ACL8LA_000896"
                     /inference="COORDINATES: protein motif:HMM:NF037419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HGS1812674.1"
                     /translation="MLFYFEKIWSLSLKLLDCILDYQERFNRKACQVSTNYKYLETFK
                     VNFCLTDLHHLFGLHKITRDYASQTKPAIQAGVFILEDFRNILLYNDVIERISLYEFI
                     GDIFYFKITSCCIVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFT
                     SAKKYAKVRKTDVKEMKWL"
     gene            complement(2157..2354)
                     /locus_tag="ACL8LA_000897"
     CDS             complement(2157..2354)
                     /locus_tag="ACL8LA_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812675.1"
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
     gene            complement(2833..3561)
                     /locus_tag="ACL8LA_000898"
     CDS             complement(2833..3561)
                     /locus_tag="ACL8LA_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812676.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            complement(3558..3890)
                     /locus_tag="ACL8LA_000899"
     CDS             complement(3558..3890)
                     /locus_tag="ACL8LA_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS1812677.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(3946..4848)
                     /locus_tag="ACL8LA_000900"
     CDS             complement(3946..4848)
                     /locus_tag="ACL8LA_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812678.1"
                     /translation="MMRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(4854..5171)
                     /locus_tag="ACL8LA_000901"
     CDS             complement(4854..5171)
                     /locus_tag="ACL8LA_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812679.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGDWNTFKNNIKGLARASSGFRF
                     AARETGLWAAIWSTRVYSYTYVVSAFAPILGSVAAVNAALAVIGGISVAGITYALWTR
                     KFY"
     gene            complement(5316..6668)
                     /locus_tag="ACL8LA_000902"
     CDS             complement(5316..6668)
                     /locus_tag="ACL8LA_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGS1812680.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADNKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLLPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(6665..7132)
                     /locus_tag="ACL8LA_000903"
     CDS             complement(6665..7132)
                     /locus_tag="ACL8LA_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1812681.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(7125..9344)
                     /locus_tag="ACL8LA_000904"
     CDS             complement(7125..9344)
                     /locus_tag="ACL8LA_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGS1812682.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIMEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            9810..10673
                     /locus_tag="ACL8LA_000905"
     CDS             9810..10673
                     /locus_tag="ACL8LA_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1812683.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(10962..12353)
                     /gene="argH"
                     /locus_tag="ACL8LA_000906"
     CDS             complement(10962..12353)
                     /gene="argH"
                     /locus_tag="ACL8LA_000906"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS1812684.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDVIGSLAH
                     VQMLGQTGILSLEESEKIQVGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGYVLDKLAHLKGVLLDLAENHVATIMPGYTHL
                     QHAQPISFAYHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKQPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(12382..13579)
                     /locus_tag="ACL8LA_000907"
                     /pseudo
     CDS             complement(12382..13579)
                     /locus_tag="ACL8LA_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(13596..14402)
                     /locus_tag="ACL8LA_000908"
     CDS             complement(13596..14402)
                     /locus_tag="ACL8LA_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812685.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLVTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYSVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(14609..15250)
                     /locus_tag="ACL8LA_000909"
     CDS             complement(14609..15250)
                     /locus_tag="ACL8LA_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812686.1"
                     /translation="MIELENITKTIGGKVILDRLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(15247..17355)
                     /locus_tag="ACL8LA_000910"
     CDS             complement(15247..17355)
                     /locus_tag="ACL8LA_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS1812687.1"
                     /translation="MKKISHLCMLLLLLCTTFFVLNVNVTREIVRIQEMGKTTYSFDL
                     YLKDVTKPTETILQFFEEVASQDKVSIIKVDNGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLFSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     ALASRMEKRSLVELLKGGIL"
     gene            complement(17410..17691)
                     /locus_tag="ACL8LA_000911"
     CDS             complement(17410..17691)
                     /locus_tag="ACL8LA_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS1812688.1"
                     /translation="MNHEKISVASLMVCSILASTSVLAVWVDGGQWNYGVGWSGNFGY
                     SDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            18103..18429
                     /locus_tag="ACL8LA_000912"
                     /pseudo
     CDS             18103..18429
                     /locus_tag="ACL8LA_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(18481..19068)
                     /locus_tag="ACL8LA_000913"
     CDS             complement(18481..19068)
                     /locus_tag="ACL8LA_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS1812689.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            19310..19981
                     /gene="sdaAB"
                     /locus_tag="ACL8LA_000914"
     CDS             19310..19981
                     /gene="sdaAB"
                     /locus_tag="ACL8LA_000914"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGS1812690.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            19990..20862
                     /gene="sdaAA"
                     /locus_tag="ACL8LA_000915"
     CDS             19990..20862
                     /gene="sdaAA"
                     /locus_tag="ACL8LA_000915"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGS1812691.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            20884..21516
                     /locus_tag="ACL8LA_000916"
     CDS             20884..21516
                     /locus_tag="ACL8LA_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1812692.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            21566..21736
                     /locus_tag="ACL8LA_000917"
     CDS             21566..21736
                     /locus_tag="ACL8LA_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812693.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NIYIRQGDVDVV"
     gene            complement(21822..23672)
                     /locus_tag="ACL8LA_000918"
     CDS             complement(21822..23672)
                     /locus_tag="ACL8LA_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGS1812694.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(23714..24871)
                     /locus_tag="ACL8LA_000919"
     CDS             complement(23714..24871)
                     /locus_tag="ACL8LA_000919"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1812695.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENVEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            24929..26095
                     /locus_tag="ACL8LA_000920"
     CDS             24929..26095
                     /locus_tag="ACL8LA_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGS1812696.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            26234..26560
                     /locus_tag="ACL8LA_000921"
     CDS             26234..26560
                     /locus_tag="ACL8LA_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS1812697.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            26547..27140
                     /locus_tag="ACL8LA_000922"
     CDS             26547..27140
                     /locus_tag="ACL8LA_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGS1812698.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            27133..28044
                     /locus_tag="ACL8LA_000923"
     CDS             27133..28044
                     /locus_tag="ACL8LA_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGS1812699.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            28107..29171
                     /locus_tag="ACL8LA_000924"
     CDS             28107..29171
                     /locus_tag="ACL8LA_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGS1812700.1"
                     /translation="MKQEWFESNNFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(29243..30103)
                     /locus_tag="ACL8LA_000925"
     CDS             complement(29243..30103)
                     /locus_tag="ACL8LA_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGS1812701.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDDSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLAQEK"
     gene            complement(30225..31211)
                     /locus_tag="ACL8LA_000926"
     CDS             complement(30225..31211)
                     /locus_tag="ACL8LA_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGS1812702.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(31483..32958)
                     /locus_tag="ACL8LA_000927"
     CDS             complement(31483..32958)
                     /locus_tag="ACL8LA_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1812703.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(33216..34139)
                     /locus_tag="ACL8LA_000928"
     CDS             complement(33216..34139)
                     /locus_tag="ACL8LA_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812704.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(34153..35082)
                     /locus_tag="ACL8LA_000929"
     CDS             complement(34153..35082)
                     /locus_tag="ACL8LA_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812705.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(35401..35622)
                     /locus_tag="ACL8LA_000930"
     CDS             complement(35401..35622)
                     /locus_tag="ACL8LA_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812706.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(35576..35836)
                     /locus_tag="ACL8LA_000931"
     CDS             complement(35576..35836)
                     /locus_tag="ACL8LA_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812707.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            complement(36262..36417)
                     /locus_tag="ACL8LA_000932"
                     /pseudo
     CDS             complement(36262..36417)
                     /locus_tag="ACL8LA_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            complement(36603..37214)
                     /gene="rpsD"
                     /locus_tag="ACL8LA_000933"
     CDS             complement(36603..37214)
                     /gene="rpsD"
                     /locus_tag="ACL8LA_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGS1812708.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(37409..38461)
                     /locus_tag="ACL8LA_000934"
     CDS             complement(37409..38461)
                     /locus_tag="ACL8LA_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1812709.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(38458..39156)
                     /locus_tag="ACL8LA_000935"
     CDS             complement(38458..39156)
                     /locus_tag="ACL8LA_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1812710.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGCETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(39323..>39655)
                     /locus_tag="ACL8LA_000936"
     CDS             complement(39323..>39655)
                     /locus_tag="ACL8LA_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1812711.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT        12119 a         8394 c         6747 g        12395 t
ORIGIN      
        1 cttcaacttt tttatccaac tcagcttcag cagcttcttt atctaattca tcctgagtct
       61 taccttcagg atcaaggctg tcaagaagtt tttcaagttc tgtttgtttt tttgctaact
      121 cagcttgttt agcgtttagc tcagcttctc ctttttttaa tttagcttct ataacttctg
      181 tgccatcatc aggatccgca ccagcaagaa gttttttcaa attatctact tgatgttgag
      241 cagtagcaac ttcttgttcc aaagtagaaa tttcatattg aagtttttca atttcaagtt
      301 ccttagcctc tacttctttc ttcgctagta ctacctttag agttgcataa tcatattttc
      361 tcttagctac tttttcttct gctttagctt cttctgcttt tttcttagtc tcagccaact
      421 gctctggctc aggaactacc attgcttgaa cagattgaaa ttttgtctga gcatcggtta
      481 gtttagtcgt ctcctcagtt atttttttat ctgcttctgc taatttttta gcgtactctg
      541 gatttcctga atgtttattt ttttcttctt gtgcttttct ttgttccaca taagcttttt
      601 gcaccgctaa actagcatca tctacttcct tagcagcttc ttctgctttt ttcgctttct
      661 cttcagtttt cttctgatcc tcatcatatt tcttctgagc gtcttctgct ttctttttag
      721 ccgtttcgta atctttcttg gcagtatcag cttttgcttt tgcttcctca tatttcttct
      781 ctaatgaaga tttttcgaca acttgtggag cttcttctgc tcttacaaaa gtaggctgag
      841 acgtaacaaa accagcccct aagatagcga cgctggctag acttgttaaa atcatttttt
      901 cttattcatc taaatttacc tcttttctga tagtcatttt tagactatac ttatattaag
      961 tatactcccc ccgtaaatat ttgtcaaatg ttttatatta catttttatt acaaatttct
     1021 atatcataag cttctactcc aaaaagataa agacactact tttgcccttt tcgattctct
     1081 ttttaaacat agcatcatct tccttcattc ctataaaaac atttccgatg agagagcttg
     1141 ttttttaatt tcgttaaaca acaaaaaacg caagcttaag cctgcggaca atctttatag
     1201 ccatttcatt tctttcacat ctgttttacg aactttagca tatttcttag cgcttgtaaa
     1261 atgtagagta actggtttaa atactccgct tttgtctctt cgtaaaccta aaactgcgga
     1321 tcttttattt ttatcctcaa aaaatatgac gtccaatttc atagtatttt tagataaatc
     1381 cttagctacg atgcaacaac ttgttatttt aaaatagaaa atatcaccta taaactcata
     1441 taaagatatc ctttcgatta catcattgta caataggata tttctaaaat cttctaaaat
     1501 aaaaacacca gcttgaatag caggttttgt ttgactagca taatctcgtg tgattttgtg
     1561 taagccaaat aaatgatgta aatcagtcag gcaaaaattc actttgaaag tctctaaata
     1621 cttataattc gttgatactt gacatgcttt tctattgaat ctttcttgat aatccaaaat
     1681 acaatcaagt aatttcaacg acaaactcca tattttctca aaataaaaaa gcacggggca
     1741 gttctcacta ctgcccaaag tggttgatta gagtcggctt ctctaactcc ctatgttgtg
     1801 cttattaggc ttatcgcaag ggaacagttt ttagctctgc caagccacat agtgtgctta
     1861 ctactatgtg tccatgtcac catgaattgc cgaacggttt aacgagaaag tatagtagat
     1921 tgaaacaaga tgtgaacaaa tcgattagga aagtcaaatt aatttctaga aatattttaa
     1981 cagtcataac ggactattcc agattcaatc tactatatta cttttaggtt tatcccaact
     2041 caattatgac atcttttttt caaaagtcaa tatatctcac tcttttcaac gacaagaaag
     2101 aggctgataa tcgaccaacc tctgttttta gatacagttc aagtccaaac tattctttat
     2161 agaaaagtcc gataaattcg agcaatagtt aacatactta caaatataac tcccaaaata
     2221 gataacacta ttttcacaaa actcagttcc gaacctatga catatatatg aaaaagaggg
     2281 gctagtccta cacatatata caataagtag ccaacaagtt ttatctcacc aattcctgca
     2341 aaaaaatgtt tcatgtaacg attctccatt tactctatta aatcaatttt attttagact
     2401 atcataacac aatcacctct tcaataaaaa ccattggcga aatactattc tgaagcattg
     2461 atagctcaga tttctcagaa tagagctgtc ctaactatcg agcagctcaa atacatatat
     2521 atgagcaatt ctaagtttag aattgctata aaataaatgt ttccaggcta ttctaagtct
     2581 ttgactgctt aaaattttac gttctgaagg attctaaagc tagaacagct caaaatcacg
     2641 agaactaagg tattcgaaac ttcagagtaa tacaatcccc ataggaattc tccattctta
     2701 ttaagcatga ttaaaatgta atcctaacaa gtgatcctcg actgtgtttg tagcacttag
     2761 ttacctagat aagactattt ccctttatca tatctgattt taagccgttc taagatttaa
     2821 cctatgtttc tattacttgc tatttttaaa taattcgaat tccttttctt tgataaatgg
     2881 aactgaaaaa ttagaaaaaa gaagtaaaac cgaaaacaaa tagcctgctt tttgaatcac
     2941 taaaagatat cctaaaaata agtaaactat aacatccaat gcgaaaagaa aaaatattct
     3001 cctcttcatt aaagtacttg tgtcaaataa tctgatgaat aaaaatttta tcgtagtatc
     3061 tacacaaatt tttatcaaaa agataagagc caatacacga ttatcaactg taccgtaaag
     3121 aatagatgga aatatagtta atataaaact agaaatctct aaatatttta tccaaggaga
     3181 ttgagacacc ttgcttctaa actgactaat tttcttatat tcgttagtca atttagtagc
     3241 aaaatcttcc ccaattaaat gaaagattgc taactctagg ataaaaacac ttattaatac
     3301 ggtataagtc aaaaaacttt ctgggaaaaa cttattgatt aaaatgggga aactcattaa
     3361 cagaatcgaa tttatggcta gacagataga tactatttta tttttcacag aatatagagg
     3421 tagtgatact attgagtaga taaaagctaa aaaaataagc aaaaaatact ccgtgtcatt
     3481 tataatcgcc ctgaatgata aaaatatata catccaacat acgataatat gtatttttcg
     3541 aatcttatct acattattca tcttactgtc gcagatataa atccatccca aggataaggg
     3601 ttcccgctaa tatcacaacc tctattaaga gcaccatatc caatacagta agctattttt
     3661 cctgctgctg caccaataag ccagcctcca agacctccaa ccttaatata gtttattagt
     3721 tttttggtta aaactgcagc tccagctaat gctataaaag ttcctgaact catagctaaa
     3781 gctacgcaaa ttccttgaca agcacgcttg cttaaataag ctcccccatc aagatacatc
     3841 atttcttctt catcaatcac aacataatta tttggtaata caagttccat ttgttttctc
     3901 ctccttagtt gaaattgttt catttgtttt acaaatagga cgaacttaaa ctatcttttt
     3961 attactcagt aataatttcc gagatttcat tttatgatac tctctattca tacagaaatc
     4021 aaaattttgc cttaaccaac ttgtataata gaaaaaatct aattgattta ttctgatgag
     4081 ctgtattcta attttatcaa ccttttcttg ccagttgtta ttgttactaa tagaaatctc
     4141 atacagcagt aactcgacaa gacctgtaca cagaagggtg ttatgagaat cttcaatttt
     4201 actcagaata tcctcaaaaa gttcactatc cgttaaaaag gtggattgat tcatcgcatc
     4261 atacagctga agagacatta agtcagaata actgagatct gatttttgta ttttatcctt
     4321 taacgtattt gtcaaatcac tttcaaacga atcttctttt tcccatttat tttgattgaa
     4381 aagaaattcc gtcttattaa aaacgagatg attgatatcc attatagtcc aaaaaaatat
     4441 tgtagtaagc ataatgatga caaaaggaat ttttaaaata aatactagga taatagcaat
     4501 ttcaagaata aaactccata taatcctaga tcttaaatca tcaggcgaca aacttttacg
     4561 atggaatgaa aaatagggcg agaacctcac atttactttc ttcctgccta taaaaataaa
     4621 aggaaaaatc gtaataagag cttctttctt gtacaaatct ttcaatgcgt atagtatgtc
     4681 taaaactatt tttgataaaa tcaaactaca gaaagcaaga ctcatactaa tcacaaaatc
     4741 aaggggagat tctagattca atagtaagtt attcactagg acatgcaact ctagccacaa
     4801 gacaaaaaga gggagcatta gtaagaaaac aattagttta cgcattataa caattagtaa
     4861 aatttacgcg tccataaagc atacgtaatt cctgcaactg aaatacctcc aattacagcc
     4921 aatgccgcat taacagctgc tactgaccct agtattggag cgaaagcaga aacaacataa
     4981 gtataagaat acactcttgt agaccatatt gcagcccata aaccagtttc tcttgcggca
     5041 aacctaaatc ctgaggaagc tctcgctaaa ccttttatat tatttttaaa tgtgttccag
     5101 tctcccccat caaggtacat catctcttct tcatcaatca caacataatt atttggtaat
     5161 acaagttcca tttgttttct cctttttatt aagcatggtt aggatggttt cctaacaagt
     5221 aatcgtcgtc tgtgtttcta tcacttagct acctagataa gatatccttt ctctacctta
     5281 tttgttttta taggttacct aaggtttaaa accaattatt gcttgaaact taacatttct
     5341 aaaatccaat caagataggt ttctttatca tagacaatcc gcacttcaac tggcatatcc
     5401 ttttggagga cgactgtctc acttccatgt tttagagtta aggtttctaa ttctatcatg
     5461 actttataaa gactaatatt gccttctttc gtttcttggg aaattgttcc tgaatcaatc
     5521 tgtctgactt gtccctttag tgttccatat ttttgactat ttacaccagt aatagcaaca
     5581 tcagcttttg ctcctttcga aacacgagaa atatcactcg caagtacaaa agcttctaca
     5641 tagtaacctt tttctttccc tgaaacttct gctatcgttt gcccctgctg aatagacatt
     5701 ccttgtttca aaggtaacag ataatgcaga gtaccttcat ttggcgctaa tatactatga
     5761 gctttatctt gacctgttgc cactccaaga ttagcctcaa gctctgtaat acttttattg
     5821 ttgtttgaac gagcagtacc taactcacta actaattgag cataaatatt ataagcctga
     5881 gaagttggat cattataggt acggttcata tccttaatat tgctttgttg actatcaatc
     5941 tcgctattct tagaagtaat atttgttttt aatgcttcga tttttgttgt tatttctgat
     6001 atcttagttt ctaacttagc tttgttatca gctgatgtcg tatctgatag agaaggtgag
     6061 tctccttgct gttgaagttg aagcaattca ttttgtagag cttgcaagtc ggcatctaac
     6121 tgatttcttt cagctgttat tttattcaac ttcgtatatt catcctgaat ctttgaatac
     6181 tgggtaccat tattataatt ttccgaattt aactgagata ggtagtattc aactttccca
     6241 taatattcct gttcttcacc agaattagat aaatgattgt atttctcatt taatgattgt
     6301 tcaaattttt gaaagatagc ttcttttgca cgtaacttct ccaattgttt atttaaagag
     6361 ctcgcttgta aaccttcttg cccacttgat aacgaaataa tgacatctcc agcatttacc
     6421 atatctcctt ctgacttatg taaagtaact accttccctg aaccaattgc tgataggaac
     6481 tctgtacctg ttataactga atttccattc gcttttacaa tatagttttt gggtatataa
     6541 gctgcgccaa ccaatgcacc gcttaagata atagcagttg aaataatgag aataaacgca
     6601 aaagctggtg gtctcttatc aaagaaaata cgagaataac gtaattctga tttattatat
     6661 aatttcatag gcttacaatt ggtctaaaaa tatctactac cattttttca ggagaagaat
     6721 taacataaac tgtatagaca atcccatccg tttgaatatc attttcatag acatatagat
     6781 ccaatttaga atacgcatac tgtagatact ctggactgtc ttcaaaacga acatataaac
     6841 aatatggaac agagatagaa tcctgtacat cataaatgtt actgtactgt tgagcattat
     6901 gagcttgaat ataaaactca aaatcagtcg ttattaatcc atcatcatga atagtagtac
     6961 cacaactttt tacaattaat ggaccaaaaa tttgtgcttt taacaactgc aaatgttgat
     7021 gaaatttatt aatttcctga tctacatctt ctacttttgt atcatgtaac tttttacaga
     7081 taactgactt tagtaccagt tttttattat cttttacctc taacttagcc ataagtaacc
     7141 tcctctgtat ctaacacagc ctgtgactga atttgttgat tcacttgaac gctctgcaaa
     7201 ccaaccattc tagcatactt tccatttttc gccattagtt cttcatgagt accatattcc
     7261 ataatagtcc cattttctaa aaagcatatc ttatcacatc gaagaattgt agacagcctg
     7321 tgggctacta caattgttgt cttatccatt attttattaa agattaaatc ctgaataatc
     7381 tgttcactaa atgaatctaa gttagaggtt gcctcatcaa atatatacaa atcagcttta
     7441 ctcagtagtg ctcttgcaat agccaatcgt tgtttctctc cacccgagaa attttgaccg
     7501 ccttcttcta caaaggagcc ataacgattt tgaagacgtt gaatcgtatc atgaatacca
     7561 acaatacgac aaactctcac catatcctca tatgtaacag aaggattacc aatttttaga
     7621 ttatcaataa ttgtccctgt aaagagttct atggtctgag gaatgtagga aattttctta
     7681 cgtagtttac catagtcaag atctgataag tcattttctc ctatacgaat tttcccctca
     7741 ctcggattta taaatcttaa taacaaacga actagcgtac tttttcctgc tccgctttct
     7801 ccaacaaaag ctattttttc tccttgttta attcgcaaat taaagtcctt gataacagga
     7861 gggcgtgaac cataggcaaa tgttacatct ttaaattcaa tgtcatcaca taaggtgaag
     7921 tctctaatac tataatccaa ggcaatgtct tcacgatcta cttccatcaa ttcttgaagc
     7981 cgactaaccg caacctgtac ctcttgaaag gttaactgca agccaactaa attctgaata
     8041 ggttctgtaa aatattgact taatgtctga aaaaccaaga ggtccccaat agtcattttt
     8101 ccatcaatga taaataaggc gcccactccc ataaataata acccacccat ggttgaggtc
     8161 attgaagata ttgtagattg aatattctgc aaaacaccct ctttataacc aatttctaaa
     8221 gtatgaacaa atttttcttc aattttgttt aaacgttgtt cctcatcatg ctgagactta
     8281 atggtatcaa tgttacgaat agattcaatt aactgtgaat taagaagtcc atttgcttcc
     8341 atttgttcat gattaatttt tttataaggc tttttaaaac agtatataag aatgatgtta
     8401 acaataacca taaaaactaa tatcagaaat aaagattgat taatagtcca aaggacaacc
     8461 gcactaataa ctgacaaggt gatatccatg actaatgaaa tagagactga cgtaaacaca
     8521 ttctttatcg tcatcgcatc ttgaaatctt gttaaaacat caccaactct acgacttcca
     8581 aaaaaactat aaggaagatg aataatatgg tcataatatc ccattaaaac cggtatatca
     8641 atttttcgag atagaaacaa aagaacgtgc tgacgaaatg cagatagaag agtctgtaag
     8701 aacgacacta ttccaaatac tatcaaaaac atatacagac tattttttaa agcatatgga
     8761 atcacctcgt ccatgataac ttttgaaaag agactcgata aaataccaat gatacttaaa
     8821 acaaaggaag ctaatatgac tgtactaatc aatccttttt gattaaaaat taaccttcca
     8881 aataaatcaa gaagtccttt tccctttagt ttccctcttt caaaatccga atttggaacc
     8941 ataaaaagag taataccttg ataaatttct cccagttcgt cacttgacat cttgcgtatg
     9001 ccactagcag ggtctgctac gtagaactta ctattccttt tgatactatg tagtaccaca
     9061 aaatgatttt gacctaaatc attcttaact tgaagaatcg caggaaatgt taattttgga
     9121 gtcaaatctt ccagtgctac tcgaacagct tttactgtaa aattcaactt attcagacct
     9181 gaaacaatac cactgacagt cgttccatac atgtctgtac caataatttc cctaatcttc
     9241 atgattgata gttccttttt gtaagataaa agaactgttg aaacaactgc cgcagcacaa
     9301 tctgactggt catgttgcat cgtacaagga tattttttta acatagaaat actcaattct
     9361 ttagaattaa tctaactttt ctttgatgtc acccaaacct caatcactgc ccagtagttc
     9421 ttgaattaat ttattttcaa ccgtatcatt ctttgttctt gatatctttc caagcattgt
     9481 agtacagcag atttttgccc aaggactgag cctcctttaa caactcaaac tcccttacat
     9541 tatatcggcc agccccatgg aaagattata gccagactaa tcaatgatat aatacatact
     9601 agaatacaaa atattttttt attcatactt tttctcctat ctttattttc tacctacata
     9661 gtacctctta ttaagctcat tttaaaatta cgttaacttt tttcacaaat ttatttcgac
     9721 tttctttttt actttttaca ttgctatgat acaatatttt taacaaaatt ttattgtttg
     9781 ttaacttttt tcacgaaaag gagaaaatca tgcgttatga tttcggaaag gtctataaag
     9841 aaatacgtga gtcaaagggg ctgacccaag aagaggtctg tgggggtgtt ctctcaagaa
     9901 ccagcttatc aaaaatcgaa agtggcaaga caaccccaaa atatgaaaat atggaatttc
     9961 ttctccgtca aatcaatatg agttttgaag agtttgaata tatctgtcaa ctttatcaac
    10021 caagtcaacg cacagagatt atgcaaacct atctcaatat gcgttccatt atcgggacca
    10081 gcgatcttgt caatttattt caaaaatgcc aagactacct caagacccac cacgatctcc
    10141 ctatagaaga aatcagagat atgcttgaag ttgttattta tctccgtcaa cacggggcag
    10201 gggaactatc aaaacacgca gaacaggttg tcaaaaaact ttggaaaaaa attgaaaaac
    10261 aagatacatg gtatgagagt gatctaaaaa tcctcaatac cattcttttc agttttccca
    10321 ttgaatacct ccatctcatc actggaaaaa tattgcaacg cctagaagtc tataaaaact
    10381 atcaacattt atatgacttg cgaatgacaa ttctattaaa cctttccaca ctctatctat
    10441 acaatcaaga taaaaacatg tgtaagcaaa tctgctacac tttactggag gacgccaaga
    10501 ataagaaaag ctacgatagg ctggctatct gctatgtccg tattgggatt tgtagggatg
    10561 atgcgaaact tatccaaaaa gggttctccc ttctggagct aaccgaggaa acttctatac
    10621 tgtctcatct caaaaaagaa gtagagatct attaccaagc gaaggaaaga taaaaaagcc
    10681 gagggatttc ctcgactctt tacatattat ttgttcgtta gttcttaata ccagccattg
    10741 ttaaaccaga agtttttagc tctgccaagc cacatagtgt gtttactact atgtgtccat
    10801 gtcaccatga atcgccgaac gatttaacga gaaagtatga cttttacgtt tatcccaact
    10861 caattatgac atttttttca aaagtcaata tatctcactt tttcaacgac aagaaagagg
    10921 ctgccaatcg accaacctct tattctgaac ccatcactcc atcacttttt agcttcattc
    10981 gctttcttag cgactgcaat ctggtattcg acttggtcaa accctgtgcc tcccaaggaa
    11041 tttcgtcttt gaacagcagt tttaggttgc aagtagacat agatatcctc ggtaatgaga
    11101 gaatgatagg tttgtaattc ctccaaagtc caatcttgaa gatttttagc agacttgata
    11161 gagtctagca ctaatcttcc cacaacctca tgagcttctc taaatggcaa gcctttccct
    11221 gccaaataat ctgccaactc agttgcattt gaaaagtcct tctctgttga ttcttgcatt
    11281 ttttccttgt ttacctgcaa actagataac atacctgcca acacatcgag ggaatttaaa
    11341 atcgtttcga ccgtatcaaa cattccttcc ttatcttctt gcaaatcctt attataagct
    11401 aaaggcaagg acttcatgac tgtcaacagt ccaaacaaat gcccataaac tctgccagtc
    11461 tttcctcgaa tcaactcagc catatctgga tttttctttt ggggcataat agaggaacct
    11521 gttgtaaacg tatctgacaa ggtaatgaat tggtactcaa aactacacca attgatcatt
    11581 tcttcgcaaa aacgactcat atgcatcatg agtatgctgg catttgatag aaattctaag
    11641 ataaaatcgc ggtcactaac agcatccaaa gaatttgtat aaggttgctt aaactccagc
    11701 aaatcgctag acaattggcg atcaatgggg aaagtcgttc ctgccaaagc tgccgcacct
    11761 aaagggcata ggtctgtatg cttctggtta aattcaaaac gttcactgtc tctttgaaac
    11821 atattgtagt aagccatcag gtggtaggca aaactaatag gctgggcatg ttgcagatgg
    11881 gtatagcccg gcatgatcgt cgccacatga ttttccgcca agtctaataa aacaccctta
    11941 agatgagcca gtttatctag gacatagcct agttgttcct tgagatacaa gtgcatatct
    12001 gtcgcaactt ggtcatttcg agaacgagcc gtatgtaact tccctgcaag gggaccaatt
    12061 ttttctgtca gcaacacttc catattcata tgaatatctt cgtttgcgat atcaaagtca
    12121 agttgacctg cctctagctc ttctaaaagc tctttcaagc ctacttgaat cttttctgac
    12181 tcctccaaac tcaaaatgcc cgtctgaccc aacatctgaa cgtgggctaa agaaccaatc
    12241 acatcaaatt tagctaattt ctggtcaaaa gatatactcg caccaaagcg ctctacccag
    12301 tcttcgacag taccttcaaa gcgaccaccc cataattttg tattcttagc catatcacat
    12361 ccacctctct atccaatcgt cctatttggc gcttttttga acctcagaat gaaccttggt
    12421 tggaagtccc cataactcaa taaagccaac agccgcatct tggtcaaagg tatctgcact
    12481 agtatacgtc gccaaatttt catcgtaaag agaatttggt gatttccgag ccacgacctg
    12541 agcgctcccc cttatacagt ttgacttttg cagtcccatt gacaactttt tgagtctctt
    12601 taatataagc aatcaaggct tgtgtagccg aactaaacca caaagcatta taaatgagat
    12661 ttgataattc attttctatg attggtttaa aatgagccac ttctctcaca agcgtcaagt
    12721 cctcaatttc cttatgagct gtcagcaggg tcactgcacc tggacactca tagatttctc
    12781 ttgatttaat cccaaccaag cgattttcca cgtggtcaat ccgtccaacc ccatgttttc
    12841 cagctatttc attgagtttt tgaatcaaat ctgccaattt taacacttct ccattgaggg
    12901 aaactggaac accttcacta aactcaatct caatgtattc tggcatatct ggagcttgct
    12961 ctggagaagt tgtgatgcca aaggcttctt ctggagcctg attccatgga ttttccaaaa
    13021 taccacactc attggcacgt ccccaaagat tttggtcgac agagtaggga ttgtcaaggt
    13081 cagcaggaac aggcacccca tttttcttgg cataataaat ttcttcctcc cgagaccatt
    13141 tccattcacg aactggagca atcactttta aattgggatc caaggctgca atcgatactt
    13201 caaaacgtac ttggtcattc cccttaccgg tacaaccatg agcaattgta gtcgctccta
    13261 tctgatgcgc tatttcaacc aattttttag aaatcagagg gcggctcaag gcagatacca
    13321 agggatactt ttgttcataa taggcatgtg actgatgagc cactagcaca taatctgtag
    13381 caaattcgtc cttaacatca atgacataag attctactgc cccaacctta agagccttat
    13441 catggatgaa atctaagtct ttcccttcac ccacatccat acaaactgaa acaacatcat
    13501 agtctttctt taaccaggta atagcaactg atgtgtccaa accgccagaa taggccaaaa
    13561 ttactttttc tttactcatg ttcttttcct tctttctatt tttcaatgga tgctttaaag
    13621 gcatcctcaa tgagtttgtc taattcccca gactccttca acttttgaat agttttatcg
    13681 actgcctctt tcaattcctt actatctttt ttcatggcta ccgagtagga atcatcttgc
    13741 tctttttcaa aattgaggtc tgcgattgct aaatcaggat tattttccac aaagcccttg
    13801 gcaactggtt cttcaaagat aacggcatcc acttgtcctg attttaaatc tgtgattaaa
    13861 ttcccatttt taggcagaga tacgagggaa gaattttgta gcaaatcttt cgccatcgtc
    13921 tcttgaatcg aacctttctg ggctccgacc ttcttctgcg ctaagtcgtt gacagactga
    13981 taagtgacta aatcagattt tttgacaatg agtttatttt ttgcagtata gtagggaatg
    14041 gaaaagtcaa acaccttgct ccgttcatct gtcttagaaa cacctgatat ggcaagatca
    14101 gcttttcctg attgaacact agccagtaca ttgtcaaaac tcatgggaga tagttccaat
    14161 tcgacaccta gttctgttgc gatagccttg gctaattcga tatctgaacc cacaatctga
    14221 tttttcccat caaccacttt ttggtattca aatggagcaa aatctggatt gagggctaca
    14281 accaattttc ctttggactt gatggcttca attccagccg attgattgtt actacaagca
    14341 aatagtaggg ggagcatcac taaaccaaac atcgtcatca acaccttctt catcttattc
    14401 ataacaaagc ctcctttatg tttattcctt ttgtttgtat aaataataca cctatcttca
    14461 tcctttgtca agcctaaatt gtaatttttt tcaatttaaa acagaaaaac ctaggaaaat
    14521 tacataaaaa tgtcattcct aggcgtattt atgctattcc tctctggaaa atatgagtat
    14581 tcagtcagtc aaaagaagct gaacgaactc attttccctc gcccaattca ataatccgat
    14641 gacattgttg ggctacataa gcatcgtggg tcacaataat gactgttttc ccttctcgat
    14701 tcatctctaa gagaaacttc aagaccaaat ctctattttc aggatccagc gaacctgtag
    14761 gttcatcggc taaaatcagc tggctgggtt ttaagatggc tctagcaact gcaattcgtt
    14821 gttgttcgcc accagacaac tcggagacct tttgatgcaa agtagctgac aaacctactc
    14881 tctctaaaat ctcttccacc tttttgagct tatctttctt agacaatttt acatatttca
    14941 gcgccagcat aagattgtac tcgaccgttt catcatcaat cagggcaaaa ttttgaaaca
    15001 gataagagat atgttcacgg attattgttt gcgacttagc agaattgacc gctagatttg
    15061 tctgaccaaa aatctcatac cgtccgctat aatcaccatc tatcaaaccc aataaattta
    15121 acaaggtcga cttcccacta ccactcttac caacaatggc aaccaaatcc ccctgatcaa
    15181 tcctgagaga caagcgatcc aaaatcactt tccccccaat ggttttggta atattttcca
    15241 actcaatcat aagatgcccc ctttcaataa ctctactaga cttcttttct ccatcctaga
    15301 agccaaggct agcacaaata gtatatccaa acatgtaaag cctgcaaaca gcaggagtgg
    15361 caagaacgca tgggcaaaga aaatcaagac tagaagaggg agactatagc ccagcaagag
    15421 cagaacgagg agaggacggt agcgatcgac cagtttccac cccataaact tcttggtaat
    15481 aatatccctg cgcttcaata agaaagttat tactagtaag aagtaggaaa tcatcatgct
    15541 aaagagacca aacaaagcaa aaagtaggtt aaaattccga acagcatctc gataagaatc
    15601 cactttctct tgttgaatgg cttgaataga tgaaaatttt aaataatttc catctgacaa
    15661 tttctcaact aactctgtaa tctctttttg atgttgaacc gtattttcaa ttttaatcgg
    15721 attatttaag ccagttgttg acagggaggc tttctcatcc cacatcatat cagaatcatt
    15781 gaccaagcta ataattggat tggagagatt ttcctttcgc ttatcactat atgggaaaaa
    15841 tgaccaatct ccttcataat aggcaatctc gacatccatc tcctctatcg ttcgtttttg
    15901 ctgctcttca tacttcatcg aatgaaaggc aattaacttc cccaagagct gatttttatc
    15961 ttcttcacct ttcgtacttg ctggcatcaa aataactttt ttaataccag tatctggtag
    16021 cttgaatccc ttgctcttta gaaaattgcg attggcatag taaacatcca ccgtatctgt
    16081 taactgatat tgctgaatct gttctgattg gacaaaattt tttacaggaa gactgctact
    16141 ctgcacatag cccgcctgcg ttttttctac taaatcctga taaaatcgat agaaataatc
    16201 caatgccttc cctgagcctg ccaactgctc ttgccacaag ttgtcattga gttggaaagt
    16261 ttctaaggtc agataatttc cttggctgat ccactgtttc tggtaatcca actctttgtt
    16321 ttcttgctcc aaactttttc ctaccccaac tagcaagact gtcagtaaaa tggtagttcc
    16381 aattttcatc aaataattaa agagtagacc aaatttgaaa gatgaaaaac ctttcagcag
    16441 agagctgatt gtcatttttt ggattaagag gtaagtcaac caactgataa agagataaag
    16501 ctgcaacagc aaaaaatgag acaaccacag cataggaaac aaatcttttg gcttataatc
    16561 aagcaagaaa aacacgccta gattgatcac aagagcccca cctaggagga ggtaaaggtt
    16621 gccttttaca acatcagcta aaacagccct atcttgaaaa ccaagtaatt tttgtacccc
    16681 aactcgtttc atctccatca tcggttgata cactgtcact aacacaagaa gcaaaatagc
    16741 caagacaaaa acaatggcag ataaaagcaa atctcgattt atgacttcca ctgcactttt
    16801 gtaggtcggc tctagcaagg tagcctggtc tatcttgaaa aaatcgctcc atttctgtac
    16861 aatcctatcc ttgtccatct cttgtgtaga agttatcgta tagcgaccat ttaaactacg
    16921 agatgtatcc ttgatatagg tttgaaaagt cataagctga ataggtttgg cttttagaaa
    16981 ggtcggaatc gtaccaagtt tattggaaat ttctttatta ctatagactc cttcaccatc
    17041 tgtggtaaaa tcaagagaag aaatcccaaa ctcttggtag gggaaggtat ctttatcaaa
    17101 aacaccagac ttgaccacct catcgccatt atctactttg ataatagaga ccttgtcttg
    17161 gcttgcaacc tcttcaaaaa actggagaat cgtttctgtc ggtttagtga catctttcaa
    17221 gtacaagtca aatgaataag ttgtcttgcc catctcctga atccgcacta tttctcgtgt
    17281 aacatttaca ttcaggacaa aaaacgtagt acacagcaag agcaggagca tacaaagatg
    17341 actgattttt ttcatagaga tacctcccgt gaagaaactc cctctccaaa aggggaggga
    17401 gaattttaat taaaatccat agaaatattc cattcccgtt ggtgggaatt tgaagattga
    17461 agcccgagcc cacgcctcag cttctgcacg atccttagag aatttatttc catctttaac
    17521 agttgctgta tgagagcgag tagaatgtaa ataatcagaa taaccaaaat ttcctgacca
    17581 tcctactcca tagttccatt gaccaccatc aacccatact gccaaaacac ttgtagaagc
    17641 aagtatacta cataccatta aagatgcaac actaattttc tcatgattta tttctcctat
    17701 tatcgagagc tattccgtag aaacagctat agtaatttat caacaagtat cgtagtccat
    17761 tggactcacc ttccttctat ctcatttcat ttttaaacaa cgaacttact cttaactagg
    17821 agaatagttc tcttctgtat caggtctttg aacaaccaga gtagtattaa tcacactcta
    17881 actattactc aagaatcagc ccaataaaca atatggatag cgctttcaaa tttttaaact
    17941 ctatcccatc cttttatcta tattatgagc gaaaatataa taactgtcaa gtaatcgaag
    18001 tgaattttat aaaaaaaatt acaagccaaa tttgcaaagt ttacaataag ccgctagggc
    18061 aatcgtctat cagaatgtcc gtttatttgt caataatccg agaaaatctt gcaacgctta
    18121 gaagtctata aaaactatca acatttatat gacttgcgaa tagcaatcct gctcaacctt
    18181 tccacactct atctatacaa tcaagataaa aacatgtgta agcaaatctg ctatacttta
    18241 ctggaggacg ccaagaataa gaaaagctac gataggctgg ctatctgcta tgtccgtatt
    18301 gggatttgta cagacgattc taaacttatc caaaaagggt tctcccttct ggagctaacc
    18361 gaggaaactt ctatgctgtc tcatctcaaa aaagaagtag agatctatta ccaagcgaag
    18421 gaaagataaa aaagccgagg gatttcctcg actctttaca tattatttgt tcgttagttc
    18481 ttaataccag ccgttgttaa gccagaagtt tttagcagct gtccatgaac cgtaacgtcc
    18541 tgcaacgtag gcatctgcta cacgttcttg gttttcagct gagtagtcac cgttcaggta
    18601 tgaatctgtt aattggtaac gtccgatata acgtccattt gtagctgtat agctaccacc
    18661 tgattctttt tgagcaatcc attctttggc ttctgcttca gatccgctta cagttgaaac
    18721 aactgtttca cttactactg gagtttctgc tactggagct gaaacagttt catcttgagc
    18781 ggctggtgcc gcataagtag ctggcgctgg tgttgcaaca ggcgctacag ggccatcgat
    18841 aaccaactct tgaccaacat aaatcaaatg aatgttatca atgtggttgt tttctgccaa
    18901 tttttcaact gttgtgttgt gagtttcagc gatttctgaa agtgtatcac cttctttaac
    18961 agtgtaagtt gatgattctt gagcagatac aaatgatgga gcaaatactg caaacaaggc
    19021 agctactcct gcaagagttg ctttaatctt tttagttatt gatttcatat tcgaaaattc
    19081 tccttctttc tatagttcta tcataccctt taaatattac cgtttcttga cacttttatg
    19141 tagaaatatt acaaaattat tttttatttc cctaaataag gtgttttttg tcataaaaga
    19201 tactattttt atgctatttg aggtattaga aaggttttca tccacaatta aagccaagcc
    19261 cctcattctt tgatataata gagataagaa tttttataag gggaaactga tgaaatcact
    19321 tcgttttcaa tctgtctttg atatcatcgg accagttatg attggcccat ctagtagcca
    19381 tactgctggt gctgttcgta ttgggaagat tgtctcttcc atttttgatg atactccgac
    19441 agaagttgaa ttccaacttt ttaactcatt tgccaagacc tatcgtggtc acggaacaga
    19501 cctagccctt gttgcaggta ttttgggaat ggatacagat gatcctgaaa ttccaaatag
    19561 tctggaaatt gcccacaagc gtggtatcaa gattgtctgg accattcaga aagacagcaa
    19621 tgcccctcac cctaacacca ctaaaattac tgttaaaaat gcccacaaga ccatcagcgt
    19681 gactggtatt tctatcggtg gaggaaatat tcaggtaacc gaactcaatg gttttgccgt
    19741 ctctctcaat atgaatacac cgactatcat catcgttcat caagatattc caggtatgat
    19801 tgcccttgtt acagaggcgc tttcccgcta tggtatcaat atcgcccaga tgaatgtcac
    19861 tcgtgaaaaa gctggtgaaa aagccattat gattatcgaa gttgacagtc gcaactgtga
    19921 tgaggctatc gaagaaattc gaaaaatccc tcatctccac aatgtcaatt tctttaaata
    19981 ggaggaagca tgttttattc tatcaaagaa ttggtcgagc aagcagatct ggactttcaa
    20041 ggaaatgtcg cagaactcat gattacaaca gagtttgaat tgaccggtcg cgaacgtgaa
    20101 gaagtcttcc ttctcatgga acgcaatctg gaagttatga aagcctctgt ccaacttggc
    20161 ctcaatgaaa ataaatctcg tagtggcctg acaggtggag atgctgccaa attggatcac
    20221 tacatcgaaa acggaaaaac tttatcagat tacacgattc tctctgctgc ccgaaatgct
    20281 atcgcagtca atgaacacaa tgctaaaatg ggcttggtct gcgctactcc taccgcagga
    20341 agtgccggtt gtctcccttc cgttctcact gctgctattg aaaaattaga cctcagccac
    20401 gagcaacaat tggatttcct ctttgctgct ggtgcctttg gactagtcat cgcaaacaat
    20461 gcctccatct caggtgctga gggtgggtgt caagctgaag ttggttcagc ctctgctatg
    20521 agtgctgccg ccttgactct ggctgcaggt ggaacacctt atcaggccag tcaagctatt
    20581 gcctttgtca ttaaaaatat gctaggcctc atctgtgacc ctgttgcagg tttggtcgaa
    20641 gttccctgtg tcaaacgtaa tgccatggga gctagctttg ctttcatcgc agcagacatg
    20701 gccttggcag gtatagaatc taaaatccct gtggatgaag tgatcgatgc catgtaccaa
    20761 gtaggagcaa gcatgccaac tgcctttcgt gaaacagctg aaggtggact cgctaccacc
    20821 cctactggtc gtcgcctcca aaaagaaatt ttcggagaat aagcttatca aataggagaa
    20881 accatgacct ctatcacagc gatttttttc gatttggatg gaaccctcgt tgatagttct
    20941 atcgggattc acaatgcctt tacctatacc tttaaagagc tgggggtgcc tagccctgat
    21001 gccaaaacta ttcgtggttt tatgggacca cctctcgaaa gtagttttgc gacctgcctg
    21061 tccaaagacc aaatttctga agccatgcag atatatcgtt cttactataa ggcaaaaggc
    21121 atctatgaag ctcaactctt tcctcagatt atagacttgc ttgaggagtt atcgagcagt
    21181 tatccactct acatcaccac gacaaagaat acatctactg ctcaagacat ggccaaaaac
    21241 ttggaaatcc atcatttctt tgatggcatt tatggttcta gccctgaagc accccataag
    21301 gcagatgtca ttcaccaagc cttacagaca catcaactag caccagaaca agccatcatc
    21361 atcggagata ccaagtttga tatgctggga gctcgagaaa caggtattca gaaattggcc
    21421 gtcacttggg gatttggaga gcaagcagat ctactaaact atcaacctga ttatatcgct
    21481 cacaaaccct tagaagtttt ggcgtatttt caatagcata gactgggcga caactctatt
    21541 tcagaagaaa atgaagtaat actcaatgaa aatcaaagag caaactagga aactagctgc
    21601 gggctgctca aaacactgtt ttaaggttgt agatggaact gacgaagtca gctcaaaaca
    21661 ctgttttgag gttgtggata gaactgacga agtcagtaac atatacatac ggcaaggcga
    21721 cgttgacgtg gtttgaagag attttcgaag agtataagtc tctacataat aaaacgcata
    21781 ttaccaaggt tgtgtaactt gatactatgc gtttttcttt tttattcaat gtgggttgtt
    21841 tgattagcaa aggcgataat agcttgcttc aactcatctc cactgagagt gcggaactct
    21901 ccaatctttt gattgatgga ttctaaaggc atgacattaa ccttaccaac gaaaatctta
    21961 tccataactt gattatcaac gagttcggtt gataccaaga gttcttcgta gagtttttca
    22021 cctgggcgga ttccaacttc aacgattgga atttcacttt cagtgtggcc acttagaagc
    22081 accatcttct tggccaagtc ataaatcttg actggtttgc ccatatcaag gataaagact
    22141 tccccatctt tggcataagc accagcatgg ataaccagac ggctagcttc tggaatggtc
    22201 ataaagtaac gggtcatgcg gaagtctgtc accgttacag gcccaccttc agcaatctga
    22261 cgttcaaaga ctggaatgac actaccacgg ctaccaagaa cattcccaaa acgaactgca
    22321 cagtaggttg attggctacg ttggttaaag ccagtgacaa tcaactccgc cacgcgcttg
    22381 gttgctccca taacatttgg tggattgact gccttatctg tcgaaatcat aaccatctta
    22441 gacactttag cttcatcaac agccttagca acattgtaag ttccacggat attgtttttg
    22501 aaggcttctt ttggattgcg ctccatcata ggaacatgct tgtgggctgc cgcatgataa
    22561 acaatagcag gtttgtactg ctcaaagact tgcaacaaac gatcatagtc ttgaatgtcc
    22621 gcaatcacag gtacataatc aatcccttgg aacttacgaa tcaattcatg ataaacaagg
    22681 tagattgagt tttccccatg accgagtaag acaatgcgtt caggattgaa gcgactaact
    22741 tgacgacaga tttcagaacc gattgaacct ccagctcctg tgactaagat ggtcttacct
    22801 gtcagttctg cacccagacg cgattcgtca agacggattt cctgacgacc caaaaggtcc
    22861 gtaatatcaa ttttttggaa gccagtacct gcttggtgaa ggccctgaac aacagtttca
    22921 accttaggca tcttgtaaca tttgacaccc agcttattac acatctgcaa gatacgctca
    22981 tattctgacg gatccagcga cggaatcgca acgatgacac gctcgatttg atggcgtttg
    23041 gctaattcag gcagattgtc ataagagccc aaaacaggaa taccaccaag tttttgaccc
    23101 tttttcttag aatccttatc caaaataccg accagttcta attcactggt tggatgttgg
    23161 taactatcca taaagagagc cccaccatca ccggcaccaa tcaagaaggt ccgacggtgt
    23221 tctccatcac cactaccttt tttgcgtctg gagtagatta actgccaagt aatccgtggc
    23281 aataaaatca agaaggtact caacaagata aagagaatga tgaaacggat ggagaagagt
    23341 ggcaagaagg cataacagat actatatgac aagacactgc tagcagtcac accaaaaaag
    23401 attttcatga aatccgtaat cttgctgtaa cgactaatgc tcgcgttcaa cccccaaaaa
    23461 ccaatcatca attgatagaa caagaaggcc aaactcgtat agataatgta gtcaacaggt
    23521 gctggattaa tcagcccata aaataaaata taagatacaa ttatggaaac caccatactg
    23581 aaaatatcaa atattcccca gaaaacctgc ttttgttgtt tatttaaaat ttccaccaga
    23641 tcaatcacat aatctgttag ttttttattc atagaaatct actcctcctt aaaagagttt
    23701 tactatattg ttattatagc atatttttat ttatttggct tattttatcc aaataaatta
    23761 ttgttctctt gtactccttc ttccatttaa atcctctaat taaacctaca atcgtcccaa
    23821 ggccataagc aaagtgaatg gaaaataaaa gaaagggcat cacaattaga aatccatttt
    23881 tatgttttaa taaagtcagc aaagtgagta atgacaaaag tagaaaatag gcacctaata
    23941 gtaaagttat gaatacgaat gtgatcggta acaatgctag actaaacaca agactcaaaa
    24001 caaataaaca aggaacatag tgaaataatg ataaacactt aaactgaaca tgacttgtca
    24061 agccaatcca caaaccattt gaatactttt gatgcagcat tttcttgaat gttggtcgaa
    24121 tatactgata agatagaata cttgggctat agcggatttt ataaccatgt tctcgaattc
    24181 tataatgaat atcattatct tcagttcggc caagttgctc atttactaaa ccaaccttct
    24241 ggaaaacctc tcgtttatac atcccatgaa aaatagaaga aacatatcta tcctcggaac
    24301 tatttcgata attggcaatg ctactgccaa acatattttc ctcaacaaga tgcaaggtct
    24361 ctgcccattt tccttttcct tcgacaatcg tcggtctagg ccccccacag acaaattcac
    24421 cttgttgaat aatagccaca ttgttcatta caaaactctc agtaactttt gaatgagcat
    24481 caatttttaa aataaggtcc cctacagaat gtttaactcc caggttaaaa ccactagctt
    24541 gatttttctt aggattgtta tacaatctaa ttgagttaaa ctctgtatct tcctttataa
    24601 attgctgaat gatagctgtg gtcccatctg tggacatagc atttataaat agaatttcaa
    24661 cattctcttt aggataggtt tgatttttta agtcttcaat tagaccaggc agatattttt
    24721 cttcattgta tgcactgatt acaatggata cgagaagact ttcaacattt tgagtcacta
    24781 ctactctcct gaatttttcc ttccaccatt ctatcataat tttcaagact ttcccatttt
    24841 tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa aaagccttaa aataagctgt
    24901 tattaaaatc agctagaaga ggtattatat gaaaaagcaa tcactctttt ttgttccagg
    24961 tattatcctg attggtgttt ccttgcgaac tccttttact gttttaccca ttattttggg
    25021 aaatatttcg caaggtctgg aggtagaagt tagttcgctt ggtgtcttga ccagcctgcc
    25081 tctccttatg tttaccctct tttcaccatt ttctacccga ctggctcaga aaatcggctt
    25141 ggagcatctc ttcacctaca gcctcttctt cttgaccatc ggctcactca ttcgactaat
    25201 caatctgccc ctgctctatc taggaacctt gatggttggg gcaagtgtcg cagtcatcaa
    25261 tgtcctgctt cctagtctta tccaagccaa tcaaccaaag aaaattggtt ttctgaccac
    25321 cttatatgta acgtctatgg ggattgcaac ggctctggct tcctatctag ctgtgcccat
    25381 tacacaagcc agttcttgga aaggacttat cctcctcctc acgttactct gtctagcaac
    25441 ttttttggtc tggctcccaa atcaccgcta taatcataga ctagctccac aaaccaaaca
    25501 aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg gcaattatta tcttttcagg
    25561 ttttcaatcc ttgatctttt acaccgtcat gacctggtta cctaccatgt ctatccatgc
    25621 aggtctatcc agtcacgaag ctggcttgct gacttctatc ttatctctga ttagcattcc
    25681 tttttcaatg accatcccaa gcctgacaac cagtttatct actcgcaacc gtcagctcat
    25741 gctcactctg gtttcactag ctggtgtggt cggcatttcc atgctctttt tcccaatcaa
    25801 taatttcatt tactggctta ccatccatct cctcatcgga accgcaacca gcgccctctt
    25861 cccttatctc atggtcaact tttcactcaa gacaagcgcc cctgaaaaga cagcccaatt
    25921 gtccggccta tctcaaacag gaggctatat cctagcagcc tttgggccaa ccctctttgg
    25981 ttacagtttt gacctgttcc actcttgggt accagctgta gctgccctct tgctcatcta
    26041 tatcctgatg actgtggccc tctttacagt ggacagagcg gataagatcc tttaaacttc
    26101 tagttttagc tagaagtttt ttatatataa aaattttaca catttctctt gacagggctt
    26161 tcttttagat gtacaatgta tgtgtagaaa aattatatat aaaaatctta cacattagaa
    26221 aaggaggttt cccatgtact ttccaacatc ctctgccttg attgaatttc tcatcttggc
    26281 tgtactggag cagggtgatt cttatggtta tgagattagc caaaccatta agctgatcgc
    26341 taatatcaaa gaatccacac tctatcccat tctcaaaaaa ttggaaggca atagctttct
    26401 gacaacctat tctagagagt tccaaggtcg catgcgcaaa tactactcct tgacaaacgg
    26461 tggtatagag cagctcttga ccctaaaaga tgaatggaca ctctatacag acaccatcaa
    26521 tggcatcata gaagggagta tccgccatga caagaactga atacctgact cagctagaac
    26581 tctatctcaa gaaactacct gaagctgacc gtatcgaagc catggactat ttcagagagc
    26641 tctttgacga tgctggagtc gaaggagaag aagaactcat cgctagtttg ggaactccca
    26701 aagaagcggc ccacgaagtt ctatccaatc ttctcgataa aaaaatcaat gaagcacccg
    26761 ctcaaaaaaa taaccgacaa attttacata tcgccttgtt agccctcctt gcagcaccta
    26821 tcggcattcc tctgggaatc gccatcctcg tgaccctgtt cgcaatcctt gtagccgctt
    26881 tgactgtcat tctggctttc tttgcagttt ccatactggg tatcatcggc ggattcctat
    26941 ttttagttga aagtttcact gtcctcgccc aagccaaatc agcctttatc ttgatttttg
    27001 gttctggttt actggctatc ggtgcttctt cgctagtttt acttggcatt tcctatgtaa
    27061 ctcgcttctt cggtctactc attgttcgtc tggtacaatt tgttcttaaa aaaggaaaga
    27121 gaggtaacca gcatgcgtaa atggacaaaa ggatttctca tctttggtgt ggtgactacc
    27181 gttatcggct ttatcctgct ttttgtaggt atccaatctg acgggattaa gagcctactt
    27241 tccatgtcca aagaacctgt ctatgatagc cgtacggaaa agctaacctt tggcaaggaa
    27301 gtcgaaaacc tagaaattac tctccaccaa cacacgctca ccatcacaga ctctttcgat
    27361 gatcaaatcc acatttctta ccatccatct ctttctgctc accatgatct tatcaccaat
    27421 cagaacgata gaactctgag tctcactgat aagaaactgt ctgaaactcc gtttctctct
    27481 tctggaattg gtgggattct tcatatcgca agtagctact ctagtcgttt tgaagaagtt
    27541 attctccgac taccaaaagg gagaactcta aaagggatca acatctcagc caatcgcgga
    27601 caaaccacca tcataaatgc tagccttgaa aatgcgaccc tcaatacaaa cagctatatc
    27661 ctccgaattg aaggaagtcg tatcaaaaac agtaaactca caacacccaa tatcgttaat
    27721 atctttgata cagttcttac agatagtcag ctagagtcaa cagagaatca cttccacgct
    27781 gaaaatatcc aagtccatgg taaggttgaa ctgactgcca aagattatct cagaatcatc
    27841 ctagaccaga aagaaagcca acgaattaac tgggacatct caagcaacta tggttctatc
    27901 ttccaattca caagagaaaa gcctgaatca agaggtacgg aattaagcaa cccttacaaa
    27961 actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat ctgatgataa tattgatcta
    28021 atatccacac caagcagacg ttgacaaaaa cgcttacatc tgctactatg gaagcatatt
    28081 tatctaacta aaaaggaggt tctaccatga aacaagaatg gtttgaaagt aataattttg
    28141 taaaaacaac aagcaagaac aagcctgaag agcaagctca agaggttgca gacaaggctg
    28201 aagaaacgat agccgatttc gatacaccaa ttgaaaaaaa tactcagtta gaggaggaag
    28261 tctctcaagc tgaagtcgaa ctggaaagcc agcaagaaga gaaaattgaa actcctgaag
    28321 gcggtgaagc gagaacagaa atagaagaaa agaaggcatc taattctact gaagaagagc
    28381 cagacctttc taaagaaaca gaaaaagtca ctatagctga agagagccaa gaagctcttc
    28441 ctcagcaaaa agcaaccacg aaagagccac ttcttatcag taaatcttta gaaagtcctt
    28501 atatccccga ccaagctcca aaatctaggg ataaatggaa agagcaagtg cttgattttt
    28561 ggtcttggct agtggaagcg atcaaatctc ctacaagtaa gttggaaaca agtatcacac
    28621 acagttacac agcctttctc ttgctcattc tgttttctgc atcttccttt ttctttagta
    28681 tctatcacat caaacatgct tactatggac atatagcaag cattaacagt cgcttccctg
    28741 agcagctagc tcctttaact cttttttcta tcatctctat cctagtagcg acaacactct
    28801 tcttcttttc attcctcttg ggtagtttcg ttgtgagacg atttatccac caggaaaagg
    28861 actggacgct agacaaggtt ctccaacaat atagtcaact cttggcaatt ccaatcttcc
    28921 tcactgctat tgctagtttc tttgctttct ttgatagcct acgatttaca gctctcttgt
    28981 gtgtgattag cattggaatc attctgcttg ccagtctcca tatcattaca agaccaagtc
    29041 aagcaagtga aaccgactcc ttctatcaat tattcttgtc tgtccttgtg aacggagtta
    29101 ttatcctcct cttctttgta gctgaagtgg cactgattgg agattatctt cgtatcttgg
    29161 cctttcttta aaattcaatc ctgtcatcct tggcaggatt ttttctatgt caaaaaagca
    29221 agtcgattga cctgcttctg ctttactttt cttgtgctaa tgttttaaaa tcttgtacta
    29281 ggatgggttg tatttctatt tgattggcgt ctagttggtg gtaaaaagat gggggtaata
    29341 cctctaagaa ttttgcatag tccaagcgag ctatggattc atagtcttct ggtttagagt
    29401 cgtctcctgt gatttgaacc aagtcaatct cccactggac ttccttatcc accaattgct
    29461 caatataggc tgcaggaaca aacggttctc tcggtaaaac tgtcttgact ccttgagcta
    29521 gatggtaaac gccatgcact tttccttctc catcctgata gaaggaaact ctcgcaaagt
    29581 aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa ctgagccaag ccatctgcct
    29641 ctaaaaagct tttcaagttc tcacgactaa agaaaatagg attaaaaatt ccttggcaaa
    29701 tcgtaaaacg agctacaggg tctgctagat aggcttgaat acttgcttgg aaataagctt
    29761 caaaatcaaa accatctagc ccttcaatat cagtccctgt ataagcaagc aaagcatcta
    29821 aaaatgactt gataatctgc cagtctgtct ttgttagtag gtcaggaaga tcgacacggt
    29881 aagccttttg accatcagca taactgacct taaaaagaaa ttgtgattgc cccactatcg
    29941 cacactcaat atactcgaga cgattaagag gctgacggag atagactaca tcatagctat
    30001 gtgactccaa accgtctagc aaggccaaga tcgacttggc attcaaaatc tcttgttctc
    30061 ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc cataactgga ctcctttgaa
    30121 atcgtttaca tacagtatac cttatttttc tgaaagatac aaaaacaaac tttagatttt
    30181 tgagtacaaa gcatagaaaa acagcccctg aggactgttt gatcttatac agtagctgcc
    30241 tgatccaagc tttcaccgat agcggctagg cgctcaatca cttcagcttg tgtcaattca
    30301 ttttttgaaa catagcggtt acgtgggtga acacggcact cgtgtgagca tccacgaagg
    30361 tacttgtctt cattttcttc tgatgtcaag atacgacggt tacagaaggg atttccacag
    30421 ttgacataac gttcacatgg tgttccatca aaccagtctt tccctacgat ggttgggttg
    30481 acatggttga catcaacggc gatacgctcg tcaaatacgt acatttttcc atcccaaagc
    30541 tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc ctccgtgcaa ttggccgaca
    30601 tctttgtagc cttcacggac catccagcct gagaatttct cacagcgaac gccacctgta
    30661 cagtaaacca cgacacgctt gtccatgaat ttttccttgt tatcacggac ccattgtggt
    30721 aactcacgga agttgcgaat atctgggcga atagctccac ggaaatgtcc taggtcgtac
    30781 tcataatcgt tacgtgtgtc aaggacaacg gtatctttat caagaagcgc ttctttgaac
    30841 tcttttggag acaagtaagc acctgttgtt tcaagtgggt tgatgtcatt gtcaaagtcg
    30901 ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc gaacaaacat cttcttgaag
    30961 gcttgttcat tttcttcgtc aatcttgaac cagagttctt ccattcctgg aaggctgtga
    31021 acgtagtcca tgtatttttg agttgtttca tagtcacctg aaactgttcc gttaattccc
    31081 tcgtcagcga ctaggatacg gcctttaagg ccgattgatt tacagaaagc caagtggtct
    31141 gcagcaaatt gctctgcatt ttcaattgga gtataaaggt agtaaagtaa gacacgaata
    31201 tcttttgcca taagatttgt tctcttttct attcttaaat tttctaaatt ttttatttat
    31261 ctacactagt atacctgctt gagaaaacga atgcaattta tttgactgga aattgtagag
    31321 ggttctcgaa atttcctgaa tggttaaaat aaggacaaga gaaaacatgg atatctatat
    31381 ccttgtgcca aaaaaaccac tgccctcccc agaccaacct gaggaaagca gtgattctta
    31441 ttttaggagt taggaatgaa tacacgaaat caatttagct gattattttt tgtttttcaa
    31501 gaattcatcg tattgttttt gcatttcgtt caatactttt tcgtaggcac cttcagattt
    31561 caatttttcc atcaattctg gaatcgcttt atctgggtct acagtaccag tgttgatagc
    31621 tgtatcaaat tgttgcattg tgttagcaat agctgagatt tcagatttca cattgtcagt
    31681 attgaagata aatccaagcg ctggagattc tttagcttct gccaattctt tcttagaatt
    31741 ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg atccagttgt taccagtgtt
    31801 ccatccaccc atgtgagtgt ttcctttgta gccatcaaga acgcgaacac ggttttcttt
    31861 accttcaatt ttttcccagt tcttgccttc tggaccgtaa acaagaccgt tcaagagttc
    31921 tggattcgta ttcaagaggt tcaagatttc cattgatttt tctttgttct tagagttgtt
    31981 tgagatgaca aagttagcaa cttgtgttgt ttggtttttc ttgatgaagt tagtaattgg
    32041 tttgatttgg atatctttgt tggcaacacg tgaaagcaag ctgttaccgt agtcagctgg
    32101 tcctactgtt tcttcacgaa cgaaccaagt atcttgttga aggtcaaagg aagtatcgct
    32161 tgttgcgaca tcttttggaa tgtagccagc ttcatagaat ttgtgaagag tcttcaagtg
    32221 ttctttgaaa cgaggcactt cgtaacggtt tacaacttta gtagtatcgc cttcaaggtc
    32281 aataacgaat ggaagcccgt ttgctactgg gtagtcaaag ttatcagatg ggatgaaaac
    32341 tttaccaata gcaaatggta ctacgtctgg agctttttct ttaatttgtt tcaagactgg
    32401 ctcaagagtt tcgtaagaag taacacctga aatatcgata ccatatttgg caaggagagt
    32461 tccgttgaag gcaaagtttt gagatgatgc aacgttggct gcaactggaa cagcgtaaat
    32521 tttaccgttt acagtattac ccttgatgta agctgggtca agtgccttgt aaaggtcttt
    32581 accttctttt ttgtacaatt ctgtcaagtc agcataagca cctttttgag catttacaat
    32641 atagttatct gcaaaggcga tatcatagtt ttcaccagat gatgtgataa ctgacatttt
    32701 cttaccatag tcaccccagc caaggtattg gatatccaat ttagcaccaa ctttttcttc
    32761 gatgattttg ttggcatttg ctaacaattc atccaagtta tctggtttgt caccgatttg
    32821 gtacattttg ataacaggtt tgtcacctga atcagcagct tttttgctgt tacctgtcaa
    32881 gttgccacaa gcagcaagac ctgcagccag agcgactaca ctagcagatg caaaagcata
    32941 ttttttccag tttttcatga taaaaactcc tttttttatt tttaaactta taaacaatgt
    33001 aatgatctta tactcaatga aaatcaaaga gcaaactaga aaactagccg caggctgctc
    33061 aaagcactgt tttgaggttg tagataagac tgacgaagtc agttacatat atctacggca
    33121 aggcgacgtt gacgcggttt gaatttgatt ttcgaagagt attaacttca cacaagggaa
    33181 gttgggaact gagaaatgtt ctttttcaat aagcactatt ctttcacacc accgatagtc
    33241 aaacctttta caaagtagcg ttggaagaat ggatataaaa tcgcgattgg aagggttgca
    33301 accacaacca tggccatacg acctgtttct ttcggtagag caactcccag ttgaccagtc
    33361 aagccgaccg ctttggcaat gtagtccata ttttgttgga tttgcatgag caaatattgc
    33421 aatggataca agttatcact cttgatgtaa agaagggcgt tgaaccagtc attccagaaa
    33481 ccaagagctg tcaaaagcgt gatggttgcg atacctggta gtgacaatgg caaacagatt
    33541 tggaagaaaa tccgggcctc actggcacca tcgatacgag ccgattcgag aatggcttct
    33601 ggaatggtct tcttgaagaa ggaacgcatc aagatgatat tgaatggtga gagaagcatt
    33661 ggaacaatca aggcccaaac agtgtcccca agctgaagta cacgggtcac catgatataa
    33721 cctggtacca aaccagcgtt gaacaacata ctgagaagga cgaagatggt aaaaaatctg
    33781 cgatacttaa aggttgtccg tgaaatagcg taggcatagg ttgttgtgat aaagacattt
    33841 gtcaatgtcc caactacggt tacaaagaca gagatgaaga gggcttgtag gattttatcc
    33901 ttaaactgtg ccaaaaactc aaaaccgtct aagccaaatt gggatgggaa gaagctatag
    33961 ccgtattgga ggaggctttt ctcgtctgtc actgaaataa tgataacgaa tacaaaaggt
    34021 aggatacaag agagggcaat caaacccgaa atgatactga agaagatatc tgctttctta
    34081 ctgaaggagt gaatgccgac attatcaatt ttttcttttt taattttctt ttttgccata
    34141 ttctcctcct ttctagaaca aagctgagtt tggatcgact cgtcttgcaa gcaagtttga
    34201 taggataacc agaatcaaac caacaacgga ttggtaaaga ccggctgctg cagccatacc
    34261 gatatctgct gtctgagtca aaccattaaa gacatatacg tccaaaacgt tggttacatt
    34321 gtaaagctga ccagcattgt gtgggatttg atagaagaga ccgaagtctg cgcggaagat
    34381 atttccgact gcaaggatgg tcaatacagt tacaagcgga gtcaactgag gaatggttac
    34441 gttgcgaata cgttgccact tgctagctcc gtccactgtc gctgcttcgt agtaggttgg
    34501 atcaattccc atgatcgtcg catagtacat gacactgcta tatccaaatc ctttccaaat
    34561 acctaggaaa agtaggagat agggccagat gcccaggtca gcgtagaaat tgacttcttt
    34621 gagaccaaga ctttccaata gatgattgaa caccccttta tcaatattta ggaaggcatc
    34681 tgtaaagaaa ctgatgataa cccaagacaa gaagtaaggg aacaacatag aagtttgaaa
    34741 aatcttcacc attctcttag aacggagctc gctgaggata atggcgatcc ctacagatac
    34801 aactaaacct agaaagataa agccaagatt gtagaggaca gtatttcgtg tgataataaa
    34861 ggcgtctctt gaactaaata agaatctaaa attatcgagt ccgacccatt tactatttat
    34921 gatactatct atgaaaccat tactggtcat gtggtagtct ttgaaggcaa ccacgttccc
    34981 aaatactgga atgtaaaaga atagaatcaa ccagagtgcc cctggcaaaa ccatcaagag
    35041 aaagatccag ttgtctctca atgtttttga aaactttttc ataatttcct ccctttttat
    35101 tttgatatcc atctaaaaat tcttttttag acttttgata acgattacat tattagtata
    35161 ctcctatttg caggttaggt taaactccta attatagaaa aaactccaca aattatgtag
    35221 cagatttaaa actttatcac cactatcaaa caaatgtcct aaatcaattg tttattttat
    35281 ctctattagc ccagtgatgg cgtcactctg ttataagcat ccaacaacgg ggtatactga
    35341 aaaatctcca gactagggaa ctcagcgata gttcctaatc tggagatttt taatatgtta
    35401 ttaggcgttt gctttcaact tagcaataac ctctttaaga ttatcaatca actctgctgc
    35461 agtatgctca gagccttttt catctgccaa gaacaaaact gctttttgaa gttctttttg
    35521 agagttttca aggacatcct tatctactgt ttcaaggttt gagtctttaa gaagtttact
    35581 taattccttg gctaatttct tgagtttgat ttgcagactc atcttctcct gctgtttctt
    35641 tgcccgctgt ttgtcctcca tccttagttg ctgactggct ttccttaatg gactctaggg
    35701 aagcaatggc atctttgact gtttgcaaga tatcacgtaa accttgctct gtcaaactat
    35761 catctgtaag agctttctta gcctctgcca aaaccagacg tgctgaatct gtggtaggat
    35821 tcgatacacc tgtcaatgat ctcaaaagat tttctaaggt ttgagtctgc ttactaatac
    35881 tagactaaaa tcaaaaagca ttacataaca gtgatatgaa atcaactaaa gaagaaatcc
    35941 aaaccatcaa aacactttta aaagactctc gtacagctaa atatcataaa cgccttcaaa
    36001 tcgttctata gtaaaatgaa acaagaacag gacaaatcga tcaggacagt caaatcgatt
    36061 tctaacaatg ttttagaagt agatgtgtac tgttctagtt tcaatccatt atatttcgtc
    36121 tgatgggcaa atcttataaa gagattatag aacttttata gtagtttgaa ataagatgtg
    36181 aacaactcta tcaggaaagt caaattaatt tatagaaata ttttagcagc caaggtgtac
    36241 tgttatagat tcaatacact atacttggtg gtttagctct cctgttttct cttttagctt
    36301 taaccagcca taaatggtat tacgtgagat ttggaaaacg tgtgatgctt ctgttatact
    36361 acctgttcgc tcacaataag agagaacttt tttacgaaaa tctattgaat atgccataaa
    36421 aagattatac cacattgtgt actatttttg gttcacttta ctatatcact ttggactata
    36481 tcggtctagt tatgtctaaa cgattatagc tggatactaa gagaaatcaa aaaagcacta
    36541 agggagaacg tcccaaagtg cttaatatca aggcctcaaa gcctatctta tttaataaaa
    36601 tattacaaca ttttgttgta gaattcaacg acaagtgctt cgttgatttc tgggttgatt
    36661 tcgtcgcgtt ctggcaagcg agtcaatgaa ccttccaact tttcagcgtc gaatgatacg
    36721 aatgctggac gtccaagagt agcttctact gcttcaagga ttgctggaac tttcaatgat
    36781 ttttcacgaa ctgagatcac ttgacctgga gttacgcggt atgatgggat atcaacgcgt
    36841 ttcccgtcaa caaggatgtg accgtggttt acgaattgac gagcttgacg acgagtagtc
    36901 gcgagaccaa gacggtaaac aacattatcc aaacgacgtt ccaaaagaag cataaagttg
    36961 aaacctagga ttccaccttt gatttttgta gcttgtacga acaagttacg gaattgtttt
    37021 tcacctacac cgtaagtgaa acgaagtttt tgtttttcag ccaattgcaa accatattct
    37081 gacaatttag aacggttgtt tggtccgtgt tgtcctggta catagttacg acgtgccaat
    37141 tctttacctg tacctgtaag tgaaaggcca aggcgacgag cttgtttcca agatggtcct
    37201 gtataacgtg acatatgaat gtcctcctga tataaataat atttcggtgg aaatagtcac
    37261 ttagaaagcc ctgattcgtg cagatgccct tcgcctaaac agccaaggtt acttgtcata
    37321 agacacctgt tgacgagctt catgctttcc tgctgatatt tcacactaaa ttcattttac
    37381 catgaatagc tggatttgta aaggggtttt aggctttatt ttcactacca gagaggttga
    37441 gaacgagggt aaaggtactt cctagaccgt actggctgct gactgtgatt tccccaccca
    37501 attgatgggc caattcacgc gcaatcgcaa gtcctaatcc atgaccacct gtcttcatgt
    37561 tacgcgaagt ttcgacacga taaaggcgtt tgaaaatatt ttccaaatcc tctggggcaa
    37621 taccctgccc ttcatcggtc acactgattg aaagctggtc cttctccagc ttagccacca
    37681 cttccagctt ggttcctgga gcagaatatt taaaagcgtt atcgaccaga ttcaccaaga
    37741 tacgagaaag cttagcataa tctccctcaa tccgggcaga ctctgggatt acctgcaagt
    37801 ggacatctct tctctcctgc tcaatcaaaa actgaaattc actcatgcac tcaattaaga
    37861 gcttgtccag aaaaatactg tctttactgg tagtttccac ctgatttcta gctgtgttta
    37921 gggtcaaaaa attcaactcc tcaaccagtt tattgagcct ctccgtctgg cgtccaatgg
    37981 ttgctagata atgagcttgc tccgactcct tgataatacc atccaaaatc ccttctaccg
    38041 tcgcttggat cgaagtgata ggagtcttaa tatcatgcga caactgggca atcatcaagc
    38101 ccttttctcg ttcgctttct tccaaggaat caaagcttac ctgcaaatca tgggacatct
    38161 cattaaaagt ttgccctaat tgctgaaatt ctacaggacc ttgaacctcc aaatttgaag
    38221 gaaaatcctt ggccgctacc cgcttggcat gctccttgag tttgcccaac gacgtaaaga
    38281 ctggcaatag gagaaagaga ctaatcccag caccgacaag gctggcaacg atggtcatcc
    38341 caagcaaaaa gtaaatctcg cctttcgcaa tcagcatttt ttgaacagcc caaaaaacaa
    38401 ccaaaatggt taagagggtt gaaataatat atccaaccaa aatataactt tttagtttca
    38461 tgtttgtcct ctcggtttct ctatcttata tcccaacccc caaactgtct taatagtggg
    38521 cgtttggtca ctactatatt ttgccagctc ctgtcgaaga gcatggatat gcacattcaa
    38581 ggtattggtg tcatccacgt agtcttcttt ccagatcttt tcatagaggt ctgtcttgga
    38641 gaaaactcgc tctggattgc tagctaaaat ccacagcaat tcaaatgatt taacagttaa
    38701 atccagcatt tcttctccta tttgaacttc atgactacta tgattcattt ttagattgcc
    38761 aagggaaatc agctctgttt cgcacccacg atgaaggcgg cgcaaaatat tgtggacacg
    38821 caaaactagc tcacgtgggc taaaaggctt agcaataaaa tcatctgctc ccaagctcag
    38881 gccgtaaatc ttgtcctgtt cactggtctt agcagtaata aataggaaag gctgctctgg
    38941 tgataagtat tgaacctcac tgattaaatc ataaccatcc atccgaggca tcatgacatc
    39001 tgtgataatc aaatcaatcg gttttttctt gaacagctct agcgcttcca gtccatcatg
    39061 ggctaccaag acctgataac ctgcctgaat taagtatctc tgatgaatat ctgtgatttc
    39121 tacctcgtcg tcaacgagta aaattgtctt tcccatctgt ctctcctttg ataaaaacag
    39181 tgctatactg ctttaagtat aacactattt ttattgtttt acgatgattt taatctgcta
    39241 ttgaaagaaa gtcctgattg ctgattgagt tgttaaacta atcttagtca gcccactatc
    39301 catcatttat ttccttagct gattagtttt cttctttttt gtttttcaaa cctagaccac
    39361 cgagtaaacc tgcaagcgca agcccaagga aaccaatact tgccattgat gtttgagtct
    39421 caccagtatt tggtagcata gctttatcct ctgacatcat cgtatcagac atcttgttag
    39481 cagaagcagc catgttttca cctgccatcg tgttggtaga acttgtcatg gtgtcagcag
    39541 gcatgctatc tgtagtacct gtagcatgat tgtgattcat cggagtcacg ccagaaccag
    39601 agttagaagg tgataatgaa ccatttgctg tgtctgatgt ttctttaaca tttat
//
LOCUS       DBERYS010000016        37248 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.016, whole genome shotgun sequence.
ACCESSION   DBERYS010000016 DBERYS010000000
VERSION     DBERYS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 37248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.016"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..378
                     /locus_tag="ACL8LA_000937"
     CDS             <1..378
                     /locus_tag="ACL8LA_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812712.1"
                     /translation="IDKLSELSNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITL
                     FFDKNKRNFGDILLSKNITLSFSILLCYLFNPSDIKFAYFSVLTVLLIGITNYARFKN
                     LRLTVVFSVPFLFNAIMSIINSF"
     gene            758..2911
                     /gene="comA"
                     /locus_tag="ACL8LA_000938"
     CDS             758..2911
                     /gene="comA"
                     /locus_tag="ACL8LA_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGS1812713.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKANMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2924..4273
                     /gene="comB"
                     /locus_tag="ACL8LA_000939"
     CDS             2924..4273
                     /gene="comB"
                     /locus_tag="ACL8LA_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGS1812714.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4443..5150
                     /gene="purC"
                     /locus_tag="ACL8LA_000940"
     CDS             4443..5150
                     /gene="purC"
                     /locus_tag="ACL8LA_000940"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGS1812715.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5207..8932
                     /locus_tag="ACL8LA_000941"
     CDS             5207..8932
                     /locus_tag="ACL8LA_000941"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGS1812716.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9025..10467
                     /gene="purF"
                     /locus_tag="ACL8LA_000942"
     CDS             9025..10467
                     /gene="purF"
                     /locus_tag="ACL8LA_000942"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGS1812717.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10504..11526
                     /gene="purM"
                     /locus_tag="ACL8LA_000943"
     CDS             10504..11526
                     /gene="purM"
                     /locus_tag="ACL8LA_000943"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGS1812718.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11523..12068
                     /gene="purN"
                     /locus_tag="ACL8LA_000944"
     CDS             11523..12068
                     /gene="purN"
                     /locus_tag="ACL8LA_000944"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGS1812719.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12152..12661
                     /locus_tag="ACL8LA_000945"
     CDS             12152..12661
                     /locus_tag="ACL8LA_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS1812720.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12686..14233
                     /gene="purH"
                     /locus_tag="ACL8LA_000946"
     CDS             12686..14233
                     /gene="purH"
                     /locus_tag="ACL8LA_000946"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGS1812721.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14355..15617
                     /gene="purD"
                     /locus_tag="ACL8LA_000947"
     CDS             14355..15617
                     /gene="purD"
                     /locus_tag="ACL8LA_000947"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGS1812722.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15874..15990
                     /locus_tag="ACL8LA_000948"
     CDS             15874..15990
                     /locus_tag="ACL8LA_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS1812723.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            16020..16508
                     /gene="purE"
                     /locus_tag="ACL8LA_000949"
     CDS             16020..16508
                     /gene="purE"
                     /locus_tag="ACL8LA_000949"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGS1812724.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16495..17568
                     /gene="purK"
                     /locus_tag="ACL8LA_000950"
     CDS             16495..17568
                     /gene="purK"
                     /locus_tag="ACL8LA_000950"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGS1812725.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            17591..19978
                     /locus_tag="ACL8LA_000951"
     CDS             17591..19978
                     /locus_tag="ACL8LA_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1812726.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIGTDRVSFIDKNNNNIVLSDTERKSIFVFNEHYVDQKV
                     KIAQEGLDTIVILGEQVDIDEELDRLRTQLSESQIESQDYYAEYEEYLDEKNEKSPDF
                     WKKEMTDSLKGVGNWAERDREIKGNRAASPVHNNTFQNFVDLQPILDKNELEVEFNNK
                     KARYFSIRDSAVTINNELSLPDINFDSNELSTLLSEKIEEPELNSRDKYLLTLLSDST
                     KGERHLREVKDFFEDEHQKKCPFCTQSVSEDVKVELTNGITKLLSRAVEEHQSALRGK
                     KIDEINQDFSGYEQIDPILIQSYQNSINALNAKFNEINSIIDKKIDNPYNIVELPNIS
                     FSQELSQAEHDIEKINQAIIKHNSEISGIQKLKVDLLQINNELAFYEIQDAYKKFQEK
                     TNKKAICENNYNNSSKRVKDYEKQISDLEDKKLNIDIAVDEINKSLNYIFFSKNRLAI
                     QNQNGKYYLLSRGKSVVPSRVSVGERNALALCYFFTEIIQQRELADAYSHEYFIVIDD
                     PISSFDMENKVGITSYLKYCLTRFFKGNSNTRVLLMTHDKQTIYDFDIFLKEIMESCK
                     EEEGGQKSKYKKLELVSGKLQEFKTSTHDYTELLEIVFGYALGNSTPTSESFVGNAMR
                     KILEAYGSFNYKKGIAELTTDPLIVEKIDKEYRTYFENLMYRLVLNGESHFKDPVKTL
                     SIDFFDTISDEERKKTARDLLVLLYLLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            20151..21449
                     /gene="purB"
                     /locus_tag="ACL8LA_000952"
     CDS             20151..21449
                     /gene="purB"
                     /locus_tag="ACL8LA_000952"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGS1812727.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(21504..25442)
                     /gene="strH"
                     /locus_tag="ACL8LA_000953"
     CDS             complement(21504..25442)
                     /gene="strH"
                     /locus_tag="ACL8LA_000953"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGS1812728.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(25757..26473)
                     /locus_tag="ACL8LA_000954"
     CDS             complement(25757..26473)
                     /locus_tag="ACL8LA_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1812729.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            26821..28608
                     /locus_tag="ACL8LA_000955"
     CDS             26821..28608
                     /locus_tag="ACL8LA_000955"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGS1812730.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            28605..29081
                     /locus_tag="ACL8LA_000956"
     CDS             28605..29081
                     /locus_tag="ACL8LA_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1812731.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            29109..30014
                     /locus_tag="ACL8LA_000957"
     CDS             29109..30014
                     /locus_tag="ACL8LA_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1812732.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            30001..30816
                     /locus_tag="ACL8LA_000958"
     CDS             30001..30816
                     /locus_tag="ACL8LA_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1812733.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALTLSALGHFALGM"
     gene            30823..31227
                     /locus_tag="ACL8LA_000959"
     CDS             30823..31227
                     /locus_tag="ACL8LA_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1812734.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIGGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            31525..32691
                     /locus_tag="ACL8LA_000960"
     CDS             31525..32691
                     /locus_tag="ACL8LA_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGS1812735.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKKLVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALDCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            32807..33844
                     /locus_tag="ACL8LA_000961"
     CDS             32807..33844
                     /locus_tag="ACL8LA_000961"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGS1812736.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            34051..34905
                     /locus_tag="ACL8LA_000962"
     CDS             34051..34905
                     /locus_tag="ACL8LA_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1812737.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            35055..35312
                     /locus_tag="ACL8LA_000963"
     CDS             35055..35312
                     /locus_tag="ACL8LA_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HGS1812738.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADLVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(35354..35671)
                     /locus_tag="ACL8LA_000964"
     CDS             complement(35354..35671)
                     /locus_tag="ACL8LA_000964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812739.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGQYISIPDTMRFFNFKDFLQLLLTHI
                     KLF"
     gene            complement(35743..36021)
                     /locus_tag="ACL8LA_000965"
     CDS             complement(35743..36021)
                     /locus_tag="ACL8LA_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS1812740.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNA"
     gene            complement(36011..36166)
                     /locus_tag="ACL8LA_000966"
                     /pseudo
     CDS             complement(36011..36166)
                     /locus_tag="ACL8LA_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
     gene            36243..37007
                     /locus_tag="ACL8LA_000967"
     CDS             36243..37007
                     /locus_tag="ACL8LA_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGS1812741.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT        10526 a         7062 c         8578 g        11082 t
ORIGIN      
        1 attgataaat tatctgaact atctaatctt tttacaatta aaatagaaac aatattgaca
       61 gtgatttctg ttgttttcac atttatagca actcttattg aatatggatt ttctctcttt
      121 ctgattacat tattttttga taagaataag aggaactttg gagatattct tttatctaaa
      181 aatataacct taagttttag cattttactt tgttatttat ttaatccatc ggatataaag
      241 tttgcctatt tcagcgtatt gacagtatta ctaattggga taacaaatta tgcgagattt
      301 aaaaatttaa gattgacagt agtgttttct gtaccatttt tatttaatgc tataatgtct
      361 attataaata gtttttagaa atactaaaat ttttttcttt acttaataat gatatgaaat
      421 catttcggtt ttgttaactt gtaattctta tattttctag gtagaacgtt tgttctgcct
      481 atttttttat ccaaaaagtg cagttgggag ggagataggc tcatttggga aggaagtcca
      541 gtttttgttt agtgattggg gtaagatagt tgttatcaga tgagtttata ctcttcgaaa
      601 atcaaattca aaccacgtca acgtcgcctt gccgtatata tgtgactgac ttcgtcagtc
      661 ctatctacaa cctcaaaaca gtgttttgag cagcctgcgg ctagtttcct agtttgctct
      721 ttgattttca ttgagtatta gggaaaagga gatgaatatg aaatttggga aacgtcacta
      781 tcgtccgcaa gtggatcaga tggactgcgg tgtagcttca ttagccatgg tttttggcta
      841 ctatggtagt tattattttt tggctcactt gcgagaattg gctaagacga ccatggatgg
      901 gacgacggct ttgggcttgg tcaaggtggc agaggagatt ggttttgaga cgcgagccat
      961 taaggcgaat atgacgcttt ttgacttgcc ggatttgact tttccttttg ttgcccatgt
     1021 gcttaaggaa gggaaattgc tccactacta tgtggtgact gggcaggata aggatagcat
     1081 tcatattgcc gatccagatc ccggggtgaa gttgactaaa ctgccacgtg agcgttttga
     1141 ggaagaatgg acaggagtga ctctttttat ggcacctagt ccagactata agcctcataa
     1201 ggaacaaaaa aatggtctgc tctcttttat ccctatatta gtgaagcagc gtggcttgat
     1261 tgccaatatc gttttggcaa cactcttggt aaccgtgatt aacattgtgg gttcttatta
     1321 tctgcagtct atcattgata cctatgtgcc agatcagatg cgttcgacac tagggattat
     1381 ttctattggg ctagtcatcg tctacatcct ccagcaaatc ttgtcttacg ctcaggagta
     1441 tctcttgctt gttttggggc aacgcttgtc gattgacgtg attttgtcct atatcaagca
     1501 tgtttttcac ctccctatgt ccttctttgc gacacgcagg acaggggaga tcgtgtctcg
     1561 ttttacagat gctaacagta tcatcgatgc gctggcttcg accatccttt cgattttcct
     1621 agatgtgtca acggttgtca ttatttccct tgttctattt tcacaaaata ccaatctctt
     1681 tttcatgact ttattggcgc ttcctatcta cacagtgatt atctttgcct ttatgaagcc
     1741 gtttgaaaag atgaatcggg ataccatgga agccaatgcg gttctgtctt cttctatcat
     1801 tgaggacatc aacggtattg agactatcaa gtccttgacc agtgaaagtc agcgttacca
     1861 aaaaattgac aaggaatttg tggattatct gaagaaatcc tttacctata gtcgagcaga
     1921 gagtcagcaa aaggctctga aaaaggttgc ccatctcttg cttaatgtcg gcattctctg
     1981 gatgggggct gttctggtca tggatggcaa gatgagtttg gggcagttga ttacctataa
     2041 taccttgctg gtttacttta ccaatccttt ggaaaatatc atcaatctgc aaaccaagct
     2101 tcagacagcg caggttgcca ataaccgtct aaatgaagtg tatctagtag cttctgagtt
     2161 tgaggagaag aaaacagttg aggatttgag cttgatgaag ggagatatga ccttcaagca
     2221 ggttcattac aagtatggct atggtcgaga cgtcttgtcg gatatcaatt taaccgttcc
     2281 ccaagggtct aaggtggctt ttgtggggat ttcagggtca ggtaagacga ctttggccaa
     2341 gatgatggtt aatttttacg acccaagtca aggggagatt agtctgggtg gtgtcaatct
     2401 caatcagatt gataaaaaag ccctgcgcca gtacatcaac tatctgcctc aacagcccta
     2461 tgtctttaac ggaacgattt tggagaatct tcttttggga gccaaggagg ggacgacaca
     2521 ggaagatatc ttacgggcgg tcgaattggc agagattcga gaggatatcg agcgcatgcc
     2581 actgaattat cagacagaat tgacttcgga tggggcaggg atttcaggtg gtcaacgtca
     2641 gagaatcgct ttggcgcgtg ctctcttgac agatgcgccg gtcttgattt tggatgaggc
     2701 gactagcagt ttggatattt tgacagagaa gcggattgtc gataatctca ttgctttgga
     2761 caagaccttg attttcattg ctcaccgctt gactattgct gagcggacag agaaggtggt
     2821 tgtcttggat cagggcaaga ttgtcgaaga aggaaagcat gctgatttgc ttgcacaggg
     2881 aggcttttac gcccatttgg tcaatagcta gaaagaggag aggatgaaac cagaattttt
     2941 agaaagtgcg gagttttata atcgtcgtta ccataatttt tccagtagtg tgattgtacc
     3001 catggccctt ttgctcgtgt ttttacttgg ctttgcaact gttgcagaga aggagatgag
     3061 tttgtccact agagctactg tcgaacccag tcgtatcctt gcaaatatcc agtcaactag
     3121 caacaatcgt attcttgtca atcatttgga agaaaataag ctggttaaga agggggatct
     3181 tttggttcaa taccaagaag gggcagaggg tgtccaagcg gagtcctatg ccagtcagtt
     3241 ggacatgcta aaggatcaaa aaaagcaatt ggagtatctg caaaagagcc tgcaagaagg
     3301 ggagaaccac tttccagagg aggataagtt tggctaccaa gccacctttc gcgactacat
     3361 cagtcaagca ggcagtctta gggctagtac atcgcaacaa aatgagacca tcgcgtccca
     3421 gaatgcagca gctagccaaa cccaagccga aatcggcaac ctcatcagtc aaacagaggc
     3481 taaaattcgc gattaccaga cagctaagtc agctattgaa acaggtactt ccttggccgg
     3541 tcagaatcta gcctactctc tttaccagtc ctacaagtct cagggcgagg aaaatcccca
     3601 aactaaggtt caggcagttg cacaggttga agcacagatt tctcagttag aatctagtct
     3661 tgctacttac cgtgtccagt atgcaggttc aggtacccag caagcctatg cgtcagggtt
     3721 aagcagtcaa ttggaatccc ttaaatccca acacttggca aaggttggtc aggaattgac
     3781 ccttctagcc cagaaaatct tggaggcaga gtcaggtaag aaggtacagg gaaatctttt
     3841 agacaagggg aaaattacgg caagtgagga tggggtgctt catcttaatc ctgagaccag
     3901 tgattctagc atggttgcag aaggtgccct actagcccaa ctttatccat ctttggaaag
     3961 agaagggaaa gccaaactca cagcctatct aagttcaaaa gatgtagcaa gaatcaaggt
     4021 cggtgattct gttcgctata ctacgactca tgatgccggg aatcaacttt ttctagattc
     4081 tactattaca agtattgatg cgacagctac taagactgag aaagggaatt tctttaaaat
     4141 cgaggcggag actaatctaa cttcggagca ggctgaaaaa cttaggtacg gggtggaagg
     4201 ccgtctacag atgattacgg gcaagaaaag ttatctacgt tattatttgg atcaattttt
     4261 gaacaaagag taatgttcgt gtttttagag ttaaataatt tttaaactgt gagaaagatt
     4321 cttcttgcag ttttttcttt acaatttttg aaaaacatct gctatttatt cggttaaatt
     4381 cttgtgtttt ttggtttttt gtggtaaaat gtgctcaagt aatacgaaag gcgaacttta
     4441 aaatgtcaaa acaattgatc tattcgggaa aagctaaaga tatctataca actgaggatg
     4501 aaaatcttat tatttcaact tacaaggacc aggcgactgc tttcaatggt gtcaagaagg
     4561 agcagattgc aggtaaggga gtcttgaata atcagatttc atcttttatt tttgagaaat
     4621 taaatgcggc tggtgtggcg actcactttg tggagaaact ttcagacacg gaacaactca
     4681 ataaaaaggt taagattatt cctttggaag tcgtgctccg caactatact gctggttcct
     4741 tttcaaaacg ttttggtgtg gatgagggaa tcgccttgga gactccgatt gtcgaatttt
     4801 actacaaaaa tgatgatttg gatgatccat ttatcaatga tgagcatgta aaattcctac
     4861 agattgcgga tgaccagcag attgcctact tgaaggaaga aacgcgtcgt atcaatgaac
     4921 tattgaaagt ctggtttgct gagattgggc ttaaattgat tgactttaag ctagagttcg
     4981 gttttgacaa ggatggcaag attatcttgg cagacgaatt ttcaccagat aactgccgct
     5041 tgtgggatgc tgatggcaac cacatggata aggatgtttt ccgtagagga ttgggagaac
     5101 taaccgacgt ttatgagatt gtttgggaaa agttgcaggg tttaaaataa caacctcaag
     5161 gctgtttggg aatattgcaa gagctgaaat aaaggaataa gaattgatgg ataaacgtat
     5221 ttttgttgaa aaaaaggctg attttcaggt caagtcagag agtttggtta gagagctcca
     5281 gcacaacttg ggactgtcaa gcttgaaaag tattcgtatt gtgcaagtat atgatgtatt
     5341 tgacttggtt gaggacttgt ttgcacctgc agagaagcac attttctctg agcaggtaac
     5401 cgaccatgtt ttagatgaag tatctgtgca ggcggatctt gctaactatg ctttctttgc
     5461 cattgaaagt ctaccagggc agtttgacca gcgtgcagct tcgtcacagg aagccttgct
     5521 tttgttggga agttcgagtg acgtgacagt caacacagcc caactttact tggtgaataa
     5581 agatattgat gcgactgagt tggaagctgt caaaaactac ctgctcaatc cagttgattc
     5641 tcgtttcaag gatatcacga tagggattgc caagcaggag ttttcagagt cagacaagac
     5701 cattccaaaa ttgcctttct ttgaaagcta tacagcagaa gactttgctc gctacaaggc
     5761 cgaacaaggg atggccatgg aagtggatga tttgctcttt atccaagact actttaagtc
     5821 aatcgggcgc gtgccaactg agactgaact caaggttttg gacacttact ggtctgacca
     5881 ctgccgtcat acgacttttg agacagagtt gaaacacatc gacttttcag cttctaaatt
     5941 tcaaaagcaa ttgcagtcaa cctatgacaa gtatattgcc atgcgcgagg aattaggtcg
     6001 gtctgaaaaa ccacaaacct tgatggatat ggcgactatt ttcggtcgtt atgagcgtgc
     6061 taatggacga ttggatgata tggaagtctc tgacgaaatc aatgcctgct cagttgaaat
     6121 tgaagtggac gttgatggtg tcaaggaacc ttggctcctc atgtttaaaa acgaaactca
     6181 caaccatcca acagaaattg agccatttgg tggagcggct acctgtatcg gtggagctat
     6241 tcgtgatccg ttgtcaggcc gttcctatgt ttaccaagcc atgcgtattt caggtgctgg
     6301 tgatattaca gcaccgattt cggaaactcg tgctgggaaa ttgccacaac aagtcatttc
     6361 taaaacagca actcatggtt attcttcata tggtaaccag attgggcttg ccacaaccta
     6421 cgttcgtgaa tacttccacc caggctttgt agctaaacgt atggaacttg gtgccgttgt
     6481 tggtgcggct cccaagggca atgttgtccg tgaaaaacct gaagcaggtg atgtgatcat
     6541 ccttctcgga ggtaaaacag gtcgtgatgg tgtcggtggt gcgacgggct cttctaaggt
     6601 tcaaacagtt gagtctgtag agactgctgg tgctgaggtt caaaaaggaa atgccatcga
     6661 agaacgcaag attcagcgcc tcttccgtaa tggcaatgtc actcgtctga tcaagaagtc
     6721 caatgacttt ggggcaggcg gtgtctgtgt ggctatcggt gaattggcag acggtcttga
     6781 aatcgacctc aacaaggtgc ctcttaaata ccagggcttg aatggtacag aaattgccat
     6841 ctctgaatca caagaacgga tggcggtcgt ggttcgtcct gaagatgtgg ataccttcgt
     6901 tgccgaatgt aacaaagaaa atattgatgc tgttgtggtg gcgacagtaa ctgaaaaacc
     6961 aaatcttgtc atgcactgga atggtgagac aatcgttgac ttggagcgtc gtttccttga
     7021 catcaatggt gtgcgcgtgg ttgtcgatgc caaagttgtg gacaaggatg tcaaactccc
     7081 agaagagcgt caaacatctg ctgaaacact ggaatcagat acccttacgg ttctatctga
     7141 cctcaaccat gcaagtcaaa aaggattaca gactatcttt gactgctctg ttggacgctc
     7201 aacggttaat cacccacttg gtggtcgtta ccaactcaca ccaactgagg catctgtgca
     7261 gaaattgcca gttcaacacg gtgtgactca tactgcgtcg gtcattgctc aaggtttcaa
     7321 cccatatgta gctgaatggt ctccatacca cggtgctgct tatgcggtta tcgaagcaac
     7381 tgctcgtttg gtggctgctg gtgccaactg gtccaaggct cgtttctctt accaagaata
     7441 tttcgagcgt atggacaagc aagcagagcg ttttggtcag ccagtagccg ctcttctagg
     7501 ctctattgaa gctcagattc aacttggctt gccatctatc ggtggtaagg actccatgtc
     7561 tggtaccttt gaagaattga ccgttccgcc aaccttggtt gcctttgggg tgacgacggc
     7621 agatagccgt aaggtgctct ctccagaatt taaagctgtt ggggaaaata tctactacat
     7681 cccaggtcaa gccctctctg cagagattga ttttgacttg attaagaaaa attttgctca
     7741 gtttgaagca agccaagctg accataaagt gacatctgca tcagctgtca aatacggtgg
     7801 tgtagttgaa agtttggctc ttgctacctt tggaaactat attggtgcag aggtgacctt
     7861 gcctgaactt aaaacagctt tgacagctca attaggcggc tttgtcttca catctcctga
     7921 agaaattgct ggagtagaga agattggaca aacgaaagca gactttacac tgactgtcaa
     7981 cggtgtgaag ctagatggac acaagcttga cagtgcattt caagggacat tggaagaagt
     8041 ttacccaaca gaatttaccc aagcgaaaga actagaagaa gtaccagctg tggcatcaga
     8101 tgttgtgatt aaagccaaag aaaaggttga aaaacctgtg gtttacatcc cagtctttcc
     8161 aggaaccaac tcagaatatg attcagctaa ggccttcgaa aaagaaggtg cagaggtcaa
     8221 tttggtgcca ttcgtgacct tgaatgaaga agctattgtc aagtcagttg aaactatggt
     8281 tgacaatatc gacaagacta atattctctt ctttgctggt ggattctcgg ctgcggatga
     8341 accagatggt tcagctaagt ttatcgtcaa tatcctgctt aatgaaaaag tgcgtgtggc
     8401 tattgatagc tttatcgccc gtggtggttt gattatcggt atttgtaatg gattccaagc
     8461 cttagtcaaa tcgggtctcc taccctacgg aaactttgaa gatgctaaca atactagccc
     8521 aaccctcttc tacaatgatg ccaaccaaca cgtggccaag atggtggaaa ctcgcattgc
     8581 caataccaac tcaccatggt tggctggtgt gcaagtgggc gatatccacg ctattcctgt
     8641 ttcgcacggt gaagggaagt ttgtcgtgac ggctgaggaa tttgcagagc tccgtgacaa
     8701 tggacaaatt ttcagccaat acgttgactt taacggtaaa ccaagtatgg attctaagta
     8761 caatccgaat ggttctgtcc atgccatcga aggaattacc agcaagaatg gtcaaatcat
     8821 cggtaagatg ggccactcag aacgttatga ggatggtctt ttccaaaata tcccaggcaa
     8881 taaagaccaa cacctgttcg catcagcggt taaacatttc actggaaaat aagacttaca
     8941 gattttctaa tagatagtat cagtaatgta aaagtcatgt aaatctagct cttgatgatt
     9001 acaaatgaaa attaggtata aaaaatgaca tacgaagtaa aatctcttaa tgaagaatgt
     9061 ggtgttttcg gtatttgggg acatccagat gctgctaagt tgacctattt tggactccac
     9121 agtcttcaac accgtggtca ggagggggca ggaatcctct ccaatgatca aggacaactg
     9181 aagcgccatc gtgacatggg gcttttatca gaagttttca gaaatccagc caatttggat
     9241 aaattgacag gagctggtgc gattgggcat gtgcgttatg cgactgctgg cgaagcttct
     9301 gttgataaca tccagccctt cctcttccgt tttcacgata tgcagtttgg cttggctcat
     9361 aatggaaatc tgaccaatgc agcctctctc aagaaagaac tggaacaaag aggagcaatt
     9421 ttcagcgcga cttcggactc tgaaatcttg gctcacctca ttcgtcgcag tcataatcct
     9481 agcctgatgg gcaaaatcaa ggaagcgctc agccttgtca aaggtggttt tgcctatatc
     9541 ttgctgtttg aggacaagtt gattgcggct cttgacccta atggctttcg tccgctttct
     9601 atcgggaaaa tggccaacgg agcggtggtt gtttcctctg aaacctgtgc ttttgaggtc
     9661 attggtgccg agtggattcg tgatttgaag ccaggtgaga ttgtgatcat tgatgacgag
     9721 ggcattcagt atgacagcta tacagatgat actcagttgg cgatttgttc tatggagtat
     9781 atctactttg ctcgccctga ttctaatatc cacggtgtca atgtccatac ggcacgtaag
     9841 agaatgggag cgcaattggc gcgagaattt aagcatgagg cagatattgt agttggtgtg
     9901 cccaattctt ccctaagcgc ggctatggga tttgcggaag aatcaggctt accaaatgaa
     9961 atgggtctga tcaaaaacca atacacccag cgaactttta tccaaccgac tcaagaattg
    10021 cgggagcaag gagtgcggat gaaactgtct gctgtttcgg gtgttgtcaa aggcaaacgt
    10081 gtggtcatgg tggatgattc cattgtacgt ggaacaacct ctcgtcgtat cgttcagctc
    10141 ttgaaagaag cgggtgcgac tgaggttcac gttgccattg gaagtcctgc actagcgtat
    10201 ccatgtttct acgggattga tatccagacc cgtcaggagc tgattgcagc caatcatacg
    10261 gtcgaagaaa ctcgccaaat cattggtgcg gacagtctga cttatctttc aattgatggc
    10321 ttgattgagt cgattggtat cgaaacagat gcgccgaacg gtggtctctg tgtcgcttac
    10381 tttgacggtg actacccaac gcctctctat gactacgaag aagactatcg tagaagtttg
    10441 gaagaaaaga ccagttttta caagtaggcg acagattctc cattaaagaa aaggaaaaaa
    10501 caaatgacaa ataaaaatgc atatgcccag tcgggtgtgg atgttgaagc gggttatgaa
    10561 gttgttgaac ggattaaaaa gcacgtggcc cgtacggagc gtgcaggtgt catgggagct
    10621 cttggtggct ttggtggtat gtttgacctt tccaagactg gggttaaaga acccgtcttg
    10681 atttcaggga ctgacggtgt cggaaccaag ctcatgttgg ctatcaagta cgacaagcac
    10741 gataccatcg ggcaggactg tgtggccatg tgtgtcaacg acatcattgc tgcaggtgcg
    10801 gaacccctct attttctcga ctacgtagcg acagggaaga atgaaccagc taagctagaa
    10861 caagtggttg ctggtgtggc agaaggttgt gtgcaggctg gtgctgccct catcggtggg
    10921 gaaacggctg aaatgccggg catgtacggc gaagacgact atgacttggc tggttttgcg
    10981 gtcggtgtgg ctgaaaaatc tcaaatcatt gacggttcaa aggtggtaga gggagatgtt
    11041 cttctcggac ttgcttcaag tgggattcac tcaaatggtt actctttggt tcgtcgtgtc
    11101 tttgcggatt acacaggtga ggaagtccta ccagaattgg aaggcaagaa acttaaggaa
    11161 gttctacttg agccgactcg tatctatgtc aaggctgtct tgccgctcat caaagaagag
    11221 ttggtcaacg gcattgccca catcacaggt ggtggcttta tcgaaaatgt ccctcgtatg
    11281 tttgcagatg acctagctgc tgaaattgat gaaagcaaag ttccagtgct tccaattttc
    11341 aaagcccttg aaaaatatgg tcagattaaa cacgaagaaa tgtttgaaat cttcaatatg
    11401 ggtgtgggac ttatgttggc ggtcagccct gaaaatgtag agcgtgtaaa agaattgttg
    11461 gatgaagcag tctatgaaat tggtcgcatc gtcaagaaag aaaacgaaag tgtcattatc
    11521 aaatgaaaaa aatagcggtt tttgcctctg gtaatggctc aaattttcag gtgattgccg
    11581 aagaatttcc agtggagttt gtcttttcag accatcgtga tgcctatgtg cttgagcgtg
    11641 caaagcagct cggcgttctg tcctatgctt ttgaactcaa ggagtttgag agcaagacag
    11701 actacgaagc agcccttgtc gaactcttgg aagaacacca gattgacttg gtttgcctag
    11761 caggctacat gaaaatcgtt ggaccaacct tattgtcggc ttatgaaggt cggattgtca
    11821 acattcatcc agcctacttg ccagaatttc caggagctca tgggattgag gatgcttgga
    11881 atgctggcgt gggtcagtct ggtgtgacca ttcactgggt ggattcgggt gtggatacag
    11941 gccaggtcat caaacaggtt cgtgtgccac gactagctga tgataccatt gacagatttg
    12001 aagctcgcat ccatgaagca gagtacaggt tgtatccgga agtagtgaag gctctattta
    12061 cagattgact ttttgatgat tcatatgata tctttgattt taaattggag tcagtgtttg
    12121 ttgaagacgg cttcaaacgg aggtatttgt aatgttagaa tctaaaaaaa caactcgata
    12181 tgtattttat gtctatctga tgttattaac ttggggaatc ttatttaagt ttgaaacaaa
    12241 tcctgaattt atagcatttt tcttagctcc aaggtatatc aattggattc cattttcaga
    12301 accactaata gtcgatggaa aaattgtttt tgctgaaatg ttatttaatc tgatttcctt
    12361 tattccatta ggtgtttgtt tccctttgat aaaaactaat ttatctagtt taagaatagt
    12421 cgggacaggt ttcttgatta gtttattgtt tgagtgctta cagtatattt tagcaatagg
    12481 tataacagat ataacggatt tgactttaaa tacgctaggt gtctgtgtag gcttactgat
    12541 ttatcaaatt tttataagag tgttcaaatc acagactaga aaatggatca atatcttagg
    12601 tatgcttagc cttggttttg cttatcttgt tttactgtta ctgcatttaa ttggtgttta
    12661 actaatgatt aaaaaggaga atataatgac taaacgcgtc ttaatcagcg tctcagacaa
    12721 agcgggcatt gttgaatttg cccaagaact caaaaaactt ggttgggaga ttatctcaac
    12781 aggtggaact aaggttgccc ttgataatgc tggggtggat accattgcta tcgatgatgt
    12841 gactggtttc ccagaaatga tggacggtcg tgtgaagacc ctccacccaa atatccacgg
    12901 agggcttctc gctcgtcgtg acttggatag ccacttggaa gcggctaagg acaacaagat
    12961 tgagctcatt gaccttgtgg tggtcaacct ttacccattt aaggaaacta tccttaaacc
    13021 agatgtgact tatgctgatg cagttgaaaa tatcgatatt ggtgggccgt ctatgcttcg
    13081 ttcagcagcg aaaaatcatg ccagtgttac agttgtggta gatcctgctg actacgctgt
    13141 ggttttggac gaattggcag caaacggcga aacctcttat gaaactcgcc aacgtttagc
    13201 agccaaagta tttcgtcaca cagcggctta tgacgccttg attgcagaat acttcacagc
    13261 tcaagtgggt gaaagcaagc ctgaaaaact cactttgact tatgacctca agcaaccaat
    13321 gcgttacggt gagaatcctc aacaagacgc ggacttttac cagaaagctt tgcctacaga
    13381 ttactctatt gcttcagcca aacagctcaa cgggaaagaa ttgtcattta ataatatccg
    13441 tgatgcagat gctgctatcc gtatcatccg tgacttcaaa gatagtccaa ccgttgtggc
    13501 tctcaaacac atgaatccat gtggaattgg tcaagctgat gacatcgaga ctgcttggga
    13561 ctacgcttat gagtctgacc cagtatctat ctttggtggg attgtcgtcc tcaaccgtga
    13621 ggtggatgct gcgacagctg agaagatgca cggcgttttc ctcgaaatca tcattgcacc
    13681 aagctatacg gatgaagcgc tagctatttt gatcaataaa aagaaaaact tgcgtatcct
    13741 tgccttgcca tttaatgctc aagaggctag cgaagtggaa gcagaataca caggtgtagt
    13801 cggtggactt ctcgtgcaaa atcaagacgt ggtcaaggaa agcccagctg actggcaagt
    13861 ggtgaccaaa cgtcagccaa ctgagacaga agcgactgct cttgagttcg cttggaaggc
    13921 tatcaagtac gtcaaatcaa atggtattat cgtgaccaac gaccacatga cacttggtgt
    13981 tggtccaggt caaaccaacc gtgtggcttc tgttcgcctt gccattgacc aagccaaaga
    14041 tcgtctgaac ggggcggtcc ttgcttcaga tgccttcttc ccatttgcgg ataacgtgga
    14101 agaaatcgcc aaagcaggaa ttaaggccat catccagccc ggtggctctg tccgtgacca
    14161 agaattcatc gaagcagcgg ataaatacgg cttgactatg gtctttacag gtgtgagaca
    14221 ttttagacat taagaagata aaagggaaga aaacagtttc tttccttttt tggcttaaaa
    14281 tgctaactga aacaagatta aaacgaactt ttttgatata atgttggtaa ataattcgca
    14341 aaagaggttg aggaatgaaa ctgcttgttg tcggttctgg tggtcgtgag catgcgattg
    14401 ctaaaaagtt acttgaatca aaagacgtgg aaaaagtctt tgtagctcct gggaatgacg
    14461 gtatgactct ggatggtctg gaattggtaa atatatctat ttccgaacat tataaattga
    14521 ttgacttcgc aaaggccaac gatattgctt ggacctttat cggtccagat gacgcccttg
    14581 cggctggtat cgtggatgat ttccatgcag ctggtctcaa agcctttggt ccgacaagat
    14641 tggcagcgga gctggagtgg tccaaggatt tcgccaagga aatcatggtc aaatatggtg
    14701 tttcgacagc agcctatggc acattttcag atttcgagga agcaaaggcc tatatcgaaa
    14761 agcatggtgc gcctatcgta gtcaaggcgg atggcttggc gcttgggaag ggtgtcgtcg
    14821 ttgctgagac ggttgagcaa gcggtcgaag ccgctcatga gatgcttttg gacaataaat
    14881 ttggtgactc aggtgcgcgc gtggttattg aggaattcct tgaaggagag gaattttcac
    14941 tctttgcctt tgtcaatggt gataagttct acatcatgcc aacggctcag gaccacaaac
    15001 gtgcctacga tggcgacaaa gggcctaaca cgggtggtat gggtgcctat gcgccagttc
    15061 cacacttacc acagagtgta gttgatacag cggttgacac cattgtcaag ccagttctag
    15121 aagggatgat taaagaaggt cgcccttatc tgggagttct ttacgcaggg cttatcctga
    15181 cagctgatgg accgaaagtc attgagttca acgctcggtt cggagatcca gaaactcaga
    15241 ttatcttgcc tcgcttgacc tctgactttg ctcaaaatat cacagatatc ctggatagca
    15301 aggagccaaa tatcatgtgg acggacaagg gtgtgactct gggtgtggtt gtcgcatcca
    15361 agggctaccc gctagactat gaaaggggcg ttgagttgcc agccaagaca gaaggcgatg
    15421 tcatcaccta ctatgcaggg gctaagtttg cggaaaatag cagagcactg ctctcaaacg
    15481 gcggacgagt ttatatgctc gttaccacag cagataccgt caaagaagcc caagccagca
    15541 tctaccaaga actatcccaa caaaaaatag aaggactctt ctaccgaaca gatatcggaa
    15601 gcaaggcaat taagtaaaga tataagaata acgcgccgta gtcgccaaac acgataatgg
    15661 tcgtcgtggt gaaaagacca gaacagtgaa tgttctggtc agggggaaac ttggagaccg
    15721 taggctcaaa gtttaggaat gaaaccgaag gtttgcttcc gcctccatca cctaagacca
    15781 ttatcaaaaa gaaaaataaa aattcacaaa atacgttaat gatcgtatgg tttgcgagcg
    15841 ttagcgagct aatatagaac aatcaccgcc gttgtgaaag aacgattgga tgataatcca
    15901 atcgttcagg gaaattggaa gaccttgggt ttccaattta ggcatgagac acctttggtg
    15961 gctgctgccg tccctcacaa gctaaggtga ttgttgaaaa agaggaaaaa ggagaagaaa
    16021 tgaaaccagt aatttccatc atcatgggct caaaatccga ctgggcaacc atgcaaaaaa
    16081 cagcagaagt cctagaccgc ttcggtgtag cctacgaaaa gaaagttgtt tccgcacacc
    16141 gtacaccaga cctcatgttc aaacatgcag aagaagcccg tagtcgtggc atcaagatca
    16201 tcatcgcagg tgctggtggc gcagcgcatt tgccaggcat ggtagctgcc aaaacaaccc
    16261 ttccagtcat tggtgtgcca gtcaagtctc gtgctcttag tggagtggat tcactctatt
    16321 ctatcgttca gatgccgggt ggggtgcctg ttgcgaccat ggctatcggt gaagctggag
    16381 cgactaacgc agctctcttt gccctccgtc tcctctctgt agaagataag tccattgcgg
    16441 atgcacttgc caactttgct gaagaacaag gaaaaatcgc agaggagtcg tcaaatgagc
    16501 tcatctaaaa caatcggaat tatcggtggc ggtcaactgg gtcagatgat ggccatttct
    16561 gctatctaca tgggccacaa ggttatcgcg ctggatcctg cggcggattg cccggcctct
    16621 cgcgtggcgg aaatcattgt ggcaccttat aacgatgtgg acgccctccg tcagttggca
    16681 gaccgttgcg atgtcctcac ttatgagttt gaaaatgtcg acgctgacgg tttggatgcc
    16741 gttatcaagg atggacaact ccctcaagga acagatctgc tccgcatttc gcaaaatcgt
    16801 atttttgaaa aggacttttt gtcaaacaag gctcaagtca ctgtggcacc ctacaaggtc
    16861 gtgacttcta gcctagactt ggcagatatc gacttgtcga aaaactatgt cctcaagact
    16921 gcgactggtg gctacgatgg tcatggacaa aaggttattc gttcagaagc agacttggaa
    16981 gcagcctatg cgctagcaga ctcagcagac tgcgtcttgg aagaatttgt caactttgac
    17041 cttgagattt ctgtcatcgt gtcaggaaat ggcaaggagg tgacgttttt cccagttcag
    17101 gaaaatatcc accgcaacaa tatcctgtct aagaccatcg taccagcccg catttctgaa
    17161 agtctagtag acaaggctaa agctatggca gtgcgaatcg cagaacaact caacttgtct
    17221 ggaactctct gtgtggaaat gtttgcgact gatgatgacg ttattgtcaa tgaaatcgcc
    17281 ccacgaccac ataactctgg gcactattct attgaagcct gtgatttctc tcagtttgac
    17341 acccatattc tcggtgttct cggagcacca ttaccagtca tcaaactcca tgcgcctgcc
    17401 gtcatgctta acgttctcgg ccagcatgtt gaggccgctg aaaaatatgt cacagaaaat
    17461 tcaagcgccc acctccacat gtatggtaaa atagaagcga agcacaaccg caagatggga
    17521 catgtgactt tgattagtaa tgaaccagat agtgtgggag agttttaggg gagaggggtc
    17581 taaagaaaaa atgtttgaaa aaattaaagg aattaatata aaatcaggta tttttgaaga
    17641 tgaaacaaaa ttagaactct ttgaaggaaa ttttgaggga acaaatccag tacaaaacga
    17701 tagggcaagt ttattgtttg gaaggaatgg ttcagggaaa agtaccattg ctcgaggaat
    17761 aaatcaatta aaaaatggtg agattggaac agatagagta tcatttattg ataaaaataa
    17821 taataatatt gtacttagtg atactgaacg aaaatcgata tttgttttca atgaacacta
    17881 tgttgatcag aaagtcaaaa ttgctcaaga agggttagat acaattgtaa ttttaggtga
    17941 acaggttgat atcgatgagg aactggatag attaagaacg cagttatcag aatcacaaat
    18001 tgaatctcaa gattattatg ctgaatatga agaatattta gatgaaaaaa atgaaaaatc
    18061 tccagatttt tggaaaaagg aaatgacaga ttctttgaaa ggagtaggca actgggccga
    18121 aagagatagg gagataaaag gaaatcgagc tgcatctccc gttcataata atacctttca
    18181 aaattttgtt gatttacaac caatcttaga taaaaatgag ttagaggttg aattcaacaa
    18241 taaaaaagct agatattttt caataagaga ttccgcagtt actataaata atgaactatc
    18301 tttacctgat attaattttg atagcaatga attatcaact ttattgtctg aaaaaatcga
    18361 agaacctgag ttgaattcta gggataagta tttactgaca ttattatctg attccacaaa
    18421 aggtgaacgt catttgagag aagttaaaga tttctttgaa gatgaacatc aaaaaaaatg
    18481 tcctttctgt actcaatcag tatcagaaga tgttaaagtt gaactcacga atggcataac
    18541 taaactacta tcaagagcag tcgaagaaca ccaatccgca ttgagaggta aaaaaattga
    18601 tgagattaat caagattttt ctggttatga acagatagat ccaatattaa ttcagtctta
    18661 tcaaaatagt attaatgctt tgaatgctaa atttaacgaa attaactcaa taatcgataa
    18721 gaagattgat aatccatata atatagttga gttacctaat ataagctttt ctcaagaatt
    18781 aagtcaagca gagcatgata ttgaaaaaat taaccaagca attatcaaac ataattcaga
    18841 aatctcgggt attcaaaagt taaaagtaga tctccttcaa ataaataatg aattagcttt
    18901 ttatgaaatt caagatgcat acaaaaagtt tcaagagaaa acaaataaaa aagcaatttg
    18961 tgaaaataat tataataaca gtagtaaacg agtaaaggat tatgagaagc agatttctga
    19021 tttagaagac aagaaactta atatagatat tgcagttgat gaaattaata aaagtttaaa
    19081 ttacatattt ttttcaaaaa atcggttagc tatacagaat caaaacggaa aatactatct
    19141 gttatcaaga ggtaagtctg tggtccccag tagggtttcc gtaggggaac gaaatgcgct
    19201 ggcattatgt tatttcttta ctgagataat tcaacagaga gagttagcag atgcttatag
    19261 tcatgaatat tttattgtta ttgacgatcc aatttctagt tttgatatgg aaaataaagt
    19321 tggaataacc tcgtatttaa aatattgttt aactagattt tttaagggga atagtaatac
    19381 tagagtttta ctaatgaccc atgataaaca aacaatatat gactttgata tctttttgaa
    19441 agagataatg gaatcctgta aagaagagga aggtggacaa aaatctaaat acaagaagtt
    19501 agaattggtt tctggtaaat tacaagaatt caaaactagt actcacgatt atacagaact
    19561 acttgaaata gtcttcggtt atgcattggg aaatagtaca cctacttcgg aaagttttgt
    19621 tgggaatgca atgagaaaaa ttttagaagc ctatggtagt tttaattata agaagggaat
    19681 tgccgagcta acaacagatc ctttaattgt tgaaaaaatt gataaagaat acagaactta
    19741 ttttgagaat cttatgtata gactggtttt aaacggtgaa agtcatttta aggatccggt
    19801 taaaactttg agtattgatt tctttgatac catttctgat gaagaacgaa aaaaaacagc
    19861 tagagattta ctagttttgc tttatctttt agatgattta catgttttaa aacatttaga
    19921 gggtgtatca aatgcggaaa atagacttga acaatggaag tgtgagattt tagaataaga
    19981 gaatcatacc atttgactta ttgcaatggt tgaagtttgc atgatagctg attagagtaa
    20041 tcaaaggatt attacttcct aattttattt tctacaaacc ctaatttagc atctaataat
    20101 gcaaagtttc ctgagcctca tagataaaaa agatttttaa ggagaaaaac atgatcaacc
    20161 gttactctcg ccctgagatg gcgaatattt ggagtgaaga aaataaatac cgtgcttggc
    20221 ttgaggtgga aatcctctct gacgaggcat gggctgagtt gggggaaatc cctaaggaag
    20281 atgtggcttt gattcgcaag aaggcggact ttgacatcga ccgtattttg gaaattgagc
    20341 aggagacgcg ccacgatgtg gtggctttca cgcgtgcggt ttctgagact cttggtgaag
    20401 agcgcaagtg ggttcactat gggttaactt ctaccgacgt ggtggatact gcctatggtt
    20461 acctctacaa gcaggccaac gacatcatcc gtcgtgacct tgaaaacttc actaatatca
    20521 tcgctgacaa ggccaaggag cacaagttca ccatcatgat ggggcgtact catggtgtgc
    20581 acgctgagcc gacaactttt ggtcttaaat tggcaacttg gtatagcgaa atgaaacgca
    20641 atatcgagcg cttcgagcat gcggctgctg gtgtagaagc tggtaagatt tctggtgcgg
    20701 ttgggaactt tgccaatatc ccaccatttg tagaggagta tgtctgcgat aaacttggca
    20761 tccgtgccca agaaatctct acacaagtcc ttcctcgtga ccttcacgct gagtactttg
    20821 cggttcttgc cagcattgcg acttcaatcg aacgtatggc gactgagatt cgtggtctac
    20881 aaaaatctga gcaacgcgaa gtagaagagt tctttgctaa agggcaaaaa gggtcttcag
    20941 caatgcctca caaacgcaac ccaatcggtt ctgaaaatat gactggtctg gcgcgtgtca
    21001 ttcgtggtca catgattacg gcttatgaaa acgtcgctct ctggcacgaa cgcgatattt
    21061 ctcactcatc agctgagcgt atcatcacac cagatacgac cattttgatt gactacatgc
    21121 tcaaccgttt tggaaatatc gtcaagaact tgacagtctt cccagaaaat atgatccgaa
    21181 acatgaactc gacttttggt cttatcttta gccaacgggc tatgttgaca ttgattgaaa
    21241 aaggcatgac ccgtgagcaa gcctatgact tggtgcaacc aaaaacagcc tactcttggg
    21301 acaaccaagt agactttaaa ccacttcttg aggcagattc agaagtaaca tcacgtctca
    21361 cacaagaaga aatcaatgaa atcttcaacc caatttatta caccaaacga gtggatgata
    21421 tctttgaacg tcttggacta ggtgattaat taaaaaataa acagcgagct tcaatctcgc
    21481 tgtttatttt ttatcgaaaa gacttagtct tcttttcttt tagtgagtcc ataggctgct
    21541 agtgtggaca tgagtcctgc gactactagt cctgcagaat cgtgagttcc tgtttcagga
    21601 agttttttct ctgttaccac aggagctgga tcttgaggaa gagctttgct ttcctcagca
    21661 ggagcagttg atggagctgg ttggcttggg atttctagtc ttggtttttc ttcagcaata
    21721 gcggcttgtc cgttttcatc gcctacatgt gttaccatag ttccgacttc gactatttga
    21781 gtaacggctt cctgtgctac gacactattt acaagtgttt tcacttcctt accatcggca
    21841 gaagtgctca cagagtagaa gttgctacga tgtccattga cgcccttagt aatgacttgt
    21901 gtttttcctt tgagtaagag tggattttca cgagtcactg tggtaaatgg aatttcttct
    21961 ttttggatat ccagtctagg ttttacctca gtagttggtg caagaccact ttcatcaccc
    22021 ttgtgagtta caggagcgcc aacttcaacc acttggttta taacttcttt ggttacctgg
    22081 ctatcaagga ctgtttctgt tgtttttcca ttttcagtga gtacagagat gtaatgagtt
    22141 cgttcacctt tgactcctgc tgtgataata ttttcctgac cagctgggag gttaggattt
    22201 tctttcttga taacttcaaa tggaatttct tcagttcttg tgatgagttc tggtctggtt
    22261 tcaacattgg cagccacttc attttcatcc aggcttcctg aatgagttgc agctggtttg
    22321 aggccttgaa gagcggcttt taggttggct acaagtgtgt caagctcagc ttgtttatta
    22381 cggttgaggt tgtaatttag agctgtttta gctgcgtcaa gggcctcaag actttcttta
    22441 ctatatcctt ctaagtttgt aggaatttta gctaattctt cgcggagagc attataatta
    22501 gcacggaagt agtctttgtt gtggtctgca aaggcagtca tgagttcaaa gatttcctct
    22561 tccttgtact cagcgcttgg tctatctgcc cagattgaaa gcatacttcc gactgttgga
    22621 agatctactt caggatattt ggtagaagct agttgattga atggaacttt accagaattt
    22681 tcaactgctt ttgaaagagg gtaagcttca tcttgtttgt gattgcctat aacataatac
    22741 cagtcaccgt tggtattcaa gaatttatag cctttgcttg ctaggtattg aggtgatgcg
    22801 aggttatatc cccaccagcc tttagaccag taagaaatca agacatcttt gtcaaactga
    22861 acatcgtcct tgtcttcata gtagaagcca tcgttgaagg ccattggttg aagccctctt
    22921 tctttggcca tagctgcgag ggtgttggca tattcggcaa atttgccata gagttgatac
    22981 cacttgaggt agtaccagcc ttgggcacta gtcgcatcgt tggcgtattc gtcagtacca
    23041 aagttgaaaa tctttgtttt acctgcaaag aagtccatgt atttaccgat gagggctttt
    23101 acaaagttca tcgcttcttc gtttttcaag tccatagttg tttttgaaac tttatcaaag
    23161 tgggcttgag gatttttaat acctaatttt tccatggcaa ccagcatagc atccatgtga
    23221 cctggactgt taatagctgg gatgagacca atgtccttag atttagcgta ttcaattagc
    23281 tctgtcactt ctgcctgtgt tagtgcagta ccgtttggat cgtcgtagta agctttagtt
    23341 ccttcgataa tagctttttt aacgtcatca ctagcatagg tttttccgtt ggcagtaatg
    23401 gtcatatcat tgagtagaaa gcgaagtccg tcatttccta gaaggagatg gacatcagaa
    23461 tatccgagct cactggcctt gtctacgatg cgtttgagct ggtccagagt aaagtatttg
    23521 cgtccagcat cgattgagat taccttgttt ttggcaagtt tttcaacctc acgtttagct
    23581 tcttcttctt tttgagcttc aggcgtgagg gtcaagttgt tgacagtttc ttgaagttta
    23641 gcaatggctt gatcaatcgt atcttgttgg gcacggctaa ggttgctatc gagagagcga
    23701 atagcttttt cagcttcttt tacggccgtg acgctttctg cagtataacg gttcaggtct
    23761 tttggtacct cgttaagtgc ttgctctgca gattcataat cagctgcgaa gtattcagcg
    23821 ttggcatttg caaaatgacg catgagtttg aagaggcgtg atggtgaata acgtgcagat
    23881 ggagtgtcag cccaagcagc taccatacca ccgatgattg ggatatcagc tccttctgtt
    23941 tttggtacag aagtgattgg tgtgttttta ataccattga gcccctgatc gagattgtac
    24001 cagccttggc catcagcgtt tcgtccaaga acgtagtacc aagcatcatt ggtattaagg
    24061 atttggtgac ctttttcagc tagtagttta gaagaagcga catcgtagcc tccccaacca
    24121 ccagtccaca tagaaacgat gatgtctttg tcaaaactac caaagcttgt gtcgctattg
    24181 tagtagatac cgtcgttaaa ggccattggt ttgagaccgt gcgattttac aatacgagcg
    24241 aggtcattgg cgtaggcaat aaatttttca tagcctttta cagggtagcc ttcgtttgga
    24301 tagtatttat cagcttgaag cacactccaa cctttagcat ctgtcgcatc attggcatat
    24361 tcatcaagtc cgatgttgaa gatttcagtc tttttcgcga aataagcagc atacttgtcg
    24421 ataagggctt ttgtaaaagc gacagcttgt tcgttgtcaa gatcgacagt acgggctgat
    24481 ttcttcccaa aatagctaaa gttagggttt tggattccca attctttcat ggcattgaga
    24541 atcgcatcca tgtgtccagg actatttact gtcggaatga gaccgatacc tttatctttg
    24601 gcatagttaa tcagatctgt catttgactt tctgttaagt gattgccgtt tggatcgttg
    24661 taataatcat ttgtaccttt ttcaatggcg cgtttgacat cgtcactggc ataggtcttg
    24721 ccgttagctg tgatgctcat atcgtccaac atgaaacgga gtccatcatt tccgactaat
    24781 aggtgtaaat cagtgtagcc ataatgtttc gctttatcga tgatttcctt gagctgttct
    24841 ggtgagaaat atttacgtcc agcatcaata gaaacaattt tctttttcgc tagtttttca
    24901 tttacagttg cagcacgttc ctttcctgtc tctgtttccg gtttgtcagc ctctgctttc
    24961 gcttcatctt ttttagctgg tttatccttg tcagtcttgt ctgtatttga ctctttagaa
    25021 tcaacctctt tcgcttcttc ctttttaggg ctagcttctt cttccttttt attagcagtt
    25081 tctttttcag cagaagttgg agttaccacc tctgctttat cactaggagt tgaactaact
    25141 tcctcttgtg gtttttcttc tgtttttgga agactagcta ccttatcagt agctggagtt
    25201 tctgtttcta cagtttttgg agcttctggt tgaagctctg ctttaggtgt ttcctcagtc
    25261 cgattttcgg atgattgagg ggaatcagaa accgtatgga tggtcggttg gttttctgta
    25321 gtagtaggag taactccatc ggctgcaaca gtctgtgctt ggaaggcaaa tccaattaga
    25381 acagaagctg ctcctacagc gtatttacga atagaaaaac gctgttgttt ttcatgtttc
    25441 attgcaaaac ctcctgattg cattgttata ttgatagcga ttatataaat caacgccttt
    25501 attttatttc ttatattaac gagagtcaag aggagatgac aaaaaactat aataagtata
    25561 aaaaaatata aaatttaaac ttaagatttc agattggtcg aaaaaataca tatatatatc
    25621 tagtataatt tttggttcta tttctataaa atattccaca aattatagaa tcttccaaaa
    25681 ataggtaagc gctacctttt tggtgtggta taataagcat agaaaaagcc cgagcgatta
    25741 ggctcaggct ttcttcttag tgatcacggt cacatgagat gaatttaatc ttgtagtaat
    25801 cagatcgttt gtaagtttca ctgtattcta aaacttggcc agttgattcg agtttggtga
    25861 ttttagtttg taggacagta gggaattgtt catcgactcc gaggactgaa gccgcatgtt
    25921 ctggagttgg aaagactatt tcgttgattt cttcaaagtg ttcatcattc atgtgaatgt
    25981 ggtagtctaa cttgaaacga ttatagatag aactatagta ttcaaggttt ggataatttg
    26041 cgttgatata ttgttctggg atgtaggatg tatggtagat ataaacgaca ccgtttgatt
    26101 cgcggatacg ttcaatcttg tagtagaatt gatcgccgcg tagacccaat ttttccaagt
    26161 aaacaagctt gtttccgcgt tcaattgaaa gaacagttac cttatcatct ttagcattga
    26221 agagttcaat atctgaaaac tctacaagct tgtgtttgcg tgcacgtgaa acgaaggttc
    26281 cttttccttg ttggcggaca atatagccat ctttggcaag gtcgtttaag gcgcgaacaa
    26341 ctgtgataga gctgacatcg tacattgaaa tgagttctgc ttcagtgtaa aatttatctc
    26401 cactgctaaa ctgcccagag atgattttat tttttaattc gtcttttatg tattgatatt
    26461 tgggaattgc catattttta cctctatttt ccttctccat agaaaatttt accataaaag
    26521 cgaaaaaaat agtagtcttt tgaataaaaa attagaataa tagtttgaaa aattaagcga
    26581 tatagggatt gctcatattt tatcaatgca ttctatacaa aattatttta ggtcctatat
    26641 tccgaattta aagagcgttt gaaatgggtc taaaaatgtt tgaaactaat tgaatgtgtg
    26701 tataatagta aaaatgcagt aaatatcccg acaatttcta ttgacaattc aaacagattg
    26761 gtttataatt aatataacaa caaatgaaag cgcaaacttt cgcggtcgga aggtagtttt
    26821 atgacacgat ttgagatacg agatgatttc tatctcgatg gaaaatcatt taagatttta
    26881 tctggtgcca ttcattattt taggattcct ccagaggatt ggtatcattc gctctataac
    26941 ttgaaggctc ttggttttaa tacggtagag acttatgttg cttggaattt acacgagcct
    27001 cgtgaaggtg agtttcattt tgaaggtgat ctggatttag agaaatttct ccaaatagcg
    27061 caggatttgg gtctctacgc aattgtgcgt ccgtctccat ttatctgtgc ggaatgggaa
    27121 ttcggtggct taccagcttg gctcttgacc aagaacatgc gaattcgctc atccgaccca
    27181 gcatatatcg aggcagttgg tcgctactat gatcagttat tgccaagact ggtgcctcgt
    27241 ttgttgaaca atggtggcaa tattctcatg atgcaggttg aaaatgagta tggttcttac
    27301 ggagaagata aggcttacct gagagcgatt cgacagctaa tggaagagtg tggcgtaacc
    27361 tgtcccctct ttacatcaga tggtccatgg cgagctactc tgaaagctgg aaccttaatt
    27421 gaagaggacc tctttgtaac aggaaacttt ggttctaagg caccttacaa cttttcgcag
    27481 atgcaggaat tctttgatga acatggtaag aaatggccac tcatgtgtat ggagttctgg
    27541 gatggttggt tcaatcgctg gaaagaaccg attatcacac gggatcctaa ggaattggca
    27601 gatgcagttc gagaggtttt ggaacaaggc tctatcaatc tttacatgtt ccacggtggt
    27661 acaaactttg gtttcatgaa tggttgctca gctcgaggaa ctttggacct gccacaagtt
    27721 acatcttatg attacgatgc ccttctggat gaagaaggaa atccaactgc taaatatctt
    27781 gcagtcaaga agatgatggc aacacatttt tcagagtatc cgcagttgga accactctac
    27841 aaagagagta tggagttgga tgctattcca ctagttgaaa aagtttcttt gtttgaaacc
    27901 ttagatagct tgtcaagtcc tgtagaaagt ctctatcctc aaaagatgga ggagctggga
    27961 caaagttatg gctacctact ttatcgaaca gaaacaaact gggatgcaga agaagaaaga
    28021 cttcgtatca ttgatggtcg agatagggcc cagctgtatg tcgatggtca gtgggttaaa
    28081 actcaatatc agacagagat tggggaagat attttttatc aaggtaaaaa gaaagggcta
    28141 tctaggttag atatcttgat agaaaatatg gggcgtgtca actatgggca taagttctta
    28201 gcggatacgc aacgtaaggg aattcggaca ggggtctgta aggatctgca tttcttacta
    28261 aactggaaac actatccact cccactagac aatcctgaga aaattgattt ttcaaaagga
    28321 tggactcaag gacaaccagc cttttacgct tatgacttta cagtcgaaga gccaaaagat
    28381 acttacctag acttgtctga gtttggtaag ggagttgcct ttgtcaatgg gcagaatcta
    28441 ggacgttttt ggaacgttgg cccaactctc tcactttata tccctcatag ctatctcaag
    28501 gaaggtgcca accgtatcat tatctttgaa acagaaggtc aatataaaga agagattcat
    28561 ttaactcgta aacctacact aaaacatata aagggggaaa acttatgaca attgtaggat
    28621 gccgtattga tggacgtttg atccacggac aagtagccaa tctttgggct ggaaaactaa
    28681 atgtttcacg cattatggtt gtagacgacg aagttgtcaa caacgatatt gaaaagagtg
    28741 gtttgaaact tgcgacacca ccaggtgtga aattgagtat tttgccagtt gagaaagctg
    28801 cagccaatat tcttgctggt aaatacgata gccaacgtct ctttatcgtg gctcgtaaac
    28861 cagaccgctt ccttggtttg gtagaagcag gtgtaccact tgaaaccctt aatgttggga
    28921 atatgtctca aacaccagaa actcgttcta ttacacgttc tatcaacgta gtagacaagg
    28981 atgtggaaga cttccacaaa ctggcagaaa aaggtgttaa acttactgct cagatggttc
    29041 caaatgatcc aatttcagac tttttgagct tattaaaata ggaaaaaaat ttttaggagg
    29101 tcattgttat gatacaatgg tggcaaattt tacttctcac tttgtactca gcttatcaaa
    29161 tctgtgatga gttgacgatc gtttcatctg caggttcccc tgtatttgct ggtttcatta
    29221 ctggtttaat catgggagat gtgactactg gtttacttat cggtggtaac ttgcaactgt
    29281 tcgttcttgg ggttggtacc ttcggtggtg cttctcgtat cgacgcaact tctggtgcgg
    29341 ttcttgcgac agccttctct gtttcacaag gaattgatgc accgcttgcc attactacaa
    29401 tcgctgtacc agtagcagct ctcttgactt acttcgacgt tcttggtcgt atgactacta
    29461 ccttcttcgc tcaccgtgtg gatgctgcaa tcgaacgctt tgactataaa ggtattgaac
    29521 gcaactactt gcttggtgcg attccgtggg ctctatctcg tgcccttcca gtcttctttg
    29581 cccttgcttt tggtggtgcc tttgtacaat cagtagtaga cttcgttgaa gcctacaaat
    29641 gggttgcaga tggcttgaca cttgcaggac gtatgcttcc aggtcttgga tttgcaatct
    29701 tgcttcgtta ccttccagtt aaacgtaacc ttcactacct tgctatggga tttggtttga
    29761 cagctatgtt gactgttctt tactcatatg taacaggtct tggtggcgct gttgctggta
    29821 tcgtaggtac tcttcctgct gaagttgctg aaaaaattgg tttcgtgaac aacttcaaag
    29881 gtttgtctat gattggtatt tctatcgtag gtattttcct tgcagtgctt cacttcaaaa
    29941 atagccaaaa agtagctgta gcagcacctt ctacaccatc agaaagtggg gaaatcgaag
    30001 atgacgaatt ctaattacaa acttacaaaa gaagatttta atcaaatcaa caaacgtagc
    30061 ttgtttactt tccaattagg ttggaactac gaacgtatgc aagcttctgg ttacctttac
    30121 atgatcttgc ctcagttgcg taaaatgtat ggtgatggaa ctcctgaatt gaaagaaatg
    30181 atgaaagttc atactcaatt cttcaatact tcaccattct tccataccat tatcgctggt
    30241 tttgaccttg ccatggaaga aaaagatggt gtaggttcaa aagacgctgt taacggtatc
    30301 aagacaggtt tgatgggacc attcgctcct cttggggata caatctttgg ttcacttgta
    30361 cctgctatca tggggtcagt cgcagcaact atggctatcg ctggccaacc ttgggggatc
    30421 ttcctttgga ttgcagttgc agtagcgtat gacatcttcc gttggaaaca gttggaattt
    30481 gcttacaaag aaggggttaa ccttatcaac aacatgcaaa gtaccttgac agctttgatt
    30541 gacgctgcat ctgtacttgg tgtcttcatg atgggtgctc ttgtagcaac agtgattaac
    30601 tttgaaattt cttacaagtt gccaatcggt gaaaagatga ttgatttcca agacatcttg
    30661 aaccaaatct tcccacgttt gcttccagca atctttactg cctttatctt ctggttgctt
    30721 ggtaagaaag gtatgaactc tactaaagct atcggtatta ttatcgtact tgctttgact
    30781 ctttctgccc ttggtcactt tgcacttgga atgtaattcc ttatgactaa atcattaatt
    30841 ttggtgagcc atggtcgctt ctgtgaggag cttagaggta gcacagaaat gattatgggc
    30901 ccacaagaca acatttacac agtagctctt cttccagaag atggcccaga agaatttact
    30961 gctaaatttg aagctgttat tggaggattg gatgatttcc tagtctttgc ggatcttctc
    31021 ggtgggacac cttgtaatgt ggtgagtcgc ttgatcatgg aaggtcgtga tattgacctt
    31081 tacgcaggga tgaatcttcc aatggtgatt gaatttatca atgcgagcct tacaggcgca
    31141 gatgcggact acaagagccg tgctgcagaa agcattgtga aagttaatga cctgttagcg
    31201 ggcttcgatg atgacgaaga tgaataatac tcttcgaaaa tctcttcaaa ctacgtcaac
    31261 gtcgccttgc cgtaggtata tgttactgac ttcgtcagtc ttatccggca acctcaaaac
    31321 ggtgttttga gctgacttcg tcagttctat ctgcaacctc aaagtagtgc tttgagcagc
    31381 ctgcggctag tttcctacag attttagttg gaactcgatt caattcatgt gacaacgtga
    31441 aaatcgttag agcattttat atagaatata catgggaatg gagcttactc ccattcccat
    31501 atttaataga aaaagaggaa ctcaatgcta cattatacaa aagaagactt gctcgaattg
    31561 ggtgcagaaa tcactacgcg tgaaatctac caacaacctg atgtatggag agaagctttt
    31621 gaattttatc aagcaaaacg tgaagaaatt gcagccttcc tacaagaaat cgctgataaa
    31681 catgactata ttaaggttat cttgacaggt gctgggactt ctgcttatgt gggagacacc
    31741 ttgctacctt actttaagga agtctatgac gaacgcaaat ggaatttcaa tgctattgcg
    31801 acaacagata tcgttgccaa tccagcaacc tatttgaaaa aagatgtggc aactgtcctt
    31861 gtgtcttttg ctcgtagtgg gaactcgcct gaaagtgtgg cgactgttga tttggccaaa
    31921 tccttggtgg atgagcttta tcaagtgacg attacttgtg cagcagatgg taaattggct
    31981 cttcaagctc acggtgatga ccgtaatctc ttgctcttgc aaccagctgt ctctaatgat
    32041 gctggatttg ccatgacttc tagctttacg tctatgatgt tgacagctct cttggtcttt
    32101 gatcctacag aatttgctgt taagtctgaa cgttttgaag ttgtatctag tcttgcccgt
    32161 aaagttttag acaaggcaga agatgtcaaa aagctcgttg atttagactt taaccgtgtc
    32221 atctatctag gcgctggtcc tttctttgga cttgctcatg aagctcagct caagattttg
    32281 gaattaactg ctggtcaagt tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt
    32341 ccaaaatctc ttatcaacga caatacagtt gttttggtct ttggtacaac gacagactac
    32401 actcgtaagt acgacttgga cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt
    32461 gttgtgcttt tgagtgatca agcttttggt cttgaaaatg tcaaagaagt ggcccttgat
    32521 tgtggcggtg tcttgaatga tatttaccgt gtcttccctt acatcgttta tgcccaactc
    32581 tttgccctat tgacttcact caaggtagaa aataaaccag atacaccgtc tcctacaggt
    32641 acagtaaacc gtgtagtaca aggtgtcata attcacgaat atcaaaagta agacagtgtt
    32701 tatgaattct tgacaagagg atttgtaaat tatcagataa accatagatt gtcagtacgc
    32761 tttctatggt ttgtttgctt gagagaaata gtaaaaggag aacagaatga aagcatacac
    32821 agagcgtgta tttggaaatg ttgagggcga ggatgtcttg gcctatcgat ttgagacaga
    32881 cggtggctac caacttgagg ttatgactta tggcgcgacc atcttgcgct atgtcgcacc
    32941 tgacaaggct ggaaattttg ccaatgttat cttgggattt gatgactttg atagttatgt
    33001 aggcaatagt cccaagcatg gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc
    33061 gacctttgag ctcaatggta agacctatga ccttgaggtt aataatgcta gcaactgtaa
    33121 tcacagtggt tcaactggtt gggattccag cttgtttgaa gttgaagaag taagcgatca
    33181 tggcttgact ctctacacag agcgtacaga tgggacagga gggttccctg gaaatctcaa
    33241 gatttggatc agttatcact tggaagaaac tggtgcctat gaaatcagct acaaggtaac
    33301 gaccgatcag gatacgctgg tcaatccaac caaccacagc tatttcaact tgtctggtga
    33361 tttcacgcag acgattgacc gtcatgtctt ccaactaaac acagagggca tttactcaat
    33421 cgctcctgac ggtgttcctg ccaaaactcc agaagccaac cgtgatgtgg tcaaacacat
    33481 ctacaatggt gccttgttga aggatatctt tgcagaagaa gatgagcaaa tccagctggc
    33541 atcaggtttg gatcatccat ttgcccttcc tgcaggccat gacaatgctg gattccttta
    33601 tgaccaaaat tcaggtcgct tcctgctttt caagacagaa gctccttgct ttgtggtcta
    33661 cacagcaaac tttgtggatg aaagtgtcat cataggaggt cagccaatgc tacagcacaa
    33721 tgggattgct cttgaagcgc aagctttacc agatgccatt cacagtgacc ttaaaggcca
    33781 agtcattctt aaagctggtc aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa
    33841 ataaaaaagc tggtcaaaaa gtctttaaaa ttaaaataac gcatattgtc aggtgatgaa
    33901 aagtttgata atatgcgttt tattatgaaa tgatttactt tatattatcc tgaaatggtg
    33961 ttatttttga gttatctggc ttgactttct tgatggaaat tatataatag ttgcaacaat
    34021 aataattaca acgaaaaaag aggaccattt atgacctatg aatacaagag tcacatttat
    34081 ttggcagaga cagttttaaa tgtaaaggat ttggccagtc aaacagcctt ttatcagcaa
    34141 gttatcggtt tagaaatctt atctcaaact gagacagagt ccattctagg ccttggtgga
    34201 aaagtcttgg tacagctgat tcaagcacaa gagagcggag aagtgaggga acattatggt
    34261 ctttaccatt tggccattct cttgcccaca cgcaaggctt tggctgatgt cttgaaacac
    34321 ctgacagatt tacagattcc tcttgtcggc ggtgcagacc atggttacag tgaagctatt
    34381 tacttggaag atttggaggg aaatggcatt gaactctatc gagataagcc agtttccaca
    34441 tgggatattc gagaagatgg acgcattatc ggagtgacgg aggctcttgc ggcgcaggac
    34501 atttatgagt tgggggaaag agtagagccc tttatcctgg cagaaggtac gagaatgggg
    34561 catattcatc tttcagtcaa ggatagtcga aagtccagcc agttttatca aaaggtgtta
    34621 gggctagagg ataaattcag tgtgcctagc gctagttgga ttgcggctgg agattaccat
    34681 catcatttag cagttaacga atggggagga aaaggtctgg cttcgcgtaa gcaaggctta
    34741 ccaggtttag cctactatgt catcgaagtc gcacataaaa aagaactgtt aacgattgcc
    34801 caacgagcac aagaagttga cgcaccaatc aaatggatga catcgagcca attggaaatc
    34861 acagactcag atggcatcgt gacccgtatt cgtttagcaa ggtaaaatta ggaattttta
    34921 ataagttata agtattgcta gtttgctttt tgaacggtct ttggtaacag aactgcgtga
    34981 gctggatggg tgagaatttt gacaaagaag ctgattagag atataatgaa gaaaagtcga
    35041 gcaaggaaaa agaaatgaca aattcaaagt atattacttg tttgaaacgt tcagagggcc
    35101 agttgtgtgg gattcaaaag atgattgaag gagatcgtga ctgtgctgat cttgtgacgc
    35161 aactgacagc agtgagatct agtgtagagc gcgtgattga gatgataatt accgaaaatc
    35221 ttactgaatg tatcaatcag ccactagatg attctgaagc tcaaaaggaa cgcttagaaa
    35281 aggctatccg atacttgatt aaacggaaat aaatgaatgt tttgagacga tatgtgcaaa
    35341 ggtctattaa ctattagaat agtttaatgt gagtcaaaag aagttgtaaa aaatctttaa
    35401 aattaaaaaa acgcatagta tcaggtattg aaatgtactg cccccaaaag ttagattttt
    35461 tctgtctaac ttttgggggg cagtacataa aaaccttgat agtatgcgtt ttattgtggg
    35521 gagatttata ctcttcgaaa atctcttcaa accacgtcag cttcaccttg ccgtagatat
    35581 ggttactgac ttcgtcagtt ctatccacaa ccttaaaaca gtgttttgag cagcctgcgg
    35641 ctaacttcct agtttgcact ttaattttca ttgagtatta cttcatcttc tcatgaaatt
    35701 gagtttttgc ccagctcctc ttgattattc tacttgaccg ggctaagcat tcataccacc
    35761 ttctacgttg gtaacggtga ggccttggga gctgagaaat tggcaggtag atgcagagcg
    35821 gactcctcct tgacaaataa catagtattc atggtcaggt ttgagttctt tgtagccttg
    35881 ctctaaggta cttaatggaa gatttttggc acctggtgca tgtcctgctt ggaattcatg
    35941 tgcttcacgg acatcgataa gttctagatt ttcattttga tattttccat aaaagtcagc
    36001 catgctgata ctagtttcca tattctactc cttctgggtt agccattttg tatagtgaat
    36061 aagcgccgtc aaggttttgg acggtaaatc ctgcttgctt gaggataggc tctgcgatat
    36121 agctgcgcaa actactgtgg caactgacga tgtaggcttg tttttctcct cagttcatca
    36181 gcttcctcct tgacactcgg tcagattttg atacaatagt acaaaattag aggaggtggg
    36241 ctatgattca gaaacatgcg attcccattt tagagtttga tgataatcct caggcggtta
    36301 tcatgcccaa tcacgagggg ttggatttgc agttgccaaa gaagtgtgtc tatgcttttt
    36361 taggtgagga gattgaccgc tatgcgaggg aagtaggggc gaactgtgtt ggcgaattcg
    36421 tttctgctac caagacctat ccagtctatg tcatcaacta caagggtgag gaggtctgtc
    36481 tggctcaggc tcctgttggc tccgctccag cagcccagtt tatggattgg ttgattggct
    36541 atggtgtgga gcagattatc tctactggga cctgtggtgt cctagctgat atagaggaaa
    36601 atgcctttct agtccctgtt cgcgctttgc gagatgaggg agccagttac cactatgtgg
    36661 caccttgtcg ttatatggaa atgcagccag aggctattgc tgctattgag gaagttttgg
    36721 aagacagagg gattccttat gaagaagtca tgacctggac gacagacggt ttttaccgag
    36781 aaacggctga aaaggtggct tatcgtaagg aagaaggctg tgctgttgtg gagatggagt
    36841 gttctgctct tgctgcagta gctcaattgc gtggggttct ctggggtgaa ttgttgttca
    36901 cagcagattc tctagcggac ttggaccagt acgacagtcg tgactggggc tcggaagctt
    36961 ttaataaggc gctagaactg agtttagcaa gtgttcacca cctttagttg tactggcaaa
    37021 ggatttgttt tatcataaaa tgtctagctc atacttttca aaaatatgtt taaacgaggt
    37081 caccttcctc ttgtcctagg catgttgagg ttgggaaaaa tctttaaaat cagaaaaacg
    37141 tatcatatca ggtgatgaaa attttgatac tatgcgtttt atgttggtaa gatttagagt
    37201 gagatgaaat gatactcttc gaaaatctct tcaaaccagg tcagcttc
//
LOCUS       DBERYS010000017        35192 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.017, whole genome shotgun sequence.
ACCESSION   DBERYS010000017 DBERYS010000000
VERSION     DBERYS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.017"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(99..449)
                     /gene="rbfA"
                     /locus_tag="ACL8LA_000968"
     CDS             complement(99..449)
                     /gene="rbfA"
                     /locus_tag="ACL8LA_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGS1812742.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(700..3492)
                     /gene="infB"
                     /locus_tag="ACL8LA_000969"
     CDS             complement(700..3492)
                     /gene="infB"
                     /locus_tag="ACL8LA_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGS1812743.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3509..3808)
                     /locus_tag="ACL8LA_000970"
     CDS             complement(3509..3808)
                     /locus_tag="ACL8LA_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGS1812744.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3801..4094)
                     /locus_tag="ACL8LA_000971"
     CDS             complement(3801..4094)
                     /locus_tag="ACL8LA_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGS1812745.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4116..5252)
                     /gene="nusA"
                     /locus_tag="ACL8LA_000972"
     CDS             complement(4116..5252)
                     /gene="nusA"
                     /locus_tag="ACL8LA_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGS1812746.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5296..5775)
                     /gene="rimP"
                     /locus_tag="ACL8LA_000973"
     CDS             complement(5296..5775)
                     /gene="rimP"
                     /locus_tag="ACL8LA_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGS1812747.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(5901..6536)
                     /gene="trmB"
                     /locus_tag="ACL8LA_000974"
     CDS             complement(5901..6536)
                     /gene="trmB"
                     /locus_tag="ACL8LA_000974"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGS1812748.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6533..7327)
                     /gene="ccrZ"
                     /locus_tag="ACL8LA_000975"
     CDS             complement(6533..7327)
                     /gene="ccrZ"
                     /locus_tag="ACL8LA_000975"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGS1812749.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7488..8099)
                     /locus_tag="ACL8LA_000976"
     CDS             complement(7488..8099)
                     /locus_tag="ACL8LA_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1812750.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            complement(8250..8483)
                     /gene="blpZ"
                     /locus_tag="ACL8LA_000977"
     CDS             complement(8250..8483)
                     /gene="blpZ"
                     /locus_tag="ACL8LA_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGS1812751.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(8525..9214)
                     /locus_tag="ACL8LA_000978"
     CDS             complement(8525..9214)
                     /locus_tag="ACL8LA_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1812752.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            complement(9266..9649)
                     /locus_tag="ACL8LA_000979"
     CDS             complement(9266..9649)
                     /locus_tag="ACL8LA_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGS1812753.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(10278..10597)
                     /locus_tag="ACL8LA_000980"
                     /pseudo
     CDS             complement(10278..10597)
                     /locus_tag="ACL8LA_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            complement(11117..11236)
                     /locus_tag="ACL8LA_000981"
     CDS             complement(11117..11236)
                     /locus_tag="ACL8LA_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS1812754.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(11340..11489)
                     /gene="blpO"
                     /locus_tag="ACL8LA_000982"
     CDS             complement(11340..11489)
                     /gene="blpO"
                     /locus_tag="ACL8LA_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HGS1812755.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(11733..11981)
                     /gene="blpK"
                     /locus_tag="ACL8LA_000983"
     CDS             complement(11733..11981)
                     /gene="blpK"
                     /locus_tag="ACL8LA_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HGS1812756.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(12050..12319)
                     /gene="blpJ"
                     /locus_tag="ACL8LA_000984"
     CDS             complement(12050..12319)
                     /gene="blpJ"
                     /locus_tag="ACL8LA_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGS1812757.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(12786..12983)
                     /gene="blpI"
                     /locus_tag="ACL8LA_000985"
     CDS             complement(12786..12983)
                     /gene="blpI"
                     /locus_tag="ACL8LA_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGS1812758.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            13265..13852
                     /locus_tag="ACL8LA_000986"
     CDS             13265..13852
                     /locus_tag="ACL8LA_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS1812759.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            13779..15422
                     /locus_tag="ACL8LA_000987"
     CDS             13779..15422
                     /locus_tag="ACL8LA_000987"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HGS1812760.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            15433..16794
                     /locus_tag="ACL8LA_000988"
     CDS             15433..16794
                     /locus_tag="ACL8LA_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGS1812761.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            16851..16979
                     /gene="blpC"
                     /locus_tag="ACL8LA_000989"
     CDS             16851..16979
                     /gene="blpC"
                     /locus_tag="ACL8LA_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGS1812762.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(17050..18390)
                     /locus_tag="ACL8LA_000990"
     CDS             complement(17050..18390)
                     /locus_tag="ACL8LA_000990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1812763.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(18404..19141)
                     /locus_tag="ACL8LA_000991"
     CDS             complement(18404..19141)
                     /locus_tag="ACL8LA_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1812764.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(19146..19484)
                     /locus_tag="ACL8LA_000992"
     CDS             complement(19146..19484)
                     /locus_tag="ACL8LA_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1812765.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            19760..20089
                     /locus_tag="ACL8LA_000993"
     CDS             19760..20089
                     /locus_tag="ACL8LA_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS1812766.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(20257..21306)
                     /locus_tag="ACL8LA_000994"
     CDS             complement(20257..21306)
                     /locus_tag="ACL8LA_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812767.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(21303..22034)
                     /locus_tag="ACL8LA_000995"
     CDS             complement(21303..22034)
                     /locus_tag="ACL8LA_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812768.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            22102..22512
                     /locus_tag="ACL8LA_000996"
     CDS             22102..22512
                     /locus_tag="ACL8LA_000996"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1812769.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            22522..22809
                     /locus_tag="ACL8LA_000997"
     CDS             22522..22809
                     /locus_tag="ACL8LA_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812770.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            22971..23255
                     /locus_tag="ACL8LA_000998"
     CDS             22971..23255
                     /locus_tag="ACL8LA_000998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812771.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(23567..24703)
                     /gene="dnaJ"
                     /locus_tag="ACL8LA_000999"
     CDS             complement(23567..24703)
                     /gene="dnaJ"
                     /locus_tag="ACL8LA_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGS1812772.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(25105..25461)
                     /locus_tag="ACL8LA_001000"
     CDS             complement(25105..25461)
                     /locus_tag="ACL8LA_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HGS1812773.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVPAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(25463..27286)
                     /gene="dnaK"
                     /locus_tag="ACL8LA_001001"
     CDS             complement(25463..27286)
                     /gene="dnaK"
                     /locus_tag="ACL8LA_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGS1812774.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(27766..28290)
                     /gene="grpE"
                     /locus_tag="ACL8LA_001002"
     CDS             complement(27766..28290)
                     /gene="grpE"
                     /locus_tag="ACL8LA_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGS1812775.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(28343..29377)
                     /gene="hrcA"
                     /locus_tag="ACL8LA_001003"
     CDS             complement(28343..29377)
                     /gene="hrcA"
                     /locus_tag="ACL8LA_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGS1812776.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(29541..30050)
                     /locus_tag="ACL8LA_001004"
     CDS             complement(29541..30050)
                     /locus_tag="ACL8LA_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGS1812777.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            30658..32991
                     /gene="hsdR"
                     /locus_tag="ACL8LA_001005"
     CDS             30658..32991
                     /gene="hsdR"
                     /locus_tag="ACL8LA_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGS1812778.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSTKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            33004..34467
                     /locus_tag="ACL8LA_001006"
     CDS             33004..34467
                     /locus_tag="ACL8LA_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS1812779.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            34467..>35192
                     /locus_tag="ACL8LA_001007"
     CDS             34467..>35192
                     /locus_tag="ACL8LA_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1812780.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRI"
BASE COUNT        10966 a         7368 c         6299 g        10559 t
ORIGIN      
        1 gcgtactatt ctagtttcaa tatattttaa aagaatattt ttccatttca aattcaacct
       61 attttcctcc accaaaaaag aggggcaacc cctcttcttt agttcttatc cagattgcgt
      121 agcatctcgt caatcttgtt tccatactcg atggactcgt ctttgacgaa ggtcaaatct
      181 gggattttgt acaatttcaa attgcgacca agttcacgtt tgatggtacc agttgctttt
      241 tcaagcccga tttgggcttt ttggttatcc gaagcaaggt tactcaaaat ggtgtaataa
      301 accttggcaa cagacaagtc acccagcatc tgaacatctg tgatggtcac accttggaca
      361 cgtggatcac ggactttctt ttgcaaaatc tcattgactt cacgcttgat ttccatgccc
      421 acacgatctg tacggaaatg atttgccatg atattccttt ctctactttg aaccaaaagc
      481 taggccagca gacctagcta ttttaaactt cttgattttc agtttaaatt gaaacgaagt
      541 tcaatttgaa gtcatctgct tctttcgccg tggtgaaagt gatggaactg atttatcagt
      601 cccatcacag gggatttttg agaccctagg ctcaaaaata agcaatgaaa ccatcggttt
      661 gcttgcgtcc ctcaccacct aagaaaggag caaaaaatct tatctcttga tttcttccat
      721 gacatacgcc tcaatcacat catccatctt aatatcattg tagccgtcga tcatcaatcc
      781 accttcacga ccgtttgtca cttctttcac gtcgtctttg tagtgtttca agcttgcgag
      841 ttcgccatca tagataacga caccatcacg gataacacgg actttagagt cacgggcaac
      901 cttaccgttg ataaccataa atccaccgat agttcccact ttagacacct tgaaggtttc
      961 acggataacc gcttcaccaa taactttttc ttcaaattct ggatcaagca tccctttcat
     1021 agcttcttcc atctcttcga taaccttgta gataatgctg tgaagacgga tttccacatc
     1081 gtcagcttct gcttgttgac gagcttgtgg tgtagggcgt acgttgaaac caacgataaa
     1141 ggcatttgaa gcttcggcaa gggtcacgtc tgattcgttg atagcaccga ccgctgagtg
     1201 gacgatagtc actttgacac cttccacgtc aatcttttga agtgaggcag aaagggcttc
     1261 aacagaacct tgtacatcag ccttgatgat aacattaaca gatttgagtt ccccagcttt
     1321 aagggtatca aagaggtttt caaggctaac acgttgggta gcttgacgtt gtttcatgag
     1381 ggcacgtttg gcacgctctt cacctgctgc acgcgcagat ttttcatcct cgtaaacggc
     1441 aaagtggtca cccgccatcg gtgcttcgtt caaacctgtg atagagactg gtgttgatgg
     1501 tccagcaact ttaacacgac gaccaaggtc gttggtcata gcacggacac gaccgaaggt
     1561 atttccgaca acgattggat cttgaacatt caaggtacct tgttgtacaa gaagggttgc
     1621 gaccgcacct tttcctttat ccaagcgcgc ttcgataacc gtaccgattg cacgcactgt
     1681 tgggtctgcc ttgagttctt ggatttcagc tacaagaagg actgtttcca acaattcttc
     1741 gatattttgg ttgaatttag ctgagatttc aacaaattca gaatctccac cccaagcggt
     1801 tgacatcaca ccatgctctg ccaattcacc gataacgcgt tctgggttag cacctggttt
     1861 atcaatcttg ttaatagcta cgatgattgg aacgttagct gcttttgagt ggttgatggc
     1921 ttcaatagtc tgaggcataa ccccgtcatc tgccgctacg accaagatcg taatatcggt
     1981 aacagaagca ccacgcgcac gcattgatgt aaaggccgcg tgtcctggtg tatcaaggaa
     2041 ggtaatcttc ttaccatttt ccacgatttg gtaggcaccg atatgctgag taataccacc
     2101 tgcttcacct gtcgcaacac gtgagttacg aagagtatcc aaaagggttg ttttaccgtg
     2161 gtcaacgtgt cccatgatag taacaactgg tggacgctca accaattcat cttcattgag
     2221 ataaccatct tcgacaaaga aacgttcgat gtcagcatta tccacttcaa ccttttgttt
     2281 ggcttcgata ccgtaatcca ccatgaggag ttcaattgtt tccccatcca aggattggtt
     2341 ttgtgtggcc atgacaccca tcatgaaaag tttcttaaca atttcagctg gttcacgttt
     2401 gatacgtttt gcgatttccg caacggtcat accatctgta tattcaaatt ctgttggcaa
     2461 ttcatggaat ttacgctccg taacaggttt tggagtctga ttacggttgt tcttgttatt
     2521 gccttttttg ttctttttgt tgttattcca gttactattc ttttgatttc tcacttgatt
     2581 ttgactactt cgattctttt gttgttttct aggaccatct tcttcatgat cataatcgtc
     2641 acgatttttg tctggtcgag cttgtttttt acgacgtgta tccactgcag gttctttttt
     2701 ctcagggacg acttcaacca ctgcttgaac ttgctgtgct tgttcagcta acttagccgc
     2761 ttcttcaaag atttcctctg gttccttgcg tttgttagct tgagccaagg cttctttagc
     2821 agcctgatac tgcttgaagc gttcctcact tgaacgagcg tactctgcat tttgctctgc
     2881 ttttagggct gctgcacggg ctttaaagtc aatacgtgga gccgcttgat ttgaacgttt
     2941 atcctcttgc tgacggcgct catttctacg tttttgctga ccttgctgct tcttagcttg
     3001 gtcattaaag cgacgattgt cgcggttgct ttgaccttgt tttccaccat tacggccgtc
     3061 gtttttctga cggtttccgt tttgttgttg gtcacggtta ttgcccttat tttgcttgcg
     3121 tcgttctgcc tgctctttag cacgggcttc acgctcagcc ttgaaattac gactttgcgg
     3181 tttcactgct actttttctg ttttcggagc aactggctca gccggttttg cctcttcctt
     3241 agctacagct ggtttagctg gctcagattt ttcggctttc ttttctgcac ttacttttgg
     3301 tgctgcaggt tttgcttcta ctttcggagc agctgcaggc ttaaagctgg cagcaatttt
     3361 tgcagcgaca gcttcttcca cacttgatga gtggcttttc acatccaagc ccaactcttt
     3421 tgcacgcgct acaacttctt tactttcttt tccaagttct tttgcgattt cgtacaatct
     3481 tttcttagac aaatcatgtc ctcctcttct attccataag agacctcatt ttctttgtaa
     3541 atccagcatc tgttacagcc aaaacctttc tcgatttccc gactgctatg cttaattcca
     3601 gtgttgaaaa cacggttaca atttctactt gataataatg acttttatct tgaatcttct
     3661 tggtcagatt gggtccagca tcatgagcta gaaagaccaa cttggccttg ccgtcttgaa
     3721 tggccttgac caccaattct tcacccgata tgatgcgccc tgctcgctga gcaagcccca
     3781 agagattact tatcttttgc ttattcaagt cccaactctc ttcttttcac tttgtgatcc
     3841 acataagcga tcaactcgtc ataaaagctt tcttccactt ccatgctaaa gctgcggtta
     3901 aagaccttct tctttttcgc ctctagggct tctgcattgt ctagtttgat ataagcgccg
     3961 cggccattgg ccttgcccgt aggatcaata aagacttgtc cttccttgtt cttgacaatg
     4021 cggagcaaat cacgcttatc aatcacttcg ttagacacaa cagacttgcg caaagggatt
     4081 tttcttgttt tcatctttcc ctcctctagc agcttttatt cttctacagt atcgttttct
     4141 acttccaact ctactgaagc agcgtcttcc atggcttcaa attcgctagc agacttgata
     4201 tcgatacggt aaccagtcaa gtgagccgcc aagcgcacgt tttgtccacg acgaccaatg
     4261 gcaagagaaa gcttgttatc tggaacaacc accaaggcac gtttgctgtc gttttcatca
     4321 aagataactt ggtcaacctc agcaggagcg atggcattgt agataaattc agctggatct
     4381 gctacccact cgataacatc gatattttct tcgattggta ccatgcggtc atttttagca
     4441 tcgtaacgag ctgggtggaa tttgctagta atcttcttga tattagcacc accacgtcca
     4501 acgattgtac cgatagcatc cacgtttgga ttgtggctac gaacagcaac cttcgtacgg
     4561 tcacctgctt cacgagccac gctcatgatt tcaacagttc catcataaac ttctggaatt
     4621 tcttgctcca ttaaacgttt gatcatttct ggatgactac ggctaacaaa gacgttcaca
     4681 ccacgagggt tgtcttcaac cttgtaaaca taaacttcga tacgatcatg agaagcaaaa
     4741 acttctccag gaatttggtc ttgttttgac aattgggctt cgatgctacc aaggttgaca
     4801 tagataaagc ggttgtcaaa gcgttctact gtaccagaca tgatttcttg ctcatgttct
     4861 ttgtaagtat tgtaagtgat ggcacgtgtt tgcttgcgca ttttttccat gatggtttgt
     4921 ttggcagact gggctgctac acgaccaaac tcagctggtg cttcttcaaa cttgattttg
     4981 tctccaagtt cataagctga attaatggca agagcatctt tcaagctgat ttccaaacgg
     5041 ctatcaaata cttcatcaac aacttcacgg acagtataaa ctgtaaagtc acctgttttt
     5101 tcgttgaagt caatagctac gctgtctgac tgaccatagc gtctgcgata agcggaacga
     5161 agcgactcta ctactgcgtc gatgatatct tcttttttga ttcccttgtc ttcttccaaa
     5221 atgcggaagg cctctagcat ttctttactc atgttttctt cacttttgaa tcctcaaaag
     5281 ctatcctttc tttttctata atttaactgc taaacgtgct tttgatacta aactgtatgg
     5341 aatttggacg gttttcttac gcgtcttgtc catatattcc atagtcaact cgtcctcttc
     5401 gaaggccaac aaggttcctt caaagacctt ttgcttatcg atggcttggt agagcccgac
     5461 atggatgtat tttccaaccg ctccagcgac ggcatccttg gttttcaaag ggcgttccaa
     5521 acctggactg gtaatttcta ggaaatattg ttctgggaag ggatctggct tgatggtgtc
     5581 taggacagga ctgataattt ctgtcaagtc tgccgtgtcg ttcaaggtaa ttccttcggg
     5641 tttatctaca aaaatactga gaatcatgtc actgccaatc tttccatact cgatatccac
     5701 gagttcaaaa ggagcttcta tgacaggttc tacaacttct ctgactaatt ctacgattgt
     5761 tgcgattgcg tccacctcct cataagcaag aggcgaagat atttccccgc ctcacttttc
     5821 atattcttac tatcagtata gcacgtttgc caacttatgt aaagcaaaag cacttgtaca
     5881 gcctaatttt aggctatctc ttaaaattct gcctcaactc ggtagataac ttgccccttg
     5941 tttgagaatt tttgctcgta ttctgtcatg acattgcctt caaaatcact ggcatgcaaa
     6001 tctaaccaga caccattgag tttcatgcca tattgagaaa agctcactaa actgtactca
     6061 aacaagccac ggttatccgt cttgaaatga atttctccat tttcaggcaa gatacgtttg
     6121 aaggtatcca agaaggtctt gtaggtcaaa cgacgctttt catggcgttt tttcggccat
     6181 ggatctgaaa agttcagata caagcgatca atctcaccgt cttcaaaata gtcagttaag
     6241 tcagaaccat ctacccacaa gagcttgatg ttaggcactc caacttcaag caccttgtcc
     6301 aaagcgtagc tcaaaacaga cttttgaata tcaatcccga tatagttgat gtcagggttt
     6361 tgcttggcca tacctgaaac aaaggcaccc tttccacttc caacttccac atgaatggga
     6421 ttatcattgc caaacaagtc ccgccatttt gccttggctt ccaagggatt gaggaccaca
     6481 tactggggat ttgcctctag taattctgtt gcccctttac gatttctaac tctcatcttc
     6541 tctttccata cttgtctcgg aaatgacgca aaccatgaat ctcccgattg acattttcta
     6601 aatcttggtt catataatac ttggaaatct gactcaaata agacaattga ccataccaat
     6661 acaatttact taataccgtt tgattgtact tgtaaccgta gtaggtcaac cattccttcc
     6721 actgatgttc tgaaatataa tggcagagca tatgggccac atcaaacatg cgatcggtca
     6781 agcgaaccga atcccaatct actaaataaa tcaagccact atctgtctca atccaattac
     6841 tatgtcgtac atctccatgg acaatggtcg catggtcctc tctaaatcct ggaatagtct
     6901 gacgtaaatc agccatcact tcactgataa aatgattttt acgcaaagca tctggagccg
     6961 tttcctgcca agactgtagt aaatctacag gtgtttccat ggcatagccc aaacgactca
     7021 attgtgtcat caacggacgt gagcgatgaa gacgggttaa aatattgacg atttgcttac
     7081 gattcatatc atagggggtc aatatcttgc ctgtcaacca ttcttgagca cacatatcac
     7141 gcccatctgc caaacgacga ctccataata attgtggagc aatttgttct ctagctagac
     7201 caggtaggat tggagaggtg ttcattttta caaagatgcg cttcccgtca ggatagctac
     7261 ccatataagc cttgccactt ttcccaggta tgggagtcag tgttagctca ttatcaccca
     7321 aatccatttc tttcctccgt ataaagtttc cttactattc tactagtttt tggcctattc
     7381 gtcaaccact ctaacccgat tctcaaagaa aagcctctgg attggcttgg gtatcattat
     7441 agcttgaaaa aggtaagcac catcttctgc ttaccttttc ataattttta taaataatat
     7501 aggagtggcc aactgttgta aaacatgtgg actagagtag aaacccatag gttttgactc
     7561 tttttataag caaagcccag caacaaaccc ccaagtagat aaaaaataaa cgaagggaca
     7621 ttagaaggtc catgcatgaa agaaaagaga gaggaagtta gcacaagccc taaccaagaa
     7681 ttgtcaaaaa ccgcaccttg aagaagtcct ctgaaaatga gttcctcctg gatggggcca
     7741 aaaactgagg tagtcaaaat caaagacagg gaaattcctc tgctgacttc cgccaagtgt
     7801 tggacccctg agccagaaga aacagcgaag aaatgaatat aaaccagcgt ctcaagtaca
     7861 cttagaagaa agattgcaat gaaaagcaaa agttgtttct tgcttaacat cccaaaagaa
     7921 atcatttcaa actttctagc ataagcaaca ctcagcgagg ttacaagaag acttacaaga
     7981 atcaatgcag attcattttg aaaaaaaccc ccatggttaa tcgtataagg aactgtaatg
     8041 acaattatta gtactaaaaa tccaaaacac aaggcatgaa atttatcaaa aaactccata
     8101 aaactatcct cctcaccaaa cagacttcct acgcgtcatt ctttagttct agcctttcca
     8161 aaacaaacca ttttatactc ttcgaaaatc aaattcaaac cacgtcaacg tcgccttgcc
     8221 gtactcaagt acagcctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt
     8281 agtaaccaaa atccgaccac atagccagcc cctatgaata tagccattaa agctagcatg
     8341 gaatttagga aattaaaaac caccgcagat acaaaggtta gcacaaaaac attaaaagca
     8401 atggtgtcag aagccaagac tagaatatag ggtgtcaacc gatctaaagt tttggaatct
     8461 aggaaaaata agtgtttata catgataatc tcctctatgg ctgaaaagca agccttttgt
     8521 ttttttaccc caagacccta tgtaaaaaag tcagcaaaaa tggtaatgtt gcgaaaatat
     8581 tattgatcac atgcaccgca taggaaggat aaatgctctt ggtatagcgg gtcaaactag
     8641 caaagatgat gccagatgtt gcaaagatga agatatctaa caaactaggc aggcttgaaa
     8701 aatgaggaag agcaaataaa attgacggaa gaagcaaatc aaggccaaat ctcgaatgct
     8761 taaaaaaggc gtgttgcagt aatcctctat agattagctc ttccataagt ggagtcagaa
     8821 agaacaaagt tatatagata cctagctcag caaagttggt cccactataa ccgatcaata
     8881 cagcccaacc ttcagcagtt gactgaacat gtttagctgt ctgaacgtta aaagagatct
     8941 ggagcactac cactaatact gtcaaaatcg aataccaaag ccattttttt cttggaatgt
     9001 gaaagagata accatgacct gtcttaacca gaatccaaat catgactccg ctaaatagca
     9061 aactcaagaa attttgaatc cagacgaaat tgccaatctg ggaagaaaat tgccaataat
     9121 tttgaataat aagcgtcatc tgagaaagac caaatacgaa aaataagtaa gagaaaactg
     9181 cacttatttt gaatagaagt tgatactttt tcatagaaat cctccctact atgacctcac
     9241 cttgtcaggc tctactgctg taagattaag aagacagttt gtttttttta aggctaacct
     9301 gactactaga taatagatac attaaggcat taaagacaat gaaaatatgt ccatagaata
     9361 aaatcaacct cgcatccaaa ccaagataaa gtttgattat caaaaagatg agcaaaagaa
     9421 tttgaaacca taaggttttt ccaaaaataa atttaaagcg atttcgaata tctacttcct
     9481 tgatttttac cgccacccct ttattagcaa gaaggaaaac tcctgcttca aacaaaccac
     9541 tgtaaagaac aagccaccca atagatacga tagagatttg taaaaatgtc cctaaaagaa
     9601 tatccaacac actactcaag aaaataacaa aaaataatct gtatttcata ttaaatacct
     9661 ccattcattt atttcactaa caatttaata gagccttcta ctcaaatatc ctgtcagaaa
     9721 aggatagaaa gctacttttt ataatacttc aagccccaca tgagcagaag cgtgataaac
     9781 aagcagagaa tacacctata taagcgatta gttgttgata gaattctgtt tctgaaatac
     9841 ctctatacaa acaaatgaca aacataaaat ctgccaagcc gataaacata agttgattgg
     9901 ttctaggact aaccaaatca tcatttactt atatttaaga gtatctcttt tatgttaatg
     9961 tatgttagca ctgaaaagca agacaggcca ataatattta aaatgaacag taacggggtt
    10021 aagtctctaa aaaaattatc tactgacact acaagaaata ctatacatat tatagtcgaa
    10081 actatctttt tcttatccat aattatttac tcctttccta acaaatccaa cttatcaatc
    10141 aagagcgatt tttaacataa tgtagcagca cccgttgcaa ctttgacaag tttagtatat
    10201 cattgttttt taaaattttt catccaaatc ttgaattgtc atcgaaacct cctaaattgc
    10261 aaaaacttca ttagccccta ctttttataa tacttcaaac cccaaatgag tagaagcatg
    10321 atgatcaagc aaagaatagc accaatataa gcgattagtt gttgataaga ttctcctgct
    10381 gtgatacctc tatacaaaca aataatagac ataaagcctg tcaagccgat aaacatgagt
    10441 tgattggttc taggactaac caaatcatca tcttcaaact ctcttaatct catttcccta
    10501 gtaagataaa cagtaaccaa aatagaagtc aagttaataa ctactaaaag aaattggaaa
    10561 actgacgaaa tagaaataga taagtagaaa caagcaaagg caactgaccc aggaacaatc
    10621 tcacaaagaa tatgattctt tttaatcaag aaaatctttc atttcttttc tttcttttta
    10681 attaaagaaa aatagatcat aaccgcaatc atataggcta tggtataaaa tagattatac
    10741 caaatactct ccctaagcgg ataaagaaag atggacatga tcagatacag aacgaaaata
    10801 atcagtattt ttttcttcat aagttttcct cctaaatgtg cgatttatct tagttgagca
    10861 agaacattta cactgctagt atagcactta ttttgacctt ggatcactca aatcataaat
    10921 ggtcatcaaa acctcttgaa ttgtaaaaat taaaaaagca agcatgaaaa acatactttc
    10981 ctctttatat tgtattgata ccaacttgtt tgtagacttt tcatcctgct atcacatatc
    11041 attttgacag gcgaaacaat attaaagaaa ctcccctgta aattaagcta gcaaatacag
    11101 gggagaaatt tattttttag agagtactat ccgtatcctt tttggaagat tttgaaaata
    11161 tttttctaat taagtcatcc atataaggac caaatatacc aactactaaa ccaataataa
    11221 aacttttaaa atccataatt accaccaaca tgttgctgca taggctacac ctccaagtat
    11281 agctccacct gcagcaccag ttacacctat tcctatagca aatggtccca atagaaatgt
    11341 caaaccgttg ttgcacaccc atcaattgcg ccatatgcaa cccctgctgc acaactaatt
    11401 tttcttcccc aatcaatatc tccaccttca acgcaagcaa gcatttcatt atccataaca
    11461 gaaaattgtg acatcatttt tgtattcata tagtatgtca cttttcagtt acggaacaag
    11521 tttaatataa aaattatcaa aaaaacatag gcaataaaga gaaaaattaa tttatcatag
    11581 attagaaata atatgacaaa acaattcaat gatgttaatt caatagtctt ttgtttttta
    11641 tcagagatac ttatggatag ataaataaga taggtttgaa aagcgaagag aataataaag
    11701 aatatagcct tcataaaatt tagctttcat ttttatgatg tagcggtata ggctaaatat
    11761 ccacaaacca ctgctcctcc aattcctcct attgcagcgc cccatggtcc tagaattttc
    11821 ccatatttca ctccacccgc tgcacaacct aaagcagcaa ctacagctgc tcctccaaca
    11881 cctgctttgg caaaatctcc ccaattgcat ccgccacctt caacgcaagc aagcatttca
    11941 gtatccataa cagaaaattg tgacatcatt tttgtatcca tgacaaatac tcctttttta
    12001 aaaaactaaa ataaatcaga atagaatcct cataatttta ctataagtct taccaactta
    12061 gtcccaattt atcaccaacc atacctccta agcatgttaa tccaccccca attgcaccaa
    12121 tgtgtgctcc aacaaatgca ccagcaagtc cagctactcc taaagtggcc aaacctgctc
    12181 cagttccacc agttataatt cccgtagtga ctcctgtaat cagtgcattt tgacaatcag
    12241 tggagctata ccccccttca actttcgcaa gcatttcagt atccataacc tctaactgtg
    12301 acaacatttt tgtattcatg atgaatacct cctttttatt ttcaatttgt taccaaagtc
    12361 ttaaattcaa taaacaaata gatttttttt agtatctttt tgattttctt aaaaaagtat
    12421 atacgtctac tatcttctta aaggtagcag tacctatttt ttagtctaag atttcaataa
    12481 tcttgagtat ctaaaatatc ttaatttcgt tattctcctt gcaataaaaa gttttactat
    12541 actatttatt aacttgcaga aagcaaaaaa tattagtaaa taatagttta tagttaagtt
    12601 ttttattcct accaatccat caactaagta aagcatcaac gattacataa acgattgata
    12661 atataattaa aattttgcta actatcttat tctcatgatt cttagataac tttgatattt
    12721 tgtaagtaag taaataagac agtaaattaa tagcgataat aatactatat ttaagaatca
    12781 taatcttaca aagaggacat aattcctgaa cctacacaaa taagtgttgc tgctccccca
    12841 gttatcggac cagtcgcagc agctaatagt actgctccaa tacaaccacc gattgcagat
    12901 cctaaattgc ctcttcctcc actaactatt tcgagttctt cattatccat aacagaaaat
    12961 tgttccatca tttttgtatt catgacaaat actccttttt tcttttttta tttttgtctt
    13021 gttgtaactt tgataagttt agtatatcat cgttttttaa aatttttcat ccagatcttg
    13081 aattgtcatc gaaacgtctt gaattaggtt ttttatttca agccacctct aaatgtttaa
    13141 aaaaaataat ttctaatcac ttttttacca ttcaggaagt tttaatgact attcaagatt
    13201 tcataaaata tgaacttagt tttatgacat aatagaccta tccactatat gaaaggaatt
    13261 gccaatgact tcttataaac gtacatttgt tcctcaaata gatgcgagag actgtggtgt
    13321 cgctgcctta gcctcgattg ctaaattcta tggttcagat ttttctctag ctcacttgag
    13381 agaacttgca aagaccaata aagaagggac gactgctctt ggcattgtaa aagccgctga
    13441 tgaaatgggc tttgaaacaa gacctgttca agcagataaa acgctctttg acatgagtga
    13501 tgtcccctat ccatttatcg ttcacgttaa caaagaagga aaactccaac attactatgt
    13561 tgtctatcaa acaaagaaag actatctgat tattggtgat cctgaccctt ctgtaaaaat
    13621 cactaaaatg tcaaaagaac gctttttcta tgaatggact ggagtagcta tttttctagc
    13681 taccaaaccc agctatcaac cccataaaga taaaaagaat ggtctactaa gcaagcttcc
    13741 ttcctctgat tttcaaacaa aaatctctca ttgcttacat tgttctctca agcttattgg
    13801 tcactattat caatataggt ggttcttact atctccaagg aatcttggat gaatacattc
    13861 caaatcagat gaaatcaact ttaggaatca tctcagttgg tctggttatc acctatatcc
    13921 tccaacaagt catgagcttc tccagagatt atctcctaac cgttctgagt cagagattaa
    13981 gtattgatgt gattttatcc tatattcgcc atatttttga acttcccatg tctttctttg
    14041 cgacacgtcg tacaggagaa atcatttcac gattcacaga tgctaactct attatagatg
    14101 ccttggcttc taccattctt tctctttttc tggatgtttc tattctgatt cttgtaggag
    14161 gcgtcttact ggcacaaaac cctaatctct tccttctttc tcttatttcc attcctatat
    14221 acatgttcat catcttttct tttatgaaac ctttcgaaaa aatgaaccat gatgtcatgc
    14281 aaagtaattc tatggttagc tctgccatta tcgaagatat caacgggatt gaaactataa
    14341 agtcgctcac gagtgaagaa aatcgctatc aaaatataga cagcgaattt gtagattatt
    14401 tggaaaaatc ctttaagctc agtaaatatt ctattttaca aacgagttta aagcagggaa
    14461 caaaattagt tctgaatatc cttatcctat ggtttggcgc tcaattagtc atgtcgagta
    14521 aaatttctat cggtcagctg attaccttta acacactttt ttcttacttt acaactccta
    14581 tggaaaatat tatcaacctc caaaccaaac tccaatctgc gaaggtcgct aataaccgtt
    14641 tgaacgaagt ctatctagtc gaatctgaat ttcaagttca agaaaaccct gtccattcac
    14701 attttttgat gggcgatatt gaatttgatg acctttctta taagtatggt tttggacgag
    14761 ataccttaac agatattaat ctcacgatta aacaaggaga taaggttagc ctagttggag
    14821 ttagtggttc tggtaaaaca actttagcca aaatgattgt caatttcttt gaaccctaca
    14881 aaggacatat ttctatcaat catcaggata ttaaaaacat tgataaaaaa gtcttgcgcc
    14941 gtcatattaa ttacctacca caacaagcct atatctttaa tggctctatc ttggaaaact
    15001 taactttggg cggtaatcat atgattagcc aagaagatat tctaagagct tgtgaattag
    15061 ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta tcaaactcag ctctctgatg
    15121 gagctggtct atcaggagga caaaagcaac gaatagccct cgctcgtgct cttttaacta
    15181 aagctcctgt tttaatacta gacgaagcta ctagcggtct tgatgtcttg actgagaaaa
    15241 aggttataga taatcttatg tctctaactg ataaaaccat tctctttgta gcccatcgtc
    15301 tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa atcattgaag
    15361 ttggtagtca ccaagagtta atgcaggcgc aaggtttcta ccatcatcta ttcaataaat
    15421 aaggagaatg tcatgaatcc taatcttttt agaagcgtcg agttttatca gagacgttac
    15481 cataactatg cgacagtgtt cattatacct ctttcattac tatttacttt catcttgatt
    15541 ttctcccttg ttgccacaaa agaaattact gttacttccc aaggagaaat cgcccctaca
    15601 agtgtcattg cctccattca gtcaaccagt gataatccta tcctagctaa tcatttagtg
    15661 gcaaatcaag tagttgaaaa aggggactta ctcatcaaat actctgaaac aatggaagaa
    15721 agtcagaaaa ctgccttagc aactcaatta caaagacttg agaagcaaaa agaaggactt
    15781 ggaattttga aacaaagctt agaaaaagcg actgatcttt tttctggcga ggatgaattt
    15841 ggctaccata atacctttat gaattttact aaacaatccc atgatattga actgggtatc
    15901 acaaagacta acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag ttcatcagct
    15961 attgaacaag aaattacaaa agttcaacaa caaattggag aatatcaaga gttgagagat
    16021 gctatcataa ataacagagc acgcttacca actggcaatc cgcaccagtc aattttgaat
    16081 cgttatcttg tagcctcaca aggacaaaca caaggaactg cagaggagcc atttttatct
    16141 caaattaatc aaagtattgc aggtcttgaa tcatctatcg caagcctcaa aattcagcaa
    16201 gctggtatcg gaagtgtagc aacttatgat aacagtttag caaccaaaat tgaagtactc
    16261 cgcactcagt ttttacagac agcctcacag caacaactaa ctgtggagaa tcaattaaca
    16321 gaattaaaag tacaactaga tcaagccaca cagcgtttgg aaaacaatac cttaacctcc
    16381 ccaagtaaag gtatcgttca tctgaacagc gaatttgaag gtaaaaatag aattccaact
    16441 ggtacagaaa ttgctcaaat attccctgtc atcacagata caagagaagt actaatcact
    16501 tactacgtat cttctgacta tctacctcta ctagataaag gacaaactgt aagattaaaa
    16561 ctggagaaga ttggaaatca cggcaccacc atcatcggcc aacttcagac aattgatcaa
    16621 actcctacca gaacagagca aggaaatctc tttaaattaa ccgctcttgc aaaactatct
    16681 aacgaggata gtaaactcat ccaatatggc ttacaaggtc gcgtcactag tgtaactaca
    16741 aagaaaacat attttgatta tttcaaagat aaaattttaa cccattctga ttaattttca
    16801 gataacactc tataactatt tattatctta tcaaaaagga gaatcataac atggataaga
    16861 aacaaaacct aacttcattt caagaactaa caactaccga actcaaccaa attacaggtg
    16921 gaggattgtg ggaagattta ttatataaca ttaatagata tgctcattac atcacataag
    16981 aacttcatca tccaatacaa ctataaaaaa ataagaccga gaaacaagta ctctcggtct
    17041 tatttttcat cattctgtat gtatcacagt aagtacctga cgaaagactt gattttgaca
    17101 ggtggtattt agactggtat taggatggct ttccacaatc ttcatgacgg tatagagacc
    17161 aactcctctc tcctcccctt tagaactggc tccaaaggag aagatttcag aaatatcgat
    17221 gccctcttct ttgatggagt tttcgatgat aaaggtctcc tgtgctccat tttttaaaaa
    17281 ggcgattgaa acatgaggtt gactagcttc cacactggct tcaatagcat tgtcacaaag
    17341 gatagacaca atggttagaa aatcaagtag actcatcccc tcgacctgaa tctcctcagg
    17401 aacttcgaca ttaaagacaa tgttcttatc tctggctttt aaaaatttcc ctgctagaag
    17461 acttttgagg gctttatcac gaatatttac caatctgccc aggtcatatt tattgttctg
    17521 caatttctga ctggaatcct ttaagacgga gccatagacc tcttttatct gctccatatc
    17581 ctcctcttca atgcccagac gtaagctagt caagaggttg gtataatcat gacgaaagct
    17641 ccgtacttcc ttgtaaagct cctctatatg ccgactatag cgttccatat ctctatagcg
    17701 cagggcctgc tcttgttcca atctctcata gagtttttcc ttcaaatagg tatccaattt
    17761 cttgataacc cccataaaaa agagtaggta aaagactagg atgagatggc gaacagtctt
    17821 tgattgaata ctttgttcat attcaaaaaa agacagactt tccatgacta gatagtagcc
    17881 acccattatc cagttaatct gagtcaggga cttttgaaag gctttatcga gaatctcctt
    17941 tctcaagcta gtaaaatcgt agtccaacca tttcaaaaaa gctagagaaa tgaagaaatt
    18001 gaaaattatt atacataacc cagtaaatga gtagccatca tatacttgcc cttgtcccaa
    18061 aaatggaagc acaaaatagg agactcctct ataaaagaga ttcaccaata tcattggaaa
    18121 gagaccataa aagaaaagga gttttttagg aagccctctc aataataaga aagataagcc
    18181 tatgccgtac aagggttcca taaaataaga taggtaaaca tttcctacta tatagctaat
    18241 catcacaaaa acaaaggcca acagtatctt aaaaagaaag gccttaaaaa tcctctcgaa
    18301 agtaagatca attccatcca ccttaaagaa gatgacaatt tctagtccat tagtaacaag
    18361 tgtatacaac aatatccaag caatgttcat aaattctcct agctcagtgt aatttattga
    18421 tggcctcaga cacttccctg accttataac gggcgattag acaacttcca ccattgggaa
    18481 agaaaagcag tttttctttc ttatccaaat gcaccacatt tgcaggattg atgagaaaag
    18541 agcggtggca ctgcaagaga cggggctcct gcttgaaaac ctcctctaaa ctcgctgtaa
    18601 attccagcct gtctgtcttg gtatagagaa taacacgatg ggctctgggc gacgtttcga
    18661 gatagtaaac ctctttaaaa ggatactgaa attgggcaaa ttttgattta aagtaaaagc
    18721 aatcttccgc cagactttta ctatcttgac tattggcata gaggagggct gtctcgatcc
    18781 gagattcaaa ctcctctgct gacaaggcct tatcaatgta gtccaaagca gacacttggt
    18841 agcgaaaaga caggggcata aactccgagt gagtcgtcac aaagacaatc agggcataag
    18901 gatcccgatc ccgaatcttt ctagccactt ccagtccctt catctcttca tttcgaatct
    18961 caatatccaa aaagaatagc tgatgggccc ccttctcatg cacttcagcc agcagttggt
    19021 ccggcttgcc aaatacctca aaagagctag gaatgatatg atgtgctttc aaaagtttct
    19081 caatcgtcgt ttcaattcta gtctgttggg aaaaatcatc ctctaaaaca aatattctca
    19141 tcttcttact ctccctcttt caaccatttt tgacgaattt ctctatagcg acgtctggca
    19201 tacttgacag cgtattgacc ttcttctccg agaaaaagga ttctttcttg aaaatcaatc
    19261 gattttaatt tatcaagatt aaccaaacaa tttcgatgac atctcatcaa ggtttcccca
    19321 cattggttct caagattact taaattttga agcatattaa aactagcttc ttctgtaaca
    19381 atctgtacgg tatgggcttt agttggatgt gtttggatat agtagatatc gtcgatgttt
    19441 actttataga ctgttttctg cgtctgaata atcatatatt tcattggatt ctcctcctaa
    19501 gaaaagaata acagaaaagg tctagcccaa gcacacccaa tcctaaacag tctttgaaaa
    19561 ctcctaaaag gattattttc tacaacatca tgatgagctt acaactattt tatcataaaa
    19621 ttcactcagc gcaattcaag acatttcaat gacaattaaa gatttgaata aaaaaaaaga
    19681 attaaaagtg ataaactaac agtatcaaaa cacagttgcc agaagtaaga ctggcaccca
    19741 gattaaagga ggcattccca tgacagatac agaccccatc aaaagagctc atactttgat
    19801 tactgactta aacaaagcct accaagcatg caaacaggca agcgctgacg acgtccgctt
    19861 tcaggagcaa ttaaactcta ttcttggttt tctagccaag gctgaaacag tggataatcg
    19921 attcttgatt gaactagaaa aattttacca gacttccagt cttctcatgg gactcagcgc
    19981 tcttgatcca gatgctccaa ctcgcgccgc ttggcgggcc tatgaccgct tccactttga
    20041 ccaagtcaag accaagttaa tactcaatga aaatcaaaga gcaaactagg aagctagccg
    20101 caagctgtac ttgagtacgg taagacgacg ctgacgtggt ttgaagagat tttcgaagag
    20161 tataagtctc tacggaccaa ccattatcct gtagtaacaa aaaagaagtt gagacagact
    20221 gaactcaact tcttttttag tgtcgtatca gcaatgttag tcctgcatct gacgttttac
    20281 ctgataaggc aaatacaaga ctagtaaaac caaaccagct cctagtaagg ctagaagggc
    20341 tagtttttct tggaaggtaa tcaacccaac aactaattcc acaagtaaaa caaaactggt
    20401 caatcctcga actacctcct gtaagccttt ttccttttgc cccttgtcca gcggaaagag
    20461 ttgggtcaaa tactggtagt caaaggcatg atagagggcc agcaactgga agagcaagag
    20521 gtagttaaag agaactacca ctgctgtcgc aatccaagct tgctcgataa aaacctgcgc
    20581 cagtacggaa agtaagagaa gacgaagact gagagcaaag aggtcgccat ttcgcagata
    20641 agaacgcaga tagagatttt gccaaatctt tccagacacc ttctgaacag cctttaagat
    20701 aaagtccaga taggcacgac gtttgacact gtttgaaatt ccctttacct gcgtaaagag
    20761 ggcaaagaaa cgaagcaaga cttgcttacg cttgctttct tgggaaatga catagtccca
    20821 gtccagccca gtttcagtga aaaatttgct ggccttttga cgaaagtgga aatattttcc
    20881 tacccccaat aaaagcacat agagcagaaa aactggcaag ccataaccca ttgctaaaaa
    20941 taaaggcgca aataacagca agaaaagggt ctgtacaaag agccaaaaga ctagggaaat
    21001 gccagtttga cgcttgagat ggagcttgat ttcctcttct ccaactaaga gaaagagctt
    21061 gtctggagcc tccatatagg tggcaattcc tccccaaagt aaaagtaaaa cagacgtaat
    21121 tcctacaaac aaaaggatag gccaatgatt ttcaggaaaa tgttgtaaga gttgactgta
    21181 ctggtaggct agaaagccca acaggacaag caggaacaag acaaagtggt cattgagcac
    21241 atagcgcaga taaccaaggc actccttacg aaaggcctgc tttctcttta aaaacaagtc
    21301 tttcatagat cctcctcttt ggtcagagcc aagtaaatat cattcaaact agcctcaggc
    21361 atatcaaagg cttcacgtag ttgcaggaga ttgcctttgg cacgcacctc tcccttgtga
    21421 agaatgacaa aggcatcaca catcttctcc gccgaatcca gcacgtgggt actcatgaga
    21481 atagacttgc ccttttgctt ctccacttcc aaaagctgaa tcaaatcaga aatagccagc
    21541 ggatcaagac caaggaaagg ctcatccacg atgaaaagac ttggatccac cacaaaagca
    21601 cagataatca tgaccttctg cttcatccct tttgaaaaat gaacagggaa ccagtctaat
    21661 ttctgttcca aacggaacat ttttaacaag ggctctactc gttcgaaagc cactttttgc
    21721 tcaataccgt aagccatagc aaccgtttcg atatgctctc tgagggtcaa ttcctcatac
    21781 agactaggcg tctcaggaat gtagccaatc tgcttgcggt agctagtcgc atctccttgc
    21841 agagtcaggc cattgatatt gatggagcca ctataaggtg ccaacagacc gataatctca
    21901 ttgatcgtcg ttgatttccc agcaccattg agaccaatca aaccgaccaa ctgcccactt
    21961 tcaacagtaa aggacacatc tttcaaaaca gggacatgaa catagccacc tgtcaggttt
    22021 ttaatttcta acatattttc tccaaatctg gtataatgta gctatattat atcaaaattc
    22081 agtacagtag aggtagattt tatgtcagat tgcatttttt gtaaaatcat cgcaggggaa
    22141 attcctgctt caaaagtata tgaagatgag caggtccttg cctttcttga tatctctcaa
    22201 gtaacactag gacacacctt ggtcgtgcca aaagaacact atcgcaatct tttggagatg
    22261 gatgctacga gcgccagcca actctttgcc caagtaccaa aagtagctca aaaagtcatg
    22321 aaagtcacta aggctgctgg tatgaatatc atttccaact gtgaagaagt cgctggtcaa
    22381 acagtttttc atactcacgt tcaccttgta cctcgctaca gtgctgacga tgacctcaag
    22441 attgatttta tcgcccacga accagacttt gacaaacttg ctcaagtcgc tgaaaccatc
    22501 aaaaacgctt aaggagttgc tatgaaatta tccaacctac tgctatttgc aggagctgca
    22561 gccggaagtt atctggtcac aaaaaatcgc caaaccatca cagatgaagt cttgaatacc
    22621 actgaccgcg ttcaagctat caaggacgat gtggatatta tccaaaacag cctgcaaatc
    22681 attgaccagc aaaaagaact tatcaaggaa taccaagaag acttgactta caagtttaag
    22741 gtcttggaaa aggatatcca gactagacta gctgtgataa aagaaatgca gggaactgaa
    22801 gataagtaaa aagagccccc cggctctttt ttaaaacgta aacaagttat agtgaattga
    22861 atttagaata gcacatcata gtttctaaag cattgttaga gattacttta aaatcctctt
    22921 atcaatttgt tcatattcta tttcaatcta ctatatatat cccatcaact atgcatcata
    22981 atttaggtaa ctcatactca ataaaaatca aaaagcaaac tagaaagcta ggcacagact
    23041 gctcaaaaca ccgttttaag gttgtggata gaactgacga agtcagctca aaacactgtt
    23101 ttgaggttgt agatacgact gacgaagtca gctcaaaaca ctgttttgag gttgtggata
    23161 gaactgacga agtcagtaac catacccatg acaagccgac gctgacgtgg tttgaagaaa
    23221 tttttgaaga gtatcattca ccatttcaca actaaatcga agactttctg ccgtaatgaa
    23281 aacacctgaa acagcttggt ttcggctgtc cggaaacttt aagacttagg ttcaaagttt
    23341 aggtatggaa cttcgaagga ggtcgctacc gtccgtcatt acttaggaaa gtcttaaaaa
    23401 atcggctcta taaagtaaaa agagcccgac agctcttttt gtttatattc aatgaaaatc
    23461 aaaaagcaaa ctaggaaact agccacgggc tgctcaaaac actgttttga ggttgtagat
    23521 acgacaagcc gacgctgacg tggtttgaag aaatttttga agagtattat tctccatcaa
    23581 aggcatcttt aatatggtca aagaagcctt ttttctttgg atttactttc aagtcaccag
    23641 cagccgcgaa ttctttcaag gctacttttt ggcggtcgtt caagcctgtc ggtgttacga
    23701 cattaacagt aacgtattgg tcaccaactg caccgccacg aaggctcggt gcccccttac
    23761 tacgtaggcg gaatttctta ccagtctgag ttccctctgg aataaccaat tcaacatcac
    23821 cgtgaacagt tggaatatct actgtatcac caagagccgc ttggacaaag ttgaggttga
    23881 gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc gctagcttct acagaaacta
    23941 ctacatacaa gtcaccataa ggtccaccgt taaagcctgc ttcaccttga ccagcgaggc
    24001 gaatttgttg acctgtttcc acaccagcag ggattttcac atgtacgcta tgagcttgtt
    24061 tctcatgacc tgttccatga caggttgtac atggatattt gatttctttt cctcgaccgt
    24121 gacagacatc acaggttact tggcgacgca tcataccaag aggagtctgc gtatcgacgt
    24181 taatgacacc agcgccatga cagcgtccac aagtgactgg acttgtccct ggcttagcac
    24241 cagagccatt acatgtacga cagccagctt cacgatgata cttaacttcc ttctcagttc
    24301 cgaagatagc ttcttcaaag gtcaaattga cacgatactg gagatcatct ccttggcgag
    24361 gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa acttgagaaa atatcctcaa
    24421 aaccaccgaa accacctgcc ccattgaaac cgccgaaacc accagctcca ccaaaaccac
    24481 cattggcgcc tgcagcacca tactggtcat aggcagcacg tttttggtcg tcactcaaag
    24541 tctcataggc ttcttgaact tccttgtact tgtcctcagc accaggctcc ttgttgatat
    24601 ctgggtgata ttttttggaa agcttacgat aagccttttt gatttcgtct gccgaagcgt
    24661 ttttggatac ccccagacga tcataaaatt cagtattgtt cataattcaa gatacaaagg
    24721 gcgtcaaacg gacacagcca tctaggagtt ttgacgacgg acgcttacgt cctagaaaaa
    24781 actatctttt gggcacagtc cgtagcccgt gttcagttac gaacaccctt attcctttct
    24841 attgatgttc tgaatcgaac caccatttag gtcgggttca attcctttct tttatattta
    24901 gtaagaaatt ctgaaacccg tgttcagtta cgaacactct ttgttccctt ctattgatga
    24961 gacaaaaccc aaacaaatga gattccgtcc ctttttatcg aaaaacagag gctgggttgc
    25021 aacctctgca aataagtaaa ttaactttat tttgatttta aatatcctat aagtacagaa
    25081 atatttatat atttttgtct cttcttagta tttgcctata taattccaat tgatcataag
    25141 aaatacaact actgtccaaa tcataccaat tatgaacggt attacaaatt tatatgtttg
    25201 tatttctaat gatatcatag ctggtaccat cgccgataca aatgcagaca acaatactga
    25261 ggtaacaaaa ttccttaccg cttcatcatt tttgatacaa ctatcatgtt cagctaatat
    25321 aatttctcta tatctagcta aatattctat tggaatatta tcaataaaat cttttgatgt
    25381 tctatagtta tcagcttgat tacaaaaatc attataaact tttcgtgtat tttttagata
    25441 ctcttcatcc aatacactca tcttactttt ccgtaaactc tccgtctacg acgtcatcgc
    25501 ctgcgttccc tgttgcttgt gcgccttctg ctccttcttg agcttgttgc gctgctgcgg
    25561 cttgttcgta gagtttaaca gcaagtcctt gagctttttc gttcaatgct tcaagttttg
    25621 ttttcatgtc gtccaagttg ttgtcttctt gagctttctt aaggtcatca agggcagctt
    25681 gggcagcgtc acgttctgcg tcgaagcctt taccttcagt ttccttgatt gtcttttcag
    25741 tcgcaaagat tgcttggtct acttcattac gaaggtctac ttcttcttta cgtttcttat
    25801 cggcttcagc gtttgcttct gcatctttca tcatgcggtc gatttcttcg tcagtcaaac
    25861 ctgagttcga ttggatgaca atagtttgtt ctttttgagt tccaaggtct ttggccttaa
    25921 cagacacgat accgttcttg tcgatgtcaa atgttacttc gatttgagga attccacgag
    25981 gtgcagctgg gatatcagtc aattggaagc gtccaagagt cttgttatct gctgccattg
    26041 ggcgttcacc ttgaagaacg tggatatcaa cggctggttg gttgtctgct gctgttgaga
    26101 agacttgtga tttagatgtt gggattgtag tgttgcgatc gataagtttt gtaaatactc
    26161 cacccattgt ttcgatacca agtgacaatg gcgttacatc aagaaggaca acgtccttga
    26221 catcaccagt aatcacacca ccttggatag ccgcacccat agcaactact tcatcagggt
    26281 ttactgattt gtttggttct ttaccagttt cagctttaac agcttcaaca acggcaggga
    26341 tacgagttga accaccaaca aggataactt cgtcgatttc tgacaagctc aaacctgcat
    26401 ctgaaagggc ttgacgaact ggaacttttg tacgttcaac aaggtcacga gtcaaatcgt
    26461 caaatttcgc acgagtcaaa gtcatttcca agtgaagagg tccagcctca cctgcagtga
    26521 taaatggcaa gctgatttgt gttgaagtta caccagaaag gtctttcttc gctttttcag
    26581 ccgcatcttt caaacgttgc attgccatct tgtcagtaga caagtcgata ccgttttctt
    26641 tcttgaattc tgctaccaag tggtcaatga ttttttggtc aaagtcgtca ccaccaagtt
    26701 tgttgtcccc tgcagttgac aatacgtcga agacaccgtc acccaattca aggatagaga
    26761 cgtcgaatgt accaccacca aggtcaaata ccaagatttt ttcttctttg tcagtcttgt
    26821 ccaaaccata agcaagagct gctgcagttg gttcgttaac aatacgttct acttcaagac
    26881 cagcaatttt accagcgtct tttgttgctt gacgttgagc gtcgttgaag taagccggaa
    26941 ctgtgataac agctttggtt actttctcac caaggtagtc ttcagcgtag cctttcaagt
    27001 attgaaggat catagctgag atttcttgtg gagtgtattc ttttccattt gcagaaactt
    27061 tttcagaagt tcccatctta gatttgatag agataactgt atctgggttt gtaactgctt
    27121 gacgttttgc agcatcacca acgatgattt ctccgttttt gaatgagact acagatggag
    27181 ttgtgcggtt tccttctggg tttgcgatga ttttgctttc agttccttca agaactgcaa
    27241 ctgctgagtt tgttgtacct aagtcaatac cgataatttt agacatgtgt ttttctcctt
    27301 gttagttaat tttctatttt tctttttgat actcttctta agtggcgacg ccgtcagagc
    27361 ttgacttcgt cagtctcttt tactaaactt tgagcctaag gtctcaaaat ttgcgcaaat
    27421 agcgccacgg cgaagagtat cgtctttggt tcgcttcgct cactattaca aagcaaaaca
    27481 tattttatct ttcttcatag tttattgtcg tttcggacaa gttttctatt atgtaaattg
    27541 cgacacgagg agtcgaaatc gattttattt cgacgacgag ttagtaagga agctaggcaa
    27601 acgccatagc aattgccgtt ttctgacgag ttgctttagc taccgtcaga attgccgaat
    27661 cgaacacgcc ctaagctctg agtaaaaaag atagattttc ttgtgttcat cgaacacttc
    27721 gtcaatctcc tatttttact gcgagctttt tacgggcttt gtatcttagt tatacaccac
    27781 taccattgct gggcgtagga tgcggtcatg gagtttgtag cctttttgaa agacttgggc
    27841 gatggtatct actgggtgtt catcgtctgc tgggagagtt tggatggcca tatggtagtt
    27901 atggtcaaat tcgccatctg ctgcgatttc ttcaattcct tcttctttca aagcgtgaat
    27961 caagctttct tgcaccatcc ccaagccctt cttcacatca tctgtcaaac cttcaactgc
    28021 aagtgcacgc tcaaggttgt caagagatgg taagattgct tttgccaagt cctggctacg
    28081 ataacgttgc aagttttgac gttcttcatt ggcacggcgt tggatatttt gcatttctgc
    28141 atgagcgcga agatatttgt tttcgaactc atctgcacgt tcatttgcca agtccaactc
    28201 agacttttca ggagttgttt cttcagctgt ttccacaact tcctcttctt gaacttcttc
    28261 tacttcttca ttttttatat cttgggccat gttcgcctcc tttaatgatt tcaatcttaa
    28321 tgtacttcta atgatttcaa tcttaatgta cttcgtaatg attactgctg aggtagcggt
    28381 aaaaatctgt caacttcatg gtcaaaacac ggttgaccac attgatttga ttgattagct
    28441 gttggtaatc cagattaact ggaccgataa tggctagaat tccaactccc cgataaggaa
    28501 tgaggaactt actactaatc accgctaggt cagctaaaca ggactcttga ccgtctgcaa
    28561 cacgaacatt ttgcatctga tcctcacgca acccctcacg aatctccaag gccacctttt
    28621 gcggttggtc aaagaactga taggctgcta gattggcaaa attcaagaga ttgaccttgc
    28681 ccgccatcac aatgttttcg ttgaacattt ccttaaagat gtgttcaaag agatcgatga
    28741 cattatccgt tgttgtaaag taacgctgga taatctgcgg aatctccgtc cgaatcttgt
    28801 agtgaatatc taaaacggtg tgaccgagga aacgttcctg aatgatgctc ttcagtttca
    28861 gcaaatcctc ctgcaagaag ttccttggaa tcagaaactg actagtaacc gttcgcgact
    28921 cgtctagggt aaataccgcc aaggctgtat gttgccccaa aacaacgata tcaaaggctg
    28981 tcaaacgttg cctgctcggc tcaacatcca gtgccactac cgtacagcca ctcaggtctg
    29041 ttagcaagtt agcagcctct tgcagaatat cctccaattt gaagaattcc tgatcaaagg
    29101 ctttgacaat ctcatatacc tcattttcag ccagtcggtc aaaatccagt gagtgtttca
    29161 catagtactg aaaaccagca acacttggca tccgaccgct tgaagtatga gccttctcaa
    29221 gcaacccttg tttttctagt tccgccatgt cattacgaat ggttgcactg ctagagttaa
    29281 tagactcttg caaggctttt gatccgacag gttcgtgcgt tttggtaaag atgtcaataa
    29341 tcagatttaa aatatcctgc tgacgctctg taaccatctc atcactcctc tctgtctgat
    29401 caattagcac tcaatgcatc caagtgctaa cttatgattc tattatacac ccttagaaga
    29461 gaatgtcaag acaaaaactc aaaaaattag cactctttta taaagagtgc taaaaatcaa
    29521 tctgaagacc tagaaccttg ctattcatct actcggtatt tcctttttag tctgaaataa
    29581 ccatgaatta gataagaaat cagaaaagca tataaaacaa aaataagaaa gaaagacata
    29641 gaaagatcct ctctaaacaa actcatacaa acaacaagac cacctaagaa agtagctata
    29701 caggaacgaa gtctagatcg cataacttcc catttgagcc ccttactcat atagacttga
    29761 tcctctggaa gtaaatcaga agacgattta cgaagaatcg aacaagaacc tgctcctatc
    29821 aattcccaac ctctatctct aaaatcttgc agttcacgct tatgctcttt aagaaatcta
    29881 gaatcataga tacgatagat aacatcgtcc ggttgacctt gctcaaaata gaacaaacca
    29941 aaacgactag ttctatacct ccaacctttc aaatgcatct catgtaaata ttcttctttc
    30001 ttgtccaaat caacaatggt gaaaatccga aattctactc tgctattcat tgtcttaccc
    30061 caaaattaga aaacatgcct agcgttattt attagataat tctttccact tttgactcaa
    30121 tctccaaaaa atataagaaa tctgaatcgc aaaaactatc aataaaaccc aatctattat
    30181 gaaaatcaaa aacacttccc aactgaaaga actacctcca gtgacaaact ttgagaaaaa
    30241 cggtagtaga gctaaaaaga gaagcaaaat aggaagcatc cgcattgtta aaatcctttt
    30301 ggcataaaaa aatctttatt taaacgaaaa tattatggca aaatttacgc cagtttttga
    30361 acggctgatg tagatatttt atactttcaa aatgtttaaa tgtgattatt tatttttgaa
    30421 aaatagctca ccagcccgac tgaaagtgct tatagaatga taataagtcg cctgccgaaa
    30481 acagcgaaaa atagcagtgt tatgcggaga taatctgacg cgatgcgaaa gtatattgca
    30541 tacttatttt caacaattta gcagagtatt tttataagtg tgatataata gaagtataat
    30601 ttgttctgat agtttatttt atggagaagt agatttttag aatgcggagg attcaatatg
    30661 gttgagttta taaagtctaa gaaagaaatg agtgaggagg atattaaagc aaatttcatc
    30721 actcctgcta ttgtatccaa aggatggaaa aatggtgagc atatcgctta cgaagaatac
    30781 ttcactgatg gtcgaattga agttagagga gataaggctc gtcgtaaaga aggaaaaaaa
    30841 tcagactatt cactgtatta ccaatttgga actcgaattg caattgttga ggcaaaggat
    30901 aataaacaca gcgttcgagc aggattacaa caagctattg aatatggaga gattttagat
    30961 gttccatttg tttattcttc gaatggtgat ggctttattg aacacgaccg tatcacgaga
    31021 gaagaacgtg agctggagtt agacgaattc cctactcgtg aagaattatt ttctcgtatg
    31081 acgaaggaaa aaggattgac gtacgaaatt acagaagcta tctcaactcc atactataca
    31141 gacgccttct caacgaaaac gccacgctat tatcagcaaa tagctatcaa ccgtactatt
    31201 gaaacagttg ccagaggaca aaaacgagta atgtttgtga tggcaacagg aacggggaaa
    31261 acgttcatgg cttttcaaat tattcatcgc cttcgaaaag ctggtttggc taaacgagtt
    31321 ttattcttag cggatagaaa catcttagta gaccaaacga tggctgaaga ctttaagcca
    31381 ttcgaaaagg taatgacgaa aattacacca aaacttttga ctgctcctga aaaattaaat
    31441 tcttttgaaa tttatctagg gctttatcag caactaactg gtgaagatgg aactgaaaca
    31501 cattatcaaa aatttgataa agacttcttt gatttaatcg taattgatga agcgcaccgt
    31561 ggttcagcta aggaaaacag taactggcgt aagataattg attatttcag ttctgcgaca
    31621 cagattggga tgaccgctac tcctaaagaa accaagaatg cttccaatac ggaatacttt
    31681 ggtgagccaa tctatactta tagtttaaaa cagggaatcg aggatggttt tttggctcca
    31741 tatcgtgtta tgagggttaa tttagatgtg gatgtggatg gttatcgtcc agaaactgga
    31801 aaagttgata ctaacggaca attaatagaa gataggtact acggcaggaa agattttgat
    31861 aaaaccattg tcattgatga tagaacgcaa agagttgcca agtttgtttc tgattatatg
    31921 aagcaaaaca atgcacgatt tgataaaaca attgtttttt gtgttgatat tgaccatgcc
    31981 gagcgaatgc gtgctgcact tgtaaaagag aatctagact tagtccaaga agactatcgt
    32041 tatgtcatgc aagtaactgg tgacaacgct gaaggaaaag ctcaactgga taactttatg
    32101 gatgtcaatt ctaattttcc cgctatggta acaacgtcta aattattaac gacaggagtt
    32161 aatgctaaaa catgtcgttt gattgtttta gactctaata tccaatccat gactgaattt
    32221 aaacaaatta ttggtcgtgg cacacgtctt tatcctcaaa aggggaaaga attttttacg
    32281 attattgatt ttcgaaatgt taccaatttg tttgctgacc ctgattttga tggtgagcca
    32341 gtgaaggtgc tggaaacagg tgcgaaaaca gtcagtggtt ccacgcccgg tttcgtagat
    32401 gaggaaggtg acccagtaga aaaatatatc gttacagaca agcaggttac cattcttaat
    32461 tctactgttc aagtattgga tgaaaacggg aaactgatta ccgaaagcct gaccgactac
    32521 actcggaaga atatcttagg tagctacgcc actttgaacg attttatcac agtttggcat
    32581 acggcggata aaaagaagct tatcttagac gaactttata aaaaaggagt ttatctagat
    32641 gctattcgag agtcggaggg aatatcagaa caagaaatcg atgattttga tttactccta
    32701 aaacttgcct atggtcaaaa agaattaacc aaaacggaac gtatcaataa gctcaaacaa
    32761 agcggatatt tatataaata tagtgaggaa gcgcgtgctg ttttggaaat tttactgaac
    32821 aaatacatgg ataaaggtat tggagaactc gaaagcattg aaacattaaa acttccagaa
    32881 tttcagatat atggtggaac cttcaaaatc atcaatactt attttggaga taaaaaacga
    32941 tatttacaag caattaaaga attggagcaa gagctattta cagtagctta atgaaaggaa
    33001 agtatgtcaa ttacatcatt tgtaaaaaga attcaagata tcactcgaaa cgatgctggt
    33061 gttaatggtg atgctcaacg tattgagcaa atgtcttggt tattattctt aaaaatttat
    33121 gatagccgtg aaatggtttg ggaattagaa gaagacgagt atgagtcaat tatcccagag
    33181 gaattaaaat ggcgaaattg ggctcatgct caaaatgggg aacgggtatt gacaggcgat
    33241 gaattacttg attttgtcaa taacaagtta ttcaaagagt tgaaagagct tgaaataact
    33301 tcaattatgc ctattcgaaa aacgattgtt aaatcagctt ttgaagatgc gaacaactat
    33361 atgaaaaatg gcgtcttgtt acgccaagtc atcaatgtta ttgatgaagt tgatttcaat
    33421 agccctgaag atcgtcattc gtttaatgat atttacgaaa aaattcttaa agatattcaa
    33481 aatgctggga actcaggaga attttatacg ccacgtgcag cgactgattt tattgccgaa
    33541 gttcttaacc caaaacttgg agaatcaatg gcagaccttg cttgcggaac aggaggcttc
    33601 ttgacttcga ctctgaaccg tttaagtagt caacgtaaaa ctagtgaaga taccaaaaaa
    33661 tataatacag ctgtttttgg tattgaaaag aaagcatttc ctcatctttt agcagttaca
    33721 aatctgtttc ttcacgaaat tgatgaccct aaaattgttc atggaaatac tttggagaaa
    33781 aatgttcgtg aatatacgga tgatgaaaaa tttgacatta ttatgatgaa tccacctttt
    33841 ggagggtcag aattagaaac aataaaaaat aactttccag cagaattacg gagttctgaa
    33901 acagctgatt tatttatggc tgtcattatg tatcgtttga aagaaaatgg tcgtgttgga
    33961 gttattttac ctgatggttt tctatttggt gaaggtgtaa aaactcgctt gaaacaaaaa
    34021 ctggtagatg agttcaactt gcatacgatt attaggttgc ctcatagtgt ctttgcaccg
    34081 tatacaggaa tccatacgaa cattcttttc tttgataaaa caaagaaaac agaagaaact
    34141 tggttttatc gtttagatat gccagatggt tataaaaatt tctcgaaaac taagccgatg
    34201 aagtcagaac acttcaatcc tgttcgtgac tggtgggaaa atcgtgaaga gattctggaa
    34261 ggtaagttct acaaatctaa atcatttaca cctagtgaat tggctgagtt gaattataat
    34321 ttagaccagt gtggctttcc aaaagaggaa gaggaaatct taaatccctt tgagttgatt
    34381 cagaattatc aagcggaaag agcaacttta aatcataaga ttgataatgt attagctgat
    34441 attttgcagt tgttggagga caaataatga caccagaaca acttaaagca agtattctcc
    34501 aaagagcgat ggaagggaaa ttagtgccgc aaaatcccaa tgacgaacct gcaagtgaat
    34561 tattaaagag aattaaagct gaaaaagaaa aacttatcag tgaaggaaaa atcaaacgag
    34621 ataaaaagga aactgagata tttcgtggtg atgatgggaa acattatggg aagtttgctg
    34681 atggaagcac tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga
    34741 ggttttctac attggttgaa attgtcagag gtggctctcc acgaccaatc aaagattatc
    34801 ttacttctga agtagatgga ataaattgga taaaaatagg tgatactgaa aagggtgaaa
    34861 agtatataaa taatgttaaa gaaaaaatca aaaaatcagg gcttaacaaa actagatttg
    34921 taaaaaaagg tacatttttg ttaactaatt ctatgagttt tggtagacct tatattttga
    34981 atgttgatgg tgcaatacac gatggatggt tggctatttc gaactatgaa aactcattaa
    35041 ataaagatta cctattctat attctttcat caaatgtagt ttattctcaa tttctatctc
    35101 taattagtgg agctgttgtg aaaaacttga atagtgataa agttgcttct attcttatcc
    35161 ctctcccccc actatccgaa caacaacgca ta
//
LOCUS       DBERYS010000018        34987 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.018, whole genome shotgun sequence.
ACCESSION   DBERYS010000018 DBERYS010000000
VERSION     DBERYS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.018"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(381..1361)
                     /locus_tag="ACL8LA_001008"
     CDS             complement(381..1361)
                     /locus_tag="ACL8LA_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGS1812781.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1678..3693)
                     /gene="recG"
                     /locus_tag="ACL8LA_001009"
     CDS             complement(1678..3693)
                     /gene="recG"
                     /locus_tag="ACL8LA_001009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGS1812782.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3712..4815)
                     /gene="alr"
                     /locus_tag="ACL8LA_001010"
     CDS             complement(3712..4815)
                     /gene="alr"
                     /locus_tag="ACL8LA_001010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGS1812783.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4805..5167)
                     /gene="acpS"
                     /locus_tag="ACL8LA_001011"
     CDS             complement(4805..5167)
                     /gene="acpS"
                     /locus_tag="ACL8LA_001011"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGS1812784.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            complement(5212..6243)
                     /locus_tag="ACL8LA_001012"
     CDS             complement(5212..6243)
                     /locus_tag="ACL8LA_001012"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS1812785.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6245..7276)
                     /locus_tag="ACL8LA_001013"
     CDS             complement(6245..7276)
                     /locus_tag="ACL8LA_001013"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS1812786.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7357..9870)
                     /gene="secA"
                     /locus_tag="ACL8LA_001014"
     CDS             complement(7357..9870)
                     /gene="secA"
                     /locus_tag="ACL8LA_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGS1812787.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10016..10303)
                     /locus_tag="ACL8LA_001015"
     CDS             complement(10016..10303)
                     /locus_tag="ACL8LA_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812788.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10331..10963)
                     /locus_tag="ACL8LA_001016"
     CDS             complement(10331..10963)
                     /locus_tag="ACL8LA_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1812789.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10967..11608)
                     /locus_tag="ACL8LA_001017"
     CDS             complement(10967..11608)
                     /locus_tag="ACL8LA_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812790.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11601..12392)
                     /locus_tag="ACL8LA_001018"
     CDS             complement(11601..12392)
                     /locus_tag="ACL8LA_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812791.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12414..12806)
                     /locus_tag="ACL8LA_001019"
     CDS             complement(12414..12806)
                     /locus_tag="ACL8LA_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812792.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            13133..13984
                     /locus_tag="ACL8LA_001020"
                     /note="rgg1518"
     CDS             13133..13984
                     /locus_tag="ACL8LA_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator
                     Rgg1518"
                     /protein_id="HGS1812793.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(14216..14566)
                     /locus_tag="ACL8LA_001021"
     CDS             complement(14216..14566)
                     /locus_tag="ACL8LA_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812794.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(14706..15041)
                     /locus_tag="ACL8LA_001022"
     CDS             complement(14706..15041)
                     /locus_tag="ACL8LA_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812795.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(15129..16439)
                     /gene="der"
                     /locus_tag="ACL8LA_001023"
     CDS             complement(15129..16439)
                     /gene="der"
                     /locus_tag="ACL8LA_001023"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGS1812796.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(16453..17166)
                     /locus_tag="ACL8LA_001024"
     CDS             complement(16453..17166)
                     /locus_tag="ACL8LA_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGS1812797.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(17163..18059)
                     /gene="dnaI"
                     /locus_tag="ACL8LA_001025"
     CDS             complement(17163..18059)
                     /gene="dnaI"
                     /locus_tag="ACL8LA_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGS1812798.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(18060..19229)
                     /locus_tag="ACL8LA_001026"
     CDS             complement(18060..19229)
                     /locus_tag="ACL8LA_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1812799.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(19230..19703)
                     /gene="nrdR"
                     /locus_tag="ACL8LA_001027"
     CDS             complement(19230..19703)
                     /gene="nrdR"
                     /locus_tag="ACL8LA_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGS1812800.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            19843..20208
                     /locus_tag="ACL8LA_001028"
     CDS             19843..20208
                     /locus_tag="ACL8LA_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1812801.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            20213..20908
                     /locus_tag="ACL8LA_001029"
     CDS             20213..20908
                     /locus_tag="ACL8LA_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812802.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            20925..21692
                     /locus_tag="ACL8LA_001030"
     CDS             20925..21692
                     /locus_tag="ACL8LA_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812803.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(22045..23244)
                     /locus_tag="ACL8LA_001031"
     CDS             complement(22045..23244)
                     /locus_tag="ACL8LA_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812804.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(23241..24134)
                     /locus_tag="ACL8LA_001032"
     CDS             complement(23241..24134)
                     /locus_tag="ACL8LA_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812805.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            24257..24448
                     /locus_tag="ACL8LA_001033"
     CDS             24257..24448
                     /locus_tag="ACL8LA_001033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812806.1"
                     /translation="MKIKEQTRKLAVDCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYTYGKAKLTWFEEIFEEY"
     gene            24841..25029
                     /locus_tag="ACL8LA_001034"
                     /pseudo
     CDS             24841..25029
                     /locus_tag="ACL8LA_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558115.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            25444..26061
                     /locus_tag="ACL8LA_001035"
     CDS             25444..26061
                     /locus_tag="ACL8LA_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGS1812807.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(26381..27268)
                     /gene="scrK"
                     /locus_tag="ACL8LA_001036"
     CDS             complement(26381..27268)
                     /gene="scrK"
                     /locus_tag="ACL8LA_001036"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGS1812808.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(27417..29300)
                     /locus_tag="ACL8LA_001037"
     CDS             complement(27417..29300)
                     /locus_tag="ACL8LA_001037"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGS1812809.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            29677..31131
                     /locus_tag="ACL8LA_001038"
     CDS             29677..31131
                     /locus_tag="ACL8LA_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS1812810.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRTSEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            31112..32077
                     /locus_tag="ACL8LA_001039"
     CDS             31112..32077
                     /locus_tag="ACL8LA_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1812811.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKPAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(32112..33386)
                     /locus_tag="ACL8LA_001040"
     CDS             complement(32112..33386)
                     /locus_tag="ACL8LA_001040"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGS1812812.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(33386..34558)
                     /locus_tag="ACL8LA_001041"
     CDS             complement(33386..34558)
                     /locus_tag="ACL8LA_001041"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGS1812813.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(34689..34937)
                     /locus_tag="ACL8LA_001042"
     CDS             complement(34689..34937)
                     /locus_tag="ACL8LA_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812814.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPISQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
BASE COUNT        10344 a         7751 c         6520 g        10372 t
ORIGIN      
        1 aacatatata cggcaaggcg aagctgacgt ggtttaaaga gatttttgaa aagtattaac
       61 acctacagac aagcctgacg atagcaagaa ctaccctact cgataggtat cggcttttgc
      121 tttctgaaaa aaattatttt aagcaatttg acaaatctag caacaaaaaa ttctataaat
      181 ataatagatt gaaactagaa tagtacacat ttgcttctaa aacattgtta gaaatcgatt
      241 tgactgtcct gatcgttttg tcctattctt gtttcatttt actatatttc catgataaaa
      301 cgcatagtat caagtttttt taatcccctg atactatgcg tgtttgtaat ttttaagatt
      361 ttgtgcttag agtcgactcc ttattttaga tatttaaaag gaatctcact tccacagagc
      421 cagttgtaga cttggtcatt gacaaataca ttcatggctt cgtgagcata ctcaggcatg
      481 atgcgatagg ttttatcgca ggtcagacga ttataaatcg caaactgggt aatgggatag
      541 caaacatcgt cgtccaagcc cgtaatcatc ttaacctcac cttggatacg atgggcaaga
      601 tttttgacat cgatataggc aagggtcgcc atgatttcct cctctgtttc atggaagggg
      661 tcgtgaaact tgaaataacg gaaaagttcg tcgtaagcct cgctagtatt accaatctca
      721 atcacccgtc tgaagtctga caagaaggga taaatggcaa ctgttttctg aattcgagga
      781 ttgagcgctg ctgcaactag agctagagcc cctccttgtg aggcaccata gctagaaaga
      841 cgcttctcat caacctgaga cagactagca acaatttcga ccaactggta aatatccaga
      901 taaacatcct tataaaagag gtggtcccga ccttccatag caccacggat aatatgcccc
      961 ttcacggtat ttcctaaagg agaacgcaag ccgtcttgtg agtaacctga ctggccccgc
     1021 acatccatgg aaacaacacc gtaaccagct acggtgaagc ccagcatgtc ggcccagtcc
     1081 cagccacgtc ccatataacc atgaaaatgg aagattaatg gaaccttctc ctcactcttt
     1141 ggaagaacaa tgcgtgcata gacctttcct tccttgcttc cttcaaatgt taactcatag
     1201 cacttgactt gaggaatgtg gaaatctctt tcctccaagt ggtaggatgg aagtgtggaa
     1261 acatttttca cttccccatc ccagaaagca tcaaagtctt ccggaacctc atcccttcct
     1321 ctataggtct taatttcttc tagcaaagct ggatttttca tagcatgctc cttttatttt
     1381 gtaatcgtta ccacaactgt agcatatcta tgtaatcaat gcaagaaaga agggtaaata
     1441 gaaagtgaat ttagattctt tacttttagg atgatgggct gaaagtagtt ttagaaacga
     1501 aaaaagagca tttcgctctc tcttctgttt atactagttt tttgcttcct tcttttggaa
     1561 agtggtttgg taatttactt taatcgttac tgtcatatga gagtcctcgt ttctttttga
     1621 tgactttagt ataagagcca aacctaaaaa ctagcttaag ttttccttag agaaagctta
     1681 atccagatgt tctttctttt ccagatgaag ggcaatcatg cgccactctg gatcttcttg
     1741 ccaagcttct atagaactaa tgtagctagc aacctttctt gcttcttcta aaatcggaaa
     1801 atcttcgata atatcagcca cttggaactc tggaagtcct gactgtctgg ttccaaaaat
     1861 ctcaccagaa ccacgcattt tcaaatcttc ctccgcaagg acaaatccat tggtcgtttc
     1921 tgtcatgata cgcatgcggt ctttcccaga atccgtcttg ggattagcaa cgagaacagc
     1981 gtaggactgc ttgtcccccc gaccgacacg acctctaagc tggtgaagtt gactgagacc
     2041 gaagcgatcg gcatccatga taatcatgac agtcgcattg ggaacgttga ccccaacctc
     2101 aataaccgtc gtcgaaacca gaatatccgt ctttctctcc ttgaaatcct gcatgatctg
     2161 gtctttttcg tcactcttca tcctaccatg tagaagagcc acctctgcct tgcctgcaaa
     2221 atgagtcgtc aactcctctg ataaggcaat ggcatttttc aaatctagag cttctgattc
     2281 ttcaatcaaa ggagagatga cataggcttg ggaacctttt tgaatttccc cctctaacca
     2341 agtcaagacc tgaggtagtt gctcatgttt gatccagcgc gtcacaatag gcttccgacc
     2401 tgctggcatc tggtcgataa tggaaacatc catatctcca aaggctgtga tggcaagcgt
     2461 ccgtggaatg ggagtcgccg tcatcatgag gacatctgga ttgtcacctt tttcccgtaa
     2521 aatacgcctt tgccctacac caaaacggtg ctgctcatcg ataataatca aagcaagacg
     2581 agcatactcc accccatctt gtatcagagc gtgagttccg ataatcaaat cagcctcacc
     2641 cttggcaatg gtctccaaga cctctctctt ttctgcagct ttcaaggaac ctatcaagag
     2701 agccagtttc aaattgggaa aaaggttctg taaactctca aagtgttgct ctgcgaggat
     2761 ttctgttggt accattaggg cagcctgata acctgctgtc actgccgcaa acatggccaa
     2821 gccagcgact accgtttttc cactccccac atccccttgt aggagacgat tcatgtggtg
     2881 gtcggacttc atatcagtta aaatttcctg caaactcttt tcctgagctt gggtcagggc
     2941 aaaaggaaga cttactttaa ctgctgtcac tttttcctga gaccaattca gaaccagacc
     3001 acttccctga actctatttt cagacttgag catctgcagc tgcatttgga aataaaagag
     3061 ttcctcaaac tttatacggc gaagagcctg cttgtattct gccaaatcct ttggaaaatg
     3121 catagctcgg actgcctgac aacgggacat gagtttgtat ttgtctagta aagactgggg
     3181 cagattttct tctatcaaga ggtccagtcc ctgatcaaaa gccgtcttga tgaccttgac
     3241 cagactggcc tgactgattc cctgagccag acgatagaca ggctggaggt catcttctac
     3301 ctgagccaga accttcatcc cagtcagact agccttagcg cggtcccatt ttccaaagac
     3361 agcaagggtt gctcccaact ctattttatc agccagatag ggctggttaa agaaattcac
     3421 cgcaaaaacg acctctccct gcttgagact aaaacgcagg cgattgcgct tgaaaccata
     3481 atactggaca ctagcaggag tcactacctg accagaaaga actgccttct caccgtcttc
     3541 cagctccagc acctgcttgg ttttgaagtc ttcataacgg aaaggaaagt agagcaagag
     3601 atcttgcaag ttttcaattc ctagtttggc gtatttttct gctgactttg gtcccacacc
     3661 aggcaagaca tgcaagggtt gatgtagatt catgctccac tcctttcttt tctaataata
     3721 ttctctcgga atacggtcgc tgaggaggca aaccacctca tagttaatgg ttacgcggta
     3781 ggtcgctacc tgagttgcag tgatttcctt atccccattg gagccaatca aggttacctt
     3841 ggttcctagc ggataaagct taggcaatcg aatagtgatt tggtccatcg aaaccctgcc
     3901 gacaattggg caagcttggc catctaccaa gacagagaaa ttttgcatgt ctcttgtcca
     3961 tccatctgca tacccgattg gcacggtcgc gatgacttgc tcgctatccg cttgataagt
     4021 tgctccatag cccatgcaag ctccagctgg aactgtcttg acatgaacca gagcagactc
     4081 caaggtcaag gccggtatca aatcataagg caaatccaag accgctccac ttggattgag
     4141 gccatacatg gcatctccca tacgaaccgc attgaaaata gtctctacat gccaaagagt
     4201 cgttgcagaa ttgctagcat gaaccagctc tggaacttcc ttcatactag ctaaaatagt
     4261 tttaaaccgt tctaactggg cattaaaata gtcatctgat tcctcatcag cagtagcaaa
     4321 gtgggtaaag attccttcaa cacaaacacc gtgttgttgg agcaaatctt gagcctgctc
     4381 aacctcactt gcctctctaa aaccaatccg tcccatccct gaatcaatct tgaggtggac
     4441 tgtcaatcca gttaggtcca cttccttatc taagagtgct tgaatccact ccagtccagc
     4501 cactgtcaag gtgaagtcat attctttagc tagagcaaca gcttcgattt cagaaactcc
     4561 taaaatgagg attggcttgc tgagtccagc ttgtctgagt tcaatggctt catcgatatt
     4621 ggaaacgcaa aagccatcaa catcatcttg aattgccttg gcaacggcaa cagctccatg
     4681 accataagca ttggccttaa ccacagccaa cttgagcgtt ccttgaggga tatgagcccc
     4741 catttgctga atattttgtc gaatagctcc cagatgaatc agagccttgg ttggtctatg
     4801 tggactagct ttcatgattt tcctccaaaa tgacactggc tgtcacaaac tgatcggtgt
     4861 ggctgataga cagccaaatc tttcctgaaa atggtgcctg actaaaataa ggcgccccac
     4921 gttcattgtt caagacttcc aaatcctgaa aaccgagctt gctaatgccc gttcccatag
     4981 ccttggaaaa ggcctcctta gccgaccagc gaccagctaa atattctatt tgcctgcgtc
     5041 ctttgagact ggtgaagcgc tccatttcct gagcggtcag tacacgctta gcaaatcctt
     5101 catgtcgtgt aactgcgctt tctatcgaag ccaattcttc gatgtcaatt ccgtgtccaa
     5161 ctatcattct catcaaaagg agttgagatt ccccaactcc atccttttca cttattttgt
     5221 caaggtaaca taaatctctt ctaccaaggc ctctgtattt tcccaaccta ggcaagggtc
     5281 agtaatagag caaccaaaga tctctggttg gttttgacga ccatctgcta ggtaagattc
     5341 aatcataaat cctcgaaccg tctttttaat tttctcattc caatcacgat tctgcaaggt
     5401 ctggcgaaca attcgaatct gctccatata ttgcttgcct gagttatcat ggttggtgtc
     5461 aatgaggata aaaggatttt caagtcccat ggtttcatag cgttcaatgg cttggagtag
     5521 attttcatag tagtaattcg gcatataatt gccatactcg ttgactgctc cacggaggat
     5581 aacatgggcc aaaggattac ctgatgtctc aacttcctgc ccatgataaa ggaaggtttg
     5641 cttgttttga gcagcataga tggcgttaaa cataacaccc aaatttcctg aggttggatt
     5701 tttcatccct actggtgcat caatcccaga agccacaaag cggtgctctt ggtcttccac
     5761 agaacgagct ccaacggcat ggtagctgac caagtcatcc accaagatca gatttgacgg
     5821 ataaagcatc tcatctgccg ttgtcaaacc agtctctgta atcacgcggt agtgcaactg
     5881 gcgcacagcc tgcaagccat taatcaggct tggagcctta gaagtatctg gctggtgaac
     5941 taatccttta tagccgtctc cattggtacg aggcttagca gtatacacgc gcatgaccat
     6001 gaaaatctta tccgctacct tcttttgcaa ggcagataaa cggcgagcat attccaagac
     6061 cgcctcttca ttatcagaag agcaaggacc aatcaccaag agaatacggt catcttcccc
     6121 tgaaataata tctgccaatt ccctgtcacg gctttccttg agtctcaagg cttccgcaga
     6181 caattgggtt tcagccttga tgacttccat atcgatttct tgaccttttt caataaatgc
     6241 catcttattc tcctaacgtt ttgtaaattt ctctgacaag agcttctgtg ttatcccaac
     6301 ccaggcaagg gtctgtgata gacttaccaa atacttctgg ctcattttgt cgaccatctt
     6361 ccagataaga ctcaatcata aaaccacgaa cgaactgctt aattttttca ttccaagcac
     6421 ggttaatcaa ggtctggcgg acaattcgga tctgttcaat atactgctta ccagaattgt
     6481 catgattggt atcaatgatg ataaaaggat tttccaagcc cattttctca tactgggcaa
     6541 tggtatcaat taaattgtca taatagtagt tgggaatatt ttttccatac tcattaagag
     6601 caccacgaag aatagcgtgt gaaagcgggt tcccagttgt ttctacttct tttcctaaga
     6661 aaaggaaact ttgtttgttt tgagcagcat aaatcccatt aaacatgaca ttgagatttc
     6721 cagaggttgg atttttaaaa ccagtagaaa atcctgcccc acttgccaca aagcggtgtt
     6781 gctggtcttc aactgaacgg gcaccaactg ccatgtaaga aatcaaatca tctacaagcg
     6841 gaaggttttc aggataaagc atttcatcag cagttgtcat ccctgtttct gtgatgacac
     6901 gatagtgaag atggcgaacg gctttgattc cattgataag actaggcgct tctgtcgcgt
     6961 taggctggtg aatcaagccc ttatagccat ctccgttggt acggggtttg gcagtataaa
     7021 cacgcataac cataaagata cgatctgcca cttcttcttg taggactgcc aaacgcttag
     7081 cgtattcaag gacagcttct tcgttgtcag atgagcatgg cccgattacc aagagaattc
     7141 gctggtcttc tccacgtata atggcttcta gctcttgatc tcgctgtgat ttcctctcca
     7201 aagcctgacc ttctaatttt gacaaggcac gaacttcttc tatatttatt ttagagcttt
     7261 ttgctgtaaa taccataact catctcctta taaagcaaac ggatacccag taaaaattta
     7321 cttttcacaa gatatccgcc tctaaactat ctcattttat tgtcttttac cgtgacagtt
     7381 tttaaatttc ttaccagaac cacatgggca aagttcattg cgtccaatct ggctcaaatc
     7441 caaatcttct ggcatacttg cttggtgagc agcgatattg cgagtcgctg ttgtactgat
     7501 atgacgttct gcctgtggtc tttcttgttc atgaatttgt gctttcatca tcaagcgtgt
     7561 cacatcaaac tcaatcgaac caatcatatc attaaacata cggaaacctt ctgcctgata
     7621 ctcaacaaca gggttgttct gagcatagcc acgaagtcca accgcgttac gcaattgatc
     7681 aagggcatcg atatgatctg tccacttgtt atccaccact cgtagaatca aaactttttg
     7741 gaattcttta actgcttctt catcgcgtag ttttgaaacc tgactatcgt aaaccttcaa
     7801 ggaacgttgg aaaagctctt ccttgatggc cttatcagac aagcctgaca agtcttccat
     7861 cgtaatagaa tcttcaggaa gcaagttgta cttagcaaag ttcaaaattg cctctagttt
     7921 ttcatcttgt ttggcacgcg catgaccatc aacgacacgt tcaatcgtgc gtttgatcat
     7981 agactgaatt tcaggtgcca agtcacgatc tgcagtgatg acatcgtaac gttgagcata
     8041 gataatctca cgttgttcac gcatgacatc atcgtattga aggacttgtt tacgggtatc
     8101 gtagttattt ccttcgacac gtttctgagc tgcttcaacc tgacgcgtca acatgcgaga
     8161 ctcaatggcc tcttcagaca tgttgagacg ttcaaagatt cccttcaagc gttcagaacc
     8221 aaaacgtttc atcaaatcat cttcaagaga taggtagaat tgtgactcac ctggatctcc
     8281 ttgacgacct gaacgtccac gaagctggtt atcgatacga cgactttcat gacgttctgt
     8341 accaataaca caaagtcctc caagttcacg aacaccttca ccaagcttga tgtcggtacc
     8401 acgacccgcc atgttggttg cgatggtaac ggcaccacgt tgaccagcat tcatgatgat
     8461 ttgggcttct ctatagtggt ttttggcatt caagacttcg tgaggaacac cagctgcaac
     8521 caatttctta gaaatgtagt cactagtttc aaccgctact gtaccaacca agacaggttg
     8581 acccttttgg taacgagcct taacgtcttc gacaaccgct ttaaacttag attcgatact
     8641 tgcataaaga aggtctgagt ggtcaatacg ttgaacagga cggtttgttg ggattggaat
     8701 aacacgaatg ttgtagattt cacgaaattc ttcttcctca gtcttacctg tacccgtcat
     8761 accagacaat tttttgtaca tacggaaaag gttttggtac gtgattgagg cagatgtctt
     8821 ggtttcatcc tggattggca caccttcttt ggcttcaata gcttggtgca atccatcaga
     8881 ataacgacga ccttccatgg tacgacctgt aaattggtcg acaatcaaga tttcttgctc
     8941 ttcgctcacc acatagtcaa tatcgagaag catgatgtag ttggcacgaa gggcgttatc
     9001 gataaagtgg gtcaaagcca cgttttcgat gtcatagagg ttttcaagtt tgaagtagct
     9061 ttcagccctg tcaatccctg aatcagacaa accaatagtc ttagactgca catcgatgat
     9121 gtagtcatct ttgttcaaag attttacata gtggtctgcc atgtgataca actgactggt
     9181 ttcaaccgca ttggcacctg atacaatcaa aggtgtacga gcctcgtcaa tcaagataga
     9241 gtcaacctca tcgaccaagg catagttaag cggacgttgt accatgtttt cagcgcgaac
     9301 gaccatgttg tcacgaaggt agtcaaatcc gatttctgag ttagttgagt aagtaatatc
     9361 acactcatag gcttcttttt tctccattgg agatttggta gccaagttaa tccctactga
     9421 caaaccaagc caagagtaca attcacccat ctcagtcgcg tcacgttctg acaggtattc
     9481 attaaccgta actacgtgaa cccctttacc tgaaagggca ttgaggtata ccggcatggt
     9541 cgcagttaag gttttccctt cccctgtacg catctctggc acgtcaccat ggtgaagaac
     9601 aatccccccc atgacctgaa ccttataagg gaagagacct aggacacgtt tggcaccttc
     9661 acggacaacc gcaaatgctt cgtaaagcaa tgaatccagt gattctccat tttgataacg
     9721 ttccttaaat tcaactgttt ttgcttttag ttggtcgtca gtcaaagcag ccatttggtc
     9781 ttcgtatttg aaaaccttgt cagccatctt ttccagacga cggatttctc ctttatcatt
     9841 ttcgataatt gtttttaaaa tattagccat gtttttcctt actttaaatt ccgaatattt
     9901 tagaatgttc ctttaatttt agcacaatta ctgattattt tcaaggaaga atccccattt
     9961 tgaaaaggaa aaagaggtta gttttcaagc taacctctcc gacttttatg actgtttaat
    10021 tgccaaaaat cggcaaaatg gcaaatgtga taaataagtt aatccaaaga gaaagacagc
    10081 aaatcagtcc aaaaacaaag agactgactt tcttggacag caaggccatg agaatggaca
    10141 gaattccaaa agctagcaag attttctgta tgatgaagat gtcaatcctt acccccagaa
    10201 tcgctctatc ccaagggata aagaagaaag acagccagat cagcagaact aaaccaatat
    10261 aaaccttgga atagcgtgta ataaatgatt ttagatgtgc cataagccat ctcctaggat
    10321 tgtatatcct ttacatttgt atatgtccat tttcgacaac taatacatct gtacaaaggt
    10381 ctgaaatatc ttccgatatg tgcgacgtca ggataaccag accattttct tttttcaggt
    10441 aagataaaat gacctgtttg gttaacctca cactcttctc atccaaagca ttcataggtt
    10501 catcgagaaa gagtatagaa ggagaggaaa taaaggcttg aatcaaggcc attttttgct
    10561 ttgttcccaa ggataaatga cggtattcaa cgtcttcaaa ttcctgtaaa tcatagtatt
    10621 gaatccaata ggcaattctt ttttccgtaa ctttagatga aaaatacctt aacagttcca
    10681 aattttctct cagggataaa tgagataaaa actcgacttt ttcaattaaa attcctgcat
    10741 cctgaatata atgatttttt atcccgtaga ctttcccgtc ttgaagaact tttcctttgt
    10801 caagcttaat atagccagca agtaccttta aaagaaccgt cttgccagat ccattatcac
    10861 ctttaagtcc ataaattttt cctgattgaa acaccaaatt caactgattt aaaataggtc
    10921 ttttcccata aattttctgt ccatttataa tttcaagtct catcgtttat cctttcttaa
    10981 atttctcttc caagcgacta acaatcaaca taactaataa acttaagagt agtaaataca
    11041 ctgttacaat atgatccatc atccattctt gtcccacaaa tactaaaaaa tagagaaaaa
    11101 aggaaaaacc agcgcccgca ctaatcagtg ctatcagata agacagtagt aagtacaaca
    11161 tcattaactg agcaagtaaa aagaaaagtg cgactgtttt gaaatgaatt cctagcggaa
    11221 gtaaaccaag gaaccagaca aacaaatctt gagtaaaaaa tttagaaatg acaaccaatc
    11281 gtttccttgt ataatcgaaa aaggagcttg attgatagcg aatcaagtga tggtaatggt
    11341 ctgccaaatc actttcaagt aaaatgacag agagccatcc taccatgaaa agcgaaaggt
    11401 ttaacaagag tttggggatt tctaaattcg cttcagaata gtcattatag gaaaaaattg
    11461 aaatcaattc tctcttgaaa aagataacag catcctgctt ttgaaccgct gttgacaata
    11521 gatggtttat caaaatcatc atcagaacta tacgaagtag taaaacaatc tttctcttta
    11581 gcaaaatatt gttaaaattc ttaaacatta tcttcatatt tttcaagcac catgtaaggg
    11641 acaatataaa ggatataagg agtaaagagt ttcatcaaac ttacatcccc atagctagct
    11701 tgcatcaaac tcgtcatagg aaccatatag taaggcaagg cactatagag cagcatagaa
    11761 ccaagccaaa gaaacatcaa ataggtgatt gccgaacgag tcacattccc cacataatca
    11821 actatttgta aaaaatagat tgcattgatg aaaataccga ttagcaggac acaagtaaag
    11881 aacaaatagc tttttatctc tccatctagg agtctttgta aaccacttcc atcagaaaat
    11941 agagaattcc atccaagagg agaaaaagtc ccaaggaaat aggttataat tgcaattgtc
    12001 agcacagtca ctaaatatat caaacaaggg atacttgcaa cttgcaaagt caactttctt
    12061 tttaaccctg gatagctcac ttctcttcca atactcaatc gtaaaacttt atttttcagc
    12121 tcaatatatt gaaaacctag caaaacaatg atgattggaa taataaatga ttgataaaaa
    12181 cctagattga actggagaat acgaaaatct aatgctgatt ttaataagtc atttttaatt
    12241 gcttgtccat cgaatttagc tagatcgggc gcatgaatat caaaaaagaa ataagtcatc
    12301 aaaacgtcac gataaaccaa agcaagaaaa agcaaagtaa gcaagacata aacaaattta
    12361 tgccttgcca aagttaagag aggaatcttc ataaattata cctactttac gaattaattt
    12421 ggtgaccaac tacctgaaat ggcagctgat aaatcccacc aattttgtct tgccatatct
    12481 gctgcataaa catagccaac cgttgcccaa ttaccatgag ttgaacgagt accacaggtt
    12541 gttaacgatt ctccactacg agcttctcca ttagaattta ctatacgatg cttaacagtc
    12601 atgggatgta aatttctaca aggatttaag ttcatgatat attccgcacc ttcattttct
    12661 ttagtcaaat aacctgttct cttataactg gtagtaataa caaaattact aaaagcacct
    12721 atctgatttg cttctacgaa agtagttcca acagcactta tcattaaaac aggaataacc
    12781 attttcaata atttcttaat tttcattttt tctgtttcct ttcatttttt attcaagaag
    12841 attccttata acttctattt taaatataga cttattctaa acgatggtat atcatacaaa
    12901 ttcctccttt acttattata tatagactct aatttcttta tccccaaaac catgtttcaa
    12961 accagataag ttgaatccaa ctccaaatca aaaatccaga gtttttcggt aaattcttca
    13021 aatatttttt aaagcccatg actttttccc tcctttttga tataataata ccaccttttt
    13081 ctacgttttt tataaatgta gaaaatattc acatttacct aggaggaaca atatgagatg
    13141 ggattatgga caaattttta aagaaattcg aaagtcaaaa ggattgaccc aacaagatgt
    13201 atgtggacaa gtcatacatc gaacaactct aacaaatatt gaacacggta aagttattcc
    13261 cagttttgaa aacatggtat ttcttcttga acaaattgat atgagcttgg cagaattcaa
    13321 gtatatatgc aacgaatacc atcctagtaa aaggcgagat attattgtag agagccaaaa
    13381 tccgtctact tttcaagata ctagaaaaat ggttgaactc actgagaaat gtcaaaaata
    13441 tcttaagaca catcacgatg ttcctattca aaatatctat cgtcatacaa aaattgtcac
    13501 agagttacga actaaaggat tcaaaaacaa ccacgtcttg aaagatttgt atgaagaaat
    13561 ttgggactat cttgaaccta tggatacatg gtacattagt gatttgaaat tgcttggaac
    13621 cattctcttt ttctttcctt ctgaaaatct tccccttctt attgatagaa ttatgaaaac
    13681 catcgagaaa tataaatact tccgagaaac aaaagcattt ttatcatctt ttttagccaa
    13741 tctctccact gtatattttc aacatcattt attcaaagaa tgtgaaacaa tcaccctaca
    13801 acttttggta ttagcagaag aattaaaaat atacgatatt cttggattct ctcaagtacg
    13861 cttgggaata ctacaacata actccgattt gattgataaa ggaataactt tattacgact
    13921 aaccaaagaa gaggcattag ttaaaatact cgaaaaagaa attaatgatt tttcaaacct
    13981 ataatttgta ataactctca tcattttatt atagtagatt gtaatagaat gtaatagaat
    14041 gtgaacgaat caattagggg atctaaagca ttttatacca atgttttata agttgtattg
    14101 tattattcca tattcaatcc actatacaag atgaatattt ctctcaagtt tcataatcaa
    14161 aataaaaatg actgtatcta tagctccata aacaaatcat agatacagtc attttttagt
    14221 tcctacacct tcctttatgc acacctttag atttccctag ttcccgcctc aagcgaatca
    14281 tttttttgaa aaagacatag gctaaccaat cactagcaaa agcgataagt agtaataata
    14341 gccaaatacc ccataacttg atatctgtca aatttctcaa gacgatattg aaaaacagaa
    14401 ctgaaaaaac tgtccaagca aggctaaaaa gagcatagag ggtgatgtaa atccagtaaa
    14461 aatagtaaaa aattgggaaa aatttacttt ctctattatc cttgtcaatc cagctgaaaa
    14521 agtaaaaaca gggtaataag attatcattt taaacaagtg ttccatcaca agcctccttt
    14581 tttaaattgt tttctttagt ttcgttctat taaatgttat taccaatata aaccttggaa
    14641 tagcgtgtaa taaatgattt tagaggtacc ataagccacc tcctacaaat agaaaccgat
    14701 ataaatcaat gccttccacc cttagacttc cctagttcct gtctcaagcg aaacatttct
    14761 ttgaaacagg aataagttaa ccaattcata ccaatagcta gcagaataaa aagaaaccaa
    14821 atgccccata acttgatatc tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa
    14881 acaactgtcc aagcaaggct aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa
    14941 taaaaaattg gaaaaaactt actatttctg ttggcctttt caatccagtt atcaaaataa
    15001 aagtacggtg ctaaaagtaa gaatttaaac aaatgttcca tcaccgacat cccccttctt
    15061 ttgatagcgt tttctattat tttattatat caaaaaaatc cggaactgtc attccagatt
    15121 ctactttttt atttgcgttt tcttgcgatg agatgaatcg gtgttccctc aaaaacaaag
    15181 gccttgcgga tttgattttc caagaaacgc aggtaagaaa agtgcatgag ttcttcttca
    15241 ttgacaaaga tgacaaaggt tggtggtttg gttgccactt gggtcgcata gaaaatcttg
    15301 agacgttttc ctttgtctgt cggtgttggg ttgatggcaa tggcatccat gatgacatcg
    15361 ttcaagacag ctgatggaat acgtgtattt tgactttcgc tgatttgctt aatcatctca
    15421 ggaagtttgt ggagacgttg cttggttaaa gctgatacaa agataatcgg tgcgtaaggc
    15481 aggtattgga actgctcacg gatatcttct tcccagtttt tcatagtgtg gttatctttt
    15541 tcaagcgtat cccacttgtt gaccacgata atcatccctt taccagcttc atgagcaaat
    15601 ccagcgatac gcttgtcgta ttcacgaatc ccctcttccg cattgatgac cattaagacc
    15661 acatctgaac ggtcaatagc acgcatggca cgcatgacag agtacttctc agtattttca
    15721 taaaccttac cagacttacg cataccagcc gtatcaatca tggtaaactc ttgaccatct
    15781 gtatctgtaa agtgggtatc aatagcgtca cgagttgttc cagcaacagg actggcaatg
    15841 acacggtctt ctcccaagat agcattaatc aagcttgatt ttccaacgtt aggacgacca
    15901 atcaagctaa acttaatgac atctggattt tcttcttcat attcatttgg aaggttttcc
    15961 acaatcgcat ctagtacatc ccctgtaccg ataccatgga cagatgagat aggcagtggt
    16021 tcacccaaac cgagagcata gaaatcatag atatcatttc tcatctcagg gttgtccacc
    16081 ttgttgactg cgaggataac tggtttgtgg gtcttataaa gcttacgagc tacgtattcg
    16141 tctgcatcag taattccttc cttaccagac acgacaaaaa cgataacatc tgcttcttcc
    16201 atggcaattt ctgcctggtg cttgatttgt tccatgaaag gagcatcgac atcatcaatt
    16261 cctcctgtat caatcatgct aaaagaacga ttgagccact cacccgttgc ataaatacgg
    16321 tcacgtgtca ctccttcgac atcttctaca atggagattc gctcaccagc gatccgatta
    16381 aatagggttg atttcccaac attgggacgt cctacaatgg caatagttgg tagggccata
    16441 atttctcact ttctacaata atttcttctg ttcaagattt tttctagttg agcttggttc
    16501 agcttgacca aactgttctg ctaggcgctg actccagctt gtggtcgcac gcgccccagc
    16561 atagtcagcc tgaacacggt cataagcttg gattgcctca gttgactgtt cttggtattc
    16621 ttcctcaaag acaacattct ctagtggcag tctcggtttc atatcatgat gttgatttgg
    16681 cacacccagt gccatcccaa agacagaata ggtgtagtca ggtaggttaa agagctctgc
    16741 cacttcttca gacttgtatc gaaccaaacc gataatcaca ccaccatagc ccaagctttc
    16801 agctgccaac aaggcgtttt gtccagcaag agctgcatcg accgaactaa tcaagagacc
    16861 ttccacacct tggggttgga aggtgtcggt atgaagtctg gctccctttt ctgctcggtt
    16921 caaatctccg acaaagagaa ggaaaacagc agactggcga atggcttctt gaggtaccaa
    16981 ttcatacaag gcatctttct tctcttgact tcgtaccaca atcacagagt aggattggaa
    17041 attcttccaa gatgatgcca tctgggctgc tgtcaaaatc tcatttaagt ctacttgggg
    17101 aatttcttgc tctttaaacc tgcgcactga agtatgagcc ttcatcaatt taatggtttc
    17161 tgtcatcgac ggtttactcc ttctaaacga gtctcctcag ccaaataacg gatgcgttcc
    17221 atgacccgtc tggcttccca ggtttcgtca tttccatgtt tcactttcgc aaaatgcttc
    17281 tccaaatctt caaagttgaa gttggatgtg aaaaaggtcg gtaaattttc ctgcatccga
    17341 tattggagaa tgacctgcag gatttcatca cgcacccaag cggtggattg ctcggcgcca
    17401 atatcatcta aaatcaggac ctcagacagc ttaatctcat ccaccaaggt cttaacattg
    17461 ccatcactga tagcattttt gacatcaatg acaaagctag gatagtggag gagagttgat
    17521 gaaacaccac gtttttctga taaatcatga gctaaggccg ccaccatgaa acttttaccc
    17581 acaccaaagt ctccatataa gtaaagacct tttcgaatag ctggatattg ctccacgaag
    17641 gctagtagct tttcaaaaac tggtaagcgc cccaaatcat ccaagtcaac ttgagccaaa
    17701 ctagctttct tgagactggc tggtagattg attaacttga gacggttctt aatagccgct
    17761 tctttttcag ccgcgattag ctcaggagtt tcttcatatg aaacatctgc atagccatga
    17821 ttcttaacca aaatcggctt gtagcctttg gcaatataat ccgtatcccc acggagaaac
    17881 ttgtcacgct cggtgatgta ctgattaaac ttggagatac tgcgatttaa ttcctctgga
    17941 gtcaaggatt cttgctggat aaaagccgca acatcagggt ccttcatgat tttctggacc
    18001 aaatcttgat aataaaaacg gctgggttga cgtttgagta cgtctccgac actttccatc
    18061 taatctcctc ctttttctaa tcgagctaat agttcttgct tcttacgttc tagttccaga
    18121 cgagtttcct cgctggtttc attcttatat tcaggattac tccatttagg aacattggtt
    18181 tttactgggg cagtctgatt ctgtttttgt gtttttgctt tctgtcctcg ctcacggatg
    18241 cgcaagactg ccttttctgc cgaatgaatc ttttgatagg catagtcatt ggctaccttc
    18301 atggcatatt tctcattgat atttgccgaa tcgaccttat taaaggtcaa taaaagaatg
    18361 atattgatga cttcgtccag caagcccaag ccagccatct gttgcaagag ttctctttct
    18421 gtttgggtaa tggttccctt gcgtgtttgc ttgatttctg ccaagaactg cagggcagtt
    18481 ttacttttgg cttctttgat aatggttgtt tccttaagac taaagtcaga ggaaactggt
    18541 ttttgagcaa ttttttcacg catgcgtttg gttgaaataa cctgagaaac agctgttgac
    18601 ttggccaatt gataggtttc aaaccaagtc catttcttct cctcggcaat agcaaagagg
    18661 tttaagacat cggactgctc atccgcaaaa cgaagtccat ctcgagccat cagctggcga
    18721 aaatgttcca agtcaaaatc attggccact ttcttcttga gaccaaggtc ttcttgactg
    18781 cctagttctg ccaattctgg aaagacttga ttgagtgaga caggtatttc ttcaccatca
    18841 gcactttcaa ctttcaaatc ctccacagca gagtcgccaa tctttttctc taagagtctg
    18901 cgataaacag gatgctccaa gaagtcttga ctagatagag gagcatggag ggctagctga
    18961 taaacatccc ccttttgata gagggtcaag agattaaaag cagataagat tttcaatgat
    19021 tttatcagtc tatccatccc aaagttgaga tgattgagga tacttgaaaa aagatattcc
    19081 tttctaccat tatcccaaaa actgattgta taaagataaa ggctcagtgc ctcctgaccg
    19141 ataatcggga ggtagcactg taccagagat gaggtatctt gcgacacccg attattcttt
    19201 agataagaaa aacggtcaat tggcttcatt tatctttcct ttttcttttt agaggactgg
    19261 gtgatttgtt ggagcaagct ctctaactca ctgacatcct taaaactacg atagacacta
    19321 gcaaaacgta cataggtaat ctcgtccaat tcagccaact cctccatgac gagtgaacca
    19381 atgtcctcac tttgaatttc attttcattt cgaccacgga gtttctgttc gatacgattg
    19441 actaccatgt tgatttcatc acttgacaca ggacgtttct gggctgagcg gataatccca
    19501 ttaaagattt tatctctgga gaattgttcc cgtgtgccat cttttttaac aaccactaag
    19561 gttctttctt ctactcgttc gtaggttgta aaacggtgtt ggcactcgtc gcactctcgt
    19621 cttctacgaa tggtgttccc ttcttctgct tggcgactat cgataacact tgacttggta
    19681 gccccacatt ttggacaacg catcctttcc ctccttatcg ttttcttttc attataccat
    19741 tttttaagcg attcccaaaa caattcttct ttttgcttga caagtttttt gttttgttgt
    19801 attatttaat taagacaaaa ggataagaga aaggagacta agatgtcctg gacatttgac
    19861 aacaaaaaac ccatctattt acagattatg gagaaaatca agcttcagat tgtttcccat
    19921 acactggaac ccaatcaaca acttccaacc gtgagggagc tagctagcga ggctggtgtc
    19981 aatcccaata ccatccaaag agccttatca gaccttgaac gagaaggatt tgtctacagc
    20041 aagcgaacaa ctggacgatt tgtgactaag gataaggagc taatcgccca gtcacgcaaa
    20101 caattatcag aagaagaatt ggaacacttc gtttcctcca tgacccattt tggctatgaa
    20161 aaagaagaac taccaggcgt agtcagtgat tatattaaag gagtttaagc ctatgtcatt
    20221 actagtattt gaaaatgtat ccaaatcata tggagcaaca ccagcccttg aaaatgtttc
    20281 tcttgacatt ccagctggaa aaattgtcgg ccttcttggg ccaaacggct caggaaaaac
    20341 aaccctgatt aaactaatta atggcctctt acaaccagat caaggacgtg tcctcatcaa
    20401 cgacatggac ccaagcccag caaccaaggc cgttgtagct tatttgcctg atacgaccta
    20461 tctcaatgag caaatgaagg tcaaagaagc cctaacctac ttcaagacct tctataaaga
    20521 tttcaatctt gaacgcgccc atcatctact tgcagacctg ggcattgatg aaaatagtcg
    20581 tctcaagaaa ctatcaaaag gaaacaaaga aaaggttcaa ctgattttgg ttatgagccg
    20641 tgatgctcgt ctctatgttt tggacgaacc cattggtggg gtggatccag cagcccgtgc
    20701 ttatatcctc aataccatta tcaacaacta ctcaccaact tctaccgttt tgatttctac
    20761 ccacttgatt tctgatatcg agccaatctt ggatgaaatt gtcttcctaa aagacggaaa
    20821 agtcgtccgt caaggaaatg tagatgatat tcgctacgag tcaggtgaat ccattgacca
    20881 actcttccgt caggaattta aggcctaagc aaaggagatt atttatgttt tggaatttag
    20941 ttcgctacga atttaaaaat gttaacaagt ggtatttagc cctctacgca gccgtgctag
    21001 tcctttctgc cctcatcgga atacagacac aaggctttaa aaatctacct taccaagaaa
    21061 gtcaggctac tatgctactt tttctagcta cagtctttgg tggcttgatg cttacacttg
    21121 ggatttcaac cattttcttg attattaaac gcttcaaagg tagtgtctac gaccgacaag
    21181 gctatctgac tttgaccttg ccagtttctg aacaccatat catcacagcc aaactaatcg
    21241 gtgcctttat ctggtcattg attagcaccg ctgtattggc tctaagtgct gttattattc
    21301 tggctttaac agctccagaa tggattcctc tttcttatgt gattacattt gtagaaacac
    21361 atctccctca gatctttctt acaggtatat ccttcctact aaatactatt tcaggaatcc
    21421 tctgcatcta cctggctatt tccattggac agcttttcaa tgaataccgt acagcactcg
    21481 ctgttgcagt ctacattggt atccaaatcg tcattggatt tattgaactt ttcttcaatc
    21541 ttagttctaa tttctatgtc aattcactgg taggactcaa tgaccatttc tatatgggag
    21601 caggtatagc cattgttgaa gaactcatat tcatagctat cttttatctc ggaacctact
    21661 acatcttgag aaataaggtt aatttgcttt aaataatttt tacctagata tgtaacatac
    21721 tcatagaaca aaagagacca ggcaaaaagt ctttacaatt agaaaacgca tagtatcaag
    21781 tgttaaaaat ccttgatatt atgcgttttt tgtgagctga cttatttcct ttcactatat
    21841 cgcaaaatga aataagaacg gaacgatggg attttggaat tcaaatcaat ttataagaat
    21901 gttttagaag taatattatc ctattccaga ttcagttcac tatacaattg agttttcaag
    21961 caacctgttt acataatgtg tacataatta ggttcgtgat tccacccttt tcacctttaa
    22021 aaacctcgct ttcgcaaggc tcttctattt ataagataag gcacgtttaa aggttttcca
    22081 aatccctaaa tcatccgttt gaagaacgag actagcatac atgcgtccga taaatcctgt
    22141 tgctaccacc gcaaaaatca ctgtaatagc aagtgaaatc catgcttccg ctccctccgc
    22201 atagccatta atcgttcgaa acggcataaa gaaggtcgaa ataaagggaa tataagaacc
    22261 aatcttcaag aggagattgt caccagctgc acctagagct gtcactccaa aaaaaccacc
    22321 cataatcaaa atcatcaaag gcgacaaggc tttccctgag tcttcaggac gagaaaccat
    22381 agatcctagg aaggctgcca agactacgta catgaaaaga ctgatcaaaa taaagagcaa
    22441 ggtattcagt gagatagcat ctcccaagtg atccaaaata ccagactgag ccaagaatgg
    22501 caaatcttta aagagcaaaa cggcagccag accacctaca acatagatcc caatatgcgt
    22561 taaaatcact agaaacagag ccatcatccg cgcatagaaa tagtgacttg cccttatgct
    22621 agaaaagacg acttccataa ttttggtgcc tttttcactg gcaacttcct gagctgttac
    22681 acccgcatag gtaatcagaa tcatataaag aaagaatcct aaggcacctg ctgcaattgt
    22741 ttgaataaac tttttatttt ccttggcttc atcaatcttt tctgtgaatt gaattgtctg
    22801 cgctaagcgt ttttcctgct cttgagacaa ggaagcagtt gaacgattaa gctgattttg
    22861 cagttcattg agtgtacctg taacctcaaa tttaattcca ttttcaagcg atgtttcgcc
    22921 atgataaact gcctttagaa cactatcttc ttgatcaatg gtcaaataac cttttaattt
    22981 ttcttcttta attgcttctt tggcacttgc ttcgtcttta tagtcgaagt taacaccatt
    23041 tacattcttc agtccttctg ctacagatgg cactgttgtc actactgcca ctttattatt
    23101 tttagccata gaagaacctt ggagatgccc aattcctaca gagattccta aaaagaggaa
    23161 cggcgaaatc accataaaga agaaactcca tgactcgaca tgtcgaagat aggtttcctt
    23221 gattacaacc cacatatttc tcatacttcc actcctgatt ctagtttaaa gatttcatcg
    23281 atagttggcg cttgttggtc aaatgttgcg atatattgac cttgagtcaa gattgggaag
    23341 agttcccttc cagcgctctc atcctccaaa atcaatttcc aactgccttg tttggtcaag
    23401 ctcacctgtt tgacatgagg aagattttcc aattcttcct tgcttcgttc acttgaaaca
    23461 aagagacgcg ttttcccgta ttgattgcgg acatcctgaa ctggtccgtg caagaccaca
    23521 cggccatctc gaatcatcag aatatcgtca caaagttcct caacattggt catgacatgg
    23581 tcagaaaaga taatggttgc tccgcgctct ttttcctgaa aaatgacttg tttgagcaat
    23641 tctgtattaa ctgggtccaa tccactaaaa ggctcatcca agataatcag gtctggttca
    23701 tgaatcagag taataatgag ctgaatcttc tgctgatttc cttttgacag actcttgatt
    23761 ttatctgtca gctttccttt tacttccaac ctcttcatcc attgagggag tttttctttg
    23821 acttctttgg catccatgcc ttttagagtc gccaagtagc gaacttgttc aagaactgtc
    23881 aatttaggca tgagactgcg ttcttcaggc agataaccaa tccgagcata gatctcctga
    23941 cgaatatcct gaccatccag accgatttct ccctgatatt ctaggaattt taaaatacta
    24001 tggaaaatcg ttgtttttcc agcaccattt tttccgacta gtcccaaaat acgacctggt
    24061 cgcgcttgaa agtcaatacc aaacaaaact tgcttggatc caaaactttt ctctagactt
    24121 cttacttcta gcatctttca cctccgaaat ttcttgcact cattatactc ctttttgata
    24181 gcctttacaa tgttttttgt ccatttttag aagactattg ctgtgtaaaa tatggcctgg
    24241 agcactttta tactcaatga aaatcaaaga gcaaactagg aagctagccg tagactgctc
    24301 aaagtacagc tttgaggttg cagataaaac tgacgaagtc agctcaaaac actgttttga
    24361 ggttgtggat agaactgacg aagtcagtaa ctatacgtac ggcaaggcga agctgacgtg
    24421 gtttgaagag attttcgaag agtattagtg ataaatccat tatacagcag caaacttaat
    24481 ttataccttc cgctcctcaa ctgtctattt ttaatcctga attgttattt gagtaactcc
    24541 tttttcctcg taaagttttc ttcctctaaa acttctggaa aaaggctaat agtttcagac
    24601 aacattttta taagaaacaa gttcatctgt catttcaaga aggagtaatc ctttatctac
    24661 taatggacgg aacagaattc aaccgcttgt ccgatatgtt ttctaaggat tatatagtaa
    24721 aatgaaataa gaacaggaca aattgatcag gacagtcaaa ttgatttcta acaatgtttt
    24781 agaagtagat gtatactatt ctagtttcaa tctgctatat ctattatgca cacccctata
    24841 ggatctaatg aaaatcacaa caggctcatt catagatagt tacctcagga aactaagaaa
    24901 acgactacaa aggaagtcgc attcatcgaa aagtggatta acaactatcc taaaaaatgc
    24961 ttgaactaca agtcccccag agaagacttc tggatgacta acttgaactt gaaatttagc
    25021 aataattaat tcaccatcta actatattta gtaattattt cagaactgat taatattaaa
    25081 attaactaac aattcaaagg attcatacta gccataaatt acgtccatca gagagagact
    25141 cttactactt ttagatttta gtctttctag cttcagaata catctaaact ttagggaaaa
    25201 tgactattcg aaagcgcgaa tgcctcaaaa ttatctcaga taagctattc gaaacttaga
    25261 atgcttttaa atttatggaa ttgcgattat tcgaaaccta gaatgcatat aacctttagt
    25321 tgacagacct attctaagtc tcgaagggct atttactttc tattccttat caaaaaagac
    25381 tcattccccc tttctcctcc aaaatatggt atagtagaaa tatactatct atgaggagtt
    25441 tacatgtcac aggataaaca aatgaaagct gtttctcccc ttttgcagcg agttatcaat
    25501 atctcatcga ttgtcggtgg ggttgggagt ttgattttct gtatttgggc ttatcaggct
    25561 gggattttac aatccaagga aaccctctct gcctttatcc agcaggcagg catctggggt
    25621 ccacctctct ttatcttttt acagatttta cagactgtcg tccctatcat tccaggggcc
    25681 ttgacctcgg tggctggggt ctttatctac gggcacatca tcgggactat ctacaactat
    25741 atcggcatcg tgattggctg tgccattatc ttttatctag tgcgcctata cggagctgcc
    25801 tttgtccagt ctgtcgtcag caagcgcacc tacgacaagt acatcggctg gctagataag
    25861 ggcaatcgtt ttgaccgctt ctttattttt atgatgattt ggcccattag cccagctgac
    25921 tttctctgta tgctggctgc cctgaccaag atgagtttca agcgctacat gaccatcatc
    25981 attctgacca aaccctttac cctcgtggtt tatacctacg gtctgaccta tattattgac
    26041 tttttctggc aaatgctttg acacgtaaaa aatccgtttg gtttcccaag tggattttta
    26101 aagcgtagat taactatagc ttgatactaa atatactttg gtatggaaat catgcatatt
    26161 tttcgatagt gaagcgagga cttacctagc ctttccaccg tgatagaaac acctgaaatc
    26221 taatggtttc aggtgttcgg aaactttgag cctagtgtct caaagtttag gtatggaatt
    26281 tcgaagaaag tcgctaccgt ccgtaatcac ttaaggaaag gctcaaaaat attgttttca
    26341 accacaaaat ccgtttggtt tcccaagcgg attttgtgct ttattttgaa acttcttttg
    26401 caagaacaaa gttcccaagt gtggcagaac catttcctgc gactgctggc gtcacgatat
    26461 agtcacgcac atctggtact ggtaggtaac cattaagaag agatgtaaat ttctcacgga
    26521 cacggtccag catatgttgt tgagccatga cccctccacc aaagacaatc acgtctgggc
    26581 ggaaagtcac tgtcgcatta accgcagctt gagcgatata gtaggcttga acatcccaaa
    26641 cagggttgtt gagttcaata gtttccccac gtacacctgt acgagcttcc aaacttggac
    26701 cagctgcata gccttccaga catcccttat ggaaaggaca aacaccctta aactcttttt
    26761 caatatccat tgggtgtcta gcaacataat aatgacccat ttcagggtga cccacaccac
    26821 cgataaactc accacgttgg atgacgcctg caccgatacc tgtaccgatt gtgtagtaaa
    26881 ccaagttttc gatacgacca ccagcattgt tacgggcaac cacttcaccg taagcagagc
    26941 tgtttacgtc tgttgtgaag tacattggca cgtttagggc gcgacgaagg gcaccaagca
    27001 agtccacatt tgcccagttt ggttttggag tcgtcgtgat aaagccataa gtttttgagt
    27061 ttttgtcaat atcaatcggc ccaaatgaac caactgcaag accagcaagg ttatcgaatt
    27121 ttgagaagaa ctcaatggtt ttatcgattg tttcgattgg agttgttgtt ggaaattgtg
    27181 ttttttctac aacgttaaag ttttcatcac cgacagcaca gacaaacttt gtaccgccag
    27241 cttccaagct tccatataat tttgtcatga taaacctctt gtttttattt tctttattat
    27301 agcatacttc gaaagtctaa atgtctctat tttttagatt ttcctctgta aatcttacta
    27361 tctaataaaa acgaacaaac atgtcatttg ttcgttttca cattagagag gattgattag
    27421 attttcactt cgatcacagc atcccccttc gcaactgaac ctgttgcgac tggagctact
    27481 gaagcgtagt cagctgtatt tgtaacgata accattgttg tatcatcaag tccagctgta
    27541 gcgattttat ttgagtcaaa tgttccaaga acatcgccaa ctttcacctt atcaccttga
    27601 gcaacttttg cttcaaaacc gtcaccgttc atagatacag tgtcaatacc aacatgaatc
    27661 aaaacttcag caccatttct tgttttcaaa ccaaaagcgt gccctgttgg aaaggcaatt
    27721 gaaacttcag catcagctgg tgcatagacc acgccttggc ttggtttcac agcgatacct
    27781 tgtcccatag ctccacttga gaagactggg tcattgacat cagcaagagc gacaacatca
    27841 ccgacgatag gagttacaag tgtttcattt tgaagagctg ctggcgcaac ttcttctttt
    27901 tcttcagcca cttcagcttg ttttgcagct gcagttgcgt ctacttcatc ttcgtaacca
    27961 aacatgtaag taagagcaaa accaagggca aatgatacag ctaccataag aaggtattgt
    28021 ggaagttgtc cgttaccaac ataaagcatt gtaccaggga tgatggtgat accattacca
    28081 gtaccagcaa gtccaaggat agaagccaat ccaccaccga ttgcaccagc aatcaatgaa
    28141 aggaagaatg gtttacggaa gcgcaagttc accccgaaga tagcaggctc tgtaatacct
    28201 aggaaggcag aaagagcagc cgggaaagca agtgttttca gttttggatt ttttgtttta
    28261 acaccaaccg caacagtagc agcaccttga gctgtcatag cagctgtgat gatagcgttg
    28321 aatgggttag catggtcagc agcaagtaat tgcacttcaa gcaagttgaa gatgtggtgc
    28381 acacctgaca cgacgatcaa ttggtgaacc ccaccaatca agaaaccacc aagaccaaat
    28441 ggcatgctaa gaatcgcttt tgtagcaata aggatgtagt tttcaacaac gtggaaaact
    28501 ggtccaatga caaagagtcc aaggatagac atgaccaaaa gtgtcacgaa tggtgttacc
    28561 aagaggtcaa tgacatctgg aacaaccttg cggacagctt tttcaaattt agctccgaca
    28621 accccgatga tgaaggctgg aagaacggaa ccttgcaaac caacaacagg gatgaaacca
    28681 aagaagttca tcgctgttac ttcaccacct tgagcaactg cccaagcgtt tggaagtgag
    28741 ccagagacaa gcatcatacc aagaacgata ccaacggcag gatttccacc aaatacacgg
    28801 aaggttgacc acacaaccaa acctggcaag atgatgaagg ctgtatctgt caagatttgt
    28861 gtgtaagttg caaagtcacc tggaagtggc atttcaagag cgttgaaaag accacgcaca
    28921 cccatgaaga gacctgtcgc tacgataact gggatgattg gaacgaaaac atcaccaaaa
    28981 gtacggatag cacgttggaa ccagttccct tgtttagcaa cttctgcttt catgtcatcc
    29041 ttagatgatg ttggtaatcc aagtacaaca acttcatcgt acattttgtt aactgtacct
    29101 gtaccaaaga taatttggta ttgccctgag ttaaagaaag caccttgaac tttttccaag
    29161 ttctcaatca cttctttatt gattttctct tcatctttga ccatgacacg tagacgtgtc
    29221 gcacagtggg caacactatt gacattttca cgtccgccca aggcatcgat gacttttttt
    29281 gcaatttcct gattgttcat ttgcaaaaat ctccttatat aacattttgt tcttgtttga
    29341 aagcgatttt attcgccctt acgactatta ttttatcatg ttttaaaaat atgtcaagcg
    29401 ttttgcagaa aaaatattct atctacttag tgggcaagct ttagattgat tgtttttgac
    29461 tgtttcatca ggaatttctt gaaaaataag tttgaaacct tggttaaaaa gtcttttatt
    29521 tcattatctt tcacattttc gtaaaaattt gattgatttc ctgtatttat tttatgataa
    29581 tcgtttgaca gttttttctc ttttttaaca taaagggctt gcatttttta tcgaaaacag
    29641 ttactattaa aagtgataag atttttggag gaatgaatgg aatggacaac tgagcgtcgt
    29701 tacagacttt atcaagattg gacgcaagaa gaaattcaac atataaagga aaatatggca
    29761 caatctccat ggcatactca ttaccatgtt gagccaaaaa caggacttct caacgaccca
    29821 aatggctttt cttactttga tggcaagtgg atcctctttt accagaattt tccttttggt
    29881 gcagcccacg gtttaaaatc ttgggcacag ctagaaagtg atgatttggt tcactttaga
    29941 gaaactggag tcaaagtttt gccagatact ccattagata gtcacggtgc ctactctggt
    30001 tctgccatgc aatttggcga taacttattc ctattttata caggaaatgt tcgcgatgaa
    30061 aactggatcc gtcacccata ccagatcggt gctttgatgg acaaggaggg taagattaca
    30121 aagattgaca agatcttgat tgaccagcca gcagactcta ctgaccactt ccgcgatcca
    30181 caaattttta actttaaagg tcaatactat gctgtcgtcg gtggacagga cttggagaaa
    30241 aaaggcttcg tccgtctcta caaggctgtc aataacgact acacaaactg gcaagcagtt
    30301 ggcgaccttg actttgctaa cgaccgtact gcctacatga tggaatgtcc taatctggtc
    30361 tttgtagagg aacagcctgt ccttctctac tgtccacaag gattggataa gaaagttcta
    30421 gactacgata atatctttcc aaatatgtat aagattgggg cttcctttga ccctaaaaat
    30481 gccaaaatgg tagatgtgtc tcaacttcaa aacatggatt acggtttcga agcctatgca
    30541 actcaagcct tcaacgctcc tgatgggcgt gctctagcag ttagctggct tggtttgcca
    30601 gatgtttctt acccatctga ccgttttgac caccaaggaa ccttctcttt ggtcaaggaa
    30661 ctcactatca aagacgacaa gctctaccag tatccagtcg ctgctattaa ggaccttcgt
    30721 acttctgaag aagccttctc aaaccgttcc caaaccaaga acacttacga acttgaactc
    30781 aacttggaag ctaatagcca gagcgagatt gtcttacttg ctgataaaga aggtaaggga
    30841 ctttcaatca actttgacct tgtaaacggt caagtgacag tggatcgtag ccaggctgga
    30901 gagcagtatg cccaagaatt tgggataact cgttcttgcc ctatcgagaa tcaggctact
    30961 actgctacaa tcttcatcga taactctgtc tttgaaattt tcatcaataa aggagaaaaa
    31021 gtattttctg gtcgtgtctt cccacatgcg gaccaaaatg gtatcctgat caaatctgga
    31081 aacccaactg gaacttacta tgaattagat tatggtcgca aaactaactg atgtcgccaa
    31141 acttgcaggc gtcagtccta ctaccgtttc tcgggttatc aataaaaaag gatatctatc
    31201 tgagaaaacc atccaaaaag tcaatgaagc catgcgagaa ttgggctata aacccaacaa
    31261 cctggctcgt agtctgcaag gaaaaccagc taagttaatc ggcttgattt tccccaatat
    31321 ttccaatgtt ttctatgcag aattgattga taaattggaa caccaactct tcaaaaatgg
    31381 ttacaagacc atcatctgca acagtgaaca tgattctgag aaggaacgtg aatacatcga
    31441 aatgttggaa gccaatcagg tggacggcat catttctggt agtcacaacc taggaatcga
    31501 agactacaat cgtgtgacag cgccgattat ttcctttgac cgaaacctat cgccagacat
    31561 ccctgtcgtc tcctctgaca actatgctgg tggggttctt gctgcccaaa ccttggtcaa
    31621 gacaggtgcc cagtctatca tcatgattac agggaatgac aattccaatt cgccaaccgg
    31681 actgcgccac gctggttttg catccgtact cccaaaagct cctattatca atgtttccag
    31741 tgacttttct cccgtcagaa aagaaatgga aatcaagaat atcttgaccc gggaaaaacc
    31801 agatgccatt tttgcttcgg atgatttgac agctattctg gtcattaaaa tcgctcaaga
    31861 attgggcatt tctgtcccaa aagagctcaa ggtcatcggc tatgatggga cctactttat
    31921 cgaaaattac taccctcaat tggctactat caagcaacct ttggaagaga ttgcttgtct
    31981 cactattgat cttctcttgc aaaagattga aggcaaggaa gtcgccacaa ctggttactt
    32041 cttaccagtt acgctattac caggaaaaag tatttaaaca caagaaaact cagaccgatt
    32101 cgtctgagtt tttatgatct taaattttcg agatagcgct gggctgtctc taggttaaag
    32161 gttttatctg agatgaggcg ctctactagg ggagcaactt cagattcact agccccagct
    32221 aggagagcta gggatttggc ctgtagtttc atgtggcctt gctggatgcc cgtacttacc
    32281 aaggctttga gggctgcaaa attttgagca agaccgatgg acacgataat ctgggctaat
    32341 tctctggcag aaggatttcc tagtagatca tgactgagag ctacacgtgg gttgaggccg
    32401 atagagccac ccttagtcgc tacaggcatg ggcagggtca tctcaccgac caattcttct
    32461 ctttcaaggt ccagcgtcca gcagctaaga ccttgatagc gtccatctcg actggcaaag
    32521 gcatgggccc cagcttcgat ggcacgccag tcattaccag tggcaatcaa aatcgcatca
    32581 ataccattaa aaattccttt attatgagta gcagctcggt aaggatcagc ctgcgcaaac
    32641 tgactagcca acgcaatttt ctccgcaatc tctcgtcctt gatccttttg gcggctcaag
    32701 tagcgaaagg cgatgcgaca gcttgcagtc accagagaat cagtcgcgta gttggacagg
    32761 attcccatga gactctgtcc ctgactgagt tcttctaaga ctggtttcaa ggcttccagc
    32821 atggtgttga gcatattggc acccatggct tcctgggtat cgacatgaat ataaacaacg
    32881 agaaagtctg gttcgccttt tatctgctcg acatgcagat cacgcgcccc acccccacgt
    32941 ttaacgatag aaggataggc ttgattggca agctccaaga gctccgcttt cttgctggca
    33001 atcttctctt gcgctagttt aggattagca acttgataaa gggctacctg cccaatcatc
    33061 tggcgctgat ggacttgtgc agtaaaacca cctgcacgct tgatgatttt gctggcatag
    33121 ctggccgccg caaccacaga gggttcttct gtcacatagg gaacggtgta ttcctgaccg
    33181 ttgacaagta cctccggaac cagcgaataa ggcagagaaa aagttcccac cacattctca
    33241 ctcagctggt ctgccacagt cacactcatc tgttcatcct tctccagact agcttgtctc
    33301 tcaggactaa ggagcgcctg agcttttagc agctcgaggc gctcttggta tgatttttta
    33361 gaaaatccat tccaacttat cttcattatt tttcaacctt gctataacgg cgttggtggt
    33421 caagaatttc aaccaaggca aaatcttgat tttcatagcc agcaaactgg gcagagtttg
    33481 tttcatccaa gtttacttcc tcaaaaaaga ccttttcata gtctgcaacg gatagggcag
    33541 ttcgttggtt gagcttgttc aagcggtctt tatccaaata agcttcatat ccttcaacca
    33601 attcaccact gaagaactca gccacagctc cacttccgta actataaagg gcgattttat
    33661 ccccagcttt caagctatct gtattttcca agagagacaa aagtccaagg aaaagtgaac
    33721 ctgtgtagat attccccacc ttttgactgt agagaataga ctggtcaaaa tgcttttgta
    33781 agaggtcttt tttctcttga ggcaggctct tatccatgat ttttttcaag ccttttagcg
    33841 ctaatttagg ataaggcaag tggaaacaaa cagccgcaaa atcatccaaa gtaagctggt
    33901 agcgtttttg atattcaagc caagtcgttt tcaaactatc caagtattgt tgggtagaat
    33961 agacaccatt tacataagga gttgtcgagt aatttggtcg ccagaaatcc atgatgtcac
    34021 gggtctgagc tacattgtca ttattaaagg ccatcatgcg tggattttgt gtaatcaaca
    34081 tagctacact tccagcacct tgagttggtt ctcccggagt ttcaatacca tatttggcaa
    34141 tatcactggc aatgaccaag accttggact ccggagaatt ttccacatgc aatttggcat
    34201 aatggagggc agctgtcgcc ccatagcagg cttctttaat ctcgaaacta cgagcaaagg
    34261 gctggatgcc cagcaagcca tgcacaaaga cggccgcagc cttactctgg tcaattcctg
    34321 actcggtagc cacaatgacc atgtcaactt cttgtctttc ttgctcagtt aaaatagagt
    34381 cactagcact ggccgccaag gtcacgatgt cctcagttag gggcgcaata ctcaattcct
    34441 tgagtaagag tcctttactt aatttttcag ggtcaattcc cctcgcttct gctaagtctt
    34501 gtaatttcaa gacatattga ctggtcgcaa aaccaatctt atcaataccg attgtcatat
    34561 ttacctctgt tttatcattc atgtaaaaaa tcgttctata ctattttatc acaaatggca
    34621 gtaaaagaga gaaaaaagac ttgattcacc aaatcaagcc tcttattggt catcatttta
    34681 aagaatgatt agttgctaga gagttcaccg atataagtag ctttataagc tccattcaca
    34741 gttatcagct cctggaggat caaatttcct gagtaaatcc ttcccatctc atctacaaat
    34801 ttttgataaa actgactgat cggaatttct ctgacatcct tatcaaatgt cttatcaagt
    34861 gttttactaa ccttctcagc aatcaattga tgctcttgcc atccactttg aaactctgag
    34921 cccgaactag aaaccatgac tgggataaac aacaaggtca gtagatttac agacaataag
    34981 gaaagta
//
LOCUS       DBERYS010000019        34443 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.019, whole genome shotgun sequence.
ACCESSION   DBERYS010000019 DBERYS010000000
VERSION     DBERYS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.019"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(297..1283)
                     /locus_tag="ACL8LA_001043"
     CDS             complement(297..1283)
                     /locus_tag="ACL8LA_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1812815.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1293..2093)
                     /locus_tag="ACL8LA_001044"
     CDS             complement(1293..2093)
                     /locus_tag="ACL8LA_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGS1812816.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2346..3188)
                     /locus_tag="ACL8LA_001045"
     CDS             complement(2346..3188)
                     /locus_tag="ACL8LA_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGS1812817.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3190..4482)
                     /locus_tag="ACL8LA_001046"
     CDS             complement(3190..4482)
                     /locus_tag="ACL8LA_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGS1812818.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4593..5864)
                     /locus_tag="ACL8LA_001047"
     CDS             complement(4593..5864)
                     /locus_tag="ACL8LA_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1812819.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6399..7931
                     /gene="malQ"
                     /locus_tag="ACL8LA_001048"
                     /pseudo
     CDS             6399..7931
                     /gene="malQ"
                     /locus_tag="ACL8LA_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            7957..10215
                     /locus_tag="ACL8LA_001049"
     CDS             7957..10215
                     /locus_tag="ACL8LA_001049"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HGS1812820.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11259..11831)
                     /locus_tag="ACL8LA_001050"
     CDS             complement(11259..11831)
                     /locus_tag="ACL8LA_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812821.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            11908..12114
                     /locus_tag="ACL8LA_001051"
     CDS             11908..12114
                     /locus_tag="ACL8LA_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812822.1"
                     /translation="MLKKYFSKYKWTDLFWILFVILTCLYIGNHDLFTLNHQEFSFRG
                     SVWGLVLALYHLLFIDKFVISNRK"
     gene            complement(12453..13301)
                     /locus_tag="ACL8LA_001052"
     CDS             complement(12453..13301)
                     /locus_tag="ACL8LA_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGS1812823.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13453..13731
                     /locus_tag="ACL8LA_001053"
     CDS             13453..13731
                     /locus_tag="ACL8LA_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGS1812824.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13733..15796
                     /locus_tag="ACL8LA_001054"
     CDS             13733..15796
                     /locus_tag="ACL8LA_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS1812825.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLVRTKELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15874..17130)
                     /gene="tyrS"
                     /locus_tag="ACL8LA_001055"
     CDS             complement(15874..17130)
                     /gene="tyrS"
                     /locus_tag="ACL8LA_001055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGS1812826.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17272..19737
                     /gene="pbp1b"
                     /locus_tag="ACL8LA_001056"
     CDS             17272..19737
                     /gene="pbp1b"
                     /locus_tag="ACL8LA_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGS1812827.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19802..20713
                     /locus_tag="ACL8LA_001057"
     CDS             19802..20713
                     /locus_tag="ACL8LA_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1812828.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20943..21641
                     /gene="dapD"
                     /locus_tag="ACL8LA_001058"
     CDS             20943..21641
                     /gene="dapD"
                     /locus_tag="ACL8LA_001058"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGS1812829.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21709..22839
                     /locus_tag="ACL8LA_001059"
     CDS             21709..22839
                     /locus_tag="ACL8LA_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGS1812830.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22851..23390
                     /locus_tag="ACL8LA_001060"
     CDS             22851..23390
                     /locus_tag="ACL8LA_001060"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGS1812831.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23374..24051
                     /locus_tag="ACL8LA_001061"
     CDS             23374..24051
                     /locus_tag="ACL8LA_001061"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGS1812832.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(24123..25022)
                     /gene="galU"
                     /locus_tag="ACL8LA_001062"
     CDS             complement(24123..25022)
                     /gene="galU"
                     /locus_tag="ACL8LA_001062"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGS1812833.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(25044..26060)
                     /locus_tag="ACL8LA_001063"
     CDS             complement(25044..26060)
                     /locus_tag="ACL8LA_001063"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS1812834.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26218..26712
                     /locus_tag="ACL8LA_001064"
     CDS             26218..26712
                     /locus_tag="ACL8LA_001064"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1812835.1"
                     /translation="MIQYMLIIEVNNSGSSCRLREFGEKIKRLRLAKKISRSEFCGDE
                     SELSIRQLIRIENGESRPTLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRT
                     PTYEDETIAQKKESVFDKIFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEI
                     LSFW"
     gene            complement(26835..27257)
                     /locus_tag="ACL8LA_001065"
                     /pseudo
     CDS             complement(26835..27257)
                     /locus_tag="ACL8LA_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27319..27969)
                     /gene="phoU"
                     /locus_tag="ACL8LA_001066"
     CDS             complement(27319..27969)
                     /gene="phoU"
                     /locus_tag="ACL8LA_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS1812836.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27984..28736)
                     /gene="pstB"
                     /locus_tag="ACL8LA_001067"
     CDS             complement(27984..28736)
                     /gene="pstB"
                     /locus_tag="ACL8LA_001067"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1812837.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28738..29553)
                     /gene="pstA"
                     /locus_tag="ACL8LA_001068"
     CDS             complement(28738..29553)
                     /gene="pstA"
                     /locus_tag="ACL8LA_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS1812838.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29546..30409)
                     /gene="pstC"
                     /locus_tag="ACL8LA_001069"
     CDS             complement(29546..30409)
                     /gene="pstC"
                     /locus_tag="ACL8LA_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS1812839.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30527..31402)
                     /locus_tag="ACL8LA_001070"
     CDS             complement(30527..31402)
                     /locus_tag="ACL8LA_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGS1812840.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31569..32900)
                     /gene="pnpS"
                     /locus_tag="ACL8LA_001071"
     CDS             complement(31569..32900)
                     /gene="pnpS"
                     /locus_tag="ACL8LA_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGS1812841.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32901..33608)
                     /locus_tag="ACL8LA_001072"
     CDS             complement(32901..33608)
                     /locus_tag="ACL8LA_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1812842.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33692..34168)
                     /locus_tag="ACL8LA_001073"
     CDS             complement(33692..34168)
                     /locus_tag="ACL8LA_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGS1812843.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            34175..34443
                     /locus_tag="ACL8LA_001074"
                     /pseudo
     CDS             34175..34443
                     /locus_tag="ACL8LA_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT        10163 a         6516 c         7365 g        10399 t
ORIGIN      
        1 gcaacctcaa aaccatgttt tgagctgact tcgtcagttc tatctgcaac ctcaaaacca
       61 tgttttgagc tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagcaacct
      121 gcggctagct tcctagtttg cactttgatt ttcattgagt ataaaaagat cttgggaaaa
      181 gactcaattt cagtagaaaa ggaagtaaat cttcccacaa taaaacgcat agtatcaagt
      241 tttttcaata cctgatagta tgcgtttttg cttttaacga ctctttgccc agtctcttat
      301 ttttcgataa ttttgtgggc gatcaattga cggtaacaaa tttgtttatt gtttttatta
      361 tcattaataa tctggagaat agtttcaagg gaaacacgac caagctctaa actattgata
      421 tcgacatagg ctgccaagtt gagcttggga ttaaccgagt caaagctgag aacagggaca
      481 tccagctggt gtttggcaat atagttacaa acaccttcag ctaggaggct atcggttgtg
      541 atgatagcat caatttgtgg atcgtgcttg aataatcgct tgctaaattt atagcccttt
      601 tcttccagaa actcgtcggc aaagtagatg cgattgttgt cagtggtaag tttgtaatgt
      661 ttaagcgcct gttcatagcc tgttaaacgg tctttggtca cgaagagctt tttacttcct
      721 ccgataaagg caatgcgttt gcagcctttt ttgatgaaat attcagtcgc atcaaaacca
      781 gcttgaacat tgtcgttgtc gacaagtggg atgaaaggag atagagattt acctaggata
      841 aggaagggga actgttcttc tgcgacgagt tttacgagag ggtcttcttc ttgggcatag
      901 agaaagatta gcccatctac acgcttgccg tagaccattt gtgaaatagc gttgagacgc
      961 tccttctcat ctttccctgt tgctatctga atggcatagt ggttttcaga tgcgacttga
     1021 gagatgccac gtagaaccga tggaaagaaa ggattctggt agaaggcgtc tgagtcatca
     1081 ggaagaacta atccgataac ctgagtatag ctgcttacca agctacgagc gttgaggttt
     1141 gggtggtagt tgagttcctt catagccttg cgaacgcgtt tttttgtttc gtcgctaatg
     1201 gttgatttat tttgaataac acgggttacg gttgaaggcg aaacaccagc agccttggcc
     1261 acgtctttaa tcgtaacggg cataaaaatc tcctatttat ggtaatttgg atcacggaaa
     1321 tgtgttttat aaaagatagt gaccagatac agaacaaaaa gaatattttg tacagtaatc
     1381 agggttgtca tattttggcc aaataatccc aaaataagtg taatcagagt cggcaatcct
     1441 aaacagttca agataaagtg atagcactct ttaaaggttc taaatgaaaa gaggcgtgat
     1501 ttcttagtga tataaaggag aagactagct cctagagaga cgataaagaa attcaaacca
     1561 aagaggaagc tcgcaccgag aactaggaag aggctgatat agacacgatt ttgttggtac
     1621 cagtctttag aaattgcttg ggtcaagctg tctttgcttt tgaaactctc agtctgaatg
     1681 gctcggtaag agatgcgggt cagttccttg ctttccttgc tgatgactag ctcatttgta
     1741 tcgaaatgca gttgcaagtt cttaggtaat tccttgattt gacttggacc aatcacaaca
     1801 gaagaggctt gactaactgt tccagtataa gttaatgtgc catcgacaat tgtagcatgt
     1861 tcagagagat cctggacaac ctcatttgtc agaggttcat agacattatc gataaaagtt
     1921 tctagcggat aggtctcctg ggagctgttt tggatagcaa tgggtaccat agacaagctg
     1981 ataaggaaga tactggtaaa gagaagttga aaccagttga gcccgaaacg tttggacagg
     2041 ggcttacgaa accccaaaat acttgaaaaa aaggaaaatg gatatggaag cataggtatc
     2101 tttctaaaag gtgttttcta tataagtatt attatataga gaaatgcgct ttatttcaag
     2161 tctaggagaa aaattgcttg ctacaaggat attatttaca gtgacaattg ctaataatca
     2221 atgaaaatca aagtgcaaac tagccacagg ttgctcaaag cacaactttg aggttggaga
     2281 taaagctgac gtggtttgaa gagattttcg aagagtataa aatgaaaaag ggtggcgggg
     2341 ataaattatc ccttgtcgcc accacttgta agtcctgaaa caaagttctt ttgtaggaag
     2401 aagaagagaa tacagattgg aagggcgatg aggatagcac ctgctgagaa gtaggcaatc
     2461 ttcaagtttt tcgcattgtt aacgaaggtt tggagaccta cggcaacagt aaagtattct
     2521 ttctcacgaa gcaagaaact agagaggatg tagtccccga aaggtcccat gaaggcccag
     2581 agagcttgta cggcaaccat tgggcgaaca agtggaagaa caatttgcca gaagcggcgg
     2641 aagtgtcctg caccgtctag ttttgcagat tcgtctaaag acattggcac tgtatcgaag
     2701 tagcctttca tgagccaagc attcatcggg ataccaccac caacgtagag gaagatgagg
     2761 aaccagttgt ggttaagggc gttcaacata agcgccataa cgaagaaggc tgtcaaagcg
     2821 gccatagttg gcaccatttg gatgatcaag aagaagacca aactttgttt acgagccaag
     2881 aagttgtaac ggctgtaagc ataaccagca agtacgatga tacttgtttg aacagccatg
     2941 gtaattaagg cgataatcaa agtgttgagg taccaagtac cgtacaaggt ttcagtgaag
     3001 aggcctttaa agttatcaaa attgaggtcg atattagtat ctagtttaaa ggctgagacg
     3061 ttacctgctt taaaggctga cataatggta atcaacagtg gatagataat tacaattgat
     3121 agaccaatca ggtaaaggta agtaaggctt tgagtcagtc tacgtttgag tttaattgag
     3181 ttattcatct tagacgtcct ccatatcaaa tgcgtgtagt ttcttgaatg cgatcataga
     3241 gattgagatg acaatgatag agataatcaa ggtaacagct gccgccattg agtattgagg
     3301 agatgtacct gttgtcaaac ggtagatcca tgagatcaag atatcggttg aaccagctcc
     3361 acctccgaca ctaccaggtc ctccaccatt gaagaggtac atgatagaga agttgttaaa
     3421 gttgaaggtg tattggctaa tcaaagtagg tgccgcaaca gccaaaatca ttgggaaagt
     3481 gatgttgcgg aatttttgcc aagcgttggc accgtcaata taagctgctt cgtaaaggtc
     3541 gttaggaata gattgcaaga tacccaaggt cagaacgtag atgtatggga atccgagcca
     3601 accttgcatc ataatcaagg caatcttagt ccaagttggg tctgttttcc aaggaataag
     3661 agctccatca aggaaaggaa ggaatttagc caagattggc aatacttgag tgttgatagc
     3721 accgacacta tcgttaaaca tgtttgagaa tgtcaagata gtgatgaagg ctgggacagc
     3781 ccaaggaaga aggaaaataa caccaaagat acgttttcct ttgataaatg gttggttggc
     3841 aatgatagct gtgaagatac caattacgat ttgtaaagta gaagctgcca aagcccaaat
     3901 gatagtccaa gaaagaacag aaccaaaggc agaacggaag gtactcaagc tccaaatgtt
     3961 tgtaaagttg gtcaaaccaa cccagtccaa caacttgttt ggtggcaagt gttggaagtc
     4021 gtagttggta aaggcgatca tcaaggttac gataactggg aagataatcg cgaaggtcat
     4081 ggcaacataa gatggaatga tcaagaggta agggaagcca ttttcataaa tccctttgat
     4141 catgtctttg agtgtgcgtg gaactggaat tccattgtta atgcgttttg caatcgtatg
     4201 tgcatctttg atatttgaga aataaaagag tacataaacg attacaaaga ttagatggaa
     4261 ggcaccacga atcagcataa agagggaatt atcacgacct ggtttgtcac caagagtgat
     4321 gaggttgctc aattcagggg ttgcaagtgc taggaagtaa aggacaaata cgatggttac
     4381 accaaggaag ataaaacctt tggctttttg tttattgtaa atctgtccta acccaggaat
     4441 gatagacagc agggctgctt tactaggttg ttgcttttcc atattaactc ctttcatagg
     4501 atactacttt tttataagaa agctaaatta tttgtgtctc tattgataaa ttcaaagggg
     4561 gatttgattt ccacccccct tgaacaaatt ttttattcac caaatttttg tttgattgtt
     4621 tctttgatca atgttacagc atcgttagca gctgttttag catctttttg accacttaca
     4681 gcatcaaaga gcatattttt cgctggatcc caaactgcag acatttgaga gatgtttggc
     4741 agtggttgag tgttcttgaa ctgtttgata acagctgttg tcaactcatc gtttttacct
     4801 tcagcgtatg aacgagcctc agtattagct gggatttcgt tagtcttatc atataatact
     4861 ttttgttgtt cagttgcaac aaggaagtct acaaattttt gagaagcttc aaggttctta
     4921 acggcttgag gaatgaccca agctttacca ccaccgaatg cagcatattc ttttccattt
     4981 ggaagagttg ggatagttgc aactccgtag tttactttag catctttaaa ggcttgagct
     5041 ttccaaggtc cgtcgatgat agcagctgtt ttaccttctt ggaattgagt ttggattaag
     5101 tttccagcac cttctgtatc ttgcatacct ttaggccatt tttcgtacca agatttagcg
     5161 tagttgatac ctacgataga accgtcgttt gcaagaccga tgtctttagc gtctttaccg
     5221 ttttggccaa agacgtaagc accgttaccg gcaagaagtc catatgtata gtagaagttt
     5281 gtccagtcag ctaggaaggc agtagtttta ccatcttcac cagcgaatgc gtatttgcta
     5341 tctttagcaa ggttttccaa gtcagcaaat gtttttggag catctttcac caagtctttg
     5401 ttgtagtaca taacaagtga ctcgataacg gcaggagcac cgtaaacttt accattagca
     5461 gctgttacaa gagatttagt tgtgtcgtct gttttagcac catcgctcaa tttcacttct
     5521 gaaagttgtc cgtcagaacc aaggctacct acacggtcgt atggagccat cataacatca
     5581 gggacattac cagattggtt gtcaagagaa agtttatcaa gacctcctag agcatcacca
     5641 gttttaagag tgacttttac tccagcttct ttttcataag ctttagcaac ctcttcaata
     5701 tagctcttat atccctcgtc tacatataca gtgagttctt tgacttcaga tgaaccagaa
     5761 tcagcaggct tatcagcagt tttgcttccg caagctacca aaagcaagct agcaagtgta
     5821 acagttccaa gcaccgcagt gctcttcata aatttagatg acatagtgta ttcctcccaa
     5881 agaatagcaa gttttattga taaggaaacg caaacgtttt cctttatgag cttagtatag
     5941 cacaaataga aaacggttgc aagtattttt tgtaaaaatt ttaaaaaatt tttaagcttg
     6001 atttgatagc ataattttgc attttaatag aagaaaagta tgaaatagat aagaatacag
     6061 tatttttaat aaaaatatat ggaatctgtc taaagaggga aaccaggagg ggctacctcc
     6121 ctggttctaa tccctatcta ttttctatgg acgtttgtgc tttgttacat ataaattcaa
     6181 gctccctaag gaggttttgt actttgtaga ctgtctcgtg agagtcaaac cttaggatta
     6241 gatagtttta agttatcttt gctaaaatga gttagtctcc ttaaaaataa tcaaaaaagt
     6301 tttttaaaaa cgcttgcaat tatgcttgaa aaggagtata cttataagta acgcaaacgt
     6361 ttgcgtctgc aaaatacgca acgttccatt attttaacac acgaggtgct attatgaaaa
     6421 aacgtcaaag tggtgtgttg atgcacatct cttctcttcc aggagcttac ggaatcggat
     6481 catttggtca aagtgcttac gacttcgttg atttcttggt ccgtacaaaa caacgttact
     6541 ggcaaatcct tccattagga gcaactagtt acggggattc tccttaccaa tctttctcag
     6601 ccttcgcagg aaacactcat tttatcgatt tagatatctt ggtggagcaa ggtttgttgg
     6661 aagcaagtga ccttgaagga gttgactttg gtagcgatgc gtctgaagtt gactatgcta
     6721 aaatctacta tgcacgtcgt cctcttttag aaaaagcggt gaaacgtttc tttgaagtcg
     6781 gagatgttaa agattttgag aaatttgctc aagacaacca atcatggctt gagctctttg
     6841 ctgagtatat ggctatcaaa gagtattttg acaatcttgc ttggactgaa tggccagatg
     6901 cagatgctcg tgctcgtaaa gcttcagcac ttgaaagcta tcgtgagcaa ttggcagaca
     6961 agttggttta ccaccgtgtg actcaatact tcttcttcca acaatggttg aaattgaaag
     7021 cttacgctaa cgacaaccac atcgaaatcg ttggggacat gccaatctac gtagcggaag
     7081 attcaagtga tatgtgggca aatccacatc tcttcaaaac agatgtcaat ggtaaggcta
     7141 cttgtatcgc aggatgccca ccagatgagt tttctgtaac tggtcagctt tggggtaacc
     7201 caatctatga ctgggaagca atggacaaag acggctacaa atggtggatt gaacgcttgc
     7261 gtgaaagctt caaaatctac gatatcgttc gtatcgacca cttccgtggc ttcgaatctt
     7321 actgggaaat ccctgctggt tccgatacag cagcacctgg tgagtgggtg aaaggtccag
     7381 gctacaagct ttttgcagcc gttaaggaag aacttggtga gctaaacatc atcgcagaag
     7441 accttggctt catgacagat gaagtgatcg aattgcgtga acgtactggc ttcccaggaa
     7501 tgaagattct tcaatttgcc ttcaacccag aagacgaaag cattgatagc ccacacttgg
     7561 cacctgctaa ctcagttatg tacacaggaa cacacgataa caatacggtt cttggttggt
     7621 accgtaatga gattgatgat gcgactcgtg agtacatggc tcgttacacg aaccgtaaag
     7681 aatacgaaac agtggtacac gctatgcttc gtacagtatt ttcatcagtt agctttatgg
     7741 caattgcaac tatgcaagat ttactagaat tggatgaggc agctcgtatg aacttcccat
     7801 ctacccttgg tggaaactgg tcttggcgta tgactgaaga tcaattgaca ccagctgtcg
     7861 aggaaggttt gcttgacttg acaacaattt atcgccgaat taatgaaaat ttggtagatt
     7921 taaagaaata agacaataat caggagacaa ctaaacatgt tatcactaca agaatttgta
     7981 caaaatcgtt acaataaaac cattgcagaa tgtagcaatg aagagcttta ccttgctctt
     8041 cttaactaca gcaagcttgc aagcagccaa aaaccagtca acactggtaa gaaaaaagtt
     8101 tactacatct cagctgagtt cttgattggt aaactcttgt caaacaactt gattaacctt
     8161 ggtctttacg acgatgttaa aaaagaactt gcagctgcag gtaaagactt gatcgaagtt
     8221 gaagaagttg aattggaacc atctcttggt aatggtggtt tgggacgttt ggctgcctgc
     8281 tttatcgact caattgctac tcttggtttg aatggtgacg gtgttggtct taactaccac
     8341 tttggtcttt tccaacaagt tcttaaaaac aaccaacaag aaacaattcc aaatgcatgg
     8401 ttgacagagc aaaactggtt ggttcgctca agccgtagct accaagtacc atttgcagac
     8461 tttactttga catcaactct ttacgatatt gatgttactg gttatgaaac agcgactaaa
     8521 aaccgcttgc gtttgtttga cttggattca gttgattctt ctattattaa agatggtatc
     8581 aactttgaca agacagatat cgctcgcaac ttaactctct tcctttaccc agatgatagt
     8641 gaccgtcaag gtgaattgct ccgtatcttc caacaatact tcatggtttc aaacggtgcg
     8701 caattgatca tcgacgaagc aatcgaaaaa ggaagcaact tgcatgacct tgctgactac
     8761 gcagttgtcc aaatcaacga tactcaccca tcaatggtga ttcctgaatt gattcgtctt
     8821 ttgactgcac gtggtatcga tcttgacgaa gcaatctcaa ttgttcgtag catgactgcc
     8881 tacactaacc acacaatcct tgctgaagcg cttgaaaaat ggcctcttga attcttgcaa
     8941 gaagtggttc ctcacttggt accaatcatc gaagaattgg accgtcgtgt gaaggcagag
     9001 tacaaagatc cagctgttca aatcatcgat gagagcggac gtgttcacat ggctcacatg
     9061 gatatccact acggatacag tgttaacggg gttgcagcac tccatactga aatcttgaaa
     9121 aattctgagt tgaaagcctt ctacgacctt tacccagaaa agttcaacaa caaaacaaac
     9181 ggtatcactt tccgtcgttg gcttatgcat gctaacccaa gattgtctca ctacttggat
     9241 gagattcttg gagatggttg gcaccatgaa gcagatgagc ttgaaaaact gttgtcttat
     9301 gaagacaaag cagctgtcaa agaaaaattg gaaagcatca aggctcacaa caaacgtaaa
     9361 ttggctcgtc acttgaaaga acaccaaggt gtggaaatca atccaaattc tatctttgat
     9421 atccaaatca aacgtcttca cgagtacaaa cgccaacaaa tgaacgcttt gtacgtgatc
     9481 cacaaatacc ttgacatcaa agctggtaac atccctgctc gtccaatcac aatcttcttt
     9541 ggtggtaaag cagctccagc ctacacaatc gctcaagaca ttatccattt aatcctttgc
     9601 atgtcagaag ttattgctaa cgatccagca gtagctccac acttgcaagt agttatggtt
     9661 gaaaactaca acgttactgc agcaagtttc cttatcccag catgtgatat ctcagaacaa
     9721 atttcacttg cttctaaaga agcttcaggt actggtaaca tgaaattcat gttgaacgga
     9781 gctttgacac ttggtactat ggacggtgct aacgtggaaa tcgctgagtt ggttggagaa
     9841 gaaaacatct acatcttcgg tgaagattca gaaactgtta tcgaccttta cgcaaaagca
     9901 gcttacaaat caagcgaatt ctacgctcgt gaagctatca aaccattggt tgacttcatc
     9961 gttagtgatg cagttcttgc agctggaaac aaagagcgct tggaacgtct ttacaatgaa
    10021 ttgatcaaca aagactggtt catgactctt cttgacttgg aagactacat caaagtcaaa
    10081 gagcaaatgc ttgctgacta cgaagaccgt gacgcatggt tggataaagt catcgttaac
    10141 atttctaaag caggattctt ctcatctgac cgtacaatcg ctcagtataa tgaagacatc
    10201 tggcacttga actaatactc ttcgaaaatc tcttcaaacc acgtcagctt tatctgcaac
    10261 ctcaaagcgg tgctttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat
    10321 tgagtataag atacaaattt atactaatac attttgtaaa aaagcgagtt tcgattgaaa
    10381 ttcgcttttt taaatgctgt ggatttgggt caatcttgtc taaaaatagg gaaatcctag
    10441 atacagtgaa ggctttaaat gctggttttt actgtcttca gccttatatt ttttcgtagt
    10501 tggttacctc atatctcttg tattcgctta cataaagtat tataatataa ttgtaggaaa
    10561 gaaggtgttt ttatgatata cacacttaaa ttggtgttgt ttattacctt tcttgtaata
    10621 agcttgttac ctgataagat ttttggaaaa aataaaaaaa tttggaaaat agtttttgca
    10681 atattgacgg cagtggcagc attgtcattt atgtactaag ttattttaag aatgtaggga
    10741 aataaaccct acattttttt agttttttct gttttctaaa ttctatttat ccaagagctt
    10801 caacatttct tgcttcttcg cttcaagttc tgcacgcttt tcttcgattt cggcatgttt
    10861 tttctcgagt tcagaacaac ttgcatcatt gctaaattct tttcgccatc aggagatagg
    10921 gtgagtctac atgtctatta ctcacccaaa gcagtcctac aaagcaggaa ttttctgtta
    10981 cttttttgga aatagtaacg tttatacagc tttgacactt cgtatcaaag cgccaaacac
    11041 actccgaggg atttacagaa agcagaaaag gaatgatctg gtataagatc attccttttc
    11101 tctctttttc tttaagtaat tatatacaat gtacgacgaa gtcgtcattg caatgctgat
    11161 ccaccaccta aagggaactt taaacaacat tgataagata aagaatataa acaacgaaaa
    11221 tacgttatac ccaattaatt ttattgtata tctcatgatt aaaagttaat ccttccgttg
    11281 ttaggaatgg catcattttt atcccataat tgtgctaaat aagtccccgg tgataataaa
    11341 ttcatagcga attctaaagc aacatcattt acaaaccaac tacctagata tctagaaatt
    11401 gctgaacgaa tagcactttt tgctgcatgt tttcctttta ctttaattag atttgcaagg
    11461 cctgcagtag ttcctcctaa tgctaaagct attgcagtat ctaatagagc acccatttga
    11521 ttaactgtaa taccttgcca agctgctcta aatggagagt atgtaggtgg gattgtataa
    11581 tcgccttgta attgtcggtt aattacttct ttgatccatt gttgtgagac gtctggatga
    11641 aaagattgga tttcgtttgc aagtgtattg atttgttctt ctgttagaga agtgacaggt
    11701 tgaagttcca tatttgtttc aatttgtgat acttgttcag aagcgtatac agctgaaaca
    11761 cttggaatcg ctgatacaat taacacaatt gacgtcaaaa aaaccgaaat aaatttcatt
    11821 aatttgttca tgagcttttc tcctttttat ttgtatctgc ttacatttta tcatatactg
    11881 ttattatagt caaaaaaata tgctattatg ttaaaaaaat atttttcaaa atataaatgg
    11941 acggatttat tttggatttt atttgttatt ttgacctgcc tctatattgg taaccatgat
    12001 ttgtttactc tcaatcatca agaattctct tttcgtggta gcgtttgggg tctggtactg
    12061 gccttatatc acttattgtt cattgataag tttgtaattt cgaatcgaaa ataaagatta
    12121 gaactatgat tgactggcta cttttaatat tgattttgga ggaagatttt gtctctatta
    12181 tttattattt taaatttatc tattttgtac aagatttatt ctttgagacg ggagaaatcg
    12241 aaatacttga tttatacggc ctatatcata tttggggtaa atgtagtata tggtattcaa
    12301 tggttattaa gagaactgat ttcaactatt tccccttaat gtgtatggaa actataacaa
    12361 aacgcataag attaaggttt gtgtacctgt tcttatgcgt ttttataata ttgaagattt
    12421 tcggtaagtt ttataccttt gggaagtcta gtttagaatg ctttcccaac caaaatctct
    12481 gcttcgatgg taatctctgt cagttggctc cagtcaatct tgctctggtc aacgtgaaag
    12541 aggagaggag tcatactgag tagggcttgg agttgttctg ctgtgatagt cttagtcaga
    12601 gaggcagttt gactagatag gatggtaaag tgttcctgga aatgctcctt gatatcttgg
    12661 ttagaatact ccttgtttgt cagctggtcc tgtactcttt gacggatttc tttgaggtga
    12721 ttttcagttg ggataacctt tatcaagata ccgtctttgg ataaaacgcg acgaaattct
    12781 ccatagttgg caggtgagaa gatatcaagc agaatatcca tgttagcgtc ttttatagga
    12841 agtcgtgcca agtcgccaac aaaccaattg actgcccagt tgggttcact tttagcagcg
    12901 atttggactg aatctttgga gatgtcaaag gcatagaaag ttttttcaga gtgactttct
    12961 tgtagtttgc gagaatagaa tccttcacca caaccgatat ccaaaattgt tgtggtagtt
    13021 tttgagcttg caagcaagtc agatacagca tctaagatag cttggtaaaa gccggcttct
    13081 aggatttgtt gacggttttg aaaattttcc ttgttatagt tagcagattg cttgatttga
    13141 ggtgctagat tgacatagcc aaatttcgcc aagtcaaaag aatgacggtt gcagcacttg
    13201 aaattagtct ctaacagagt cagattttct tgacagatag gacaggcaaa ggcagtcgca
    13261 gaagcaaaac gctgaagttt gggcttgaga tttgtattca tagtttaagt gtatcaaaag
    13321 aggcaaatga aagcaacttt aggaataagt agatgggaga ttcagtagaa ataaaactaa
    13381 ttctccaatt tatagaatga aattgctttt atatctaaat tgctatataa taatgataag
    13441 gaggaaataa gtatgttaaa agaagtatta accgttgcaa aagttgcgaa aaaatcttca
    13501 ttatttttgg gtggtgtcgc atttggtacc cttggtttga aaatcttagc aagtaaggaa
    13561 gctaaaaaag gttattctaa agctttggct aaggcttaca agttgaaaga cgagctagat
    13621 gcatctgttt ctgttgtgaa gcaacatgga gacgatgtct tgcaagatgc caaatatttg
    13681 tacgagcaag agaaaaaaga agagcaatta gatagcctta taggtgaata atatgtcttt
    13741 taaagtgcta catagaggat accaacatat ccgactatca tcttcttttt cacttacctt
    13801 ggatattcaa gactatcttc gttccttggc gagagatgaa aaggggattg agtctatcca
    13861 gttttacatg gatcaacagc actttactct acgcataaaa gaaggctttt ctgtattaga
    13921 taatgcagaa gcctttttaa aaagaattga taaagggaaa gtttctgagt tgatgactct
    13981 tcccattcgt agagaagaga gtgcttattc tattgtttca ggtgcagcgg ttaagcgtgt
    14041 actttttcgt agttttgtgc cgtatcctat tcgctatata tggacttgtt atcaggcttt
    14101 gggttatatt agagaagcct atcaaacact agcgcgtaag gaactaacga tggaagtctt
    14161 ggactgttcg gctattttat tgtctttgtt tatgaaccaa tccaagacag ctagcaatat
    14221 catgtttatg cttgatttgg ggaatcattt agatcagtgg tccttgaaaa aaactgcaac
    14281 agatttagaa cagagtcttc ttgcaaaaga gagcgatgta ttcctagtac agggcgatac
    14341 ggttgttagt atcaagagtt ccgatgttca aataggagat gtcttgatct tatctcaagg
    14401 aaatgaaatt ctgtttgatg gacaagtagt ttcaggttta ggtatggtca acgaaagttc
    14461 cttgacagga gagagttttc cagttgaaaa aagagagtct gatttggttt gtgcaaatac
    14521 agtattagaa actggagagt tacgcattcg tgtaacagat aatcagatga acagccgtat
    14581 tttacagctg attgagttga tgaagaaatc tgaagaaaac aagaaaacga aacaacgcta
    14641 tttcatcaag atggcggata aagtcgtcaa atataatttc ttgggggctg ggctgactta
    14701 cctattgaca ggttcttttt ctaaggctat ttctttccta ttggtcgatt tctcctgcgc
    14761 tttgaaaatc tctactcctg tagcttattt gacagttatc aaggaagggt tgaaccgtga
    14821 aatggtgatt aaggatggag atgttctgga gaaatatctg gaagttgata ctttcttgtt
    14881 tgataagaca ggaacaatca caactagtta tcctatagtt gaaaaggtgt taccttttgg
    14941 agactatagt gaggaagata ttctcagaat cagtgcctgt cttgaggaac acatttatca
    15001 tcctattgct aatgccatcg tcaagcaagc tgagatagag ggaattgaac atgaggaaat
    15061 gcatgggaaa ctccaatata tcgcaagcaa ggggatcaaa tctcatatag atggccaacc
    15121 agttcttatt gggaattatg tcttgatgca ggatgagcag attcatatca gttcagaaca
    15181 aaatgcttta attgaagagt acaagagtca ctacaatctc ttattcttgg cttatcaaaa
    15241 tgaattgatt ggaatgttct gcattcatac tcctttgaga aaagaagcaa aaacagcctt
    15301 ggataaactt aaggcacagg ggaaaaaatt gattctggca acaggggaca ccttggttag
    15361 gacaaaggaa ttagtcaaag atttgccctt tgatcaggtc tatacagact tgaaacctga
    15421 tgggaaattt gagttagtag agaaactgca gaaagcaggt cacactattt tgatggttgg
    15481 agatggattg aatgactcag cggctttaac tctatcagat atcggtgtgg tgatgaatga
    15541 gagtgcagat atttctaagc agatgagtga tatcttattg ttagataatc gtttggattt
    15601 cttccaagag ttggattcgt tatcatcatc tttgcaaaca ctcatcaaga aaaatattca
    15661 agataccgtt gtcgtaaata gtagtttgat tggcttcggc ttatttaatt ggctcagtcc
    15721 ttcaaatctc tctatcttac ataatctaac aaccttgcgc attgtcctgc gtagcctgtc
    15781 tattaaaaat agatagatga gagttattaa cagcaaaaag gagttacctt tctcgaggtt
    15841 aactcctttg tttatagata aatgttgaat agtttagtaa gtcaatacaa agtatttttt
    15901 cttcccacga cggataacag tcagttcatt ctctaactta tcagcgtcac tcaagacata
    15961 gtcaagctct tggatgcggt cgccgtttac gtagatggct ccgttttgga cgtcttcacg
    16021 ggcttggcgt tttgagttaa ctataccaga tgagacgagc agttccacga tattgttgtt
    16081 ttcgtctgcc tgtacttggt agtttggcac accacgaagt ccttgtttga gctctttgac
    16141 agaaaggttt ttgatgtttc ctgcaaagag ttgctcagtg atgttaagtg cttctttgta
    16201 ggcttcttct ccgtgaacaa gtgtaacaac ttcacgagcc aagacttttt gagccaagcg
    16261 ttcgtgtggc gctgcttcaa attgtttacg aatatcttca atctcatcaa gtgacaagaa
    16321 agtaaagatt ttcaagaagc gaacagcgtc agcgtccatc acgttcatcc agaattggta
    16381 catttcgtat ggagaagtct tttcgggatt gagccagacg gcatttcctt ctgatttacc
    16441 aaatttctta ccagttgcat ctgtgattag tggaacagtg ataacgtgac cagtcttgtc
    16501 cgccttacga cgaagcaatt cggtaccagc tgtcatattt ccccactggt cagaaccacc
    16561 gatttgaaga gtgacattat ggtcttggtt aaggacgaag aagtcatacc cttgcatgat
    16621 ttggtaagcg aactcagtgt aagaaattcc tgtttcgatc cgttttttaa cagattcctt
    16681 actcatcatg tagttgaccg tgaagtattt tccaatatca cggaggaagt caatgaagct
    16741 gatgctgcca aaccagtcgt agttgttgac catgacagcc ttgttttcgc cattttcaaa
    16801 gtcaagaaaa cgagaaagtt gtccttggat agacttgacc cagccatcta ctgtgtcttt
    16861 tgtttggaga ctacgttcag catctttgaa ggacggatct ccgatgagac ctgtagcacc
    16921 gccaacgagc gcataaggtt tgtgacctgc tagttgcaag cgacgacttg tcaagattgc
    16981 gacaaggtgg cctaggtgaa ggctgtcagc agttggatcg tagccagtat aataagaaac
    17041 ttgaccttct tctagggctt tacgcaaagc ttcttcatca gtcgtttgaa atatcaaacc
    17101 acgctctttt agctcatcaa aaatgtgcat gtgttttttc tcctttttaa aattattgtt
    17161 tctacctatt gtatcacaaa cttgggcaat agcctagcag aatagggaag aaagtggcta
    17221 ttttattgaa aatttccctt ttgtggtata atagataaag tgaggaaatc catgcaaaat
    17281 caattaaatg aattaaaacg aaaaatgctg gaatttttcc agcaaaaaca aaaaaataaa
    17341 aaatcagcta gacctggcaa gaaaggttca agtaccaaaa aatctaaaac cttagataag
    17401 tcagccattt tcccagctat tttactgagt ataaaagcct tatttaactt actctttgta
    17461 ctcggttttc taggaggaat gttgggagct gggattgctt tggggtacgg agtggcctta
    17521 tttgacaagg ttcgggtgcc tcagacagaa gaattggtga atcaggtcaa ggacatctct
    17581 tctatttcag agattaccta ttcggacggg acggtgattg cttccataga gagtgatttg
    17641 ttgcgcactt ctatctcatc tgagcaaatt tcggaaaatc tgaagaaggc tatcattgcg
    17701 acagaagatg aacactttaa agaacataag ggtgtagtac ccaaggcggt gattcgtgcg
    17761 accttgggga aatttgtagg tttgggttcc tctagtgggg gttcaacctt gacccagcaa
    17821 ctaattaaac agcaggtggt tggggatgcg ccgaccttgg ctcgtaaggc ggcagagatt
    17881 gtggatgctc ttgccttgga acgcgccatg aataaagatg agattttaac gacctatctc
    17941 aatgtggctc cctttggccg aaataataag ggacagaata ttgcaggggc tcggcaagca
    18001 gctgagggaa ttttcggtgt agatgccagt cagttgactg ttcctcaagc agcattttta
    18061 gcaggacttc cacagagtcc cattacttac tctccttatg aaaatactgg ggagttgaag
    18121 agtgatgaag acctagaaat tggcttaaga cgggctaagg cagttcttta cagtatgtat
    18181 cgtacaggtg cattaagcaa agacgagtat tctcagtaca aggattatga ccttaaacag
    18241 gactttttac catcgggcac ggttacagga atttcacgag actatttata ctttacaact
    18301 ttggcagaag ctcaagaacg tatgtatgac tatctagctc agagagacaa tgtctccgct
    18361 aaggagttga aaaatgaggc aactcagaag ttttatcgag atttggcagc caaggaaatt
    18421 gaaaatggtg gttataagat tactactacc atagatcaga aaattcattc tgccatgcaa
    18481 agtgcggttg ctgattatgg ctatctttta gacgatggaa caggtcgtgt agaagtaggg
    18541 aatgtcttga tggacaacca aacaggtgct attctaggct ttgtaggtgg tcgtaattat
    18601 caagaaaatc aaaataatca tgcctttgat accaaacgtt cgccagcttc tactaccaag
    18661 cccttgctgg cctacggtat tgctattgac cagggcttga tgggaagtga aacgattcta
    18721 tctaactatc caacaaactt tgctaatggc aatccgatta tgtatgctaa tagcaaggga
    18781 acaggaatga tgaccttggg agaagctctg aactattcat ggaatatccc tgcttactgg
    18841 acctatcgta tgctccgtga aaagggtgtt gatgtcaagg gttatatgga aaagatgggt
    18901 tacgagattc ctgagtacgg tattgagagc ttgccaatgg gtggtggtat tgaagtcaca
    18961 gttgcccagc ataccaatgg ctatcagacc ttagctaata atggagttta tcatcagaag
    19021 catgtgattt caaagattga agcagcagat ggtagagtgg tgtatgagta tcaggataaa
    19081 ccggttcaag tctattcaaa agctactgcg acgattatgc agggattgct acgagaagtt
    19141 ctatcctctc gtgtgacaac aaccttcaag tctaacctga cttctttaaa tcctactctg
    19201 gctaatgcag attggattgg gaagactggt acaaccaacc aagacgaaaa tatgtggctc
    19261 atgctttcga cacctagatt aaccctaggt ggctggattg ggcatgatga taatcattca
    19321 ttgtcacgta gagcaggtta ttctaataac tctaattaca tggctcatct ggtaaatgcg
    19381 attcagcaag cttccccaag catttggggg aacgagcgct ttgctttaga tcctagtgta
    19441 gtgaaatcgg aagtcttgaa atcaacaggt caaaaaccag ggaaggtttc tgttgaagga
    19501 aaagaggtag aagtcacagg ttcgactgtt accagctatt gggctaataa gtcaggagcg
    19561 ccagcgacaa gttatcgctt tgctattggc ggaagtgatg cggattatca gaatgcttgg
    19621 tctagtattg tggggagtct accaactcca tccagctcca gcagttcaag tagtagttct
    19681 agcgatagca gtaactcaag tactacacga ccttcttctt caagggcgag acgataattc
    19741 tctaaatgaa gtggccaatc aatggattgc cacttttttc ttttcgtgtt acaataaggg
    19801 tatgaatcag tatcagaaaa agattgttaa cggaaaaatt tattcgctcc tatccggctt
    19861 aatatgggga atctgtggaa ttttaggaga gtacttcttt actcattatc aggtgtcttc
    19921 gggctggatt acctctatgc gtttgacact ggcagggagt cttgtactca tttggtctgc
    19981 aatacaatta aaatcgcaag tgctagatat ttggcgagac aagaaaaatt acctgccctt
    20041 tttagcctat gctattttgg ggattttttc agttcagtat tttttctatc tctgtgtaga
    20101 atactcaaat gctacgacag caactatttt acagtttatt agccctgtct ttatcctctt
    20161 ttacaatcgc ttggtttatc aaaaacgagc gtcaaaaagc gctgttttct atgttttggt
    20221 tgccatgctg ggtgtttgct tgatggcgac aaagggagac ctctctcagt tatccatgac
    20281 gccgctagct cttataacag gtttgctgag tgccatgggt gttatgttta atgttatctt
    20341 gccccaacct tttgctaagc gttatggttt tgttcctacg gttgggtggg ggatgatttt
    20401 ggcaggtttg tttagcaatg tcctctcgcc ggtttatcag ctttccttta ctcttgatat
    20461 ttggagtatc ttgatttgcc tcattatcgc tttctttgga acggcttttg cttttttcat
    20521 ttccatgaag gctgtgtcct tggtttctcc tttggtggtt gccgttatca gtgccagtga
    20581 acctctctct tctgctctct tgagtgtttt gttcttagga ttagtagtgg attggtccct
    20641 ccttctagct atagccttga ttattttacc catgatattt ttgtctatag aagaagcgaa
    20701 agaaagtaga taaaaagtct tttcttaatt ctaaaagtgt gctatactag aatagtcaat
    20761 aaaacacggg gagatagttg tgaagagtgt ttttaggttg tatcggtagg ggcttgcttt
    20821 taccgacagc acgttttatc tcacatccct aaaaaatcat acctaaaaac agacaaaaag
    20881 gtttcaaatt tgaggccttt tttggtagaa taggtatcat tataacgaac caggaggcac
    20941 ctatgactgc tacaaaaatg aacgctcaag aaattatcca atttatcgcc aatgctgaaa
    21001 agaaaaccag tgtcaaagta acctttgagg ggcaactcgc aactgctgtg cctagctctg
    21061 ttgtcaaact aggaaatgtc ctattcggag actggaagga cgtggctccg cttcttgaag
    21121 gtttggtaga aaatcaagat tatgttgtcg agcaagatgc tcgtaattct gcagttcctt
    21181 tgctagataa gcgtgctatc aacgctcgta tcgagccagg tgctattatc cgtgaccagg
    21241 tggaaattgg tgacaatgct gttatcatga tgggagctgt tatcaatatc ggtgctgaaa
    21301 tcggtgctgg aaccatgatt gacatgggtg ccatccttgg tggccgtgcc atcgttggaa
    21361 aaaatagcca cgttggtgca ggtgcagttt tggcaggtgt gattgagcca gctagtgctg
    21421 aaccagtccg tgtcggagac aatgttctca tcggggctaa tgcagtggtt atcgaaggag
    21481 tccaaatcgg tagtggttca gttgtcgcag caggagctat tgttacccaa gatgtcccag
    21541 aaaacgtggt agtagcaggt gttccagctc gtattatcaa agaaattgat gcccaaactc
    21601 aacaaaaaac agcgctagag gatgcgcttc gtaccttgta attgtaaaag taaaaaagag
    21661 gcggaaccct ttttccagcc tctttctgct atatcggagg acagatagat gttagatttg
    21721 attcagacta gacgagattt acaccagatt ccagagattg gcttggagga gttcaagact
    21781 caggcttatt tgctggatgt gattgagaaa ttgactacgg gcaaggattt tgttcaaatt
    21841 cgtacttggc ggacagggat tttggtctac ctgcagggaa gtcagccgga gcgaaccatt
    21901 ggttggcgga cagatattga tggcctgcct atcgtcgaac aaacaggtct accttttgct
    21961 tctcaacacc aaggtcgcat gcatgcctgt ggccatgatt ttcatatgac cattgcctta
    22021 ggctgtcttg agcgcgccct tgaggagcaa cccaagaata atctgctctt tctatttcag
    22081 cctgctgaag aaaatgaagc tggtggtatg ctcatgtatg aggacgatgc ttttggagat
    22141 tggttgccag atcaatttta tggcctccat gtccgtccag atttgaaagt cggccagatt
    22201 gcgactaata ctcatacact ctttgcagga acttgcgagg tgaagatccg tttcaaaggc
    22261 aaaggtggcc acgcagcttt tcctcatgaa gccaatgacg ccttggtggc ggctagttac
    22321 tttgtgaccc aagtgcagtc agttgttagc cgcaatgtca acccaatcga gggagcagtg
    22381 gtgaccttcg gcgtttttca agctggaacc accaacaatg tcatcacaga cacagctttt
    22441 ttacatggaa ctattcgtgc cttgacacag gacatgagtc tcttggtgca aaagagggta
    22501 aaaacagttg cagaaggggt tgcagctgcc tttgatatgg aagtcgaagt ggaactcaaa
    22561 caaggtggat acctacctgt tgagaacaat ccagccttgg cgcgtgaact gatggacttc
    22621 tttgacgaaa aagacggaat cgagttgatt gatatcgaac ctgctatgac aggcgaggac
    22681 tttggttatc tcctttcaaa ggttgatggc gtgatgtttt ggctaggtat cgatagtccc
    22741 tacgctcttc atcaccctca aatgagtcct aaggaagaag tcttagctat tggggtggct
    22801 gcggtgtcta gtttcctgaa aaagaaggca gcagaataga gggcttgtct atgaaatcgg
    22861 aattacgcaa gcaagtcttg catgaaatga aggctatatc tcaagagcaa aaacaggcta
    22921 tagaccaagc tttaaccgag cgaattttac aacacccctt ttatcaagaa gccaaggtca
    22981 tcgcaaccta cctctctttt tctcatgagt ttcaaacgcg ggaactgatt gagcaggcgc
    23041 tgaaggacgg caagaaggtt ttaataccca aaacttatcc caaggggcgc atggactttg
    23101 tagtctatga tccgcagcag ttggtaaaaa ctgcctttgc cttactggag ccacagggag
    23161 atttggaagt ggtggatgca tctcagattg atttgattca tgttcctggc ctggctttta
    23221 cgacggatgg atatcggatt ggatatggtg gaggttatta tgaccgctat ctggaacatt
    23281 tttctggtca tactttgagt acggttcatc cttgtcaaat tcaggacttt atacctgaaa
    23341 accatgatat tcctgttcag gaggtattaa ttgatgaagg aaatctttga tagacgttac
    23401 cctgtgacga gtttcttcct cttagtgacg gccttggtat ttttactaat gttggtcact
    23461 gcaggcggaa actttgacag ggcagataca ttatttcgat ttggagccat gtatgggcca
    23521 gctattcgcc tctttcccga gcaggtttgg cgtctcttgt ctgccatttt tgttcatatt
    23581 gggtgggaac atttcattgt taatatgctt tcactttatt atcttggaag gcaggtagag
    23641 gagattttcg gttctaagca gtttttcttt ctctatcttt tatcaggaat gatgggcaat
    23701 ctctttgttt ttgtatttag tcctaaatcc ttagcagcag gcgcctctac ctctctttat
    23761 gggctatttg ccgcgattat tgttcttcgc tatgcaactc gcaatcctta tatccaacag
    23821 ctagggcaat cctatctgac actttttgtg gttaacatta ttggaagtgt tctgattcca
    23881 ggaatcagcc tagcaggcca tatcggtggt gcagttggtg gcgcatttct agcagttatc
    23941 tttccagtta gaggagaaaa acggatgtat aacaccagcc agagattagg agcggtagtc
    24001 ttgttcgtag gactcgccat tttgcttttc tacaagggaa tgggaatgtg acgaagttga
    24061 aagaaaattt gtgtaatgaa aaaagataag gcattttttg aaccttatct ttttatttta
    24121 ttttattcct tctcagtcaa ttcttttcca agttggatga ggtaattctt caaatcatct
    24181 ttgacttgtg ggtgtttgag ggcgtagtcg atggatgttt tcatgaagcc aaacttgtct
    24241 ccgacatcgt aacgagcccc attgaactca cgagcaaata cacgttgtgt tttattgagg
    24301 gtgtcgattg catctgtcag ctgaatttca tttcctgcac ctggagcttg gttttcgagg
    24361 atttggaaaa tttcaggcgt gaggaggtag cgtccgataa tagcaaggtc gctaggagcg
    24421 tcctctggag ctggtttttc aacaaaggtt tcaacactgt aaagaccatc ttttccttcg
    24481 ccttgcggag caataacccc gtaagcagat acttcgtcat gagggactgg catgacagcg
    24541 atagtagacg cgtgggtacg ctcgtagtca tccatgagtt gtttggtaag tggaacagcc
    24601 ttttcgtctg tgatatccat caagtcatca ccaagcataa cgacaaaagg ttcatttccg
    24661 acgaaagcct tggcttgcaa aacagcatct ccgagaccgc gtggatgagt ttggcggata
    24721 aaatgcagcc gcatgtcagt tgttttatca actagcttca aaagatctgt tttccctttt
    24781 tctttgaggt tatattccaa ttcgaagttt gaatcgaagt ggtcctcaat agaacgtttt
    24841 gatttaccag tgacaactag aatatcttca atacctgatt tgagagcctc ttccacgata
    24901 aactggatag ttggtttgtc tacgattggc aacatttctt tggcaagggc cttggttgct
    24961 ggtaaaaatc gagttcctag tccagcagca gggataactg cctttctaac ttttgatgtc
    25021 ataaaaatcc tttctataga gggttaagac cactcatttt ctgctttaaa ttcattgttc
    25081 atgatgtcat aaatggcatc tttgatattg gttccgtgat aaataacttg gtaaatagcc
    25141 tgtgtaatgg gcatatagac tccaagttct tgggctagtt cataggctgc tcgagtcgtt
    25201 gaaattcctt cgattaccat gcccatatta gcttctatat cagctaggga ttctcctcgt
    25261 ccgagagcat ctccagctct ccagttacga gagtggatgg aagttcccgt tacgatcaaa
    25321 tctcccacac cagataagcc gctataggtc aatggactgg ccccgagtgc tacccctagg
    25381 cgggtgattt ctgctaaacc tcgagcgatg atggctgcct tagcattatc accaaatcca
    25441 agaccatgta aagctccagc accgacagca ataatatttt taagagcacc agcagtttca
    25501 accccgataa catccgtatt ggtataaagt cggaagtagt gattactaaa tagctcctga
    25561 acgtattgag ctgtttgtaa atctttagaa gcagcagtta ttaaagttag gtcacgcaca
    25621 atggtctctt ctgcatgact aggccctgaa acaacgacga tatcactacg gagatgttca
    25681 ggaatttctt cttcaagaat ggttgataat cgtttatggc tatcaggttc taatcccttt
    25741 gatgcgtgca tgatgataac cttatggtcc aaggtttgtg caacttgctg ggcaacaagt
    25801 cgtgtcactt ttgttgggac aacaaacaaa atcgcatcca catttttcaa tgtttctgct
    25861 aagtcggtgt aggcaatgat attttcgtct agaacgacat ctttaaagta gtgcttatta
    25921 gtatggtgtg tattaatttc attgatttgc tcgggaagat ttccccaaat acgtacctcg
    25981 tgtccattgt catttaagac ttgtgaaagg gcggttcccc aagaaccagg ccccaagacg
    26041 gcgacggttt gtttttccat gatttcctcc ttgatagatt tctttctctt attctatcat
    26101 attctagggg attttgcact tgcattactg gggtatggtg gaaaaataca caaagaccga
    26161 gatgaaggga taaactctta gtctttagtt aaagtgagaa gaaatctgat agatagtttg
    26221 attcagtata tgcttataat agaggtaaac aactcaggaa gttcttgtag gttgcgagag
    26281 tttggcgaaa aaattaaaag attacgtttg gctaaaaaaa tcagtcgttc agaattttgt
    26341 ggtgatgagt ctgaattaag tatccgtcaa ttaattagaa ttgaaaatgg agaatccaga
    26401 ccaacactaa caaagttaaa atatattgct gaacgtttgg aggttgaaga ttacaagttg
    26461 atgccaagtt atatagagtt ggataaggaa tacctagaat tgaagtattt cttgatgagg
    26521 actcctacat acgaagatga aactatcgcc caaaagaaag agagtgtttt tgataagatt
    26581 tttgaagagt attatgatag gctacctgag gaagaaagat ttatcatccc aaattattca
    26641 tatctagcac tagcgaacta cacagttcaa aaattaccag aaaagctagt tgaaatactg
    26701 tccttctggt gattttgctt aatttctgat gttcgagaca tgattgagga gatttgtctt
    26761 gattttttcc taaaaaaggg cagactcctc ccttggtttg atgctcgatt tttaaaataa
    26821 ggtgacttat tctgttaggc agttttcccc tgccctctag ctctatatgg aacagggagt
    26881 atcagattta tctggcagat tgtatcaaat aaggcttgaa actgttttga ataggcgtat
    26941 agacttgaga atttgagaat atgtcgtcta gcagtagaga catattttcc ggcaatatga
    27001 aggaagagac gtcgaaaacg cttgatagtc aaggccttta cttcatcacc agctagctgc
    27061 tttaaaaaga ggtagagatt ataagccaga cagcccatca tcatacgtac ttcattctta
    27121 atcatggtcg aactatctgt cttatcccca aagaatcctg ctttcctttc tttgataaag
    27181 ttttcagcat tccctcgttc acgatagaat tgaaacagct cctcactatc tacactgctt
    27241 aaattggtca aatgaacttt tttacaacca taaaaaagag cacttaatcg tactcttctc
    27301 ttttaaggat tagttgaatt aattcaaatc cactagttct cctgtttcta ggtagactag
    27361 gcgttcacaa atgttagcaa tgtaatcagc gaagcgttcc agatgcccta tgatataaag
    27421 gtattgagtt ccattgggaa ttgaggtttc ttggtctttc ataagtccaa tgatttcctt
    27481 tgataaggca taataatatt ggtcaatctg ttcatctttt tgagcaatac taatagcttt
    27541 tgaggcttgg tgcaaaggaa aggcaaccaa taaatcggct agcatgctga gggataattt
    27601 acccatttgg tgtaactgtt cttcgtcagg ggctagttga ttttctttta gttgcaaaac
    27661 agctttggca atgcctgcca tatggtctcc catacgttca aggtctgaac aagaagacat
    27721 gatgctaatc acaaatcgaa ggtcagacac ttgtggctgc tgcaaggcca acaaacgggc
    27781 acaggtcaat tcgatagcgc tttgaccttg gtttatagca tgatccttat tgataattag
    27841 ctctgccatc tccttgtctt tggaggctaa ggccagtaag gcttttgaag ctgtttcaag
    27901 gacaagttgc cctagtccta aaaaggattg ttctaattca tgcaattcta agtcaaattg
    27961 atttctcata ggtttttcct tccttatccg aaccgtcctg aaatatagtc ttctgtgcgc
    28021 tgatcttttg gattggtaaa cacgtcaacg gtatctccaa attcgcaaat ttctcctgtt
    28081 aagaaaaaag cagttttatc tgaaatacgt gaagcttgtt gcatgttatg ggtaacaatg
    28141 ataatcgtat aatccttttt tagttgctga atgaggtctt caatttttaa agtggagata
    28201 gggtctaagg ctgaagtcgg ctcatccatt aacagaatat caggttctac tgctaaagct
    28261 cgcgcaatgc aaaggcgttg ctgctgaccg ccagataagg acatggcact ctttttaaga
    28321 tcatctttga cttcttccca aatggctgcc ccttttaaag atttctccac taaggcatct
    28381 aattgttttt tgtctcgaat accatgtgtc cttgggccat aagccacgtt atcatagata
    28441 gacatggcaa agggattagg ctgttgaaaa accatcccta cacgcttacg tagctgatta
    28501 aggttgaatt tgctactata aatatcttgc tcatctaaga ggacttggcc ttcaatatgg
    28561 caagaaggaa ccaaatcgtt catccggtta agggttttta gaaaagttga tttgccacaa
    28621 ccagatgggc ctatcaaggc agtaatctgt ctttctggta attgaatcga aatatttttt
    28681 aaggcttgaa aatccccgta aaataagtct aggtgtctga ctgaaaatgt tcccatacta
    28741 ggaagctcct ttcacaagtt ttcgagataa taagcttgat agagtattta tcattaaaac
    28801 agtaataatc aaaatcacac cggtagcata ggcttcattg acatgtagcc cctcacttga
    28861 cagcatatac atatgcaggg ctagagaacg acctgaagac atgagactac ttggcgtatt
    28921 ggtagaggta cctaatgtat acatgagggc agctgtctca ccaacgatac ggccaatagc
    28981 tagtatcact ccagctaaaa tacctggcat ggcaactggt agaacaattc taaaaacagt
    29041 ccgtaactta cctgccccaa gtccataact tgcttgacgc atgctatcac taacagataa
    29101 aagggcttct tctgttgagc gaataatgac tggcaacacc atgatgactg aggttaagat
    29161 tcctgataac agagagtatt gaaaacctaa gaagactaca aagaagagca tgccaaacag
    29221 accaaaaaca atggaaggaa tcccagacaa ggtatctgag gccaatcgca tgattttaac
    29281 acaaagggaa tctttttttg tatattccac aagataaaaa ccagcaaaaa tccctatggg
    29341 caaggctaaa agaagagcac caaagaccag aataacggtg gaaataatcg ctggcataag
    29401 ggaaatgttc tcagaagtat aagtccaaga aaagagggat agacttagat gaggtaaacc
    29461 tttgatgagg ataaaaccaa tgattaaaaa gagagagcca aaggttaaag ctgaaaaaca
    29521 ataaacgaga agttttagca ggtatttact cataagatga ttttcctttc aagtaggcaa
    29581 agtaggcatt aatcaagaga ataaggaaaa agagaactgc tgaggttgca ataagggctt
    29641 ccctatgctg acctgatgcg taagccattt ccagaacaat attggttgtt aaggttctgg
    29701 ttcctgaaaa gagtccactt ggaataatcg gctggttgcc tgccaccaaa ataactgcca
    29761 tggtttcacc tactgcgcga ccgatgccta aaataactgc tgataaaata ccagatctcg
    29821 cagctggcaa gatgacacta aaaatactcc gttcatgact agctcctaga gccaagctac
    29881 cagaataata cgttttggga actgttcgga tagcagattc tgacaaactg ataatggttg
    29941 gcaaaatcat tattcctaat agtaacgaag cggttaggac actcatgcca tttcctaaaa
    30001 agcttctaat ccaaggcacc aataattgta ggccgaaaaa accataaaca atagatggaa
    30061 tggccgccat caagttgata gctgatttta agaagccata gacgggcttt ggacaataat
    30121 aaaccataaa caccgatgtc aagatgcctg ttggcacccc aatcacaatc gctcctaagg
    30181 taattaataa ggaaccaacg atcattggta aaataccata gcttgccgga atgttcgttg
    30241 gcgaccaatc actgcctaat aaaaaacggg caaagccgta gttagccatg aaaggtaagc
    30301 cattactaaa aataaagaaa cagattagca aaatagctac aacagctact gttgcactca
    30361 tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg ttttatcaca tcttgtcctt
    30421 tctagtgaag aaagtaaggg agatacgaca cctccctact tgccttcttt atcttattgt
    30481 acgatgaaac gtctgcatct ctttagagat ttatggagca aacattttat ttaatcttgt
    30541 cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc agccatactg acttggcttg
    30601 ccttattgtc attattgacc acaacagcaa taccgtctaa agcaatagca tcatgggtga
    30661 gactcttacc ttcttcagga gttaattccc tagaaaccat accaatatca gcggttttct
    30721 ccttaacagc ggtaatacct gctgaagacc cattagaggt aatatcaatc gtaacttctg
    30781 gattttcttt tttataagct tctgctaatt tttccattaa agaagatact gaagtggaac
    30841 ctacaacaga caacttgcct gataagtgtt ggcttgtata ttctgtggtt tcagttttag
    30901 cttcaataaa tttattatct gtgaccactt gttgaccttg tttggagtgg ataaagctga
    30961 taaaatcttg acctagcttg gaaagattag aagaccaaac aatgttgaag ggacgttgaa
    31021 gagggtattc accatctaaa actgtgtctc gactagcctt gacaccatca atctctaaag
    31081 ccttgacaga tttcgttaaa gatcccaagg agatgtagcc gatagcatta gcattccctt
    31141 gaactgctga gagaacacct tctgtactat tttgaatcac agctgttttg gcagtgttgt
    31201 caattttttt atcaccgtct tttttgagaa tccctgtgat ttctgtgaag gcaccccgtg
    31261 ttccagagcc attttctcgt gaaatcacct caatcgttcc tgaagctgac tgtttggaag
    31321 cagctgactg attgccacag gcaacaagcc caaatcctga taagccaatg gctgcaagag
    31381 taagcatttt tttgaatttc ataataatca cctttatctc tatgtatttt tcttgtgtag
    31441 gcttactaca tttatagtct aacaagtctt tgtaaaggtt tatccctgat tcatgtaaag
    31501 attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg ctgctcctaa aaatagcttt
    31561 aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa aaatcgtgaa gcaactgcct
    31621 ctgccaagct gactcgtcac cgtgacttgg ccacctaata attgactgag ttctttgaca
    31681 atggcaaggc caagaccagt gccaccagtt tgtctgcttc gacctttatt aactcggtaa
    31741 aaacgttcaa aaatacgatc ctgctctaat tgactaatac caatccctgt atctgataca
    31801 gaaatcttaa tgccttcgtt caccttttgg gtcttgacct caatttttcc cccttgttca
    31861 gtgtaacgga tggcattgga taaaagattg agtaagattt gggaaagtaa ttgactatct
    31921 gatacgaggg tgacatcatc tggcacctgc acctttagct gtaaatcctt cttcttgagc
    31981 tgaggttgca agctttgagt caaatcctgt acaaattcag ccaaagaaag ggtcgtccat
    32041 tgtataggca tttgttgagc cttagataag gtaagaagat gctcaacaat atgctcaaga
    32101 cgcaaacttt ctttgtaaat aatgtctaga aagtcatcct tgagcgcttc ttcttcagct
    32161 gacatcccct taatggtttc agcaaagccc ttaattgaag taactggtgt cctcaattca
    32221 tgggaggcat ttgagacaaa ggctaaattt aacttttcat aagttctaat cgttgttaaa
    32281 tcatatagca agacgagcac agcttccaca gattgggtgg ggctaaaaac gggaactgct
    32341 gtcacttcta aaatcaagtc accctcatga aacccactta cttcttgttt taaccttgtt
    32401 ttttgatcaa aggcttggtg aactaaattc cgaatatcca tccgtttgag gtcatcaagt
    32461 gaacttatgt cgccgtccac atcgggaaaa taatgaggca gagagcgact ggataataac
    32521 atctgacctt gagcggaaac taaaaacgtc cccatggtta ggtgcgacag aagaacctcc
    32581 attgtttcgg ctaggtcctt gtattgctga tcctgttggg agactttggt ttttaggcca
    32641 gacacatact gagccaaaga ctttaagtct tcttgccctt tttctaaaaa gtattcacta
    32701 ctggtcaaga gaggttggtg caaggtctca aaagcaactt cccatttcca aaggcaaaag
    32761 agccagtagc caccttgtcc caaagaaagg gctagaagaa agagaccgat gcctttactg
    32821 atccaagtta atgccatccc tgcaatcaga atgaggctaa cacttagatt gactagccaa
    32881 aattgaaggt agcgtttcat ctataactcc ttgaacttat aaccataacc ccgaatggtt
    32941 cgaataaatt gaggggcttt aggattgtct tcaatttttt ccctcaactt accaatatga
    33001 acgtccacca aacgtgtttc ctgcccaaag tcataccccc agatacgttc caaaagacgc
    33061 tctctagtca gtgtcatgtt gggatgtttc ataagataga gcaagagttc aaattctttt
    33121 ggggtcaaac tcagtaactt attcgccttg tagacttcat gacgctcagg gtatactttc
    33181 aaggtcccaa atagccaaga atcgtcagcg atattatctg aatcatctcc ttcttgttct
    33241 cctttagttc gcctgaggac agccttgaca cgcgccagca attctctagg gctaaaaggc
    33301 ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa ccttatcaaa ttcatcactt
    33361 ttcgcagaaa ccatcataat tggagttttg acgcctttgg ctctcagccg cttacaaact
    33421 tccatgccat ctaattgtgg taacatgata tcaagcaaga taaaatcaaa gggttctgtt
    33481 tctgccaaag ctaaggcctt ccgtccattt gttaccaatt gagtagaaaa gccttcctta
    33541 cttaaatggt agtcaagcaa tttcagaatg tgttcttcat catccactaa taagacttgt
    33601 tttgtcatct attatctcct attggtaaca ttataacaca attatcagaa atcctaacat
    33661 tgctaaatca gattaaattt gcttatcaag actagtatct ggtcaaacgc tcaatcatct
    33721 ccttgtgctc tggataggtc gccagtagat ctaccctttc aaataattca aaatcctcaa
    33781 attcaaaacc aggagcaaca agacaagaaa ccagagcata atccttatca actgttgatc
    33841 cccaaatagt gcccttagga acacagtagt gaagttgttg ccctttggat atgtccaggc
    33901 ctaaagtgac tgcttcgtag tgaccatctg ctgtaatcat gtgaacagta agtggggatc
    33961 ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg aaaatgtgaa ggattcgttt
    34021 cttctaataa gaaataaata ctggtataaa gcgcccttcc cttaccagca aggtttatag
    34081 tgtctgaagc tttttttgtt tgtctaaaat agccaccttc aacatgggga gctaactcta
    34141 gagttcttat caagtcttct ttatccatcg gggccaatgg gttgaagtaa ctcttgttca
    34201 aagtggtttt acgatttcaa gaactcctct cagttctgag gacacggtaa tgattgatgc
    34261 gacggaagta caaatcaatc gccctaaaaa aagaattagc gaatgattct ggtaaaaaga
    34321 aatttcacgc tatgaaggct caagcgattg tcacaagtca agggagaatt gtttctattg
    34381 ccatgatatg aagttgttca aaatgagttg cagaaatatc ggacaagctg gtaaaatctt
    34441 ggc
//
LOCUS       DBERYS010000020        33902 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.020, whole genome shotgun sequence.
ACCESSION   DBERYS010000020 DBERYS010000000
VERSION     DBERYS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.020"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..516)
                     /locus_tag="ACL8LA_001075"
                     /pseudo
     CDS             complement(1..516)
                     /locus_tag="ACL8LA_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(890..1039)
                     /locus_tag="ACL8LA_001076"
     CDS             complement(890..1039)
                     /locus_tag="ACL8LA_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGS1812844.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            1004..1123
                     /locus_tag="ACL8LA_001077"
     CDS             1004..1123
                     /locus_tag="ACL8LA_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGS1812845.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(1219..2565)
                     /gene="glnA"
                     /locus_tag="ACL8LA_001078"
     CDS             complement(1219..2565)
                     /gene="glnA"
                     /locus_tag="ACL8LA_001078"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGS1812846.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2602..2958)
                     /gene="glnR"
                     /locus_tag="ACL8LA_001079"
     CDS             complement(2602..2958)
                     /gene="glnR"
                     /locus_tag="ACL8LA_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGS1812847.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(3035..3562)
                     /locus_tag="ACL8LA_001080"
     CDS             complement(3035..3562)
                     /locus_tag="ACL8LA_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS1812848.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3698..4912)
                     /locus_tag="ACL8LA_001081"
     CDS             complement(3698..4912)
                     /locus_tag="ACL8LA_001081"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGS1812849.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFEN
                     PDFQAGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEG
                     KVLPGLVALTEK"
     gene            complement(5002..9912)
                     /locus_tag="ACL8LA_001082"
     CDS             complement(5002..9912)
                     /locus_tag="ACL8LA_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGS1812850.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYRFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            10205..11835
                     /locus_tag="ACL8LA_001083"
                     /pseudo
     CDS             10205..11835
                     /locus_tag="ACL8LA_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
     gene            complement(12394..14001)
                     /locus_tag="ACL8LA_001084"
     CDS             complement(12394..14001)
                     /locus_tag="ACL8LA_001084"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGS1812851.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDTEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(14391..14978)
                     /gene="rpoE"
                     /locus_tag="ACL8LA_001085"
     CDS             complement(14391..14978)
                     /gene="rpoE"
                     /locus_tag="ACL8LA_001085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGS1812852.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(15106..15555)
                     /locus_tag="ACL8LA_001086"
     CDS             complement(15106..15555)
                     /locus_tag="ACL8LA_001086"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812853.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(15560..15949)
                     /locus_tag="ACL8LA_001087"
     CDS             complement(15560..15949)
                     /locus_tag="ACL8LA_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGS1812854.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(15977..16627)
                     /locus_tag="ACL8LA_001088"
     CDS             complement(15977..16627)
                     /locus_tag="ACL8LA_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS1812855.1"
                     /translation="MRDKQTFLMKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIR
                     GDLPDYLTILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16617..17180)
                     /locus_tag="ACL8LA_001089"
     CDS             complement(16617..17180)
                     /locus_tag="ACL8LA_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1812856.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(17345..17579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17633..18136)
                     /locus_tag="ACL8LA_001090"
     CDS             complement(17633..18136)
                     /locus_tag="ACL8LA_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGS1812857.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(18329..19159)
                     /locus_tag="ACL8LA_001091"
     CDS             complement(18329..19159)
                     /locus_tag="ACL8LA_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1812858.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(19144..20826)
                     /locus_tag="ACL8LA_001092"
     CDS             complement(19144..20826)
                     /locus_tag="ACL8LA_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812859.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(20928..21185)
                     /locus_tag="ACL8LA_001093"
     CDS             complement(20928..21185)
                     /locus_tag="ACL8LA_001093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812860.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(21192..21740)
                     /locus_tag="ACL8LA_001094"
     CDS             complement(21192..21740)
                     /locus_tag="ACL8LA_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGS1812861.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(21755..22603)
                     /locus_tag="ACL8LA_001095"
     CDS             complement(21755..22603)
                     /locus_tag="ACL8LA_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGS1812862.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIMEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            22791..24140
                     /gene="trkA"
                     /locus_tag="ACL8LA_001096"
     CDS             22791..24140
                     /gene="trkA"
                     /locus_tag="ACL8LA_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGS1812863.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            24144..25583
                     /locus_tag="ACL8LA_001097"
     CDS             24144..25583
                     /locus_tag="ACL8LA_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS1812864.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25708..26109)
                     /locus_tag="ACL8LA_001098"
                     /pseudo
     CDS             complement(25708..26109)
                     /locus_tag="ACL8LA_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
     gene            27507..27791
                     /locus_tag="ACL8LA_001099"
     CDS             27507..27791
                     /locus_tag="ACL8LA_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812865.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            28118..28336
                     /locus_tag="ACL8LA_001100"
     CDS             28118..28336
                     /locus_tag="ACL8LA_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812866.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(28703..31027)
                     /gene="pflB"
                     /locus_tag="ACL8LA_001101"
     CDS             complement(28703..31027)
                     /gene="pflB"
                     /locus_tag="ACL8LA_001101"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGS1812867.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            31421..32482
                     /gene="dinB"
                     /locus_tag="ACL8LA_001102"
     CDS             31421..32482
                     /gene="dinB"
                     /locus_tag="ACL8LA_001102"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGS1812868.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(32629..33474)
                     /locus_tag="ACL8LA_001103"
     CDS             complement(32629..33474)
                     /locus_tag="ACL8LA_001103"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGS1812869.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(33505..33642)
                     /locus_tag="ACL8LA_001104"
     CDS             complement(33505..33642)
                     /locus_tag="ACL8LA_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812870.1"
                     /translation="MRYQTHYQLKQKLWLDPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            33729..>33902
                     /locus_tag="ACL8LA_001105"
     CDS             33729..>33902
                     /locus_tag="ACL8LA_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1812871.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKL"
BASE COUNT        10078 a         7260 c         6229 g        10335 t
ORIGIN      
        1 tattcgttgt tgttcggata gtggggggag agcaattaat aatagattaa aattataatc
       61 attgattgca ggataacttg ttccagtaga tttattatta acacgattga taaaattatc
      121 tgataataaa taatatttca aatatgtttc gttaagtaaa gtatccaaaa caataaatgc
      181 tgtactagct atcaaatact ctttaagttc tctaactaca gcaatatttt ttagatatgg
      241 tctaactgtt gaaaataaga cactattctg cgaaactaat tttctagcac gggaaggcgc
      301 ttgttcaggt gaaagatatt gtagattttt gtagttgatt atgttctttt ttctatcaat
      361 actagacgta tctatatacc taaaggattt ctctggctta ttttgcccaa aattccaata
      421 aattgatttt atcctcaccc actcccaagt atcaggaata tcataaggaa catcaatttc
      481 ttgagtactt ccatcagcaa acttcccata atgtttctta tgtgcttcaa gtatataaaa
      541 aggcgtaaaa atacgcctat agataatggg gttgaaatag gtttattgtt gatgagattg
      601 tagataattc aattttttac ttccaatcga atattcaaaa cctccacctt ttctgcctgt
      661 aattgttcat cataaaattc aatatcttca ggattttccc cttggcaacc tcggcagaaa
      721 tattcttccg ctcgatcagg attcaaaaat cgacaagcac aaacaaaaca gtcgccatca
      781 tcatttattg agataatata gtagattgaa ataagatgta aacaaatcga ttaggaaagt
      841 taaattagtt tctagaaatt tttagcagat gtagtgtact attctagtct caatttacta
      901 tggcttcaaa tatatctttc gaaaaaatat ttacagatgt gtaattttga agcttgcaaa
      961 agttagtaaa cttgtagatt tcgatttgaa gtaacttgtt ttcttgcccg atattgtttt
     1021 tgaaattgaa tttttccata gtgactcctt aattttcttc tacacgtctg atgataaatc
     1081 taattcgcaa aagagtcaag aggatttttc gaaaaataaa tagcgaccga aatcgctatt
     1141 ttaagggtta taggtatttg atggcttaga ctgctgtgtg actgtttacc cacaggcaat
     1201 ctttcttcta tattagtatt agtaaaggtc taaataatta tcaatttccc attgtgaaac
     1261 gaaggttgca taacttgccc attcgattcg tttggcttca aggaagctag tatagatgtg
     1321 atctccgaga gcagctttaa ccacttcatc ttctgtcaaa gctttcaaag cgttgtgaag
     1381 agttgatgga aggtctgtaa taccagcttc cttgcgctct tctgctgtca tgatgtagat
     1441 attttcttcg ataggagctg gtgcttcgat tttattttca ataccataca aaccaacttc
     1501 caaaagaaca gccatagcaa cgtaagggtt cgccattgga tccactgaac gcaactcaag
     1561 acgagttccc ataccacgtg aagcaggtac gcgcacaagt ggcgaacggt tacgaccagc
     1621 ccaagcaatg taaacaggcg cttcataacc tggaaccaaa cgtttgtatg agttaactgt
     1681 tgggttcatg atggcagtat agttgtaagc atgcttgatc aaaccgccta ggaaatggta
     1741 agctgtttct gacaactgca ttccttttgg atcatttgga tcaaagaagg cgttatttcc
     1801 ttctgcatca aacaaggaca tattacagtg catacctgag ccagcaatac caaattttgg
     1861 cttcgccata aatgttgcgt aaagtccgtg tttgcgagca atggttttaa caacaagttt
     1921 aaagatttga atcttatcac aagcacggag aacttcatcg tacttaaagt caatctcatg
     1981 ctgtccaacc gcaacctcgt ggtgactcgc ttctacttca aatcccattt tggtcaagac
     2041 attcacaatc tcacgacgtg tgttgtccgc aaggtcagta ggtgccaaat caaagtagcc
     2101 acccttgtca ttcacttcaa gtgttgggtc cccattttca tccaacttaa ataggaagaa
     2161 ttctggctct ggaccaaggt tgaaggattt gaatccaact tcttccatgt gacgaagagc
     2221 tcgtttcaaa ttaccacgag ggtcacccgc aaatggttca ccttctgttg tataaacatc
     2281 acagatcaga cctgcaacac ttccattttc atctccccaa gggaagactg tccatgtatc
     2341 caagtccggg tacaagtaca tatccgactc attgatacgt acaaaacctt caatagaaga
     2401 tccatcaaac ataaccttgt tcgacaagac cttatctaac tgttcatctg tagcaggaat
     2461 ttcgacgttt ttcatggttc ccaaaatatc tgagaacata agacgaataa aggtaacatt
     2521 tttttccttg acttcacgac gaatatctgc agctgtgatt ggcataagtt ttctccttaa
     2581 tctatgacta cttgcggttg cctaaccgcg accaaaaggt gactgtactg aagcaaaacg
     2641 cccctgttgg aggagttcat tgtgaagtgc acgacgtact tcagtctgac taaccgcttt
     2701 cttggatttc gcttcacgtt cagcatattt tttcttaatg gcagcgatat tataaccttc
     2761 agagatataa tctttgattt caagcagacg atccatgtca ttcaaggaat acatgcgacg
     2821 atttccttcg tttcgatcgg gcttgatcaa ctcttgattt tcataataac gaatctgacg
     2881 cgccgataga tcggtcaact tcataacact gccgatagga aaaacagcca tatttcggcg
     2941 aaattctttt tccttcattt acaatttcct tctttctgtc tattatagtc taaaaaaaga
     3001 caaacgtcaa ttaataatgt tataaaatgt aacattattt ttcttttttc tctaaaaaga
     3061 gacgaatacg atcaatatcg taatttacga taattgcgac aaaaactccc ataaatgttt
     3121 ctaatacacg cacaaacacg tacaaaattg tctcaccact tggaattgat agggtaataa
     3181 ttaacatagc tgctacacca ccaataaccc ctgctttgtt attcatggct acatttgtca
     3241 taatggttaa catggtgcag attggaacaa ctaccaaggt cacccaaaag gcttcgtgga
     3301 aaaaggtatt taataagaag aagaccaagg catagagtcc accgatacta tttcctagaa
     3361 tacgcgaagt cccaaaatga acactctcat caaaactctc cctcaggcta aaaacggctg
     3421 tcaaagcacc aatttgaaga cctttccagc caaaaaagcc aaaaatcaag agaactagaa
     3481 aaacagcaat acctgtttta aaggttcgca taccaagttt gaactgggat ttatcgaatt
     3541 tatatttttt aaaataactc ataatctcaa ctttctattt ccattttatc ataaatcggt
     3601 gatttttatg agtaatagtt gagaggaagc gtttttattt taagcaaaag aaaagaggaa
     3661 ctttcatccc tctcttcttt gatttgttta taaaatctta tttttctgtc aaggctacaa
     3721 gtcctggaag aaccttacct tcaagaagtt ccattgatgc tccaccaccc gtactaatcc
     3781 atgagaactt gtctgcacgg ccaaggttaa tcgctgcggc agctgagtca ccaccaccga
     3841 tgattgattt aactcctggt tgtttcacga tagcgtccat cacaccgatt gtaccagctt
     3901 ggaaatctgg gttttcaaat acacccatag gtccgttcca tacaactgtt ttggcaccag
     3961 tcaaagcttc gtcaaatttg gcgatagatt ttggaccgat gtcaagacca aggaagcctt
     4021 cagaaactgc ttcaccttca gaaactgctt caccttcagt gtcacgcact tcagtgtaac
     4081 cagcaaatgc gttagcttct tttgagtcaa ctggcaagat caatttacca tttgcttttt
     4141 caagaagagc tttcgcaaca tccaatttgt cttcttctac aagtgagtta ccgatttcga
     4201 taccttgtgc tttgtagaat gtgtaagtca tcccaccacc gataaggact ttatcagctt
     4261 tttcaagcaa gttttcgata acaccgatct tgtctgaaac ttttgaacca ccaaggatag
     4321 ccacgaatgg acgttctgga gtttcaactg cttcttggat gtaggcaatt tcgttttcaa
     4381 gaaggaaacc agcaactgct ttttcaacgt ttgctgagat accaacgtta gatgcgtgtg
     4441 cacggtgagc tgtaccgaat gcatcgttta cgaagatacc atctccaagt gatgcccagt
     4501 atttaccaag ttcaggatcg tttttagatt ctttcttgcc gtcaacatct tcgtaacgag
     4561 tgttttcaac caagagaact tgtccatctt caagagcgtt gattgccgct tccaattcag
     4621 caccacgagt gacacctggg aaaacaacat cttgaccaag ttttgctgcc aagtcagctg
     4681 ctacaggagc aagtgattta ccagctttat cagcttcttc tttcacacgt ccaaggtgag
     4741 agaaaagaat tgcacgtcca ccttgttcga tgatgtactt aatagttgga agagctgctg
     4801 tgatacggtt atcgttagtg attacaccat ctttcaatgg tacgttgaag tcaacacgaa
     4861 cgaggacttt tttacctttc aagtcaacgt ctttaacagt aagttttgcc atgttacaaa
     4921 aactccttta tttattttat tcgaaactat tatatcacac tttagaaata taaggaaaga
     4981 tttcacaaga tttttcgata tttaatcttt atcttctctt ttccgtctca aggtcaaggc
     5041 taaactagca agaccaaggc tggctaagcc tgctattagg gcttcattag catcagctgt
     5101 tcctgtattt ggcaattgtt tgcttgcttc ttctgattta actgctgtat tttgagctgg
     5161 tttttcctgt gtagctacta ctgctggtac tgtttttact tttgttccaa tttcaacaat
     5221 ttctggaatc gcttcttgga tgacttctgt tgaacgaaga cgacgctgac cgttttcatc
     5281 aacttcaaag acatgtctta acagtccatc tcgtccctgt atgatgactc gttgttcccc
     5341 aactagcatc tcagcatttt catgctcttg acgatgaaag gcaacctttt cctcttgaac
     5401 ttccaattta ggagcctttt gttgatcaga agccggttga taagtaggtt ttggaaggac
     5461 tgtgttttgt ggtgctggtt tagcaggttg tggtttttca gcagattcat aaacaaaggc
     5521 ataatgagta aagtgaggtg ctgtaaagat aacatgacta tccgtttgtt caaaagccaa
     5581 ttctactgcc tctttgcctt caggtaagaa aaatactttc ttaactttct tatctttttc
     5641 aattgggatt ttcacaatag aagcataaga gagatcaaca tcttgacctt tttcatccaa
     5701 accttctatt tcaaagacat gagcatcttc tccagcaaag tatttcttct cttcagcact
     5761 tgcttgaaca cgctctactt tcaaaccctt gatgacagtc ttctctttat ttgagaagtg
     5821 tacttcaaca cctgttttac tatcagaaaa gactgctggc ttgtcttctt tcaataaagc
     5881 aaccagatct tttagactct gtttcgctgt tgcaagctct tctggactca aatctacttt
     5941 ttcaaccaag gccttgtttt tctcaagaag cggagctagg gcagaacctg aaggatggtt
     6001 tggtttggca gccaagagtt cttgaacctc acctgttaaa cgatcaagct ctgtcttttc
     6061 ttccttaaat ttctcttgac cattcagagc tttatgagct tctgtcaatt tattaagtcg
     6121 gtcattgact tgatcttggg tgctagtttc atggtctttc aacacttgtt cggccgcttc
     6181 aattgctttc aagaaagctt cttttacttc tggtgaagca aagttataga ccaccttgtt
     6241 cttagcagct tcaacttctg ctaatttttg tttgaggtac tcatcgttca atgctgcttt
     6301 tggagatacc aaaacatcaa agttgacttc atgaccttgg taacgaagcg tcaaggtttg
     6361 acgaccagtc ttttgagcat cgtaaccaga aatttcaact ccctcatcag taaaggaatg
     6421 ttcttccatg gtgtcattgc tataagccac tgcaaagcgt ccttcagaca agtctaagct
     6481 atcaccaact aggtaatctt ttttcggttc ctgacttact tcaattccca aaatagtttt
     6541 cggacttgct tcgtcttggc cagtgacagt cactgacaaa ttagcattta ctggttgtcc
     6601 caaatattgg agagtaagat tctgttctcc cttatgatgc gtatcaaaac ctgatactga
     6661 tacacctgcg tgagttaggc gaatgagttc gtcctcagtt cctccttcat actgaactcg
     6721 aagaacacca cctctaaggt ccaaatcctc cccttcggcg tagcttgttt tcttaggtcc
     6781 tgtttctagg ctgactgact tgattcttct gtcatctttt ggaacggaaa ctgctaggac
     6841 attactaatt tccttgcctg gcaactgggt acgataataa agaagaccag attgatttgt
     6901 caaatccaat ggtgcacttg ctaggtctcc tccaacttcg ctcttcaagg ttgcgagtgg
     6961 agtttgagaa tttggattat cataaacggt aatagttgct ccagccggta catctgcaaa
     7021 gccaacttgt accttgttat tgcctagaga acgggctgca gccttggcca tcggaatatt
     7081 gacactctca gtatcaagct tttcatacat tttccagtta tagatacgaa tagccttcca
     7141 tggagttcca ttgtcagaag tgacaacatt caagcgccag tcttgagcag tgattggttt
     7201 atcaagagtg atatctgtca cgtgtgcttt gttgccacgg acttccttag ctagcttcca
     7261 ctcaccatct gcatctttat aataaaggtc aaagtctttg gtgttcatca agccatcgtt
     7321 aacagactca ccaccagccc ctgcatgatc catgacccat ctaacaacgg tacgtggctt
     7381 ggtcaaacga atatccacac taccactcaa ctgagctgaa gaccatttat ctgacaagct
     7441 agtaatggta ccgttcaaca taccttcaat accttctcca ccttctgtat ctgggaaggt
     7501 acttccgata acagtagccc ctggaacgat gttttctgct agaggtcttg gaagacttgt
     7561 gtcttggaca gtcatccccc actcaaaggt tgtggttgca gcttctgaac gaactccatt
     7621 tttaccgact gctacaacct tcagttcttg agttgtaccc tgagcacttg ctgagcggct
     7681 aacttttggt agataaatag ttgtagaaga tgagccagtt agtaatttcc agctgtctcc
     7741 atctttttca taaacttcat agaaatctgc atccttgttg cctttaaatt gcacaactgc
     7801 ttccgcttct tgggcatttt taagagattg tttcactaca gaaaagcttg tcggcgattg
     7861 tggctcttgg tgattgtccg agatagttaa ttgtcctagg ttgaaccgat aatcttttac
     7921 agcaccttca tgctcgaaaa atagtttgac tgcatagatg gttttacctg ctagtgagct
     7981 gagatcaaat tcttcatttg tccagttgtc agaaagggtt agctctttcc atgcatctgc
     8041 atcatcgaat ttgtagtctg gagttgtaga gaatgccata taaactttag aaccttttcc
     8101 tcccttgtgg gcaacacgaa gtttggtctt ctcagttact tctaacttag tagaataaag
     8161 tctcacatcc tgatctgtct taccggctac atcaccagag aatttaaggg aatttccgcc
     8221 attataggca tctgtaaaat catattctgc acgaagtttt tcccctgttg aagtctgcca
     8281 ccagcgccat gttggaagaa cacctgatac tgaacgataa ttccactcag aatccttaga
     8341 aaccttacca tctacgaacc attttttacc atgacctgta ttaaaagaag tagtaaaagt
     8401 attacctacc gctggcgtac ggtccgcaac taggttagca ataccatacc aatctttgtc
     8461 acctggtttt tgaccagtag ggtctccttg ataacctgta aagaagatat cttcattttt
     8521 atgataatct tcaccagttt ttcctaaact tgtaatggta tctggggcaa ataaaccaag
     8581 agaaaggcgc aatttcccat tttcgtctaa aatgtcattc cacttaacct ttgtcttgta
     8641 ggaaccaccc tgttgcaatt ccaaacctgc aaatacatca taaggattac gaccaatcca
     8701 gttggcagtt gcaatagtgt aatcattttt agccttatcc cagttaaagt tagcaaagaa
     8761 gttatctgcc ggaaccttat ctccttctgg ttgcatgaat tggtagttgt attctcccaa
     8821 accatcttga tggtaacgtc catagttata ggtcatggca tcataccaag aatacttgat
     8881 tggatggttt accttagcag catattcctt gctatagagc ataaactggc gcatcttttc
     8941 tccaagaggt ttaaccaaat ctccagttgt ttcttggttg atgaaatagc catcatagcc
     9001 ataatacttg gccatgtcta ccaatttacg ggcaattggg aagctaccat ctgcgtcttg
     9061 cttcaaagct tcagcaaatc tttcttgatc tgcaatacta ttagaccagt tgaagaagag
     9121 tgtaccgtat acaggaaccc cgttacggtg acctgcatca ataacgtcag gagttggtac
     9181 gagaccttcc cagaagacca ttgaatctag atattgccaa tagtcaaaag cataggcctt
     9241 gaactcttct ccaccaacag aagcatggtc ttttgcttta gaattggtgt ttgataaggc
     9301 ttgaactttt gcttccttgc tagctttttc attgactaaa tgacctgtcc gacgtgaagc
     9361 gaggacaaca gatccgcggt taatagcatc atcttcacga gcacctggtt cccattttaa
     9421 caattcttcc caagaattaa atttgatttc ctttggtttt aatgtttttt ctgtattttt
     9481 agggacatct ggctggactt ctttttcagt cttattagct tctgctgctg gcttagactc
     9541 atccccttgg ttagttgctt cgggctttgc ttctgtttcc ttgtcagact taggttttgc
     9601 ttctgcagat ggtgtttctg caaccacctc acctgctttt tcctctggtt tagctgcgct
     9661 agctgcttct gccttttcta gcttgttttc tctagcatgt tccccttcat tctgcttatc
     9721 agcttcattg gtcgaatgct cactctctcc tgaaacaagc tctgtattag ctccgctatt
     9781 ttcaggaact gcagtttctt cagccaaggc tggaccagca aataaaacag caccaatcat
     9841 cagcgagcag gctcctactg ataacttacg aatactgtaa cgacaacgtc tttcaaaaaa
     9901 tggattcttc attttctcct cctaaaagat tgattttgtt gttttaaaga aagcgcttta
     9961 tattctgtag ttttttatca aactctagaa ccatgataat cacattttta tcctaaaaca
    10021 tttttagaca aaaaacaata actgtttgca caaatctaaa ataaggttaa aaactcttaa
    10081 aaataataca cttatcaatt ataattttct tttacaaact tgacattaac acttgttaga
    10141 tttactttta tcctttaaaa tagaatagga gatattccgc gattattttt cggaggaaaa
    10201 agaaatgtcc attaattggc aggaaatttt atttcacttt ttaggtggtc tggggctatt
    10261 cttatatagc atcaagacca tgggagacgg tttgcaacaa gctgctggag atcgccttcg
    10321 tttttacatt gacaaatata ctagtaatcc tttctttgga gttctggttg gtattgggat
    10381 gactgctcta attcagtcta gttctggtgt aacagttatt acagtcggcc tggtcagtgc
    10441 cggtctctta accttacgtc aggctatcgg gattgtcatg ggtgctaata ttgggacaac
    10501 tgtcacatcc tttctcatcg gttttaaatt aggtaactat gccctaccta tgctctttat
    10561 cggtgccgtc tgtctctttt ttacgaaaaa tcggacagtc aataatatcg gacgcatcct
    10621 ctttggtgtc ggtggtatct tttttgccct caatctcatg agcggcgcaa tggctccact
    10681 caaggattta caggtcttta aggactatat gattgagcta agtaagaatc ctgttttggg
    10741 tgtctttgtc ggtaccggct tgaccttgct aattcaagct tcttcggcta ccattgggat
    10801 tttacaaaac ctctacgccg gcaatctaat tgacctacag ggagctttgc cagttctatt
    10861 tggtgacaat atcgggacaa ccattacagc catcattgcc tctttagggg ctaatattgc
    10921 agctaaacgg gtagcaggag ctcatgttgc cttcaacgtt atcggaacag tcgtctgcgt
    10981 tatttttcta gttcctttta ctgtcctgat tcattggttt gaagctacgc taaatctagc
    11041 accggaaatg accatcgcct ttgctcacgg aaccttaata ttaccaacac cattgtccaa
    11101 tttccattta tcggagctct ggcttacttt gtaaccaaga ttattcctgg agaggacgag
    11161 gttgtcaaat acgaaccctt atatcttgat gaacatttca tcaaacaagc cccatctatc
    11221 gctctaggaa atgctaaaaa agagctcttg cacttaggaa actacgctgc taaagccttt
    11281 gacctttcct ataagtacat cattgacttg gatgaaaaag ttgctgaaaa agggcataaa
    11341 accgaagaag caattaacac catcgatgag caattaacac gttatctcat tgccctttca
    11401 agcgaagctc tcagccaaaa agaaagtgaa gtgctcacca atatccttga ttcctcccgt
    11461 gatttggaac ggattggaga ccacacggag gctctactca atctgactga ctatcttcaa
    11521 cggaaaaatg ttgaattttc tgatgccgcc ttgaaagaat tagaggaagt ttaccgccaa
    11581 actagtgact ttatcaaaga tgctctggat agtgtggaaa acaatgatat tgaaaaagca
    11641 cgcagtcttg tagaacgtca tgaagcaatc aataagatag aacgtgttct cagaaaaacc
    11701 cacatcaaac gcctcaacaa aggcgaatgt tcaacacaag ctggggtcaa ctttatcgac
    11761 atcatctcac actacactcg tgtatcagac cacgctatga accttgctga aaaggttttt
    11821 gcagaacaaa tctaataacc aagaagctat ccatcataat tggatggctt cttacttttt
    11881 cctaagcaag actaggctga atgaaactga aagagtattc tgcagatata tagtatattg
    11941 aaactagaat agtacacctc tatttctaaa acattgttag aaatcgattt gactgtcctg
    12001 atcgatttgt cctgttatta tttcatttta ctatagtgga ttaaattaaa aaagtataat
    12061 gtattgaatc tataacagta catcttgact gctaaaatat ttctataaat taatttgact
    12121 ttccttatag agatgttcac atcttatttc aattcatgat acaatccagt tcctaaaact
    12181 ttgctagaaa ttaatttggc tttccgaatt aatttgttca gattttattt catttcaata
    12241 tactgataaa tccattcact agcacagggc aagagggagt cgctattgat ttcaacggta
    12301 gcaggtacat acagaggact cctacctcag atatactgca atatcatcgt atgcaaaaaa
    12361 cctctgagat ttttctcaaa ggttctgatt ttgctaattg ctgttctcaa ccgctgcagt
    12421 aacaaaggca gtgtagagtt cttctggtcg gtttggacgg cttgacagtt cagggtgata
    12481 ctgacaagct acaaagaatt tattttcagg aatttccacg atttctacca aacgattgtc
    12541 tggagaaact cctgaaaaga caaaacctgc tgcctcaaac tgctcacgga aggcattatt
    12601 aaactcataa cggtgacggt gacggcgttg caccacttct tgattgtgat aagcagcagc
    12661 agccttagag ccacgtttca acttagacgg ataaagtccc aaacgaaggg ttccacccat
    12721 atcctcaata tcaatctgat cacgcatgat atcaatgata gggtattttg tttctggtgc
    12781 aagctctgca gaattggcac cttcaagacc taaaacgtga cgagcaaact cgatacatgt
    12841 caactgcatt cccaagcaga ctcccaacat tggaacatca ttttcacgcg catagcggat
    12901 ggcttggatt ttcccttctg taccacgttg accaaaacca cctggtacga tgatcccgtc
    12961 cgcatcagac aagagttctg ctacattctc tgctgtcaca tcattggcat tgacccaatt
    13021 gattttaact tctgtatcat tgacatagcc agagtgtttc aaggcttcga ccactgagat
    13081 ataggcatct tgcaactcca catacttacc aacaagggaa atcttaactt gtttcttgag
    13141 gttcatgacc ttgtccacca tggctgacca ttctgtcata gccgctgctg gtgcgtctaa
    13201 tttcaaatga tcacaaacaa tttggtccat cccttgtgcc tgcaagttca gtggaatttg
    13261 gtaaaggtgt tcaacatcca acgattcgat aacggcttct ggtgccacat cacagaactg
    13321 ggccagttta tttttaattc cttgaccagc tggctcttct gtacgaataa ccaacatatt
    13381 tggttggatt cccaagccac gcaattcttt gacagagtgt tgggttggtt tggttttcat
    13441 ttcaccagca gccttgaggt aaggaagcaa ggttgtatgg atatacatga cattatccgc
    13501 acccacatct gccttcatct gacgaagagc ctctaggaat ggcaaggact cgatatctcc
    13561 tactgttcca ccaacctctg tgataatgac atcagagtcg gtcgttagag cggcacgctt
    13621 gattttttct ttcaaagcat ctgtgatatg aggaatgact tgaacagttg ccccaaggta
    13681 ttctccacgg cgttctttac gaagaacttc actgtaaatt ttcccagttg tcacgttgga
    13741 atatttgttg agattgatat cgataaaacg ttcatagtga cccaagtcca aatctgtctc
    13801 agctccgtca tctgtcacaa aaacttcccc gtgctggtaa ggactcatgg ttcccggatc
    13861 aatattgata taagggtcaa acttttgaat ggttactttg agaccacgat ttttcaagag
    13921 acggcctaga ctcgctgcca caatcccttt cccaatagac gataccacac caccagttac
    13981 aaaaatatat ttcgtagaca tagattcctc tttctaaaat gctcaaggtc ttgttaacta
    14041 cgaggggata tagaaaacaa gaccctacta ataagaataa tctgggacga ctgcaccaga
    14101 ttcacaacta atactcttcg aaaatctctt caaaccgcgt caacgtcgcc ttgccgtaga
    14161 tatgtgttcc tgactttgtc agtcttatct acaacctcaa aacagtgttt tgagcagcct
    14221 acggctagtt tcctagcttg ctctttgatt ttcattgagt ataaaataca agaagataac
    14281 ttcttgaaaa aacaaaaata gctccctaag aactagggag ccccgacctc taaaagaggt
    14341 gcccaaacaa tatgatacct aaaaatagga taattgtcaa tagctaactc ttattcctcg
    14401 ctggtttcag cgtcgtcatc tgaaaactcg tcgtcgtctt cgttgagatc cacatcttct
    14461 cccaagtcat ctggagcgat ttcgttgatt tctgcatcat aagcttccac ttcatttttt
    14521 tcatcatctg gattttcatc atcgtatgaa agagctggat ctgcttcgta tgcatcttcg
    14581 tcttctggat catctgcatt gtagtcaatg gcatctgaat caccatccat aaaggcattg
    14641 acacgttttt tcttagcttt tggtgctact tcatcgtcgt cattttcttc aagagcgatg
    14701 atttcttcgt cgatttcgtc cacaccatac catgaacgaa gaccccattt gttgtcccca
    14761 agtgagatga agctaccgtc aaagttcaac tctgtgtaga acagaggcaa agcttcgcgg
    14821 atatcgctgt ttgatgttcc aaggtagttt tgaatttcgt ttacaagatc gctaaaatgc
    14881 atctcatgat cgcgaccacg aagttctaat atagcacgcg ctacctcaat catagatagt
    14941 tcactttttt cttgcccagc aaatacttct aattccaaag cgtttctcct catttatact
    15001 actatcgcca gagcgaacag actctgacct cattttatca tttactcttt attttacgat
    15061 aattttgcgg aatagtcaaa ggttaagggg gagaaagtgg caggattaga ctaattccaa
    15121 tataaaactc cttccttttt ctgttgctcc attttccaca aatccaagcg acttgaaaca
    15181 cctcctagaa gcatgattgt aggtgtagat ttccttgact ctcaattctt tccatccttt
    15241 tactcgagcc aattcaatca aagcacttag aatctttttt ccaagtcctc gatgttggta
    15301 agcggaattc ccaatcacaa tggggagatt atcctgagat agtgtaatat ccccaattgg
    15361 aaaccattct cccttctcct tgacttcaat ccaaaaaagc tcaccatgcc gatccaaata
    15421 ggaatacatg gcttccaagg tcgcttgact gtaaggaagc ttcaccccat ctacgaggta
    15481 aaccaagttc acatcctgat accaagcaaa agcttcctca caatgattga ccttataata
    15541 aggaacaaaa cacaatgcac taccttttca acaaaagagt tgctgcttga ttaaaaccat
    15601 cacaccagtt ataccatttt gcttcatacc catcttgagc taggatacga tcttctaaat
    15661 caaaaacaga gtaaatcttt ctttcctcgc aagcttgcgc atagagatga tatagttcat
    15721 caccaccatc tctatcccac tcagcagaaa tcgtatcccg atctgccaat aaagtctgat
    15781 aagccctgtg atgcccatct gtaatcagca aacaatctcc aaaggcaaga atactgattg
    15841 gatcgacttg gattgtttct gccgactggt aaagcatctg aatatcttgc aacttctttt
    15901 ctgataaata tagttgagtc ggatgaagat ctgctatgtt gactttcatt tctttctcct
    15961 caagggaatt cgatactcac ttctgtttgc ctttaaatcg ccattggaag cggagcttgt
    16021 catagaaggg aaactcgata aacaggactc ccaagcccac acagagactg gcaaggacgt
    16081 ctgatgggta atgaactccc agatagactc ttgataccag cacactgact aggtagaggc
    16141 caaggacgat ttgtacgatt tttctccaga ctggatcttt aatccgctga ctaagaataa
    16201 caatcaaagt acctaccatc aaggttacag ccagagaatg cccacttggg aaggaaaatc
    16261 ccttctcctc caccagatgt aagatatctg gccgtgggcg ctggtagata tttttaaagg
    16321 tcacgattaa aagacctgcc aaagccagat ttcccagcat gaagaaactt tctatcttcc
    16381 atcgcttacg ataaaagaca aaagctgtaa tgacaaccca agtgataatc actgggatat
    16441 caatcagacg tgtgagggct cgaaaaagaa tagtcaaata atctggtaag tctcctcgaa
    16501 tggcagtctg aatcgattgg tcaaaattga ccagcgtttc agggtaaaat ttgaccatgt
    16561 agccaagaat aacgaaaagt aaaagggcaa aactgccctt cattaaaaat gtttgtttat
    16621 ctctcataat gttttaaggt tggtttcaag agaacataca acaaccagaa tgaaacggaa
    16681 aagataacac cttcaatcaa gttaaaaggt aataccatgg tcattaggta gttggaaagt
    16741 cccaaaattt ttccaatatc aaagttagca aacttagcgt acaaaggaac agcataaaca
    16801 tagttgagaa ccaacatggc caaggttaaa ccaatagttc cagctagaga gcctagtagg
    16861 aaacgaaggg ttgtccgttc ctttttccaa atcaaagcaa atacgatgac aaaaactccc
    16921 aaagctacga tattcatcgg caaaccaatg taagtattca ctccttggct gttaagaagc
    16981 aacttcaaga gtgagcgaag caagagcact cctagagcag caggcaaatc catgaccacc
    17041 agacccacaa ggactggcaa gatactaaat tcgatcttga ggaaagatgc cgctggtaaa
    17101 agcggaaaat caaagtacat cagcacaaat gagatggctg atagaattgc aatggtcgaa
    17161 agtcgacgtg tgtttgtcat aacaggttcc tccaattttc tataaaatca gaagaagttg
    17221 gaaaggattc ctctatctat tctcactttt tatatcccaa aagttccctc ttactctatt
    17281 aaagaaaaac aaagcaagtg gttacaatcc ggctataaat ctatcaaaac agacaaagct
    17341 attctttcgt cttctcccat ccagactata ctgtcggttg tggaatctca ccacatcagc
    17401 ttgcgctcgc ggacttcttt aaagggaaag aaatagagat ttttctttta gactaggcga
    17461 caagctgtag gcataacttc tggttaagca aagcttgtca acaaagtata aaaggaaaag
    17521 atcatttcct agttaaagtc accgccggtc gggaatcaca ccctgccctg aagactcttt
    17581 tatcataaca aaaaacgctt gcaagcgcaa gcattttgtt cattttcatt ttttatttca
    17641 atttatccac ttcataggta tggacgagct caagacctgc aaagttctgc tggcgaagag
    17701 cctcatagac aatcatgcag acggtattag acacattgag actgcggaca tgttcatcat
    17761 tcatgggaat acggagagcc ttctcaggat gttctcgcat aaaatcttca ggtaagccct
    17821 tgtcttcacg tccaaagaga aaataatggt cctcgtcagt cgataaatcc acctcagaat
    17881 agattttttc cgcgaatttc gaaatcagat agagttttcc cttcatctga gatatgaaat
    17941 cttctaaact ctcgtaaaaa taaatctcta gcttatccca gtaatccaat ccagcccgct
    18001 tcatcttgcg gtcatcaata ggaaagccca taggcttgat gatgtggagg ggagaattgg
    18061 tcgcagcgca agtacgcgcg atattgcctg tattttgtgg aatttgaggt tcaaataata
    18121 caatgtgatt tgtcatgact tgcttccttt caccattgca aaaaaatagc cacactgccc
    18181 ggagtcaagc tcagcaaaca gcgtggttaa ggcatcgtta acttacctca caacaggttt
    18241 gaagtaaatc agcgaaacta ctttcttagt ataacacttt cagaatcatt gtcaatagaa
    18301 atgacttgat tttttcaatt ttttcaagct atttccaagg gttgtaaaat cgtccctgat
    18361 tctgcaagat aagtagtaaa ctaactacta aaaacaaggt tgccaagagc aaggtaatat
    18421 agtctccttt tttcaaggcc tgataactat accatgtgcg ttttttctct ttcccaaagc
    18481 ggcgaagctc catggcagtc gcaatggtat caatgcgttc tagcgagcta aaaatcaagg
    18541 gcgtaataat gagcagattg cctttgattc gttgcataag agaagctttc ttggataatt
    18601 ccatcccacg cgcctcctga gacatcttga tagtaaagaa ttcttcctgc aaatctggaa
    18661 tatagcgcaa ggtcaggctg acagaataag caatcttata gggcacacca atttgattta
    18721 aactggaagc aaactgacta ggatgggttg tcatcaaaaa gataatagcc agaggaatgg
    18781 tgcaaagata cttaatggcc aaatttagca gataaaagag ctcctggctg gttagagtgt
    18841 agacaccgat tccctgccaa atcacacttc tctctccata aagtccaacc ccatactcgg
    18901 gagaaaagag atagaccatc aaaacgttta aaacggcaaa taccgtcgca aaaacggcta
    18961 caaaggaaac atctttaaag cgaatttctg ataaatagag gagaaagact gaaaagatgg
    19021 caatcagcaa gagcattctg gtatcatagc taatcatggc cgccaatgat accagaatga
    19081 aaaagagaag tttcccagct cctgacaagc gatgaatcac agtatctcta tgctggtaac
    19141 cgattaattt agcttgcatc cctctctcct ttctttgtaa aatgccgtta aatccagtgg
    19201 atccacatct agtttcttag ccaagttaaa gatggaggtt tcttttagat tggcttttac
    19261 taacagctca ggatcgctca acagactggc tggaacagta tcggcaatca attctccatc
    19321 caccatgaca aggacccggt ctgaataatc cagcatcaat tgcatatcat gggtaatcat
    19381 gacaatggta tgcccttttt gatgtaactc ttcgagaaat tccataatct cagtatagtt
    19441 cttctgatct tgacctgcag tcggttcatc taggagaata atttcagctc ctaagaccaa
    19501 aattgaagca atggtgacac gttttttctg accaaatgac agggcagaaa taggccaatt
    19561 acggaattca taaagtccac agattttcaa ggtttcatag actctcgttt caatttcctt
    19621 ctcatccaca cctcgcaaac ggagccctag agccacctca tcaaaaatca tattggttga
    19681 aatcatttga ttaggatttt gtagcacata gcctactcgt tccgcccgct ctgcaacaga
    19741 atcgcctttt atatcctgtt tttcccaaag atagcgtcct tccgtctgaa taaagctact
    19801 tatagccttg gctagagttg atttccctgc tccatttttt ccgacaatag caatcttttc
    19861 acccttttta atatctaaat gtagggattt taaaatcggt ctatcatcat aagagaaaga
    19921 cacgtcctct aatttaaaga gtgactgcaa ttctggagtt tcttttgcca gttcattctg
    19981 cagctgaacc tgaccttttg agatagacat gttatccaga ttcgctaact gttcttcctt
    20041 gactaagtcc acacctaatt gacggagagt cgttagataa aggggttctc gaattccatt
    20101 ttgagtcaat aaatcagtcg caagcaactg gtcagggctc ccattaaaaa ggatacgacc
    20161 atcgcttatc aagacaatcc gatccacagg gcgatgcaga acatcctcca aacggtgctc
    20221 gataatcaga gtcgtcgtcc cctcttcctt atgaatctgg tcaatcaatt cgataatatc
    20281 ctgacctgac ttgggatcta gattggcgag tggctcatca aacaagagaa tcggactttc
    20341 atcaatcaag acaccagcca gactgactcg ctgcttttgt ccacctgaca aatcctgagg
    20401 acgctgatcc agtaaaggaa gaaggtccag cttttcagcc catttataaa cacgaccttt
    20461 catctcatct agggctgtca catcattttc cagagcaaac gccaaatctt ctgccacaga
    20521 caagccaata aactgcccat ctgtatcctg caaaactgtg ctaaccagat gagacttatc
    20581 atagatgctc atatcaaagg ctacttgacc ctttatcaaa aattctccat atgtctgacc
    20641 cttgtaaata ttgggaataa tcccattcaa acactgaccc aaggtagatt tacctgaccc
    20701 agatggtcca acaattaaga ctttctctcc cttgtaaatg gtcaagtcta tcccttgcaa
    20761 ggtcggttct tgttgtgttt cataccggaa agagaaatcc ttccactcaa ttatagcttc
    20821 tttcatctta ctctcttcat tcgcttctta gacttctatt ttatcataaa tcaagccctt
    20881 cttgcagtct cttctcttaa aatcttagcg ccaaaaagat tcctatccta gcttacttgc
    20941 ctaactaatc tataaacatc gaaaaagact agttgcccag ccttccccat cattttatac
    21001 tcttcgaaaa tctcttcaaa ccacgtcagc ttcgccttgc cgtaggtatg gttactgact
    21061 tcgtcagttt catctacaac ctcaaaacca tgttttgagc tgacttcgtc agttctatcc
    21121 acaatctcaa aacactgttt tgagcaacct gcggctaact tcctagtttg ctctttgatt
    21181 ttcattgagt attagtcctt tttcaaactt cctgcacgag tttgggttcc tgcataggca
    21241 agtaagagaa gagttcctgc aatagctaca gatacaccat tggcaattcc cgcaacaatc
    21301 ccttgtgcaa atactttttc tgccgcttct tgataaatca caacatctcc aagtggtgcc
    21361 aagacacccc aaacaagggc atttgcaagt agttgaatga ggttaaaaat aagaatatct
    21421 ttccagtcaa aaacaccatt gatcactcga acgtactttc taaaaagtcc cacaactaga
    21481 ccaaagagtc cgctagcgat aatccaagtc caccatagac cataaccagc aagagagtcc
    21541 ttgattgcat gaccaatcaa cccgacaagc aaaccgataa tcggtccaaa aataatagaa
    21601 agtagcgctt gtaccgcata ctgaagctgg atgcttgtat ttggaacagg ggttggaatg
    21661 ttgatcatcc cgatgacgac aaagagggca gcgccaattc cgacagcaac aacttgttta
    21721 attgtaaatt tgatttccat actattctcc tattttatcc ttctattttc tttatttcaa
    21781 tggtccaaga tgaaccgaca cctacattat aggccttggc aaaggaacct tggttgatag
    21841 ccaaacctaa acgatagaga gagttgatgt aaaggatggg ttgcccaatt ctcacatctg
    21901 caaatgattt gccatagaca acctgatttt gatagaccag catatcagca tgatagatgg
    21961 tcacttcaaa acgatcacca aattctggtt ccagcttgta aaattcttcc cgtgtgatag
    22021 aggtccaaag cgaaccgaaa cgcacatcca gaatatcaat ggctcccttc accagatgat
    22081 cttccatgat ggtcgctacg actggaagct ctacaatctg ttccacactg agctctggcc
    22141 ctacttcctc aaaagtaatg tgaccactgg ccagtttagc accagtatag gcatagacat
    22201 cacgaccgtg gaaggtataa gaatgctctg tgttttgacg cctattggcc acctcagaaa
    22261 tctcacgaat ggctacaatg ccaacgtgtt tcttgataaa ggaaagcgtc ccattatctg
    22321 gcgtgacaat gtattgattt tttgcagtct tggcaactac actcttacgt ttcgaaccga
    22381 cacctggatc gacaaccgat acaaacgtcg ttccctcagg ccagtaatcc accgtctgaa
    22441 agagacgata gctcccctca aaaatattat aaggcgtgat atcgtgcgtc aagtgatgga
    22501 tttttaaggt tggagactct tctaaagcca ctccaatcat agccgacacc gcaccatcaa
    22561 ccagaccaaa gtctgattgt aataccagta aattattgtt cattttatct ccttgtatat
    22621 cctttatcat accatatttc agagaaagtc gctaatagct atttcaattg gttagggtat
    22681 agttttacct tatagcgaat tttcactaaa agctcttgtt attagctagt aggtgcattt
    22741 tttcttgaaa aagggtatga tagttacatc atgaaaaagt aggttttatt atgaaaatta
    22801 tccttgtcgg agggggaaaa gttggttttg ccctctgtcg ctccttggtt gcagaaaagc
    22861 atgatgtttt gctgattgag caagacgaag ctgttctcaa tcatattgtc agtcgctttg
    22921 atatcattgg tatccttggt aacggggccg attttgccat tcttgagcaa gccagcgtcc
    22981 aagattgtga tatctttatc gccctgactg agcacgatga agtcaacatg attgcagcag
    23041 ttctagccaa gaaaatggga gctaaagaaa ctatcgttcg ggtgcggaac cctgaatatt
    23101 ctaactctta tttcaaggaa aagaatattc tcggtttttc tcttatcgtt aatcctgagc
    23161 tcttggctgc ccgcgctatc gcgaatatca ttgacttccc caacgccctg tctgtcgaac
    23221 gctttgctgg tggacgcgtt agcctcatgg aatttgtcgt caagtccacc agcggtcttt
    23281 gccaaatgcc catttctgat tttcgtaaaa aatttggtaa tgtcattgtc tgtgcgatag
    23341 agagggatca tcaaattatc attccaagcg gtgacatgac tgtacaggat aaagatagaa
    23401 tctttgtcac tggtaaccgt gtcgatatga tactcttcca taattatttt aaatcacgcg
    23461 ccgtgaagag ccttctcatc gttggagcag gtagaattgc ctattatcta cttggtattc
    23521 tcaaagatag tcgtatcgat acaaaagtca ttgaaatcaa tcctgaaatc gccagcttct
    23581 ttagcgagaa attcccaaat ctctacatcg ttcaaggaga tggaaccgca aaagatatcc
    23641 tgctggaaga aagtgctcaa cactatgatg ccgttgcgac tctaacagga gtcgatgagg
    23701 aaaatctgat tacatctatg ttccttgaca gggtaggtgt acagaaaaat attactaagg
    23761 tcaatcgtac cagtctcctc gagattatca atgcgcctga tttttcaagt atcatcacac
    23821 ctaaaagcat cgctgtagat acgattatgc actttattcg tggtcgagtt aatgcccagt
    23881 attcagacct tcaagccatg caccatctag ccaatggcca aatcgaaacc ctgcaattcc
    23941 atatcaagga agccaataaa atgactgcca aacctctttc tcaactgaaa ttgaaaaaag
    24001 gggttcttat tgcagccatt attcgaaagg gcaagactat tttcccaact ggggaggata
    24061 tgttggaagt tggagacaag ctcctagtaa caaccttgtt gccaaacatc accaagattt
    24121 atgacttgat tgcgaggtaa gaaatgaata aaagtatgat tcgttacctc ctttcaaagt
    24181 tacttttgat tgaagctgtt ctcctcttgg ttcctgtggc tatcgctgtc tattaccgtg
    24241 aatcgagcca agtctttaca gccctctttt cgaccatagg gattctcgta ttgttaggcg
    24301 gttcagggag tttacagaag ccaaaaaatc aacggattta tgccaaggag ggagtcttga
    24361 tcgttgccct ctgttggatc ctttggtctt tctttggtgg tctccccttt gtcttttctg
    24421 ggcaaatccc tagccttatt gatgcctttt ttgaaattag ttctgggttt acaactactg
    24481 gagcaagtat tttgaacgac atttcggttc tcagccgttc cctcctcttc tggcgaagtt
    24541 ttacccactt gattggaggg atgggagtgc ttgtttttgc acttgctatt atggacaatg
    24601 ccaaaaatag tcacctagaa gtaatgaagg ctgaggttcc tggtcctgtc tttggtaagg
    24661 ttgtatccaa attaaaaaac actgcccaga tcctctatct cctttacata gccctctttt
    24721 ccctctttgt catcatctat tatctagccg gtatgcctct ctacgatagt tttgtcattg
    24781 ctatggggac agcgggaact ggaggattta ccgtctataa cgatggaatt gcccactatg
    24841 gtagctcact gattacctat ctggttagta taggagtttt ggtttttggg gtaaatttca
    24901 atctctacta ctaccttatg ctccgtcggg ttaaggcctt ctttggagat gaagagcttc
    24961 gggcttactt ggtcatcgtg ctggtttcta caggcttgat tagcctcaat actctctacc
    25021 tctacccagg gttttcaaag agctttgaaa tgaccttctt ccaggtttcc aacatcatta
    25081 caacaactgg ttttggatat ggagatatta ccaactggcc cctcttctcc cagtttatcc
    25141 tccttttcct catggcaatc ggtggctctg ctggatcaac tgcaggtgga ctcaagatta
    25201 ttcgaggcct catcctttca aaaattgcca aaaaccaaat tttgtcaatc ctatcgcccc
    25261 accgtgtttt gaccctccat gttaataaaa tggtgattga caaagatacc cagcataaga
    25321 ttctcaagta ctttgtcatc tatgctatga ttttgctagc gcttatcttt attgtcagcc
    25381 tagatagcaa tgattttctg gtcgtgacca gcgctgtctt tagctgtttc aataatatcg
    25441 ggcctattct aggaaccact tctagtttct caatctttag tcccatctca aaaattctcc
    25501 tctcctttgc aatgattgca gggcgcttgg agatttaccc aatcctcctt ctctttatga
    25561 aaagaacttg gtctaagaga taaaatacaa aatccctctt tttacaattg tgaaagaggg
    25621 atttttatat taaaaagggg gaacgttttt aaatccaagc ctgtctgtaa gcaaagttct
    25681 tactcaaata aaagatggaa aaacttacta cacagcttgg taatgctcca atacaaattg
    25741 taaggcttta cttggattct ttgttaaatg aatatattcc attcctctgg tagcaactat
    25801 ctgaatacct aatctttctg catccacctt catctctcta taataactcc gaacttgtgg
    25861 agccagaata attaaatcat aaacacccat aatatcataa tgagctccgt acgctcctga
    25921 attcgcaatc actctaactt ctgctaattg agcgccctcg ttaattgcat tggctaatta
    25981 cgcacttgtt ccagaccctg cacaaagaac cagtactttc aactccttac tttttattac
    26041 atctatctca ccaggcatta tttcacttgt atcctcctct aaaataatat catttgagct
    26101 tgcaatatct acaacctgta gtagatatct cgtatttaga caatatgaaa acaagacgac
    26161 ttccatatag gaaaccgcct tctctctatg ttgagtgatt tatattaaaa taacttttct
    26221 tctagctgca ttttattatt ataaaaacat tcatcataac cctgaactta aataacgatt
    26281 tttattcaag atacatactc ctagaataaa ctttatatga aattctcatt tttgttttta
    26341 caattctcct tagttaaatc ttgtttaata tatgttttac atatagtatt tagcgccaca
    26401 tagtactgaa ctctctccaa aaacggttat tcctctttga atagggcgtt atcacaagaa
    26461 aagcatctcc acgtttcaac ttcatatggc tcaaaaacaa tcaattgatg ctaaaacctg
    26521 tacctagatg tttcggttca taaaaccatg aaactgtaaa agtgaatgaa attgatagcg
    26581 atagtcaaat caagaggcat cataactcta aaaagtcaca atatataagt tcatcctcgg
    26641 aaaaatatca ttctaattgt tgaaatgcct acatgaaaag aaacgtcaaa tgctcatgaa
    26701 acaacgaata caggtatcaa aactatgaca aaacaagtcc ctaaatttac taaagacact
    26761 gctcaacttt acacctgtaa gtggttgttg tataataaag ttacaaagat gtacgaccac
    26821 actgttgtaa atcatagtgt tcgcgaatat attactgata gcatttctac aaatacaatt
    26881 gaagagactg gatagacttt aaacgaaata tgtcagtgtt ttggcattcc tagccttcat
    26941 atcatttaaa gaattctata gacaaaattt ttcccaatac agacactcgt aacaactgct
    27001 tcatttttct accaacatat ttaggaacag gataagatac aagaggatta atccatagat
    27061 cagttctata ccaatctaag acaaataagc taaaaaaacg attgataata agcaaataga
    27121 ttccaaattt tctctatctg ctcattttaa taaacaatac tagtgtaact atccttccag
    27181 tcagaaactt gtcaaatcac accgaaaact cttctaaaat ttatctcgtt aggcaatcaa
    27241 gcaaaaactc gacgatagta caaacattat catacagaat tgactgccta aattatatac
    27301 tttagtaagg ttttcggata agaaaaaagg ttcattttac atttctaaac attcttttct
    27361 aagatgaaaa acagaatttt tcgattgtga tttaaagcaa caagaagatt ttcagtatca
    27421 tcctatagat acgagctaat taagaaaaac tacatttttg aatataaact acaataatat
    27481 aaactaaatt ttataggagg aagacaatgg attggtacga ttatatgata caggcatcca
    27541 aacaatcaca attcaacgca agccattggt ttcgctattt gcgaaaagtt atttttgaag
    27601 actattctta tttaacaaac caagatgtag aaaagttgct agactccaaa gaactaaccc
    27661 gttttcaaaa aattagcttg aagtatgcct ttcaagagca tactccaact cataaatatg
    27721 tgatttcatt aaataaacct gctaagttaa ccaatgttca aaaattgatg gagaaataca
    27781 aacatggata aaatgaaacc ggtcttccaa gccctaaata agaaattaat tcaggaaaat
    27841 ctgactttaa caattatggt tatgtcttag aatatcatgg tttacgtacc acacaagatg
    27901 ttgatgcttt ttatgaccaa aatcagaaaa tcaatgaaat tattgctcgt gtaggaaaac
    27961 agtttaatct taacatccac gaagagttat ggctcaacaa tcatgttgca aagcaaatat
    28021 gaacaaacaa cctcccttaa gtttatgcga atccttgtac tcatttgaga atctgaccgt
    28081 tttagtagtc cctattgagt atgtgttagg aatgaagatg atgagtatca gagagcagga
    28141 tttgcaagat attggtgcaa ttattaaata caaaaacttt cattcaccat tcgatacctt
    28201 taaatattta aaagacatgg gatttgatac tattgacctc tctgttctat tagaaggttt
    28261 tagttatgct tatggtatgg attggttaga aaaatttttt aaagaaaatc aagataaact
    28321 tcgcgaattt tattaacagt atcaaacaag atgattgtca attagccagt catcttattt
    28381 ttatgatgaa atgaaataaa atcggaacaa atcacttaga gaattccagt caatttctag
    28441 cactgtttga gccgtttcag cggactatag taaaatgaaa taagaatagg acaaatcgat
    28501 caggacagtc aaatcgattt ctaagaatgt tttagaagta aaggtgtact attctagttt
    28561 caatctatta tatttcagat tcggtccact attccttccc aaaaacattg catattgcgc
    28621 cgaaggaatt gctcatacta tcccaagcac aaaaccctca gtcgtttgac cgagggttcc
    28681 ttttcattat tcaaaaactt gattagctca atgcatccaa ggcatcatcc attgaaagaa
    28741 cttcgtggaa gacacgttgt gtcaattcag ttttttgttc tggagtgagg tatttagtat
    28801 ttacacagta tccagagata cgtacgataa cgtcttcacc tgacataatt ttttcgtaaa
    28861 catcgttcaa gtccataacg ttcaagttaa cgtgttgtcc accgttttcg aagtaaccat
    28921 caaggattgt taccaagtta tcaacttgtt catcacgagt cttaccaaga gcgcgaggtg
    28981 atacttgtgt agtcaatgag ataccgtcag ctgcataact aaagtcaagg ctagaaagtg
    29041 agttcaagtt ttgcaaccaa ccacctttag ctttgttaga tgggttagca cctggtgaga
    29101 agaattcaag tttagacaag ttcacagaac catcttcgtt gaggtataca cctttgtgaa
    29161 ctggtgagtt accagtttgt ttagagtaag caacgttaga tgtgattgtc aaaagtgata
    29221 ctgtagcttc tgcgtctttg tatagtttgt ggctacgtag acgagttgtg taagcttcga
    29281 tcaaccattc tgccaattcg tttgaacgtg ggtcatcttc accccagcgt gggtagtcac
    29341 cgattgtttc gtaatcgtag atgtagccat cttcgtcacg gattggttta actgtagcgt
    29401 atttgatagc tgacaatgta tcaacagtgt tagcaaatcc acagataccg aatcccatgt
    29461 tggcacgttg tttagttggc aagaaggcca tttgaacagc ttcgtagttg tacctatcag
    29521 tcatgtagtg gatgatgttc aaggcatcta cgtaagtgtc agtcaaccag tcaagagatt
    29581 tttcaaagtt cgctttaact gattcgaatt caagaacttc gtcacggatt ggttcgatat
    29641 caaatacttt gtagtctttg tgaacatcgt cgtaaccacc attcaaacca gtaagaaggg
    29701 ctttaagaac gtttacacga gcaccgaagt actggatgtt gtggcgttgt tcttcatttt
    29761 ctggatcaag tggagacaca cagcatgaga tacagctcat ttcaccatat ccgtctttag
    29821 ccattgttgt tacaccttcg tattggatag aagagtgttt gtggctcatg tgcatacagt
    29881 agcgacggaa gttgtatggc aatttgtcag tccaaagaac tgtcaagttt ggttctggtg
    29941 agttaccgat gttgtcaaga gtgttcaaga aacggtagtc catcttagta acacggtgac
    30001 gaccgtcgtt acccatacca gccatagaag ttgtgataaa ggttgggtca cctgagtaca
    30061 attggtcata agcttttgta cgagcaaatt taactgtacg aagtttcata acgaaatcat
    30121 caacgaattc ttggatttct gattcagtaa atgtaccacg agcaaggtca cgttctgcaa
    30181 agatgtccaa tacgattggt acacgaccta gagatgtagc agcaccgtta atcacacggc
    30241 agacagccat gaaagcaatg ttaacccatt ggattgcttc tttcacgttc atcgctggtt
    30301 tgcgaacatc aaccccgtaa aggtcaccca ggcgaacaac ttgttgcaat gcttggtatt
    30361 gaaggtttac ttcttcacga agacggattg tttcttcatc gatttctttg attgcattcc
    30421 agtcatttac tttttcttgc atcaagtagt ctgcaccgta aagagcaaga cgtgcgtaaa
    30481 caccgatgat acgtccgcgt gagtatgcat ctggaagacc agttacagtg tgtgcgtgac
    30541 gagcgcgacg aatatttgaa gtgtaggcac ggaaaatacc gtcgttaact gttgttacat
    30601 atttagtgaa gatttcgtga acagctgggt ctggttcgta tccattttct ttcaaagtag
    30661 tttcagccat acggatacca ccttttggca tgaagttcaa tttgaagagt tcatcgtttt
    30721 ggataccgaa gataacttca ttttctttgt cgataaatcc agcagggata tcagcgatag
    30781 atgttggacg agtgtccatt gggaaacgag tttcttcgta gtgtgcttta gtttcttcta
    30841 caattttctt gatgtgaagt gaacgctctg ttggtcctgc aaggaagctt tcgtctccat
    30901 cataaggtgt gtagttagct tgtacaaagc gtgatacact tgctttttct ttccaatcta
    30961 cgcctttgaa gccttcccaa gctttgtcaa aaatatcttg tgcttcaaca actgtcttaa
    31021 caaccatatt aatgtcctct tttttctagt aacagtcatc tgttacattc atgagacaag
    31081 tataccatat agtaaccgat tttaacaagt gaaaaatccc tatttttaca ctttcttttc
    31141 taaaacagtc tatattttat ttcaaactgt attatatttt tgaaaaaata aagtcctttt
    31201 ttcttttttt cagaaaaaag ggtataataa aagaaaataa gcagtaacac tcaatggaaa
    31261 tcgaaaagca aactaggaag ctagccgcag gctgctcaaa gcactgcttt gaggttgtaa
    31321 atagaactga cgaagtcagc tcaaaatact gttttgaggt tgcagatgga agctgacgtg
    31381 gtttgaagag attttcgaaa agtataaaaa ggtgctaggc atgttgattt ttcctttgct
    31441 aaatgatttg tcaagaaaaa tcatccatat tgacatggat gccttttttg ctgcggtgga
    31501 aatcaaggat aatcctaaac tcagaggaaa acctgtcatt attggaagcg accctcggca
    31561 aacaggtgga cggggagtcg tttctacctg tagctatgag gctcgagctt ttggtgtcca
    31621 ttctgccatg agttccaagg aagcttatga acgttgtccc caggccgtct ttatctcagg
    31681 aaattatgag aaatacaaag ctgtgggact ccagattcga gctattttta agcgctatac
    31741 agatttgatt gaacccatga gcattgacga agcctatttg gatgtgacag aaaataaact
    31801 cggtatcaag tcagcggtca aaattgctcg cctcattcaa aaagatatct ggcaagaact
    31861 ccatctaact gcttctgccg gtatttccta taacaagttc ttagctaaaa tggcgagtga
    31921 ttatcaaaaa ccacatggtt tgacagtgat tctacctgaa caggctgagg attttctcaa
    31981 acaaatggat atttccaaat ttcatggagt aggaaaaaag acagtagaac gtcttcatca
    32041 aatgggcgtt tttactggtg ctgatttact tgaagttcct gaggtaaccc taatagaccg
    32101 ttttggtaga ctaggctatg atctgtatcg aaaggctcgt ggcattcaca actctccagt
    32161 caaatccaat cgcatccgta aatcaatcgg caaggagaaa acctacggga agattctcct
    32221 tgctgaggaa gatatcaaaa aagagctgac tcttctatca gaaaaagtcg ctctcaatct
    32281 acatcaacaa gaaaaagctg gaaaaattgt cattttgaaa atccgctacg aggacttttc
    32341 aactcttacc aaacgaaaaa gtcttgctca aaaaacacaa gatgctagtc agataagcca
    32401 aatagccctg caactctatg aagaattaag caagaaagaa agaggtgtcc gcctattggg
    32461 gattaccatg actggatttt aaagctcaat gaaaatcaaa gagcaaacta ggaggctagc
    32521 cgcaggctgc tcaaaacact gttttgaggt tgcagataaa gctgacgcgg tttgaagaga
    32581 ttttcgaaga gtataaaacc ttgaagagtt gccccttcaa ggtttttctt atacaaataa
    32641 acggacaaaa ctgtaaagta gcaaaacact accaagcacg atacggtatt taccaaaaag
    32701 ggtgaagtcg tgttttttca catagctggt caagaagcga atagccacca tgctgaccgc
    32761 aaaagctact cccatcgcga ccaagagcaa aaacaattgc ccaaagctca agagttctcc
    32821 ggctttcaca aatttgaaaa tctttaaggc actagctcca aacataacag gaatcccaag
    32881 atagaaggta aattctgtca caactgaacg actggttcca tttaacaaac caccgacaat
    32941 cgttgcacct gaacggctag tccctggtaa aagagcaaga acttggaaga gtccgatata
    33001 gaaagcggtc gtataaggaa gcttgtccaa ctctgttaca cttggctcga tagcacgcgc
    33061 tttattgcgc ttttccaaat agatgaaggc aaccccgtag ataatcaaca tgagagcaac
    33121 tgaaaccatg ttatggaagt gggtatcaaa ccaatcatca aatttaaaga caccaagtaa
    33181 aggtaaagta gcaatcaaga ccttcaacca tagtctccaa gtcttacgaa cttcctgttt
    33241 gtccttagtc ggtttaaaag gattgagctt gttaaaataa atcaccataa ctgctaaaat
    33301 agcaccaagc tgaatcacga cattaaacat ggacataaag gcttcatttt gattttggta
    33361 ttggatgaat tcctctgcta aaatcaagtg acctgtactg gaaatcggca accattccgt
    33421 aattccttca acaatcccga agaagataga ttttaaaatt tcaataagat acatagatta
    33481 ctccctatat tataattagc gcagttagct actgccaaag cccaagtggt atacttggaa
    33541 taagccactg tggattagtt catttcctcc aatcgcttga cgatttccga aaggatctaa
    33601 ccacagcttt tgtttcaatt ggtaatgagt ttgataacgc atagacgttt tatctcgtct
    33661 aaggtaacaa ggcaaaaagt tcgtggataa ccaagtagac ttgaaatact atatctggag
    33721 gtactcccat gctttatgtg ggcattgata tcgctaaaaa taaacacgat gttacagcct
    33781 tgaatgttcc aggaaaaact gttcttaaac cactcacttt ttcaaataat aaagctggtt
    33841 ttgaactctt agatctgtct cttcgacagc tcaaccaaga ctgtctcatc gctcttaaac
    33901 tt
//
LOCUS       DBERYS010000021        32437 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.021, whole genome shotgun sequence.
ACCESSION   DBERYS010000021 DBERYS010000000
VERSION     DBERYS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.021"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..2788
                     /locus_tag="ACL8LA_001106"
     CDS             <1..2788
                     /locus_tag="ACL8LA_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1812872.1"
                     /translation="NQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGED
                     GKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGQNGN
                     PPHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVY
                     QEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTT
                     VFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKD
                     GRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKD
                     IYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENP
                     DGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIK
                     DGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDG
                     SHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTETIIKDGKSP
                     VATVRDNQDGTYTIRVENGNGTVSETTVRDGKSPTAKVVDNGDGTHTITVVNSDGTTT
                     TTTVRDGREPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTL
                     TIVDSDGREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGK
                     DGKSPKVSVEDNGDGSHTITIINSDGTVTKTVIKDGKDGRDGRDGRDGKDGKDGKDGK
                     CGCQDKPVTPSNDKPVPPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEV
                     PVKPVPAVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGS
                     GILLSLYVGRRKED"
     gene            complement(3025..3573)
                     /locus_tag="ACL8LA_001107"
     CDS             complement(3025..3573)
                     /locus_tag="ACL8LA_001107"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS1812873.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            3894..4382
                     /locus_tag="ACL8LA_001108"
     CDS             3894..4382
                     /locus_tag="ACL8LA_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGS1812874.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            4379..5566
                     /locus_tag="ACL8LA_001109"
     CDS             4379..5566
                     /locus_tag="ACL8LA_001109"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1812875.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            5559..5924
                     /locus_tag="ACL8LA_001110"
     CDS             5559..5924
                     /locus_tag="ACL8LA_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1812876.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            5940..7286
                     /gene="asnS"
                     /locus_tag="ACL8LA_001111"
     CDS             5940..7286
                     /gene="asnS"
                     /locus_tag="ACL8LA_001111"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGS1812877.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            7439..7729
                     /gene="rpsF"
                     /locus_tag="ACL8LA_001112"
     CDS             7439..7729
                     /gene="rpsF"
                     /locus_tag="ACL8LA_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGS1812878.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            7741..8211
                     /gene="ssbA"
                     /locus_tag="ACL8LA_001113"
     CDS             7741..8211
                     /gene="ssbA"
                     /locus_tag="ACL8LA_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGS1812879.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            8243..8482
                     /gene="rpsR"
                     /locus_tag="ACL8LA_001114"
     CDS             8243..8482
                     /gene="rpsR"
                     /locus_tag="ACL8LA_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGS1812880.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            8777..10177
                     /locus_tag="ACL8LA_001115"
     CDS             8777..10177
                     /locus_tag="ACL8LA_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGS1812881.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            10179..10538
                     /locus_tag="ACL8LA_001116"
     CDS             10179..10538
                     /locus_tag="ACL8LA_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGS1812882.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            10592..11341
                     /locus_tag="ACL8LA_001117"
     CDS             10592..11341
                     /locus_tag="ACL8LA_001117"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGS1812883.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            11331..11630
                     /locus_tag="ACL8LA_001118"
     CDS             11331..11630
                     /locus_tag="ACL8LA_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGS1812884.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            11701..12636
                     /locus_tag="ACL8LA_001119"
     CDS             11701..12636
                     /locus_tag="ACL8LA_001119"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGS1812885.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            12722..13234
                     /locus_tag="ACL8LA_001120"
     CDS             12722..13234
                     /locus_tag="ACL8LA_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGS1812886.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            13218..13867
                     /locus_tag="ACL8LA_001121"
                     /pseudo
     CDS             13218..13867
                     /locus_tag="ACL8LA_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            13968..14165
                     /locus_tag="ACL8LA_001122"
     CDS             13968..14165
                     /locus_tag="ACL8LA_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS1812887.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(14208..15653)
                     /locus_tag="ACL8LA_001123"
     CDS             complement(14208..15653)
                     /locus_tag="ACL8LA_001123"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGS1812888.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVKDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            15759..17381
                     /locus_tag="ACL8LA_001124"
     CDS             15759..17381
                     /locus_tag="ACL8LA_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS1812889.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLCSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            17585..19543
                     /locus_tag="ACL8LA_001125"
     CDS             17585..19543
                     /locus_tag="ACL8LA_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1812890.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSVQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            19715..19948
                     /locus_tag="ACL8LA_001126"
     CDS             19715..19948
                     /locus_tag="ACL8LA_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HGS1812891.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            19966..20487
                     /locus_tag="ACL8LA_001127"
                     /pseudo
     CDS             19966..20487
                     /locus_tag="ACL8LA_001127"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            20674..21396
                     /locus_tag="ACL8LA_001128"
     CDS             20674..21396
                     /locus_tag="ACL8LA_001128"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812892.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            21554..22648
                     /locus_tag="ACL8LA_001129"
     CDS             21554..22648
                     /locus_tag="ACL8LA_001129"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS1812893.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            22656..23822
                     /locus_tag="ACL8LA_001130"
     CDS             22656..23822
                     /locus_tag="ACL8LA_001130"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGS1812894.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            23915..27013
                     /locus_tag="ACL8LA_001131"
     CDS             23915..27013
                     /locus_tag="ACL8LA_001131"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1812895.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            27061..27678
                     /locus_tag="ACL8LA_001132"
     CDS             27061..27678
                     /locus_tag="ACL8LA_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS1812896.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            27689..29023
                     /gene="murC"
                     /locus_tag="ACL8LA_001133"
     CDS             27689..29023
                     /gene="murC"
                     /locus_tag="ACL8LA_001133"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGS1812897.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            29033..29497
                     /locus_tag="ACL8LA_001134"
     CDS             29033..29497
                     /locus_tag="ACL8LA_001134"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812898.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            29494..29988
                     /locus_tag="ACL8LA_001135"
     CDS             29494..29988
                     /locus_tag="ACL8LA_001135"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1812899.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            30069..31724
                     /gene="mltG"
                     /locus_tag="ACL8LA_001136"
     CDS             30069..31724
                     /gene="mltG"
                     /locus_tag="ACL8LA_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGS1812900.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            31795..32277
                     /gene="greA"
                     /locus_tag="ACL8LA_001137"
     CDS             31795..32277
                     /gene="greA"
                     /locus_tag="ACL8LA_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGS1812901.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT         9950 a         6052 c         7275 g         9160 t
ORIGIN      
        1 aaatcaaggt aaagacggtg ataaatacgt caatacagaa acaggcgacg tcttcgttaa
       61 gaataacggc aactgggata aagagggcaa catcaaggga cctaaagggg acaaaggtga
      121 acgcggagaa gatggtaaga ctccagaagt aactgtaact ccaggtaaag atggccatag
      181 tactgacatt acattcactg ttccaggtaa agatccagtt acagtaaatg ttaaggacgg
      241 agaaaatggt ctgaacggta aaactccaaa agttgattta cttcgtgtcc aaggacaaaa
      301 cggaaatcca cctcatacaa ttgtgacatt ctatacagat gaaaacaatg atggcaaata
      361 tacaccagga actgatgaac ttctaggttc agaaatgatt aaagatggtg ctaaaggtgc
      421 ggacggacga gatggtaaat cattgcttac tgtcaaggat ggtaaagaaa ctaaagttta
      481 ccaagaagat ccagctaacc caggacaacc attaaatcca gaaaaaccac ttgcggtaat
      541 tagagatgga gtagatggaa aatcacctac agttacagct gttcgtaaag atgaagcagg
      601 gcataaaggt gtagaaatca ctgttgataa ccatgatggt tcacaaccaa ctacagtctt
      661 tgttcaagat ggtgctaagg gagaaactgg tgcaaccggt caagatggac aaactcctac
      721 aatcactact caacgtggac aagatggcca aagcactgtt gtaactatca caacatcagg
      781 taaagatcca gtaaccttca ctgtaaaaga tggtaagaat ggtaaagatg gccgtgcacc
      841 gaaaatcaaa gtagaagata ttacttcacc ttcaagaatt agacgcgata cagatgctgc
      901 tgcaactcca acgcgtaacg gtatccgtgt tacagtttat gatgatgtta atgacaatgg
      961 ggtatacgac gaaggtgtcg ataaagtatt aaatagtaaa gatatttata acggtataga
     1021 tggacgtgat ggttcagctc caactattac tacaaaagat aatggagatg gaactcacac
     1081 tatcacagtt caaaacccag atggttctga atcaacaaca gttgttaaag atggtaaaga
     1141 cggtaaaact gcgaatatca ctacaacaga aaacccagat ggaagccaca caattacggt
     1201 gacaaatcca gatggttcaa ctaaagaaac tgttgttaaa aacggtaaag acggtaagac
     1261 tcctaaagtt gaagtaacgg ataacaacga tggaactcat actgttaaag ttacagatgg
     1321 agacggcaat gttaccaacg ctatcatcaa agatggtaaa gacggtaaag ctgcaacagc
     1381 aacaactact gaaaatccag atggaagcca cacagtaaca atcactaacc cagacggaac
     1441 taagaatgag tttgttgtta agaatggacg tgacggtgtt gacggacgta ctccaaccgc
     1501 atctgttcgt gataatggag acggaagtca tacaatcgtt attacaaatc cagaaggtgt
     1561 gacaactgaa accacagttc gtgatggtaa atcaccaaaa gtgactataa ctgatgaaca
     1621 aaatggaact cataagatct ctgttctaaa tggtgacgga acaactactg aaacaatcat
     1681 taaagatggt aaatcaccag tagcaacagt tagagataac caagatggta cttacactat
     1741 tcgtgtggaa aacggtaatg gtactgtttc tgaaaccaca gttcgtgacg gtaaatcacc
     1801 aactgctaag gttgtggata atggagatgg aactcacact atcacagttg tgaactcaga
     1861 cggaacaact acaacaacta cagttcgtga tggtagagaa ccaaaacttg aagttattga
     1921 taacaacgat ggttcacaca ctattaaagt gacaggtgct gatggtaaag gaacgacaac
     1981 tacaatcttt gatggtaaat caccaaaagc gaacatcgtt gataacggag atggaactca
     2041 tacattaaca atcgtagatt ctgatggtcg tgaatacaaa tctattatca aagatggtaa
     2101 agacggcaaa gatagcgttt caccaactgt aactgttaaa aataataacg atggaactca
     2161 cgttgttaca atcactaatc cagatggaag taagacagaa atggtgatta aagacggtaa
     2221 agatggtaaa tcaccaaaag tttctgttga agataatggt gatggtagtc atacaatcac
     2281 aatcatcaat tctgatggaa ctgtgacaaa aacagttatt aaagatggca aagatggtag
     2341 agatggacgt gatggtcgag acggcaaaga cggtaaagac ggtaaagatg gaaaatgtgg
     2401 atgccaagac aaaccagtaa caccatcaaa tgacaaacca gttcctccaa caccaaatgt
     2461 gccgacacca gaagtaccgg ttaaacctgt gccagcgcaa ccaacaccaa atgtaccgac
     2521 accagaagtg ccagtacaac caactccagc tgttccaaca ccagaagtac cggttaaacc
     2581 agtaccagcg gttccagaac aaccagtagt accaacaccg gctcaaccag caactccagt
     2641 aaatgctaat ccagtagcac caactacagg taaagaaaac cgtggggaca aattacctga
     2701 aactggaagc caatctgatt atatctctgt tcttttaggt agcggtattc tattgagcct
     2761 atatgtagga cgaagaaaag aagattaatc agccaaaagc agtcactaat ttctcgaaag
     2821 aagatgataa gtgatagatc tcgaatgaaa gaatattctt agaatgattt tcctatgatg
     2881 gaaaagactg ctaaaaacct tgagtggtaa cgctcaaggt ttttttggat ggctgatgtt
     2941 tgtctgaaaa tcttgattga gaaataaaaa gccaccctat taggtggcta taattgattt
     3001 agactttcta aaattttcta ttttttactg aatcgcttcg accaagtctt gagcttgttt
     3061 ttcaagtgag tttaggactg tttcttcaag aaccaatttt ccgtctgccc aggcagagtc
     3121 attaacacgt gcagcagtga aatcaccaac gccttgtgta cggataaatg gcaagaggtc
     3181 tttgtagata gcgaaaagtt gatcgtgccc tgcattggct acagatgata ctgtgacaaa
     3241 cttgtcttga agggcagaaa cgccacgtgt atcagacaag tcaagggcac gagatagcca
     3301 gtcaagcaag tttttcactg taccagggat agagaagttg tagactggag agaaaatcca
     3361 gatagcatcc gcaacgagaa ctgcttcacg agcagcagct acagctggat gtgttggaac
     3421 ttccaaatct tggctgaaga gaggaagggc tgaataatca aggtagctaa cttccgcttt
     3481 accagcaagt gctttctcag cttcgagcgc catttggtgg ttgaaagaac cttgacgtag
     3541 tgatccgacg ataaataata cttttttaga catgatgtgt ctcctttttc ttaaaatttc
     3601 gagaactctc tcgttgttat aaaatatatt acaacaaaac aaaagaactt gcaagaaatt
     3661 tgctcagagt ttatttgaaa attgtaaaat ttcggaaact ctaatcattt gtgactgaaa
     3721 aagtaagaac tgtttcactt tcaaaatcgg ttttgtttga agggagaaat agaattggtt
     3781 gctcaaagca cagctttgag gttgcagata aagctgacgt ggtttgaaga gattttcgaa
     3841 gagtattctt atattcactt gtaataaagc ttaatagatg gtaaaataga cgagtgaaaa
     3901 aaagacaaaa caaagcaaaa aataatctac tgtggcagta tggtctaggg atgacgattt
     3961 tgtttgtggt tatcagtggt tcctttctgt atatggtttc tcttagcatg aaaccctatc
     4021 aaacagctaa aagtgaagga gaaaaattag ctcagcagta tgcaggatta gagcaggtcg
     4081 atcaggttga tttatacaat ggcttggaat cttattacag cgttcttggt cgtaataaac
     4141 agcaagaagc gcttgctgtc ctgattggta aagatgacca taagatttac gtttatcagc
     4201 taaatcaggg tgtttcacaa gaaaaagcag aaacggtttc taaggaaaag ggagctggcg
     4261 agattgacaa gataaccttt ggccgttatc aagataagcc aatctgggaa gttaagtcag
     4321 gatctgattt ttatctagta gattttgaaa caggagcatt ggtcaacaag gagggcctat
     4381 gaaactatcc aaccgtgttt tagaaatgga agaaagtgtg actttggctg ctggagcccg
     4441 tgccaaagca ctgaaggctg agggtagaga tattttgtct ctaaccttgg gtgagccaga
     4501 tttcactact cccaaaaata tccaagatgc cgccattgca tcgattcgag atgggcgtgc
     4561 ttctttttat acagtaacct caggtctgcc agagcttaag gcggcggtca atagctactt
     4621 tgagcgcttt tacggctatt ctgtagcgtc aaatcaagtg acagtcgctg cgggagccaa
     4681 atattctctc tataccttct ttatggctgt ggtcaatcca ggtgatgaag tcatcatccc
     4741 aaccccatac tgggttagct atggagatca ggtcaagatg gcagaaggcg ttcccgtctt
     4801 tgtttctact aaggaagaca atcactttaa ggtgaccgta gatcagttag aagcagctcg
     4861 cactgacaag accaaggttt tggtgctgaa ttcgccatct aatcccacag gtatgattta
     4921 cacccgtgag gaactcttgg caattggaaa ctgggctgta gaaaatgata ttctcatcct
     4981 agcagatgat atctatggcc gcttggttta taatggtcat gagttcacac cgatttctag
     5041 cctatcggaa gcgattcgca agcaaacagt ggtcatcaat ggtgtgtcta aaacctatgc
     5101 catgactggt tggcggattg gttatgccgt tggagaagca gatattatcg ctgccatgtc
     5161 caagattgca ggtcaaacaa cttcgaatcc gtcagcagta gcccaatatg cagcagttga
     5221 ggctctatca ggtgagcaag atactgtaga aagcatgcgt caggcctttg aggaacgtct
     5281 taataccatc tatcccctcc ttgcagaggt accaggattt gaagtggtca aaccgcaagg
     5341 ggccttctat ctcttcccaa atgtcaaaaa ggccatggag atgaaaggct acacggatgt
     5401 gacagacttt acaactgcta tcttagaaga agccgaagtg gccttggtaa caggagctgg
     5461 atttggagca ccagaaaatg tccgcctcag ctatgcgaca gacctagaca cgcttaaaga
     5521 agcagtcgaa cgcttgaaag catttatggg tagtgagaat gattgatcat tttgagatta
     5581 aggtaaagga tttacaaatt tcagaaggat tttataggag ttttctcgct cctttggact
     5641 ataaattggc ttttaagact agttctctaa ttagttttct atccccgaac agccctcatc
     5701 ctggtggtga tttttggctg actcagggaa cacaagatcc tgttcatttt gcttttttag
     5761 cagaaaataa ggaagaagtt caggcttgtt atgaggctgg cttagaggca ggtgggcgag
     5821 acaatggggc tcctggttat cgaagtgagc atccgattta ctatgctgct tttatgattg
     5881 acctggatgg gaacaatata gaggtggttt gccataaaga ataaaaaaga aaaggaaaaa
     5941 taatgacaaa acgtgtaacg attattgacg taaaagacta tgttggtcag gaagtgacga
     6001 ttggcgcttg ggttgccaac aaatcaggaa aaggaaaaat cgctttctta caattgcgtg
     6061 atggaacagc cttctttcaa ggtgtggctt ttaaaccaaa ctttgtcgaa aaatttggtg
     6121 aagaagtggg acttgagaag tttgatgtta tcaaacgctt gagccaagaa acgtctgttt
     6181 atgtgacagg tattgtcaaa gaggacgaac gttctaaatt tggctatgag ttggacatca
     6241 cagacatcga agtgatcggt gaatctcaag actacccaat cacaccaaaa gaacacggaa
     6301 cagacttttt gatggataac cgtcacttgt ggctacgctc tcgtaagcaa gtagctgtgt
     6361 tgcaaatccg taacgctatt atctatgcaa cttatgagtt ctttgacaag aacggtttta
     6421 tgaagtttga cagcccaatt ctttcaggaa atgcggcaga agattctaca gaactctttg
     6481 aaactgacta cttcggaacg ccagcctact tgagccaatc aggtcagctt tacctagaag
     6541 caggggctat ggctcttggt cgtgtctttg actttggtcc agttttccgt gctgaaaaat
     6601 caaaaacacg ccgtcacttg actgagttct ggatgatgga tgctgagtac tcatacttga
     6661 cacatgatga gtcgcttgac ttgcaagaag cttatgtgaa agctcttcta caaggtgttc
     6721 ttgaccgcgc gcctcaagcc ttggaaacct tggaacgtga tacagaactc ttgaaacgct
     6781 acattgcaga gccattcaaa cgtatcactt acgatcaagc cattgacctc ttgcaagagc
     6841 atgaaaatga tgaagatgct gactacgagc atcttgagca tggtgatgac tttgggtcac
     6901 cacacgaaac ttggatttca aaccactttg gtgtgccaac atttgtcatg aactatccag
     6961 cagccatcaa ggccttctac atgaaaccag ttcctggaaa tccagagcgc gtgctttgtg
     7021 cagacttgct tgctccagaa ggctatggag aaattatcgg tgggtctatg cgtgaggaag
     7081 attacgatgc ccttgtcgct aagatggatg aacttggcat ggatcgtaca gaatatgaat
     7141 tctaccttga ccttcgtaaa tacggtacag ttccacacgg aggatttggt atcggtatcg
     7201 aacgtatggt aaccttcgca gcaggaacaa aacatatccg tgaagctatt ccattcccac
     7261 gtatgttgca ccgtatcaaa ccataagaac aggaaaacgc ccatgtggcg ttttttcgaa
     7321 ttctagtgtt tttacttgcg taaaaaaatt ttttctagta taatagttta ttgtgagcga
     7381 acctcactta ccccttgcaa agtcttgggg tcattagacc aaaaggagga acatatcaat
     7441 ggctaaatac gaaattcttt atatcattcg tccaaacatt gaagaagaag ctaaaaacgc
     7501 tttggtagca cgttttgact ctattttgac tgacaacggt gcaactgttg ttgaatcaaa
     7561 aacttgggaa aaacgtcgtc ttgcatacga aatccaagat ttccgtgaag gactttacca
     7621 catcgttaac gttgaagcaa atgacgatgc agctcttaaa gagtttgacc gtctttcaaa
     7681 aatcaacgct gacattcttc gtcacatgat cgtcaaaatt gacgcgtaag aaggtaaact
     7741 atgattaaca atgttgtact tgtagggcgt atgacacgtg acgctgagtt gcgttatacc
     7801 ccatcaaatg tagcagttgc gacttttact cttgcagtaa accgtacatt taagagtcaa
     7861 aatggtgaac gtgaggctga ttttatcaat gtcgttatgt ggcgccaaca ggctgaaaat
     7921 cttgctaact gggctaaaaa aggctcactt atcggggtga caggtcgtat ccagactcgt
     7981 agttacgata accagcaagg acaacgtgtc tacgtgacag aggtcgtggc tgagaatttc
     8041 caaatgttgg aaagccgtag tgtgcgtgag ggccacacag gtggagctta ctctgcacca
     8101 actgcaaact attcagcacc tacaaattca gtaccagact tttcacgtaa tgaaaatcca
     8161 tttggagcaa caaacccatt ggatatttca gatgatgatt taccattcta atggacaact
     8221 aaacttataa aggagaaaaa acatggctca acaacgtcgt ggcggattca aacgccgtaa
     8281 aaaagttgat tacatcgcag caaacaaaat tgaatatgtt gattacaaag atactgagct
     8341 tcttagccgt ttcgtttcag aacgtgggaa aatccttcct cgtcgtgtaa caggaacttc
     8401 agctaaaaac caacgtaaag taacaacagc tatcaaacgc gctcgcgtaa tggctttgat
     8461 gcctttcgta aacgaagatt aaagaaaaga cccggttggg tctctttttc atgagcttga
     8521 aaacaagaaa gcgaattaag accccaacgg gtcttttttc acaagctttg aaatgagaga
     8581 gcgagttagg ctcagtggct ccctttttac gagtttgaaa acaagagaac acattaaaag
     8641 cacgatggag ctttttcata aatatgtctt ttttctcatc caagaaatta aaataaaaat
     8701 gataaacttt ttgaaaatag gggatttgcc tactttatga tataatggaa agagttagat
     8761 tgaaagaggt agtgagatgg acgcgaaatt aagatacaag gcaaagaaga tcaagattgt
     8821 cttttttgat attgatgata cattgcggaa ttcaaagaca gggtttattc caactacaat
     8881 tcccactgtt tttaaacagt tgcgtgaaaa aggaatttta acaggaatcg cctctggacg
     8941 tggcattttt ggtgttgttc cagagattcg tgatctcaag cctgactttt ttgtaacttt
     9001 gaatggagct tatatcgaag ataaaaaagg tcaggtcatt tatcagcatc agattgaaaa
     9061 gtcagatgtt gaggagtata tctcttgggc taagcaagaa ggaattgagt atggcttggt
     9121 tgggagtcat gatgccaagt tgtcgactcg caccgatatg atgagtgaag ctatcaatcc
     9181 aatttatccc gacttagatg tagatcccga tttccatgaa aaagaagata tctatcagat
     9241 gtggactttt gaagataagg gagatgactt gcacttgcct gacagtctct cagacaaact
     9301 tcgcatggtt cgttggcatc aacattcgtc tgatattgtg ccgatttcag gctccaaagc
     9361 gacgggggtg gaaaaggttg tggaacacct tggcttgaaa ccagagaagg tcatggtttt
     9421 tggagatggt ctcaacgact tggaactctt tgattatgct ggaatcagcg ttgccatggg
     9481 aatttctcat gataaaatca aagaaaaagc agattatatt acaaaaacat tagaagaaga
     9541 tggcattttt gatgccttag aagtatttgg tatggtagaa aaagaattgc attttccaca
     9601 agtagacatt gaaacagtag aaggtcctct tgcgactatt aagaccaatc acggagactt
     9661 acgtatcaag cttttccctg aacatgctcc taaaacagtg gctaactttg tatctctttc
     9721 aaaagatggc tactatgatg gtgtcatttt ccaccgtatt atcaaggact ttatgatcca
     9781 aggtggagac ccaactggaa ctggtatggg tggcgagtca atctacggcg aatcttttga
     9841 ggatgaattc tcagaagagc tttacaatat ccgtggtgct ctttccatgg caaatgctgg
     9901 tccaaatacc aacggcagcc agttctttat cgtgcaaaac caacacctac cttattcgaa
     9961 gaaagaaatt actcgtggtg gttggccaga accgattgca gaaatctatg ccaatcaagg
    10021 tgggacacct cacctagacc gccgtcacac ggtttttggt cagttagctg atgaagcatc
    10081 ttacgctgtc ttggatgcca ttgctgctgt tgagacagga gctatggaca agccagttga
    10141 agatgttgta attgaaacta ttgaaatcga ggactaagat gaaaatcggt gataagctaa
    10201 aggggcgtat tacagggatt cagccctacg gtgcctttgt tgagttagag acgggtgata
    10261 cggggctgat tcacatttca gaaattcgga caggatttat tgaaaatatt catgaaacct
    10321 tgaaagtcga tgaagaggtt caagttcagg tagtggattt agatgaattt acggggaaag
    10381 ccagtctttc tatccgcact ttggaggaag aaaagtacca gtttcctaga cggagacgct
    10441 tttcaagcga ccgctttaac tacggttttg ctccctttcg acgaatgcta ccaatctgga
    10501 ctggagaagc cctgcaccat ttaaaaaaga agaagtagtt tagagtttta tattttttgt
    10561 aatgttaata tgaatttgct ataataggac catggaagaa gaacaattat taaaatcagg
    10621 agagcgcatt aaccagctct tttcgacaga tatcaaaatc attcaaaata gagaggtttt
    10681 tagctattcg gtggatagtg ttctcttatc acgatttcca cgttttccta agaaggggtt
    10741 gattgtggat ttctgtgctg ggaatggagc agtggggctt tttgctagca ctcgtactca
    10801 agcacagata ttgtctgttg agattcagga gcgtttggcg gatatggctg aacgctctgt
    10861 ccgtttgaat ggattagagg agcaaatgca ggtcatctgc gatgatttga aaaatatgcc
    10921 tgctcacatt caggggagta aggtggatat gattttgtgt aatccaccct atttcaaggt
    10981 gaatccttat tctaatctga acgagagtga acattatctc ttggctcgac acgaaatcac
    11041 gactaatttg gaagaaatct gtcgtagtgc ccagagtatt ctcaagtcta atgggcgttt
    11101 ggccatggtt catcgtcctg atcgacttct ggatattttg gatacgttaa aacgacataa
    11161 tctagcccct aagcgcctgc agtttgttta tccaaaaaga gaaaaggaag ccaatatgct
    11221 tttgattgag gcgatcaagg atggctcaac aagtggcttt aaggtcttac ctcctttgat
    11281 tgtccacaat gatgatggct cttatacgcc cgaaatcgaa gagatttatt atggatcata
    11341 aggcttatat gtacgttctg gagtgtcgtg atggatccta ctatataggc tatacgactg
    11401 atatgagaag acgcctcgct atccacaata gtgggaaggg agccaaatat acacgagcac
    11461 gcttgccagt caaacttatc tatgctcaag gttttgccag taaggaagaa gccatgtcgg
    11521 ctgaagctct tctcaagcgt aagaagaggc cacagaagga agaattttta tctgaaaatc
    11581 aagatagaaa tttactcagt tattttgaag aatcgtgggg agtcctttga ctcctcttac
    11641 ctttgtcaaa aagcgaaaaa aatgctacaa taaagaaaat aaacaaaatg aggtattatc
    11701 atgtccaaga ttctagtatt tggtcaccaa aatccagact cagatgccat cggatcatct
    11761 gtagcttttg cctaccttgc aaaagaagct tacggtttgg atacggaagc tgttgccctt
    11821 ggaactccaa atgaagaaac agcctttgtc ttgaactatt ttggtgtgga agcaccacgt
    11881 gttatcactt ctgccaaagc agagggggca gagcaagtta tcttgactga ccacaatgaa
    11941 ttccaacaat ctgtatcaga tatcgctgaa gtagaagttt acggtgttgt agaccaccac
    12001 cgtgtggcta actttgaaac tgcaagccca ctttacatgc gtttggagcc agttggatca
    12061 gcgtcttcaa tcgtttaccg tatgttcaaa gaacatggtg tagctgtgcc taaagagatt
    12121 gcaggtttga tgctttcagg tttgatttca gatacccttc ttttgaaatc accaacaaca
    12181 cacccaacag ataaaatcat tgctcctgaa ttggctgaat tggctggtgt gaacttggaa
    12241 gaatatggtt tggcaatgtt gaaagctggt accaacttgg ctagcaaatc tgctgaagaa
    12301 ttgattgata tcgatgctaa gacttttgaa ctcaacggaa ataatgtccg tgttgcccaa
    12361 gtgaacacag ttgacatcgc tgaagttttg gaacgccaag cagaaattga agctgcaatg
    12421 caagctgcca acgaatcaaa cggctactct gactttgtct tgatgattac agatatcgtc
    12481 aactcaaact cagaaatctt ggctcttggt gccaatatgg acaaggtcga agcggctttc
    12541 aacttcaaac ttgaaaacaa tcatgccttc cttgctggtg ccgtttcacg taagaaacaa
    12601 gtggtacctc aattgactga aagctttaat gcgtaagatt ttgggtgtca gctcaaaatc
    12661 ggaaagtcta gtttgcctta tatcgaaagg agtttcggct ccttttttct aggagtgaag
    12721 tatgttagaa aatggcgatt tgatttttgt gagagatggg tcagacatgg gacaggccat
    12781 ccagacttcc acaggtaact atagccatgt tgccatttat ttggatggga tgatttatca
    12841 tgctagtgga caggctggtg ttgtctgtca agaaccggca gacttctttg agtccaatca
    12901 tttatacgac ctctatgttt acccagaaat ggatatccag tcggtgaagg aaagagcttg
    12961 caaacatctt ggagcaccct acaatgcttc tttctatcca gatgcacctg gtttctactg
    13021 ctcccagtat atagcagaaa tcctacctat ttttgaaact attcctatga aatttggaga
    13081 tggggagcag gagattagtg atttttggag ggagtattac atagaactag gtctgcctgt
    13141 tcctctgaac caagctggta ccaatcctag tcagttggca gcatcgcctc tgttacaatg
    13201 taaagaaagg aatcttcatg attcagattt ttaatccatc tcgtttgacg agacagccat
    13261 ttttggagaa ttgatccgct atctggacca gtatgaggat gtgattctac gggaaattaa
    13321 ggctcaattt ccagatgttg cagttgataa actcatggaa gagtatataa aggcaggctt
    13381 gattctacgt gaaaataagc gctattacct caattttcct acgcttgaat cacttgatag
    13441 tcttgaactg gatcaagaga tttttgtcag agaagctagt ccggtctatc aagccttgtt
    13501 ggagcagagt tttgagacgg aattgcgcaa tcaaatcaat gcagctattt tagttgaaaa
    13561 gacggacttt gcgcgcatta aaatgactct gtccaattat ttttacaagg tcaaacagca
    13621 gtatcctttg acagaaaaac agcaggagct ctatgacatt ttaggagatg ttaatcctga
    13681 gtatgcactc aagtatatga cggctttttt gttgaaattt ctcaaaaaag accagcttat
    13741 gcagaaatgc cgtgatatct ttgtggacag tttggttgtc ttaggctata ttgtgcaaaa
    13801 tgaagatgga aagtatgagt tggctatcga ttttgataag gagagattaa ctttctactt
    13861 agcgtgattt cttgtttctg agtacattgt ttgactttcc ttagtattcg gtataaacta
    13921 tatgtaaccg gtaacacata tcggaataaa ctaaaggaga caatcatatg tcacttgaaa
    13981 acaaattgga acaagcaaca ggcgctgtca aagaaggttt tggtaaagtt actggagaca
    14041 gcaagacaga acttgaagga gctgttgaaa aaacagttgc taaggcaaaa gacgttgtag
    14101 aagacgcaaa aggtgctgta gaaggtgccg ttgaaggttt gaaaaacgtt tttactaaag
    14161 aataggaaaa aatcaagggt ttcattttcc cttgattttt tctattctta taaataattt
    14221 tctgcgacgg ctgtatctcc tgggtaggat tctttcttgc cctggatgat ttggtaacaa
    14281 tcggctccct tacccgcaat aataactgca tctaattcgt gatttgtgat agccattgcc
    14341 gccttgatgg cttcttggcg atccgcaatc ttctcaacag gatgattgat gtagctacta
    14401 atttcatctg caatggccat tgggtcttca tagttagggt catcagcagt cagaaagact
    14461 tgaatctcag ggtgttgatt gaggaggagg ccaaagtcct tacgacgact ttctcccttg
    14521 tttcctgttg atcccagaac cagagcaatc tttccggttt gatgagtttc aaccacattg
    14581 atgagttttt tcagactatc cccattgtgg gcatagtcga tgaagacctt ggctccattt
    14641 ttctgagtga ggacttccat acgaccagga acgcgggttg cagcgatgcc ttttttgatg
    14701 tcctcaagac ttgctccgag acggagacaa gcaagtccag cagcaactgc attttcttgg
    14761 ttgaagttgc caatgagttg gatatcataa tctccagcga gtttacccgt agctgaaaag
    14821 ctaaaggctt tggaattctc gatttggtta tcaaattggc taccatagaa atcatggtct
    14881 tgatctttaa cctgttcttt caagactgag aagtggtcca tgtcactgtt aatgatgact
    14941 gctcggctat tttccatcaa gagacgcttg tggtagaaat agtcttcaaa gctagggtgt
    15001 tcaatcgggc cgatatggtc tgggctgata ttgaggaaaa ctcccacatc aaaggttaga
    15061 ccatagacac gtctgaccag ataggattga ctggagactt ccatgatgag gtgggtacgg
    15121 tcattttgca cagcctgatt catcatgtca aagaggtcaa tactctcagg ggttgtcaac
    15181 gctgacttaa agaaagtctc gccatcaaga gttgtgttca tggtcgacaa catagcaggt
    15241 cgatgccctt gagataagat gttataggcg aaataggctg ctgttgtctt acccttagta
    15301 ccagtaaagg caaggagttt gagtttttcc tgtggattac catagaactc catggcaatc
    15361 aaactcatgg ctttctttat atcgttcaca atgatgacag ggataccgac ttcgtagtcc
    15421 ttttcagcta cataccaagc taagccttgt gttatagcag aaagaaggta ttctttttta
    15481 aaggcagcgc cttttgcaaa aaaaagagtg tcttctgtta cttttcggct gtcgtagctg
    15541 atgctatcaa aaacaacttt gctgtagttg tagtggtaat gaccttggtc aataatttcg
    15601 cgaaaaaggc catctttctt taaaatatct aatacagttt caatttttat catactttct
    15661 attgtaaacc gaaagtcgca aatttacaag taacaaggaa aagtttataa tggaagataa
    15721 ggagtttttc ctagttatca aaattgaatg aggaatctat gtcgcacgaa aacaatcacc
    15781 agcaggccca gatgttacgg gggactgctt ggctaacggc tagtaacttt atcagtcgcc
    15841 tactcggggc tgtttacatt atcccttggt acatctggat gggggcttat gcagctaagg
    15901 caaatggtct ctttaccatg ggttacaata tctatgcttg gttcttgttg gtttcaacag
    15961 cggggattcc agttgcggtg gccaagcaag ttgccaagta taataccatg cgagaagaag
    16021 agcatagctt tgccctgatt cggagcttct taggctttat gacaggacta ggcctggttt
    16081 ttgctttagt cttgtatgtc tttgctcctt ggctagcaga cttgtctggc gtgggcaaag
    16141 acttgatccc aatcatgcaa agcttggctt ggggagtctt gattttcccg tctatgagtg
    16201 ttatccgagg atttttccaa gggatgaata acctcaaacc ctatgccatg agccaaattg
    16261 ctgagcaggt cattcgtgtt atctggatgc tcctagcaac ctttatcatt atgaagctcg
    16321 gttcaggaga ttatctagca gccgttaccc aatcaacctt tgctgccttt gtcggtatgg
    16381 tagccagttt tgcagtcttg atttatttcc ttgcccaaga aggttcactc aaaagagtct
    16441 ttgaaacagg agataagatt aacagtaagc gtctcttggt tgataccatt aaggaagcca
    16501 ttccttttat cctgacaggg tctgccatcc agctcttcca gattttggat cagctgacct
    16561 ttatcaatag tatgagctgg tttaccaact acagcaatga ggacttggtt gtcatgtttt
    16621 cttatttctc agccaatcct aataaaatca cgatgatttt gatttctgta ggggtttcga
    16681 ttgggagtgt tggtttgcca cttttgacgg aaaactatgt caagggggac ttgaaagcag
    16741 cttctcgtct ggttcaggac agtctcaccc tactctttat gttcttgcta ccagcaacgg
    16801 ttggagtggt tatggtagga gaacctctat atacagtctt ctatggtaag ccagatagtt
    16861 tggctctggg cttatttgtc tttgcagttt tgcagtctat tattttaggc ttgtacatgg
    16921 tcttgtctcc aatgcttcag gccatgttcc gcaaccgcaa ggccgttctc tattttatct
    16981 atggttctat tgccaagcta gtcttgcaac tacctaccat cgccctcttc cacagttatg
    17041 gtcctttgat ttcaacaacc attgctctca tcattcctaa cgtcttgatg tatcgggata
    17101 tttgtaaagt aactggtgtc aagcgcaagg tgattttgaa gcgaaccatt ttaatcagtt
    17161 tgctgaccct agtcatgttt ctgttaatag gaaccatcca gtggctgtta ggatttttct
    17221 tccaaccaag tggacgtttg tgtagcttct tttatgtagc tcttgtcggt gccatggggg
    17281 gtggacttta tatggttatg agtctgcgta cctatttatt agataaggta ataggaaaag
    17341 cccaagcaga tcgcctgcga gcaaaattta agctttcgta aggtagcatt tataaataaa
    17401 aggaataagt tgaacaatct tttaggagaa cttgaagctt attccttttt tagcgtaaaa
    17461 atagctaaaa ttatatttca aaaatagaaa atgaggcttt tttcgatttt ttggaaaaaa
    17521 tcattgacta aacaaactaa acgttgtata ataaaacaaa tatttaaagc aggaggttcc
    17581 ggaaatgaaa aagtctaaga gcaaatatct aaccttggca ggtcttgtcc tgggtacagg
    17641 agttttattg agcgcgtgtg gaaattctag cacggcgtca aaaacataca actatgttta
    17701 ttcaagtgat ccatctagct tgaactatct agcagaaaac cgcgcagcaa catccgatat
    17761 tgttgcaaat ttggtagacg ggttattaga aaatgaccaa tatgggaata ttattccatc
    17821 attagcagag gattggactg tttctcagga cggtttgacc tatacctaca aacttcgtaa
    17881 ggatgccaag tggtttactt ctgagggaga agaatatgcg cctgtaactg cccaggattt
    17941 tgtgacaggt ttgcaatatg cagctgataa aaaatcagaa gccttgtatc tagtgcagga
    18001 ctctgttgct ggtttggatg actatatcac tggtaaaaca agcgactttt caactgtcgg
    18061 tgtcaaggca cttgatgacc aaacggttca atatactttg gttaaaccag aactttactg
    18121 gaattcaaaa acacttgcaa cgatactttt tcctgttaat gcagatttcc tgaaatcaaa
    18181 aggggatgat tttgggaagg cggatccatc tagtattttg tacaatggac ctttcttgat
    18241 gaaagcactt gtctcaaaat ctgctattga atataagaaa aaccctaatt actgggatgc
    18301 taagaatgtc tttgtagacg atgtgaaatt gacctactat gatggtagcg accaagaatc
    18361 actggaacgt aattttacag ctggtgctta tactacggct cgtctttttc ctaacagctc
    18421 cagctatgaa gggattaaag aaaaatacaa aaacaatatc atctatagtg tgcaaaattc
    18481 aacttcatat ttctttaatt ttaacctaga taggaagtct tacaattata cttctaaaac
    18541 aagtgacatt gaaaagaaat cgacccagga agcagttctc aataaaaact tccgtcaggc
    18601 tatcaatttt gcttttgaca gaacatctta tggggctcag tctgaaggga aagaaggtgc
    18661 aacaaagatt ttgcgtaacc tagtggttcc tccaaacttt gtcagtatca agggaaaaga
    18721 ctttggtgaa gttgtagcct ctaagatggt caactatggt aaggaatggc aaggtatcaa
    18781 ctttgcggat ggtcaagacc cttactacaa tcctgagaaa gccaaggcta agtttgcgga
    18841 agctaagaaa gaactcgaag caaagggtgt tcaattccca atccacttgg ataagactgt
    18901 ggaagtaaca gataaagtag gcatacaagg agttagttct atcaaacaat caattgaatc
    18961 tgttttaggt tctgataatg tagtgattga cattcagcaa ttaacatcag atgagtttga
    19021 cagttcaggc tactttgctc aaacagctgc tcagaaagac tatgatttat atcatggcgg
    19081 ttggggacct gattatcaag acccgtcaac ctatctcgat atttttaata ctaatagtgg
    19141 aggagttcta caaaatcttg gactagagcc tggagaagcc aatgacaagg ctaaggcagt
    19201 tggactggat gtctatactc aaatgttgga agaagctaat aaagagcaag atccagccaa
    19261 acgttatgag aaatatgctg atattcaagc ttggttgatt gatagttctt tagttcttcc
    19321 aagtgtttcg cgtgggggaa caccatcatt gagaagaacc gtaccatttg ctgctgccta
    19381 tggtttaacc ggtacaaaag gggttgaatc atataaatac ctcaaagtac aagataagat
    19441 tgtcacaaca gacgaatatg caaaagccag agaaaaatgg ttgaaagaaa aagaagaatc
    19501 caataaaaaa gcccaagaag aattggcaaa acatgtcaaa taaaatctaa ttgtagataa
    19561 gtttgtgtaa gaagtctttg tgcttaaaaa cgcataatat caggtatata aaatcctaga
    19621 tgttatgcgt tttgttatag gaagatttat tagattttat cctcaaattg agttattccc
    19681 gactttatcc gttgaaatat tataaggagt atttatgaaa ttaaaattat taagagtaga
    19741 tactaaggtg attatgggga gtttcttact tgttctgtct agtctacttg ctttgttgct
    19801 tccccttatc ttaaagggtt taatagatgg gagttctatt gaaaatatag gttccaaagt
    19861 atttcaatcg tttttgattt ttattggtca agccttgttt tcttctattg gttactatct
    19921 gtttagtcaa tcgggtgaaa aaagatagca aaaatcagga aaaaagtgat agaggggttg
    19981 atttatgcag agaaatcctt ctttgataag agccaaagtg gggagttgac ttctgccatt
    20041 gtcaatgaca tgagtgtcat tcgtgagttt ttaattacga ctttcccaaa tattattctg
    20101 agtttagtta tggtacttgg ttccattgta gtcttattta gtcttgattg gaatctttct
    20161 ctacttttat tcatcactct tccttgtatg atgtttatta tcttgcccct ttccaatatc
    20221 agtgaaaagt atagtcgtcg tttacaggag gaaatcggat ttttaacagg gcaattgact
    20281 gaaaagattc aagaacatga attaattaag actaaccaag cagagaaaag tgtacaggat
    20341 gttttggata attgtattga aagggtacaa aacaattcac tgaaatcaga tagggttact
    20401 tcttttgaga ccccgtttgc tctcttattt atctttgcga ctatagttgt gatgctaacc
    20461 tatgggggtt atcgggtcag cgcaggatat atatctgtgg gaaccttggt ttcgtttttg
    20521 atttacctct ttcaattact taatcctatt agtaatatag ctaattttgt aactatttat
    20581 tctagtagca agggatcttc agttgcactg gagaacttgc ttgcagttcc taaagaaaaa
    20641 tttgagggag gaaaatcggt atcaggacaa gggttgaatt ttaaccatgt ctattttggt
    20701 tatgatgaaa atcgacctgt cttaaaggat attacttgtt caattttcaa ggggcaaaaa
    20761 attgcttttg ttgggccatc tggatcagga aaatcaacga ttgtgcgttt gttagagcgg
    20821 ttttataaac cgctttcagg agatattcta atggagcaat caagtatata tgattttaac
    20881 ttaaaagaat ggagaagtaa aatcgcttgg gtttcacaaa ataatgcagt cttatctggc
    20941 agtattcgtg acaatctttg tctcggtttg aatcgcttag taactgatga tgaattgatg
    21001 aaagtgctag acttagtatc actaggtgat gagattcgct ccatgaaaga gggactagat
    21061 actgaagttg gtgaacgcgg acgactcttg tcaggggggc gaagccaaag acttcaaata
    21121 gctagagcct acttaaaaga tgctgaaatt cttatatttg atgaagccac tgctaatctt
    21181 gatgcggatt ctgagtatgc gattatcagt agcctctatt ctgtactaaa ggagaagacg
    21241 gttgtgatta tagcgcatcg cttgtcaacg gtaaaagatg tggattgtat tttcttctta
    21301 gaggagggga aaatcactgg ctcaggaact cataaggaac tactggaaaa tcatgagcgt
    21361 tatgctcgtt ttgtgcagga gcaaatgata gagtgaaggt gtcttttgag attcaccatt
    21421 ttatagtcta ttaaagggag caggaaaaac tcccttttta tatagtttga aactataact
    21481 agctcttgaa aagaagaaaa tgagttgatg aaaataagtg gtacaatagt tactatagat
    21541 ttggaggaat tgtatgagca aggaattaca cattaacaca attttagcac aagcagggat
    21601 caagtctgat gaggcaacag gtgctttggt gacaccactt catttttcaa cgacctatca
    21661 gcatccagag tttggtcgat ctactgggtt tgactatacg cgcactaaaa atccaactcg
    21721 tagtaaggct gaggaagtct tggcggctat tgagtcagca gactatgccc tagcgactag
    21781 ctcagggatg tcagctattg tactggcctt tagcgtcttt ccagtaggaa gtaaggtctt
    21841 ggcagtgcgt gatctttacg gtggttcttt tcgctggttt aaccaagtgg agcaggaagg
    21901 tcatttccat tttacctatg ccaatacaga agaagagttg attgccgagt tagaaaagga
    21961 tgtggatgtt ctctatatcg aaaccccaac caatcccttg atgttggaat ttgatatcga
    22021 aaaactagca aaattggctc atgctaaggg tgccaaagtg gtggtggaca ataccttcta
    22081 tagccctatc taccaacgtc cgattgaaga tggagcagat atcgttctcc attcagcaac
    22141 caagtatcta gcaggccaca atgatgtctt ggctggagtg gttgtgacca atagtttaga
    22201 actatacgag aagctttttt acaatcttaa tacaacaggg gcagtcttgt ctccatttga
    22261 cagctaccag ttgcttcgtg gtctcaagac cttgtctctt cgtatggagc gctcaacagc
    22321 taacgcccaa gaagtggttg cctttttgaa ggattctcca gcagttaagg aagttctcta
    22381 cactggtcgt ggaggcatga tttcctttaa agtagccgat gaaacacgca ttcctcatat
    22441 tttgaacagt ctcaaggtct tctcttttgc ggaaagtttg ggtggagtgg aaagtcttat
    22501 tacttatcca acgactcaaa ctcatgctga tattccagca gaagtacgcc attcttatgg
    22561 tttgacagat gacctcttgc gtttgtctat tgggattgag gatgctagag atttgattgc
    22621 agatttgcgc caagccttag aaggataaga caaagatggg aaaatatgat tttacaagcc
    22681 tgcccaaccg tttagggcac catacctata aatggaaaga aacagaaacg gatagtgaag
    22741 ttctaccagc ttggatagcg gatatggact ttgtagtctt gcctgaaatc caccaagccg
    22801 tgcaaactta cgcagaccaa ctggtttatg gttataccta tgccagtgaa gacttaatta
    22861 aggaagttca aaagtgggaa gctacacaat acggttacaa ctttgacaaa gaggctcttg
    22921 tctttatcga gggtgtggta ccagccatct caacagctat tcaaaccttt acaaaagaag
    22981 gcgaggcggt tttaattaac acgcctgtct acccaccctt tgctcgcagt gtcaagttga
    23041 ataatcgtag attgattact aattccttag tggaaaagga tagtctgttt gagattgact
    23101 ttgaccaact tgaaaaggat ttggtggaag aggaggttaa actctatgtt ctttgcaacc
    23161 ctcacaatcc tggtggacgt gtttgggaaa aagaagtgtt ggagaagatt ggccaactct
    23221 gccaaaaaca cggtgttttg ttagtttcgg atgagattca ccaagatttg accctctttg
    23281 gtcacaaaca ccagtctttc aataccatca atcctgcctt caaaaatttt gctatcgtct
    23341 tgagcagtgc cactaaaaca tttaatattg ctggaacaaa aaattcctat gcagtcattg
    23401 aaaatcctaa gttgagactg gctttccaga aacgcctgtt ggccaataat cagcatgaaa
    23461 tttcaggctt gggttatttg gcgacagaag ctgcctatcg atacggtaaa gattggctag
    23521 aggaactcaa gcaagtcttt gaagaccaca tcaattatgt ggtggatcta tttggaaaag
    23581 agactaaaat caaggtcatg aaaccgcaag gtacctactt gatttggctt gacttttcag
    23641 cttatgacct gactgatgaa acattgcaag agttgttgag aaatgaagcc aaggttatcc
    23701 tcaaccgtgg tttggatttt ggagaggaag gaagtctcca tgcccgcatc aatatagcta
    23761 tgcccaaatc tctgttgcaa gaagtctgtc agcggattgt ggctactttt gccaaatgtt
    23821 aaaaatccag ccttctagga gaaaagtctt cctagaaggc tattttcata ggcgaaaata
    23881 tggtataata aaaagataag gtaaaggtga aaatatggct aaattgattc cggggaaaat
    23941 tcgtatcgaa ggtgttgccc tttatgaaac tggtaaggtt gatatcatca aggaaaagaa
    24001 caatcggctc tacgctcgcg ttgcagaaga agaactgcgc tatagtttag aggatgattt
    24061 gattttttgt gcctgtgatt tttttcaaaa gaggggctac tgtgtgcatt tggcagcgct
    24121 agagcatttt ctgaaaaatg atgagcgtgg tcaggaaatc ttgtggagtc tggaagaagg
    24181 tcatgaagaa aaagaggccg ttgaaaccaa ggtgaccttg ggtggcaagt ttttggatcg
    24241 aattttatct ccaaaatcag aatgcgccta tgagttatca gctgtgggtc aggtggaagc
    24301 aggaaccaat catatcttgt ggactctgag aatcggccaa atcaatagcc aaaaatacta
    24361 tgtcattcgg gatattccac tttttttaag gattgtcgag cagagaaagt cgtatatgat
    24421 tggcaagatt tatgaggagt ctctttcttg ggaagccttt gatgatgcca gccaagaact
    24481 tttgactttc ttgcacggac taacggagga agggctggtt ccagacctct ttttccaaaa
    24541 ccaaggtcgg cacctctttt tccctctgac cttttttgag cagggtgtga atttactgat
    24601 ggccttgccc cattttcaat ttgaccatca agtggatagc taccaaaccc tgctttttca
    24661 ggatatgcat gctgatgcca atctatttgc ctttacagta acagaatatt cggactattt
    24721 tgaaatggaa atcagtgaga gtcctagggt caatgtcttt tatcaaggag cagtactttt
    24781 ccataaaggt caggtttatt ttttaacaga ccagcagatg cgtcttctca aggaaattaa
    24841 agcgttacct gtggatcgac aaggaaagaa atacctgcaa tttgatagca gtgatcgaga
    24901 caaattggcc tcctgtctga ctctttttat ccagatgggc accgtttcag ctccagaacg
    24961 tctacaaatc aaaacttttg cgccctcttt ttactttgac agggaagaag ataatcggat
    25021 tcgtttagaa attcagtttg attatggaaa tagacaggta tctagccgac aagagctaga
    25081 agaattacca ttttcaagtg acgcggactt agaagaaaga attttccaag tctgtttggc
    25141 tgcgggcttt gaagccgatt ttcaatcttg gcgtcaggcc ttaaaagcgg agtctgtcta
    25201 tcattttttt catgatatca ttccagtctt tgaaaaactc gggcatgttg acctatcaga
    25261 taaactagaa gagctttata gcctagcgag tcctcaggtg cagattgcct ccaagggagg
    25321 tctcttggaa atccagtttg attttcaaga tattgcccag gaggaaatcg accaagccat
    25381 acaggccttg gttgccaatc aggattttta tattgattcg tctaatcaag tttacttttt
    25441 cgatgaagaa accaagaaaa ttcgccaaaa tctacaggaa ctggggcaat ttgaattaaa
    25501 agatgggacc ttgcaggctc ggaaatcctt ggcctacagt ttagctcatc tctttgaagg
    25561 gcgagaccgt gtttcttttt cacaagaatt ccagaatctg gcccaggatt tgacgcatcc
    25621 agaggacttt cctttgcaag caactcaggt caaggctgac ttgcgagatt atcaggaaaa
    25681 gggaattggc tggttgcaga tgctccatca ttatggtttt ggtgggattt tggctgatga
    25741 tatgggactt gggaaaaccc ttcagaccat tgcttttttg actagtcaag tgacaaaaga
    25801 aagtcgggtt ttgattctcg ctccgtcggg tttgatttac aactgggcag atgagtttca
    25861 gaaatttgct ccacagttgg atgtggctgt tgttcatggt ttgaaagcaa gtcgtgagga
    25921 gattcttgcc gagagccatc aaatctatgt gactagctat gccaccttcc gtcaggacag
    25981 tgagctttat caggggatgg cctttgactt ccttttctta gatgaagctc aggtcatgaa
    26041 aaatgcccag accaagattg cccagacctt gagacaattt gtggtgccgt cggtctttgc
    26101 cttgtcagga actccgattg aaaaccatct gggtgagttg tggtctattt ttcaaatcgt
    26161 catgcccgga cttttgccaa gcaagaaaga atttatgaaa ttgccagcag agcgagtggc
    26221 tcagtttatc aagcctttcg tgatgcgacg caagaaagaa gaagttctga ctgaattgcc
    26281 agacttgatt gaggtggttt ataaaaatga actggaagac cagcaaaagg ctatttacct
    26341 tgcccagtta caacagatgc gagaccatct ggctcaagtg tcagaacagg aatttcagcg
    26401 aagtcgtgtg gaaattttat ctggtttgat gcgcttgcgt caaatatgtg acactcctgc
    26461 cctgtttatg gaagattatc agggagccag cggcaaacta gatagtctcc gagacctact
    26521 ggtacaggtg gcagacggcg gacaccgtgt cttgattttc tctcagttca agggaatgtt
    26581 ggaaaaaatt gaacaagaac tgccagactt gggcctgaca tcctttaaaa ttacgggttc
    26641 aaccccagcc aaggaaagac aagacatgac caaggccttt aaccaaggag aaagagatgc
    26701 ctttctgatt tccctcaagg ctggtggggt cggtctgaac ctgacaggtg cagatacagt
    26761 gatcttggtt gacctttggt ggaatcctgc ggtggaagcg caagccattg gccgtgccca
    26821 tcggatgggt caggaagaaa cggttgaggt ctatcgcttg gtgaccaagg gaaccattga
    26881 agaaaaaatt caggaactcc aagaacaaaa gaaacatctg gtgtcacaag tattggacgg
    26941 cacagagtca cgtggtagtc tgaccctagc agaaattaga gaaattttgg gaatttctga
    27001 agccagcact tgaaaaatca agacaatacg ctataatgaa gtgttgaaag gaaatagaaa
    27061 atgaaacaag aacgatttcc attggtgtca gatgacgagg tcatgttgac tgaaatgcca
    27121 gtcatgaatc tctatgatga gtctgatctg atcagtaata tcaagggtga gtatcgagat
    27181 aaaaattatt tagaatgggc tcctattgct gaagaaaaac cagtaaaacc gattgaaaag
    27241 caagtaggga aacctaaaaa agctccttta ggggttaaaa aagaaggaaa gagctatgcg
    27301 gaggtggcgc gtgaagaagc gcgtgcagac ttgaaaaaga aacgctctgc taactatcta
    27361 actcaggatt tcagccttgc gagacgtcat tctcagccca gtctagttag acagggcaat
    27421 caaccgacaa ctcctttcca aaaggaaaat cctggtgaat ttgtcaaata tagccaaaaa
    27481 ttgacccagt ctcattatat cttggcggaa gaagttcatt ctatccctac caagaatgaa
    27541 gaagtgtcag cacctgctcc aaagaaaaac aattatgatt ttctaaagaa gagccaaatc
    27601 tacaataaaa aaagtaaaca aacagaacaa gaacgtcggg ttgcccaaga gttgaatctg
    27661 accagaatga cagaataggg gagaaaacat gtcaaagaca tatcatttta tcggaattaa
    27721 gggatcaggg atgagtgcct tggccttgat gttgtaccag atggggcaca aggttcaggg
    27781 atcagatgtt gaaaagtact actttaccca acgcggtctt gagcaggcag gaattaccat
    27841 tcttcctttt gatgaaaaga atctagacgg tgatatggaa attatcgctg gaaatgcctt
    27901 tcgtccagat aacaacgtcg aaattgccta tgcggaccaa aatggtatca gctacaaacg
    27961 ttaccatgag tttctaggta gctttatgcg tgactttgtt agcatgggag tagcaggagc
    28021 acatggaaaa acttcaacga caggtatgtt gtctcatgtc ttgtctcaca ttacagatac
    28081 cagcttcttg attggagatg ggacaggtcg tggttcggcc aatgccaaat attttgtctt
    28141 tgaatctgac gaatatgagc gtcacttcat gccttaccac ccagaatact ctattatcac
    28201 caacattgac tttgaccatc cagattattt cacaagtctc gaggatgttt tcaatgcctt
    28261 taacgactat gccaaacaaa tcaccaaggg tctttttgtc tatggtgaag atgctgaatt
    28321 gcgtaagatt acgtctgatg caccaattta ttattatggt tttgaagctg aaggcaatga
    28381 ctttgtagct agtgatcttc ttcgttcaac aactggttca accttcaccg ttcatttccg
    28441 tggacaaaac ttggggcaat tccacattcc aacctttggt cgtcacaata tcatgaatgc
    28501 gacagccgtt attggtcttc tttacacagc aggatttgat ttgaacttgg tgcgtgagca
    28561 cttgaaaaca ttttccggtg ttaaacgtcg tttcactgag aaaattgtca atgatacagt
    28621 gattatcgat gactttgccc atcatccaac agaaattatt gcgaccttgg atgcggctcg
    28681 tcagaaatac ccaagcaagg aaattgtagc agtctttcaa ccgcatacct ttacaagaac
    28741 cattgccttg ttggacgact ttgcccatgc tttaaaccaa gcagatgctg tttatctagc
    28801 gcaaatttat ggctcggctc gtgaagtaga tcatggtgac gttaaggtag aagacctagc
    28861 caacaaaatc aacaaaaaac accaagtgat tactgttgaa aatgtttctc cactcctaga
    28921 ccatgacaat gctgtttacg tctttatggg agcaggagac atccaaacct atgaatactc
    28981 atttgagcgt ctcttgtcta acttgacaag caatgttcaa taggatgttc ccatggaaat
    29041 tccaattaag atcattcagg caagcaagtc tgatttgcct gagatagggg cacttcagac
    29101 ctcgtctttt ccagctgaaa agcagcaact ttcccatatt ttagaagaaa gtatccgtaa
    29161 gtgtgcggat acctttcttc tagctaggga tgaaaatcaa cttttaggct atattttatc
    29221 aagtccccag tcagacaatc cgcaatatct aaaagtacat tctttagtca tcgagtctga
    29281 ccatcagaga cagggcttgg gaacacttct tcttgcagcc ttgaaagagg tggcagttga
    29341 gctggattac aaagggattc gtttggagag tcctgatgag ctgctttcct attttgaaat
    29401 gaacggtttt gttgatgaag aagcaacttt gctctatgca actagccagg gctatagtat
    29461 gatttggttt aatccctttt atctggagga acaatgaaaa tcagacaagc aagattagaa
    29521 gatttggatc ggattgttga acttgaattt gaaaattttt cggtcgaaga agccattcct
    29581 ccttctgtct ttgaagcaca tttgagagaa attcagacct cttttctggt tgctgaaaaa
    29641 gaaggaagaa tcatgggtta tatcgaagga ccagttggcc tgcaccgcca tctgcaagac
    29701 cagtctttta cagaagaaat aaaagactat agtcatgagc ctggtggtta tatctctgtg
    29761 acctgtctgt ctattgccaa ggaggcacag ggactcggac tgggtcagaa attgctgaca
    29821 gccttgaaag aagttgctct tgaagatgag agagatggca ttaatctaac ctgtcatgac
    29881 tatctcatcg cctactatga aaaacatgga tttgtcaacg aaggccagtc ccagtcaacc
    29941 tttgcagggg aaacatggta tgatatggtc tgggaaatga aaaaataagt taggaaaagt
    30001 atcatagatt gctttttttg gacttttaag atataatatt atggattatc aacaaggagg
    30061 aaaaactttt gagtgaaaag tcaagagaag aagagaaatt aagctttaaa gagcagattc
    30121 tgagagattt agaaaaagta aaaggctatg atgaagttct gaaagaagat gaggcagtag
    30181 ttcgcactcc tgcaaatgaa ccttcaactg aagaactcat ggctgattcc ttgtcaacgg
    30241 tagaggagat tatgagaaaa gctcctaccg tgcctactca cccaagtcaa ggtgtaccag
    30301 cttctccagc agatgagatt caaagagaaa ctcctggtgt tccaagtcat ccaagtcaag
    30361 atgtaccttc ttctccagcg gaagaaagtg gatcaagacc aggtccaggt cctgttagac
    30421 ctaagaaact tgaaagagaa tacaatgaaa ccccaacaag ggtagctgtt tcctatacga
    30481 cggcagagaa aaaagcagaa caagcaggtc cagaaacacc tacgcctgct acagaaacag
    30541 tggatatcat cagagataca tcacgtcgta gccgtagaga aggagcaaaa cccgttaagc
    30601 ctaagaaaga gaagaagtca catgtgaaag cttttgtgat ttcattcctt gtattccttg
    30661 ccttgctctc agcaggtggt tactttggtt accagtacgt gctagattcc ttattaccta
    30721 tcgatgctaa ttctaagaaa tatgtgacgg ttggaattcc agaaggttca aacgttcaag
    30781 aaatcggtac gacgcttgaa aaagctggtt tggtaaagca tggtctgatt tttagttttt
    30841 atgccaagta taaaaattat accgacttga aagcaggtta ctacaatttg caaaagagta
    30901 tgagtacaga agacttactc aaagagttgc aaaaaggtgg aacagatgaa ccgcaagaac
    30961 ctgtacttgc gactttgaca attccagaag gttatacctt ggatcagatt gctcaagctg
    31021 tgggtcaatt gcaaggtgac ttcaaagagt ctttgacagc ggagactttc ttggctaaag
    31081 ttcaagatga gacgtttatc agtcaagcag tagcgaaata tcctacttta ctggaaagtt
    31141 tgcctgtaaa agacagcggt gcgcgttatc gtttggaagg ataccttttc ccagctacat
    31201 actctatcaa ggaaagcaca actattgaga gcttgattga tgagatgtta gctgctatgg
    31261 ataagaacct atctccttac tatagtacta tcaaatctaa aaacttgact gtcaatgagt
    31321 tgttgaccat tgcttccttg gtcgaaaaag aaggtgccaa gacagaagat cgtaagctca
    31381 ttgcaggtgt attctacaat cgtttgaatc gtgatatgcc acttcaaagt aatattgcaa
    31441 tcttgtatgc ccaaggaaaa ctggggcaaa atatcagtct agctgaggat gttgcgattg
    31501 ataccaacat tgattcacct tataatgttt ataaaaatgt aggtctcatg cctggtccag
    31561 tcgatagtcc aagtctggat gcgattgagt caagcatcaa tcaaactaag agcgataacc
    31621 tctactttgt agcagatgtc acagaaggca aggtctacta tgctaacaat caagaagacc
    31681 acgaccgcaa tgtcgctgaa catgtcaaca gcaaattaaa ctaaacaaac taaaattatg
    31741 ttatacttca cataattttc tttagaaaat attatcagaa gaaagttgag aaaaatggca
    31801 gaaaaaacat atcctatgac ccttgaggaa aaggaaaaac ttgaaaaaga attagaagaa
    31861 ttgaaattgg ttcgtcgacc agaagtggta gaacgcatta agattgcccg ttcatacggt
    31921 gacctttcag aaaacagtga gtacgaagca gctaaggatg aacaagcctt tgtcgaagga
    31981 caaatctcta gcttagaaac aaaaatccgc tatgctgaaa tcgtcaatag cgacgcagtt
    32041 gcccaggacg aagtagcgat tggtaaaaca gtcaccatcc aagaaattgg tgaggacgaa
    32101 gaagaagttt atattatcgt aggttcagct ggtgcggatg cctttgcagg taaggtttca
    32161 aatgaaagcc caattgggca ggccttgatt ggcaagaaaa caggtgatac agcaaccatt
    32221 gaaacgcctg ttggtagcta tgatgtaaaa atcttgaagg ttgaaaaaac agcctaaaaa
    32281 cagaaaaagg agtggggagg cgatgtgctt cactcactcc tttttccatt ttgctattct
    32341 tcgaaaatct cttcaaacca cgtcagcgtc gccttgccgt atgtatggtt actgacttcg
    32401 tcagtttcat ctacaacctc aaaaccatgt tttgagc
//
LOCUS       DBERYS010000022        31858 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.022, whole genome shotgun sequence.
ACCESSION   DBERYS010000022 DBERYS010000000
VERSION     DBERYS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 31858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.022"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(21..956)
                     /gene="birA"
                     /locus_tag="ACL8LA_001138"
     CDS             complement(21..956)
                     /gene="birA"
                     /locus_tag="ACL8LA_001138"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGS1812902.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(953..1813)
                     /locus_tag="ACL8LA_001139"
     CDS             complement(953..1813)
                     /locus_tag="ACL8LA_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1812903.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRAGQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1921..4083
                     /locus_tag="ACL8LA_001140"
     CDS             1921..4083
                     /locus_tag="ACL8LA_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGS1812904.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4175..5434
                     /locus_tag="ACL8LA_001141"
     CDS             4175..5434
                     /locus_tag="ACL8LA_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1812905.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5448..6314
                     /locus_tag="ACL8LA_001142"
     CDS             5448..6314
                     /locus_tag="ACL8LA_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812906.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6328..7161
                     /locus_tag="ACL8LA_001143"
     CDS             6328..7161
                     /locus_tag="ACL8LA_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1812907.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7168..7335
                     /locus_tag="ACL8LA_001144"
     CDS             7168..7335
                     /locus_tag="ACL8LA_001144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812908.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKVKLTWFEEI
                     FEEYKKSSWNL"
     gene            7519..8961
                     /gene="gtfA"
                     /locus_tag="ACL8LA_001145"
     CDS             7519..8961
                     /gene="gtfA"
                     /locus_tag="ACL8LA_001145"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGS1812909.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9099..10292
                     /locus_tag="ACL8LA_001146"
     CDS             9099..10292
                     /locus_tag="ACL8LA_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS1812910.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10978..12957
                     /locus_tag="ACL8LA_001147"
     CDS             10978..12957
                     /locus_tag="ACL8LA_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1812911.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKTSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13024..14520
                     /locus_tag="ACL8LA_001148"
     CDS             13024..14520
                     /locus_tag="ACL8LA_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812912.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14520..15446
                     /gene="oppC"
                     /locus_tag="ACL8LA_001149"
     CDS             14520..15446
                     /gene="oppC"
                     /locus_tag="ACL8LA_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGS1812913.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15455..16522
                     /locus_tag="ACL8LA_001150"
     CDS             15455..16522
                     /locus_tag="ACL8LA_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812914.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16533..17459
                     /locus_tag="ACL8LA_001151"
     CDS             16533..17459
                     /locus_tag="ACL8LA_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1812915.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            17525..17656
                     /locus_tag="ACL8LA_001152"
     CDS             17525..17656
                     /locus_tag="ACL8LA_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812916.1"
                     /translation="MKNFESFLKEFLKNFKNYEMLLTGDEKGGRTGSIKKESRKRNE"
     gene            17949..18886
                     /locus_tag="ACL8LA_001153"
                     /pseudo
     CDS             17949..18886
                     /locus_tag="ACL8LA_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            18898..19791
                     /locus_tag="ACL8LA_001154"
     CDS             18898..19791
                     /locus_tag="ACL8LA_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1812917.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLTGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            19804..20607
                     /locus_tag="ACL8LA_001155"
     CDS             19804..20607
                     /locus_tag="ACL8LA_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1812918.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20640..21350)
                     /gene="treR"
                     /locus_tag="ACL8LA_001156"
     CDS             complement(20640..21350)
                     /gene="treR"
                     /locus_tag="ACL8LA_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGS1812919.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21535..23502
                     /gene="treP"
                     /locus_tag="ACL8LA_001157"
     CDS             21535..23502
                     /gene="treP"
                     /locus_tag="ACL8LA_001157"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGS1812920.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23680..25305
                     /gene="treC"
                     /locus_tag="ACL8LA_001158"
     CDS             23680..25305
                     /gene="treC"
                     /locus_tag="ACL8LA_001158"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGS1812921.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25561..25809
                     /locus_tag="ACL8LA_001159"
     CDS             25561..25809
                     /locus_tag="ACL8LA_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGS1812922.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26016..26810
                     /gene="racE"
                     /locus_tag="ACL8LA_001160"
     CDS             26016..26810
                     /gene="racE"
                     /locus_tag="ACL8LA_001160"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGS1812923.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26807..27778
                     /locus_tag="ACL8LA_001161"
     CDS             26807..27778
                     /locus_tag="ACL8LA_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGS1812924.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27754..28275
                     /locus_tag="ACL8LA_001162"
     CDS             27754..28275
                     /locus_tag="ACL8LA_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGS1812925.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28272..28733
                     /gene="cbpB"
                     /locus_tag="ACL8LA_001163"
     CDS             28272..28733
                     /gene="cbpB"
                     /locus_tag="ACL8LA_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGS1812926.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28730..29464
                     /gene="xerD"
                     /locus_tag="ACL8LA_001164"
     CDS             28730..29464
                     /gene="xerD"
                     /locus_tag="ACL8LA_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGS1812927.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29464..30192
                     /locus_tag="ACL8LA_001165"
     CDS             29464..30192
                     /locus_tag="ACL8LA_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGS1812928.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30211..30780
                     /gene="scpB"
                     /locus_tag="ACL8LA_001166"
     CDS             30211..30780
                     /gene="scpB"
                     /locus_tag="ACL8LA_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGS1812929.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30767..31489
                     /locus_tag="ACL8LA_001167"
     CDS             30767..31489
                     /locus_tag="ACL8LA_001167"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1812930.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31489..31731
                     /gene="yidD"
                     /locus_tag="ACL8LA_001168"
     CDS             31489..31731
                     /gene="yidD"
                     /locus_tag="ACL8LA_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGS1812931.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
BASE COUNT         9238 a         5922 c         7047 g         9651 t
ORIGIN      
        1 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
       61 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      121 gcaagtccct tgtagtcttt ttgctgtagt gtgaaagtga cttcttttcc tagaatgaat
      181 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      241 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      301 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      361 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      421 ttatgattgt ttagatagat atcattgacc cattttatgt cgacatctat taaagttagg
      481 ttcttaatag ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      541 taggggagat ttggtttaag atggagtgtc atataaatac caccttgtgg tgagtagaag
      601 gaacgttgaa aacgtcctcg gcctgctgtt tgataggaag ctagatagag ggtatttgct
      661 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      721 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      781 attagtttat atcctctatt tttgatacta tcaatttcaa tgccttcttg ttctagtcgc
      841 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
      901 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
      961 ttgcctttct cactagttgg tattgagagt attcttttct tgtatgactt ggagtctgat
     1021 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1081 caattacctt gatggactct tgggtatttt cgagaagttg tccagctcgg tgcattcgaa
     1141 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1201 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1261 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1321 ttttctgatt gggagcaata gttcccagat gaaacatcag ttcatgaagt tgtcccatga
     1381 tatggagttg agctaattca cttgattttg taatctgagc gaagcggaca atgtctgaga
     1441 tgagttttgc agtagtctgg gtatgacaag tttcagattg gatgagatag gattgatcag
     1501 aaatttggga aagagcaaaa taatcagggg ctttccctcc agtgattcct aaccagtagt
     1561 aggcccaagg ttctttacta tctgcttgat aaaaggttag ttcctctggt tttaatagaa
     1621 agaaatctcc ttcttttaaa tcaacaattt tacccttgta atgaaatttt ccttgtcctt
     1681 tagtaatgta atgtagaacg tatgtatcac gaatggctgg accaaaagag taattaggtg
     1741 tgcattcctc atatccataa aagcttaggg caaggtcgat tgttccagtc tggtattctg
     1801 aaaaaactaa catgtgctac ctcctaccta acattttacc atattcttga gacatttttc
     1861 tattttggaa agcgatttca ggtgataaaa tataatcaat aaagtgatga ggtgaagtaa
     1921 atgggagtta ggatagagaa taatctattt tatgttgaga gtaaaaatct aagtttgatt
     1981 attgaaaatc gaaatggcta cttacttttg aaacatttag gaaagactat taagaactat
     2041 aaaggttcca atagtgttta tgaacgagac catgcctttt caggaaatcc aacggctact
     2101 aatcgaacct ttagtttaga tactcaacgt caaatttttg gacaacatgg cttaggagat
     2161 tttaggaaac caaccataca ggttcagcat aatgtaactg aagtaacaga ctttcgattt
     2221 gtagaagcaa agattttaaa aggtcagaat ggtccacagg gcttaccttc tccacatagc
     2281 atggacgata cagagactct tgtcttaatg ttagaagatt ctaaggctca acttagtctg
     2341 actttgtatt atactacttt taataatgat gcgactattg ctagctacag taaattagat
     2401 aataatagta atcaggaagt tgtcatccat aaagattttt cttttatggc tgattttcca
     2461 gctgcagatt atgaaatagc aactctgcag ggtgcttatg ctcgtgaaaa gactgttaga
     2521 cgtcaacagg tagaacaagg aatcttttcg attagttcaa accgaggtgc ttctggtcat
     2581 gctcaaacac cagctcttct actatgcgaa caaggagtca cagaggatgc tgggaatgtg
     2641 tttgctattc aactaatgta tagtggcaac tttgaagctt ttgttcaaaa aaatcaattg
     2701 aatgaagttc gggtggctat tggcattaat ccagaaaact tttcttggaa gttagcccct
     2761 gaggaatact ttgaaacacc ggtagcttta gtgactcatt cagatcaggg attaactggt
     2821 attagtcatg aaagtcagaa ttttgtactg aagcacatta tgctaagtga attttctaaa
     2881 aaagaacgtc caattctaat caataactgg gaagctactt actttgactt tcagagagaa
     2941 aaactgttag agttagcaga tgaagctaag aaagttggca ttgaactttt tgtattagat
     3001 gatggttggt ttggcaatcg ttttgatgat aatcgtgctt taggtgattg ggttgttaat
     3061 gaggaaaaac tgggtggaag tctagaaagt ctgatttcag ctatccatga aagaggtttg
     3121 cagtttggac tttggttaga acccgaaatg atttctgtag atagtgattt gtatcgtcaa
     3181 catcctgact gggctattca ggttcctgat tatgagcata cttattctcg gaatcaatta
     3241 gtacttaatc ttgccaatcc tcaggtagta gaatacttga aaagtgtctt agatcaactc
     3301 ctatcttatc atgagattga ttacattaaa tgggatatga accgcaatat cactaagcta
     3361 gggaatggat taacttatct agagacacag atgcaatctc atcagtacat gctggggctt
     3421 tacgaactcg tttcttatct gacagagaag cacagccata ttctctttga gtcctgctct
     3481 ggtggtggtg gacgaaatga tcttggtatg atacgctatt tcccacaagt ctgggctagt
     3541 gataatactg atgccattgc acgtttacca attcaatacg gttcatccta tctctatcca
     3601 accatttcta tgggggctca tgtgtcagca gtaccgaatc atcagatggg acgaatgaca
     3661 ccattagaaa cacgtggcca tgtagcaatg atgggaaatt tgggctatga gcttgatttg
     3721 acaaatttat cagatgaaga gaaagctacg attgctaatc aggtgaactt gtataaagaa
     3781 ttacgaccag tagttcagtt aggacagcag tatagactaa ttaatcctga tactgcatcc
     3841 aatgaagcag ctgtacaatt taattacgga aatcaaacga ttgtaaccta cgttcgcgtt
     3901 ttatctgttg tagagaccat ggaaacaact ttaaaattaa aagatttgga tgaagaggga
     3961 ctatataaat tacaggaaaa tggcgaagtt tactcaggtg cagaactaat gtatgcgggc
     4021 ttaactgtta tcttatccca aggagatttt ttgagtagac agtatatttt tagaaaacta
     4081 tgatatgtac tgaccccaaa agttagattt attctgtcta acttttgggg tgcagttcaa
     4141 taagggtgta taaatttata aaggaggctt tctaatggaa tggtataaaa aaatcggact
     4201 tcttgcaact acaggtttag ctttgtttgg gctcggcgct tgctccaact atggtaaatc
     4261 tgcggatggc acagtgacca tcgagtattt caaccagaaa aaagaaatga ccaaaacctt
     4321 ggaagaaatc actcgtgatt ttgagaagga aaaccctaag atcaaggtca aagtcgtcaa
     4381 tgtaccaaat gctggtgaag tattgaagac acgcgttctc gcaggagatg tgcctgatgt
     4441 ggtcaatatt tacccacagt ccatcgaact gcaagaatgg gcaaaagcag gtgtttttga
     4501 agatttgagc aacaaagact acctgaaacg cgtgaaaaat gcctacgctg aaaaatatgc
     4561 tgtaaacgaa aaagtttata acgttccttt tacagctaat gcttatggaa tttactacaa
     4621 caaagataaa ttcgaagaac tgggcttgaa ggttcctgaa acctgggatg aatttgaaca
     4681 gttagtcaaa gatatcgttg ctaaaggaca aacaccattt ggaattgcag gtgcagatgc
     4741 ctggacactc aatggttaca atcaattagc ctttgcgaca gcaacaggtg gaggaaaaga
     4801 agcaaatcaa taccttcgtt attctcaacc aaatgccatt aaattgtcgg atccgattat
     4861 gaaagatgac atcaaggtca tggacatcct tcgcatcaat ggctctaagc aaaagaactg
     4921 ggaaggtgct ggctataccg atgttatcgg agccttcgca cgtggggatg tcctcatgac
     4981 accaaatggg tcttgggcga tcacagcgat taatgaacaa aaaccgaact ttaagattgg
     5041 gaccttcatg attccaggaa aagaaaaagg acaaagctta accgttggtg cgggagactt
     5101 ggcatggtct atctcagcca ccaccaaaca tccaaaagaa gccaatgcct ttgtggaata
     5161 tatgacccgt ccagaagtca tgcaaaaata ctacgatgtg gacggatctc caacagcgat
     5221 cgaaggggtc aaacaagcag gagaagattc accgcttgct ggtatgaccg aatatgcctt
     5281 tacggatcgt cacttggtct ggttgcaaca atactggacc agtgaagcag acttccatac
     5341 cttgaccatg aactatgtct tgaccggtga taaacaaggc atggtcaatg atttgaatgc
     5401 cttctttaac ccgatgaaag cggatgtgga ttaggagtag agagactatg aaaaaagtat
     5461 tacaaaaata ttgggcatgg gcttttgtgg tcatccccct cttgttacaa gcaattttct
     5521 tctatgtgcc gatgtttcaa ggagcctttt acagttttac caactggaca ggattgactt
     5581 acaactacaa atttgtcggc ttaaacaact ttaagctcct cttcatggat ccaaaattca
     5641 tgaatgcgat tggctttacc gcaatcatta cgattgccat ggtggttggt gagattgcac
     5701 tcgggatctt cattgcgcgt gtcttgaact ctaaaatcaa aggccaaacc ttcttccgtg
     5761 cttggttctt cttcccagct gttttatctg gtttgacagt ggctttgatc ttcaagcaag
     5821 tcttcaacta cggtcttcca gcgattggga atgcccttca tattgaattt ctccaaacca
     5881 gtcttttagg gactaagtgg ggagcaatct ttgcggctgt ctttgtcctt ctttggcaag
     5941 gggtggctat gcccatcatc atcttcctag ctggtttgca atctattcca actgagatta
     6001 cagaggcagc aaggattgat ggtgcgacta gcaagcaagt tttctggaac attgaattgc
     6061 cttacttgct accaagtgtc tctatggtct ttatcctagc cctaaaaggt gggctgactg
     6121 cctttgacca agtctttgcc atgaccggtg gtggtccaaa caatgccaca acctcacttg
     6181 ggctcttggt ttataactat gcctttaaaa acaaccaatt cggttatgcc aatgccattg
     6241 ccgtaatctt gttcctctta attgtagtga tttcgatcat ccaattgaga gtatctaaga
     6301 aatttgaaat ttaagaggag aagcatgatg aaacaagatg aaagaaaagc cctgattggc
     6361 aaatacattc tattgattct aggatcggtt ctgattttag tgccgctcct tgctaccctc
     6421 tttagttcct tcaaacccac taaggatatt gtagataatt tctttggctt tccaaccaac
     6481 ttcacatggg acaactttag ccgtctctta gctgatggga ttggaggcta ttattggaac
     6541 tctgtcgtca tcactgtctt gtctttactt gcagtaatga tctttatccc tatggcagcc
     6601 tactccatcg ctcgcaatat gagtaaaaga aaagtcttta ccatcatgta taccctctta
     6661 atcctcggaa tcttcgtacc tttccaagtc atcatgattc cgattacggt tatgatgagt
     6721 aaactcggtt tggctaatac ctttggtttg atcttgctct acttgaccta tgcgattcca
     6781 cagaccctct ttctctatgt tggctatatc aaaatctcga ttccagaaag tctggatgaa
     6841 gcagcagaga tcgatggggc taatcaattt acaacctatt tccgcatcat cttcccaatg
     6901 atgaaaccga tgcatgcgac aaccatgatc atcaatgccc tttggttctg gaatgacttc
     6961 atgttgccac tccttgtctt gaaccgggat tccaaaatgt ggactctgcc tttgttccaa
     7021 tacaactacg caggccaata tttcaacgac tacggaccaa gctttgcctc ttacgtggtc
     7081 ggcattatca gtatcaccat tgtctatctc ttcttccaac gccatatcat ttcaggaatg
     7141 agcaacgggg cagtgaagta atactcaatg aaaatcaaag agcaaactag gaagctagcc
     7201 gcaggttgct caaaacactg ttttgaggtt gtggatgaaa ctgacgaagt cagtaaccat
     7261 acctacggta aggtgaagct gacgtggttt gaagagattt tcgaagagta taagaagagc
     7321 tcatggaatc tatagtcttt gacacagagg aagagttcag cttgttgata attctatgta
     7381 gaaaacaaga aagtagtact ttgtttatca cagtctatat tagtacgagt ttgatgataa
     7441 atagagattt aaaatgaatt tgatttacga agccagatca tatgagactt ggcttcaatt
     7501 agaaaaagga gagtaatgat gccaattcaa aataaaacca tgttgattac ctattctgat
     7561 agccttggaa ataatcttaa agacttatat gataatttgg aagagcattt tggagatgct
     7621 attggaggag ttcacctttt accatttttc ccatcaacag gtgatcgtgg atttgcgcca
     7681 gttgactacg acgaagtgga ctcagctttt ggtgattggg aggatgttaa gcgtttaggt
     7741 gagaaatatt atcttatgtt tgattttatg attaatcata tttctcgtca atccaagtat
     7801 tataaggact atcaagaaaa acatgaagcc agtgaattta aagatctctt tttaaactgg
     7861 gataagtttt ggccagaaaa ccgtccgaca cagtctgatg tagatttaat ttacaagcgt
     7921 aaggatcgtg caccaaagca agagattgtg tttgaagatg gttcagtgga acatttgtgg
     7981 aatacctttg gtgaggagca gattgatctt gatgtgacca aagaagtaac tatggaattt
     8041 atccgtaaga ccattcagca cttggcaagt aatgggtgtg atttgattcg tctagatgcc
     8101 tttgcttatg cagtgaagaa attggatact aatgatttct ttgtggaacc agatatttgg
     8161 gatttattgg acaaagttcg agatatcgct gctgagtatg ggacagagct tttacctgag
     8221 attcatgaac actattcgat tcagtttaaa atagcagacc atgattacta tgtttatgat
     8281 tttgctcttc caatggtgac actttatact ctttacagtt ccagaacaga gcgtttggct
     8341 aagtggttaa agatgagccc gatgaagcaa tttacgacgc tagataccca tgatgggatt
     8401 ggagtagtag atgtcaagga tatcctgacc gatgaggaga ttgactatgc ttcaaatgaa
     8461 ctctataagg ttggagccaa tgtcaaacgt aagtactcta gtgccgagta taacaactta
     8521 gatatctacc aaatcaattc aacctactat tcagcgcttg gagatgatga tgtcaagtat
     8581 tttctcgctc gtctaattca agcttttgcc ccaggtattc ctcaggttta ctatgtgggt
     8641 ctattagcag gcaagaatga cttgaaatta ttagaagaaa ctaaagaagg tcgaaatatt
     8701 aatcgtcatt actatagcaa cgaggaaata gcaaaagaag tgcaacgacc tgttgtgaag
     8761 gcccttctca atctattttc tttccgtaac cgttcagaag cctttgatct agaagggact
     8821 actgagatag agacaccaac agctcacagc attgtaatca aacgtcaaaa taaagataag
     8881 tccgtaacag cagtagcaga aattgacttg caaaatcaga cttatcgggt aattgagaat
     8941 ggagttgaag taacattttg aagccttgat atggtggata aaatagggtt tttattttgg
     9001 aaaacgtttc ctttgttttc aaattgctaa aaaagtggta caatatagga tagcttacta
     9061 ttatctgaat cagctgattt ggagagaaag gattcatttt gaaatcaata ggctttattg
     9121 aaaagctgaa ggggttgtct agtaaagagc tgattttatt gggaattatc ctgagtatct
     9181 ttttaccctt ttatcttttt gtagttgtat tctgtttata tattatcagt ctgattttta
     9241 caggagacat gaaaagtatt cttcagaaaa tgggggagca ttcgatgctg cttctttttc
     9301 ttggctatag tactgttata tccattcttg cacaaaattg gatgggtctt gtggcttcag
     9361 taggaatgtt tctatttact attttctttt tgcactatca gtcgatttta tcccataaat
     9421 tctttcgatt gattttgcag ttcgtcttgt ttggtagtgt cttgtcagct gcttttgcca
     9481 gtttagaaca tttccaaatt gtgaagaaat ttaactatgc ttttctttcc cccaatatgc
     9541 aggtgtggca tcagaaccgg gcagaagtga ccttctttaa tcctaattat tatggaatta
     9601 tttgttgttt ctgtattatg attgctttct atctgtttac aacgaccaag ttgaattggt
     9661 tgaaagtatt ctgtgtgatt gcaggctttg ttaatctctt tggtttgaac tttactcaaa
     9721 atcgaactgc ctttcctgct attatcgctg gagcaattat ctatctcttt acgactatta
     9781 aaaactggaa ggccttttgg cttagtattg gggtcttcgc gattggtttg agtttcctct
     9841 tttctagtga tttgggagtt cgaatgggta ctttagactc ttctatggaa gaacgcattt
     9901 ctatctggga tgctgggatg gccttgttta agcaaaatcc tttttggggt gaagggccat
     9961 tgacctatat gcactcttat cctcggatac atgctcctta tcatgaacat gcccacagtc
    10021 tttatattga tacgattctg agttacggaa ttgtgggtac cattttatta gttctgtctt
    10081 ctgttgctcc tgttcgcttg atgatggata tgagtcagga gtcggggaaa cgtccgatta
    10141 tcggccttta tctatctttc cttacagtgg ttgctgtgca cggaattttt gacttggctc
    10201 tcttctggat tcagtcaggc tttattttct tgctagttat gtgcagtatt ccattggagc
    10261 atcgaatgtt ggtatcggac atgacggatt aagttttata agtttgaaat cttctacctt
    10321 gggtaggaga ttttttatac gatgggcatg tcgtgcatct gaggtgtaag tcctctgtag
    10381 ggatccacta ccggtgaata caatagcgct ccccaagcct gactatcgtg aggtagcgga
    10441 ttaaaatggt ctggggatag atcgttttaa gtctgacgca ggaaataaga attgtcagag
    10501 aaagcaatag tgaaatcgta ggctatgaat agaaacgtga tagcgaggag catatagtgg
    10561 ataaggtagc gagaaatttt aaagtataaa agatagtctt aatctatata tgtaaatcac
    10621 aaaagtaatt gaattcggaa taaggttggt gtgaaaagac atagtaaact gaatcttgaa
    10681 tactagaatg atacttctac aatattgtta taatttaatt tgaattccaa aatcacattg
    10741 ttccgtccgt atttcatttt actgtatgtc ttcctaaact ccaaagtttc tgcgtcaaat
    10801 ttcctatttt cactgtagtc tttgacactt cgaacgacaa tttgaatcat atattcaaaa
    10861 aaaaatgaaa aatttgtgcc aacaagccta aatattctga aaatttgttt gatttttaat
    10921 ggataatgtg atataatggt tgtcaatcta taattcacat aaacagagga gtagaaaatg
    10981 aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat tagcagcagg tgtacttgca
    11041 gcatgcagtt cttcaaaaac atctgattca tcagccccta aagcttatgg ctatgtttat
    11101 acagcagacc cagaaacctt ggactacctg atttcaagta aaaatagtac aacagtagtg
    11161 acttcaaatg ggattgatgg tttattcact aacgataatt acggtaatct tgctcctgca
    11221 gttgcagagg attgggaagt ctctaaggat ggtttgacct acacttataa gattcgtaaa
    11281 ggggttaaat ggtttacctc tgatggagaa gaatatgcag aggtgacggc taaagatttc
    11341 gtgaacggtt taaaacacgc agcagataaa aaatcagaag ctatgtattt agctgaaaat
    11401 tcggttaaag gcttggcaga ttatctatca ggaacttcaa cagatttttc aacagttggt
    11461 gtcaaggcgg ttgatgatta tacgttacaa tacactttga accagcctga accgttctgg
    11521 aactctaagt tgacctattc tattttctgg cctctgaatg aagaattcga aacatcaaaa
    11581 ggaagcgatt ttgctaaacc aacagatccg acatccttgc tttataatgg tccattcttg
    11641 ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa aaaatgagca atattgggat
    11701 aaagaaaatg tccacctaga tactatcaat ctagcttact atgatggatc agatcaggag
    11761 tcgctagagc gtaacttcac tagtggagct tatagttatg cccgtcttta ccctaccagc
    11821 tccaactatt ctaaggttgc agaagaatac aaggacaata tctattacac acaatcaggc
    11881 tctgggattg ctggtctggg tgtgaatatt gatcgccaaa gttacaacta tacttctaaa
    11941 actacagatt cagagaaagt agctactaag aaggcattgc ttaacaaaga tttccgtcaa
    12001 gccttgaatt ttgctcttga tcgctcagct tactcagctc aaatcaatgg taaagatgga
    12061 gcagctttag cagttcgtaa tttatttgta aaaccagact ttgtttcagc tggtgagaag
    12121 acctttggtg atttagtcgc tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg
    12181 aatttagctg atgggcagga tggtttattc aatgctgaca aggccaaggc agagtttgcg
    12241 aaagctaaga aagctttaga agcagacggc gttcagtttc ctattcatct ggacgttcca
    12301 gtagaccaag catcaaaaaa ctatatatct cgtattcagt cctttaaaca atctgtagaa
    12361 acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac aaatgacaag tgatgaattc
    12421 cttaatatta cttactatgc tgccaatgct tcatctgagg attgggatgt atcgggagga
    12481 gtttcatggg ggccagacta tcaagaccca tctacttacc tggatatttt aaaaacaact
    12541 agcagtgaaa ctacaaaaac atatttagga tttgataatc caaatagccc ttcagtagtt
    12601 caagttggtt tgaaagaata cgataaatta gttgatgaag ctgccaaaga gacaagcgac
    12661 ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat ggttgacaga tagttcactc
    12721 tttattcctg ctatggcttc ttctggtgca gcaccagtgc tttcacgaat tgttccattt
    12781 actggagctt ctgcgcaaac aggctctaag gggtcagatg tttacttcaa atatttgaaa
    12841 ttacaagata aagcggtgac taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa
    12901 gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg caagtcatgt gaagtaaata
    12961 atttctaata gaactttctc tccatgagga gaaggttctt ttgggatttt taaaggaaat
    13021 aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta
    13081 acgactttga cctacacgat tatctatacc ttggttcctc gaaaattgat tttcaagcag
    13141 gatcctaact ataataaaat tgcgacaacg gctgataaac gtgataacta tgaaaatact
    13201 gtgtttgagc gtatgggcta cattgagtat tacgatacta aagagttgca agaaaaggca
    13261 agtagcatgg attcttctgt aacagtagaa gcaaatgcga ccaataaagc tatttatgaa
    13321 aagtacatca atcaattagg tcatggttgg actttgggag aatttactga aagtggtcaa
    13381 ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt ttcacttcta tgctaacttg
    13441 attgacattg accatacaaa taaaatccaa gaccctgaaa atccagactt gaaacgctac
    13501 cttcgttttg aaaacgatcc agctatcgga tggtcattgg tcggttcagg aactaaacat
    13561 aaatatctct tgtactttaa cagtcagttc ccatttgttc atcaaaactt tgtgaacttg
    13621 aatttaggtg actcttaccc aacctatgct aatacaccag ttcttcaggt tattactcaa
    13681 ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa caggtaagaa aacgtcttct
    13741 gtaaatattt actcaagaac ctacaagtca cctagtcagg ctgactctcg tgaagtagct
    13801 agctatggga aagatgatcc ttatacagcg actgaaagta attaccaata tccatctatg
    13861 attgtcagct ctgctattac tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg
    13921 ccacttggtt cagccatggc tcgtttcaag aacacttgga ttgatagcct ctcaacaggg
    13981 gctttgacct tcttgcttgc tcttccaacg attgccttgg tttacatcgt tcgattgatt
    14041 ggatcatcta tttcccttcc agattcattc cctatcttgg gagctggaga ttggcgttct
    14101 tacgttttac cagcagtcat ccttggtttg ttgggtgctc ctggtacagc catttggatt
    14161 cgtcgttaca tgattgactt gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt
    14221 ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa atgccatggt tccgctggtt
    14281 tcaggaattc ctggtgccat tattggggtt atcggtggtg caacccttac tgaaacagtc
    14341 ttcgccttcc caggtatggg taaaatgttg attgactctg taaaagcatc taataactct
    14401 atggtcgttg gtcttgtctt catctttaca tgtatttcta tcttctcacg tcttttggga
    14461 gatatttgga tgactattat tgacccacgt attaaattga ctgagaaagg aggcaaataa
    14521 tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa
    14581 ctattgatgc gccagcatat tcttactgga aatcagtgtt taaacaattt atgaagaaaa
    14641 aatcaactgt agtcatgttg ggaatcttgg tagccatcat tttgataagt ttcatctacc
    14701 caatgttttc taagtttgat ttcaatgatg tcagcaaggt aaacgacttt agtgttcgtt
    14761 atatcaagcc aaatgcggag cattggttcg gtactgacag taacggtaaa tcgctctttg
    14821 acggtgtctg gttcggagct cgtaactcca tcctcatttc tgtgattgcg acagtgatta
    14881 acttggttat cggtgttttt gtcggtggta tttggggtat ttcaaaatca gttgaccgtg
    14941 tcatgatgga agtttacaac gtcatctcaa acatcccacc tcttttgatt gttattgtct
    15001 tgacttactc aatcggagct ggattctgga atctgatttt tgccatgagc gtaacaacat
    15061 ggattggtat tgccttcatg atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca
    15121 acttggcgtc acgtactttg ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc
    15181 ctcaattggt atctgttatt gtgacaacca tgactcaaat gcttccaagc tttatctcat
    15241 acgaagcctt cttgtctttc ttcggtcttg gattaccgat tacagtgcca agtttgggtc
    15301 gtttgatttc ggattattca caaaacgtaa caaccaatgc ttacttgttc tggattccat
    15361 tgacaaccct tgtcttggta tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg
    15421 ctagtgatcc acgtacacat agataggagt agaaatgaca aaagaaaaaa atgtaatttt
    15481 gactgctcgc gatattgtcg tggaatttga cgttcgtgac aaagtattga cagccattcg
    15541 cggcgtttcc cttgaactag tcgaaggaga agtattagcc ttggtaggtg agtcaggatc
    15601 aggtaaatct gttttgacaa agaccttcac aggtatgctc gaagaaaatg gtcgcattgc
    15661 ccaaggtagt attgactacc gtggtcagga cttgacagct ttatcttctc acaaggattg
    15721 ggaacaaatt cgtggtgcta agattgcgac tatcttccag gacccaatga ctagtttgga
    15781 ccccattaaa acaattggta gtcagattac agaagttatt gtaaaacacc aaggaaaaac
    15841 agctaaagaa gcgaaagaat tggccattga ctacatgaat aaggttggca ttccagacgc
    15901 agatagacgt tttaatgaat acccattcca atattctgga ggaatgcgtc aacgtatcgt
    15961 tattgcgatt gcccttgcct gccgacctga tgtcttgatc tgtgatgagc caacaactgc
    16021 cttggatgta actattcaag ctcagattat tgatttgcta aaatctttac aaaacgagta
    16081 tcatttcaca acaatcttta ttacccacga ccttggtgtg gtggcaagta ttgcggataa
    16141 ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga acggttgagg aagtcttcta
    16201 tgaccctcgc catccatata catggagtct cttgtctagc ttgcctcagc ttgctgatga
    16261 taaaggggat ctttactcaa tcccaggaac acctccgtca ctttatactg acctgaaagg
    16321 ggatgctttt gctttgcgtt ctgactacgc aatgcagatt gacttcgaac aaaaagctcc
    16381 tcaattctca gtatcaaaga cacattgggc taaaacttgg cttcttcatg aggatgctcc
    16441 aaaagtagaa aaaccagctg tgattgcaaa tctccatgat aagatccgtg aaaaaatggg
    16501 atttgcccat ctggctgact aggaggaagg aaatgtctga aaaattagta gaaatcaaag
    16561 atttagaaat ttccttcggt gaaggaagta agaagtttgt cgcggttaaa aatgctaact
    16621 tctttatcaa caagggagaa actttctcgc ttgtaggtga gtccggtagt gggaaaacaa
    16681 ctattggtcg tgctatcatc ggtctaaatg atacaagtaa tggagatatc atttttgatg
    16741 gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc ggaattgatt cgtcgaatcc
    16801 agatgatttt ccaagaccct gccgcaagtt tgaatgaacg tgcgactgtt gattatatta
    16861 tttctgaagg tctttacaat caccgtttat ttaaggatga agaagaacgt aaagagaaag
    16921 ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca cttgactcgt taccctcatg
    16981 aattctcagg cggtcaacgt caacgtatcg gtattgcccg tgccttggtc atgcaaccag
    17041 actttgttat tgcagatgag ccaatttcag ccttggacgt ttctgtacgt gcccaagtct
    17101 tgaacttgct caaaaaattc caaaaagagc tcggcctgac ctatctcttc atcgcccatg
    17161 acttgtcggt tgttcgcttt atttcagatc gtatcgcagt tatttacaag ggtgttattg
    17221 tagaggttgc agaaacagaa gaattgttta acaatccaat tcacccatat actcaagcct
    17281 tgctttcagc ggtaccaatc ccagatccaa tcttggaacg taagaaggtc ttgaaggttt
    17341 acgacccaag tcaacacgac tatgagactg ataagccatc tatggtagaa atccgtccag
    17401 gtcactatgt ttgggcgaac caagccgaat tggcacgtta tcaaaaagga ctaaactagt
    17461 aatggtttta taatttccat gtcaattttt atggaaatta taaaactttt tttgttggac
    17521 tgatttgaaa aactttgaaa gcttcctgaa agaatttttg aaaaatttta aaaattatga
    17581 aatgttattg acaggtgatg aaaaaggtgg taggacagga agcatcaaaa aagaaagcag
    17641 aaaaagaaat gaataagaga caagccgtaa ctatgaatca aaacttttac tttagctact
    17701 ttagatagtg tttgtagaca tgtcaccatg gatgcaaaca gttcaagcaa tttgtttgta
    17761 tctatgtggc taagattttt gattgtggtc catatagatg aatatctaaa tggactagct
    17821 aagttgtaac ttgttaggtt atttcaagta tccgtttaga tagtatctag gcggtttttt
    17881 gttttatctt ttctgagaag gagtttcatt atgaaagttg ttcgaaaatc actagccccc
    17941 tcttggtagt ggggctcctc ttgacttctc tgatcagttt gcatcagttg aaggcagata
    18001 agaaaaagat gtctttcgta tcggtatttc gcaatttatt acccaccagt ccttatacgc
    18061 tactagagaa ggatttgtgg atgagctggc caaacaaggc tatgttgagg gggaaaatat
    18121 tgagattgat ttgcaaaatg cacagggaga acaaagaaat ctaaaaacga tttcccagca
    18181 actagcagaa tccagcgatg tcgttctagc catcgcaagg ccttctgctc agagcttggc
    18241 caatacgacg caaacgacac cagttatctt ttcagctgta acagacccag tcagtgccaa
    18301 gttggttgag tcaagagaac atcctggggg caatgtaact gggacaagcg atcagtcatc
    18361 agatgccatt tcaacccaaa tcaatttgat aaagaaagtg ttgccaaagg ctaaaacgat
    18421 tggaatcctc tatactcaga gcgagcccaa ttcggttgtt caaaaggatg aagctaagcg
    18481 acttctgaaa gaaaaaggct ttaccgttgt tgaaaaaaca atcttggaca gtaataacgt
    18541 caaggcggca gcagagagtt tgatggcaga ggtggatatg gtttttgtcc caactgataa
    18601 tatcatttcg ttaaccatgg aaacagttaa gcaggtttct attaaacaca aggttccagt
    18661 attcggtggt tcaacagaaa tgattgctgt tggtggcttg tataactacg gaaccaatta
    18721 tgaagaattg gtacgtcaga cagctcgcat gctgattcgt gttttaaaag gtgagaaacc
    18781 agaaaatata gcagttgagt tgcctgaaaa actggaattg cataccaatc aagaaatggc
    18841 agatgcattg ggaattgata ttagtaagtt agaaggcaag gaataaggag gattaagatg
    18901 aattttgtgt tatctagttt atcagaaggt ttgttgtggt cgattgtggc gattggggtc
    18961 tacttgactt tccgtatttt ggatattgcg gatatgactg ctgagggagc ctttcctctg
    19021 ggggcagcag tcgttgtatc acagatacag gcaggggcaa atccttggct tgcgacttta
    19081 cttgctttgc tgacaggtat ggtagcaggt cttgtatcag gaatgctcca caccaagatg
    19141 aaaatcccag ctctcttgac agggattgtg accttgacag ggctctattc aatcaatatt
    19201 aaaatcatgg gcagtgtacc caatctttcc ttgggagatt ctgcaaccgt ctttaaacag
    19261 ttggcgagct tgggtctgac aaatgaagga actgttttct cacttagttt ggcctgtttt
    19321 ttacttgttt gtttggtctt gacccttttg atgaaaacag agattggcct ggtcttgcgt
    19381 tcgactgggg ataatatccc gatgagtgag gccaatggag tcaatgtaga caccatgaag
    19441 attgttggtt acatgatttc aaacggtttg attgctctat gtggttcctt gtttgcccaa
    19501 aatgatggat tttcagatgt gacttctggg acaggaacca ttgttgttgg tttgagctcg
    19561 gtgattattg cggaagtctt gatacacgac ttgaccattg gaggccgctt attatccatc
    19621 ggaatcggtg ctattgttta ccgtttgatt attttaaata tctatgaaat tccaaatcta
    19681 gatcaaaact tggtacgtct ctttaatgca atcttgctcg ctttggtttt atttgcacca
    19741 gaattgcaaa agagattaaa gattcgtggt ctgaaattga gaaatgaata ggaggagaaa
    19801 gttatgacaa gtttgttaac acttgaaaat attcacaaga catttgaagc agggacggtc
    19861 aatgagaacc atgtcctcaa aggattagat ttagaggttg aagagggaga ttttatctct
    19921 gtgattggtg gaaatggagc aggaaaatcc actttgatga atatcttggc tggcaatcta
    19981 tcggtggacg aaggggacct tctactggca gggaaatcca ttaaaaatct gagtgtcaga
    20041 aagagagcca aggatattgc ccgtgttttt caagatccta agatgggaac agcttctcgt
    20101 ttgacgattg aggagaatat ggctattgcc ttgagacgcg ggcagaagag agggcttggt
    20161 tggggcgtga aggagaagga tagaatccag ttccaagagg ccttgaagga gttgaatatt
    20221 ggtcttgaaa accgcttaaa agtagatact caatatctct caggaggaca aagacaggct
    20281 ttgactctag tcatgacaga tctgatgaaa ccaaaacttt tacttttgga tgaacatact
    20341 gcggcacttg acccgaaaac cagtcaaatg gtgatggact tgacgcaaaa gattgtggag
    20401 caccatcagt ggacgacttt gatgattacc catgatatga atcatgcgat tgagtacggc
    20461 aatcgtctca ttatgctcta tcaaggcaag atagtggtgg atgtcaaggg agaagagaaa
    20521 aagcatctga cagttgaaga ccttatgcat ctcttccaga aaaatagtgg ccaaagtctg
    20581 gtcagtgatg aattagtttt gggataaaga aaaaggctga gatctggtgt ctcagtcttt
    20641 tatttcttgc gttttgcaaa atctacaaat ctaaatttct ctaacttatg gcgactttcg
    20701 gtaaactgaa attggcgtcc gtcttggaga tagacttttg atttaatcga aacgacataa
    20761 gggtctttgc caatgtccat gagaatcttg tctcggtcgc ttgagtggtc aatggtgatt
    20821 tccttctgag cataatcaat aaggagtttg aggccatttt ctatataaga atagatagac
    20881 tgctcagcaa tttggcgagt gagatttgga attagttcca tatccagata gtccgtatcc
    20941 agaacggata ccagatcatc caccacacgc tggcggacca ccttccaaac catccgaaac
    21001 tctgggaaac cggttatcag tgaggatttt ttatcaataa taatcttgtc cagactgacc
    21061 acgttggttt tagagcgcag tccaagttct ttaactagtt cttggtagct ggttaggttg
    21121 gatacaggga aattgacggt ttcttctttg acgacttgag aaccttgctc tcttatcttt
    21181 ttgatcaatc cttcctcttg gaggagagac agggcctttc ggacggtatc acgactcact
    21241 tgatattgct ccataaggtc gtgctcgcta ggaaggaaat ctccgacagc gtaagtctcg
    21301 ttttgaatgg tttcttggat ttgcttaaat aattgttggt atttcttcat tttgcctgct
    21361 ttaataggtt tttagtctag tttaatgaac acctgtatct attttaccat acaagtgcta
    21421 gaaaatttac aaaaaaacac cctttttata ccaaacaagt tgcagacaag tttggtatcg
    21481 tttacaatat acttatcaaa tcaaacttgc tttgacaagt ataaggagaa tcaaatggga
    21541 aaatttgaac aagaagccaa agatctgctt caggcaattg gaggcaaaga aaatgtgact
    21601 gccgtaactc actgtgcgac acggatgcgc tttgttttag gagatgataa gaaggctaat
    21661 gttaaagcta tcgagtcaat tccagctgtt aaaggaactt ttacaaatgc aggtcaattt
    21721 caggtaatca ttggaaatga cgtgcccatc ttttataatg attttacagc cgtttcaggt
    21781 attgagggtg tttccaaaga agcagccaag tctgcagcta agagtaatca aaacgtggtc
    21841 caacgtgtta tgaccactct ggcggagatt tttactccga ttattccagc cttgatagtc
    21901 ggaggattga tcctcggttt ccgtaatgtc ttggaaggtg tccattggtc gatgttggat
    21961 ggcaagacca tcacagaatc ctctcagttt tgggcaggtg tcaatcactt cctctggttg
    22021 cctggtgaag ctatcttcca gttcttacca gtagggatta cttggtctgt ttctcgtaag
    22081 atgggaacca gccaaatttt gggaattgtt ctcggaatct gtttggtatc gcctcagttg
    22141 ctcaatgcct atgcggttgc ttcaacgcca gcagctgata tcgcggcaaa ctgggtttgg
    22201 aattttggct attttactgt taatcgtatc ggttaccaag cccaagttat cccagccttg
    22261 cttgcaggtt tgagtctgtc ttatcttgaa atcttctggc gcaagcatat cccagaagtc
    22321 atttctatga tttttgtacc tttcttgtca ttgattccag ccttgatttt ggctcatact
    22381 gttttgggac caatcggttg gacaattgga caaggacttt catcagttgt cttggcaggt
    22441 ttaactggtc cagttaaagg gctcttcggt gcaatttttg gtgccctcta cgctccattt
    22501 gtcatcacag gtctgcacca tatgaccaat gccattgata cacaattgat tgcggatgct
    22561 ggtggcactg ccctatggcc aatgattgct ctttctaata ttgctcaagg ctcagccgtg
    22621 tttgcctatt atttcatgca tcgccatgat gagcgtgagg ctcaggtttc acttcctgca
    22681 accatttcag cctatctcgg tgttacagaa ccagctcttt ttggggttaa cgtaaaatat
    22741 atttatccat ttgttgctgg gatgactggt tcagcccttg caggcatgtt atccgttact
    22801 tttaatgtaa ctgcggcttc tattggtatc ggtggtttgc caggtattct ctctattcaa
    22861 cctcaataca tgctgccatt tgcaggaact atgctagttg cgattgttgt tccaatgctc
    22921 ttgactttct tcttccgcaa ggctggtctc tttacaaaaa cagagggcga tacgaacttg
    22981 caggcagaat tcgttgctca agaagaagca gaatttgtga accatgaacc agtagaactt
    23041 acttcggtag aaattatcag cccactaact ggccaagtga aagaattgag tcaagcgacg
    23101 gatcctattt ttgcatcagg tgtcatgggg caaggtctgg tcattgaacc aagccaaggt
    23161 gagttgacct ctccagttaa tgggacagtg acggttcttt tccctaccaa gcatgccatc
    23221 ggcattgtct ctgacgaggg agttgaattg ctcatccaca tcggtatgga tacagtaggt
    23281 cttgatggca aaggttttga aagtcttgta gtccaaggag atcacgttac agttggtcag
    23341 caactgattc gttttgatat ggatgtcatt aaggctgcag gtctggtgac agaaactcct
    23401 gttatcatca ccaaccaaga tgcttataca gcgactattc ccggaactta tccgacaacg
    23461 attcaagctg gagcatctct catggtcgct acacgaatct aatactcttc gaaaatctct
    23521 tcaaaccacg tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ctgtggctag
    23581 cttcctagtt tgctctttga ttttcattga gtataattga aatagcacaa atagggacgg
    23641 aagactgctt cctccctttt atcagaaaag gagaaacaga tgacacttga taaaggaaaa
    23701 gtagtctatc aaatctatcc aaaatcttac aaagacacca ctgaaaacgg tattggggat
    23761 ttccgtggga ttatcgaaaa aattccctat ttagccaaac taggcgtgga tatggtctgg
    23821 ctcaatccat tctatccaag ccctcaacgg gataatggtt acgatatttc agattatatg
    23881 gcagtggatc ctctttttgg tgatatggct gattttgagg aaatggtgtg tgtcggtaaa
    23941 gagcacaaga ttgactttat gctggacatg gtacttaatc attgttcgac agagcatgaa
    24001 tggtttcaga aagccctagc tggtgataag tattatcaag actttttctt catccaagac
    24061 caaccaacag attggcagtc taagtttggt ggttctgcat gggcgccttt cggggatact
    24121 gggaaatact atcttcacct atttgatgag acccaggctg accttaactg gcgcaattcc
    24181 aatgtccgta aggagctttt caaggttgtt aatttctggc gcgacaaggg tgtcaaaggt
    24241 ttccgatttg atgtgatcaa tttgatcggt aaggacgagg tttcagtgga ctgtcctgaa
    24301 aatgaaggga agccagctta tacagacaaa cccatcgttc ataactattt gcgtatgatg
    24361 aaccaagcca cttttggatc tgacgatagc tttatgacag ttggggaaat gtcttctacc
    24421 accatggaaa actgtgtcct ctattcgtcg cctgatcgtc aggaattatc catgaccttt
    24481 aattttcatc acctcaaggt ggactataag gatggacaaa agtggacctt ggctcccttt
    24541 gattttgaag agttgaagag tctttaccat agttggggta aggaaatgag tgataaagac
    24601 ggttggagtg ccctcttttg gaacaatcac gatcaaccac gtgccttgaa ccgttttgtc
    24661 gatattcaaa acttccgcaa ggaaggagcc accatgctag cagccagcat tcacctgtca
    24721 cgtgggacac cttatatcta catgggtgag gaaatcggga tgattgaccc agactatgat
    24781 tctatggctg attatgtgga tgtcgaatcg cttaatgcct atcagatgct cttagaagaa
    24841 ggaaagagtc agcaagaagc cttccagatt attcaagcta agtcgcgtga taattcacgt
    24901 attcccatgc agtgggatac ttctgaaaat gcaggatttt caacaggcac tccatggttg
    24961 aaagtcggta aatcctataa agatatcaat gtagaaaatg aaatcaaagg tctgattttc
    25021 accttctatc aagacttgat tcgactgcgt aaggaaatgc caatcatttc agaaggaaat
    25081 tacaaaccag cctttgaaga tagcaaacaa gtctacgctt ttgaacgcca gtttgaggat
    25141 caaaagttac tagtgctcaa taacttttat gccaaagaag tggaaatcga cttgccagca
    25201 gtctaccaaa atggacagat tttgatttca aactatgaag atgcagaagt gtctgagaaa
    25261 attctactca aaccctatca aacactggct atttatgtaa attaatactc ttcgaaaatc
    25321 tcttcaaacc gtgtcagttt tatctgtaac ctcaaagcgg tgctttgagc aacctgtggc
    25381 tagcttccta gtttgctctt tgattttcat tgagtataaa cttagcaagg agacctcttt
    25441 ttcagaaaag cagggttctt tttgctgtct attccatcct tgaaagtgta gaacaaactg
    25501 gtcagggtgg ctagtttgtg gtataatgaa agggattcaa aaagaaacag gagaaaaatg
    25561 atggatttac ttttagcaat tgtattgatt gtgctagctt ttctaggagg agctcttgga
    25621 ggaatgtact tggttcgtaa gcaaatcgaa aaagaatttg ctgacaaccc acgtttgaat
    25681 gctgaagcag ttcgtactct tttgagtgca aatggtcaaa aaccaagcga agctaaggta
    25741 caacaagttt accaccaaat catccgccaa caaaaggcag cccttgctaa caataaaaag
    25801 aaaaaataaa taaggaaaag tctgggatga aagttccaga cttctcacta tgttggttat
    25861 ggtttaggga tgagactttt tccttgcatt ctattgatat ttgattatga ttaagagaga
    25921 ggtagttgac attctatcaa gctgtcggtc agttcgttca gtacaaggaa tcataatgaa
    25981 cgatcaatca gaaaaaagac tagaaagaag actgtatgga taatcgacca attggttttt
    26041 tggattcggg tgtcgggggc ttgaccgttg tgcgcgagct catgcgccag cttccccatg
    26101 aagaaatcgt ctatattgga gattcggcgc gggcgcccta tggcccccgt cctgctgagc
    26161 aaattcgtga atatacttgg cagctggtca actttctctt gaccaaggat gtcaaaatga
    26221 ttgtcattgc ttgtaacact gcgactgcgg tcgtctggga agaaatcaag gctcaactag
    26281 atattcctgt cttgggtgta attttgccag gagcttcggc agccatcaag tccagtcaag
    26341 gtgggaaaat cggagtgatt ggaacgccca tgacggtaca atcagacata taccgtcaga
    26401 aaatccatga tctggatccc gacttacagg tggagagctt ggcctgtccc aagtttgctc
    26461 ccttggttga gtcaggtgcc ctgtcaacca gtgttaccaa gaaggtggtc tatgaaaccc
    26521 tgcgtccctt ggttggaaag gtggatagcc tgattttggg ctgtactcat tatccactcc
    26581 ttcgccctat tatccaaaat gtgatggggc caaaggttca gctcatcgat agtggggcag
    26641 agtgcgtacg ggatatctca gtcttactca attattttga aatcaatcgt ggtcgcgatg
    26701 ctggaccact ccatcaccgt ttttacacaa cagccagtag ccaaagtttt gcacaaattg
    26761 gtgaagaatg gctggaaaaa gagattcatg tggagcatgt agaattatga caaataaaat
    26821 ttatgaatat aaggatgacc aggactggta tgttgggtct tatagtattt ttggtggcgt
    26881 taacagtttg agcgactata agacagattt tcctctgttt gaattctcca aaatatttgg
    26941 agatgaagag tatggtttcc cgctttcagt tactgtttta cgctatggtt ctacctaccg
    27001 tttgttctcc tttgtggtag acatgcttaa tcaagaaatg ggacgaaact tggaagttat
    27061 tcaacgtcat ggggccctgc tcttggttga aaatgggcaa ctcttgtatg tagaattgcc
    27121 taaagaaggg gtcaatgttc atgatttctt tgagacaagc aaggtcagag aaaccttgtt
    27181 gattgcgact cgtaacgaag gtaaaaccaa ggaattccga gctatctttg ataagttagg
    27241 ctacgatgtg gaaaatctta atgactaccc tgacctgcct gaagtagcag aaacaggtat
    27301 gacctttgaa gaaaatgccc gccttaaggc agaaaccatt tctcaattaa cgggcaagat
    27361 ggttttggca gatgattctg gtctcaaagt cgatgtcctt ggtggcttac caggcgtctg
    27421 gtcagctcgt ttcgcaggtg tgggagcaac tgaccgtgaa aataatgcca aactcttgca
    27481 cgaattggcc atggtctttg aactcaagga ccgctcggct cagttccaca caaccctagt
    27541 cgtagccagc ccaaataagg aaagtttagt tgttgaagca gactggtcag gttatattaa
    27601 ctttgaacct aagggtgaaa atggctttgg ctatgatccc ctcttccttg taggagagac
    27661 aggtgagtca tcagctgaat taaccctaga agaaaaaaat agtcaatctc accgtgcctt
    27721 agccgttaag aaacttttgg aggtatttcc atcatggcaa agcaaaccat cattgtaatg
    27781 agcgattccc atggcgatag cttgattgtg gaagaagtcc gtgatcgcta tgtgggcaaa
    27841 gtcgatgccg tttttcataa cggcgattct gaactacgtc cggattctcc actttgggag
    27901 ggcatccgcg ttgttaaagg gaacatggac ttctacgccg gctacccaga acgtctggtg
    27961 actgagcttg gttcgaccaa gattatccaa actcatggtc acttgtttga catcaatttc
    28021 aactttcaaa agttggacta ctgggctcag gaggaagagg ccgctatctg cctctatggt
    28081 cacttgcatg tgccaagtgc ttggttggaa ggcaagatcc tctttctaaa tccaggttct
    28141 atcagtcaac cacgaggtac catcagagaa tgtctctatg ctcgtgtgga gattgatgat
    28201 agttacttca aagtggactt tttgacacga gatcacgagg tgtatccagg tttgtccaag
    28261 gagtttagcc gatgattgcc aaggagtttg agactttctt gttggggcag gaggaaactt
    28321 ttttgacccc tgctaaaaat ctagctgtgt tgattgatac ccacaatgcg gatcatgcga
    28381 ccctcttgct cagtcagatg acctataccc gtgttcccgt tgtgacagat gaaaaacagt
    28441 ttgttgggac gattggactc agagatatta tggcttatca gatggagcat gacttgagcc
    28501 aagaaatcat ggcggatacg gatatcgttc atatgacaaa aacggacgta gcggttgttt
    28561 cgcctgattt caccattacg gaggtcttgc acaagctagt agatgagtcc ttcttaccgg
    28621 tcgtggatgc agagggtatt ttccaaggga ttattacgcg caagtccatc ctcaaggccg
    28681 ttaatgccct cttgcatgac tttagtaagg aatatgagat tcgatgccaa tgagagacag
    28741 gatttcagcc tttttagagg aaaagcaggg cttgtctgtc aattccaagc agtcctataa
    28801 gtatgatttg gagcaatttt tagacatggt aggtgagcgg atttctgaga ccagtctcaa
    28861 gatttaccaa gcccagctag ccaatctaaa aatcagcgcc cagaagcgaa agatttcggc
    28921 ctgtaaccaa tttctatact ttctctatca aaaaggagag gtggacagct tttaccgctt
    28981 ggaattagcc aaacaagctg aaaagaagac ggaaaagcca gagattctat acctagactc
    29041 tttttggcag gaaagcgacc atccagaggg ccgcttgcta gcgctcttaa tcctagaaat
    29101 ggggctcttg cccagtgaga ttttagccat caaggttgcg gacatcaatc tggattttca
    29161 ggtgctgcga atcagcaagg cttcccaaca gaggattgtc accattccca cggccttgct
    29221 ttcagaattg gaacccttga tggggcagac ctatcttttt gaaagaggag agaaacccta
    29281 ttctcgtcag tgggcctttc gtcagttaga atcttttgtc aaggagaaag gttttccatc
    29341 cttatcagct caagtcttac gtgaacagtt tattctaaga caaatagaaa acaaggtcga
    29401 tttgtacgaa attgcaaaaa aattaggatt aaaaacagtc ctgaccttag aaaaatatag
    29461 ataatggata ttaaattaaa agattttgaa ggacccctgg acttgctctt gcatctggtt
    29521 tctaagtacc agatggatat ctacgatgtg cccattacgg aagtcatcga acagtatcta
    29581 gcctatgtct caaccctgca ggccatgcgt ctggaagtga cgggtgagta catggtcatg
    29641 gctagtcagc tcatgctgat taagagtcgt aaactccttc cgaaggtagc agaagtgaca
    29701 gacttggggg atgacctgga gcaggacctc ctctctcaaa tcgaagaata tcgcaagttc
    29761 aagctcttgg gtgagcactt ggaagccaag caccaagaac gggcccagta ttattccaaa
    29821 gcgccgacag agttgattta cgaagatgcg gagcttgtgc atgacaagac gaccattgac
    29881 ctctttttgg ctttttcaaa tatcctagcc aagaaaaaag aggagtttgc acaaaatcac
    29941 acgacgatct tgcgggatga gtataagatt gaggacatga tgattatcgt gaaagagtcc
    30001 ttgattggac gagatcaatt gcgcttgcag gatttgttca aggaagccca gaatgtccaa
    30061 gaggtcatca ccctcttttt ggcaacccta gagttaatca aaacccagga gctgatcctc
    30121 gtgcaagagg agagttttgg agatatctat ctcatggaaa agaaggaaga aagtcaagtg
    30181 cctcaaagct agacttgata gagaggaaag atgagtactt tagcaaaaat agaagcgctc
    30241 ttgtttgtag cgggtgaaga tgggattcgg gtccgccagt tagctgaact cctctctctg
    30301 ccaccgacag gcatccagca aagtttagga aaattagccc agaagtatga aaaggaccca
    30361 gattccagtt tggctttgat tgagacaagt ggtgcttata gattggtgac caagcctcaa
    30421 tttgcagaga ttttgaagga atactctaag gcgcctatca accagagctt gtctcgggct
    30481 gcccttgaga ccttgtccat tattgcctac aaacagccga ttacgcggat agaaattgat
    30541 gccatccgtg gagttaactc gagtggagcc ttggcaaagt tgcaggcttt tgacctgata
    30601 aaggaagacg ggaaaaagga agtattgggg cgccccaacc tctatgtgac tacggattat
    30661 ttcctagatt acatggggat aaaccattta gaagaattac cagtgattga tgagcttgag
    30721 attcaagccc aagaaagcca attatttggt gaaaggatag aagaagatga gaatcaataa
    30781 gtatattgcc cacgcaggtg tggccagtag gagaaaagca gaagagctga ttaagcaagg
    30841 cttggtgacg gttaacggcc aagtggtgcg tgaactagca accactatca agtcaggcga
    30901 caaggtcgaa gttgaaggtc aacctatcta caacgaagaa aaggtctact atctgcttaa
    30961 caaaccacgc ggtgtgattt ccagtgtgac agatgataag ggtcgcaaga cggttgtcga
    31021 cctcttgccc aatgtcaaag agcgtattta ccctgtgggt cgtttggact gggatacatc
    31081 aggtgtcttg attttgacca atgatgggga ctttacagac gagatgattc accctcgtaa
    31141 tgagattgac aaggtttatg tcgcgcgtgt taaaggtgtg gccaataagg acaatctccg
    31201 ccccttgacc cgtggtcttg agattgatgg taagaaaacc aagccagctg tttatgaaat
    31261 tctcaaagtg gacccagtca aaaatcgctc tgtggtgcag ttgaccatcc atgaagggcg
    31321 taaccatcag gttaaaaaga tgtttgaagc tgttggtctc caagtagata agttgtctcg
    31381 gactcgtttc ggacacctag acttgacagg actccgtcca ggagaatccc gtcgtcttaa
    31441 taaaaaagaa atcagccaac tacacaccat ggctgtaact aagaaataat gaaacgaatt
    31501 ttaatagcgc ctgtgcgctt ttaccaacgt tttatctcac cagtctttcc accctcttgt
    31561 cgctttgagc tgacttgttc caactacatg attcaggcta ttgaaaaaca tggttttaag
    31621 ggggtattga tgggcttggc tcggatttta cgttgtcatc cctggtcgaa aacaggtaag
    31681 gaccccgttc cagaccactt ttcccttaaa cgaaatcaag aaggggaatg aggtggggta
    31741 aatagatttc aaaatgataa aaacgcatcc tatcaggttt gagtaaactt gataggatgc
    31801 gttttagaat gtcaaaattt tatactcttc gaaaatctct tcaaaccgcg tcagcttt
//
LOCUS       DBERYS010000023        29608 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.023, whole genome shotgun sequence.
ACCESSION   DBERYS010000023 DBERYS010000000
VERSION     DBERYS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 29608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.023"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1504
                     /gene="phtD"
                     /locus_tag="ACL8LA_001169"
     CDS             <1..1504
                     /gene="phtD"
                     /locus_tag="ACL8LA_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HGS1812932.1"
                     /translation="DSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHP
                     ERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWI
                     KKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRM
                     PYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHP
                     NERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHP
                     ESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEA
                     LLEKVTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
     gene            1921..2301
                     /gene="dhaM"
                     /locus_tag="ACL8LA_001170"
                     /pseudo
     CDS             1921..2301
                     /gene="dhaM"
                     /locus_tag="ACL8LA_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
     gene            2759..3472
                     /locus_tag="ACL8LA_001171"
     CDS             2759..3472
                     /locus_tag="ACL8LA_001171"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGS1812933.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            3530..3934
                     /locus_tag="ACL8LA_001172"
     CDS             3530..3934
                     /locus_tag="ACL8LA_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812934.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQ"
     gene            4270..4923
                     /locus_tag="ACL8LA_001173"
     CDS             4270..4923
                     /locus_tag="ACL8LA_001173"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGS1812935.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            4933..5397
                     /locus_tag="ACL8LA_001174"
     CDS             4933..5397
                     /locus_tag="ACL8LA_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGS1812936.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            5410..6678
                     /locus_tag="ACL8LA_001175"
     CDS             5410..6678
                     /locus_tag="ACL8LA_001175"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGS1812937.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKFNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            6692..6952
                     /locus_tag="ACL8LA_001176"
     CDS             6692..6952
                     /locus_tag="ACL8LA_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812938.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            6963..8324
                     /gene="pdrM"
                     /locus_tag="ACL8LA_001177"
     CDS             6963..8324
                     /gene="pdrM"
                     /locus_tag="ACL8LA_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGS1812939.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            8478..9446
                     /locus_tag="ACL8LA_001178"
     CDS             8478..9446
                     /locus_tag="ACL8LA_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGS1812940.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            9462..10454
                     /locus_tag="ACL8LA_001179"
     CDS             9462..10454
                     /locus_tag="ACL8LA_001179"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGS1812941.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            10672..11715
                     /locus_tag="ACL8LA_001180"
     CDS             10672..11715
                     /locus_tag="ACL8LA_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGS1812942.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            11761..13464
                     /gene="lpdA"
                     /locus_tag="ACL8LA_001181"
     CDS             11761..13464
                     /gene="lpdA"
                     /locus_tag="ACL8LA_001181"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGS1812943.1"
                     /translation="MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTS
                     NDDDKSDDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            13528..14517
                     /locus_tag="ACL8LA_001182"
     CDS             13528..14517
                     /locus_tag="ACL8LA_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGS1812944.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(14590..15660)
                     /gene="xerS"
                     /locus_tag="ACL8LA_001183"
     CDS             complement(14590..15660)
                     /gene="xerS"
                     /locus_tag="ACL8LA_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGS1812945.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(16244..17794)
                     /locus_tag="ACL8LA_001184"
     CDS             complement(16244..17794)
                     /locus_tag="ACL8LA_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGS1812946.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(17810..18589)
                     /locus_tag="ACL8LA_001185"
     CDS             complement(17810..18589)
                     /locus_tag="ACL8LA_001185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGS1812947.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(18576..19427)
                     /gene="ylqF"
                     /locus_tag="ACL8LA_001186"
     CDS             complement(18576..19427)
                     /gene="ylqF"
                     /locus_tag="ACL8LA_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGS1812948.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(19681..24894)
                     /gene="zmpD"
                     /locus_tag="ACL8LA_001187"
     CDS             complement(19681..24894)
                     /gene="zmpD"
                     /locus_tag="ACL8LA_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease D"
                     /protein_id="HGS1812949.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            complement(24938..>29608)
                     /locus_tag="ACL8LA_001188"
     CDS             complement(24938..>29608)
                     /locus_tag="ACL8LA_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1812950.1"
                     /translation="IVEPEKVEALKEYTGKIEQPSAEDTKPNNENTNTPEEMSIQKKS
                     SALINMNFITDSSKVTGVGSATFIAPNVLLTVAHNFINNSTDNTTGEFRGDKSKNVYE
                     WVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVIKLPETTGREHVELVKNYSKVNLN
                     DKLNVHGYPARKYTHLKDATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNANGEVIGV
                     HQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDEKEVIKK
                     LELRNISSVELYSKDGNKYRHVTSLASLPSNAENYFMKVKSENFKDVMLPVTSITNDT
                     KDNRDVYKIVASANSLIQHENNNVLENYTYYLPKTQQSETGVYTSFKNLVDAMNSNPN
                     GTFRLGATMDAREVELPDGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGAT
                     VEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTIS
                     NSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIV
                     GAVENSALISNSYAEGNLNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTN
                     GNAIAGYNFNSIKANGTYSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKKA
                     TFVSKNTIKTEDFNFSSRYVTDYKNLENADSSKEKVYKNIEKLLPFYNRETIVKYGNL
                     VETSSNLYNKELLSVVPMKDKEVISDINKNKSSINKLLLYYADNTSETLNVNYQTDFS
                     NVAEYRIGGTNLIYTPNTLLRNYQNILDEVLPALNSVEYKSEAIRKVLDVSKDVSLTE
                     LYLEEQFNTTKTNLKDSLTKLLTADAAIAENNNKVIDNYVIEKIKNNKEALLLGLTYL
                     ERWYDFKYRDTKAKDLVMYHLDFFGKSNSSALDNVIELGKSGYNNLLAKNNVITYNVL
                     LAKNYKTNNLFDALEKYRKAFVPDKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGS
                     PYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGT
                     DLNDYVEKKAKEAAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDT
                     YQATFETNHPAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMT
                     HNSDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQV
                     ADPTQRFGSVDDLNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDP
                     DGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSP
                     IYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGK
                     QIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKEREDKFGKLNKVSFIDPNGSWARQQKV
                     TIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIF
                     ENKK"
BASE COUNT         8869 a         5596 c         5592 g         9551 t
ORIGIN      
        1 agattcaaga ccagaacaac caagtccaca atcgactccg gaacctagtc caagtctgca
       61 acctgcacca aatcctcaac cagctccaag caatccaatt gatgagaaat tggtcaaaga
      121 agctgttcga aaagtaggcg atggttatgt ctttgaggag aatggagttt ctcgttatat
      181 cccagccaag gatctttcag cagaaacagc agcaggcatt gatagcaaac tggccaagca
      241 ggaaagttta tcgcataagc taggagctaa gaaaactgac ctcccatcta gtgatagaga
      301 attttacaat aagacttatg acttactagc aagaattcac caagatttac ttgataataa
      361 aggtcgacaa gttgattttg aggctttgga taacctgttg gaacgactca aggatgtctc
      421 aagtgataaa gtcaagttag tggatgatat tcttgccttc ttagctccga ttcgtcatcc
      481 agaacgttta ggaaaaccaa atgcgcaaat tacctacact gatgatgaga ttcaagtagc
      541 caagttggca ggcaagtaca caacagaaga cggttatatc tttgatcctc gtgatataac
      601 cagtgatgag ggggatgcct atgtaactcc acatatgacc catagccact ggattaaaaa
      661 agatagtttg tctgaagctg agagagcggc agcccaggct tatgctaaag agaaaggttt
      721 gacccctcct tcgacagacc atcaggattc aggaaatact gaggcaaaag gagcagaagc
      781 tatctacaac cgcgtgaaag cagctaagaa ggtgccactt gatcgtatgc cttacaatct
      841 tcaatatact gtagaagtca aaaacggtag tttaatcata cctcattatg accattacca
      901 taacatcaaa tttgagtggt ttgacgaagg cctttatgag gcacctaagg ggtatactct
      961 tgaggatctt ttggcgactg tcaagtacta tgtcgaacat ccaaacgaac gtccgcattc
     1021 agataatggt tttggtaacg ctagcgacca tgttcaaaga aacaaaaatg gtcaagctga
     1081 taccaatcaa acggaaaaac caaacgagga gaaacctcag acagaaaaac ctgaggaaga
     1141 taaggaacat gatgaagtaa gtgagccaac tcaccctgaa tctgatgaaa aagagaatca
     1201 cgctggttta aatccttcag cagataatct ttataaacca agcactgata cggaagagac
     1261 agaggaagaa gctgaagata ctacagatga ggctgaaatt cctcaagtag agcattctgt
     1321 tattaacgct aagatagcag atgcggaggc cttgctagaa aaagtaacag attctagtat
     1381 tagacaaaat gctgtggaga cattgactgg tctaaaaagt agtcttcttc tcggaacgaa
     1441 agataataac actatttcag cagaagtaga tagtctcttg gctttattaa aggagagtaa
     1501 gtaaaggtag cagcattttc taactcctaa aaacaggata ggagaacggg aaaacgaaaa
     1561 atgagagcag aatgtgagtt ctagttctca tttttttcat gaaaatgtgc aaaatatagt
     1621 agattgaaac tagaatagta tacctctact tctaaaacat tgttagaaat cgatttgact
     1681 gtcctgttct tattttacta tatcttaaca gatagtgtaa ataaagataa actatttact
     1741 ggctaattaa tcagttaaac actagttaag gagtaatgat gaaaaaaaga acaatactat
     1801 tattgatgac cagtctgttg gctcttgtct taggagcata tggtttcttg gacatattga
     1861 tcctggatca ttctcatcag gattactctt tactgctatt ttagaaactg gggtggtttg
     1921 atggaaagta ttggtattgg tcttgttatc gtttcacatt ccaaacacat tgcagaaggt
     1981 gttgttgaac tgattagtaa agtagctaaa gatgttccga ttacttatgt aggaggaacc
     2041 gagggcggag gaattggaac gagttttgat taagtagata gggttgtttc cgaaaataca
     2101 gcagatactt tacttgcctt ttttgaccta ggttctgcta aaatgaactt aaaaatggtg
     2161 actgatttca gtgataaaag tatcatcatc aacagggttc caattgtaga aggtgcctat
     2221 aatgcagctg ctcttcttca ggctggtgca gaactgtcag ttattcaaac acagttagcg
     2281 gagcttgaaa tcaataaata aggaatttta ctataactct ttttataggt aagctattga
     2341 ttatctcaac tataaaatgt taagtaaatg atttcaaaat cagaaaggga ttgctttatg
     2401 cagttccttt ttattttaac aggagtgaaa ctatagtgtt tctaaattgt gaatcaatca
     2461 aaactgattg tgatggggct attctagctt tagaaacctt caaaaattaa aatttaaggc
     2521 aatcaattac ttggaagagt atgaaagcat ttagtttata ggaattctag gtctagaatt
     2581 acacatatat ttatgaatac ggggtgctcg atagttagta ttgttctatt ctgaaagatt
     2641 tgagctgtca gttgtataga aagtgttcga atttttttaa gtgattaaat tagttaattg
     2701 tatgaggtgc tttatgatat aatgttctta atgaattttc agaaaggaaa acctcaaatt
     2761 gttctacaaa tttctactct tcgacctcga ccacactctt cttgattttg atgctgctga
     2821 ggatgtggct ttgacccaac ttctaaaaga agaaggagtt gcggatattc aggcttataa
     2881 agattattac gttcctatga acaaggctct ctggaaagac ttggagctga agaaaatcag
     2941 taaacaagag ctggttaaca cgcgcttttc tcgtttattt tctcattttg gacaggaaaa
     3001 agacggtagt tttcttgccc agcgttacca attttatctc gctcagcagg gacaaacact
     3061 atcgggcgct catgatctct tggacagcct cattgagcgt gattatgact tgtatgctgc
     3121 gacaaatggc attactgcca ttcagacagg acgtttggct caatctggtc tagcacctta
     3181 tttcaatcaa gtctttatct cagaacagtt gcaaactcaa aagccggatg ctctttttta
     3241 tgaaaagatt ggccagcaaa ttgctggatt tagtaaagaa aagacgctga tgattggaga
     3301 ttctctaacc gccgacattc aaggtggcaa taatgcgggg attgacacta tctggtataa
     3361 tcctcatcac ctcgaaaatc acacacaagc ccagccgact tacgaagtct attcttacca
     3421 agacttgctg gattgtttag ataaaaatat tcttgaaaag atcacatttt aaaggagacg
     3481 agctaatgac tacaaaaaag ctaatattac tattgaagag tacattgaaa tgtctgaagt
     3541 tgattttaat gaagctgtta attatgaatt tacatctgac acttgtcaat tagcaaatag
     3601 tatttatcaa tctcttttta agttttttga taagaaaaat ttctctggcg atttaatttt
     3661 tacttggaaa tctccatcat tagtcaaaga aggggattat attgggagaa gggattcaca
     3721 agtagataat cttagagtaa taggaaatat atttccgaat tatcttacta atcgaaaata
     3781 tagcctcaat atgaatcgta atggctgtat gggagatttt cctcatgact tttttgatat
     3841 atacctagat catgtagcaa aatatgccta cgaacaaaaa gttaataata ttaaagagta
     3901 ttatccttta aaaagagcga ttttacacca atagaatgca ttgtattttc gatttttttc
     3961 taattttgac gactttttag aaaaaaatta tttaaagact atatggcaag tttctaaaga
     4021 aactcctttt tctgaaatgg attttaatat gtttaaaaat atttcagaaa agataatctt
     4081 cgaaagaggt agtaaaatgc ttaatgattt aaaaagtaat tataaaaaat agccagtctt
     4141 tataaactgt attcacgtat tcaaatcgta tatatagtcg atttattaaa tctttagata
     4201 aactgtaaaa agtggtttcc atagccactt tttgctataa tagaggcagt aaaaatgaag
     4261 gagtcggcta tggaacactc gtcttggcat gctttgatta aggcgcaatt acctgagggt
     4321 tatttcggga aaatcaatca gtttatggag caggtctatt ctcaggggat tatttatcca
     4381 cccaaggaaa aggtttttca ggctctcttg acaacactgc ttgaagaagt taaggtggta
     4441 attctagggc aagaccccta tcacggacca ggtcaagcgc agggcttgag tttttctgta
     4501 cctgactcta tcccagctcc accatccttg caaaatatct tgaaagaatt gtcagatgat
     4561 atcggagtta agaaatctca tgatttgaca gcttgggctg agcaaggagt cttgcttctt
     4621 aatgcttgtt tgactgttcc tgctggacag gccaatggtc atgctggtca aatctgggag
     4681 ccttttactg atgctgtgat tcaggtggtc aatcatctag atagaccagt cgtttttgta
     4741 ctctggggag cttatgcacg taagaagaag gcattagtta ccaatcctca tcacttgatt
     4801 atcgaatcag cccatccaag tcctttgtcg gtttatagag gattttgggg ttccaagccc
     4861 ttttccaagg ccaatgcatt cttaaaagag acaggacaag agccaatcga ttggcttaga
     4921 taaggagaaa atatgcctca gttagcgacg atttgctaca ttgataatgg gaaagaactg
     4981 ctcatgctcc atcgtaataa gaaacccaat gatgtccatg aagggaaatg gattggtgtg
     5041 ggtggtaagc tagagagagg agagacgccc caggaatgcg cggcgcgtga aatccttgaa
     5101 gaaacagggc tcaaagccaa gccagttcta aaaggtgtca tcacttttcc tgaatttaca
     5161 ccagatttag actggtacac ctatgttttt aaggtgacgg agtttgaggg cgacttgatt
     5221 gactgcaatg aggggacgct agaatgggtt ccctatgatg aggttttgag caagccgact
     5281 tgggaaggtg accacacctt tgttgagtgg cttttagagg ataaaccctt cttttcagcc
     5341 aagtttgttt atgatgggga taaattgttg gatacccaag ttgatttcta tgaataaagg
     5401 agaaagcaga tgctactaat caaaaatggt cgtgtaatgg atcccaagtc tggtttggat
     5461 caagtttgtg atgtcttagt tcaagatggg aaaattatca aaattgcgcc tgagatcacg
     5521 gaagaaggag cagaaacgat tgatgctact ggtcttgtgg ttgctcctgg tttggtcgat
     5581 attcatgtgc atttccgtga acctggtcaa acacataaag aagacattca tactggtgcc
     5641 ctggcagccg ctgcaggtgg ttttactact gtcgtcatga tggctaatac cagtccaacc
     5701 atttcagacg tggagacttt gcaagcagtt ctccagtcag ctgccaaaga gaagattaat
     5761 gtcaagacag ttgcgaccat tactaaaaac tttaatggta aaaacttgac tgactttaag
     5821 gcgctcttag aagctggtgc ggctggtttc tctgatgacg gtattccgct tgagagcagt
     5881 aagattgtca aggaagccat ggaggaagcc aaaaaattca atacctttat cagccttcat
     5941 gaggaagatc caggtttgaa cggtgttctt ggctttaatg aaaatattgc tagagaacat
     6001 ttccatatct gcggtgctac tggagtggct gagtacgcta tgatggcgcg tgatgtcatg
     6061 attgcctatg caactaaagc ccatgttcac atccagcatt tgtctaagga agaaagtgtt
     6121 aaagtagtgg agtttgctca ggggttaggt gcagaagtca cagcagaagt agcgccacag
     6181 catttctcta agacagaagc acttctttta acacaaggta gcaatgctaa gatgaatcca
     6241 ccgcttcgtt tggaatcaga ccgtcgtgcc gttatcgaag gtctcaaatc aggtgtcatc
     6301 acagttattg cgactgacca cgcgcctcat catgtagatg aaaaaaatgt tgaggatatt
     6361 accaaagcgc catctggtat gactggctta gaaacatccc tgtctctcag cttgacctat
     6421 ttagtagaag ctggtgagtt gagcttgatg gaattacttg aaaaaatgac atacaaccca
     6481 gccaagcttt acaactttga agcaggttac ttggctgaga atggtccagc agatatcact
     6541 atttttgatg ccaaggctga ccgctttgtg gactcccatt ttgcttccaa agcagctaat
     6601 tcaccattca tcggtgaaac cttaaaaggg caggttaaat ataccatctg taagggacaa
     6661 atcgtctatc aagcttgata ggagttagaa tatgaattat tttcgaaaac gtagggagag
     6721 acaagcgaaa tctaatagtg gaatttactg tcccgcagct aatcgttcca aaattcaata
     6781 tgaaactaaa gaaaaagctg accatgcggt tcaatatgat aacggaggtt tagttagaag
     6841 ctattattgt cgtacatgtg cgtgttggca tactacttca aaatctaata aacctccgct
     6901 aagtttgaaa aactatggtc tgaaactctt tggtttagat aaatcagaat agaaagagaa
     6961 gtatgtataa gacaaagtgt ttacgagaga agttagtatt atttttaaaa attttcttcc
     7021 caatcctgat ctaccaattt gccaattatt ctgcctcttt tgttgatact gcaatgacag
     7081 gtcaatacaa cactatggac ttggctggtg tatctatggc aaccagtatc tggaatcctt
     7141 tctttacatt tctaacaggg attgtgtcag ccttggtgcc tatcattggt caccatcttg
     7201 gtcgaggcaa aaaggaagaa gttgcgtctg atttttacca atttatttat ttggccttgg
     7261 gcctatctgt ggtcttgctg gggatggtac ttttcttggc accaataatc ttgaatcata
     7321 ttgggttaga agcagcagta gcggcagtag cggttcgcta tctttggttt ttatctatcg
     7381 ggattatccc cttgttgctc tttagcgtca ttcgttcctt gctggattcg ctgggcttga
     7441 ccaaactgtc catgtacctc atgcttttgt tactccctct caatagcgga tttaactatc
     7501 tcttgattta cggtgccttt ggtgttccag aactgggagg ggctggtgct ggtttaggaa
     7561 catccttggc ctactgggtc ttgcttggga tttctgttct ggttttattt aaacaggaga
     7621 agctcaaagc cttacacctt gagaaacgaa ttccacttaa tatggataac attaaggaag
     7681 gagttcgttt aggtctgcct attgggggaa ctgtcttcgc ggaagtggct atcttttcag
     7741 tggttggctt gattatggct aagttttcgt ccttgattat agctagtcac cagtcagcta
     7801 tgaacttttc aagtcttatg tacgcctttc ctatgagtat ctcatcggct atggctattg
     7861 tcgtttccta tgaagtggga gccaagcgat ttgatgatgc gaaaacctat attggtctag
     7921 gaagatggac tgctctcatt tttgcggcct tcaccttaac cttcctttac atttttaggg
     7981 gaaatgtggc cagtctttat ggtaacgacc caaaatttat cgatttgaca gtgcgttttt
     8041 taacttatag tcttttcttc cagttagcag atacctttgc ggcgccgctt cagggaattt
     8101 tgcgggggta taaggataca gttattcctt tttaccttgg tttgcttggt tattggggcg
     8161 tagcaatccc tgtggctacg ctatttgatt ccctaacaga ttttggagct tattcttact
     8221 ggatcggctt gattattagt ttgattgtga gcggggcgct ctaccgttgg cgtttaactg
     8281 tgattatgaa gagatttgaa tctttagcaa aatccaaatg ctaaaaagtt tgtgaaaaaa
     8341 tattcttgac aagaaaatcc cttgttttca cggggttttc ttttttattt tgtgaaattt
     8401 cttttagaaa aactttgaca gtatttgcca aaagcggtaa aatagtaagg taagaagaaa
     8461 gttagaaagg caagaagatg tcaactttag ataaaaatct tttgctagaa atgttccgta
     8521 agatggaaga aatccgtcgc atggacttaa aaattgcaca attagtaaag aaagggaaag
     8581 tgccaggaat gacgcacttt tctgttggtg aagaggcagc taacgtgggg gctatgttag
     8641 ctctcaatcc agatgatctg attacctcaa accaccgtgg acacgggcaa gctattgcta
     8701 aagggattga cctcaacgga atgatggctg aaatccttgg taaatacact ggaacctgta
     8761 aagggaaagg tggatctatg catatcgctg accttgatgc tgggaacctt ggtgccaatg
     8821 gtatcgtagg tggtggtatg gggatcgctg tcggtgcagc cctcagtcag caaatgcaaa
     8881 ataccggtaa aatcgttgtc tgcttctttg gagatggtgc gaccaatgaa ggtgttttcc
     8941 acgaagcagt gaacatggct tctatctgga acctgccagt cattttctat tgcattaaca
     9001 acggttacgg tatctctgcg gatatcaaga aaatgaccaa tatagaacat atccatcaac
     9061 gtagcgccgc ttatggaatt cctggaatgt tcatcgaaga cggtaacaat gtcatcgatg
     9121 tctatgaagg atttcagaaa gctgtggacc atgttcgcag tggcaatggt ccagtcttga
     9181 ttgaaagtgt aacttatcgc tggctcggtc actcatcatc tgaccctggt aaatatcgta
     9241 cgcgtgaaga agtggaattg tggaaacaaa aagatccaat cgaaaacctc cgcaattacc
     9301 ttattgaaaa taacattgca agtgccgaag aattggaaga aatccaagcg caagtaaagg
     9361 aagcagtaga agcttctgtt aaatttgcag aggaaagccc attcccaccg cttgaatcag
     9421 cctttgaaga tatttacgca gactaaggag aaagagataa aatggaaaca aaaacaatgt
     9481 ccttccgtga caccattatc cttgctatgt ctgaggaaat gcgtcgcgat gaaaatgtgt
     9541 tcttgatggg agaagacgtc ggtgtcttcg gaggagactt cggtacttct gttggaatgc
     9601 ttgaagaatt tggtccagaa cgtgtccgtg actgtccgat ttctgaagct gccatctctg
     9661 gagcagcagc aggagcagcc atgacaggac ttcgtccaat cgtcgatatg accttcatgg
     9721 acttctctgt tattgccatg gacaatatcg tcaaccaagc tgctaaaaca cgttacatgt
     9781 ttggtggtaa aggtcaggtt ccaatgactg ttcgttgtgc agctggtaac ggagttggct
     9841 ctgccgccca gcactcacaa tctctagagt cttggtttac tcacattcca ggacttaagg
     9901 ttgtggcacc tggaacacct gcggacatga aaggcctgct taagtcttct atccgtgata
     9961 acaactcagt tattatactt gagtacaagt cagaatttaa ccaaaaaggg gaagtgccag
    10021 ttgatccaga ctacacaatt ccacttgggg ttggtgaaat caaacgccaa ggaacggatg
    10081 taacagttgt tacttatgga aaaatgcttc gccgtgtggt tcaagctgct gaagaattag
    10141 cagaagaagg aatttcagtt gaaattgttg acccacgtac ccttgttccg cttgataagg
    10201 atatcatcat aaactcagtg aagaaaactg gtaaggttgt tctggtcaac gatgcccaca
    10261 aaacaagcgg ctatatcggt gaaatttcag ctattatttc agaatcagaa gcatttgact
    10321 atctagatgc accaatccgc cgttgtgcag gagaagatgt gccgatgcct tatgcacaaa
    10381 acctagaaaa tgcaatgatt ccaacagttg aaagtatcaa agatgcaatc cgaaaaactt
    10441 ataacaaaga atagggttac ataagataag ttatgtaaat aaaataaagc tgacgagaaa
    10501 ctttgtatct tttaggtgca gagctcttcg tcattaatat cttagattag agcacgggct
    10561 aaaagatcgg aaaaaagata aatctccttg gcttcatcga agccatcgtc gatttcctat
    10621 ttttcagtcg cttttgacgc cctttgtatc ataaatagaa ttggagaggt catggctgat
    10681 gacaagctaa gagcgactcc tgcagctaga aagttagcgg atgatttagg gataaacctc
    10741 tacgacgttt ctggctcagg tgcaaacggt cgtgtccaca aagaagacgt ggaaacttat
    10801 aaagacacaa acgtggttcg catttcgcca cttgcaaaac gaattgccct cgaacataac
    10861 attgcttggc aggaaatcca aggaaccggt catcgtggta aaatcatgaa gaaggatgtt
    10921 ttggccctgc ttcctgaaaa tatcgaaaac gatagcatca aatctcctgc tcagattgaa
    10981 aaagtggaag aagtccctga taacgtaaca ccatatggta aaatcgagcg tattccaatg
    11041 acaccaatgc gtaaggttat tgcccaacgc atggttgaat cttacttgac tgcgccaacc
    11101 ttcaccctca actatgaagt tgatatgact gaaatgttgg ctcttcgtaa gaaggttctt
    11161 gagccaatca tggaagcaac tgggaagaag actactgtaa cagaccttct ttcgcttgca
    11221 gttgttaaga ctcttatgaa acacccatac atcaacgctt cattgacaga agatggcaag
    11281 actattatca ctcacaacta tgtcaatctt gccatggcag ttgggatgga taatggattg
    11341 atgacacctg ttgtttacaa tgctgagaag atgagtcttt cagaactggt tgtagccttt
    11401 aaggatgtta ttggccgtac cttggatggt aaattggctc caagtgagtt acaaaattca
    11461 acattcacaa tcagtaattt gggaatgttt ggtgttcagt cctttggtcc gattattaac
    11521 caacccaact cagctatcct tggtgtcagt tcgacaatcg agaagccagt tgtcgtcaat
    11581 ggtgaaattg tgattcgccc aatcatgagt ttaggattaa ccattgacca ccgtgtcgta
    11641 gatggtatgg ctggtgctaa gtttatgaag gacttgaaag agttgattga aacgccaatc
    11701 tcaatgttaa tttaagagca agagtattca tttttaagat atagataaag gaaggaagac
    11761 atggccttag aagtaattat gccaaaagcc ggcgtggata tgacagaagg acaaatcgtc
    11821 caatggaata aaaaagtcgg agaatttgta aaagaaggag aaatcctttt ggaaatcatg
    11881 actgataaag tcagcatgga attggaagcc gaagaagatg ggtacttgat tgccattctc
    11941 aaaggagatg gtgaaactgt ccctgtaacg gaagttatcg gttaccttgg tgaagaaagg
    12001 gaaaacatcc caacagctgg agcagcatcg ccagaagcta gctctgtacc tgtagcaagt
    12061 acctcaaacg atgatgataa gagcgatgat gcttttgata tcgttgtgat tggtggaggt
    12121 cctgctggtt atgttgcagc cattaaagct gcccaattcg gcggtaaggt tgcccttgtt
    12181 gagaaatctg aacttggtgg aacctgctta aaccgtggat gtattccaac caagacctac
    12241 cttcataacg ctgaaattat tgaaaatatc ggtcatgctg caaatcgtgg tatcgtgatt
    12301 gaaaatccta acttcactgt tgatatggaa aaacttttag aaactaaatc taaagttgtt
    12361 aatactcttg ttggtggtgt tgcaggactt cttcgtagct acggagttac tgttcataaa
    12421 ggaattggta caatcactaa agacaagaac gtcttggtaa atggttctga attgcttgaa
    12481 accaagaaaa tcattcttgc tggtggttca aaagtcagca agatcaacgt ccctggtatg
    12541 gaatctccac ttgtcatgac tagtgatgac attcttgaaa tgaacgaagt gccagaaagc
    12601 cttgttatca tcggtggtgg agttgtcggt atcgaactcg gtcaggcctt catgacattt
    12661 ggttcaaaag tgactgttat cgaaatgatg gaccgtatag ttccagctat ggatgtggaa
    12721 gtttctaaga accttcgctt gattcttgag cgtaaaggaa tgaccatctt gactggtact
    12781 aaactgcaag aaatcattga ggaaaatggt caacttcgta tcaaggttga aggaaaagac
    12841 gatatcatcg caagcaaagc tcttctttca attggtcgta tgccagacct tgaaggtatt
    12901 ggagaggttg agtttgaatt ggatcgtggt tgtatcaagg tcaacgaata catggaaact
    12961 tcagttccag gcatttatgc accaggtgac atcaacggta ctaagatgtt ggctcacgca
    13021 gctttccgca tgggtgaagt ttccgctgaa aatgccctta aaggaaatca tgcagttgcc
    13081 aaattgaatt tgactcctgc agccatctac actctccctg aagtagcagc agtaggtttg
    13141 acagaagaac aagcccgtga gaaatacgat gttgccatcg gtaagtttaa ctttgctgct
    13201 aacggtcgtg ctattgcatc tgacgcagct caaggtttcg taaaagttat cgctgataag
    13261 aaatacggag aaatccttgg tgtacacatt attggtcctg cagccgcaga attaataaac
    13321 gaagcatcaa gcatcatcga aatggaaatc actgttgagg aaatgctgaa gaccatccac
    13381 gggcacccaa cttactctga agtgatgtac gaagcgtttg cagatgttct aggaatggcc
    13441 atccattcac ctaagaaaaa ataaaacgaa gatagattag actggaaggt tatttccagt
    13501 ttctatcata taaagtaaag ggatatcatg aaatacatta tcaatcattc aaatgacact
    13561 gcttttaata ttgccttgga agaatacgcc tttaaacacc ttttggatga ggatcaaatc
    13621 ttcctacttt ggattaacaa gccatctatc attgttggtc gtcaccagaa cactatcgaa
    13681 gaaatcaacc gtgattatgt tcgcgaaaat ggtattgagg tagttcgccg tattagtggt
    13741 ggtggagctg tttaccacga tttaaataac ctcaactaca cgatcatctc caaagaagat
    13801 gaaaataagg catttgactt caagagcttc tcaactccgg ttatcaatac cctggctcaa
    13861 cttggggtta aagctgagtt cacaggtcgt aatgaccttg agattgatgg taagaagttc
    13921 tgtggcaatg cccaagccta tatcaacggc cgtatcatgc accacggttg cttgctcttt
    13981 gacgttgatt tgtcagtcct cgcaaatgcc cttaaggttt caaaagataa atttgaatca
    14041 aaaggtgtga aatccgtccg tgcccgtgta accaatatta tcaatgaatt accaaaaaaa
    14101 atcacagtcg aaaaattccg tgatttactc ttggaataca tgaaaaaaga gtacccagag
    14161 atgactgaat acgtcttttc agaagaagaa ttggccgaaa tcaatcgcat caaggatact
    14221 aagtttggaa cttgggactg gaactatggt aaatcacctg aatttaacgt ccgtcgtgga
    14281 atcaaattca ctagtggtaa ggttgaagtc tttgctaacg ttactgaatc aaaaatccaa
    14341 gacatcaaga tttatggtga cttctttggt attgaagacg ttgctgcagt agaagatgtc
    14401 cttcgtgggg tgaaatacga acgcgaagat gttcttaagg cgctaaaaac cattgatatc
    14461 acacgctact tcgctggtat tagccgtgaa gaaatcgctg aagcggtagt tggataaatc
    14521 aaaaagaagt tggccaacta accaacttct ttttttgata tttatttaca taatttaaaa
    14581 tacgtaaaat cataaactat ccagagcatt cttttgttca tcattaacaa tatgggtata
    14641 gaggtcagtg acttgtgtgc tggcatgtcc tagttggtga ctgactaaaa cttgtgattt
    14701 agtagcatca tagagcctag ttgctagtgt atggcgcagt ttatggggtg ttacacgtac
    14761 tttaaaatcc tctgagtatt tagcaaccat tttctcaacg ctagaagcat cgatacgatt
    14821 aggaacacct ctgtagagag ttaaaaaaag ggctgtatct gttttttccg ttttatagcg
    14881 ttgattccga atggccagat aattctctaa ataaggttta gcaaaagcag cgacattgac
    14941 tgagtcacgt ttgccacctt ttcgagtaac atcaataacc atcattttta gattgagatc
    15001 tcttagatct agattaacag cttcagataa gcgaacacca gatgccaaga gaagggcaat
    15061 aatggctaaa tctcgttctt tatttttgtt gaatgatgag agagctcgat ttgaaagctg
    15121 ttgtgggtgc tcttgatcga tataagttag aaaaccttct gtttcatcac ctagaaagag
    15181 tttttgcttg atattttcag ctctggcagc aagggtttct ttctttttct tggttgaaac
    15241 ttttttcatt acattacgat agaaataagg ttccccctga tcgttttcaa cctcctcggt
    15301 tagatacttg taaagactag aaagtgctga taaggttcga ttgatagttg tctgtgaaac
    15361 accttgtttt gttgtattag cattcagcaa gggacgttca cgtagataaa ggataaagga
    15421 ttccatgtct ttcttagaca tattttccaa aactgataaa ggaatatcgg atattttatc
    15481 agcgtttgaa ataccagact ccaaaaccca gctgaaaaat cggtcatatt ccttaaggta
    15541 ttcgtacagg gttgtaaaac tgtagggcac agccagttta gattggtagt attccagaac
    15601 ataccagggc atgagttgtt ttagtttgtc gattcgttcc agtaaaatct cacgtttcat
    15661 gtattttctc cataattaag tatactagta gtatagcata gacttgtata tttttcaaga
    15721 aaattatagt ttttcagaaa gatgctatag gagtttttca aagtgataaa aagacttaga
    15781 cctacaaaca aaacagaata aggaaaataa taaaaatctc acccactaaa cttctgttaa
    15841 tactacataa ctgaagagct atataaggag ctatgagata caaaacacat tgtataatag
    15901 attgaaacta gaatagtaca tctggacttc taaaacattg ctagaaattg atttgattgt
    15961 cctgattgtt ttatcctatt tctatttcat ttctctatat aattgctatt gttttctttt
    16021 tcatgatttt aatcttactg tacagatttg atacagtaaa ctcctttcta gctttatact
    16081 ttatcatttc taacttaatt atagtcttat ttttaataaa gttcaattat ttatatgtaa
    16141 aattcctcat cagtaaaaaa taaccgatct catttctgag aatcggtttt ctaaataaat
    16201 caaactgatc atttacataa cataaattat gtaaatgatc atactacaac aacaaatctt
    16261 tgacttttcc aatttcactt tttggaataa ccaggtgaat catatcaccc agatacattc
    16321 tggttgagcc gttaactgtt tggctcttgc cattatggac ttgagttgtg atgaggacgt
    16381 tgtgtggtaa gttgagttca tgaacttgtt tcccagcaat tttatcagaa actggtattt
    16441 cgataagtgt aacttctcct tcgctagata cttcttctgg aagcattttt tccagcatgg
    16501 cttcatagac tggcgtacct ttgagcaaat ccatgataat ataagaaaca agagtgacaa
    16561 gaccaagtgg cataaggttg cgaatatctc ctaccatctc agttacgagg atcatagcgg
    16621 ttaagggagc ttttgatatg gctccaaaat agccactcat tcctagaatg acaaatatag
    16681 ggaattgctc ttgactgaca agtccaagat tgacacagat aacaccaact aaggcaccaa
    16741 gcaaagaacc aagagctaaa atggggagga aaattcctcc tggcagtcca cttccatagc
    16801 taatcatact ccaaataaag cggattaaaa agtaagctaa taaaacttgg aaactaaaat
    16861 tttgttcagt taaagaaagg acaagctgat ttccgccacc aatgatttga ggtaagaaga
    16921 ttccgactgg tatgataagg ataaaagcca agatgggata ataagcccta tccaaatgga
    16981 ttttttgacc aatcaagtca taaactcttc caacgtttaa tacagctttc tcatagagaa
    17041 aacctgaaaa tccaaggaaa attcccatga cgagatatat ccaatactga tctagggtca
    17101 tgggaggaat gttatctggc atatccaata ctggtgtcaa accgaacatg agtagagaca
    17161 caaagtttgc tacgatgctg gctgctagag ttgagaccca gaaaaagcgc gaaaaatggt
    17221 gatagacttc ttctacaaca aagagaagtg ctgcaatagg agcattaaag gctgcggcta
    17281 aacctgctgc agctccactg gcaatcaagg aacgttcctc tactggactg gatttgagcc
    17341 acttggcaat tcctttacca ccaactgctc caagttgaat actgggtccc tctcgaccca
    17401 gcatgagtcc actggcaata gcaagaatac ctagcacata ttttttccaa agaatgcccc
    17461 accagttgag ggacatgagg cctttcagtt cggcttcgac ttgaggaatt cctgagcctt
    17521 taatatcttt ttctgaccgt gttagtttgg cactgagcca acagatgagt atataaaaca
    17581 aaaccagtac aaaaagattg cgcactaagt acccttgatc ttgataaact ccttgtatca
    17641 ggtggaagcc cttttcaatt aagaaacgaa aggatccgac gataattcca acgatgaggc
    17701 cgacaatgat tcctcgacca acttgggata gtatagtgct ggatgcaaag gcgaattctt
    17761 tcttagaacg gactatttcc gactgttcct ccatggtatt tcctttcaat taactttctt
    17821 ttttacctaa aaccagtgat ttaacgggtt caaagctggt tcggtgaatt ggggtaactc
    17881 ctagttttgt gaggccttcc agatgtttag ctgtgccata tcctgcatta gtagcgaaat
    17941 catagccagg gaactgctga tcgtattcct tcatcaattc atcacgtgtt accttggcta
    18001 ctatagatgc tgctgcgata gagagggagt tggcatctcc tttgataatg gaggtttgtg
    18061 aaatgggcaa gtccagtttc atggcatcaa tcaaaaggtg ctctggttga gggctgagct
    18121 gggagattgc ttcttgcatg gctagtttgg ttgcttcata gatgttgact tggtcgatga
    18181 cctgattatc tatgatacca attccaatcg acaaggcttg gtcttgaacg gcttggaaaa
    18241 tctccagatg tttcttttta ggaattttct tgctgtcgtt gagaccttta atcttacaat
    18301 ttttaggtaa aatgacggcc gcagcgacta caggaccagc aagaggacca cgaccaacct
    18361 catcaatacc tacaattaag gtcaatcctt gcttataaag ttctttttca taagaaagca
    18421 tggattccaa gcgcaaattt tcatctaatt cagcttgaat ggctctttta cgcttgctga
    18481 tttccttttg aactcctgag cgattatcct tttcaagctc taaaaaaata gggctttcta
    18541 actccttgac tgtcacaagg aattctttga tttctttaat cgttgccatc gaggtcttcc
    18601 aatgtatcta aggtatagtt accgagtttg ccatcacgga cttccttcac gaagagactg
    18661 taaaaacggt catagtcatc acggaaaccg agggcgcggg tcatatccat aataatcaca
    18721 ggcgcttctt cttcaatttt catttgtttg aagcgttcag ccagcttttc tggataatgt
    18781 tctttgaaat aattgatacc aaaaatggta acctcatcca taggaagcaa ctgatccttg
    18841 atagctccag tcaatgccaa cttaagtgca acagtttcat cctcaaactt aggccagaga
    18901 atccccggtg tatccaagat ttccaggtct ttattggttt taagccattg ttgacctttt
    18961 gtgacccctg gcttgtttcc aacaacagca atctttttac cagccaaacg gttcatcaga
    19021 gttgatttac cagcgtttgg aatcccgata atcatagtac gcaaggtttc aatctgaatc
    19081 ccacgttctt tctggcgagc aatcttatca gccatgagct tcttggccgc atctgttaca
    19141 acttttacag tcacttgctc cttggagttg atagccagag tttggattcc ttgtgattca
    19201 aaatactgac gccattcctt ggtcattgct ggatcagcca aatccgcctt atttaaaatc
    19261 aagagttttg gtttatcacc aacaatcttg gtcaacatag gattttgact agatagaggt
    19321 aagcgtgcat ccaccaaaat cgttacaaaa tcaacaaatt ttaaattttc ctgcacctgt
    19381 cgacgcgctt tagacatgtg accaggaaac cattgaatag tagccattga gtttttttcc
    19441 tttcgtagca agggtttaga gatcctattt tattttacta tcgtttgaac ataaagggtt
    19501 ctcaaatgaa tgatgttcaa acagtttttt gttttatggc atagaaatct accttatatt
    19561 atatcatgcc ttctttattt tgtccgatga tagcctttca agtaccctat atagaaaaaa
    19621 gccatcaaat gatgactttt cagagggagc aaaaaactgt tccttctata ttctaaacat
    19681 ttaaatgtct tctttaaaca gtttattaaa gaaaccgtct gtgtttttaa gaagggcttt
    19741 gaagacctta gatttaagag caacagtgtg agagaagtca tctggtttct tcaaatcttc
    19801 ttcaactgct ttatcaaata aaggccttaa ctcatcatag ttactgatat ggacgccatc
    19861 aatatcgata gcctcaaaac ctttcttagc tttagcaaga acttccccgt accaatgtct
    19921 cttccactct tcaagagtat taaagtttcc tttcgatact ttattaatta ttagcttatc
    19981 gcttagtaaa ccgtggttgt ttttatctgc ttctgctttg tatttatttg atacataact
    20041 aataaagcca ttttcataac cataatagcc ccacatacgg aaagcattgt gcttaaatga
    20101 taatgaacca ggagcacctt gactcgtatt cccaccgtaa attcctgcca tcatattgac
    20161 atttacgtaa gctgagtttg gtgtaaaatc ttctggtcta taacgtccat tacctggatt
    20221 tccatgtttt gtcatgaagt tattatccac taaatcattg acgctagata aagtcattac
    20281 cttttcttca tctttcaaat cacggatact atcccattgg tttggaccta aaagatcatt
    20341 atactgaatc ttagagcgca tttctttgtc aatcttcttg aaccacttac tattgtctcc
    20401 tttaatttta ggaagtacag catcagcttc tacatgatca agcaacatca tagcttcatt
    20461 gtagtttctc atgtagcggt caatgtcatc acgtgtcttt aatttgtcag gatctgggtt
    20521 gtaccattgg ttaccatcat ttggacgatg gtaagccatg ttaattccaa gtgcgccgta
    20581 ttcaccatta gtcgttgagc tgtcaggtgt ttgcaacata ccttgagcat aggcttcaac
    20641 gtctgtacct tgacgatgct tatgaccacc gaagtaaagc attctatcgt ttacgtgtgt
    20701 tgtttcgtga gtaaaggctg aaataccaaa tgggctaatc atatccgtca ccataaacaa
    20761 gaccttgtca tcattatttg gattagggaa tattgatgcc atagcgccca tacgccagtc
    20821 tctatcatgg tgtcgccatg ttggaccata taattcacga attggagcta tagggttacc
    20881 tttactgtca aatcctcgac gacctacagg agaaccattg taggtctggt tatcccaaac
    20941 tggtgtcggt accatatttt gactcttaag aagtctatca cgaacattag gcagagctaa
    21001 tctggaccag aaatctaaat aagtttgttg agcatgtgca acttcatcaa tcttagtttt
    21061 gaactcatta cgagcttctt ctgtatgttt tccatatttc tcaaatgaac tgtacgccat
    21121 cgtgttatat gttgaaatta agaacatatg ggcatcttta agcgttagaa gtggtaaaat
    21181 catacgacca tgaacaccgt tatctaaacc atcgaacaca cgatgacgtt tatctttcaa
    21241 ctctgtattt gttgtcttag gttctgaaac ataaacattc ttagctgagt ggataaacca
    21301 atcattgatg tctttatcct ctgtaaacag acgcatgttg tattctaaga agttacgcaa
    21361 attactttta ccagtattac ctgcgatgat ttcacggtaa gcatcctgag tccgatcccc
    21421 tttcaagttc ttttcacttg aaccgatgtt gatgagtctg tcaataacac ttggtgtttt
    21481 accataaaaa tcaggtttga acatcatgat gttttttata cttatgtcac cgtatttgat
    21541 atcatagtaa cgattgagat aagtcagagc catcatgatt ttagctttat tggtttccac
    21601 tttcttgaga agtgatttta tcgcagcttc atcaccattc aattgatggt cttcattttc
    21661 gaccaaggat ttgactaact tatctagatt ttgcttcact tcggcaaagc tttcttctaa
    21721 gtatagatct ttaatataac cattcttacg ttcatccgta ttctctgcag gtttatcacg
    21781 tctactcatt aaatctctta cttcttgtga aatcaattcc acacttgata attgagattt
    21841 aacatcagca atcaatttat cacgattttt aacaaccata ttcggtgtat aaacaacatc
    21901 accaagacca gatacactat attcacgcac ttgttggact ttagaatcag tattcttagt
    21961 aacatccatt tcttccttag taccatcagc atagtgaacc ataatcttat caatatctga
    22021 taaatcggtc acgaattgac catccttcat ccccgtaaca gaaaggactt ctttcttagc
    22081 taagtttgaa tcttctgcaa gtttgttacc ttggttgaca atccattctt tattataaaa
    22141 tggttgaagt ttctcaatat tacgataagc taaatcacgt tcggatttgt agtcttgagt
    22201 tgacttatat tcatcttctc ttctggttaa acgatttaag cgattgacca caggttcgtt
    22261 gatttcgtat ttatcagcag taagtccaaa atttgcaatt ctcttattag cttcatcttg
    22321 actaatttct ttaattttgt cacgagaacg tttgtatgaa tgcttacctg aactgacacc
    22381 tttgacaaca aagttacgct ctaaccagcc atttgtgaaa taaccaccat cgtagtccaa
    22441 atcggaagaa ccaaagaact cttcaccatt ttcaactctc atcattgaaa tattatcttc
    22501 aagtttacca aaaggaagat tgctattgat aaatccacct gtttcaaaca aaactttagt
    22561 cttaatggtt cctttggtga ctgatttacg aattgaaccg tatttaccaa ttcccataga
    22621 gtcgttacca ttatctgttt tagcaactaa accacctata cgggctctgt tagcaacaat
    22681 atcagcctcg acatatgctt ttgcaagatt acctctccaa atctcaccgg caatgccagc
    22741 catatattga cctttatcac caacacctgt aagattaccg atgaaggcta catttgtcaa
    22801 ctgaccacca ctatcgattt tattgacgat acccgcaata ttgttattag caactacatt
    22861 accagttacc ttaacatctt caacagttgc atttttcacc atattggcaa caggtgctat
    22921 tccgtcagcc caaggcatat tgatatccac attacctaaa ttaatatttt tcacagaacc
    22981 attttcaaca cggtcaaata agggacgggc aatgttatga attgtatatt gtttaccatc
    23041 aacacttgat agatgtccct taaacgtacc tggaacatat tgtttgttag gtgttggtac
    23101 attcgttgca ttcaaatctg ctccgatttt aaatgtacca tttttatcag cattcatggc
    23161 atctactaac tctttaaagt tataataaac gtcatcaact tttggaactg gtttttcaat
    23221 ataatgaaca tacttgttac gaaccttcgt tgggtctgta tgttgaatca aatcttgagc
    23281 ttcggcagtg attttataaa gtttcttacc ttcttcagtc acttcttcaa ttttatcaac
    23341 tgctaaacgt gttactttat tatcacgact agtaattttt aagtacattt tcttcacatc
    23401 ttcatctgaa ggtttttcag tcataaaatc actaggtgtt tcagtaccat cgtcatttac
    23461 cttcactaaa ttagtagatg cgatattttt aatttcaact tttttcaaat ccaagcgaag
    23521 tggtttctct tctaatgttg cagtttcttc tcccttgcca cggtcatagg tcatagtggt
    23581 ctctatctta tagtccttgt agaattttaa atcttctacc tgcttatcta attgctctga
    23641 agtcaaatct actgttttga caatttcatt tccatcttta attgtcgcag taattttatt
    23701 gattgtgact ccatctgtat taactaaatg ataagaaaga gttgcggatc ggtttaatac
    23761 atttgtattt gttctatcta aggtcaaaac aggagccgtt ttttctttag gtttttcttt
    23821 ggtacctttt acaagaattt taggcttcat ttctttcaaa atagttactg tttcaactgg
    23881 gtcactgatt tttttgccat gtaactcttc atagctagta acaatttgac gttcgccatc
    23941 ctcaccaact tgtctaacag aatcatcgcc tataaatttg ctaggatcag attcttctat
    24001 agtggtttta ggaatagtct ctgtccgaat atcggtatgt aaagaaggca actcttctct
    24061 ttctgatgca acttctgaaa ctgtagatat tggttcagta tactctggaa tgtcgctaac
    24121 aggtggctca atcaagtttc cattttcatc cactcctgtt gtacctactg gctcagtata
    24181 ttctgggatg tcgttaacag gtggttcaat caagtttcca ttttcatcta ctcctgttgt
    24241 ccctactggt tcagtgtatg ctagtttctc atgaacttca ggctcaacaa ggtttgcacc
    24301 tattggttgc gtatagtcag gcttctcatg aacttccggt tcgcctttct ctgttacaac
    24361 tttgggttgc tcaactggcg aaacagttga tgctggagaa accgacgaaa cagttttatc
    24421 atcaagctcc aagtaaccaa catatctgta accagaaatt tgcaacacac catcacgtcc
    24481 agactcatgg atgttagcat tcaaattaag tgctgaagca gttgatagtg taactaaacc
    24541 tgtcgcccct acaatcaaaa atgtagctat ttttttattt ctattatctt ttgtgataat
    24601 cacaataaga ctcccgatag ctaacaaacc caatgcagtc ataatagatt gagaacttcc
    24661 tgtgtttggt agtgcgtctt tttcataatc caaagcatat gactctttgg attcatcagg
    24721 tctaccttgt ttgagttgat cacgttcagt ttgtgtcaaa ctactataat ctaagtaatg
    24781 ataagtcgat gcaccaacaa ctgacggtgc aaataaaaga ctccccaaga ataccgaacc
    24841 aacaataccc tttattttcc gaatggaaaa tttatctttt tttaataatg acaattctaa
    24901 tcctccacct tcacttttta tttcctaata gtaaacacta ttttttattc tcaaaaattg
    24961 aacttctaaa atcaccggtt tggtcaagat aagctttaaa gattgctttt ttcagtttaa
    25021 gaactctact atttgtctca ggatataatt tggcaacttc atcctcagca tcaaatttta
    25081 ctgcatcttc aatcattttt tcaagtctat taatattatc aatagtcact ttctgttgtc
    25141 gagcccaaga accgtttgga tcgataaaac taactttatt taactttcca aatttatctt
    25201 ctctttcttt atacatatct ttcttgaaat ctatccaaga attatactgg ttattaaata
    25261 cttttgaaag aacaatttca tcagtaacta aacctatttg tttaccatat gatgtaataa
    25321 cttttccttg agctttcgct tctttttcat actgattaga aatatatgga accataccgt
    25381 ctttgtaccc cttggctgcc aataactcaa atgcaatacg tcgtcccatt aaatctcctg
    25441 gtgttcctat tttactgctc aatgctgaat aaattggtga gaatagatta attgtatagt
    25501 agccattccg cttataagtt gcttcatcta cataacctct tcttgttata atatcatgtt
    25561 ctatcaaact gttaaatgta gttaatttag taagttcttc tggttttaat ctacgtacaa
    25621 cattcgttgc ataaacagaa tttccatcag gatcaggttt atattcattt tctatttttc
    25681 ttaataaatc atatttttga tttgtatcta atttagctac agctttacct tctagtattt
    25741 caagcatgta tattacatca aacatattat gcatatactt atttagatca tcaacacttc
    25801 caaatcgttg agttggatct gctacttgaa gccttgttga ttcttctgat tgatcatatt
    25861 ttaatatgga attaatagtg actgttggat cattaggatg atcaggagct tgtagcagtc
    25921 ctttagcata aaactctggt ccaagtccat ttcgtcttcc atagcctcca agataaattt
    25981 ctctatctga gttatgtgtc atttcgtgtg tataagttac cgcaccatct ttatcaagca
    26041 tgcgatatgc catataatag aatgcatccc ctgtagcata tgctccatta gaattatgaa
    26101 caacattatt tcccgctgga ccgaagaaat gttttatagc tggatgatta gtttcaaaag
    26161 ttgcttgata tgtatcttta tcttcttttt catctgtacc aaatctaaat gcatcataga
    26221 ctagaacact tctatataat ttttctttat ttttatcatc tagtattcta taccaataat
    26281 catagtgatc tctaaatcta acagccgctt ctttagcttt tttctcaaca tagtcattta
    26341 aatcagttcc tgctggatgt tctttacttc tatatctatc ataagcaccg aaacctatag
    26401 tcgaaatgtt ggctataata aacaccgatt tttcaggtaa tgttaatagc ggcaacacca
    26461 tacttggata tttccaagat gggctagtta atctatcata tactccaatt gaatatggac
    26521 taccagctat tgattgctta tcacttacct ctttaatagt agatttttct tctacaatat
    26581 atgctttagt ttgttcttta aaccactcat tatttgtttt atctggtaca aatgcttttc
    26641 tatatttctc taatgcatca aataaattat tagtcttata atttttcgct aataaaacat
    26701 tataagttat aacattattt tttgctagta agttattata tcccgattta cctagttcga
    26761 taacattatc taacgctgaa ctatttgatt taccgaagaa atccaagtgg tacatgacta
    26821 aatctttcgc ttttgtatca cgatatttga agtcgtacca acgctctaag tatgttaatc
    26881 ctagaagtaa agcttcttta ttatttttaa ttttctcgat tacataatta tctattacct
    26941 tattgttatt ttcagctatt gcagcatctg cagtaagaag ttttgtcaaa ctatctttca
    27001 aattagtttt tgtggtattg aattgttctt ctaaatataa ctccgttaaa ctaacatcct
    27061 tagaaacatc taatactttt ctaattgcct ctgatttata ttctacacta tttaatgctg
    27121 gtaatacttc atctaaaata ttttgataat tgcgaagtaa tgtattcggt gtataaatta
    27181 agtttgtacc gcctattcta tactctgcta catttgaaaa atcagtttga taatttacat
    27241 taagtgtctc tgatgtatta tccgcgtaat atagtaatag tttatttata cttgatttat
    27301 ttttgttaat atcactaatt acttctttat ctttcatagg gactactgac aataattctt
    27361 tattataaag attactactt gtctctacta aatttccgta cttaacaatt gtttctctat
    27421 tataaaacgg taatagtttt tcaatatttt tatatacttt ttcttttgat gaatcagcat
    27481 tctcaagatt tttatagtca gttacatatc tagaagagaa attaaaatct tctgttttaa
    27541 tagtattttt agatacaaaa gtagcttttt tctcttttat ttgtgcatct tctaatactt
    27601 ctcctctttg aacagtagaa tccttagtta agatttcatc atcttcttgt actacgttca
    27661 caaccttatt atttttatta ctgtatgtgc catttgcttt tatactatta aaattataac
    27721 ctgcaatagc attaccattc gttacattaa catcgcttag tacattagaa agtcgaccgg
    27781 atttttctaa tcctccaacc ggatcccaaa gattaccaac tactccacca acgtttgcaa
    27841 aacgttgaac attatttaga tttccttcag cataactatt actaataagc gcactatttt
    27901 ctactgcacc aactattcca ccaatagact ggtctccttg tgttgcattt gaagataaat
    27961 cgattgatgc gaacgactta tctattaatc ccctcggacc agaaagtttg cctactaaac
    28021 ctccgatttg ataactagca actgttttat atgtattagt tattctacct gtatagctac
    28081 tgttagaaat agtagaatta ttaacttgtg atactaatcc tcctatgcta cgttccccag
    28141 ctatcgctcc atcagcatga acgttatcaa tatgagtatt gttatttgct tctttcgcaa
    28201 gtgttgcagt atcgtccttt gcggaaatat taacatcttt tagtgaaagt ttttctactg
    28261 tagcaccatt taattcccca aataatggtt ttttcaaatt ataaatagca taatatttct
    28321 cattatttgt tcctactaat attccatgga atacattatt cacataactt tcttgaccat
    28381 ctggaagttc aacttcacga gcatccatag ttgctcctaa acggaatgtg ccattaggat
    28441 tgctattcat tgcatctact aaatttttaa atgatgtata taccccagtt tcactttgct
    28501 gagtttttgg taaatagtat gtataatttt ctagtacatt attattttca tgttgtatca
    28561 gactatttgc acttgctact atcttataaa catctctatt atctttagta tcattagtaa
    28621 tgcttgtaac tggtagcatc acatctttaa agttctctga tttaactttc ataaagtaat
    28681 tttctgcatt acttggtaaa gaagcaagtg aagttacatg tcgatactta ttaccatctt
    28741 ttgaatatag ttctacagaa gatatatttc taagttctaa tttcttaata acttcttttt
    28801 catctttagc gtcatctttt ttctcatctt tatgacgata aagttcatcg tatactggtg
    28861 gaatttcatt acctgctata atactcttaa tccaagctaa ttgtgtagga gataaaatca
    28921 atccgcctga gcgattttgt gctccatttt gatgcacacc aataacttca ccattagcat
    28981 taaatattcc tccaccactc ataccaggat ttccaccttg atattgaaca ccgtaggtgt
    29041 tattcgcata ttcttgttcc atttctaccg tagcatcttt taagtgcgta tatttcctag
    29101 ctggatatcc atgaacattt agtttgtcat taagatttac tttcgaataa tttttaacta
    29161 attctacatg ttctctgcca gttgtttcag gtagcttaat cactgctaaa tcatagataa
    29221 atccttttgg ataatctttt ttattataaa aatgaatatt gttagcagta aatgttcctt
    29281 tttgtccatc aggagtcacc cactcatata cgtttttaga tttatcacct ctaaattcgc
    29341 cagtagtatt atcagtagaa ttattaatga aattatgtgc tactgttaaa agtacatttg
    29401 gtgcaataaa agtagctgat cctactccag taaccttcga cgaatctgta ataaagttca
    29461 tattaattaa tgcactagat tttttctgaa tactcatttc ttctggtgta ttagtatttt
    29521 cattatttgg tttagtatct tctgcactag gttgttcgat tttaccagta tattctttca
    29581 gtgcttctac tttctctggc tcgactat
//
LOCUS       DBERYS010000024        28675 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.024, whole genome shotgun sequence.
ACCESSION   DBERYS010000024 DBERYS010000000
VERSION     DBERYS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 28675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.024"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..245
                     /locus_tag="ACL8LA_001189"
     CDS             <1..245
                     /locus_tag="ACL8LA_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGS1812951.1"
                     /translation="TRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLV
                     AQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            701..1879
                     /locus_tag="ACL8LA_001190"
     CDS             701..1879
                     /locus_tag="ACL8LA_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ryptide export MFS transporter"
                     /protein_id="HGS1812952.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            2193..2324
                     /locus_tag="ACL8LA_001191"
     CDS             2193..2324
                     /locus_tag="ACL8LA_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGS1812953.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2345..3895
                     /gene="dltA"
                     /locus_tag="ACL8LA_001192"
     CDS             2345..3895
                     /gene="dltA"
                     /locus_tag="ACL8LA_001192"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGS1812954.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNAHIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3892..5136
                     /gene="dltB"
                     /locus_tag="ACL8LA_001193"
     CDS             3892..5136
                     /gene="dltB"
                     /locus_tag="ACL8LA_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGS1812955.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            5150..5389
                     /gene="dltC"
                     /locus_tag="ACL8LA_001194"
     CDS             5150..5389
                     /gene="dltC"
                     /locus_tag="ACL8LA_001194"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGS1812956.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5382..6650
                     /gene="dltD"
                     /locus_tag="ACL8LA_001195"
     CDS             5382..6650
                     /gene="dltD"
                     /locus_tag="ACL8LA_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGS1812957.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLQQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6805..7245
                     /gene="adcR"
                     /locus_tag="ACL8LA_001196"
     CDS             6805..7245
                     /gene="adcR"
                     /locus_tag="ACL8LA_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGS1812958.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            7245..7949
                     /locus_tag="ACL8LA_001197"
     CDS             7245..7949
                     /locus_tag="ACL8LA_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS1812959.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7942..8748
                     /locus_tag="ACL8LA_001198"
     CDS             7942..8748
                     /locus_tag="ACL8LA_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGS1812960.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAVVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8758..10263
                     /gene="adcA"
                     /locus_tag="ACL8LA_001199"
     CDS             8758..10263
                     /gene="adcA"
                     /locus_tag="ACL8LA_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGS1812961.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11434..12207)
                     /locus_tag="ACL8LA_001200"
     CDS             complement(11434..12207)
                     /locus_tag="ACL8LA_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1812962.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            12383..13786
                     /locus_tag="ACL8LA_001201"
     CDS             12383..13786
                     /locus_tag="ACL8LA_001201"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HGS1812963.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13901..14539
                     /locus_tag="ACL8LA_001202"
     CDS             13901..14539
                     /locus_tag="ACL8LA_001202"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGS1812964.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            14551..14991
                     /locus_tag="ACL8LA_001203"
     CDS             14551..14991
                     /locus_tag="ACL8LA_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HGS1812965.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14984..15415
                     /locus_tag="ACL8LA_001204"
     CDS             14984..15415
                     /locus_tag="ACL8LA_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1812966.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15406..15876
                     /locus_tag="ACL8LA_001205"
     CDS             15406..15876
                     /locus_tag="ACL8LA_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1812967.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15901..16674
                     /locus_tag="ACL8LA_001206"
     CDS             15901..16674
                     /locus_tag="ACL8LA_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1812968.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16671..17471
                     /locus_tag="ACL8LA_001207"
     CDS             16671..17471
                     /locus_tag="ACL8LA_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1812969.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17530..19824
                     /locus_tag="ACL8LA_001208"
     CDS             17530..19824
                     /locus_tag="ACL8LA_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS1812970.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELCIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19834..22950
                     /locus_tag="ACL8LA_001209"
     CDS             19834..22950
                     /locus_tag="ACL8LA_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HGS1812971.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            23007..24773
                     /locus_tag="ACL8LA_001210"
     CDS             23007..24773
                     /locus_tag="ACL8LA_001210"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGS1812972.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            24919..26070
                     /locus_tag="ACL8LA_001211"
     CDS             24919..26070
                     /locus_tag="ACL8LA_001211"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGS1812973.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            26215..27099
                     /locus_tag="ACL8LA_001212"
     CDS             26215..27099
                     /locus_tag="ACL8LA_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS1812974.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(27136..27294)
                     /locus_tag="ACL8LA_001213"
     CDS             complement(27136..27294)
                     /locus_tag="ACL8LA_001213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1812975.1"
                     /translation="MKIKEQTRKLATGCSKHRFEVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            27498..27749
                     /locus_tag="ACL8LA_001214"
     CDS             27498..27749
                     /locus_tag="ACL8LA_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGS1812976.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            27737..28027
                     /locus_tag="ACL8LA_001215"
     CDS             27737..28027
                     /locus_tag="ACL8LA_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1812977.1"
                     /translation="MGALGYYEGFVPYVSNQYQNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(28231..28338)
                     /locus_tag="ACL8LA_001216"
                     /pseudo
     CDS             complement(28231..28338)
                     /locus_tag="ACL8LA_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         9044 a         4709 c         6038 g         8884 t
ORIGIN      
        1 gtactcgcct acgagtgatt atctggaagc aatggaagaa gaaatcgaga cgattatggg
       61 gattgcttaa gttaggggtt cctaaatgga tagcagataa ggtatctggc tggggcgacc
      121 attatcaatt agtagctcag aagtcggtac ttaaacgtgc tatatcaaaa ccagtcctgg
      181 aaaaacgtgg actggtttcg tgtttggatt attaccttga acgacatgcg ttaaaagtta
      241 gttgaaccgc tgtatgccga acggcacgta cggtggtgtg agaggggcta gagattatcc
      301 cctactcgat taactcccct gaaatttatt ttaattatgc aaatttcacg tatttttgat
      361 gctgagacga cgatcctggg aacttttcag atattttttt gactatctaa atctatcatt
      421 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccag
      481 tctctttttc gattgaagat gtaattattc tactaactaa ctaacttctt agtactagcc
      541 aacaacgata atcataattc ctcctaaaat taggaataat aaaggcaata gtttttgttt
      601 tttcatgtaa aaaacctcac ttttgttttc tgctatttta tgctaaaata ttaaaaatca
      661 aatttaattc caaagtttgt aactaaaggg ggagcgctac atgtctaatt catttgtcaa
      721 gttgttagtc tctcaattat ttgcaaattt agcagatatt ttctttagag taacaatcat
      781 tgctaacata tacattattt caaaatcagt aattgccaca tcactagttc ctatcttaat
      841 aggaatatcc tcttttgttg cgagtctttt agttccgttg gttactaaaa ggttagcgct
      901 aaatagggtt ttatctttat ctcaatttgg aaagactata ttattggcga tactggtagg
      961 aatgtttacc gtaatgcaat ccgtagcgcc tttggtgacc tatctatttg ttgttgcaat
     1021 ttccatacta gatggttttg cagcacctgt ttcctatgct attgtgccac gctatgcgac
     1081 cgatttgggt aaggctaatt cagccttatc aatgactggt gaagctgttc aattgatagg
     1141 ttggggatta ggtggactct tgtttgcaac aattggtctg ttacctacca cgtttatcat
     1201 tttagtcttg tatatcattt ctagctttct gatgttattt cttcctaacg ctgaagtgga
     1261 ggtgttagag tcagaaacta atcttgaaat tttgctcaaa ggttggaagt tagttgctag
     1321 aaatcctaga ttaagacttt ttgtatcagc aaatttattg gaaatttttt caaatacgat
     1381 ttgggtttct tccattatac ttgtttttgt aacggagtta ttaaataaaa cggaaagtta
     1441 ctggggatat tctaatacag catactctat tggtattata attagtggct taattgcttt
     1501 taggctatct gaaaagttcc ttgctgctaa atgggaaagt attctttttc ctcttgtagc
     1561 tatggcaata gtgacactga ctattctata ttttccaaac gcacagatgt ttttattatt
     1621 ttcagcttta gttggtatgt tatcgcaact aaaagaagtt cctgaaagtg tatttcttca
     1681 ggaaacagta gaggaaaatc atttagttaa tgtctattcc gttcttgaag tgatttctac
     1741 attagcattt tcagtatttg ttttgctaat gagctatatt actgagagtt ttggtattag
     1801 catcagtttt tggctatcag ccatttgtct gatgatagaa gctattttaa tttatatcag
     1861 acgagattat tttaaatgag agagtggtta aaaagtcttt aaaaatcaga aaagcgcata
     1921 gtatcaggtg ttgaaaaaac ttgatattgt gtgttttatt atgggaagat ttacttcatt
     1981 gtctcctgaa atggagtttt gaagcaggat cactctattt gaaaatatac atagaaaaac
     2041 tgtataatcc ttaaaataag ctgaaatcta ttggaaacat acggtaaaca attgaaaaga
     2101 tactatataa agtggtacaa tggtagaaaa ataagctagt aagaatcact cttattttag
     2161 cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca gagaaaagaa ttgtacctct
     2221 ttcttggtcg gacagccttg tattttctta tctttctagg gctgctttac ttctttagct
     2281 atcttggtca gggtcaagga agctttatct ataatgaatt ttaacttgaa ggagaatccc
     2341 tattgtgtca aataaaccaa tagcagatat gattgaaacc attgagcatt ttgctcagac
     2401 acagcctagc tatcctgtct ataatgtttt ggggcaggaa cacacttatg gcgatttaaa
     2461 ggctgattcg gatagtttgg ctgcagtcat tgaccaacta ggcttgcctg agaagtctcc
     2521 tgtggttgtt tttggtggcc aagaatatga aatgttggca acctttgtag cgctgactaa
     2581 gtcaggtcat gcctacattc caattgatag ccattcggcc ttggagcgag tttcagctat
     2641 tttagaagta gcagagccaa gcttgattat tgccatttca gcctttccct tggagcaggt
     2701 ttctacacca atgataaatc tagctcaggt tcaagaagcc tttgcccaag ggaataacta
     2761 tgaaatcacg catccagtca agggagatga taattactac attatcttta cttctggtac
     2821 gactggtaag cctaagggag tgcagatttc acatgataat ctcctcagct ttacaaactg
     2881 gatgattacg gataaggaat ttgcgacacc gagtcgtccg caaatgctgg cacagccacc
     2941 ttattctttt gacttgtctg tcatgtattg ggcaccgacc ttggcacttg gtggtacgct
     3001 tttcactctt ccttcagtca tcactcagga ctttaagcaa ctctttgcgg ctatcttttc
     3061 attgccaatc gctatctgga catcaacacc atcctttgca gatatggcca tgttgtctga
     3121 atacttcaac agtgagaaaa tgcctggaat cactcatttc tactttgatg gtgaagaatt
     3181 gacggtcaaa acagctcaaa aactgcgcga gcgtttccca aatgcccata tcatcaatgc
     3241 ttacggccca acagaagcga cagtagctct gtcagcagtt gccgtgacag acgagatgtt
     3301 agcgactctc aaacgcctac caatcggcta taccaaggct gattctccaa cctttatcat
     3361 tgacgaggaa ggaaataaac tgccaaatgg tgagcaggga gaaatcattg tttctgggcc
     3421 agctgtttca aaaggttata tgaacaatcc tgaaaaaaca gcagaagcct tctttgagtt
     3481 tgaaaatctg ccagcctatc acacaggcga tgtgggaacc atgacagatg agggcttgct
     3541 tctctacggc ggacgcatgg acttccagat taagttcaac ggttaccgca ttgagttaga
     3601 agatgtctct caaaacctca acaagtctcg ctttatcgaa tctgctgtcg cagtaccgcg
     3661 ctataacaag gaccacaagg tacaaaatct attggcttat gtcatcttaa aagacggtgt
     3721 tcgtgagcag tttgagcgag atatcgatat taccaaggcc atcaaggaag acctgacaga
     3781 catcatgatg tcctacatga tgccatctaa attcctttac cgagacagtt tgccactaac
     3841 tccaaatgga aagattgaca tcaaaggatt gattaacgag gtgaataaga gatgatggag
     3901 ttttttcaac agcttcctca tttagagcca tatggtaatc ctcagtattt tgtttatgtg
     3961 attgctgcaa ccttgcccat ctttataggc ctctttttca agaaacgctt tgcctggtat
     4021 gaagtgttgg taagtctctt ctttattgtc accatgttgg tgggtggaaa gaccaatcaa
     4081 ctagctgcct tgggtattta cctttgctgg gaaatattgc tcctgctttt ctacaagcat
     4141 tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag ttagttttct gtccctactt
     4201 ccgattatct ttgtcaaggt gcaaccagct atcaatggaa cgcagtcttt gcttgggttc
     4261 ttgggaattt cttacctgac ctttcgttcg gttgggatta tcatcgagct gagagatgga
     4321 gtgattaagg attttaccct ctgggaattc ctccgtttcc ttctcttcat gccaactttc
     4381 tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa attatcagac cattcctgag
     4441 cgagatgagt tgatggatat gctggatgaa tctgtccgct atatcatgcg gggctttttg
     4501 tataagttta tcctagctca tgttttagga gagaccttac tacctcctct gaagaattta
     4561 gccttgcagt caggtggctt ctttaatctc tatgccttgg cagttatgta tacttttggt
     4621 ctggagctct tctttgactt tgcaggttat tctatgtttg ctttggccat ctcaaacttg
     4681 atgggaatcc gtagccctat caactttaac aagccctttt tatcaaggga tttaaaggag
     4741 ttttggaatc gctggcatat gagtctgtcc ttctggttcc gtgactttgt ctttatgcga
     4801 atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc gtaatgtaac ctcaagcatg
     4861 gcctacattg taaatatgct gattatggga ttttggcatg gcgtgacctg gtactatatc
     4921 gcctatggac tctttcatgg actaggtttg gtcatcaatg atgcctgggt tcgcaagaaa
     4981 aaaacgctca ataaggaacg gaaaaaagca gggaaggctg ccctacctga gaatcgctgg
     5041 attcagttgc ttggcatggt tgtcactttc catgttgtca tgttgtcatt cttaatcttt
     5101 tctggattct tgaataatct atggtttaaa aaataaaagg aaataaaata tggatatcaa
     5161 atcagaagtt atcgaaatta ttgatgagtt gtttatggaa gatgtttctg acatgatgga
     5221 tgaagatctt tttgatgcag gtgtcttgga tagtatggga acggttgagt tgattgtgga
     5281 gattgagaac cgttttgaca ttcgtgtccc tgtaacagag tttggtcgtg acgactggaa
     5341 tacagctaat aaaattatag ctggtattgt ggagctacaa aatgcttaaa cgcttatgga
     5401 tgatcttcgg accggtcttg atcgctggtt tgttggtttt tctgctcatt ttcttttatc
     5461 ctactgagat gcatcataat ctaggagctg aaaagcgttc agcagtggct actactatcg
     5521 atagttttaa ggagcgaagt caaaaagtca gagcactatc tgatccaaat gtgcgttttg
     5581 ttcccttctt tggctctagt gaatggcttc gttttgacgg tgctcatcct gcggtattag
     5641 ctgagaaata caatcgttcc taccgtcctt atcttttagg acagggggga gctgcatcgc
     5701 ttaaccaata ttttggaatg caacagatgt tacaacagct ggagaataaa caagttgtgt
     5761 atgttatctc acctcagtgg ttcagtaaaa atggctatga tccagcagcc ttccagcagt
     5821 attttaatgg agaccagttg actagttttc tgaaacatca atctggggat caggctagtc
     5881 aatatgcagc gactcgctta ctgcaacagt tcccaaacgt agctatgaag gacctggttc
     5941 agaagttggc aagtaaagaa gaattgtcga cagcagacaa tgaaatgatt gaattattgg
     6001 ctcgttttaa tgaacgccag gcctcctttt ttggtcagtt ttcggttaga ggctatgtta
     6061 actacgataa gcatgtagct aagtatttaa aaatcttgcc agaccagttt tcttatcaag
     6121 caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac ttccaataat gagatgggaa
     6181 tggaaaatta tttctataat gagcagatca agaaggattt gaagaaatta aaggattctc
     6241 agaaaagctt tacctatctc aagtcgccag agtataatga cttgcagttg gttttaacac
     6301 agttttctaa atctaaggta aacccgattt ttatcattcc acctgttaat aaaaaatgga
     6361 tggactatgc tggtctacga gaggatatgt accaacaaac ggtgcagaag attcgctacc
     6421 agttagaaag tcaaggtttt accaatatag cagatttttc taaggacggc ggggagcctt
     6481 tctttatgaa ggacaccatt caccttggtt ggttgggttg gttggctttt gacaaggcag
     6541 ttgatccttt cctatccaat cccacaccag ctccgactta ccatctgaat gagcgctttt
     6601 tcagcaaaga ttgggcgact tatgatggag atgtcaaaga atttcaatag ataatataat
     6661 atagaagagt tcaagaatga aactagtttt cacgtttttt cttgactctt tttttgcttg
     6721 tgatataatt aactggtaaa caaaatgtca aagaatagta tttttctctt aaaaaagaga
     6781 agtctaaaag gaaaggagtc agatatgaga cagctagcaa aggatatcaa tgcttttttg
     6841 aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc taataggtca ttgcactagc
     6901 gaggtggccc tgaccaatac tcaggagcat atccttatgc tcttgtcaga ggaatcttta
     6961 acaaattcag aattggctcg tcgtctcaat gtcagtcagg cggcagttac caaggccatt
     7021 aagtctttgg tcaaggaagg gatgttggaa acatctaaag attctaaaga tgcgcgtgtg
     7081 attttttatc agttgactga cttggctcgt ccaatcgctg aggagcacca ccatcaccat
     7141 gagcatacac ttttaaccta tgaacaagtg gcgactcagt ttactccaaa tgaacaaaaa
     7201 gtgattcagc ggtttttgac tgctttagta ggagaaatca aataatgaga tatattacgg
     7261 tagaggattt gtccttctat tatgataagg agcctgttct tgaacatatc aattattgtg
     7321 ttgatagtgg ggaatttgtt accttgactg gggaaaatgg agcggctaag acgacgctca
     7381 tcaaggctag tcttggaatt ctgcaaccac gcattggaaa ggtggctatt tcaaagacaa
     7441 atacgcaagg taagaaattg agaatagcct atcttcctca acaaattgcc agttttaatg
     7501 ctggttttcc aagtacggtc tatgaatttg tcaagtcggg tcgctatccg agaaaaggct
     7561 ggttccgtcg tttgaatgct catgatgagg agcatatcaa ggctagtctg gactcagttg
     7621 gcatgtggga acatcgagac aaacgcttgg ggtctctatc tgggggacaa aagcagcgag
     7681 cggtaattgc gcgtatgttt gcttctgacc ctgatgtgtt tatcctagac gagccgacaa
     7741 cggggatgga tgcaggaagt aaaaatgaat tttacgaact catgcaccac agcgcccatc
     7801 atcatggcaa ggctgttttg atgattaccc atgaccctga agaagttaag gattatgcgg
     7861 atcgcaatat tcatctagtc cgtaaccaag actcgccatg gcgttgtttc aatgttcatg
     7921 agaatggcca ggaggtgggc catgcttagt ttattatctt acgactttat acagcgcgcc
     7981 tttttggcgg ttattgctat gagtcttttc tcaccggtat tgggaacctt cctcatcttg
     8041 cgtcgtcaga gtttgatgag tgataccctt agccacgtct cactttcagg tgtagccttt
     8101 ggtctggttt tggggatttc tccaactgtt tctactattg ccattgtctt gattgcggcg
     8161 gtctttctgg agtatctccg tacggtttac aagagcttta tggaaatcgg gacagctatc
     8221 ctcatgtcaa caggtctggc tgtttctctg attgtcatga gcaagggtaa aagctcgagt
     8281 tcaatgagtt tggaccaata tctctttggt tcgatcgtga ctatcagtga agaacaggtc
     8341 atttccctct ttgtcattgc ggtggttgtt ttgattttga cctttctctt tcttcgtcct
     8401 atgtatatct taacttttga cgaagatacg gcctttgtgg atggcttgcc agttcgtacc
     8461 atgtccattc tttttaacat ggtgacaggg gtggctattg cccttatgat tcctgcagca
     8521 ggagctcttc tggtatcgac cattatggtc ttgccagcta gtattgccct gcgtctgggg
     8581 aaaaacttta aatcggttat gctgcttgcc agtgcgattg gctttttggg aatggtagca
     8641 ggactttaca tttcctacta tgcagaaaca cctgcaagtg caagtattac cattattttt
     8701 gtaactgtct ttatactaat cagtttagta agacgtttta tcaaatagga gacgaacgtg
     8761 aaaaaaatta gcttattact agccagtcta tgtgccttgt ttttagtggc ttgttccaat
     8821 caaaaacagg cagatggtaa actcaatatc gtgacaacct tttaccctgt ctatgaattt
     8881 accaagcaag tcgcaggaga tacggctaat gtagaactcc taatcggtgc tgggacagaa
     8941 cctcatgaat acgaaccatc tgccaaggca gttgccaaaa tccaagatgc agataccttc
     9001 gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat tgctagatac cttggataag
     9061 aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc tcttgccagg tggcgaggaa
     9121 gaagagggag accatgacca tggagaagaa ggtcatcacc atgagtttga cccccatgtt
     9181 tggttatcac cagttcgtgc cattaaacta gtagagcaca tccgcgacag cttgtcagca
     9241 gattatcctg ataaaaaaga gacctttgag aagaatgcag ctgcctatat cgaaaaattg
     9301 caagccttgg ataaggctta cacagaaggc ttgtctcaag caaaacaaaa gagctttgtg
     9361 actcaacacg cagcctttaa ctatcttgcc ttggactatg gactcaaaca agtcgcaatc
     9421 tcaggccttt ctccagatgc agagccatca gctgctcgct tggcagaatt gacagagtat
     9481 gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa atgcctcaca agcccttgct
     9541 aacacacttt caaaagaagc aggtgtcaaa actgatgtcc tcaatccttt agaaagtctg
     9601 acagaagagg acaccaaggc tggagaaaac tacatttccg tgatggagaa aaacctcaag
     9661 gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg aacctgaaaa ggcagaggat
     9721 accaagacag tccaaaatgg ttacttcgag gatgcagctg tcaaggaccg caccttgagt
     9781 gactatgcag gtaactggca atcagtttat cctttccttg aagacggcac gtttgaccaa
     9841 gtctttgact acaaggctaa gttgactggt aagatgaccc aggctgagta caaggcttac
     9901 tatacaaaag gctatcagac agatgtgact aagattaaca ttactgataa tactatggaa
     9961 tttgttcaag gtggacaaag caagaaatac acttacaagt atgtcggtaa gaaaattttg
    10021 acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg aagccacaga tgctgacgct
    10081 ggacaattca aatatgttca gtttagtgac cacaatgttg ccccagttaa ggcagaacat
    10141 ttccatatct tttttggagg tacaagccaa gaagctctat ttgaagaaat ggataattgg
    10201 ccaacctact acccagataa cctatctggc caagaaatcg cccaagaaat gttggcgcat
    10261 tgatgagaaa tcgactagtt cataagagct agtcggtttt ttgatactag atggctaact
    10321 tcattataga cctttcagaa ctaagggaac ctgaaaaaat aaataaaact cttgaaatat
    10381 ctggacttat atgctagaat gtagttagtg tataaggttc attagaacac caaagcccct
    10441 aactagtgcc atagttaagg gcttttttga cgtatcttag cgatttaatg ggtgtttata
    10501 ggctagtcag tcggtctact tcttaccatc tagccatttg cagacgaaat acaagataat
    10561 tccagctata acatctgcta taatagtaag agcaagctct ggtataaggc tcattagttt
    10621 cacctcctct cacgaaccca taggaacgta atcggtaacc gatgacaaaa gtattatatc
    10681 acactaaatt tagatcatca acctcactta atgcttgaac tattgtagat tatatgctaa
    10741 aatgtagtta attgataagg ttcattagaa caccaaagcc cctaactatg gccgtagtta
    10801 agggcttttt tgacgtatct tagcgattta acgggtgttt ataggctagt cactcggtct
    10861 acttcttact atctagccat ctgcagacga aatacaagat aattccagct ataacatccg
    10921 ctataatagt aagagcgagc tctatgataa ggctcattag tttcacctcc tctcacgaac
    10981 ccataggaac gtaatcggta accgatgaca aaaatagtat accacaatac atttagatca
    11041 tcaaggtcac ttaattcttg aaatataggg tttaggcgac aaagtcttta aaatcagaaa
    11101 aacgcataat atcaggtgtg caaaaacttg atactatgcg ttttattgtg ggaaggttta
    11161 ctccattttc tcctgaaatt gagtttttgt ccagcctctg tttttagggt tgctgagaaa
    11221 ataatgtcat gtggtgaata tttgtaaatc agtcagcaga cagaacgaat actcttcgaa
    11281 aatctcttca catcatgtca gcttcgtctt tccgtatata tgtgactgac ttcatcagtt
    11341 ctatctacaa cctcaaaaca gtgtttcgag ctgactttat cagttttcaa atctgtactt
    11401 tgagcaagct gagactagct tcctatttga ttttcattga atatcagaaa cccattctcc
    11461 atcaaataat tcgactgcgt ctaataattt ttgatctggc acggtgtctg aaataaaggt
    11521 tgtgtatttg gagaggggat taattttaaa aaatccagtc ttgtaaaatt tagaactatc
    11581 aatcagtaag atggtttcat gggctttgtc aataatattc ttttttgaaa tagcttggct
    11641 gagagaagct tcataaacat attggtcatc aatacctctt gctgaacaaa atgctaaatc
    11701 gatattaaaa tgatctaata aagaattttc cttatcatag ttgaccacgg aacaggattg
    11761 atgtttgacc tcgccagatg tgataaagat tttggagcta tctttaacag tttcagatag
    11821 ggtttgtgca gtatgtaaac catttgtaaa aataatcaaa ttatcaagtt cagaaagata
    11881 gggacaaagt tcgtagacag tagtactgga atccagataa acacacatac cagaccgaat
    11941 aaagtcttta gcgagactag cgattagtct tttttgccta gtactttctc cttcccgtat
    12001 ttgatgagaa agttcaattg tgttcataga ggacagggtc acgtatccgt gctttctttt
    12061 gataagacct tgattttcta agaaaattaa atcacgacgt aaggtacttg tgctggagaa
    12121 agtgatttct gccagctctt ttacggcaat tctttttttc tttttaataa tttcaatcaa
    12181 ttcaagtaca cgttcatctt ttatcataag ctcctcctaa tttatcattt caactatatt
    12241 atagcacaaa ttggaggaat ttgaattatt tttatgaata ttgggttaac atttgaacat
    12301 tattcaagta agcgttcaca tattgaaaaa ataaaatgtg gggattataa taaagttaat
    12361 caaggacgaa gagagaagaa aaatggaagc ggttttagca atagatttag gtgcgacttc
    12421 tggaagagca atcgttggtt acctttctga aaataaacta gtaatggaag aaataaatcg
    12481 cttttctaat ctacctatta gagtaaaagg gcatttatct tgggatattg actttctact
    12541 agctaaaatt cttgaaagta tccgcttggc taatactagt tacaagattt tatctatcgg
    12601 tattgacaca tggggagttg attttggact gattgataat gaaggtaagc tgttatcaca
    12661 acctgttcat tatcgtgatg aaagaacaaa gggagtgtta aaggaaatat ctgaaatgac
    12721 tgaattagaa aaactgtatt cagagacagg aaatcagatt atggagataa ataccttgtt
    12781 tcaactcttt aaggcacgtc aagaatctcc tgactctttc tataagacca ataagattct
    12841 tttaatgcca gatttgttta attatctctt gacaggtaag tttgctacag aaaaaagcat
    12901 tgcttcaaca actcaattat ttgatcctag gagtcaaaat tggaatcaga atatcttaaa
    12961 actatttgaa ttggattcat ctttacttcc tgaaattgtt tcagagggaa atgttcttgg
    13021 aaggataaaa aaggagtatg gtttaggcga tattcctgtt gtgaatgttt gtagtcatga
    13081 tacagcaagt gcgattgtct cagtacctaa gacagaaggt agtttattta tttcatcagg
    13141 tacttggtct ttggttggag tggaacttac ttcaccgatt cttactaccg aatccttcag
    13201 ttatggattt acaaatgaag tcggtaaaga tggagagatt acatttctga agaattgtac
    13261 agggttgtgg atcatagagg aactaagacg ttcatttgaa cgaagaggga aagcctattc
    13321 ttttgatgat attaggacaa tggtggagaa agaaaaagaa aatcttcctc tgattgatac
    13381 tgaatcaact gaatttgcaa cagaatctga tatgcacaag actttgacag aatatctagc
    13441 ttatcatcat gaaactagag agtggacaga tggacaacta tttaagattg tttatgaaag
    13501 cctagctgaa acgtatagga aagcgataga gttactagaa gaactaactc ataaggttta
    13561 taagaggata tatgtgattg gaggaggtgc tagagccagt tactttaacc aaatgattgc
    13621 tgatagaact ggtaaagagg ttcttacagg ttcgactgag ggtacagctg tggggaatat
    13681 tgttgtgcag ctcatagcta tgggacaatt aaaagggatg gaagaagctc accatgttat
    13741 tgaggagttt ctacaattag agagttatta ctcccaaaag aattaaaaag attgagagtt
    13801 tgtaaatttg cctccctccc ccttcttaag ttttgtacaa gaagggggga taattggtga
    13861 attgaaaaat atttagtgtt ttgatatgag gaggacaaga atgtcagatg taaaacaaga
    13921 attaattaag tatggcaaga agctagtaga aacagatttg acgaaaggaa caggtgggaa
    13981 tctcagtgtt ttcgatcgtg aaaaacaatt gatggcaatt accccgtcgg gtattgattt
    14041 ctttgaaatc aaagaatccg atattgtagt gatggatatt aatggaaatg ttgtagaggg
    14101 agaacgcttg ccatctagcg aatggtatat gcatttgatt caatatcaaa ctcgtgatga
    14161 tatcgatgca attatccatg ctcatacaac ttatgcaaca gtattagctt gtctcagaga
    14221 accacttccg gctagtcatt atatgattgc agtggcaggg aaagatgttc gggtagctga
    14281 gtatgcaaca tatggtacga aagaattggc tgtgaatgca gctaaagcaa tggaaggtcg
    14341 tagagcagtt ttactagcga atcatggaat tttagcaggt gcacaaaatt tattgaatgc
    14401 atttaatatt gttgaagaag ttgaatattg tgcaaaaatt tattgtttag ctaagaattt
    14461 tggagagcca gtagttcttc ctgatgagga gatggattta atggcagaaa aatttaaaac
    14521 atacggtcag agaaaatagg gaggatatta atgttaaaac atataccgaa aaatatttct
    14581 ccagatttat tgaagacttt aatggaaatg ggacatggag atgaaatagt attagctgac
    14641 gcgaattatc cttctgcctc atgtgcaaat aagctaattc gttgtgatgg cgtaaatatt
    14701 ccagaattat tagattccat tctgtattta atgccattag atagttacgt cgatagttca
    14761 attcagttta tgaacgttgt ttcgggtgat gatattccta agatatgggg tacctataga
    14821 cagatgattg aaggtcatgg tacagatctt aaaacgatta cttatcttag aagagaagac
    14881 ttttatgaac gtagtaagaa agcttatgct attgttgcta caggagaaac ttcactttat
    14941 gctaatatta tccttaagaa aggagtagtt gttgaaagag aaaatgttta atagaggaat
    15001 tttagttgcc agtcatggta attttgctag cggagctctc atgaccgcag aaatgtttgt
    15061 tggtgagaca acaaatgata gagttaggac attaggtttg atgcctggag agaatattgt
    15121 agagtttgaa cattatttta aaaatcaagt aaatgaactg ttagactcaa atcaagaggt
    15181 tatcgttttg actgacttga ttggaggaag tcctaataat gtggctttgt cacggttttt
    15241 aaatttggat tcagttgata ttgtaacagg gtttaatatc cctctcctag tggaattaat
    15301 atcaagttat gattcaaaaa tcaatttaga agaaattgtt caaaatgctc aaaatagttt
    15361 gtttaatgtt aaacaacaac ttaacgtaga ggaggaagag gatttatgtc tatagagttt
    15421 gttcgtattg atgaccgtct ggtacatggt caagttgtca ctacgtggct aaaaaagtat
    15481 gatattgagc aagttatcat tgttaatgat cgcatctcag aagataaaac acgacaatct
    15541 attttaaaga tttctgcacc ggtaggttta aaaattgttt tctttagtgt aaaacggttt
    15601 gtggaagttt taaactctgt gccaataaaa aagagaacaa tgctgatata tacaaatcca
    15661 aaagatgtgt atgattctat tgaaggaaat ttaaaattgg agtacctcaa tgtaggacag
    15721 atgagtaaaa cggaggaaaa tgaaaaggta acgggaggtg tagctctagg tgaagaagac
    15781 aaatattatt ttaagaaaat agttgataag ggaacgagag ttgaaattca aatggttcct
    15841 aatgataaag ttacaatgtt ggaaaaattt ttataaaaat aatttaagga ggtacagtat
    15901 atgctattca cacaagcatt actggtgaca ttagttggga ttattgccac tattgactat
    15961 aatggaccgt tatttatgat tcaccgtccg ttagttacaa gtgcaatggt tggcttagtc
    16021 ttaggagatt tcacccaagg tgttcttatt ggttcagctc ttgaattaac ttggctcggt
    16081 gtaacaggta ttggaggtta cactccacca gatactattt caggtgcgat tattggtact
    16141 gcatttggta ttttgtctgg tcaaggagaa actgctggta tcgctatagc agttccaatt
    16201 gcagttgcta ctcaacagtt ggatgttctt gcaaaaactt tagatgttta ttttgtgaaa
    16261 aaagctgata atgatgctaa aaacggagat tattcaaaga tcggttttta tcattattca
    16321 agtttggttt taatcacttt atttaaaatt gtaccaattt tcctagctat tatgcttgga
    16381 ggggaatatg tggcagactt gtttgctaag gttccaccaa tcgttatgca gggacttaac
    16441 tctgcaggtg ctttactacc ttcaattggt tttggtatgc ttttaaatat gatgctcaag
    16501 aaaaatatgt gggtattctt gttgattgga ttcatttgtt ctgtgtatgg aggaatgtca
    16561 accattggga tctcactagt tggtattgcg gtagcatact tctacgatat gattggaagt
    16621 aaaccacaag aaacaacttc aagtagtgat gttgaggagg atcttgatct atgatgaata
    16681 ataaagtaac taaagttgaa cttaaaaaag ttttcaaacg aagttttatg tatggttctt
    16741 catggaacta tgagagaatg cagaacctag gttttctata tacaattctt ccagtattga
    16801 aaaaactata cccagacaaa gattcagctt ctcctgcaat gaaacgtcac cttgagtttt
    16861 tcaatactca tcaaacagcg gcaccattta ttcttggagt tacttccgct atggaagaac
    16921 aagaaggaaa tgaaggtgca gcttcaatta ctggtattaa agttggcttg atggggccac
    16981 tggctggtct aggagatagt ttgttctggc tgacactagt tcctatctgt tttagtattg
    17041 gtgcgtctta ttctaaagac ggcggtgctt taggtatctt tatcgcctta atattgttta
    17101 atattattaa tattcctgtt aaatatttcg gtttgaaata tgggtatact aagggttcta
    17161 gtcttatcca agaaaataat acaaaaggaa cattgaatcg cgttacgagt atggcgacag
    17221 cattagggct agtactagtg ggtggtttga ttccatcaat ggttggtatt aattttggat
    17281 tagaatttaa gcagggggaa cttgttattt ctgttcaaga aatgattaca aaattaattc
    17341 caggatttat ccctatggct ttgactttat taatgtgtaa attaattaga aaaggaaaga
    17401 atccggttgt actaatcttt agtgttatgg ctattggagt tatcctagtt gttttaggaa
    17461 ttttgaagta gtagaaagtg tggaggtggt atttgggata tcacctccat tttggaagag
    17521 aggtaaagag tgaaattatg gtataagaaa gctgccgcaa attggaatga agccttgccg
    17581 attgggaacg gtcatttagg tggtatgatt tatggttcag ctacaaaaga atgtattcaa
    17641 ctaaacgatg agactatttg gtatagagga aagtcagata gaaataatcc agactcacta
    17701 ttgcatctta aaaaaattcg ggaatatctt ttagatggag aaattcagaa agccgaagaa
    17761 ttgataaagt taacaatgtt tgctacccca agagatcaaa gccactatga attacttggg
    17821 gaactttgca ttgagcatat agatattcag tcttgtgctc tttcattgta tgaaagagag
    17881 ctagatttag atacagctat ttctaatgtt gtgtttgagc ctaatagttg taatttacaa
    17941 ataaaaagag aatattttac gagttttaat aagaatattt tatgttgccg tatagtgtca
    18001 tcagttcaaa acacattaaa tttaaacatt aatttgggta gaaataaacg gtttaatgac
    18061 gaagtatcta aactggattc aagtacaatt ttaatgtcgg cctctgctgg aggtagaaaa
    18121 ggtgttcagt ttaaagtagt atgtcattct aaggttacgg atggtgaagt aagtgtattg
    18181 ggagagacaa tagttattcg gaatgctaca gaggtatttc tttatctcaa atcaatgacg
    18241 gattattggg gaaatataga tatttcttct cttcagggag aatttagtag tattgattac
    18301 tttacagaaa aagatgaaca tgtaaaaaaa tatcaggagc aatttaatag agttgatttt
    18361 aaactagact atagtaaagg ttgtcttagc attccaacga atctacttct tgaaaacact
    18421 aaaaagtata gtaactactt gactaacttg ttatttcatt atggaagata tctgttaata
    18481 tcgtctagtc aaccgaatgg tttacctgcc aatcttcaag gaatatggtg tgatgaatta
    18541 aatccaattt ggggttctaa atatacgatt aatattaata ctcaaatgaa ttattggatg
    18601 gtaggtccat gtgatttacc agaagtagaa tatccattat ttgatatgct cgaaagaatg
    18661 agagaaccgg gaagactaac cgctaagaaa atgtatggag ctagaggttt tacagcacat
    18721 cataatacgg atggttttgg cgatacggct ccccaatctc atgccatggg ggctgcaatt
    18781 tgggtattaa ctattccatg gttatgtact catatttggg aacactattt atatttccaa
    18841 gatgagcgta ttcttacgga acattttgaa atgataaaag aagcatttct tttctttgaa
    18901 gattatttat ttgaggtgga tggctacttg atgacaggtc caagtgtctc accggaaaat
    18961 aaatatcgct taaaaaatgg tattgaagga aatgcttgtc tatcatctac aattgataat
    19021 caaattctaa gatatttttg tgattcatgc attggcattg caaaacaatt aggagacaat
    19081 tcggatttta ttagtcgtgt gaaggagtta aaaaagaaat tacctagaac aaaaataggt
    19141 agtaatgggc aaatacaaga atggctagag gattatgaag aagtagagcc tggtcataga
    19201 cacatttcac ctctatttgg gctttatcct tataatgaga ttgatattca taaaactccg
    19261 gaattagcag aagcagctaa aatcactatc aataggagat tatcaaacgc taacttttta
    19321 tcttcacagg atagggagca agcgattaat aattggttag taagtggttt gcatgctagt
    19381 acacaaacag gttggagtgc tgcatggctg attcattttt ttgcgagact atatcaaggt
    19441 gaacctgctt ataaccagat taatggtttg ttaaataatg cgactcttgg caatttattt
    19501 cttgaccatc caccatttca aattgatggt aatttaggtt tggtgagtgg aatttgtgaa
    19561 ttattagtac agagccatca taattggtta tcactaattc cagctttacc ttctgcttgg
    19621 tcagaaggag aagtgaaagg tttcagagta agaggaggat ataaggtatc gtttgcttgg
    19681 aaaaatgggg atataacatt cctaaaattg gaaggaggaa acaaagatca aaaagtaaga
    19741 gtaagaatat atggcaaaaa tactgatgta caaaatattg aattggtatt taattcagaa
    19801 aaaattattg agttaaattt ttaggtataa gtcatgaata aagaaaaaat aaaaagaaaa
    19861 ttaatcacaa tattgtttgt atgtattggg atgttatgtt ttggattgtt agcaggagtt
    19921 aaggctgata atcgtgttca aatgagaacg acgattaata atgaatcgcc attgttgctt
    19981 tctccgttgt atggcaatga taatggtaac ggattatggt gggggaacac attgaaggga
    20041 gcatgggaag ctattcctga agatgtaaag ccatatgcag cgattgaact tcatcctgca
    20101 aaagtctgta aaccaacaag ttgtattcca cgagatacga aagaattgag agaatggtat
    20161 gtcaagatgt tggaggaagc tcaaagtcta aacattccag ttttcttggt tattatgtcg
    20221 gctggagagc gtaatacagt tcctccagag tggttagatg aacaattcca aaagtatagt
    20281 gtgttaaaag gtgttttaaa tattgagaat tattggattt acaataacca gttagctccg
    20341 catagtgcta aatatttgga agtttgtgcc aaatatggag cgcattttat ctggcatgat
    20401 catgaaaaat ggttctggga aactattatg aatgatccga cattctttga agcgagtcaa
    20461 aaatatcata aaaatttggt gttggcaact aaaaatacgc caataagaga tgatgcgggt
    20521 acagattcta tcgttagtgg attttggttg agtggcttat gtgataactg gggctcatca
    20581 acagatacat ggaaatggtg ggaaaaacat tatacaaaca catttgaaac tggaagagct
    20641 agggatatga gatcctatgc atcggaacca gaatcaatga ttgctatgga aatgatgaat
    20701 gtatatactg ggggaggcac agtttataat tttgaatgtg ccgcgtatac atttatgaca
    20761 aatgatgtac caactccagc atttactaaa ggtattattc ctttctttag acatgctata
    20821 caaaatccag ctccaagtaa ggaagaagtt gtaaatagaa caaaagctgt attttggaat
    20881 ggagaaggta ggattagttc attaaacgga ttttatcaag gactttattc gaatgatgaa
    20941 acaatgcctt tatataataa tgggagatat catattcttc ctgtaataca tgagaaaatt
    21001 gataaggaaa agatttcatc tatattccct aatgcaaaaa ttttgactaa aaatagtgag
    21061 gaattgtcta gtaaagtcaa ctatttaaac tcgctttatc caaaacttta tgaaggagat
    21121 gggtatgctc agcgtgtagg taattcctgg tatatttata atagtaatgc taatatcaat
    21181 aaaaatcagc aagtaatgtt gcctatgtat actaataata caaagtcgtt atcgttagat
    21241 ttgacgccac atacttacgc tgttgttaaa gaaaatccaa ataatttaca tattttattg
    21301 aacaattaca ggacagataa gacagctatg tgggcattat caggaaattt tgatgcatca
    21361 aaaagttgga agaaagaaga attagagtta gcgaactgga taagcaaaaa ttattccatc
    21421 aatcctgtag ataatgactt taggacaaca acacttacat taaaagggca tactggtcat
    21481 aaacctcaga taaatataag tggcgataaa aatcattata cttatacaga aaattgggat
    21541 gagaataccc atgtttatac cattacggtt aatcataatg gaatggtaga gatgtctata
    21601 aatactgaag ggacaggccc agtccctttt ccaacaccag ataaatttaa tgatggtaat
    21661 ttgaatatag catatgcaaa accaacaaca caaagttctg tagattacaa tggagaccct
    21721 aatagagctg tggatggtaa cagaaatggt aattttaact ctggttcggt aacacacact
    21781 agggcagata atccctcttg gtgggaagtc gatttgaaaa aaatggataa agttgggctt
    21841 gttaaaattt ataatcgcac agatgctgag actcaacgtc tatctaattt tgatgtgatt
    21901 ctatatgaca ataatagaaa cgaagttgct aagaaacatg ttaataattt gtcgggtgaa
    21961 tctgttagtc tagatttcaa agaaaaagga gcaaggtata ttaaagttaa attactaacg
    22021 agtggagtgc ctttgagttt agcagaagta gaggttttta gagaatcaga tggtaagcaa
    22081 tctgaagagg atatagataa aataacagaa gataaagtag tctctacaaa taaggtagct
    22141 actcaaagtt caaccaatta tgagggtgta gctgctttag cagttgatgg taataaagat
    22201 ggagattacg gacatcattc ggtgactcat actaaggcag attctaacgc ttggtggcag
    22261 gtcgatctgg gagaagagtt tacggtttct aaagttgata tttataatag aacagatgcc
    22321 gaacctcagc gtttatctaa ttttgatgtt atttttctat cttcatcagg agaagaagtt
    22381 tttagaagac attttgataa agtagttgat ggtttgttat ctttaaaagt accttctgta
    22441 ggggctaagc tagtcaaaat agaattaaaa tcagcagcta ttccgttaag tttagcggaa
    22501 gttgaagtct atggttcaaa gagaactccg aagaaacttt ctaatattgc attaaccaaa
    22561 gaaactcgac agagttcaac ggattacaat ggtttttctc gtctagcagt tgatggaaat
    22621 aaaaacggag attatggtca tcattcagtg actcatacca aaggagattc tccttcatgg
    22681 tgggagatag atttagcaca aaccgaagaa ttagaaaagt taattattta taatagaaca
    22741 gatgctgaaa ttcagagatt atcaaatttt gatattatta tatatgattc aaataatcat
    22801 gaagttttta aacaacatat tgacagttta gaaagcaata atctatccat agacttaaaa
    22861 gggctgaagg gaaaaaaggt tagaatttct ttgagaaacg caggaattcc tttaagtttg
    22921 gcagaggtag aggtttatac ttataagtaa ttttaaaaat tatcatcggt agtatgaaag
    22981 aaacagaaaa ataagaggaa aatagtatga ttcaacatcc acgtattggt attcgtccga
    23041 ctattgatgg tcgtcgtcaa ggtgtacgcg aatcacttga agtacaaaca atgaacatgg
    23101 ctaaaagtgt ggcagatttg atttcaagca cattgaaata tccagatggg gaacctgtgg
    23161 aatgtgtgat ttctccatct accattggtc gtgttccaga ggctgcagct tcccatgagt
    23221 tgtttaaaaa atcaaatgtt tgcgcaacaa ttacagttac accatgctgg tgttatggta
    23281 gtgaaactat ggatatgtct ccagatattc ctcatgctat ttggggattt aatgggacag
    23341 aacgcccagg agctgtctat cttgcagctg tactagcttc acatgctcaa aaagggattc
    23401 cagcctttgg gatttatggt agagatgttc aggaagctaa tgatacagat attccagaag
    23461 atgtcaaaga aaaactttta cgttatgcgc gtgcagctct tgcaactggc ttgatgagag
    23521 acactgctta cctatcaatg ggtagtgttt cgatggggat tggtggttct attgtaaatc
    23581 cggatttctt ccaagaatac ttaggaatgc gaaatgaatc ggtagatatg acggagttca
    23641 cacgccgtat ggaccgtggt atttacgacc ctgaagagtt tgaacgtgct atggtttggg
    23701 taaaagaaca tatcaaagaa ggagttgacc gaaatcgtga ggatttgatt ctttctaaag
    23761 aagagaaaga aaaacaatgg gaatttgtta ttaagatgtt catgattgga cgtgacttaa
    23821 tggttggtaa cccaagactt gctgaacttg gttttgagga agaagcagtt ggtcaccatg
    23881 ctttagtagc tggtttccaa ggtcaacgtc agtggacaga ccattttcca aatggagatt
    23941 ttatggaaac tttcctcaat actcagtttg actggaatgg tattcgaaaa ccatttgtat
    24001 ttgcgacaga gaatgattca ctaaatggtg tgtctatgct ctttaattat ctactaacaa
    24061 atactccaca aatctttgct gatgtgcgta cttattggag tccagaggct gttgaacgtg
    24121 taacaggata tactttagag ggtcgtgctg cagctggctt cttacatcta atcaactctg
    24181 gttcttgtac attggatggt acaggtcaag ctactcgaga tggcaaacct gttatgaaac
    24241 cattctggga gttggatgaa agtgaagtac aggctatgct tgaaaataca gacttcccac
    24301 cagcaaaccg cgagtacttc cgtggaggag gattctcaac tcgtttcttg acgaaggggg
    24361 atatgccagt aacaatggta cgtctcaatc ttctaaaagg ggttggtcca gtgctacaaa
    24421 ttgcagaagg ttacacactt gaacttcctg aagatgttca ccatacttta gataatcgta
    24481 cagattcagg atggccaact acttggtttg ctccacgttt gacaggaaaa ggtgctttca
    24541 agtctgtcta tgacgtcatg aataattggg gagctaatca cggagccata acatatggac
    24601 acattggagc agacttgatt accttggctt ctatgttgag aattcctgtc aatatgcata
    24661 atgtacctga ggaagatatc tttagaccta aaaattggtc cttatttgga acagaagatc
    24721 tagaatcagc agactatcgt gcatgtcagt tgttgggacc actacataaa taaaacttgt
    24781 ttatatagga ggtgaactta cgtccctcct atccttttaa aaagatttgt taaacaattc
    24841 acaaataatt gaaaacgaat acaaaaagta atataatgat gttaaataga tagcgcggag
    24901 gcgcaggagg aaaattatat ggctatattt tatgttccgg cagtcaacct tattggaaaa
    24961 ggtgttgtaa atgaagtggg tccttatatc aaggaacttg gctataaaaa ggcacttttg
    25021 gtgacagata agtacatcga aggcagtgat attttaccta agactttaaa accactggat
    25081 acagaaggaa tcgaatatgt catctttagc gatgtagagc caaaccctac ttgtaaaaat
    25141 gtcacagatg gggtagctgc tttgcaagaa catggctgtg actttatcat cagtcttggc
    25201 gggggttctc cacaggatgc agctagttgt atttctatca tggctacaaa tggtggaaaa
    25261 ccacaggatt atgaagggct tcataagtct gctaaaaaag gtttgccagt tgtggctatc
    25321 aatacaacgg caggtacatc agcagaaatt accattaact atgtgattac tgatgaagaa
    25381 cgcaaggtta agatggtaat ggttgacaag aatagccttg ccttgatttc tgtcaatgac
    25441 cctgaactca tgctttccaa acctaaaggc ctgactgctg ctactggtat ggatgctctg
    25501 actcatgctg ttgaagcttt ggtaacacct ggtgcttatg atgtaaccaa gaaactgtct
    25561 attggtgcta ttgagcttat caaggaatat cttcctcgtg ctgtagaaaa tggacatgat
    25621 attgaagcgc gtgaaggtat ggtcaatgcc atcttccttg gtggtatgag ctttaataat
    25681 gctggtcttg gctatgttca ctcaatggct caccaactcg gtgcagtata taatttgcca
    25741 catggcgtgt gctgtgccat gttgctacca gttatagaac gtgaaaatgc taaacgtgta
    25801 ccagaagctt tccgcaatgt tgccaaagcc ttgggacttc atgtagaagg taaatcagat
    25861 caagaatgtg ccgattatgc gattgctgag attgagaagc tttctgagac agtaggtatt
    25921 cctaagaaac tgactgaact tggtattgaa gaaaaagatt tcgactttga atacctttct
    25981 aagaatgcct tgattgatgc ctgcgcacca ggaaatccat ttatgccaac cttagaagaa
    26041 acgattgcct tttataaaga gttattttag caagagattg gacgacaata ggacaattca
    26101 cctctaatta ttgacatttc aggaaaaatc gcagtaaaaa cggcacagcc ttctttaaat
    26161 gtgctataat actagaaaaa tacttgtgga ggttccatta tggcaatatt tttcatgatt
    26221 tttctgattg tttgtgtgct cctattggtg atagtcacac tgagtacagt ttatgtggtt
    26281 cgtcagcagt cggtggcgat tattgaacgc tttgggaaat accaaaaggt tgctaatagc
    26341 ggtattcata ttcgcttgcc ttttgggatt gactcgattg cagcacggat tcagttgcgc
    26401 ttgttgcaaa gtgatattgt ggttgagact aagaccaagg acaatgtgtt cgttatgatg
    26461 aatgtagcga ctcagtaccg tgtcaacgag cagagcgtga cagatgctta ctataaactc
    26521 atacgtccag aatctcagat taaatcttat atcgaagatg ctcttcgctc ttctgttcca
    26581 aaattaacct tggatgaatt gtttgagaaa aaagatgaga ttgcccttga ggttcaacac
    26641 caagtagcag aagaaatgac cacttacggc tacattatcg tgaaaacctt gattaccaag
    26701 gtcgaaccag atgcagaagt taagcaatct atgaatgaaa tcaatgcggc gcaacgtaag
    26761 cgggtcgcag cacaagaatt ggcggaagct gacaagatta aaattgtcac tgcagctgaa
    26821 gccgaagcag aaaaagaccg ccttcatggt gtggggattg cccaacaacg taaggcgatt
    26881 gtggatggat tggcagagtc tatcaccgaa ctcaaggaag ccaatgttgg catgacagaa
    26941 gaacaaatca tgtctatcct cttgaccaac cagtatttgg ataccttgaa tacctttgcc
    27001 tctaaaggaa atcaaaccat ctttttacca aatactccaa atggtgtgga tgatatccgt
    27061 acacaaatct tgtcagccct tcgcgctgag aagaaataat agactaatac tcttcgaaaa
    27121 tctcttcaaa ctacgtcagc gtcgtcttgc cgtatagatg ttactgactt cgtcagttct
    27181 atctgcaacc tcaaaacagt gttttgagct gacttcgtca gtcttatcta ctacctcaaa
    27241 acggtgtttt gagcaacctg tagctagttt cctagtttgc tctttgattt tcattgagta
    27301 taaagagctt ggcaacaggc tctttttgta ttgcgtttat taagaaaaac aaaaaacatc
    27361 gatatacatt taactaaaaa ttattgtatg ttatctccct taattaggaa tgataaggga
    27421 ataactagaa agatttgtga atacaaacta tttctgatat acaaaatata cagtaataat
    27481 gaatgatggg agatgggatg aaagaatttc aatttgagag aaagcagcgt ttttctttga
    27541 ggaaatatgc aataggagct tgttcggtct tgctaggaac gagtttattt tttgctggta
    27601 tgggtgctca gcctgtacag gatacagaaa cgagttcagc actaatttca agtcattatt
    27661 tggatgagca ggatttatct gaaaagctga aatctgagtt gcaatggttt gaattagaaa
    27721 acaagctttt gaacttatgg gagcattagg ttactatgaa ggatttgttc cttatgtttc
    27781 aaatcaatac caaaaccaag ctgaagaaga atacaaaccg ctatctgata aatatatttt
    27841 cgaaaaaatc ttaggaaaaa catatgcagc cttcaaaaaa gatcaaatta atgagcgtgt
    27901 cgagaaatta ggtaagttaa aacctattac aataaattac aacggaaaat cagaagtaat
    27961 tgatagtaaa gaaaaattac aagagcttat gaataaagcc gttaaagacg aagtggctca
    28021 aatatagtaa aatgaaataa gaacaggaca aatcgatcag gacagtcaaa tcgatttcta
    28081 aaaatgtttt agaagtagag gtgtactatt ctagtttcaa tatactataa aatcaggcaa
    28141 taccacggct aaaaagtttg agtttatcga aataccagtg caaaaactga aaaaagcaat
    28201 ttataaagct catctcaaag attcagatga ctttagacca gaaacatcca caccaaatct
    28261 ttttgaaagc tgtttgaagc tttgtccttg ctttcttagt tcatagatct gaactttatc
    28321 ttcataactc aatttcataa taaaacaccc caaaagttag attttttctg tctaactatt
    28381 ggggtgcagt tcaataaatc ttgacccgtt aaatttgtac tgaataaact aaatgaaact
    28441 tttatcccac tcactaatac tattatatca acccactaca gttgacaaag agacaaacca
    28501 gcctaatggc taacttgtct tcaatcaaga gaggattctg ttccccccat ataagcacta
    28561 acattccaac gtacacatat tggaacgaca tccataactt cagagaatcc ctaaagttta
    28621 caatttaaat gaattaacaa ttttcccaac taaaagcact ccagttaccg caacg
//
LOCUS       DBERYS010000025        26289 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.025, whole genome shotgun sequence.
ACCESSION   DBERYS010000025 DBERYS010000000
VERSION     DBERYS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.025"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..899
                     /gene="lytB"
                     /locus_tag="ACL8LA_001217"
     CDS             <1..899
                     /gene="lytB"
                     /locus_tag="ACL8LA_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HGS1812978.1"
                     /translation="KNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDGE
                     KLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGH
                     RFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAE
                     ELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNN
                     FFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASD
                     PYWGEKIASVMMKINEKLGGKD"
     gene            complement(960..2615)
                     /gene="pavA"
                     /locus_tag="ACL8LA_001218"
     CDS             complement(960..2615)
                     /gene="pavA"
                     /locus_tag="ACL8LA_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGS1812979.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIGLDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2720..3217
                     /gene="ybeY"
                     /locus_tag="ACL8LA_001219"
     CDS             2720..3217
                     /gene="ybeY"
                     /locus_tag="ACL8LA_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGS1812980.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHKLNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3198..3593
                     /locus_tag="ACL8LA_001220"
     CDS             3198..3593
                     /locus_tag="ACL8LA_001220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS1812981.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3610..4509
                     /gene="era"
                     /locus_tag="ACL8LA_001221"
     CDS             3610..4509
                     /gene="era"
                     /locus_tag="ACL8LA_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGS1812982.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4558..5382
                     /gene="mutM"
                     /locus_tag="ACL8LA_001222"
     CDS             4558..5382
                     /gene="mutM"
                     /locus_tag="ACL8LA_001222"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGS1812983.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5382..5987
                     /gene="coaE"
                     /locus_tag="ACL8LA_001223"
     CDS             5382..5987
                     /gene="coaE"
                     /locus_tag="ACL8LA_001223"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGS1812984.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            5974..7173
                     /locus_tag="ACL8LA_001224"
     CDS             5974..7173
                     /locus_tag="ACL8LA_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HGS1812985.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7173..7322
                     /gene="rpmG"
                     /locus_tag="ACL8LA_001225"
     CDS             7173..7322
                     /gene="rpmG"
                     /locus_tag="ACL8LA_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1812986.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7362..7595
                     /gene="secG"
                     /locus_tag="ACL8LA_001226"
     CDS             7362..7595
                     /gene="secG"
                     /locus_tag="ACL8LA_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGS1812987.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7697..10051
                     /gene="rnr"
                     /locus_tag="ACL8LA_001227"
     CDS             7697..10051
                     /gene="rnr"
                     /locus_tag="ACL8LA_001227"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGS1812988.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNH
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYVDVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10014..10481
                     /gene="smpB"
                     /locus_tag="ACL8LA_001228"
     CDS             10014..10481
                     /gene="smpB"
                     /locus_tag="ACL8LA_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGS1812989.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10497..11357
                     /gene="tehB"
                     /locus_tag="ACL8LA_001229"
     CDS             10497..11357
                     /gene="tehB"
                     /locus_tag="ACL8LA_001229"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGS1812990.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11430..12383
                     /gene="coiA"
                     /locus_tag="ACL8LA_001230"
     CDS             11430..12383
                     /gene="coiA"
                     /locus_tag="ACL8LA_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGS1812991.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12402..14204
                     /gene="pepF"
                     /locus_tag="ACL8LA_001231"
     CDS             12402..14204
                     /gene="pepF"
                     /locus_tag="ACL8LA_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS1812992.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14206..14919
                     /locus_tag="ACL8LA_001232"
     CDS             14206..14919
                     /locus_tag="ACL8LA_001232"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGS1812993.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            14986..15927
                     /gene="prsA"
                     /locus_tag="ACL8LA_001233"
     CDS             14986..15927
                     /gene="prsA"
                     /locus_tag="ACL8LA_001233"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGS1812994.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16044..16385)
                     /locus_tag="ACL8LA_001234"
                     /pseudo
     CDS             complement(16044..16385)
                     /locus_tag="ACL8LA_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16799..16894
                     /locus_tag="ACL8LA_001235"
                     /pseudo
     CDS             16799..16894
                     /locus_tag="ACL8LA_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(17148..17768)
                     /locus_tag="ACL8LA_001236"
     CDS             complement(17148..17768)
                     /locus_tag="ACL8LA_001236"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1812995.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(17768..18250)
                     /locus_tag="ACL8LA_001237"
     CDS             complement(17768..18250)
                     /locus_tag="ACL8LA_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGS1812996.1"
                     /translation="MAKKVKIKKTLAEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(18250..19092)
                     /locus_tag="ACL8LA_001238"
     CDS             complement(18250..19092)
                     /locus_tag="ACL8LA_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGS1812997.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(19187..19987)
                     /locus_tag="ACL8LA_001239"
     CDS             complement(19187..19987)
                     /locus_tag="ACL8LA_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGS1812998.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            20131..21510
                     /gene="glmU"
                     /locus_tag="ACL8LA_001240"
     CDS             20131..21510
                     /gene="glmU"
                     /locus_tag="ACL8LA_001240"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGS1812999.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            21520..22065
                     /locus_tag="ACL8LA_001241"
     CDS             21520..22065
                     /locus_tag="ACL8LA_001241"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1813000.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            22077..22391
                     /gene="macP"
                     /locus_tag="ACL8LA_001242"
     CDS             22077..22391
                     /gene="macP"
                     /locus_tag="ACL8LA_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGS1813001.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            22408..23100
                     /locus_tag="ACL8LA_001243"
     CDS             22408..23100
                     /locus_tag="ACL8LA_001243"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGS1813002.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            23177..23668
                     /gene="rocS"
                     /locus_tag="ACL8LA_001244"
     CDS             23177..23668
                     /gene="rocS"
                     /locus_tag="ACL8LA_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGS1813003.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            23766..24356
                     /locus_tag="ACL8LA_001245"
     CDS             23766..24356
                     /locus_tag="ACL8LA_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGS1813004.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            24365..26128
                     /locus_tag="ACL8LA_001246"
     CDS             24365..26128
                     /locus_tag="ACL8LA_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGS1813005.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         7992 a         4783 c         5755 g         7759 t
ORIGIN      
        1 cgaaaaatga gacagtagat ggttatcagc ttggaagcga tggtaaatgg cttggaggaa
       61 aaactacaaa tgaaaatgct gcttactatc aagtagtgcc tgttacagcc aatgtttatg
      121 attcagatgg tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg ctagataagg
      181 atagaaaaag tgatgacaag cgcttggcta ttactatttc tggtttgtca ggctatatga
      241 aaacagaaga tttacaagcg ctagatgcta gtaaggactt tatcccttat tatgagagtg
      301 atggccaccg tttttatcac tatgtggctc agaatgctag tatcccagta gcttctcatc
      361 tttctgatat ggaagtaggc aagaaatatt attcggcaga tggcctgcat tttgatggtt
      421 ttaagcttga gaatcccttc cttttcaaag atttaacaga ggctacaaac tacagtgctg
      481 aagaattgga taaggtattt agtttgctaa acattaacaa tagccttttg gagaacaagg
      541 gcgctacttt taaggaagcc gaagaacatt accatatcaa tgccctttat ctccttgccc
      601 atagtgccct agaaagtaac tggggaagaa gtaaaattgc caaagataag aataatttct
      661 ttggcattac agcctatgat acgacccctt acctttctgc taagacattt gatgatgtgg
      721 ataagggaat tttaggtgca accaagtgga ttaaggaaaa ttatatcgat aggggaagaa
      781 ctttccttgg aaacaaggct tctggtatga atgtggaata tgcttcagac ccttattggg
      841 gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa gctaggtggc aaagattagt
      901 actataagtg aatatgattt gagtgaatag taagttaaaa atcctgattt caagtaaaat
      961 caggattttt tcatggatgc aatttttttg gagtctggtg tgacgcggag ggtcttttgt
     1021 cctgtgtaag tgacaaagcc gggttttcca ccagttggtt tattgagttt tttgacttca
     1081 atcatatcta cctgcaccag attcgacagg cgcccttgag agaagtaggc agctaactct
     1141 gctgcgtctg tcttgactgc atcagatggg tcaagatttc ctgagatgac aacatggctt
     1201 ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc gggccatttt aaaggtcaat
     1261 tcctcatttt gaagattgtt tcgtccgaca tagatgatgg ttttgccatc gcttgctaga
     1321 tattgttcta gttttttgcg tttctggatt ttctcccgtt gtcttctgcg gataaaacct
     1381 gtttgaatca attcttcacg gatttcagcg atttcttcca gtccagcttg gttgaggacg
     1441 gtttctacac tttccagata gagaatagtg gctttggttt cttcaatcaa atcagtcaag
     1501 tatttgacag cttctttgag tttctgatac cgtttaaaat agcgttgggc attctggttg
     1561 ggagtcagag ccttatcaag cccaatcatg ataggttggt tggtatagta gttgtctagg
     1621 ataacctggt cttggtcgtt aggcacttgg tggaggaagg ttgtcagcaa ttctcctttt
     1681 tgacgaaatt cttcagcgtt gtctgtcgcc agtaattctt tttcctgttt tttgagtttg
     1741 tgtcggtttt tctgaagttc attttcaaca cgacgaatca gttcactggc ctgctgtttg
     1801 acgcggtcgc gctcagcctt atccttatag taggtgtcca acaaatcaga aagatttgca
     1861 aaaggctctc ccacctgatt tgcaaaagga actggactga aggaagtctc agtcaagcat
     1921 ggcttggttt cctgattgaa aaaatttcgg aaagcggaaa gtttttcact aaccagtatc
     1981 ctttccaatt catttgccgt atcgcgtccc agaccttgaa agaggctttg aagatttttt
     2041 gctgttagtt cttgggtttg caggatttca aagagctttt catccttgat agtaaaagga
     2101 ttgagagatt ctgtacttgg cggagcgata taggtcgatc ctggaagtaa ggtgcggtag
     2161 ctattttgtg aaaagccgac gtgtttgata acttcgagga ttttatgact gcttttatcg
     2221 accagtagaa tattactgtg tttccccata atttcgataa tcaaggtagc ctggatatgg
     2281 tctccaatct cgtttttatt ggaaactgtc atttccacaa tacggtcatt ttccacttgc
     2341 tcaatcgact caatcagggc cccctgcaaa tactttctca aaaccatgat aaaggtagaa
     2401 ggttgggctg gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa aactggatgg
     2461 gcagaaagga gcaggcgatg gctttggcga ttgctgcgga tttgcaagac caactcttgt
     2521 tcaaaaggct gattgatttt ctggatgcga ccattcacta attcgcttcg caattcctca
     2581 actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc tctccttgtg attgtattcc
     2641 atagtattat atcaaaaagg tagaataaaa tcatggaaat gtggtataat aaagccaagt
     2701 aaagagaaac gagaagcaca tgtatattga aatggtagat gaaactggtc aagtttcaaa
     2761 agaaatgttg caacaaaccc aagaaatttt ggaatttgca gccaaaaaat taggaaaaga
     2821 agacaaggag atggcagtca cttttgtgac caatgagcgt agtcataaac ttaatctgga
     2881 gtaccgtgac accgaccgtc cgacagatgt catcagcctt gagtataaac cagaattgga
     2941 aattgccttt gacgaagagg atttgcttga aaatccagaa ttggcagaga tgatgtctga
     3001 gtttgatgcc tatattgggg aattgttcat ctctatcgat aaggctcatg agcaggccga
     3061 agaatatggt cacagctttg agcgtgagat gggcttcttg gcagtacacg gctttttaca
     3121 tattaacggc tatgatcact atactccgga agaagaagcg gagatgttcg gtttacaaga
     3181 agaaattttg acagcctatg gactcacaag acaataaacg aaaatggaaa aatcgtgacc
     3241 tgatatccag tttagaattt gccatcacag ggatttttac tgctatcaag gaagaacgca
     3301 atatgcgaaa acatgcagtg acggctctag tggtcatcct tgcaggtttt ggttttcagg
     3361 tgtcacgaat cgaatggctc tttctcctat tgagcatttt cttggtggta gcctttgaaa
     3421 ttatcaattc tgctattgaa aatgtggtgg atttggccag tcactatcac ttttccatgc
     3481 tggctaaaaa tgccaaggat atggcggctg gcgcggtatt agtggtttct ctttttgcag
     3541 ccttaacagg cgcattgatt tttctcccac gaatctggga tttattattt taaacagtaa
     3601 gaggaaatta tgacttttaa atcaggcttt gtagccattt taggacgtcc caatgttggg
     3661 aagtcaacct ttttaaatca cgttatgggg caaaagattg ccatcatgag tgacaaggcg
     3721 cagacaacgc gcaataaaat catgggaatt tacacgactg ataaggagca aattgtcttt
     3781 atcgacacac cagggattca caaacctaaa acagctctcg gagatttcat ggttgagtct
     3841 gcctacagta cccttcgcga agtggacact gttcttttca tggtgcctgc tgatgaagcg
     3901 cgtggtaagg gggacgatat gattatcgag cgtctcaagg ctgccaaggt tcctgtgatt
     3961 ttggtggtga ataaaatcga taaggtccat ccagaccagc tcttgtctca gattgatgac
     4021 ttccgtaatc aaatggactt taaggaaatt gttccaatct cagcccttca gggaaataac
     4081 gtgtctcgtc tagtggatat tttgagtgaa aatctggatg aaggtttcca atatttcccg
     4141 tctgatcaaa tcacagacca tccagaacgt ttcttggttt cagaaatggt tcgcgagaaa
     4201 gtcttgcact taactcgtga agagattccg cattctgtag cagtagttgt tgactctatg
     4261 aaacgagacg aagagacaga caaggttcac atccgtgcaa ccatcatggt cgagcgcgat
     4321 agccaaaaag ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat cggtagcatg
     4381 gcccgtcgtg atatcgaact catgctagga gacaaggtct tcctagaaac ctgggtcaag
     4441 gtcaagaaaa actggcgcga taaaaagcta gatttggctg actttggcta taatgaaaga
     4501 gaatactaag tagaggtagg ctcatgcctg cttcttgttt ttacagaagg aggacttatg
     4561 cctgaattac ctgaggttga aaccgtttgt cgtggcttag aaaaattgat tataggaaag
     4621 aagatttcga gtatagaaat tcgctacccc aagatgatta agacggattt ggaagagttt
     4681 caaagggaat tgcctagtca gattatcgag tcaatgggac gtcgtggaaa atatttgctt
     4741 ttttatctga cagacaaggt cttgatttcc catttgcgga tggagggcaa gtatttttac
     4801 tatccagacc aaggacctga acgcaagcat gcccatgttt tctttcattt tgaagatggt
     4861 ggcacgcttg tttatgagga tgttcgcaag tttggaacca tggaactctt ggtgcctgac
     4921 cttttagacg cctactttat ttctaaaaaa ttaggtcctg aaccaagcga acaagacttt
     4981 gatttacagg tctttcaatc tgcccttgcc aagtccaaaa agcctatcaa atcccatctc
     5041 ctagaccaga ccttggtagc tggacttggc aatatctatg tggatgaggt tctctggcga
     5101 gctcaggttc atccagctag accttcccag actttgacag cagaagaagc gactgccatt
     5161 catgaccaga ccattgctgt tttgggccag gctgttgaaa aaggtggctc caccattcgg
     5221 acttatacca atgcctttgg ggaagatgga agcatgcagg actttcatca ggtctatgat
     5281 aagactggtc aagaatgtgt acgctgtggt accatcattg agaaaattca actaggtgga
     5341 cgtggaaccc acttttgtcc aaactgtcaa aggagggact gatgggaaaa atcatcggaa
     5401 tcactggggg aattgcctca ggtaagtcaa ctgtgacaaa ttttctaaga cagcaaggct
     5461 ttcaagcagt ggatgccgac gcagtcgtcc accaactaca gaaacctggt ggtcgtctgt
     5521 ttgaggctct agtacagcac tttgggcaag aaatcattct tgaaaacgga gaactcaatc
     5581 gccctctcct agctagtctc atcttttcaa atcctgaaga gcaaaaatgg tctaatcaaa
     5641 ttcaagggga gattatccgt gaggaactgg ctactttgag agaacagttg gctcagacag
     5701 aagagatttt cttcatggat attcccctac tttttgagca ggactacagc gattggtttg
     5761 ctgagacttg gttggtctat gtggaccgag atgcccaagt agaacgctta atgaaaaggg
     5821 accagttgtc caaagatgaa gctgagtctc gtctggcagc ccagtggcct ttagaaaaaa
     5881 agaaagattt ggccagccag gttcttgata ataatggcaa tcagaaccag cttcttaatc
     5941 aagtgcatat ccttcttgag ggaggtaggc aagatgacag agattaactg gaaggataat
     6001 ctgcgtattg cctggtttgg taattttctg acaggagcca gtatttcttt ggttgtacct
     6061 tttatgccca tcttcgtgga aaatctaggt gtagggagtc agcaagtcgc tttttatgca
     6121 ggcttagcaa tttctgtctc tgctatttcc gcggcgctct tttctcctat ttggggtatt
     6181 cttgctgaca aatacggccg aaaacccatg atgattcggg caggtcttgc tatgactatc
     6241 actatgggag gcttggcctt tgtcccaaat atctattggt taatctttct tcgtttacta
     6301 aacggtgtat ttgcaggttt tgttcctaat gcaacggcac tgatagccag tcaggttcca
     6361 aaggagaaat caggctctgc cttaggtact ttgtctacag gcgtagttgc aggtactcta
     6421 actggtccct ttattggtgg ctttattgca gaattatttg gcattcgtac agttttctta
     6481 ctggttggta gttttctatt tttagctgct attttgacta tttgctttat caaggaagat
     6541 tttcaaccag tagccaagga aaaggctatt ccaacaaagg aattatttac ctcggttaaa
     6601 tatccctatc ttttgctcaa tctcttttta accagttttg tcatccaatt ttcagctcaa
     6661 tcgatcggcc ctattttggc tctttatgta cgcgacttag ggcagacaga gaatcttctt
     6721 tttgtctctg gtttgattgt gtccagtatg ggcttttcca gtatgatgag tgcaggagtc
     6781 atgggcaagc taggtgacaa ggtgggcaat catcgtctct tggttgtcgc ccagttttat
     6841 tcagtcatca tctatctcct ctgtgccaat gcctctagcc cccttcaact aggactctat
     6901 cgtttcctct ttggcttggg aaccggtgcc ttgattcccg gggttaatgc cctactcagc
     6961 aaaatgactc ccaaagccgg catttcgagg gtctttgcct ttaatcaggt attcttttat
     7021 ctgggaggtg ttgttggtcc catggcaggt tctgcagtag caggtcaatt tggctaccat
     7081 gctgtctttt atgcgacaag cctttgtgtt gcctttagtt gtctctttaa cctgattcaa
     7141 tttcgaacat tattaaaagt aaaggaaatc tagtgcgagt aaaaattaat ctcaaatgct
     7201 cctcttgtga cagtatcaat tacctaacca gtaaaaattc aaaaacccat ccagacaaga
     7261 ttgaggtttt aaagtattgt cccaaggaaa gaaaagtaac cctacatctt gaatctaagt
     7321 agattttatg gtaaaataat agggatttta aggagtttga tatgtataac ctattattaa
     7381 ccattttatt agtattatct gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa
     7441 accaatccag caatgtattt gatgccagtt caggtgattt gtttgaacgc agtaaagctc
     7501 gcggttttga agctgtaatg cagcgtttga cagggatttt agtctttttc tggctagcca
     7561 ttgccttagc attgacggta ttatcaagta gataagaaaa taatgggcag gactaggtct
     7621 ttgcctcttt ttatttttaa aggatgtttg agaaggtttt acagtaaaag aaaattaaaa
     7681 aatctagaaa gaaaatatga aagatagaat aaaagaatat ttacaagaca agggaaaggt
     7741 gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt ttcgtgagtt
     7801 gattaaaacc ttgtccttaa tggaaagaaa gcaccagatt cgctttgaag aagatggtag
     7861 tctgacatta gaaattaaga aaaaacatga gattaccctc aaggggattt ttcatgccca
     7921 taaaaatggc tttggctttg ttagtctgga aggcgaggag gacgaccttt ttgtagggaa
     7981 aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta agaaagtcgc
     8041 tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag aacacagttt
     8101 gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg ctggctatat
     8161 tcgttcaaaa aatcataaaa tcagtcaacc gatttatgtt aagaaaccag ccctaaaatt
     8221 agaaggaaca gaagttctca aagtctttat cgataaatac ccaagcaaga aacatgattt
     8281 ctttgtcgct agtgtactcg atgtagtggg acactcaacg gatgtcggaa ttgatgttct
     8341 tgaggtcttg gaatcaatgg acattgtatc tgagtttcca gaagctgttg tcaaggaagc
     8401 agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga
     8461 tgaaattacc tttaccattg acggtgcgga tgccaaggac ttagacgatg cagtgcatat
     8521 caaggctctg aaaaatggca atctggagct tggggttcac atcgcagatg tttcctatta
     8581 tgtgaccgag gggtctgccc ttgacaagga agcccttaac cgtgcgactt ctgtttacgt
     8641 gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct gctctctcaa
     8701 tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac atggtcgtgt
     8761 ggtcaactat accattacac aaacagttat caagaccagt tttcgtatga cctatagcga
     8821 tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaatatcata aaattgtacc
     8881 aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg tgaaacgtgg
     8941 agctctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag gtaagcctgt
     9001 tgatatcgtt cttcggcagc gtggcattgc cgagcggatg attgagtctt ttatgttgat
     9061 ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta tctatcgaat
     9121 tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt cgagttttgg
     9181 cttgcgcatt tatgggactg ccagtgagat tagtcaggag gcacttcaag acatcatgcg
     9241 tgctgttgag ggagaacctt atgtagatgt attgtccatg atgcttcttc gctctatgca
     9301 gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg actattatac
     9361 tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta tgattcggga
     9421 ttacggctgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag agattgcgac
     9481 ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg aagccatgaa
     9541 aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg tatcaagtat
     9601 tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga ttcacatcac
     9661 taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg gagaaaaatc
     9721 aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag cggataaaat
     9781 gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg aaaaaggctt
     9841 gaaacagtct agtcgtagtg gcagagggcg tggttcaaat cgtcgttcgg ataagaagga
     9901 agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa aagacaagaa
     9961 gaaaaaagga aagaaacctt tttacaagga agtagctaag aaaggagcca agcatggcaa
    10021 agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat acaatcgtag
    10081 atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta cgagctgctc
    10141 gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg ctgagcaatg
    10201 ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa cgtcgtcgta
    10261 aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa gggacaggaa
    10321 tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt cttttaggac
    10381 ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt gagcaaaatc
    10441 gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg
    10501 aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg cctgaagctg
    10561 ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc ttgaagggag
    10621 ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac ctctttgaag
    10681 caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa gctgccacag
    10741 atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt gctaaaaaat
    10801 acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg aaacaaggga
    10861 aagctttgga tttgggttgt ggtcaggggc gtaattctct ttttctagcc cagcaagatt
    10921 ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa agcattgtgg
    10981 agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct agcattgaac
    11041 aagaatatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg gaccgcattc
    11101 cagctattat tcaaaatatg caggagaaaa ccagtgttgg cggttacaac cttatcgttt
    11161 gtgccatgga cacggaggat tatccttgct cggttaactt cccattcacc tttaaagaag
    11221 gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa aatccaggcc
    11281 atttgcaccg tcgcgatgag aatggcaatc gtattcaact acgctttgcg accttactag
    11341 ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt ttttcttttt
    11401 tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct aggggagaat
    11461 tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca gcttgtggag
    11521 gccagctcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat aaatccttaa
    11581 aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat aaggaatccc
    11641 tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt tcagaactta
    11701 aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag tgtagtccct
    11761 tgcctcagaa agtccttaaa gagcgaagtg agggctatcg tagtcagggt taccaagtac
    11821 tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta cagcaaggtt
    11881 ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag gaaaaacaag
    11941 ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat tatcaaatca
    12001 aggaattttc ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc tataagagac
    12061 aaaaaatatc tcattttaca gtttctgagg acaaggacat ctgtcgctat atccggcaac
    12121 aactttatta tcaaaatctc ttttggatga aagaacaagc agaagcctat caaaagggag
    12181 aaaatatcct gacttatgga ctgaaagaat ggtatccaca aattcgacca atagtgggca
    12241 aatttttcca gattgaacaa gacttgacta gctattatca gcacttttat acctattacc
    12301 aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc agccttttat cagcaatatt
    12361 tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct aatggtatta caaagaaatg
    12421 aaataaatga aaaagataca tgggatctat caacgatcta cccaactgac caggcttggg
    12481 aagaagcctt aaaagattta acagaacaat tggagacagt agcccagtat gaaggccatc
    12541 tcttggatag tgcggataac ctactagaaa tcactgaatt ttctcttgaa atggaacgcc
    12601 agatagagaa gctttacgtt tatgctcata tgaagaatga ccaggataca cgtgaagcta
    12661 agtatcaaga gtactatgcc aaggccatga cactctacag ccagttagac caagcctttt
    12721 cattctatga gcctgaattt atggagatta gcgaaaagca gtatgctgac tttttagaag
    12781 ctcaaccaaa gctgcaggtt tatcaacact attttgacaa gctcttgcaa ggcaaggatc
    12841 acgttctttc acaacgtgaa gaagaattat tggctggagc tggagaaatc tttggttcag
    12901 caagtgaaac cttcgctatc ttggacaatg cggatattgt gttcccttat gtcctagacg
    12961 atgatggtaa agaagttcag ctatctcatg ggacttacac acgtttgatg gagtctaaaa
    13021 aacgtgaggt tcgccgtggt gcctatcaag ctctttatgc tacttacgaa caattccaac
    13081 acacctatgc caaaaccttg caaaccaatg ttaaggtgca aaattaccgt gctaaagttc
    13141 gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca gaaagtgttt
    13201 atgacaattt ggtagcagca gttcgcaagc atttgccact cttacatcgc tatcttgagc
    13261 ttcgttcaaa aatcttgggg atttcagatc tcaagatgta cgatgtctac acaccgcttt
    13321 catctgttga atacagtttt acctaccaag aagccttgaa aaaagcagaa gatgctttgg
    13381 cagtcttggg tgaggattac ttgagccgtg ttaaacgtgc cttcagcgag cgttggattg
    13441 atgtttacga aaatcaaggc aagcgttcag gtgcctactc tggtggttct tacgatacca
    13501 atgcctttat gcttctcaac tggcaggaca atctggacaa tctctttact cttgttcatg
    13561 aaacaggtca cagtatgcat tcaagctata ctcgtgaaac tcagccttat gtttacgggg
    13621 attactctat ctttttggct gagattgcct caactaccaa tgaaaatatc ttgacggaga
    13681 aattattgga agaagtggaa gacgacgcaa cacgctttgc tattctcaat aacttcctag
    13741 atggtttccg tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa cacgccattc
    13801 accaagcaga tcaaaatggg gaggtcttga caagcgattt cctaaataaa ctctacgcag
    13861 acttgaacca agagtattat ggtttgagta aggaagacaa tcctgaaatc caatacgagt
    13921 gggctcgcat tccacacttc tactataact actatgtata tcaatattca actggctttg
    13981 cggccgcctc agccttggct gaaaaaattg tccatggtag tcaagaagac cgtgaccgct
    14041 atatcgacta cctcaaggca ggtaagtcgg actatccact taatgtcatg agaaaagctg
    14101 gtgttgatat ggagaaggaa gactacctca acgatgcctt tgcagtcttt gaacgccgtt
    14161 taaatgagtt tgaagccctt gttgaaaaat taggattggc ataaaatggt tgaatcgtat
    14221 agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat tgtagacttg
    14281 atgcgtcagc gtcaaaagca ggtcacaggt ttcttgaaag aattggaaga ctttgcccgc
    14341 aaggaaaata ttcctattat tccccatgaa acggttgctt atttccgttt tcttatggaa
    14401 accatgcagc ctaaaaatat tctggaaatt gggacggcta tcggtttttc agctctcttg
    14461 atggctgaac atgcgccaaa tgctaagatt acaactattg atcgtaatcc agaaatgatt
    14521 ggttttgcca aggaaaattt tgcccagttt gacagtcgca agcaaatcac tctcctagag
    14581 ggagatgcgg tggatgtctt atctacactg acagagtctt atgatttcgt atttatggat
    14641 tctgccaagt ctaaatacat cgtctttctg ccagaaatcc tcaaacattt ggaagttggt
    14701 ggtgtggttg tcttggatga tatttttcaa ggtggtgatg ttgccaagga tattatggaa
    14761 gtccgtcgtg gtcagcgaac catttatcga ggccttcaaa aattatttga tgcaacctta
    14821 gacaatccag aactcaccgc aacattagtg cctttaggag atggtattct catgcttcgt
    14881 aaaaatgtag cagatgttca actgtctgaa agcgaatgat tttcagaaaa atttaagaaa
    14941 aaatagtaaa atagatagag taacacttat ctcaaaggag tagacatgaa gaaaaaatta
    15001 ttggcaggtg ccatcacact attatcagta gcaactttag cagcttgttc gaaagggtca
    15061 gaaggagcag accttatcag catgaaaggg gatgtcatca cagaacatca attttatgag
    15121 caagtgaaaa acaacccttc agcccaacaa gtcttgttaa atatgaccat ccaaaaagtt
    15181 tttgaaaaac aatatggctc agagcttgat gataaagagg ttgatgatac tattgccgaa
    15241 gaaaaaaaac aatatggcga aaactaccaa cgtgtcttgt cacaagcagg tatgactctt
    15301 gaaacacgta aagctcaaat tcgtacaagt aaattagttg agttggcagt taagaaggta
    15361 gcagaagctg aattgacaga tgaagcctat aagaaagcct ttgatgagta cactccagat
    15421 gtaacggctc aaatcatccg tcttaataat gaagataagg ccaaagaagt tctcgaaaaa
    15481 gccaaggcag aaggtgctga ttttgctcaa ttagccaaag ataattcaac tgatgaaaaa
    15541 acaaaagaaa atggtggaga aattaccttt gattctgctt caacagaagt acctgagcaa
    15601 gtcaaaaaag ccgctttcgc tttagatgtg gatggtgttt ctgatgtgat tacagcaact
    15661 ggcacacaag cctacagtag ccaatattac attgtaaaac tcactaagaa aacagaaaaa
    15721 tcatctaata ttgatgacta caaagaaaaa ttaaaaactg ttatcttgac tcaaaaacaa
    15781 aatgattcaa catttgttca aagcattatc ggaaaagaat tgcaagcagc caatatcaag
    15841 gttaaggacc aagccttcca aaatatcttt acccaatata tcggtggtgg agattcaagc
    15901 tcaagcagta gtacatcaaa cgaatagtcc aaatcaatga gtcagggaaa aaactctact
    15961 tcaggaaaaa atgaagcaaa cattcccaca ataaaacgcg tagtacaagg tttgtactga
    16021 cccccaaaag ttagacaaat aatttatccg aaggatttag ttctgtattg cacagggcta
    16081 agtcctttta gttttacctt aattctcttg ttgttgtaat aatcaatata gtatataatg
    16141 gcttgttcca attgattaag tgatttaaat gttttctcat agccataaaa catttcggat
    16201 tttaaaatac caaagaaaga ttccatccta ccgttgtctt ggctgtttcc cttgcgtgac
    16261 atagatgctt gaattccctt actctctagg aaccgatgat aagaatcgtg ttggtattgc
    16321 cagtcttggt cactatggag aatcgtattc tcgtagtgct tctctttgaa tgcctgttcc
    16381 aacattaacg atcaatcaat ttaatcatgt acctaatatt agaattgttt atctcaaatt
    16441 tatttgaaag cttctctaag ctatatcctt gttttctaag ttcatagatc tgaactttat
    16501 catcataagt taatttcata ataaaaacac tccaaaagtt agattttttc tgtctaactt
    16561 ttggggtgca gttcatttaa cacctgataa tatgcgtttt ttttatttta aagacttttt
    16621 gaccagcctc attttttaac ttgatactca gtgaaaagca aagattaaac taggaagcta
    16681 gctgtaggct gctcaaagaa cagctttgag gttgcagata aaacttgtga ggtaaccaac
    16741 atatataatg tgaagctgac gtggtttgaa tagattttag aagggtatga gtctggaagt
    16801 tttaatggat aatgcaagat tccatagaat gggtaagcta gagttcttat gtgaagagtt
    16861 tgggcataaa cttttacctt ttcctcccta ctcatcttag tatagaaaag tgaatctaaa
    16921 atagtacata actgcttcta aaacattctt ataaattgat ttaaattctc aaatcatatt
    16981 attcagttct tatttcattt tgctctacaa tcctgttgag aagacacgtg ttcatatcaa
    17041 aaaaggtatt ggcaagttgc aatacctttt tacgaggctc ttttatctta tttttgtttc
    17101 aactgactat atctcctatg gttctagttc agaaggctag gctataatta tgattgataa
    17161 gaagtatcat tccaagtatt gagagtgaat gtttcaaaat catgggtttc tataatggtc
    17221 aggctggcat ttgctagacc gccatcttta cgaagaagtg gttctttata gcctaggaga
    17281 gtacgaagac tggcagtaag attggcgccg tgtccgacaa ttagaatacg ttcagctgga
    17341 ctatctttta atgatttgat aaattggatg gtccgttgag ttgtactata gagggattcg
    17401 gctccgaaca ttcgagtgtc aaattgagca agatttgaac gaaaagcctg gatttgttgc
    17461 gggtaaatag cttccaaggt tgcaattttc aaaccttcta acttcccaag ttgccattca
    17521 cggagattag gaacgatttc taaagaacag ggggtataga gttgactttg gataatctca
    17581 gcagatttga ccgctcgagg taaatcactt gaataaatct gatcaaaagg aatttccttg
    17641 agatactgac caagtcgttt tagggtttca atggattcag gaagaagagg agaatcacca
    17701 ctagcacctt gaaaacgacc ttcttggttc cagagggtac gaccgtggcg gacaaagtag
    17761 agtttcatta cttgatgtcc tccaaaatat ctacaaagtc tgcctttaca aagctagcca
    17821 agtcttgtgg cgcgacgata atgctgtgtc cgacttcgcc tgcagagaca atcatttgat
    17881 ccaaatctag agcaatttta tcgataaaaa tgggataatt gtgtttctga cgaattccga
    17941 caggattatt ggctccatga atgtaaccag ttgttttttc taagtccttt tgtggaatca
    18001 tgctcacttt tttattgcca gaaattttag ctagtttctt ttcagacaag tgctgagtga
    18061 tagggacaat tccgataatc ggtccggtct tgtctcccaa aagcgccaag gttttgaaaa
    18121 tctgatctcg ttcataacct tgaggaagct ctccttctag ggcattgatt tgaatcccct
    18181 gatgagggat agctgcttta gataggattt gttccgccaa tgtttttttg attttaactt
    18241 tttttgccat tatttatatt tatcctccaa ttgactcatc caaataccaa gccagattcc
    18301 cagcgcaaag aagaaggcga tgatgacata accgacaagt gaaagtcctg tgtattggat
    18361 actttcagcg tttcctgcat ttggaattaa gatcaaaagg gtacttgata ggacgatacc
    18421 gatgatgaaa tgatagacac gtgagtggta gttgtttaag gcataatcca tcaattttga
    18481 aaaaacgatg agagttgcac ctgcaccaat tccaatcgga aagaaggttc ccaagaaatc
    18541 aaaagtttta aaaccagtca acataggagc atagagtccc aaaatcaaaa gtaaatttga
    18601 tgggctgagg ccaggaacca agacgccaag ggccaatagt gcgcctgcta ggacgaagtt
    18661 aagaaagctg gcgcttaagg ttccaacgac aaaatttaag gcatagagtc ctaatccaga
    18721 aatgataaag gttgtccata accaagctaa atcaatcttg tctcggtcag attctcgagt
    18781 tgattctttg aggaggctag gaactgtacc gataatagct cccgcaaagc tccataatac
    18841 aaaaacctga taattttcaa gcaggtattc aatcgggtag gaaaataagc cgattcccag
    18901 aagcataccg atggcaactg gaataaagta caaaacattt tctttaaagt ctttaaaggg
    18961 atgggccaga aagccaatca ttcgttcata gattcctaag attgctgcta gaacccctcc
    19021 ggaaattccc ggtaggataa atccaagagc aattacaatc cctttaataa cgcgtgctaa
    19081 ccatgagagc atatatttcc tccaactatt tctatttcaa aaaatcctta ctatattgta
    19141 tcacaatcag acacaaaaag aaaaagcaaa tgataaacaa atgcttttaa ggttttaaaa
    19201 aaagttttcg aaaggtttct tctttatttt ttaagggaga gataacgttg atatctaaat
    19261 cgtggtcaaa gccggcaatt tttcctttag atgtgtattg gtgaatatca taatctaaat
    19321 cagttttagg actgctctcc aaaaatcctg agtctgagcc gtaggacgga atccaaacag
    19381 aggtaaactt gcctgtatca atactgtgtt cttccatgaa gtagacacca acgtagatgc
    19441 cgatgttttt agcacctagt gatgctagtt ttgctcgaaa gttttcgaca ccttcgttca
    19501 tattagacat ggttttgtct tccacgtcaa gccaatagta actagggctg taaggagagg
    19561 cagcattgta gaaaacttcg gcagcctttt ccatttcttg gacacttttt ccagctacat
    19621 aagcgtagac agcaactggg acattccgct tttgaagttc agtgatatga ctcttatagg
    19681 ccttgtctat tccattgata aatgaagcat cattttcttt tgtcgtttga gcaccactgt
    19741 gaacacgaac aatagcacct gaaatatttt gtgagagggc atcgtagttg atttcttcag
    19801 gacgctgcca gccagagagg tcaataatcg gtttgtctaa gtgtttcaaa gcctgtgctt
    19861 caatctgtgc tatattggat tttgttttaa acgattggtt gtcattaagt gggcgattga
    19921 tgattaaaat gaacatcata atccccaaaa aactaaataa aataagtgga tgaatttgtt
    19981 ttctcatatc ttataattct accctaaaaa tcaaaaaaaa tcaaaaaaat gggttaagga
    20041 agagacttta gagcattttt tcattcaaga gtgcggaatg atttgaaata tggtataata
    20101 aaagggaatt tctacagaaa agagaagatt atgtcaaatt ttgccattat tttagcagcg
    20161 ggtaaaggga ctcgcatgaa atctgatttg ccaaaagttt tgcacaaggt tgcgggtatt
    20221 tctatgttgg aacatgtttt ccgtagtgtg ggagctatcc aacctgaaaa gacagtaaca
    20281 gttgtaggac acaaggcaga attggttgag gaggtcttgg ctggacagac agaatttgtg
    20341 actcaatctg aacagttggg aactggtcat gcagttatga tgacagaacc tatcttagaa
    20401 ggtttgtcag gacacacctt ggtcattgca ggagatactc ctttaatcac tggtgaaagc
    20461 ttgaaaaact tgattgattt ccatatcaat cataaaaatg tggccactat cttgactgct
    20521 gaaacggata atccttttgg ctatggacga attgttcgta atgacaatgc tgaggttctt
    20581 cgtattgttg agcagaagga tgctacagat tttgaaaagc aaatcaagga aatcaacact
    20641 ggaacatacg tctttgacaa cgagcgtttg tttgaggctt tgaaaaatat caataccaat
    20701 aacgctcaag gcgaatacta tattacagac gtcattggta ttttccgtga aactggtgaa
    20761 aaagttggcg cttatacttt gaaagatttt gatgaaagtc ttggggtaaa tgaccgtgtg
    20821 gcgcttgcga cagctgagtc agttatgcgt cgtcgcatca atcataaaca tatggtcaac
    20881 ggtgttagct ttgtcaatcc agaagcaact tatatcgata ttgatgttga gattgctccg
    20941 gaagttcaaa tcgaagccaa tgttaccttg aaagggcaaa cgaaaattgg tgctgagact
    21001 gttttgacaa acggtactta tgtagtggac agcactatcg gagcaggagc ggtcattacc
    21061 aattctatga ttgaggaaag tagtgttgca gacggtgtga cagtcggtcc ttatgctcac
    21121 attcgtccaa attcaagtct gggtgcccaa gttcatattg gtaactttgt tgaggtgaaa
    21181 ggatcttcaa tcggtgagaa taccaaggct ggtcatttga cttatatcgg aaactgtgaa
    21241 gtgggaagca acgttaattt cggtgctgga actattacag tcaattatga cggcaaaaac
    21301 aaatacaaga cagtcattgg agacaatgtc tttgttggtt caaattcaac cattattgca
    21361 ccagtagaac ttggtgacaa ttccctcgtt ggtgctggtt caactattac taaagacgtg
    21421 ccagcagatg ctattgctat tggtcgcggt cgtcagatca ataaagacga atatgcaaca
    21481 cgtcttcctc atcatcctaa gaaccagtag gagcctatca tggagtttga agaaaaaacg
    21541 cttagccgaa aagaaatcta tcaaggacca atatttaaac tggtccaaga tcaggttgaa
    21601 ttaccagaag gcaagggaac tgcccaacgg gatttgattt tccacaatgg ggctgtctgt
    21661 gttttagcag taacggatga acaaaaactg atcttggtca agcagtaccg caaagctatc
    21721 gaggctgtct cttacgaaat tccagccgga aaattggaag taggagaaaa cacagcccct
    21781 gtggcagctg cccttcgtga attagaggaa gaaacagcct atacagggaa attagaactc
    21841 ttgtacgatt tttattcagc tattggcttt tgtaatgaga agttaaaact atatttagca
    21901 agcgatttga caaaagtgga aaatccgcgt ccgcaggatg aggatgaaac cttggaagtc
    21961 cttgaagtga gcttagaaga agcgaaagaa ttaatccaat caggtcatat ttgtgatgcc
    22021 aagacaatta tggctgttca gtattgggag ttgcagaaaa aatagaggag gtcagtatgg
    22081 gtaaatcttt attaacggat gaaatgattg aaagagctaa tagaggcgaa aaaatttcag
    22141 gtcctccttt gctagatgat aatgaggaga ctaagatttt accaacctct tcttcccgtt
    22201 ttggttatgc caatcctaag aatcatggtt ttagccagga aaccttgaag attcaggtcg
    22261 aaccatctat tcataaaagc cgtcgcattg aaaataccaa gagaaatgtc ttcaattcta
    22321 agttgaataa aatcttattc gcggtcatct ttctcttgat tttgcttgtt ttagcaatga
    22381 aacttttgta atagaaaagg aattgaaatg aaaataggaa ttattgctgc tatgccagaa
    22441 gaactggctt atctggtcca gcatttatat aatgtccagg agcaagttgt tttggggaat
    22501 acctatcata caggaaccat tgcttctcat gaagtcgttc ttgtagaaag tggaattggt
    22561 aaggtcatgt ctgctatgag tgtggcgatt ttggctgatc atttccaggt ggatgccctt
    22621 attaatacgg gttcagctgg ggcagtagca gaaggtatcg ctgttgggga tgtcgtgatt
    22681 gctgacaaat tagcctatca tgacgtggat gtcacagctt ttggctatgc ttatggacaa
    22741 atggcgcaac aaccgcttta tttcgaatca gacaaaacct ttgttgctca aatccaagag
    22801 agtttatctc aattggacca aaactggcat cttggtttga ttgctacagg agatagtttt
    22861 gttgcaggaa atgacaagat agaagcgatt aagtcccatt tcccagaagt tttagccgtg
    22921 gagatggagg gggcagctat tgctcaagca gcgcataccc tcaatctccc agtcttagtc
    22981 atccgagcta tgagtgacaa tgccaaccat gaagcaaaca tcttttttga tgagtttatt
    23041 atcgaagctg gacgtcgctc tgcccaagtc ttgttggcct ttttgaaggc tttagattaa
    23101 gcggaaattt gacagttttt ctagcttatg ataagattta agtaaagaaa agctagaaaa
    23161 cgtttcagag gatattatga gtattgaaat gaccgtcagt gagattgcag aggtcttagg
    23221 attatctcgc caagcaatca ataaccgtgt caaagaatta ccagaagaag acacagataa
    23281 aaatgacaaa ggggtaacag ttgttaccag aagtggcttg attaagctag aagaaatcta
    23341 taaaaaaacg atttttgaag atgaacctgt cagtgaagat gtcaaacaac gtgaactgat
    23401 ggagattcta gtggatgaga agaatgcaga aattcttcgt ctttatgaac aattaaaagc
    23461 caaggatcgt cagttatcag aaaaagacga gcagatgcgt atcaaagacc gtcagattgc
    23521 tgagaaagac aaacaattag atcagcaaca acaattgacc cttcaagcca tgaaggatca
    23581 agaaaatctt aagctagagc tggaccaagc aaaagaagaa gtccaatcca ctaaaaaagg
    23641 cttttttgct cgtttatttg gaggataatc aaggagctgt ttaggttaaa tgctaacctg
    23701 atggcttctt ttgtttgtga atagtatagt tgattagaac agagacagta gaggagaggt
    23761 acagaatgga acgattagaa gattacattg cttttgattt agaatttaat cagcacgaag
    23821 gacaaacaca cttgattcaa gtatcggcag tgcgttttag agatggtcgg gaaatcgatg
    23881 cctatgattc ctatgttcat accagcgtcc ctctaaaaag ctttatcaat ggtttgaccg
    23941 ggattacagc tgagacctta aaagatgctc ctccagttga gaaagtctta gaagaattcc
    24001 aagagtttgt gggttctttg ctgatgatag gctacaatgc tgctaaaagt gatttaccta
    24061 ttttagcaga gcatggttta gactatagcc aacagtacaa ggtggatttg tatgatgaag
    24121 cttttgaacg tcggagttca gatttacatg gtattgccaa tctcaaattg caaactgttg
    24181 cctcatttct tggatttcaa ggtcagtctc ataatagttt agaggatgct cggatgacgg
    24241 cgcgtgttta cgaatccttc ttagagtcgg atcaagctag ggaattgtta gagacagaaa
    24301 gtagcttatc aaacaatcct tttggtggct tggacttgtc tcaattattt gactaggagt
    24361 gcctatgaaa cctgaaaaac ctaaaaaatt gggtcttaaa cagatttacc tcaatcttga
    24421 taaaatcctc tttctatttt tcttgatttt tatgatagtg gagtatttgt gggtaccatt
    24481 caattcatgg ttggctagct tcttattaga tcagacaggt tatcttttta tttcctataa
    24541 caatgtctta gccatcctta ctcattctcc attgattagt ctagcttttt tagccttgat
    24601 tgtaattaac tttctggttg cttattttca aataggactc ctctttattg gtgctcgtca
    24661 tctcctctat aatgaaaagc gaaggctaat tgagtatatc cgcaaggttt tccatgaaag
    24721 ttttgaattt atgaaaaagc taactggcgc taaagccttg tttatctttt tctatatagc
    24781 tatgctgttt ccttttatac ggaaaatgct gaagatttat tatcttaata aaatcgtcat
    24841 tccagagttt atccaagctt atttagaaga taaatattgg atgtggtggc tgagcatcct
    24901 acttctatct cttatctttc tctatgtctc tgtcagattg atgtttgccc ttccgaagat
    24961 tgtatatgat cagctgactg ttcgagaagc agtgatgttt agcttggaga aaaccaagaa
    25021 gagagttact ttttatgctt ggaatttatt ttacatcttg ctcaaagcac atttattatt
    25081 ttatcttcct ttgattcctt tattattggc tcaaactttg gtagatgata tcactcagag
    25141 agagtctctg attcttggta ttttgaattt tgtcgtgatt aaaaatctct attatatggc
    25201 tctgacttat tttttggtta aatttgtttc atttttgaca ggaaaggagc tggatatgct
    25261 tcctaggaga gaaaaagatc acatcgtgcg atggggtgtc atgacttgtg ccagtttttt
    25321 ctttgcctta gaaggttata tttatctgga ggctcccatg gttcatctac ctcaacttat
    25381 ttctcaccga ggggtttcca atgcaaatgg gattcaaaat acagtagagt ccttggaaac
    25441 tacagcacaa ctcaaaccag acttggtgga gacggacgtg caggaaacaa aagatgggca
    25501 gtttgtcatg atgcatgatg ctaacttgaa aaatctagca ggtatcaata aaagtcctca
    25561 agacttaaac ttggaggagc ttaaagggat tgatattttt gaaaatggct accagactaa
    25621 aatttcaagc tttgaagatt atctcagtcg agccaacgaa cttggtcaaa aattactaat
    25681 tgaaattaaa accagtaaaa aagatagtcc agacatgatg aaccgctttt tagcccgtta
    25741 tgctgcaaag ctcaagattt atggacatca aatccagtct ttagactacc atgttgtcga
    25801 aaaagtaaga cagtatgatg ccgaactgcc agtttatttc atcatgccct acaattctgt
    25861 ctttcctaaa acaagagcga caggatatac tatggagtac tcaaccttgg atgaatattt
    25921 tgtaagcaaa ctatggacaa cggatcagaa actttatgtc tggactgtca atgattcaga
    25981 agctatcagc aaatctcttc acttaggagt agatggagtg attacagatg atttggaaaa
    26041 agttcagcgg gaaatagaag tagcccaaga agatccagaa tatacggatt tgctcttgaa
    26101 aaaagcattg gaattctttg aattttagta ataaaaaaga ggaacggttg ttcctcccat
    26161 gataggtaag tattcttgtg aatgcttgct tttttgtggt tgcaatccgt ttacgagaat
    26221 tgtttgtatt gttcttgttt caatttactt tatagtgctt tgaagttgga atgctacact
    26281 ataacttct
//
LOCUS       DBERYS010000026        25351 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.026, whole genome shotgun sequence.
ACCESSION   DBERYS010000026 DBERYS010000000
VERSION     DBERYS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.026"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            130..741
                     /gene="def"
                     /locus_tag="ACL8LA_001247"
     CDS             130..741
                     /gene="def"
                     /locus_tag="ACL8LA_001247"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS1813006.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            786..1514
                     /gene="rlmB"
                     /locus_tag="ACL8LA_001248"
     CDS             786..1514
                     /gene="rlmB"
                     /locus_tag="ACL8LA_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGS1813007.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(1553..2464)
                     /gene="trxB"
                     /locus_tag="ACL8LA_001249"
     CDS             complement(1553..2464)
                     /gene="trxB"
                     /locus_tag="ACL8LA_001249"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGS1813008.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(2530..2754)
                     /locus_tag="ACL8LA_001250"
     CDS             complement(2530..2754)
                     /locus_tag="ACL8LA_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGS1813009.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(2832..3575)
                     /locus_tag="ACL8LA_001251"
     CDS             complement(2832..3575)
                     /locus_tag="ACL8LA_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1813010.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(3575..4375)
                     /locus_tag="ACL8LA_001252"
     CDS             complement(3575..4375)
                     /locus_tag="ACL8LA_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1813011.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(4533..4889)
                     /locus_tag="ACL8LA_001253"
     CDS             complement(4533..4889)
                     /locus_tag="ACL8LA_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS1813012.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(4902..5414)
                     /locus_tag="ACL8LA_001254"
     CDS             complement(4902..5414)
                     /locus_tag="ACL8LA_001254"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGS1813013.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(5414..5908)
                     /locus_tag="ACL8LA_001255"
     CDS             complement(5414..5908)
                     /locus_tag="ACL8LA_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS1813014.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            6043..6489
                     /locus_tag="ACL8LA_001256"
     CDS             6043..6489
                     /locus_tag="ACL8LA_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGS1813015.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            6486..7133
                     /locus_tag="ACL8LA_001257"
     CDS             6486..7133
                     /locus_tag="ACL8LA_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGS1813016.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(7154..7735)
                     /gene="pdxT"
                     /locus_tag="ACL8LA_001258"
     CDS             complement(7154..7735)
                     /gene="pdxT"
                     /locus_tag="ACL8LA_001258"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGS1813017.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(7736..8611)
                     /gene="pdxS"
                     /locus_tag="ACL8LA_001259"
     CDS             complement(7736..8611)
                     /gene="pdxS"
                     /locus_tag="ACL8LA_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGS1813018.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(8763..10142)
                     /gene="nox"
                     /locus_tag="ACL8LA_001260"
     CDS             complement(8763..10142)
                     /gene="nox"
                     /locus_tag="ACL8LA_001260"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGS1813019.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            10436..11359
                     /locus_tag="ACL8LA_001261"
     CDS             10436..11359
                     /locus_tag="ACL8LA_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGS1813020.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            11419..12024
                     /locus_tag="ACL8LA_001262"
     CDS             11419..12024
                     /locus_tag="ACL8LA_001262"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS1813021.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            12043..13287
                     /locus_tag="ACL8LA_001263"
     CDS             12043..13287
                     /locus_tag="ACL8LA_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGS1813022.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSS
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(13510..13767)
                     /locus_tag="ACL8LA_001264"
     CDS             complement(13510..13767)
                     /locus_tag="ACL8LA_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGS1813023.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(13809..15845)
                     /gene="glyS"
                     /locus_tag="ACL8LA_001265"
     CDS             complement(13809..15845)
                     /gene="glyS"
                     /locus_tag="ACL8LA_001265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGS1813024.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(16105..17022)
                     /gene="glyQ"
                     /locus_tag="ACL8LA_001266"
     CDS             complement(16105..17022)
                     /gene="glyQ"
                     /locus_tag="ACL8LA_001266"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGS1813025.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(17041..17802)
                     /locus_tag="ACL8LA_001267"
     CDS             complement(17041..17802)
                     /locus_tag="ACL8LA_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS1813026.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(18075..18917)
                     /locus_tag="ACL8LA_001268"
     CDS             complement(18075..18917)
                     /locus_tag="ACL8LA_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGS1813027.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLESLKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(19032..20423)
                     /gene="pgdA"
                     /locus_tag="ACL8LA_001269"
     CDS             complement(19032..20423)
                     /gene="pgdA"
                     /locus_tag="ACL8LA_001269"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGS1813028.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(20661..21029)
                     /locus_tag="ACL8LA_001270"
                     /pseudo
     CDS             complement(20661..21029)
                     /locus_tag="ACL8LA_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            21250..22227
                     /locus_tag="ACL8LA_001271"
     CDS             21250..22227
                     /locus_tag="ACL8LA_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1813029.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            22224..23306
                     /locus_tag="ACL8LA_001272"
     CDS             22224..23306
                     /locus_tag="ACL8LA_001272"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1813030.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(23370..24400)
                     /locus_tag="ACL8LA_001273"
                     /pseudo
     CDS             complement(23370..24400)
                     /locus_tag="ACL8LA_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            24843..>25351
                     /locus_tag="ACL8LA_001274"
     CDS             24843..>25351
                     /locus_tag="ACL8LA_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGS1813031.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKNTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDSHFEHDFSRLPEIMSWDVET
                     VRGVTVSIG"
BASE COUNT         7515 a         5637 c         4563 g         7636 t
ORIGIN      
        1 tgctttcaat tcttgtcatt ttgtgatatc atgtagagga aatgaactaa tccacagtgg
       61 cttattccaa gtataccact tgggctttgg cagtagctaa ctgcactaaa cataatataa
      121 ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac
      181 gatattatcc gtgaagggaa tcctactcta cgcacggttg ctgaggaagt cactttcccc
      241 ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa
      301 gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag
      361 ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa
      421 actccacagg aagcctacga tttggaagcc attatgtaca atccaaaaat cgtctctcac
      481 tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg
      541 cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa
      601 aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac
      661 attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat
      721 ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata
      781 gaggcatgaa aacaaatgat attgtctatg gtgtccacgc tgttaccgaa gccctccttg
      841 caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag
      901 tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaatctctct
      961 ctgagattac tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct
     1021 atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca cttctattga
     1081 ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga
     1141 ccaatgtttc aggtgtcatc attcccaagc accgtactgt cggagtaact cctgtcgttg
     1201 ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgacc aacctcagtc
     1261 aaaccttaga taaacttaag gatgaaggtt tctggacctt tggaacggat atgaacggta
     1321 ctccttgcca caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa
     1381 aaggtatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg
     1441 gacatgttca aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc
     1501 gaaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat
     1561 gttctgtaat gaatttatag gcttcttgac cagcgatagc tccatctcca accgctgttg
     1621 ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag
     1681 ttttcatgtg gttatctgtc acaatccatc ctgcctgatc ttggatattc aattctttaa
     1741 caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag gcttgttctg
     1801 tcacttgacc tgttttcaca ttttcaaata cgactgattc tactcggttt tcacccttga
     1861 tttcccttac tacagaatcc cagataaagc tgattttttc attcgcaaag gcgcgatctt
     1921 gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa
     1981 aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact accaataaat
     2041 cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca
     2101 gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg
     2161 tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga
     2221 cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct
     2281 tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg
     2341 tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc
     2401 gagcagcata caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt
     2461 acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc
     2521 acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg
     2581 gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag ccctggctgt
     2641 cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag
     2701 gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc
     2761 tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt
     2821 tatctattat atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct
     2881 tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct
     2941 aagaagagaa ttttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc
     3001 atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct
     3061 gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca
     3121 cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca
     3181 cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca
     3241 gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca
     3301 aataaattaa actgttggaa aaccatagac aacttacggc gtagggcaag gatttcttct
     3361 tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt
     3421 gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc
     3481 aaggctacga cttctccttt ttgaatatcc aagttcagat gatccaagac agtctgtcct
     3541 gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg
     3601 tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgaggcttt
     3661 caattccgat attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc
     3721 gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa
     3781 aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta
     3841 ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac
     3901 tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac
     3961 tagcataagc tgcctcatta aaggcaaaag cgacaatcgc aaaagctgca gctggaatcg
     4021 cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc
     4081 cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga
     4141 aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg
     4201 ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt
     4261 ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg ccatccggaa
     4321 tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa
     4381 catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct
     4441 acggtcagat acagactttg tctacctact agtttatcat actttgaaac agggaagtta
     4501 tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc ttcataagat
     4561 tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg ttttaacaac
     4621 ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct
     4681 tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat
     4741 cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg
     4801 acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa
     4861 catttggggt attcgataaa ttctaacatg tgcttctcct tctatttgtt tatatcttta
     4921 aaatctacgc cttcatgctc caagagccaa gctttctttt ccactcctgc agcatagcct
     4981 gtcagacgct tgcctgctcc caacacacga tgacaaggta ctaggataga ccaaggattg
     5041 cgtcccactg ctccaccaat tgcttgagca gaagccactt gcaggtcttg agcaatttgt
     5101 ccataggtca ctgtctgacc ataaggaatg ccctgtaaat agtcccaaac tctcttttca
     5161 aaattcgttc cgattggcgc caagagcaag ttggataaat cctgaggctt gcctttaaag
     5221 taatcatcta agcaagcaat aactgggtct aaaataggat gactaacaac ttcttctatc
     5281 gtttcatctc ctagtcccct ctcaaaatgc ttctgctcct gaacccaaat tccatacaaa
     5341 taatggtcat cagctacaag tgatagaata ccaattggtg aggagtagag tatttttaca
     5401 tacttctttt gcattatttc tttaattttt gataggccaa gcgttcgcca tctactggca
     5461 ccttaccaac ttgtttaaaa ccaagttttt caaaaatatg ttgcataacc ttgttttcag
     5521 catgcgtatc tgagcgaaaa tcaagataat caaaaccttc aatcaagccc tctaagaagg
     5581 tttgagcaac tccttttccc tgcacatctg ctgccacagc aatacggtga aagactagat
     5641 actctgactc tccagcttgc cagtttccct cataaatagc ttcataggcc tcctctggac
     5701 tcttggtcac agcagcatag gctagcagtt ctccctcttc caaggctacg taggcttgcc
     5761 ctgagatgat atcatcgata ataacatctg catttggata gccattttgc cactggtcac
     5821 taccagcatc tgctaaacat tttttggcat cctccatcac ctgcatgatg gcatctactt
     5881 catttggaaa agctaaacga atttccatct tttttcctca tttctgacta gtttctccca
     5941 tcatagcata atttaagtat ttctacaagc agttaaaact tgactttgtg tttttgttat
     6001 tttataatct agttatcaaa actatataaa aaggagttaa aaatgaaaac taccttttcc
     6061 tacccaaaat gggcagaaat tccaaacatt gacctctatc tggaccaggt tttgctctat
     6121 gtcaatcagg tctgcgcccc tatctctcct aataaagaca agggcctaac agcatctatg
     6181 gtcaacaatt atgtgaaaaa tggttacctg acaaagcctg acaaaaaaaa ataccaacgc
     6241 caacagattg cccgtttgat tgctatcaca accctcaagt ctgtattttc tattcaagaa
     6301 atagcccaga cacttaatac tctacaaact caagcaagtt cagaccaact ctacgatgct
     6361 tttgtggact acatgaacca aggaattgac ccagctaacc ctattatcca aaccagctgc
     6421 caaaccgtta aactctatca tcaaactcta gacttaatcg atcatactca agaggaggta
     6481 atccaatgaa cactagtctt aaactcagca aacaactcag ttttggagag gagattgcta
     6541 atagcgtgac ccatgctgtg ggtgcagtca tcatgcttat cttgctgcct atttcatcca
     6601 tctatagtta tgaagcacac ggatttttat cctctatcgg cgtttccatt ttcgtcatca
     6661 gtctctttct catgttccta tcatccacca tttatcactc tatggcctat ggttcgaccc
     6721 acaaatatgt tttgcgaatc attgaccatt ctatgattta cgttgccatt gccggatcat
     6781 acacgcccgt tgtcttgacc ttgatgaata actggtttgg ctatctgatt attgtcatcc
     6841 aatggggaac gaccatcttt ggtattctct ataaaatctt tgctaaaaag gtcaatgaga
     6901 aatttagcct tgctctttac ctgattatgg gctggttggt tctggctatc attcctgcca
     6961 ttatcagtca aacgacaccc gttttctgga gtctcatggt aactggcgga ctctgttata
     7021 cagttggagc tggattttat gccaagaaaa aaccttattt ccacatgatt tggcatctct
     7081 ttatcctagc tgcgtccgca cttcaataca tcgctattgt ttattacatg taaaaaagtt
     7141 gagaaattca atctcaactt ttttctttac acatattgat aaagtactgg tgcaagcgca
     7201 catcatcagt caattctgga tgaaaagaac ttaccaacat atttttttct tgggctgcaa
     7261 caatttgatt gttcactgtt gctaaaattt ctacaccctc accaacacta ctgataatcg
     7321 gaccacggat aaaggtcatt ggaatcttgc caactccctt acattctgct tccgtgtaga
     7381 aacttcctaa ttggcgccca taagcattac gctcgaccac catatccata gttcctagat
     7441 gactctcttt ctgagaagtg atttccttag ccagcaaaat taagcccgca caggtcccaa
     7501 acactggtaa gccagataga atggcttctc ggatgggaag tagcatgttc tggtcacgta
     7561 agagcttgcc catggttgta gactcaccac caggcaaaat caaacccgac aagtcactct
     7621 gatcttgctg aaaatcatct agatttctga gttctacact ctcgacacct aattgatcta
     7681 gcacttttgc atgttctgca aaggcccctt gcaaggccaa tattccgatt ttcatctatt
     7741 ttcctcgttc agccatgaga atttggattt cattttcatt aataccaacc atggcttctc
     7801 ctaaatcttc agagatttga gctaggattt gaggattacg gaagttagtc acagccttaa
     7861 caatggcact cgctcgttta acaggatctc ctgacttgaa aatacctgaa ccgacaaaga
     7921 ccccctctgc ccctaattgc atcattaacg cagcatctgc tggcgttgca acacctccag
     7981 cagcgaaatt tacaactggc aattttccat gttcatgaac atattggacc aattctacag
     8041 ggacttgcaa atccttggca gcaacataaa gctcgtcctc acgtaagttt tgaatgcggc
     8101 gaatttcctg attcatcata cgcatatgac gaacagcttg gacgatatcc cctgtccctg
     8161 gttctccttt ggtacgaatc atggaagcac cttcagcgat acgacgcaag gcttcaccca
     8221 aatccttagc accacagaca aaaggaactt ggaattcttt cttgtccaca tggaaacggt
     8281 catcagctgg agatagaact tcactctcgt cgatataatc aatttcaata gcctctaaaa
     8341 tctgagcttc aacaaaatgc ccgattctga ccttagccat tactggaata ctaaccgctt
     8401 cttggatttc cttaatcatc tttgggtcgc tcatgcggga aactcctcca gctgcacgaa
     8461 tatcagccgg aattcgttcc aaggccatca cagctgccgc accagcagct tctgcgatac
     8521 gagcctgttc aggattctgc acatccataa taacaccacc cttgagcatc tgtgccaagt
     8581 ttttatttag ttcataacga ttttcagtca tttgttttat cctcatcatt tgtattggta
     8641 gctacctttt cattttaagt cagaacagaa tgaagcacaa gataaaaaaa ctataattgt
     8701 atggggacaa attagctaaa aactatctga ccttaactta aggccagata gctcattcat
     8761 ttttattttt cagccgtaag ggcagccatt gtgatgtagt tgtatggttt gttgaagtgt
     8821 ggcaagaaga agaggtctgt caatgccaat ttatcaattg tcacatgctc ttggatagca
     8881 agtgagaaca tgtggattcc catgctaatt gcaatatcat gtgaaaccat ttgggcacca
     8941 agaatttcac ggctatcttt gtcaaagaca atcttaattg ctacttcatg gttgtcatgt
     9001 ttcatgaatt ctggtttttg aagatcgtta aagcctgttt cagttgcgtt gtaaccagca
     9061 gctttcgctt tttcaagagt caaaccagtt gaaaccatgt gaagaccgta gattgagata
     9121 ccatttgaac cttgaacacc gattccttcc aattcatgtc cacaagcatt gtaggcacca
     9181 acgataccag tgcgaacagc atttgaagca agagcgatat agcttgtatc tttacgagca
     9241 ttgtcataaa cagtcgcaca gtcaccaaca gcgtaaacgt ctgggataga tgtttcttgt
     9301 ttcttgtcta caaggaaggc accgttgcgg aagagttcga tcttaccacc tgcaagggct
     9361 gtgtttggac ggaaaccaac tgcaaggata accatatcca cgtcaaagct ttctttgtca
     9421 gtaatcaagc gttcaacttt accgtcacct tcgattgctt taacagtttg acctagagcc
     9481 aagcggatgt tgtgatcttc caagttcttc gccatcattt gtgtgaagtc tttgtcatag
     9541 taaccgttca agacagtatc aacgatatca acaaggacaa cttcttttcc aagacgttca
     9601 aaggcttcag caagttcaac accgatgtaa ccaccaccaa caacggcgat acggtcgagg
     9661 tgttggctct tgtcagaaag tttattgata acttcttcag cattttggta caatttcacg
     9721 aattgtacgt tttcaagagt tgctttaaat tcgcggtttc ctttaacaat ttcaacacct
     9781 tcgattggtg gcaagattgg tgtagaacct gtagcgaaaa tcaatttttc gtatgattct
     9841 ttgtgctctt ttccttcaac ttccgctgta actactttgt tatcatagtc gattgaaaga
     9901 acaggtgagt tcatgtaaac tttagcacct ttagcttcca atttttcttt atcagaatag
     9961 aacaagcctt cagcaccgtc aatttgttca ccaatccaaa gagccattcc acatcctagg
    10021 aaagagatgt tagagttttg gtcaaataca acaatttcgt tctcatttcc aaaattatcc
    10081 aacatggtat tgatacatgc tgtaccagcg tggttagcac cgactacaac gattttactc
    10141 ataaaaattt cctgccttca ttctaaattt accttactag tataccattt accgttagcg
    10201 tttacaaggg gggtactatt atttcagcat tttttatcaa aaataattcc ccatttatcc
    10261 tgttttcaag cctgatgaat tttatgagca tatttcttga ctttttccaa attttgtttg
    10321 tgaaaattct aactttatgg tattataata acatgaaaat gaaatgtaag ggcttcaatt
    10381 tagatgatta tttgatatta caacttattt tctataagag aaaggagttg gtagcttgac
    10441 tcttcgttca cattctgaac ggctaatggg gactactatc actatttcat tagtggatga
    10501 gcaagccgat atctttctcc aaaaatcctt tgacttgctc aaagaacttg aataccgctt
    10561 caacgccaat agtcaagaat ctgagttgat ggaaatcaat tatcaagcag gaatagcccc
    10621 cgtcacggtt catccagacc tgtttgagct gatttcactt gggttagagc atagcctagc
    10681 gctctctagc cacctcaata tcagcattgg tcccttaatt caaacctggc gtatcggttt
    10741 ttcagatgcc aaggtcgccc agcctcaaga aattgaatcg gtgctgcctc taatcaatcc
    10801 tcatggtatc gagttagatt cttctacttc tactgtgttt ttaaaacaga aaggaatgaa
    10861 gatcgatcta ggttgtttag ccaaaggata cagtgcggat aaggttgccc aatttcttag
    10921 gaaagagggg gtgacttctg ccttgatcaa tctgggaggg aatatcctga ccattggaaa
    10981 aaatcaggca agaggggata acccatggca aatcgggatt caagacccag ccaatcctag
    11041 gggaaatcat ttaatgacca tccctgttgt caataaatct gtcgtgactt caggcattta
    11101 tgaacgtcac ctgaccgtcg atggacaaga ttaccatcac atttttgaca gtcaaacagg
    11161 atatcctgtt gaaacggaac tagcgagtct aacaatcatc tctgataaat cagtcgatgg
    11221 cgaaatctgg acgactcgac tctttggaga aagacctgct tctatcctct ggcaagtcga
    11281 aagtttggag ggcatcgaag tcatcctcat cgataaagaa ggccacctaa gctgttcttc
    11341 aggaattcct actctataga gacaactgtt tcaatggaat tttaaaattg tattatgtaa
    11401 aaagagaaag gaaatctcat gttaaaactt attgctattg ttggaacaaa ttcaaaacgt
    11461 tctacaaacc gtcaattgct tcaatacatg caaaaacact ttactgacaa agctgaaatt
    11521 gaacttgttg aaatcaaggc cattcctgtc ttcaacaaac cagctgacaa gcaagtacct
    11581 gctgaaatat tggaaattgc tgctaaaatc gaagaggcag atggcgttat tatcggtact
    11641 cctgagtatg atcactctat tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat
    11701 ggtatttacc cacttttgaa caaaccaatc atgattacag gtgcttctta cggtacgctt
    11761 ggttcatctc gtgcccaatt gcaacttcgt caaatcttga atgctcctga aatcaaggca
    11821 aatgttcttc cagatgaatt cttgctctca cactctcttc aagcatttaa cccaagtggc
    11881 gacttggttg accttgatgt tatcaagaaa ttggatgcca tctttgatga cttccgtatc
    11941 tttgtaaaaa tcacagaaaa attacgtaat gcacaagaat tacttcgcaa agatgctgaa
    12001 gactttgact gggaaaattt gtaagatagg agaccaaaaa gaatgaaatt tgttggactt
    12061 gttggatcaa actacgatca atcatataac cgtaagctct tggaatttat ccgtcgctac
    12121 ttcaaactca aatttgaatt agaagttctt gaaattgacg aagttccaat gtttaaccaa
    12181 gatgaaaaat gggatgaaag tttccaatta cgttatttat ataacaaaat tactcgtgca
    12241 gatggtgtta tcattgctac tcctgagcat aaccacacta tctcagcttc actcaaatct
    12301 gtacttgaat ggctttcata cgaagttcat ccatttgaaa acaagcctgt tatgattgtg
    12361 ggtgcatcat actatgacca aggaacatca cgtgctcaag ttcaccttcg taaaatcctt
    12421 gatgctccag gtgttaatgc ctacacgctt ccaggtaacg aattccttct tggtaaagct
    12481 aaggaagctt ttgataacaa cggaaatatc accaacgaag gcactgttaa attccttgaa
    12541 acttgcttag ataactttgt taaatacgta ggagtcgttt cgaaattgaa aaaaccaaaa
    12601 ccaatcgaat cagaagactt ggattgtgga aaaccaatcg ctacaaccat cacagaagtt
    12661 gaccctgacg atccagaatg ggtagaaaaa gttgcagcat tcactggtgc tgtttctggt
    12721 gacacctatg tcaaattgga ccacggtatc ctaacagtta accaaattga tatgttcttg
    12781 aaagctatgc catttgaatt gacatacgct gacgacaaca accaattcct ctactacaac
    12841 aacgctcacc aagatccaga cactatgttc gctaaacgtg taccacctca atcaggtagc
    12901 cgtatgtcga ctgttcatgg ttctcttcca ccagcacgta tgaagaacgt agagtgggtt
    12961 atcggaacac ttcgcaacgg aaaccaagaa tacgtccgta cgatcgttcc aggttctcct
    13021 gcaggtgtta tcaacactca caactaccaa gcaatgtact atcctgatgg atcatacgct
    13081 ggtatcaatg aaatcgtctt taacttccaa ccatggcttg actggtacct aaaagagact
    13141 ggtcaacgtt tggtgggtgg tagcggacca tttgctcctg ctgccggagg tcatggagac
    13201 gccgatgcta cttctagcgc ttctgattca ggggatgctg gaggccacgg tggtgacgca
    13261 gacgctacag ccggtgcaag ttactaatga tagtttttag gaactattat ggttcctttt
    13321 ttagcgaaaa aagactagca aacacaaact gctagtctct ttgttatcgt ttcatctatc
    13381 tttgttaaaa gaaagaatac atatactcaa tgaaaatcaa agagcaaact aggaagctag
    13441 ccgcaggctg tacttaagta cggcaaggtg aagctgacgt ggtttgaatt tgattttcga
    13501 agagtattat tatgaatttg gatcatcaag actacggcca tgtaatcctt tttcacgttg
    13561 tacttggcgt agtttttctg gtgtaacatc gtttccttct tcgtccacaa ttttgattcc
    13621 ttcaatgtgg tgacgaacag cgcggcgata accttcgatg tactcctcac gtagtttggc
    13681 ttgttccact ttttcttctg gtgttaagcc ttctgttttt ttctttttag caagctcatt
    13741 gatacgagca atttttttcg gatccatttc ttctcctttg taataagata aagtccgttt
    13801 atcaaatttt atttagtgtt aatttggtta aaacaagcaa acttagctgc tttcttggtt
    13861 agttgtgaca agattgccaa acgattttga cggacagcct gatcttcagc cattaccata
    13921 gtattttcaa agaaagcatc aatgactggg ctaagcgcaa aaagttgttt caattgctga
    13981 cttgcaggtc ctgataaaat gagtgtttct actgcttctg ccaaagcttt ttcttggtca
    14041 ttctcaaata gtgctgaatc aaccgtagca accccttctg ccttctcggc caggttaaag
    14101 gcacgagaaa gtgattcaac agatggttta aaatcttctt ccttgcttac ttctacgaga
    14161 gcacttgctg cttccaacat atctgccaca acaaagtttg aacctgcaag aactgcttcc
    14221 ttgatatctt ttggagtaga gcccatcatc ttatcaacac gagccttgat aaagtccata
    14281 acctctgctt tattttcata agtcaaactg tcaaatttca atgcataaag gctatcaatc
    14341 agctcatcca tagcaatgtg ccaaccaaag gcatccaaga tacgaaccac accttgagtt
    14401 gcacgacgaa gggcataagg gtcattagaa cctgatggaa tcaatcctac tgagaagaaa
    14461 ctcaaaatcg tatccaattt gtctgcaatg gctagaactg cgccgacctt gctctctgga
    14521 agttctcctt cagctgatgt aggcatgtag tgttcacgaa tagcagctgc caccgctgga
    14581 gtttcaccag caagaagggt gtatttttca cccataattc cttggagttc gtcaaattca
    14641 ccaaccatac ctgtcaacaa gtcaaacttg taaatggctg ctgcacgagc aaggtcaact
    14701 gtttcatcca ctgacaaact agctttttct gccaaaagta cagtgatttg acccgtacga
    14761 atcatgtgtt cacgaaggga accaatcttc tcatggaagg tgacattgtt taatttttca
    14821 acaagatctg aaatcaccaa tttttggtct tcacgccaga agaattctcc gtcttccaag
    14881 cgggctacca agactttttc atttcctttg atgacatttt tcaaacgctc tgcgtttccg
    14941 ttacgaacag aaatgaagtt tggcaagagt tttccatctt gatcacgaac aacaaagtaa
    15001 cgctggtgtt ccttcataga agtcaccaag acttcttctg gaacttcaag gtatttagca
    15061 tcaaaacttc ccatgaaggc agttgggtat tcaaccaaat tcaagacttc attcagcaaa
    15121 tccgcatcaa tttcgatacg tacaccatgt tttgcctcaa tttccttgat ttggtcaaca
    15181 atcatttgct cacgttcaca tggatctgcg attacaaact gcttacgaag gtcttcttca
    15241 tagctcaatg ctgactgaat cttggtttct tgtcccaaaa aacgatggcc acgactcaca
    15301 cgacttccct tgatatcaag gaaatccaag tcaaactctt gctcatccaa gagaacagtt
    15361 aaagtgtgaa cagggcggat gtattcaaag ctatttcccg cccagtgcat gctgacaggg
    15421 aaagtcagtg acttcaagac atccacaatg cctggaacaa tggcttcaac tgcttgacca
    15481 atttcttcct tagtgacata gacatattct tcacccttga tttcacggaa ttcgatatct
    15541 tcaacagtca aacctttccc acggacaaat ccttgagctg ctttggtgaa gtttccatca
    15601 ctatctaagg caattttctt tgctggaccc ttgaaatctt ctgttaaatc agactgtttg
    15661 tctgcaagac cagttacacg aacagccaaa cgacgtggtg ttgagaaagt ttgaatggct
    15721 tcaaaagaca ggcgttttcc cttgaggaag gctgccattt tttcgcctag ttgtttttca
    15781 cttggcgtaa caacataggc tggtaattct tcaagaccga gttctactaa taagtttttt
    15841 gtcatgtttt ttcctttaca ttttcttcaa tcttttttga tatgcacctt aggtactggg
    15901 gacgtcgcta actaactctg tgagtctgta tggaaatcca atgctaaatt tatcaagatt
    15961 tccaacaatc tcatcaaagt ggatttagct gactgatttt tgaacctaag gtttcaaaaa
    16021 tccccacata acagtacagc ggtgcatatc cctctagcaa gtcttcgact tgcctctaac
    16081 gattttagag tacagtatta ctcactattc tgcgtcttct gctaaaagtt taactcgtgt
    16141 ttcttcatcc aaaagtgggt aacctaggcg tttgcgttct gcaacaaagg ttttggctac
    16201 aacacgggcc aagttacgga tacgagcgat atagcctgca cgctctgtta cagatacggc
    16261 accacgcgcg tcaagcagat taaaggtatg tgaacatttg agaacatagt cataggcagg
    16321 gtgtaccaag ccttcttcta atgcacgacc agcttctttt tcaaacttat caaaattttc
    16381 aagcaacatt tcttggtccg aaatttcaaa tgaatatttt gagtgctcat actcaggctg
    16441 gataaagatt tctccgtatt ttacaccatc agcccactcg atatcataga cagagtctac
    16501 ttcttgaatg taagaagcca agcgctccaa accataggta acttccgcag tcacagggct
    16561 agttgccaat ccaccgactt gttggaaata agtgaactga gtgatttcca ttccgtcaag
    16621 ccaaacttcc caaccaagac cagctgaacc agttgatggg ttttcccagt tgtcctcaac
    16681 aaaacgaata tcgtgctcca aaggattgat tcccaatttt tccaaagact caaggtaaag
    16741 ttcttggata tttgatggag aaggcttcat gaccacctgg aattggtggt gttggtagag
    16801 acggttaggg ttttccccat aacgaccgtc agcaggacga cgtgatggct ctacataagc
    16861 tgcattccat ggctcaggtc cgatagcacg aaggaaagtg taaggactca ttgttcccgc
    16921 acctttttca ttatcataag cctgcataag catacaacct tggtcattcc aaaattgttg
    16981 caaagtaagg atgatttcct ggaaagtcaa tttcttagac attgtttact cctttagttt
    17041 ttatatttct ttttcaatta atttctgctg aacaaaccaa tctaacaagg gaaaagtttt
    17101 ttctgtattt gcccataaat gataagaatc taagaccgct tgtacactac ctaaattttc
    17161 taccaactta tctactgtca aaaagctgcg ccagtattca taaaaaatac ttgcatagtt
    17221 cttatcataa gtcgaagaac caaaatcatt caatgaatgc caaccatact tcttctgaaa
    17281 aagttctacg agagattgat tacaaatttt ttccgcttga aattcctctt ctgtcaagaa
    17341 atacttgcga ctaatatatt caaccatccc ctcttcgaac cagatataag agtcataacc
    17401 atcaaaatca tctaaaaaat gctccgacca atgagctaac tcatgtccta caatctgtaa
    17461 gaggaaattt tcagataaag aatgatagtg actttctatt gcttgagttt gctcagaaca
    17521 ctcgtagtcc atcaattgat acaaatacaa ctctttccaa acagctaaat caggcgccat
    17581 aaccattcgt ctattatttg tataggctgg aacagcgctt tccctaataa tctgtgtagc
    17641 agcattaaaa tctgaccaaa taacggcttg cggtaaatca cagactgcaa gttcattctt
    17701 taaaaaagat tgataatctt tcaacttttc agtatttctt actacaaaat cacgaaatgt
    17761 agctagttga ctatcgtctt ttacaagata aaggttgtcc acggctctcc tttctttcac
    17821 acaatagact gaataagcaa aagaaccaca tctgacaatc ctaggttgat ttaacctagg
    17881 ggcgtcagaa acgcggttcc accctaattt attacttcat tgtttttgaa aatactacag
    17941 aaagcgccag ctcattattt tgccctactt ggctcccact gtcccaagct cgcttgaaga
    18001 acgccataag actttctttc ctgctgacta ttatatcaaa aattagaata atgtacaatt
    18061 ctttttatga ttttctagaa atccacatca tcaactcgtg gagcccccga ctgaacagca
    18121 gtcgttttta aggtttctct ctcctcatga ctcagttcaa ttccaaaaca atcaagatta
    18181 gcttgaatac gagaggttgt gacagatttt ggaagtggta aaaatccttc tgccaagctc
    18241 caggccaagg ctatctgagc aaccgatttt ccgtgatttg ctgcgatttc ttggacttgc
    18301 ttgctatcaa acagttctcc ttgtccaaaa ggcccccaag cttccaataa aattcccttt
    18361 tcacgacagt aagctacgac ttcctcttga tacacacctg gcgccaagcg aacttgattg
    18421 accgcaggaa cgatagttgc agtttcaagc aaggcatcca aatgatgggg aagaaaattg
    18481 ctaacgccga tagcacggat tttcccttct tgatagaggt cttccatcgc tctccaaact
    18541 tccgcattgc gagttttcca tgcgtcattt tctctgagcg gttttgggtt cggccaatga
    18601 atcaaataca aatccaaata atccaagccc agtttttcta tagatttttc caaagcttga
    18661 cgagtttgct cataggtttg ctgactattc caaagcttgg tagttacgaa catttcttca
    18721 cgtggaactc cgctatcttt gattgcttga ccaacacttt cttcattctg ataaatcgcc
    18781 gccgtatcaa tatgacgata accagccttc aaggattcta acactgcacg ataggcttct
    18841 tctccatcct tagccttaaa agttccaaat cccaatactg gaatttctac tccattattt
    18901 aattgatagg tattcatatg cttctccttc tatatttatt catcaattat agcataaaac
    18961 attttttaag tagtactatc ttctgtaaaa aataagtctg tcaaatatct aacagaccta
    19021 ttttttcttg cttattcatc acgactatag tacagctcat gagcttttag gcgagtattg
    19081 agcatctctg gtatggtcac aaaggtataa ccttgatttt tcaaatactc aatgaccctt
    19141 ggcaaggcat tgactgtcgg actgtgaata tcatgcatca aaacgataga gccgttagct
    19201 acttgatgct gaatttctgt caaaatagat gcttcatttt tactcttcca gtccagacta
    19261 tccacatccc acatgataaa gctcaaatcc aagctattgc gaatatcatc tgtaatagca
    19321 ccataaggtg gacgcatgag tttagaacta gaacccagca ctttagttag cacatcctca
    19381 gtatcagtaa tctgcttttt agcttcatca agagagagtt gcgagagaat cggatggctc
    19441 caactatggt ttccaacaac atgaccttca gattttatcc tcttcaccaa gtcctcattc
    19501 ccagaaacat ttttcccaag cacaaagaaa gtcgctttaa tatcatattt agctagggtc
    19561 tctaatacct gcggggtcgt tgctggattt ggaccatcat caaaggttag agcgacaact
    19621 ttttgatatt tcttatcaaa gtaagattgg tacaaggccg catctttttc gagtaagtac
    19681 gaagattgga taacatcaaa gaaagcagat actggcaagg ctatctcttc taaattttca
    19741 accacaggac ttggataaag gataatctga ctatccttgt aatcaaaatt ccatgcagac
    19801 aagtcttggt cagagaagtt ttttacaatc tgctcactct ggtcttgctc tattttttta
    19861 tcctctatga aggaggtcaa ctcttttatc agctgttcct tagccttact agcatctgaa
    19921 aacagttggt caagtgtaaa aggttgccca tcttctgtta agtgaacctt tcctagactg
    19981 gtcttttcag tctcttcaac cttaaaagca actaaatcat agatttgttt ggtcacatta
    20041 cgattaacga ctccctttaa acctgactct tcttgctctg tatagtagaa aacaagattg
    20101 tccttacttt ctagcttgtc cttaacatct tgatttatca actccctaac agaggaaatc
    20161 actttctccc cttggagagg ataataggca atcacttcgg cttgcccctg acgaaaatgc
    20221 tccttctgat ttccctcact caattgatca tctttctctt ttttgagcga ttcaatcttt
    20281 tgctcaaaac ttttttgctg gtagatctta aaagcaataa atccgcctaa taggcaaata
    20341 gaaattgcta aaataccaat caaagccaat aatacatgtc tcgttttccc gtgtctgcca
    20401 cgtcctagtc tacttttatt catatttata tcataacaaa tttgagcaat aatttcaatg
    20461 atacaatttg gcaatccaca attcctctag caagccaaag catcttacat caactccaac
    20521 atccgtatct gtagagatta tgtagttttc tatctatgga atttctagta gattgaagct
    20581 agaatagtag acctatactt ctaaaacatt gttagaaatc gatttgattg tcctgatcga
    20641 tttgtcctgt tctcatttca ttttactata aaactatgaa aagctaggtc atctgagaag
    20701 taattactat cgacaattgc gatagcgtat actggtgcga tttgtttgta gctctggtgg
    20761 gtatcacctt cacgttggcg aattttttct aggttttgat taacctgact gcacagataa
    20821 gcccataggc gattgatgaa aaaattctga tgatgaacct gaatttcgat gataacttgg
    20881 attccattat ctaactccgc caagacgtct atactagtat agaaatcctg cgctgagtag
    20941 ggcatggaag gcaagacgtg aatgttactt ccctccaaaa tcgtcacatt ttttgctggc
    21001 aagtccagca tatcgcggat aaattgacaa atcattagtt gggctgatac ccggatgtct
    21061 gagaatcctc cttttcctcc ttctattata gcacattttt aaatctaggt ttgctagatt
    21121 ctatgcttct atctatttat tcggaaaaga aagctaatag cttatttgaa attttctcct
    21181 ctctgttgga tagtcccgtc tatctatgtt ataatgaaag aaggatttaa aatcggaaaa
    21241 ggagtattta tgctaaaatt aggtgtcatc ggaactggcg ctatcagcca tcatttcata
    21301 gaagcagccc ataccagtgg agaataccaa ctggtcgcaa tctattctag aaaactagaa
    21361 actgcagcaa cctttgcttc tcgctatcag aatatccaac tattcgatca attagaagtc
    21421 ttcttcaaat cttcttttga tttagtctat atcgccagtc caaactcctt acattttgct
    21481 caggcaaaag ctgccttgtc tgctggtaaa catgttattc tcgaaaagcc agctgtcact
    21541 caaccacaag aatggtttga tttgattcaa acagctgaaa aaaataactg ttttatcttt
    21601 gaagcagctc gtaattacca cgagaaagcc tttactacta tcaaaaactt tttagcagat
    21661 aagcaagtgt taggagcaga tttcaactat gccaaatact cttccaagat gcctgacttg
    21721 ttgactggac aaacaccaaa tgtcttttca aaccgttttg ctggtggagc cctcatggat
    21781 ttggggattt atcccctcta tgctgctgtt cgtctttttg gaaaagccaa tgacgcgact
    21841 taccatgctc aacagcttga caatagcatt gacctaaatg gagatggtat cctcttctac
    21901 ccagactatc aagttcacat caaggctgga aaaaacatca cttccaatct tccttgcgag
    21961 atttatacaa cagatggaac cttgactctc aacacgattg agcatattcg ctcagctatt
    22021 tttaccgacc accaaggaaa tcaagtccag cttcctatcc aacaggctcc tcatacgatg
    22081 actgaggaag tcgctgcatt tgcacacatg atccagcaac cagatctgaa tctctaccaa
    22141 acttggctgt atgatgcagg ctctgttcat gagctactat ataccatgcg ccagactgct
    22201 ggcattagat ttgaggcaga aaaatgaaaa ccaaactacc gactgaatgg caagaactga
    22261 gtgaccaact cggtttccaa gaattcaccc ccattcaaac tcaactattt gagccccttc
    22321 ttgctggaga aaacctccta ggagtgagcc aaaccggaac tggtaagacc ctagcgtatc
    22381 tcctaccaag tcttctcaga ctacaaaaga aaaaagccca acaactcttg attctagcac
    22441 caaacacgga acttgctgga cagatttttg atgtatgtaa aacgtgggca gaagctatcg
    22501 gattgactgc tcaactcttc ctttcaggtt caagtcagaa acgacaaatt gaacgcctta
    22561 aaaaggggcc agaaattctg attggaactc ctggccgtat ctttgaactc attaaactta
    22621 aaaaaatcaa gatgatgaat gtggaaacca tcatcctgga tgaatttgac caattactag
    22681 atgattctca gattcacttt gtagaaaaaa tcactcacta cgcacctcgt gaccaccaac
    22741 tggtctacat gagtgccacg accaagtttg accaagaaaa gattgtgccg aatacacgca
    22801 ctattaatct ctctgaccaa aaattggaca atatccagca tttctacatg caggtagacc
    22861 aacgtcatcg agtggatatg ctacgaaaac tggctcatgt agaggatttc cgtggtctag
    22921 tcttcttcaa tagcctgtca gaccttggga atgccgagga aaaactacag tatcgtgata
    22981 tattgactgt ttccctcgct agtgatgtta atgttaaatt tagaaaaatc atcttagaaa
    23041 agtttaaaga taaccaacta accctgcttc ttgcaactga ccttttggct cgtggaattg
    23101 atatcgatag cctagaatgt gtcgtaaact ttgatattcc tagagatagc gaaacctaca
    23161 cacaccgtgc tggccgtact ggccgtatgg gcaaggaagg atatgttatt actctcgtca
    23221 ctcatccaga agaaatcaaa aaacttaaaa agtttgcaag tatccgagaa attgtcctaa
    23281 aaaaccaaga actctatatc aaataaagcc ccattgctct atacacatcc agtgtatgga
    23341 gcttttttat aagcaattca attttatact catctgcttt caaaaagcat tctattccat
    23401 ctccgattaa cgatggactt tatcacctcc ttctccagtc cttgtatgac atcttggagt
    23461 tgattcatga catcttccaa agttcgaaag gctttattct taaatccacg tttacgaatc
    23521 tctttccaca cttgttcaat gggttcatct ctggtgtgta tggaggaata aaggtaaaat
    23581 caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga
    23641 gtaaaaaata atcatctgga taagcttgtg aaagctcttc taaaaagacg ttcatccact
    23701 cagtattaca tccaccagct attaagaaaa atgattcgcc tgtatggggc atcaacagct
    23761 ccataacaat agcgaaattc tcgtatatag tgactatgga catgtggacc tactcctatt
    23821 ggagaccaac aagatcccag tttactgatt ctaccgaaac cagcctcatc ttggtacatc
    23881 aagcgaacct tatgaaaagg tctactggtt ttaaagcgct ttcttgtctt cttgaattga
    23941 gattttattt ttagacgcga caatggtttg agcgtctgct ttcctaggat gttctggacg
    24001 tggcataata tttcgccaac catggtgctt caacagttga tagaaggcat cacgtgtgta
    24061 ggaacgatat aagcctgaaa taaggtattg taacaaattc tcctgcctct gtagccttca
    24121 aatggcgggc aagaaaggct ttctcttcct caactgtcat atatgcatgg ttacgaccac
    24181 cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc atattttttt acatttcgcc
    24241 aaatcgttgt ttgattacag tctaaaagat ctataatctc tttataagat ttgcccatca
    24301 gacgaaatag aacgatttga aggcgtttat gatatttagc tgtacgaaag tcttttaaaa
    24361 gtgttttgat ggtttggatt tcttctttag ttgatttcat attactattg tataatgctt
    24421 tttgatttga gtctagtatt atactcttcg aaaatctctt caaaccgcgt cagcttcgcc
    24481 ttgtcgtata gaatgttact gacttcgtca gttctatcta caacctaaaa acagtgtttt
    24541 gagcagtctg cagctaactt tctataacaa aatgaaataa gaacaagaca aatcaatcaa
    24601 gacaatcaaa tcgatttcta acaatgtttt agaagtagat gtgtactatt ccagtttcaa
    24661 tatattatag tttgctcttt gattttcatt gagtattagt tcaatatcta aaaaaagagg
    24721 ctctataata tttgtagtgg gtaaatcccc tatagatatt atggagccta tttttgtgta
    24781 gaaaaaaagt cccatatgac ctataatgaa aagcgacaaa acaactcatt agaaagaatc
    24841 atatggaaca attacatttt atcacaaaat tactagacat taaagaccct aatatccaga
    24901 ttttagacat catcaataag aatacacaca aggaaatcat cgccaaactg gactacgacg
    24961 caccatcttg ccctgagtgc ggaaaccaat tgaagaaata tgactttcaa aaaccgtcta
    25021 agatccctta cctcgaaaca actggtatgc ctactagaat tctccttaga aagcgtcgat
    25081 tcaagtgcta tcactgttca aaaatgatgg tcgctgaaac ttctatcgtc aagaagaatc
    25141 accaaatccc tcgtatcatc aaccaaaaga ttgctcaaaa gttaattgaa aagatttcta
    25201 tgactgatat tgcccatcag ctttccatct caacttcaac tgtcattcgc aagctcaatg
    25261 attctcactt tgagcatgat ttttcgcgtc ttcctgagat tatgtcctgg gacgttgaaa
    25321 cagtccgggg agtgactgtt tcaatcggga g
//
LOCUS       DBERYS010000027        24480 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.027, whole genome shotgun sequence.
ACCESSION   DBERYS010000027 DBERYS010000000
VERSION     DBERYS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.027"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="ACL8LA_001275"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="ACL8LA_001275"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGS1813032.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /gene="yycF"
                     /locus_tag="ACL8LA_001276"
     CDS             1317..2021
                     /gene="yycF"
                     /locus_tag="ACL8LA_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGS1813033.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="ACL8LA_001277"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="ACL8LA_001277"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGS1813034.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="ACL8LA_001278"
     CDS             3365..4174
                     /locus_tag="ACL8LA_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS1813035.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /gene="relB"
                     /locus_tag="ACL8LA_001279"
     CDS             4265..4507
                     /gene="relB"
                     /locus_tag="ACL8LA_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGS1813036.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="ACL8LA_001280"
     CDS             4509..4763
                     /locus_tag="ACL8LA_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS1813037.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..5553
                     /locus_tag="ACL8LA_001281"
                     /pseudo
     CDS             4810..5553
                     /locus_tag="ACL8LA_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(5854..6840)
                     /locus_tag="ACL8LA_001282"
     CDS             complement(5854..6840)
                     /locus_tag="ACL8LA_001282"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGS1813038.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7034..9502
                     /gene="gyrA"
                     /locus_tag="ACL8LA_001283"
     CDS             7034..9502
                     /gene="gyrA"
                     /locus_tag="ACL8LA_001283"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGS1813039.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9502..10245
                     /gene="srtA"
                     /locus_tag="ACL8LA_001284"
     CDS             9502..10245
                     /gene="srtA"
                     /locus_tag="ACL8LA_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGS1813040.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            10489..11286
                     /locus_tag="ACL8LA_001285"
     CDS             10489..11286
                     /locus_tag="ACL8LA_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGS1813041.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            11434..12693
                     /locus_tag="ACL8LA_001286"
                     /pseudo
     CDS             11434..12693
                     /locus_tag="ACL8LA_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            12789..14063
                     /locus_tag="ACL8LA_001287"
     CDS             12789..14063
                     /locus_tag="ACL8LA_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGS1813042.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELCLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            14073..14951
                     /gene="truB"
                     /locus_tag="ACL8LA_001288"
     CDS             14073..14951
                     /gene="truB"
                     /locus_tag="ACL8LA_001288"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGS1813043.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            15192..15494
                     /locus_tag="ACL8LA_001289"
     CDS             15192..15494
                     /locus_tag="ACL8LA_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS1813044.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            15602..16240
                     /gene="udk"
                     /locus_tag="ACL8LA_001290"
     CDS             15602..16240
                     /gene="udk"
                     /locus_tag="ACL8LA_001290"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS1813045.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            16368..17708
                     /gene="xseA"
                     /locus_tag="ACL8LA_001291"
     CDS             16368..17708
                     /gene="xseA"
                     /locus_tag="ACL8LA_001291"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGS1813046.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            17686..17898
                     /locus_tag="ACL8LA_001292"
     CDS             17686..17898
                     /locus_tag="ACL8LA_001292"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGS1813047.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            17895..18770
                     /locus_tag="ACL8LA_001293"
     CDS             17895..18770
                     /locus_tag="ACL8LA_001293"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGS1813048.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            18763..19578
                     /locus_tag="ACL8LA_001294"
     CDS             18763..19578
                     /locus_tag="ACL8LA_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGS1813049.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            19571..20002
                     /locus_tag="ACL8LA_001295"
     CDS             19571..20002
                     /locus_tag="ACL8LA_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1813050.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            20009..21676
                     /gene="recN"
                     /locus_tag="ACL8LA_001296"
     CDS             20009..21676
                     /gene="recN"
                     /locus_tag="ACL8LA_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGS1813051.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            21678..22406
                     /locus_tag="ACL8LA_001297"
     CDS             21678..22406
                     /locus_tag="ACL8LA_001297"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1813052.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            22485..24308
                     /gene="lepA"
                     /locus_tag="ACL8LA_001298"
     CDS             22485..24308
                     /gene="lepA"
                     /locus_tag="ACL8LA_001298"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGS1813053.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         7400 a         4324 c         5666 g         7090 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacgaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tccttttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggctatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaataa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgttttat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaac caatgttttg gaaggtccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt cgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattaaag ctgagtaata acaatataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggttattt gcagattatt atgatacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgac tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttattactaa aagaaaatgg cgctttattc ttgaaaatat ttttgattta
     5041 ttgaccattc tccctttaaa tgctattttt acagtgtttc gattaggacg tatcttccgc
     5101 ttagcaaggc tgacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaactag aaagaaaaat tagtagattc ttacgaacaa atggattgat ttatatcttg
     5221 tatgttaata tctttattgt tctagtaggg agttcgattt tatccgttgt tgaagaaaaa
     5281 tccttctcag atagtctttg gtgggctctt gtgacagtaa ctactgttgg ttatggtgat
     5341 attgttccag tttccctttt tgggaaatgg ctagcagttt tactgatgct tgtcggtatt
     5401 ggaacaatag ggatgctaac aagtgctttg acgaactttt ttgtaaagga taatccagat
     5461 gaacagataa aacttgataa actcaaagac gaattatcga gctaaagaat attaatagag
     5521 aaacaatctg aaaaaatcga agaactacat tgaatgataa aagacttagt tgaaaaaata
     5581 tcataaaggt gagacaaaat aaaacttgat tcgaggagaa atcaagtaag ttttgacaca
     5641 atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag agaaataaaa
     5701 taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtaat
     5761 ggggtgctat ttcaacttca acctactata atacagaaaa aaatacagaa aaaaacaact
     5821 ccctgatgat tcaaggagtt gtctatagtt aaattagttt ttagaagctt cttggaattc
     5881 tggatttttc catgcttcgt caataatagc ttgcaattct ttagcagatg cttgcatttt
     5941 ttgagtttct gcatcgttca atggaatgtt tactggacga acgataccat gtgcaccaac
     6001 aacagctggt tgaccgataa agacattctc aactccgtat tgaccttctt ggaatactga
     6061 aagtggaagt actgcgtttt catcgtcaag gattgcttta gtgatacgag caagggctac
     6121 tgcgataccg tagtatgttg cacctttttt gttgatgatt gtgtaggctg catcacgaac
     6181 accttcgaac aattcaatca attcagcttc ttgaacattt tgagtgtctt taaggaattc
     6241 ttcaaggttt acaccagcga tgttagcgtg tgaccaaaca gcgaactcag agtcaccgtg
     6301 ttcacccatg atgtaggcgt gcactgaacg agcatccaca tccaattttt cagcaagtgc
     6361 ttgacggaaa cgagctgagt caagtgaagt acctgaaccg ataacgcgtt ctttagggaa
     6421 accagagaat ttccaagttg agtaagtcaa aacgtcaact gggttagcag caacaaggaa
     6481 gatacctttg aaaccagatt caacaacttg agttacgatt gatttgttga tagcaaggtt
     6541 tttacctaca aggtcaagac gagtttcacc tggtttttga ggtgcacctg cagtgatcac
     6601 aacaaggtca gcgtctgcac agtcagagta ttgagctgca tagatttttt taggtgaagt
     6661 gaaggcaagg gcgtgactaa ggtcaagcgc atcaccaaca gctttttcat gcaattgtgg
     6721 aatttcgata attccaagct cttgtgcaat tccttggtta acaagtgcaa aagcgtaaga
     6781 tgaacctaca gcaccatcac cgacaaggat aacttttttg tgttgtttag ttgaagtcat
     6841 tgttttaaac atctccttaa ttttattagg ggattttccc tagacaactt cattctatca
     6901 cttttaaaaa actttgtcac gaatatgcct tatagttctc gatgtaaacg ttttagtggt
     6961 ttagaggctg aaatagatgg gaatttatgg tataatgttg ttacttacta attgtgaaat
     7021 gaggcattta ttaatgcagg ataaaaattt agtgaatgtc aatctgacaa aggagatgaa
     7081 ggcaagtttt atcgactacg ccatgagtgt tatcgtagcg cgagctcttc ctgatgttcg
     7141 agatggctta aaacctgttc accgtcgcat tctctacgga atgaatgaat tgggtgtgac
     7201 cccagacaaa ccccataaaa aatctgctcg tattacaggg gatgtcatgg gtaaatatca
     7261 cccacacggg gattcctcta tttatgaagc catggtccgt atggctcaat ggtggagcta
     7321 ccgttacatg cttgtagatg gtcatgggaa ttttggttcc atggatggag atagtgctgc
     7381 cgctcaacgt tataccgagg cacgtatgag caagattgct ctggaaatgc ttcgtgatat
     7441 caacaaaaat acagttgatt tcgttgataa ctatgatgcc aatgaacggg aacccttggt
     7501 cttgccagcg cgttttccaa accttttggt taatggagca actggtatcg cggttgggat
     7561 ggcaaccaat attccacctc ataatctggg tgaaaccatt gatgcagtga agttggtcat
     7621 ggataatcct gaagtgacta ccaaggactt gatggaagtc ttgcctggac cagattttcc
     7681 aactggtgct cttgtcatgg ggaaatcagg tatccataag gcttatgaaa caggtaaagg
     7741 ttcgattgtc ctacgttctc gtacagagat tgaaacgact aagactggtc gtgagcgtat
     7801 cgttgtaaca gaatttcctt acatggtcaa taaaaccaag gtgcatgagc atattgttcg
     7861 cttggttcag gaaaaacgca ttgagggtat cacagcagta cgtgatgagt caaaccgtga
     7921 aggtgttcga tttgttattg aagtcaagcg cgacgcctca gccaatgtta ttctcaataa
     7981 cctcttcaaa atgacccaaa tgcaaaccaa ttttggattc aatatgctcg ctatccaaaa
     8041 tggtataccg aaaattttgt ctcttcgtca gattttggat gcttatattg agcaccaaaa
     8101 agaagtggtt gttcgtcgta cacgttttga taaggaaaaa gcggaagcgc gcgctcatat
     8161 cttagaaggt ctcttgattg cgctagacca tatcgacgaa gtgattcgta tcatccgtgc
     8221 tagtgaaacg gatgcggaag ctcaagctga gttgatgagc aagtttaagc tttctgaacg
     8281 tcaaagtcaa gctatccttg atatgcgtct tcgtcgtttg acaggtttgg aacgcgataa
     8341 gattcaatct gagtatgatg acctcttggc tctgattgcg gatttagcag atattcttgc
     8401 taagcctgaa cgtgtttctc aaattatcaa agacgaattg gatgaagtta aacgtaaatt
     8461 ttctgataaa cgccgtacag agttgatggt tggacagatc ttgagtctcg aggatgagga
     8521 cttgattgaa gaatcggatg tcttgattac cctttctaac agaggctaca ttaagcgttt
     8581 ggatcaggac gagttcactg ctcaaaaacg tgggggtcgt ggtgtccaag gaacgggagt
     8641 gaaagatgat gactttgttc gtgagttagt gtcaactagc acccatgatc atctgctctt
     8701 cttcacaaac aagggacgtg tctatcgtct taaaggttat gaaattcctg agtatggtcg
     8761 gactgccaaa gggctaccag tagtcaatct cttgaaattg gatgaagatg aaagtattca
     8821 gacggttatc aatgttgagt ctgatcgcag tgatgatgct tatctcttct ttacaacccg
     8881 tcacggtatt gtgaagagaa ctagtgttaa ggagtttgcc aatattcgtc aaaatggtct
     8941 caaagcgctg aatttaaagg atgaagatga gttaatcaat gtcttgttga cagaaggaga
     9001 tatggatatt atcattggta ccaagtttgg ttatgcagtt cgctttaatc aatcagccgt
     9061 tcgtggtatg agccgtatcg ccactggtgt gaaaggtgtt aaccttcgtg aaggagacac
     9121 agttgttggt gccagcttga ttactgatca agatgaggtt cttattatca cagaaaaagg
     9181 atatggtaag cgtacagtcg ctactgaata cccaacaaaa ggtcgtggtg gtaagggaat
     9241 gcagacagct aaaattaccg aaaaaaatgg cttgctggcc ggtcttatga ctgttcaagg
     9301 ggatgaggat ttgatgatta tcactgatac aggtgtcatg attcgaacca atcttgccaa
     9361 tatttcacaa acaggacgtg caactatggg agttaaagta atgcgcctgg atcaagatgc
     9421 tcagatagtg actttcacaa cggttgcggt ggcagaaaaa gaagaagttg ggacagaaaa
     9481 cgaaacagaa ggtgaagcat aatgtctcaa aaaaataata aaaagaaaaa caagcgaaaa
     9541 aatctgctga caaatatcct agcaggattt ctgatattac tgtcactggc tttgattttt
     9601 aatactcaaa ttcgaaatat tttcatagtc tggaatacca ataagtatca agttagccag
     9661 gtatcaaaag aaaaattaga agaaaatcag gatacagaag gcaattttga ctttgattct
     9721 gtcaaagcta tctcttcgga agctgttcta acttctcaat gggatgctca aaaattacca
     9781 gttattgggg gaattgcaat tcctgaattg gaaatgaatt tgccgatttt taaaggactt
     9841 gataatgtta atctcttcta cggagctggt acaatgaaac gcgagcaagt aatgggagaa
     9901 ggaaattata gtctagctag tcaccatatc tttggtgttg ataatgctaa taaaatgtta
     9961 ttttctcctt tagataatgc taaaaatggc atgaagattt atctaaccga taaaaataaa
    10021 gtttatactt atgaaatacg tgaagtcaaa cgtgtgacac cggatcgtgt tgatgaagtt
    10081 gatgatagag atggggtcaa tgaaatcaca ttagtaacct gtgaagacct tgctgctaca
    10141 gaacgtatta ttgtcaaagg tgatttgaaa gaaacaaaag attattcaca aacatctgat
    10201 gaaattctaa cagctttcaa tcaaccatat aaacaatttt attaatacaa atcagtgaaa
    10261 tcatggattt cactgatttt ttaagatttt cataaaaata tagtaaaatg aaataagaat
    10321 aggacgaatc gttcaggaca gtcaaatcga tttctaacaa tattttagaa gtagaggtgt
    10381 actattctag tttcaatcta ctataaactt ttatgaaata cagaaaacgc ttcctaaaac
    10441 ctaaagtttg tgatataatt atcaagaaaa aagatttagg agtttattat ggtttcttca
    10501 gaatttatct caaagattga atttgcttgc aataagaaag aaagtcttta tagtcaaagc
    10561 aaatttaagt atgcgattcg ttcgatgttc gcaggtgcat ttttaacctt cagtactgct
    10621 gcaggtgcag ttggggctga cttgattaat aaaattgcac caggtagtgg acgcttcctc
    10681 tttccattcg tttttgcttg gggcttggcc tacattgttt ttttgaatgc cgagttggtc
    10741 acttcaaaca tgatgttctt gactgctggt agtttcttaa aaaaaatctc ttggagaaaa
    10801 acagctgaga ttttactata ctgtaccttg ttcaacctta tcggagcctt gatagcaggg
    10861 tggggctttg ctcattcggc agcctatgcg aatctgatac acgatagttt catctcaggt
    10921 gttgttgaga tgaagttagg ccgctcaaat gaattggtct tgcttgaggc gattttggca
    10981 aatattttcg taaatattgc gattctgtca tttattttgg tcaaagatgg tggtgccaaa
    11041 ctttggcttg tgttgtcagc tatttacatg tttgtattct taacaaacga gcacattgcg
    11101 gcgaactttg cttctttcgc gattgtgaaa ttcagtgttg ctgcggattc aattgccaac
    11161 ttcggtgttg gaaatatgct tcgccactgg ggtgtgactt tcatcggaaa ctttatcgga
    11221 ggaggcctct tgatgggtct tccatatgcc ttcctcaata aaaacgaaga tacttatgta
    11281 gattaagaaa atgagcacga ttgagtcgtg cttttttcat tttcaaaata aggtaatagc
    11341 tatttcttat atcaaaatat agaaaactga tatttgtaaa ctataactca aggtgctaca
    11401 atatccttaa taaaatgata tggaggtcac cttatgactt gtgattttaa atctgaaact
    11461 ctacaactac atgctggtca agttgtggct ccagctacta agtctcgtgc agtgccgatt
    11521 tatcaaacaa cattttttgt ttttgatgac acgtaggaag gtgccgatct gtttgccttg
    11581 aggaaaccag ggaacattta tactcgtatc accaatccta caacagctgc ccttgaaggt
    11641 ggtgttgaag cgctagcaac agcatcaggt atgactgcag tgacttatac gattttggcg
    11701 cttgcccatg ctggtgacca tgtagtggct gcttcgacta tttacggtgg aaccttcaat
    11761 cttttgaaag aaccccttcc tcgttatggt atcacaacaa cctttgtcga tgttgataat
    11821 ttggaggaag tagaagcagc tatcaaagac aataccaagc ttgtcttgat tgaaaccttg
    11881 ggtaactcct tgattaatat tccagacctg gaaaaactgg cagagattgc tcataaacat
    11941 caaatcccac ttgtgtcaga caatactttt gcaatacctt atttgattaa cgtcttctct
    12001 catggcgttg acattgccat tcactctgcg actaagttta tcggtgggca tggtacaact
    12061 attggaggaa taattgtcga tagtggtcgt tttgactgga tggcttcagg gaaattccct
    12121 caatttgttg acgagggttc aagctgccac aatttgagct atactcgtga tgtgggtgca
    12181 gtagccttta ttatagctgt tcgagttcaa ttgcttcgtg atacaggtgc agccttgtca
    12241 ccattcaatg ctttcctctt gctacaaaga cttgaaacct cttcacttcg tgtggaacgc
    12301 catgtacaaa atgctgagac aattgttgat tttcttgtca accatcctaa ggtagaaaag
    12361 gtaaattatc caaaacttgc agatagtcct tatcatgcct tggctgagaa atacttgcca
    12421 aaagatgtcg gttcaatctt taccttccat gtcaaaggtg gcgaggaaga agcacgcaag
    12481 gtcattgatc atttagaaat cttttctgac cttgcaaacg cggcagatgc taaatcgctt
    12541 gttgtccatc cagcaacaac cactcacggt caattgtcag aaaaagacct agaagcagca
    12601 ggtgtcacac caaactaaat tcgtttgtca atcggtcttg aaaatgtaga agatttgatt
    12661 gaagacttgc gcttggcctt ggaaaaaatt taaagtaaaa gaagataaac agtgggcttc
    12721 gactcactgt ttttgatttt ccctcaggca tgatataatg gttaaagaag tctagaaaga
    12781 ggaacgatat gaacgaaatc aaatgtccca actgtgggga agtctttaca gtaaatgaga
    12841 gtcagtatgc cgaactcttg tcccaagtga gaacggcaga gtttgataag gaactacacg
    12901 ataggatgaa gcaggaactg gccttggctg agcaaaaggc catgaatgag caacagacta
    12961 aactggctca gaaggatcaa gaaattgcgc aattacagag tcagatccaa aactttgata
    13021 cagaaaaaga attggccaag aaagaggttg aacagacaag ccatgaggct ctcttggcta
    13081 aggacaagga agtacagctc ttagaaaatc agttggctac cttgcgtttg gagcatgaaa
    13141 atcaactgca aaagaccctt tctgacctag aaaaagaacg ggatcaggtt aaaaaccaac
    13201 tacttttgca ggaaaaggaa aatgaattat gtttggcttc tgttaagcaa aactacgaag
    13261 cccagctcaa ggcagctagt gaacaagtcg agttttataa ggattttaag gctcaacaat
    13321 ctacaaaagc gattggggaa agcctagaac agtatgcaga gagtgagttt aacaaggttc
    13381 gtagtttcgc ctttccaaat gcttactttg agaaggataa caaggtctct tcgcgtgggt
    13441 ctaaagggga ctttatcttc cgtgagtgtg atgaaaatgg agttgaaatc atttctatca
    13501 tgtttgagat gaaaaacgaa gcagacggaa cagagaagaa gcacaagaat gcagattttt
    13561 acaaggaatt ggacaaggac cgtcgggaga agaactgtga gtatgccgtt ttggtgacca
    13621 tgcttgaggc cgataatgac tactttaaca cagggattgt tgacgtcagc cacgagtatg
    13681 aaaaaatgta tgttgttcgt cctcaattct ttatccagtt gattggtctc ttacgtaatg
    13741 cggcgctaaa ttccctaaaa tacaagcagg agttggcctt ggttcgcgag caaaatattg
    13801 acattacgca ttttgaggaa gacttggatg cctttaagct agcttttgct aagaactata
    13861 attcagcttc gactaacttt ggaaaagcta ttgatgaaat cgacaaggcc atcaaacgca
    13921 tggaagaggt taagaaattc ctgactacat ctgaaaacca actccgtcta gctaacaaca
    13981 aattggaaga tgtctctgtt aaaaaattga cccggaaaaa tccaacaatg aaagcgaagt
    14041 tcgaagcact gaagggggag tagaaagcaa aaatgaacgg tattatcaac ttaaaaaaag
    14101 aagcaggaat gacctcgcat gatgcggttt ttaaactgcg taagattttg ggaaccaaga
    14161 aaattggtca tggtggaacc ttggatccgg atgtggtggg tgttttgccg attgcggttg
    14221 gcaaggcgac acgcatggtc gagtttatgc aggacgaggg taagatctat gagggggaaa
    14281 ttactctggg ttattccacg acgactgagg atgctagtgg ggaagtgatc gcagaaaccc
    14341 ctgttttgtc tcccttggat gaaaagcttg ttgatgaagc gattgctagc ttgactgggc
    14401 ctattaccca gattccccct atgtattcgg cagttaaggt taatggtcgc aagctctatg
    14461 agtatgcgcg tgctggtcag gaagtggagc gtccagaacg tcaggtgatc atttatcaat
    14521 ttgagcgaac aagtccgatt tcttatgatg gccaacttgc ccgattcact tttcgtgtaa
    14581 aatgcagtaa agggacttac atccgtactt tgtcagttga tttgggtgaa aagcttggtt
    14641 atgcggctca tatgtcccat ttgactcgta ctagtgctgc tggcttacaa ttagaagacg
    14701 ctctttcctt ggaggaaatt gctgaaaaag tagaggctgg gcaattagat tttctccatc
    14761 ctttagagat tgggacaggt gaccttgtca aagttttcct aactccagaa gaggctacag
    14821 aagttcgctt tggtcgtttt attgagctag accaaacgga caaagaactg gctgcctttg
    14881 aagatgataa attgttagcc attctagaaa aacggggcaa tctctataag ccaaggaagg
    14941 tttttagcta gatcgtttag gaataaaaat cgggtgatag ataacaattg cttgataaaa
    15001 ccccatacta atagtagaat ggttttggga attataatat tccaattgtt gcgagttgta
    15061 ggtactcaaa taatctatat agaaatttag aggtgtgaaa tgaagcaatt taaaattctt
    15121 tcagataaat atttagagtc cattacaggt tctggtggga acttaggccc aggatttggt
    15181 gtgataattc catgatgcga aatgagtttc gagaaagggt ggagcaactt cttcaacaaa
    15241 aagaaataaa tgaaaatagt gagttgagtc acctgtttcg tcttgctata caaaatttag
    15301 acagaaatga aaaataccaa tcggtcatgg ccaatttgag tcaagggttg tcactttacc
    15361 tcatgacgca tcattaccag gcacctaagt ctgtcattga ttttggttta tggattgcca
    15421 aagctcctag ccaggaaaga gggagactgg ctttcttgca gatgcttgct caaaccctac
    15481 agggctttcg ttaaaactgt cgaacatcag agggaagact ctggtgtttt ttgctgatga
    15541 gactataaat tttgtaaaaa atgtgataga atagacgacg gataaaataa cggaggatag
    15601 catgcaaaat agaccaatca ttattggagt gacaggtggt tctggtggtg gtaaaaccag
    15661 tgtttcaaga gccattttat cgcattttcc tgatgaaaag atttccatga ttgagcatga
    15721 ttcatactac aaggatcagt ctcatttgac ctttgaagag cgtgtcaaaa ccaactatga
    15781 ccatcctttt gcctttgata cagacttgat gatcgagcag attaaggaat tgttggcagg
    15841 gcgtccggtg gacatcccga cctacgacta tacagagcat acacggagta gcaagaccta
    15901 tcgtcaggag cctcaagatg tctttatcgt tgagggcatt ttggtcttgg aggacaagcg
    15961 tctgcgcgat ttgatggata tcaagatttt tgtggatacg gatgacgatg tgcgtattat
    16021 tcgtcgtatc aagcgtgata tggaggaacg tggccgtagc cttgatagcg ttattaacca
    16081 gtacttaggt gtggtcaaac caatgtacca ccagtttatc gagtcaacta agcgttatgc
    16141 tgatatcgtc attcctgaag gggttagcaa taccgtggct atcgacctgt tgacgaccaa
    16201 gattgcaaag attttggaag aagctcgaaa cagcaaataa tcacatgtgg aggccttgcc
    16261 tcctttttct atttttccct cataatggta cataaatgga gatttttagg catatttttg
    16321 gtataataat acccataaaa gagcaagtaa atgaatagta ggtggagatg gaaaagtatt
    16381 tatcggtaac aactttgacc aaatatctga aaatgaaatt cgataaagac ccatacttgg
    16441 aacgggtcta tttaactggt caagtttcca actttcgtaa acgacctact caccaatatt
    16501 tctccctaaa ggatgaccat gcagttattc aagcgaccat ctggtctggg atttatcaga
    16561 aattagggtt tgacctggaa gaaggaatga agatcaatgt gattgggcgt gtacaggtct
    16621 atgaaccaag tggtagctac tccatcatca ttgaaaaagc tgagcctgat ggggttgggg
    16681 cgcttgcgat tcagtttgaa caacttaaga aaaaattgac agaagaaggc ctgtttcaag
    16741 aacgcttcaa gcaagctcta ccccaatttt ctaagagaat tggggtagta accagccgta
    16801 gtggagccgt tattcgagat attatcacga ccgtcagcag gcgattccca ggtgttgaca
    16861 tccttcttta tcctaccaag gttcaaggtg aaggggctgc ggaggaaatt gctagaaata
    16921 ttgcgcgtgc taatcaacgg gacgatttgg atttgctcat tattggtcgt ggaggtggtt
    16981 ctattgagga tctctgggcc tttaacgaag aaattgtggt acgagctatt tttgaatctc
    17041 gtttgccagt tatctctagt gtggggcatg agacggatgt gaccttggca gattttgtgg
    17101 cagatcgacg cgctgcaacg ccaacagcgg cggctgaact ggcaacacct gtgaccaagt
    17161 tggatgtatt aactcatttg caaaatcagg aaaaacggat ggcaacggca gtccgaaatg
    17221 ttctatctaa gaaacaagag gctttgaaaa aatgcagtca gtctgttatc tttagacaac
    17281 ctgagcgctt gtatgacggt tatttgcaac gcttggacca actgcaactg cgtttgaaac
    17341 aaagtttgcg aactcggatt tctgataaca aacaattagt tcaagcaaga actcatcaat
    17401 tagtacaatt atcacctgtt accaaaatcc aacgctatca agaccgttta ggacagttgg
    17461 acaagctctt aggtagccaa atggcgttag tttatgacgc caaggttgct gaggccaagc
    17521 gactttcgga agctttgctc atgttggata ctagccgaat cgtggcgcgt ggttatgcta
    17581 ttgtcaaaaa agaagagtcc attgtagatt cggttgagag tttgaagaaa aaagaccaag
    17641 taacgctttt gatgcgagat ggtcaagtag aattagaggt taaagatgtc aaaacaaaag
    17701 aaatttgagg aaaatctagc agaactggaa accattgtcc aaagtttgga aaatggtgaa
    17761 attgctctgg aagatgcgat tactgccttt caaaagggca tggtcttgtc aaaagagctc
    17821 caagctacgc tggacaaggc tgaaaagacc ttggtcaagg tcatgcaaga agacggaaca
    17881 gaaagtgatt ttgaatgaaa aagcaagaaa aattagctct tgtcgagtcg gctttggaag
    17941 atttttatgg agaccagcag tttgcctcta gtttacggga gtctgttctc tattctattc
    18001 atgctggtgg caagcgtatt cggccttttc tcttgttaga agttctggaa gccttgcagg
    18061 ttaccatcaa acctgctcac gcgcaggtag ctactgcctt ggagatgatt catacaggga
    18121 gcttgattca cgatgacctt cctgctatgg atgatgacga ttatcgaaga gggcggttaa
    18181 ccaatcacaa gaaattcggt gaagctatgg ccattttggc tggagatgcc ttattcttag
    18241 acccatatgc cttgattgcg caggcagatt tgccaagtca gattaaggtg gacttgattg
    18301 ccaacttatc ccttgcttca ggtagtctgg gtatggtggc agggcaagtt ttggatatgg
    18361 agggcgaaca ccagcacttg tctttggaag aacttcagat tattcatgcc aataagactg
    18421 ggaagttact agcctatccc ttccaagcgg cagctattat agctgaattg tcacctgaaa
    18481 tgcaggtgaa gctgaaaact gtgggtgaat tgattggact tgcttttcaa gtcagagatg
    18541 atgtactgga tgtgacagct agttttgagg aaatcggcaa gacacctcaa aaggatctgc
    18601 aggcagaaaa atcaacctat cctgccctgt tgggcttgga agagtccatt gccttttgta
    18661 accaaaccct ggatcaagct aatgaaaaat tagaagaaat tgcccagcag cttccctttg
    18721 aaacagaatc gattgtaagt gtagtagaaa gtttgagaat caatggctaa ggaaagagtg
    18781 gatgtactag cttataaaca ggggttgttt gaaacgagag agcaggccaa gcgaggtgta
    18841 atggctggcc tagtcgtagc agtccttaat ggagaacggt ttgacaagcc aggagagaaa
    18901 attccagatg acaccgaatt aaaactcaag ggagagaaac tcaagtatgt cagccgtggt
    18961 ggtttgaaac tggaaaaggc cttgcaggtc tttgatttgt cggtggatgg cgcgactacg
    19021 attgatatcg gggcctctac tggaggtttt accgatgtca tgctacagaa tagtgccaag
    19081 ttggtctttg cagtcgatgt tggtaccaat cagttggctt ggaaattacg ccaagaccca
    19141 cgagttgtta gcatggagca gttcaatttc cgctatgctg aaaagactga tttcgagcag
    19201 gagccgagct ttgccagtat tgatgtgagt ttcatttccc ttagtctgat tttgccagcc
    19261 ttgcatcgtg tcttggctga tcaaggtcag gtggtagcac ttgtcaaacc tcagtttgag
    19321 gcaggacgtg agcagattgg gaaaaatgga attattcgag atgctaagat tcatcagaat
    19381 gtccttgaat ctgtaacagc tatggcagta gaggcaggtt tttcagtcct tggcttggac
    19441 ttttctccca tccaaggtgg acatggaaat attgaatttt tagcgtattt gaaaaaagaa
    19501 aagtcagcaa gcaatcagat ttttgctgag attaaagaag cagtagagag ggcgcatagt
    19561 caatttaaaa atgaataaaa aagagagact tgaaaaaatt agaagattgg tgacagatta
    19621 tcaaatcggc acgcaagaag aaattgtaga acatttgaaa gaagcaggta tcactgccac
    19681 tcaggcgacg gtatcccgag atatcaaaga gttaggtatt gtcaaaattc ctttgagaga
    19741 caacacctat gtctacgaat taccaaaatc aatcgtaaaa agtctgcaac tggctgaaga
    19801 caatatcgaa tcggctgaat tgatggataa gatgatcaat ctccaagtta ttccaggaaa
    19861 tacggctttt gtaaaagctc agttaatcga tacttttgca gacaagattt ttagttgttt
    19921 gactgatgat agctcgattt tagtcattgc cagaagtgga agtctagcag aggaaatctt
    19981 tgaacaagta aaaaattggt aggtctatat gttacttgaa atttcgataa aaaactttgc
    20041 cattattgag gctatttccc tcaattttga aaaggggatg actgtcctga ctggtgaaac
    20101 gggtgcaggg aagtcgatta tcattgatgc catgaatatg atgttgggag ctcgtgcgac
    20161 gacagatgtt attcgtcatg gtgcaccaaa ggcagagatt gaggggcttt tctcagttga
    20221 gaatagtcgt cttttacagg aaatttttga tgagcaaggt ttagaattag gtgatgaaat
    20281 tatcatccgt cgggaaatct tgcaaaatgg tcgtagtatc agccgtgtaa atggtcagat
    20341 ggttaatctg tctgttttgc gagctattgg gcaacatctt gtagatattc atggtcagca
    20401 tgaccatgag gagttaatgc gtccccaact gcatatccag atgttggatg aatttggtga
    20461 tgccgctttt tgggacttga aagaaaccta tcaaacgagt tttgatgcct atcggaaaat
    20521 gcgcaagcag gttctggaag tcaagaaaaa ccaacaggaa cacaaggaac gtatcgaaat
    20581 gttggaattt caaatggcag agattgaggc agcaaacttg caggctggag aagacttggc
    20641 tctcaatcaa gagcgagata aactcctcaa ccacaaaaat attgcggata cactgaccaa
    20701 tgcctacagt atgttggaca atgaagattt ttcaagtctg gccaatgttc gttcagctat
    20761 gaatgacatg gaaagtgtcg aagagtatga tcctgaatac cgtgaaattt caagctctct
    20821 gtctgagacc tactatgttt tagaagacat tagcaaacgt ttggaagcta tcattgagga
    20881 ccttgatttt gatggcaatc gcctgatgca ggttgagaat cgtttggacc tccttcatac
    20941 tattactcgt aagtatggtg ggactgttga tgatgttttg ctttattttg ccaagattac
    21001 ggaagaatac aatctcttga caggcaataa tctttcgtct gaggacatgg aagcagagct
    21061 taagaagttg gaagtcaatc ttgtcaattt ggcaggtcaa cttgcttctg ctcgtcataa
    21121 tttggctcag caactcgaag ctgagattaa acaagaactg caagatcttt atatggaaaa
    21181 agcccagttt caggttcatt ttagtaaggg aaaattcagt cgtgggggaa atgaaatggt
    21241 tgagttttat atttcaacca accctggaga agactttaaa cccttggtta aggttgcttc
    21301 tggaggggaa ttatctcgtc tcatgttagc cattaagtct gccttttcac gtaaagaagg
    21361 caagactagc attgtctttg atgaggtgga tacgggagtt tcaggtcgtg ttgctcaagc
    21421 tattgctcag aaaattcata aaattggtca gcatggtcag gttttggcta tctcccattt
    21481 gccacaagta attgcgattg cagattatca attctttatt gagaagatta gtaatgacca
    21541 ttcaacggtt tcgactgttc gtctcttgac agtcgaagag cgagtggagg aagttgccaa
    21601 gatgttggca ggtgatgatg tgacagaagc agccctgacg caagccagag aattgttgag
    21661 aaacagggag aaataagatg acagattatt atgtaattgg agatgttcac ggaaaagctg
    21721 ggatgctgga agaccttctt aaaacctggg atggtcagac ccagttgctc tttctaggtg
    21781 atttgattga cagaggtgag gatagtcacc gtgttcttga aatggtcaag gacttggtgg
    21841 acaatcaagg ggctatctgt ttgtctggaa accacgagta tatgtttttg acttggctag
    21901 atgacccaga agaaagttat gaccattatc gtcgcaatgg tggagataca accattaact
    21961 ctatcctagg tcgtcccttg gatgcaccag ttgatggagt agaagatgcc aagcgggttg
    22021 ccgctgaagc gtcagacttg gtcgaattca ttcgtcaaat gccatttgta gtagagacag
    22081 acaagtatat atttgttcac gcaggtattg atttgacttt ggacgactgg catgaaacaa
    22141 cagattataa aaaagtatgg cttagaaaac cattccacgg agctgaaaat catactggaa
    22201 aaaccattgt cttcggtcat acaccggtct atggtttgtt acagcaagaa cgcggtacat
    22261 ctgagctttg gactacagaa gatggcaaga ttggaatgga tggaggagct gtttatggtg
    22321 gtgtccttca cgggattgtc tttacagacc aaggaatgac agaacaccac tttatcgaaa
    22381 atgacggctt tgtcgctgaa gattagtact cctagcaggg tatggtcttg tcaaaatatc
    22441 aaaaacaatt tataataaat agataccctg aaaggaagag aatcatgaac ttagaagaat
    22501 tgaaaaaacg acaggggaag atccgtaact tctctattat cgcccatatt gaccacggga
    22561 aatcaactct agcagaccgc attttggaaa aaacagagac ggtttcaagt cgtgaaatgc
    22621 aggcccagct tttggatagc atggatctag agcgggaacg tggaattact attaagttga
    22681 atgccatcga gttgaattac actgcaaaag atggggaaac ttatattttc cacttgattg
    22741 acacaccagg gcacgttgac tttacctatg aagtttcacg ttcgctagct gcctgtgagg
    22801 gtgctatttt ggtggtcgat gcggctcaag gaattgaggc tcaaactctt gccaacgttt
    22861 atctggcttt ggataatgat ttggaaatca tgcctatcat taataaaatt gacctgccgg
    22921 ctgcagatcc ggagagcgtg cgtacagaga ttgaagatgt gattggtttg gatgccagtg
    22981 aagcagtttt ggcttctgcc aaggctggta ttgggattga agaaatcctc gagcaaattg
    23041 tagaaaaagt accagcacca acgggtgatg tgacggcgcc acttaaggcc ttgattttcg
    23101 actctgttta cgatgcttac cgtggggtta tcctccaagt gcgtgtcatg gacggagtgg
    23161 tcaaacctgg tgataagatt cagctcatga gcaatagtaa gacctttgat gtggccgaag
    23221 tcggtatttt tacaccaaaa gcggttggtc gtgatttcct tgcgactggt gatgttggtt
    23281 acattgcggc gtctatcaag acggttcagg atactcgtgt gggtgatacc gttaccttgg
    23341 caaccaatcc tgcggcagaa ccattacatg gttataagca gatgaatcct atggtctttg
    23401 cgggtctcta cccaatcgaa tcaaacaagt acaatgacct gcgtgaagcg cttgaaaaat
    23461 tgcaactgaa tgatgctagt cttcagtttg aaccagaaac atctcaggca cttggatttg
    23521 gtttccgttg tggatttctt ggacttctcc atatggatgt tatccaggaa cgtttagagc
    23581 gtgagttcaa tattgatctc atcatgacag ctccgtctgt tatttacaaa gttaatttga
    23641 ccgacggtga gtctatggat gtgtctaacc catctgagtt tccagaccca actaagattg
    23701 cgaccattga agagccttat gtcaaggcgc aaatcatggt accacaggag ttcgtcggag
    23761 cagtaatgga gctagctcag cgtaagcgtg gggactttgt gactatggac tatattgatg
    23821 ataaccgtgt caatgttatc tatcaaattc ctcttgctga aatcgtcttt gacttctttg
    23881 ataaacttaa gtcttcgaca cgtggttatg caagttttga ctacgaattg tcagaatatc
    23941 gcccatctaa gctagtgaaa atggatatcc ttctcaacgg agacaaggtg gatgccctca
    24001 gctttatcgt tcataaggac tttgcttatg aacgtgggaa actcatcgtt gataagctca
    24061 agaaaatcat ccctcgccaa caatttgagg ttccaatcca agcggctatt ggacacaaga
    24121 ttgtcgctcg tactgatatc aaggcccttc gtaagaacgt acttgctaaa tgttatggtg
    24181 gtgacgtttc tcgtaagcgt aaactccttg aaaaacaaaa agctggtaag aaacgcatga
    24241 aatccatcgg atcagttgaa gttccgcaag aagccttcct cagcgtcttg agtatggatg
    24301 aagaataagt tatcagaaat gcctttcctt gttcacagga aatttatata agaatcaata
    24361 gtaaagcagg cttagatggt ctgctttttt agtttttata ctcttcgaaa atctcttcaa
    24421 accacgtcag cgtcgcctta ccgtatgtat ggttactgac ttcgtcagtt tcatctacaa
//
LOCUS       DBERYS010000028        24006 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.028, whole genome shotgun sequence.
ACCESSION   DBERYS010000028 DBERYS010000000
VERSION     DBERYS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.028"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(11..895)
                     /locus_tag="ACL8LA_001299"
     CDS             complement(11..895)
                     /locus_tag="ACL8LA_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1813054.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(899..1285)
                     /locus_tag="ACL8LA_001300"
     CDS             complement(899..1285)
                     /locus_tag="ACL8LA_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGS1813055.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1278..2621)
                     /gene="cysS"
                     /locus_tag="ACL8LA_001301"
     CDS             complement(1278..2621)
                     /gene="cysS"
                     /locus_tag="ACL8LA_001301"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGS1813056.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2708..3592)
                     /locus_tag="ACL8LA_001302"
     CDS             complement(2708..3592)
                     /locus_tag="ACL8LA_001302"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813057.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3604..4221)
                     /gene="cysE"
                     /locus_tag="ACL8LA_001303"
     CDS             complement(3604..4221)
                     /gene="cysE"
                     /locus_tag="ACL8LA_001303"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGS1813058.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4237..6450)
                     /gene="pnp"
                     /locus_tag="ACL8LA_001304"
     CDS             complement(4237..6450)
                     /gene="pnp"
                     /locus_tag="ACL8LA_001304"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGS1813059.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            complement(7135..8001)
                     /gene="metF"
                     /locus_tag="ACL8LA_001305"
     CDS             complement(7135..8001)
                     /gene="metF"
                     /locus_tag="ACL8LA_001305"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGS1813060.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8065..10314)
                     /gene="metE"
                     /locus_tag="ACL8LA_001306"
     CDS             complement(8065..10314)
                     /gene="metE"
                     /locus_tag="ACL8LA_001306"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGS1813061.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10575..10841)
                     /locus_tag="ACL8LA_001307"
     CDS             complement(10575..10841)
                     /locus_tag="ACL8LA_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1813062.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10967..11413)
                     /locus_tag="ACL8LA_001308"
                     /pseudo
     CDS             complement(10967..11413)
                     /locus_tag="ACL8LA_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11569..12570
                     /locus_tag="ACL8LA_001309"
     CDS             11569..12570
                     /locus_tag="ACL8LA_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS1813063.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(12638..15040)
                     /gene="pheT"
                     /locus_tag="ACL8LA_001310"
     CDS             complement(12638..15040)
                     /gene="pheT"
                     /locus_tag="ACL8LA_001310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGS1813064.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(15117..15626)
                     /locus_tag="ACL8LA_001311"
     CDS             complement(15117..15626)
                     /locus_tag="ACL8LA_001311"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813065.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(15626..16672)
                     /gene="pheS"
                     /locus_tag="ACL8LA_001312"
     CDS             complement(15626..16672)
                     /gene="pheS"
                     /locus_tag="ACL8LA_001312"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGS1813066.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(17203..17478)
                     /locus_tag="ACL8LA_001313"
     CDS             complement(17203..17478)
                     /locus_tag="ACL8LA_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGS1813067.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(17585..18424)
                     /locus_tag="ACL8LA_001314"
     CDS             complement(17585..18424)
                     /locus_tag="ACL8LA_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1813068.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            18566..19414
                     /locus_tag="ACL8LA_001315"
     CDS             18566..19414
                     /locus_tag="ACL8LA_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS1813069.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(19507..20424)
                     /locus_tag="ACL8LA_001316"
     CDS             complement(19507..20424)
                     /locus_tag="ACL8LA_001316"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGS1813070.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            20903..21051
                     /locus_tag="ACL8LA_001317"
                     /pseudo
     CDS             20903..21051
                     /locus_tag="ACL8LA_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(21377..21670)
                     /gene="rpmA"
                     /locus_tag="ACL8LA_001318"
     CDS             complement(21377..21670)
                     /gene="rpmA"
                     /locus_tag="ACL8LA_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGS1813071.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(21687..22031)
                     /locus_tag="ACL8LA_001319"
     CDS             complement(21687..22031)
                     /locus_tag="ACL8LA_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGS1813072.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(22047..22361)
                     /gene="rplU"
                     /locus_tag="ACL8LA_001320"
     CDS             complement(22047..22361)
                     /gene="rplU"
                     /locus_tag="ACL8LA_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGS1813073.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(22397..22484)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(22543..23247)
                     /locus_tag="ACL8LA_001321"
     CDS             complement(22543..23247)
                     /locus_tag="ACL8LA_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGS1813074.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            23649..23948
                     /locus_tag="ACL8LA_001322"
     CDS             23649..23948
                     /locus_tag="ACL8LA_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGS1813075.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         6950 a         5307 c         4484 g         7265 t
ORIGIN      
        1 attaacttct ttacaaacca actatagttg acaaagaacc taaaatcaat tgataacaca
       61 aggtcaggtc ggtcaactct ttcaactgaa gccctgtcaa ctcttcccat ttatcaatct
      121 tgtattggag agaattgcgg tgcagataga gttgctgggc tgttttagtg agaacagcac
      181 tattttccca aagagagaga atgatttcct gaatctgatc ttgatccaaa atcatctggt
      241 gtagacattc tttaattggc ttcaagtcca cgagtctttc tcccatactc caaagataga
      301 gctgagaaaa agtatgaaca ccttggtgac cctgacgcca ccatgtcttg aacaaatccc
      361 gctcagcttt gattaagtct gatagggctt gatgtcctgt ctgagaccaa acctgaccca
      421 acatgataga aagacgaagt ccaaagtcat actcaaccgc ttcaatcgta tcactcaaaa
      481 tatctcttac agaagtgtat ttgtcttgtt gaagcacgaa aacataatcc tgagctccga
      541 cttgtagcac tgtctgacaa ttcggaaaaa gagtccgcat catatctagc caagaagtca
      601 gattttcctg ctgaaaataa gaaagatggc aataaaccaa ctgaatcttt ttaaaaactt
      661 gcggtgcctg tcccttgccc tcaaccagat aggaatacca agggtttagc gaacgagcct
      721 gctcctgctg ggtcaaaagg gcaaccaact gcttttcacg ctcgctgagc ccagcttcct
      781 ccagcaaaat ccactgctga gaagctaaag gaagcgtgag atagccctct ttctctactg
      841 gttggtctga aatccgagcc tcaggaaacc agtcttgtag ttcttttgcc ctcatgttct
      901 agccctccac tttttggatg caccatgaaa ccaaactctc aagacgttcc agattctcag
      961 tcatatggag atagcccata actgcttcaa atcccgtgga catacgataa gtcacgacat
     1021 ctgcattttt agcctttgtg tgactattgg tattgcggcc acgtttgtag atttcttctt
     1081 ctttttccgt taggatctgc tcctccaaca tgagagcaat cagacgagcc tgagccttgg
     1141 ctgacacgta cttggtcgcc tcttggtgga gtttattggg cttggtcata cctttgagga
     1201 tgaggtggcg gcgaatatac atagaataca ccgcatctcc ctcaaaggct agcgcaatcc
     1261 cgttaatgag attgacatca atcacgtgtc cacctcactc catccttggt atcaaggagc
     1321 ttaattcctt gagtaaccaa ttggtcacgg atttggtctg ctgtctcaaa gtcacgattg
     1381 gcacgcgcct cttggcgttt ttgaatcaag tcttcaatct ctgcatccaa aacttcctca
     1441 acaaagacaa ttccaaaaat ttctaacata tctgcaagag cttgcttgac acttgcatca
     1501 tagttccctg agttgatcca tttggccatt tcaaagacaa ctgtgatacc gttggcagca
     1561 ttaaaatctt catccatagc tgctacaaac ttatctttaa agttttgtaa ctcttgggca
     1621 tccacgtttc ctgtaaatgg ttgttcgtaa gtattcttca gatacttgag attggtctcg
     1681 gcatcgcgaa ctgccttttc cgtgaagttg ataggcttac ggtagtgctg ggtcgcaaag
     1741 aagaaacgaa gtacttgccc atcaagagtt ttaagggcat cgtgtaccgt aatgaagtta
     1801 cccaaggact tagacatttt gacattgtca atattgacaa agccattgtg catccagtag
     1861 ttagcaaaag ccttgcctgt tttagcttca gattgggcaa tttcattggt gtggtgtgga
     1921 aactctaggt cagctccacc accgtggata tcaatggtat cacctaaaat ctctgtcgac
     1981 atgactgaac actcaatatg ccaacccgga cgtccaggtc cccaaggact atcccaagaa
     2041 atctcacctg gtttggaaga tttccataga gcaaagtcta caggattttc cttacgagcc
     2101 gtttcttcat cggtacgacc tgaagcacct agctccaaat cttccaaggt tttattagcc
     2161 aatttagcat agttgtggga tttttctaca cggaaataga catccccttg actctcatag
     2221 gcaaagcctt tctcgatcaa gtcttccaca aaacggatga tgtctgccat aaactccact
     2281 acacgcggat ggcgagtcgc aggtttcacg cctaaggccg tcacatcctc acgaaaggca
     2341 gcgatgtact tatccgcaac ctcctgaggt gtgatgcctt cttccctggc acggttgata
     2401 atcttatcat ccacatctgt aaaattggaa atataggcaa ccttataccc acggtactca
     2461 aaatagcgac gaatcgtatc aaaagctacc gtcgaacggg cgtttcccac gtgaatatag
     2521 ttgtacacgg ttggcccaca aacatacatc ttgatcttgc cgtcctcaat cgggacaaat
     2581 tctcgcaaat cacgagacat ggtgtcatag attttaatca taaatcataa tcaggaaagc
     2641 taaaatccaa gaactgttag tttcatcact aaactaagag ctcaagtaaa tttcagtccg
     2701 aatgtctcta cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgtgtct
     2761 gtttgacaaa gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg
     2821 caatctgaaa ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt
     2881 agcttccata gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg
     2941 tattagtcga taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat
     3001 ataagcgact ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg
     3061 attcatgttc ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac
     3121 tatctgctaa caaaacttca agatgggaaa catttgctaa cggataaggt cttctatcct
     3181 tacctaacca agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag
     3241 gatgattctc taaaaaaata cgttctgtct gaaaagtgac tgaccgaatg gggaaagaag
     3301 ctgtttctct ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg
     3361 gatgaaccaa tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa
     3421 caagtttgcc tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca
     3481 tgttagagag atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt
     3541 ttttcgcctc agatagctcc tcttggctta acttgtttct tgcttgaatc atataggtat
     3601 cctctacaaa ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata
     3661 atactcccgt ttttcttcga cttcgtgaat aacaggctca tctttcttac catgaagacg
     3721 gacaatcttg gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac
     3781 tgctgcagca ccgaccttgg cattttcacc aatttccaca ggcccgataa cttgggcatg
     3841 ggctgatatg agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt
     3901 tcccccgaga gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc
     3961 aatcaccaga ccagaaccat ggtcaataaa aacacctgaa tcaatctggg ctcctggatg
     4021 aatctcaatc tgagtccaaa agcgccaaaa ctgactgtac atacgagcta atagtttgaa
     4081 gccgtgcttc cagagaaaat gcgagagacg gtgggccgcc aaggccttga cacctggata
     4141 agtcagcaaa acctccaaag tggtgcgggc cgctggatca ttttctttta caatatcaat
     4201 ggtttcgcgc caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc
     4261 tgtaaattct ttcttaggtt tgtgatcctt ttgatgacgt gggcggtgag ggcgctcaga
     4321 cttttcacct ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga
     4381 ggcatcgata cggccttttt catcaatttt gataacctta acatcaactt catccccgat
     4441 ttctaccaaa tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag
     4501 ggcatctgtc ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt
     4561 agcacggtaa acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc
     4621 acggttaata gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc
     4681 aatcttaaca cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac
     4741 aatcttaatc ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa
     4801 ttctggacga acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt
     4861 cttggcttga gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc
     4921 catttgaagg gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa
     4981 gtgatcttcc aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag
     5041 tcccatagca ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag
     5101 agttcccgca cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac
     5161 tagacggata gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc
     5221 accaagggca ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc
     5281 tacagaatat tgagggaagt tatagtggtg cataaagcgt ttcttgtact ccagatccaa
     5341 accatcaatg atttgagttt ctcccatcgg agccaaggtc aagacagaaa gggcttgcgt
     5401 ttgtccacga gtgaagagac ctgaaccgtg cacacgagga aggaagtcga caaccgcatc
     5461 caaaggacgg atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa
     5521 acgtcgcact tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg
     5581 gtcaaattct tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac
     5641 tacttgagtc gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc
     5701 actgttgtag gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac
     5761 ttctgctttt tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt
     5821 gacagcttcg tgccctttaa ggagcgcttc caacatgatt tcttctgaca attctttagc
     5881 accagactct accatgttga tagcgtgctt ggttccagct actgtcaatt caagaagaga
     5941 ttgctctgct tgttcttgac ttgggttgat gatgatttgg ccatctacat atcccacttg
     6001 taccccagca attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc
     6061 aaacatagca gccattggtg cagacgcatt ttcatcataa gaaagcactg tattgatgac
     6121 ttggacttca ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg
     6181 cgctgtcaag gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa
     6241 cttcccagcc gcatacattt tttcttcgta gttgacttgg agtgggaaga aatccccagt
     6301 tgccattttc ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac
     6361 aacagagcca tttgcttgct tagcaacctg accagtttct acaattaact cacgacccgc
     6421 aaaagtcgtt tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata
     6481 cgccttgcct acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac
     6541 tgacgaagtc ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg
     6601 ttcaattaca caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag
     6661 gaaggtgacg tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga
     6721 ccaaaattca agtcatcaag ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc
     6781 gaacgacgga gcgactgctc ctagggagat ttatcttttt ccacagagtt gtaggcaagt
     6841 tcagttttca agatacatca ttagaaaggt ttaatactaa agtatctaaa gctttcacgc
     6901 taatcgctat cgggcgatta gctaaatgct ttactaactc tctcgtcaaa taacatcgat
     6961 ttgactcact cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc
     7021 atctttgtat caagtacgta cagaatttat tttatcatat ttttcttaaa aagtgaggtc
     7081 tttaccatta aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc
     7141 tagagactgg tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc
     7201 attattcatc gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg
     7261 gtccactgca taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc
     7321 taagatggct ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag
     7381 agcctgattt cgattcagaa ttggcataat tcctgcatga atgggaacat caatcccagc
     7441 caaaatacac ttgtcctgaa aatcatagaa gcgctcatta tcaaagaaga gctgagttac
     7501 gaggctcgaa cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt
     7561 tggcgaatct ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg
     7621 ttccttgata aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc
     7681 tggaataata tccccacgaa gagccaagat tttctgcacc ccaactttgt ccaagtcagc
     7741 aatagtttca gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg
     7801 aatcgccaag tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa
     7861 tttattattg ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa
     7921 ggctgaaata atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag
     7981 tgacggtgtt tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac
     8041 cactgtatgg agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac
     8101 aaggcggatc aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg
     8161 gttgatccaa actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat
     8221 ttcgccttca tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt
     8281 ttggaagttt ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tagcttcaaa
     8341 ggaaataacg tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta
     8401 acacatgtga gtgtggattt gtgtgtctgg cgctactgtt gagtgtacca agcggaaggc
     8461 aggaatagcc cagtcaaggt agtcttcgta ccagtcgcta cggcggagtg gcaatttttc
     8521 acgaagagca gcctcgtcga tttggatgat tttcacacca gcagcttcaa ggtcaagtac
     8581 ttcatccttg atagcaaggg cgatttgaag agttgaatcc ttgatagaga tgtcttcacg
     8641 tgggaatgac cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt
     8701 tgtacgactt tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc
     8761 accccagatg attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt
     8821 agagaagagg tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa
     8881 ttcaccgtga acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat
     8941 cgtttcagca aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa
     9001 acgtttggca cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc
     9061 tggaagtttg aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt
     9121 gtagtctgcg tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg
     9181 ctcagtcgca aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat
     9241 agcatccaag tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag
     9301 tgctggttca aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct
     9361 tgtcaaaacg atgttttcag ctgggatttg ctcaagaaca gccaagctct tttcgtagtt
     9421 gttgcgccag atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa
     9481 gccaccttta acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc
     9541 tactggcaag tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag
     9601 caagacttca agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc
     9661 tttttcttca gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc
     9721 accaagttca gccaatttag caaaaacttc ttggtaagca accactaagc tatctacgaa
     9781 gtcgtctgct ttcacgcctt cttcaaagtc tgacaattga aggaaagtga atggacctac
     9841 aagaacagga cgagtgttca atccaagttc ttttgcttct tggaactcat cgaaaatctt
     9901 gtgaccagcc aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt
     9961 agtgttgaac catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc
    10021 acgtcccaaa gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg
    10081 caccacgttg aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga
    10141 tggaatttca gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt
    10201 tgctgctgct aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa
    10261 ttttaattca cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg
    10321 tcctccaaaa tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta
    10381 aaaaattagg gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac
    10441 ttgaagcaaa cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa
    10501 tactaaaaca tgttattagt aattcttata agtgactatg acctgttatt agaaaagact
    10561 ataactgatt ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct
    10621 agactggtta gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtctccatc
    10681 ctagctatga caggctggct tattccactg acttcttcca gctttttctg actgattcct
    10741 tgttcatacc tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg
    10801 atttcctcct tgctaaagag ctcagatgga catccttcca attactccca atagcactat
    10861 tcttcatcac ttaaccctct tttttttacg tctatgtatt tttaaaaaaa tgagcgaatt
    10921 atgattcgat agattgacca gcgggtttaa agttggtgct agcctatttc ttaagcgctt
    10981 ttccttttct aggataaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc
    11041 aatagtaaat ggttgaaatt cccacgttaa cccctttagc catcaccatc atttcaggcg
    11101 aaaatttttg gttatgatag tggagaatct tttcctttag tttcttagtc aagcttgatt
    11161 tcttgaccga gtgcttgcga ttattttcat aagactgttg agcgtagtcg gcagaataaa
    11221 tctctttgaa gcgccctttt ccaagacatt gtcggactgt cccatacttg atttcagtat
    11281 ggatagtttg aggagctttt ccaagtagag aggcaatttc tctatttgat tttccttctt
    11341 ttttccatcg ttcgattaag cgacggctat cgattgtcaa atgtttgcct tttgtagtat
    11401 aattgtcttg catttctgtg ccttttaatc atttcaatct taaattggac tttttttact
    11461 tgggttgtac ttaatctatg aggaagacaa gaaaaagaat atcaatcaag taaagtcaca
    11521 aagtcacatt agctccgagc aaccattgca aattgaggta ctcacacaat gattaaaaca
    11581 tttctctctg ccctttcggt cattctcttt tctatcccta ttataactta ttcttttttc
    11641 ccatcttcta atcttaacat ttggctatct acccaaccta tcttggcaca gatttatgcc
    11701 ttccccttag ctactgcaac tatggctgct attttaagtt tcgtattttt ttccctatct
    11761 ttttacaaga aaaataaaca aatacggttt tactctggca ttttgctctt actatcgctc
    11821 atattactat tattcggaac agataaaacc ctttcttctg catcaaataa gactaaaacc
    11881 ttaaaattag taacttggaa cgtcgctaat caaatagaag cacaacatat tgagcgaatt
    11941 tttagccatt ttgacgccga tatggctata ttccctgaac tagctaccaa tatcagaggt
    12001 gagcaagaaa accagagaat caaactattg tttcatcaag ttggactttc tatggccaac
    12061 tatgatattt tcacttctcc acctaccaat agtggaatag ctcctgtgac tgtgattgtc
    12121 aagaaaagtt atggtttcta tacagaagct aaaacttttc atacaacacg gttcgggaca
    12181 attgtattac attcgagaaa acaaaatata ccagatatca ttgccttgca tactgctcct
    12241 cctctgccag gtttaatgga aatctggaag caagacttaa acatcattca taatcaattg
    12301 gcttcaaaat atccaaaggc tattattgca ggtgatttta atgcaactat gcgtcatgga
    12361 gcacttgcaa aaataagctc tcatagggac gcattaaatg tactgccacc ttttgaaaga
    12421 ggaacttgga atagccaaag tccaaaactt tttaatgcaa caatagatca tattttattg
    12481 cctaaaaacc actactatgt taaagattta gacattgtaa gttttcaaaa ctctgatcat
    12541 agatgtattt ttacagaaat cacattttaa ttattttata taaaatcacc cctctagtgt
    12601 tcataaacta gagggggaat ttgtatcata ctatcgttta acgcacttct gcattgactt
    12661 tttcttcgag cgatgcttgg attttttcca tatagcgtgc gacttcttcg tccgttaagc
    12721 tatcttctgg attttggaag gtcaagctat aagccattga cttcatacca agtcccaatt
    12781 tctcacctga gaagacgtca aagagtttga tatctgtcaa acgtttcacg ccggcagctt
    12841 ggatagcatc tacaacttct tgatgagtca cttctgcctt gaggagaagg gcaacgtcac
    12901 ggctgactgc cgggaatttg gtgatttcta caaatggagt cgctggctga agcgcagctt
    12961 cgatagctga aaggttaagc tcagccacat acgtttctgg aatatcgtaa gccttggcag
    13021 tgactggatg cacttggcca aggaaaccaa gaacttggtc accgaatgaa atcacggctg
    13081 tacgacctgg atgaaggcta gcgatttcag atgttgctgt ataggttact tggagtccca
    13141 aacgagtaaa tagggcttca aggattccct tagcatagaa gaaatcaact ggaactgctg
    13201 ctgtttggaa atctttttca gcaaccaagc ctgtcaaggc aaaggcaaaa ctgttgattt
    13261 catttggaag ttcttctttt ggattacctg tttgttcaaa gacttttcca atctcgtaaa
    13321 gggccaagtt tttattctta cgagccacgt tgtaggcaac ggtatcaagg atacctgaaa
    13381 tcatattttg acggaggact gaacgatcca cagtcattgg ccacatgagt tccgtaaggt
    13441 tacttggttg agccgtaaac tcaactgctt tttcaggagt tgttagagta taggtgatga
    13501 tttctgtcaa acctgctcct tcagcaatag tacgaacttg acggcggagt ttttgtgtgg
    13561 ctgtcaattc acctgctgta ccatcgtctt ttggtagact agttggcaag cggtcataac
    13621 catagatacg agcaatttct tcaaagaggt cagcttcgat tgtgatatcc caacgacgac
    13681 gtgggactct gactgtaaag ctgtctgcat ttccagaaag accaaagcca agacgacgga
    13741 agacgtcttc tacatcagca taagacagct cagttccgag gacacgatta acatcagcaa
    13801 gggttgaaga aacttctaca tctgaagtat caagctcacc cgctgaaacg atgcccttac
    13861 gcaccgtcgc acctgcaagt tccgcaatca ggctagctgc cgcatcaagg gcttcattaa
    13921 ctgttgccac attaattcct ttttcaaagc gagaagatga ctcagaacga aggttcaggc
    13981 gaccacttgt cttacggata gatttgccat tgaaaacagc agcttcaagg acaacacgac
    14041 tagatttttc agagatttct gttgcttgac cacccatgac acctgcaagg gctactggct
    14101 tgtctgcgac agtgatgact aggtcattca cgtccaagtc acgttcttca ccgtccaagg
    14161 tcaccaattt ttcaccagca cgcgcttcac gcacacggat gtcagtccct tcaaagttat
    14221 ccaagtcaaa ggcatgcatt ggttgaccaa aatagagcag gatgtagttg gtcacgtcca
    14281 ctacgttatt gatgggacgg attccttcgt tcatgagaag attttgcaac cattgtggac
    14341 ttggtgcgat ggtcacattg tccaagatac gagctgcata gtaaggcgcc ttgtctgtct
    14401 caatgctgac agaaagggca tctgccgcag cttcattagt ttctgttaga gtaaattctt
    14461 taaagttgac tgccttgtca tagatggctg ccacttcgtg agccactcca cacatagaaa
    14521 gggcatctgc acggtttggt gtgatggaaa gttcgatgat ttcatcatcc aagtctaggt
    14581 aagaaaagac ttcctcacct ggcacggcat cttcaggcaa gatttggatg ccatctgcga
    14641 attccttagg cacaactgag tcagaaattc ccaattcacc aagtgaacag atcattccaa
    14701 gtgactccaa accacggatt tttccttttt tgattttgta gttatcagcg atacgagctc
    14761 ctggaagagc caccatgacc ttgatcccag cacgcacatt tggggcaccg caaacgatct
    14821 gacgctcttc ttcgccaacg ttaacctgac aaacatggag gtgagtctct ggcacatctt
    14881 cgcaagacaa gacctcaccg acgacaattt ttgagagacc agcagctggt gattcgacac
    14941 cctctacctc gattcctgta gttgacattt tttcagccaa ctcttgtgat ggcacatcaa
    15001 tgtccaccaa ttcttttaac catttataag atataagcat aatttagttc tccagaatga
    15061 ccattgccac actggttctt ttcctttcct atcatttcaa tagaagaatc ctcttcttac
    15121 cttaatttct ttctcagtaa ccaatccgta tctacttttt gaccaaccat aaaatgatgt
    15181 tggctaaatt tttcaaaacc atatcgatta taaaacgctt gagcttttgt attatgctcc
    15241 caaacaccta gccaagccca agaaaaacta ttttttgtag caagttcaag tgcgaattca
    15301 aacagttgct tacctagtcc aaacccttgg aatttttgta gcacatagag gcgttgaatt
    15361 tcaaaagcgt cctctaattc tctctcagtt tgagcacttc cccagttgac tttgagaaaa
    15421 ccagctatct cctcctcatg cataatgaaa taggtttcag agtcaggatt tcccaactca
    15481 gttgacaaag ttttcagact ataagcctct tcaaagtatt cctgtaactg ctcttccgta
    15541 ttatcatacg caaaggtttc acgaaaggct tgtttggcaa ttttagccaa cacctcaaca
    15601 tctgccattt ctacttttct aatcattatt taaactgttc tgagaagcgg acatctcctt
    15661 ggtagaatcc acggatatcg ttgattccat aacggagcat agctacacgc tcttgtccaa
    15721 gaccaaaggc aaaaccagag tatacagtcg catcgatacc actcatttca aggacacgtg
    15781 ggtgaaccat accggctccc ataatttcga tccaacctgt tttcttacat acgttacagc
    15841 cttctccacc acacttgaag caagaaacat ccacctcaac agatggctct gtgaatggga
    15901 agtaagatgg acgcaaacga atttgacgct cttcaccaaa cattttttgg acaatcaact
    15961 gaagcgttcc ttgaagatca gccatagaga tatttttccc aactaccaag ccttcgattt
    16021 ggtggaattg gtgactgtgg gtcgcatcgt ccgtatcgcg acggaagaca cgccctggcg
    16081 agatcatctt caaaggacct ttagaaaaat catgggcatc catagcacgc gcctgaactg
    16141 gagacgtgtg ggtacggagc aagatttctt cagtgatata gaaagtatcc tgcatatcac
    16201 gagctgggtg gtcttttgga aggttcatac gttcaaagtt atagtagtct tgctccactt
    16261 caaaaccatc cacgacttga taacccatac cgatgaagat atcttcgatt tcttcactgg
    16321 tttgtgtcaa aacgtgacgg tgaccagtcg caactggacg acctggaagc gtcacatcga
    16381 tactctcgct agccagttga gccgcgactt tcttttcttc caagagctta gctgtttctt
    16441 caaaagcagc tgtcaagaca tcacgagctt cattgacgtg tttcccgatg attggacgca
    16501 tctcagcaga aacatctttc atccctttga ggatttcagt gagcgaaccc tttttaccaa
    16561 ggacagagac acgcaaatct tgcatctctt tttcatttcc agcagtaatc tgcttcaagc
    16621 tagtcagcgt ttcttcgcga agcgctttta attgttcttc aatagttgac atatttcctc
    16681 catcagtctc tcgtagataa aaagaaaacc acatgccaaa aactccactc ggagcgttga
    16741 cacgcggtac catccgtttt catctgacaa gtcagacctt catttctaaa tccatgcgca
    16801 agtgaattca cccagctttc atatagagag cttgcagtca cggctctcct ccctgatata
    16861 cttcccttgg gctactagtc tttcagattt ctattcaatt accacttagt ttatcagatt
    16921 tttaccattc ttgcaagacc tatcttactt ctgcttgtta gcttattctt atctaacttt
    16981 atagcggatt gaaataagat gaaaacaaat cgattgggaa agtaaaatta atttctataa
    17041 atgttttagc aattgtttcg tactatttta gattcagtct actatatata caatattttc
    17101 ggaacattca actttttaac tctatttatt actagatttc ataattaaaa agcctactga
    17161 ccaagctaga aagcttgata caataggctt tttaaagact gattatttaa cagcgtcttt
    17221 aagagcttta ccagctttga atgctggtac tttagaagct gcaattgtca tttctttacc
    17281 agtttgtggg ttgcgacctt tacgttctgc gcgctcacga acttcaaagt taccaaaacc
    17341 gatcaactga actttttcac cagctgcaag atagtcagct actgctgcaa atacagcttc
    17401 aactgctgct gctgagtctt tcttagtcaa ttctgtagct tctgctactt tagcgatcaa
    17461 atcttgtttg tttgccatgt taatgattcc tccaaataat ttctaattaa caaatataat
    17521 catatcctaa aatccctttt aggtcaagtt aaaaacgcta tttcttccca ttttatttat
    17581 ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg tgagtttgaa
    17641 taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt aagctttctt
    17701 tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct gctacagcac
    17761 gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta gttccacgac
    17821 ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacaacacgg atattgagaa
    17881 gagagctcaa caagccagtt acacggctaa ttcgtccacc tttgacaaga ttttccaaag
    17941 ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga gataaaattg
    18001 cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg aatttcaggg
    18061 cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg ctagcacctt
    18121 ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca agaatctggc
    18181 caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt ggctgacttg
    18241 ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct tctttcaaat
    18301 ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt gtaatatcta
    18361 attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc ttaatttttg
    18421 tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc taattatatc
    18481 aaaaaaaaga ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat ttgatataat
    18541 caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccccattt ttaccatttt
    18601 actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc atcatctctt
    18661 tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa tccctgtttc
    18721 cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga tctttggagc
    18781 caaatccctc tattctagtt tactaggaac cttagctttg tccgcctggt tagctttttt
    18841 tgagcatatt ccccttcata ttgatcttca aggtgattta ctaatcacag cccttatagc
    18901 gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta caactggcgg
    18961 aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag gaaaactgct
    19021 ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg atttgagatt
    19081 ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt tgattggtga
    19141 aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc aacttgctaa
    19201 ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag gctactatag
    19261 taaagaaaat ttgaaaatca tctactgtat tgtcggaaga aatgaaattg tgaaaacgaa
    19321 ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag cccatgaaat
    19381 cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtaatgtcgt gacctcaaaa
    19441 gttagactaa atcatctatc ttttgggtta cagacaacct cttttttgtt ttatttactc
    19501 aagctcttaa gaccaattcc gagttacttc ttcatcagcc tttaactgat ccactaattg
    19561 gtcaactgag tcaaatttgg tcatatcacg aatgcgatca agccaataaa ccatgacggt
    19621 ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg cttcttctcc
    19681 atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc tttgaatctc
    19741 aacatcaacg acataaacgc catctgctgg catataagta cggtctaaaa gcactaaatt
    19801 cgctgtcgga taaccaattg tacgaccacg agcattacca tgaaccacca tacctcttga
    19861 tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta aaatagcttg
    19921 acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa caatgataac
    19981 ttctccatca aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac caaatgtata
    20041 atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg caaagaattc
    20101 ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt cttctacacc
    20161 ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa acaaatctgg
    20221 atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga cgataggcaa
    20281 taaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc ccttatgtat
    20341 gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat ctttttggtt
    20401 ttttatagga atagtaataa tcataaaata attatatcat agcgatagct atttctggaa
    20461 cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa aaagcacttt
    20521 attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt gtaggacatc
    20581 ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt catactatgt
    20641 tatgtatgaa aaagcaatgc aactaactcc tgagaacttt aaattactaa ttggtgccaa
    20701 aaaggtagaa tttagaatcg aggtacacct atggctgtaa aatttacaaa atgagacaac
    20761 ttgggcaaga tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt cactttccct
    20821 gatgacaaag aaaaaagcca aaacagcaaa gaaaaactag atgactgctt tccaacaact
    20881 cccatctagt gtgcttcaga ctgggctatt tttctctcca tctgttagct tggattctca
    20941 gaccgtttca gctaaagaat atcttttccc ttatcagaag gaacggctca aaccattcag
    21001 acaagtgaag ggacgacaag ccaatatttg aaaccagata gcagttctta tagtcaattg
    21061 aaataaaatc tgaagaaatc gagtaggaaa ctcatatcaa tttttaacag tgttctattc
    21121 cagattcata ctcaatgaaa attaaagtgc aaactaggaa gttagccgca ggctgtactt
    21181 gagtacggca aggtgaaatt gacgtggttt gaatttgatt ttcgaagagt atcaatccac
    21241 tatacatatt aaaaagtatg ggtatcaaaa taaaaaaaca ggctctccga aaactcggaa
    21301 agccttattt taagctgttt ctagcttcct cacctttaca tttcaaggtt cgggataaaa
    21361 ggttcaatga acctttttat tttgcgattg ggtaaacaga aacttgtttt ttatcgcgtc
    21421 ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatcg tctccaccac
    21481 gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg attgatccac
    21541 ctgttacagt ttgtccgtca gctgctttag ctccaagacg ttttgcttgt gaatcacgtc
    21601 cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg ttaagagtca
    21661 tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt ttggacgaac
    21721 tcagaatagt tctccgataa gtttgccata cctaagaaaa atgattcaaa gaataactgg
    21781 gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag atagccacct
    21841 tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat agaattgata
    21901 aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta ttcgccactc
    21961 tcggcgtgtc cagtaatttc cgcactcctc agctcgccat cttcggctct ctcaaagact
    22021 gcctgtatca tgtgttctcc ttaaaattaa gcgttgattg cgttgatgac aacttttgta
    22081 tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta cttgtaagta
    22141 accactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc tccagcaaca
    22201 agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt aaaagtaact
    22261 tcttgaccag cttcaacgtt caatttttca acgtaaactg cttgaccaac ttcaacttta
    22321 acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct cctatgattt
    22381 ttatgggttt ccccgatttt ttgtgaagac tcgcctagca tcgtgggacg aaccacttaa
    22441 acttgaataa tcaagcaacg atgttcgtgc ggttgcacag gatcgtgcat agtcaactct
    22501 tcaagtatag catatctcct attttcttac aagtaataac acctaaaatg aagctttttc
    22561 ttttactttt ttctgccaag aggcaaaaag catgctgagg taaaaaacgc tcatcataat
    22621 aggaacacca agaatggtct tttcatgata gaaaatcgtc aaataggctg aaaagacaac
    22681 gccaaggaca aaactactaa gcaggctaac aaatatgaat ccttcacgca aaaaaggagt
    22741 gtgcttggtt cggaaataat ctccaaaagc cagcatggtc cgtttgatat tccctgtcat
    22801 aaaagcgtta ttataggcaa tacccgacac ttctccaaaa gcagttgtca ccagtcccat
    22861 acagaaggcc aagggcggca ctagatagat attatccaca gtttgcggca caaaagcaat
    22921 aatgattgat aagattgcca agggaatcaa ggacagaata ggttttttca caattctcaa
    22981 tttttcctta taaatcgtta ataaaaagac tcccatcata aacgctagca aggtgagaac
    23041 cttgtcccta acatccgaaa cattattttt aattaattct actgaaagaa agacaacatt
    23101 tccagtttgt ccagctacaa gggtattccc gcgaacaata aaagtgtaag catccacata
    23161 tccagcacaa aacgtcaaaa aaagtgctaa ccttttagac tgacgtgata tttttcttat
    23221 aggtaataac ctcattttac ctcccattgt attttctctt agaaatattg taccattttc
    23281 tttctaaaaa atcgtaggct accatttaga ttttactatt agcataaaaa taataataga
    23341 caactattta tccaaaaata gatagatgta acatgtttgc aaacaaagca tacgaacctt
    23401 tagtaaaatc atttccatga aactagaata gagccctctt agcaaaaatc attattttaa
    23461 tttatttcta atcactcctt gacataaata actctcacca ataaaagact atgtcttaaa
    23521 aaaatggtat aataaaatca atacttgggc ttgatggcta tgctactaat aacaattagg
    23581 agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa aggaacttta
    23641 gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt caaaaacaaa
    23701 tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct gctcaagatc
    23761 ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc aatccagctg
    23821 tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc atcaactctg
    23881 ctcttgaaca aatcgatgaa actaccaaga aaaaactggg tgctttcgct gggaaattac
    23941 ctttctaaaa acaaggagct agaacaatgc ttgtcgataa caaaggctaa gaaaggtgca
    24001 aaaatg
//
LOCUS       DBERYS010000029        23990 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.029, whole genome shotgun sequence.
ACCESSION   DBERYS010000029 DBERYS010000000
VERSION     DBERYS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.029"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..661
                     /locus_tag="ACL8LA_001323"
     CDS             <1..661
                     /locus_tag="ACL8LA_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813076.1"
                     /translation="FYLNKGKVYGFVGPNGAGKTTIIKMILGILKPDSGKITIFNQTV
                     EQNSENILSRIGLVLGPSFYGHLDAYKNLKLIANMKGLSLDTERLNEYLSMVGLKDVK
                     KKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPINGLDPQGVIEIRSLIRFLQEKKGI
                     TFLISSHILSELDKVISDIIIINYGKVEFFGSCHYLLQKYNCRNLEEAYLACLAGGEY
                     D"
     gene            654..1391
                     /locus_tag="ACL8LA_001324"
     CDS             654..1391
                     /locus_tag="ACL8LA_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813077.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNVLLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            1404..2114
                     /locus_tag="ACL8LA_001325"
     CDS             1404..2114
                     /locus_tag="ACL8LA_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813078.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISALYSE
                     LDYNKYWYILHTLIYMLMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIFMQICCI
                     RFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVCIWIMG
                     FEFFLLPKLIKLKEQNLV"
     gene            2656..2877
                     /locus_tag="ACL8LA_001326"
     CDS             2656..2877
                     /locus_tag="ACL8LA_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1813079.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3323..3647
                     /locus_tag="ACL8LA_001327"
                     /pseudo
     CDS             3323..3647
                     /locus_tag="ACL8LA_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3775..5004
                     /locus_tag="ACL8LA_001328"
     CDS             3775..5004
                     /locus_tag="ACL8LA_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS1813080.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5024..7720
                     /gene="ppc"
                     /locus_tag="ACL8LA_001329"
     CDS             5024..7720
                     /gene="ppc"
                     /locus_tag="ACL8LA_001329"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGS1813081.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    7803..8020
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8133..9137
                     /gene="trpX"
                     /locus_tag="ACL8LA_001330"
     CDS             8133..9137
                     /gene="trpX"
                     /locus_tag="ACL8LA_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1813082.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9206..10072
                     /locus_tag="ACL8LA_001331"
     CDS             9206..10072
                     /locus_tag="ACL8LA_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813083.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10069..10827
                     /locus_tag="ACL8LA_001332"
     CDS             10069..10827
                     /locus_tag="ACL8LA_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813084.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            10917..12677
                     /gene="dnaG"
                     /locus_tag="ACL8LA_001333"
     CDS             10917..12677
                     /gene="dnaG"
                     /locus_tag="ACL8LA_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGS1813085.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12680..13789
                     /gene="rpoD"
                     /locus_tag="ACL8LA_001334"
     CDS             12680..13789
                     /gene="rpoD"
                     /locus_tag="ACL8LA_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGS1813086.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            13803..14132
                     /locus_tag="ACL8LA_001335"
     CDS             13803..14132
                     /locus_tag="ACL8LA_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS1813087.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14247..15290
                     /gene="cpoA"
                     /locus_tag="ACL8LA_001336"
     CDS             14247..15290
                     /gene="cpoA"
                     /locus_tag="ACL8LA_001336"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGS1813088.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15306..16631
                     /locus_tag="ACL8LA_001337"
     CDS             15306..16631
                     /locus_tag="ACL8LA_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1813089.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16662..16838
                     /locus_tag="ACL8LA_001338"
     CDS             16662..16838
                     /locus_tag="ACL8LA_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS1813090.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            16897..17031
                     /locus_tag="ACL8LA_001339"
     CDS             16897..17031
                     /locus_tag="ACL8LA_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGS1813091.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17095..18405
                     /gene="obgE"
                     /locus_tag="ACL8LA_001340"
     CDS             17095..18405
                     /gene="obgE"
                     /locus_tag="ACL8LA_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGS1813092.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18415..18573
                     /locus_tag="ACL8LA_001341"
     CDS             18415..18573
                     /locus_tag="ACL8LA_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813093.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            19660..20919
                     /locus_tag="ACL8LA_001342"
     CDS             19660..20919
                     /locus_tag="ACL8LA_001342"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS1813094.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            20935..21486
                     /locus_tag="ACL8LA_001343"
     CDS             20935..21486
                     /locus_tag="ACL8LA_001343"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813095.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            21479..22756
                     /gene="spxR"
                     /locus_tag="ACL8LA_001344"
     CDS             21479..22756
                     /gene="spxR"
                     /locus_tag="ACL8LA_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGS1813096.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            22772..23632
                     /locus_tag="ACL8LA_001345"
     CDS             22772..23632
                     /locus_tag="ACL8LA_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGS1813097.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            23857..>23990
                     /locus_tag="ACL8LA_001346"
     CDS             23857..>23990
                     /locus_tag="ACL8LA_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS1813098.1"
                     /translation="MSQSIVPVEIPQYCRFDSKKRNGILFNVRIANLKFTFFRLDFLR
                     "
BASE COUNT         7444 a         3986 c         5189 g         7371 t
ORIGIN      
        1 attttattta aataagggaa aagtatatgg ttttgttgga ccaaatgggg ctggaaaaac
       61 tacaattatc aaaatgattc tagggatttt aaaaccagat tctgggaaaa taactatttt
      121 taatcaaacc gtagaacaga attccgaaaa tatattatct aggataggat tagttttggg
      181 accttcattt tatggtcatc ttgatgctta taaaaatttg aagttaatag caaatatgaa
      241 agggcttagt ttagatactg aaagacttaa tgaatatcta tcaatggtag gattaaaaga
      301 tgttaaaaag aaaaaagtga aaaatttttc aatgggcatg aaacaacgtc taagtatagc
      361 tgcatcattg ttaggtagtc cagaaatatt aatttgggat gaaccaatca atggacttga
      421 tccacagggt gtaattgaaa ttagaagttt aatccgtttt ctacaggaga aaaaaggtat
      481 tacattttta atatcaagtc atattcttag tgaactggat aaagtgatat ctgatattat
      541 aattataaat tatgggaaag ttgaattttt tggttcttgc cactaccttt tacaaaagta
      601 taattgtcga aatctggaag aagcctactt agcttgttta gctggaggtg aatatgatta
      661 aattagagtt tctaaagcag aaaaaaagca tcttatggtt tgttcttatt tttccaataa
      721 ttctgaatgt tttgttatat atagacttaa cttttaggta tcgaggttat ttattggtgc
      781 atcaaaatga acttgcttta tctaattggc agttaatttt taaagaacaa acaatatttt
      841 atttttctga actattttat ttagttctat ctttaataat ttacgaagtg tttgcagtag
      901 aatttaaaaa tgatgcatgg ttaaccgtaa tttcattgcc atttagaaac aagtacacaa
      961 taaattctaa attactcatt acagtggttt acacatttac tttttggttg tctgattata
     1021 ttagcttata cgttattggg aaggctattg ataacagttt agagattggt ttgattttct
     1081 ttttaaaaac atttaccatt caattaattt cctcgttgat gataatgtta ttatattttc
     1141 taactttagt acttataagg aaaatttctg gaataatacc aattggtatt ataatgatga
     1201 ttttaacaat tagcatatac tacaacgatt ataattttaa gatttactta ccatttactt
     1261 atctttctca tgcttttaga gtgactgaaa gtcaatttta tatgatttta ctatctaata
     1321 taataatcat cgtacttttt tatatattaa ttcgtaaatt gaacgaaaga agttttgaga
     1381 tgaaacttta ataggtaata aaaatgaagt tacttaaaaa tgaattgatt aagagtaaaa
     1441 tatttttatt tattattgtt gacatctgta ttcagattct agtcattcta gctataaaaa
     1501 catatatttt agatatttcg gcactatact cagagcttga ttataataag tattggtata
     1561 ttttacacac tttaatatat atgctaatga tttttcctat acaaattttg tatcaaaatc
     1621 taagagaggc attgattgaa gataataata atggttggaa tatcatggtg attaacacta
     1681 ataatctcgt gaaaatcatc tatataaaag taacgataaa tatcgtgaga tgttttattt
     1741 gttattttgt ttatacaatt ttttctttaa ttcaattagg tggtatggga actgatatgt
     1801 tattaaccaa tataatattt cccaatataa tgtcatttct tttattttta cctatagcta
     1861 tctttatgca aatttgctgt ataagatttg attccatttt agcaaaggcc ttacctaata
     1921 ttttgctgat acttattgtt ttaatcacgt ttcagagtga ttggaatata tttatacctg
     1981 caacttatta ttatacagaa attcagtcaa ctacaaactt aggtattaaa ttattagttt
     2041 gtatctggat tatgggattt gaatttttcc ttcttccaaa actaataaaa ttgaaagaac
     2101 aaaatctagt ttaaatgttg ctcgaaaatt aaataattga atacgattaa caatgaaaca
     2161 atacaacgtg agtcatgact tcaaagtaat tgattttatg attctggttt cttacgctta
     2221 attaagcaag ttgtattcac tttatcaaat tgttattact ctaaaaagcg tctaacagct
     2281 atagagtgtc tgtttgcttc tctttacttt tattttaaat aattcattaa ccttccgtaa
     2341 gtgcccaata ataaggtgtg ttgaagaagt ctgagataaa atagttctca gtcacaaaaa
     2401 agctatagat tcccattggt gaacagctcc ctgtcaagta gacagtaaaa taatataaaa
     2461 tcttaagcga cctgattcct gaattccagt ggagtcaggt tgttttttaa ccgtctttct
     2521 taatattgtc ttacctataa tagatatgct tatgagatat gtttaataaa ttaattttag
     2581 ttcaaaatgt ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc
     2641 tcaggagaaa gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc
     2701 caaaaacaaa tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg
     2761 ggtgagcata cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt
     2821 acagactacc tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct
     2881 cctcaatttc atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat
     2941 agtccaaaat ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga
     3001 gcgattttaa aaccatcaat aaaatagagc gatactttat atgccatgat acaaatgata
     3061 tacaatgata cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt
     3121 atgatgactt catcagtcat gccacgttaa atgtgtgagt ttgttagaga aacgcaatta
     3181 atcctcaaaa ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa
     3241 gcttgttgct aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac
     3301 aaaaatacaa tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg
     3361 tttcaagagc cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat
     3421 caacgcaacg taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc
     3481 tcatggtaac tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt
     3541 ttggcattca cgaaagcaac ttaatccgtc ggagccaatg ggttgaagta actcttgttc
     3601 aaaatggttt tacgatttca aattctgccc taattatgaa aaacagtaaa attcgaagga
     3661 ttgtaaggta agagtttttt tctttctgaa aaaatggtat aatagcaatc aaaactagaa
     3721 aataaaacgg aatttggaac agatttgtct gtatcctagt agagtggtga tgctatgaag
     3781 attagtaaga ggcacttatt aaattattcc atcttgattc cctacttgct tttatctatt
     3841 ttgggcttga ttgtggtcta ttcgaccacc agtgctattt taattgaaga aggcaagagc
     3901 gccttgcagt tggttcgaaa ccaaggaatc ttttggattg ttagtttgat actgattgcc
     3961 ttaatttata aattgagact agattttttg agaaatgagc gactaatcat tttagttata
     4021 ttaatagaaa tgcttttatt gttcttggct cgttttattg gtatttccgt aaacggggca
     4081 tacggttgga tttcggttgc aggagtaact attcagccag ctgagtactt aaaaatcatt
     4141 attatttggt atttagctca ccgattctcc aaacagcaag aagaaatagc tacttatgat
     4201 tttcaagttt tgactcaaaa tcaatggctt ccccgtgctt ttaatgattg gcgattcgtt
     4261 ctcctagttc tgattggaag tttgggaatt ttccctgatt taggaaatgc gactatttta
     4321 gtcttggttt ccttgattat gtatacagtt agtggaatcg cttatcgctg gttttcaacc
     4381 attctggcgc tcgtatctgc cgcttctgtc tttgtcttga ccactatcag cctaatcggt
     4441 gttgagacct tttcaaaaat tccagtattt ggctatgtag ccaagcgctt tagtgccttt
     4501 tttaatcctt ttgccgatcg tgctgatgca ggtcaccagt tagctaattc ttattttgcc
     4561 atggtcaatg gtggttggtt tggtctaggt cttggaaact cgattgaaaa acgaggttat
     4621 ttgccagaag ctcatacaga ctttgtcttt tctatcgtga ttgaagaatt tggctttgct
     4681 ggtgccagtc ttattttagc tctcttgttt ttcatgattt tgcggattat cttggtcggt
     4741 attcgagcgg agaatccttt caatgccatg gttgcactcg gtgtcggagg gatgatgttg
     4801 gttcaggtat ttgtcaatat cggagggatt tcgggcttga ttccatctac aggagtaacc
     4861 ttccccttct tatcccaggg tggaaatagt cttctagtct tatcagtggc agtagccttt
     4921 gtcttaaata ttgatgccag tgaaaaacgc gctaagttgt accgagaatt ggaaaatcaa
     4981 ccaatgaacc ttctgttgaa gtaggataaa gaaaggatag tttatgtctc ttcaaaaatt
     5041 agaaaattat agtaataaaa gtgttgtgca agaagaagtc ttgattctaa cagaattact
     5101 ggaagatatt actaaaaata tgcttgcccc agagaccttt gaaaaaataa tacagttgaa
     5161 agaattatca acgcaggaag attatcaagg tctaaaccgt ctagtgacta gcttatcaaa
     5221 tgatgaaatg gtctatattt cacgctattt ctctatctta cctcttttga tcaatatttc
     5281 agaggatgtg gatttagctt atgaaatcaa tcatcaaaat aatattgatc aggactattt
     5341 aggtaaatta tctacaacga ttaaattggt agcagaaaag gaaaatgccg ttgagatcct
     5401 agaacacttg aatgttgtcc ctgttttgac agcccatcca acacaagtgc aacgcaaaag
     5461 tatgttggat ttaacaaatc atattcatag tcttttgcgt aaataccgtg atgttaagtt
     5521 ggggttgatc aataaagata aatggtacaa tgatttgcgt cgttacatcg aaattatcat
     5581 gcagacagac atgattcgtg agaaaaaatt aaaagtgact aacgaaatca cgaatgctat
     5641 ggaatattat aacagctcct ttttgaaagc tgtacctcat ttgacgacgg agtataagcg
     5701 cttagcgcaa gcgcatggtc tgaatttaaa acaggctaaa ccaatcacca tgggtatgtg
     5761 gataggtggt gaccgtgatg gaaatccatt tgttacagca gagaccttga agcagtctgc
     5821 actcactcaa tgtgaagtca tcatgaacta ctatgataaa aagatttacc aactttatcg
     5881 tgaattttct ctttcaacta gcattgtcaa cgtcagcaag caagtcagag aaatggctcg
     5941 tcaatccaag gataactcga tttaccgcga aaaagagctt taccgtcgtg ccttgtttga
     6001 tattcaatca aaaattcagg caactaaaac ctatctgatt gaggatgaag aagttgggac
     6061 tcgttatgaa accgccaatg atttctacaa ggatttgatt gccattcgag attctctact
     6121 agaaaataag ggtgagtcct tgatttcagg tgattttgtg gaattattgc aggcagtaga
     6181 gatatttggt ttttacctag catcaattga tatgcgacaa gactctagcg tctatgaagc
     6241 ctgtgtggca gaactcttga aatcagcagg aattcattct cgttatagcg agttgagcga
     6301 agaagaaaag tgtgaccttc tcttgaaaga attagaagaa gatccccgaa ttctttctgc
     6361 gactcacgca gaaaaatcag aattattagc aaaagaatta gctattttta agacggctcg
     6421 tgttttgaaa gataagttgg gagatgatgt catccgtcag accatcattt cacatgcaac
     6481 cagcctttct gatatgctag aattagctat tctgttaaaa gaagtaggac tggtggatac
     6541 ggaaagggcg cgtgttcaga ttgttcccct ttttgaaaca attgaagact tggatcattc
     6601 agaagaaaca atgagaaaat atctttctct tagccttgcc aaaaaatgga ttgactcacg
     6661 aaataactac caagaaatca tgcttggcta ctctgacagt aataaagatg gcggttactt
     6721 gtcatcatgt tggaccctct acaaggctca acaacaattg actgctattg gagatgaatt
     6781 tggcgttaag gttaccttct tccatggtcg tggtggtact gtcggtcgtg gtggtgggcc
     6841 aacctatgaa gccattacat ctcaaccgct caagtctatc aaggatcgta tccgcttgac
     6901 ggagcagggt gaagtaattg ggaataaata cggtaacaaa gacgccgctt actataacct
     6961 tgaaatgcta gtatcggcag ctattaaccg tatgattact cagaagaaga gcgataccaa
     7021 taccccaaat cgttatgaag ccattatgga tcaagtagtg gaccgtagtt acgatatcta
     7081 ccgtgatttg gtctttggta atgagcattt ctatgattat ttcttcgagt caagtccaat
     7141 caaggctatt tcaagtttta atattggttc tcgtccagcc gctcgtaaga ctattactga
     7201 aatcggtggt ttgcgtgcca tcccttgggt attctcatgg tcacagagtc gtgttatgtt
     7261 ccctggatgg tacggggttg gttcaagctt caaggaattt atcaataaaa atccagagaa
     7321 tattgctatc ttacgagata tgtaccaaaa ttggcctttc ttccaatcgc ttctttcaaa
     7381 tgttgatatg gttttgtcaa aatcaaatat gaatattgct tttgaatatg ctaaactttg
     7441 tgaagacgag caagttaagg ccatctatga gactatttta aatgaatggc aagttactaa
     7501 gaacgttatc ttggctattg aaggacatga cgaactctta gctgacaatc catatctaaa
     7561 agctagtctg gattaccgta tgccttactt taatattctc aactatattc agttagagtt
     7621 gattaaacgc caacgtcgtg gagaattgtc cagtgatcaa gaacgattga ttcatatcac
     7681 catcaacgga attgcgacag gattgcgtaa ttcaggttga taattttcaa gagtgaatgc
     7741 taaaagtgaa tatcaaaaaa attctaatag actattgaca agtagtttaa aaatgatata
     7801 atttaaccat tcagaaaagt aatcatacaa actttttaga gagtctgtgg tagctgaaaa
     7861 cagataagtg gcgatgatga aaattgggct gaatgctatt tagaatttga aattataaaa
     7921 attcggtaag cacaccttac agtgcatctc gttattgcga gactgagcga tagggaaatt
     7981 ccctataatt gaggtggtac cgcgcatcga cgtcctcaca caagtttttt gtgtgaggat
     8041 ttttttgatg gaggttagta tggaaagaaa acgatggcgt cgcttgttta gataagtgaa
     8101 atatgttaaa ggaaataaaa aggagaaaca gaatgaaaaa taaacgttta attggaatta
     8161 ttgctgcatt agcagtctta gtagcaggaa gcttgattta ttcttcaatg aataaatcag
     8221 aagctcagaa taataaggat gagaagaaaa taactaagat tggtgtgctt caatttgtga
     8281 gccatccatc ccttgatttg atttataaag ggatccaaga tggacttgca gaagaaggat
     8341 ataaagatga tcaagttaaa attgatttta tgaactcaga aggtgaccaa agtaaggttg
     8401 cgacaatgag taaacaattg gttgcaaatg ggaatgacct tgtggttggt atcgcaacac
     8461 cagcagccca agggttggct agtgcaacaa aagacctacc ggttatcatg gccgctatta
     8521 cagacccaat tggtgctaac ttggttaaag atttgaaaaa accaggtggc aacattacag
     8581 gggtatctga ccacaatcca gctcaacaac aagttgaact catcaaggct ctgacaccga
     8641 atgtgaaaac aatcggagct ctttactcaa gtagcgaaga caattcaaaa acacaggtcg
     8701 aagaatttaa ggcttatgct gaaaaagcag gtctgacagt ggaaacattt gcagttcctt
     8761 caacaaatga aattgcctca actgtcactg ttatgactag caaggtagat gctatttggg
     8821 ttccaattga taacaccatt gcatcaggat ttccaacggt tgtctctagc aatcaaagtt
     8881 ctaagaaacc aatttatccc agtgcgacag ctatggtaga agtaggtggt ttggcatcag
     8941 ttgtaattga ccaacatgac cttggtgtgg caacaggtaa aatgattgtg caagtcttga
     9001 aaggtgcaaa accagccgat accccagtca atgtcttttc aactggtaag tcagtcatca
     9061 ataaaaaaat agcacaagaa ctaggtatta ctattcctga gtctgttctc aaagaagcag
     9121 gacaagtcat cgaataaata aggtagggag gaggagactc ttcccatttt ttaaaaagat
     9181 gaatatagaa aggattaaat aaaatatgat tgtttccatt atttctcaag gatttgtctg
     9241 ggctattcta ggtctgggaa tctttatgac atttaggatt ttaaactttc cagatatgac
     9301 gacagaaggt tccttccctc ttgggggagc tgttgctgtc actttgataa ccaaaggcgt
     9361 gaacccattt ttagcgacac ttgttgctgt aggagcaggt tgtttggctg gaatggcagc
     9421 aggccttctt tatacaaaag ggaagatccc aaccttgcta tcagggattt tggtgatgac
     9481 ttcttgtcac tcaatcatgc tcttgattat gggacgtgcg aatttaggcc tgcttggaac
     9541 caagcaaatt caggatgttt tgccttttga ttcggatttg aatcaactct tgacaggtct
     9601 catctttgtg agtattgtta ttgctctcat gctctttttc ttggacacta aactcggaca
     9661 agcctatatt gctacagggg ataatcctga tatggctaga agtttcggga ttcatactgg
     9721 acgcatggag ctcatgggct tggtcttatc aaatggtgtg attgcccttg caggtgccct
     9781 cattgctcag caagaaggtt atgccgatgt gtctcgaggg atcggggtta tcgttgtggg
     9841 gcttgcaagt ttgattattg gagaagttat tttcaagagt ttgagcttgg cagagcgttt
     9901 ggttactatc gttgtaggtt ctatcgctta tcaattttta gtgtgggcag ttatcgcact
     9961 tggctttaat acaagttacc ttcgtttata cagtgccttg attttagcag tctgcctcat
    10021 gattccaaca tttaagcaaa caatcttgaa aggagccaag ttaagcaaat gacagcaatt
    10081 gtagaattaa aaaatgcaac caaaatcgtt aaaaatggct ttgatgaaga aaagattatt
    10141 ttaaatgatg tttccttaga aatttttgaa cgggacttta tcacgatttt gggcggaaat
    10201 ggtgctggaa aatcaaccct ctttaacact atagcaggga ccttatcact aactagtgga
    10261 actatccgta ttttaggtga agaactcact aagttttcac ccgagaagcg tgccaagtac
    10321 ctgtctcgtg tcttccaaga tccaaagatg gggacagctc cccgtatgac ggtcgctgaa
    10381 aatcttttaa tcgccaagtt tcgtggtgaa aagcgtggat tgttaccacg acgcttgact
    10441 agctataagg atgaatttca ggcaaccatt gaaaaagtag gaaatggtct tgagaaacac
    10501 ttgaatacac cgattgagtt cttatcaggt ggacaaagac aggctttgag tctcttgatg
    10561 gcaaccttga agcgacctga attactcctg ttagatgagc atactgctgc cctggatcca
    10621 aagactagtg ttgctttgat ggaattgaca gatgaatttg ttaagaaaga tcagctaaca
    10681 gcccttatga ttactcatca tatggaagat gctctcaaat acggcaatcg cttgattgtc
    10741 atgaaagaag gacggattat ccaagattta aaccaagaag aaaaagcaaa aatgaaaatc
    10801 tctgattatt atcaactctt tgaataaagt agggaaaatg agtgaaatgg tttacttttt
    10861 ttctgaaata aagtatacta tataaagtaa actatgataa catggaggta ttgtgtatgg
    10921 ttgacaaaca agtcattgaa gaaatcaaaa acaatgccaa cattgtggaa gtcataggag
    10981 atgtgatttc tttacaaaag gcaggacgga actatctagg gctctgtcct tttcatggtg
    11041 aaaaaacacc ttctttcaac gttgtagagg ataagcagtt ttatcactgt ttcggttgtg
    11101 gtcgctcagg tgatgtcttt aagttcatcg aggagtacca aggggttccc tttatagagg
    11161 cagtccaaat cttaggtcag cgtgttggaa ttgaggttga aaaaccgctt tatagtgagc
    11221 agaagccagc ctcacctcac caagctcttt atgatatgca cgaagatgca gccaaatttt
    11281 accatgcgat tctcatgaca acgactatgg gcgaagaggc cagaaattac ctttatcagc
    11341 ggggtttgac agatgaagtg cttaaacatt tttggattgg tttagcacct ccagaacgaa
    11401 actatctcta tcaacgtttg tctgatcagt atcgtgaaga ggatttactg gattcaggcc
    11461 tgttttatct ctcggatgcc aatcaatttg tagacacctt tcacaatcgc attatgtttc
    11521 ccctgacaaa tgaccaagga aaggtcattg ccttctcagg tcgtatctgg caaaaaacgg
    11581 attcacaaac ttctaagtat aaaaacagcc gatcgactgc aatttttaac aaaagttacg
    11641 aattatatca tatggatagg gcaaaaaaat cttctggaaa agctagtgag atttacttga
    11701 tggaaggatt catggatgtt attgcagcct atcgggctgg aatcgaaagt gctgtggcgt
    11761 cgatgggaac ggccttgagt cgagagcatg ttgagcatct gaaaaggtta accaagaaat
    11821 tggttcttgt ttacgatgga gataaggctg ggcaagccgc gacattgaaa gcattggatg
    11881 aaattggtga tatgcctgtg caaatcgtca gcatgcctga taacttggat cctgatgagt
    11941 atctacaaaa aaatggtcca gaagacttgg cctatctatt aacgaaaact cgtattagtc
    12001 cgattgagtt ctacattcat cagtacaaac ctgaaaacag tgaaaatctg caggctcaga
    12061 ttgagtttct tgaaaaaata gctcccttga ttgttcaaga aaagtccatc gctgctcaaa
    12121 acagctatat tcatatttta gctgacagtc tggcgtcctt tgattatacc cagattgagc
    12181 agattgttaa tgagagtcgt caggtgcaaa ggcagaatcg catggaagga atttccagac
    12241 cgacgccaat caccatgcca atcaccaagc agttatcggc tattatgagg gcagaagccc
    12301 atctactcta tcggatgatg gaatcccctc ttgttttgaa cgattaccgt ttgcgagaag
    12361 actttgcatt tgctacacct gaatttcagg tcttatatga cttgcttggc cagtatggaa
    12421 atcttcctcc agaagtttta gcagagcaga cagaggaagt tgaaagagct tggtaccaag
    12481 ttttagctca ggatttgcct gctgaaatat cgccgcagga acttagtgaa gtagagatga
    12541 ctcgaaacaa ggctctcttg aatcaggaca atatgagaat caaaaagaag gtgcaggaag
    12601 ctagccatgt aggagataca gatacagccc tagaagaatt ggaacgttta atttcccaaa
    12661 agagaagaat ggagtaataa tggcaacaaa acaaaaagaa gtaacaacat ttgacgtaca
    12721 ggtagcagaa tttatccgta atcataagca aaaagggaca gcaacagatg atgaaatcaa
    12781 tgcaagtctg gttattcctt ttaccttgga cgctgatggg attgaagatc tcttgcaacg
    12841 gattcaggat gcaggaattt ctatcacaga taacgaagga aatccaagtg cgcgtgttct
    12901 tagcaatgaa gaagaaccag aactcagcga tgaggacttg attgggtcaa cttctgctaa
    12961 ggtcaatgac cctgtccgta tgtacttgaa agaaataggg gtcgttcctc tcttgaccaa
    13021 tgaagaggag aaagagttgg cactggctgt tgaagctggt gatatcgaag ccaaacaacg
    13081 tcttgcggaa gccaatcttc gtttggttgt ttccattgcc aaacgctatg tcggtcgtgg
    13141 catgcagttc cttgacttga ttcaagaagg aaatatgggc ttgatgaagg cggttgacaa
    13201 gtttgactat tctaaagggt tcaagttttc aacttatgca acttggtgga ttcgtcaggc
    13261 tatcactcgt gctattgcgg accaagctcg taccatccgt atcccagttc acatggttga
    13321 aactatcaat aaattggttc gtgaacagcg gaatctcctt caagaattgg ggcaagatcc
    13381 gacaccagaa cagattgctg aacgaatgga tatgacacct gataaggttc gtgaaatctt
    13441 gaagattgcc caagaaccag tatctcttga aactcctatc ggtgaagagg acgatagcca
    13501 ccttggagac tttatcgaag atgaagtgat tgaaaatcca gtggattata cgactcgtat
    13561 cgtcttgcgt gagcaattgg atgaaatctt agatactctt acagaccgtg aagaaaatgt
    13621 tctgcgtcta cgttttggac tagatgatgg aaaaatgcgc acacttgaag atgtggggaa
    13681 agtctttaac gtaactcgtg agcgtatccg tcagattgaa gcaaaggctt tgagaaaact
    13741 acgccaacca agtcgtagca aaccgcttcg tgattttatt gaagactaag agtgaggaaa
    13801 atatggctta tacagaagag caaattgaaa acatcaaaac acggatttta acagccttgg
    13861 aagaagtcat cgaccctgag ttgggaatcg atattgtcaa tcttggtttg atctatgaga
    13921 ttcgttttga cggtgacaca gggcaaacag agattgacat gactttgaca actatgggtt
    13981 gtcccttagc agaccttttg acagaccaga tttatgatgc tatgatagag gttccagaag
    14041 taacggatac tgaggttaaa ttagtctggt atccagcctg gacagttgaa aaaatgagtc
    14101 gctatgcccg cattgcccta ggcattaagt aaacatataa aagcatgtga gagactgttg
    14161 gaaaagcgag gaaatttccc ctcttttcct ctagtctctc ctttcttttg ctgattttat
    14221 tcaaagaaaa tgatataata gtagttatgg agaaaaagaa attacgcatc aatatgttga
    14281 gttcaagtga gaaagtagca ggacagggag tttcaggtgc ttaccgtgaa ttagttcgtc
    14341 ttcttcaccg tgctgccaag gaccaattga ttgttacaga aaatcttcca atcgaggcag
    14401 atgtgactca ctttcatacg attgattttc cctattattt atcaaccttc caaaagaaac
    14461 gctcagggag aaagattggc tatgtgcatt tcttgccagc tacacttgag ggaagtttga
    14521 aaattccatt tttcttaaag ggaattgtga aacgctatgt attttctttt tacaaccgga
    14581 tggagcactt ggttgtggtc aatcctatgt ttattgagga tttggtagca gctggtattc
    14641 cacgtgaaaa agtgacctat attcctaact ttgtcaacaa ggaaaaatgg catcctctac
    14701 cacaagaaga ggtagtcaga ctgcgcacag atcttggtct tagtgacaat cagtttatcg
    14761 tagtaggtgc tgggcaagtt cagaaacgta aagggattga tgactttatc cgtctggctg
    14821 aggaattgcc tcagattacc tttatctggg ctggtggctt ctcttttggt ggtatgacag
    14881 atggttatga acactataag aaaattatgg aaaatccccc taaaaatttg atttttccag
    14941 gcattgtatc gccagagcgg atgcgcgaat tgtatgctct agcggatctt ttcttgttgc
    15001 ctagttacaa tgagctcttt cctatgacta ttttagaagc tgcgagttgt gaggctccta
    15061 ttatgttgcg tgatttagat ctctataagg taattttgga gggaaattat cgggcgacag
    15121 cgggtagaga agagatgaaa gaggctattt tggaatatca agcaaatcct gctgccttaa
    15181 aagatctcaa agaaaaggct aagaatattt ccagagagta ttctgaagag catctgttac
    15241 aaatctggtt ggacttttat gagaaacaag ccgctttagg gagaaagtaa aaagtgaggt
    15301 aatctatgcg aattggttta tttacagata cctattttcc tcaggtttct ggtgttgcga
    15361 ccagtattcg aaccttgaaa acagaacttg aaaagcaggg acatgctgtt tttatcttta
    15421 cgacgacaga taaggatgtc aatcgctacg aagattggca aattatccgc attccaagtg
    15481 ttcctttctt tgcttttaag gatcgtcgct ttgcctaccg aggttttagc aaggcacttg
    15541 aaattgctaa acagtatcag ctagatatta tccatactca gacagaattt tctcttggcc
    15601 tgttggggat ttggattgcg cgtgaattga aaattccagt catccatacc tatcacaccc
    15661 agtatgaaga ctatgtccat tatattgcta aggggatgtt gatccggccg agtatggtca
    15721 agtatctggt tagaggtttc ctgcatgatg tggatggggt tatttgtcct agtgagattg
    15781 tccgtgactt gctatctgat tataaggtca aggttgaaaa acgggtcatt cctactggga
    15841 ttgaattagc caagtttgag cgtccggaaa tcaagcagga aaatttgaaa gaactgcgta
    15901 gtaaactagg gattcaagat ggtgaaaaga cgttgcttag tctttcgaga atctcctatg
    15961 aaaaaaatat tcaagcagtt ttagcagcct ttgctgatgt tctgaaagag gaagacaagg
    16021 ttaaactggt agtagctggg gatggccctt atctgaatga cctcaaagag caagctcaga
    16081 acctagagat tcaagactca gtcatcttta cagggatgat tgctcctagt gagacggctc
    16141 tttactataa agcggcggat ttcttcattt cggcatcgac aagcgaaacg caaggtttga
    16201 cctacttgga aagcttagcc agtggaacac ctgtcattgc tcacggaaat ccttatttga
    16261 acaacctcat cagtgataaa atgtttggaa ccttgtacta tggagaacat gatttggctg
    16321 gtgctatttt ggaagccctg attgcaacac cagacatgaa cgagcatacc ttatcagaga
    16381 aattgtatga gatttcagct gagaactttg ggaaacgagt gcatgagttt tatctggatg
    16441 ccattatttc aaataacttc cagaaagatt tggctaaaga tgatacggtc agtcagcgta
    16501 tctttaagac agttttgtat cttcagcaac aggtggttgc tgtacctgta aaaggatcta
    16561 gacgcatgtt gaaggcttca aaaacacagt tgatcagtat gagagactat tggaaagacc
    16621 atgaagaata gaaagaggaa cagctatgaa aaaaacaatt aatgagaagc ggtcgtgata
    16681 aaaagattgc gggtgtttgt gctggggtgg cccattatct ggatatggat ccgactatcg
    16741 ttcaagtcat ttggggtgtt cttacttgct gttacggagc tggaattgta gcttacatta
    16801 ttttatggat tatcgcacca gtagcaactg actattgaaa actagctcaa ttcatgctaa
    16861 gataatagaa aatagaatgt aacgaaggag agaaaaatgg catttggaga taatggaaat
    16921 cgtaaaaaaa ctatgtttga gaaaataacc ttgtttatcg tgattatcat gctagtagca
    16981 agtttattgg gaatttttgc aactgcaatt ggtgccctca gtaatctata aaatagattc
    17041 aagaaaattt agtgactggg atttcccagc ccttttttaa agtgagaaga aataatgagt
    17101 atgtttttag atacagctaa gattaaggtc aaggctggta atggtggcga tggtatggtt
    17161 gcctttcgtc gtgaaaaata tgtccctaat ggaggccctt ggggtggtga tggtggtcgt
    17221 ggaggcaatg tggtcttcgt tgtagacgaa ggactacgta ccttgatgga tttccgctac
    17281 aatcgtcatt tcaaggctga ttctggtgaa aaagggatga ccaaagggat gcatggtcgt
    17341 ggtgctgagg accttagagt tcgagtacca caaggtacga ctgttcgtga tgcggagact
    17401 ggcaaggttt taacagattt gattgaacat gggcaagaat ttatcgttgc ccacggtggt
    17461 cgtggtggac gtggaaatat tcgtttcgcg acaccaaaaa atcctgcacc ggaaatctct
    17521 gaaaatggag aaccaggtca ggaacgtgag ttacaattgg aactaaaaat cttggcagat
    17581 gtcggtttag taggattccc atctgtaggg aagtcaacac ttttaagtgt tattacctca
    17641 gctaagccta aaattggtgc ctaccacttt accactattg taccaaattt aggtatggtt
    17701 cgcacccaat caggtgaatc ctttgcagta gccgacttgc caggtttgat tgaaggggct
    17761 agtcaaggtg ttggtttggg aactcagttc ctccgtcaca tcgagcgtac acgtgttatc
    17821 cttcacatca ttgatatgtc agctagcgaa ggccgtgatc catatgagga ttacctagct
    17881 atcaataaag agctggagtc ttacaatctt cgcctcatgg agcgtccaca gattattgta
    17941 gctaataaga tggacatgcc tgagagtcag gaaaatcttg aagaatttaa gaaaaaattg
    18001 tctgaaaatt atgatgaatt tgaagagtta ccagctatct tcccaatttc tggattgacc
    18061 aagcaaggtc tggcaacact tttagatgct acagctgaat tgttagacaa gacaccagaa
    18121 tttttgctct acgacgagtc cgatatggaa gaagaagctt actatggatt tgacgaagaa
    18181 gaaaaagcct ttgaaattag tcgtgatgac gatgcgacat gggtactttc tggtgaaaaa
    18241 ctcatgaaac tctttaatat gaccaacttt gatcgtgatg aatctgtcat gaaatttgcc
    18301 cgtcagcttc gtggtatggg ggttgatgaa gcccttcgtg cgcgtggagc taaagatggg
    18361 gatttggtcc gcattggtaa atttgagttt gaatttgtag actaggagac tggtatggga
    18421 gataaaccga tatctttccg agatgcggat ggtaattttg tttccgccgc agacgtttgg
    18481 aatgaaaaga aattggaaga actatttaat cgtctcaatc caaatcgtgc cttgagattg
    18541 gcacgaacta aaaaggaaaa tccatctcag taaagaagct aaaaaatccc gtgcctcatc
    18601 agacacggga ttttgtggta cgacaggcat gtatagcaaa ctgaatctgg aatagcacag
    18661 catatcttct aaaatattgc tataaattga tttgaatttc aaaattaaat tgttttattc
    18721 ttatttcaat ttgttatagt atatctgatg tcaaagttct cggcgagtca aatagtgatt
    18781 cccaagcctg actatcgtga ggtagcggat taaaatggtc tggggataga ccgttttaag
    18841 tctgatgctg gaaataagaa ttgtcagaag aagggatagc gaaatcgtgg ctctacgaac
    18901 aggaacgtga taataaggcg tatatagcga ataagagggc ctcaaactct aaagtccaaa
    18961 aaggtagtcg taacctatat gcgtaaatca tgagagtaat tgaattcgta ctaagatttt
    19021 ctatttttac tgtaaccttt taacgtcctc atatcttgta tacacgagga aagatgtacg
    19081 acttatcccg tgaggtctat cactataaag agaaaacgac agatagaagt gatcctgagt
    19141 cacggttatc tttaaaatag attgtctgat aggatgctag cctgaaaacg tcttgttcat
    19201 gaaatatagg acggtatgta taaaacgctt ctgtgaactg agagaagggg gagaagttct
    19261 tgctaaaatt tagttgaaca gccgtattcc gatacttaga taagagatct agtcttagct
    19321 cctactcagt ttcaggggat aaaaaagggg caatagcgat tcgagaaaga ttatactctt
    19381 cgaaaatctc ttcaaatcac gtcaatatcg ccttgccgta tgtgtaggat actaactacg
    19441 tcagttccat ctacaatctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
    19501 tgatctttga tttttattga gtattagtaa ttcagttact aactcgtcaa ctctgattta
    19561 tccaataaaa ttgaaaagga tggaaaaaag gataaattta tgatatactt tattttgaag
    19621 accttattag aaatcttgaa agagtattga aaacttagaa tgagaaaaat tgttatcaat
    19681 ggtggattac cactgcaagg tgaaattact attagtggtg ctaaaaatag tgttgtggcc
    19741 ttaattccag ctattatatt ggctgatgat gtggtgactt tggattgtgt tccagatatt
    19801 tcggatgtag ccagtcttgt cgaaatcatg gaattaatgg gagctactgt taagcgttat
    19861 gacgatgtct tggagattga tccaagaggt gttcaaaata ttccaatgcc ttatggtaaa
    19921 attaacagtc ttcgtgcatc ttactatttt tatgggagcc tcttaggccg ttttggtgaa
    19981 gcgacagttg gtctaccggg aggatgtgat cttggtcctc gtccgattga cttacacctt
    20041 aaggcgtttg aagctatggg tgccactgct agctacgagg gagataacat gaagttatct
    20101 gctaaagata caggacttca tggtgcaagt atttacatgg atacggttag tgtgggagca
    20161 acgattaata cgatgattgc tgcggttaaa gcaaatggtc gtactattat tgaaaatgca
    20221 gcccgtgaac ctgagattat tgatgtagct actctcttga ataatatggg tgcccatatc
    20281 cgtggggcag gaactaatat catcattatt gatggtgttg aaagattaca tgggacacgt
    20341 catcaggtga ttccagaccg cattgaagct ggaacatata tatctttagc tgctgcagtt
    20401 ggtaaaggaa ttcgtataaa taatgttctt tacgaacacc tggaagggtt tattgctaag
    20461 ttggaagaaa tgggagtgag aatgactgta tctgaagaca gcatttttgt cgaggaacag
    20521 tctaatttga aagcaatcaa tattaagaca gctccttacc caggctttgc aactgatttg
    20581 caacaaccgc ttacccctct tttactaaga gcgaatggtc gtggtacaat tgtcgatacg
    20641 atttacgaaa aacgtgtaaa tcatgttttt gaactagcaa agatggatgc ggatatttcg
    20701 acaacaaatg gtcatatttt gtacacgggt ggacgtgatt tacgtggggc cagtgttaaa
    20761 gcgaccgact taagagctgg ggctgcacta gtcattgctg ggcttatggc tgaaggtaaa
    20821 actgaaatta ccaatatcga gtttatctta cgtggttatt ctgatattat cgaaaaatta
    20881 cgtaatttag gagcggatat tagacttgtt gaggattaaa ccgtagaggt gtttatgaat
    20941 atttggacca aattagcaat gttttctttt tttgaaacgg atcgcttgta tttgcgtcct
    21001 ttctttttta gtgatagtca ggacttccgc gagatagctt caaatccaga aaatcttcaa
    21061 tttattttcc caacgcaggc aagtctggaa gaaagtcaat atgcactggc caattacttt
    21121 atgaagtccc ctttgggagt gtgggcaatt tgtgaccaga aaaatcaaca aatgattggt
    21181 tctattaaat ttgagaagtt agatgaaatc aaaaaagaag ctgagcttgg ctattttttg
    21241 agaaaagatg cttggtcgca aggatttatg acagaggttg ttagaaaaat ttgtcagctt
    21301 tcttttgagg aatttggctt aaaacaatta tttatcatta cccaccttga aaatgaagct
    21361 agccaaagag ttgctcttaa gtctggattt agtttgttcc gtcagtttaa gggaagtgat
    21421 cgttacacaa gaaaaatgcg ggattatctt gaatttcggt atgtaaaagg agagttcaat
    21481 gagtaagcat caggaaattc taagctattt ggaggaatta ccagtaggta aaagggtcag
    21541 tgttcgtagc atttcgaatc atctaggagt tagtgatgga acagcctatc gagctattaa
    21601 agaagctgaa aaccgtggaa ttgtggagac ccgtcctaga agtggaacaa ttcgtgttaa
    21661 atcccagaaa gttgctatag agagattaac gtttgctgaa attgcagaag tgacttcttc
    21721 tgaggttctg gctgggcaag aaggtttaga gagagagttt agtaagtttt caattggtgc
    21781 catgactgaa caaaatatct tgtcttacct tcatgatggg gggctcttga ttgtcggaga
    21841 ccgaacccgt attcagttgc tagccttgga aaatgaaaat gcagttctgg ttacaggggg
    21901 atttcaggtt catgatgatg tgcttaaact ggccaatcaa aaagggattc ctgttctaag
    21961 aagtaagcat gataccttta ccgtcgcgac catgatcaat aaagccttgt caaatgtcca
    22021 aatcaagact gatattctga cagttgagaa actttatcgc cctagtcatg agtatggttt
    22081 tctgagagag acagatacag ttaaagatta tttggacttg gttcgtaaga atcgtagcag
    22141 ccgtttccct gttatcaatc aacatcaggt cgttgttggt gttgtaacca tgagagacgc
    22201 tggtgataaa tcaccaagca cgacaattga taaggttatg tctcgtagtc tattttgggt
    22261 tggattatcg acaaatattg ccaatgtgag tcaacggatg atcgcagaag actttgaaat
    22321 ggtaccagtt gttcgaagca atcaaacttt gcttggcgtt gtgacgcgac gagatgtcat
    22381 ggagaagatg agccgttccc aagtttcggc tctaccaact ttttctgagc agattggaca
    22441 aaagctctct tatcaccatg atgaagtagt cattacagtg gaacccttta tgctagaaaa
    22501 aaatggagtt ttggctaatg gtgtattggc agaaattctg acccacatga cccaagattt
    22561 agttgttaat agtggtcgca atctcattat cgagcagatg ctgatctact ttttgcaggc
    22621 tgttcagata gatgatatat tgcgcattca ggcacggatt attcatcata cgagacggtc
    22681 agctataatt gattacgata tttatcatgg tcaccagatt gtttcaaaag caaatgtgac
    22741 tgttaaaatt aattagaaac taggagaaaa gatgataaca ttaaaatcag ctcgtgaaat
    22801 cgaagctatg gacaaggctg gtgattttct agcaagtatt catataggct tacgtgattt
    22861 gattaagcca ggcgtagata tgtgggaagt tgaagaatat gtccgccgtc gttgtaaaga
    22921 agaaaatttc cttccacttc agattggggt tgacggtgcc atgatggact atccttatgc
    22981 tacctgttgc tctcttaacg atgaagtggc tcacgctttc cctcgtcatt atatcttgaa
    23041 agatggtgat ttgctcaaag ttgatatggt tttgggaggt cccattgcta aatccgacct
    23101 gaatgtctca aaattaaact tcaacaatgt tgaacaaatg aaaaaataca ctcagagcta
    23161 ttctggtggt ttagcagact catgttgggc ttatgctgtt ggtacaccgt ccgaagaagt
    23221 caaaaacttg atggatgtaa ccaaagaagc tatgtacaag ggtattgagc aagctgttgt
    23281 tggaaatcgt atcggtgata tcggtgcggc tattcaagaa tacgctgaaa gtcgtggtta
    23341 cggtgtagtg cgtgatttgg ttggtcatgg tgttggccca actatgcacg aagaaccaat
    23401 ggttcctaac tatggtattg caggtcgtgg actccgtctt cgtgaaggaa tggtcttaac
    23461 cattgaacca atgatcaata caggcgattg ggaaattgat acagatatga aaactggttg
    23521 ggcgcataag accattgacg gtggattgtc atgtcagtat gaacaccaat ttgtcattac
    23581 gaaagatgga cctgttatct tgactagcca aggtgaagaa ggaacttatt aataaaaagt
    23641 gaaaagacta ctggaagttt attttgataa aaaatccagt agatcttttc ataataaaac
    23701 gcattgtatc aagtgttagg agctgatatc atgcgttttt ctgcttttaa gattttttcc
    23761 aactctgttt gtaagcgcat cataacaaag ggtctaggat tcagggctct cctcctatat
    23821 actattagta aagtaaaact aagagaggat attttagtgt cgcagtctat tgttcctgta
    23881 gagattccac aatattgtcg ttttgattct aaaaagagaa atggaattct gtttaacgtt
    23941 cgtattgcca atcttaaatt tacctttttt agattagact tcctgcgaaa
//
LOCUS       DBERYS010000030        23964 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.030, whole genome shotgun sequence.
ACCESSION   DBERYS010000030 DBERYS010000000
VERSION     DBERYS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.030"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            68..353
                     /locus_tag="ACL8LA_001347"
                     /pseudo
     CDS             68..353
                     /locus_tag="ACL8LA_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            457..1245
                     /locus_tag="ACL8LA_001348"
     CDS             457..1245
                     /locus_tag="ACL8LA_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1813099.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPKMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            1276..1794
                     /gene="rimM"
                     /locus_tag="ACL8LA_001349"
     CDS             1276..1794
                     /gene="rimM"
                     /locus_tag="ACL8LA_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGS1813100.1"
                     /translation="MNYFNIGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            1784..2503
                     /gene="trmD"
                     /locus_tag="ACL8LA_001350"
     CDS             1784..2503
                     /gene="trmD"
                     /locus_tag="ACL8LA_001350"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGS1813101.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDDPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            2515..2853
                     /locus_tag="ACL8LA_001351"
     CDS             2515..2853
                     /locus_tag="ACL8LA_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGS1813102.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(2884..3204)
                     /locus_tag="ACL8LA_001352"
     CDS             complement(2884..3204)
                     /locus_tag="ACL8LA_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813103.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            3302..3517
                     /locus_tag="ACL8LA_001353"
     CDS             3302..3517
                     /locus_tag="ACL8LA_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGS1813104.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(3514..3660)
                     /locus_tag="ACL8LA_001354"
     CDS             complement(3514..3660)
                     /locus_tag="ACL8LA_001354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813105.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKAKLMRFEEI
                     FEEY"
     gene            complement(3723..4259)
                     /locus_tag="ACL8LA_001355"
     CDS             complement(3723..4259)
                     /locus_tag="ACL8LA_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGS1813106.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            4685..5023
                     /locus_tag="ACL8LA_001356"
     CDS             4685..5023
                     /locus_tag="ACL8LA_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813107.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            5089..7071
                     /locus_tag="ACL8LA_001357"
     CDS             5089..7071
                     /locus_tag="ACL8LA_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813108.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            7068..7691
                     /locus_tag="ACL8LA_001358"
     CDS             7068..7691
                     /locus_tag="ACL8LA_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813109.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            7688..8188
                     /locus_tag="ACL8LA_001359"
     CDS             7688..8188
                     /locus_tag="ACL8LA_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813110.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(8354..9700)
                     /gene="gor"
                     /locus_tag="ACL8LA_001360"
     CDS             complement(8354..9700)
                     /gene="gor"
                     /locus_tag="ACL8LA_001360"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGS1813111.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            9965..11164
                     /locus_tag="ACL8LA_001361"
     CDS             9965..11164
                     /locus_tag="ACL8LA_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGS1813112.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            11148..11849
                     /locus_tag="ACL8LA_001362"
     CDS             11148..11849
                     /locus_tag="ACL8LA_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813113.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            11851..13110
                     /locus_tag="ACL8LA_001363"
     CDS             11851..13110
                     /locus_tag="ACL8LA_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813114.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            13234..15231
                     /gene="metG"
                     /locus_tag="ACL8LA_001364"
     CDS             13234..15231
                     /gene="metG"
                     /locus_tag="ACL8LA_001364"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGS1813115.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            15281..15424
                     /locus_tag="ACL8LA_001365"
     CDS             15281..15424
                     /locus_tag="ACL8LA_001365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813116.1"
                     /translation="MKIKEQTRKLAAGCSKHSFEVVDETDEVSSKHSFEVVDETDEVS
                     NHT"
     gene            15562..15882
                     /locus_tag="ACL8LA_001366"
     CDS             15562..15882
                     /locus_tag="ACL8LA_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS1813117.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            15886..16701
                     /locus_tag="ACL8LA_001367"
     CDS             15886..16701
                     /locus_tag="ACL8LA_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813118.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGVFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            16776..17702
                     /locus_tag="ACL8LA_001368"
     CDS             16776..17702
                     /locus_tag="ACL8LA_001368"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGS1813119.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            17798..18034
                     /locus_tag="ACL8LA_001369"
     CDS             17798..18034
                     /locus_tag="ACL8LA_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGS1813120.1"
                     /translation="MTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTIEQNGLALEMT
                     PYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            18027..18725
                     /locus_tag="ACL8LA_001370"
     CDS             18027..18725
                     /locus_tag="ACL8LA_001370"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGS1813121.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            18736..19215
                     /locus_tag="ACL8LA_001371"
     CDS             18736..19215
                     /locus_tag="ACL8LA_001371"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1813122.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            19223..19951
                     /locus_tag="ACL8LA_001372"
     CDS             19223..19951
                     /locus_tag="ACL8LA_001372"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813123.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            19993..20862
                     /locus_tag="ACL8LA_001373"
     CDS             19993..20862
                     /locus_tag="ACL8LA_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGS1813124.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            20837..21277
                     /locus_tag="ACL8LA_001374"
     CDS             20837..21277
                     /locus_tag="ACL8LA_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGS1813125.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            21400..23946
                     /locus_tag="ACL8LA_001375"
     CDS             21400..23946
                     /locus_tag="ACL8LA_001375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGS1813126.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
BASE COUNT         7577 a         4062 c         5165 g         7160 t
ORIGIN      
        1 aggtgtacta ttctagtttc aatatactat aaaagattga acgtttaggc aattccaaga
       61 ctacttaatc gaacattaac agcagtctcg gactgatgaa ggtctcttta tgaagttagt
      121 ggaggaagtc ggaaaagtag ccgaggtttt gaataggcgc tctggtcgaa aagaagggat
      181 aggattcgaa cgaggaactt gccagagaac tggctgatat catttactac accgtcgcaa
      241 tcgtagctat taatcatatt gaccttacca agactgtctt tgagaaagac aaaaccgcag
      301 ccgttaatta ccaacataaa cataatttgg aaggattatt gaagggaaag tagagatgtt
      361 gtccccttcc tccaacttga gaaaatcctt ggaacgtgat aaaataaagg ataatgattt
      421 ttaataattc attatataga atgagtggat ttctttatga agaggtttga agtatctaca
      481 gaaattggta gcctctctgt tacttatcaa aagcaaaaga aagtgctagt ttgtctaaac
      541 ggagcaggtt tgctaccaag ttatgaaaat ttttcactta tacttgaaaa acctcctcct
      601 acaattggtt atttgacaat tgattttccg aacacaggta ggagtccgat tcatgaccaa
      661 gctggaaaaa atctagataa tcttgcagat gcggtttatg aagtacttga agaattagga
      721 atttctgaat atatactttg tgcacatagt tggagtggaa ttttagcttg caaattactc
      781 gagaaaccaa ttaagcgtca gactttagta gcaattgaac cgacaactaa aaaagtcatg
      841 tttgctgatt tttcagaaaa tccttatcca aaaatggaag agcagatgag gctgattgac
      901 gagtgtggtc ctgaacttta ttttaagaac ttaactcaag caacatttag tcctgaaacg
      961 aataaaaaaa tctgggaatt aatgcaagaa aaaggcttag agttggaaaa tcaagatcca
     1021 gaatttcaga tatctggaga gattactgag gaagattttg agaatttgtc gatagaatct
     1081 catgtccctg tttttatttt ttgtcagact tatagagaaa aagagtacag agaatcagaa
     1141 tattggactt ccaatactaa actcatttta ggagggaatc accattattt acaatggtca
     1201 gaatcggaaa aaattgcggc tattattcga gaattgtcag aataagatgg aaaaaaggag
     1261 attacaggag acaagatgaa ctactttaat attgggaaaa tcgttaatac gcagggatta
     1321 cagggtgaga tgcgagtctt gtctgtgacg gattttgcag aagaacggtt taaaaaagga
     1381 gctgagctgg ctttgtttga tgaaaaagat cagtttgtcc aaacagtgac catcgctagc
     1441 caccgtaaac agaagaactt tgacattatt aaattcaaag atatgtacca tatcaatact
     1501 atcgaaaagt acaagggata cagtctcaag gtcgctgagg aagatttgaa tgacctagac
     1561 gatggtgaat tttactatca cgagattatc ggtttggaag tctatgaggg tgatagcttg
     1621 gttggaacca tcaaggaaat cctgcaacca ggtgctaatg atgtctgggt ggtcaaacga
     1681 aaaggcaaac gtgatttgct tttaccttat atcccaccag tggttctcaa tgttgatatt
     1741 ccaaataaac gggtcgatgt ggaaatctta gaagggttag acgatgaaga ttgatatttt
     1801 aaccctcttt ccagagatgt tttctccact ggagcactca atcgttggaa aggctcgaga
     1861 aaaagggctc ttggatatcc agtatcataa ttttcgagaa aatgctgaaa aggcccgtca
     1921 tgtagatgat gatccctacg gaggcggtca gggcatgttg ctcagagcac aacctatttt
     1981 cgattccttt gatgctattg aaaagaaaaa tccgcgcgtt attctccttg atcctgctgg
     2041 aaagcagttt gatcaggctt atgctgaaga tttggctcaa gaggaagagc taatctttat
     2101 ctgtgggcac tatgagggtt atgatgagcg cattaagacc ttggtaacag atgagatttc
     2161 cctaggcgac tatgtcctca ctggtggaga attggcagct atgaccatga ttgatgctac
     2221 agttcgcctg attccagaag tgattggcaa ggagtctagc caccaagatg atagtttttc
     2281 ttcaggtctt ttagaatatc ctcagtacac acgtccctat gattatcgag gcatggtcgt
     2341 gccagatgta ttgatgagtg gccaccatga aaagattcgt cagtggcgat tgtacgagag
     2401 tttaaagaaa acctacgagc gcagaccgga tttacttgaa cattatcaac tgacagtaga
     2461 agaagaaaaa atgctggcag aaatcaaaga aaacaaagaa taaaggagaa acctatgcaa
     2521 gtaatcaaac gtaatggcga aattgctgaa tttaatccag ataagattta ccaagccatc
     2581 ttgaaggcag cccaaactgt ttatgttttg acagatgatt tgcgtcaaaa tcttgctcaa
     2641 gtcactaaga aggtagtttt ggatttacaa gaagccaagg tggaacgtgc gactatcagt
     2701 atgattcaat ctatggttga acatcgttta ttgggcgcag gttacattac cattgcagaa
     2761 cactacattt cctatcgttt acaacgtgac ttggaaagaa gtggttatgg agatcatatc
     2821 gcagttcatt tacattttga acaaattcgc taagaaaaaa gagtgggatg aagcaactac
     2881 atctcactct ttcttaaatc tatttttttg ggctgtttgg acagttgagg ggacaaatcg
     2941 caggcgttga atatcgctta tacggataat ccggctcaca tttttggcaa aatttttcac
     3001 gataatttgc tggccttgct gatcatattt gatgatatca cctgtaaagc ttgtctcaga
     3061 caagataagg tgaacagcgg tttgtttctg gatagcctct tcaatagtgg ctgtaaggga
     3121 gttggtttca gtctggtcag gttggtcatg tccatttaaa aaatcatgta ttttatctag
     3181 aacttgctgg aatttatgtc tcatattggc ctcccttctt ctatatcaat attatataaa
     3241 attttcctaa aatctacaag attttacgaa taaactaatg aaactaaaaa aggagaagaa
     3301 aatggcagaa tttacatttg aaatcgaaga gcacttgttg actctttctg aaaacgaaaa
     3361 aggttggacc aaggaaatta accgtgtgag ctttaatggt gcccctgcaa agtttgatat
     3421 tcgtgcttgg agtccagacc atactaaaat gggcaaaggg attactctct caaatgaaga
     3481 atttcaaacg atggtggatg cctttaaagg caactaatac tcttcgaaaa tctcttcaaa
     3541 ccgcatcagc tttgccttgc cgtatgtatg gttactgact tcgtcagttt catccacaac
     3601 ctcaaaacag tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat
     3661 tgagtataag ttttcaaaat aaaaaaaaag gatattgagt catgataact cgatatcctt
     3721 ttttagttag taaagtaagc ctgattttta aggcgttgaa gtaggggacg gctaataaaa
     3781 ctaatagcca gaagtttgcc aaggtctgga atgataaagg gaagaacccc cacaacaaga
     3841 gctttttcaa atgccattcc agctaggaaa tgcaagctga gaatcccgcc gacaaagaca
     3901 agggcatcac ccaagaggtt tgcaagaaaa atcttaacaa caccactctt gctgttggtt
     3961 agagaggaag taagtccaga gtaaacgaga taaaaccaaa gatagcctgc agtagggcca
     4021 actaaagcct gaaaaccagc tccacctcct gcaaagacag gaagaccgat agcacctaga
     4081 agaagataga gtccagcaga aagtacagcc tctctcggtc taaagacagt agcaatcaag
     4141 ccgattgcaa agttttgcag agtgaaggga acaggtccaa ttggaagact gatttgtgcc
     4201 aaaacagcaa tgagagcagc cccaatagca gggatagcat aaacgtgagc ttttttcaaa
     4261 atagttaacc tcttttctaa tgtatagtaa ctattatact catataggag tcgatgtcaa
     4321 ccatttttta aaaacaaggt aacaaaaact gtaacagccg attttcgtga ctagtcaagt
     4381 tggataattt ttttgttgat tcgaaaattg tctgtataga taatcctatt ttattaataa
     4441 aaaatatact agtaaatact tgacaaatta aaaaataagt gttacattaa aaatgtaaac
     4501 ggatacattt agatgtgttc aattttaaaa ggaggggtag ttatgagata ttttaacgac
     4561 tctagatacc ataacggtga ttaaaaacct aaatataatc aattcatatt aaagtaatat
     4621 ggagggacta aaaagagact attatttaat cttcatcaaa tatttaaatt caaggagaag
     4681 taaaatgaaa aaaagaatta ataagaaagc atctgttttt ctagttgcaa gtgttttcag
     4741 ttttagcttt gctactacag ctagtgtcgt taacgcagta acggcacacc ctgctccaag
     4801 tcccattatt gatgtatggg aatatggtgt aaatgataac tatggttatt cgaattactt
     4861 tgttagttct ccaagtaata ttggatcaaa atcttcagta acgaattttt ggggaacagt
     4921 taaagctcag gataaaaaag attatggatg ggcatattca agtgcaacaa aatcttggaa
     4981 cgatgttcgc ttaaatgcat attgggatta ttatagattt taaaaataat ttatgaaaac
     5041 aagagaccca attataagat tgggtctctt gtaatatggt aattgcttat gaaattaaaa
     5101 ttatgtatca ttggattctt cttttgtttg attgctacaa ttggtctggt cactattagt
     5161 gataccgaaa tccccatacc tttaccaatt gatggagctt tttctatcca aggaaaaagt
     5221 aatctttcta ataacgaaat atacgaaatg gttcgtgact tatcaaaaac ggaaaaggtt
     5281 accatttaca agccaatcgt tcagagttcg ggtcagttga agtatgtaaa ttttgatgat
     5341 gtaaataatg aacaattaaa gtcagcacca atagtaggga tgtattacac acttgggaaa
     5401 atggatgtag acagtttgaa accacttacg atgacgggac tacaaacagt ctatatggct
     5461 tatccttggt atataggagg aatattacaa ttcactggaa ctttaagaat actgttaatg
     5521 ggttcaattt atttaacttt attagttgtt ttatttgttg ttaggacgag gcagatcaaa
     5581 gaaggagtga tacgacgttc tttgggcttg cctatatacg acttaagaag agagtatggc
     5641 atttctctta tttttgaact gattatgatg gctttattga tgatttctta cagttctttt
     5701 ttgggtaatg gtttcttcac ctatagttct aagttatttt tctctctgct tttaacgaat
     5761 tttatactat ttcaaatcat tgatcttatt acttttgttt tattttggtt gacgattcag
     5821 attgaaaagc ctattgaaat tattaaaaac aaggcaaaaa acaagcttat ttttgtcgta
     5881 tggcttgcta ttatttcgat tatcatcctt gtctcaggag tttttttaca agaaacaaag
     5941 agtagtcaat ctagtattaa catacagata cagaatttag taccatggga tacagtaaaa
     6001 gactggagaa ggattgagtt ccttggaatt gaaagtaact ctactaaaaa tagagaagtt
     6061 aatgattcag atggtcagta tctacagata gttgctgcct taaaaaactt agatttctta
     6121 tatatagaac ggtcttcagc atatgttcca gatttcatga aaacttcaca tgttatagaa
     6181 aatttttcta aacaattaga aaatgacggg ataacgaatc cagaaataaa taaagagtta
     6241 atttatatca accaaacagg tgctaaccta caaaataaag tgaatggaac aaactatcat
     6301 cttttagata ataagatagc aaccatctat attcctgaga agtggaaaga aaaccagaaa
     6361 tctattgaga atacagttgt agcagaacaa tttattggaa caaactatac aagagaacag
     6421 cttgcagttc aaataattcc tgatggggaa aaaatttttt attttaatga ggatgctgat
     6481 aataatctta aaatgaaaga tattttaccg ctggcaaatg tagcagatag caaagataat
     6541 atagttgttg tgttagatac ggacaaaatg atggaaaata ataagttttc tttggctagt
     6601 aacatactat ataaatctct ttttagtcct gaagcggtaa aaaaaataaa tgagatgact
     6661 gtcctgttga atttctcaat gaatccagta gatgtttacc aaattgtgaa attaaagatt
     6721 caatccttag agcaccaaat tttactttca caaatcttgc agaagataat ttacagtatt
     6781 gtctttatac tcatttatca atatgtccaa ctttttatta ctctaaaaca gaatgaatat
     6841 gtgaaaaaaa tcattttggg tctgtcaaaa acatatatag caatttcaag tctcaaatat
     6901 tttatgatga ctattacaat ggttattcta tttacatttc ttatgacagg gcaaatagag
     6961 ttgctatata ttggagcggc ttctctgcta gttttgatgt tgtcaatcat aatgagtttt
     7021 agaaaattat ctgaaagcta cactaaaatt ttaaaaggag atgaatcatg acaattgacc
     7081 ttttgaacgt ttcaaaaagt ttcggctcaa agaagatctt tacagactta aatttaatat
     7141 ttgaatctgg aaaaagctat gcattgattg gaggttctgg ctccggtaaa agtacccttc
     7201 ttaatattat aggaagatta gaaaaaattg acagtgggaa tgttttagtt gataaacaag
     7261 atatttggaa gataaaagaa agaacttttt ttaaaaatac tgttggatat gtatttcaga
     7321 attattcttt aatagataac aaaacagtgt atgataatct gagccttatc actaaagaca
     7381 aaaaaacaat aactgacgta cttgaaaaag taggactgtc aagtgactat ttacaccaaa
     7441 aaatatatga attgtctgga gggcaggctc aacgtgtagc tattgccaga atgttaatga
     7501 aaccacgtaa aattatctta gcagatgaac ctacaggagc tttagatggt gagattggaa
     7561 aagaaattat tcgtttacta ttgaatgaaa cagctgaaga taaatatgtc attatagcga
     7621 cacatgatcc agctgtctat aacgaagttg atgtgataat tgacatgaaa gatatagggt
     7681 ataaggtatg aaaaagaaaa tttatattgc tttgatcttt gtaactgggg tactcgccat
     7741 tttctttttt ggaaaacaaa tgattacaaa agaaaatatc aataagccaa cggtagagtt
     7801 aacaatctat acgctttctt ctagtgatac agaaaaatgg aataaagtga gacaagtaga
     7861 aacggaagaa gctatatact tcataactgt gaaagaagta tcatcttcag aagaagtatt
     7921 ttcaaacatc attgcaaatg gagctgctac ggggttcgga gttcgtgaag aagaagtgaa
     7981 gaaattcaat aatggtttag gagacaccat agaggactct aagcataata aactaattga
     8041 aatagaattt tttacttttt ctgatgatgg cgcgggattt gtggtagcaa actttgacta
     8101 cggtaaagaa gagttgaatt ctcagaaaaa agatataaaa gaattatata aaaaaatata
     8161 tgaatctttt aaagaaaaga ataaatgatt gtattataat ccttgtattt tgctatggtt
     8221 tgttaattga tttttgcata tcttacttca acatactata aaataaaaca taagaaaacg
     8281 agtatctcca actacggaaa tactcgcttt ttgatattta gaagtgtttt ttgctttgct
     8341 tcttttgttt cgattaacgc atggttacaa attcttcaga tgcagttggg tgaattgcca
     8401 cagttgcatc aaagtcagcc ttggttgctc ccattttgat agcaacggca aatccttgaa
     8461 tcatttcatc aacgccgtag ccaattccat gaagtccgac aactttttct tctgaaccag
     8521 ctgttatgag tttgaaacgg gattcttgac ggttgcgagt gcaagcagag tacatagatg
     8581 caaagcttga tttataaacc ttgatttggt cttgaccgta ttctttaata gcttgctctt
     8641 ctgtcaaacc aacagttccg atagcagggt gtgaaaagac aacagttgga atagttgagt
     8701 aatccatttt tgcagtagtt tttccgttaa agagacgttc agatagggta cgtccggcct
     8761 tgattgcaac tggagtcagt tctttctcgc ccgttacatc acctagagca tagattccct
     8821 caacaacagt attttggtat tcatccactt ggataaagcc acgttcgttc agagtcactc
     8881 cagctttttc aagttgcaag cccttaacgt ttggacggcg acctgtagcc cagataactt
     8941 ggctagctgt gtgactagta ccatcttcga aatgaatggt aatgccgtca gtagtttttt
     9001 ctaacttgac agggactttg tgagtgtgaa gtggtaagtt tgttctttcc atttccttga
     9061 ccaaaccttc aacgatgtag gaatcaaaac cacgtaaagg acgatcgcgg cgaacaaaga
     9121 gatctgtctt gacaccaaaa gtgtggagta cgccagctaa ttcaacggcg atataaccag
     9181 cgcctagaat ggcaattgac tctggaagtt cttcccaggc aaatacatca tcagaagagc
     9241 cacctagctc agcaccagga atattaggaa tacttggatg ggcacctgta gcaatcacga
     9301 tatgtttagc acgaatcagt tcaccattta cgcttacagt atgagaatct acaaattcag
     9361 catgaccttc aatcaagtct acaccgttgc gtttaaaact accatcataa gaagaacgag
     9421 cgcgatcaat gtaggcttca cgattgcgac gtagggttgc aaagtcaaag ttaagatcag
     9481 tagtcttaaa gccgtagtct tctccaaatt gatggaaagt ctcagcgatt tgcgccccgt
     9541 accacatgat ttttttagga acacaaccga cgttgacaca ggttccacct aatttctttt
     9601 cctcaataac ggctgctttg gctccatgtt caccagcacg gttcatggta gcgattcctc
     9661 cgctacctcc accgatagca atgatatcat attctctcat tgaaaactcc tttgtaatct
     9721 tttaaatagt agagcgtctt ttttgatagt tatattttat tctttttttg atttgattgc
     9781 aaaaatctgc tacgcacaaa aaattgcaaa aaagcttgcc aaataagtca agatagtgta
     9841 ttatatatct agtacaatag aaaagcaaaa ttccgaacga ttgtttaatc ctgaactgta
     9901 ttgtttgctg gagggaaatt tggtataata gcaaagtatt gtaaaaaaga aaaggagttt
     9961 cattatgaag aaaaagaatg gtaaagctaa aaagtggcaa ctgtatgcag caatcggtgc
    10021 tgcgagtgta gttgtattgg gtgctggggg gattttactc tttagacaac cttctcagac
    10081 tgctctaaaa gatgagccta ctcatcttgt tgttgccaag gaaggaagcg tggcctcctc
    10141 tgttttattg tcagggacag taacagcaaa aaatgaacaa tatgtttatt ttgatgctag
    10201 taagggtgat ttagatgaaa tccttgtttc tgtgggcgat aaggtcagcg aagggcaggc
    10261 tttagtcaag tacagtagtt cagaagcgca ggcggcctat gattcagcta gtcgagcagt
    10321 agctaaggca gatcgtcata tcaatgaact caatcaagca cgaaatgaag ccgcttcagc
    10381 tccggctcca cagttaccag cgccagtagg aggagaagat gcaacggtgc aaagcccaac
    10441 tccagtggct ggaaattctg ttgcttctat tgacgctcaa ttgggtgatg cccgtgatgc
    10501 gcgtgcagat gctgcagcgc aattaagcaa ggctcaaagt caattggatg caacaactgt
    10561 tctcagtacc ctagagggaa ctgtagtcga agtcaatagc aatgtttcta aatctccaac
    10621 aggggcgagc caagttatgg ttcatattgt cagcaatgaa aatttacaag tcaagggaga
    10681 attgtctgag tacaatctag ccaacctttc tgtaggtcaa gaagtaagct ttacttctaa
    10741 agtgtatcct gataaaaaat ggactgggaa attaagctat atttctgact atcctaaaaa
    10801 caatggtgaa gcagctagtc cagcagccgg gaataataca ggttctaaat acccttatac
    10861 tattgatgtg acaggcgagg ttggtgattt gaaacagggt ttttctgtca acattgaggt
    10921 taaaagcaaa actaaggcta ttcttgttcc tgttagcagt ctagtaatgg atgatagtaa
    10981 aaattatgtc tggattgtgg atgaacaaca aaaggctaaa aaagttgagg tttcattggg
    11041 aaatgctgac gcagaaaatc aagaaatcac ttctggttta acgaatggtg ctaaggtcat
    11101 cagtaatcca acatcttcct tggaagaagg aaaagaggtg aaggctgatg aagcaactaa
    11161 ttagtctaaa aaatatcttc agaagctacc gtaatggtga ccaagaactg caggttctca
    11221 aaaatatcaa tctagaagtg aatgagggtg aatttgtagc catcatggga ccatctgggt
    11281 ctggtaagtc cactctgatg aatacgattg gcatgttgga tacaccaacc agtggagaat
    11341 attatcttga aggtcaagaa gtggctgggc ttggtgaaaa acaactggct aaggtccgta
    11401 accaacaaat cggttttgtc tttcagcagt tctttcttct atcgaagctc aatgctctgc
    11461 aaaatgtaga attgcccttg atttacgcag gagtttcgtc ttcaaaacgt cgcaagttgg
    11521 ctgaggaata tttagacaag gttgaattga cagaacgtag tcaccattta ccttcagaat
    11581 tatctggtgg tcaaaagcaa cgtgtagcca ttgcgcgtgc cttggtaaac aatccttcta
    11641 ttatcctagc ggatgaaccg acaggagcct tggataccaa aacaggtaac caaattatgc
    11701 aattattggt tgatttgaat aaagaaggaa aaaccattat catggtaacg catgagcctg
    11761 agattgctgc ctatgctaaa cgtcagattg tcattcggga tggggtcatt tcgtctgaca
    11821 gtgctcagtt aggaaaggag gaaaactaag atgcagaatc tgaaatttgc cttttcatct
    11881 atcatggctc acaagatgcg ttctttgctt actatgattg ggattattat cggtgtttca
    11941 tcagttgttg tgattatggc tttgggtgat tccctatctc gtcaagtcaa taaagatatg
    12001 actaaatctc agaaaaatat tagcgtcttt ttctctccta aaaaaagtaa agatggttct
    12061 tttactcaga aacaatcatc ttttacggtt tctggaaagg aagaggaaat tcctgttgaa
    12121 ccgccaaaac cgcaagaatc ctgggtccaa gaggcagcta aactgaaggg agtggatagt
    12181 tactatgtaa ccaattcaac gaatgccatc ttgacctatc aagataaaaa ggttgagaat
    12241 gctaatttga caggtggaaa cagaacttac atggacgctg ttaagaatga aattattgca
    12301 ggtcgtagtc tgagagagca agatttcaaa gagtttgcaa gtgttatttt gctagatgag
    12361 gaattgtcca ttagtttatt tgaatctcct caagaggcta ttaacaaggt tgtagaagtc
    12421 aatggattta gttaccgggt cattggggtt tatactagtc cggaggctaa aaggtcaaaa
    12481 atatatgggt ttggtggctt gcctattact accaatatct cccttgctgc gaattttaat
    12541 atagatgaaa tagctagtat tgtctttcga gtgaatgata ccagtttaac cccaactctg
    12601 ggtccagaac tggcacgaaa aatgacagag cttgcaggct tacaacaggg agaataccag
    12661 gtggcagatg agtccgttgt atttgcagaa attcaacaat cgtttagttt tatgacgacg
    12721 attattagtt ccatcgcagg gatttctctc tttgttggag gaactggtgt catgaacatc
    12781 atgctggttt cggtgacaga gcgcactcgt gagattggtc ttcgtaaggc tttgggcgca
    12841 acacgtgcca atattttaat tcagtttttg attgaatcca tgattttgac cttgttaggt
    12901 ggcttaattg gcttgacaat tgcaagtggt ttaactgcct tagcaggttt gttactgcaa
    12961 ggtttaatag aaggtataga agttggagta tcaatcccag ttgccctatt tagtcttgca
    13021 gtttcggcta gtgttggtat gatttttgga gtcttgccag ccaacaaggc atcgaaactt
    13081 gatccaattg aagcccttcg ttatgaatga gatcaacaag atggacactc gtctatcttg
    13141 tttttgtatg gatttcccta tcgaaattca ttaaaaatgt gatataatga agtgttagaa
    13201 aaacaggttt catttgcctg gaaaggaaaa attatgtctg aaaaaaattt ttatattaca
    13261 acgccgattt actatccatc tgggaaactt catatcggtt ctgcctacac aactatcgca
    13321 tgtgatgtcc tagcacgtta caaacgcctg atgggctacg atgtatttta tctgacaggt
    13381 cttgatgaac atggtcagaa aatccagcag aaagcggaag aagctggtat tacacctcaa
    13441 gcctatgttg atggaatggc ggttggagtt aaagaactct ggcaattact agatatctca
    13501 tacgataaat ttatccgtac aactgatgac taccatgaaa aagttgtcgc acaagtcttt
    13561 gaacgcttac ttgctcaaga tgatatctac ttgggtgaat actctggttg gtattcagta
    13621 tcagacgaag aattctttac agaaagccag ctggcagaag ttttccgtga tgaagctgga
    13681 aatgtgactg gtggtattgc tccatcaggt catgaggttg aatgggtttc tgaagaatca
    13741 tacttccttc gccttagcaa ataccaagac cgtttggtcg aatttttcaa agctcatcct
    13801 gaatttatca cgccagatgg tcgccttaat gaaatgctac gcaacttcat cgagccaggt
    13861 ttggaagatt tggcggtatc tcgtacaacc tttacatggg gagtgcctgt cccatcaaat
    13921 ccaaaacacg ttgtctacgt ttggattgat gcccttctta actatgcgac agctcttggc
    13981 tacgctcaag acgaacatgg taactttgac aagttctgga atggaacagt cttccatatg
    14041 gtaggaaaag atatccttcg cttccactct atctactggc caatccttct tatgatgttg
    14101 gatgttaaat tacctgatcg tttgattgcc catggttggt ttgtcatgaa agacggaaaa
    14161 atgtctaagt caaaagggaa tgtcgtttac cctgaaatgt tggtagagcg ttatggacta
    14221 gatccacttc gttactacct catgcgtaac cttccagttg gttcagacgg aacctttact
    14281 cctgaagact atgtcggccg tatcaactat gaattggcta atgaccttgg gaacctcctt
    14341 aaccgtacgg tttccatgat taataagtac tttgatggac aaatccctgc ctatgtagaa
    14401 ggtgtgactg aatttgatca tgttcttgct gaggttgcag aacaatcaat cgcagacttc
    14461 catacacaca tggaagcagt tgactatcca cgtgcgcttg aagcagtctg gactctgatc
    14521 tctcgtacca ataaatacat cgatgagact gcaccatggg tcttggccaa ggatgaagct
    14581 cttcgtgacc aattggcaag tgtcatgagc cacttggcag ccagcattcg tgtagttgct
    14641 cacttgattg aaccatttat gatggaaact agtcgtgcag ttttgactca acttggtttg
    14701 gaagaagttt ctagtcttga aaacttgagt ttggctgact tcccagcaga tgtgactgta
    14761 gttgccaaag gaacacctat ctttccacgt ctaaatatgg aagaagaaat cgcctatatc
    14821 aaggaacaaa tggaaggcaa taaaccagca gtcgaaaaag aatggaatcc ggacgaagtt
    14881 gagctcaaac taaacaagga tgaaatcaag tttgaagact ttgacaaggt tgaaatccgt
    14941 gtcgcagaag tcaaagaagt gtctaaagta gaaggttcag ataagttgct tcaattccgc
    15001 ttggatgctg gtgatggaga agatcgtcag attctttcag gaattgcaaa atactatcca
    15061 aatgaacaag aattggtcgg caagaaagtt caaatcattg ctaacctcaa accacgtaaa
    15121 atgatgaaaa aatatgtcag ccagggtatg attctctcag ctgaacatga tggcaaatta
    15181 acccttctca cagttgatcc agctgtacca aatggaagtg tgattgggta aaagtagaca
    15241 ggactagttc aggctagttc tgttttttta tttatactca atgaaaatca aagagcaaac
    15301 taggaagcta gccgcaggtt gctcaaaaca cagttttgag gttgtagatg aaactgacga
    15361 agtcagctca aaacacagtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
    15421 ataaggtagg gcgacgctga cgtggtttga agagattttc gaagagtatt aactattatg
    15481 ctttacaaac tagtgtagaa gtgatatact ataggtgaag ttactagtag gtattacaaa
    15541 atagattaga agggaaaagt gatgaaagaa actcagctat taaaaggtgt tcttgaaggt
    15601 tgtgtcttgg atatgattgg tcaaaaagag cggtatggtt atgagttggt tcagactttg
    15661 cgagaggctg gatttgatac tatcgttcca ggaactattt atcctttgtt gcaaaagtta
    15721 gaaaaaaatc aatggataag aggcgacatg cgcccgtcgc cagatggtcc agatcggaag
    15781 tatttttcat taatgaaaga aggagaagag cgtgtctcag tcttttggca acaatgggac
    15841 gatttgagtc aaaaagtaga agggattaag aatgggggtt aaaccatgaa gaaaatgaag
    15901 tattacgaag aaacaagcgc tttgctacat gagttttctg aggagaatca aaagtatttt
    15961 gaggagttgt gggaaagttt taatcttgct ggatttctct atgatgaaga ctatctcaga
    16021 gagcagatct atttgatgat gctagatttc tcagaagcag aacgagatgg catgagtgca
    16081 gaggattatc taggtaagaa tcctaaaaaa ataatgaaag agattctcaa gggagcacct
    16141 cgcagttcta tcaaagagtc ccttttgacg ccaattcttg tcctggcggt attacgttat
    16201 tatcaactac taagtgattt ttctaaaggt cctctattaa cagtcaattt gctcacattt
    16261 ttagggcaac ttcttatttt tctgattgga tttggacttg tggccacaat tttacgaaga
    16321 agtttagtcc aagattctcc taaaatgaaa attggcactt acattgttgt tgggactata
    16381 gttcttctag ttgttttagg atatgtagga atggcaagct tcatacaaga aggagtcttt
    16441 tatattccgg ctccctggga tagtttgtct gtctttacga tttcgctagt tatcggtatt
    16501 tggaattgga aagaagcggt ctttcgtcca tttgtcagta tgattattgc ccatcttgtg
    16561 gtgggttctc tgctccgtta ttatgagtgg atgggaattt caaatgtttt ccttacaaaa
    16621 gttattcctt tagctgtcct ctttattgga atctttgtct tgttccgtgg gtttaagaag
    16681 ataaaatgga gtgaagtata gtcaaaaagc cgttgcaaag cggttttttg ttataatgaa
    16741 gggaagaatg ttagaaatta aaggagaaaa atttgatgag atacataact cttggtcaag
    16801 atgacaaaga attatcagaa attgttctcg gaatgatgag aataaaagat aaatctgtaa
    16861 aagaagttga agagcttgta gaaacagcac tttctgttgg aatcaatgcc ttcgacttgg
    16921 ctgatattta tggtcgtggt cgttgtgaag aactgttagg tcttgtccta aaaaatcgtc
    16981 cagatttaag agaaaagatg tggattcagt ccaaatgtgg cattcgcatt gaagaattta
    17041 cctattttga tttttctaag gactatatta taaaatcagt agacggtatt ttgcaaagat
    17101 tgaagattga tcatctagat agcttgctcc ttcatcgacc agatgctttg atggaatctg
    17161 accaagtagc agaagccttt gatctccttt ataaacaagg taaagttcga gattttggag
    17221 tttctaatca aaatcctatg atgatggagt tgcttaaaaa agatgtcaag cagccgttag
    17281 ctgttaatca gctacaattg agtgcggctt ttactccagg atttgaatca gcttttcatg
    17341 ttaatatgga agatagtcaa gcagctatgc gagatggcag catttttgaa tattgccaat
    17401 tacacgatgt ggtcattcaa gcatggtctg tcttgcaatt cgggtatttt aaagggaact
    17461 ttgttggaaa tgagaaattt caagctttaa atcaagtact tgatcgttta gctattaaat
    17521 atggagtaac ctcttcaact attgccattt cttggatatt gcgttatcca gcaaaaatgc
    17581 aggcagtcgt aggtacaaca aatcctaagc acttgagaga agttagccga gcggcaaact
    17641 ttagcttaac aagaaaagaa tggtatgaaa tttatcttgc tgcagggaat aatttgccgt
    17701 aagtagtagc aggtttgaat aactcacagt caaaaagccg tataaagtgg ttttttgcta
    17761 taattaaatg aagaatgtag aattaaagga gaaaaacatg acatttgaag agatcctgcc
    17821 tgggttaaag gctaagagaa aatatgtacg aactggttgg ggaggtgcgg aaaactatgt
    17881 ccaactcttt gataccatcg agcaaaatgg gcttgcactt gaaatgacac cttatttcct
    17941 aatcaacgtt tctggagaag gagaaggttt ttccatgtgg agcccgacag tttgtgatgt
    18001 tttggcaacg gattgggtag aagtgcatga ctaaacgtgt actcattaca ggagtgagtt
    18061 cagggatcgg attggctcaa gctcgcctct ttttagagaa gggctatcaa gtttatggag
    18121 ttgaccaagg tgaaaagcca ctcttagagg gtgattttca ctttttacag agagatttga
    18181 ccttggactt ggaacctatt tttgactggt gccctcaggt ggatgttttg tgtaatactg
    18241 ccggagtttt ggatgcttac aaaccactgt tggaacaaac ggcgcaggac attcaagaga
    18301 tttttgaaat caactacatt attccagtag agttgactcg gtattatttg acacaaatgc
    18361 tggaaaataa aaagggaatc atcatcaata tgtgttccat tgcttctagc ctagcaggtg
    18421 gaggtggtca cgcctatact tcatccaagc atgccttggc tggcttcacc aagcagttgg
    18481 ctctagacta tgctgaagct aggattcagg tctttggtat cgctccagga gcagtcaaga
    18541 cagctatgac cgctgcggat tttgagacag gtggcttagc tgactgggtg gctagtgaaa
    18601 ccccaatcaa gcgctggatt gaaccagagg aagtggcaga agtcagtctc tttttggcca
    18661 gtggaaaggc atctgccatg cagggacaaa ttctgacaat agatggtggc tggtctttga
    18721 agtaggagga gttggatgga aatcaaaaat cactttgggg tctactgtgt ttgctttgaa
    18781 aatggaaagt tactttgcat tgaaaaaacg agaggccctt atcaacatcg gtatgatcta
    18841 cctggaggca gtcagcaact tggtgaagga ctgacggaaa cgctgactag agaagttatg
    18901 gaagagacgg gatttactgt tagaagctac tctaatcctc gaatctacga tgttttcgtc
    18961 agggaagagt taaaaaattt tatggttcac catgtcatag ccttgtatga tgttgaaatg
    19021 aatgagagtg cacctcaagt tacgatttcg gaagctgtgt ctgatggtgc gaatgattca
    19081 cttggatata tttggatgga tattcaagag attacagaag aaaatgcatc gccattagtc
    19141 ttgaaggtta agtctgaatt attaggattg ccagaactgg acaagacttc ttacatgaat
    19201 tggaaggtga agtagaggga taatggaact atttaaaaca tggaagaaaa atatggttct
    19261 ctatggtctt aaatctcaaa ttggaactgt ctaccgaaac aatgatagga caacaagctt
    19321 ttatgatgtt gggaattttc tatacctagc aggggagttg gattccagat tttgggaaga
    19381 ttttgttaga aaatatggtt tagattataa gattattatt tcagaaaata ctaattggca
    19441 agattttctg catcgaaaag tggggctaaa ttcttttact cgctattctt ttaaagataa
    19501 ggcaaatttt caagttgaat ttttaaataa tctagttact catttagagg aaggttacaa
    19561 tattgtgcct attgataatc atatttataa ctgcttttct acggaagaat ggtcacagga
    19621 tttacagggg gattttgagt cctatcagga ttttgtttta aaaggtggat ttggctttgt
    19681 gattcttaaa aataatgaac tgattgctgg gatttcctca gggttagttt accgtaaagc
    19741 agttgaagtg gaagttgcaa ctagaccaaa cgaacaagga aatggatttg ctaaaaagct
    19801 tggtgctgca atgattctag agagtttaaa tagagatatg tttccacttt gggatgctca
    19861 taatgaggct tccaaaaaag tagcagaatt tttaggatat gagttatctg aaccttatga
    19921 agcttttgaa ctagaggaaa ttttgatata attatatatg atattgtatc ttttaagacg
    19981 aggtgaatgg ctatgagaaa tcaacttgcc cttagtggcg aaaaaattct tgaaaaaatt
    20041 tatcctcaac tatttcacca cgtcggcatg attcgtgggg aatatttgtt gagagagctt
    20101 aatcaaaaca tcatattagc aagttgtcag caatttgtaa aagattatct agagactatc
    20161 tgctccctgt actcagatga agaagtttgg tatcgttttt ctgaattaac gaatacagaa
    20221 gccaattgtt tagtggggac tagagagttt tttgacgaag ggcatccctt gtttggctat
    20281 agaggaacca ggcgtttact ggcgtgtttg gatgaatttc aggctgaagc acatgtcgtt
    20341 acagaagttt atcaaaccaa tcccaatcta tctcttatct ttccttttgt caatgatgcc
    20401 gaccaattaa aacaagctat tacagtattg cgtcagcagg gttttactgg gaaagtcgga
    20461 acgatgattg aattaccgtc agcgtatttt gacttatcta gtatactgga aacgggcatt
    20521 tcaaagattg tagttggaat gaatgatttg acttcctttg tttttgcgac tatgagaaac
    20581 agtcaatggc atgatatgga aagtccaata atgttagata tgctaagaga tatgcaggat
    20641 aaagcaagaa agaacaagat tgattttgct gtagcaggct atctgaatac ttctttcata
    20701 caaaaaatga atcagttggg tatcaagtgc attatccact acagttctat tccagagatt
    20761 tttgatttag agattgacca tccagatcat cttaaacaca taaaagaaga aagtaaaaaa
    20821 ttacaaagga gtacccatga taccgcaaga aatgtggaat aaatacaagc aaatcaatcc
    20881 tttgatcgga gatgaaatcg atgcttgggc ttttggagtt gaaccagatc ttttagcgga
    20941 tttggtgttt aaaggagaaa agacagcaac agcatcagcc tatgacctct atgtactaga
    21001 agacgaacca cttccccaag tagggacctt cgatatcatt ttagatagtc aaaatcagtc
    21061 tgtctgcatt gtcgaaatta caaaggtttc tgttgaactc ttcaatcaag tttctgcgca
    21121 acatgccttt aaggaaggtg agggagacaa atcacttgcc tattggcgcc aggttcatga
    21181 ggactttttc acagactgtt tgggtgaagt agggctgact tttacacctg aaagcaaggt
    21241 tgttttagaa gaatttcgca aggtctaccc actgtagact attagaagga agaaagtttt
    21301 ggaaatcgct gtccaatcct tttttttcaa gcaaaatatg atataataag tttgtttgaa
    21361 gaagagcagc agctcttaaa cttagaatag gagaaaacta tgcaagcagt tgaacatttt
    21421 attaagcaat ttgttcctga acattatgat ttatttttag atttgagtcg tgagaccaag
    21481 actttttctg ggaaagtgac catcactggt caagcacaga gtgaccgcat ctccctccac
    21541 caaaaagact tggaaatcac ctctgtagaa gttgcaggtc aagctcgtcc atttacagtt
    21601 gaccatgaca atgaagccct tcatatcgaa ttggctgagg ctggtcaagt tgaattggtt
    21661 cttgcctttt ctggtaaaat tacagacaac atgacaggga tttacccttc ttattataca
    21721 gttgatggag tcaagaagga ggtcttgtct actcagttcg agagccattt tgcgcgcgaa
    21781 gctttcccat gtgtggatga gcctgaagcc aaagcaactt ttgacctctc tcttcgcttt
    21841 gaccaagcag aaggtgaatt ggccttgtca aacatgccag aaatcgatgt tgaaaaccgt
    21901 aaggaaacag gtatctggaa gtttgagaca acacctcgca tgtcttctta cttgttggcc
    21961 tttgttgctg gtgatttgca aggggtgacc gctaaaacta aaaatggtac cctggtaggt
    22021 gtctactcaa ccaaagcaca tccactttca aatcttgatt tctcactgga tatcgctgtt
    22081 cgctctatcg agttttacga agattactat ggagttaagt atccaattcc tcaatctctc
    22141 cacatcgccc ttcctgactt ctcagctggt gctatggaaa actggggtct tgtgacctac
    22201 cgtgaagttt acttggttgt cgatgagaac tctacatttg ctagccgtca acaagttgcc
    22261 cttgttgtgg cccatgaatt ggctcaccaa tggtttggga acctcgtgac tatgaaatgg
    22321 tgggatgacc tttggctcaa tgaaagtttc gctaatatga tggaatacgt ctgtgtggat
    22381 accatcgaac caagctggaa tatctttgaa gatttccaaa caggtggagt acctcttgct
    22441 cttgaacgtg acgctactga tggcgttcag tctgtccacg tcgaagttaa acatccagat
    22501 gaaatcaata cactctttga cggcgctatc gtctatgcca aaggaagccg tctcatgcac
    22561 atgcttcgcc gttggctagg agatgctgat tttgctaaag gtttgcatgc ctactttgaa
    22621 aaacaccaat acagcaacac cattggtagt gacctttggg atgcccttgg tcaagcgtca
    22681 ggacgtgatg tcgcagcctt catggattct tggttggaac agcctggtta cccagttctt
    22741 actgtcaaag ttgaaaatga tgtcttgaag atttcacaaa aacaattctt catcggtgag
    22801 aacgaagaca agaaccgtct ctgggtggtg cccctcaata gcaactggaa aggcttgcct
    22861 gatacactcg aaactgaaag tatcgaaatc cctggctacg cagctcttct tgctgaaaat
    22921 gaaggagctc ttcgtctcaa cactgaaaat actgcccact atattaccga ctatcaagga
    22981 gacttgttag aagctgttct tgctgagcta gagacacttg ataacacaag caaactgcaa
    23041 attgttcaag aacgtcgttt gttggctgag gcagggcaca tttcttatgc agacttgctt
    23101 ccagttcttg ataaacttgc taaggaagag tcttatctgg tggtttcagc tgtttctcaa
    23161 gtgatttcag cccttgagcg ctttattgat gaaggaacgg atgctgaaac agccttcaaa
    23221 ggactggttg ctaaattggc tcgtcataac tatgaccgtc ttggttttga agctaaagac
    23281 ggagaatcag atgaggatga attggttcgt cagctggctg tttcgatgat gattcgctca
    23341 aatgatgcag aagctcgtca agtcgctagc caaatcttcg caacacacaa ggagaatctt
    23401 gcagaactcc cagcagctat tcgttcacaa gttctgatta atgagatgaa acatcatgag
    23461 actaaagact tgttagcact ttatcttgat acttatactc acgcaacaga tgctgtcttt
    23521 aaacgccagt tggcagcagc tcttgcatac agtacagatg cggataatat ccaaaacttg
    23581 attacttctt ggaaggacaa atttgtggtc aaaccacagg acttgtctgc ttggtattac
    23641 caattcctag ctcatcaagc aactcagaaa acagcttggt cttgggcgcg tgaaaactgg
    23701 gcttggatta aggcagccct tggaggagat atgagctttg atagctttgt tatccttcct
    23761 gctcatgtat ttaagactca gcaacgcttg gcggaataca aggagttctt tgagccgcaa
    23821 ctttctgacc ttgctcttag ccgtaacatc ggtatgggaa tcaaggaaat tgcagcacgt
    23881 gttgacttga ttagccgtga aaaagctgca gtcgaagcag ttgttcttca atacggaaat
    23941 gcataaataa gcctaaaata aaaa
//
LOCUS       DBERYS010000031        23047 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.031, whole genome shotgun sequence.
ACCESSION   DBERYS010000031 DBERYS010000000
VERSION     DBERYS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.031"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            60..929
                     /locus_tag="ACL8LA_001376"
     CDS             60..929
                     /locus_tag="ACL8LA_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS1813127.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="ACL8LA_001377"
     CDS             933..1889
                     /locus_tag="ACL8LA_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS1813128.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="ACL8LA_001378"
     CDS             1889..2653
                     /locus_tag="ACL8LA_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813129.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="ACL8LA_001379"
     CDS             2653..3363
                     /locus_tag="ACL8LA_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813130.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3777..4433
                     /locus_tag="ACL8LA_001380"
     CDS             3777..4433
                     /locus_tag="ACL8LA_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGS1813131.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            4541..5636
                     /gene="prfB"
                     /locus_tag="ACL8LA_001381"
     CDS             join(4541..4612,4614..5636)
                     /gene="prfB"
                     /locus_tag="ACL8LA_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGS1813132.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5654..6346
                     /gene="ftsE"
                     /locus_tag="ACL8LA_001382"
     CDS             5654..6346
                     /gene="ftsE"
                     /locus_tag="ACL8LA_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGS1813133.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6339..7265
                     /gene="ftsX"
                     /locus_tag="ACL8LA_001383"
     CDS             6339..7265
                     /gene="ftsX"
                     /locus_tag="ACL8LA_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGS1813134.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            7551..9731
                     /locus_tag="ACL8LA_001384"
     CDS             7551..9731
                     /locus_tag="ACL8LA_001384"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGS1813135.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9783..10598
                     /locus_tag="ACL8LA_001385"
     CDS             9783..10598
                     /locus_tag="ACL8LA_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS1813136.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            10738..12081
                     /locus_tag="ACL8LA_001386"
     CDS             10738..12081
                     /locus_tag="ACL8LA_001386"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1813137.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12297..13487
                     /gene="metK"
                     /locus_tag="ACL8LA_001387"
     CDS             12297..13487
                     /gene="metK"
                     /locus_tag="ACL8LA_001387"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGS1813138.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSEEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIKK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            14040..14975
                     /locus_tag="ACL8LA_001388"
     CDS             14040..14975
                     /locus_tag="ACL8LA_001388"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGS1813139.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            15009..16046
                     /gene="holA"
                     /locus_tag="ACL8LA_001389"
     CDS             15009..16046
                     /gene="holA"
                     /locus_tag="ACL8LA_001389"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS1813140.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            16219..16824
                     /gene="sodA"
                     /locus_tag="ACL8LA_001390"
     CDS             16219..16824
                     /gene="sodA"
                     /locus_tag="ACL8LA_001390"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGS1813141.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELTAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            16981..17511
                     /locus_tag="ACL8LA_001391"
     CDS             16981..17511
                     /locus_tag="ACL8LA_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGS1813142.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            17532..18434
                     /gene="rlmN"
                     /locus_tag="ACL8LA_001392"
     CDS             17532..18434
                     /gene="rlmN"
                     /locus_tag="ACL8LA_001392"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGS1813143.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVSCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            18434..18916
                     /locus_tag="ACL8LA_001393"
     CDS             18434..18916
                     /locus_tag="ACL8LA_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS1813144.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQPL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            18918..20459
                     /locus_tag="ACL8LA_001394"
     CDS             18918..20459
                     /locus_tag="ACL8LA_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1813145.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRVLKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(20515..21318)
                     /locus_tag="ACL8LA_001395"
     CDS             complement(20515..21318)
                     /locus_tag="ACL8LA_001395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGS1813146.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(21402..21615)
                     /locus_tag="ACL8LA_001396"
                     /pseudo
     CDS             complement(21402..21615)
                     /locus_tag="ACL8LA_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22094..22303)
                     /locus_tag="ACL8LA_001397"
     CDS             complement(22094..22303)
                     /locus_tag="ACL8LA_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813147.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            22473..22745
                     /gene="rpsP"
                     /locus_tag="ACL8LA_001398"
     CDS             22473..22745
                     /gene="rpsP"
                     /locus_tag="ACL8LA_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGS1813148.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            22765..23004
                     /gene="kphA"
                     /locus_tag="ACL8LA_001399"
     CDS             22765..23004
                     /gene="kphA"
                     /locus_tag="ACL8LA_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGS1813149.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT         6914 a         4161 c         5046 g         6926 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaacccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttggctat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac agcaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa attaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctgggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gccttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gtcgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc atcaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttggccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa catgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagaca atgttttgat tgcttttgga aaccatcaca aacaacatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa tgccagctca gaaaattgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagga acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac ttgcaatctc tgaccgagga tatgtactgg aaacagggaa aatcgtccta
     3301 tcaggaacag gaaaagaact cgcctcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcatta
     3421 tagtctggga gactagttta ggttgcagat aaagattgcg aagcaatcat caaatccagt
     3481 ggattgtttt agtcggcaga tggagattac gaagtaatca tcaatatagt ccgggggacc
     3541 tttttagtcg gtagattgag attgcaaaca aatctgcatc tacattgaaa gcttaatttc
     3601 taataattga aaaaatcgaa tgaaaaattt cttaccttca ttcacagagc tcgatttcag
     3661 agctcttttt gctagcttat tcatactttt ctgaatttcg aaaaagaaat gtaagcgttt
     3721 gatagattta caaaaagatt gtataatagg gataagaata gaaaaggaga agtctcatgg
     3781 cagttaaaga ttttatgacc cgcaaggtag tttatattag tccagataca acagtatctc
     3841 atgcagcaga tttgatgaga gagcaaggtt tgcaccgtct gcctgttatc gaaaatgatc
     3901 aattagttgg tttggtgact gagggaacca ttgcacaagc aagtccatct aaagcaacaa
     3961 gtctttctat ctatgagatg aattatcttc tgaataagac aaaagtaaaa gatgtcatga
     4021 ttcgcgatgt tgtcactgtc tcaggctatg ctagtctaga agatgcaact tatctgatgt
     4081 tgaaaaataa gattagtatt ctccctgtcg tagataacca tcaagtatac ggagttatta
     4141 ctgaccgtga cgttttccaa gcctttcttg aaattgcagg ttatggcgaa gaagggattc
     4201 gtgtacgctt tgttacagaa gatgaagttg gtgttcttgg aaaaattgtt tctttgattg
     4261 tagaagaaaa tttgaatatc tcccatacag tcaatattcc gcgtaaggat ggtaaggtga
     4321 ttatcgaagt gcaaatcgat ggatcaattg atttaccagc cttgaaagaa aaatttgaag
     4381 caaatggtat tcaagtggaa gaaatcgctc gcacttcagc aaaagtcttg taagaaggga
     4441 agcccaaagg cttctttttt catgaaaagg ggattagaga aaaagatgga aagaaatgat
     4501 aaaatatgct ataatgaaat aatgtaaaaa aggagtattt atggacattt cagtaattcg
     4561 tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc agggggtctc tttgacctcg
     4621 aagggctaga ggaggagatt gccatcttgg aaaacaagat gacagaacct gatttttgga
     4681 acgataatat tgcagcccaa aaaacgtcgc aagaattaaa tgaattaaaa aacacttaca
     4741 ataccttcca taagatggaa gagttgcagg atgaagtcga aattttattg gattttttgg
     4801 ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt agccgaactt gataagataa
     4861 tgaccagcta cgagatgact ctactcttgt cagaacctta tgaccacaac aatgccatct
     4921 tggaaatcca tccaggttct ggtggtactg aggcgcagga ctggggtgat atgttgcttc
     4981 gtatgtatac tcgttatggt aatgctaaag gctttaaagt ggaagtgttg gattaccaag
     5041 caggtgatga ggctggtatt aagtcggtaa ctttatcatt tgaagggcct aatgcctatg
     5101 gtctcctcaa gtcagaaatg ggtgttcacc gcttagtgcg aatctcacca tttgactctg
     5161 ccaaacgtcg ccatacctct ttcacatctg tagaagtgat gccagaattg gatgatacta
     5221 ttgaagtgga aatccgtgaa gatgatatca agatggatac cttccgttca ggtggtgccg
     5281 gtggacaaaa cgtcaataag gtttcaacag gtgtacgttt aacccacatt ccaactggaa
     5341 ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa tagagatcgt gccatgaaga
     5401 tgttgcaggc taagctctat caaatggagc aagagaagaa ggctgcagag gtagattctc
     5461 tcaaaggtga gaaaaaggag atcacttggg gaagccaaat ccgttcttat gtcttcacgc
     5521 cttatactat ggtaaaagat caccgaacta gctttgaggt tgctcaggta gataaggtta
     5581 tggatgggga cttagatggt tttatcgatg cttatctcaa gtggcgaatt agctaagata
     5641 gaaaggaact cacatgtcaa ttattgaaat gagagatgtc gttaaaaaat acgacaacgg
     5701 aacgactgct ctacgcggtg tttcggttag cgttcaaccg ggggaatttg cttacatcgt
     5761 aggaccttca ggagcaggga agtcaacttt tattcgttct ctgtatcgtg aagtaaaaat
     5821 cgataaagga agcctatcag ttgctggttt taatctggtt aagatcaaaa agaaagatat
     5881 cccgcttcta cgtcgtagtg ttggggttgt cttccaggat tataaattgt taccaaagaa
     5941 aactgtctat gaaaatattg cttacgctat ggaagtaatc ggggaaaatc gccgtaatat
     6001 caaaagacga gtgatggaag ttttggactt ggttggattg aagcataagg ttcgttcttt
     6061 cccaaatgaa ctctcaggtg gggagcaaca gcggattgcg attgcgcgtg caattgtaaa
     6121 taatcccaaa gtattgatag ctgatgagcc aacaggaaat ctggatccgg ataattcatg
     6181 ggaaattatg aatcttttgg aacggattaa cctacaagga acaactattt tgatggcgac
     6241 tcataatagc cagattgtaa ataccttgcg ccaccgtgtc attgccattg aaaatggccg
     6301 tgtcgttcgt gacgaatcaa aaggagagta tggatacgat gattagtaga ttttttcgcc
     6361 atttatttga agccttaaaa agtttgaaac gaaatggttg gatgacagta gctgctgtca
     6421 gttcagtcat gattactttg accttggtgg caatatttgc atctgttatt ttcaatacag
     6481 cgaaactagc tacagatatt gaaaataatg tccgtgtagt agtttatatc cgaaaggatg
     6541 tggaagataa tagtcagaca attgaaaaag aaggtcaaac tgttacaaat aatgactacc
     6601 acaaggtata tgattctttg aagaacatgt ctacggttaa aagtgttacc ttttcaagta
     6661 aagaagaaca atatgaaaaa ttaaccgaga taatgggaga taactggaaa atctttgaag
     6721 gagatgccaa tcctctctat gatgcctata ttgtagaggc aaacactcca aatgatgtaa
     6781 aaactatagc cgaagatgct aaaaaaattg aaggtgtctc tgaggttcaa gatggcggtg
     6841 ccaatacaga aagactcttc aagttagctt catttatccg tgtttgggga ctagggattg
     6901 ctgctttgtt aatttttatc gcagttttct tgatttcaaa taccattcgt attaccatta
     6961 tttcccgcag tcgcgaaatt caaatcatgc gcttggtcgg agctaaaaac agttatatcc
     7021 gtggaccgtt cttgttagaa ggagccttta tcggtttatt gggagctatc gcaccatctg
     7081 ttttggtctt tattgtttat caaattgttt accaatctgt caacaaatcg ttggtagggc
     7141 aaaatctatc catgattagt ccagatttat ttagtccgtt gatgattgcc ctactatttg
     7201 tgattggggt tttcattggt tcattgggat caggaatatc catgcgccga ttcttgaagg
     7261 tttaggtaaa atagctgctt ttatgaggag attgtaaaat ctcctttttt gctacaagag
     7321 tttttgaaaa gagatgcgca gaagaaaaga gcttccaaag aagtccccca gagaagactt
     7381 cttgatggct aatttgaact tgaaatttgg cgcactagaa attttttaga aaaaagtgta
     7441 atttttttat aaaagccttt acaaaaaaaa ttaaagtggt ataattaaat catagaaagt
     7501 gcaaacgttt tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag
     7561 atacattcaa aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg
     7621 tagttatcgc ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga
     7681 tgattaaccc aacctttgca ccacttgtca tcacaggtgg aattcttgag caaatcggtt
     7741 ggggggttat cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta
     7801 aagaacgtgc tggtggtgct ttcgccgctg gtcttgcctt catcttgatt aaccgtatca
     7861 ctggtacaat ctttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta
     7921 ctttctttgg tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag
     7981 ccttgaacat gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca
     8041 acaaatacta caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt
     8101 tcgtaccatt tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct
     8161 ggccagtagt tcaaacaggt atcaataact tcggtatctg gattgccaac tcacaagaaa
     8221 ctgctccaat tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg
     8281 gtcttcacca catgttgact atcccaatga actacacagc tcttggtggt acttatgaca
     8341 ttttaactgg tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat
     8401 gggtaacaga ccttgtaaac cttaaaggta ctgatgctag tcaatatcaa cacttgttag
     8461 atacagtaca tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga
     8521 tgggtgtgat tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag
     8581 gtatgatgat tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat
     8641 acatgttcat gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg
     8701 ccttcgctat ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct
     8761 tgactcgtac acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt
     8821 gggtaactgt tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat
     8881 tcaactacgc aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa
     8941 ccagcagcga agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg
     9001 gtggacgtgt aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta
     9061 aagatgcaga taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg
     9121 tgatgaaagg acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg
     9181 atatccaaga tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga
     9241 ctgaagcaca acaaaacact gttcacttca aagatcttac tgaggaagtt tactcagtag
     9301 cagacggtca agttgttgct ttggaacaag taaaggatcc agtatttgct caaaaaatga
     9361 tgggtgatgg atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta
     9421 ctgtgtcaag catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg
     9481 aagtattggt tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc
     9541 atgttgctga aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttggatg
     9601 ctatccgtgc agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa
     9661 ttaaatcagt taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag
     9721 tagaattgta atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa
     9781 gaatgaaatt tttaacactc aatactcaca gttggatgga gaaagaagca gaggaaaaat
     9841 tccagatttt gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa
     9901 tcaatcagga gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag
     9961 cagctgagcc tattcaccaa gaccattatg ttagactctt ggttgaaaag ttgttagagc
    10021 aagggaaaaa ttactactgg acctgggctt ataaccatat cggctatgac cgctaccacg
    10081 aaggtgtggc tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg
    10141 tggatgatcc aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg
    10201 gcaaggagct agcagttgcc agtgttcatc tctcttggtg ggataaaggt ttccaagaag
    10261 aatgggcacg atttgaggct gtcttgaaaa aattgaacaa gccactttta ctagctggag
    10321 attttaacaa cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct
    10381 tacaagacgc atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa
    10441 ttgatggctg gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag
    10501 agttagcggt ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg
    10561 atcactatgg cttgaatgct atattaaact ggaaataata actgaaaaga ggttggaact
    10621 ataaaattcc agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac
    10681 tactgtaatg gaataaaata tggtataatt gataaggtag atagaatcga ggatgttatg
    10741 tcatttacga aatttcaatt taaaaactat attagagaag ccttgaagga gttaaaattt
    10801 acaactccaa cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta
    10861 gtaggagaat caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag
    10921 caattagatg aagctagcga tagtgtacaa gcagtgatta ctgcaccgag tcgtgagttg
    10981 gctactcaaa tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt
    11041 gtggttaatt atgtgggtgg tacggataag gctcgccaga ttgagaaatt ggcaagcaat
    11101 cagcctcata ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat
    11161 ttagctattc ataaagccaa gacatttgtt gttgatgaag cagatatgac cttggatatg
    11221 ggattcttgg aaactgttga taagattgct ggcagtcttc caaaagactt gcaattcatg
    11281 gtcttctcag cgactatccc acaaaaactg caaccattct tgaaaaaata cttatcaaat
    11341 cctgttatgg agaaaattaa gaccaaaacg gttatttctg acaccattga taattggttg
    11401 atttcgacca agggacatga taagaatgct caaatttacc agttgactca gttgatgcag
    11461 ccgtatttgg caatgatttt tgttaacact aaaacacgtg ctgatgaatt gcattcatat
    11521 ctgactgctc aaggcttgaa ggttgcaaaa atccatggag atattgcccc tcgtgaacgc
    11581 aagcgaatca tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagatttg
    11641 gcagcgcgtg ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa
    11701 gacttatctt tttttgttca tcgtgttggt cgtactggac gaaatggcct accaggtaca
    11761 gctattaccc tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga
    11821 atcaagttta gtcctaagat ggtcaaagac ggggaatttc aagataccta tgaccgtgat
    11881 cgtcgtgcca accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt
    11941 aaaaagaaaa agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat
    12001 gaaaagcgcc gtaaaaccaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa
    12061 gctaaacgcc aaacatttta atagaaattg ttggagtatt gagctccaac ttttttattt
    12121 atgagaacga actatctaaa ccgaaacact acattaaaga ctgcaaattg cgattaaaaa
    12181 tggtataatg ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt
    12241 ttcctatcag tactttgtaa ctctataaca atatttttta agggggggac atttttatgt
    12301 cagagcgtaa attattcacg tctgaatctg tatctgaggg gcatccggat aagattgcag
    12361 accaaatttc agatgcgatt ttggatgcta ttttagcaaa ggatccagag gcgcacgttg
    12421 ctgctgaaac agctgtatat actggttctg tccacgtttt tggtgaaatt tctacaaatg
    12481 cctatgtgga tattaaccgt gtggttcgtg ataccattgc agagattggt tataccaata
    12541 cagaatatgg attttctgct gagacggtgg gagtacaccc atctttggtg gaacaatctc
    12601 ctgacatcgc tcaaggtgtt aacgaagcct tggaggttcg tggaaatgct gatcaagatc
    12661 cactggactt gattggagca ggtgaccaag ggctcatgtt tggatttgca gtagatgaaa
    12721 cagaagagct catgccattg ccaattgcac tcagtcataa attggttcgt cgtctggcag
    12781 aacttcgtaa gtctgaagaa attagctatc tccgtccaga tgcaaaatca caagttacag
    12841 ttgagtacga tgaaaatgac cgtccggtac gtgtagatac agtcgttatt tctactcagc
    12901 atgatccaga ggccactaat gaacaaatcc atcaagatgt gattaaaaag gtcatcaaag
    12961 aagttattcc atcttcttat cttgatgata agacaaaatt ctttatcaat ccgacaggtc
    13021 gttttgtaat cggtggtcct caaggggact caggtttgac tggtcgtaag attattgtag
    13081 atacttatgg tggctactct cgtcatggtg gtggtgcctt ctctggtaaa gatgcgacta
    13141 aggtggatcg ttcagcctct tatgcggctc gctatattgc caagaatatc gttgcagcag
    13201 accttgctaa gaaggcagaa gtgcagttgg cctatgctat cggtgttgcg caacctgttt
    13261 ctgttcgtat cgatactttc ggtacaggaa cagtagctga aagtcaactt gaaaaagcgg
    13321 ctcgtcaaat ctttgacctt cgccctgcag ggattatcca aatgctggac ctcaagcgtc
    13381 caatttaccg tcaaacatcg gcttacggtc acatgggacg tacagatatt gatcttccat
    13441 gggaacgttt ggataaggta gatgctttga aagaagcagt aaaataagat tttaagaggg
    13501 gaacgtcctc tcttttttat agtttttaac tatactggga tactgttctg aaaatccatt
    13561 ttgtgaaagt agagatttac atgtatagta gattgaaact agaatagtac acctcaactt
    13621 ctaaaacatt gttagaaatc gatttgactg tcctgattga tttctcctgt tcttgtttca
    13681 ttttactata tttctttaaa aatgataaag gttgagattt ctcctcgtaa tagataatca
    13741 tggggatatt tcaatccaaa gttttattcg ttatcacttg actattgcaa ggttttctag
    13801 agcaacagag tcatggaatg gactcatggt tgagatttct ccttgttgct tggacttcat
    13861 tcaaaagtct gttacccaag ccttgttcaa acttccaata cactagctgt ttccatagca
    13921 tgacttctgt actagacttt cttttccgaa taaatagata gaaccacaga atctagtaaa
    13981 cctagaatta aaattatggt ataatattag caataaaaga aatctggagg attagaatca
    14041 tggtatcaac gaaaacacaa attgctggtt ttgagtttga caattgcttg atgaatgcag
    14101 caggtgtggc ttgtatgacg atagaggagt tagaagaggt caaaaactca gcggcaggaa
    14161 cctttgttac taagacagcg accttggact tccgtcaggg gaatcctgag ccacgctacc
    14221 aagatgttcc acttggttcc atcaactcta tgggcttgcc aaataatggc ttagactatt
    14281 atttggatta tcttttagat ttgcaggaaa aagagtcgaa ccgaactttc ttcttatctc
    14341 tggtcggcat gtctccagag gaaacccata ctattttgaa aaaagtccaa gagagtgatt
    14401 ttcgtggtct gactgagcta aatctttcct gtccaaatgt tccaggtaaa cctcagattg
    14461 cctatgattt tgagacaaca gaccggattt tggcagaagt gtttgcttac ttcaccaaac
    14521 ctcttggaat taaattgcca ccttattttg atattgttca ctttgatcaa gcggcagcta
    14581 ttttcaacaa atatccgctc aagtttgtca actgcgttaa ctctatcgga aacggcctct
    14641 atatagaaga cgaatctgtc gttattcggc ctaagaatgg ttttggtgga attggtggag
    14701 aatacatcaa accgactgct ctagccaatg ttcacgcctt ttatcaacgt ttaaatcctc
    14761 aaatccaaat tatcggaaca ggtggcgttc tgactggtcg agatgccttt gaacacatcc
    14821 tctgtggagc aagtatggtg caggtgggaa cgacccttca caaagaaggt gtcagtgctt
    14881 ttgaccgcat taccaatgaa ctgaaagcaa tcatggtgga aaaaggctac gagagcttag
    14941 aagatttccg tgggaaattg cgctatattg actaaattaa atcgaaaaat ctgaagaaag
    15001 gagagacgat gctagccatt gaagaaagtc agaagttgac tttatcaaat ttaccgagcc
    15061 tgagcctatt tacagggaca gatcagggtc agtttgaagt gatgaagagt caagtgttga
    15121 aacagattgg gtatgattct gctgacctca actttgccta ctttgatatg aaagaagtag
    15181 tttacaagga tgtggaactg gagttggtca gccttccttt ctttgcggat gagaaaatcg
    15241 tgatattaga ttattttatg gatatcacga ctgctaagaa acgctttttg acagatgatg
    15301 agcttaagtc atttgaggaa taccttgaca atccttctcc aacaaccaag ttgataatct
    15361 ttgcagaagg aaagctggat agcaaaagac ggttagtcaa attacttaag cgtgatgcca
    15421 aggtcttcga tgcagtagaa gtaaaagaac aagaattgcg ccagtatttc caaaagtgga
    15481 gtcagaaaca aggtctgcag tttaccaatc attcttttga aaatctcctc atcaagtcgg
    15541 ggtttcaatt tagcgaaatc cagaaaaatc ttctcttttt acagtcctat aaggcgaatt
    15601 ctgttatcga ggaagaggat attgttaacg caattcccaa gaccttgcag gacaatattt
    15661 ttgatttaac tcagtttatt ctgactaaaa agatggatca ggcgcgcgat ttggtgagag
    15721 acttgacctt gcaaggggaa gatgaaatca aactgattgc agtcatgctg ggacaatttc
    15781 ggacttttac tcaggtgaag attttggcgg agtctggcca aacagaatcg cagattgcaa
    15841 gtagtttagg tagttatctg ggacgtaacc caaatcctta tcaaatcaag tttgcattaa
    15901 gagattcgag aggactttct ttgagctttc tgaagcaagc tatttcctat ttgattgaga
    15961 cagactatca gattaagaca ggtctttatg aaaaaggttt cctttttgaa aaggcactct
    16021 tacagattgc tagtcaggtc aattgacatt tgttgaaact acaacccacg tcaggattat
    16081 cttgtcgtgg gtttcttgct gatttgctta ttttattaga aagtatgtca gtgatttcct
    16141 tatctataag caaaaaactt gaaaaaagtg aatgtttgcg ttatcatttt catatagaaa
    16201 gaaaaagagg tattacagat ggctattatc ttaccagaac ttccatatgc atacgacgct
    16261 ttggaaccat acatcgatgc ggaaacaatg catttgcacc atgacaaaca ccatcaaact
    16321 tatgtcaaca atgccaatgc agctttagaa aaacaccctg aaatcggtga agaccttgaa
    16381 gccttgcttg ctgatgtaga atctatccca gctgatatcc gtcaagcact tatcaacaat
    16441 ggtggcggac acttgaacca cgctcttttc tgggaattga tgactcccga gaaaacagct
    16501 ccttcagcag aactgacagc agcaatcgat gcaacatttg gttcatttga agaattccaa
    16561 gcagccttca ctgcagcagc aacaactcgt tttggttcag gttgggcatg gttggttgtc
    16621 aacaaagaag ggaaacttga agtgacttca acagcaaacc aagacacacc aatctcagaa
    16681 ggtaaaaaac caatcttggg cttggacgtt tgggaacatg cttactacgt gaaataccgc
    16741 aacgtgcgtc ctgactatat caaagctttc ttttcagtaa tcaactggaa taaagtagat
    16801 gaattgtacg cagctgctaa ataatgatag ttggagggaa gaattgttct tctctttttt
    16861 aggttataag attctggtct gacaaaatcg tcagactttt ttcattttta tgagaaacgt
    16921 gctaactgct agaaattatg gtagaataaa gtattaagta atcgtggaaa aaggatatct
    16981 atgcgaaaag aaattgcacc tgaattatac aactataaca agtttcctgg tccggagttc
    17041 catttacacg gggacaaggt cgaaacggaa gggatagctt tttccttggt tgaaaatatc
    17101 aaggatgcct ttgatgtgac gacttttaat cagcgttttt cagaagtatt aaccaagttt
    17161 gattatatcg tgggggactg gagcaacgaa cagcttcgcc tacgaggttt ttacaaggat
    17221 gaccgaacag aagaaaaact tgaaaaaatc agtcgtttac aagactacct tttagagtat
    17281 tgtagttatg gttgtgccta ttttgtctta gaaaatgaag cccctaagcg agcatcattt
    17341 gacaagaaaa tgcgtaagac ggaagaagaa acaccttcta gaaaaggaaa gaaaccgact
    17401 caaactaaac gaaagtcgaa tgcagataag aaaaatagac gtcgtcagaa agaccaacat
    17461 tctcagaaag aggacaagga acaacgtcat tttgtcattc gtcagaagtg agtagagaat
    17521 aaggagaaga aatgaaaccc tcaattcata gtttggcaca tcaaaccatg caggaatggg
    17581 tattggagca gggagaaaag aaattccgtg cagatcaaat ctgggaatgg ctctaccgta
    17641 aacgtgtgca gtcatttgaa gaaatgacca acctttccaa ggatttgatt gctaagctca
    17701 atgaccagtt tgtggtcaat cccttgaaac aacgtattgt gcaagagtct gctgatggta
    17761 cagtcaaata tctctttgaa ttgcccgacg gtatgttaat tgagactgta ctcatgcgtc
    17821 aacactatgg tttatctgtc tgtgtgacca ctcaggtcag ctgtaatatc ggttgtacct
    17881 tctgtgcctc tggtttgatt aagaagcagc gtgacctcaa taacggtgaa atcgtagcgc
    17941 aaatcatgct ggttcagaaa tactttgctg atgaaggggt tcaggtcaat cttgccgtgt
    18001 ctcttcacgc acccaacaat gaacttcgtt caagtatcat gaaaattaac cgtgcctttc
    18061 cgattgaaaa actctttgct gctattgaat actacattga gacaacaaac cgtcgtgtaa
    18121 cctttgaata tatcatgctt aatgaagtca acgacggtgt agaacaagcc ttggaattgg
    18181 ctgaattgct caagaacatc aagaaattgt cttatgtaaa cttgattcct tataacccag
    18241 ttagtgagca tgaccaatat agccgtagtc ccaaagagcg cgtgttggct ttctatgata
    18301 cgcttaagaa aaaagggggc aactgtgtgg ttcgtcaaga gcatggtaca gatattgacg
    18361 cagcttgtgg acaattgcgc tttaacacaa tgaaacgtga ccgccagaag gcagtcgcag
    18421 cggtaaatcc ttaatgatga agaagactta taatcatatt ttggtctggg gagtcatttt
    18481 ctatagcatt tgcattgtct atttttgctt tactcctcaa gaacaatcta ccgtgggagt
    18541 gggaactcca gatattcagc ctcttggacg cctggttttt cttttgactc ctttcaattc
    18601 tctctggaaa ctgggcgaag tgagtgacat tggacaatta tgttggattt ttttacaaaa
    18661 tatcctcaat gtcttcttgt tttttcctct gattttccaa ctcctttatc tatttccaaa
    18721 tttgcggaaa acaaaaaagg tccttctttt tagttttctt gtgagtcttg gaatcgagtg
    18781 tacgcaatta atcttggact ttttctttga ttttaatcgc gtctttgaga ttgatgattt
    18841 gtggaccaac actttgggtg gctatctggc ttggctcctt tataaacgat tacataaaaa
    18901 caagataagg aattaaaatg agtattttag aagttaaaaa tctgagtcac ggttttggtg
    18961 accgtgcaat ttttgaagat gtgtccttcc gtctcctcaa gggagaacat atcggcctgg
    19021 tcggtgccaa tggtgaagga aaatcaacct ttatgagtat cgtgactggt aaaatgctgc
    19081 cagatgaagg aaaggttgag tggtccaaat atgtgacggc tggttacttg gatcagcact
    19141 ctgtccttgc tgaaagacag tcggtgcgtg atgttctccg tacggctttt gatgagcttt
    19201 tcaaagctga agctcgtatc aatgacctct atatggaaat ggctgaagac ggtgcggatg
    19261 ttgatgctct catggaagaa gtgggagaac ttcaagaccg tctggagagt cgtgatttct
    19321 ataccttgga tgctaagatt gacgaagtag cgcgtgctct tggtattatg gactttggca
    19381 tggatacgga tgtaacttct ttgtcaggtg ggcaaagaac caaggtgctt ttggcaaaac
    19441 ttctccttga aaagcctgat attttgctgt tggacgagcc gaccaactac ttggatgctg
    19501 agcatattga ttggctcaag cgctatctcc aaaactatga gaatgccttt gtcctcattt
    19561 cgcacgatat tccattcctc aatgacgtta ttaatattgt ctatcatgtg gaaaatcaac
    19621 agctgacgcg ttactctggt gactactacc agttccaaga agtttatgct atgaagaaat
    19681 ctcagctaga ggcagcctac gaacgccagc agaaagagat tgcagacctc aaggactttg
    19741 tggctcgtaa taaagcccgt gttgcaactc gtaatatggc tatgtctcgt caaaagaaat
    19801 tggataagat ggatattatc gaactccaaa gtgagaaacc aaaaccatcc tttgatttca
    19861 aaccagctcg tacaccaggg cgctttatct tccaagccaa gaacttgcaa attggttacg
    19921 accgtcctct tactaagcct ttaaatctta ccttcgaacg caatcaaaag gttgcgatta
    19981 ttggtgctaa tggtattgga aaaacaactc tcttgaagag tctcttaggc attatctcgc
    20041 caatcgctgg ggaagtggag cgtggagatt atttagaact tggttatttt gagcaggaag
    20101 tagaaggcgg taatcgccaa actcctcttg aagctgtctg gaatgccttt cctgccctta
    20161 atcaagcaga agtccgtgca gcccttgccc gttgtggttt gacaaccaaa catattgaaa
    20221 gccagattca agtattatca gggggagagc aagccaaggt tcgtttctgt ctcttgatga
    20281 atcgtgaaaa caacgtttta gtgctggacg agccgaccaa ccatttggat gtggatgcaa
    20341 aggatgagct caaacgcgtt ctcaaagaat ataggggatc tatccttatg gtctgccacg
    20401 agccagactt ttatgaaggc tggatagacc aaatatggga ttttaataat ttaacttaag
    20461 aatgagagta aaagaaatac agtaccgaat gtggatactg tatttttttg gtttttaaga
    20521 tttaaaatcg tagtctttca ccacttcgat gctgtcgatt gtgatagcag tagttggctt
    20581 gtctttttca tctttttcgg ccttagcaat cttatccaca acatccatac cgtcaatcac
    20641 ttgaccaaag actgggtgtt tgccatctag actagggttt ccaccttctt tgtaggcttc
    20701 gataattttc tgtggatact tgcttgtagg gagtttagaa gaggtatctg tagagttttg
    20761 gttgatgaag aactggctgc cattggtgtt tggttgacca gtattagcca tagcaagagc
    20821 accacggatg ttatacaaat aaggagtaat ctcgttcttg aaaccagttc ctttgtcttt
    20881 agtcttatcc ttgtcatgcc agatggactg accacctgta ccgtcccctt ttggatctcc
    20941 agtttggacc ataaagccat cgatgacacg gtggaaggta ataccgttat agtagccttc
    21001 tttggcgtga gtgaggaaat tttcaaccgc tagaggagcg agtttaggga agagtttaat
    21061 gcgaatatca ccttggcttg tgtggagaat cacttcggct tcatcttcag caacttcctt
    21121 agatagttgt gggaaattgg cgttttcgtt tgttaaagca tcgtttaact ccttggcaga
    21181 ttgggcagct actttggaac tttcttcagc agcaagactg gaatcaacat aatcatcacc
    21241 acgcagactg cgttggacgc tgctacaccc agctagggct acagtagaca gtaaaagaag
    21301 ggttgctagt tttttcattc tgatcctctc aaaaattcat ttataaccat tgtaccctaa
    21361 aaggaagtgg atttcaaata ttagtagttt acagaactgg cttagataat agttgggatt
    21421 tcatcatcat cttgaaaatc ttcaacttct tccccagtaa atggtttctg aaaagaactt
    21481 agtataaaac gggcaattgg ttcaacaatt aggaaattta gaggaagagc tgcaataaag
    21541 tttacgaacc aggcgcttag ataggtattg ggtgtgatag caatgccatt gtaaaaaaag
    21601 tccatagaag gacatgaaag tgaccagcta attcttcttc tgaaaaatgc tcatgaaggg
    21661 cttgatccag gtcgcaagat ttcattttag aaagtccagt aactcccttc ttcttcagga
    21721 tattcttttt catggtagta tttagatggt caagtgagtc aaaagaagtt tcgacaagag
    21781 catacccttt ttctaccaga gtttttagat gtttaggggc atcaatacca taagtgtact
    21841 caaagtattt tggaaaccag atcttaagaa agctcgtaaa gctagccagt tctctaaacg
    21901 ctaagaacta gctacaacac agcatttaca acacttcatg gttcacacca tggggtgttt
    21961 tttaatgatt ttaagcaaaa ttcacactta attgaaagtt atttaaatta gatgaaaatt
    22021 tattttttga aaaagtggga aaacattgat tctaagcgaa ttttgaatca tattcgaatg
    22081 ggagttttta aaatcatgtt tcagtggtaa aagtaccaaa ctggattctc catagtaaaa
    22141 tgatatctcc tgctagtgaa tcatctacta ataagtccat atttcgcttg ggaacgggct
    22201 tgggacttag aagccaagta tccaactctc gttcaggcga tatgaagggt ttgacttggt
    22261 gttctttata aaatttttct aaaatagtat tcacggatag catctttcaa acatttattt
    22321 cctattctaa caaatttcta aatactttaa gaaatttata gtctgtcaag tttttttctt
    22381 gacacatgtc agaaatctgc tataatagaa catgtgctaa atagctcagc tatttcaccg
    22441 aaataaaaaa ttaaagaaat gagactttaa aaatggcagt taaaatccgt ttgactcgta
    22501 tgggttctaa gaaaaaacct ttctaccgta tcaacgtagc agattcacgt tcaccacgtg
    22561 acggacgttt catcgaaaca gttggaactt acaacccact tgttgctgaa aaccaagtaa
    22621 ctttgaaaga agaccgcgtt cttgcatggt tggctaatgg agctcaacct tcagacacag
    22681 tacgtaacat cctttcaaaa gaaggcgtat tgaaaaaatt ccacgattct aaattttcaa
    22741 aataagttta aagtaggttg acagatggat acgattgaaa atctcattat tgcgattgtg
    22801 aaacccttaa tttcacaacc agatgcctta actatcaaga ttgaggatac accagaattt
    22861 ttggaatatc atttgaatct tgatcaaagc gatgtgggtc gtgtaatcgg tcgtaagggt
    22921 cgcactattt ctgcgataag aacgattgtc tactctgtcc caactgaata caaaaaagta
    22981 agaatcgtta ttgacgaaaa ataagaaggg cgggacggat gtctcgcttt tttgtgtgga
    23041 atagaag
//
LOCUS       DBERYS010000032        22809 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.032, whole genome shotgun sequence.
ACCESSION   DBERYS010000032 DBERYS010000000
VERSION     DBERYS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.032"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            467..1588
                     /gene="mnmA"
                     /locus_tag="ACL8LA_001400"
     CDS             467..1588
                     /gene="mnmA"
                     /locus_tag="ACL8LA_001400"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGS1813150.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1729..2184
                     /locus_tag="ACL8LA_001401"
     CDS             1729..2184
                     /locus_tag="ACL8LA_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1813151.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQYGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2194..4107
                     /gene="mnmG"
                     /locus_tag="ACL8LA_001402"
     CDS             2194..4107
                     /gene="mnmG"
                     /locus_tag="ACL8LA_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGS1813152.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4239..4388)
                     /locus_tag="ACL8LA_001403"
     CDS             complement(4239..4388)
                     /locus_tag="ACL8LA_001403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813153.1"
                     /translation="MKIKEQTRKLAVGCSKHCFEVVDRTDEVSSHTYGKATLTRFEEI
                     FEEYQ"
     gene            complement(4460..6139)
                     /locus_tag="ACL8LA_001404"
     CDS             complement(4460..6139)
                     /locus_tag="ACL8LA_001404"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS1813154.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6141..6374)
                     /locus_tag="ACL8LA_001405"
     CDS             complement(6141..6374)
                     /locus_tag="ACL8LA_001405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGS1813155.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6654..6869
                     /locus_tag="ACL8LA_001406"
     CDS             6654..6869
                     /locus_tag="ACL8LA_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813156.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7192..7344)
                     /gene="cibB"
                     /locus_tag="ACL8LA_001407"
     CDS             complement(7192..7344)
                     /gene="cibB"
                     /locus_tag="ACL8LA_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGS1813157.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7346..7531)
                     /gene="cibA"
                     /locus_tag="ACL8LA_001408"
     CDS             complement(7346..7531)
                     /gene="cibA"
                     /locus_tag="ACL8LA_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HGS1813158.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7709..8392
                     /gene="tsaB"
                     /locus_tag="ACL8LA_001409"
     CDS             7709..8392
                     /gene="tsaB"
                     /locus_tag="ACL8LA_001409"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGS1813159.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8389..8826
                     /gene="rimI"
                     /locus_tag="ACL8LA_001410"
     CDS             8389..8826
                     /gene="rimI"
                     /locus_tag="ACL8LA_001410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGS1813160.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENIFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8816..9826
                     /gene="tsaD"
                     /locus_tag="ACL8LA_001411"
     CDS             8816..9826
                     /gene="tsaD"
                     /locus_tag="ACL8LA_001411"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGS1813161.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9866..11172)
                     /locus_tag="ACL8LA_001412"
                     /pseudo
     CDS             complement(9866..11172)
                     /locus_tag="ACL8LA_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11508..12199)
                     /locus_tag="ACL8LA_001413"
                     /pseudo
     CDS             complement(11508..12199)
                     /locus_tag="ACL8LA_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12442..12996
                     /locus_tag="ACL8LA_001414"
     CDS             12442..12996
                     /locus_tag="ACL8LA_001414"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1813162.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKK"
     gene            13124..13648
                     /locus_tag="ACL8LA_001415"
     CDS             13124..13648
                     /locus_tag="ACL8LA_001415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1813163.1"
                     /translation="MDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICH
                     LKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLS
                     EIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVET
                     MRVYDELLEKSSHG"
     gene            13641..14594
                     /locus_tag="ACL8LA_001416"
     CDS             13641..14594
                     /locus_tag="ACL8LA_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1813164.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLFLKYMLLYHFGFFYCIYDNYVQLKFRK"
     gene            14604..16220
                     /locus_tag="ACL8LA_001417"
     CDS             14604..16220
                     /locus_tag="ACL8LA_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813165.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16569..16997
                     /locus_tag="ACL8LA_001418"
     CDS             16569..16997
                     /locus_tag="ACL8LA_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813166.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPSTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            17009..17758
                     /locus_tag="ACL8LA_001419"
     CDS             17009..17758
                     /locus_tag="ACL8LA_001419"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGS1813167.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            17771..18943
                     /locus_tag="ACL8LA_001420"
     CDS             17771..18943
                     /locus_tag="ACL8LA_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGS1813168.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPFGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            19339..20202
                     /locus_tag="ACL8LA_001421"
     CDS             19339..20202
                     /locus_tag="ACL8LA_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1813169.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            20488..20688
                     /locus_tag="ACL8LA_001422"
     CDS             20488..20688
                     /locus_tag="ACL8LA_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HGS1813170.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            20755..21420
                     /locus_tag="ACL8LA_001423"
     CDS             20755..21420
                     /locus_tag="ACL8LA_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1813171.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            21492..22718
                     /locus_tag="ACL8LA_001424"
     CDS             21492..22718
                     /locus_tag="ACL8LA_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1813172.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         6966 a         3885 c         4840 g         7118 t
ORIGIN      
        1 aggtgccctt gcagtcaaca caactgtaga cggatatact gtaaatgaaa acggtgaatg
       61 ggtttaagcc gattaaatta aatcatgtta agaacatttg acattttaat tttgaaacaa
      121 agataaggtt cgattgaata gatttatgtt cgtattcttt aggtacctgt cttatgattt
      181 caggaaatgt cattaaaaaa cgactccttt tctctaactt gaaaaataga ttagagaaaa
      241 tgggttgttt tatttattat agttatttga atgaagataa gaagaaggta tactcacgtc
      301 attcacataa tctgtatatt gactataagt tttaaaaaac aatttttaag ctcttccttg
      361 tcttctctaa ccaagcgtgt tataatgaat actgctcaag cgaccttcaa tcgtgaagca
      421 cacacgacct tcaatcgtga ataaacgaat agatgggaga cttaccatga gtgataactc
      481 taaaacacgt gttgtcgtgg ggatgagtgg tggtgttgat tcgtcggtga cggctctttt
      541 gctcaaggag cagggctacg atgtgatcgg tatcttcatg aagaactggg atgacacaga
      601 tgaaaacggc gtctgtacgg cgaccgaaga ttacaaggat gtggttgcgg tggcagacca
      661 gattggcatt ccctactact ctgtcaattt tgaaaaagag tactgggacc gcgtttttga
      721 gtatttccta gcggaatacc gtgcagggcg cacgccaaat ccggacgtta tgtgcaacaa
      781 ggaaatcaag ttcaaggcct ttttggacta tgccataacc ttgggggcag actatgtagc
      841 gactgggcat tatgctcgag tggcgcgtga tgaggacggc actgttcaca tgcttcgtgg
      901 cgtggacaat ggcaaggacc agacctattt cctcagccaa ctttcgcaag aacaacttca
      961 aaaaaccatg ttcccactag gacatttgga aaagcctgaa gttcgaaaac tagcagaaga
     1021 agcaggcctt tcgactgcta agaagaaaga ctcgacaggg atttgcttta tcggagaaaa
     1081 gaactttaaa aacttcctca gcaactacct gccagctcag cctggtcgca tgatgactgt
     1141 ggatggtcgc gatatgggcg agcatgcagg tcttatgtac tatacaatcg gtcagcgtgg
     1201 cggactcggt atcggtgggc aacacggcgg tgacaatgcc ccttggttcg ttgtcggaaa
     1261 agatctaagc aagaatattc tctatgtagg acaaggattc taccatgatt cgctcatgtc
     1321 aactagccta gaagccagtc aagtccactt tactcgtgaa atgccagaag agtttacgct
     1381 agaatgtacg gctaaattcc gttaccgtca gcctgactct aaggtgaccg tccatgtcaa
     1441 aggagacaag gcagaggtca tctttgcgga accacaacgc gcgattacac caggacaggc
     1501 agttgtcttt tacgatggcg aagagtgtct aggtggtggt ttgattgaca atgcttaccg
     1561 cgatggacaa gtttgtcagt acatttagat tgacaaattt tctcaatttg ctacaataat
     1621 aacagcaata gaaatgatgg tcaaagctca tggatgttgc aggctttttt gtcctgcact
     1681 tctttgtagt tttgactgtt tttgtgtcgt ttaagggaaa ggacaagaat gactcaacaa
     1741 gactttcgga caaaagtaga caatacggtt tttggtgttc gggcgacagc cttgattgtc
     1801 caaaatcaca agcttctagt taccaaagac aagggcaagt attacactat tggaggtgcg
     1861 attcaagtca atgaaagcac ggaagacgcg gtagtccgtg aagtgaagga agaactgggt
     1921 gttaaagctc aagctgggca gctagctttt gtggttgaaa atcgttttga acaatacggt
     1981 gtttcctatc acaatatcga gtttcattat ctggtggatt tgcttgaaga tgccccattg
     2041 accatgcagg aagatgagaa aaggcagccc tgtgagtgga ttgacttgga taagctccag
     2101 aatatccagc tagttccagc ctttttaaaa acagccctac cagattggga aggccaacta
     2161 agacacattc atcttgagga ataggagaga aacatgactt atcattttac tgaagaatac
     2221 gatattattg taattggtgc gggacacgct ggggttgagg cttccttggc cgctagccgt
     2281 atgggctgta aggtcctgct tgcgaccatc aatattgaaa tgctggcttt catgccttgt
     2341 aatccctcta tcggtggttc tgccaagggg attgtcgtgc gtgaagtcga tgccctcggt
     2401 ggcgagatgg ccaaaaccat tgacaagact tacatccaga tgaagatgct aaacacaggg
     2461 aaggggccag ctgtccgtgc ccttcgtgcg caggctgaca aggaacttta ctctaaggag
     2521 atgcgcaaga cggttgaaaa ccaagaaaat ctgacccttc gtcaaaccat gattgatgag
     2581 attttggtgg aagatggcaa ggttgtcggt gtgcgtacag ccacccatca agaatatgct
     2641 gctaaggctg ttattgtgac gacagggact gctctccgtg gggaaattat catcggagac
     2701 ctcaagtact catcaggtcc taaccacagc ttggcttcta ttaacctagc tgacaatctc
     2761 aaggaactgg gtctcgaaat cggtcgtttc aagacaggaa cccctccacg cgtcaaggcc
     2821 tcttctatca actacgacgt gacagaaatc caaccaggag atgaagcgcc taatcacttc
     2881 tcatacactt cacgtgatga ggattatgtc aaggaccaag tgccatgctg gttgacctat
     2941 accaatggta ccagtcatga gattattcaa aacaacctcc accgtgcgcc tatgtttaca
     3001 ggtgtggtca agggagtggg gcctcgttac tgtccgtcga ttgaagacaa gattgtgcgc
     3061 tttgcggaca aggaacgtca ccaactcttc cttgagccag aagggcgcaa tactgaggaa
     3121 gtctatgttc aaggactttc aaccagtctg cctgaggatg tccagcgtga cttggttcat
     3181 tccatcaaag gtttggaaaa tgcagagatg atgcggacag gttatgctat tgagtatgat
     3241 atggtcttgc ctcatcagtt gcgtgcgact ttggaaacca agaaaatctc aggtctcttc
     3301 actgctggtc agacaaatgg aacatcaggt tacgaagaag ctgctggcca agggattatc
     3361 gcgggtatca atgcggctct gaaaatccaa ggcaagcctg aattgatttt gaagcgcagt
     3421 gatggttata tcggggtgat gatcgacgac ttggtgacca agggaaccat tgaaccctac
     3481 cgtctcttga ccagtcgtgc tgaataccgt ctcattcttc gtcatgacaa tgctgatatg
     3541 cgcttgactg agatgggacg cgagattggc cttgtggacg atgaacgctg ggctcgtttt
     3601 gaaatcaaga aaaatcaatt tgataatgag atgaagcgcc tagacagtat caaacttaag
     3661 ccagtcaagg aaaccaatgc caaggttgag gagatgggct tcaaaccctt gaccgatgca
     3721 gtgacagcca aggaattcct tcgccgtcca gaagtttctt accaagatgt ggtggccttc
     3781 atcggaccag ctgcagaaga cttggatgac aagattatcg aattgattga aacagaaatc
     3841 aagtatgaag gctatatttc caaagccatg gaccaggttg ccaagatgaa acgcatggaa
     3901 gaaaaacgca ttccggccaa tatcgactgg gatgacattg attctatcgc aaccgaagcc
     3961 cgtcagaagt tcaaactcat caatccagaa accatcggcc aagccagccg tatttcggga
     4021 gtaaacccag cagatatttc tattttgatg gtgtatctgg aaggtaaaaa tcgtagtatt
     4081 tctaaaactc ttcaaaaatc aaaatgatac gtcgtcggct tcttacgaat gagttcaaag
     4141 cttggctttg attcatctcc agcctcccat agtttcccga actatgggag ctaactcaag
     4201 ttatgctttc gcttagcctt ctagtctgat ttcgattttt attgatactc ttcgaaaatc
     4261 tcttcaaacc gcgtcaacgt cgccttgccg taggtatggc tactgacttc gtcagttcta
     4321 tctacaacct caaagcagtg ctttgagcaa cctacggcta gcttcctagt ttgctctttg
     4381 attttcattg agtatgagtt ttaatctcaa aagaaagcat agagaaaaac agctccgagg
     4441 acggggctgt tttcttgctt tattcttcat ctggtgtgag gatcatcggg atgatgatcg
     4501 gttcacgttc ggtattttca tagaggaagg ggcgaatagc gttgacaatg gcaccattga
     4561 cagattgcac gctggcatcc ttatttttca gtgcgatacg aatggcattg aagaggatac
     4621 gctggctttg gcgaatcaag tcgccagact ctctcatgta gacaaagcct cggctgagga
     4681 tgtctggacc agacagaatc atctgcgatt tgaagtcaac agttgcgact gccagaacga
     4741 caccgtcttc agatagatcg cgacgatctt tgaggacagc tgcgccaatt tcaccgatac
     4801 gatttccatc gacatagata tcttgggcgt tgaaatgacc tgcgatacga gctgagtcag
     4861 cagtaagggc aagcacatcg ccattgctca tgataaagat attgtccttc tcaacaccag
     4921 tatccactgc tagtccagcg tggacttttt gcatgcggta ttcaccgtgg acaggcatga
     4981 agtattttgg cttaatcaag cagagcatga gtttttgctc ttgctgacca ccgtgtccag
     5041 atgtatggat attgttcact ttaccgtgga taacttcgac accagcttca gaaatgatgt
     5101 taatcagctt gttgacgcta gtagtgtttc cagggatggg actagaagag aagataacgg
     5161 tatcacctgg ttgtaattgt acttgacggt gggttccgtt ggcgatacga gagagagctg
     5221 ccataggctc accctgacta cctgtacaga ggataagaac ttctcctgca ggataatctt
     5281 tgatttcatt tggctcgata aaggttccct taggagcttt gatgtagcca agatcgattc
     5341 cgttgacaat ggccttttcc atagaacgac caaagaccgc aatcttgcgt ccagtcttaa
     5401 cagcagcttc tgttgcctgc tggagacgga agatatttga ggcaaaggat gcaaagatga
     5461 tacgtccttc aataccttgg ataatcttca taatggactg accaacgact ttttcagagt
     5521 tggtaaaggt tggtacttcc gcatttgtcg agtcagacag gagacagagc acgccttctt
     5581 caccaagcgc agccatacga tgcaagtccg caggttctcc aactggagta aagtcgaact
     5641 taaagtcacc cgtacagacg attttccctt gaggagtatg aatgacaatc cccaaaggct
     5701 ctggaataga gtgagtcgtt ctaaagaaag ttgccttgag atttttaaag gtcaactcgg
     5761 tgttgtggtt gatttcgtaa agtttggcgt tgcgcaagag gccgtgttct tcgagtttcc
     5821 cacggatcaa agccaaggca agcggtccag cgtagatagg gacatttgct tgcttgagta
     5881 ggaacggaat cccaccaatg tggtcctcgt gtccgtgtgt gattaaaaca gccttgacgc
     5941 ggtcgatatt gtccacgatg taagagtagt caggaatgac atagtcgata ccaagcaagt
     6001 catcttctgg gaatttaatc ccagcatcga cgatgataat ctcgtcttgg tattcaattc
     6061 cgtaagtgtt tttcccgatt tctcccagac caccgatggc aaaaacgccg acttcttcag
     6121 gtttaagagt ataggccata ttagaactcc gtgatttcga aggcgccagt ttctttttcg
     6181 taatcgagca atttgtcaga caagagttcg atatattcga tattgtactc tgggcgattt
     6241 tcttcgacaa gttggcgagc agtgatgcga ccctcaagtt ctgagctggc atcgatgtct
     6301 aggtaaagtg cgcgtgttgt ttcacggcgt gggctacgtt cttttgtttc ttgataaaaa
     6361 actttgtaaa tcatatagtt cctttctatt tacaggtcag cttattccaa acttttagca
     6421 gatatttgct tgatttcatt ccattttgtg tctagattga ccttgattcg ttacaattat
     6481 ttcaattata ccacaaaatg aaaaattagt aaaagacgga aacgagaaag ttaggggacg
     6541 atttctttgg taagcgtttg caagaataaa gaaaatgtat tatgttgata acggaagtga
     6601 aatttattgc ttgtcataat tccatataga ttttaagtca aggaggttgc cctatgtata
     6661 acttatttgc ttttgctatc ggagctcgct tggtagcttt tattgttttt gttgcttgcg
     6721 tttatgcggg gaatcttcta tttgccaaac tggaacgacg acaaaccaag ttcttgtgga
     6781 aaatgacctt tgtgactctc tactcgcttt ttctcctcct cgtgacctat gtcgtttggt
     6841 ttgtatttta ctttgaatta aatgcttaga tgccctgctt atgctggggt tttttgtttg
     6901 gggatgtaga gatagtagat ttacgacacg aaaattccac cccatctatc aagatgaggt
     6961 ggtctttgca tttttctaac atgacatgac aatggaattt tcaaagctta ttatttttat
     7021 gggagtcatt gttctgatca aaaatagagt tgaagcataa aaataaaaca atacaaacag
     7081 ataaatcgaa tggtaaaggg atggtagtaa tgttcaaatc attgagaatg ctgagcaaac
     7141 tgtaagagat gaagacaata aaatttatcc ttctaaaaat gtgtttctta tctagcatga
     7201 cttaccaaac tttttacgaa ggtcttttcc aatggtataa cctgccataa ataaagcgat
     7261 accaacaccg acaccaaagc cacccttgat ttcttgcaat tccttattag aaatttcacg
     7321 ataattgttc aaatctttca tcatattaat ctcctcctaa aaaataacca gctgccaaac
     7381 cagaacctat aagtgctgtt ccacctgcaa tagccttcca agatactgct actccaaaga
     7441 taaccaaggg agcgagaccg ccatcaatat ctgataattc attttcagat aaggataaaa
     7501 attgattgtc aagaatgtca aaatttgtca ttgaactttc ctcctttttt cttgttcact
     7561 tttatattcg gaaaagaaaa ggaaaagtga gcattttttt aaattatttt tagttaagtg
     7621 agggcactct tgtctggggg caaagaaaat tcccatgtgc cagcttttgt agtataatag
     7681 taagcagaca aaaagatagg aatgtgttat gaaagtatta gcttttgata cgtccagcaa
     7741 ggctctttct ctggctattt tagaggataa gcaggttctt gccgagacga cgattaatat
     7801 taagaaaaat cacagtatta ctcttatgcc tgccatcgat tttttgatgg caagtttgga
     7861 ttggacaccc aaggatttgg accgaatcgt ggtagctgaa gggccgggta gctatacagg
     7921 cttgcgaatt gcggtagcaa ctgctaagac cttagcttac accctgaaca tcgagttggt
     7981 tggtatgtcg agtctcttgg ctctggtgcc ccaccaacaa gaaggtttgt ttgtcccctt
     8041 gatggatgcg cgtcgcaata atgtttatgc aggattttat gaaaatgcca aacctgtcat
     8101 ggcagaagcg cacctatctt ttgaagaggt gctagaaaaa gtcaagggta ctagtcaggt
     8161 aacctttgtc ggagaagttg gcccctttgt tgagcagatt caaaaacact tgccaaggac
     8221 tgattacaaa gaaacattgc ccaatgcagc caatcttgct cttttagctt gggacaagga
     8281 agcagactcc ttgcatgatt ttgtgccgaa ttacctcaaa cgagtcgagg ctgaggaaaa
     8341 ctggctcaag aaccataccg agtctggtga gtcctacatt aaacgcctat gatagaaatc
     8401 aagcgaattc aacaacagcc tgacctagct caagccatct acgctgttat ggcagctgtt
     8461 tacctagtca gtccttggac tctggagcaa atccaagcag acctgtccca agaccagact
     8521 tggtatgttc tggcttatga tggggcagaa gtgattggtt ttctagctgt tcaggaaaat
     8581 atctttgagg cagaagtctt gcaaatcgct gttaaaggag cttatcaggg tcagggaatt
     8641 gcgtcagcct tgtttgctca attgccgaca gataaggaga ttttcctcga agtcagaaag
     8701 tcaaatcaac gagcgcaagc attttacaag aaagaaaaaa tggcggtcat cgctgagcga
     8761 aaggcctact accatgaccc agtcgaggac gccattatca tgaagagaga aatagatgaa
     8821 ggatagatat attttagcat ttgagacatc ctgtgatgag accagtgtcg ccgtcttgaa
     8881 aaacgacgat gagctcttgt ccaatgtcat tgctagtcaa attgagagtc acaaacgttt
     8941 tggtggcgta gtgcccgaag tagccagtcg tcaccatgtc gaggtcatta cagcctgtat
     9001 cgaggaggca ttggcagaag cagggattac cgaagaggac gtgacagctg ttgcggttac
     9061 ctacggacca ggcttggtcg gagccttgct agttggtttg tcagctgcca aggcctttgc
     9121 ttgggctcac ggacttccac tgattcctgt taatcacatg gctgggcacc tcatggcagc
     9181 tcagagtgtg gagcctttgg agtttccctt gctagccctc ttggttagcg gtgggcacac
     9241 agagttggtc tatgtttcgg aggcaggaga ttacaagatt gttggggaga cacgagacga
     9301 tgcagttggg gaggcttatg acaaggtcgg tcgtgtcatg ggcttgacct atcctgcagg
     9361 tcgtgagatt gacgagctgg ctcatcaggg gcaggatatt tatgatttcc cccgtgccat
     9421 gattaaggaa gataatctgg agttctcctt ctcaggtttg aaatctgcct ttatcaatct
     9481 tcatcacaat gccgagcaaa agggagaaag cctgtctaca gaagatttgt gtgcttcctt
     9541 ccaagcagca gttatggaca ttctcatggc aaaaaccaag aaggctttgg agaaatatcc
     9601 tgttaaaacc ctagttgtgg caggtggtgt ggcagccaat aaaggtctca gagaacgcct
     9661 agcagccgaa atcacagatg tcaaggttat catcccccct ctgcgactct gcggagacaa
     9721 tgcaggtatg attgcctatg ccagcgtcag cgagtggaac aaagaaaact tcgcaggctg
     9781 ggacctcaat gccaaaccaa gtcttgcctt tgataccatg gaataaagag tggctctttg
     9841 tcaagtgtag tgggtaggcg aaaagctaca atctggagag tacgaaattc gtactctctt
     9901 ttttgatgtt caaagtcatc aaaatccgtt ttttaaaatt gttaaagttc cggaaaccaa
     9961 aggctttgcg cttgataacc ttaatcaaat tattggtcgc ttcaagttta gcgttggaat
    10021 aaggcagctt aagtgcgttg ataatcttat ctctatccct taaaaaagtc caaaagaccg
    10081 tttgaaaaag cggatgaacc gttggcagtt cttgctcaat caatccaaag aaatggtcgg
    10141 cattcttctc ttgaaaatga aagagcagga gttgatagag ttcgtagtga acctgtaatc
    10201 cctcagagta agaaagcagc ttatctcgta cttcccgatg ggttaagtgc attcgaaacg
    10261 tgggctgata gaatcgtttc tgacagagct tgaagctatc ctggtgtagt aaatcagtcc
    10321 agtggactga tttagcccga gcctagaaac gaaaaagcga gggttccaaa agcgcttgag
    10381 cgcccgatac tccaaggatt ttcggtcaaa ttggttcatg atttggattc gtactcggtt
    10441 catagctcgg ctcagatgtt ggacaatgtg gaagcggtca agaacaatct tcgcctttgg
    10501 aaagagttgc ttagcgagcc gataataagg gctgtacatg tccatggtga tgacctcgac
    10561 cagctcccga acctctctct gatagcgttg gaaatggttt cgaatcaccg catgagttcg
    10621 cccgtctaaa attgcgagga tggatttgga ctcaaaatct tgggcaatga agctcatttt
    10681 gctctttttg aagctatact catcccaact cataactttt ggcaacttgg tccaatcggt
    10741 ttcaaactta aattccctca gttttcggat gacggtggaa gttgagaccg ccaatctgtg
    10801 ggcaatctca gtcatagact gcttttcaag gaggagctga gcgatttttt gtctcaccat
    10861 gttggaaatc tggcaatttt tcttgacaat ggatgtttga gaaacgacca ccttaaggca
    10921 attcttgcac tgaaagcggc gctttttgag atgtagtacc gtgggtaaac cctgacagtc
    10981 gagaagcgga attttagagg ctttctggaa gtcgtatttg atcatcttcc cttgacaatg
    11041 aggacaagga ggagcggggg aatccaactt tgcctgaacg actagatggg tttggtattt
    11101 cagaacagac aagattttga tatttttgtc tttcaatccg agcaaatctg tggtattctt
    11161 aagttgttcc atatgaaccc tttctaatga gttggttaga cgcttttcat tataggtcat
    11221 atggggcttt ttttctacaa gaaacgaccc tataattcct ggggtgggat tacccactac
    11281 agaaattata gagccaaaac attccaaagt cttgtctgac tctggaatgt tttctgttca
    11341 cagttgtttt gtaactgcct aataagtaac cgcgctataa cgaggtatct accgagattg
    11401 tcaattctcc agtatactgt tataaaaaac aaaactggag gattttttta tgtcccccca
    11461 tcgtcccatc gactgttccg tagctgccca ataacggagt atagtcggct gaaacaagaa
    11521 taggacaaaa gtgcctcaag aaaagcatcg taatttgaca atattattct gagatgtttt
    11581 tgatgtaagc ccatattttc tcaatgggat tatactcggg tgaataggta ggaagtggta
    11641 acagtctatg ccctgctcct tacacatgtt cattctgtga aaccttgcat tgtccataat
    11701 gataagggat ggtgtatcta aagtaggtag taagaatttt tggaagcaag cttcgaaaaa
    11761 gtcactcgtc atcgtatctt tataggtcat cggagccata gtatattgaa acaaaaaagg
    11821 gcattagtac ttctataaca gtatcgcaat ctctggtgtg cgttgtctcg atcttaattc
    11881 aaataattat atcttcttcc agagagacta actgcctgct caaggctcga tcatattcga
    11941 gataaaaata ggtctcaacc cctgtctcat aaatataaat aggagtcagg tagcttaagt
    12001 gattcaattc tttaaggaac cgatttactt tttctgggtc ttgttcgcag taggtacagc
    12061 tcttttttag actatatccc atagccttta gagcgtaatg aatagttgtt ggaggacagt
    12121 tgaattcagc agctatttca gtcaaataag catcgggatg agtttcaata tagatcttta
    12181 atttatctct atcaactttt tcaattaata gtggaagttt tcttaaagtt agtgtgttga
    12241 aacgttttta tacagtgcta aaatttgaag tgtagtgcag atgtatttgt aatagtatta
    12301 tagcaattct ttggagaagg tcgagacaag agaaaatagg tatctttgag atataagcat
    12361 gatttactat aaaattattg agttattcat aaaaatttct ctatcaaata tcataatgcg
    12421 tcgtttaagg agatggttac aatgcaatat agttgtggaa aaataaatat taatatccct
    12481 gatggatatg gagacattaa ggatatagtg ttttcggctc atattatagt taggtataat
    12541 aatggacatt gtggaggtat agatccgcat ataattggac tttgtaaaaa gcagatcaga
    12601 aggatgtctc tatatcctat tttgataatc gtatctagag attcaaaggt tattgatgat
    12661 tataaaaatt tagatattgc ctatgttgat tgtactcaat gttcaaataa ttttgaaact
    12721 gccttacacg ttaaaaatat tttaaaatta ttaaaaatcc aacttataca ctgtcatggc
    12781 tactcaacta attatttttt atatatgtta aaaaaacttg acaagaatgg atttggaaag
    12841 gttaagacag tgataacatg ccatggatgg gttgaatata acttaaaaaa gaaattcttg
    12901 acttattttg atttttggac atattctatg ggagatgctt ttatttgtgt atctgaaact
    12961 atgaaaaaaa gattggagag tataataaaa aaataagaag atagttgtaa taaataacgg
    13021 aattaatgta tcaaatagcg atttagatgt agtaggtgtt caagacttta aaaaagaatt
    13081 ttgtattcca aataacaaaa aaatcatttg ttatgttgga aggttggatc cagaaaaaag
    13141 gccggataga ttccttgaat ttgcagaaaa attattttta gtcagagaag atgtgatttt
    13201 tattatggct ggaaatggta gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa
    13261 atgtagagat aatttcaggt tacttggtga aatctatcca gctactatag tttaccaaat
    13321 atcggattta ttgtatattc cttctgatac agaaggaata cctatgtgtg ttttagagtc
    13381 aatgtcacaa ggaactccgg ttttagctag taatgttggc gggttaagtg aaattattga
    13441 acatagggtt gatggatttt tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc
    13501 taatttttta ctcaatgatt ctgagtattt gaaatatata ggtgagaata gtaaatcaaa
    13561 aataagaaaa catttttctg tgcaaaaaat gtttgtagaa accatgagag tatatgatga
    13621 attattagag aagagtagtc atggatagga atatagatca agaattagta agtattataa
    13681 taccaacaca taataggtat gaatctctga ttagagctgt gaaaagttgt ttacatcaaa
    13741 gttataaaaa tatcgaagtt ataattattg atgataatta tagtaatgta aatttaagaa
    13801 ataaaattat ccatcaattt ggctatacca atcatagaat taagttaatt ttaagtaatg
    13861 aagatttagg tgcaactaat gccagaaaca taggtatcaa aaattctaga ggtaagtata
    13921 tatcattttt agacgatgat gatgaatata tgtcagatcg aattttaaag ttgatggctt
    13981 gttttaaaaa gagtagaatg aagaatttag ctttagttta tagttatgga ataataattt
    14041 atccaaatgg tacacgagaa gaggagaaga ccgattttgt tggaaatccc ttgtttgttc
    14101 aaatggttca caatatagca ggtacgtcat tttggttgtg taaaaaagag gtgctagaat
    14161 taattaatgg ttttgagaaa atagattcac atcaggacgg tgttgtttta ttaaaactac
    14221 ttgctcaagg ataccaaatt gatatagtgc gagaattctt ggtgaattac tacgctcaca
    14281 gtaaagaaaa cggtatcact ggagtgacac aaaaaacaat taatgcagat gaagaatatt
    14341 ataattactg taggaaatat tttaatttat tgagtttcaa cgagagaata ttggttacaa
    14401 agaaatatta ttctttaaac ataaagcggt tactattaat aggagacaaa tgcaaggctt
    14461 taaaagtaat caagaaggca agagaagaaa aaatttttaa cgaatttctt tttttgaaat
    14521 atatgttatt atatcatttt ggttttttct attgtatata tgacaactat gttcaattaa
    14581 aatttagaaa gtgagaaact attgtgtata ctattataaa atcaaatata aaaaaattta
    14641 gtttattaac gatatttatt gttgctggtc aattattgct aatttatgca gcaactatta
    14701 atgctctggt gttgaatgaa ttaattgcga tgaatttaga gcggtttttg aaattgtcaa
    14761 tctaccaaat gattgtctgg tgtgggataa tattccttga ctgggtagtg aaaaattatc
    14821 aggttgaagt gatccaagag tttaatctag agattcgaaa tagagttgcc acagacatct
    14881 ctaactctac ctatcaagaa tttcatagta aatcatcagg aacatatctt tcgtggctaa
    14941 ataatgatgt tcagacttta aatgatcagg cttttaaaca acttttttta gtaataaaag
    15001 gaatttctgg tactatattt gcagttgtga ctcttaatca ctatcattgg tcattgactg
    15061 tagccacctt gttttcatta atgattatgc tacttgtacc aaaaatcttt gcatcgaaaa
    15121 tgcgagaagt tagtctaaat ttaactaacc aaaatgaagc ttttttaaaa tctagtgaga
    15181 ctatattgaa tggatttgat gtgttagcat ccttgaatct tttatatgta ttgcctaaga
    15241 aaattaaaga agcaggaatt ttattaaaga tggttataca aagaaagaca actgtagaaa
    15301 cgttagcagg cgctattagc ttctttctca atattttttt tcagatatct ctcgtttttt
    15361 taacaggcta tcttgcaata aaaggaatag tgaaaattgg tactattgaa gcaataggag
    15421 cactaacagg tgttattttt acagcgctag gtgaattagg aggtcaatta tcctctatta
    15481 ttggtacgaa gcctattttt ttaaaattgt attcaattaa tccaattgag tcaaataaaa
    15541 tgaatgatat cgaaccaaat gaggtgaata gagattttcc gttatatgaa gcaaaaaata
    15601 tttgctataa gtatggagat aaagaaatat taaaaaactt aaatttttgt tttcaacgta
    15661 atgaaaagta tttaatttta ggtgaaagtg gaagcgggaa atctacatta ttaaaattat
    15721 tgaatggctt tttgagagat tatagtggag aattgcgatt ctgcggggat gatataaaaa
    15781 aaacctccta tttaaatatg gtttcgaatg ttctatatgt agatcaaaaa gcttatttgt
    15841 ttgaaggtac gattagagat aatattttat tggaagaaaa ttatactgat gaagaaatac
    15901 tacagtcttt agagcaagtt ggtttgagtg taaaagattt tcctaataac attttagatt
    15961 attatgttgg tgatgatggg agattactgt caggagggca gaaacaaaaa attactttag
    16021 ctagagggct aattagaaat aagaaaatag tattaattga cgagggaact tctgctatcg
    16081 ataggagaac ttcgttagcg attgaacgta agatattaga tagagaggat ttgactgtca
    16141 ttattgttac ccatgctccg catccggaac ttaaacaata ttttactaag atatatcaat
    16201 ttccaaagga ttttatttaa gaatataggg gagggaaaca tgagattcta ttgtgaagct
    16261 tatgaggtaa ttactgaaga gaaaaaagta tactttaatg atattgagtt agaagttaag
    16321 tattcctctg tcgaagaatt gtttataata tgtgagaaat tattagataa gaaaaaagta
    16381 tcattttttt atgtagatga gaaaccctta aggtatctgt tatttgacta tatatttcta
    16441 ctagttctag ctaaaaaaat atcccaatct tatgaactgc accccaaaag ttagatttat
    16501 tctgtctaac ttttggggtg cagttcattt tgcatagttt tggcattcat cctagatatc
    16561 catataaaat gtctgagatt gttgaaatgt ctaaaaaaat atcagatcta aacgataaaa
    16621 aagaatttca caaaaagaac tgtgagttga ggtttcaaga tagtgttctt gataacataa
    16681 atagtgtccc gagtacaact catttattta gttgtttaaa tattcaaaca ttagtaaaaa
    16741 ttattttata tatttttcga tacaacagtt gtatgaaaat atttaatttt agagataaag
    16801 agcttatctt ttttaataag attgtaaatg ggctgataca aaatattgag gagaatttag
    16861 aggatgatat tgaaaggata ttgaaatatt tatatatatg tttatttaac gaaatattca
    16921 ttattaagaa taaggtgaat ttttttgatg atgtggaatt taatcagaca cttagtgagt
    16981 ttttagataa attgtagagg gaatggttat gagtaacaat actaagtata tctttttatc
    17041 tccacattta gatgacgcaa ttttttcctg tggtgattat atttcaaaat taacttcaga
    17101 aggagaaatt gtacttgtta ttactatatt ttctggttac cctttgagcc aacagctaca
    17161 gccctctgct aagcaatttc ataagttgtg taaccttggg aaatatccga ttgaagaaag
    17221 gaagaaagaa gacaggttag cttgtgaacg tctacagtgt gattttagac acttatcgta
    17281 ctatgaatgt ctctatagga aagatagaaa tggcaatttt ctataccgtc atatctatag
    17341 tgaattaaaa aatgaggaca cattaaagaa tgatatcata aaagagctgt taatgcattt
    17401 agacgacaaa tgtgtcgtat attgtccgct ttcacttggt gaccatgttg atcatgtttt
    17461 tgtgaatagt attggaaggg cgcttgaatt tatgcgttat aaagtaatat attatgagga
    17521 cttcccatat gtaagtgata gtagtatggt tagttatatg ggaaaaacta aagaattaaa
    17581 aatgtatcaa gaggaacttg atgaaaaaca ttatattgat agaatatcat caatattgtg
    17641 ctataaatct cagattttga taatctggaa atcagttgaa aaacttttaa acaatattaa
    17701 agagttgtat ttaaggaatg gggcagctta ttctattaga ttttggatta agaaataagg
    17761 aagtagtttt atgaatatag cagtaattgg tttggggcat gttgggctgg cctacgcgtt
    17821 actatttgca tctaaatata aagttgttgc atatgatata gattctgtaa aaataaataa
    17881 tttaaaaaag ggcattcttc catctaaaaa tgaagagctt atgaagtttt tttgcgagaa
    17941 taacttaaat attacttttt ttgatacatt ttctgaaatt aaaaataata ttgattatta
    18001 tattattgcg cttccgacag attatgatga gaaaattggt agttttaata catatgaaat
    18061 cgaacaaacg gtatcgaaga ttctgagggt aaaacctaat ggaaagatta ttttaaagtc
    18121 aacagttccg ttcggctttt caaacaaatt aaaaaggctg tttgatacaa aaaatatcat
    18181 ttttgtccct gaatttttga gagaaggttg ttctatatat gataatttat atccaagtcg
    18241 catagttgtt ggagatgaga cagttgaagg aagaaaaatt gcagagttgt tcctttcgat
    18301 tagtactcat agtactgcca atattaaaaa tgttatgtta gtttctccta ctgaagcaga
    18361 agcaattaag cttttttcta acacattctt agctctccgt gttgcttttt ttaatgaact
    18421 agattctttt gctgagagga gaagtttaaa tgctgaagtt gtaataaaag gtgtttgttt
    18481 agatccaaga attggaaatt tttataataa tctttctttt ggatttggag gatattgtct
    18541 tcccaaagat actaaacaat taaaaaaaga atttatagaa ataaatgccc cagtgataga
    18601 agcgattgat atttcaaaca caaatagaaa acagttcata gttaaacaaa tattggaacg
    18661 aaagccaaag atagtaggaa tatataaatt ggggatgaaa tataattcag ataattacaa
    18721 agagtcagct attttaagta taattaatga actattgatt gtcggtataa aaattttagt
    18781 atatgaacca aatttaaacg ttagtataga taacgtaatt tttgaaaaga attttgaact
    18841 atttacaaaa caaagtgatc taattgttgc aaatagatgg gataggggcc ttgaagctta
    18901 taaagataaa gtttacacaa gggggatctg gataagagat taactattga tattaaaaag
    18961 gcaataattt ataaaatttg tacaaaaaaa tgttaaattt tcacattttc atagtatact
    19021 gtttaataga aagtaggttc tttgtaaccg ctccattata aaatcctttc actcttccgg
    19081 tatactatta ggaaaaacat aactggagga tattttgagc tcacacctca tcgttccagt
    19141 ggctgttccc caagctcgtg tactgcccaa aaaagttaga cattctattt agaacgagga
    19201 ttgagttctg tattgtacaa ggctcggtcc ttttagagtc agcttaaggc tggctttttt
    19261 attttttcta actatcagaa tattttgaca aatgaacaca aataatgata taatatgcaa
    19321 aagctaggag gtggtaggat gattgaaaaa atggaattgg gggaatttta caaggaatta
    19381 cgtttggcga gaaagctcaa gcagtcagat gtggcttgtg acgggctaac agcttctcag
    19441 ttgtccaagt ttgagctagg gcagtccatg ttgtctgctg ataagctcat attagccatc
    19501 caagggatca atgtgacctt tgatgagttt gggcacaagc tcaacaacta tcaagaatct
    19561 ccacatatgc agattggtag aaaggttgtg gatcgctttg ctcatcaaga tatagctggc
    19621 ttagagcaac tgttggagga agtcgagcaa gaacagatgg cagagactta tcgtcgtttg
    19681 aatgctattg taatcaaaga tgccatccac tcactagata aaagctaccc actagcagag
    19741 gaggatagcg agtttttgac cacctatctc tatgctatcg agtcttggac ctggtttgaa
    19801 ctctatctct tttgcaatac tctgcccttc ttgagcaatc aagatctgat ctttttatca
    19861 acctccttac tcgaaaaatc caaagaattt aaagagttag tacacaatcg attgtatatg
    19921 aagcaaggac tcttaaatat catctcagag ctcatggagc gcaaactttt ttcttatatc
    19981 ccaatctttg aagccgagct ggagagtatg ctccgtccat acgatgtttt tgagaaagtg
    20041 ttgtggcagt ttttaaagaa gatgagcatt ttccttcaaa ctaagggaag caatcaaaaa
    20101 gagattgaac actttatcca atctctgcaa gtattagaaa atccacaatt aatagccctt
    20161 tttgaattgc gtttgcagca atacaaaaag cttatcgatt agttagaaat ggcaaaataa
    20221 taataatctc agcaacgatt ttcaataatg ttaaaatttg tttcttcata tttctccact
    20281 cctcattagt agtaacttta ttataccaga gaagtaatca aattcaccaa aatcgcaaat
    20341 ctgaaatttc tataagaaaa aatatcaaat atgcgatttt ctaaaataag ccaattttcg
    20401 tgttatactg tacttgtaaa gcacttgaag caaatcctag gtcgcagaag tggtttacaa
    20461 atgaagataa ttgaaggagt gtaaaagatg ttaaatttac aatttgcaga aacaatggaa
    20521 ttgacagaag ctgagttgga gatagtttat ggaggggaat ttgggaataa tgctgttatc
    20581 ccagccggtg cttggggagg gttcgggaca ccttggtcaa tcactaattt ctggaagaaa
    20641 aattttaacg atagacctga ctttgattct gaccgccgtc gttattaata tccaaagccc
    20701 ttgccttttc ttgggtaggg gctgtcttta tttcatagta taggaggaaa tagaatgaaa
    20761 aagataatct cacaccgcta ctttattata gtttttctac tagttattgc tgaccagaag
    20821 ttcagtgttc tagttttacg cagcgacctt gttactgggc tatctgactt tgcctattat
    20881 ctgtcggata tgatgttgaa ttttcttgtg gttttatttg ctcttattgc tatgatttgg
    20941 tcggggaaat ggcagaaaat caatagtaga aagtttaaag gatcctatct tttctattcc
    21001 ttcctagctc tgcttgcttt tgttgtttgg aatttcgtta ccttttttct tttcccacct
    21061 actcgaaatg aaatttctta tcaacatgct gctccgactt ttacaggggc tacagcattt
    21121 ttgatgtatt ttttctatcc tgtgattgca ggtcccattt ttgaagagat aatccatcgg
    21181 ggattggtga tgacagctct ggaaaaagga aagaaatggg ggctagatgt acttggctct
    21241 gctattttat ttggggtctc gcacattagt aatcacggtt gggtcttgac agactttgtc
    21301 ttctatatgg gtggaggtct tatatttgca gtcttattta gaatgacaaa gtcaatttat
    21361 tggcctattg gactgcatat agtctacaat ggtattggtc agcttttgat gttactgtaa
    21421 ttttggttgt tagtagtatc gcggtctatt cttggggaaa gattcccatt tgaacttaag
    21481 aaggagttgt aatgaaagta tttcttcaaa atagagattt taggcaatta actatcaacc
    21541 agtggatttc aacgcttggg gatacgattt tttatctggc cttcctgaat tatgtggcag
    21601 atgcgtcttt tgcccctttg gcgattttac ttatcacgat ttcagaaacc cttccccaag
    21661 ttctacaaat ctttctggga gttttggcgg attttcaaca tcatcgtgtc ttgaaataca
    21721 cagtcattag ttttacaaaa tttttgcttt actctatagt gtccctatcg ctttcagggc
    21781 agtctttttc cttgttgcta gtaaccttta tttgtatgat taacctcttg tctgacacat
    21841 tgagttattt ttcaggcgcc atgctcactc cgattttcat tagaattatt gggcaagatc
    21901 atctggcaga agctattgga tttaaacagt caactgttag tttagtgaaa acaatcagta
    21961 atatcctagg aggagtctta ctaggtattc tatccatcca gtttatttcc ttactgaacg
    22021 ctcttacctt tttaatcgca tttttaggta tcctcttcat aaaaactgac ctcttgaaag
    22081 tagaaaaaac gattagctat caagaaggac tctctgtaaa atccttttgc cagcatttgc
    22141 tccaatcatc gaaattgata tggaatatga ataaggtgct cttggttttg tttattatct
    22201 ctactagtca agcagtgata aatgttacag ttcctgtctc tactctattt ttgaggaatc
    22261 aacccttttt gaatttacaa acaggtcaat ctcttgcctt gctatccact tttgaactgt
    22321 cagccctgat tgtcggaagc cttgtaagtg gctatctgca acataccatt tccataaaaa
    22381 cagctctcta tgcctcactt gtcatccagt tgcttcttct agtaggattt gccacagttc
    22441 gttttgactg gattctcatc tttagtacct tggatgcctt tttcgcaggt gttctctctc
    22501 ctagattgca agaactcgtc tttaaacaaa tacctgagga gtcaatggga gcagttcaat
    22561 cttctatcag tgccattacg gttgttttac ctagcttatt tacaatatct ttggtaacca
    22621 ttgctactag ctttggaact ctggcagtta gctttgtttt attgctattt cttctagttg
    22681 cctttgttat gcttctgaat attcgtgaaa gtatttagtg gagaatttct ataatatatg
    22741 tttgagactg gattgccagt ctttttatga tggtcaaaat caacagactt tttctctttt
    22801 ctgaatgtg
//
LOCUS       DBERYS010000033        22633 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.033, whole genome shotgun sequence.
ACCESSION   DBERYS010000033 DBERYS010000000
VERSION     DBERYS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.033"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..223
                     /locus_tag="ACL8LA_001425"
     CDS             <1..223
                     /locus_tag="ACL8LA_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813173.1"
                     /translation="AEDKAYNHALSKDRSKVENILAKVKTFKMFSTTYRNHRKRFGYF
                     RHTIYFLFNYSLLYSKGCGCLCPSTHYDI"
     gene            174..908
                     /locus_tag="ACL8LA_001426"
     CDS             174..908
                     /locus_tag="ACL8LA_001426"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGS1813174.1"
                     /translation="MQKVAGASVRVPTMIFNLLNIYKFPLGVSWYLYTLWSILIVYGL
                     LSVVFKNRKSLLLVSVFAYIFTLFIQTDIFIVQRTLVWGICFFLGSVLSEIHFDKINL
                     KKFLFFFVLFDFIYMFAWFLFYEVGSKKDYVSYINPGLWGIAFIVCVLVAFAIFPKME
                     KNFPKTFLYFTKYGKDSLGIYILHAPICSMIRILMLKVGINSVFLHVVVGIVLGWYLS
                     ILATYILKKIPFLNIVLLPQKYIKLK"
     gene            1086..3068
                     /locus_tag="ACL8LA_001427"
     CDS             1086..3068
                     /locus_tag="ACL8LA_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1813175.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGGIKDFSQVG
                     IKVLDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            3369..8672
                     /locus_tag="ACL8LA_001428"
     CDS             3369..8672
                     /locus_tag="ACL8LA_001428"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGS1813176.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDERDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KQALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEITVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASVTVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(8839..10998)
                     /gene="pbp1a"
                     /locus_tag="ACL8LA_001429"
     CDS             complement(8839..10998)
                     /gene="pbp1a"
                     /locus_tag="ACL8LA_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGS1813177.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10995..11591)
                     /gene="recU"
                     /locus_tag="ACL8LA_001430"
     CDS             complement(10995..11591)
                     /gene="recU"
                     /locus_tag="ACL8LA_001430"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGS1813178.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            11657..12184
                     /locus_tag="ACL8LA_001431"
     CDS             11657..12184
                     /locus_tag="ACL8LA_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGS1813179.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            12254..12583
                     /gene="gpsB"
                     /locus_tag="ACL8LA_001432"
     CDS             12254..12583
                     /gene="gpsB"
                     /locus_tag="ACL8LA_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGS1813180.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            12604..12984
                     /gene="rnpB"
                     /locus_tag="ACL8LA_001433"
     ncRNA           12604..12984
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACL8LA_001433"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13069..14226
                     /locus_tag="ACL8LA_001434"
     CDS             13069..14226
                     /locus_tag="ACL8LA_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGS1813181.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREMRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            14239..15633
                     /gene="mapZ"
                     /locus_tag="ACL8LA_001435"
     CDS             14239..15633
                     /gene="mapZ"
                     /locus_tag="ACL8LA_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGS1813182.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLGKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            15709..17133
                     /gene="gndA"
                     /locus_tag="ACL8LA_001436"
     CDS             15709..17133
                     /gene="gndA"
                     /locus_tag="ACL8LA_001436"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGS1813183.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPYLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            17145..17834
                     /locus_tag="ACL8LA_001437"
     CDS             17145..17834
                     /locus_tag="ACL8LA_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1813184.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGIGYTMQE"
     gene            17932..18954
                     /gene="cbpC"
                     /locus_tag="ACL8LA_001438"
     CDS             17932..18954
                     /gene="cbpC"
                     /locus_tag="ACL8LA_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGS1813185.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            18972..19970
                     /gene="cbpJ"
                     /locus_tag="ACL8LA_001439"
     CDS             18972..19970
                     /gene="cbpJ"
                     /locus_tag="ACL8LA_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HGS1813186.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(20088..21320)
                     /locus_tag="ACL8LA_001440"
     CDS             complement(20088..21320)
                     /locus_tag="ACL8LA_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1813187.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(21363..22238)
                     /locus_tag="ACL8LA_001441"
     CDS             complement(21363..22238)
                     /locus_tag="ACL8LA_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS1813188.1"
                     /translation="MYPPELDSKILSIIKKAVTIRTTHTYEYQYSLLFGDAGYLWLLL
                     HLFSISKNQYYLQLANVTAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLK
                     IFIHNSIGEIYHYFLQRDTAKESILDYSFAHGYCGIAYALFAYSKVLEPSMFYNDLHT
                     FHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLCMYDCDGNKDIISKYQEFVFN
                     HHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLMFQGDSGKAD
                     LFDFGIGSMGYIGVY"
BASE COUNT         7054 a         3985 c         5032 g         6562 t
ORIGIN      
        1 agctgaagat aaagcctata accatgcact gtctaaggat agaagcaagg ttgagaacat
       61 ccttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg
      121 cttcggatat tttaggcata ctatatattt tttattcaat tattcacttt tgtattcaaa
      181 aggttgcggg tgcctctgtc cgagtaccca ctatgatatt taacctattg aatatctata
      241 aatttcctct gggagtttca tggtatctat atacgttatg gtcgatattg atagtttatg
      301 gtttactatc tgttgttttc aaaaatcgta agtccctttt gttagttagt gtttttgcct
      361 atattttcac actatttatt caaactgata tttttattgt gcaacggacg ttggtttggg
      421 ggatttgttt ctttcttggc agtgtattga gtgaaattca ctttgataaa attaatttga
      481 aaaaatttct ttttttcttt gtgttatttg attttattta tatgttcgct tggttcttgt
      541 tttatgaagt agggtctaag aaggattatg taagctatat taacccaggt ttgtggggga
      601 ttgcttttat tgtctgtgta ttagttgctt ttgcgatttt tcctaaaatg gagaaaaatt
      661 ttcctaaaac tttcctatat ttcactaaat acgggaaaga tagtttaggg atctatattc
      721 ttcatgcacc aatttgtagc atgattcgga ttctaatgtt gaaagtggga ataaactcag
      781 tttttcttca cgttgttgtt gggattgtgc taggctggta tttatccata ctagcaactt
      841 atatattgaa aaaaattcca tttttgaata ttgttttatt accacaaaag tatattaaat
      901 taaaataaat tattttagat ttataatgtg gctctttgtc aactaacatc tggagaggac
      961 aatcactgtc ttctcttttt ttgtcttttc agaatatacc aaattaacac aaaaattctg
     1021 aaaattctgt tgacatcttt ctgaaaagag tttataatgg agagaaagtt ttaaaggaga
     1081 aaatgatgaa aagttcaaaa ctatttgccc ttgcgggcgt gacattattg gcggcgacta
     1141 ctttagctgc atgctctgga tcaggttcaa gcactaaagg tgagaagaca ttctcataca
     1201 tttatgagac agaccctgat aacctcaact atttgacaac tgctaaggct gcgacagcaa
     1261 atattaccag taacgtggtt gatggtttgc tagaaaatga tcgctacggg aactttgtgc
     1321 cgtctatggc tgaggattgg tctgtatcca aggatggatt gacttacact tatactatcc
     1381 gtaaggatgc aaaatggtat acttctgaag gggaagaata cgcggcagtc aaagctcaag
     1441 actttgtaac aggactaaaa tatgctgctg ataaaaaatc agatgctctt taccttgttc
     1501 aagaatcaat caaagggttg gatgcctatg taaaaggggg aatcaaagat ttctcacaag
     1561 taggaattaa ggttctggat gaacagacag ttcagtacac tttgaacaaa ccagaaagct
     1621 tctggaattc taagacaacc atgggtgtgc ttgcgccagt taatgaagag tttttgaatt
     1681 caaaagggga tgattttgcc aaagctacgg atccaagtag tctcttgtat aacggtcctt
     1741 atttgttgaa atccattgtg accaaatctt ctgttgaatt tgcgaaaaat ccgaactact
     1801 gggataagga caatgtgcat attgacaaag ttaaattgtc attctgggat ggtcaagata
     1861 ccagcaaacc tgcagaaaac tttaaagatg gtagccttac agcagctcgt ctctatccaa
     1921 caagtgcaag tttcgcagag cttgagaaga gtatgaagga caatattgtc tatactcaac
     1981 aagactctat tacgtatcta gttggtacaa atattgaccg tcagtcctat aaatacacat
     2041 ctaagaccag cgacgaacaa aaggcatcga ctaaaaaggc tctcttaaac aaggatttcc
     2101 gtcaggctat tgcctttggt tttgatcgta cagcctatgc ctctcagttg aatggacaaa
     2161 ctggagcaag taaaatcttg cgtaatctct ttgtgccacc aacatttgtt caagcagatg
     2221 gtaaaaactt tggcgatatg gtcaaagaga aattggtcac ttatggggat gaatggaagg
     2281 atgttaatct tgcagattct caggatggtc tttacaatcc agaaaaagcc aaggctgaat
     2341 ttgctaaagc taaatcagcc ttacaagcag aaggtgtgac attcccaatt catttggata
     2401 tgccagttga ccagacagca actacaaaag ttcagcgcgt ccaatctatg aaacaatcct
     2461 tggaagcaac tttaggagct gataatgtca ttattgatat ccaacaacta caaaaagacg
     2521 aagtaaacaa tattacatat tttgctgaaa atgctgctgg cgaagactgg gatttatcag
     2581 ataatgtcgg ttggggtcca gactttgccg atccatcaac ttaccttgat atcatcaaac
     2641 catctgtagg agaaagtact aaaacatatt tagggtttga ctcaggggaa gataatgtag
     2701 ctgctaaaaa agtaggtcta tatgactacg aaaaattggt tactgaggct ggtgatgagg
     2761 ctacagatgt tgctaaacgc tatgataaat acgctgcagc ccaagcttgg ttgacagata
     2821 gtgctttgat tattccaact acatctcgta cagggcgccc aatcttgtct aagatggtac
     2881 catttacaat accatttgca ttgtcaggaa ataaaggtac aagtgaacca atcttatata
     2941 aatacttgga acttcaagac aaggcagtca ctgtagatga ataccaaaaa gctcaggaaa
     3001 aatggatgaa agaaaaagaa gagtctaata aaaaggctca agaagatctc gcaaaacatg
     3061 tgaaataact gttgcaaaat ataagaaagg atttagtatt tctcttgaat gctgaatcct
     3121 tttttacatt tgtaaagaaa gattctaaat gtactgaccc ccaaaagttg gagcctcttt
     3181 ttgtcagaat agagaaaatt tttgttaatt ttacttgttt cctattgctt tatcagttat
     3241 tatttgttat attaaaagta taattatttt ttatttatca gggttaagca ttgcactttc
     3301 agaggaagga gtatttttta aaaaggaaat gtaaacgctt gcccaaaaat gaaaggattt
     3361 agaagtttat gaataaagga ttatttgaaa aacgttgtaa atatagtatt cggaaatttt
     3421 cattaggtgt tgcttctgtt atgattggag ctgcattctt tgggacaagt ccggttcttg
     3481 cagatagcgt gcagtctggt tctacggcga atttaccagc tgatttagct actgctcttg
     3541 caacagcaaa agagaatgat gagcgtgatt ttgaagcgcc taaggtggga gaagaccaag
     3601 gttctccaga agttacagat ggacctaaga cagaagaaga actattagca cttgaaaaag
     3661 aaaaaccggc tgaagaaaaa ccaaaagagg ataaacctgc agctgctaaa cctgaaacac
     3721 ctaagacggt aacccctgaa tggcaaacgg tagagaaaaa agaacaacaa ggaacagtca
     3781 ctatccgaga agaaaaaggt gtccgctaca accaactatc ctcaactgct caaaatgata
     3841 acgcaggcaa acaagccctg tttgaaaaga agggcttgac cgttgatgcc aatggaaatg
     3901 caactgttga tttaaccttc aaagaagatt ctgaaaaggg caaatcacgc tttggtgtct
     3961 tcttgaaatt taaagatacc aataataatg tttttgtcgg ttatgacaag gatggctggt
     4021 tctgggagta taaatctcca acaactagca cttggtatag aggtagtcgt gttgctgctc
     4081 ctgaaacagg atcaacaaac cgtctctcta tcactctcaa gtcagacggt cagctaaatg
     4141 ccagcaataa cgatgtcaat ctctttgaca cagtgactct accagctgcg gtcaatgacc
     4201 atcttaaaaa tgagaagaag attcttctca aggcgggctc ttatgacgat gagcgaacag
     4261 ttgttagcgt taaaacggat aaccaagagg gggtaaaaac agaggatacc cctgctgaaa
     4321 aagaaacagg tcctgaagtt gatgatagca aggtgactta tgacacgatt cagtctaagg
     4381 tcctcaaagc agtgattgac caagccttcc ctcgtgtcaa ggaatacagc ttgaatggac
     4441 atactttgcc aggacaggtg caacagttca accaagtctt tatcaataac caccgaatca
     4501 cccctgaagt cacttataag aaaatcaatg agacaacagc agagtacttg atgaagattc
     4561 gcgatgatgc tcacttaatc aatgcggaaa taacagtacg cttgcaagtt gtggacaatc
     4621 aattgcactt tgatgtgacc aagattgtca accacaatca agtcactcca ggtcaaaaga
     4681 ttgatgacga aagaaaacta ctttcttcta ttagtttcct cggcaatgct ttagtctctg
     4741 tttctagtga tcaaactggt gctaagtttg atggggcaac catgtcaaac aatacgcatg
     4801 tcagcggaga tgatcatatc gatgtaacca atccaatgaa ggatttggct aagggttaca
     4861 tgtatggatt tgtttctaca gataagcttg ctgctggtgt ttggagtaac tctcaaaata
     4921 gctatggtgg tggttcgaat gactggactc gtttgacagc ttataaagaa acagtcggaa
     4981 atgccaacta tgtaggaatc catagctctg aatggcaatg ggaaaaagct tataagggca
     5041 ttgttttccc agaatacacg aaggaacttc caagtgctaa ggttgttatc actgaagatg
     5101 ccaatgcaga caagaaagtt gattggcaag atggtgccat tgcttatcgt agcattatga
     5161 acaatcctca aggttgggaa aaagttaagg atatcacagc ttaccgtatc gcgatgaact
     5221 ttggttctca agcacaaaac ccattcctta tgaccttgga tggtatcaag aaaatcaatc
     5281 tccacacaga tggtcttggg caaggtgttc tccttaaagg atatggtagc gaaggccatg
     5341 actctggtca cttgaactat gctgatattg gtaagcgtat cggtggtgtc gaagacttca
     5401 agaccctaat tgagaaggct aagaaatatg gagctcatct aggtatccac gttaacgctt
     5461 cagaaactta tcctgagtct aaatacttca atgaaaaaat tctccgtaag aatccagatg
     5521 gaagctatag ctatggttgg aactggctag atcaaggtat caacattgat gctgcctatg
     5581 acctagctca tggtcgtttg gcacgttggg aagatttgaa gaaaaaactt ggtgacggtc
     5641 tcgactttat ctatgtggac gtttggggta atggtcaatc aggtgataac ggtgcctggg
     5701 ctacccacgt tcttgctaaa gaaattaaca aacaaggctg gcgctttgcg atcgagtggg
     5761 gccatggtgg tgagtacgac tctaccttcc atcactgggc agctgacttg acctacggtg
     5821 gctacaccaa taaaggtatc aacagtgcca tcacccgctt tatccgtaac caccaaaaag
     5881 atgcttgggt tggggactac agaagttatg gtggtgcagc caactatcca ctgctaggtg
     5941 gctacagcat gaaagacttt gaaggctggc aaggaagaag tgactacaat ggctatgtaa
     6001 ccaacttatt tgcccatgac gtcatgacca agtatttcca acacttcact gtaagcaaat
     6061 gggaaaatgg tacaccggtg actatgaccg ataacggtag cacctataaa tggactccag
     6121 aaatgcgagt ggaattggta gatgctgaca ataataaagt agttgtaact cgtaagtcaa
     6181 atgatgtcaa tagtccacaa tatcgcgaac gtacagtaac gctcaacgga cgtgtcatcc
     6241 aagatggttc agcttacttg actccttgga actgggatgc aaatggtaag aaactttcta
     6301 ctgataagga aaagatgtac tacttcaata cgcaggccgg tgcaacaact tggacccttc
     6361 caagcgattg ggcaaagagc aaggtttacc tttacaagct aactgaccaa ggtaagacag
     6421 aagagcaaga actaactgta aaagatggta aaattactct agatcttcta gcaaatcaac
     6481 catacgttct ctatcgttcg aaacaaacca atcctgaaat gtcatggagc gaaggcatgc
     6541 acatctatga ccaaggattt aacagtggta ccttgaaaca ttggaccatt tcaggcgatg
     6601 cttctaaggc agaaattgtc aagtctcaag gggcaaacga tatgcttcgt attcaaggaa
     6661 acaaagaaaa agttagtctc actcagaaat taactggctt gaaaccaaat accaagtatg
     6721 ccgtttatgt cggtgtcgat aaccgtagta atgccaaggc gagcgtcact gtaaatactg
     6781 gtgaaaaaga agtgactact tataccaata agtctctcgc cctcaactat gtaaaagcct
     6841 acgcccacaa tacacgtcgt aacaatgcta cagttgacga tacaagttac ttccaaaaca
     6901 tgtacgcctt ctttacaact ggatcggacg tatcaaatgt tactctgaca ttgagtcgtg
     6961 aagctggtga tcaagcaact tactttgatg aaattcgtac ctttgaaaac aattcaagca
     7021 tgtacggaga caagcatgat acaggtaaag gcaccttcaa gcaagacttt gaaaatgttg
     7081 ctcagggtat cttcccattt gtagtgggtg gtgtcgaagg tgttgaagat aaccgcactc
     7141 acttgtctga aaaacacaat ccatatacac aacgtggttg gaatggtaag aaagtcgatg
     7201 atgttatcga aggaaattgg tcactcaaga caaatggact agtgagccgt cgtaacttgg
     7261 tttaccaaac catcccacaa aacttccgtt ttgaagcagg taagacctac cgtgtaacct
     7321 ttgaatacga agcaggatca gacaatacct atgcttttgt agtcggtaag ggagaattcc
     7381 agtcaggtcg tcgtggtact caagcaagca acttggaaat gcatgaattg ccaaatactt
     7441 ggacagattc taagaaagcc aagaaggcaa ccttccttgt gacaggtgca gaaacaggcg
     7501 atacttgggt aggtatctac tcaactggaa atgcaagtaa tactcgtggt gattctggtg
     7561 gaaatgccaa cttccgtggt tataacgact tcatgatgga taatcttcaa atcgaagaaa
     7621 ttaccctaac aggtaagatg ttgacagaaa atgctctgaa gaactacttg ccaacggttg
     7681 ccatgactaa ctacaccaaa gagtctatgg atgctttgaa agaggcggtc tttaacctca
     7741 gtcaggccga tgatgatatc agtgtggaag aagcgcgtgc agagattgcc aagattgaag
     7801 ctttgaagaa tgctttggtt cagaagaaaa cggctttggt agcagatgac tttgcaagtc
     7861 ttacagctcc tgctcaggct caagaaggtc ttgcaaatgc ctttgatggc aatgtgtcta
     7921 gtctatggca tacatcttgg aatggtggag atgtaggcaa gcctgcaacc atggtcttga
     7981 aagaaccaac tgaaatcact ggacttcgtt atgttccacg tggatcaggt tcaaatggta
     8041 acttgcgtga tgtgaaactt gttgtgacag atgagtctgg caaggagcat acctttactg
     8101 caactgattg gccagataac aataagccaa aagacattga ttttggtaag acaattaagg
     8161 ctaagaaaat tgtccttaca ggtactaaga cttacggaga tggtggcgat aaataccaat
     8221 ctgcagcgga acttatcttt actcgtccac aggtagcaga aacacctctt gacttgtcag
     8281 gctatgaagc agctttggct aaggctcaga aattaacaga caaagacaat caagaggaag
     8341 tagctagcgt tcaggcaagc atgaaatatg cgacggataa ccatctcttg acggaaagaa
     8401 tggtggaata ctttgcagat tatctcaacc aattaaaaga ttctgctacg aaaccagatg
     8461 ctccaactgt agagaaacct gagtttaaac ttagctcttt agtttccaat caaggtaaga
     8521 cgccagatta taagcaagaa atagatagac cagaaacacc tgaacaaatc ttgccagcaa
     8581 caggtgagag tcaatctgac acagccctct tcctagcaag tgttagtcta gccctatctg
     8641 ctctctttgt agtaaaaacg aagaaagact agtatttagt aaaacctctt aacaagatta
     8701 cggaagcagt ctctatcttt tccaatgagg tttatagtac agaaaaagcc tgagaagatg
     8761 tcttctcagg cttttgttaa gcacataaat acaatagtgc tatgacaaaa tcacccagaa
     8821 aaatctgggt gataaatgtt atggttgtgc tggttgagga ttctgatttt gttgatcagg
     8881 ggttgtattt gattgttgcg tattattgtt aggattggta gtcgtactat tatttgtgct
     8941 tggagtggtt gagctagact gtgaagttga actatctgat gatgagcttg aactttcagt
     9001 tgatgggggt tgttgtggag caggtgagtt ccacgtagaa cgagcaccat ttttaaatac
     9061 gaattctcca tttctgtaga gcccctctgg tatattccaa tcctctggat tgcttccttc
     9121 agacagatag gtcatcatag agcggtaaac tttggcagcg accgtaaggc cattgcctac
     9181 aagtggtgtc agacggttag aatagcctgt ccatacagcc attgaatatt tacgcgtata
     9241 gccagcaaat agttcatcag gtgctacaaa ttgagaggtc ttgatgtggt tttcaatttc
     9301 ctcgtctgta tagttagagg ttcctgtttt accagcctga gggagccaag caagataggc
     9361 atttcgtcca gttccataac tcaagactgt tttcatcatg tcggtcatca tataggctgt
     9421 cgtttccttc atggcacgag ttccgacatt agagaactct ttttcactcc catcactaaa
     9481 gacgacttta tggatataca ttggtttata gtaagttcca ccatttgcaa aggcagcgta
     9541 agcagcagcc atcttttcac tacttgctcc atattttttg tctgattcgg ttgtgttact
     9601 tgaaatggca tttgagtagt gaatacttgg gtagtcgatt ccgagaccat ttaggaaagt
     9661 cttggcgcgg ttgagtccga ccttgtttag agtttccacg gctgggacgt ttcgcgattg
     9721 ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc ctatcccagt tataaacagg
     9781 ggtatttgtc ccagggtagt tatagggctc atcgtgaacg atagtagcag ttgaatcgta
     9841 gataccgtac tccaaggcag gagcatagtc tgtgatcggt ttcatagttg atccccagtc
     9901 gcggtttgtt tctactgctt ggttaattcc gaaggaaaca ttacttgact gatggcgtgc
     9961 tcctagctgg gcaatgactt taccgttaga aacatcaaca atggtagaag cgacttgcaa
    10021 ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa atatcccaca gatgtttttg
    10081 agcttcttgg tctacatttg tgtagacatc catcccagtt gtgagcaggt tatagcctgt
    10141 ttcttcttca acttgattga tgacttcctt gaggtaatta tccatgtaag cagggtaatt
    10201 acttgctgat ttgagacttt gtagtccatc agtaattggt gtattgactg ctttctcata
    10261 ctgttcagca gagatgtagc cttgattttt catttcagat aagaccaagt ttcggcggtc
    10321 ttgggctgct tctggatgtg aataggggtc atattggttt ggtgcctgag gcattccagc
    10381 cagcaaggct aactgaggta aacttaaatt attgaggtct ttaccatagt agttttgagc
    10441 tgctgtctgc attccatagt tcccattaga catgtagacc ttatttatat agtaggtcaa
    10501 gatttcttgc ttggttgctt tttgttctaa ctgaatcgct aaccaagctt cctgagcctt
    10561 acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa gtcaacttaa tcaactgttg
    10621 ggtgagagtt gatccacctt ggagggaatt gctttgcaga ttgcgcaaga aagctcctag
    10681 gatacggatg gtatcaatcc ccctgtggtc gaagaagcga tggtcttcga tagaaacgat
    10741 tgccttaacc aaatctgtgg gaatatcatt agcttgggca ttgacgcggc gttcagaacc
    10801 caagtcagca atgagttgat ttttattgtc gtagatttta ctagaagttg ttgcaactag
    10861 tttactctcg gataggctag gagccttgct aacgtagtag aaaaaaactc ctccgcctaa
    10921 gacaatggct gcgataacca agcttaagaa gctaatgctc agatacttga ttaggcgcag
    10981 aatcgttggt ttgttcatct tgttttacca cctaataaat gttctttgat aacattgaga
    11041 taaggaattt gagggaaggc accagccttg atttcatatc catattctcg aatatattca
    11101 agtggcattg atttttgtcc cttatcttga tgatagaagc gaatcaaatc gaatgccggc
    11161 aataagtagg tttcttgctg agaagaaaag tgaagaagga caaagcagat tccttgttgg
    11221 gcaaggactt gttccatatg ctgaatctga tgtggatgaa aatttttcat cggaatcgca
    11281 cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat atccattata aacgccagaa
    11341 tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa tcttggcacg acttcgttgt
    11401 ggatagtcca cttgtacgat ttgaatagga gttggtttct tatgtataac agccaagccc
    11461 tgagacaaat agtagtcgtt ggtagcattg atcatctttt caaaagacat tcctcgattt
    11521 gcgaaatttt tgggttgaga aagagatgtt tgtctttttt gtgatgaaac tttatgtgga
    11581 tagttgacca taattctcct tattggtaca ataacatcac tttattatac cataatttgc
    11641 acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc tattctgctt ttgatttggg
    11701 tttattttct gataaggatc ctcgtcttaa attgattaaa aaagcgatac gtaaagattt
    11761 agaggctatg gcagcagatg gggtgtcttg gcttgtgttt acagggagtt tgggttttga
    11821 atattgggtt ttagaggttg ctcaggagat gaaaactgaa tacggtttcc agttggccac
    11881 catttttgct tttgaaaccc atggggaaaa ttggaatgaa ggcaatcaga tgaaactgag
    11941 tcgctttaag caggtagact ttgtcaaata tgcctatcct cgctatgaac acaaggggca
    12001 gttaagagat taccagcagt ttttgctgga aaacacgact agttcctatc ttttttatga
    12061 tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag atgaaaaatc aagaggacta
    12121 ttttataaag agattaacat ttgatgaatt aaatgaactt gctgaaaatt tttccgaaaa
    12181 ttgaagcttt gaccttgatt tttgtttgcc tttttttata taataatact agcaagcgag
    12241 aatggagaga gacatggcaa gtattatttt ttcagcgaaa gatatttttg aacaagagtt
    12301 tggacgtgaa gtccgtggct ataataaagt agaagttgac gagtttttag acgatgtcat
    12361 caaggactat gaaacctatg ctgccttggt caagtcactt cgtcaggaaa ttgcggattt
    12421 gaaggaagaa ttaactcgta aaccgaaacc ttcaccagtt caagcagaac cccttgaagc
    12481 ggcaattaca agttctatga cgaattttga tattttgaaa cgcctgaata gattggaaaa
    12541 agaagttttt ggtaaacaaa ttttagataa ctcagatttt taagtagtta tttgagatgt
    12601 gcaatttttg gataatcgcg tgaggagaat tgcttctcat gaggaaagtc catgctagca
    12661 caggctgtga tgcctgtagt gtttgtgcta ggcgaaacca taagcctagg gacgagaaat
    12721 cgttacggca gttgaaatgg ctaagtcctt ggataggcca gagtaggctt gaaagtgcca
    12781 cagtgacgga gtctttctgg aaacagagag agtggaacgc ggtaaacccc tcaagctagc
    12841 aacccaaatt ttggtcgggg catggagtac gcggaaacga acgtagtatt ctgactgcta
    12901 tcagctagag ctgttagtgg tagacagatg attatcgaag gaagtggtcc tagtcacttc
    12961 tggaacaaaa catggcttat agaaaattgc atataggttg gggctgagaa attttctcaa
    13021 cctcattttt taaagtggac atatagaaag gtcttgcaag actgtaacat gaaaaaagaa
    13081 tttaatttaa ttgcaactgt ggcagcaggg cttgaggctg tcgttggtcg tgaaatgcga
    13141 gagttgggct acgattgtca ggttgaaaat ggacgtgttc gttttcaagg agacgtgaga
    13201 gctattatcg aaaccaacct ttggcttcgg gcagcagatc gtatcaaaat tatcgtagga
    13261 acgttcccag ctaagacttt tgaagagcta tttcagggag ttttcgcttt agattgggaa
    13321 aattatttac cacttggagc tcggttcccg atttcaaaag ctaaatgtgt taagtccaaa
    13381 cttcacaatg agcccagtgt tcaggctatt tctaagaaag ctgttgtcaa gaaattgcag
    13441 aaacactatg ctcgcccaga aggggttcct ctgatggaga atggtccaga gtttaagatt
    13501 gaggtctcta ttctcaaaga tgtggcaact gtcatgattg atacgaccgg gtctagcctc
    13561 tttaaacgtg gttatcgtac cgaaaaaggt ggcgctccta tcaaggaaaa tatggcagca
    13621 gccattttac aactttctaa ctggtatcca gacaagcctt tgattgatcc gacctgtggt
    13681 tcggggactt tctgtattga ggcagttatg attgctagaa agatggcgcc aggtcttcgt
    13741 cgttcttttg catttgagga atggaactgg atcagcgatc gcttgattca agaagtgcgc
    13801 acagaagcgg ctaaaaaagt agaccgtgag cttgagctgg atatcatggg ctgtgatatt
    13861 gatgctcgca tggtggaaat tgctaaggcc aatgctcagg tagctggtgt tgcaggagac
    13921 attactttta agcagatgcg cgtgcaggat ttatgttccg ataaaatcaa tggagtaatc
    13981 atttccaatc cgccttatgg tgaacgtttg tcagatgatg caggggtgac caagctctat
    14041 gctgagatgg ggcaagtatt tgcaccgctg aaaacttgga gcaaatttat cctgactagt
    14101 gatgaagctt ttgaaagcaa gtatggtagc caagcagata agaagcgtaa gttatacaac
    14161 ggaaccttga aagtggatct atatcaatat tttggtcagc gtgtcaaacg gcaagaggta
    14221 aaatagaaag ggatactcat gagtaaaaaa agacgaaatc gtcataaaaa agaaggtcaa
    14281 gaaccgcaat ttgattttga tgaagcaaaa gagctaacag ttggtcaagc tattcgtaaa
    14341 aatgaagaag tggaagcagg agtcttgcct gaggattcca ttttggacaa gtatgttaag
    14401 caacacagag atgaaattga ggcggataag tttgcgactc gtcaatacaa aaaagaggag
    14461 ttcgttgaaa ctcagagtct ggatgattta attcaagaga tgcgtgaggc tgtagagaag
    14521 tcagaagctt cttcggagga agttccatct tctgaagaca tcttactacc cttgcctctg
    14581 gacgatgagg agcaaggctt ggatcctcta ttgctagatg atgaaaatcc aacagaaatg
    14641 actgaagaag tggaagagga gcaaaacctt tctcgtctgg atcaagagga ctcagaaaag
    14701 aaaagtaaaa aaggctttat tttgaccgtt ttggcgcttg tatcagtaat tatttgtgtc
    14761 agtgcttatt atgtctaccg tcaagtggct cgttcgacta aggaaattga aacttctcaa
    14821 tcaactacag ccaatcaatc ggatgtggat gattttaata cactttatga cgccttttac
    14881 acagatagca ataaaacggc tttgaaaaat agccagtttg ataaactgag tcaactcaag
    14941 actttacttg ataagctgga aggtagtcgt gaacatacgc ttgccaaatc taaatatgat
    15001 agtctagtaa cgcaaattaa ggccattcaa gatgtcaatg cacaatttga aaaaccagct
    15061 attgtggatg gtgtgttgga taccaatgcc aaagccaaat cggatgctaa atttacagat
    15121 attaaaactg ggaatacgga gcttgataaa gtgctaggta aggctatcag tcttggtaag
    15181 agccagcaaa caagtacttc tagctcaagt tcaagtcaaa ctagcagctc aagctctagt
    15241 caagcaagct caaatacgac tagtgagcca aaaccaagta gttcaaatga gactagaagt
    15301 agtcgcagtg aagtcaatat gggtctctcg agtgcagggg ttgctgttca aagaagtgcc
    15361 agtcgtgttg cctataatca gtctgctatt gatgatagta ataactctgc ctgggatttt
    15421 gcggatggtg tcttggaaca aattctagca acttcacgtt cacgtggcta tatcactgga
    15481 gaccaatata tccttgaacg tgtcaatatc gttaacggca atggttatta caacctctac
    15541 aagccagatg gaacctatct ctttaccctt aactgtaaga caggctactt tgtcggaaat
    15601 ggcgctggtc atgcggatga cttggattac taagcagtcg ttacaaaatt ctttcttttc
    15661 aaaagtaaaa atgataaaat aaaacaaatg aaacaagagg agtgtcaaat gacaaaagct
    15721 aactttggtg ttgtaggtat ggccgtaatg ggtcgtaacc ttgcccttaa tattgaatca
    15781 cgtggttaca cagttgctat ctacaaccgt agtaaagaaa aaacggaaga tgtgattgct
    15841 tgccatcctg aaaagaactt tgtaccaagc tatgacgttg aaagttttgt aaactcaatc
    15901 gaaaaacctc gtcgtatcat gctgatggtt caagctggac ctggtacaga tgctactatc
    15961 caagcccttc ttccatacct tgacaagggt gatatcttga ttgacggtgg aaatactttc
    16021 tacaaagata ccatccgtcg taatgaagaa ttggcaaact caggtatcaa ctttatcggt
    16081 actggggttt ctggtggtga aaaaggtgcc cttgaaggtc cttctatcat gcctggtgga
    16141 caaaaagagg cctacgaatt ggttgcggat gttcttgaag aaatctcagc taaagcacca
    16201 gaagatggca aaccatgtgt gacttacatc ggccctgatg gagctggtca ctatgtgaaa
    16261 atggttcaca atggtattga gtacggtgat atgcaattga tcgcagaaag ctatgacttg
    16321 atgcaacact tgctaggcct ttctgcagag gatatggctg aaatctttac tgagtggaac
    16381 aagggtgaat tagacagcta tttgatcgaa atcacagctg atattttgag ccgtaaagac
    16441 gatgaaggcc aagatggacc aatcgtagac tacatccttg atgctgcagg taacaaggga
    16501 actggtaaat ggactagcca atcatctctt gaccttggtg taccattgtc actgattact
    16561 gagtcagtgt ttgcacgcta catttcaact tacaaagaag aacgtgtaca tgctagcaag
    16621 gtgcttccaa aaccagctgc cttcaacttt gaaggagaca aggctgaatt gattgaaaag
    16681 atccgtcaag ccctttactt ttcaaaaatc atttcatacg cacaaggatt tgctcaatta
    16741 cgtgtagcct ctaaagaaaa caactggaac ttgccatttg cagatatcgc atctatctgg
    16801 cgtgatggct gtatcatccg ttctcgtttc ttgcaaaaga ttacagatgc ttacaaccgt
    16861 gatgcagacc ttgccaacct tcttttggat gaatacttct tggatgttac tgctaagtat
    16921 caacaagcag tgcgtgatat cgtagctctt gcggttcaag ctggtgtgcc agtgccaact
    16981 ttctcagcag ctattactta ctttgatagc taccgttcag ctgaccttcc agctaacttg
    17041 atccaagcac aacgtgacta ctttggtgct cacacttacc aacgtaaaga caaagaagga
    17101 accttccact actcttggta tgacgaaaaa taagtaggtc agccatgggg aaacggattt
    17161 tattacttga gaaagaacga aatctagctc attttttaag tttggaactc cagaaagagc
    17221 agtatcgggt tgatctggta gaggaggggc aaaaagccct ctccatggct cttcagacag
    17281 actatgattt gatgttattg aacgttaatc tgggagatat gatggctcag gattttgcag
    17341 aaaaattgag ccgaactaaa cctgcctcag tcatcatgat tttagatcat tgggaagact
    17401 tgcaagaaga gctggaagtt gttcagcgtt ttgcagtttc atacatctat aagccagtcc
    17461 ttatcgaaaa tctggtagcg cgtatttcgg cgatcttccg aggtcgggac ttcattgatc
    17521 aacactgcag tctgatgaaa gttccaagga cctaccgcaa tcttaggata gatgttgaac
    17581 atcacacggt ttatcgtggt gaagagatga ttgctctgac ccgccgtgag tatgaccttt
    17641 tggcgacact tatgggaagc aagaaagtat tgactcgtga gcaattgttg gaaagtgttt
    17701 ggaagtatga aagtgcgacc gagacaaata tcgtagatgt ctatatccgc tatctacgga
    17761 gcaagcttga tgttaaagga caaaaaagct acattaaaac tgtgcgtggt attggatata
    17821 ccatgcaaga atagaaaagc agttgcagtt gtgtaactgc ttttttgagg agtttttata
    17881 tattgacatg cagtcgggtc tttgctacaa tcgttttagg aggatatata gatgaaactt
    17941 ttgaaaaaaa tgatgcaagt tctactagca gtctttttct ttggtttgct agctacaaat
    18001 acggtatttg cgaataccac aggtggccga tttgttgata aggataatag aaaatattat
    18061 gtaaaagatg atcataaagc aatctattgg cataaaatag acggtaaaac ttactatttt
    18121 ggtgatattg gagagatggt tgtcggttgg caatacttag aaattcctgg aacaggttat
    18181 cgtgataatt tattcgataa ccaaccagtt aatgaaattg gccttcagga gaagtggtac
    18241 tattttggac aagatggtgc tttgctagaa caaacagata aacaagtact agaggcaaaa
    18301 acgtctgaaa atacaggaaa agtatacggt gaacaatatc ctctatctgc tgaaaagaga
    18361 acttattatt ttgataataa ttatgctgta aagacaggct ggatttatga agagggtcat
    18421 tggtattatt taaataagct aggaaatttt ggcgatgatt cttacaatcc actaccaatt
    18481 ggtgaagttg ctaagggttg gactcaagat tttcatgtta ctattgacat tgatagaagc
    18541 aaacctgctc catggtacta cctagatgct tcaggtaaga tgcttacaga ttggcaaaaa
    18601 gtaaacggaa aatggtatta ttttggctcc tctggttcta tggcaacagg ttggaaatat
    18661 gtacgaggca aatggtatta cttagataat aaaaatggtg atatgaaaac aggatggcaa
    18721 taccttggta acaagtggta ctacctccgt tcatcaggag ctatggtaac tggctggtat
    18781 caagatggtt caacttggta ctatttagat ccttctaatg gagatatgaa aataggttgg
    18841 acaaaagtaa atggaaaatg gtattatctc aattcaaatg gagcaatggt tacaggtagc
    18901 caaactatcg atggtaaagt ttataatttc gcctcatctg gtgagtggat ttaatattgg
    18961 agggtatata gatgaaaatt ttgaaaaaaa ctatgcaagt tggactgaca gtatttttct
    19021 ttggtttgct agggaccagt acagtatttg cagatgattc tgaaggatgg cagtttgtcc
    19081 aagaaaacgg aagaacctac tacaaaaagg gggacctcaa agaaacctac tggcgagtga
    19141 ttgatggtaa gtactattat tttgattctc tatctggaga gatggttgtc ggctggcaat
    19201 atatcccgtt tccatctaaa ggtagtacaa ttggtcctta cccaaatggt atcagattag
    19261 aaggttttcc aaagtcagag tggtactact tcgataaaaa tggagtgcta caagagtttg
    19321 ttggttggaa aacattagag attaaaacta aagacagtgt tggaagaaag tacggggaaa
    19381 aacgtgaaga ttcagaagat aaagaagaga agcgttatta tacgaactat tactttaatc
    19441 aaaatcattc tttagagaca ggttggcttt atgatcagtc taactggtat tatctagcta
    19501 agacggaaat taatggagaa aactaccttg gtggtgaaag acgtgcgggg tggataaacg
    19561 atgattcgac ttggtactac ctagatccaa caactggtat tatgcaaaca ggttggcaat
    19621 atctaggtaa taagtggtac tacctccgtt cctcaggagc aatggccact ggctggtatc
    19681 aggaaggtac cacttggtat tatttagacc acccaaatgg cgatatgaaa acaggttggc
    19741 aaaaccttgg gaacaaatgg tactatctcc gttcatcagg agctatggca actggttggt
    19801 atcaagatgg ttcaacttgg tactacctaa atgcaggtaa tggagacatg aagacaggtt
    19861 ggttccaggt caatggcaac tggtactatg cttatagctc aggtgctttg gcagtgaata
    19921 cgaccgtaga tggctattct gtcaactata atggcgaatg ggttcggtaa tgacagggcg
    19981 cttataatta tattagaaca ctcgcttaat caaagaagtg actttgttgt acttgacctg
    20041 acgattgggg agattaaaag catgtgaaat ctacacatgc tttttcgtta gttatgagtg
    20101 ttgaacttga ctaaccattg acaaaagata agagcaatta aaataagata caaaaatatc
    20161 aatgctaact gcaccgaaac gcccgatgct atggataggg ctaataggga gagcagggac
    20221 ggggcgagaa tgtccactgt gctcagagca gagcccaccg tccccatctt gtcactgggg
    20281 atatgggcaa agacagctgc ttgtaggcga gggctgagaa tgccgataat cgtagaactg
    20341 atgaaactgg tcatgaaaat tacgatatag gcttgacaaa ggatactagt tattagagtc
    20401 aatagggaaa tctcacagaa aaccaagagg tgcgtgaaac ggatattttt aaatagatta
    20461 ctgctggtca tattgccaag gatagctcca caagacatga tgatggagag cagggcgagg
    20521 agttgcccaa tgctcagttg tgctagtgtc tgttggatga agcggagggc aatcagccga
    20581 ggggacacat ccaagatggc aatcatactg gtcgtcagaa acagtagtat gacggtatct
    20641 tttaacctca ggagcacagc cagcgactgt ttaagatgtt gaaaatattt cttaaaactc
    20701 agtgctgtat gtgacatttc aattcttttt tcaacctcat acaaggtatg tcgaataaca
    20761 tacaggccca aaaaggcaat gacaaaagtc agagtgttga taagggaaat agtttgaata
    20821 cttataacat tgataaggaa tgcgccagcc agattggcga caatacggac aaccctcatc
    20881 agagactgcc tgaaccccat ggcatcatgc aggtcgtcct taattacact gatgtagagg
    20941 gcgttcatca tgtaggcaga caggtagctc aaggtgtcag agatgaggtt gatgatgaca
    21001 atcataatga ctgaaactaa tgaaaagggc gacaagacga gaaatactgt caaaatagcg
    21061 tagagcagga ttttgatttt ggcaatccag agtgcgtgtt tgactctatt ttcttgaaaa
    21121 tctcctagaa tcccgagaaa gagttgcgat agtaggggaa ccatctctga aatggaaatc
    21181 agtaaaatcg ctagcggagc gaaattgtac tgagccacat aattgataat ggcgaggtag
    21241 aaaatactgt cgccgaatga tgaaaaacag ctgtttagca gcaactgtct atatatgttg
    21301 tttgtccaaa ataatttcat acttttctcc ttatgtctgc acatcgaatg ggaatttatt
    21361 atttaataga caccaatata ccccatgctt cctattccga agtcaaacaa atctgcttta
    21421 ccactatctc cttgaaacat aagtaaaccg tgatcatctc gttcagaaca tgctaaaatt
    21481 acctgttgga tttttttcat cagtaatttg ttttgattgt agacagtggt ttgtagtaag
    21541 ctagttattc cgtggcaata tcctgtcatc atttttagat gatggttaaa aacaaattct
    21601 tgatatttac taataatatc tttgtttccg tcacaatcgt acatacaaag atataagatt
    21661 attccggaaa ttcctttgca ccaagaaagt tgtaaatttc ctaaattttc agtattagaa
    21721 gtaacttttt ctaataattt ttttaattca gtatggaatg tatggagatc attatagaac
    21781 atagaaggtt ctaagacttt agaataggca aataaagcat atgcaattcc acaatatcca
    21841 tgagcaaagc tatagtctaa aatgctttct ttggctgtat ctctttgtag gaaataatga
    21901 taaatttccc ctatactatt gtggatgaaa attttaagag tattgtcatt ggtaaattga
    21961 tagtatttta ttaatgataa taggacacca gattttccca atgcaaagtc tatttcctct
    22021 agagtatcat aattctctat taatttttta gcggtgacgt ttgctaattg tagatagtat
    22081 tgatttttac tgatagaaaa taaatgtagg agtaaccata gatagcctgc atcaccaaat
    22141 agcagtgagt attgatattc atatgtgtgt gtcgttctaa ttgtcactgc cttcttgatg
    22201 atagagagta ttttagaatc cagttctggg gggtacaatt tatttagagg aaacagtagt
    22261 ccagctattc cgtgttggaa tgataaatta cttacgaaat ttccaaattc tgttgaagaa
    22321 gtgatccttc cttcagaatt tacagtttta tctagtaaaa attgtagaac actattacat
    22381 tctgttttgt aatcatattt atgttcctct atttttttaa ttttgactaa attatttgtt
    22441 tgacttaaat ttagtagaca ttgtaaccaa aatagttgct catctgtgag ctgaccttga
    22501 gtatgtgata gttctagtaa ttgaatagga tcctttagtt agattcaatc aatctataac
    22561 acaacaacat aataatactt ttaggataac ttatcaaata ttatagtaaa atgagataat
    22621 aacaggataa atc
//
LOCUS       DBERYS010000034        21070 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.034, whole genome shotgun sequence.
ACCESSION   DBERYS010000034 DBERYS010000000
VERSION     DBERYS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.034"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            98..2320
                     /gene="recJ"
                     /locus_tag="ACL8LA_001442"
     CDS             98..2320
                     /gene="recJ"
                     /locus_tag="ACL8LA_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGS1813189.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2495..4156
                     /locus_tag="ACL8LA_001443"
     CDS             2495..4156
                     /locus_tag="ACL8LA_001443"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS1813190.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4225..5004
                     /locus_tag="ACL8LA_001444"
     CDS             4225..5004
                     /locus_tag="ACL8LA_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1813191.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5116..6336
                     /gene="murM"
                     /locus_tag="ACL8LA_001445"
     CDS             5116..6336
                     /gene="murM"
                     /locus_tag="ACL8LA_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGS1813192.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6341..7573
                     /gene="murN"
                     /locus_tag="ACL8LA_001446"
     CDS             6341..7573
                     /gene="murN"
                     /locus_tag="ACL8LA_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGS1813193.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEYFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7652..8353
                     /locus_tag="ACL8LA_001447"
     CDS             7652..8353
                     /locus_tag="ACL8LA_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGS1813194.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLAGQ
                     FLSVTVRLNHYYLSNPHYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8393..10240
                     /gene="uvrC"
                     /locus_tag="ACL8LA_001448"
     CDS             8393..10240
                     /gene="uvrC"
                     /locus_tag="ACL8LA_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGS1813195.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGSIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10230..11063
                     /locus_tag="ACL8LA_001449"
                     /pseudo
     CDS             10230..11063
                     /locus_tag="ACL8LA_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(11247..12047)
                     /locus_tag="ACL8LA_001450"
     CDS             complement(11247..12047)
                     /locus_tag="ACL8LA_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1813196.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12218..12823
                     /locus_tag="ACL8LA_001451"
     CDS             12218..12823
                     /locus_tag="ACL8LA_001451"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGS1813197.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12836..14236
                     /gene="pepV"
                     /locus_tag="ACL8LA_001452"
     CDS             12836..14236
                     /gene="pepV"
                     /locus_tag="ACL8LA_001452"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGS1813198.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14290..14868
                     /locus_tag="ACL8LA_001453"
     CDS             14290..14868
                     /locus_tag="ACL8LA_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGS1813199.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15028..15315
                     /locus_tag="ACL8LA_001454"
                     /pseudo
     CDS             15028..15315
                     /locus_tag="ACL8LA_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            15486..16811
                     /gene="brnQ"
                     /locus_tag="ACL8LA_001455"
     CDS             15486..16811
                     /gene="brnQ"
                     /locus_tag="ACL8LA_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGS1813200.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16909..17946
                     /locus_tag="ACL8LA_001456"
     CDS             16909..17946
                     /locus_tag="ACL8LA_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGS1813201.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17948..18409
                     /locus_tag="ACL8LA_001457"
     CDS             17948..18409
                     /locus_tag="ACL8LA_001457"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1813202.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18427..19143
                     /gene="ldcB"
                     /locus_tag="ACL8LA_001458"
     CDS             18427..19143
                     /gene="ldcB"
                     /locus_tag="ACL8LA_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGS1813203.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19266..19691
                     /gene="rplK"
                     /locus_tag="ACL8LA_001459"
     CDS             19266..19691
                     /gene="rplK"
                     /locus_tag="ACL8LA_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGS1813204.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19899..20588
                     /gene="rplA"
                     /locus_tag="ACL8LA_001460"
     CDS             19899..20588
                     /gene="rplA"
                     /locus_tag="ACL8LA_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGS1813205.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6328 a         3883 c         4896 g         5963 t
ORIGIN      
        1 tactatgaaa tgttataaaa aagcaatctg gatagagaaa acgtctaaat catgttataa
       61 tgaagcaata gaattcttag aaggagtgga tgtctttttg ataacaccta cttatgaatg
      121 gcagtttgcc ctgcaggtag aagatgcgga ttttacaaag atagccaaga aggctggact
      181 gggtcctgag gtggctcggt tattgtttga gagagggatt caggaccaag aaagtctgaa
      241 gaagttttta gaaccttcct tggaggactt acatgatgct tatctgctcc atgatatgga
      301 caaggcagtg gagcggattc gtcaggctat tgaagaaagg gaaaatattc tcgtttatgg
      361 agactacgat gcggatggca tgacttcggc ttctattgtg aaggaaagtt tggaacaact
      421 tggtgcggag tgccgagttt acctgccaaa tcgttttacc gatggctatg gccctaatgc
      481 tagtgtttat aaatacttta tcgagcaaga aggaatttcc ttgattgtga cggtggacaa
      541 tggggttgct ggtcatgagg ctattgcatt ggctcagtct atgggagtag atgtcattgt
      601 gacagaccat cattccatgc ctgaaaccct gccagatgct tatgctattg tccatcctga
      661 acatccagat gcggattatc cttttaaata tttggctggt tgtggagttg ctttcaagtt
      721 ggcttgtgcc ctgttagaag aagtgcaagt ggaattgctt gatttggtcg ctattggaac
      781 tattgcagat atggtgagtc tgacggatga aaatcgtatc ttagttcaat atggtctgga
      841 aatgttgggt catacccagc gcattggtct gcaagaaatg ctggacatgg ctgggattgc
      901 tgccaacgaa gtaacagaag aaacggttgg tttccagatt gctcctcgtt tgaatgcctt
      961 gggtcgcttg gatgatccca atcctgccat tgatttgttg actggatttg atgatgagga
     1021 agcgcatgag attgccctta tgattcacca gaaaaacgaa gagcgcaagg aaatcgttca
     1081 gtctatctat gaagaagcca agaccatggt ggatcctgag aagaaggttc aggtcttggc
     1141 caaggaaggc tggaatcctg gggttctagg aatcgtggct ggtcgtttat tggaagaatt
     1201 gggacagaca gtcattgttc ttaatataga agacggtcgt gccaagggca gtgctcgtag
     1261 tgtggaagcg gtcgatattt ttgaagctct ggatccccat cgagacctct tcatcgcctt
     1321 tggaggtcat gcaggtgcag cgggtatgac gctggaagtt gagcaactct cagatttatc
     1381 tcaggttttg gaagattatg ttcgtgaaaa aggtgcagat gctggtggca agaataagtt
     1441 aaacctagat gaagagttgg atttggaggc acttagcttg gaaacggtca aaagttttga
     1501 acgtttagct ccttttggaa tggataatca gaaacctatt ttttatatca agaattttca
     1561 ggtcgaaagt gctcgtacta tgggggcagg taatgcccat ctaaagctga aaatttccaa
     1621 gggtggggcg agttttgaag tggtagcctt tggtcaaggc agatgggcga cagagttttc
     1681 tcaaaccaag aatctagagt tagcggttaa attgtctgtc aaccaatgga atggccaaac
     1741 tgccctccag ttgatgatgg tggatgcgcg agtggaaggt gttcaacttt ttaacattcg
     1801 tggaaaaaat gcagtcttgc cagaaggtgt tccagtcttg gattttcctg gagaactgcc
     1861 aaatcttgcg gctagtgaag ctattgtcgt aaaaaacatt ccagaggata ttactcagct
     1921 gaagaccatt tttcaggaac agcatttctc tgctgtctat ttcaaaaatg atattgacaa
     1981 ggcctattat ctgacaggtt atgggactag agatcagttt gccaaattgt acaagactat
     2041 ttaccagttc ccagagtttg atattcgcta caagctgaaa gatttggctg catatcttaa
     2101 tattcaacaa atcttgctgg tcaagatgat tcaagtattt gaagaactag gctttgtgac
     2161 gataaaagat ggtgtgatga cagtcaataa agaggcgcca aagcgggaga taggagaaag
     2221 tcaaatttac caaaatctca aacaaaccgt taaagaccaa gaaatgatgg cgctgggtac
     2281 ggtgcaagaa atttatgatt ttttgatgga aaaagagtag aagttaggaa agagttggga
     2341 aatcaactct tttttgaaaa cagaccttca ttttgaaaat catcaaaaaa atggtataat
     2401 ggtaggaaaa gattcggctg aaagtattag aacttttaga ataagagggt agagttgccc
     2461 tataatcaag ataaactaag attttggagg aaaaatgagt aatatcagtt taacaacact
     2521 tggtggtgtg cgtgagaatg gaaaaaatat gtacattgct gaaattggag agtccatttt
     2581 tgttttgaat gtagggttaa aatatcctga aaatgaacaa ttaggggtcg atgtggtgat
     2641 tccaaacatg gattaccttt ttgaaaatag cgaccgtatt gctggggttt tcttgaccca
     2701 cgggcatgcg gatgccattg gtgctctacc ttatctcttg gcagaggcta aagttcctgt
     2761 atttgggtct gagttgacca ttgagttggc aaagctcttt gtcaaaggaa atgatgccgt
     2821 taagaaattt aatgatttcc atgtcattga tgagaatacg gagattgatt ttggtgggac
     2881 agtggtttcc ttcttcccta cgacttactc cgttccagag agtctgggaa ttgtcttgaa
     2941 gacatcggaa ggaagcatcg tttatacagg tgacttcaaa tttgaccaaa cggctagtga
     3001 atcttatgca actgattttg ctcgtttggc agagattggt cgtgacggcg tcctggctct
     3061 cctcagtgat tcggccaatg cagacagcaa tattcaggtg gctagtgaaa gtgaagttag
     3121 ggatgaaatt acccaaacta ttgctgactg ggaaggtcgt atcatcgttg cagctgtttc
     3181 cagtaatctt tctcgtattc agcagatttt tgacgctgcg gataaaacag gtcgacgtat
     3241 cgtcttgaca ggatttgata ttgaaaatat cgtccgcaca gcgattcgtc ttaagaagtt
     3301 gtctttagcc aacgaaattc tcttgattaa gcctaaagat atgtctcgct ttgaagacca
     3361 tgagttgatt attcttgaga caggtcgtat gggtgaacct atcaatggac ttcgtaagat
     3421 gtcgattggt cgccatcgtt atgtagaaat caaggatggg gacctggtct atattgctac
     3481 ggctccgtct attgctaaag aagcctttgt tgcgcgtgtg gaaaatatga tttatcaggc
     3541 aggtggggtt gtcaaattga ttacccaaag tttacatgta tcagggcacg gaaatgtgcg
     3601 tgatttgcag ctgatgatca atcttttgca acctaagtac ctcttccctg tccaagggga
     3661 gtatcgtgag ttggatgctc acgctaaggc tgccatggca gttgggatgt tgccagaaca
     3721 catcttcatt cctaaaaagg ggacgaccat ggcttacgag aatggagact ttgttccagc
     3781 tggatcggtt tcagcaggag atatcttgat tgatgggaat gccattggtg atgttggaaa
     3841 tgttgttctt cgtgaccgta aggtcttgtc agaggatgga attttcatcg tggctattac
     3901 agtcaaccgt cgtgagaaga aaattgtggc tagggctcgt gttcatacgc gcggatttgt
     3961 ttatctcaag aagagtcgcg atattctccg tgaaagttca gaattgatta accaaacggt
     4021 agaagagtat cttcaaggag atgactttga ctgggcagat cttaaaggga aggttcgaga
     4081 taatttgacc aagtatctct ttgaccaaac caagcgtcgt ccagctattt taccagtagt
     4141 catggaagca aaataatcgt tgaaataaac agagagaaag tcgagtttcg gctttttctt
     4201 atagaaaaat agaaggagaa aatcatggca gtaatgaaaa tcgagtatta ctcacaagta
     4261 ttggatatgg agtggggggt gaatgtcctc taccctgatg ccaatcgagt ggaagaacca
     4321 gagtgtgaag atattcccgt cttgtacctt ttgcacggga tgtctggaaa tcataatagt
     4381 tggcttaagc ggaccaatgt agaacgcttg cttcgaggaa ctaatctcat cgttgttatg
     4441 cccaatacca gcaatggttg gtacaccgat acccagtatg gttttgacta ctacacggct
     4501 ctagcagagg aattgccaca ggttctgaaa cgcttcttcc ctaatatgac tagcaagcgt
     4561 gaaaagacct ttatcgctgg tctttctatg ggaggctacg gctgcttcaa actggctctt
     4621 gcgacaaatc gtttttctca tgcagctagc ttttcaggtg ccctcagctt tcaaaacttt
     4681 tctcctgaaa gtcaaaatct gggaagtcca gcctactgga gaggtgtttt tggagagatt
     4741 aaagactgga caactagtcc ctattctctt gaaagtctgg ctaaaaaatc ggataaaaag
     4801 accaaacttt gggcgtggtg tggcgaacag gatttcttgt acgaagccaa taatctcgca
     4861 gtgaaaaatc tcaaaaaact aggttttgat gtgacctata gccatagcgc tggaactcac
     4921 gagtggtact actgggaaaa acaattggaa gtttttttaa caaccctacc aattgatttc
     4981 aaattagaag agagactgac ttagtttgaa cttcagcata gggggagtag aactaaaata
     5041 aaatatgttt tcactagact tttcaaacga aagtagtaga atagtaataa aatactggag
     5101 gaaagagagt aggaaatgta ccgttatcaa attggcattc ccacattaga atatgatcag
     5161 tttgtcaaag aacatgaatt agccaatgta ttacaaagta gtgcttggga ggaagttaag
     5221 tctaattggc aacatgagaa gtttggtgtt tacagggaag aaaaattact ggcgacagct
     5281 agtattttga ttagaactct tccgctaggc tataaaatgt tttacatccc aagaggacct
     5341 atattggatt atggggataa agaactcttg aattttgtca ttcagtctat taagtcctat
     5401 gctcgcagta agagagcggt ttttgtgact tttgacccaa gtatttgcct atctcaaagt
     5461 ttaatcaatc aggaaaagac agaatttcct gaaaatctgg ctattattga tagtttgcaa
     5521 caaatgggag taaggtggtc aggaaaaacg gaggaaatgg gagacaccat tcaacctcgt
     5581 attcaggcga aaatatacaa ggaaaatttt gaagaagata aactttccaa gtcaacaaaa
     5641 caggctattc gaacagcacg aaacaaaggg cttgagattc aatatggtgg actggaacta
     5701 ttagattcat tttcggagtt gatgaaaaaa actgagaagc gaaaagagat tcatttgagg
     5761 aatgaagcct attataaaaa attgttagat aattttaagg acaaggccta tatcaccttg
     5821 gccaccttgg atgtttctaa acgttcgcaa gagttagaag aacagttagc gaaaaataga
     5881 gccttggaag agacgtttac tgagtcgact cgaacttcaa aagtagaagc gcagaagaag
     5941 gaaaaagaac gtttgttaga ggaattgacc ttcttgcagg aatatataga tgtaggtcaa
     6001 gcgagagttc ctttagcggc tactttgagt ttggaatttg gtactacctc tgtcaatata
     6061 tatgctggta tggatgatga ttttaaacgt tacaatgcac caattttaac atggtatgaa
     6121 acggctcgct atgcctttga gcgaggtatg gtctggcaaa atttaggtgg tgttgaaaac
     6181 tctctcaatg gtggacttta tcattttaag gaaaaattta atccaacgat tgaagaatac
     6241 ttgggtgaat ttacaatgcc cactcatcct ctctatcctc tgttaagact tgctcttgat
     6301 ttccgtaaaa cattaagaaa aaaacataga aagtaagtat atggcactaa caacactcac
     6361 gaaagaagag tttcagactt attctgatca ggtttcttct cgttccttta tgcaatctgt
     6421 ccagatgggg gatttgctag aaaaaagagg ggctcgaatt gtttatcttg ctttgaaaca
     6481 agaaggagaa attcaagttg cagctctggt ttatagcctg cccatgctgg gtggtctgca
     6541 tatggaactc aattcggggc cgatttatac ccaacaagat gctcttccag ttttttatgc
     6601 agagttaaaa gaatatgcca agcaaaatgg tgtattagag ttgcttgtaa aaccttatga
     6661 aacttatcaa acttttgata gccaaggtaa tccaatagat gctgagaaaa aaagtattat
     6721 tcaaggtttg actgatttag gttatcaatt tgatggctta acaacaggtt acccaggtgg
     6781 agaaccagat tggttatact ataaagattt aactgaatta actgaaaaga gtttgcttaa
     6841 aagttttagc aaaaagggta aacccttggt gaaaaaggct gaaacctttg gcattcggtt
     6901 gaaaaagtta aaacgtgaag aactatcgat ttttaagaat ataacaaaag aaacctctga
     6961 acgtagagaa tatagtgata aaagtttaga atattatgag tatttttatg atacttttgg
     7021 agaacaagcg gagtttctca tagcaagctt gaatttttcg gagtatatga gcaaattgca
     7081 aggtgaacaa agtaaactag aagaaaactt ggacaagttg cgacttgatt tgagtaaaaa
     7141 tcctcattct gagaaaaaac aaaatcaact gagagaatat tctagtcaat ttgaaacgtt
     7201 tgaagttcga aaagcagaag cgcgagactt gattgaaaaa tatggagaag aagatattgt
     7261 tttagctggg agtttatttg tttatatgcc tcaggaaacg acttatctct ttagtggttc
     7321 ctacactgag tttaataagt tctatgcccc tgcactgctt caaaaatatg ttatgttgga
     7381 aagcataaaa cgtggaatac ctaaatacaa tttcctaggc attcaaggga tttttgatgg
     7441 aagtgatggt gttttgcgtt ttaaacagaa ttttaatggc tatattgtac gcaaagcggg
     7501 tactttccgt taccatccat cgcctttaaa atacaaagct atccagttac tcaaaaaaat
     7561 agtaggacgt taagatgaaa aagtcagtat ttagatttct tttagcttct tttagtaaaa
     7621 taattcttat ttgctagaaa ggtggagaga catgcgctgg ctttttcgtt tgataggggc
     7681 tttcttttct tttgtgtggc gtttgttttg gcgtctggtt tggatagttg tgctcttatg
     7741 tgtgcttgct ttcggacttc tctggtatct gaacggggat tttcaaggag cgctaaagca
     7801 agcagaacgg tcagtaaaaa ttggtcaaca aagtattgac caatgggaga aaacagggca
     7861 actgcctaag ttaagccaga cagatagtca ccagcattct gaaggaaggt gggcacaggc
     7921 ctctgctcgt atttacctgg atccgcagat ggattcacgc tttcaagagg cttatttaga
     7981 agcaatccag aactggaatc aaactggtgc ttttaacttt gaactcgtga ctgagtctag
     8041 taaggcggat attacggcta cggagatgaa cgacggaggc actcctgtgg caggagaggc
     8101 ggaaagtcaa actaatctct tagcagggca attcttgtcc gtaacggtgc ggttgaatca
     8161 ttattatttg tccaatccac actatggcta ctcctatgaa cgccttgtcc atacggcaga
     8221 acatgagtta ggtcatgcga ttggcttgga ccatacagat gagaagtctg tcatgcaacc
     8281 agcaggttcc ttttatggta tccaggaaga ggatgttgca aacctccgaa aaatatatga
     8341 gactagtgag tagggtacta tctttcccta ctttttttgc tataatggaa ctatgaacaa
     8401 cttgattaaa tcaaaactag agctcttgcc gaccagccct ggttgctaca ttcataagga
     8461 taaaaatggc tccattatct atgtaggaaa ggctaaaaat ctgcgtaatc gagtacggtc
     8521 ctattttcgt ggaagtcatg ataccaagac agaggctctg gtgtctgaaa ttgtggattt
     8581 tgaatttatt gttacggagt ctaatattga ggcacttctc ctagaaatca acctgatcaa
     8641 ggaaaacaag cccaagtaca atatcatgct caaggatgac aagtcctatc ctttcatcaa
     8701 aatcaccaat gagcgctatc cacgcttgat tatcactcgt caggtcaaaa aggacggagg
     8761 tctttatttt ggaccctatc ccgatgtggg ggcagccaat gaaatcaagc ggttgctgga
     8821 tcggatattc ccttttcgta agtgtaccaa cccgccctct aaggtctgtt tttattacca
     8881 tatcggccag tgtatggctc acaccatctg taagaaggat gaggcttatt tcaagtctat
     8941 ggcccaggag gtttctgatt ttctgaaagg gcaggataac aaaatcattg atgagctaaa
     9001 gggcaaaatg gcagcggcag cccagactat ggagtttgaa cgtgcggcgg aataccgtga
     9061 cctgattcag gctattggaa cgcttcgaac caagcaacgg gtcatggcta aggatctcca
     9121 aaatcgcgat gtctttggct actatgtgga taagggctgg atgtgtgtgc aggttttctt
     9181 tgttcgtcag ggcaagctca tcgagcgcga tgtcaatctc ttcccctact tcaatgatcc
     9241 agatgaggat ttcttgacct atgtgggaca attctatcaa gaaaaatctc acctagttcc
     9301 caatgaggta ctgattccgc aggatattga cgaagaagct gtcaaggctt tggtggatac
     9361 caagattctc aaaccccaac gtggagagaa aaaacagctg gtcaatctag ccataaaaaa
     9421 tgcacgtgtt agtctggagc agaaatttaa cctgctagaa aaatcagtcg aaaagaccca
     9481 aggagctatt gaaaatctag gacgtttgct ccaaattccg actccagttc gcatcgagtc
     9541 cttcgataac tccaacatca tggggaccag tcctgtttca gccatggtgg tctttgttaa
     9601 cggcaggcca agtaaaaaag attaccgtaa gtataaaatc aagaccgtag tcgggccaga
     9661 cgactatgcc agtatgcgag aggtcattcg cagacgctat ggtcgagtac agcgtgaggc
     9721 tttgactcct ccagatttga ttgtgattga tggggggcaa ggtcaagtca atattgctaa
     9781 gcaagtcatc caagaagaac tgggcttgga tattccaatt gcaggtctgc aaaagaatga
     9841 taagcaccaa acccatgaat tgctctttgg agatccgctg gaagtggtgg acttgtctcg
     9901 caattctcag gaatttttcc tcctccaacg cattcaagat gaggtccacc gctttgccat
     9961 taccttccac cgccaactgc gctccaaaaa ttctttctca tctcaattgg atgggattga
    10021 tggtctggga cctaaacgca agcaaaatct catgaggcac ttcaagtctc tgaccaaaat
    10081 caaggaagcc agtgtggatg agattgtcga agttggagta ccaagagcag tagcagaggc
    10141 tgtgcaaacc aagttgaacc cgcaggaaac agaaatcttg cttcaagtgg cggaagaaag
    10201 agtagattac caaacggaag gaaaccacaa taaaccataa aatcgcaatt ttatcagatg
    10261 ttcatggcaa tgcgacggcg ctagaagcag tgattgcaga tgctaaaact caaggagcca
    10321 gtgaatattg gctcatggga gacattttcc tccctggtcc aggtgcaaat gacttggtcg
    10381 ctctgttaaa ggaacttcct atcacggcaa gtgttcgagg caattgggat gattgtgtcc
    10441 ttgaggcttt agatgggcaa tatggcttag aagacccaca ggaagttcag ctcttgcgta
    10501 tgacacagta tttgatggag cgaatggatc ctgcaacgat tgtctggcta cgaagcttgc
    10561 ctttgctgga aaagaaagaa attgacggat tgcgcttttc tatctctcat aatttacctg
    10621 acaaaaacta tggtggtgac ttgctagttg agaatgatac agagaaattt gaccaactgc
    10681 tagatgcgga aacggacgtg gcagtttatg gtcatgttca caagtagttg cttcgttatg
    10741 gtagtcaagg gcaacaaatc atcaatccag ggtcgattgg catgccctat tttaattggg
    10801 aggcgttaaa aaatcaccgt gcccagtatg ccgtgataga ggttgaagat ggggaattgg
    10861 taaatatcct atttcgtaaa gtcgcttatg attacgaggc tgagttagaa tttgccaaat
    10921 ccaagggttt tccctttatc gaaatgtatg aagaactacg tcgtgaagat aactatcaga
    10981 ggcacaatct ggaactatta gcaagcttaa tagaaaagca taggtatgta gaggatgtga
    11041 agaatttttt tgattttttg taagagtttc ctaaaataac caatgcaaac taaaaaagcg
    11101 attggctgat cccatcgctt ttaatatatc ttatactcaa tatgaaaatc aaagagcaaa
    11161 ctaggaagct agccgtaggt tgctcaaagc acagctttga ggttgcagat aaagctgacg
    11221 tggtttgaag agattttcga agagtgttat tgtaactgag attgatctgg gaggtaagaa
    11281 ccacctagat aggtattgct gagtttttca agggttccgt cttgatagag ttctttgagc
    11341 gctttatcaa attgctcttt aaactctttt tggtcgcttg agaaaatgat ataattgctg
    11401 gggctatctg cagaaggtaa atcaacgact gagaggtcta aaccacggtc cttgataatc
    11461 ttttgaacgg ataccttgtc aaaaactagg aaatcaaact ctccgttagc aaggtctagg
    11521 attcgtttac caatatcctc accagaaaaa ttaattgtag cgggattatc agtgtgtttc
    11581 tgattccagt tattgatgaa ttgagcgtta gaagttccgg tatcctcttg tgttgtttta
    11641 ccagcgatct ggtcaagaga agtcaaagga tttttcttgt tgctgacaag gacgagggga
    11701 ttgttggaaa tcggaagcga gtaaaggtat ttttcagcac gctcttttgt gtaactcaaa
    11761 ttattggccg cagcctgata gtgaccagaa tcaagtcctg ggaagatact ttcccaggcg
    11821 gttctttgaa attgaatctc gtagtcgctg agtttttcat ctactgcctt tagaacttcg
    11881 atatcgaagc ctgtcagatt gcctttatct tcgtagtcaa atggtggcac gtcgccagct
    11941 gtagcaagga cgattgtctt ttgagcgcta gtctctttgg gtgtagcttg attcccacag
    12001 gcaaccaaaa atggtaggat agctagtaat aggctaaatt ttttcatact gtctccattc
    12061 aaatgtaaag tacttgctag tttatcagaa actttcctga taaaccaata tatatctttt
    12121 atgtctgtga tagaaaaact taatcatgaa aaatccctgc aagctctttc aaattatggt
    12181 agaatggaag cagtagaatt agaaaaggaa atcgattatg aaatttcttg aattaaataa
    12241 aaaacgtcat gcgaccaagc atttcactga taagcctgtt gatccaaaag atgtgcgtac
    12301 ggctatcgaa attgcaacct tggcgccaag cgcccacaac agccagcctt ggaaatttgt
    12361 agttgttcgt gagaaaaatg ctgaactggc aaagttggct tatggttcaa actttgagca
    12421 ggtatcatca gcacctgtaa ccattgccct atttacagat acagaccttg ccaaacgcgc
    12481 tcgtaagatt gcccgagttg gtggtgctaa caatttctca gaagaacaac ttcaatactt
    12541 catgaaaaat ttgccagctg aatttgcacg ctacaatgaa caacaagtca gcgactacct
    12601 agctctcaat gcaggtttgg ttgccatgaa cttggttctt gcattgacag accaaggaat
    12661 tggttctaac attattcttg gttttgacaa atcaaaagtt aatgaagttt tggaaatcga
    12721 agaccgtttc cgcccagaac tcttgatcac agtgggttat acagacgaaa aattggaacc
    12781 aagctaccgc ttgccagtag atgaaatcat cgagaaaaga tagaaagaag aaaaaatgac
    12841 agcaattgat tttacagcag aagtagaaaa acgcaaagaa gacctcttgg ctgacttgtt
    12901 tagccttttg gaaatcaatt cagaacgtga tgacagcaag gctgatgccc agcatccatt
    12961 tgggcctggt ccagtaaaag ccttggagaa attccttgaa atcgcagacc gcgatggcta
    13021 cccaactaag aatgttgata actatgcagg acattttgag tttggtgatg gagaagaagt
    13081 tctcggaatc tttgcccata tggatgtggt gcctgctggt agcggttggg acacagaccc
    13141 ttacacacca actatcaaag atggtcgcct ttatgcgcgc ggggcttcgg acgataaggg
    13201 tcctacaaca gcttgttact atggtttgaa aatcatcaaa gaattgggtc ttccaacttc
    13261 taagaaagtt cgcttcatcg ttggaacaga cgaagaatca ggctgggcag acatggacta
    13321 ctactttgag cacgtaggac ttgccaaacc agatttcggt ttctcaccag atgctgaatt
    13381 tccaatcatc aatggtgaaa aaggaaatat cacggaatac ctccactttg caggagaaaa
    13441 tacaggtgtt gcccgtcttc acagctttac aggtggttta cgtgaaaata tggtaccaga
    13501 atcagcaaca gcagtcgttt caggtgactt ggctgacttg caagctaaac tagatgcctt
    13561 tgttgcagaa cacaaactta gaggagaact ccaagaagaa gctggcaaat acaaggtgac
    13621 gatcattggt aaatcagccc acggtgctat gcctgcttca ggtgtcaatg gcgcaactta
    13681 ccttgccctc ttcctcagtc agtttggctt tgctggtcca gccaaagact accttgacat
    13741 cgcaggtaaa attctcttga acgatcatga gggtgaaaat cttaagattg ctcatgtgga
    13801 tgaaaagatg ggtgctcttt ctatgaatgc cggcgtcttc cgcttcgatg aaacaagtgc
    13861 tgataatacc attgccctca acatccgcta tccaaaagga acaagtccag aacaaatcaa
    13921 gtcaatcctt gaaaacttgc cagttgtttc tgttagcctg tctgaacacg gtcacacgcc
    13981 tcactatgtg ccaatggaag atccacttgt gcaaaccttg ttgaatatct atgaaaaaca
    14041 aactggcttt aaaggtcatg aacaagtcat cggtggtgga acctttggtc gcttgttaga
    14101 gcgcggagtt gcctatggtg ctatgttccc agactcaatt gataccatgc accaagccaa
    14161 tgaatttatc gccttggacg atctcttccg agcagcagca atctatgccg aagctattta
    14221 cgaattgatc aaataaaacg atagaagtct gagatcttat gcttggactt ctttttggag
    14281 ggaaagtaga tgtctcaaat cgaaagaatc aaacaggcta tcatggcgga ttcacagaat
    14341 gccagctata cagagcgtgg cattgagcct ctctttgcag cgccaaaaac tgctcgcatc
    14401 aatatcatcg gtcaggctcc gggacttaaa actcaagaag caggccttta ctggaaagat
    14461 aaaagtggtg accgcttgcg ggactggcta ggtgtggatg aagatacctt ttacaattca
    14521 ggttattttg ctgttttgcc tatggatttc tactttccag gacatggcaa gtcgggtgat
    14581 cttccgcctc gtacaggttt tgcagaaaaa tggcatccgc agctcttaca ggaattgcct
    14641 gatattcagt taaccctctt gattgggcaa tatgcccaag cctactattt acaggagaaa
    14701 atcagtggga aggtaacgga gagggtgaaa cactataaag actatctgcc agcctatttt
    14761 ccgctagttc acccatcacc acgaaatcaa atctggatgg ccaaaaatcc ttggtttgag
    14821 gcagaagtag tgccagattt gaaaaaaaga attaaaacca ttttatagtc aatgaaaatc
    14881 aaagagcaaa ctaggaagct agtcgtaggc tgctcaaagt acagctttga ggttgcagat
    14941 aaaactgacg aagtcggtaa catacgcacg gtaaggcgac gctgacgtgg tttgaagaga
    15001 ttttcgaaga gtattagaag aaaaagaatg aaagaaatag cctttgacgc attttaccag
    15061 ctttaccaaa acgaccagct ttctctagtg gatgtgagag aagtggatga gtttgcagct
    15121 cttcatttag aatgtgccca caacctaccg cttagtcaat tggctgatag ttatgattaa
    15181 ttggacaaag atcgcttgca ttatattatt tgcaaatctg gaatgagatc ggcgcgtgct
    15241 tgccaattcc tgttagaaca aggttataat gttatcaatg tccagggtgg catgttagcc
    15301 tttgaagaac tttaaaattt tgcatttctc ctgcttggtg tggactgggt aggagagttt
    15361 tatttttaga taattcttat ttttaagaaa attgaaaaca tttaatattt gcctcgtgat
    15421 gcttttttca gactcctaat cgtggtatac taggtcagta ttttataaat atgaaggaga
    15481 tttttatggc taaaaaaggt accctaacag gtttgctcct gtttggaata ttttttggtg
    15541 cggggaactt gatttttccg ccttctctag gtgctctatc tggagaacat tttcttcctg
    15601 ccatcgcagg ttttgtcttt tcaggcgttg gtatcgccgt cttgaccctt attattggaa
    15661 cgctaaatcc taaaggatat atctacgaga tttcaacgaa gatagcgcct tggtttgcga
    15721 ctctttacct ctcagttctt tacttgtcaa tcggtccatt ctttgctacc ccacgtactg
    15781 ctacaacagc ttacgaagta gggattagcc cccttttgtc ggatgcaaat aaaggacttg
    15841 gcttgattgt atttacggtt ctgtattttg cggcagccta tttgatttcg cttaatccat
    15901 caaaaatctt agaccgcatt ggacgtgttt taacgccagt ctttgcaatt ttgattgtta
    15961 tcttggtcgt tctgggagct atcaaatatg gtggaacaag tcctcaagct gcttcagctg
    16021 cttatcaagc ttctgccttt ggtacaggtt tcctagaagg ttacaatacc ttggacgccc
    16081 ttgcttcagt ggcctttagc gtaatcgcag ttcaaacctt gaaacaactt ggattttcaa
    16141 gtaagaaaga atacatttca actatttggg ttgttggtat cgttgttgcc cttgccttca
    16201 gcgctcttta catcggttta ggttttcttg gaaatcattt cccagtacca gctgaagcga
    16261 tgaagggtgg aacaccaggt gtttacatct tgtcacaagc cactcaagaa atctttggct
    16321 caacagctca actcttcctt gcagctatgg ttaccgtaac ctgcttcaca acgactgttg
    16381 gtttgattgt gtcaacagct gagttcttta atgagcgctt cccacaaatc agctacaagg
    16441 tttatgcgac agcctttacc ttgattggat ttgctattgc caatttgggt cttgatgcga
    16501 ttatcaagta ctcaattcca gtactggtta tcttgtaccc aatcacgatt gctatcgtta
    16561 tgattgtcat tgtcaacaaa tttgttgccc tttcaaaacc aggtatgcag ttgacaattg
    16621 ctgtggttac agttattgcc attgcaagcg tactaggaag ctcgtttaag gttgagtttc
    16681 ttgcaaatct tgttagcgtt cttccttttg ccaaggcatc tctcccatgg ttggtgccag
    16741 ccattgttgg aatcttgctc tcattggttc taccaaacaa gcaagaaagc gatgtttttg
    16801 aaatggaata atcacttaaa tcacttttgt agccaagtct acaggagtga ttttcttttt
    16861 ttatccgatg ataaatgtgt tataataggt agcgaaagag gtgaagaaat gaatcaaaca
    16921 gtagaatata tcaaagaact gacagccatc gcatcgccaa cgggcttcac tcgtgaaatt
    16981 tcgaattatt tagtcaagac tctagaaggt tttggttacc agccagttcg cacagctaag
    17041 ggtggtgtca atgtgaccat caaaggtcaa aatgatgagc aacatcgcta tgtgactgcc
    17101 catgtggata cgctgggtgc tattgttcgt gctgtcaaac cagatggtcg tcttaaattg
    17161 gaccgtatcg gtggttttcc ttggaacatg attgaaggtg aaaactgtac cgttcatgtg
    17221 gctagcacag gtcaaaaggt atcaggaacc atcctcatcc accaaacttc ttgccatgtc
    17281 tacaaggatg caggaactgc agaacgcacg caggacaata tggaagtgcg tttggatgta
    17341 aaagtaagca atgaaaaaga aactcgtgct cttgggattg aggtcggtga ttttatcagc
    17401 ttcgatacac gaactgttgt gactgataca ggttttatca agtctcgtca tttggatgat
    17461 aaggtcagcg cagcgatttt gctcaacctg cttcgtattt ataagaaaga gaagattgaa
    17521 ttgccagtaa caactcattt tgctttttca gtttttgaag aagtaggaca tggtgctaat
    17581 tctaacattc ctgctcaggt agtagaatat ctggctgtgg atatgggagc tatgggagat
    17641 gaccagcaga cagatgagta cacagtatct atctgtgtca aggatgcttc aggtccttat
    17701 cactatgact tccgtcaaca cttggttgcc ttggctaaag agcaagatat tccatttaag
    17761 ctcgacatct atccatttta cggatcggac gcttcagcag cgatgtcagc aggtgcagag
    17821 gtcaagcatg cacttcttgg agctggtatt gagtccagtc actcttatga gcgcacccac
    17881 atcgattcag taatagcgac tgagcgtatg gttgatgctt atcttaagag cgaggcggta
    17941 tcatagaatg tgcttaatct gtcagagaat cgaactcatt aaagctgggc aaaatcccta
    18001 ctttgtaaaa gaactagaaa caggctatgt tgttattggt gactaccaat actttaaggg
    18061 ctatacctta tttttagcaa aagatcatgt cacagaactc catcatatgg aaacttctgt
    18121 aaaacttcgc tttttagagg aaatgagttt agtccaagaa gcagtcgcca aagcatttga
    18181 ggctgaaaag atgaatattg aactcctagg aaatggcgat gcccacgctc attggcatct
    18241 ttttccccga agagcaggtg atatgaagag tcacggtttg aatggccgtg gcccagtctg
    18301 gtgggtgccc tgggaagaaa tggcggcaga agattgccaa gtacaatcgc cagagctgga
    18361 agagatgatt aaaatcttat ctcatgaatt ggagaagtat ttggcctaag agagaggaag
    18421 aaagaaatga aaaaaagata tgtcatttta tctggtttac tagcattgac ccttgcagct
    18481 tgttcgcaag aaaaaaccaa agttgaagaa aacactcaaa aaacggagca aagcagtcaa
    18541 ccggaaggaa ctgtaggtag caaatctcaa gcttctagtc agaaaaaggc agaagttagc
    18601 aataaaggga gctattatag tatccaaggg aaatacgatg agattatcct agctaataaa
    18661 cgttatcctt tgtcaaaaga ttacaatcca ggagaaaatc caacagccaa ggcagagttg
    18721 ctcaaactaa tcgcagctat gcaggctgag ggctatccaa tcagtgacca atacagtggg
    18781 tttaggagtt atgaaactca ggctaagctc taccaagact atgtcaatca agatggtaag
    18841 gaagcagcag atcgctactc agcacgccca ggttacagtg aacaccagac gggtcttgct
    18901 tttgatttga ttgggacaga tggggattta gttactgaag aaaaggcagc ccagtggctc
    18961 ttggaccatg cggctgacta tggctttgtt gttcgctatc ttaaaggcaa ggaaaaggag
    19021 actggctaca tggcagaaga atggcatctc cgctacgttg gaaaagaagc caaagaaatt
    19081 gctgcaagtg gtctcagttt ggaagagtac tatggctttg aaggcggcga ttatgtcgat
    19141 taaaaatgaa agttttctct tgcattttta gataaatagt gtataatgga aaggtatgtg
    19201 taaagcatac ttgtgggagg taaaaatctc taattaccgc caaaaccaca aaggaggatt
    19261 taaaaatggc taaaaaagtc gaaaaacttg taaaattgca aatccctgct ggtaaagcta
    19321 caccagctcc accggttgga cctgctcttg gtcaagctgg tatcaacatc atgggattca
    19381 caaaagagtt caacgctcgt acagctgacc aagctggtat gatcattcca gttgttatct
    19441 cagtttacga agataaatca tttactttcg ttacgaaaac accaccagct gctgttcttt
    19501 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taataaaact aaagttgcta
    19561 cagttactcg tgcacaagta caagaaattg cagaaactaa gatgccagat ttgaacgcag
    19621 caaacgtaga gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
    19681 ttgttgacta atcaataaca cagtagaaaa tctcacagca gtctattagt tgcttttcat
    19741 actaggcaag tgactgaggc ttgtacttgg atacagcaag ggagcttaag gccgtaggaa
    19801 gagaaaaata gtagactgaa aacccgcaag acttcatcat ttcgagaagt gacgtgggag
    19861 atgaaaatcg attgaaccac ttacaaggag aatagaaaat ggctaaaaaa agcaaacaac
    19921 ttcgtgctgc tcttgagaaa atcgacagca caaaagcata cagtgtagaa gaagctgtag
    19981 cacttgcaaa agaaactaac tttgcaaaat ttgatgcaac tgtagaagtt gcttacaact
    20041 tgaacatcga cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta ttgccaaacg
    20101 gtactggtaa aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct gaagaagcaa
    20161 aagctgctgg tgcagacttt gttggtgaag atgaccttgt tgctaaaatc aacgacggtt
    20221 ggttggactt cgacgtagtt atcgctacac ctgatatgat ggctcttgtt ggacgtcttg
    20281 gacgtgtcct tggaccacgt aacttgatgc caaaccctaa aactggtact gtaacaatgg
    20341 atgttgctaa agcagttgaa gagtctaaag gtggtaaaat cacttaccgt gctgaccgtg
    20401 caggtaacgt tcaagcaatc atcggtaaag tatcatttga agctgaaaaa ttggttgaaa
    20461 actttaaagc tttcaacgaa acaatccaaa aagtaaaacc agctacagct aaaggaactt
    20521 acgtaacaaa cttgactatc acaactactc aaggtgttgg tatcaaagtt gacgtaaact
    20581 cactttaatc agtagttaaa gtaatgtaaa aaagttgaag acactatgtc tcaacttttt
    20641 ttgatgtacg acgggcatgt cgtatatctg aggtgtaagt cctcggtggg cacctactac
    20701 cggtgaaccc aatagcgatt cccaagtctg acgctggaaa taagaatcgt cagaggaagg
    20761 gatagcgaaa tcgtggctct acgaacagga acgtgataat aaggcctata tagcggataa
    20821 ggtatctggc tggggcgacc actatcaatt ataataaaat gaaataagaa taggacgaat
    20881 cgttcaggac agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta
    20941 gtttcaatct actatagtag ctcagaagtc ggtacttaaa cgtgctatat caaaaccagt
    21001 ccttgaaaaa cgtggactgg tttcgtgttt ggattattac cttgaacgac atgcgttaaa
    21061 agttagttga
//
LOCUS       DBERYS010000035        20752 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.035, whole genome shotgun sequence.
ACCESSION   DBERYS010000035 DBERYS010000000
VERSION     DBERYS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.035"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(117..1037)
                     /gene="cysK"
                     /locus_tag="ACL8LA_001461"
     CDS             complement(117..1037)
                     /gene="cysK"
                     /locus_tag="ACL8LA_001461"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGS1813206.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYNGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1322..1957)
                     /locus_tag="ACL8LA_001462"
     CDS             complement(1322..1957)
                     /locus_tag="ACL8LA_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGS1813207.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDNGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2013..3311
                     /locus_tag="ACL8LA_001463"
     CDS             2013..3311
                     /locus_tag="ACL8LA_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1813208.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3308..3970
                     /locus_tag="ACL8LA_001464"
     CDS             3308..3970
                     /locus_tag="ACL8LA_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGS1813209.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREEGASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4050..4598
                     /gene="raiA"
                     /locus_tag="ACL8LA_001465"
     CDS             4050..4598
                     /gene="raiA"
                     /locus_tag="ACL8LA_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGS1813210.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4734..6707
                     /locus_tag="ACL8LA_001466"
     CDS             4734..6707
                     /locus_tag="ACL8LA_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGS1813211.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6704..7156
                     /gene="rplI"
                     /locus_tag="ACL8LA_001467"
     CDS             6704..7156
                     /gene="rplI"
                     /locus_tag="ACL8LA_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGS1813212.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7200..8552
                     /gene="dnaB"
                     /locus_tag="ACL8LA_001468"
     CDS             7200..8552
                     /gene="dnaB"
                     /locus_tag="ACL8LA_001468"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGS1813213.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEICSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8554..8820
                     /locus_tag="ACL8LA_001469"
     CDS             8554..8820
                     /locus_tag="ACL8LA_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGS1813214.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            8909..10027
                     /gene="cbpD"
                     /locus_tag="ACL8LA_001470"
     CDS             8909..10027
                     /gene="cbpD"
                     /locus_tag="ACL8LA_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGS1813215.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGLWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10110..10212
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10283..10573
                     /locus_tag="ACL8LA_001471"
     CDS             10283..10573
                     /locus_tag="ACL8LA_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGS1813216.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10536..11294
                     /locus_tag="ACL8LA_001472"
     CDS             10536..11294
                     /locus_tag="ACL8LA_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813217.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11332..12339
                     /locus_tag="ACL8LA_001473"
     CDS             11332..12339
                     /locus_tag="ACL8LA_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1813218.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12339..13067
                     /locus_tag="ACL8LA_001474"
     CDS             12339..13067
                     /locus_tag="ACL8LA_001474"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813219.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13186..13644
                     /locus_tag="ACL8LA_001475"
     CDS             13186..13644
                     /locus_tag="ACL8LA_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGS1813220.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            13646..16078
                     /locus_tag="ACL8LA_001476"
     CDS             13646..16078
                     /locus_tag="ACL8LA_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGS1813221.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16399..17052
                     /gene="rr06"
                     /locus_tag="ACL8LA_001477"
     CDS             16399..17052
                     /gene="rr06"
                     /locus_tag="ACL8LA_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGS1813222.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17049..18380
                     /gene="hk06"
                     /locus_tag="ACL8LA_001478"
     CDS             17049..18380
                     /gene="hk06"
                     /locus_tag="ACL8LA_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGS1813223.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSRLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            18463..18990
                     /locus_tag="ACL8LA_001479"
     CDS             18463..18990
                     /locus_tag="ACL8LA_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGS1813224.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19069..>20752
                     /gene="pspC"
                     /locus_tag="ACL8LA_001480"
     CDS             19069..>20752
                     /gene="pspC"
                     /locus_tag="ACL8LA_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HGS1813225.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSEN
                     NPTVTSSGQDISKKYADEVKSHLEKILSEIQTNLDRSKHIKTVNLINKLQDIKRTYLY
                     ELNVLEDKSKAELPSKIKAELDAAFEQFKKDTLPTEPGKKVAEAKKKVEEAEKKAKAQ
                     KEEDYRNYPTITYKTLELEIAESDVKVKEAELELVKKEADESRNEGTINQAKAKVESE
                     QAEATRLKKIKTDREKAEEEAKRRADAKEQDESKRRKSRVKRGDLGEPATPDKKENDA
                     KSSDSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKDQKEEDHRNYPTITYKTLEL
                     EIAESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAE
                     EEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEED
                     YARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQYNG
                     SWYYLNANGDMATGWFQYNGSWYYLNANGSMATGWLQYNGSWYYLNANGSMAT"
BASE COUNT         6554 a         3426 c         4738 g         6034 t
ORIGIN      
        1 tatttaccat ggactaaaat tgtacaagaa aagtgcaaat aagaaatctc cagattagga
       61 actatccgtg agttctctag tctggagatt tttcaatata cttcgttatt ggacggttac
      121 aattcataaa gtgctgtaga gagataacgt tcaccgttat ctggtgctag ggcaaggact
      181 tttttacctg tacctaattt tttggcaacc tcgatggctc cgtagatagc tgcagctgag
      241 gaaatcccta caaggaagcc ttcttttcca ccaatttcac gtccgagtgc aagagcgtca
      301 tctgatgtta cacgaacgat accattatag gctttagtat caagtgtatc aggaataaat
      361 ccagctgaga taccttgaat tttgtgagga ccaggttttt caccagatag aatagcagat
      421 tcatctgctt ctactgcaaa aacttgaatg ttagaatttt ctgatttgag tgcatgagaa
      481 acaccagaaa tcgttccacc ggtacctact ccagcaacaa aggcatctaa tccatcttta
      541 ccgaaagcag ctagtatctc agctcctgtt gttctttcgt gtacttctgg attagctgga
      601 ttgtcaaatt gaagaggaag gaaaccatca cgttcagcag cgatttcttg agccttagca
      661 atagcacctt tcattccctc gctaccagga gttaggacga gttcagcacc ataagcttgg
      721 ataattttac gtcgttctac actcatagtt tcaggcataa cgatgacgac tttataccct
      781 ttagcagcac ctacccatga aagtccaata ccagtgtttc cacttgttgc ttcaacaata
      841 gtagaaccag gtttcagaat accatcttgt tcagcttttt caatcatgct aagggcaata
      901 cggtctttta cagatgaacc aggattaaat gcttcaagct ttatatagac gtctgcagca
      961 ccttctggca cgatgttgtt aagtttaaca atcggtgttt gaccgattaa ttcagtaatg
     1021 ttgttataaa tagacatatg aatacctctt tgtataagat atctttagta taacgctatc
     1081 cataccattt gtaaaatata gaaatcctat cgaagcgata ggatttctat atatcataga
     1141 tcaaagtcat taattttcaa gtgattgcgt tcagctaatt ctagtaaata ggtgtgaagg
     1201 aaaacgctat caggtttcgt ttgaattgct tcattttcta gtatattttg gtacgcatct
     1261 ttttattttg ctcgctaaag tagttgtgag aatgtcaacg aaacgctgta ttattcattg
     1321 tttacactaa gtttacagga acttcaacct ctcgtaaacc ttggtcagtt aaagtgactt
     1381 ttccattaaa aaactccaca agtgcagctt taatagtttc tttttcttct ttatcaacat
     1441 aaatcatcgt atcgacttga tctgtaaagt ttgtatccag ctccatgaga tcatgttctt
     1501 taaggaagtt actgtactct tggtactgag cataagacat ttgaatagca atgccagcct
     1561 gttcttttat ttcaataata ccaatttctt tgacagctaa ggcgacactg ccggcgtaag
     1621 cacgaattag tcctccagcg cctagtttaa taccaccaaa gtagcgtgtc acgaccacac
     1681 agacattggt gagattgtga ttttctagta ccccaagcat gggaacacca gcagtaccac
     1741 taggctcacc attatcactt gtacgtttaa tttcactacg ttctccaata ataaaggcag
     1801 agcagttatg tgtcgctttg tagtgttctt ttttgatggc agtaatgaag tcacgagcct
     1861 cttcttcgct ataaacacgc ttggcatggc agataaagcg agattttttt atttcttctt
     1921 ggacttggcc gtcctcttta attgtcctaa attccatgat ttaattgtac taaaaaataa
     1981 tctaaagtgc tagtttttac gaatagagaa gtatgaaagt aaatttagat tatctcggtc
     2041 gtttatttac tgagaatgaa ttaacagaag aagaacgtca gttggcggag aaacttccag
     2101 caatgagaaa ggagaagggg aaacttttct gtcaacgttg taatagtact attctagaag
     2161 aatggtattt gcccatcggt gcttactatt gtcgagagtg cttgctgatg aagcgagtca
     2221 gaagtgatca aactttatac tattttccgc aggaggattt tccgaagcaa gatgttctca
     2281 aatggcgcgg ccaattaact ccttttcaag agaaggtgtc agagggactg cttcaagcag
     2341 tagacaagca aaagccaacc ttagttcatg cggtaacagg agctggaaag acagaaatga
     2401 tttatcaagt agtggctaaa gtgatcaatg cgggtggtgc agtgtgtttg gctagtcctc
     2461 gcatagatgt ttgtttggag ctgtacaagc gcctgcaaca ggatttttct tgcgggatag
     2521 ctttgctaca tggagaatcg gaaccttatt ttcgaacacc actagttgtt gcaacaaccc
     2581 atcagttatt gaagttttat caagcttttg atttgctgat agtggatgaa gtagatgctt
     2641 ttccttatgt tgataatccc acgctttacc acgctgtcaa gaatagtgta aaggagaatg
     2701 gattgagaat ctttttaaca gcgacttcga ccaatgagtt agataaaaag gtccgtttag
     2761 gagaactaaa aagactgagt ttaccgagac ggtttcatgg aaatccgttg attattccaa
     2821 aaccaatttg gttatcggat tttaatcgct acttagacaa gaatcgtttg tcaccaaagt
     2881 taaagtccta tattgagaag cagagaaaga cagcttatcc gttactcatt tttgcttcag
     2941 aaattaagaa aggggagcag ttagaagaaa tcttacagga gcaatttcca aatgagaaaa
     3001 ttggctttgt atcttctgta acagaggatc gattagagca agtacaagct tttcgagatg
     3061 gagaactgac aatacttatc agtacgacaa tcttggagcg tggagttacc ttcccttgtg
     3121 tggatgtttt cgtagtagag gccaatcatc gtttgtttac caagtctaat ttgattcaga
     3181 ttggtggacg agttggacga agcatggata gaccgacagg agatttgctt ttcttccatg
     3241 atgggttaaa tgcttcaatc aagaaggcga ttaaggaaat tcagatgatg aataaggagg
     3301 ctggtctatg aagtgcttgt tatgtgggca gactatgaag actgttttaa cttttagtag
     3361 tctcttactt ctgaggaatg atgactcttg tctttgttca gactgtgatt ctacttttga
     3421 aagaattggg gaagagaact gtccaaattg tatgaaaaca gagttgtcaa caaagtgtca
     3481 agattgtcaa ctttggtgta aagaaggagt tgaagtcagt catagagcga tttttactta
     3541 caatcaagct atgaaggatt ttttcagtcg gtataagttt gatggagact tcctgttaag
     3601 aaaagttttc gcttcatttt taagtgagga gttgaaaaag tacaaagagt atcaatttgt
     3661 tgtaattccc ctaagtcctg atagatatgc taatagagga tttaatcagg ttgagggctt
     3721 ggtagaggca gcaggctttg agtatctgga tttattagag aaaagagaag agggagccag
     3781 ttcttctaaa aatcgttcag agcgcttggg gacagaactt cctttcttta ttaaaagtgg
     3841 agtcactatt cctaaaaaaa tcctacttat agatgatatc tatactacag gagcaactat
     3901 aaatcgtgtt aagaaactgt tggaagaagc tggtgctaag gatgtaaaaa cattttccct
     3961 tgtaagatga ggaaaaaatt tgcaaaaata gaatgaaagc gttataataa aatcataaaa
     4021 acaaaaatgt ttagaaagaa ggtactcata tgattaaata tagtatccgt ggtgaaaacc
     4081 tagaagtaac agaagcaatt cgtgattatg tagtttctaa actcgaaaag atcgaaaagt
     4141 acttccaacc agaacaagag ttggatgccc gaattaactt aaaagtttat cgtgaaaaaa
     4201 cggctaaagt ggaagtaacg attccgcttg gatctattac tctccgcgca gaagatgtat
     4261 ctcaagatat gtatggttca attgaccttg taactgataa aattgaacgt cagattcgta
     4321 aaaataaaac aaaaatcgag cgtaaaaata aaaataaggt agcaactggt caattattta
     4381 cagatgcttt ggtggaagat tcaaatgttg tccagtctaa agttgttcgt tcaaaacaaa
     4441 ttgatttaaa accaatggat ttggaagaag caattctaca aatggattta ttggggcatg
     4501 atttctttat ctatgtggat gttgaagatc aaacaaccaa tgtgatttat cgtcgtgagg
     4561 atggcgaaat tggtttgtta gaggttaaag aatcttaatt tcaatatgtg taaaggtagg
     4621 tttactgaat tgtaaactac ctttttctta taattgatag aattactgat tacactttta
     4681 aaaagtcgct ttttgagggg tattatggta taatgggtgc caagaggttt tgaatgaaaa
     4741 aattttatgt aagtccaatt tttcctattc tagtaggatt gattgcgttt ggagtcttat
     4801 ccactttcat tatttttgtt aataataatc tgttgacggt tttaattttg tttctttttg
     4861 taggaggcta tgttttttta tttaagaaac tgagagtgca ttatacaagg agtgatgtag
     4921 aacagataca gtatgtaaac caccaagcgg aagaaagttt gacagctcta ttggaacaga
     4981 tgcctgtagg tgttatgaaa ttgaatttat cttctggaga ggttgagtgg tttaatccct
     5041 atgctgaatt gattttgacc aaggaagatg gtgattttga tttagaagct gttcaaacga
     5101 ttatcaaggc ttcagtagga aatccgtcta cttatgccaa gcttggtgag aagcgttatg
     5161 ctgttcatat ggatgcttct tccggtgttt tgtattttgt agatgtatcc agggaacaag
     5221 ccataacaga tgaattggta acaagtcgac cagtgattgg gattgtctct gtggataatt
     5281 atgatgattt ggaggatgca acttctgagt cagatattag tcaaatcaat agttttgtag
     5341 ctaattttat atcagagttt tcagaaaaat acatgatgtt ttctcgtcgg gtaagtatgg
     5401 atcgatttta tctatttact gactacacgg tgcttgaggg cttgatgaat gataaatttt
     5461 ctgttattga tgctttcaga gaagagtcga aacagagaca gttgcccttg accttaagta
     5521 tgggattttc ttatggcgat ggaaatcatg atgagatagg gaaagttgct ttgctcaatt
     5581 tgaacttggc tgaagtacgt ggtggcgacc aggtggttgt taaggaaaac gacgaaacga
     5641 aaaatccagt ttattttggt ggtgggtctg ctgcttcaat caagcgtaca cggactcgta
     5701 cgcgcgctat gatgacagct atttcagata agattcggag tgtagatcag gtttttgtag
     5761 tcggtcacaa aaatttagac atggatgctt tgggctctgc tgtaggtatg cagttgttcg
     5821 ccagcaatgt gattgaaaat agctatgctc tttatgatga agaacaaatg tctccagata
     5881 ttgaacgagc tgtttcattc atagaaaaag aaggagttac gaagttgttg tctgttaagg
     5941 atgcaatggg gatggtgacc aatcgttctt tgttgattct tgtagaccat tcaaagacag
     6001 ccttaacatt atcaaaagaa ttttatgatt tatttaccca aaccattgtt attgaccacc
     6061 atagaaggga tcaggatttt ccagataatg cggtcattac ttatatcgaa agtggtgcaa
     6121 gtagtgccag tgagttggta acggaattga ttcagttcca gaattctaag aaaaatcgtt
     6181 tgagtcgtat gcaagcaagt gttttgatgg ctggtatgat gttggatact aaaaatttca
     6241 cctcgcgagt aactagtcgg acatttgatg ttgctagcta tctcagaacg cgcggaagtg
     6301 atagtattgc tatccaggaa atcgctgcga cagattttga agaatatcgt gaggtcaatg
     6361 aacttatttt acaggggcgt aaattaggtt cagatgtact aatagcagag gctaaggaca
     6421 cgaaatgcta tgatacagtt gttattagta aggcagcaga tgccatgtta gccatgtcag
     6481 gtattgaagc gagttttgtt cttgcgaaga atacacaagg atttatctct atctcagctc
     6541 gaagtcgtag taaactgaat gtacaacgga ttatggaaga gttaggcggt ggaggccact
     6601 ttaatttggc agcagctcaa attaaagatg taaccttgtc agaagcaggt gaaaaactga
     6661 cagaaattgt attaaatgaa ataaaggaaa aggagaaaga agaatgaaag taatcttttt
     6721 agcagatgtt aaaggaaaag gtaaaaaagg cgaaattaag gaagtaccaa cagggtatgc
     6781 gcaaaacttt cttatcaaaa agaatctagc caaagaagcg actgctcaag ctgtaggtga
     6841 acttcgtggt aaacaaaaat cggaagaaaa agctcacgct gagatgattg cagaaggaaa
     6901 agcaattaaa gcacaacttg aagcagaaga aactgttgta gaatttgttg aaaaagttgg
     6961 tccagatggt cgtacctttg gttctattac caataagaag attgcagaag aattgcaaaa
     7021 gcaatttgga attaagattg ataaacgtca tattcaagta caagctccga ttcgagcggt
     7081 tggtttgatt gatgtgccag tgaaaatcta tcaagatatc acaagtgtaa tcaatcttcg
     7141 tgtgaaagaa ggataagttt acaccttctt gacaagattg taaaaggaag ggaagtctga
     7201 tggcagaagt agaagagtta cgagtacaac ctcaagatat cttagctgag caatccgttt
     7261 taggggctat ctttattgat gagagtaaac ttgtttttgt gcgagaatac attgagtctc
     7321 gggacttttt taagtatgcc catcgtttga ttttccaagc catggtcgat ttatccgatc
     7381 gtggtgatgc catagatgca acaacggttc gtactatcct tgataatcaa ggtgatttac
     7441 agaatattgg tggcttgtct tacttggttg agattgttaa ttctgtgcca acttctgcta
     7501 atgcggagta ttatgctaag attgttgcag aaaaagcaat gctacgtcgt ttaattgcca
     7561 agttgacaga gtctgtcaac caagcttacg aagcgtcaca accagctgat gaaattattg
     7621 ctcaggcaga aaaagggctg attgatgtca gtgaaaatgc aaatcgaagc gggtttaaga
     7681 acattcgaga tgtgttgaat ctcaactttg gaaatctgga agctcgctcg caacaaacga
     7741 ccgatattac aggtattgcg acaggttatc gtgatttgga tcatatgaca acaggacttc
     7801 atgaggagga gttgattatc ttagcagctc gtccagcagt tggtaagaca gcatttgcct
     7861 tgaatatcgc tcagaacatt gggactaagt tggacaaaac ggttgctatt ttttcactcg
     7921 aaatgggtgc ggaaagcttg gtagatcgta tgttagctgc agaaggcttg gtggagtcac
     7981 attctatccg tacagggcaa ttgacagatg aggagtggca aaaatatact attgctcagg
     8041 gtaatctagc taacgccagt atctatatcg atgatacgcc aggtattcgg attacagaga
     8101 tttgttctcg ttctcgtaaa ttggctcaag aaactggaaa tcttggtttg attttgatag
     8161 actatttaca acttatcacg ggaactggtc gagaaaatcg tcaacaagaa gtttctgaaa
     8221 tttctcgtca gttgaaaata ctagccaagg aattgaaggt tccagtaatc gctctgagtc
     8281 agctttctcg tggtgtagaa caacgtcagg acaagagacc ggtcttgtct gatattcgtg
     8341 aatctgggtc tattgagcag gacgctgata tcgtagcttt tctctatcgc gatgactact
     8401 atgaacgtgg tggtgaagaa gaggagggta tcccaaataa taaggtggaa gttattatcg
     8461 agaaaaaccg tagtggagct cgtggaacag tggaattgat tttccaaaaa gaatacaata
     8521 aattttcaag tatctcaaag agggaggcat aagatgtcag atgcatttac agatgtagcc
     8581 aagatgaaaa aaatcaaaga agaaatcaag gcacatgagg gacaagtcgt agaaatgact
     8641 ttggagaatg gtcgtaagcg ccaaaaaaat agattgggta agctaattga agtttatcca
     8701 tccctattta ttgtggagtt tggggatgtg gaaggagata aacaagttaa tgtttacgtt
     8761 gaatccttta cttactcaga tattcttaca gaaaagaatt tgattcatta tcttgactaa
     8821 agtgagaaat tttctcactt tttctttttt ctccgaataa tttaggtgaa ggcaatcatc
     8881 gctttatatt atttttcaag gaggaagaat gaaaatttta ccgtttatag caagaggaac
     8941 aagttattac ttgaagatgt cagttaaaaa gcttgttcct tttttagtag taggattgat
     9001 gctagcagct ggtgatagtg tctatgccta ttccggagga aatggatcga ttgcgcgtgg
     9061 ggatgattat cctgcttatt ataaaaatgg gagccaggag attgatcagt ggcgcatgta
     9121 ttctcgtcag tgtacttctt ttgtagcctt tcgtttgagt aatgtcaatg gttttgaaat
     9181 tccggcagct tatggaaatg cgaatgaatg gggacatcgt gctcgtcggg aaggttatcg
     9241 tgtagataat acaccgacga ttggttccat tacttggtct actgcaggaa cttatggtca
     9301 tgttgcctgg gtgtcaaatg taatgggaga tcagattgag attgaggaat ataactatgg
     9361 ttatacagaa tcctataata aacgaattat aaaagcaaac acgatgacag gatttattca
     9421 ttttaaagat ttggatggtg gcagtgttgg gaatagtcaa tcctcaactt caacaggcgg
     9481 aactcattat tttaagacca agtctgctat taaaactgaa cccctagtta gtgcaactgt
     9541 gattgattac tattatcctg gagagaaggt tcattatgat caaattctcg aaaaagacgg
     9601 ctacaagtgg ttgagttata cggcttataa cggaagtcgt cgctatatac agctagaggg
     9661 agtgacttct tcacaaaatt atcagaatca atcaggaaat atctctagct atggatccaa
     9721 taatagttca actgtcggtt ggaagaaaat aaatggtagt tggtatcatt tcaaatcaaa
     9781 tggttctaaa tcaacaggat ggctgaaaga cggttctagc tggtattatt tgaaattatc
     9841 tggtgaaatg cagacaggat ggttaaagga aaatggtttg tggtattatc tgggtagttc
     9901 aggggcaatg aaaacaggct ggtaccaggt ctctggtaag tggtattatt cttactcttc
     9961 aggcgcctta gctgttaata cgacggtgga tggctacaga gtaaacagtg atggagaacg
    10021 agtatagaaa attggagtag gagaaatttc ctgctctttt ctgtaagcag tttccttgac
    10081 atttttctta ttttgcgcta tcatatatac atataatata tgggagtctg tgtacagtct
    10141 gagaggaagt gttaaacttc gaccgcacct gatctgggta atgccagcgg agggaacgat
    10201 acttagtcta attttgcacc ttttccatgt atggtaaagg tttttctttt ttttaaaagg
    10261 aaaacgagaa gaggaggttc ttatgaaagc aagcattgcc ttgcaagttt tacccctagc
    10321 acaggggatt gatcggatag ctgttattga tcaggtcatt gcttatctgc aaactcaaga
    10381 agtgacgatg gtagtgacac catttgaaac ggtcttggaa ggggagtttg atgagcttat
    10441 gcgcattcta aaagaagcgc tggaagtggc agggcaggag gcagacaatg tctttgccaa
    10501 tgtcaaaata aatgtaggag agattttaag tattgatgag aaacttgaaa agtatactga
    10561 gacgacacat tagtctattg ggctttctcg gagtattgtc aatctggcag ttagcaggtt
    10621 ttcttaaact tctccccaag tttatcctgc cgacacctct tgaaattctc cagccctttg
    10681 ttcgtgacag agaatttctc tggcaccata gctgggcgac cttgagagtg gctttactgg
    10741 ggctgatttt gggagttttg attgcctgtc ttatggctgt gctcatggat agtttgactt
    10801 ggctcaatga cctgatttac cctatgatgg tggtcattca gaccattccg accattgcca
    10861 tagctcctat cctggttttg tggctgggtt atgggatttt gcccaagatt gtcttgatta
    10921 tcttaacaac aacctttccc atcatcgtta gtattttgga cggttttagg cattgcgaca
    10981 aggatatgct gactttgttt agtctgatgc gagcaaaccc ttggcaaatc ctgtggcatt
    11041 ttaaaatccc ggttagcctg ccttactttt atgcaggtct gagggtcagt gtctcctacg
    11101 cctttatcac aactgtggta tctgagtggt tgggaggttt tgaaggtctt ggtgtttata
    11161 tgattcagtc taaaaaactg tttcagtatg ataccatgtt tgccattatt attctggtgt
    11221 cgattatcag tcttttgggt atgaagctgg tcgatatcag tgaaaaatat gtgattaaat
    11281 ggaaacgtac ttaaaataga gcgtttttga aaaagaaaag aggaaatcaa aatgaagaaa
    11341 acatggaaag tgtttttaac gcttgtaaca gctcttgtag ctgttgtgct tgtggcctgt
    11401 ggtcaaggaa ctgcttctaa agacaacaaa gaggcagaac ttaagaaggt tgactttatc
    11461 ctagactgga caccaaatac caaccacaca gggctttatg ttgccaagga aaaaggttat
    11521 ttcaaagaag ctggagtgga tgttgatttg aaattgccac cagaagaaag ttcttctgac
    11581 ttggttatca acggaaaggc accatttgca gtgtatttcc aagactacat ggctaagaaa
    11641 ttggaaaaag gagcaggaat cactgccgtt gcagctattg ttgaacacaa tacatcagga
    11701 atcatctctc gtaaatctga taatgtaagc agtccaaaag acttggttgg taagaaatat
    11761 gggacatgga atgacccaac tgaacttgct atgttgaaaa ccttggtaga atctcaaggt
    11821 ggagactttg agaaggttga aaaagtacca aataacgact caaactcaat cacaccgatt
    11881 gccaatggcg tctttgatac tgcttggatt tactacggtt gggatggtat ccttgctaaa
    11941 tctcaaggtg tagatgctaa cttcatgtac ttgaaagact atgtcaagga gtttgactac
    12001 tattcaccag ttatcatcgc aaacaacgac tatctgaaag ataacaaaga agaagctcgc
    12061 aaagtcatcc aagccatcaa aaaaggctac caatatgcca tggaacatcc agaagaagct
    12121 gcagatattc tcatcaagaa tgcacctgaa ctcaaggaaa aacgtgactt tgtcatcgaa
    12181 tctcaaaaat acttgtcaaa agaatacgca agcgacaagg aaaaatgggg tcaatttgac
    12241 gcagctcgct ggaatgcttt ctacaaatgg gataaagaaa atggtatcct taaagaagac
    12301 ttgacagaca aaggcttcac caacgaattt gtgaaataat gacagaaatt agactagagc
    12361 acgtcagtta tgcctatggt caggagagga ttttagagga tatcaaccta caggtgactt
    12421 caggcgaagt ggtttccatc ctaggcccaa gtggtgttgg aaagaccacc ctctttaatc
    12481 taatcgctgg gattttagaa gttcagtcag ggagaattgt ccttgatggt gaagaaaatc
    12541 ccaaggggcg cgtgagttat atgttgcaaa aggatctgct cttggagcac aagacggtgc
    12601 ttggaaatat cattctgccc ctcttgattc aaaaggtgga taaggcagaa gctatttccc
    12661 gagcggataa aattcttgcg accttccagc tgacagctgt aagagacaag tatcctcatg
    12721 aacttagcgg tgggatgcgc cagcgtgtag ccttactccg gacctacctt tttgggcaca
    12781 agctctttct cttagatgag gcctttagcg ccttggatga gatgacaaag atggaactcc
    12841 acgcttggta tcttgagatt cacaagcagt tgcagctaac aaccctgatc atcacgcata
    12901 gtattgagga ggccctcaat ctcagcgacc gcatctatat cttgaaaaat cgccctgggc
    12961 agattgtttc agaaattaaa ctagattggt ctgaagatga ggacaaggaa gtccaaaaga
    13021 ttgcctacaa acgtcaaatc ttggcagaat taggcttaga taagtagaaa aatagggagt
    13081 tggtgaagat tatcctttac cagcgccctt tttcttttaa aaatgagaaa atttcggtat
    13141 aatagtcaaa caaggtcaag gtttaaagag agaggtgggt ttgttatgag atttaaaaat
    13201 acatcggatc atattgaggc ctacatcaag gcgattttag atcaatctgg tatcgtggag
    13261 ttgcaacgga gtcagttggc agataccttt caggttgttc ctagtcagat taactacgtg
    13321 atcaagacac gctttacgga aagtagaggc tacttggttg aaagtaagcg tggtggcgga
    13381 ggctacattc gtataggacg gattgagttt tctagtcatc atgaaatgct ccgggagctg
    13441 ctttactcga ttggtgagcg agtcagtcaa gaaatttatg aggatattct ccagcttttg
    13501 gttgagcagg aattgatgac caagcaggag atgaatttgc tagaatcagt agctttggat
    13561 cgcgttttag gagaagaagc tccagttgtt cgagcaaaca tgctacgtca gatcatacaa
    13621 gaggtagata gaaaagggaa gtaagatgaa ctattcaaaa gcattgaatg aatgtatcga
    13681 aagtgcctac atggttgctg gacattttgg agctcgttat ctagagtctt ggcacttgtt
    13741 gattgccatg tctaatcaca gttatagtgt agcaggggca actttaaatg attatccgta
    13801 tgagatggac cgtttagaag aggtggcttt ggaactgact gaaacggact atagccagga
    13861 tgaaaccttt acggaattgc cgttctcccg tcgtttgcag gttctttttg acgaagcaga
    13921 gtatgtagcg tcagtggtcc atgctaaggt gctagggaca gagcacgtcc tctatgcgat
    13981 tttgcatgat ggcaatgcct tggcgactcg tatcttggag agggctggat tttcttatga
    14041 agacaagaaa gatcaggtca agattgctgc tcttcgtcga aatttagaag aacgggcagg
    14101 ctggactcgt gaagatctca aggctttacg ccaacgccat cgtacagtag ctgacaagca
    14161 aaattctatg gccaatatga tgggcatgcc gcagactcct agtggtggtc tcgaggacta
    14221 tacgcatgat ttgacagagc aagcgcgttc tggcaagtta gaaccagtca tcggtcggga
    14281 caaggaaatc tcacgtatga ttcaaatctt gagccggaag actaagaaca accctgtctt
    14341 ggttggggat gctggtgtcg ggaaaacagc tctggcgctt ggtcttgccc agcgtattgc
    14401 tagtggtgac gtgcctgcgg aaatggctaa gatgcgcgtg ttagaacttg atttgatgaa
    14461 tgtcgttgca gggacacgct tccgtggtga ctttgaagaa cgcatgaata atatcatcaa
    14521 ggatattgaa gaagatggcc aagtcatcct ctttatcgat gaactccaca ccatcatggg
    14581 ttctggtagc ggaattgatt cgactctgga tgcggccaat atcttgaaac cagccttggc
    14641 gcgtggaact ttgagaacgg ttggtgccac cactcaggaa gaatatcaaa aacatatcga
    14701 aaaagatgcg gcactttctc gtcgtttcgc taaagtgacg attgaagaac caagtgtggc
    14761 agatagtatg actattttac aaggtttgaa ggcgacttat gagaaacatc accgtgtaca
    14821 aatcacagat gaagcggttg aaacagcggt taagatggct catcgttatt taaccagtcg
    14881 tcacttgcca gactctgcta tcgatctctt ggatgaggcg gcagcaacag tgcaaaataa
    14941 atcaaagcat gtaaaagcag acgattctga cttgagtcca gctgacaagg ccttgatgga
    15001 tggcaagtgg aaacaggcag cccagctaat cgcaaaagaa gaggaagtgc ctgtctataa
    15061 agacttggtg acagagtctg atattttgac caccttgagt cgcttgtcag gtatccccgt
    15121 tcaaaaactg actcagactg atgccaagaa atacttaaac ttggaagctg aactgcacaa
    15181 acgtgtcatc ggtcaagacc aagctgtttc aagtattagt cgtgccattc gccgcaatca
    15241 gtcaggaatt cgcagtcaca agcgtccgat cggttccttt atgttcctag gacccacagg
    15301 tgtcggtaag accgagttgg ccaaggcttt ggcagaagtt ctctttgatg acgaatcagc
    15361 ccttatccgc tttgatatga gtgagtatat ggagaaattc gcagccagcc gtctaaatgg
    15421 agctcctcca ggctatgtgg gttacgaaga aggtggggag ttgacagaga aggttcgcaa
    15481 taaaccctat tccgttctcc tctttgatga ggtagagaag gcccatccag atatctttaa
    15541 tgttctcttg caggttctgg atgacggtgt cttgacagat agcaagggac gcaaggtcga
    15601 tttttcaaat accattatca ttatgacatc gaacttaggt gcgactgccc ttcgtgatga
    15661 taagactgtt ggttttgggg ctaaggatat tcgttttgac caggaaaata tggaaaaacg
    15721 catgtttgaa gaactgaaaa aagcttatag accggaattc atcaaccgta ttgatgagaa
    15781 ggtggtcttc catagcctat ctagtgacca tatgcaggaa gtggtgaaga ttatggtcaa
    15841 gcctttagtg gcaagtttgg ctgaaaaagg cattgacttg aaattacaag cttcagctct
    15901 gaaattgtta gcaaatcaag gatatgaccc agagatggga gctcgcccac ttcgcagaac
    15961 cctgcaaaca gaagtggagg acaagttggc agaacttctt ctcaagggag atttagtggc
    16021 aggcagcaca cttaagattg gtgtcaaagc aggccagtta aaatttgata ttgcataaaa
    16081 gaataaaagt accagcatct gaccataagt cacagtggag tgaaactcaa tgaaaatcaa
    16141 agagcaaact aggcagctag ccgcaggttg ctcaaaacac tgttttgagg ttgcagatag
    16201 agctgacgtg gtttgaagag atttttgaag agtataagct gcaagatgaa tgattttctt
    16261 gtattgacgt tgttgttgac aaaaagtagc ggataaatga aatccattcc attatcatag
    16321 atgataggct ggtaggaaat tttcaaatag catacagaaa atagatgtat ggagttctgg
    16381 tagtagaaag ggagagagat gaacatttta gttgcagatg acgaggaaat gattagagaa
    16441 ggaattgcag catttctgac agaagagggt tatcatgtca ttatggctaa ggatggacaa
    16501 gaggtcttgg aaaaatttca agatctccct atccatctca tggtactgga tttaatgatg
    16561 cctaggaaga gtggttttga agtgttaaaa gaaatcaatc aaaagcacga tattcctgtc
    16621 atcgtcttga gtgctctggg agatgaaact actcagtcac aggtatttga tctctatgct
    16681 gatgatcatg tgacaaaacc tttttctttg gtactgcttg tcaagcgtat taaggcgctt
    16741 atcagacgtt actacgtcat agaggatctt tggcgatatc aggatgtaac agtggatttt
    16801 acctcttaca aagcacatta taaaaatgaa gaaattgatc tcaaaccaaa ggaattactg
    16861 gtactaaagt gtttgattca gcataaaaat caagttttaa gtagagagca gatattggaa
    16921 gaaatttcaa aagatgtagc tgatttacct tgtgataggg tcgttgatgt ctatattcgt
    16981 actcttcgca aaaaattagc tttagattgt atcgtgactg tgaaaaatgt tgggtataag
    17041 attagcttat gataaaaaat cctaaattat taaccaagtc ttttttaaga agttttgcaa
    17101 ttctaggtgg tgttggtcta gtcattcata tagctattta tttgaccttt cctttttatt
    17161 atattcaact ggagggggaa aagtttaatg agagcgcaag agtgtttacg gagtatttaa
    17221 agactaagac atctgatgaa attccaagct tactccagtc ttattcaaag tccttgacca
    17281 tatctgctca ccttaaaaga gatattgtag ataagcggct ccctcttgtg catgacttgg
    17341 atattaaaga tggaaagcta tcaaattata tcgtgatgtt agatatgtct gttagtacag
    17401 cagatggtaa acaggtaacc gtgcaatttg ttcacggggt ggatgtctac aaagaagcaa
    17461 agaatatttt gcttttgtat ctcccatata catttttggt tacaattgct ttttcctttg
    17521 ttttttctta tttttatact aaacgcttgc tcaatcctct tttttacatt tcagaagtga
    17581 ctagtaaaat gcaagatttg gatgacaata ttcgttttga tgaaagtagg aaagatgaag
    17641 ttggtgaagt tggaaaacag attaatggta tgtatgagca cttgttgaag gttattcatg
    17701 agttggaaag tcgtaatgag caaattgtaa aattgcaaaa tcaaaaggtt tcctttgtcc
    17761 gtggagcatc acatgagttg aaaacccctt tagccagtct tagaattatc ctagagaata
    17821 tgcagcataa tattggagat tacaaagatc atccaaaata tattgcaaag agtataaata
    17881 agattgacca gatgagccgc ttattggaag aagtactgga gtcttctaaa ttccaagagt
    17941 ggacagagtg tcgtgagacc ttgactgtta agccagtttt agtagatatt ttatcacgtt
    18001 atcaagaatt agctcattca ataggtgtta caattgaaaa tcaattgaca gatgctacca
    18061 gggtcgtcat gagtcttagg gcattggata aggttttgac aaacctgatt agtaatgcaa
    18121 ttaaatattc agataaaaat gggcgcgtaa tcatatccga gcaagatggc tatctctcta
    18181 tcaaaaatac atgtgcgcct ctaagtgatc aagaactaga acatttattt gatatattct
    18241 atcattctca aatcgtgaca gataaggatg aaagttctgg tttgggtctt tacattgtga
    18301 ataatatttt agaaagctat caaatggact atagttttct cccttatgaa cacggtatgg
    18361 aatttaagat tagcttgtag acagattagt tttttattaa agttcatata gggttaacat
    18421 aagtgtgtta ttctttgtgt agataaaaga aaggatacta atatggtatt agcgattatt
    18481 ttagtaacat tctttattcg attgattttt ttaaagcgtt cgatagagaa tgagaaacga
    18541 atccttagca atggcgggaa agaatttgga gttgagaata caaaacgatt aactatggct
    18601 catattgttt tttatctctc ttgcttggtt gaggcaatgg tgcacaagac aatttttgat
    18661 ggcatgggca tggttggttt ggtcttgctt attttttcta tgctgatgtt gatgttggtg
    18721 attcacttgt tgggagatat ttggacagtg aagcttatgc ttgtcaataa tcacaaatat
    18781 gtagatcata tcttgtttag gacagtaaaa caccctaatt actttttaaa tattttacct
    18841 gagttgattg gcttgacctt gttgagtcat gcttatgtga cttttgtttt agtttttcca
    18901 gtttatgcag ttattttgta tcgacgaata gctgaagagg aaaagctatt acatgaagtt
    18961 ataatcccaa atggaagcat aaagagataa atacaaaatt cgatttatat acagttcata
    19021 ttgaagtgat atagtaaggt taaagaaaaa atatagaagg aaataaacat gtttgcatca
    19081 aaaagcgaaa gaaaagtaca ttattcaatt cgtaaattta gtattggagt agctagtgta
    19141 gtagttgcta gcttgttctt aggaggagta gtccatgcag aaggggttag aagtgagaat
    19201 aaccccacgg ttacatctag tgggcaagat atatcgaaga agtatgctga tgaagtcaag
    19261 tcacatctag aaaaaatatt gagtgagatc caaacaaatt tagatagaag taaacatatc
    19321 aaaactgtaa atctaattaa caaattgcaa gacattaaga gaacgtattt gtatgaatta
    19381 aatgttttag aagataagtc gaaagctgag ttgccgtcaa aaataaaagc agagttagac
    19441 gcagcttttg agcagtttaa aaaagataca ttaccaacag aaccaggaaa aaaggtagca
    19501 gaagctaaga agaaggttga agaagctgag aaaaaagcca aggctcaaaa agaagaagat
    19561 taccgtaact acccaaccat tacttacaaa acgcttgaac ttgaaattgc tgagtccgat
    19621 gtgaaagtta aagaagcgga gcttgaacta gtaaaaaagg aagctgacga atctcgaaac
    19681 gagggcacaa ttaaccaagc aaaagcgaaa gttgagagtg aacaagctga ggctacaagg
    19741 ttaaaaaaaa tcaagacaga tcgtgaaaaa gcagaagaag aagctaaacg aagagcagat
    19801 gctaaagagc aagatgaatc aaagaggcga aagagtcggg taaaacgagg agatcttgga
    19861 gagccagcaa cacctgataa aaaagaaaat gatgcgaagt cttcagattc tagcgtaggt
    19921 gaagaaactc ttccaagccc atccctgaaa ccaggaaaaa aggtagcaga agctgagaag
    19981 aaggttgaag aagctgagaa aaaagccaag gatcaaaaag aagaagatca ccgtaactac
    20041 ccaaccatta cttacaaaac gcttgaactt gaaattgctg agtccgatgt ggaagttaaa
    20101 aaagcggagc ttgaactagt aaaagaggaa gctaagggat ctcgaaacga ggaaaaagtt
    20161 aagcaagcaa aagcggaagt tgagagtaaa aaagctgagg ctacaaggtt agaaaaaatc
    20221 aagacagatc gtaaaaaagc agaagaagaa gctaaacgaa aagcagcaga agaagataaa
    20281 gttaaagaaa aaccagctga acaaccacaa ccagcgccgg ctcctcaacc agaaaaacca
    20341 gctccagctc caaaaccaga gaatccagct gaacaaccaa aagcagaaaa accagctgat
    20401 caacaagctg aagaagacta tgctcgtaga tcagaagaag aatataatcg cttgactcaa
    20461 cagcaaccgc caaaaactga aaaaccagca caaccatcta ctccaaaaac aggctggaaa
    20521 caagaaaacg gtatgtggta cttctacaat actgatggtt caatggcgac aggatggctc
    20581 caatacaatg gttcatggta ctacctcaac gctaatggtg atatggcgac aggatggttc
    20641 caatacaatg gttcatggta ctacctcaac gctaatggtt caatggcaac aggatggctc
    20701 caatacaatg gttcatggta ctacctcaac gctaacggtt caatggcaac ag
//
LOCUS       DBERYS010000036        20106 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.036, whole genome shotgun sequence.
ACCESSION   DBERYS010000036 DBERYS010000000
VERSION     DBERYS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.036"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..435)
                     /locus_tag="ACL8LA_001481"
     CDS             complement(<1..435)
                     /locus_tag="ACL8LA_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1813226.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYNPLLIKEFAKSLSLHKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKNSLI"
     gene            691..1551
                     /gene="speE"
                     /locus_tag="ACL8LA_001482"
     CDS             691..1551
                     /gene="speE"
                     /locus_tag="ACL8LA_001482"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGS1813227.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            1548..2807
                     /locus_tag="ACL8LA_001483"
     CDS             1548..2807
                     /locus_tag="ACL8LA_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGS1813228.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            2807..3934
                     /gene="nspC"
                     /locus_tag="ACL8LA_001484"
     CDS             2807..3934
                     /gene="nspC"
                     /locus_tag="ACL8LA_001484"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGS1813229.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            3931..5016
                     /gene="aguA"
                     /locus_tag="ACL8LA_001485"
     CDS             3931..5016
                     /gene="aguA"
                     /locus_tag="ACL8LA_001485"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGS1813230.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            5026..5901
                     /gene="aguB"
                     /locus_tag="ACL8LA_001486"
     CDS             5026..5901
                     /gene="aguB"
                     /locus_tag="ACL8LA_001486"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGS1813231.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(6082..6891)
                     /locus_tag="ACL8LA_001487"
     CDS             complement(6082..6891)
                     /locus_tag="ACL8LA_001487"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1813232.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            7163..7384
                     /locus_tag="ACL8LA_001488"
     CDS             7163..7384
                     /locus_tag="ACL8LA_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGS1813233.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            7438..8109
                     /locus_tag="ACL8LA_001489"
     CDS             7438..8109
                     /locus_tag="ACL8LA_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1813234.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8161..8358)
                     /locus_tag="ACL8LA_001490"
     CDS             complement(8161..8358)
                     /locus_tag="ACL8LA_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1813235.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            8351..9259
                     /locus_tag="ACL8LA_001491"
     CDS             8351..9259
                     /locus_tag="ACL8LA_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS1813236.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            9256..9717
                     /gene="lspA"
                     /locus_tag="ACL8LA_001492"
     CDS             9256..9717
                     /gene="lspA"
                     /locus_tag="ACL8LA_001492"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGS1813237.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            9707..10594
                     /locus_tag="ACL8LA_001493"
     CDS             9707..10594
                     /locus_tag="ACL8LA_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1813238.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            10597..12480
                     /gene="cbpE"
                     /locus_tag="ACL8LA_001494"
                     /gene_synonym="pce"
     CDS             10597..12480
                     /gene="cbpE"
                     /locus_tag="ACL8LA_001494"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGS1813239.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVARHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            12581..13690
                     /gene="proB"
                     /locus_tag="ACL8LA_001495"
     CDS             12581..13690
                     /gene="proB"
                     /locus_tag="ACL8LA_001495"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGS1813240.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            13700..14962
                     /locus_tag="ACL8LA_001496"
     CDS             13700..14962
                     /locus_tag="ACL8LA_001496"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGS1813241.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            14966..15763
                     /gene="proC"
                     /locus_tag="ACL8LA_001497"
     CDS             14966..15763
                     /gene="proC"
                     /locus_tag="ACL8LA_001497"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGS1813242.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            15879..16517
                     /gene="tmk"
                     /locus_tag="ACL8LA_001498"
     CDS             15879..16517
                     /gene="tmk"
                     /locus_tag="ACL8LA_001498"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGS1813243.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK"
     gene            16514..17404
                     /locus_tag="ACL8LA_001499"
     CDS             16514..17404
                     /locus_tag="ACL8LA_001499"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGS1813244.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            17444..17761
                     /gene="yabA"
                     /locus_tag="ACL8LA_001500"
     CDS             17444..17761
                     /gene="yabA"
                     /locus_tag="ACL8LA_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGS1813245.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            17764..18633
                     /gene="rsmI"
                     /locus_tag="ACL8LA_001501"
     CDS             17764..18633
                     /gene="rsmI"
                     /locus_tag="ACL8LA_001501"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGS1813246.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            18695..18823
                     /locus_tag="ACL8LA_001502"
                     /pseudo
     CDS             18695..18823
                     /locus_tag="ACL8LA_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            18860..19378
                     /locus_tag="ACL8LA_001503"
     CDS             18860..19378
                     /locus_tag="ACL8LA_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HGS1813247.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            19487..20106
                     /locus_tag="ACL8LA_001504"
                     /pseudo
     CDS             19487..20106
                     /locus_tag="ACL8LA_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT         6072 a         3547 c         4641 g         5846 t
ORIGIN      
        1 aatcagactg tttttcttga gacgatgaat atgtctagcc agtattttta gttctacttg
       61 ccgattatcg tgttgaaatt gttcacgatt ggggtcagaa agaagtttaa gagcgatgcc
      121 atgagcgtct ttcttatccg ttttagtctt gtgaagtgat aatgatttgg caaattcctt
      181 gatgagcaaa ggattgtagg tgtaaacttt atatccttgt tcatgcagga agttcagtag
      241 attaaaggca taatgtccgg tattttcaag agcgatgaga cagtcttggt tgagttgtcg
      301 aagagacaga tctaagagtt caaaaccagc tttattattt gaaaaagtga gtggtttaag
      361 aacagttttt cctggaacat tcaaggctgt aacatcgtgt ttatttttag cgacatcaat
      421 gcccacataa agcatgggag tacctccaga tgtagtattt caagtctact tggttatcca
      481 cgaatttttt gccttgttac cttagacgag ataaaacgtc tatgcgttat caaactcatt
      541 accaattgaa acaaaaaact gtggttagag cctttcggaa atcgtcaagc gattggaggg
      601 aataaactaa tccacagtgg cttattccaa gtataccact tgggctttgg cagtagctaa
      661 ctgcgctaaa tataatataa ggagaaatag atggatttat ggttttctga agttcatact
      721 ccagatgtca aattgtctct gagaacagcc aagcaacttt acgctggaaa aagtgaatgg
      781 caggatatcg aagtcttgga tacgccagct tttgggaaaa tactgatttt aaatggccat
      841 gtcttgttct cagatgcgga tgatttcgtc tacaatgaaa tgaccgttca cgttcccatg
      901 gctgtccacc caaatccaaa gaaagtattg gttattgggg gtggtgacgg cggtgttgcc
      961 caagtattaa cactctatcc tgaactggaa caaatcgata tcgtggaacc ggatgagatg
     1021 ttggtcgagg tctgtcgtga gtatttccca gactttgctg cagggctaga tgatcctcgt
     1081 gttaccattt actaccaaaa tgggctacgc tttttgcgaa actgcgaaga tgattacgat
     1141 attatcatca acgatgcgac agatccattt ggccatacgg aaggactctt taccaaggaa
     1201 ttctacggca atagttatcg agctctgaag gaagacggca tcatgattta ccagcatggg
     1261 agtcccttct ttgacgagga tgagtcggcc tgccgaagca tgcaccgcaa ggtcaatcaa
     1321 gcctttccaa tcagtcgggt ttatcaggcc catattccaa ctagcccagc tggctattgg
     1381 ttgtttggat ttgcatcgaa aaaataccac cctgtcaaag attttgacaa ggaaggttgg
     1441 aaaaaacgcc agcttttcac agaatactac actgcaaact tacacgtggg atcctttatg
     1501 ttgcccaagt atgttgagga cattttagaa gaagaggaag gaaaaaaatg agtcgtttac
     1561 tagttattgg ttgtgggggc gttgcccaag ttgctatttc aaagatttgt caagatagcg
     1621 aaacatttac agagattatg attgctagcc gtaccaagtc aaaatgcgat gacttgaaag
     1681 cgaagctaga aggcaaaaca agtactaaaa ttgaaactgc agcacttgat gctgacaagg
     1741 ttgaagaagt gattgccctg attgaaagct acaaaccaga agctgttttg aatgtagctc
     1801 tgccttatca agatttaacc attatggatg cttgtttggc aacaggtgtt cactatatcg
     1861 atacagccaa ctacgaagca gaagacacag aagaccctga gtggcgtgct atctacgaaa
     1921 aacgttgtaa ggaacttggt tttacagcct actttgacta ctcatggcag tgggcttatc
     1981 aagagaaatt caaagaagca ggcttgactg ctcttcttgg ttctggtttt gacccaggtg
     2041 taactagtgt cttttcagct tatgccctca aacactattt tgatgaaatc cattatatcg
     2101 acattttaga ctgtaatggc ggtgaccacg gttatccatt tgcaaccaac tttaatccag
     2161 aaattaatct ccgtgaggtt tctgcgccag gttcttactg ggaagatggg aaatgggtcg
     2221 aagtcgaagc tatgtctatc aagcgtgagt atgatttccc tcaagttgga caaaaagata
     2281 tgtatctcct tcaccatgaa gaaatcgaat cattggccaa gaacattcca ggtgtcaaac
     2341 gcattcgttt ctttatgact tttggtcaat cttacttgac gcacatgaaa tgtcttgaaa
     2401 atgttggact ccttcgtacg gataccatta actttaacgg ccaagaaatt gttccaattc
     2461 aatttttgaa agccttgctt ccagatcctg ctagccttgg cccacgtact gtaggtaaaa
     2521 ccaatattgg atgtatcttt acaggtgtca aagatggcgt tgaaaagact atctacatct
     2581 acaatgtttg cgaccatcaa gagtgctatg cagaagtcgg gtcacaagca atttcataca
     2641 cgacaggcgt tccagccatg attgggacaa aattagtcat gaacggaact tggaaacaag
     2701 ctggagtgta taaccttgag gagttagatc cagatccatt catggaagct ttgaatgagt
     2761 atggtttgcc atgggttgtg gttgaaaatc cacaaatggt ggactaatga agttagaaca
     2821 agtaccaaca ccagcctatg ttattgactt ggccaagtta gaagctaatt gccgcattct
     2881 acaatatgta caagaagagg ccggttgcaa ggtcttgctt gcccagaagg catattccct
     2941 ctacaaaact tatcccttga ttagccagta tctatcaggt acgacagcta gtggactcta
     3001 tgaggccaaa ttggcaaggg aagaatttcc tggtgaagtc catgtttttg caccagcttt
     3061 caaggatgag gacatggagg aattgcttgg gataacagac catatcgtct ttaactcaga
     3121 gagacagttg cgtaagcatg gggctcgttg tcgagaggct ggtgtcagtg ttggtttgcg
     3181 cctcaaccct cagtgttcaa ctcaaggaga tcacgcgctc tatgaccctt gtgcacctgg
     3241 ttctcgcttt ggagttacta tagacaagat tccgagtgat ttgctagatt tggttgacgg
     3301 acttcatttt catacccttt gcgagcaggg agcagatgat ttacaaacaa ctttgaaagc
     3361 agtagaagaa cagtttggtc cctacttaca tgaggtaaaa tggctcaata tgggtggtgg
     3421 tcatcatatt acaagagaag gttacgatgt ggatttgctg atttcagaaa tcaagcgtat
     3481 ccgaaaaact tacaatcttg aaatctatat cgagcctggt gaagccattg cgcttaatgc
     3541 gggttattta gcaactgagg tattagatat tgtagaaaac ggtatggaaa tcttggtttt
     3601 agacgcctct gcgacctgcc atatgcctga tgtacttgag atgccctatc gtccaccttt
     3661 gagaaatggc tttgagtcac aggaaaaagc ccatacctac agactttctt ctaatacctg
     3721 tctgactggc gatgtgattg gtgattatag ttttgaaaat ccagtccaaa tcggagacag
     3781 actttatttt caagacatgg ccatttattc ttttgtcaaa aataatacct ttaatggtat
     3841 tggattgcca agtctctatc tcatggacga acagggagac tgtagcttac tcaaagcttt
     3901 tggctatcaa gactttaaag ggagattatc atgatggaca gtccaaaaaa attaggctat
     3961 cacatgccag cagagtacga accccatcat ggtaccctca tgatatggcc gactcgacca
     4021 ggatcatggc cttttcaagg aaaggctgct aaaagagcat ttactcagat catcgagacc
     4081 atagcagaag gggaaagagt ctatcttttg gtggagcagg cctatctatc tgaagcccaa
     4141 tcctatcttg gagacaaggt tgtttattta gacattccca ccaatgatgc ctgggcgcgt
     4201 gatactggcc caaccattct cgtcaatgat aaaggtaaga aattagccgt ggattgggcc
     4261 tttaatgctt ggggaggcac ctatgatggt ctttatcaag attatgaaga ggatgaccaa
     4321 gtagccagtc gttttgctga ggccttggaa aggcctgtct atgatgctaa accttttgta
     4381 ctggaaggag gcgcaatcca tagcgatggt caaggaacta ttctcgtaac tgaaagttgc
     4441 ttgcttagtc ctggtcgcaa tcctaacctg actaaagagg agattgaaaa cacattatta
     4501 gaaagtcttg gtgctgaaaa agttatttgg cttccttatg gtatttatca ggatgaaacc
     4561 aatgaacacg tcgataatgt tgttgccttt gttggtcctg ctgaggttgt tttggcttgg
     4621 acagatgacg aaaatgatcc ccagtatgcc atgtcaaaag cagatctcga actcttagaa
     4681 caggaaacag atgcaaaagg ttgtcacttc accattcata aattgcctat ccctgcagtt
     4741 cgacaagttg tgacagaaga agatttgcca ggctacatct atgaagaagg agaagaagag
     4801 cgatacgcag gtgaacgact agcagcttcc tacgtaaact tttatatcgc caacaaggct
     4861 gtcttggttc cacagtttga ggatgtaaac gaccaagtgg ccttagatat cctcagcaag
     4921 tgtttcccag accgtaaagt tgtcggaata ccagccagag atattctctt aggtggtggc
     4981 aatatccact gtatcaccca acaaattcca gaataggaga aaaagatgag aaatgtaaga
     5041 gttgcaacca ttcagatgca atgcgctaag gatgtggcaa caaatatcca aaccgcagag
     5101 cgtttagtac gtcaggctgc tgagcaagga gcccaaatta ttctcttgcc cgagttgttt
     5161 gaacatccct atttctgtca ggaacgtcag tatgactact accagtatgc ccaatctgta
     5221 gcggaaaata ctgccattca gcattttaag gtgattgcca aggaactaca agttgtttta
     5281 ccaatcagtt tctatgaaaa agatggtaat gtcttgtata actctattgc cgtcattgat
     5341 gcagatgggg gagtgctggg cgtttatcga aagacccata taccagatga ccattattat
     5401 caagaaaaat tctatttcac gcctggtaac actggtttca aggtctggaa tactcgctat
     5461 gctaagattg gtatcggtat ctgttgggat caatggttcc ctgaaacagc ccgctgtctt
     5521 gcattgaatg gtgctgaatt gctcttttat cctacagcta tcggttcaga gccaattttg
     5581 gatacagata gttgtggtca ctggcaacgt actatgcaag ggcacgcagc agcgaatatt
     5641 gttccagtca tcgcagccaa tcgttatggt ttagaggagg ttactcctag tgaggaaaat
     5701 ggcggacaga gttccagtct tgacttctac ggttcctcct ttatgacgga tgaaacagga
     5761 gctattctag aacgagctga aagacaagaa gaagctgttc tgttagctac ttatgaccta
     5821 gacaagggag caagtgaacg tctaaactgg ggcttgtttc gagatagaag accagaaatg
     5881 tatagacaaa ttacagatta gtgtgggaga aatgagagat tcattctgct agactaactt
     5941 cttattagca actataagat actatggcat ctagtaaatc gatttttatg attcgctatt
     6001 cttgtctatt gattagtccg tattttaaaa tattagcaaa aaagcaaata gcagtaactt
     6061 ctgtctattt gcttttcttt tttatagaat atatttctca atagcacgcg caacgccgtc
     6121 ttcttcgttg cttgaggtaa cggcatccgc aagagatttg acataatcgc tggcatttcc
     6181 cattgcaatc ccaagccctg caaactggag catttcgata tcgttattag catcgcccat
     6241 ggccataatc tctgaggaat caatcttcaa aatctcagct agtcgtgaaa gagcagtagc
     6301 ctttgtcgtt ccaagcggca ttgcttcata aatgacaggc tgcgaacgaa ctccactgaa
     6361 tcgttggcaa agctcttcag caaaacgctg ctcaaaatcg tctgtttgtt cttttgttcc
     6421 taaaaacata ccttggaaca tccggaactt tccactagtc gcttcttcaa gagaaatttc
     6481 agtcaggtct gaaaatacta gtttagcatc attttcaata acttgattgg gtttgccacc
     6541 gagaacaaaa taatgtgact cgtcaaaaag tgtcaactga acatcactct tttcagcaag
     6601 gtcatagagg tattcgatgt cagctggact cagttctttc cagtcaacta gactccaatc
     6661 actggtctgg tgagttgaac aaccgttgtt aacaataata tattcgttct ggaggtcaag
     6721 ctccagtttt ttgtagtagg ggaggacacc gaaaaggggg cgacccgtac agagaaccag
     6781 tttgacacct ttttcaatgg ctttgtgaat agcagtaatg tgagcttgtg ggatttcctt
     6841 ggcttcattg aggagggtgc cgtccatatc caaggctagt agtttaatca taggtcttcc
     6901 ttctttatct tttgctatta ttatagcata ttttggagaa gaaattgata gaaagcttga
     6961 gactaattga ttttatagtt taagatgttt tgatgacaat tcatgatttg aagaggatat
     7021 ttggcaaaga tatgctatac tatgtttgtc aatgttgcaa ctagacaaat taaaaaacca
     7081 acttaatata atagtttttt tgtaagtagg tatgagtatc agattactca actaatctga
     7141 agaataatgg aggaaatata tcatgatttt aatgacaaaa aatataaatc taacaaatga
     7201 agaattagag ctgatacaag gtggagcaga ttcatatggt aaaaatccta atggtaggta
     7261 cgattgggaa atagaaccag tattaactct gccggttcat ggattttgtt ccagaggcac
     7321 ctatgattta ggatatattg gaggaggcaa tcatctttgc aaaggaagtg ctgcgagatt
     7381 ttaagtaaaa tttattagta atatgaagaa acaagtggag aaagcagaga atttaatatg
     7441 aaaaaacggg ctattcaaat tttactagca ttgtccttaa ttttttacaa atcaacttgg
     7501 ttttggagag ttttcaatca cctcgcaaag ccctatctac cagcaagtcg tgaatttttt
     7561 cagattctgc ttttgatgga gagcggagtt cttttcttag cggtcatcta tctactggtt
     7621 tttgcaggaa agaaaatttt tcatttcaag tggcagctga ggtacttcat ctacctttta
     7681 ctgggctaca tcatttcata tatgtctgac ttcctctttt cgtatttcat atccctgtct
     7741 tcaaatcaga tttctttgaa tgaaacgata gaaatgatgg ggagacagga gttcccttat
     7801 ttcttgctca tcgtttgctt catcgcccct attgctgagg aattgattta tcgaggtgtg
     7861 cttatgacaa cctttttcaa aaactcacct tggtacggag atgttttgct ttctgctatt
     7921 attttcggtt atattcatat caattttgct ttaactcctc ttgctttttt catttatgct
     7981 agtggaggtc tgattttagc tctattgtat cgcatgacta aaaatctcta ctatccaata
     8041 ctagttcata ttctcatcaa tatcactgcc ttctgggatg tttggttact cctattttca
     8101 ggaagttagc ttactaaaaa aatgtcggaa ttttccggca ttttcttttt tcacaaatag
     8161 tcaacgtttt tctttccgat actgaagtgg tgtgtagcca cttatttttt tgaattgatt
     8221 ttgaaaataa gattggcgtg agaaaggcag atagtgaaga tagttaagaa gaataggatg
     8281 ttcttttttc ctttttggaa aacttctaaa atatggtata atgaaaagat aaagaagttg
     8341 ggggtagaag atgaacattc aacaattacg ctatgttgtg gctattgcca atagtggtac
     8401 ttttcgtgaa gctgctgaaa agatgtatgt tagtcagccg agtctgtcta tttctgttcg
     8461 tgatttggaa aaagagttgg gctttaagat tttccgtcgg accagctcag ggactttctt
     8521 gacccgtcgt gggatggaat tttatgaaaa agcgcaagaa ttggttaaag gatttgatat
     8581 ttttcaaaat cagtatgcca atcctgaaga agaaaaagat gaattttccg ttgctagcca
     8641 gcactatgac ttcttaccac caactattac ggccttttca gagcgctatc ctgactataa
     8701 gaacttccgt atttttgaat caactactgt tcaaatatta gatgaagtgg cgcaagggca
     8761 tagtgagatt gggattatct acctcaacaa tcaaaataaa aaggggatta tgcaacgggt
     8821 tgaaaagtta ggtctggagg tcatcgaatt gattcctttc catacccata tttatctctg
     8881 tgagggtcat cctttagccc agaaagagga attagtcatg gaggatttag cggatttacc
     8941 aacggttcgt ttcactcaag agaaagacga gtacctttat tattcagaga actttgtcga
     9001 taccagcgct agctcacaga tgtttaatgt gacagaccgt gccaccttga atggtatttt
     9061 ggagcggacg gacgcctatg cgacaggttc tggattttta gatagtgaca gtgttaatgg
     9121 cattacagtt attcgtctca aggataacct agataaccgc atggtctatg ttaaacgtga
     9181 agaagtggag cttagtcaag ctgggactct cttcgtagaa gtcatgcaag aatattttga
     9241 tcaaaagagg aaatcatgaa aaaaagagca atagtggcag tcattgtact gcttttaatt
     9301 gggctggatc agttggtcaa atcttatatc gtccagcaga ttccactggg tgaagtgcgc
     9361 tcctggatcc ccaatttcgt tagcttgacc tacctgcaaa atcgaggtgc agccttttct
     9421 atcttacaag atcagcagtt gttattcgct gtcattactc tggttgtcgt gataggtgcc
     9481 atttggtatt tacataaaca catggaggac tcattctgga tggtcttggg tttgactcta
     9541 ataatcgcgg gtggtcttgg aaactttatt gacagggtca gtcagggctt tgttgtggat
     9601 atgttccacc ttgactttat caactttgca attttcaatg tggcagatag ctatctgacg
     9661 gttggagtga ttattttatt gattgcaatg ctaaaagagg aaataaatgg aaattaaaat
     9721 tgaaactggt ggtctgcgtt tggataaggc tttgtcagat ttgtcagaat tatcacgtag
     9781 tctcgcgaat gaacaaatta aatcaggcca ggtcttggtc aatggtcaag tcaagaaagc
     9841 taaatacaca gtccaagagg gtgatgtcgt cacttaccat gtgccagaac cagaggtatt
     9901 agagtatgtg gctgaggatc ttccgctaga aatagtctac caagatgagg atgtggctgt
     9961 cgttaacaaa cctcagggaa tggttgtgca cccgagtgct ggtcatacca gtggaaccct
    10021 agtaaatgcc ctcatgtatc atattaagga cttgtcgggt atcaatgggg ttctgcgtcc
    10081 agggattgtt caccgtattg ataaggatac gtcaggtctt ctcatgattg ctaaaaacga
    10141 tgatgcgcat ctagtacttg cccaagaact caaagataaa aagtctctcc gcaaatattg
    10201 ggcgattgtt catggaaatc tgcctaatga tcgtggtgta attgaagcgc cgattggccg
    10261 gagtgaaaaa gaccgtaaga aacaggctgt aactgctaaa gggaagcctg cagtgacgcg
    10321 ttttcacgtc ttggaacgct ttggcgatta tagcttagta gagttgcaac tggagacagg
    10381 gcgcactcat caaatccgtg tccacatggc ttatatcggc cattcagtcg ctggtgatga
    10441 ggtctatggt cctcgcaaga ctttgaaagg acatggacaa tttcttcatg ccaagacttt
    10501 aggttttact catccgagaa caggtaagac cttggaattt aaagcagata tcccagagat
    10561 ttttaaggaa accttggaga gattgagaaa gtaagaatga aaaagaaatt aactagttta
    10621 gcacttgtag gcgctttttt aggtttgtca tggtatggga atgttcaggc tcaagaaagt
    10681 tcaggaaata aaatccactt tatcaatgtt caagaaggtg gcagtgatgc gattattctt
    10741 gaaagcaatg gacattttgc catggtggat acaggagaag attatgattt cccagatgga
    10801 agtgattctc gctatccatg gagagaagga attgaaacgt cttataagca tgttctaaca
    10861 gaccgtgtct ttcgtcgttt gaaggaattg ggtgtccaaa aacttgattt tattttggtg
    10921 acccataccc acagtgatca tattggaaat gttgatgaat tactgtctac ctatccagtt
    10981 gaccgagtct atcttaagaa atatagtgat agtcgtatta ctaattctga acgtctatgg
    11041 gataatctgt atggctatga taaggtttta cagactgctg cagaaaaagg tgtttcagtt
    11101 attcaaaata tcacacaagg ggatgctcat tttcagtttg gggacatgga tattcagctc
    11161 tataattatg aaaatgaaac tgattcatcg ggtgaattaa agaaaatttg ggatgacaat
    11221 tccaattcct tgattagcgt ggtgaaagtc aatggcaaga aaatttacct tgggggcgat
    11281 ttagataatg ttcatggagc agaagacaag tatggtcctc tcattggaaa agttgatttg
    11341 atgaagttta atcatcacca tgataccaac aaatcaaata ccaaggattt cattaaaaat
    11401 ttgagtccga gtttgattgt tcaaacttcg gatagtctac cttggaaaaa tggtgttgat
    11461 agtgagtatg ttaattggct caaagaacga ggaattgaga gaatcaatgc agccagcaaa
    11521 gactatgatg caacagtttt tgatattcga caagacggtt tagtaaacat ttcaacttcc
    11581 tacaagccga ttccaagttt tcaagctggt tggcataaga gttcatatgg aaactggtgg
    11641 tatcaagcgc ctgattctac aggagaatat gctgtcggtt ggaatgaaat taagggtgaa
    11701 tggtattact ttaaccaaac gggtatctta ttacagaatc aatggaaaaa atggaacaat
    11761 cgttggttct atttgacaga ctctggtgtt gctgctaaaa attggaagaa aatcgctgga
    11821 atctggtatt attttaacaa agaaaaccag atggaaattg gttggattca agataaagag
    11881 cagtggtatt atttggatgt tgatggctct atgaagacag gttggcttca atataagggg
    11941 caatggtatt actttgctcc atcaggggaa atgaaaacgg gctgggtaaa agataaagaa
    12001 gcctggtact atatggattc tactggtatc atgaagacag gtgagataga agttgctaga
    12061 catcattact atctagaaga atcaggagct atgaagcaag gctggcttaa aaaggcaaat
    12121 gattggtatt tctacaagac agatggttca cgagctatag gttggattaa agacaaggat
    12181 aaatggtact tcttgaaaga aaatggtcaa ttacttgtga atggtaagac accagaaggc
    12241 tatactgtgg attcaagtgg tgcctggtta gtggatgttc cgatcgagaa atctgctaca
    12301 attaaaacta caagtcattc agaaataaaa gaatccaaag aagtagtgaa aaaggatctt
    12361 gaaaataaag aaacgagtca acatgaaagt gttacaaatt cttcaactag tcaagatttg
    12421 acatcctcaa cttcacaaag ctctgaaacg agtgtaaaca aatcggaatc agaacagtag
    12481 tagaaaagaa ggttttaggg ccttcttttt cctatcaact cttttctatt tcctgttatt
    12541 catgttataa tggataaata tgaataatcg gagtgagact atgaaataca aacggattgt
    12601 ctttaaggtg ggtacttctt ctctgacaaa tgaggatgga agtttatcac gtagtaaggt
    12661 aaaggatatt acccagcagt tggctatgct gcacgaggct ggtcatgagt tgattttggt
    12721 gtcttcaggt gccattgcgg ctggttttgg agccttagga tttaaaaagc gtccgactaa
    12781 aattgctgat aaacaggctt cagcagcggt agggcaggga cttttgttgg aagaatatac
    12841 gaccaatctt ctcttgcgtc aaatcgtttc tgcacaaatc ttgctgaccc aagatgactt
    12901 tgtggataag cgtcgttata aaaatgccca tcaggctttg tcggttttgc tcaaccgtgg
    12961 ggcaattcct atcatcaatg agaatgatag tgtcgttatt gatgagctca aggttgggga
    13021 caatgacact ctaagtgctc aagtagcggc gatggtccaa gcagaccttt tagttttctt
    13081 gacagatgtg gacggtctct atactggaaa tcctaattca gatccaagag ccaaacgctt
    13141 ggagagaatc gagaccatca atcgtgagat tattgatatg gctggtggag ctggttcgtc
    13201 aaacggaact gggggtatgt taaccaaaat caaggctgca actatcgcga cggaatcagg
    13261 agttcctgtt tatatctgct catccttgaa atcagattcc atgattgagg cggcagagga
    13321 gaccgaggat ggttcttact ttgttgctca agagaagggg cttcgtaccc agaaacaatg
    13381 gcttgccttc tatgctcaga gtcaaggttc tatttgggtt gataaagggg ctgcggaagc
    13441 tctctctcaa catggaaaga gtcttctctt atctggtatc gttgaagcag aaggagtctt
    13501 ttcttacggt gatatcgtga cagtatttga taaggaaagt ggaaaatcac ttggaaaagg
    13561 acgcgtgcaa tttggagcat ctgctttgga ggatatgttg cgttctcaaa aagccaaggg
    13621 tgtcttgatt taccgtgacg actggatttc cattactcct gaaatccaac tactttttac
    13681 agaattttag aggtaaacta tggtgagtag acaagaacaa tttgaacagg tacaggctgt
    13741 taaaaaatcg attaacacag ctagtgaaga agtgaaaaac caagccttgc tagccatggc
    13801 tgatcactta gtggctgcta ctgaggaaat tttagcggct aatgccctcg atatggcagc
    13861 ggctaagggg aaaatctcag atgtgatgtt ggatcgtctt tatttggatg cagatcgtat
    13921 agaagcgatg gcaagaggaa ttcgtgaagt ggttgcctta ccagatccaa tcggtgaagt
    13981 tttagaaaca agtcagcttg aaaatggttt ggttatcaca aaaaaacgtg tagctatggg
    14041 tgtcatcggt attatctatg aaagccgtcc aaatgtgacg tctgatgcgg ctgctttgac
    14101 tcttaagagt ggaaatgcgg ttgttcttcg tagtggtaag gatgcctatc aaacaaccca
    14161 tgccattgtc acagccttga agaagggctt ggagacgact actattcatc caaatgtgat
    14221 tcaactggtg gaggatacta gccgtgaaag tagttatgct atgatgaagg ccaagggcta
    14281 tctagacctt ctcattcctc gtggaggagc tggcttgatc aatgcagtgg ttgagaatgc
    14341 gattgtacct gttatcgaga cagggactgg gattgtccat gtctatgtgg ataaggatgc
    14401 agacgaagac aaggcgctgt ctatcatcaa caatgctaaa accagtcgtc cttctgtttg
    14461 taatgccatg gaggttctgc tggttcatga aaacaaggca gcaagcatcc ttcctcgctt
    14521 ggatcaaatg ctggttgcag atcgaaaaga agctgggttg gaaccaattc aattccgcct
    14581 agatagcaaa gcaagccagt ttgtttcagg tcaagctgct caagcacaag actttgatac
    14641 cgagttttta gactatattc tagctgttaa ggttgtgagc agtttagaag aagcggttgc
    14701 gcatattgaa tcccacagta cccatcattc ggatgctatt gtgacggaaa atgctgaagc
    14761 tgcagcatac tttacagatc aagtggactc tgcagcggtg tatgttaatg cctcaactcg
    14821 tttcacagat ggaggacaat ttggtcttgg ttgtgaaatg gggatttcta ctcagaaatt
    14881 gcacgcgcgt ggtccaatgg gcttgaaaga gttgaccagc tacaagtatg tggttgctgg
    14941 tgatgggcag ataagggagt aagagatgaa gattggattt atcggtttgg ggaatatggg
    15001 tgctagcttg gcaaaatctg tcttgcagac taggacgtca gatgagattc tccttgccaa
    15061 tcgtagtcaa gctaaggtag atgctttcat tgcagacttt ggtggtcagg cttccagcaa
    15121 tgaagaaatg tttgcagaag cagatgtgat ttttctagga gttaagcctg ctcagttttc
    15181 tgaactgctt tctcaatatc agaccatcct tgaaaaaaga gaaagtcttc ttttgatttc
    15241 gatggcagct ggattgacct tagaaaaact agcaagtctt atcccaagcc aacaccgaat
    15301 tattcgtatg atgcctaata cccctgcttc tatcgggcaa ggagtgatta gttatgcctt
    15361 gtctcctaat tgcagggctg aggacagtga gatcttttgt cagcttttag ccaaggctgg
    15421 tctcttggtt gaattaggag aaagcttaat caatgcagcg acaggtcttg caggttgtgg
    15481 accagccttt gtctatctct ttattgaggc cttggcagat gcaggtgttc agacgggatt
    15541 accacgagaa acagccttga aaatggcagc ccaaactgtg gtaggagctg ggcaattggt
    15601 cctagaaagc cagcaacatc ctggagtttt gaaagatcaa gtttgtagtc caggcggttc
    15661 gactattgct ggcgtagcaa gcttagaagc gcatgctttt cgaggcatag tcatggaggc
    15721 agttcatcaa gcctataaac gaacacaaga actaggtaaa taagaggtag ctttgactgc
    15781 ctcttttatg gtggctgaaa tgagaagaca caaaaagatt gtcacaaact cctatttttt
    15841 tgatagaata gaagtagtaa aaaagaaatg agttagacat gtcaaaagga tttttagtct
    15901 ctcttgaggg accagaggga gcaggcaaga ccagtgtttt agaggctctg ctaccaattt
    15961 tagaggaaaa aggagtagag gtgctgacga cccgtgaacc tggcggagtc ttgattgggg
    16021 agaagattcg ggaagtgatt ttggatccaa gtcatactca gatggatgct aaaacagagc
    16081 tacttctcta tattgccagt cgcagacagc atttggtgga aaaagttctt ccagcccttg
    16141 aagctggcaa gttggtcatc atggatcgtt ttatcgatag ttctgttgcc tatcagggat
    16201 ttggtcgtgg cttagatatt gaagccattg actggctcaa tcagtttgcg acagatggcc
    16261 tcaaacccga tttgacactc tattttgaca tcgaggtgga agaagggctg gctcgtattg
    16321 ctgctaatag tgaccgcgag gttaatcgtt tggatttgga agggttggac ttgcataaaa
    16381 aagttcgtca aggctacctt tctcttctgg ataaagaggg aaatcgcatt gtcaagattg
    16441 atgctagtct ccctttggag caagttgtgg aaactaccaa tgctgtcttg tttgacggaa
    16501 tgggcttggc caaatgaaac aagatcaact aaaggcttgg caaccagctc agtttgaccg
    16561 ttttgtccgt atcttagaac aagaccagct caatcacgcc tatctctttt caggtttctt
    16621 tggaagcttg gaaatggcgc aatttttagc taagagcctt ttttgtacgg ataaagttgg
    16681 cgtcttacca tgtgagaaat gccgaagttg caagctgatt gaacaggaag agtttccaga
    16741 tgtcaccttg attaagccag tcaatcaggt catcaagaca gaacgtattc gggaattggt
    16801 gggacagttt tctcaagcag ggattgaaag ccagcaacag gtctttatca tcgagcaagc
    16861 ggataaaatg catcccaacg cagccaattc tctgctcaag gtcatcgaag aaccccagag
    16921 tgaagtttat attttcttct tgactagcga tgaggaaaag atgttaccga caatccgaag
    16981 tcggactcag atcttccact ttaaaaagca agaagaaaaa cttatcttac tcttagaaca
    17041 aatgggactt gttaagaaaa aagcgactct tttagctaag tttagtcaat cgcgagctga
    17101 agcagaaaag ttggcacatc aggcaagttt ttggaccttg gtcgatgaaa gtgaacgcct
    17161 gctgacttgg ttagtagcta agaaaaaaga aagttatcta caggttgcca aattagccaa
    17221 cttggcagat gataaggaaa aacaggatca ggttttacgg attcttgaag ttctctgtgg
    17281 gcaggacctc ttgcaggtaa gagtaagagt gattctacaa gatttactag aagctagaaa
    17341 aatgtggcaa gctaatgtca gctttcaaaa tgccatggaa tatctggtct tgaaagaaat
    17401 ataaactcaa aaatgaatga taaagaaagg aaagggctgt tttatggaca aaaaagaatt
    17461 atttgacgcg ctggatgatt tttcccaaca gttattggta accttggccg atgtggaagc
    17521 catcaagaaa aatctcaaga gcctggtaga ggaaaataca gctcttcgtt tggaaaattc
    17581 taagttgcga gaacgcttgg gtgaggtgga agcagatgct cctgtcaagg ccaagcatgt
    17641 tcgtgaaagt gtccgtcgca tttaccgtga tggatttcac gtatgtaatg atttttatgg
    17701 acaacgtcga gagcaggacg aggaatgtat gttttgtgac gagttgctat acagggagta
    17761 ggcatgcaga ttcaaaaaag ttttaagggg cagtctccct atggcaagct gtatctagtg
    17821 gcaacgccga ttggcaatct agatgatatg acctttcgag ctatccagac cttgaaagaa
    17881 gtagattgga ttgctgctga ggatacgcgc aatacaggtc ttttgctcaa gcattttgac
    17941 atttccacca agcagatcag ttttcatgag cacaatgcca aggaaaaaat tcctgatttg
    18001 attggtttct tgaaagcagg gcaaagtatt gctcaggtct ctgatgccgg tttgcctagc
    18061 atttcagacc ctggtcatga tttagttaag gcagctattg aggaagaaat tgcagttgtg
    18121 acagttccag gtgcctctgc aggaatttct gccttgattg ccagtggttt agcgccacag
    18181 ccacatatct tttacggttt tttaccgaga aaatcaggtc agcagaagca attttttggc
    18241 ttgaaaaaag attatcctga aacacagatt ttttatgaat cacctcatcg tgtagcagac
    18301 acgttggaaa atatgttaga agtctacggt gaccgctccg ttgtcttggt cagggaattg
    18361 accaaaatct atgaagaata ccaacgaggt actatctctg agttattaga aagcattgct
    18421 gaaacgccac tcaagggcga atgtcttctc attgttgagg gtgccagtca gggtgtggag
    18481 gaaaaggacg aggaagactt gttcgtagaa attcaaaccc gcatccagca aggtatgaag
    18541 aaaaatcaag ctattaagga agtagctaag atttaccagt ggaataagag tcaactctac
    18601 gctgcctacc acgactggga agaaaaacaa taaagggaga taggatgtaa taattctgtc
    18661 tgtttctgtt taacttaatt agtgatgata atataaagat gtatcacttg gtatagaagc
    18721 tttggtatta agttttttat taagcccata cggaataccg atggttggag cagcagttat
    18781 agcgttctta gaaggtataa atagaaaaat aaggtcattt taaatcaaag gattgataaa
    18841 tcagaaagaa ggtgattttt tgcgaacata cgaaaataaa gaagaactaa aagctgagat
    18901 agagaaaaca tttgagaaat atattttaga atttgataat attccagaaa atttaaaaga
    18961 taagagagct gatgaagttg acagaactcc agcagaaaac cttgcttatc aggttggttg
    19021 gaccaacttg gttcttaaat gggaagaaga tgaaagaaag gggcttcaag taaaaacacc
    19081 atcggataaa tttaaatgga atcaacttgg tgaattatat cagtggttca cagataccta
    19141 cgctcattta tctctgcaag agttgaaagc aaaattaaat gaaaatatta attctatctc
    19201 tgcaatgatt gattcgttga gtgaggaaga attatttgaa ccgcatatga gaaagtgggc
    19261 tgatgaagcg actaaaacag cgacttggga agtgtataag tttattcatg taaatacggt
    19321 tgcacctttt ggaactttca gaactaaaat cagaaaatgg aagaagatag tattataaat
    19381 tatattttta actttaaaaa atttcataaa aatggttacc aaaggcgata gaagaaaaac
    19441 tatcgtcttt ttctttgcaa atttttaaga agggaggtga tcttgcatgg actttgaata
    19501 tttttataac agagaagcgg aaagatttaa cttcttaaaa gtaccggaga tattagttga
    19561 tagagaagaa tttcggggct tatcagcaga agcaattatc ctttattcca tacttcttaa
    19621 acggacagga atgtcattta agaataactg gatagacaag gaaggcagag tatttatcta
    19681 ttttactgtc gaagaaatta tgaaaagaag aaatatctca aagccaactg ccataaaaac
    19741 attagatgag cttgatgtaa aaaaaaggaa taggactgat cgaaagagta aggcttggac
    19801 ttggtaagcc gaacatcatt tatgttaaag actttatgag tatatttcag gtaaaagaaa
    19861 atgacttaca aaagtcaaaa aacttaactt cagaagtaaa agattttaac ctcagaagta
    19921 aagaaaatga acttcaagag gttaagaacc ttgactctaa ctatatagag aataataaga
    19981 gtaagtatag taagagagaa tatagttttg gtgaaaacgg acttggaaca tttcaaaatg
    20041 tgtttttagc tgttgaagat atatcggatt tacaaatcat aatgaactca cagcttgaga
    20101 attaca
//
LOCUS       DBERYS010000037        18632 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.037, whole genome shotgun sequence.
ACCESSION   DBERYS010000037 DBERYS010000000
VERSION     DBERYS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.037"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..863)
                     /gene="lytB"
                     /locus_tag="ACL8LA_001505"
     CDS             complement(<1..863)
                     /gene="lytB"
                     /locus_tag="ACL8LA_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HGS1813248.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGG"
     gene            complement(905..1843)
                     /locus_tag="ACL8LA_001506"
     CDS             complement(905..1843)
                     /locus_tag="ACL8LA_001506"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGS1813249.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1854..2654)
                     /locus_tag="ACL8LA_001507"
     CDS             complement(1854..2654)
                     /locus_tag="ACL8LA_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGS1813250.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2904..3284)
                     /locus_tag="ACL8LA_001508"
     CDS             complement(2904..3284)
                     /locus_tag="ACL8LA_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1813251.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3342..3701)
                     /gene="rplT"
                     /locus_tag="ACL8LA_001509"
     CDS             complement(3342..3701)
                     /gene="rplT"
                     /locus_tag="ACL8LA_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGS1813252.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3753..3953)
                     /gene="rpmI"
                     /locus_tag="ACL8LA_001510"
     CDS             complement(3753..3953)
                     /gene="rpmI"
                     /locus_tag="ACL8LA_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGS1813253.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3986..4516)
                     /gene="infC"
                     /locus_tag="ACL8LA_001511"
     CDS             complement(3986..4516)
                     /gene="infC"
                     /locus_tag="ACL8LA_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGS1813254.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4532..4663)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4823..6004)
                     /locus_tag="ACL8LA_001512"
     CDS             complement(4823..6004)
                     /locus_tag="ACL8LA_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813255.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     gene            complement(6008..6595)
                     /locus_tag="ACL8LA_001513"
     CDS             complement(6008..6595)
                     /locus_tag="ACL8LA_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813256.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            complement(6628..6852)
                     /locus_tag="ACL8LA_001514"
     CDS             complement(6628..6852)
                     /locus_tag="ACL8LA_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813257.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYDSYSEYPVGSGWNYGRYEIINYYSGGY"
     gene            complement(6994..9234)
                     /locus_tag="ACL8LA_001515"
     CDS             complement(6994..9234)
                     /locus_tag="ACL8LA_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGS1813258.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(9218..9868)
                     /locus_tag="ACL8LA_001516"
     CDS             complement(9218..9868)
                     /locus_tag="ACL8LA_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HGS1813259.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            complement(9936..10505)
                     /locus_tag="ACL8LA_001517"
     CDS             complement(9936..10505)
                     /locus_tag="ACL8LA_001517"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813260.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10682..11793
                     /gene="ald"
                     /locus_tag="ACL8LA_001518"
                     /pseudo
     CDS             10682..11793
                     /gene="ald"
                     /locus_tag="ACL8LA_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(12094..12420)
                     /locus_tag="ACL8LA_001519"
                     /pseudo
     CDS             complement(12094..12420)
                     /locus_tag="ACL8LA_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12423..12923)
                     /locus_tag="ACL8LA_001520"
     CDS             complement(12423..12923)
                     /locus_tag="ACL8LA_001520"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813261.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            complement(13116..14084)
                     /locus_tag="ACL8LA_001521"
     CDS             complement(13116..14084)
                     /locus_tag="ACL8LA_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGS1813262.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(14170..14385)
                     /locus_tag="ACL8LA_001522"
     CDS             complement(14170..14385)
                     /locus_tag="ACL8LA_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGS1813263.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(14394..15248)
                     /gene="cvfB"
                     /locus_tag="ACL8LA_001523"
     CDS             complement(14394..15248)
                     /gene="cvfB"
                     /locus_tag="ACL8LA_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGS1813264.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(15308..15865)
                     /gene="frr"
                     /locus_tag="ACL8LA_001524"
     CDS             complement(15308..15865)
                     /gene="frr"
                     /locus_tag="ACL8LA_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGS1813265.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(15874..16611)
                     /gene="pyrH"
                     /locus_tag="ACL8LA_001525"
     CDS             complement(15874..16611)
                     /gene="pyrH"
                     /locus_tag="ACL8LA_001525"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGS1813266.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(16695..18029)
                     /gene="trmFO"
                     /locus_tag="ACL8LA_001526"
     CDS             complement(16695..18029)
                     /gene="trmFO"
                     /locus_tag="ACL8LA_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGS1813267.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(18275..18547)
                     /locus_tag="ACL8LA_001527"
     CDS             complement(18275..18547)
                     /locus_tag="ACL8LA_001527"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS1813268.1"
                     /translation="MFTCFIIHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSG
                     KIKYHEDSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            18524..>18632
                     /locus_tag="ACL8LA_001528"
     CDS             18524..>18632
                     /locus_tag="ACL8LA_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1813269.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAY"
BASE COUNT         5372 a         4218 c         3442 g         5600 t
ORIGIN      
        1 ccaccggatt tgaagtagta ccaagcttga ctatgagaat cgtagaccca ttctttttca
       61 gctattttcc catcaaactt gaggtaaaac caagattcct tatcccaaat ccattcattg
      121 gctgccatgt agccaccgga ttttagataa taatagtgac cattctcgaa aatccattct
      181 ttatcagcat agtttccatt ttctttgatg taaaaccaag attggtattg tttgtcaaaa
      241 agccaacctt gctgtacttt ggcaccacta gcattcacat aagcttgatt aatccactga
      301 cttgtcagta gataaccacc ggatttgaaa taatagtcct tccctttaat ttggagccat
      361 tctttctctg cgtgctgtcc atctgctttg atataaaacc aagcatcgta ttgagaatca
      421 tagacccagt cttctattac tttggcacct gttgcaccaa cataggaagt tcctacccaa
      481 gcatttcttt tcatctttcc atcttggtca agataataaa aggctccctt gtcttctacc
      541 cattctgatt tggccatata gccaccagat ttgaggtaaa aatagtcgtc accttgcttt
      601 agccattcat tttcagcata gttagcatct gcttttatat agaaccaaga ttgataatga
      661 gtatcaaaaa cccactcatt cgctgcttga ctgccatctt ctttcagata ctgttttcct
      721 tgccaagtac catcactagc cattgcaccc tctggactag ctaagaaaaa tagcagagct
      781 aaaaaaataa atcttacttt cttcattaac cttcttcctc tgttcttatt tattttattg
      841 taccaaaatt ctcctaaatt catattacaa actaataaaa aatcaagaac agattgattg
      901 cagtttacct cagagactct tttacttcct gacggagttc ttccagactg ctaataccgt
      961 atttatccat gacttttggt aaattttcga tgatgtcagg gcaggcataa ggattggtaa
     1021 agttagctgt tccaactccg atagcagatg ccccagccag atacatttct agggcagctt
     1081 cagccgaatc cactcccccc attccaatga taggcaggtc tgttgtttgg gctacttggc
     1141 ggatgagttt gagggctact ggaaagactg ctggacctga cattccacct gttccattgg
     1201 ccaagattgg ttttctggtt ttgaggtcaa agcgcattcc aaccagagta ttgatcatgg
     1261 tcaagccact tgctcccgca tcttctgcag cttttgcgac agtaacgata tcggtcacac
     1321 tcggggttaa tttgacataa actggcactt ctgaggcttc cacagctgct ttcaccacat
     1381 cataagccaa atctggatct tgaccaatca aaagtccatg attacagtgg tcaacattgg
     1441 gacaagaaat attgagctcg atagctttta cattagttac cttggaaatc ccatgagaaa
     1501 cagctgcata ctcttgtttt gaaaaaccag ctacattggc aataatagga agatttggat
     1561 attctctttc cagccaaggt agcttttcag ccaaaacaac ctctaaacca ggattttgca
     1621 agccaattgc attgagcata ccagcaggcg tctctgccac tcttggagtt ggatttccaa
     1681 aacgtggttc aagggttgtc gccttgatca taatagaacc taaaaggtct aaatcatagt
     1741 acttggcata ctcttgtcca aagccaaaac agcctgatgc tggaataatc ggatttttca
     1801 aatccaaacc aggtagagaa acttgtaatc gatttgtagt cataattttc tccttataat
     1861 acaactgttc ctgtgcggaa aacaggacca tcttcacaga cgcgttggct gaccgtctcg
     1921 ctttctggta cttttagaac acaggcatag caagctccca tcccacaagc catacgagat
     1981 tccagagata aataggctct tgggtgatca tcaaaggttt gattgatata cttcatcatt
     2041 cctggagccc cacacgagta aacagcatca aactgactgt ctaaatcatt gataacaacg
     2101 gaaacatttc ccttgatgcc ataagaacca tcatctgtcg ttacaaagac ctgaccatac
     2161 tgagccaatt ccgttttcaa aataacagca tccttattag caaaaccgag gactgtcact
     2221 actttcactc cacgttcatg caattccttg gccacctcaa gcaagggtgg aacaccaatc
     2281 ccaccaccaa caaggagaac ctgattctgc tcatcaaggt cagacaagtc aaaaccattt
     2341 ccctgaggcc ccatcacatc aagagtgtct ccctgactta aggttgaaaa aattgcagtc
     2401 ccagctccct caatccgata aatgaggtga cactgcttgt ttgccttgtc aatagacgaa
     2461 attgaaatag gacgacgtaa gagatgggca tcgtctggta cacgcagatg aagaaattgg
     2521 cctgctcgca tggcttcaac catttctcct tctaggacta attcaaagat tgctggcgcg
     2581 atttcctctt gtgcaaccac cttcatggtt tccaaccgaa tgacacccaa acgcttctta
     2641 catgtgagat tcataacttt tcctccttaa attttaaggg gaccagataa agaaaagacc
     2701 ttgctagtgc aaggcctcag ttaaaatcgt acaacacaag agagcacact caaaaggtct
     2761 cctctagaaa cttgcactat tcttttatct gacaccttgt gagtctctct ggactcccct
     2821 taaaggttga atttttatta gtatactctt tcagctaaaa aaagtcaagt agaaaacgaa
     2881 tattctactt aacttgcacg aaattatttt tcacgaatga cttcgacctt atatccatca
     2941 gggtctttaa caaagtaata gtttggtgta gttcctggta gaccatttgg ctcagtcact
     3001 tcatagcctt ttgcactgtg ttcttgatga agtgcctcaa gatcaggtgt actgagggcg
     3061 atatgggcaa acccatcacc aaccacatac ggaccgtgat cgtagttata agtcaactcc
     3121 aactcataat catcaccctc aagacctaga tagacaatcg tgaaggcatg gtctggaaaa
     3181 tctctgcgac gcaattcttt aaaaccaaaa gcatcttgat aaaatgcgat tgatttttca
     3241 agattttcta ctcgtaagca agtgtgtagc atttttgaag ccatatcttt ctcctttatt
     3301 tttaaaaaga ctggacaatc ctgttccagt ctcatcagtt gttatttacc aagttttgct
     3361 ttagctgcat ctgcaagagc tgtgaaagct actgcatcgt taacagccaa gtcagcaagc
     3421 attttacggt taacttcgat ctcagccaat ttcaaaccat gcatcaattg tgagtatgaa
     3481 agtccgttca tacgagccgc cgcattgata cgagtaatcc acaatttgcg gaagtcacgt
     3541 tttttctgac gacggtcacg gtatgcatag tagtaagagt tcattacttg ttcttttgca
     3601 gtacggaaca agatgtgttt agctccatag taaccttttg ctaatttaag aatacgttta
     3661 cgacgtttgc gtgatacaac gccaccttta acacgtgcca tgttttattt cctccaaata
     3721 ttttctagaa ttgtttactt acagtcaagc tattatttca agcgagtaag cattgctttg
     3781 atacgtttgt aatctcctga atgcaccata gatgctttac gaagatgacg acgttgtttc
     3841 ttagtttttc cgtggaaacg gtgagaagtg taagcacgga aacgtttaag tccaccagaa
     3901 cctgtacgtt tgaaacgttt agctgatgcg cggtgtgttt tttgttttgg catgattttt
     3961 tctcctttat ttaactttct gacaattatt ttttgtcagt cgctggcgcc aattgcatga
     4021 acatttgacg tccatccatt ttagcacgtt gttcgatgat ggcaatatct tgagttgctt
     4081 cagcaaactc ggctaaaact tttgcaccaa tctctttatg ggtaatcata cgacccttaa
     4141 agcgaataga taccttaact ttatttcctt tttcaaggaa cttacgtgca ttacgaagtt
     4201 ttgtatcaaa gtcacccttg tcaattgttg gacttagacg aacttctttc acagtaacaa
     4261 cgctttgttt tttacgttgt tctttttgct tcttctggta ctcaaatttg aacttaccgt
     4321 agtccataat ttttgcaaca ggcggtttgg cttggggttg aatcaatact aggtcaacat
     4381 tagcgttatc agccaaagct tgcgcttcac tgagtggctt gatgcctagc tgttctccct
     4441 caagaccaat caagcgaact tcacgtacac gaatctcatc attgatgaat aagtcttgct
     4501 ttgctatggt tttcacctct tttgttttat tagagaaaaa caatagcgga ctcgtaaata
     4561 tacaagcccg cacgttatga tagcgtttct tcgaaacttt tcatccgtag ggccaggcaa
     4621 cttaatgtca caaggcgaga agctctcact tctgcttttc tcaactttta tatgatacca
     4681 agttttctag ctcttgtcaa gataaaaagt tattttttga aaagtttgtg tttgtatttt
     4741 ggtttggttt tcgaatcaaa aagagagaac caagttgatt ctctccctat gttagttttt
     4801 ctttagcttc cctataggaa aactaaactt taaaaacttc ctttttacgt gaacttcccc
     4861 atctttcgat aaatagaatc cccactaaca aaaggataat caggcaagga agtaagacca
     4921 tccccatgct ccaatttaga tcgacattat caatctgctt aggtaatctt ccagataaaa
     4981 tctccactga acgcaagtaa gtaaagggaa tcagatgtgc aatcttttga agaggctgaa
     5041 tggtttggat gccaaacaat aagccaacaa tcccaatgag tgaaagaaag aggacaggca
     5101 ttttttgctt gaaaaagtaa gcaatcaaat acacaacttc cacaatgacg ataaaggcta
     5161 agaaagctaa gagcaagcca ggaaataata catcctgcat ctttccaata gttacctctt
     5221 gattcactaa gctataaatc gggtagggat aatctaactg tccaaaacca cttatcagac
     5281 ttcccactag aaaagaaaag ccacagattc cgataaacag cacagttaca tatcccactc
     5341 caactccaag agaggatatt gcaaatgtca cttttgaaac aggatataag tgagctgtgt
     5401 ccagatgatt ttgatatctt tctgcaaata gttgtgttag cataaaaata atcgcaacca
     5461 caaacaagct tgggatgata acctctaaaa tccagacaat ctgatcaatc ccgtgggtcg
     5521 gaaactctaa agtatgtgct tttatgttca agggatacag ggcttggtaa atcttccgtt
     5581 cgcggtcaac ccccattttt aagccagagc tagcagtcgg gtcatttgat acaaattcat
     5641 aattcttctc ttcatcttgc cactgcaaat agtaggcttc tttccagcgc ccttctttta
     5701 gtaaagttag aatttctgtc tttcgcgtca aaagattttt ttgcacgtct aaattatttt
     5761 tagcaaactg gtattcctcc gagctggtat cagacatttg ggagagtttc tcttcatttt
     5821 cattgatagc cctctcgttg gctgcaatgc gactttccaa cctgctctcc aagctgtgtg
     5881 agtttgcagt ctgactattt aaataaaagg taacaccgag tacagataca aataaagcta
     5941 agacaatcca gtttaagcgg cttttgaaaa cttttttcaa taaaaataga ctaacatctt
     6001 tcataaacta aacctcttct atctgcccct gatgaatggt tactactcta tcgcagacat
     6061 caaccaactc ttccttatag tgggaactta aaagaaccag ctgttcttgt ctatcgattt
     6121 gtgctagcct atcaaaaaac ttctgtcgat aatactcatc taagccattt gtaatctcat
     6181 ccatgagcca gcatttggcc tgactgagga aatacatggc aatcaccaag cgttgcttca
     6241 tgcctaagga atacttgcga atgggaagac tgatatagtc agacatttcc caataggcga
     6301 tttcatccct caagttcaga cctgacttcc agatgttttt gataagacga aggtagtcca
     6361 tcccacttaa gtttccatcc agccattcaa tactctcata ataaaataaa gaaggagggg
     6421 ctgcgatatt tccactactt atgggaatta aattgctaat ggcacggaac agggtcgtct
     6481 ttccagagcc attgatagca agaagaccat aaaccctacc ctttttaaag gtgaaatcca
     6541 catcttgcaa gatgacttgt cgcgttttta aggtcacatg agtaagctgc aacatatcta
     6601 gccctccttt ttctcactct ttaagaatta atagcctccg ctgtaatagt ttatgatctc
     6661 ataacgaccg tagttccagc cagaaccaac tggatactca gaatagctgt cataacgaga
     6721 ccatgacgga atccaagcat actgatggtc gtgatagttg gtactatatt cccaaaccgt
     6781 attccaatca tacttgtaac ttttagtgac tgtcacagca gatatactgg actgaaaaag
     6841 accaataatc ataaactaac taattaaacg atttttgcta atttttaatg attttatatc
     6901 ctcaatattg aatacgtttt cattatacca tttttatcat tattatgcaa caaattttta
     6961 gtttatttca ctaatctaca acatttatcc ttcctatcga acactttcga ttttccaact
     7021 atccaacccc ttaaaacgta tagctccttg ctggtcagtt cgataaactt tgctattgat
     7081 accttccagt cgtgtcaatg tttcctgatg ggggagtttc attcgattgc tctttccaac
     7141 tgagataaga gtaagctctg gtttgagttt ttctagaaag gctggacttg atgatttttt
     7201 attgccatgt tggctagctt tcaaaacatt tactttcaag tctggatagt gcttcagcaa
     7261 gtccttctct cctttctcct ccaaatttcc cgtgaagaga aattgcttat ccaagaattt
     7321 cccatacaga actagggtat catcgtgtcc tccatctccc attttccttg gagatagaac
     7381 ttctaactga cttccaaaaa tgggcaagtt ctcccctact gtcatactac gcacctttgt
     7441 ttgagtcgcc tgtagttctg ccacaaattc cttctgtttc agactgtctt ttgatactag
     7501 aatctcccct acatggaaag ccttggtcac ctctgacaaa tctccaacat gctccttgtc
     7561 cgtgttagtc aaaattagct ggtcaatctt agctactcct cgacttttga gatagggaat
     7621 caaggttcgc tgggcattgc tggtcgtcat tttttcttgc catttttcga ttttcttata
     7681 agattctgcc ttaccaccta catctatgag aatggttttc ccagttacat cccgtaggaa
     7741 aatactttct ccttgcccca catccagcat ggtgatttca ttttccagtg gatgcttggt
     7801 caggagaaag agccctgtaa tcaataagca caataccgtt agctttttaa tgttttttct
     7861 caaatcatag accaaagcca aggaaattaa caataggatt aaaaaccatg cattgggttg
     7921 tccaaagacc agaggtctac ttgtcacctg tgacaccaag cgaataatgc cctccaacca
     7981 ttcaaagata aagttcagct gaatgactgg atagagaaag gaaaggacaa ataaaataga
     8041 caagagcggt aagaaggtca agtcaaatag aaaggaaaag acaaaggtca aaaggataga
     8101 caaaggttga aattccgcaa aatagaagga tagaatgggc aatatgccca aggagatgac
     8161 tagactttca ctagtaacag ccttgagccc ctccccttct ttgctggtca tggtcaggat
     8221 aaaagcataa gcgcaggaca agactcctcc tgctgtcaag aaaaagtttg gcatgacaat
     8281 aaagaggaca agcaccgtca aggcaaaatt atccaagccc ttaaccccat gttgagccag
     8341 tagcttttgc aagagactgc gaataaccga tgctgaaaat ccagttagtc ccgcatagat
     8401 aagggaaaag ggataagtca gccatttcaa cttttcttgg gtcaagccca atcgcaagag
     8461 aagtttctta aatccattca tgaaaaaacc tacctgcata ccagataggg caaagaggtg
     8521 gataattcct agactggaat aaagctcatt catctcctca aagtcggtgt ccagatgtcc
     8581 cagcaagagt cctgtcatgt aattgcgcat agggtctgga aagtgcgtct taatccaaac
     8641 cacagccttt cgacgtaaac tggacaagtt ttctcctata tcccaactgc caatcttttg
     8701 aagtgactgg attgttttga tattgagagt ctggtaaatt ccctgagtct tcagataggc
     8761 ttggtaatta aagccaccaa aatttctctg cccttctggc tccgaaagct tcccttctag
     8821 tcctatctca tgcaggtcgg ttaaagcttg aaaggcttct ttctcctcct cggactggag
     8881 tttataatag acttggaaag cacgaccgtt agacttgcca cgaaaggata gactatcacc
     8941 attaacctta atagtatcag gcaaaatccg taccctttca acagaatccg ccagattttg
     9001 actcgcttga ctctgttgcc aattttgaaa aacaaaccaa aatccaaaga ttccgcaaat
     9061 tattagaact ttaccagcag atttccacgg aaattggata aagagacaga ctagcagaaa
     9121 aacaaagccc aacaaagcaa gataagatgc tgagaaaata gcgtaataaa gccaaagtaa
     9181 tagaaaactc aggtaaatta ggggaataga gaaattctta atccactgta acatagtctt
     9241 taagcttttc tattgttttg ccaccaatgc cagagacctt cttgagctcg tctactgact
     9301 tgaacttgcc atttgcctca cgatggtcga taatgtcctg agctcgtttt cctcccagtc
     9361 ccttgacctg cttgagttct tccagactgg ccttgttgag attgaccttc ttttccttgc
     9421 ttattgaaga agctgtcccc gaaccagtct gttgactaac tgcttcttct cccttagtag
     9481 gaacgtaaac cagagcctca tcactaactt tctgagctag attgagcgac ttgctgtctg
     9541 cttgctctgt caagccacca gccttctgaa cagcatcatt gactcgacta cctacaggca
     9601 agtcataaat ccctggcgat ttgacagcac ctttgacatc tactgtgatt agatcttgtt
     9661 caaggggttc ttccttctct tccttgttca cttccttttc ggataccaaa tccttggaaa
     9721 cagctgcaac ttcagcctgc aaattcgttt cctttacagg tgtttgtgga gctggcttta
     9781 gcaggaaaaa tccgcctaca agcaagccca gaccagtaca gatgacgatg attttatact
     9841 ctttgatttt ctcgataatt gcttccatat tttctcctct cttagattat tcgtaagagg
     9901 aagaaaaaac agtcgaaaaa tttcttttca actgcttatt tatttgcaaa atagtcttcc
     9961 ttggtcaaga cataatgaac tcttgtcacg attcggcctt tttcatgctg gtccatacaa
    10021 gcatatggtt ctgcatggga aaaacgcatg cctgatttct ccatgacctt tcctgacgca
    10081 gaattgtcct tatcgtataa agcaactaac ttattcattc ctatcttctc aaaagctagc
    10141 tcaatcacag cacgattggt ttctggcgtt aatccttgat tccaatactt tttattgata
    10201 atgtagccaa tagctgcctt cttaagaaca ggatcaatct tgtgtaagtc aatagttccg
    10261 ataaacttgc cgttatcttt taattcaatc ccccaacgtc ccaagggatt agccaagtag
    10321 aactgagcaa tgttattctt ggtttcttcc aagctttgat tggttggaaa agtgtaacgt
    10381 gtattaccct tgtccgaggc atagtcaaac attgcttccg catcatccaa agttacaggt
    10441 ctaagcaata aacgctccgt ttctatagac ggatactggg caaatttcac aaatattgat
    10501 tccatacttt tccctccatt agagcttgat tttcactagt ctagcataaa aaaatttgga
    10561 tgacaagttt ttcttgattt ttgaagcggt ttcatataca atgaaaccgt cacatttttg
    10621 atttatgaac tggaatgtga caaccaaaac aagaccgtca attgaaaggt aggttatctc
    10681 aatgttaatc ggaatcccaa aagaaattaa aaataacgaa aaccgtgtcg ccctcacacc
    10741 tgcaggtgtt catagcttag ttagtcgtgg tcatcgtgtc cttatcgaaa caaatgctgg
    10801 tctcggttct ggctttactg atgctgacta tcaaaagcaa ggagctgaga ttgtcgctac
    10861 tgctggtgaa gcttgggcag cagagttggt tgtgaaagta aaagaacctt taagttctga
    10921 atacggttac ttgcgcgacg atcttcttct cttcacctac ttgcacatgg ccgctgctcc
    10981 agaattagca gatgctatgt tagcagcaaa aacaacagga attgcctatg aaactgttcg
    11041 tgacaatcaa ggacaactac cgctcctcgt tcctatgagt gaggttgcag gtcgtatggc
    11101 tgtttaaatc ggagctcact tccttactaa gcaagctggt ggctctggtg ttctacctag
    11161 tggtgtacca ggtgttccaa aaggaaaagt aactatcatc ggtgatggtg tcgtcggtac
    11221 acatgctgcc cgcatcgccc ttggtcttgg tgctcaagtg actattttag atattagtgc
    11281 caagcgtctc tcagttctag aagaagtctt tggaagtcaa attcaaactc ttatatctaa
    11341 ttcattcaac attgaagcaa gtatgagaga tgctgatgtg gtgattggag ccattctcat
    11401 ccctggtgca aaagcaccgg aattggtgac agatgagatg gtcaaacaaa tgcgtccagg
    11461 ctctgtatcg ttgacgttgc tgttgaccaa ggtggcgtta tcgaaacagc tgaccgtgtg
    11521 acaacgcacg atgaacccgt ctatgaaaaa cacggtgttc tccactatgc cgttgccaat
    11581 atccctggtg cggttgcccg cacttcaacc atcgccctaa ccaatgtcac tcttccttat
    11641 atcgaagctt tggctggcaa aggattcgca caagcaatct ctgaagatga aggcttgcgt
    11701 caaggtgtga ccacttatca aggttacttg actagcctac cagttgctca aggacttaat
    11761 cgtgactaca ctgatatcaa tgatttagta taaattttga aaagaaaagg gtgacttcgc
    11821 aagtcctacc cactgtgctt tgtgtcatca gctattacat cgttggttta ttgtactgta
    11881 tactttgcaa ctggagactt tcgttccaga ctcaattgtg cataagaaac tctaaattga
    11941 ataaagtcaa cagagtttcg cccaaccact tcgtcttgta atgacaatta tataaaccaa
    12001 aggaagctga gctctttagt cccagcttct ttttatatat aaaattttac ccgtgcaaag
    12061 acagggcctt agcagacttc ttttttactt cgttcaccct tgctttttct ttttaggttt
    12121 gggcgttggc agttggttat acatagctaa aatcaggtct tatagaaaca tcttattatc
    12181 aagttcttcc actcaaatca tttctttggc acctttgtat ggaaactcaa aagaagattg
    12241 gtcaatctta tctaagactg cttgcacggg tttaactaaa aagcgatcgt cataaatgcc
    12301 gccaataatc ttgccgcgga agtaaagaat atactccccc atcatggaac ggtaagtcac
    12361 atcatctaat cctgataatt gttccaaaac aaattccaaa tagttcttac ttgatgccat
    12421 ttctaatctt ctaggctctg ttcagcgata acaaccgtat agagttcttg cttaacctcg
    12481 catccaattg atttaaagcc ctgcttttcc caaaaatgct gagattgcgg atttccctta
    12541 acataagcca aacgtgcctt tcgaaagttc ttagcaaaat aagctagtgc ttctgtcaca
    12601 atatgactac caatcccttt cctctgatag gcttgatcaa ccataaacaa accaataaaa
    12661 acagtctcct catcaggatg tgcatagaca aaatctataa cagccacaag gtcagatcca
    12721 ttccaaaatc caacaaaaaa cttatcagcc ttagctttac cttcaggtag acaaagcatg
    12781 tcctctttta cagttgcaaa atttggctct ggtggacaat gctgaaaata cagaggatta
    12841 ctttcatata aagataaaat acttggaata tccttttcag ttagtatcct acaactgtaa
    12901 tacttagata gttggtcaat catcttttca aattcctact ttcttgtgca ctgtgattat
    12961 gacacaggaa gatgcactga tcgtcatcag ccacataaaa gttctttcca tcgtgcctaa
    13021 tcgttgtctc aaacctttgg ataaaacctt tagcctatac aactggattt tcctctctca
    13081 aaagtatatt cttttgcagg caaacttcct caaaatcagt cgtgtgcaac ttcagtagaa
    13141 tattcatagg ctcggataat ctgagcgaca actggatggc gaaccacatc cttggctgaa
    13201 aaatgaacaa agtcaatctg atggatgttc ttgagtttct cttgagcatc aatcaaaccg
    13261 gacttgacat tacgtggcag gtcaatctga ctaatatctc cattgacaat catcttagaa
    13321 tgaaaaccta aacgagtcaa gaacatcttc atctgcatga tggtcgtatt ttgcgcctca
    13381 tcgagaatga caaaggcatc atccaaggtc cgtccacgca tataggcaag gggcgcaatc
    13441 tcgataattt cacgctccat gagtcgagtt gtttggtctt tccccagaat ctgatacaag
    13501 gcatcgtaaa caggacgaag gtaaggatcc accttctcct taagatcacc cggaagaaat
    13561 ccaagactct ctcccgcttc caccgctgga cgagttagga taattcgctt gacttgccca
    13621 cgtttaaggg cagtcactgc caaggtcact gcaaggaagg tcttccctgt acctgctggc
    13681 ccaattccaa aggtcacatc atgctgtttg acactgtcca cataaagctt ttgccctagg
    13741 gttttgacac ggataggttt cccagtatta tctttgataa tttcttcttc gtaaagggcg
    13801 acaaacttgt caatttcatc atttttgacc atgctaatcg cagtgactac atctggcgta
    13861 ccaacggtca tcccacgatt taccaagacc atcaaagcct gaataacttg acgggcttcc
    13921 tcacaggcag actcttctcc caaaacctgg acaatctccg tacgagcatg aatcacaaca
    13981 tcaagctctt cttccatcaa acgaagatgg cgttcattgg aaccaaaaag atgaaacagg
    14041 tcatctggat gactcagttg aatgtctatt gaatgttcct tcaaataaag aacctctcta
    14101 atccttttat ttctttttat tatagcagag ggatggagaa attactagcc caaacccaat
    14161 gaataaatgc tagtgttcta gatattcctg ccaaatgctg tcaaaatctc ctaggttaaa
    14221 agagaaactg gcatgctcct ccaaaaagcg actgacctca tcaaaatcat ctgtatgttt
    14281 tggaaaagct gcctcttcaa aagcgaggtc tgccaaaatt gctttgggac tgttactttt
    14341 aggattgcgc tcggtcatga gccaagtgta aaatgatttt ctcataagcc tccctaaatc
    14401 aactctgtcc caaactggtc ctgcttgatt ttaccagcct tcataagacc acctaaagct
    14461 ttcttgaact gacctttaga aatgccaaag gttgccttga tgtcgtctgg agatgactta
    14521 tcatttaagg tcatgaaacc gccattactt tccaaataag tcaaaatcat ctgagcatcg
    14581 ttttccaaca tttcaaagga gcgtggtttg agggagaggt tcagagtgcg gtccacttca
    14641 cggaaaccaa taacgcgcgc atctaatact tgccccaaac gtggctctgc gtaacgctcg
    14701 ctaggatgaa taaaaccaag catattattt tctggtaggt aaacaaaagt tcctgacagc
    14761 ttgagacggt aaacaatggc tggccagttt tggttctgca tgttgttgta ggcaggacga
    14821 gcaagacgtt ggaagtcttc ttgataagcc aagaggcccc agatacggtc tttcttatcc
    14881 acttcaagac ggatgtagag ttggtcgccc ttcttaggcc agagttcctt gagctcaggg
    14941 agaatatcga gtgacacaac gatttccttg tcaggaaggc ctgtatccac aaagacaccc
    15001 aaatccttac gaacctctgt gacacgtccc caaccaaatt ggtcctgagt ggcagtcact
    15061 tctaaggttg tcaggcggag tttttgcttc atatccgtgt atgcaaaacc tttgaccgta
    15121 tcccctactg tatgttgacc ttcttcctta gcaagagcat aggtttgacc atccttttgc
    15181 acaaagtaaa aacggtcatt ttcatcgatg atcagtccaa cgataaaact tgcaagattt
    15241 gtattcatat ttccttcttt cgaataaaac tcagccagca atgccaactg agtttttctg
    15301 tttattttta gacttccaaa agttctttct ctttgttagc agtcatgtcg tcgatgtgtt
    15361 taacagcatc gtctgttact ttttgaatat ctttttcaag agtcttcaat tcgtcttcag
    15421 tgatttcttt tgctttttct tgtttcttag cttcgtccat agcatcgcga cggatattgc
    15481 ggacagccac tttagcattt tcgccgacct tcttcacttc tttagcaagg tcacgacgag
    15541 tttcttctgt aagagctggg ataaccaagc gaatcacaga accgtcatta gccggtgtga
    15601 taccaagatc agaagcgttc aaggcacgtt cgatgtcttt caatgaagac ttgtcaaatg
    15661 gtgttaccaa caaaacacgc gcttctggaa tcgtaattga agcgatttgg ttaagaggag
    15721 tttcgactcc atagtattct acatgtacac ggtcaagcaa gcttgcattg gcacgaccag
    15781 cacggatacc accaaattca cgagcaagtg attggtgaga ttgggtcatt ctctctttag
    15841 ctttttcaat aattacgtta gccatattct ttcttattcc ttttcttcga tattatttga
    15901 aactgttgtt ccgatatttt caccaaatac gacacgtttg atgttgcctg attggttcat
    15961 gttgaagaca accaagtcaa tgtcgttgtc cattgagagg gttgaggctg ttgagtccat
    16021 gatacgaaga cctttgttga taacatcacg gtgggtcaat tcttcaaact taacggctgt
    16081 cttgtccttc ttaggatcgg cattgtacac accatcgacg ccatttttag ccatgaggat
    16141 ggcatctgct tcgatttcag ctgcacgaag ggccgctgtt gtatctgtcg agaagtatgg
    16201 tgaaccaatt ccagcaccaa agataacgat acggcctttt tcaaggtgac gaagggcacg
    16261 tccacggaca taaggctctg ccacttgttg catagcaata gctgtttgta cacgcgtatc
    16321 aaccccaact tgttgcaatg aatctgccat cacaagagca ttcataacag tcccaagcat
    16381 tccagtgtaa tctgcctgaa cacggtccat acctgcttct gctgcaggtt ctccacgcca
    16441 aagatttcct ccaccaataa caagggcaat ttcgatacct aagctatgaa cttcttgaat
    16501 ctcttttgcg attgtttgaa ctgtttggat atcaatccct acgccacgtt caccggcaag
    16561 ggcttcacct gataacttga ttaaaatacg tttatacttg ggattcgcca ttttcactct
    16621 ccttctttca tcctacctat tttatcacaa tttctaagat ttttatagta tcatgagcaa
    16681 ttctttcaaa aaaattagac agtcaaaaat tcctctaagt cggcaagggc acgctctgca
    16741 attttttcat aacgagcctt cttatcacgg atacgctcgc cttccaactc cttgatgatc
    16801 ccaaaattga cattcattgg ttggaaatgt ttgctgtcgg catgggtaat gtaatgagct
    16861 aagcttccaa tcgctgtcgt ctcggggaaa ataacctcgc tttcttcctt gaagagacga
    16921 gctgcgttaa ttcccgcaac taagcccgaa gccgccgact caacatagcc ttccaccccc
    16981 gtcatctgac cagcaaagaa gagatttggt tgtttcttgg aacggtaagt ctgctcaaga
    17041 agatttggtg aatccatgta agaattgcga tgcatgacac cataacggac aaactccgca
    17101 ttttcaagac ccggaatcat ttggaagaca cgcttttgtt ctccccattt gaggtgggtc
    17161 tggaaaccaa caatattgta taggctacca gctgcattat cctgacggag ctggacaacc
    17221 gcataaggtg ttttaaattc tccatcacga ggtcctgtgt agtcgtccgg atactcaaga
    17281 ccaactggtt tcataggacc ataaagcatg gttttaatgc cacgtttggc catgacttcg
    17341 ataggcatac atccttcaaa gtacttttct ttttcaaaag aattaagcgg tgcttcttct
    17401 gcattgacca aagcttcatg gaaatccata aattcttgct tggtcatagg ggcattgagg
    17461 taggccgctt ctcccttatc ataacgtgat ttgaggtaga ccttgctcat atcgatagtg
    17521 ttgacatcga taataggcgc tgccgcatcg tagaaataaa aaccatcacc gtcattaagg
    17581 gcatgaatct tttcagccaa ggcatcactt gtcaaaggac cagtagcgat aaccgtaata
    17641 acatctgtcg gcaattctgt aatttcatca cgaaccactt caatcaaggg gtggttggca
    17701 actttttcgg tcaccatttg agagaaacca tcacggtcca ctgcaagggc accacctgca
    17761 ggaacacgtg tagcctcagc agattccaaa atcacagaac ccaagcgacg catttcttcc
    17821 ttaaggaggc caacagcatt tgtcaaagca tctccacgca aggaattgga acaaaccaac
    17881 tcagcaaaat tgtctgtttt gtgctgaggt gttgacttga caccacgcat ttcataaagt
    17941 ttaactggaa taccacgctc tgcgatttgg taggctgctt cagaacctgc caaaccagca
    18001 ccgataacat tgatataaga ttgagacacg acactaatac ctctttggga gtatgaagtt
    18061 aagattcaca ttgaaaaagc caatcagact tacgagcttt cgagtttctt ggctcaggct
    18121 gaaaaagtcc acaggacttt ttcactccca caaatctttc tatttttttc ttctactagt
    18181 ataacaaaaa aagggaagaa ggcaaacttc cctgtttagt cattttcttg atgtaaagag
    18241 ataaagagta ttccagttaa gaatcaatta tatgctactc tataaaatct tttccacata
    18301 acggatcgat agggactgtt attctatctc ttgctataac cgtattatct aaaacagcat
    18361 aacattcaac atagtgatct cctttaaact gtgaatcttc gtgatatttt attttacctg
    18421 aaaaaatagc accacgctca caatttcttc taatagcctc agcttcaata tttcttactt
    18481 tccaatagac ttcctgcgaa acaaaatatg gtatagtagt tctatgaatg atgaagcaag
    18541 taaacaacta actgatgcac gatttaagcg tcttgttggt gttcagcgca cgacttttga
    18601 agagatatta gctgtattaa aaacagctta tc
//
LOCUS       DBERYS010000038        18128 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.038, whole genome shotgun sequence.
ACCESSION   DBERYS010000038 DBERYS010000000
VERSION     DBERYS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.038"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1879)
                     /locus_tag="ACL8LA_001529"
     CDS             complement(<1..1879)
                     /locus_tag="ACL8LA_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGS1813270.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVMGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(1873..2742)
                     /locus_tag="ACL8LA_001530"
     CDS             complement(1873..2742)
                     /locus_tag="ACL8LA_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1813271.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLYRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(2826..5471)
                     /locus_tag="ACL8LA_001531"
     CDS             complement(2826..5471)
                     /locus_tag="ACL8LA_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGS1813272.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(5562..6842)
                     /locus_tag="ACL8LA_001532"
     CDS             complement(5562..6842)
                     /locus_tag="ACL8LA_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGS1813273.1"
                     /translation="MIYPKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7013..9097
                     /locus_tag="ACL8LA_001533"
     CDS             7013..9097
                     /locus_tag="ACL8LA_001533"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGS1813274.1"
                     /translation="MKPLLETIDTRFGTASKHAFSWGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKILVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9137..10816)
                     /locus_tag="ACL8LA_001534"
     CDS             complement(9137..10816)
                     /locus_tag="ACL8LA_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGS1813275.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQQVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLYFYLNLTVDGQLVDQLQVQVS"
     gene            11572..12801
                     /gene="arcA"
                     /locus_tag="ACL8LA_001535"
     CDS             11572..12801
                     /gene="arcA"
                     /locus_tag="ACL8LA_001535"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGS1813276.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            12859..13875
                     /gene="argF"
                     /locus_tag="ACL8LA_001536"
     CDS             12859..13875
                     /gene="argF"
                     /locus_tag="ACL8LA_001536"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGS1813277.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14040..14987
                     /gene="arcC"
                     /locus_tag="ACL8LA_001537"
     CDS             14040..14987
                     /gene="arcC"
                     /locus_tag="ACL8LA_001537"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGS1813278.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15198..16709
                     /locus_tag="ACL8LA_001538"
     CDS             15198..16709
                     /locus_tag="ACL8LA_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGS1813279.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            16731..18062
                     /locus_tag="ACL8LA_001539"
     CDS             16731..18062
                     /locus_tag="ACL8LA_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGS1813280.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5062 a         4136 c         3763 g         5167 t
ORIGIN      
        1 aagtcgtata aatcgtcgaa gctgtcgcaa tggtatgcca ctggttagct gtagttgctg
       61 cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat ttcaacttcc agctcgtcga
      121 tgcggtcaag ctggccagcc agataaacct cgatacggct ttctgctcgt ttgatgcgtt
      181 gcaagagtcc gcccatacgg atgtcaactg tatccaaacc aaagaccttg ttttctttca
      241 accattggtg actaaagagg gcatggaagt cttcaatttg gcttctaagt tctggtaatt
      301 cttgtctggc gatttgttgt aaactttctt tatcatccgc ttggtaggcc tgacgaatgc
      361 gtcgtcccac atctactttg ctacttaaaa tagcattcaa ctgggcctga gtttcaaaga
      421 gataagcata gtttccagct ttttctttaa tgtcagcaat agttcccgcc gcctgagcga
      481 agtgtggctt gtcctgttca ggtgtcatgt gtcggtcaag tatcggacag agaacatcct
      541 gataaaagac atagcggttg ggattgatgc cactgagatt acctggtagg tctggtaaga
      601 ggttggcaag gtcaatctgc ataaaatcct caaccgttag accagtattg gtcttgaaat
      661 gcgcagacaa actatctagg tcattgcgat agctgagttc tgcccagatt tgcaagcttg
      721 gtaggataga gaactgggca gtttcaccac cattgtctcc ccaacccatt acgatgactt
      781 ctttaatctg attggcacgg caggctttat tagcctcgat agccactaga cggctaaaat
      841 ggttgtgagg tgtaaagcca atccacttcc aagctccccc tgcaaatgca aggtcatggc
      901 taatcttgtg atgattgcgg aaattacggt tgtatttttc ctcgctatcc tgataataat
      961 cccagtaaac cagagtcaca cggtctttga gacggtctag gtagacacga gtttcctctg
     1021 gaatttccac atcacgatcg tactggccat ccgctgacat gagtttgaag aacatatcac
     1081 tccacatctg gcagtggaaa ccatatttgt cagcaatatc cagcacgcgc tccaagtgtt
     1141 ggcacatgag gagactacga tccacaacac cgttcaaaat caggtagcgt cccaaaccaa
     1201 ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt cttcagttta gacaacgtgg
     1261 caaacatgcc atcaatcagg tcataaacct tttcttcgcc aatgagaaga atgtcctcta
     1321 catcacggag ctcctgcact tccttgacac cccatttgac aaaggccgac aagtgggcca
     1381 aggtctggat gcatggtaca aaggtcatgt caaactgttg agcataggct tcgatttcct
     1441 gcaactcctc tgctgaataa gctccacgga aatagccaaa gtaaggctgt ccttcaatct
     1501 ggtaagtgtc ttccatgtaa agctcaaagg ttgagtagcc catgagagcc aagacctcaa
     1561 tcatctgctt ggcagaagcc acattcagca ccgcatttcg agaacagtca accatgtaag
     1621 ccaaatcttc gtaagctgct tgttcctcaa tctctacttt atcagcttct gctagaactg
     1681 ttaccaacaa ggataaggca cgataaagtt ggtgaggttt gcggtaggtc aattgatagt
     1741 gaccttcctc accctcgata gagatagagg cttggtcaga ctgagtgaca gccacttcca
     1801 catctggtag agaaatgtga ccttttaaaa cctcaatagc ttgcgtttgt ttgggactaa
     1861 gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag ttgacaaggg caccgtagag
     1921 attggcatct gcgtgatagg tgcaggcctg gataactggt gcgaccgtgt attcttcgta
     1981 ggcatcgaca aagtcttcaa cagccttctt aacaccttgg ataaaatctg gattttgact
     2041 gatagagcca cccagactga tgacatctgg atcgatcaga tactggatat tgagcaagcc
     2101 ttgcgtcaga ttgcggttca tgcgctcaat ggcttcttga caaagagcat taccagctgc
     2161 ggcctcttgg taaatcttgc gaccgtccca atcagtatga ccagattttt caatcacgta
     2221 tcgtaccata ttcccagttg atgctagttg cgaccagtta ttaagttttt cagcaggggc
     2281 aagggttgtc atgtagccaa attctccacc cagaccgtgg cgacctcgat aaagtctacc
     2341 attgataatc atggctccgc caatccctgt cccaatcacg acacaggctg cattttcaag
     2401 ctctggatga gctagtagtt cactgagtcc aacgcagttg gcatcatttt ctaaatggac
     2461 aggtagctga taagagctaa gcgcctcata ccaagaaaag ccatggatgt agggcaccgc
     2521 actgaagcca tcaatcacac ctgtctcttg attgaccgca cctggaacgc tcatagcaat
     2581 cccactgtaa tcctgttctg acaagcgttg atctagccac gctagtaaat cctccaagtt
     2641 ttcaggcgtt gaaatacttg tcttatccag tattttccca tcaggagtca gactggcaaa
     2701 cttaatccca gtccctccga tatcaatcgt tgcaatggtc attgttttta ccataaaaag
     2761 gggcgaatca gcagttagtt cttacttttt cgcccctcct ttcttttgat gtttactttt
     2821 tgaaattaaa tttcttcttt tttgatgaat tctgtacgaa tctcttgtgg agccaatagt
     2881 cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa tggtttgttg atgttctgat
     2941 atgcgcacat tttcttgact catattatag taacggagga catagccttc ttcattttcc
     3001 gccactttaa aggctgttgg acaaacttgc ggtatgctga gaacagaatg gctcaagagg
     3061 ctaccagtcg caaccacgct tccttcctgt ctagcaagct gaaggctggt aaacggtgtc
     3121 tgcaaggctt tggcacgacg ataggctgag aagcgttctt gggcttggtg gcattcaatc
     3181 gcaaattcga cttcaaactc acgcaagcat tgtgcttctg gcgttgggaa gtagccccag
     3241 tcacctagct cacctgatgc acgcaaaatg gtcacggcaa tggtgttatc cccaaggatt
     3301 tcgtattcat tcaatccctt gttggataca gtcacccctt tttcatcgtc atacagactg
     3361 acaaaagctt gttggtgttg aggattttca ggattttccc atgaagcagc tggtttgttt
     3421 ggtcgtgtca ccacctcata gatactttca gaatcattgc ttggacgcgt gttatgagtc
     3481 ttgaccaaga gacggatacg gtgatccttg gcagtgttag taaagcgagt cttgaagcgg
     3541 atttgtggat tgtcaacaaa gacaatcaac tcagtttcca gaggaatgtt tgtcaattct
     3601 tctgaccgtc cagcctcacg cttcataaac tcgatgatac cttgttgctc ttcttctagc
     3661 ttttcatccg cactgacagg cacggtcaat tcatgtttga gcaagatttt agcatagcga
     3721 tctgtgtttt ccaagacctc gtggccctta agctctgcaa agattggctc tgttcctttt
     3781 ggttggaaat agatatactc gtttccgatg tcaccccggt cttcgaagcg gataaaatct
     3841 tcataggctt cgtgagttgt cttatcatag actgtgatgt tgtcatccac actcaccgtt
     3901 acgaatggtg tatcaatcac tccgttttgg taaataccct cacggtgttc ttgttctcct
     3961 tccagcaatt ggaaggttgt ccaagaaagc ggtgctaggt gaactggaat ggtcacgcgc
     4021 acttgtcgag caatacgagc ttggcggaac ttatcttttg gtaaatcgta ttcaaaatta
     4081 gctccgaggt cttcgattgt aacctctaca ggacgaccat ccaagtcctc cacacggtaa
     4141 cttggcaaga taagagcagc catctttttg tagccttctg ttgggtgcaa ttccttgaaa
     4201 tcacaagtcg ccacatcaat cactgtgctg acagtatcga ccttatcatg caagcctgtg
     4261 ttaatgacag taaagagata gtcactttga gccttatccg tagcaatttt acccttccac
     4321 tcgttgagca aattactttt aacaaagttt cctacttggt tgaccttggc aaaacgcgtt
     4381 tccatctcgc ggtgaacttc gtccacgcta cagccacaga tactatcatg tggcgcattc
     4441 tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt ggttgtgtcc accagtgata
     4501 atagtcaagg gttctaccac ttgctctagg aggttgctat tttcttggaa ggcttgtttt
     4561 aggtaaatgc gggatgaaga agtgttggca agtgtgtacc agccatctgt ttcctgactg
     4621 gtcaactcgc ctgtaacagt tgataagtgt tcaggaagcg caccttctac agcttgaaca
     4681 tattcatcaa aagaactatg aacaaagatt acatccggga agagttcatt tgccacacga
     4741 atggcttcgc tcagattttt ctgtacaggc tggtggtcac agccgttcat catcaaccat
     4801 tggttggtcg aagcgtaggc acgcacatct gacaattttt gtttccagaa ggtcaaggcc
     4861 tcatctttgt caactgggat ttcattccca ttactgtacc agttggcaaa gagaatacct
     4921 aaaacacgac taccatccac accctgccag tacatttctg aaaactggga tgtaaactgc
     4981 tcatcttcaa ggacttggtt gtcaaatcca atcggcttca caccacgacc aaaggccgcc
     5041 acgtgaatgc ctgatttttg aagaatttga ggcgcttgtc ccatatttcc aaaggtatct
     5101 ggaaagtagc caatctgggt tgatttaccc catttggcag cttcttgttg accaatcaag
     5161 gtattgcgga cattggcttc actggagatc aagtagtcat cctgcaagat gtaaaaggga
     5221 ccaattttaa gtttgccctc gtcaatgtag cgttggacct tgtcgcgatt ttcagggcga
     5281 atttgtaagt agtcatcaag gacaatagtt tgtccatcca agtggaaact cttgaactca
     5341 gggtcatttt caaagagatc aaagagattg tcaaacaatt ccaccaactg catacgatgg
     5401 ctttcaaaag gcaagtacca ctcacggtcc cagtgactat gtgagataat atgtacaaca
     5461 acattttcca tgaagtaaaa cctcattcta aatttaaatt ttaacgtttt aaatgtaaac
     5521 ttgtgattct gataagaatc ggttgagcta aagcgagccc cttagcgaat atccaagtaa
     5581 tccaagacca actcacagaa catcatgtta gcccaggaga accattcacg agagtagagg
     5641 gtcggatcat ctacgtggaa gctttcgtgc atgacacctg taccaccatc gcaggcaacc
     5701 agctgatcca gcaagaattt tttctctgcc ttatctcttg ttgtcaatcc ttggatagaa
     5761 agggcgattg gccagatata gcgatagaag gtatgagaac ttccgagacc gctagcgtat
     5821 tctccttggt agaagtatgg attttcagag ctcagaatgg tacggcgagt ggcttgatac
     5881 acttcatcat cgatcgaaca gtagcccaga tagggcgcag ctagtagact tggtacattt
     5941 ggatcatcca tgatgctggc atttcctagg ccatctactt caaaagcgta aatcttttcg
     6001 cccttgctgt tggtggtgta agcgtagttt tcgattcctt cttggatttc atcctgaaga
     6061 cgcttggcat cagcaataac actctggcta tcagctaggt ttaatgctgc gaagatttct
     6121 tgcacataac ccaagactac tacagcaaac atatttgacg gaatcaagta actatactgg
     6181 caacagtcat cactcggacg aaaggctgac caagtcatac ctgtcactgc aaagtcaggt
     6241 ccaaagccat catttaccaa ggtgtcttcc ttacggtccg tatctcggac aaaacgataa
     6301 ggagagttct tgtggtcttg ttcaactgtc catagatgga gaatttcctt agtcgctgcg
     6361 acaaaagtct catcaaactg actagtctcg ccagtttctt tccagaggag ataagccaac
     6421 tgcaaaggat agcaaagcga gtccacctca tacttgcgtt cccaaatcca gccattaagg
     6481 tcggtgtggt cggtctcgtg gtgccccttc cagttttcct ctatgttgaa ggaattggca
     6541 taaggatcct tgagtaccaa ggtcatctga cgtttgacca aacctgcaat ggtctgacgc
     6601 aggagggcat ctcttttagc tacatgcagg tagggtctga gttgggctgt cgaatcccga
     6661 agccacatag caggaatatc cccagtcaag acaaaagttg aaccatcttc taagatttca
     6721 accgtattgt ccaaggtatc ggtgtagcaa cgctcgaaaa catccaccca ctctggatgg
     6781 tccttagccc gctctgctac ttcatctagc cattctctaa caatttcttt cggataaatc
     6841 atcgtgcaat tccctctttc aaacaagttt catgttcagt atatagcttt tgagacagaa
     6901 aatacataca caaatgatag tcatttttct acaaaatagt tatttttgaa actccttttc
     6961 taaaggccct ctttttctga tataatgtag aaaaacagct aaaggaaaga ctatgaaacc
     7021 actacttgaa accatcgata cccgctttgg tactgctagc aagcatgcct tctcttgggg
     7081 aaataccctg ccatacacag gcgtgccttt tgggatgaat tactttgtgc cccagaccag
     7141 tgaccaggat ggatcttggt tttttgatcc gcatctgcct atctttcagg ggattcgatt
     7201 aactcaccag cccagtcctt ggattgggga ctactcttgg ctccttctga cacctgtcac
     7261 cagtcaactg gggggagaca gcctctttca ccgccagtct tcttatgata tagataaagc
     7321 ttgcttccag cctcactatt tgaagctctt ttctctgcgt tatcagattg aaacccagct
     7381 cacaccaact tgctatggtg cttctattcg tttgaatcaa aagcaaggca aagccctctc
     7441 cctctatctt cacgcagcag atgaactgac agtagagcaa gtagataagc ggactcttgc
     7501 cctgcgacaa gaaggtaaaa ctgaaactaa caaaaattcg ctaacgatgt tcactgccct
     7561 gcaaatgaac acggatattc ttgctatcag ccaagaagct ggagactggc gaattgactt
     7621 agtaagtagt caaaccgaga tgcaactagc cacttctttc atctctcctt ctcaagctct
     7681 aatcaatcta cctcaagaag attttgatag ctgtaaatca agtgcccaag cggattggga
     7741 aaatctcctc catcgttttg acattataga gacaggagag gctgaccgaa ccttctttga
     7801 ccactgcctc tacagactct tcctcttccc acagactttt tatgaggttg atgagtcagg
     7861 gcaagccatc cacatggatc tggctactgg tactgtcaag cctggtgtcc tctttagcaa
     7921 caatggtttc tgggatacct tccgcaccac cttccccctc tttgccctta tcataccgga
     7981 gcactatcaa cgctttttag aaggtttcct caatagctac cgcgatactg gtttccttcc
     8041 aaaatggctg gctccagatg aacggggtat gatgccaggt acacttttag acggtattat
     8101 cgcagatagc gcctgcaagg acatggcccc tgacctagaa ggagaactct tccaagccat
     8161 gctcgaaaca gccagcaaag ccgaccctct cggcatcaat ggccgccacg gactagccca
     8221 ataccaagaa ctaggttacc tctctaccga ccaccatgaa agtgttagcc atactctaga
     8281 ctatgcctat agtgactttt gtatcgccag ctgtgccaaa aaactagaga acatagaaat
     8341 cgctgaaacc tacaaggctg cttcacaaaa ttaccgccag ctatttgacg ctgagacagg
     8401 ttacatgcga gcacgagaca atcaaggaaa ctttcaccct gacttctctc cttatagttg
     8461 ggggcgagac tacgctgaat gctctgccat tcaagctact ttaggcgttc tccacgacat
     8521 ccctggctta atccaactga tgggtggaaa agaaaccttt agcaactatc ttttgaaagc
     8581 ctgtcaagat gctcccctct ttgaaacaac aggctatggt tacgaaattc acgaaatgag
     8641 cgagatggct actgctcctt ttggacaaat agccatttcc aaccagccta gtttccacat
     8701 tccttatctc ttccgctaca gcgattaccc tgactacact gcccttctta tcaagacgct
     8761 ccgtcagaaa gcttttcacc caagttggga agcctatcct ggagatgaag acaatggtag
     8821 tctctctgct tggtacatct ggtcagctct cggattttat ccaacctgtc caggaaaacc
     8881 aagctatgac ctcggaatcc ctctctttga ccatctacga gtctacctag ctaaagaaga
     8941 taaatggctg gatatccata ctaaacaaaa ccacaaccat tttaactttg tcaaagaatg
     9001 ccgactggac aaaatactcg tatcaactat tcaacaccaa gacctcttaa aagctgaaca
     9061 actaactttc accctcagct ggttaccaag tcactaataa ccaaaagaac ctttgcatcg
     9121 cgcaaaggtt cttcttttat gaaacttgga cttgaagttg atccacaagc tgaccatcca
     9181 ccgttaggtt cagatagaaa tacaaggttt tatctcctgc gaaatacaag gttggtagcg
     9241 tcagactttt ttcagtctca ccagcttgaa acgcaaggac ttgaatctca tcctgatagg
     9301 cgacaccatg gacacctgtc cccggttgaa tcgaaatctt agcttctaaa ggaccactac
     9361 attctctgcg cttaactgta aaataggcat tttctccctt tgccacagct aggctttttt
     9421 ctgcaaatgc tagttcctga acaacttctt tttttgataa tccaggagtt ttataaaggg
     9481 aaatcttggt caatacagga atagattgtg cctcggtaat catcacgcgc accttctgtg
     9541 cctctactag cggacctcgc aagaggcgct tatgaccaac tgtgaagccc ctgccaaatt
     9601 cctgccagac accatccacc tctacttgca catgaaaagc agcgattcgt tgccctagct
     9661 tcaaatcttc tcttaactca attacatcaa aagttttagg agaacctaag tcgagttcta
     9721 actggatggg caagtctgca tcgtttgccc aagagctggt ctcaaggccg tctgtcaaat
     9781 ggcgacaagc aaagtctgcg gaaagagctg gaccagatac ctcagctccc agagccaaat
     9841 cttctttata gagctcattg cgataggtcg caaattcata aagtcgttca atatcctttg
     9901 catcaaagag cccagcttga ttcggcggaa tattaagcaa gagtggagtt cctcgcccta
     9961 ctgagtgaaa gtagatttcg accaactcct cgagagactt aggatcctga tcctcatggt
    10021 agaaccagcc tggacggatg gaaacatctg cctctccgat tgaaaaaatc gtgcccgagg
    10081 gatccccgtg ctgaagatag tccagctctg cttctgttcc tagtttatca ggattcacct
    10141 tttgccacag tggatcacct gcataccctc gttcattgcc aatccagcgg atactggtgc
    10201 cttctgttga aaaaatcaaa caatcgccct gcaagtcacg aatggtttca aaccattttt
    10261 caaattcata attgacctgc tgggcccctt cccctctagc accatccatc caaacctcag
    10321 cgaacttacc agcattccca tagttaggat ttgataagat ttccttcaac tgagccagat
    10381 aataggcatt gtagtccgct tctcggtcca catgatagag gggactatgg gcatcccacg
    10441 gtgacaggta gacccccata tccatatcaa actctgtggc agcttgggat acttcaagga
    10501 gcaagtcgcc ctttcctctc ctccaaggac tgaccttaac cgaataatct gtgtgagctg
    10561 tcggataaag gacaaagcca tcgtggtgct tgaccaccaa aatcaacttt ttgaagcccg
    10621 tttccttgag cacacgaacc cactcacgcg catccaactg actcgggtta aagcgctcag
    10681 gatcctcctg acctgttccc cattcctgat tataaaaagt attaggtcca aaatggataa
    10741 aggctgctag ttcatctccc agataagcta gctgagtctg acttggtaac ggtccatgcg
    10801 gtttgatttt cttcatcgtt atatatctcc ttcttttgca acttctcttg ttcacaaggt
    10861 tatccacagg ttgtggataa taaaccatga tctaacagcc agctctttcc aactccaact
    10921 ggtaggctca ggatttttct cctcaatatt ccctcttcaa acagctctgt catatggttc
    10981 tgctaacacc tctcagaatg ctactctcca taattattct caggcttctt tcaggtcatt
    11041 gtgtgaagac cattcactct ccagactttt ccacagattg tgaataagtg attattctaa
    11101 actatcacct cgtcgactat aacaaaaaat aaaaagaggg tcaataagat aattatctct
    11161 tcctaaatac tcagaaaaga ttctataatt tgtacaattg aacaagaaat gcttctccaa
    11221 aatctatctt caaaagaatg ttgcatcttt ttatcctttc acacaaacat ttctctttat
    11281 acagatccaa gtgataacca gatgataatt ggacactttt tggttatttg tgtatttttt
    11341 gaaaaatttt ttaaaaaaat tttatatcct ctttaataga agtttatgca tttgtatata
    11401 tgtattccat cctatgttgt gtataataac ataaaattgt gctaaatatc ataatttata
    11461 ttattttccg agaaattata gccattgaca ttacctagga attgcgttat aacatacatg
    11521 taagcggtac ccaaaatggt gccaagtcaa aatttttaag gaggaaaata catgtcttca
    11581 catccaattc aggtcttctc agaaattggg aaactgaaaa aagttatgtt gcaccgtcca
    11641 ggcaaggagt tagaaaactt gttgccggac tatcttgaaa ggcttctttt tgatgatatt
    11701 cctttcttgg aagatgctca aaaagaacat gatgcatttg cccaagctct tcgcgatgaa
    11761 ggaattgagg ttctctacct agaacaactc gctgctgaat cattgacctc tccagaaatc
    11821 cgcgatcaat ttatcgagga atacttagac gaagccaaca tccgtgatcg tcaaactaag
    11881 gttgctattc gtgaattgct tcacggcatc aaggacaacc aagaattggt tgaaaaaaca
    11941 atggctggga ttcaaaaagt tgaattgcca gaaattcctg acgaagctaa agatctaact
    12001 gacttagttg aatcagatta tccatttgca attgacccga tgccaaacct ctatttcact
    12061 cgcgacccat ttgcaacaat tggaaacgcc gtatcgctta accacatgtt tgcagacact
    12121 cgtaaccgtg aaacactcta cggtaagtat atcttcaaat accacccaat ctatggcgga
    12181 aaagtggatt tggtctacaa ccgtgaagaa gatacgcgta tcgaaggtgg agacgagcta
    12241 gttctttcta aagacgtcct tgcagtaggt atctctcaac gtacagacgc agcttctatc
    12301 gaaaaacttt tggtcaacat cttcaagaaa aatgttggct tcaagaaagt tttggccttt
    12361 gaatttgcta acaaccgtaa attcatgcac ttggatactg tcttcactat ggtagactat
    12421 gacaagttca ctattcaccc agaaatcgaa ggcgaccttc acgtttactc agttacttac
    12481 gaaaacgaaa aacttaaaat tgttgaagag aaaggtgact tagctgaact tcttgctcaa
    12541 aaccttggtg tagaaaaagt tcatttgatt cgttgcggtg gtggcaatat cgtagcagct
    12601 gcgcgtgaac aatggaacga cggttctaac actttgacca tcgcacctgg tgtggtagtt
    12661 gtttatgacc gcaataccgt gaccaataag attttggaag aatacgggct tcgcttgatt
    12721 aagattcgcg gaagtgaatt ggttcggggc cgtggtggac ctcgttgtat gtctatgcca
    12781 tttgaacgtg aagaagtgta atcgctgttc ggtattcgtc aatagaaaat gtaaaaaata
    12841 gaaagaggaa ataataaaat gacaaattca gtattccaag gacgcagctt cttagcagaa
    12901 aaagacttta cccgtgcaga gttagaatac cttattggtc tttcagctca cttgaaagat
    12961 ttgaaaaaac gcaatattca acaccactac cttgctggca agaatatcgc tctcctattt
    13021 gaaaaaacat ctactcgtac tcgtgcagcc tttacaactg cggctatcga ccttggtgct
    13081 cacccagaat acctcggagc aaatgatatt cagttgggta aaaaagaatc tactgaagat
    13141 actgctaaag tattgggacg tatgtttgac gggattgaat tccgcggatt cagccaacgt
    13201 atggttgaag aattggcaga attctcaggc gttccagtat ggaacggtct aactgacgaa
    13261 tggcacccaa ctcaaatgct cgctgactac ttgactgttc aagaaaactt cggtcgcttg
    13321 gaaggcttga cattggtata ctgtggtgat ggacgtaaca atgttgccaa cagcttgctc
    13381 gtaacaggtg ctatccttgg tgtcaatgtt cacatcttct caccaaaaga actcttccca
    13441 gaaaaagaaa tcgttgaatt ggcagaagga tttgctaaag aaagtggcgc acatgttctc
    13501 atcactgaag atgctgatga agcagttaaa gatgcagacg ttctttacac agacgtttgg
    13561 gtatcaatgg gtgaagaaga caaattcgca gaacgtgtag ctcttcttaa accttaccaa
    13621 gtcaatatgg acttagttaa aaaagcaggc aatgaaaact tgatcttcct acactgcttg
    13681 ccagcattcc acgatactca cactgtttat ggtaaagacg ttgctgaaaa atttggtgta
    13741 gaagaaatgg aagtaacaga cgaagtcttc cgcagcaagt acgctcgcca cttcgatcaa
    13801 gcagaaaacc gtatgcacac tatcaaagct gttatggctg ctacacttgg taacctttat
    13861 attcctaaag tataatttta gataataaac cgtctaccaa cagctatgag ggctgcgact
    13921 aatagcttta gtccggtcct cttttatata atggtaatct attatttctt ataaaatatg
    13981 tgaaaaatca ttaaattgaa atctaaacgc attctattga gtgtgataaa ggagaattta
    14041 tggcaaatcg taaaattgta gtagctttgg gaggaaatgc gattctttct tctgacccat
    14101 cagcaaaggc tcaacaagaa gctttagttg aaacagctaa gcatcttgta aaattgatta
    14161 aaaatggaga tgatctgatt atcactcacg gtaatggacc tcaagttggg aatctcttgc
    14221 tccaacattt ggcatcagac tctgaaaaga accctgcctt cccactcgac tcacttgtcg
    14281 ctatgacaga aggtagcatc ggtttctggt tgaaaaatgc tttgcaaaat gctctcttgg
    14341 atgaaggcat cgaaaaaaat gttgcctctg ttgtaacgca agttgtcgta gataaaaatg
    14401 atccagcttt tgttaacttg agtaaaccaa tcggtccttt ctattcagaa gaagaagcaa
    14461 aagcagaagc cgaaaagagc ggagcgactt tcaaggaaga tgctggccgt ggctggcgta
    14521 aggtcgttgc ctcaccaaaa cctgttgaca tcaaagaaat tgaaaccatc cgtactcttt
    14581 taaataatgg tcaagtcgtc gtagctgcag gtggtggcgg tattcccgtc gtcaaagaaa
    14641 acaatggaca tttgactggt gtcgaagcgg ttattgataa agacttcgct tcccaacgtt
    14701 tggcagaatt ggttgatgca gacctcttca tcgttttgac aggtgtagat tatgtatttg
    14761 ttaactacaa caagccaaac caggaaaaat tggaacatgt gaatgttgcc cagctggaag
    14821 aatatatcaa acaagatcag tttgcaccag gtagcatgct tccaaaagta gaagcagcta
    14881 tcgcttttgt caatggtcgt ccagaaggaa aagcagttat tacttccctt gaaaatctag
    14941 gcgccttgat tgaatctgaa agcggaacaa ttattgaaaa aggataagtt gttttactaa
    15001 taagatgtat tctatttcta gtatctttat atcaaattag aaattattct tgaaaacatg
    15061 tacaatattt caaaagatat tagtttttta gactttaata tggtaaaaca aatataaata
    15121 gaaagcgttt tcttaaatgt ttatttaaga aagtagttgg ttttttacac tttgttagac
    15181 atcaggagga aaaacaaatg agtgaaaaag ctaaaaaagg gtttaagatg ccttcatctt
    15241 acaccgtatt attgataatc attgctatta tggcagtgct aacttggttt atccctgcgg
    15301 gggcctttat agaaggtatt tacgaggctc agcctcaaaa tccacaaggg atttgggatg
    15361 tcctcatggc accgattcgg gctatgctag gtactcatcc agaggaaggt tcgctcatta
    15421 aagaaacgag cgcagcgatt gatgtagcct tcttcatcct tatggttggt ggtttccttg
    15481 gcattgtcaa caaaactggt gctcttgacg tagggattgc ctctatcgtg aagaagtata
    15541 agggccgcga aaaaatgtta attttggtac tgatgccttt gtttgccctc ggtggtacaa
    15601 cttatggtat gggggaagaa acaatggcct tctatccact ccttgtgcca gttatgatgg
    15661 ccgttggttt tgatagcctg actggtgttg caattatttt gctcggttct caaatcggct
    15721 gtttggcatc tactctgaat ccatttgcga caggtattgc ttcagcgact gcgggagttg
    15781 gtacagggga cggtatcgta cttcgtctga ttttctgggt taccttgact gctcttagta
    15841 cttggtttgt ttaccgttat gcggataaga ttcaaaaaga tccgactaag tcactggttt
    15901 atagtactcg caaagaagat ttgaaacact ttaacgtaga agaatcttca tctgtagaat
    15961 ctacacttag cagcaaacaa aaatcagttc tcttcttatt tgtgttgaca ttcatcttga
    16021 tggtattgag cttcattcca tggacagacc ttggcgttac catttttgat gactttaatg
    16081 cttggttgac tggtcttcca gttattggta atattgtcgg ttcatctact tctgcactag
    16141 gtacttggta cttcccagaa ggcgccatgc tctttgcctt tatgggtatc ctgattggtg
    16201 ttatttatgg tcttaaagaa gataagatta tctcttcctt catgaatggt gctgctgact
    16261 tgctcagtgt tgccttgatc gtagcgattg ctcgtggtat tcaagttatc atgaacgacg
    16321 gtatgattac cgatacaatc ctcaactggg gtaaagaagg cttgagcggt ctatcttcac
    16381 aagtctttat cgttgtaact tatatcttct atctacctat gtcattcttg atcccatctt
    16441 catctggtct tgccagcgca actatgggta tcatggctcc acttggagaa tttgtaaatg
    16501 tccgtcctag cttgattatc actgcttacc aatctgcttc aggtgtcttg aacttgattg
    16561 caccaacatc tggtattgtg atgggagctc ttgcacttgg acgtatcaac attggtactt
    16621 ggtggaaatt catgggcaaa ctcgtagtcg ctattattgt agtgaccatc gcccttcttc
    16681 tccttggaac cttccttcca ttcctataaa atagtgagtg aggtgattcc atgaaaatag
    16741 atataacaaa tcaagttaaa gatgaatttc ttatatcatt aaaaaccttg atttcctatc
    16801 cttcagtact caatgaagga gaaaatggaa caccttttgg acaagcaatc caagatgtcc
    16861 tagaaaaaac tttagagatt tgtcgagaca taggtttcac tacctatctt gaccctaaag
    16921 gttattacgg atatgcagaa atcggtcagg gagcagagct tctggccatt ctctgtcatt
    16981 tggatgttgt tccatcaggt gatgaagcag attggcagac accgccattt gaagcaacta
    17041 tcaaagacgg ctgggtattc ggacgtggtg tccaagatga taaaggccct tcgctcgcag
    17101 ctctctatgc agtaaaaagc ttgctggacc aaggtattca gttcaaaaag cgcgtacgct
    17161 ttatctttgg taccgatgag gaaaccctct ggcgctgcat ggcacgctac aataccatcg
    17221 aagaacaggc cagtatgggc tttgcacctg actcatcttt tcctctgacc tatgctgaaa
    17281 aagggcttct acaggtcaaa cttcatggcc cgggatcgga tcaactagag cttgaagtag
    17341 gaggcgcctt taacgttgta ccagacaagg ccaactacca aggtctcctc tatgaacagg
    17401 tttgtaacgg tctcaaagaa gctggttatg attaccaaac cactgaacaa accgtaacgg
    17461 ttctcggagt gccaaagcat gctaaggatg ctagtcaagg tatcaatgct gtcatccgac
    17521 tagctaccat tcttgctcct ctccaagaac accctgctct cagttttctt gcaacacaag
    17581 caggtcaaga cggcacagga agacaaatct ttggtgatat agcagatgaa ccttctggtc
    17641 acctatcctt taatgtcgca ggtctcatga tcaatcatga acgttctgaa atccgtattg
    17701 acattcggac tcctgtctta gctgacaagg aagaactagt agagttgctt acaagatgtg
    17761 cacaaaacta ccaactccgc tacgaagagt ttgactatct agcgcctcta tacgtcgcaa
    17821 aagacagtaa actcgttagc acactgatgc aaatctacca agaaaagact ggcgataaca
    17881 gtcctgctat ttcatccggt ggtgccactt ttgctcgcac catgccaaat tgtgtagcct
    17941 tcggcgcctt attcccagga gcgaagcaga cagaacatca ggcaaatgaa tgtgccgttc
    18001 tagaagattt gtaccgtgct atggatattt atgccgaagc cgtctatcga cttgcaactt
    18061 aatcaggcaa ctgtttctac aaaaaaaatc gaccgattaa tgaactgcac cccaaaagtt
    18121 agacagaa
//
LOCUS       DBERYS010000039        17298 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.039, whole genome shotgun sequence.
ACCESSION   DBERYS010000039 DBERYS010000000
VERSION     DBERYS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.039"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            168..857
                     /locus_tag="ACL8LA_001540"
     CDS             168..857
                     /locus_tag="ACL8LA_001540"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS1813281.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPK
                     EIAQRFINVIK"
     gene            882..1799
                     /locus_tag="ACL8LA_001541"
     CDS             882..1799
                     /locus_tag="ACL8LA_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGS1813282.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            1827..3368
                     /locus_tag="ACL8LA_001542"
     CDS             1827..3368
                     /locus_tag="ACL8LA_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGS1813283.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAIAIVYSYTGGLKSVLWTDFIQGVVLISGVVVALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLAIATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGVTGL
                     ILAAIYAAAQSTVSTGLNSVATSWTLDIQDVISKNMSEDRRTKIAQFVSLAVGLFSIV
                     VSIIMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTENKVSAPKYTTIHDISEIKADSS
                     WEVRH"
     gene            3394..3852
                     /locus_tag="ACL8LA_001543"
     CDS             3394..3852
                     /locus_tag="ACL8LA_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1813284.1"
                     /translation="MIISEQSDFRRYASVNKHFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            3854..6076
                     /locus_tag="ACL8LA_001544"
     CDS             3854..6076
                     /locus_tag="ACL8LA_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HGS1813285.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTSISEVQHKSGNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVTRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPAPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIREHGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTQVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQISIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6116..7225
                     /locus_tag="ACL8LA_001545"
     CDS             6116..7225
                     /locus_tag="ACL8LA_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1813286.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            7382..8272
                     /locus_tag="ACL8LA_001546"
     CDS             7382..8272
                     /locus_tag="ACL8LA_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1813287.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWEGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLSSGDKVAKETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNVVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            8297..8620
                     /locus_tag="ACL8LA_001547"
     CDS             8297..8620
                     /locus_tag="ACL8LA_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813288.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELISIESNLEMKQQNLIDEVNKTIKENDAKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            8610..10601
                     /locus_tag="ACL8LA_001548"
     CDS             8610..10601
                     /locus_tag="ACL8LA_001548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HGS1813289.1"
                     /translation="MAISQMKRISLLFTKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFSTIQISHTSNSHHGVIEGNDALNYLMNKQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERNIQSNNEEIERLIKW
                     EKLEIIPAHLEQFSFCRGKVGTIPRTVDNRLYNNFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGIMLALKIFHLPSVTKKFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ITTTSDVMTILVVSVVFGFITVFVGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDIRPLFVLGKWVSIFNAVGILIASIIQAKSLSGIGSGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            10617..11093
                     /locus_tag="ACL8LA_001549"
     CDS             10617..11093
                     /locus_tag="ACL8LA_001549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HGS1813290.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11127..11708
                     /locus_tag="ACL8LA_001550"
     CDS             11127..11708
                     /locus_tag="ACL8LA_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813291.1"
                     /translation="MSNLENLRESVIEQAHEKGSMKLLDSKKKIDDEFEMQKSVIVKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            11773..12780
                     /locus_tag="ACL8LA_001551"
     CDS             11773..12780
                     /locus_tag="ACL8LA_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HGS1813292.1"
                     /translation="MDTNLFSKINTTISVKENDFITEEKFQKIIQSKDTETLAFILEA
                     TPYHLSIDVLENPSQTEISLMTKLVNDYRWAYAESPSDKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELGEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKTLDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYG
                     S"
     gene            12770..13090
                     /locus_tag="ACL8LA_001552"
     CDS             12770..13090
                     /locus_tag="ACL8LA_001552"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HGS1813293.1"
                     /translation="MEANKYKIGIIGSHDIILPFSMIGFDIFSAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            13153..14928
                     /locus_tag="ACL8LA_001553"
     CDS             13153..14928
                     /locus_tag="ACL8LA_001553"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HGS1813294.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            14929..16314
                     /locus_tag="ACL8LA_001554"
     CDS             14929..16314
                     /locus_tag="ACL8LA_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HGS1813295.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            16337..16948
                     /locus_tag="ACL8LA_001555"
     CDS             16337..16948
                     /locus_tag="ACL8LA_001555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HGS1813296.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            16990..>17298
                     /locus_tag="ACL8LA_001556"
     CDS             16990..>17298
                     /locus_tag="ACL8LA_001556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813297.1"
                     /translation="MDKALSLSKLFLLTVSGYLFQNFGLLDNENVSTNLDLGLVNRKC
                     NYIESKRVITRYLSFMELLLYTISKVKLLEDILMPQPIVPVEIPQSRRFDSKKRNDIL
                     L"
BASE COUNT         5798 a         2458 c         3470 g         5572 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa agccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgata tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccaaa agaaattgct cagcgattta
      841 ttaatgtcat caaataaaaa acaatataaa ggagaaatct gatgaaagat ttaactaaat
      901 acaaaggcgt tattcctgca ttttatgctt gctatgatga aaatggtgaa attagccaag
      961 aacgtgtaaa atctctggtg caatatttca ttgacaaagg tgtaaaaggt atctatgtaa
     1021 atggttcttc aggtgaatgt atttaccaaa gtgtagaaga tcgtaaacaa attattgaag
     1081 ctgttatgga agttgctaaa ggtaaattaa cagttatcaa ccatgttgca tgtaataaca
     1141 cgaaagatag tattgaattg gcaaaacatt cagaaagtgt tggagtcgat gctattgctg
     1201 ctatcccacc tatttacttc aaattgccag aatattcaat tgcagcatat tggaatgcaa
     1261 tgagtgaagc tgcgccaaat acagatttta tcatttataa tattccacaa ttggcagggg
     1321 ttgcgttgac tggtagtttg tatgcaacaa tgcgtcaaaa tcctcgtgtg attggagtta
     1381 aaaattcttc tatgcctgta caagatattc aaatgtttgt agctgcaggt ggagaagatt
     1441 acattgtatt caatggtcca gatgaacaat atcttggtgg tcgcttgatg ggagcagaag
     1501 ctggtattgg tggtacttat ggcgttatgc cagatttgtt cttgaaattg gaaagtttga
     1561 ttcaagaacg agatttagat actgctaaaa aacttcaata tgctatcaat gaagttatct
     1621 ataagatgat atcaggtaag gcaaatatgt atgctgtagc aaaagaggtt ttgcgtctaa
     1681 atgaaaaact tgatttaggt tctgttcgtc aacccttaga agcattggca gaaggtgact
     1741 tggaagttgc aaaacaagca gcagaactta ttcaacaagc acgaaaagaa tttttataat
     1801 aaataaaatc aatatggaga tatgttatgg gcacaacagg atttacaata attgacttaa
     1861 ttatcttgat tgtttattta cttgcggtgt tggttgcagg tatctatttc tctaaaaaag
     1921 agatgaaagg aaaagagttc tttaaaggag atggttcggt tccttggtat gttacttcgg
     1981 tatccatttt tgccacaatg ctcagtccga tttccttctt gggactcgct ggtaactctt
     2041 atgcaggtag ctggatttta tggtttgctc aattagggat ggttgtagct attccactga
     2101 caattcgttt tatcttacct atctttgcac ggatagacat cgatacggca tatgattact
     2161 tggataaacg ttttaattct aaagcacttc gtattatttc agcactcttg tttattattt
     2221 atcaattggg acgtatgtct atcattatgt acctcccatc agctggttta tcagtattga
     2281 caggaattga catcaatatt ttgattattt tgatgggtgc aattgcaatt gtttattctt
     2341 atactggtgg tctaaaatcc gtattatgga cagactttat tcaaggtgtg gttctgatta
     2401 gtggtgtcgt tgtagcttta tttgtactga tggctaatat taaaggtggc tttggtgcag
     2461 tagcagaaac attagcaaac gggaaattcc ttgctgcaaa tgaaaaactt ttcgatccta
     2521 acttgctttc aaactccatc ttcttaattg tgatgggttc aggctttaca atcttgtctt
     2581 cctatgcttc atctcaagat ttggttcaac gttttactac aacacaaaat attaagaaac
     2641 ttaataaaat gttgttcaca aatggtgtct tgtcacttgc aattgcaaca gtcttttact
     2701 tgatcggtac aggcttgtac gtattctatc aagtacaaaa tgcaggtagt gcagctagca
     2761 atatccctca agaccaaatc tttatgtact ttattgcata ccagttacca gtaggtgtca
     2821 caggtttgat cttggcagcg atttatgcag cagctcaatc aactgtttca acaggtttga
     2881 actctgttgc aacttcatgg acattggata ttcaagatgt catttctaaa aatatgtcag
     2941 aggatcgtcg tacgaaaatt gcacaattcg tatctctagc agtaggttta ttctcaattg
     3001 ttgtttccat tatcatggct cattcagata ttaaatctgc atacgaatgg ttcaatagtt
     3061 tcatgggact tgtacttggt ctacttggtg gtgtattcat tcttggtttt gtttctaaaa
     3121 aagcaaataa acaaggtgct tatgcagcgc tgattgtatc aaccatcgtt atggtattta
     3181 ttaaatactt ccttcctcca acagctgtta gctactgggc atattcattg atttcaattt
     3241 ctgtatcagt agtttcaggt tatattgtat ctgttcttac tgaaaataaa gtatctgcac
     3301 ctaaatatac aacgattcat gatatttcag aaattaaagc ggattcaagt tgggaagttc
     3361 gtcactagtc actaatagaa aagagaaaat aatatgatta tttctgaaca atctgatttt
     3421 agacgatatg cttctgtcaa taaacatttt tcaaaagtct gtgatttttt agaaaataca
     3481 aatttgacag atttagttga tggaaaaatt gatattgacg gggaaaatgt ttttgcaaat
     3541 tgtatgactt atctagctga tggagttcca ggagatattt ttgaaactca taagaaatat
     3601 ttagatattc atattgttgt tgaaaataca gaaaaaatgg cagtaacttc cccagttcgt
     3661 gctcagtcac gtgtaccttt tagcgaagag aaggatattg ctttttacga tagcaaagac
     3721 tatcaaattg ttgaattgct tccagggaat atgttggtga cttttgaaga agatcttcac
     3781 caaccaaaga ttcattgcaa tgatgaaact gttaaaaaac ttgttatcaa agttttaaac
     3841 gaggaaaaat aaaatgaaaa aaaatattaa acaatatgta accttaggta ctgtagtggt
     3901 attatcagca tttgttgcta actcagttgc agctcaggag actgaaactt ctgaagtatc
     3961 aacaccagag ttggtgcaac ctgttgcacc aacgacttcg atttcggaag tacaacataa
     4021 atcgggtaac tcttcggaag ttactgtaca acctcgaaca gttgaaacta ctgttaagga
     4081 tccatcttct acagcggaag aaactcctgt cttagaaaaa aataatgtta ctttaacagg
     4141 gggcggagaa aatgttacta atgagttaaa ggataaattt actagcggtg actttactgt
     4201 agtgattaag tacaatcagt ctagtgagaa aggtttacaa gctctgtttg gaatatctaa
     4261 ttctaaaccc ggtcaacaaa atagttatgt agatgtgttc cttagagaca atggtgaatt
     4321 gggaatggaa gcgcgtgata cttcttccaa taaaaatcac ctagtaacca gacctgcttc
     4381 agtttggggt aagtacaaac aagaggccgt gacgaacact gttgcagtag tagcagattc
     4441 agtcaaaaaa acgtattctt tatatgcaaa tggtacaaaa gtagtagaaa agaaagtgga
     4501 taacttccta aacatcaagg atattaaagg tattgattac tatatgcttg ggggagtgaa
     4561 acgtgcagga aaaacggcgt ttggttttaa cggaacacta gaaaatatca aattctttaa
     4621 tagtgcattg gatgaagaaa ctgttaaaaa gatgacaaca aacgctgtta ctggacattt
     4681 aatttatacg gctaatgata caacaggttc taactatttc cgtattccag ttctgtatac
     4741 ttttagcaat ggtcgggttt tttcaagcat tgacgctcgt tacggtggaa ctcatgattt
     4801 cttgaataaa attaatattg ctacaagtta tagtgatgat aatggtaaga catggactaa
     4861 accaaaatta acattagcat tcgatgattt tgcgccagca ccattagaat ggcctcgtga
     4921 tgttggtgga cgtgacttac aaatcagcgg tggtgcaacc tatattgact ctgttattgt
     4981 tgaaaaaaat aacaaacaag tactcatgtt tgctgatgtt atgcctgctg gagtaagttt
     5041 tagagaagct actagaaaag attcaggtta taaacaaatt gatggtaatt attaccttaa
     5101 attaaagaaa caaggtgata ctgattataa ttatactatt cgtgagcatg gtactgtata
     5161 cgacgatcgt accaacagac caactgaatt ttcagtagat aaaaatttcg gtattaaaca
     5221 aaatggtaat tatttgacgg tagagcaata ttcggtttca tttgaaaata ataaaaagac
     5281 tgaatatcgt aatgggactc aagtccatat gaatatcttc tacaaagatg cattattcaa
     5341 agtagtgcca acgaactata ttgcatatat ttctagcaat gatcatggcg aatcttggtc
     5401 tgcaccaact ttattacctc ctataatggg acttaatcgg aatgcgcctt atttgggtcc
     5461 tggacgtgga atcattgaaa gctcaactgg acgtattctt attccgtctt acactggtaa
     5521 agagtctgca ttcatttata gtgacgataa tggagcgtct tggaaagtta aagttgtgcc
     5581 acttccttct agttggtcag cagaagcaca atttgtagaa ttgagtccag gagtaattca
     5641 agcatacatg cgtacaaata atggtaaaat tgcatattta acaagtaaag atgcaggtac
     5701 tacttggagt gcgccggaat atttgaaatt tgtttcaaat ccaagttatg gaacacaatt
     5761 atcaatcatc aattatagtc aattgattga tggtaaaaag gctgtcattt taagtactcc
     5821 aaactccaca aatggtcgta aacacggaca aatttcgatt ggtctaatta atgatgataa
     5881 tacaattgat tggcgttatc atcacgatgt tgattatagt aactatggat actcatattc
     5941 aacattgaca gagttaccaa atcatgaaat tggattgatg tttgaaaaat ttgattcatg
     6001 gtctcgtaat gaacttcata tgaaaaatgt tgtaccatat ataacattta agattgaaga
     6061 tttgaaaaag aattaaagct gaaatttaaa aatatataga aagaggataa aaattatggt
     6121 aaattacggt attgttggag ctggatattt tggagctgat ttggctcgct caatgaacaa
     6181 aattgaagat gcaaaagtgg ttgcggtatt tgacccaaat catggagaag aagttgctca
     6241 agagttggga tcagatgttt gtgcaagttt agatgaactt gtagcacgtg aagatattga
     6301 ttgtgtgatc gtagcttcac ctagctacct tcaccgtgaa cctgttgtga aagctgctca
     6361 acatggcaaa cacgtatttt gtgaaaagcc aattgcattg tcttatgaag attgtaaagc
     6421 catggttgac gcatgtaaag aaaataatgt tatctttatg gctggtcaca tcatgaactt
     6481 ctttaatggt gtacaccatg ctaaagaatt gattactcaa ggtaaaatcg gtaaagttct
     6541 ttattgccat gccgctcgta caggttggga agaacaacaa ccaactgtat catggaagaa
     6601 acttcgttct caatctggag gacatttgta ccaccatatt catgaattag attgcattca
     6661 gttcatcatg ggaggacttc ctgaaaaagc gacaatggta ggaggcaatg tatatcataa
     6721 aggtgaaaac tttggtgatg aagatgatat gctcattgta aacctagaat actctgatga
     6781 tcgttatgct gttttggaat atggtaatgc tttccgttgg ggtgaacact acgtcttgat
     6841 tcaaggaact gaaggagcta tcaaacttga cttgttcaat actggaggta ctcttcgtgt
     6901 taaaggtgaa ggagaatcac acttcttagt acatgaaact caagaggaag atgatgaccg
     6961 tacagctatc tataccggtc gtggtatgga tggagcaatt gcgtacggta aaccaggagt
     7021 acgttgccca ttatggttac aaacatgtat tgataaagaa atggaatatc tacatgacat
     7081 cattaaaggt ggagaaatta cagaagaata tgaaaaactt ctcaatggtg tagctgcttt
     7141 agaatcaatc gctaccgctg atgcatgtac tttatcagtt aaagaagatc gaaaagtaag
     7201 tctttcagaa atcacaaatg cttaactatt gtaaaacaga atagtaaatt cttgtcatta
     7261 tataatttct aaagttctgt gatacaactc attgaataaa gaaatagaga tgggactggg
     7321 ataatgccca gtcccatttt ttatcaaaaa gtaatgagat caaaaatgtg ggagtgatga
     7381 aatgaagatt atagggatcg atattggcgg aacaacaatt aaggcagatt tatacgatga
     7441 ttttggaacg agtttaaatc agttcaaaga gatagaaaca attattgact atgatttggg
     7501 aacgaaccag atattaaatc aggtctgtga tttaattggt gagtatgttt taaattattc
     7561 aattgatggt gttgggattt ccactgctgg agttgttaat gctaatacag gagaaatcat
     7621 ctatgcaggc tatacaatac cagggtatat cggagtaaac tttacttccg aaatagaaaa
     7681 acgttttggg ttgtctactt ttgttgaaaa tgatgttaat tgtgctgcat taggtgaatt
     7741 gtgggaggga caagccaaag ataagaaaaa tgtagtaatg gttactattg gaacaggtat
     7801 aggaggcagt attattgtca atggacaaat tgttaacgga tttaactata ctgctggtga
     7861 agtaggctat attcctgtag gtaattcgga ttggcaaagc aaagcctcaa caaccgcatt
     7921 gattcattta tatcaaaaga agagcttgaa aactaatcaa actggacgta ctttctttac
     7981 tgatttaagt tctggagata aagtcgctaa agaaactttt gaaatttttg tagaaaatct
     8041 aacaaaaggt ttattaacta tttcttatct acttaatcca gaaattctca tattaggagg
     8101 tgggattctg gctaaaaagg atattttgtt acctgaaatt caaagttctt tagctaaaaa
     8161 tgcaatggat aataggtttt tgcctaaaaa tgttgtggca gctacattag gaaatgaagc
     8221 tggtcgtata ggagctgtaa aaaatttctt agatagaatt tctaataaat agtgtgaaag
     8281 ataaggaggt gtcacaatga ctaactctgt attttcgaca atgcaagata ttgagaatgt
     8341 tgcaaccgat attataaaat catatgataa tgagatttat acgtataaag ctgtctccca
     8401 agaagaattg gaaaaactag aaaaaagtta tgatgaaaaa agtcacgaag aattaatttc
     8461 aatagaaagc aatttagaaa tgaaacaaca gaacctcatt gatgaggtta ataaaacaat
     8521 caaggaaaat gatgcaaaga ttcagtatat ttcatcaagt aggagaggag aatttgtaga
     8581 aaaaattatt ggtagggtgg tagaaaaata tggctattag tcagatgaaa agaatttctc
     8641 tactatttac taagagtagt cttgatgatg ttttaaaaac tattcaagaa ctagagtcag
     8701 tgcagttccg tgatttaaag gttcaggata actggtcaga agcgctagaa aaagatgagg
     8761 ttgtattttc aactattcaa atttctcaca cttctaattc ccatcatggg gttattgaag
     8821 gaaatgatgc cttgaattat ttgatgaata aacaacaaca tttggaagca actgtagaga
     8881 aattacaaga atacctaccg aaagaaaaca cgtttaagtt attacagcaa cctccgataa
     8941 caacctctta tgaagaatta gaaaaatttg gtaaagctaa tgttgctgag ggtgttctta
     9001 aaaaagtgaa tcatcaaata aacagagttc atgaattaga aagaaacatt caaagtaata
     9061 atgaggaaat agagagatta ataaagtggg aaaaattaga aattattcct gcgcatttag
     9121 aacaattttc tttctgtaga ggaaaagtcg gaacaattcc aaggactgta gataatcgct
     9181 tatacaataa ttttttagaa aacaatattg aagttcaaga aatattttct aatgatagag
     9241 agtacggtgt tgttgttttc tatcagtcca gttgctctat tgattttgat gaatacttat
     9301 ttgaaccatt tgattattct agaaaggaat taccgaagca gcgagtagta gatttggatc
     9361 aagaaaacat gcagttaata actgaaaaag agaatattat cgcatcgttg caagattcaa
     9421 agaaaaattt gatagattta caatggcaaa ttgactatat tttatctatc tatgctcgtc
     9481 aaatctctaa gaataacctt ttgagcactc cgcatctagt tgcattagaa ggatggatag
     9541 aagaaactcg tattttatat tttataaaag ttatggatga gcgttttgga cattctattt
     9601 atatttatga atcggaaaca ttgtcggata atcaagatga aatacctatc aaattaacga
     9661 atcactcttt aattgaacca tttgaattat tgacagaaat gtatgctctg cccaaatatt
     9721 atgagaaaga tcctacacct gtattagcac cattttactt tacatttttt ggaatgatgg
     9781 ttgctgattt aggctatggt ttactattgt ttttaggaat aatgttagca ttaaaaattt
     9841 ttcatctacc ttcagtaact aagaaatttt taaaattctt taatatatta ggggtagccg
     9901 ttgcaatttg gggtggaatc tatggctcat tttttggata tgagttgcca tttcatctga
     9961 taactacaac ctcggatgtc atgactatat tagtagtgtc agttgtgttt ggatttatta
    10021 cagtatttgt aggtttgtta gcttcaggac tacaaaaagt aagaatgaag aaatatgcag
    10081 aagcatataa ttcaggattt gcgtggtgtg ttattctgct tggcttgtta tttattgctg
    10141 tcggaatgtt gatgcctgat attagaccgt tatttgtatt agggaaatgg gtatctattt
    10201 ttaatgctgt ggggattttg attgcttcta ttattcaagc caaaagcttg tcaggtattg
    10261 gctcaggatt gtttaatcta tataacattt catcttatat aggtgattta gttagtttca
    10321 ctcgattgat ggcattagga ttatctggag caagtatagc atcagctttc aatttaattg
    10381 ttggtttgtt tccggggaca ttggctaaac tgacaattgg attagtatta ttcattcttt
    10441 tacatgcgat caatattttt ctatcgttac tatcaggata tgttcatgga gcgcgtctga
    10501 tatttgttga attttttggt aagttttatg agggtggagg aaaaccattt caacctttga
    10561 aggcttctga gaaatatatt aaggttatta caaagaatta atggaggata tatataatgg
    10621 aacatttagc aacttatttt tcaacctatg gaggagcttt cttcgctgca ttgggaattg
    10681 tattggcggt tggattaagc ggtatggggt ctgcttatgg agttggtaag gctgggcaat
    10741 ctgccgcagc tttactgaaa gaacagccag aaaaatttgc ctcagctttg atattgcaac
    10801 tattgcccgg aacacaagga ttatatggtt ttgttattgg aattttgatt tggttgcaat
    10861 taactccaga acttcctttg gaaaaaggcg ttgcttattt ctttgtagct cttccaattg
    10921 ctattgtagg atacttttcg gctaagcatc aaggaaatgt agcagtagcg ggaatgcaaa
    10981 tcttggctaa aagaccaaaa gaattcatga agggagcaat tttggctgcc atggtagaaa
    11041 cctatgcaat tcttgccttt gtcgtatcat tcattttgac ccttcgtgta taagaaatta
    11101 atttgctatt caaaggaggt gtctaaatga gcaatttaga aaacttacga gaatctgtta
    11161 ttgaacaagc tcatgaaaaa gggagtatga aattattgga ttccaaaaag aagattgatg
    11221 atgaatttga aatgcaaaag tcggtcattg taaagaaaaa agaagctgaa catgaacgaa
    11281 agttaaaaga attacaacag aaatatcaaa taatttttca acaattaaaa aataaggaac
    11341 gccaatcaac gttagtatca aaacagaaaa tattaaaaga actttttcaa tctgctttaa
    11401 tagaaatgga atcttggagt gcagataaag aaatggagtt catctatcga attctggaac
    11461 gatattcaca acaagaggtc atagtaactt ttggggagcg gactttagct aaattcaatt
    11521 tggaacaatt agagaaattg aaattctctt ttccaaatca tttatttagt gaacagccta
    11581 tctcaaatga atcaggctta cttatttcaa taggtaaaat tgatgataac tatttgtata
    11641 aaacgttaat tggatcgatt tctaaggaag aaagttcaag tatcgcaaat caaattttta
    11701 tcaattaagg atgaaattgg ttaatccttc ttagaaattt ggagtattcc aataaaatta
    11761 gaaaggtatt ttatggatac taatcttttt tcaaaaataa atacgacgat ttcggtaaaa
    11821 gaaaacgatt ttattacaga agaaaaattt caaaaaatta tacaatccaa agatacggag
    11881 acattggcat ttatcttaga agcaactccc tatcatttat cgattgacgt tttagaaaat
    11941 cctagtcaga cagagatttc gctaatgaca aaattagtca atgattatag atgggcctat
    12001 gctgaaagtc cgtctgataa aattgtgact ttatttgctt tacgatatgt ttatcataat
    12061 atcaaagttt tattaaaatc taaggcggca attaagaaag atttttctaa attattaatt
    12121 ccaataggga tttttgatat agaaagttta aaacatttag tttcttcctt acattcagat
    12181 acacttcctg attttatggt tcatgaagta gaatcaattt ggaatgagta tgaaactttt
    12241 aataatattc gtgtacttga tgtcggagct gatctagcat attttaaaca tctgaaactt
    12301 ttatctaatg agttaggtga ggtgctgtct caggttattg ttgcaatgat tgacttttat
    12361 aatattatta ctgtaaaacg tggtttatct caaaataaga gtcatgggga tattttacaa
    12421 ttactttcag atgaaggaag tatttctgct aaagaattta tatacattgt agaaaatcaa
    12481 gaaatatttg tgtggttcaa taaaataaat ccaagcttag attcaatctt ttcaacttac
    12541 gaattgaaga tgcaggacgc aacaatttca tcttctgagt tagaattttt atgtgattta
    12601 ctattgtata aaactttaga tcaaggaagg tataatgtag atgggccgtt agttcttgct
    12661 agatatttat tgggatgtga gtttgaagta aagaatctta gaatgatcat atcagctctt
    12721 caaaatacaa ttccttttga atcaataaaa gaaaggatac gcccgcatta tggaagctaa
    12781 taagtataaa attggcataa ttggtagcca tgatattatt ttacccttta gcatgattgg
    12841 gtttgatata ttttctgcct atcaagaaca agaagctata aatactctaa gaaaattagc
    12901 tcaatctgat tatggtgtca tttatatcac tgaagacatt gcttcaatga tattagatac
    12961 aattcgccat tatgattctc aagttgtgcc tgctattatt ttattaccga ctcataaaca
    13021 aggtttaaat ttaggattaa aacgtataga ggataatgta gagaaagcag taggacacaa
    13081 tattttataa taatgtacaa aattgtctgt aatattattc tataattttt ggacttagta
    13141 aggagaataa ctttgactca agggaagatt ataaaagtat cgggacctct agttattgca
    13201 tcaggtatgc aggaggctaa tattcaagat atttgccgtg taggtaagct agggttaatc
    13261 ggtgaaatta ttgaaatgag aagagatcag gcatctatcc aagtctatga agaaacatct
    13321 ggtcttggtc cgggagaacc tgttgttaca actggagaac ctctctcggt tgaattaggg
    13381 ccaggattga tttctcaaat gtttgatggc atacaacgcc cattagatcg atttaaattg
    13441 gctactcata atgattttct agttcgtggg gtagaagttc caagtttgga tagagatatt
    13501 aagtggcatt ttgattccac tatagcaatt ggtcaaaaag tgagtacggg tgatattctt
    13561 ggaactgtca aggaaaccga ggtagttaat cataaaatta tggttcctta tggagtatct
    13621 ggagaagtcg tttctattgc atctggcgat tttacaattg atgaagttgt atatgaaata
    13681 aaaaaattgg acggtagttt ctataaagga acgcttatgc aaaaatggcc tgtccgcaag
    13741 gcgcgtcctg tttctaaacg tttaattcca gaagaaccat taatcacagg tcaacgagtt
    13801 attgatgcat tctttccagt aaccaaaggg ggagctgcag cagttcctgg accgtttgga
    13861 gcaggaaaga cagttgtaca acaccaagta gctaaatttg ccaatgttga tattgttatt
    13921 tatgtcggtt gtggagaacg tggaaatgaa atgacggatg tactgaatga gtttcctgag
    13981 ttgattgacc ctaataccgg acaatcaatt atgcaacgga cagttctgat tgctaatact
    14041 tcaaatatgc ctgttgctgc tcgtgaggct tcaatttata caggaattac catggctgag
    14101 tattttcgtg atatgggcta ctctgtcgcc attatggctg attcaacttc acgttgggca
    14161 gaagcgctac gtgaaatgtc aggacgtcta gaagaaatgc ctggtgatga gggttatcct
    14221 gcttatctgg gaagtcgtat cgctgaatat tatgaaagag caggacgttc tcaggttcta
    14281 gggcttccag aacgtgaagg aacgattact gctattggag ctgtatcgcc acctggtgga
    14341 gatatttcag aaccagttac tcaaaacact ttacggattg tgaaagtttt ttgggggctt
    14401 gatgctccgt tggcacagcg acgtcatttt cctgcaatta actggcttac atcttattca
    14461 ctatataaag acagtgtggg cacttatata gatggtaaag agaagacaga ttggaatagt
    14521 aaaataactc gtgcgatgaa ctacttacaa cgggaatcta gtttagagga aattgttcgt
    14581 cttgttggaa ttgattctct gtctgataat gaacgactaa cgatggaaat tgctaaacaa
    14641 attcgagaag attatttgca acagaacgct tttgattcgg tagatacatt cacttcgttt
    14701 gcaaaacaag aagcaatgct aagtaatatt ctcacttttg ctgatcaggc aaatcatgct
    14761 ttagagttgg gttcttactt tacagagatt atggaaggta ccgtggcagt tcgagaccgt
    14821 atggcgagaa gtaaatatgt ttcagaagat agattagatg aaatcaaaat tatatcaaat
    14881 gagattacac atcaaattca tttgatatta gaaacaggag gtctataaat gagtgttata
    14941 aaagaataca gaactgctag tgaagttgtt gggcctctta tgattgttga acaagtaaat
    15001 aatgtgtctt acaatgagtt agttgaaatt caacttcata atggagaaat tcgtcgtgga
    15061 caagttttag agatccacga agataaagca atggttcagc tttttgaagg atctagtgga
    15121 ataaatttag aaaagtctaa aattcgtttt gctggtcatg cattagaatt ggctgtatct
    15181 gaggatatgg ttggtcgtat ttttaatggg atgggaaaac caattgatgg tggaccagat
    15241 ttaattccag agaaatattt agatattgat ggtcaagcta ttaatcctgt atctagagat
    15301 tatccagatg aatttattca gacagggatc tcctctattg atcatttgaa tactcttgta
    15361 cgtggtcaaa aattaccagt attttcaggt tcgggcttac ctcataatga attagctgct
    15421 cagatagcaa gacaagcgac tgttttaaat tctgatgaaa attttgcggt tgtatttgca
    15481 gcaatgggta ttacttttga agaagctgag ttttttatgg aagaactcag aaaaacagga
    15541 gcgatcgatc gttcggtttt atttatgaac ttggcaaatg atcctgcaat tgagcgtatt
    15601 gcaactcccc gcattgcttt aactgcggca gagtatctag cttttgaaaa agatatgcac
    15661 gttctagtta tcatgacgga tatgactaac tattgtgaag cgttacgtga agtctcggca
    15721 gctcgccgtg aagttccagg gagacgaggc tatccgggat atttatatac aaatttatca
    15781 actctatacg aaagggctgg tcgcttagtt ggtaaaaaag gttcggtgac acagattcct
    15841 attttaacaa tgccagaaga tgacataaca catccaattc ctgatttaac tggatacatt
    15901 actgaagggc aaattatttt gtcgcatgag ttgtataatc aaggttatcg tccaccaatc
    15961 aatgttttac cttctctctc tcgattaaaa gataagggat ctggagaagg taaaactcgt
    16021 ggagatcatg ctccaactat gaatcaactg tttgcagcct atgcccaagg gaaaaaggtt
    16081 gaagagttag cagtagtatt aggagaatcg gctttatctg atgtagataa attgtatgtg
    16141 aggtttacaa agcgttttga agaagagtac ataaaccaag gattttataa aaatcgaaat
    16201 atagaagata cgttgaatct tgggtgggaa ttactatcaa ttcttcctag aacagagtta
    16261 aaacgtatca aagatgattt gcttgataaa tacttacctt tggtagaagt ttaatccgga
    16321 aatggagtga ttatctatgg tacgtttgaa tgtaaaacca actcgtatgg aattgaataa
    16381 cttaaaggaa cgtttgacaa cagctgaacg tggacataag ttattaaagg ataaaagaga
    16441 tgaattgatg aggcgattta tttctttgat tcgtgagaat aatcaacttc ggaaagaagt
    16501 ggaaagttat ctaattgata atctaaaatc ctttgcagtt gctaaatcat taaagaattc
    16561 tcaaatggtg gaggaattat tttcaattcc atcgaaagaa attgaattat ttgttgagaa
    16621 agaaaatatc atgagtgtaa cagttcctag aatgcatatg aatattactt ctcaaaatga
    16681 gaacagtgaa tacagctatt tatcttctaa tagtgaaatg gatgatgtat ttgctacaat
    16741 gaatagttta atttataaat tactaagact ggcagaagtt gaaaaaacgt gtcagttaat
    16801 ggctgatgaa atagaaaaaa cacgtagacg tgtaaatggt ttagaatact cgattattcc
    16861 aaacttgtcg gaaactattc attatataga attgaaacta gaggaggcag aaagagccaa
    16921 tttagttcgt attatgaaag tgaagtagat cctttattta gattattaat tagatgaaca
    16981 aatatcagct tggataaggc tttaagcctt tctaagcttt ttttattgac agtatcagga
    17041 tatctttttc aaaattttgg tttgttagat aatgaaaatg tttctactaa tctagattta
    17101 ggattagtaa atcgtaaatg taattatata gaaagtaagc gcgtcataac aaggtatcta
    17161 tcattcatgg agctcctcct gtatactatt agtaaagtaa aactattgga ggatatttta
    17221 atgccacaac ctattgttcc tgtagagatt ccacaatctc gtcgttttga ttctaaaaag
    17281 agaaatgata ttctgctt
//
LOCUS       DBERYS010000040        17055 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.040, whole genome shotgun sequence.
ACCESSION   DBERYS010000040 DBERYS010000000
VERSION     DBERYS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.040"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..211
                     /locus_tag="ACL8LA_001557"
     CDS             <1..211
                     /locus_tag="ACL8LA_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1813298.1"
                     /translation="PRKSSKLKPLTVEDKTYNHALSKERSKVENIFAKVKTFKMISTT
                     YRNHRKRFGLRMNLIAGIINHELGF"
     gene            227..724
                     /locus_tag="ACL8LA_001558"
                     /pseudo
     CDS             227..724
                     /locus_tag="ACL8LA_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            881..1306
                     /gene="lacA"
                     /locus_tag="ACL8LA_001559"
     CDS             881..1306
                     /gene="lacA"
                     /locus_tag="ACL8LA_001559"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGS1813299.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1337..1852
                     /gene="lacB"
                     /locus_tag="ACL8LA_001560"
     CDS             1337..1852
                     /gene="lacB"
                     /locus_tag="ACL8LA_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGS1813300.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1863..2792
                     /locus_tag="ACL8LA_001561"
     CDS             1863..2792
                     /locus_tag="ACL8LA_001561"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGS1813301.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2794..3774
                     /gene="lacD"
                     /locus_tag="ACL8LA_001562"
     CDS             2794..3774
                     /gene="lacD"
                     /locus_tag="ACL8LA_001562"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGS1813302.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            4008..4844
                     /locus_tag="ACL8LA_001563"
     CDS             4008..4844
                     /locus_tag="ACL8LA_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS1813303.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4880..5197
                     /locus_tag="ACL8LA_001564"
     CDS             4880..5197
                     /locus_tag="ACL8LA_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1813304.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     DAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5199..6893
                     /locus_tag="ACL8LA_001565"
     CDS             5199..6893
                     /locus_tag="ACL8LA_001565"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS1813305.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            7108..8514
                     /gene="lacG"
                     /locus_tag="ACL8LA_001566"
     CDS             7108..8514
                     /gene="lacG"
                     /locus_tag="ACL8LA_001566"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS1813306.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8637..8816
                     /locus_tag="ACL8LA_001567"
     CDS             8637..8816
                     /locus_tag="ACL8LA_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGS1813307.1"
                     /translation="MTSEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEW
                     DTISFDSDFMPFQQS"
     gene            complement(9095..9856)
                     /locus_tag="ACL8LA_001568"
     CDS             complement(9095..9856)
                     /locus_tag="ACL8LA_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1813308.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(10144..11106)
                     /gene="nrdF"
                     /locus_tag="ACL8LA_001569"
     CDS             complement(10144..11106)
                     /gene="nrdF"
                     /locus_tag="ACL8LA_001569"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGS1813309.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(11294..13453)
                     /gene="nrdE"
                     /locus_tag="ACL8LA_001570"
     CDS             complement(11294..13453)
                     /gene="nrdE"
                     /locus_tag="ACL8LA_001570"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGS1813310.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13534..13752)
                     /gene="nrdH"
                     /locus_tag="ACL8LA_001571"
     CDS             complement(13534..13752)
                     /gene="nrdH"
                     /locus_tag="ACL8LA_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGS1813311.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            14103..14366
                     /locus_tag="ACL8LA_001572"
     CDS             14103..14366
                     /locus_tag="ACL8LA_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGS1813312.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            14372..16105
                     /gene="ptsP"
                     /locus_tag="ACL8LA_001573"
     CDS             14372..16105
                     /gene="ptsP"
                     /locus_tag="ACL8LA_001573"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGS1813313.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            16360..>17055
                     /gene="phtA"
                     /locus_tag="ACL8LA_001574"
     CDS             16360..>17055
                     /gene="phtA"
                     /locus_tag="ACL8LA_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGS1813314.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFL"
BASE COUNT         5382 a         2998 c         3530 g         5145 t
ORIGIN      
        1 tccacgtaaa tccagcaaac tcaagccgct aacagttgaa gataaaacct ataaccatgc
       61 gctatctaag gagagaagca aggttgagaa catctttgcc aaagtaaaaa cgtttaaaat
      121 gatttcaaca acctatcgaa atcatcgtaa acgcttcgga ttacgaatga atttgattgc
      181 tggcattatc aatcatgaac taggattcta gttttgcagg aagtctaata aagaaactag
      241 caaggtcagg ccttacttta ttggtggaca tccaggattt aattgtcctc tacttgatga
      301 tgaagtctat gaagattatt atctagagtt tgagaaagaa gagacttgct ctgttccacg
      361 tcctttccca gaaacaggta tgttggattt ccaagataga agtccatggc tagagggaca
      421 aaaagaaata gatcttagtt atgatctttt cagcaaagat gcagtgactt tggatgaatt
      481 gcaatctcga acaattgccc ttcgttctct taaacatgat aggggattga aagtgcattt
      541 tgcagaattt ccaaacctca tcatctggtc aactttgaac aagggacctt tcattacctt
      601 tgaaccatgg tctggcttgt caacattcct tgaagaagga gatcatttag aagataagaa
      661 gaatgtttgt ctcttagaag ccaatcaggt tgaagaatta gggtttgaga tagaagtttt
      721 ataaaaaagt aaaaaataaa taacaaaaac aaacacaaac tgttgacttc ttcaaacaat
      781 agtagtatat tatgtttgtg aggactaatt gactgttttt taaagacagc caatacactg
      841 ctggaatcca gcatagaaaa aataaaaagg agtatacaat atgtctattg ttatcggtgc
      901 agatgctgca ggtttgagat tgaaagaagt tgtgaaagac ttcttagaaa aagaaaactt
      961 ccaccttgtg gatgttacag ctgaaggtca agactttgtt gatgtgactc tcgctgttgc
     1021 tgcagaagta aacaaagaag aacaaaacct tggtatcgtg attgatgctt atggagctgg
     1081 tccatttatg gttgcaacta agatcaaagg aatggttgct gcagaagttt ctgatgaacg
     1141 ttcagcttat atgactcgtg gccacaacaa ctcacgtatg atcactatgg gtgcacaact
     1201 tgttggtgat gaattggcta aaaacatcgc taaaggattt gttaatggta aatacgacgg
     1261 tggtcgtcac caaatccggg ttgacatgtt gaacaaaatg ggctaattag attatagtaa
     1321 gaaaggtaag ttaaaaatga gaattgcaat tggatgtgac cacatcgtaa cagatgaaaa
     1381 aatggcggtt tcagaatttt tgaaatcaaa aggatatgaa gtcattgact ttggtacata
     1441 tgaccataca cgtactcact acccaatctt tggtaaaaaa gtaggggaag ctgtaactag
     1501 cggtcaagct gatcttggag tatgtatctg tggtactggt gttggtatca acaacgctgt
     1561 aaataaagtt ccaggtgttc gttctgcctt ggttcgtgat atgacaacag ccctttatgc
     1621 taaagaacaa ttgaacgcta acgttattgg ttttggtggt aaaattactg gtgaattgct
     1681 tatgtgtgat atcatcgaag ctttcatcca tgctgaatac aaaccaactg aagaaaacaa
     1741 aaaattgatt gcgaaaattg aacatgttga aagtcacaat gctcaacaaa cagacgcaaa
     1801 cttcttcact gaattccttg aaaaatggga tcgcggagaa taccacgact aagaggtaac
     1861 ctatgatttt aacagttaca atgaacccat ccattgatat ttcctatccc ttggatgagt
     1921 tgaagattga tactgtcaat cgtgtggtgg atgtaaccaa aacggctggt ggtaagggac
     1981 tcaatgttac ccgagtactt tcagaatttg gcgattctgt tcttgctact ggtttagtgg
     2041 gtggcaaact tggtgagttt ttggttgaac atatcgataa tcaagtaaag aaagatttct
     2101 tctcaattca gggagaaact cgtaactgta tcgctattct ccacggagac aaccaaacag
     2161 aagttcttga aaaaggtcct gaagtattgg aacaggaagg tcaagacttt ttggaacatt
     2221 tcaaaaaact cttggagtca gttgaagtag tggccatctc aggtagtctg ccagctggcc
     2281 ttccagttga ttactatgcg agcttggtag aacttgctaa tcaagctggc aagcctgtag
     2341 tcttggactg ctcaggtgca gcacttcagg ctgttcttga atcaccccat aaaccaacag
     2401 tcatcaaacc aaataatgaa gaattgtctc agcttcttgg aagagaagtt tctgaggatt
     2461 tggatgaatt aaaagaagta cttcaagaac ctttgtttgc agggattgaa tggattatcg
     2521 tttcacttgg tgccaacggt acttttgcca aacatggtga cactttctac aaggtagata
     2581 ttcctagaat tcaggtggta aatcctgttg gatctggaga ctctactgtg gcaggaattt
     2641 cttcaggact tcttcacaaa gaatcggatg cagaattact catcaaggca aatgtccttg
     2701 gtatgctcaa tgctcaagaa aaaatgactg gtcatgtcaa catggccaac tatcaagttc
     2761 tatatgatca attaatagta aaagaggtat aaaatggctt taacagaaca aaaacgtgta
     2821 cgcttagaaa aactttctga tgaaaatggt atcatctcag ctcttgcatt tgaccaacgt
     2881 ggtgctttga aacgcctcat ggctcaacac caaacagaag aaccaactgt ggcccaaatg
     2941 gaagaactta aagtcttggt agcagatgaa ttgactaaat acgcttcatc tatgcttctt
     3001 gaccctgagt atggacttcc agcaactaaa gctcttgatg aaaaagctgg tcttctcctt
     3061 gcttatgaaa aaacaggtta tgacacaaca agcacaaaac gcttgccaga ctgcttggat
     3121 gtctggtctg caaaacgtat taaagaagaa ggtgcagatg cagttaaatt cttgctttac
     3181 tatgatgtag atagctcaga cgaactcaat caagaaaaac aagcctacat cgaacgcatc
     3241 ggttctgagt gtgtggctga agatatccca ttcttccttg aaattcttgc ttacgatgaa
     3301 aaaattgcgg atgcaggttc tgtagaatac gcgaaagtaa aaccacacaa agttatcggc
     3361 gctatgaaag tcttttcaga cccacgcttt aacattgatg tcttgaaagt tgaagttcct
     3421 gttaacatta aatatgttga aggcttcgct gaaggtgaag tagtttatac acgtgaagaa
     3481 gcagcagcct tcttcaaagc gcaagatgaa gcaacgaact tgccatacat ctacttgagt
     3541 gctggtgtat cagctaaact cttccaagat actcttgtat ttgctcatga atcaggtgca
     3601 aactttaacg gagttctttg tggccgtgca acatgggctg gatcagttga agcatacatc
     3661 aaagatggtg aaacagcagc tcgcgaatgg cttcgcacaa ctggatttga aaacattgat
     3721 gagctcaaca aagttcttca aacaacagca acttcatgga aagaacgtgt gtaagaaagt
     3781 cctcctagtt taggaacatg aatctaaaaa aatttaaaaa aagttgtatg taaaggttta
     3841 caaaataact tacttgtgct atacttaaat cacaagttaa tacaaggtga gtgttactaa
     3901 gtaatattag gcatgatcac aggtgaatta gaaatcagct gattttctag ttcatttgtg
     3961 gtcatttttt gtactcatat acctttaaga tataaaagga ggttgacatg tatcgaattc
     4021 taaatccaat gaatcacaat gtctcgcttg tcagaaatga taagggagaa gaggtgattg
     4081 taattggtaa ggggattgca ttcggaaaga agaaggggga tttgattgct gaaaatcatg
     4141 ttgagaaaat ctttcggatg aagaccgaag agtccagaga aaactttatg gctcttctca
     4201 aagatgttcc gcttgatttt atcacagtga cctatgaaat cattgataag ctatcaaaga
     4261 aatatcatta tccgattcaa gagtatctct atgtaacctt gacagatcat atttactgtt
     4321 cttatcaagc tctaactcaa ggaaggtaca aggatagtaa tctgccagat atttctgcta
     4381 agtatcctgt cgcttttcaa atcgcaaatg aagcttttga aatttactgt cagaagctag
     4441 cagatcattt tcctgaggac gaaattattc ggattgctta tcatttcatt aatgctgaag
     4501 gtgaaaatga agtggaactt gtggagtcga ttgataagag gaaagaaatt ctcaggaatg
     4561 ttgaagaagt tttaacggac tatgcaattc aacgaactaa aaagaataac catttctatg
     4621 atcgctttat gatccatttg aattatttct tggattattt agacagatct agagatgata
     4681 accaatcact tctggatatg gaagatcata ttaaacaatc ctatccaaaa gccttcgaga
     4741 ttggttccaa gatctatgat gtgattacgc aacatacggg tcttgatttg tataaaagtg
     4801 aacgagttta tctagttcta catatccaac gtttattgtc ataaaaattt atttaaaact
     4861 atataaggag aattctatca tgaatagaga agaagtaaca ttgttaggtt ttgaaatcgt
     4921 agcctatgct ggcgatgctc gttcaaaact attggaagcc ttgaaggctg ctgaagctgg
     4981 tgattttgaa aaagcggacg ctctggtaga ggacgctggt agctgtattg cagaggctca
     5041 ccacgcgcaa acaagtctat tgactaagga agcttctggt gaggacttag cgtatagtgt
     5101 gaccatgatg catggtcaag atcacttgat gacaactatc ttgttaaaag acctaatgca
     5161 ccatttaatt gaactttaca agagaggagt taaataaaat ggataagtta ataattttta
     5221 ttgagaaagg aaagcctttc tttgagaaat tatcgagaaa tatatactta agagcgataa
     5281 aagatggttt tatttccagt atgccagcag tattattctc aagtattttt atactaatag
     5341 ctgctgtgcc caatattttt gggtttaaat ggtctgatga acagttagct tttattttga
     5401 aaccttacaa ctactcaatg gggattttag ctctcttagt agctgggaca actgcaaaat
     5461 cgttaacaga ttcagttaat acacgtagta tggagaaaac caaccaaatc aactacatgt
     5521 caactttttt agcagctgtt gttggattgt taatactggc agctgatcca attgaaggtg
     5581 gatttgctaa tggtctttta ggtactagag gattacttac tgcatttttg gcagctttta
     5641 ttacagtaaa tatctataaa gtatgtatta aaaataatgt aaccattaga cttcctgaag
     5701 aggttccacc aaatattgca caagttttta aagatgttat tccgtttgcc ttatctgttc
     5761 tttcaatata tggattggat ttaattgtta gaaatatttt tggtacaaat gtagcagaat
     5821 ctgttggtaa aatattagcc cctctatttt cagctactga tggttatatc ggtttagcta
     5881 ttgtatttgg tgcttatgct ttcttttggt ttgttggaat tcatgggcca tctgttgtag
     5941 aacctttaat tgtagctatc agttatgcaa atatagaagc taatgttcag cttgtgcaag
     6001 ctggtatgca cgcagataaa attttaaatc cagttactca aacttttgtt gtaacaatgg
     6061 gtggtactgg tgcaacacta gtagttccat ttatgtttat gtggttatgt aaatcaaaaa
     6121 gaaatagaat tgtcggacgt gcgtctgtag ttcctacttt ctttggcgta aatgaaccaa
     6181 tcctatttgg tgctccaatt gttttaaatc ctatcttctt tattccgttt gttactgctc
     6241 ctattatcaa tgtttggatt atgaaatttt ttgtagatgt tctacaaatg aatagtttca
     6301 gtattatttt accttggaca acacctgctc caattggtat tgttatggga acggcgctgg
     6361 ctccattgtc attcgtcttg gctataactt taatcattat cgacacttta atctattatc
     6421 catttgttaa agtttatgac catcaaattt tagaagaaga acggaaagga aactcttcat
     6481 ctgaattgaa agaaaaagtt gctgcaaact tcaacactgc aaaagcggat gctattcttg
     6541 aaaaagcagg tgtcgatgca gcacaaaata ccatcactga agaaacaaat gtcctcgttc
     6601 tctgtgctgg tggaggtaca agtggactcc ttgcaaatgc tttgaataag gcagcagcag
     6661 aatacaatgt ccctgtgaaa gcagcagcag gcggctatgg tgctcaccgt gaaatgttac
     6721 cagagtttga tttggttatc cttgctcctc aagttgcttc aaactttgaa gatatgaagg
     6781 cagaaacaga taagctcggt attaaactag cgaaaacaga aggcgctcaa tacatcaaat
     6841 taactcgtga tggaaaaggt gccctagcat tcgtacaaga acaattcgat taaggctagg
     6901 aactgtgaaa cagtctccta tcgttacgga aatcgctatg gcgaatttcc taatcgcctt
     6961 attaattcgt cggtaaaaag atatcgtttt tacctcctca tgtcacaatt cggtgacttg
     7021 gtacaagaag tgagatggag aaggatggct cactgactcc tctcctctca cttttacttt
     7081 atttaaatca agaaataggt gaaaaaaatg acaaaaacac ttccaaaaga ctttattttt
     7141 ggtggcgcaa cagctgccta tcaagcagaa ggtgctacac atactgatgg taaaggacca
     7201 gtagcgtggg ataaatatct tgaagataac tactggtaca ctgcagaacc agccagtgat
     7261 ttttacaatc gatatccagt tgatctcaag ctagcagaag agtatggtgt caatggcatt
     7321 cgaatttcta ttgcctggtc acgtattttc ccgactggtt atggtcaagt aaatgctaaa
     7381 ggtgttgagt tttatcataa tttatttgca gagtgtcaca aacgtcatgt tgagcccttt
     7441 gtaactcttc atcactttga cacgccagaa gctctccact caaatggaga cttcttaaac
     7501 cgtgaaaata tcgaacattt tgtagactac gctgccttct gttttgaaga atttccagaa
     7561 gtaaactatt ggacaacctt caatgaaatt ggaccaatcg gtgatggtca atatttggtt
     7621 gggaaattcc ctccaggtat ccagtacgac cttgccaaag tctttcaatc acaccacaat
     7681 atgatggtgt ctcatgcatg cgcggtaaaa ttgtacaaag agaaaggcta taaaggggaa
     7741 attggtgttg ttcacgccct gcctactaaa tatcctctag atcctgaaaa tccagcagat
     7801 gttcgtgcag cagagttgga agatatcatc cacaataaat tcatcttaga cgcaacttat
     7861 ctaggtcgct attcagctga aaccatggaa ggtgtcaacc atatcttatc agtcaatggt
     7921 ggtagtttgg atcttcgtga agaagatttt acagcattag aagctgcaaa agacttgaat
     7981 gatttcctag gaatcaacta ctatatgagt gactggatgg aagcctttga tggagaaact
     8041 gaaattatcc ataatggtaa aggtgaaaaa ggaagctcta agtatcaaat caaaggtatt
     8101 ggtcgtcgtg tagctcctga ctatgtatca cgcacggatt gggattggat tatctaccct
     8161 caaggtttgt atgaccaaat catgcgtgtg aagaaagatt atcctaacta caagaagatt
     8221 tacatcactg aaaatggtct cggctataaa gatgagttcg ttgataacac tgtttacgat
     8281 gatggtcgta ttgattacgt gaaacaacac ttggagatct tgtctgatgc gattgcagat
     8341 ggagctaatg taaaaggtta cttcatttgg tcattaatgg atgtcttctc atggtcaaac
     8401 ggttatgaga aacgttatgg tctcttctac gtagattttg aaactcaaga acgttatcct
     8461 aagaaatcag ctcactggta caagaaagta gcggaaactc agattataga ctagtagaat
     8521 tagtcattag atatagaatt ttagtgagtc aaaaagatgt tcaaagattt tatccaatct
     8581 atttatgaaa aaaagtttat attataaatt tcgaaaaatg ctctcaaata ccgtgtttga
     8641 cgagtgaaga attgaaaagt cttggaaaat ggtatgtctc gactggtaaa gaatggattt
     8701 gtcattcaga tgatgagctg gaagaattta aaaatctatt tttaaatttt atcaatcctg
     8761 aagaatggga tactatctcc tttgattcag attttatgcc gtttcaacaa tcgtaaccaa
     8821 tttctcaaaa aagttaaatc ttatatttag tactctgtaa aactcttatc taatcacgtt
     8881 gcttatactc aatgaaaatc aaagagcaac tttaaactag gaagcgagtc gcagatttct
     8941 caatgcatag ctttgaggaa ttgggcaaaa agtctttgat atagaaaaaa gcatagtatc
     9001 aggtgttgaa acacctgata ctatgctttt tattgtggga agatttactt tttttcttct
     9061 gaaattgagt tgttacccag gctctttcag tttattaagg cttgatgact ttaatgtgtt
     9121 tagatagctt aaaaaggatt gaatcactta gtttagaatc tgaaacaata gtatcaagat
     9181 ttgatatatt ataaaaagta taaaaatcaa acttattgaa cttactatga tctgcgagta
     9241 aatatttttt attagaatta tttaaagcga tgcgttgagc ctctccctct tcctcgctaa
     9301 aagtagctag agctccgttt tgaataccat tacagctaac gaaagcttta gaaaattgga
     9361 gattagagag attttgtagg gtcaatgtgc caacaaaagc acctgtaata tcgcgataat
     9421 ttccacctat caaaatcaaa tctgttaatt ttcgttcgct taaaatcaga aaaacaggta
     9481 gactgttggt tacgacgcgg atattgtcaa taggcaactc acgcgcaaaa aactctaatg
     9541 ttgttcctgg tccaatgaaa atagtttctc tttcttctac tagactgcct gcaaaatggg
     9601 ctatttcttg tttttctgcc gtttggaggg cttgtttttc aatatttgat cgctcattag
     9661 tcaaaaggga gttggttcga agtttttcag ctccaccatg cacacgaatc agcaaatctt
     9721 tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc tgaaacggct atttcgtcca
     9781 taatctgttt aactgttatg gttcctttac tatttactat ctctaaaatt ttggttaatt
     9841 tttcttgttt gagcatatta tcacctcgtt tcctactact atcttaccat aaacaaactc
     9901 atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa aaatatcgaa gtttgttttc
     9961 aaaactttcg atatttttgt tgggttatta ttttgttgtt tctagtttac tttttgatgg
    10021 ttaagagtgg tggagaatta tactcaatga aaatcaaaga gcaaactagg aagctagccg
    10081 caggctgtac ttgagtacgg caaggcgaag ctgacgtggt ttgaatttga ttttcgaaga
    10141 gtattagtcc aaaccgtagt tgtagtcatc atcttgcatg gcttcaactt cgccaagaag
    10201 gtaaccattt ccgacttgag agaagaagtc atggttggaa gttcctgttg aaataccgtt
    10261 cataacgatt gggttgacat cttcagctga atctgggaaa agtggatctt gtcccatgtt
    10321 catgagagct ttattggcat tgtagcgaag gaaggtttta acctcttcag tccaaccaac
    10381 accgtcataa agactctctg tgtagccttc ttcattttca taaagagtat agagtaggtc
    10441 gtacatccat tctttgagtt tttcttgctc ttcttcaggt aattcattga aaccaagttg
    10501 gaatttgtaa ccaatgtagg ttccgtgaac agactcgtca cgaataatca atttaatgat
    10561 ttctgcaacg ttggcaagtt tgttgttacc gagatagtag aggggagtga agaaaccaga
    10621 gtagaagagg aaggtttcga ggaagacgct ggcaactttc ttttcaagtg ggctgccgtt
    10681 taggtagatt tcgttgacaa tctcagcctt cttttgtagg taaggattgg tattggtcca
    10741 ttcgaaaatt tcttcaatct cagccttagt attcaaggta gaaaagattg atgagtaaga
    10801 tttagcgtgg acagattcca taaattggat gttattgaaa acagcttcct catgtggtgt
    10861 acggatgtct gcgcgaaggg cttgaacccc agtttcagat tgcatagtgt caagaagggt
    10921 taaaccacca aaaacttttc cgaccaagtc tttctctttg ttagatagct ttctccagtc
    10981 atccaagtcg tttgataagg gaatacgtgt atcgagccaa aattgctccg tcagtttttc
    11041 ccaagttgat ttgtcgatga catcttcgat ggcattccag ttaatggctt tgtagtaagt
    11101 ttccatttaa aatctctttc tgtgtttagt attgcgaact cacaattatt tctactttac
    11161 cataattcta taggagtatc gcacaaaaag tcggaagccc gacttttaaa atgttacata
    11221 aattatgtta tgacatagta gatttgattt tatcagtgct gcttagggaa aaatagtgtt
    11281 tctatgctag aaactaaatc acacagcttt cacattggtt ggcgccgact tctccaccgt
    11341 catctgtaaa ggtacggacg tagtagatag acttgattcc cttgttaaag gcatagttac
    11401 gaaggatgga caagtcacgt gtcgtttgtt tattttccct cttccattcg taaaggcctt
    11461 ttggaatgtc actacgcatg aagagggtga gtgaaagtcc ttgatccacg tgttcagtcg
    11521 cagcagcgta aacatcgatg actttacgca tatccatatc gtaggcagaa gtgtagtaag
    11581 gaatggtttc tgtagacaag ccagcagcag ggtagtagat tttaccaatt ttcttttcct
    11641 gacgttcttc aatacgttgc gtaatcgggt ggatagaagc agaaacatcg ttgatgtagc
    11701 tgatagaacc atttggcgct acagcaaggc gattttggtg gtaaagacca tcctcttgaa
    11761 ccttgtcgcg aagttcagtc caatcagcaa caccagggat aaaaacattt ttgaagagtt
    11821 ctttaacacg gtctgatgtt ggaacaaatt caccagttac atacttgtca aagtaacttc
    11881 cgttagcata gtctgatttt tcaaagttgt ggaaggtaat accacgttca cgcgcaatat
    11941 tgtttgactc taccaaggtc cagtagttca taagcataaa gtagatgctt gtaaattcaa
    12001 cagactcagg tgaaccatat tcaatgagtt gttgggcaag gtagctgtgc agtcccatgg
    12061 caccgagacc aaaggtgtgg gcttggctat ttccatggtc aatcgttggt acagctacga
    12121 tatgtgaact atctgtaacg aaagtaaggg cacgaaccat agcacggata gaacgaccaa
    12181 aatcaggtga agtcatcatg ttaaccacgt tggttgaacc caggttacat gaaacatctg
    12241 ttcccatttg aaggaattct tgagcatcgt tgatcaagct tggttcttga acttgaagaa
    12301 tctcagaaca caagttactc atgataatct ttccatcgac aggatttgca cggttagccg
    12361 tatctatgtt gactacataa ggatagccag actcttgttg caatttagag atttcagttt
    12421 ccaaatcccg cgccttgatt tttgtcttgc gaatatttgg atttgcgacc aattcatcgt
    12481 atttttcagt aatgtcgatg taattgaatg gcacaccgta ttctttttct acagagtaag
    12541 ggctgaagag gtacatttct tcatttttac gagccaattc gtagaattta tcaggtacta
    12601 caacaccaag tgatagagtc ttgacacgta ctttttcatc agcgttttct ttcttagttg
    12661 aaaggaaagc gatgatatct gggtgaaaga cgttgaggta gacaacaccg gcaccttgac
    12721 gttgccccaa ttggttggag taagagaagc tgtcttcaaa aagcttcata acaggaacga
    12781 cacctgaagc agctccttca tagcctttga taggtgcacc agcttcacga aggttgctga
    12841 gggtaattcc cacaccacca ccaatacgtg aaagttgaag agctgagttg atagaacgtc
    12901 cgatagagtt catatcatcc gtcacttgga ttaggaaaca agataccaac tccccacgac
    12961 gagcacgtcc agcattcaag aaggaaggag tagcaggttg gtagcgttgg tggatgattt
    13021 cattggcaat atcgattgca acagcttcat tcccatcagc gaaataaagg gcgttgaaga
    13081 agacacggtc ttccatattt tcaagatagt attcaccgtc attagtcttt aaggcatatt
    13141 gattgtaaaa tttataagct gccatgaatg acttgaattg gaagttttgg tctttgataa
    13201 attgagccaa ttcttccaag aactctggac ggtatttctt gataaaggct gtttcgatgt
    13261 agttgtgttc aatgaggtaa ttgattttgt ctttgattga atcaaaaacc atagtgtttg
    13321 gaactacatt ttctttaaag aaagcatcca aggcttcctt atctttatga agcatgattt
    13381 gtccattaac aggacggtta atttcgttat taagacggaa gtaagtcacg tcttcaagat
    13441 gttttaatcc cataaaattt cccttatcta attacaaaag aaaggcttct aagttagccc
    13501 taaaagcagt ttcttctgga tgatgtacta agattatgct aattgtttca gtttttctgg
    13561 ttggaaacct gaaaagactt cagttggtgt ttggataaca ggagctgcgc taaaaccgag
    13621 ctctttaact tgatcgacgt actcaggttg ctcatcaaga ttgatttcac gataagagac
    13681 attattactg tccaagaaac gcttggtcat tttacattgg acacaattgt ttttagaata
    13741 aacggttacc attgtgtaac tcctcttcaa aatttaatac tatcttagta tatcagaaaa
    13801 taaaattttg tcaatgattt aaactaaata tagtggtgtt cttttgttgt ttttatatat
    13861 aaacacaata tatagtgttt gtattgtgaa atagcaatga ttatcctata aattgttttt
    13921 gaagaaacta tatcctgtgt tttgttatct aggatagaag attttcagca agttatctga
    13981 aaggaaattg aaggaaaatc cagaaaatgc ttgaaaaaaa tcctagaaga tggtataata
    14041 ctaaattgta agggttatca catataactc aaaaaaagaa agaacaaaag gagagtcaaa
    14101 ctatggcttc taaagatttc cacgtagtgg cagaaacagg tattcacgca cgtccagcaa
    14161 cattgttggt acaaactgct agcaaatttg cttcagatat cactcttgag tacaaaggta
    14221 aatcagttaa ccttaaatca attatgggtg ttatgagtct tggtgttggt caaggtgctg
    14281 acgtaactat ctcagctgaa ggtgcagatg cagatgacgc tatcgctgca atctcagaaa
    14341 caatggaaaa agaaggattg gcataaggga aatgacagaa atgcttaaag gaatcgcagc
    14401 atctgacggt gttgcagttg caaaagcata tctactcgtt cagccggatt tgtcatttga
    14461 gactattaca gtcgaagata caaacgcaga agaagctcgc cttgatgccg ctctacaggc
    14521 atcacaagac gagctttctg ttattcgcga gaaagcagta ggtacgctcg gtgaagaagc
    14581 agctcaagtt tttgatgctc acttaatggt tcttgctgac ccagaaatga tcagccaaat
    14641 caaggaaact atccgtgcga agaaagtgaa tgcagaagca ggtctgaaag aagttacaga
    14701 tatgtttatc actatctttg aaggcatgga agacaaccca tacatgcaag aacgcgcagc
    14761 ggatatccgc gacgtgacaa aacgtgtatt ggcaaacctt cttggtaaaa aattgccaaa
    14821 cccagcttct atcaatgaag aagtgattgt gattgcgcat gacttgactc cttcagatac
    14881 agctcaattg gacaaaaact ttgtaaaagc ttttgtaacc aacattggtg gacgtacaag
    14941 ccactcagct atcatggcac gtacacttga aattgctgct gtattaggta caaacaacat
    15001 cactgaaatc gttaaagacg gtgacatcct tgctgttaac gggatcactg gagaagtgat
    15061 tatcaacccg acagatgaac aagcggcaga atttaaagca gctggtgaag cctatgcgaa
    15121 acaaaaagct gaatgggcac ttttgaaaga tgctcaaaca gtgactgctg acggtaaaca
    15181 cttcgagttg gctgccaata ttggtactcc aaaagacgtt gaaggtgtta acaacaacgg
    15241 tgcagaagct gttggacttt accgtacaga gttcttgtac atggattctc aagacttccc
    15301 aactgaagat gagcagtatg aagcatacaa ggctgttctt gaaggaatga acggtaaacc
    15361 tgttgtcgtt cgtacaatgg atatcggtgg agataaggaa cttccttact tcgatatgcc
    15421 tcacgaaatg aacccattcc ttggattccg tgctcttcgt atctctatct ctgagactgg
    15481 agatgctatg ttccgcacac aaatccgtgc tcttcttcgt gcgtctgttc acggtcaatt
    15541 gcgtatcatg ttcccaatgg ttgcgctctt gaaagaattc cgtgcagcga aagcagtctt
    15601 tgatgaagaa aaagcaaacc ttcttgctga aggtgttgca gttgcggata acatccaagt
    15661 tggtatcatg atcgagattc ctgcagcggc tatgcttgca gaccaatttg ctaaagaagt
    15721 tgacttcttc tcaattggta caaacgactt gatccaatat acaatggcag cagaccgtat
    15781 gaacgaacaa gtttcatacc tttaccaacc atacaaccca tcaatcctac gcttgattaa
    15841 caatgtgatc aaagcagctc acgctgaagg taaatgggct ggtatgtgtg gtgagatggc
    15901 tggtgaccaa caagctgttc cacttcttgt cggaatgggc ttggatgagt tctctatgtc
    15961 agcaacatct gtacttcgta cacgcagctt gatgaagaaa ctcgacacag ctaagatgga
    16021 agagtacgca aaccgtgccc ttacagaatg ctcaacaatg gaagaagttc ttgaacttca
    16081 aaaagaatac gttaattttg attaatcgaa aagtccctgc aactcagtta cagggacttt
    16141 ttttgatatt ttaaaaagaa ttttcaagaa aatctttctt ataaaaagtc caaccttgaa
    16201 aaagtagtgg tcagaacaaa aaatacttaa atggttcata aaattcttga caagttggag
    16261 atttaggagt aaactattaa ccagttaagt aatagagagg agtttctgca atttagaaat
    16321 gaattgcaac tagaaatatc aaatagaaag agagtttcga tgaaaattaa taagaaatac
    16381 cttgttggtt ctgcggcagc tttgatttta agtgtttgtt cttacgagtt gggactgtat
    16441 caagctagaa cggttaagga aaataatcgt gtttcctata tagatggaaa acaagcgacg
    16501 caaaaaacgg agaatttgac tcctgatgag gttagcaagc gtgaaggaat caatgctgag
    16561 caaatcgtca tcaagataac agaccaaggc tatgtcactt cacatggcga ccactatcat
    16621 tattacaatg gtaaggttcc ttatgatgcg attttcagtg aagaattact catgaaagat
    16681 ccaaactata agctaaaaga tgaggatatt gttaatgagg tcaagggtgg atatgttatc
    16741 aaggtagatg gaaaatacta tgtttacctt aaggatgctg cccacgcgga taacgtccgt
    16801 acaaaagagg aaatcaatcg acaaaaacaa gaacatagtc aacatcgtga aggtgggact
    16861 ccaagaaacg atggtgctgt tgccttggca cgttcgcaag gacgctatac tacagatgat
    16921 ggttatatct ttaatgcttc tgatatcata gaggatactg gtgatgctta tatcgttcct
    16981 catggagatc attaccatta cattcctaag aatgagttat cagctagcga gttggctgct
    17041 gcagaagcct tccta
//
LOCUS       DBERYS010000041        15637 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.041, whole genome shotgun sequence.
ACCESSION   DBERYS010000041 DBERYS010000000
VERSION     DBERYS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.041"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            262..1821
                     /locus_tag="ACL8LA_001575"
     CDS             262..1821
                     /locus_tag="ACL8LA_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813315.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(1970..2869)
                     /gene="htpX"
                     /locus_tag="ACL8LA_001576"
     CDS             complement(1970..2869)
                     /gene="htpX"
                     /locus_tag="ACL8LA_001576"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGS1813316.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(2871..3431)
                     /locus_tag="ACL8LA_001577"
     CDS             complement(2871..3431)
                     /locus_tag="ACL8LA_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGS1813317.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            3525..4238
                     /gene="rsmG"
                     /locus_tag="ACL8LA_001578"
     CDS             3525..4238
                     /gene="rsmG"
                     /locus_tag="ACL8LA_001578"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGS1813318.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            4541..5824
                     /locus_tag="ACL8LA_001579"
     CDS             4541..5824
                     /locus_tag="ACL8LA_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGS1813319.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6019..7590)
                     /gene="ffh"
                     /locus_tag="ACL8LA_001580"
     CDS             complement(6019..7590)
                     /gene="ffh"
                     /locus_tag="ACL8LA_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGS1813320.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(7602..7934)
                     /locus_tag="ACL8LA_001581"
     CDS             complement(7602..7934)
                     /locus_tag="ACL8LA_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGS1813321.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(8025..8402)
                     /locus_tag="ACL8LA_001582"
     CDS             complement(8025..8402)
                     /locus_tag="ACL8LA_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGS1813322.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            8474..9778
                     /locus_tag="ACL8LA_001583"
     CDS             8474..9778
                     /locus_tag="ACL8LA_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS1813323.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            9791..10597
                     /gene="yidA"
                     /locus_tag="ACL8LA_001584"
     CDS             9791..10597
                     /gene="yidA"
                     /locus_tag="ACL8LA_001584"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGS1813324.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            10640..11638
                     /locus_tag="ACL8LA_001585"
     CDS             10640..11638
                     /locus_tag="ACL8LA_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HGS1813325.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNDLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(11804..11875)
                     /locus_tag="ACL8LA_001586"
     tRNA            complement(11804..11875)
                     /locus_tag="ACL8LA_001586"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11840..11842),aa:Arg,seq:cct)
     gene            complement(11917..12264)
                     /gene="rplS"
                     /locus_tag="ACL8LA_001587"
     CDS             complement(11917..12264)
                     /gene="rplS"
                     /locus_tag="ACL8LA_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGS1813326.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(12383..12712)
                     /gene="crcB"
                     /locus_tag="ACL8LA_001588"
     CDS             complement(12383..12712)
                     /gene="crcB"
                     /locus_tag="ACL8LA_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS1813327.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(12706..13080)
                     /gene="crcB"
                     /locus_tag="ACL8LA_001589"
     CDS             complement(12706..13080)
                     /gene="crcB"
                     /locus_tag="ACL8LA_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS1813328.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(13077..13343)
                     /locus_tag="ACL8LA_001590"
     CDS             complement(13077..13343)
                     /locus_tag="ACL8LA_001590"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGS1813329.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(13459..13902)
                     /locus_tag="ACL8LA_001591"
     CDS             complement(13459..13902)
                     /locus_tag="ACL8LA_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGS1813330.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            14006..14941
                     /locus_tag="ACL8LA_001592"
     CDS             14006..14941
                     /locus_tag="ACL8LA_001592"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGS1813331.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            15037..15279
                     /locus_tag="ACL8LA_001593"
     CDS             15037..15279
                     /locus_tag="ACL8LA_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGS1813332.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(15336..15554)
                     /locus_tag="ACL8LA_001594"
     CDS             complement(15336..15554)
                     /locus_tag="ACL8LA_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813333.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
BASE COUNT         4801 a         3429 c         2834 g         4573 t
ORIGIN      
        1 atggacctgt ttttggattt tagagcatag aatttccagt tttctgttga caaaatttcc
       61 ttaaaggtat aatataaaga tactaatact cggaggtaag ggagacatga acaactaagt
      121 ctatcaaata aagaaccttt atttagtaga tcttgttttt gtctcttttt gtgtgctctt
      181 ttatgctctt tttctggcat gttaatagag tttttttgac atagactttg ggctctacta
      241 ggtaaagtag agctttttgt tatgcactat cgacattcta gaaagggcaa tagtatgata
      301 aaaatcaatc acctgaccat cacacaaaac aaagatttac gagaccttgt ctctgaccta
      361 agcatgacca tacaagacgg ggaaaaggta gctattattg gcgaagaagg aaatggtaaa
      421 tcaaccttac ttaaaacttt aatgggggaa gatttgtctg atttcactat caagggagac
      481 atccaatctg actatcagtc actggcctac attcctcaaa aacttcccga ggagctgaaa
      541 aagaaaactt tacacgacta cttcttttta gattctattg atttagacta cagtatcctc
      601 tatcgtttgg cggaggaatt gcattttgat agcaatcgtt tcgcaagtga ccaagagatt
      661 ggcaatctat cagggggaga agctttgaaa attcagctta tccatgagtt agccaaaccc
      721 tttgagattc tatttttaga tgaaccttca aatgacctag accttgagac agttgattgg
      781 ctaaaaggcc agattcaaaa gaccaggcaa accgttattt tcatttccca tgatgaagac
      841 tttctttctg aaacggcaga cactattgtt cacttgcgac tggtcaaaca ccgtaaagaa
      901 gcggaaacgc tagtagagca tttagactat gatagctata gtgagcagag aaaggctaat
      961 tttgccaaac aaagtcagca agctgctaac aaccaaagag cctacgataa aaccatggaa
     1021 aaacatcgga gagttaagca aaatgtagaa actgcgcttc gagctaccaa agatagtact
     1081 gccggtcgcc tattggctaa aaagatgaaa actgtcctct cacaagaaaa acgctacgaa
     1141 aaggcagctc agtccatgac tcaaaaacca cttgaagagg aacaaatcca acttttcttt
     1201 tcagacatcc aaccattacc agcttctaaa gtcttagtcc aactggaaaa agaaaatttg
     1261 tccattgacg accgagtttt ggttcaaaaa ctacaactaa ctgtccgtgg ccaagaaaaa
     1321 atcggtatta tcgggccaaa tggtgttggg aaatcaactc tgttagccaa gttacagaga
     1381 cttctgaatg ataaaagaga gatttcactt ggttttatgc cacaagatta ccacaaaaaa
     1441 ctgcaattgg atttatcccc aatagcctat ctcagtaaaa ctggggaaaa agaggaacta
     1501 cagaaaatcc aatctcacct agctagtctc aatttcagtt atccagaaat gcagcatcaa
     1561 attcgctcct tatctggcgg acaacaggga aaactcctgc ttttggattt agtcctgcgc
     1621 aaaccaaact ttctcctgct ggatgaaccc acacgaaact tttctcccac ttctcaaccc
     1681 caaatcagaa aactctttgc cacttatcct ggcggtctca tcactgtttc gcatgaccgt
     1741 cgtttcttaa aagaagtctg ctcgatcatc tatcgcatga cagaacacgg tttgaagcta
     1801 gttaatttag aagatttata aatttgcaac atagcagata ggtggggcaa aaacacaatt
     1861 ttaggagaaa aagaagtaaa tcttcccata gtaaaacgca taatatcaag ttttcacacc
     1921 tgatattatg cgtttttcag attttaaaga cttttttcca gccttcattt tacatatgtt
     1981 ttaaacgttc aatccgttct gagataggtg ggtgggtata aaagagtttt tggaaccccc
     2041 cacctttctt gggatcattg atataaaggg cactgctagc atcatcgacg tggcgactca
     2101 taggtttgct attgtccaac ttatctaggg cattaatcat tccctgggga ttgcgagtca
     2161 gctcgacact agatgcatct gccagaaatt ccctctgacg agaaatagcg agctgaacca
     2221 aggttgcagc gagaggtgcc agtacaatag ctagtaggga aaccactagc ataatgattt
     2281 caagaccatt tccatctcgg tcatcatcac ttcgtctgcg acctgctcca ccccaccaca
     2341 tcatacgacc tgccatacta gaaagcatgg tgatagcact agcaagggca actgcaatag
     2401 tcgaaatacg gatatcataa ttacgaatat gactgacttc atgtcccata acagcttcta
     2461 gttcttcacg attcatgata gctagtagac ctgaagtcgc agcaacagcc gcattttgag
     2521 gattagaacc tgtcgcaaag gcatttaagg ctggatcatc aatgatgaaa acacggggca
     2581 taggaatctg agcgaccaga gccatatctt ccactacatg gtagaggtct ggtgccgttt
     2641 gctcatccac ctcacgcgct ccattcatgg acatgacaat ctctgtcgat tgaaaaatca
     2701 tagacaaagc gtagataaag ccgataatca gtgcgataac caaaccacca agtccagatc
     2761 ttataaagag ataaccaacc gcataaccaa caagagctaa gagtaggaaa aataccagca
     2821 acaaaatcca ggtttttcgt ttattgcttg caatttgatc aaacaacatc ttagtcacct
     2881 aaaccgctaa aatcaacttt aggaaccgac ttttcctctt caggtgtttg aaggaaatct
     2941 gccgctttaa atccaaacat tccagcgata atattgctcg ggaaagtttc taattttaca
     3001 ttgtagttgc tgacaacact gttatagagt tgacgagagt aagaaatttt attttctgtg
     3061 tttgtcaact cctcttgcaa tttaacaaag ttagcactag ctttcaaatc tggatagctt
     3121 tctgcaactg caaaaatacc tgaaacttga cgagtgaggg catcactggc tttcatagct
     3181 tctgctggtg aagtcgctgc cgccacttgg ttacgtagtt ctgccacctt ttcaagggta
     3241 gaaccttcat atttggcata accttttaca gtctcaatca agtttggcaa gaggtcattt
     3301 cgacgtttca actgaacatc aatctgactc caagcctcct tggtttgcat acgattttta
     3361 accaaaccgt tatagctaac aatcacaaaa ataacaataa gagcgataac tccaagaata
     3421 atccaagtca tgatataagt cctttctact tttagattag taccagtata tcaaattttt
     3481 aatgattgtg gtaaaataag atgatactaa agaaggaaat aactatgaaa ccagaaacat
     3541 tttacaactt gcttgccgag cagaatcttc cactttcgga ccagcaaaaa gaacaatttg
     3601 aacgttattt tgagctcttg gtcgagtgga atgagaagat taatttgacg gcgattacgg
     3661 acaaggaaga agtttatctc aaacattttt acgattcgat tgcacccatt cttcaaggtt
     3721 tgattcccaa tgaaactatc aaacttcttg atatcggggc tggggcagga tttcctagtc
     3781 taccaatgaa aattctctat ccggagttag atgtgaccat tattgattca ctcaataagc
     3841 gcatcaactt cctacaactc ttggctcaag aactggattt gaacggagtt catttctacc
     3901 acggacgtgc cgaagatttt gcccaagaca agaacttccg tgctcaatat gattttgtaa
     3961 cagctcgtgc ggttgcccgt atgcaggtcc tatctgaatt gactattccc taccttaagg
     4021 ttggtggcaa actattagca ctcaaggcta gcaatgcgcc tgaggagttg ttagaagcta
     4081 agaatgccct caatctcctt tttagtaagg tcgaagacaa tctcagctac gccctaccga
     4141 atagagatcc gcgctatatc acagtggtag aaaagaaaaa agaaacacca aataaatatc
     4201 cacgtaaggc tggtatgcca aataaacgcc cactttaaat tttttagtaa acaaatgttt
     4261 acaaaatcag cctcgctctt ttatttctag gctcgggaaa aaatgattta caaaatcagc
     4321 ctcgctcttt tatttctagg ctcgggaaaa aatgatttac aaaatcattt ttttctgcta
     4381 tactatccta agcaaaggtt tttaatgtca tcccgtgagg tgacgaagac gcagaaatat
     4441 ttgaaactct ttaaagtcta gattttagag aagtcttact ctgagggcct attgctgtaa
     4501 aataatgggc tcttttctga tgcccaaaag tgaggtttat atgaaacaag aatcaactgt
     4561 tgatttgtta ctagacgttg accaacgtcc ttcggatgga aaaggaattc tccttagctt
     4621 ccaacacgtt ttcgccatgt ttggtgcgac catcttggta ccattgattt tgggaatgcc
     4681 tgtatctgtt gccctttttg cttcaggtgt tggaacactc atctacatga ttgctactgg
     4741 ttttaaagtt ccagtttatc taggttcttc atttgccttt atcacagcta tgtcactggc
     4801 tatgaaagaa atgggggggg atgtatctgc tgcccaaaca ggggttatct tgactggttt
     4861 ggtctatgtc cttgttgcta ccagcatccg atttgtagga acaaaatgga ttgataaact
     4921 cttgccacca atcattatcg gtcctatgat catcgttatc ggtcttggac ttgcaggttc
     4981 agctgttacc aatgcaggtc ttgtagcaga cggaaattgg aaaaatgctc tggtagccgt
     5041 tgttactttc ctaattgctg cctttatcaa tacaaaagga aaaggcttcc tacgaatcat
     5101 tccattcctc tttgccatta tcggtggtta ccttttcgca ctaactcttg gcttggttga
     5161 ctttacacca gttcttaaag ccaactggtt cgaaattcct ggtttctact tgccatttag
     5221 cacaggtggt gcctttaaag agtacaatct ttactttggt ccagaagcca tcgctatctt
     5281 gccaatcgct atcgtaacaa tttctgaaca tatcggagac catactgttt tgggtcaaat
     5341 ctgtggccgt caattcttaa aagaaccagg tcttcaccgt actcttattg gtgacggtat
     5401 cgcaacttct gtttctgcct tccttggtgg accagccaat acaacttacg gagaaaatac
     5461 aggggttatc ggtatgactc gtatcgcttc tgtctcagtt atccgtaacg ctgccttcat
     5521 cgcgattgcc ctcagcttcc ttggtaaatt cactgccttg atttcaacta ttccaaacgc
     5581 tgtacttggt ggtatgtcaa tccttctcta tggggttatc gccagcaatg gtttgaaagt
     5641 cttgattaaa gaacgtgttg atttcgctca aatgcgaaac ctcatcatcg caagtgctat
     5701 gttggttctt ggacttggag gagctatcct taaacttggt ccagttacac tttcaggtac
     5761 tgccctttca gccatgacag gaatcatctt gaacttgatc ttgccatacg aaaataaaga
     5821 ctaagagtct aaatacacct aatccactca gacagctgag tggatttttc gtatatcata
     5881 ataaaagtgt cttaacaaaa ttattaaaat caaaaaacgt ataatatcag atattctaaa
     5941 accttgatac tgtacgtttt atcatagaaa tttttacttt attttctcat caaatgagat
     6001 ttgcatcaat ctcttgtctt acttgcgttt cttcttcgct ttcttcattt tgttagccat
     6061 acgtttcatg gactgtttca tggcaaattc accaatttta cctttcaaac cgccaccaaa
     6121 catctggctc atatctggca ttcctgctcc tccgagagct gataagtcag gcataccgcc
     6181 ttgtcccatc attccttcaa gggcagacat atccattcct cccatatttg gcatattttt
     6241 aggaaggtta tttggattaa tccccatttg cttcatcatt ttattcatat ccccagacat
     6301 aacaccctgc atgagctgtt tagcctggtt aaagtccttg ataaatttat tgacttcgac
     6361 gaatgtattt ccagaaccag cagcaatacg acggcgacgg cttggattta acaaatctgg
     6421 gttttcacgt tcttcaggtg tcatcgaaga cacaatggca cgtttacgag caatctggcg
     6481 ttcatccacc ttcatgtttt gaagtgctgg attgttggcc atacctggaa tcatcttgag
     6541 caagtcttcc atcggcccca tattttgcac ctgatctaat tgatcgatga aatcattaaa
     6601 atcaaaggtg ttttcgcgca tcttctcagc catttcaagg gctttttgtt catcgtattc
     6661 ctgagaagct ttctcaatca aagtgagcat atcccccatg ccaaggatac ggctagacat
     6721 acggtctggg tggaaggttt cgatatctgt aattttttca cctgtaccag tgaacttgat
     6781 tggttttcca gtgatgtgac gaaccgatag agccgcacca ccacgagtat cgccatcaat
     6841 cttggtaagg atgaccccag tcacttccaa ctgagcatta aactcacgcg caacattggc
     6901 tgcttcctga ccaatcatgg catcaacgac gaggagaatt tcatttggtt gagccaatgc
     6961 tttcacatca cgaagctcat tcatgaggag ctcatcaatt tgcaaacgac ccgcagtatc
     7021 aatcaagaca tagtcgttat gattagtttg ggcttgctcc aaaccttgac gtacaatctc
     7081 aacagctggt acttctgttc caagtgcaaa gacaggcaca tcaatctgtt gtcccaaagt
     7141 cttaagctgg tcaatggcag ctggacgata aatatccgcc gcaatcatca aaggacgagc
     7201 attttcttct ttcttgagtt tgttggccaa tttaccagca aaggttgttt taccagcccc
     7261 ttgtaaacca accatcatga tgatggttgg aatcttaggt gacttgataa tttctgccgt
     7321 atcagaacct aaaacggctg tcagttcctc atcaacgatt ttaataatct gttgcgcagg
     7381 attaagtgta tcaatgacct catgcccgac tgcacgctca cgaactttct tgataaagtc
     7441 ctttacaaca ggcaaagcaa cgtcggcctc aagcaaggcc aagcgaattt ctttggttgc
     7501 ctcttggaca tcagattcag agatttttcc ttttttacgt agacttttaa agacgttctg
     7561 caaacgttct gttaaacttt caaatgccat ttttcttcct cttattctct attatcaatg
     7621 cttgttaaaa tttctatctg ctcctgcaga aagttatcct tgggatagcg ctccaaaatc
     7681 tgatcaaaaa tctgactgcg gacaatatag tccgagtaca tgtgcaattt catctcataa
     7741 tcttccagaa tcttttctgt tcgcttgata ttgtcataga cagcctgacg actgacaccg
     7801 aactcctcgg caatttcagc aaggctgtaa tcatcagcgt agtagagctc gatataattc
     7861 atttgcttat ctgtcaaaag cgccgcataa aattcaaaga gcgcattcat acgattggtt
     7921 ttttcgattt ccataacttt tattatacca aaaattagcc taatctacca cactaggaag
     7981 ccgatccaag aagatagata gctaaatttg aaaaagacat aagcctagcc ccaagtaatt
     8041 tccaattgat agctggcaaa gggatgtccc tcttgatttt gtagttgata atctagttca
     8101 atcttttgcc catccacttg ataacgattc gtttggataa taaactcctg catacccata
     8161 ggtgtgggaa tataggctaa actatcacta tcctttagaa agcgcataat ggtcttggga
     8221 ttggaaaatc gactcatcac aagttcttga ccatgaaatt taatcactac tttttccttt
     8281 tcctcattat agaaaagcag gtagctataa tctccttttt catgcacttc cacatcataa
     8341 agctggtcaa tcacttccaa ctgctcatca aactgaattg tatttcgcat ccgaatcttc
     8401 acatcaggcc ctctttcttg tctcttgtcc tactatttta ccaaaaagag caggattttg
     8461 ctataatggt catatgaacg aaaaagtatt ccgtgaccct gttcacaact acatccatgt
     8521 caataatcaa atcatctatg acttgattaa tacaaaagaa tttcagcgtt tgcgccggat
     8581 caaacaactg ggaacttcca gttatacctt ccacggtgga gaacacagtc gcttctctca
     8641 ctgtctagga gtctatgaaa ttgcacgacg catcacagag attttcgaag aaaaatatcc
     8701 tgaggaatgg aatcctgccg agtctctctt gaccatgacc gctgctctcc tacacgacct
     8761 tgggcatggt gcctactccc atacttttga aaatctcttt gatacagacc atgaagccat
     8821 tactcaggag attattcaaa atcctgagac agagattcac caagtcctgc tacaagtggc
     8881 acctgatttc ccagaaaagg tggccagtgt cattgaccat acctatccta ataagcaggt
     8941 cgtgcagctc atttctagtc agattgacgc agatcgcatg gactatctct tgcgcgactc
     9001 ctattttaca ggagcatcct atggggaatt tgacctgact cgaatcctcc gagtcattcg
     9061 tcctatcgaa aatggtatcg cctttcagcg caatggcatg cacgccatcg aagactacgt
     9121 cctcagtcgc taccagatgt acatgcaggt ttatttccac cccgcaacac gcgccatgga
     9181 agttctccta cagaatcttc tcaaacgcgc caaggaactc tatcctgagg acaaggattt
     9241 ctttgcccga acttctccac acctcctgcc tttcttcgaa aaaaatgtga ccttgactga
     9301 ctatctggct ctggatgatg gcgtgatgaa tacctacttc cagctttgga tgaccagtcc
     9361 tgacaagatt cttgcagatt tatcgcatcg ctttgtcaac cgcaaggtct ttaaatccat
     9421 taccttttca caagaagacc aagaccaact cgctagcatg agaaaattgg ttgaggacat
     9481 cggctttgat cccgactact atactgccat tcataagaac tttgacctcc cttatgatat
     9541 ttaccgtccc gaatctgaaa atccaaggac acagattgag attttacaaa aaaatggaga
     9601 actggcagaa ctctctagcc tgtcccctat cgtccaatcc cttgctggaa gtcgccacgg
     9661 agataatcgc ttttatttcc cgaaagaaat gttagaccaa aacagcatct tcgcaagcat
     9721 cacccagcaa tttttacact tgattgagaa cgatcatttt accccaaata aaaactaaaa
     9781 gaggaaattt atgagtatta aactaatcgc cgtcgatatt gacggaaccc tagtcaacag
     9841 ccaaaaggaa atcactcctg aagtcttttc tgccatccaa gatgccaaag aagctggtgt
     9901 caaagtcgtg attgcaactg gccgccccat cgcaggtgtt gccaaacttc tggacgactt
     9961 gcagttgaga gaccaaggtg actatgtggt gaccttcaat ggtgcccttg tccaagaaac
    10021 tgctactggc catgagatta tcagcgaatc cttgacctat gaggattatc tggatatgga
    10081 attcctcagt cgcaagctcg gtgtccacat gcatgccatt accaaggacg gtatctatac
    10141 tgcaaatcgc aatatcggaa catacactgt acacgaatca accctcgtca gcatgcctat
    10201 cttctaccgt acccctgaag aaatggctgg caaggaaatt gtcaagtgta tgttcatcga
    10261 tgaaccagaa attctcgatg ctgcgattga aaaaatacca gccgaatttt acgagcacta
    10321 ctctatcaac aaatctgctc ctttctacct cgaactcctt aaaaagaatg tagacaaggg
    10381 ttcagctatt actcacttgg ctgaaaaact cggattgacc aaagatgaaa ccatggcaat
    10441 cggtgacgaa gaaaatgacc gtgccatgct ggaagtcgtt ggaaaccccg ttgtcatgga
    10501 aaatggaaat ccagaaatca aaaaaatcgc caaatacatc actaaaacaa atgacgaatc
    10561 cggcgttgcc cacgccatcc gaacatgggt actgtaaaag tatcattttt caataagaat
    10621 tgattagcaa taaaatccaa tgaatttttt tagcaaacta tttaatttaa aacaaaataa
    10681 tcataataga gacacaaatt ctgattgtaa caatttttac ctaaacgatt tagaatgtgg
    10741 ccttactcct gggcaactca tactcataga ttggactcaa aaaacaggga gaaattataa
    10801 tttcccaaga tattttaaat actctcttca aattgaccct gaatctacac acaatcaatt
    10861 atacaaatta ggatacttca ctaaaaataa gactttatca tatcttacag tagtagaatt
    10921 aaaaactata ttatctaaac ataatttagc tacttctgga aaaaaagcag aattaattac
    10981 aagaataatt aataatgtta acattgacaa tttagatatt ccgttcgaat ttaaactaac
    11041 aaaagaagca caaaatctta ttatcgaaca tagtgactat atcaaagcat actatgataa
    11101 agacataact atggaagatt attgtaaaga aaaaaacaat atctctttta aagcaacttt
    11161 tggtgatata aaatggagtc tcttaaataa acaagctcat aggaatactg tatcaggaga
    11221 ttttggatgc ttatctaaca cacgaaaggc tcagggaaga catttggaac aagaaggtaa
    11281 tattaaacat gctttaatat attacataga atctttgata attactattt caggattaga
    11341 aaacaatttt tcagccactg attatccagt atattatccc gattcgatac ctgactactc
    11401 actaaaacat attcaaacat taatggaatc attatctgat gacgattatg attttgcttt
    11461 tgatgaagca ttatttcgct tctcaatttt gaatgcaaat cattttttat ctaaggaaga
    11521 tattgactat ttaagagtta atttacctcg ttccactgct gaagaaataa acaattactt
    11581 aaagaaatat gaatgttata gtcctttaaa taatttagaa cttgacgatt ttgaataaat
    11641 tgactataca aacatttata tactcgatat agtctcaatt ttatctgatg attgcccaaa
    11701 tttttcaata ataaaacgca taatattatg gagacaatcc cctatattat gcgttctttt
    11761 aatatcaaag actttttgac aaacttcttc gatatctaat tactgccccc tgcaggaatc
    11821 gaacctgcaa ctactcctta ggagggagtt gttatatcca ttgaactaag ggagctagat
    11881 aaaaactctg ctgaaaagca gagtttttta gtcgaattaa cgacggattt ctttgatacg
    11941 agctgcctta ccttgaagag cacgcaagta gtacaatttc gcacgacgta ctttaccgta
    12001 acgaacaact tcgatttttt caacacgtgg agtgtggatt gggaagatac gctcaacacc
    12061 tacaccgtta gagattttac gaactgtgta gttttctgag attccagcac ctttacgtgc
    12121 gataacaaca ccttcaaaaa tctggatacg ttcacggtta ccttcgacaa ctttcgcatg
    12181 tacacgaaca gtgtcaccag gacggaatga tgggatatct gtacgaagtt gaccttcagt
    12241 caagctttgg attaatggat tcattttatt ctcctatctt tgtcaatctt gaggaatctt
    12301 cctcagcgga taaactgtat ttttgtgcgt ccattacaca caagatatag tttaccaaat
    12361 ttctaaaaaa aagtaaagaa atttatagca aaattcctaa aaaaatcgca accaaaccac
    12421 ctatgtaagt caaagtcagg taagaataaa agaccttctt atcacttagc agtctttgaa
    12481 gttcgtcatt caaggtcgaa aaagttgtta aaccgccaca aaatcctgtt gctagaatag
    12541 catatacttc cttagactct acatgattgt agaatactcc aattaaaaaa caacctaaaa
    12601 gattagctat gagcgttccg agtggcaatt tagaagcttg attatagcga gaaaagaaat
    12661 accgtacgag tgctcccaag ccacaggcga ttgcaagata gacgattacc atttcttcct
    12721 ccccaaatag taagctaaaa gcaggcctcc accgatactc aaaagcaaat acagtatcaa
    12781 actaagataa cgccctgtat caagcagctt cacagtatca agcattaggc tggaaaaagt
    12841 tgttaaacct ccgcaaaaac ccgtccccag tgctaaaatc aagcccttac tagtcccctt
    12901 atagaccaga tagcccttga caagataaat caagcagaaa attcccagat agttgacaaa
    12961 gagagtgccc caagggaaat ctggactggc tggtaaccaa gtggaaacta ggtagcggac
    13021 aagtccgccc aacatagcag ctagaaaaat ccctagcgga taaaattgtt cttttttcat
    13081 ttgatatttt gatcctgata atcacgcgaa cgtttgagta tgtccgaaaa agtcgcgaca
    13141 atagtctcct gatagcgctt gtcctctaca cgacttctaa ccttttcaaa aataactgct
    13201 tctctcttag tatctaaaat cggtttccct gaagccttct tataagcgac aactccttca
    13261 actaagtgca ttcgttcttc taacagtttg acaatttggt cgtcgatttg atcgatttct
    13321 tgacgaataa tatctaaatc catacagtct cctcctttat ttgaattatt gtatcaaaaa
    13381 gccatcaatt aggctagtaa aatcccaact ctcgataaga aaaatgcact gattgattac
    13441 atcagcgcac ttttatgctt atcctacttt agcagccaat tcttctgcga attgttccaa
    13501 gcgttcaata tcttcttcct cggcagaaag atcaacttta acacactctg aacctttttc
    13561 tgctcctgtt gacacaaaga cacggtcaaa gtcatcaaca gccttacaaa attcatcgta
    13621 gaaggtatct cctgagccga ccactccgta gattttgcca ttcaagttga gatctgctag
    13681 gtcttcgtag aagtccatca tctcatctgg caattcacca tcaccataag tataggtcgc
    13741 aacgatagcg atgtctgctt ccaagaagtc tgaagcgtca acagttgtac attcatcaac
    13801 atcaacatcc aagcccaagt cacgtaattt gtctgctaca atatctgcaa tttcttcggt
    13861 attaccggtc atactggcaa atacaatttt tgctaatgcc atatcgtcct cctcaattta
    13921 tctttctcca ttatatcaca tctttttcat tttaaaaata aaaattcttg tgctacaatg
    13981 aaatgagaaa gaattgaggt tatttatgga gatttgccaa caaattttag aaaaaattaa
    14041 agaatacgac accattatca ttcatcgtca tatgaaacca gaccctgatg ccttgggaag
    14101 tcaggtggga ttgaaagcct tgctagaaca tcatttccca gaaaaaacca tcaaagccgt
    14161 cggttttgat gaaccaactc ttacttggat ggctgagatg gatcttgttg aagatagagc
    14221 ctaccaaggc gcacttgtca tcgtctgtga tacagctaat actgctcgta tcgatgataa
    14281 gcgctatagt caaggtgatt ttctcattaa gattgaccac catccaaatg atgatgtata
    14341 cggtgacctg tcttgggtcg atactagttc aagtagcgct agcgagatga ttaccctatt
    14401 tgcccaaaca acccaactag ccttggcaga tcgcgatgct gagttgctct ttgcaggaat
    14461 tgtcggtgat acaggtcgct tcctctaccc ttctaccact gcacggactc ttcgcctggc
    14521 tgcttatttg agagaacata actttgactt tgcggctctc actcgcaaaa tggacactat
    14581 gagctacaaa attgctaaac tgcaaggcta catctacgac catctggaag tggatgaaaa
    14641 tggtgctgct cgcgttatcc tgagtcagaa aatcttgaaa caatacaata taaccgatgc
    14701 tgaaactgcg gccattgtag gtgcacctgg acgcattgac agagtgagtc tctggggaat
    14761 ttttgtcgaa caggctgatg gccactaccg agttcgctta cgcagtaaag tccatcctat
    14821 caatgaaatt gccaaggagc atgatggtgg aggccaccct ctagcaagtg gtgctaattc
    14881 ctatagccta gaagaaaacg aaatcatcta ccaaaagtta aaaaacttgc ttaaaaactg
    14941 ataaaatact tgccaaactt ttcagaatct gatagactag tatagtaaca atctatggct
    15001 cgcaaagaga ccatggcaga aaggaaatat tgcaaaatga aaaaagatat ccatccagaa
    15061 tatcgcccag ttgtcttcat ggacacaact actggttacc aattccttag cggttcaaca
    15121 aaacgctcta acgaaacagt tgagttcgaa ggcgaaactt acccattgat ccgtgtggaa
    15181 atttcatcag actcacaccc attctacact ggacgtcaaa agttcactca agcagatgga
    15241 cgcgtggatc gtttcaacaa aaaatacggt ctcaaataat gataagagaa cagttttggc
    15301 tgttcttttt tgtttcttga aatcaactgc tgttttcatg ttccagactc atctgtaggt
    15361 tcgatttcca tgctactagg caggaaggaa atagctgttt caacacgtcc ataatgagct
    15421 atactattgt cacgaaccac actttcattg atggtccaag tggaattcat tttcttaaaa
    15481 gcttctcgga ctttttccaa atctttggag gcaatggcct gctctaaggt ttcaaaacga
    15541 ggacttatac tcatctgctt tcaaaaagca ttctagtcca tctccgatta ccgatggact
    15601 ttatcacctc cttctccagt ccttgtatga catcttg
//
LOCUS       DBERYS010000042        15157 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.042, whole genome shotgun sequence.
ACCESSION   DBERYS010000042 DBERYS010000000
VERSION     DBERYS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.042"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(209..1177)
                     /locus_tag="ACL8LA_001595"
     CDS             complement(209..1177)
                     /locus_tag="ACL8LA_001595"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS1813334.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1322..2105)
                     /locus_tag="ACL8LA_001596"
                     /pseudo
     CDS             complement(1322..2105)
                     /locus_tag="ACL8LA_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
     gene            complement(2130..2627)
                     /locus_tag="ACL8LA_001597"
     CDS             complement(2130..2627)
                     /locus_tag="ACL8LA_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGS1813335.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2700..4061)
                     /gene="radA"
                     /locus_tag="ACL8LA_001598"
     CDS             complement(2700..4061)
                     /gene="radA"
                     /locus_tag="ACL8LA_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGS1813336.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4075..4590)
                     /locus_tag="ACL8LA_001599"
     CDS             complement(4075..4590)
                     /locus_tag="ACL8LA_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1813337.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4592..5035)
                     /locus_tag="ACL8LA_001600"
     CDS             complement(4592..5035)
                     /locus_tag="ACL8LA_001600"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGS1813338.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5340..5489
                     /locus_tag="ACL8LA_001601"
     CDS             5340..5489
                     /locus_tag="ACL8LA_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813339.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            5631..5810
                     /locus_tag="ACL8LA_001602"
     CDS             5631..5810
                     /locus_tag="ACL8LA_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813340.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6030..6395)
                     /locus_tag="ACL8LA_001603"
                     /pseudo
     CDS             complement(6030..6395)
                     /locus_tag="ACL8LA_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
     gene            6415..6582
                     /locus_tag="ACL8LA_001604"
     CDS             6415..6582
                     /locus_tag="ACL8LA_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HGS1813341.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(6737..6940)
                     /locus_tag="ACL8LA_001605"
     CDS             complement(6737..6940)
                     /locus_tag="ACL8LA_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813342.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(6963..7154)
                     /locus_tag="ACL8LA_001606"
     CDS             complement(6963..7154)
                     /locus_tag="ACL8LA_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGS1813343.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            7725..8093
                     /locus_tag="ACL8LA_001607"
     CDS             7725..8093
                     /locus_tag="ACL8LA_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1813344.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            8093..8326
                     /locus_tag="ACL8LA_001608"
     CDS             8093..8326
                     /locus_tag="ACL8LA_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813345.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            8326..8589
                     /locus_tag="ACL8LA_001609"
     CDS             8326..8589
                     /locus_tag="ACL8LA_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS1813346.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            8602..8982
                     /locus_tag="ACL8LA_001610"
     CDS             8602..8982
                     /locus_tag="ACL8LA_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGS1813347.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            8999..10069
                     /locus_tag="ACL8LA_001611"
     CDS             8999..10069
                     /locus_tag="ACL8LA_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HGS1813348.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            10130..10477
                     /locus_tag="ACL8LA_001612"
     CDS             10130..10477
                     /locus_tag="ACL8LA_001612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813349.1"
                     /translation="MEELPNGKYKFFERYKDPYTEKLKKVSVTMEKKTPQARNQAAIL
                     LQEKIKQKLGEKQHSVSNITFEKLYEEFEENWKHGVKNSTVYASKNVKKEILKQIEGD
                     YLVRNLIDVYYKK"
     gene            10567..11265
                     /locus_tag="ACL8LA_001613"
     CDS             10567..11265
                     /locus_tag="ACL8LA_001613"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1813350.1"
                     /translation="MNYIDTNEMLFVETPRKVITSDELRKKNTKYLDQKEFKLFIQNL
                     KDEALCDYRITKYIRIAKVLFLTGMRYGELAALNYKEDIDFSKKTIHIKHTYDFRQKE
                     RTTPKTIKSDRVITAPQKVLDIIKEQIIENATNGFDTDFIFINTLGEPITNARVICAL
                     KRHGQKIGIEKNITTHTFRHSHISLLAELGIPLTAIMDRVGHSDSKTTLEIYSHVTQK
                     MVSDISSKLDKIKF"
     gene            complement(11348..11441)
                     /gene="ffs"
                     /locus_tag="ACL8LA_001614"
     ncRNA           complement(11348..11441)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACL8LA_001614"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(11474..11941)
                     /gene="tadA"
                     /locus_tag="ACL8LA_001615"
     CDS             complement(11474..11941)
                     /gene="tadA"
                     /locus_tag="ACL8LA_001615"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGS1813351.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(12142..13428)
                     /locus_tag="ACL8LA_001616"
     CDS             complement(12142..13428)
                     /locus_tag="ACL8LA_001616"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGS1813352.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(13659..13895)
                     /gene="comW"
                     /locus_tag="ACL8LA_001617"
     CDS             complement(13659..13895)
                     /gene="comW"
                     /locus_tag="ACL8LA_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HGS1813353.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            14161..14958
                     /locus_tag="ACL8LA_001618"
                     /pseudo
     CDS             14161..14958
                     /locus_tag="ACL8LA_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14993..>15157)
                     /locus_tag="ACL8LA_001619"
     CDS             complement(14993..>15157)
                     /locus_tag="ACL8LA_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1813354.1"
                     /translation="LCEEFGHKLLPLPPYSPEYNPIEKTWAYIKKNLKKVLPSCNTFY
                     EALFSCSCFN"
BASE COUNT         5049 a         3348 c         2507 g         4253 t
ORIGIN      
        1 cctactagaa ttctccttag aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg
       61 gtcgctgaaa cttctgatga cgtatagtca tatttcttct ctttttatta tatcacagtt
      121 ttaaatctag ctttactaga ttcaccgcta ctatccattt attcggaaaa aagacgaaaa
      181 aacctgagaa tcatctcagg cttggtcatt aaattttttt ctcaatatcg aaaagtggag
      241 aaagtggtcg tttttcatga atacgtacga tagcatcccc taggagatga gcgattgaaa
      301 tctgctcaat cttatcaatc aaacgctctt ctggcagata gatggtatcc aaaacaacca
      361 atttcttaat agctgatttt tggatattgt ccgtagcagg accagaaaga actgggtgcg
      421 tacagcttgc atagactgca acagcaccag cttccgcaag agcatctgcc gcatgacaaa
      481 tcgttccagc ggtatcaatc atatcatcaa tcaagataca agtcttgcct tcaaccttac
      541 cgatgatgtt cataacttca ctagtattca tcttatcaac gctacgacgt ttatcaataa
      601 tagcgataga tgttttcaaa aattctgcca acttacgagc acgagtcacc cctccatggt
      661 ccgggctgac aaccacatag tcagaaccaa ccataccacg ccgctcaaaa taatctgcaa
      721 ttagaggagc acccatcaaa tgatccacag gaatatcaaa gaatccttga atttgcgcag
      781 catgcaagtc gatggtcaat aaacgatcca ctccagctac ttcaagcata tttgcgacaa
      841 gttttgaagt gattggctca cgcgctctcg cctttctatc ctgacgtgca tacccatagt
      901 aaggcatgac aacattgaca gactctgcac tcgcacgctt caaagcatct accataatca
      961 aaatttcaag cagattgtca tttacaggcg aactagttga ttgtaagata aagacgtgtt
     1021 tcccacggat tgattcttca atgttgacct gaatctctcc atctgaaaat tggcgaacac
     1081 ttgatttccc caactctatc ccaatctcct gcgccacacg ttctgccaat tctttattag
     1141 aagaaagggc aaacagcttt aaatcagaaa aagacatgat ttcctccggt atatatgtat
     1201 cgcttgtgct tttcacaaga ttttccatct accattgtag cgctttttgc actatttttc
     1261 aatcaaaaat aaaagaaggg caccatattt gtacccttgc atcattcttt tgaaaaatat
     1321 tctaggtcat caactcattg tgtttctcaa caaagcaata agcatgataa taaccataga
     1381 gagcaatagc cgtaaccact ggaatcgcta aaggcaactc tgtttccaac tccacaaaag
     1441 gagagttaaa caataagacc ctgtttcttg ccaaccttct gtcctttata ggctctgtaa
     1501 agcaagtaaa cacctactac agctagacct gaaaaagtcc agtgagaggc aattcctgag
     1561 atgatacgct ctaaaattcg cgaaatagta aagtcaaagc cctctggcaa atccgtacga
     1621 atataaccaa tatcctcaat catttggaat cccaaaccgg aggcaattcc aagtaaaaac
     1681 aaaaatttta attttcgcac aggaatcaaa gccaaaacaa aaacaagtgg caataatttc
     1741 aagggttctt ctaccaaagg agccacaata gcactttcaa aggcatttaa aaatggacta
     1801 tctgggaaaa gaacccccag taaatcatgt atataagtat tagcaaaact agacaaccag
     1861 cctgaaagga acatccctcc caataaagac agaatcaaaa ccttctttgg caattcccat
     1921 tttttcccaa tacggaagag aaaataaaga gccggaatca tgtaaaagag agctagaaag
     1981 atagaaactc ccattagtcc atattccgca cctgacctcg aaccgtccgt atagtagatg
     2041 gtttcatact gtaaaccaat acatagcaat aaaataaaaa taaataaaat actgcttttc
     2101 ttcatacact ttctttctaa atgaagtatt tataattcta cgactgtcat acttcctgta
     2161 tcaacattgt aaatagcacc agagataatg acatcgtctg gtattagggg agactcgata
     2221 agcagttgca tatcctcgcg tacactctct tctatatctt ggaagggcaa gaagtcctgg
     2281 tctgacacat ctacacctaa ttcctctttt aaatactcct gaaaaggttc attttcaaag
     2341 gtctgagcac cacagtctgt atggtgcaat accacaatct ctcttgtccc catttgttgc
     2401 tgggaaataa ctagcgaacg aatcatgtct tcagtcactc gaccacctgc attccgcaag
     2461 atatgagcat ccccaagtgc caagcccaga gcttgcgcaa cgtgcaaacg tgagtccata
     2521 caggtcacaa tggctactct ggttttaggt ttaagtggca gatttaactg cccatgtagg
     2581 gcaacataag cctgattggc ttgcataaac tgttcaaaat acgacacgat tccctccttg
     2641 aaaatttgat agtcaaatat ttctcctatc ttatcatttt taagagaatt tgtcacggat
     2701 tatgcaaaga cctttttcaa gacttcctga atcgttgtca cgccaatgac ctgaatttcc
     2761 ttaggcagag tgattcctgt caaggaattc tgaggtacat aaatcttagt aaagcccagt
     2821 ttagcagctt cgttgatgcg ttgctcaata cgattcacgc gccgaatctc tcctgtcaag
     2881 cccagttctc cgacaaaaca ttcctgagga ttagttggct tgtctttgta gctcgaagca
     2941 atagcaactg caacagccaa gtcaatggca ggttcatcca atttaacacc accagcagat
     3001 ttgagatagg catcctgatt ttgcaagaga agccctgccc gtttttccaa aacagccata
     3061 atcaagctag cacggttaaa atcaagtcct gtcgtagtac gcttggcatt tccaaacatg
     3121 gtcggtgtta ccaaagcctg aacctccgcc aaaatcggac gcgtcccttc catggttaca
     3181 acgatggagg aaccagtcgc cccatccaaa cgctcttcta ggaaaacttg actcggattg
     3241 agtacctcaa ccaagccgcc cgactgcatc tcaaaaatcc caatctcatt agtggaacca
     3301 aaacgatttt tgaccgctct caaaatacga aaggtgtggt gacgctcccc ttcaaagtaa
     3361 agcaccgtat ccaccatatg ctccaacata cgaggcccag ccaaggttcc ttctttggtc
     3421 acatgaccta cgataaagat ggcaatgtta ttggtcttgg ccaactgcat gagttcagcg
     3481 gtcacttcac gtacctgaga aacagacccc tgcacccctg aaatctcagg agacatgatg
     3541 gtctggatgg aatcaataat gagaaagtct ggctggatac gctccacttc tgcacgaaca
     3601 ctctgcatat tggtctctgc atagagataa aactcactat caatatcacc taagcgctct
     3661 gcacgtagtt taatctgctg ggcagactcc tccccactga catagagaac tgtccccact
     3721 tgggacaact gggttgagac ttgtaggaga agagttgatt tcccaatccc aggatcccca
     3781 ccgatgagga cgagacttcc tggtaccact ccgcctccaa gcacacggtt gaattcctcc
     3841 atctccgtct tggttcgatt gacattgatg gaagtcacct cagctagttt catgggcttg
     3901 gttttctcac ctgtcaagga cacacgcgca ttcttgacct cggcaacctc aacctcttcc
     3961 acaaaagaag accaagaccc acagttgggg caacgtccca gatatttagg ggaattatac
     4021 ccacaatttt gacatacaaa tgtcgctttt ttctttgcga tgacaaacct ctttctatat
     4081 ctctaactca cactcaatca cttggcaaaa atcaatcttc tcatttggca caaactggcg
     4141 catgagcatt cgatgagcaa caactaccac agtctgatgt tctcgatact tagacataca
     4201 ttctagaaac cgagacttca tttccgtagc tgtctcatat tgaataggac tattaggaag
     4261 caactccccc ttgttttcta aaaacagtct tctagctgtt tcaaagtttt ctattcctgt
     4321 tttatagacc tgccattcat gtaataaagg ctctactctt aaaggaagac ccgtagcaca
     4381 gaccacatac gaagccgttt ctaaagctct tgtgactgca gaagatacga ttatttcagc
     4441 tgacgagagt aaaggatttt tgctcaattt ctggacttgc tgccgtccca tctcagacaa
     4501 gggtgccaaa tctatcccaa atcctatata agaacgctcc tctaactcac ggtaatctgg
     4561 ctccccatga cgtacaaaga taatcttcat tctagtgccc tgtcgatcca aatccaccag
     4621 ttcgaacgcc atcagctgca tctccatctg caattaagaa agtagcaaaa acagcctgga
     4681 caatacgttc cccaacttca agaacaacct cttgatctgt gatattcttc atctgcgcaa
     4741 aaatatgccc ttcatttcca ggatttccat aataatcccc atcaatgacc ccaactgagt
     4801 taattaaaac caagcccttc ttacgaggat ttgaagaacg atcatagagg tagagaacct
     4861 cagtcggctg catataagcc ttaacccctg tcggaaccaa gacaatctct cctggcgcaa
     4921 caactgtacg cacagcaacc tttaagtcgt aaccagccgc atgcgctgtc tcacgcttgg
     4981 gcaataaatt ttcatctgta aaactcgaaa ccaattcaaa accacgaatt ttcataattt
     5041 tctcttttct attatcattt attctagatt attctatctt atttattcgg aaaaagcacg
     5101 aaaaaaagag cacacaacaa ttataggcga tacgataatt tacactgttt cacaatacgt
     5161 tgaaatttag agctttaaag cgagggcaaa gttgattttt tacatcgttt tacagaggtt
     5221 tacgacactt ttgccccttt tttgcccctt tgtttttcaa aaaaacttca tcaaatcgct
     5281 tgactttcac ggtttaccgt gatataatat aatcaagata aggaaaggag gtgaggaagt
     5341 tgaacaaaga agattggctt aggttactgg aaaaggcgat agataatatc cctgaaacgg
     5401 taactgctat agcaagtctg gtgactgcaa taacggtcgc aaggcaaaac aaaaagcgta
     5461 aaccgaaatc ccgtcaaaga aaaaggtgaa cgctaagagg ttggggcgaa agcccctcac
     5521 acctctattt tatcaaatga aaagaggaaa agcaatggtt agtgcaatag ctatttttat
     5581 aattgcggtc aatgtatata tttatctaaa aaataaaaag gacaaataag atgagagaag
     5641 ttattcaaga attgttagac agttcgatgt ctacatctgc tatttcccaa ggtgctggag
     5701 ttccatggac tactgtttct gacctcagaa aaggaaaaac aagcatggac aaaatggcac
     5761 ttctcacagc agaaaaactt tatgaatttg ctacaactga taagcagtga tttcggtcac
     5821 tgtttttatt tttgacaaaa caccgttttt gacaataatc aaaaaataaa aaggagctat
     5881 tattaacaaa atggcgtttt tggcaataat accctgccat tcctcctccc tattcttcaa
     5941 gaaaacgctt ttttgaagaa tcagagcaaa taaaaaaccg caagcctgag cctgcggtga
     6001 aagaacaatt tagaaagttt cctttctatt tatttaactg taatcaagcc atctggctct
     6061 actgtgaact ctggcttgtc tgccagtgtt ccgtctggtt tgaggtagta ccagcctgtt
     6121 ccgtccgctg actggataaa ggcatttgat accatggcgc cttctttagc gtctaagtag
     6181 taccaagtgt ccttgtactt gacccagcct gtcttcatgg caccttctac atcaaaatag
     6241 taccacttgt cagcgatttt cttccagcct gtggccattt cgcctgattg gtcaaagtag
     6301 taccaattac cgtctgtgtg cttcttccag cggtctgaaa gcatatagcc tgagccatcg
     6361 aaataatacc agataccgtt gattttctca aacttataga aaggaggaga tagtatggca
     6421 aatactccaa taaaacctgg aacagataat cagaaacctg gtcgctatgt agaggtagga
     6481 cctcgtggcg gaaaagttac taatggtcat accgcaacta tcggaaaagg tgatcggctc
     6541 cctccgacat ctgctaaagg caacggttgg aaaaaagtct aatcttcgtt tgcgtacaat
     6601 cgttcaatgg ttgtacgctt tttccataca caaaagcaca ttcccaaaaa atcaatttga
     6661 atccatgctt cggcgtaatc tttcccattg ctggcatagt gagttatata atggtgaatc
     6721 attttattcc ttcctcctat tgagttactt gagaagtatc atgaatggct tcatagctaa
     6781 gacgcttaaa ttcttctgag tctatctgaa aattgatagg ctttttttgt aaacactcaa
     6841 gaaaactagt gtttcttaaa agtttttcaa ctaactcagg gtctgccttt acaaaggtgg
     6901 actctttttg tccactatac ggatatcgtt ttggtctcat tttcctactc ctcaaatctt
     6961 tcctactcaa tcccataatc ttcaataacc tgaagaatga aactgttcgc tcgtggacct
     7021 ttagtcgttc cacttagaat gtttgtcact tcctgtcgtt taaagccgta agcaaccgct
     7081 agagttgctt ttttaatgcc tttctctttc aagaaagcaa taactctttc gcgaccgttt
     7141 gcgatatctg gcatattttc tcctttcttt ttctttcttc tttttctgct ataatataag
     7201 cagaaaggag ttaaccttat gacttttaaa gaatatttac tcaaagcaag caaacgcgac
     7261 atctacgatg atggtaaaga ttttgagaaa tcaatttcag attctcacta atcgagcgtg
     7321 acttttttga tatggcaatc tcagaaattt accaccagcg ctgactacct taatgacttt
     7381 ttcaaggtgg tctttttctt gttctaaatt tttaagaata cttaccatca gcacgttcct
     7441 cctctttact tatttgtaaa taagaaacaa ctaaaaatgt aactattttt ctgcattata
     7501 cttgacaact tacaccaaat cggctaaaat taaagcataa taaaaacacc aataaatcta
     7561 taaataccgt tcgccaaaac atttttataa tttatttctt agttgttttt ttagttgttt
     7621 atcacttaca aaaaacattt tacacctttt gggataatta gtcaaccttt ttacaccaaa
     7681 tttgttaaat ttttttgtga tgtcttagaa aggttgattt aacaatgttt gagacatttg
     7741 aaaaaataaa agaattggca aaaaagcgtg gaaaagctct tggacaagta gaagaagact
     7801 taggttatgg tagaaataca ctctataaga taaaaaattc tacgccaaac gctgaacgta
     7861 tcgcagaaat tgctaattat ttcaacgtat ccaccgacta cctgctcgga cgaacagata
     7921 accctgctat cgctgggagt gatgaatttg cccaagtaaa cggacaaatc atagacttac
     7981 gcaaagcagc agccaacacc atgttatttg acgggaaacc actaaatgaa gatgatatcg
     8041 acttcattac atccgtccta tccgcccact tcaaaagcaa aggagaacgc taatgactat
     8101 cactatcaac ttcacagaaa aaaattccta cattacggac tacctaaaca aacacggtat
     8161 cgacacaacg accatggatt ttgacgactt catggcactc atggaagata tcgaagacgc
     8221 acgagcagcc gaccaagcct atatggagta tttagccgac ccagctactt ataccatgga
     8281 tgaggtcttg gatgaactag gactaactcg agaggatatt gcttaatgta tcggctagat
     8341 attgataaaa aagctctcaa gcaacttaaa aaactagata ccccaaccag aaaacaaatc
     8401 ctatcctggc ttgctaaaaa cattgaaaac acgaccaatc cacgacaaca cggaaaagca
     8461 ctaaaagcca accttgcagg ttactggcga tacagagtag agaattaccg catcatctgt
     8521 gatatccaag acgataaact agtcgtccta gccgtggaaa tcgcccaccg cagagatgtc
     8581 tataaataac gaaggagaac tatgacactc gctaaactct gcgaagaata tcaagtagaa
     8641 ctttgtctct tcgacggttc aaactggcac aatagcggtt tctacaatcc agacacaaac
     8701 gtgctcgcta ttgaccacaa cttgactcct gaacaacaaa tccaagtcgc cctacacgaa
     8761 cttggacaca aagaccacac acgctcagag caccagaacg cccgtctacg ctgtgaaaac
     8821 gaagctgata ggaatatgat ccatcatctc gtaaaagacg ctctagaaaa cttagacgac
     8881 cccacagagt ttgattacct caaattcatg tcttactaca atctaaaaac catgacaaat
     8941 gaaatcatgg ttaaagaaga gtatttagca ttagtaaatt aaaaaaagga gtatcaatat
     9001 ggaaattgat aaagtaaaag ccgatttaaa acaagtcggc aaacgtgtag cagacctcag
     9061 ccaaagcatt acgaatgaag aacaaacaaa gaatgccttc attatgccgt tcttccaagc
     9121 gcttggatat gatatcttca atccacttga atttgtcccg gaatttactg ccgacgtggg
     9181 cattaaaaaa ggcgaaaaag tcgactatgc gatcattttg gatggcgagc ctcaaatcct
     9241 aattgaatgt aaatcaatta cagaaaacct tactaagcac gactctcaac tattcagata
     9301 tttcgtaact actaaatcaa aattcggtat cttgacaaat ggtagagaat ataaattctt
     9361 tactgattta gatgagccaa ataaaatgga cacaactcca ttcttaacaa tcgatgtgac
     9421 tgatatcaaa gaaaatcaat ttactgaaat catcaaattc cacaaagaga attttgatat
     9481 tgataatatt gtttcatcag cttctgaatt gaaatacctc aacaatctta aagcgttcct
     9541 tactgaaaat atcactacac cgtcagatag cttcctcaga tatctaactt ctgaaatcta
     9601 tgaagggcgt gttactcaaa atatcttaac gacattttct cccattattg tgaaagggtt
     9661 taatcaattc atcacagagc gagttaatga aaaattaagt gctgcactta atacaagcgt
     9721 tgaaaccaaa gtaacaaccg atattccaaa agttgaagct gaagctgaag agattgtcga
     9781 agtaactgac gaaattatca caactcctgc tgaattagaa gtttatactg ttgttaaaat
     9841 gcttgctagg gatgtagttt ctccagaacg tgtattttac agagataacc gtagctattt
     9901 caatgttttg gttgacgata acatcaaaaa atgggtatta cgttatcgtt caaattcaaa
     9961 aaaaagcact atcgaaatcc gtgataaagg catcttcccg gtgtctaccc ctctcgaagt
    10021 cgcaaattat gccaatgaaa ttttagaagt aatcaagaaa ttctcataat aaaaaaagcc
    10081 ctctttacta tacccatttt atcaaaaaag tgaggtaaaa tcaatgtgga tggaagaatt
    10141 gccaaacggc aaatataaat tttttgagag atacaaagat ccatatactg agaaattaaa
    10201 aaaagtctca gtaacgatgg agaaaaaaac tccccaagca agaaatcaag ctgcgatttt
    10261 acttcaggaa aagataaaac aaaagttagg agaaaaacaa cattctgttt ctaatataac
    10321 ttttgaaaaa ctatatgaag aatttgagga gaattggaaa cacggcgtaa aaaactcaac
    10381 cgtctacgct tcaaagaatg taaaaaaaga gattttaaag cagatagagg gcgactacct
    10441 agttagaaat ttgatagacg tttattacaa aaagtaatag atcaactatt acaagatggg
    10501 agatctcata actatgtttc taaaatcaaa ttcaagctca atcagataat gaaatttgct
    10561 atcagaatga attatattga tacaaatgaa atgctatttg ttgaaacgcc tagaaaagta
    10621 attacatccg acgaactcag aaagaaaaat acaaaatact tagaccaaaa agagtttaag
    10681 ttattcatcc aaaatttaaa agacgaggcc ctatgtgatt atcgaattac aaagtatatc
    10741 cgaatagcta aagttctttt tcttactggc atgaggtatg gagagctggc agccttaaac
    10801 tacaaggaag atatagattt ttctaaaaag actattcaca tcaagcatac atacgatttc
    10861 agacaaaaag agagaactac accaaagaca atcaagtccg atagggttat aacagcacct
    10921 caaaaagtgt tagatattat caaagagcaa ataatagaga atgcgacaaa tggatttgat
    10981 acagatttta ttttcatcaa tactctagga gaaccaataa caaatgccag ggttatttgt
    11041 gcattgaaaa gacatggtca aaaaatcggc atagaaaaaa acataactac acatacattt
    11101 agacattctc acatatccct acttgctgag ctgggcattc ccttgactgc catcatggac
    11161 agagtagggc atagtgactc aaagaccaca ctagagattt attctcacgt tactcaaaaa
    11221 atggtatcag acatatctag caagttagac aagataaaat tttaaattgt gcccctcgtc
    11281 tgcccctttt tcttatacaa gacaaacaaa aaccccttaa agtgttgatt ttaaagggtt
    11341 tttaaagtgc acgaaaaaag agcacacaat tcaaatcgct tagggctgct ggattcctcc
    11401 cctgacccgc ttcacgcaga actgttgctc cactatttat tatatcacat tcctattcat
    11461 tttaaaagca aaattatttt ttccgtctat ttctaaaaaa atcctgcatg atagctgcgc
    11521 attcatcttc caaaattcct atttcaacct ccacacgatg attgagacgc tcatctgtca
    11581 agatatcgta caaactccca gcagcgccaa atttctggtt tttagcccca tagaccacat
    11641 ttggaatgcg ggcaagccca atcgccccac tacacatgac acaaggttca atggtcacaa
    11701 aaagtgtgca atccagcaag cgccagctct cctcactcaa gttcgcatcc tctatagcca
    11761 taatttccgc atgcataacc gctcgctgta attcctcacg cgcattatgc ccacgaccaa
    11821 tgatttcccc atctttgaca atcacacaac caattggaat ttcatcgtgt tcaagagcaa
    11881 tctcagcctc tctcaaagcc tccctcataa agacttcttt ttcttcaagc gtataataca
    11941 tctcttttct cttttcctac ttatcgattt tattattata tcatgaatcc caacacaaaa
    12001 gaagccaccg aatgcggtga ctctataggg agattattat gaaaaagaaa agtttaggat
    12061 atttgttaca acaagttagg aggtcttctt gtaactgtct atagtatacc cgacctatct
    12121 taaacaaatc ttaaaaatct cttaggacca aacactttct aaaatatttg tttgttcacg
    12181 accaggacct actgagaaag tagaaatacg aacgccaacc aattcactca cacgacgaac
    12241 atagttacgc gcattctcag gaagatcttc caaattgcga actccggtaa tatcttctga
    12301 ccaacctggc aactcttcat agataggctt gcaacgtttc aattgctcaa gactagctgg
    12361 atagtagtca atacgttgac cgtcaagatc ataggccaca cagattttca cagtatccaa
    12421 accgctcaaa acatcaatag agttcaaaga aaggttagta ataccagaaa cacgacggct
    12481 atgacgcatc acaactgagt caaaccaacc tacacgacgt ggacgaccag ttgttgtacc
    12541 atactcatga cccacttcac ggatacgttc tcccacttca tcaaacaact cagttgggaa
    12601 aggaccatct cctacacgac tcgtataagc tttacataca cctacaacct tgtcaatctt
    12661 gcttggaccg acaccagaac caattgtcac accaccagct acagggtttg atgacgtaac
    12721 aaatggataa gtaccttggt cgatatctag cataacacct tgtgcacctt caaaaagcac
    12781 acgtttgcca ttatcaagcg catcattcaa gataacagat gtatctatca cgtatttctt
    12841 gatttgttga ccatattcgt aatattcttc aaaaatatca tcgaaaacaa tcgctttact
    12901 gtcatacaat ttttcaaaaa gacgattctt ttcagcaagg ttacgttcta aacgctcacg
    12961 gaaaatatct ttatctaaaa gatctgcaat acgaattcca acacgagcag ccttgtccat
    13021 ataagctgga ccaattccct taattgtcgt accaatctta ttgtcgccct tagcttcttc
    13081 ttgcaagcga tccaactcga tatgataagg caaaataaca tgcgcacgat cagaaatacg
    13141 caagttatca gttgttacac cttcctcatg aagatagctc aactctttta caagagattt
    13201 aggatttaca accataccat tcccaatgac agatattttt tcagggaaga aaatcccaga
    13261 tggaatcaag tgcaacttaa atttcttacc gtcaatcaca atcgtgtgac cagcattatc
    13321 accaccttgg taacgtgcaa tcacttctgc attcgctgaa aggaagtctg taatcttccc
    13381 tttaccttca tcaccccatt gggtacctac aacaacaact gaagtcataa ttttgtctga
    13441 gccctcaggc tcttcctttc tcacatacat ggcaggactc tcacctgcaa ttatatctta
    13501 caatttatta taataaaaaa tcgccttttt atcaagaaga aacaatagaa agatttgcta
    13561 tttccaacta ttaaaaaatg atttagaaaa attactagct atttactatt atctttacat
    13621 aaaagagtaa attagttcgg aaatttacta aaattacctc aacaagaaat aaaaccccga
    13681 ttcattacca atttttcaag atacaaacga taagcaacac gataatggta aacgataaaa
    13741 tccctatgac aaccaatgcc atatctcact aaataataaa ttaaaaattt aaaatgaaca
    13801 tgttcccagt caaaattatc accaaatgta ggaccatact cttcttcaat actatcatag
    13861 aaattagcca tctgctcata aattttttgt aacataatca aatactcctt ttctttttta
    13921 taaacttatt ctaacaaaaa attttacaca ttcactatca attcctgaat tgttaaaata
    13981 ccagcctcaa caagataaaa ataggaaaag ttgacaaaat agaaacaaat ttgctaccta
    14041 aatttcaaac aattaaagtt ctaaaaagac cgccttaaaa cttttgggta aatcctatta
    14101 gaactataat atttttatat aggtattatg gttctgattt tctagtataa aaaaattcaa
    14161 actagaattc ttcttagaaa aggccgtttt aagtgctatc attgctcaaa aatggagctc
    14221 gctgagactt ctatcgtcaa gaagaatcat caaattcctt gtatcatcaa ccaaaagatt
    14281 gctcaaaagt taattgaaaa gatttctatg accgacattg atcatcagtt gtctatttca
    14341 acttcaactg tcattcgcaa gatcaataat tttcactttg agcatgattt ttcgcgtctt
    14401 cctgagatta tgtcctagga cgttgaaaca gtccggggag tgactgtttc aatcgggaga
    14461 tggagatgag ctttattgct caggactttg ataaactcaa tatcataact gttcttgaga
    14521 gcagaacaca agccatcatc cgaaatccca tgaatacaag gctatcaagc gctactggaa
    14581 gctcattcaa caagatagtc agaaactgag tgataaacga ttttatcgcc ctacttttcg
    14641 tatgcacttg actaataaag aaattctaga taaacttttg agctattccg aagacttgaa
    14701 acaccactat aatctctatc agctcttgct tttccacctc tgtttcagac tttgtttaaa
    14761 accttcctca aagataaaga gaaaatcgtc aacgcccttc aattacctta ttccaacgcc
    14821 aaactggaag ccaccaataa tctcatcaaa cttatcaagc acaatgcctt tggttttagg
    14881 aactttgaaa acttcaaaaa agaaaggacg aaatttgtcc tttctagatc ttcgctttct
    14941 tcaacccact acagttgaca aagaaccgaa aaatgtctcg cctctaatat agtcaattga
    15001 aacaagaaca agagaaaaga gcctcgtaaa aggtattgca acttggtaat acctttttga
    15061 ggttcttttt gatataagcc catgttttct caataggatt gtactcaggt gagtagggag
    15121 gaagaggtaa aagtttatgc ccaaactcct cgcataa
//
LOCUS       DBERYS010000043        14920 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.043, whole genome shotgun sequence.
ACCESSION   DBERYS010000043 DBERYS010000000
VERSION     DBERYS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.043"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..166
                     /locus_tag="ACL8LA_001620"
     CDS             <1..166
                     /locus_tag="ACL8LA_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1813355.1"
                     /translation="QLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKK
                     ERTKFVLSRA"
     gene            complement(265..1887)
                     /gene="groL"
                     /locus_tag="ACL8LA_001621"
     CDS             complement(265..1887)
                     /gene="groL"
                     /locus_tag="ACL8LA_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGS1813356.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1903..2187)
                     /gene="groES"
                     /locus_tag="ACL8LA_001622"
     CDS             complement(1903..2187)
                     /gene="groES"
                     /locus_tag="ACL8LA_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGS1813357.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2343..2738)
                     /locus_tag="ACL8LA_001623"
     CDS             complement(2343..2738)
                     /locus_tag="ACL8LA_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGS1813358.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2816..3577)
                     /locus_tag="ACL8LA_001624"
     CDS             complement(2816..3577)
                     /locus_tag="ACL8LA_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1813359.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3619..4245)
                     /gene="ytpR"
                     /locus_tag="ACL8LA_001625"
     CDS             complement(3619..4245)
                     /gene="ytpR"
                     /locus_tag="ACL8LA_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGS1813360.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(4261..4578)
                     /locus_tag="ACL8LA_001626"
     CDS             complement(4261..4578)
                     /locus_tag="ACL8LA_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGS1813361.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4575..4874)
                     /locus_tag="ACL8LA_001627"
     CDS             complement(4575..4874)
                     /locus_tag="ACL8LA_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGS1813362.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5072..5152)
                     /locus_tag="ACL8LA_001628"
     CDS             complement(5072..5152)
                     /locus_tag="ACL8LA_001628"
                     /inference="COORDINATES: protein motif:HMM:NF039231.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813363.1"
                     /translation="MASSGKMLRNTYTPDGYYVGNLGAWQ"
     gene            complement(5159..5302)
                     /locus_tag="ACL8LA_001629"
     CDS             complement(5159..5302)
                     /locus_tag="ACL8LA_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HGS1813364.1"
                     /translation="MILDSFFAFNCSGTMKVSTWVYDKGEWYYVSSSGSMIANDWVKD
                     NGK"
     gene            complement(5511..6185)
                     /locus_tag="ACL8LA_001630"
     CDS             complement(5511..6185)
                     /locus_tag="ACL8LA_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813365.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6191..6637)
                     /locus_tag="ACL8LA_001631"
     CDS             complement(6191..6637)
                     /locus_tag="ACL8LA_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1813366.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPCYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6751..7254)
                     /locus_tag="ACL8LA_001632"
     CDS             complement(6751..7254)
                     /locus_tag="ACL8LA_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS1813367.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7251..7613)
                     /locus_tag="ACL8LA_001633"
     CDS             complement(7251..7613)
                     /locus_tag="ACL8LA_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HGS1813368.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7679..7828)
                     /locus_tag="ACL8LA_001634"
     CDS             complement(7679..7828)
                     /locus_tag="ACL8LA_001634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813369.1"
                     /translation="MSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNSQFTE
                     DEAEE"
     gene            complement(7856..9580)
                     /locus_tag="ACL8LA_001635"
                     /pseudo
     CDS             complement(7856..9580)
                     /locus_tag="ACL8LA_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9555..10026)
                     /locus_tag="ACL8LA_001636"
                     /pseudo
     CDS             complement(9555..10026)
                     /locus_tag="ACL8LA_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            complement(10150..10866)
                     /locus_tag="ACL8LA_001637"
     CDS             complement(10150..10866)
                     /locus_tag="ACL8LA_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1813370.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11747..13162)
                     /gene="ply"
                     /locus_tag="ACL8LA_001638"
     CDS             complement(11747..13162)
                     /gene="ply"
                     /locus_tag="ACL8LA_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGS1813371.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13173..13583)
                     /locus_tag="ACL8LA_001639"
     CDS             complement(13173..13583)
                     /locus_tag="ACL8LA_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813372.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTGIPTSYLARRRRN
                     VSEELYEEILDYLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(13576..14184)
                     /locus_tag="ACL8LA_001640"
     CDS             complement(13576..14184)
                     /locus_tag="ACL8LA_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813373.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14197..14655)
                     /locus_tag="ACL8LA_001641"
     CDS             complement(14197..14655)
                     /locus_tag="ACL8LA_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGS1813374.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            14780..14890
                     /locus_tag="ACL8LA_001642"
                     /pseudo
     CDS             14780..14890
                     /locus_tag="ACL8LA_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         4351 a         3451 c         2694 g         4424 t
ORIGIN      
        1 tcaactacac tattctaatg ccaaactgga agcgaccaat aatctcatca aacttatcaa
       61 gcgcaatgcc tttggttttc ggaactttga aaacttcaaa aaacggattt ttatcgccct
      121 gaatatcaaa aaagaaagga cgaaatttgt cctttctcga gcttagctga cttcaaccca
      181 ctacagttga caaagagcct ataaaaagaa gggttagtca ttccctcctt ttgtgttttt
      241 tataagttgt tttctataga aagcttacat catcccgccc atcatacttg gatccattgc
      301 tggagctggg gctactggtt ctggtttatt ggctacgact gcttctgttg tcaaaatcaa
      361 gctggctaca gatgctgcat tttgtagggc tgaacgactc actttaactg gatcaatgat
      421 accttgatca atcatgttaa cccactcgcc agttgctgcg ttaaatccta taccaagctc
      481 agcatttttc aaacgatcga taacgataga tccttcaaat cctgcattgt gagcaatttg
      541 acgaacgggt tcttccaaag cacggagaac aatattacgt cctgttgctt catctcctgt
      601 caattccaag gtagcaacag ctggaatcac attggcaaga gctgttccac cgcctgcaac
      661 aataccttct tcaacagctg cacgagtagc gttgagggca tcttcaatgc ggagtttcat
      721 ttctttcaac tcagtttcag ttgcggctcc gaccttaata accgctacac cacctgacaa
      781 tttggccaag cgttcttgca atttttcacg gtcaaattca gaagttgtag tttcgatttg
      841 agacttgata accgcaacac ggtgagaaat cgcttcagga tttcctgcac cttctacaat
      901 aaccgtgcta tctttgtcca cggtcactct cgctgcttga ccaagagctt caattgtcgc
      961 atctttcaac tcaagaccaa ggtcttctgt gataactgtt ccgcctgtta agatggcgat
     1021 atcttcaagc atggctttgc gacggtcacc aaaaccaggt gccttgactg ctactacgtt
     1081 gaaggttcca cgaatcttgt tcaaaacaag agttggaaga gcctcgccat ccacatcatc
     1141 cgcaataatc aagagtggac gattgctttg gagaatgctt tccaaaagtg gcaagatctc
     1201 ttggatattg gaaattttct tgtctgtaat caaaatgtac ggattttcaa ggtcagccac
     1261 cattttttcg ctatctgtca ccatgtactg tgaaaggtaa ccacggtcaa actgcattcc
     1321 ttctacgact tcaagctctg tttccatacc acgtgactct tcgatggtga tgacaccgtc
     1381 tttgccaact ttttccattg cttcagagat gtactcacca actttttcag aacgagaaga
     1441 tacggctgca acttgagcga tagcttcttt attggcaaca gggatggcgt tgtttttcaa
     1501 agcttcaact gctgcggcaa ctgctgtttc aatcccacga cgaataccga ttggatttgc
     1561 acctgctgtg acgtttttga ttccttcacg gacgatagct tgggtcaaga ctgttgcagt
     1621 cgtagtcccg tcacctgcga tatcattggt tttagaagct acttctgata ccaatttggc
     1681 acccatattt tcaaaatggt cttctaattc aatttctttg gcaatagtca caccgtcatt
     1741 ggtaatcaat ggtgagccaa atgatttttc aagaacgaca ttgcgacctt ttggtcccaa
     1801 ggttacttta acagtatctg caaggatatc gacaccacgg accatagctg aacgggcatc
     1861 tgatgaaaat ttaatttctt ttgacatact tactttctcc ttctattctt caatgattgc
     1921 caagatgtta gcttcgccta cgatgatata cttttcatcg ccatctttga catcaagacc
     1981 tgcgtgggct tcaactaaga cacggtctcc agttttaaca cttggagcaa ccaagtcacc
     2041 gttcaaggta cgaacacctt gtccagtagc cacaacttgg gctgttttgg ttttttcttg
     2101 ggctgaacct gcgagaacaa agcctccaac agtttgttct ttttcttcta cttttaagac
     2161 caaacggtcc cctaatggtt tcaacatctg atttcctcca taatgagata gatattatta
     2221 gcactcttta tacctgagtg ctaattcata gttctattgt atcacttagt cagaaataat
     2281 caagaaaaaa gtctgacttt ctcaagataa aaagcctgag accaactcag actttttaat
     2341 tcttaaaatg gcaattcttc ctcttccaag accaaatctg ccaaatcttg gcctgcatta
     2401 ttttcacgca tagcacgttg ggcacgactt tccaagagtt ggaatcctgt cacaaggact
     2461 tcggttacat aattcatctg accatttttc tcaaagcgac gggtacgcaa ttctccatca
     2521 acggaaatga gactaccttt ggttgcgtag cttgccaaag tttctgctag tctgccccat
     2581 aggaccatat tgacaaaatc aacttcacgt tcaccgtttt ggtctttgta acgacggttg
     2641 acagcgatag ttgctcgcgc tactgacttg tcattgttgg ttttgtgcaa ttctggtgta
     2701 gacgttaaac gtccaatcaa gataacttta ttatacatat tttcttcctc ctacttatct
     2761 attcgtagga aatcaaaaaa agttacagaa atttgtaact tttcgagaaa attttttatt
     2821 ttttatgaac catgaaacct gtcgcctgtt gattggccat aatggtcata tctgtaatct
     2881 gaacacgacg aggttgacta gtcacataga ctactgtatc tgcaatatcc tgagcttgca
     2941 aagcttctat tccttggtaa acggacgcag ctcgttcttt atcaccatga aaacgcactg
     3001 tagaaaaatc tgtttcgaca attccaggct gaatggttgt caccttgata tccgttgcga
     3061 tggtatcaat tcgcagtcca tccgaaaagg tcttaaccgc agccttggta gctgagtaaa
     3121 cagcggcacc agcataggcg tagattcctg cggttgaccc catattgata atatgacctt
     3181 gattgacttt tatcattgct ggcaagaaac agcgagtaac cgccatcaga cctttaacat
     3241 tggtatccaa catggtcaac atatccaact cctcatagtc ttgataggga gccaagtcaa
     3301 gagccagtcc ggcgttgttg accaagatat caatcctccc tatcgtttct agaatatcag
     3361 agcaaacagt cttcaccatg accatatccg tcacatctag tggaaaagtc cagacggttt
     3421 gatttggaaa gcttactgca aactccgact tgaggatttc taatctgtct atccgtcgtc
     3481 ctgttagaac gacatcctca ccctgctcca gataagcacg cgcaatcgct tcaccgattc
     3541 ctgaggttgc tcccgtaatc acaacatttt ttgccatctt atttccttct agctggtcta
     3601 tcagatatta acaacttctt aggcagtcca gtgtttagct gagtcaaatg gtgttccgac
     3661 aacttggtct tctgataatt caagcacccc acgtttttgt ggagcatttg gcagatgcaa
     3721 ttcacgagga ctgcacatca taccaaaact cttttcacca cgaagttcac ctgggaaaat
     3781 gagattccct tttggcatca tagctccagg aagcgcgaca atggttttca accccacacg
     3841 cgcattggga gctcctgcaa cgatttgtac agtcttatca cttgcgactg caacttggca
     3901 gatgttgagg tggtcactat ctggatgggc taccatctca acaatttcac cgacaacaaa
     3961 cttaggttcc ttatcattaa caatttcttc tgtaaaacct tccgcctgca actcttggtt
     4021 caaacgagcg acttgctcat ctgtcaaaaa gacttgaccg cgctctgcag tttcaaataa
     4081 acttgaaact tcgaaaatat tccaagccac tgtttcccca ttttctttga ggaaaacacg
     4141 ggctaccttg cctttgcgtt ccacattcag tttggcatct ccgctatttt tcacgatgac
     4201 cataaggaca tcaccgacat gttctttgtt atacgtaaaa atcattgttt cctttttctc
     4261 ctatttcaat cctgctaaaa aatcgttaat ttgctcctta cttttacggt cgcgattgac
     4321 aaaacgaccg atttccttgt ccttttctag aacaacaagg ctaggaattc cgtaaacatc
     4381 ccagagtttg gccaaatcca tatactgatc tcggtccatt cgaataaagg tgaactctgg
     4441 attggtctct tcaatctctg gtaaggcagg ataaatataa cgacaatcgc cacaccagtc
     4501 tgccacaaaa aggaagacct tcttgcccgc tttttccact aaagatgcta attcttctaa
     4561 acttgctggc tgtatcataa gacttcctcc tcatagacta ggtcttcatt ttcatagaca
     4621 aaggtataat gacggccatc ctcaaaaatg acgccaccaa ccaagctctc cagactgctt
     4681 tcgtaaactt gaacataaag ggtcgcaatt tcccccatgt cggaaaaatg gtctcgcaca
     4741 atctctgtca actcttcctg agtcttcatg agcttacggt catctgcaac ttttttcgta
     4801 gcaagggcaa ggcttccgat acctagcaga gccaagcctg ccatccacat ttttttagct
     4861 ttcataccat tcattttaac acaaaaaagg cttcaggaca aatgaggaag cagcagaaaa
     4921 gcaagtaaaa agcctcttcc tttaaggaaa aggacttctt atactaagag caaactagaa
     4981 agctagccgc aggctgctca aaacacggtt ttgaggttgt agataaggcg acgttgacgt
     5041 ggtttgaaga gattttcaaa gagtattatt cttattgcca ggcacctaag ttgccaacgt
     5101 agtaaccatc aggtgtgtag gtattgcgaa gcatcttacc tgatgaagcc agataatact
     5161 acttgccatt gtctttgacc caatcattcg caatcatgga accagaagaa cttacataat
     5221 accattctcc cttgtcataa acccaagtac tgactttcat ggttcctgag caattaaagg
     5281 caaaaaaact gtccaatatc attcgttttt taaaagcatt cgacactaca tcatctacgc
     5341 tgtagtacat agatacatca aacgtaaaag ccaaaatatc gaaaaatata caacttttaa
     5401 aggcagcatt tcatttttta aagtctaaca accatagaac tgaaatcaga gcaaggcatc
     5461 aatttcctac tctatagtac gcctattttg atggttgaag tgaagatttt ttatttaacg
     5521 tagacaattt ccaacttccc aaaagccaca tctccacgga taatcaaggt tttgctggtt
     5581 ggggctacag gagcgtcagc cttagctgca ccaaaggagg tttctacttt caaatctaca
     5641 cgccagtgtt gtggaacata gacggttgca tttccgaagg ctacttcaat atttaaagtt
     5701 gcaaaatcac ctagcatctc tgcattatca tagtagattt tagcatcccc aaaagcgact
     5761 tccacttggt catctacgag atcttgatct tgcttataga aggtcccgct accaaaagcg
     5821 acttccttat ccgtgacgac tgtttttttc ccattgtacc accatttttt ttcattccag
     5881 aacttacttg aatgcgtcag ataaccaaca ccaagtacca ccaagatgct agcccaaata
     5941 agagaatgat tggtaactgg taacaagtca taagcgtaat ttgcaatgat gagcgccagt
     6001 aaacctgtaa aaactgccga agtgagatga cgtctaagga tggactcaat ggacttataa
     6061 gcaaaaaaaa caatacctag taaaggccat attttaccat ccaaagaagg aattccaaaa
     6121 ttcccttgca gcaagatcca agctgctaaa accaataaaa caataccaaa tgctttcttt
     6181 ttcatgtttt ttacctcatg tttcttagat tttcttttag gagggattgg taatgccgtg
     6241 agacatgaac ctccttgtgc gtctgataaa aggaaatggt gcccgttcct gaaaaggact
     6301 tgtccactga gtaaatctga cggatgtttg cgatcgttga cttggaaact cgattaaaat
     6361 agcaaggcaa gatagactcc aactcataga gcttgaggcg aacctcataa gcttccttct
     6421 ggttatgagc gtagatcttg ctcccttctg tttcaaagaa gagaatttct gacaggtcta
     6481 gataatattc acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac tcttgcaaga
     6541 gccgttgcaa gtctgcaatt tcatctgtca aagtgggggt cttgatgaca atttcagtct
     6601 cctctaaatt gccgtcaatc tcaattcgta acttcatcac atctcctttc tgactctact
     6661 atatcaaagc taagttagga atacaagggc aaaaaagcaa gtggtcaatt tgaagcagta
     6721 agtggttgta ggactagctt aacttacatg tcagaatagg aagaaaagga atccccacac
     6781 caaaccacaa gcatagccaa ccaagacatc cttgggataa tgaacacccc ctaaaaccct
     6841 caccaacccc agcaaagctg aaaagatcaa gcatactaag ccaataggta gactagcatg
     6901 cagacaggcc atggagatga tagttgccga aaagacatgg cgactgggca tcgaccgtcc
     6961 cgaactatcc ttatcaagaa ggggacagat atcccaagtt tcataaggtc tcgggtgatt
     7021 gattttctta cgaaacaggg acaaggtcac aaaacctgat gcaggaataa gcacataaac
     7081 cccaacttgc tgaccaagtc ctagttgcag gtaagtggtg actagcaaga tcagatagac
     7141 caggggcatg gcgactgtca tcaaacgatt gaaactgcgt aataggaata aaagaattgg
     7201 acgactagtt agtctggagc tgatatttcg ataccattct tgataatttt tcatttatgc
     7261 ttctcatact tttctagaac ttgttgagat gttttcccct tggcaagcca atccactagc
     7321 ttgtccaagc gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat acggacaccg
     7381 caaatcttcc ctgtaattgc aattcgttca gggacataat atggtgcttg tctaaaaaaa
     7441 tctccgtaag taacatccct atccagacaa gcaagaacat cactcacttc ataccctgtc
     7501 aaccaactgg tcacttggtg aactgactct gcctttccac cctttcgctc aacttttgca
     7561 atcaaggcta agtagacagc agcaaatttc atattgtata atttctgact catacttctc
     7621 tctttctatg tgtactagta taaataagaa aaagaaggcc gtcaagcctt cttttgattt
     7681 attcttctgc ttcatcttct gtaaattgac tattgtacaa gtcagcgtag aagccacctt
     7741 gcgccatcag ttcctcatag ttgccttgct cgatgatatt tccatctttc atgaccaaga
     7801 tcaagtctgc atttcggatg gttgacaagc ggtgggcaat gacaaaggat gtgcgtcatt
     7861 ccatcaaacg gtccatggct ttttggatca attcctctgt ccgtgtgtca acagaagaag
     7921 tcgcctcatc caaaatcaaa agcggtgcat ccttaagaag ggcacgagca atagtcaata
     7981 gttgtttttg tcctacagac aaggtcacgg tgtcatccaa gatggtatca tagccatctg
     8041 gcaaggtcat aataaagtgg tgaattccca cagccttact agcttccatc attcgttcat
     8101 cactaatccc tatttgatta tagatgagat tgtctcgaat agttccttca aagagccagg
     8161 tatcctgcaa gaccattgaa aaggcatcat gcacttctga acgcgtcata gccttggtat
     8221 ccacaccatc aatgtgaata cttcccttat caatctcata gaatttcatc aaaagattga
     8281 caatggttgt cttaccagtc ccagtcggcc caacaatggc aaccttttga ccagcatgag
     8341 ctgtcgcaga gaagtcatag tcttgaacat tgacaccgtc caccagaatt tctcctgctg
     8401 acacgtcgta gaaacgtgga atcagattga ccagagttga tttaccagaa cctgttgacc
     8461 caataaaggc cactgtttga ccagtttctg ctttaaagct aacatgttca ataactgcct
     8521 ccgaatttgc cgcatagcgg aaggtcacat ccttaaactc gacctgacct ttgaagtttt
     8581 catcagtcag ctgcacttga acagggtttt ggatagaaga atgcaaatct aaaacttgat
     8641 taatccgctt agcagagacc atagttcggg gaagaacgat gaagagtgct cccatgagaa
     8701 ggaagcccat gacaacctac atggcataag acatgaaaac aatcatgtca ctaaagagag
     8761 gcagacgcgc tatcgaagca gcgtcgttaa tcacataggc cccaatccag taaatcgcta
     8821 cactcaaacc acttgaaatc cccatcatga taggattcaa aatagccata agacggttga
     8881 caaacaaatt caaacgggtc aattcatcat ttactgctgc aaatttttca ttttgataat
     8941 cctctgcatt gtaggcacga acgacacgaa tacctgttaa actctcacga gtgatactgt
     9001 tcagtttatc tgtcaacccc tgaatcaagg actgttttgg aaaggctagc gtcatcaaaa
     9061 cggtcgtcat caggacgttg acaatcacta ccacaagtac ggcccagagt cagtattctg
     9121 aatgacctaa aatcttccca atagcccaga tagccataat tggaccacgc gttaccactt
     9181 gcaagcccat agtaatcaac atttgaactt gagtaatgtc attggtagta cgcgtcaaga
     9241 ggctaggaat tgaaaatttc ttaatctctg tctgcgagta atccaaaact cggttaaaaa
     9301 tatcacttct cagcctacta gtataagaag ccgccactcg ggatgcaaaa aatccaactg
     9361 caactacgga caagaaggca agaaagagca ttcccatcat catgcttgcc ggctgccaca
     9421 actcatctaa attagtttct tgactaccta gcaaatccgt aattttcgag atataggtcg
     9481 gcacttccaa ctctagatag accgaaaagc aagtaaagaa aatggctagt aaaatcatcc
     9541 cccattcttt tctactaatt cttttggtta atttctttat tctctcctcc tattcccttg
     9601 atattttgcc tgtagttgac cgagaacttt ctcaaaaatc agtaattcat cttcatcaat
     9661 gtcttccatc aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt gcatctgaga
     9721 acgtgctttg tccgtcagac gaacaaactt agcccgctta tcaacaggac tcgcctccaa
     9781 ttccaccaaa ccattttgca ctatacgctt aaccagatta ctagcaacag acttggtaat
     9841 attgagttcc tgctcgatat ctttaatcaa gaccaagtct tggtttttct agcgattatc
     9901 caaaaaacgc acaacctgac cttgcggccc acccataaat tcaatgccgc aacgtttggc
     9961 ttccttttgc accatcaggt gaatttgatg accaaaacgc ttaaagacta acatcggttt
    10021 atccataatc tcccccttct aaataaaaat agttctctgg agaataatta aatttctatg
    10081 agaactattt tcttgattaa aaaaatccca agtgattttc tcacttagga tcatgttcta
    10141 taggttaaat taaaacccat ctacgttcgt ataaatcttt tggacgtctt cgtcgtcttc
    10201 aagaacgctg taaagttttt caaaggtttc aaggtcttcg cctgacaatt ccacttctga
    10261 ctgaggaatc atttccaatt cagtcacttg gaattcttca ataccagact cacggagagc
    10321 aacgatagcc ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac cttcttgtgc
    10381 ttctacgtca tccacatcca catccgcttc gagcaattgc tcaaagactg cgtctgcatc
    10441 ttcacctcca aatacaataa cacctttgtt gtcaaagagg taagaaacag aacctgaagc
    10501 gcccatgttt ccgccgtttt taccaaaggc tgcacggaca ttggctgctg tacggttgac
    10561 gttagaagtc aaagtatcca caattagcat agagccattt ggcccaaaac cttcgtaacg
    10621 tccttctgta aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt tatcgataat
    10681 gtgttttggc acttgggctt gtttagcacg gtcgataacg aatttcaaag ctgagtttga
    10741 ttctggatct ggatcacctt ttttagctgc tacatagatt tctacaccaa attttgcata
    10801 taccttagag ttagctccat ctttagccgt tttcttggct acgatattgg cccatttacg
    10861 tcccattagg aatctccttt tttcacattt taatctttat tattataaca caagtttttt
    10921 tgattttcac tagaggaaat ggattttatt agcaaatcaa gctaggatag cactttacct
    10981 gctaagatgg tcttaccttt ctatctttat caacagacac tcatccacat tcaaaaaaca
    11041 aactagacca ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt cagctcaaat
    11101 tactgtttga agtttgtaga tataagcgac aaaaacaatc atactgcacc ttttgttgac
    11161 agtctactcc agacatatca tagttcaagt aaatactttg aaattcaaca gttcttatag
    11221 gcgctattgt attctaagaa atcaatagaa gagtttctaa gcaaacctct aatactcaat
    11281 aaaaatcaaa gagcaaacca gaaagctagc cgcaggttgc tcaaaacact gttttgaggt
    11341 tgcggatggg gctgacatgg tttgaagaga ttttcgaaga gtataattta cgtgttccca
    11401 agatggagaa gttagactag tacactggca cttctaaacc attgctagca attgatttgt
    11461 tcatatttaa tttcattttt tccataaatg ggtattagat ataaacagca aaatatttcc
    11521 gatacgtgtc gttcttgaat ttccaatcat ctaaaacaag taaaggataa tcaatcccct
    11581 gtatatcaag gaattggcta ccctttttac ttttttacac attctgtttg atagattcat
    11641 tttaacatca cgagcatact ccaatggaaa tcgctaggca agagataaac tttcagatat
    11701 ccgcagagag atcatcgcct ctttttgtcg caagcattct cctctcctag tcattttcta
    11761 ccttatcttc tacctgagga tagagagttg ttccccaaat agaaatcgtc cgcttacgca
    11821 ctagtggcaa atcggttttt tcataaaccg tacgccacca ttcccaggca agcccggtac
    11881 actctctaat tttgacagag agattacgaa cattcccttt taaaggaata ctagtggtaa
    11941 agtgagccgt taaatcctgc ccatttctgt cccaagcctt aggagtcaag acttccttac
    12001 cttgatgatc ataggataat tcattccaag taatataata ttgggcaaca taggcaccac
    12061 tatgatccag cagtaaatct ccgtttctgt aagctgtaac cttagtctca acatagtctg
    12121 tactattttg aaaggtcgca actacattgt cacgtaaaaa agaagttgta taggaaatcg
    12181 gcaagcctgg atgatctgct gtaaagcgac tgccttcttg aatcaagtcc tctaccatat
    12241 ccaccttgcc tgttacaact cgggcacccg aacttgggtc gccccctaaa ataaccgcct
    12301 tcacttctgt attgtccaaa atctgtttcc actctgtctg aggagctacc ttgactcctt
    12361 ttatcaaagc ttcaaaagca gcctctactt catcactctt actcgtggtt tccaacttga
    12421 gatagacttg gcgcccataa gcaacactcg aaatatagac caaaggacgc tctgcagaaa
    12481 ttcctctctg ttttaaatcc tctaccgtta cagtatcttg aaacacatct cctggatttt
    12541 taacagcatc tacgctgact gtataataaa tctgcttaaa attaacaatc tgaatctgct
    12601 tttcgcccga atggacagag ttaaaatcaa tatcaagaga attccctgtc ttttcaaagt
    12661 cagaaccaaa cttgaccttg agttgttcca tgctgtgagc cgtgattttt tcatactgca
    12721 ttctagctgg gacattattg acctgaccat aatcttgatg ccacttagcc aacaaatcgt
    12781 ttaccgctcc gcgaacactt gaattgctgg ggtcttccac ttggagaaag ctatcgctac
    12841 ttgccaaacc aggcaaatca atactataag tcatcggagc acgatcgacc gcaagaagag
    12901 tgggattatt ctctaacaag gtctcatcca ctacgagaag tgctccagga tagaggcgac
    12961 tgtcgttggt agctgttaca gaaatatcac ttgtatttgt cgacaagctc cgcttctttc
    13021 tttcgataac aacaaactca tcgggtagct gattaccctc tttgatgaaa cgattttcaa
    13081 tactttctcc ctgatgggtc aagagtttct ttttatcgta attcatagct agtataaagt
    13141 catttactgc tttatttgcc atcttctacc tcctaataag ttcctggatt gagttgcata
    13201 aactcagact tgttcagcga aatcagccgt ggttggacta agtaatccaa aatttcctcg
    13261 tacaattctt ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg aatgcccgta
    13321 ttatccaaca taaatacctt atctaagtca atcaaggttg gtcttgtaaa aggattacga
    13381 gctagatccg gctcttctat cataaagttc ttgaccaaac gtctggtcaa gagagctggt
    13441 ttgaaggtct gatttttaac caactctttg tttttagtca tgctgttgtc aatacagata
    13501 tacatatgat tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa ataaagagct
    13561 acaacatctc ctctcttaat caagcaagag cacccccttt tctcctaatg taacatagac
    13621 aggattgacc aagtcttctg attgactcag aatttccaaa gtttgagttt ggcgcgctgt
    13681 caatttagta gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa ccttgacaat
    13741 ataatccttc tccaaagctg actggtaaat ccacatcaga tgttgtctgt cctgagaact
    13801 caagagagaa ggattttcaa gcctcccgat agtctgataa aaatcaaaaa caggagctaa
    13861 ctcctgccaa tctgattggc tagttgtcaa ggctagaaaa agggctttgc gagctgatac
    13921 ttcttggtta gccttgagag ttactttccc ctccaagttt tttagaaatc gggaaactcc
    13981 agaaagcaaa tttttctcta actgcgagaa ataaaaacct ttcgttccca gacataagtc
    14041 tttcatgtcg ctttctctag caaataagag ctcaaacatt tgatagtaaa agaaaaatat
    14101 ctggcactgg gtcgcgctca tcttttcctt atcggcttct ttttttaacc agagcaaggg
    14161 cgacaggtaa ctggattgag acatttcctc tacctcctac tcttttttaa ctggagcatc
    14221 tgcactagct gccacttctt ttgactggat actttcccac tggttaatct cctctgagat
    14281 aagaccttcg catgtcttga caaatagggc aaaagccttg gtctttcctg catatttctc
    14341 cgtttggcat tgatagagga atttttcttt ctccaggagt tgcgcagttt tttggtaaga
    14401 aatccaattt tcctttgcat tatacaaatt gataatcccc tcacacagca agccgagact
    14461 ggataaggca accgaaatca aacggtagcg atcacctggc ataggaatag cacaaaagac
    14521 agctatgagg aaacctgcca cgatttctgt tatttttaat accttatagc gcctacgatg
    14581 ttgaacgctt ttctttaaaa aatgagctat ctgtacgtct aatcgctctg tcaggtacat
    14641 ttcttctggc gtcatattcg taactccttt catttacttt gataaccata gtataacata
    14701 tttgaatatc aaagtatagt aaaaactgga ctataactta ttagacttcc tgcgaaacaa
    14761 aatatggtat agtagttcta tgaattatga agcaagtaaa caactaactg atgcacgatt
    14821 taagcgtctt gttagtgttc agcgtaccac ttttaaagag atgttagctg tattaaaaac
    14881 agcttatcaa aaaagtcgga caagctggta aaatcttggc
//
LOCUS       DBERYS010000044        13882 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.044, whole genome shotgun sequence.
ACCESSION   DBERYS010000044 DBERYS010000000
VERSION     DBERYS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.044"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..209)
                     /locus_tag="ACL8LA_001643"
                     /pseudo
     CDS             complement(1..209)
                     /locus_tag="ACL8LA_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(271..720)
                     /locus_tag="ACL8LA_001644"
     CDS             complement(271..720)
                     /locus_tag="ACL8LA_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS1813375.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(722..1480)
                     /locus_tag="ACL8LA_001645"
     CDS             complement(722..1480)
                     /locus_tag="ACL8LA_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS1813376.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1595..3574)
                     /gene="stkP"
                     /locus_tag="ACL8LA_001646"
     CDS             complement(1595..3574)
                     /gene="stkP"
                     /locus_tag="ACL8LA_001646"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGS1813377.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3571..4311)
                     /locus_tag="ACL8LA_001647"
     CDS             complement(3571..4311)
                     /locus_tag="ACL8LA_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGS1813378.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(4326..5639)
                     /gene="rsmB"
                     /locus_tag="ACL8LA_001648"
     CDS             complement(4326..5639)
                     /gene="rsmB"
                     /locus_tag="ACL8LA_001648"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGS1813379.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5632..6567)
                     /gene="fmt"
                     /locus_tag="ACL8LA_001649"
     CDS             complement(5632..6567)
                     /gene="fmt"
                     /locus_tag="ACL8LA_001649"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGS1813380.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6580..8976)
                     /locus_tag="ACL8LA_001650"
     CDS             complement(6580..8976)
                     /locus_tag="ACL8LA_001650"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGS1813381.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHARDVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9042..9356)
                     /gene="rpoZ"
                     /locus_tag="ACL8LA_001651"
     CDS             complement(9042..9356)
                     /gene="rpoZ"
                     /locus_tag="ACL8LA_001651"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGS1813382.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9381..10007)
                     /gene="gmk"
                     /locus_tag="ACL8LA_001652"
     CDS             complement(9381..10007)
                     /gene="gmk"
                     /locus_tag="ACL8LA_001652"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGS1813383.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10137..11750)
                     /locus_tag="ACL8LA_001653"
     CDS             complement(10137..11750)
                     /locus_tag="ACL8LA_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGS1813384.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11832..12101)
                     /locus_tag="ACL8LA_001654"
                     /pseudo
     CDS             complement(11832..12101)
                     /locus_tag="ACL8LA_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(12233..12991)
                     /locus_tag="ACL8LA_001655"
     CDS             complement(12233..12991)
                     /locus_tag="ACL8LA_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813385.1"
                     /translation="MKFKNYLFDLYPYFPKGFSLDNREDPDVCSKALYDDLCKMFFDD
                     DSKEKLKITSVCNKCQNYGNRDYYTLFIDKDKYLLSSDYIGASIYWAQEAGLNDRIIL
                     DHLSISRTIGGHILFPRGGKLETVNQARGGEKGYYDRFDLTLYAIKEWFVENKNTKIG
                     YAIENYHEWFELFSGDDNCKNGFENFVEFFKLEGFIYEQNKIIDLIKSDLENNQVVFL
                     DKEDILIASTEEEYIRYMKNLNIIILERTKKILL"
     gene            complement(13007..13147)
                     /locus_tag="ACL8LA_001656"
     CDS             complement(13007..13147)
                     /locus_tag="ACL8LA_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813386.1"
                     /translation="MLFYLTKELNSGVKKIGTISTTLVVVAGVSAGVLGGKFDFKFPK
                     KI"
     gene            complement(13101..13604)
                     /locus_tag="ACL8LA_001657"
                     /pseudo
     CDS             complement(13101..13604)
                     /locus_tag="ACL8LA_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370715.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
BASE COUNT         3815 a         3237 c         2586 g         4244 t
ORIGIN      
        1 attcgctaat tgttttttta ggacaattga tttgtacttc cgtcgcatca atcattaccg
       61 tgtcttcaga actgagagga gttcttgaaa tcgtaacacc acctttgaac aagagttact
      121 tcaacccatt ggctccgacg gagtaagttg ctttcgtgga taccaaaatc agccgcaatt
      181 tcttcataag tgcggtattc tcgcacatat agaaagcgtt gttaatttat ttatctcatt
      241 tttcagaaaa ttcttttatt tctgtaaagt ctacgatact cgatgtgttt ttatataatg
      301 atacagtctg agaatcactg ttccgctagc cattccaata gagattacca aagccaacat
      361 gacaaccaag gtcgcacttg ccagtgcttt attatagtcc cctgtcacaa aaaaggcagt
      421 tgttcggtag gagagataac ctggaaccag cggtgccaaa atggccaaga taaagaccac
      481 agcaggtgtc ttataaagaa tacttaaaat ctggctgaca caagaaccaa taatggctgc
      541 aatgaaggta gctacaatga cattggtcgg ttccttgagc aagagataga ttagccagac
      601 agtcatgccc aaaatccctc caggtaagag catagaccgt tgcacattga gtacgattaa
      661 aaaagtgata atggcaagaa aacttgctac tgcttgtaat aaaaaggttg ttagtgtcat
      721 attagttcat caataccaag gcgacagaag ttcctgcccc taaagcgagg gtaatgagca
      781 gggattcaaa catcttactc ataccagagt ttatgtggtt ggtcataata tcacggaccg
      841 cattggtcaa ggcaatacct ggtacaaacg gcatgaccgc accagctata atcaaatctg
      901 ccgttgaagg aaaacctgtg tagcgagccc aaaactgggc aattatccca aagacaaagg
      961 ctccagcaaa ggctgtcaca aagggaattc ggataaattt ttccacatag agggaaaagg
     1021 caaaaccaaa taaggtcgcc actcctgccc caagtgcgtc gtagatattt ccgctaaaca
     1081 taactgaaaa gaaaggagca ctaaaggtcg cagccagagt tacctgcaac ttagtatagg
     1141 gaaggggttt ggcttgcaag gccgtcaatt gcttaaaggc tgtttctaag tcaatctgcc
     1201 ccccaactag ctgacgagaa atctggttca catcgcagac tttttcgatg ttataagaag
     1261 aggaggtcac gcgcttcatg cgcgaaatat tggtattttc aatagagaaa aagatagcgg
     1321 caggcatggc aaggacattg caatccacaa tcccctgcga atgcgcgatt cgaatcatgg
     1381 tatcttctac acgatggatt tctgagccac ttttaaggag aatagtcccc gctagcataa
     1441 tcacatcaat gacggcattt aattcttttg attcttccat gctttcctcc ttttatcaac
     1501 tccctctatt ctatcacaaa tccggactca aaaaaaatct ttgccatgaa atcatgacaa
     1561 agattgatta ctcattttga ttatccatct gcttttaagg agtagctgaa gttgttttag
     1621 gtttgtagat tgaaatcttg actctagtct tatttaggtc taccttttca cctgctctag
     1681 gactttgttc aacaaccatg ccttctacac tacctgcagg cgctgtcgtc acttctacaa
     1741 cttctatatt agcttcctta atcccaacaa tttgaatcaa attgttctta gtaaactcca
     1801 agctggaacc aatgtaactc ggcatggcaa cacttgtaac ttttttagct actgtcaaga
     1861 caatttgagt aggtttactc acatcataag tcgttccggc acctggactt tgtttcataa
     1921 tcgttcctgg ttcgctttcg ctggactctt cttcctctat cttaatcaaa ttctcaggaa
     1981 ccttcttctg cttgagttct gagattactt ctgtagagtt ccgtccaata tagttcccta
     2041 attgaatcgt cgtagctttt ttagctactg tcaaaacaat ttgagttgcc ttgctcaagt
     2101 cataggtcgt accttctggt agactttgct tcaagaccgt tccagcctca ctctcattgg
     2161 actcttcttc ctcaatttta atcaaattat ctggaacttt tttctctttt aattccgcga
     2221 taacatcaga agatttccgg ccgacataat tactaatttg gaaggattgt ttgcctgatg
     2281 agacaaccag attgattttc gttccttctt ttcgaccagt tccagcgcca ggatctgtac
     2341 ggataatccg cccttcttcc accttttcac tagcctctgt cttctcctca ccaatctcaa
     2401 aattggcttt tttgagcgtt gccttggcct ctgcaactgt ctgacctgcc acatccggaa
     2461 tggcaatggt tgcaggagtt ctggataaga tccaaataag agaagctgcc accaatacaa
     2521 ggctggccaa caaaatcagg taacgcatct taaatctatg ttttttcggt gcttgtggtt
     2581 ggtaagtttc ctctgtcaca gcctggcttg ggtttttgat tgatttgtgt tctgtctgcg
     2641 cttgaacctt aggaatagat gtcaaggtac tctgagaaac cttcggcaag gtcttggtat
     2701 ctgccttgct cgtttcatca aagattaact tactttcatt tctacgattg taggacaagc
     2761 tactagacaa gtccacatac atctctgaaa ccgatcggta gcgattggtc aactttttag
     2821 cagttgcctt gataataaca ttttctaaag cctgaggtac agatggattt tctgcaataa
     2881 cggacggcag gggtttctgg aaatgctgga gggcaatggt caccgcgcta tccccgtcat
     2941 aagggatatg gcctgtcaac atctcataga aaataatccc catggcatag atatcactct
     3001 gcacagtcgc cttagaacca cgcgcctgct ctggtgacaa gtaatgaact gagcccaaca
     3061 tcgagttagt ctgggtcaga cttgtctctg caaaggctac agcaatccca aagtctgtga
     3121 ccttggcagt cccatctggt gtcaaaagga tattttgagg tttcaagtcc ctgtgaacaa
     3181 ttcctcgagt atgggccaag cgcatagcca agagaatttg tcccatgata cggacggctt
     3241 cttcattaga aagaggataa tgttccttga tatagcgttt gaggtctagt ccagcaacat
     3301 actccattgc aagatactgt tgaccgtctt cctcaccaat atctgttatc cgaacgatat
     3361 gaggatggtc tagatctgcc atagctctcg cttcacgctg aaaacgagct acagctatcg
     3421 ggtccgtctg gtagttggtc ctcagaacct tcactgccac ttcttcccca tctaagatta
     3481 agtctttggc taggtaaaca tccgccatgc ctcctcgacc aatctgtttg acaatccgat
     3541 agcgtccggc aaaaatcttg ccgatttgga tcattcttca tcctcctcgt tcatagaaac
     3601 aagggcaacc gtaatgttgt ctaaacctcc tgcattgtta gcaaaacgaa caagtgtctc
     3661 cgttttatct gctaaaggaa tatcactggt tacaatatca cgaatctcac tgcctgaaat
     3721 catgttggtc aagccgtcac tattgagcaa gagatagtca cctgactcaa ggataactgt
     3781 cccaaaatca ggctgaattt catctttttg cccaatagac tgggtgataa tatttttttg
     3841 cggatgagct tctgcctctt ctggtgtcaa ttgaccagcc ttgagcaatt cattaaccaa
     3901 ggaatgatcg ctcgtcaact gatggtattc ttctccacga atcaagccga tacgcgaatc
     3961 accaatatga gcatagatag cctgattatc aataatagca aggacttcca aagtagttcc
     4021 catgcctctg taagcttcat cctgaccaag ctggtgaatc ttttgatttt caatttctag
     4081 gtaatgggcg aaccattcac gcacttcatt gactgtatcg atctgggtat caacccaagc
     4141 tacacccagg tctgtgaccg ccatttcact agcgatattc cctgcacgat gacctcccat
     4201 cccatcagct aaaataatca tggtacgtcc agctctattg acatagtggt tgacatagtc
     4261 ttggttattt gttcgtttct gaccaacatc tgttaataat gaaatttcca tgtgtcagtt
     4321 ccttcctaat ccgatatctt gcgaaattga ctgatgaaga atccatcact tccatacaat
     4381 tcaggtgtaa tgaggataca gccgtctttc atgatatcct tacattcatg ttctagtttt
     4441 acctgctcga actcgggatg actctctaaa aaggccttaa cgacttgaaa attctcctct
     4501 gagacgatag tgcaggtgct ataagttatt ataccacctt tgcctagtat ttgacaaaca
     4561 ctacctaata tttctaactg aatttcctgc aaggacgcga aatctgccgt ttctttattg
     4621 tatttgatat ctggttttcg gcgcaaaaga ccgattcctg aacaaggagc atccaccaaa
     4681 atcttatcaa aggaatcctg gtcaaaaaac tcatgcacct ttctggcatc caatttttga
     4741 gtttgaaccc gatcttcaac tcccagacgt tgggcatttt cttgaattaa atccaacttg
     4801 tggtcgtaca agtccagagc agtaacctga cctgtcgtaa gataagaggc tatatgggct
     4861 gttttcccac ctggagccgc acaggcatca agcactcgct catcaccttg taaatcaagc
     4921 gtcggagcaa ccagctgact ggactcgtct tggatggtaa tggctccatc cgcaaacaaa
     4981 ttatgccctg caaaatgccc ctgctcctta accagaccag tggttgctaa aagggaatca
     5041 ttcgcctcca acaaggcttg gatttcctct tttcgactta ggtctgttac acgaatactg
     5101 gctttgtttc gcactaacag gctttcaaag atggcttttg ctctctcctc tccgtattct
     5161 tccttgagtt tggcaactag ccaaactggg agagaatagg caatggagtc acgcttgttt
     5221 tttcgcttga tgctagcaat atctggccag ccttcacgca agatacggcg aaggagggca
     5281 ttgaccaatt tttcactgcc ttttttacga agtttggcca attccactgc ttcattgacc
     5341 acagcatgat ctggaatctt gtccaaatag cggagttggt aggcactcat gagaagaagg
     5401 acatagagcc agctgtctaa ctggtctctg tcttcgataa agtgggatag gtaccattcc
     5461 agagtcagtt tacgggctac cgttccatag accagctcgg tcactaagcc cttgtctgct
     5521 gccaaaagtt gactcccctt tagatgctta tttaaggcga tatttgagta tgcttggttc
     5581 acaaaaacat cctctagcac tgctagagct aaacttctag ccgtttctac tttagtcacc
     5641 aaatcgttct cctacagtca atgtacgtcc aactccgttg aggaaggaag caatgtccat
     5701 cttaggctta ccagctggct gcacttgttt gagggataga gccccttcag ccgttgcgac
     5761 aatcaattct ttcttgccga tagagagaat ctcacctgga tttccctgac cttctactgg
     5821 tagggcttca taaatcttaa agcggtcgcc cttaaggaaa gcatgggcaa caggccaggg
     5881 gttcattcca cgaatttggt taaagagttg acgattggtt ttgttccagt ccagtttttc
     5941 ttcctctggc tttatatttg gagagaaggt aacctgactc gtatcctgcg gttcaggttt
     6001 gatatcacca gcaatatagg caggcagagt gtccaaaagc aaatcacgac caactagcgc
     6061 caatttttca aacaaggtgc caacattgtc ctcatctgtg atcggaatgc tgcgacgaga
     6121 aatcatatct cctgcatcca tttccttaac catttccatg atggtcacac cagcttcctc
     6181 atccccttga atcaaggcat aatggatagg cgcaccacca cggtgtctag gaaggaggga
     6241 ggcatgaacg ttgacagcaa agtccatgct atcaaggagt ttgcttggga gaaactgccc
     6301 aaaagcagca gtcacaattc catctgctcc tagcttcata agttcttcca tctctggact
     6361 tccagataat ttttcaggtt ggtagataga tagtcctgct tccttggcag cctgcttgac
     6421 tggggtttct tggataactt ttttacgacc aacagcacgg tctggctggg tcacaacggc
     6481 tagaatttcg taacggtcat ctgtcaaaag tccttttaag actgttgctg aaaagtcggg
     6541 ggtccccata aagattagtt ttgtcatatc ttctccttct tataaaaatt gctgcggctc
     6601 atggtcaatg ctgagacgga gctcactatt ttcccgttct tgagtcaagg ccaagacctg
     6661 gttgagggtc ggccccagct catcttctaa acggtattta attaaaatct ggtaatgata
     6721 gaggttgtgg gtacgggcaa tcggttttgg cgttggcccc agaatgggac tggtctctga
     6781 caagcctgac cgcaaaatgt tcatgacttc ataggcacgt ttgaaaacct cttcttcttt
     6841 cttgtgagaa agggtaatac caatcgtgaa atagtaaggt ggatagccga gttgtcgtct
     6901 gattcccatt tcataggcat aaaagccttc gtaatcttga tccttggcaa atcgaatagc
     6961 atagtgctgc gaattgtagg actgtatcaa gacttgacct gctttttcag cccgacctgc
     7021 tcgacctgcc acctgagtca agagctggaa ggttctctca gaagaacgga aatcaggcag
     7081 attcaaggcc gtatccgcat ttagaactcc gactagggta acattgggaa aatccaaacc
     7141 ctttgcaatc atctgagtac caagtaaaat atccgcttcc cctcgcccaa actggtcaag
     7201 caaggcttgg tgactgcctt tctttcgagt cgtatccaca tccatcctca aaatgcgagc
     7261 ttggggaaag agttctgcta gctcatcata agccttctga gttcccgtcc catagtaacg
     7321 aatactgcgg ctcttacagt taggacagac ctgaggaata tccttcgaga aaccacaata
     7381 atggcagttc atagtcttgg tatccatatg caaggtcaga gaaatatcgc agttgggaca
     7441 agtatccacc gtcccacact cccgacacat gacaaagcta gaataaccac ggcgattgag
     7501 catgagaacc acctgctctt ttttaaccag acggtcttgg atagcctcta gcaaaggagg
     7561 cgtaaagttt gacgtctcat tttgtccgat atagtctcga aagtcaatca cttgaacctc
     7621 agggattgta gccaaaggat tagcacgttg ggttagacgt aagtgttgat agacgccttt
     7681 gccagcacgt gcccggctct ctaagctcgg cgttgcagat ccaagtacca gagttgcttg
     7741 attatactga gcccgtaaaa tagctacatc tctggcatgg taacggggat tgctgtcctg
     7801 cttataagcc acttcatgct cttcatcaat aatcatgaca cccagatttt tcagaggagc
     7861 aaagatagca gatctggcac caacaacaac ttgggcatcg ccacgctcca ccttgcgcca
     7921 ttcatcatac ttttcaccat tggataatcc tgagtgaaga atggctacct tgtccccaaa
     7981 acgtgctata aaacgctcgg tcatctgagg agtcaaggaa atctcaggta ccagcaaaat
     8041 agctgtcttg cccttatcca gggcaccttg gataatctgc aagtaaacct cggtcttccc
     8101 acttcctgta atcccttgaa gtagaaaggg aggttgagaa ctgccaatag aactcacaac
     8161 cgcatcacgc gcctgtcttt gttctggatt taactccaaa ggtctacttg cttcaattcc
     8221 ttcaaaataa gcagccgagc gttgaacttc cttttggact atggtaacag caccttgatc
     8281 cacaaagaag ttgacttgct ctcgcgagta ggactctaac aagctagcca aggaagcgct
     8341 ctctggatga gacagcagat aatctctcag ttccaacttt ttcttggcac gtgtagaaat
     8401 ctcaacacct tctaattgag catggtcaac ctcataccaa gactgggtct tgaccttctt
     8461 ttgatcgact gcctgatatt ccagaccaag caggcctttt ctagtcaaac gcatcatttc
     8521 agcttgcttg gcaaggtcta gtgaagaaaa ggctagcgaa tcttctgaac caaacaggcg
     8581 cactcgttct tcctgactca agccttccag aggatagaga atcttgtcat agctagaatt
     8641 cagaaatcct ggaagcatgg ccttgaggat agaaattttg taggagaaga cagatttgcg
     8701 taactcctca gccagccaga gttgttctgg cgtgagaaca ggagaaaaat ccagcacctc
     8761 tgcaatatct tttaaatctt gctccatctc ttctccatct gattgggact ccaaaccaag
     8821 aacaatccct tgaatcaggc gattcccctt accaaaaggc acatgaaccc gcatcccaac
     8881 ttccagcatt ccctcaaatt cctccggaat cctgtaacta tagggctggt ccgtctgcat
     8941 caagggcaca tctacgataa tcttagctag ggccatcgtc tcacctcctc cttgtcagta
     9001 cattcttgca atagaaaaaa taagattgag tccccccaac cttaaatttt ttcaccatct
     9061 tctttttctt tagcaatttg ctctttgatt ttcttttctt cttcttcttt gcggcgtttt
     9121 tcttcttcga tacggcgacg cactgcttca cgttttcctt ctggatctgg gtgaattgta
     9181 acgtttcctg attcgatttc ttctaaagcg cgaagagttg atttttcaga cttaaaacct
     9241 tgagttgctg gggcacctgc ttccaattcg tgggcacgtt ttgcttccaa gattacgagt
     9301 gaatattttg aaggaacctt gtcgagcaag gtatcaatag agggttttaa catcatttgc
     9361 ttgtacctat tttctaaatt ttatcgggta gttggagatt ttggtaacat ctcctgatag
     9421 tgaccaatga cacgatccac acagaagtgt tctgcttcaa tcacacattt gacacgttca
     9481 gcagctaggg gtacctgatc gttgacaatc gcataatcat actcacgcat gagggcaatt
     9541 tcttccttgg ccttttcgat tcgttgggca atcacttctg cactatctgt tccacgacct
     9601 accaagcgat cttgcaattc atccaaatct ggtggtgtca ggaagataaa gacagcatct
     9661 ggaacctttt tcttgacctg aagagcaccc tgaacttcaa tttcaaggaa aacatcgatt
     9721 cccttgtcca aggtttcatt gacataggtc agaggagttc catagtagtt gccgacatat
     9781 tctgcgtatt ccaacatctg tccttgacga atcagctctt caaattcttc acgagtacgg
     9841 aagaaatagt caacaccgtc cacttctcca ggacgttgtg cgcgtgtcgt catcgataca
     9901 gaatattgaa attggttttc agaactctca aaaatctctc ttctaaccgt tccttttcca
     9961 acccctgaag gaccagaaaa aacgattagt aagcctcggt ctgccattgt gtctcctttt
    10021 agtcaatctg tgaaataaca tttctctaga ataatggcaa aaagccagat tatcctttac
    10081 agtctttcta tctagtgtaa caaaaaagca gtaatttttc aactgctctt tcttatttat
    10141 ttagcataat ctactgcacg aagctcgcga atcacggtta ccttgatatt tcctggataa
    10201 tcgagattgt tttcaatttt cttacgaact ttgtgagcca agattgtgac tttgtcgtcc
    10261 ttgatttttc ctggattgac catgatacga atttcacgtc ctgcttgaag ggcaaagcta
    10321 gtttgcactc cttcaaagcc gttagcaatt tcttccaaat catggagacg cttgatgtag
    10381 ctttcaagag actcactacg agcacctgga cgggctgcgc tcaaggcatc tgctgcagcg
    10441 acgataactg ctatcacgct ctcagcttca acatctccgt ggtgactagc aatcgtattc
    10501 accacaactg ggggttcctt gtacttacgg gccaattcca taccgatttc aacgtggcta
    10561 ccttcaacct catggtcaat ggctttcccg atatcgtgaa ggaatccagc acgacgggca
    10621 agagccgcat tttcaccaag ttcgctcgcc atgataccag ccaacttagc aacctcaatc
    10681 gaatggcgca aaacattttg tccatatgaa gtacggaact gcaaacgtcc cataatcttc
    10741 atcaagtctg gatgaaggtt tggcgcacca atttcatagg cagcagcctc accgtattca
    10801 cgaatcttat tgtcaatctc ttgacggttt ttctcaacca actcttcgat acgagctgga
    10861 tgtatacgac catctttgag caacatttcc atagtcatac gggcaatctc acgacgaatc
    10921 ggatcaaatc ctgacaaggt caccacttct ggtgtatcgt cgataatcac atcgacccct
    10981 gtcaaacttt caaaggtacg aatgttacga ccttcacgac caataatgcg tcccttcata
    11041 gtatcgtctg gcagatgaac tgttgagttt gttgactccg ctacatattc accagcgata
    11101 cgttgcatag cttgaaccaa gatgtccttg gccattttgt cagaacgttc cttgacctct
    11161 tgctcagctt cgcgaatgcg actggcaatc tccctggtca agttttcctc tgtctgagcc
    11221 aagataatat ctcgtgcttc tgcctgagac agagcaccaa tacgctctag ttctgcttct
    11281 ttttgtcttt cgacttcctc taattgctct tcacgcgcat caaggttttt cgctctatca
    11341 gaaatacttt gttctttttg ttcaagtgtt tgttctttac tcgtcaaatt gtcgtcctta
    11401 cggtcaaggc tagtagctct ctctgtcaaa cgactttcga tttgtttgag ttcttgacgt
    11461 tctgatttga attcagcgtc cacttcttca cggtattttc tggcttcttc tttggcctcc
    11521 aatagtgctt cttttttaag agacttgctt tcacgtttgg cttcattaac aagtaaatcc
    11581 gcttcacgct cagcttgtcc acgtaaatta gttgcttctt gttcagcatt taaaagcatc
    11641 aactctgcag cttcctgaga tgatttcatc ttagctgaga tgctgacata tccaatgact
    11701 aaaccaatga tgacggcaaa aacagcaatc gcaagcgaca tgatttccat gtttttacct
    11761 cattttattg ttattccgaa tgacatacat tcttttacat tctaccataa aaaaagaatt
    11821 taaacaaacc ttaaatggat atcgctaaca tccgcattat cactatcata gttatcattt
    11881 acagatataa agcgaatacc tttttcagga aatatctttt gtaagtactt tcctgactcg
    11941 atataatctc tgccgaaacg agataaatct tttaccataa tgatagaaaa ctttccttta
    12001 ttcaggtcat caatcatatt cttaaaattt ggtcgctcaa aattagaacc actatatcca
    12061 tcatctacat attcatagga taattcaata gatttctctt ctacctccaa aaagcaaaaa
    12121 ggataaattt cccctacact ttaatagtga aaatttatcc ctcttggtaa gctcttttat
    12181 taattttttt gataaagaaa acgcacattt tatttaactg gtgctgaaat ctttataata
    12241 aaattttttt ggttctttct agaataatga tattaagatt tttcatatat cttatatatt
    12301 cttcttctgt gctagcaatc aaaatatctt ctttatctaa aaagacaacc tgattgttct
    12361 ctaaatctga tttaattaaa tctataattt tattttgctc atatataaat ccttctaact
    12421 tgaaaaactc gacaaagttc tcaaatccat ttttacaatt atcatcacca gaaaatagtt
    12481 caaaccattc atggtaattc tctatagcat aacctatttt tgtattttta ttttccacaa
    12541 accattcttt tatagcatat agtgttaaat caaaacgatc ataatatcct ttttctccac
    12601 ctctagcctg attaacggtt tctaattttc cacctctagg gaaaagaatg tgtccaccta
    12661 ttgttcttga aatactcaaa tgatctaaaa ttattctgtc gtttaatcct gcctcttgtg
    12721 cccaataaat agaggctcct atataatcac ttgataataa atacttatcc ttatcaataa
    12781 ataaagtata ataatctcta tttccatagt tttggcattt attacataca gatgttattt
    12841 taagtttttc ttttgaatca tcatcaaaaa acatcttaca taaatcatcg tacaacgcct
    12901 tactacatac atcaggatcc tctctattat ctaatgaaaa acctttggga aaataaggat
    12961 ataaatcaaa tagataattt ttaaacttca tatttattat tctccattaa atctttttag
    13021 gaaatttaaa gtcaaatttt cctccaagca cacccgcact tactcccgca actactacaa
    13081 gtgttgtact aattgttcct attttcttca cgccactgtt tagctctttt gttaaataaa
    13141 acaacattga tcatatctgc ttcacttaca tacttgtaag acaactgttc gttggtaaga
    13201 tcttttaaga gatattcttt gattgcatct gtatgaattt tgtagttaat cttagaaatt
    13261 tctctattca aattccagcc taatgatagc ttagaatttt aatctgactt aagtcttttg
    13321 taatcctgaa ttacatacat cttaaattca gcagaaatac ataaagcaaa ttcaaaggca
    13381 atatctgtat gtgcaaatgc accaccatat cttccacctt ttgagataat tccgattgct
    13441 ccagttttct ctacccattt cttaggagac aatgtaaatg aattatatcc agcttccgat
    13501 ttaattcggt cgaattcgac cgaattgaaa ttcatgttat ttaagctttt ccataaacca
    13561 agaaactcaa ttgtatcttt tcttctcatc caatttttaa ttacatcact cgatacttat
    13621 ccgaacgata agctccacgt aaggtgttcg caatcgacca aactgcattg actacatttg
    13681 aaatctgaac atcgttcgac tggaatatcg gtagtttttc tttaaaatta gacatttgct
    13741 tcccctgttt ttatgattta caattctctt cattatacca caaaataggc gctttgtcaa
    13801 ctgtagtggg ttgaagtcag ctaagctcga gaaaggacaa atttcgtcct ttcttttttg
    13861 atgttcaaag cgataaaaat cc
//
LOCUS       DBERYS010000045        13526 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.045, whole genome shotgun sequence.
ACCESSION   DBERYS010000045 DBERYS010000000
VERSION     DBERYS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.045"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(778..1647)
                     /gene="gla"
                     /locus_tag="ACL8LA_001658"
     CDS             complement(778..1647)
                     /gene="gla"
                     /locus_tag="ACL8LA_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGS1813387.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1871..2392)
                     /locus_tag="ACL8LA_001659"
     CDS             complement(1871..2392)
                     /locus_tag="ACL8LA_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1813388.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2350..3456)
                     /locus_tag="ACL8LA_001660"
     CDS             complement(2350..3456)
                     /locus_tag="ACL8LA_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGS1813389.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVVVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3567..3821)
                     /locus_tag="ACL8LA_001661"
     CDS             complement(3567..3821)
                     /locus_tag="ACL8LA_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813390.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(4161..5006)
                     /locus_tag="ACL8LA_001662"
                     /pseudo
     CDS             complement(4161..5006)
                     /locus_tag="ACL8LA_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            5240..6958
                     /locus_tag="ACL8LA_001663"
     CDS             5240..6958
                     /locus_tag="ACL8LA_001663"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGS1813391.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7069..7416
                     /locus_tag="ACL8LA_001664"
     CDS             7069..7416
                     /locus_tag="ACL8LA_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS1813392.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7695..8531)
                     /locus_tag="ACL8LA_001665"
     CDS             complement(7695..8531)
                     /locus_tag="ACL8LA_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1813393.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8544..9173)
                     /locus_tag="ACL8LA_001666"
     CDS             complement(8544..9173)
                     /locus_tag="ACL8LA_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1813394.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9183..9824)
                     /locus_tag="ACL8LA_001667"
     CDS             complement(9183..9824)
                     /locus_tag="ACL8LA_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1813395.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9970..11199)
                     /locus_tag="ACL8LA_001668"
     CDS             complement(9970..11199)
                     /locus_tag="ACL8LA_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS1813396.1"
                     /translation="MNNSQQMLQALEEQDLTKAEYYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11189..12355)
                     /locus_tag="ACL8LA_001669"
     CDS             complement(11189..12355)
                     /locus_tag="ACL8LA_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGS1813397.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVIFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12499..13512
                     /locus_tag="ACL8LA_001670"
     CDS             12499..13512
                     /locus_tag="ACL8LA_001670"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGS1813398.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNEFHVPEAVCIR
                     FAP"
BASE COUNT         4122 a         2907 c         2381 g         4116 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatac ccccctaatc
      361 gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag gtaaatcaat
      421 tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag
      481 acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac tgcacaattt
      541 gattggagaa ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa
      601 ctgcaaccta ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc
      661 tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca
      721 gtctgatcct ctcttattta tagatggtgc tcactcaaat gttaaaggag ctatttctta
      781 gagataaagg aatttgaata cagctactgc cgcaattgct gctacgatag gtgctactac
      841 tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgattttgg
      901 aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt
      961 aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac
     1021 agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc
     1081 aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt cagcaccaaa
     1141 gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt
     1201 aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga
     1261 aatagttgag aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg
     1321 gtatgttgcc acaactaagg cttggccaaa gatagctccc aagacttgcg cgataatata
     1381 aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg
     1441 gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc
     1501 ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac
     1561 gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc
     1621 atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt
     1681 ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat ttttaaaaaa
     1741 ataggattca tagtttccca gttcaacgga cttgtaagcg tttttattct tcacgaaaga
     1801 cctcgttctc aaaaccagga aaaaagccca catctgtgag cttttcctgg ttcctagatt
     1861 ctaaaatcta ttatttttca tcttcttttt tcttgaagaa tcctaatcct agactagcta
     1921 acaaggtcat gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca
     1981 attcctctct cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt
     2041 cagtttttgg agtacctggg tctggagttg gtttatctgg tgttgataaa cggtcatacc
     2101 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     2161 tatctggatc tttgtacttg tcaatcttac cagatacaac ctcgtcccag tttcctgttg
     2221 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     2281 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     2341 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     2401 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     2461 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     2521 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     2581 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     2641 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     2701 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     2761 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     2821 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     2881 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     2941 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     3001 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     3061 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     3121 acggtgactt tgagtgttta ctactactct ttcattattc tctgaaaata cttgtacggt
     3181 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     3241 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     3301 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     3361 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     3421 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     3481 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     3541 ttgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     3601 gtcttcagta gtttatatgc tctgtcatca tctttagctt tagaagcagc tgagtttagc
     3661 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     3721 gctagcctat tggttgtcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     3781 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     3841 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     3901 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     3961 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     4021 tcaaaccttt cttatacaag tcttcattct aaacgatttc ggctacacgg tgaatggttt
     4081 gattgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgatagg
     4141 ttcctttaga accgttatag tcatctagtt ttaaaaaagc attccaaaca ttcaaaaata
     4201 gttggaactt cccttaataa atccgtcact ttctttttca agatagccca agcttgctca
     4261 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     4321 cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac aataacatga
     4381 ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     4441 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     4501 gaagttcgct caaaacgacg tccgctaatc tttttataga ctttctcccc tctaggagcc
     4561 cgtgcataag gacgatagag gtagcggtcg attcctgttt catcaatata tacgattggt
     4621 aaatctttta ggttatccaa aatatcaaga aactcagcga ctttctctgg ctcttgttcc
     4681 ttaaagctcg tcatcttttt ttaaagtgac cttaatatac tttaaagctg cccatacgga
     4741 gggcagagca caatcaaaat gtgccgcaat ttcccgtaaa aaagcatctg gatgagcctc
     4801 tacaaaggct ttcaattcct ctaaaggaat ctttcggttt ttgacgactc gctttttcct
     4861 ctctatgtgt ccttgttcac atctttcttt tcccacgtga agagagttct gacgccaaca
     4921 ccaaaaaact tggctgcctc gacatggctg tgcccctctt tgatgtaatc taatgctcgc
     4981 tgtttaaaat cagtactata tgccatagct ttattataat atgcttgttt aaaactaagc
     5041 aactatatca atgttttgtt attagaagac ttactttttt tactgaaatc gaatttttcc
     5101 taacctcaac atcatataac cccaaaattc aatctgacct ggtttgaaga gatgttcgaa
     5161 gcagataagg ttttactaaa actaaaaaaa atgatataat caaactgatt aaatgtttta
     5221 aaaataaagg agaatttgaa tgtcttacca agaaaattac cagaaatggg ttgattttgt
     5281 ggagcttcct gactaccttc gtcaagattt ggaaaatatg gacgaaaaaa ctaaggaaga
     5341 tgccttctat acaaatcttg aatttggtac tgcaggtatg cgtggcttgg ttggtgctgg
     5401 tacaaaccgt atcaacatct acgttgttcg acaagctact gaaggattgg ctcgtttgat
     5461 tgagtcaaaa ggtggaaacg agaaagaacg cggtgtagca attgcctacg atagccgtca
     5521 cttctcacct gagtttgcct ttgaatctgc ggcagttctt gctaaacacg gcatcaaatc
     5581 ttacgtattt gaaagccttc gtccgactcc agaactatca tttgcagttc gtcatctcaa
     5641 ctgtttcgca ggtatcatgg tcacagccag ccacaaccct gctccattta acggttacaa
     5701 ggtttacggt gaagacggtg gacaaatgcc tccacacgat gcggacgctt tgactactta
     5761 tatccgtgca atcgaaaacc catttgcagt tgaagttgct gatgtggaaa ctgaaaaagc
     5821 ttctggcttg attgaagtta tcggcgaagc tgttgacgta gaatacctta aagaggttaa
     5881 ggacgtaaac atcaacccag ccttgattga agaatttggt aaagacatga agattgtcta
     5941 cacaccactt catggtactg gtgaaatgtt ggctcgtcgt gctcttgccc aagcaggatt
     6001 tgactctgtt caagttgttg aagcgcaagc aactgctgac ccagacttct caactgtaac
     6061 atctccaaac ccagaaagcc aagcagcctt tgcccttgct gaagaacttg gtcgtcaagt
     6121 tggtgcagat gttcttgtcg caactgaccc agacgctgac cgtgttggtg ttgaagttct
     6181 tcaaaaagat ggtagctacc tcaacctttc aggtaaccaa atcggtgcta tcatggctaa
     6241 atacatcttg gaagctcaca aaaacgctgg aactcttcct gaaaatgccg ctctctgcaa
     6301 atctatcgtt tcaactgact tggtaacgaa gattgctgaa agctacggcg caactatgtt
     6361 caacgtcttg acaggtttca aatttatcgc tgaaaaaatc caagaattcg aagaaaaaca
     6421 caaccacact tacatgatgg gatttgaaga aagcttcggt tacttgatta aaccatttgt
     6481 acgtgataaa gatgccatcc aagccgttct tgtcgttgct gaacttgctg cctactaccg
     6541 ttctcgtggt ttgacacttg ctgacggtat cgaagaaatc tataaagaat atggctacta
     6601 cgcagaaaaa acaatctctg ttactctttc aggtgtcgat ggtgctgaac aaatcaaagc
     6661 gattatggct aaattccgca acaatgctcc aacagaatgg aacgcaacag ctatcactgt
     6721 cgtagaagac ttcaaggcac aaactgctac tgttgctgac ggtactgtta caaacttgac
     6781 aactcctcca agtgatgtgt tgaaatacac acttgctgac ggttcatgga ttgccgttcg
     6841 cccttcaggt acagaaccaa aaatcaagtt ctacattgca gttgtagggg aaaccaacga
     6901 agaatcacaa gctaagatta ctaacatcga agcagaaatc aatgcatttg taaaataaag
     6961 acctataaaa gatgagacca accaacctca tcttttttcg tatcaaatct aagcaatttt
     7021 agaaacttta tggcatacta gacttatcaa tgaaagcgag gtaatctcat ggaacaattt
     7081 ttagataaca tcaaagacct tgaagtcact acagttgtgc gtgcgcaaga agctcttgat
     7141 aaaaaagaaa ctgcaacctt ctttatcggt cgcaaaactt gcccttactg ccgtaaattt
     7201 gcaggtacat tgtcaggtgt cgtagctgaa accaaagctc acatttactt catcaatagt
     7261 gaagaaccaa gccaactcaa tgatttgcaa gcattccgct cacgttatgg aatcccaact
     7321 gtaccaggtt ttattcatat tacagatgga caaatcaacg tccgttgcga ctcttcaatg
     7381 tcagcacaag aaatcaaaga tttcgcagga ttgtaaaaaa tcagctaaca agtcttaaaa
     7441 tcacaaaaac gcataatgta aggtgcaaaa accttgatat tatgcgtttt attgttccta
     7501 aaacagttac atcagcattt cattacagaa cattttaatt ttggaagtca aatcaatttt
     7561 taacaatgtt ttagaaatca cagtgggcta ttccatattc aattatccag attcaatcgc
     7621 tattatcgtt ttcctagatt acaaaaaaaa ctacgttttg ctaatcaaaa cgtagtctca
     7681 gccactattt tatcttactg tccttctttt acttctttgg ttgctacatc ttctccaaac
     7741 catttttggc tgatttcttg gaacttgccg tccttgtaaa gactagaaaa agcttcattt
     7801 atcttcttaa ccaaggttgt atcttcctta cgggctccaa ccgcaaaagc ttctgtttct
     7861 agtccaactg taaagacatt ataatcgttt aaaacacctt ctgcttctaa ataatagttt
     7921 gcatagacac ggtcaatcaa tagaccatca attcgatcgt ttttcaaatc aatcaaggct
     7981 tcattaaagg tttggtattg attcgcttcc ttattagcga caatattctt caaaatttct
     8041 ggatttgctt caaagtccgc ataaccagat gaaccagctt gagctcctaa tgtctttcca
     8101 gtcatatcct ttgcagtcgt gataccagat gattttttcg taaccaatac ctgctcattc
     8161 ttcatatatg agttactgaa agccaccttt tcacggcgtt cgtctgtagc ggaatagcca
     8221 ttccaaatca gatcaatcgt tccttttgtc aattcagctt ctttcaaatc ccaatcaatc
     8281 ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag ctgtagctaa atcaatatca
     8341 aatcctgcat aagaaccatc tttctgagca aatcccattg gaacaaaagt actatcaaat
     8401 ccaatagtaa tagacttgtt agactggtac tttgaccaat tatctccact agtttcgcta
     8461 gaatttttcc cacaagctac taagaacaaa gcagtcatca gactgactaa tacaagcatc
     8521 cattttttca tccatttttc ctcctattta ggttctactt tcaataatac atctgcgata
     8581 ttttcagcaa actgcaaatc atgggtaacc acaatctggg tcatcccaag ttccctattt
     8641 tgcaagatta gcttctccac ttccaaacgt aattctggat ccagggcaga agttggttca
     8701 tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg ccaaagccac ccgctgcttt
     8761 tgcccaccag atagtgagaa aggataggcc tctgcgtgtc ctcctagtcc taactgttcc
     8821 aagagtccac tcgccttctt ctcagcctct tcctgcttca ttcccatggt cttcacaggc
     8881 gataaagtca aattttccag aactgataga tgaggaaata gttgaaaatc ttggaagaca
     8941 aatcctagta ggttgcgctt ctgcaattca tccagctcta aaggttgtcc attataaaag
     9001 atttgccctg aatcaatggt ttcaagaccc gcaagcatac gtaaaagagt cgtcttacct
     9061 ccaccagaag gtccaacgat agccaggatt tgcttttcag gaatacttag actgaaatta
     9121 gacaggattt gtttgtctcc aaagacttta ttgatatttc gtaattctaa catggcagcc
     9181 tcctatctat aataactata cttcttctca acttttttgg aaataattgt cacaatccca
     9241 atcaaaatca aataaatggc tcctgccaag aacataggaa ctagactagc atcgcggtta
     9301 gcagctgttc gactagccaa gataaggtct gaaattccga gagcatagac caaagaagta
     9361 tccttgacca aactcataac ttcattaaag acactaggaa gaacgatctt ggtcacttgg
     9421 ggcaagataa tatagcgcac tctgtcaaaa gggctaaact tcaagacctt ggcggcctca
     9481 tactgtcctc ttggaatagt gtcaattccc ccacggaaaa tttctgcaaa gtaagctgca
     9541 tagttgagaa caaaggcaat aatagctgca ggaaggcggt ctaaacgaat cccaatactt
     9601 gggagcacat aatagataaa aattagttgc aagagtaagg gggttcctcg cataacccag
     9661 atgtaaatgt taatcagata atggaggggc ttccaatgga cttgcaaggc aaaagcaatc
     9721 aaaacgccca agggaatcga aaatagcaag accagtgcaa agacctgtac agtcgtgctc
     9781 gcaccattca gtaaactcgg taatatctca aataaataag acatactgca cctcctaaaa
     9841 gtaattgttc ctattatagc atgaataaac aagattacca agtatttttg taaaaagatt
     9901 ctaccatata caaataaaaa ccgtagctat caaaagagat aaactacagt tctatatgtt
     9961 taaacattct tacaatctct caaacagttc ttgcatttgc acatcatctg gaaccagttt
    10021 taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt cccaattcac gcaagagata
    10081 gatatagtgt tccagaaatt ctggattgtc cttcaaatct cctgtcaact cttgataatg
    10141 ctcataagca gtatccaaat cgtccatttc ttgataagaa cgagcaatca tccacttggt
    10201 caaaagattt tctggctcct cactctgcaa gtctagaata tcctcataac gctcctgctc
    10261 cagataaata gtggctaaac gaagcaagat ttcttctgta tcctcagcgt cttcttttgc
    10321 agcaagaaga taattttctg caccactagc atcatgcaat tcataagaaa attgtgaagc
    10381 agctagcaag aggcgagttt caaagggatt tttctctaat ccttgcttag cgatacgcag
    10441 ggcttcttga acttgatgtt ccttatgtaa agcctgactg tacccatact catagccttc
    10501 aaagtcagga gaaatggtat caagctgctt aaagtagagg gtggcttttt gatattcttc
    10561 ttgatcaaaa taaagactgg ccaactcaaa agctgttaag tcatcgtatt ctaactccag
    10621 ggctttttct aaaaactcag tagccgtttc aaatttccct aactgagcat aggcaaagcc
    10681 aattcgttga taggtggaaa tgcccgtttg ctcataaatc gagcgattat ctaactgggc
    10741 ataggcttga atagccgctt ggtaattttc caactcacta tccaactctg ccaaacccaa
    10801 catcaagaga gaatcctctg agtaggtcaa ggcctccaat aatttctcac gtgccacatc
    10861 tgtcaaacct tccagctggt aaaggtctgc cttcaaaacc aaagacgaga cataccagtc
    10921 actgtcagct tggatttcct caagataggt aaaggcttct tctatttgac catcctcgct
    10981 agcaattgca gctagattaa gatgaacctc tggaaaatcc tctacaattt tcaggtaaat
    11041 ttccttggcc tgaggataga aaccaatccc ttcaagataa gttgccaact catacagaag
    11101 atcacttgaa tcattttcta aagctttggc gaaataatac tcagccttag ttaaatcttg
    11161 ctcctccaaa gcctgtaaca tctgttgact attgttcact cttgacttcc tcaccctcta
    11221 attcatatag accactgact accttatacc attcgaaaat ggctgaaatg acaaccttag
    11281 cagaggcata aaccggaata ccaagtaaaa ctccccagat accaaacata gatcctgaag
    11341 ttaacaaaac aaagagaaca ttaatagggt ggatgtttaa ttgacttccc aaaatcaatg
    11401 gagagacaaa acggccttca atagtttgtt ctacgataaa gacaatcact actttcaaaa
    11461 gcatgactgg accagcaatc aaacccaata ctagagcagg aagcatggct agaaagctac
    11521 caagataagg gaccagattt aaaataccag cagtaacccc cagcgtaacc gcatagcgta
    11581 gaccaataat cttgaagaag atgataaata ttactgctac aataatagcc actgtcactt
    11641 gccctcgaac atagttggac aactgttgat tcacatctga taaaacttgt ccaacaggtt
    11701 ccttcaattt tcttggaatg aattgggtca aatagttacg caagcctttc ccatcacgca
    11761 agagataaaa gagcatgaaa ggaacgataa tcaaggcaac aatcacttga gaagctccac
    11821 taataaaggc actcacccag ttgactgcct gagatgaaac cttacttgcc aaaactgtag
    11881 cctggctaga aaaattggtc aaaacttgct ctaattgagg tctgaaatca tctggcaggt
    11941 gctgggctac caatccatta acaatcctat ctatatcttc taagtaaaca ggaacgtttc
    12001 ttgcaaaggt caaaacctga cgttgcagat ttggaatggc gactgccaag ccccaaatga
    12061 taaagagagc gatgataaca aagacaatag tgatagctat aacacgatta accttatgct
    12121 tctccatcca atcaacaata ggattcaaca aataatataa caaaccagac aaaatgactg
    12181 gcaacatcac aactgctaaa aaatctaaaa caggtgaaaa tagaaaacta atcttactta
    12241 aaataaaaag attcaatccc aataataagg ttactaaaaa taccgtaatt gccttgttat
    12301 ctaaaaacca cttgaaaaac caagataggc taaaatgttt ctctttttgc tccataaata
    12361 cttcctttct attgttctct cattatacca tttttgaaac aaaataactc ttttttaaag
    12421 tgcttacatc taattgaatg tctttaattt gcttgttttt gtgatataat agtcttatct
    12481 ttacacagag gtatagtaat gaaagaaact gtttattttg gaacttatac acgtcgtact
    12541 tctcaaggga tttacaaggc agactttgat acagaaactg gtcagctttc aaatctagaa
    12601 ctttttgcag ctgagccaag tccaacctac cttgcctttg accagcacca acatttatac
    12661 actgttggta gccaagacga taagggggga attgcagcct atcaaactga cgggactgtg
    12721 ttaaatcatg ttgttgaaga aggagctccc cactgttatg ttgctgtcga tgaaaagcgt
    12781 gatttggttt acgcagccaa ctatcacaag ggacaagtcc ttgtttataa acgccaggaa
    12841 gatggtagtc ttctacttag tgatatggat caacacagtg gccaaggtcc acatgaaaat
    12901 caagcttccc cccatgttca ctatacagat ttaacacctg accactatct agtgacctgc
    12961 gacttgggaa ctgaccaagt catcacctat gacctcgatc aagaaggaaa attatctaag
    13021 ctctatacct atcacagcaa gccaggagca ggctcacgcc atatcatttt ccataaccac
    13081 tataaaatcg cttatctcat ttgtgaactc aatagtacta tcgaagtttt aatctacgat
    13141 ggcgttggcg aatttgtacg tatgcaggtt atttcaactt taccagaagc ttacgaaggc
    13201 tttaatggaa ccgctgctat tcatctctct aaagacggta aatacctcta cgcttctaac
    13261 cgtggccatg attctatcgc agtatatacc atccttgcgg acggtagctt agagttatta
    13321 gaaatcgttc caacgcatgg tcagactcca cgtgattttg atttgacacc cgaccaaaaa
    13381 tttctcattg ttgtccatca agactctgac aatgcaactg tctttaaacg taattgtgac
    13441 aatggtcgtc tagcagaact ctccaacgaa ttccatgttc ccgaagcagt ctgcatccgt
    13501 tttgctcctt aaaacaccat aaaaaa
//
LOCUS       DBERYS010000046        13380 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.046, whole genome shotgun sequence.
ACCESSION   DBERYS010000046 DBERYS010000000
VERSION     DBERYS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.046"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            72..1331
                     /locus_tag="ACL8LA_001671"
     CDS             72..1331
                     /locus_tag="ACL8LA_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGS1813399.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1407..1542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1581..2081
                     /gene="rplJ"
                     /locus_tag="ACL8LA_001672"
     CDS             1581..2081
                     /gene="rplJ"
                     /locus_tag="ACL8LA_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGS1813400.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2157..2525
                     /gene="rplL"
                     /locus_tag="ACL8LA_001673"
     CDS             2157..2525
                     /gene="rplL"
                     /locus_tag="ACL8LA_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGS1813401.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2712..2882
                     /locus_tag="ACL8LA_001674"
     CDS             2712..2882
                     /locus_tag="ACL8LA_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813402.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2882..3130
                     /locus_tag="ACL8LA_001675"
                     /pseudo
     CDS             2882..3130
                     /locus_tag="ACL8LA_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3144..3484
                     /locus_tag="ACL8LA_001676"
                     /pseudo
     CDS             3144..3484
                     /locus_tag="ACL8LA_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3477..3857
                     /locus_tag="ACL8LA_001677"
     CDS             3477..3857
                     /locus_tag="ACL8LA_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS1813403.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3870..4103
                     /locus_tag="ACL8LA_001678"
                     /pseudo
     CDS             3870..4103
                     /locus_tag="ACL8LA_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            4106..4693
                     /locus_tag="ACL8LA_001679"
     CDS             4106..4693
                     /locus_tag="ACL8LA_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1813404.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            4895..5032
                     /locus_tag="ACL8LA_001680"
     CDS             4895..5032
                     /locus_tag="ACL8LA_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS1813405.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            5211..7820
                     /locus_tag="ACL8LA_001681"
     CDS             5211..7820
                     /locus_tag="ACL8LA_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS1813406.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKSMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            7853..9793
                     /locus_tag="ACL8LA_001682"
     CDS             7853..9793
                     /locus_tag="ACL8LA_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGS1813407.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGISSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRAPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAADHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            9790..11397
                     /locus_tag="ACL8LA_001683"
     CDS             9790..11397
                     /locus_tag="ACL8LA_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813408.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            11412..12152
                     /locus_tag="ACL8LA_001684"
     CDS             11412..12152
                     /locus_tag="ACL8LA_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813409.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            12155..>13380
                     /locus_tag="ACL8LA_001685"
     CDS             12155..>13380
                     /locus_tag="ACL8LA_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813410.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDILIDDMNLLGLIL"
BASE COUNT         4484 a         1879 c         2640 g         4377 t
ORIGIN      
        1 acacctacgg caaagtgaat ctgaagtggt ttgaagagag tacaacttgt cttttaaaaa
       61 aggagtctat aatgaaagtc tttcagcatg taaatatcgt gacttgtgat caaaatttcc
      121 atgtttatct tgatggaatc ttagcagtca aggattctca aatcgtctat gtcggtcaag
      181 ataagccagc gtttttagaa caagctgagc agattataga ctatcaggga gcttggatta
      241 tgcctggttt ggtcaattgt cacacccatt ctgcaatgac aggtctgaga gggatccgag
      301 atgacagcaa tctccatgaa tggctcaatg actatatctg gccagcagaa tctgagttta
      361 ctcccgacat gactaccaat gcggtcaaag aagccctgac agagatgctc cagtcaggaa
      421 caacaacctt taacgatatg tataatccca atggtgtgga tatccagcaa atttatcagg
      481 tggtgaaaac ttccaagatg cgttgttatt tttctccgac tctcttttct tcagagacag
      541 aaacaactgc tgagactata agcagaactc gatccatcat agacgaaatc ttaaaatata
      601 aaaatccaaa tttcaaggtt atggtagcac ctcattctcc gtatagctgc agtagagact
      661 tgctggaagc gagtttggaa atggcaaaag agctaaatat tccgctccat gtccatgtgg
      721 cggagaccaa ggaggagtca ggaattatcc tcaaacgata cggcaaacgc ccccttgctt
      781 ttcttgaaga actgggttat ttagatcatc cgtccgtatt tgctcacggg gtcgaattaa
      841 acgagagaga aattgaacgc ttggcatctt ctcaagtggc tatcgcccac aatcctatca
      901 gtaacctcaa actggcatca ggaattgctc caattatcca gctccaaaaa gcgggagtag
      961 cagtcggaat tgcgactgac tcggttgctt ccaataacaa tctagatatg tttgaggaag
     1021 gaaggactgc agctcttctt cagaagatga aaagtgggga tgccagccag tttccaatcg
     1081 aaacagctct caaggtactg acaatcgaag gggctaaggt ccttggaatg gaaaatcaga
     1141 taggaagtct ggaagtcggc aagcaagcag attttctggt cattcaacca caagggaaaa
     1201 ttcatctcca acctcaggaa aatatgctgt ctcacctggt ttatgcagtt aaatctagtg
     1261 atgtagatga tgtttatatc gccggagaac aggttgttaa gcaaggtcaa gtcctgacag
     1321 tagaacttta aaagaaaaat cacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt
     1381 ctttttttga ctatgctata cttatatacg gtttaaaaaa actgcctaag acagtagggg
     1441 agatcgactc ataaatatcc taccgaggac aaaacgtatc atgtaaaaag aagcgtattg
     1501 tactttcgtg tctaggtttg ggcgcgtttt tctttttgaa aaattcccca agcaaaataa
     1561 ttacggaggt gaacacacta atgagtgaag caattattgc taaaaaagcg gaactagttg
     1621 acgtagtagc tgaaaaaatg aaagctgctg catctatcgt cgttgtagac gctcgtggtt
     1681 tgacagttga gcaagataca gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata
     1741 aagttattaa aaactcaatc ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg
     1801 catctgtatt tgttggacca tctgcagtag cattttctaa tgaagatgtt atcgcaccag
     1861 cgaaaatctt gaacgacttt tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa
     1921 tcgaaggcgc tgtcgcatct aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg
     1981 aaggacttct ttctatgctc ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg
     2041 cagtcaaagc ggttgcagaa agcaaagaag acgcggctta atcttaagct acatagcgta
     2101 gcctagctac gaaaaaaact attataaaat ttaaaactta tttggaggaa ataacaatgg
     2161 cattgaacat tgaaaacatt attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg
     2221 accttgtaaa agctatcgaa gaagaatttg gtgtaactgc agctgctcct gtagctgttg
     2281 ctgcagctga tgcagctgat gctggtgctg ctaaagattc attcgacgtt gaattgacat
     2341 ctgcaggcga caaaaaagtt ggcgttatca aagttgtacg tgaaatcact ggtcttggtc
     2401 ttaaagaagc taaagaactt gttgacggtg caccagcact tgttaaagaa ggcgttgcaa
     2461 ctgcagaagc tgaagaaatc aaagctaaat tggaagaagc tggagcttca gttactctta
     2521 aataatagag tatcgcaatt tgattgcgga atccctattt accagtcttt gagagcgata
     2581 agcgattgat agaaactgat aaattgattt ggtttcctgc caaaaattct ttggcaggat
     2641 ttttgtttta ataaactttc gggggtagac actagattgt ctaccccttt ttttgtgttt
     2701 cggaggtaat tatgcaaaag gaaggacagt catcacgtca attcgtgaag agtcatctca
     2761 tgactatcat gggagttgat attgtaaaag caactcagtt agttgatgaa atggaacaag
     2821 taggtttaat ccgatttgat gaatttggaa atgttggaat tttagtcttg gagggacaat
     2881 aatgaagcgt attaccgcta atcaatatca gacatcagag cgttattata agctccccaa
     2941 acttttattt gagagtgaac ggtataagaa tatgaaactg gaggttaagg tggtttactc
     3001 agttttaaaa gatcggttgg agttgtcttt gagcaaaggt tggattgatg aggatggggc
     3061 tatttatttg atttattcca attcaaattt gatggcactt ttaggctgtt caaagtcaaa
     3121 attactctcc atgtgagttt gaagtgacat ttttagatga ttaccataaa aaacataact
     3181 acccactatt ttacgaatcc tatcttcaaa acgttatgga attccttgaa agtcaagaca
     3241 taaagaatgg ggttgatgcc tttgtagatg atcatcaaaa tctcgttttt gttttatatg
     3301 gacaaggcta tcgagccgag ggaaaagagg gaatacttac aacccaagta actgtaaaag
     3361 cttatgatga agacaagaaa ccgattaact tcgcaaattt attagattcc ttaatttatt
     3421 agattcctta atcgtgtcag aatatcaaat ggaaccgaat ctttgggagg tctcccatga
     3481 ttgatctcta tctaagtaaa aatagacaaa gaaatcaact tcttttagat ttcttccaaa
     3541 actatggcat agaggtatct tgtcattcag tttctgaaat gacaaaggac aaattaattg
     3601 agatgatgag ctattcttca gattgttttg agtttttatc tcccaattta ttacgattta
     3661 agaaccgtga caatctaaga ttaacggatt tcgttgagat aattttaaaa aatcctgaac
     3721 taaccatcag acttcctctt gcggtttcaa ataagcgagt ttatccaagt ctgaatttgg
     3781 aagaggctag agctctattg ccaagagata cgaaacaatt gatttacatg gcgcaaacac
     3841 attatttatc taactaaagg agaagcaata tggctgaacg attttgggag aacttgtcca
     3901 ttattttggc tgaaagaaat atcagctgga ttgagttaac cagaaaaatg tttgcgggag
     3961 agtttcacta tccaagtgaa ttgaatcgtt tgtatcaaaa gattcgccac tagaagatgg
     4021 aacaatgaat gcctcaaagt ccatgggtag aaaggattgt gcaggtatta gatttagatt
     4081 atgaagattt atttcggagg taactatgaa aagaataatt ccagtttata tattccaaca
     4141 agtaaatgtc ctattggtat ctctatactt attgaaattt ctttgtatcg gtgagttaac
     4201 tatactacag attctctatg gttcgtcact catttctttt ttatggatgt atggccaacg
     4261 caaacaagcg cataaggtca atatgaaatc taggatgaaa tggcttggtg ttgaatttgt
     4321 tagcctactg attataagtc tatgttttag tctgattcat gctcaaggaa gcacgaatca
     4381 agcaaactta attggccttc aacatcaaat tccttggttt tcatttttat tgttcttaat
     4441 caatgcgagt atggttgaag aatttctgta tcgagaaatt ttatggaatt tggtcagaaa
     4501 attagatatc agagttgctt tgacaagtgt tttatttgcc ttagcacatc atacaggaac
     4561 cattctagct tggtgtttgt atgtttcact tgggatgttt ttaggtatgg tgcgctataa
     4621 atctgactta tggggcagta tgggactaca tttggtgtgg aacctactag tttatagttt
     4681 gctgcttttt taaaataggt gacctgattt ttaggtaact tctaaaacta acttccagtt
     4741 ttgggagaga gatggaagtt actttgaaaa gttacggaaa cattattttc tatgtgacaa
     4801 cattgtataa ttgcttgcat tttactggtg gagagagtat aatgagggtg gataaagtac
     4861 ttaatcccaa aaatttaaat aggaggagct agctatggca gaagaagtat taaatcttca
     4921 attagtatct gttcaagttg acgaaacaga tgaagttgat ggaatgcgat ttagtacatt
     4981 cagtacaaat cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta
     5041 aattgtaaac tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg
     5101 aatgttagtg cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct
     5161 gtctagctat agatatatta agtttgataa aatttcttgg agattttgtt atggattata
     5221 atttcaattt ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca
     5281 taaagtacca agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg
     5341 tttatcaata ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt
     5401 cagagtataa cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc
     5461 gaattccatt taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg
     5521 ttagtcgagg attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt
     5581 cggttttgca aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg
     5641 ataaacgttg ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg
     5701 atgatgaaaa gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg
     5761 aacgtttacc agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat
     5821 ggcttgacaa gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta
     5881 gattttcaaa cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa
     5941 ttttaaaaga ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga
     6001 aattagcttc ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa
     6061 tatactggca cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg
     6121 ttccactaaa tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca
     6181 aagattatct attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt
     6241 ttcattctga aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg
     6301 aagatgagat tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg
     6361 tacataaagt tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag
     6421 attggtatgg tattagacaa atagctcatt atttatacat gccactagta actacatcgg
     6481 atttgacata caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt
     6541 tgggttatac aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta
     6601 aatgtccaca gattgagaat atttccagga aaaaagtttt aaaatccatg catgagatta
     6661 gaactatcga gagtgagcaa gatatacaag attttattat aaaattattg agagggttta
     6721 cgctaactta tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt
     6781 atggtctgaa ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc
     6841 aacaactagc taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac
     6901 aggttattaa tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg
     6961 gtaaaattgg tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac
     7021 tttttacaac tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg
     7081 gacaggctgg aattttactg gttggactgt attttcttag taaaggggaa attgataata
     7141 aactaggaga agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc
     7201 cagaaacttt ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt
     7261 ttggtggatt actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat
     7321 taactggtga aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg
     7381 cttataaagt agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat
     7441 atctagcaac tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt
     7501 caagcaaata cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg
     7561 tattaccagg tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg
     7621 atatcgatga taacttttca ggacaaaaga aattaattga acaactttat agatatttgt
     7681 gtgtaattga agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata
     7741 ttgcttcggg atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga
     7801 cgattcttcc tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca
     7861 gagtaatgaa atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc
     7921 ttcatcattt caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg
     7981 gtaccaattt gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga
     8041 agataatgac tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa
     8101 aattgtcttt gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag
     8161 gatttcatct gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt
     8221 aattggaata aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga
     8281 aagttcgttc tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag
     8341 gccaagtaag ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga
     8401 aaacgaatgc tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa
     8461 gtcagtagac tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt
     8521 tagacactct tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac
     8581 agaaaaagca cagtactttc ttgggatctc ttcaactgaa gaaaagaaag atcaactaat
     8641 tctaagggaa acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc
     8701 tagaagagtt cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc
     8761 cgtcttttat cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat
     8821 aacatttgat gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt
     8881 tttagagagg agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt
     8941 aaaaactgaa tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt
     9001 gatttggact aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag
     9061 tgaagatacg gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta
     9121 tggtaagaat gataattctg atttagatga aattatgtta tctataatag atgcaggttt
     9181 tatttatgct atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc
     9241 aggaaaggcg ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt
     9301 aagggaaaat gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg
     9361 aataattgct ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt
     9421 ttcgccattt ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac
     9481 cgaaatagca gagtggggag atattagagc ccctgaagta aaggcttata taaagtcata
     9541 ttcgcctatg caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt
     9601 gggtgagaaa gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa
     9661 ttctattgga gttaaaagtt tgttgtattt aaacaaagca gctgatcatg gaggttttac
     9721 tccatcagat gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag
     9781 gaataactta tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt
     9841 ttaatatttc tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt
     9901 agtttaaatc agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg
     9961 ggttggtcag caatctatat atcaaattac tatttagaag taacggaagc atcaataacg
    10021 aaagatataa atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa
    10081 atggttaaag attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa
    10141 gaaaactatt ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct
    10201 ctaagtttta tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca
    10261 ttgccgatta tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac
    10321 tctaacagca atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca
    10381 ttaaaatctt attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa
    10441 ctagaagatt ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta
    10501 ctaatttcag gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat
    10561 cataaaagcc tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta
    10621 gggccgatac aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg
    10681 aaaccgtttt taaaatactt aagtggagag aagaatttta tagacgctga acatgataat
    10741 aacggactgt atacttcatc aatagatgag atacacatga aagatgttgt atattctatt
    10801 acaccagaaa ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta
    10861 acaggaactt ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag
    10921 ccacaaaaag gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca
    10981 acagttgatc agacaccata tatttttgac actactattc gtgagaacgt aactttattt
    11041 caaaatgaat atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat
    11101 gaagaattag aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg
    11161 tctggaggtc aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg
    11221 tacttatttg acgaaatatc agctaattta gataatgata attcaaattc catacatgat
    11281 attctgttca atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac
    11341 aagagataca ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg
    11401 gaggcaagaa tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt
    11461 tactaggaaa taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag
    11521 gagaatttat aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta
    11581 tttctagttt gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata
    11641 aaatcagaaa tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag
    11701 catacaattt aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat
    11761 cacaaaaaaa attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg
    11821 ctggcttaaa cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag
    11881 ctagagcggt tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag
    11941 acagcgtttc tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt
    12001 gtgtaattat ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat
    12061 tgaaagatgg aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa
    12121 tcttagaggt acaatcaact acggaggaat agagatgctc agattagttt actatcaatt
    12181 tttacataat aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt
    12241 tatgggattg gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg
    12301 acttccagat cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt
    12361 ctttgtgctc tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact
    12421 tctggaggtg ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt
    12481 tatcatttcc tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa
    12541 ttattattat tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca
    12601 aacgagtgct gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag
    12661 tggttgcttt tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt
    12721 tctttcaata gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc
    12781 tttgcaacaa attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat
    12841 tgtaatctta gatattgtga ttatttatca attatctcct tttatacagt catgttttat
    12901 caaactactt tctataatta tatttagaaa taattttatg ttcattgttt cgaagtggaa
    12961 tttactatat cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt
    13021 actaatttct agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag
    13081 tgatttagag ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact
    13141 tgcaaatatt atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca
    13201 atttgaaatt ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat
    13261 atttttgaca tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt
    13321 aatatactac tctttagagg atattttaat agatgatatg aatcttttgg ggttaatact
//
LOCUS       DBERYS010000047        12983 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.047, whole genome shotgun sequence.
ACCESSION   DBERYS010000047 DBERYS010000000
VERSION     DBERYS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.047"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(159..1361)
                     /gene="rpsA"
                     /locus_tag="ACL8LA_001686"
     CDS             complement(159..1361)
                     /gene="rpsA"
                     /locus_tag="ACL8LA_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGS1813411.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1523..1594)
                     /locus_tag="ACL8LA_001687"
     tRNA            complement(1523..1594)
                     /locus_tag="ACL8LA_001687"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Gln,seq:ttg)
     gene            complement(1604..1684)
                     /locus_tag="ACL8LA_001688"
     tRNA            complement(1604..1684)
                     /locus_tag="ACL8LA_001688"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1648..1650),aa:Tyr,seq:gta)
     gene            complement(1734..1964)
                     /locus_tag="ACL8LA_001689"
     CDS             complement(1734..1964)
                     /locus_tag="ACL8LA_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGS1813412.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2132..2776)
                     /gene="pcp"
                     /locus_tag="ACL8LA_001690"
     CDS             complement(2132..2776)
                     /gene="pcp"
                     /locus_tag="ACL8LA_001690"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS1813413.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2791..3714)
                     /locus_tag="ACL8LA_001691"
     CDS             complement(2791..3714)
                     /locus_tag="ACL8LA_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGS1813414.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3711..4400)
                     /locus_tag="ACL8LA_001692"
     CDS             complement(3711..4400)
                     /locus_tag="ACL8LA_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGS1813415.1"
                     /translation="MEWIRLIGIAIIVVGFIFKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4465..5169)
                     /locus_tag="ACL8LA_001693"
     CDS             complement(4465..5169)
                     /locus_tag="ACL8LA_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1813416.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(5225..6247)
                     /locus_tag="ACL8LA_001694"
     CDS             complement(5225..6247)
                     /locus_tag="ACL8LA_001694"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGS1813417.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6379..8850)
                     /gene="parC"
                     /locus_tag="ACL8LA_001695"
     CDS             complement(6379..8850)
                     /gene="parC"
                     /locus_tag="ACL8LA_001695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGS1813418.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9051..9255)
                     /locus_tag="ACL8LA_001696"
                     /pseudo
     CDS             complement(9051..9255)
                     /locus_tag="ACL8LA_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(9270..11213)
                     /gene="parE"
                     /locus_tag="ACL8LA_001697"
     CDS             complement(9270..11213)
                     /gene="parE"
                     /locus_tag="ACL8LA_001697"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGS1813419.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11349..11990
                     /gene="plsY"
                     /locus_tag="ACL8LA_001698"
     CDS             11349..11990
                     /gene="plsY"
                     /locus_tag="ACL8LA_001698"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGS1813420.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12033..12983)
                     /locus_tag="ACL8LA_001699"
                     /pseudo
     CDS             complement(12033..12983)
                     /locus_tag="ACL8LA_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3725 a         2919 c         2278 g         4061 t
ORIGIN      
        1 acttcaaaaa acggattttt atcgctctga acatcaaaaa agaaaggacg aaatttgtcc
       61 tttctcaacc ttagctgact tcaacccact acagttgaca aagagccaga aaaatccctt
      121 gtttagtaaa caaaggattt tgtgaatttt caatttgatt aaagttcgat atcaccaaac
      181 aaatcagcca ttgaaaatcc tgtttgtgtt tctggaagtt cgaaatcacg cttttcttga
      241 cgtcttggac gacgtggacg agcagcacgt ttttcttctt tttgtccttc ttcttgggct
      301 ggacgttctt caagagcttt aatagaaagt gatacgcgtt ctgcatctgc gttaacttca
      361 agaactttaa cttgaacttc ttgaccaact ttaagagctt cttttggatt ttcaatccgt
      421 ttgtgtgaaa tttgtgatac gtgaacaagt ccatcgatac ctggcaatac ttcaacaaat
      481 gcaccgaagt cagtcaaacg tttaactgtt ccttctacta catcaccttt agccaatttt
      541 tgctcaacgc catcccatgg tcctggtact gttgctttaa gtgaaagtga tacacgtcct
      601 tcttcttcgt taagatcaag gattttcact tcaatttctt caccaacagt tacaactgat
      661 tttggtgata cgttacgttc atgtgacaat tcagtcaagt gaaccaatcc gtcaacacca
      721 ccaaggtcga tgaaagcgcc gaagcttgtg atacgagcaa ctttaccagt tacaacatca
      781 ccaacagcca atttaccgaa tacttcagcg cgagctgctg cagtagctgc ttcaacaact
      841 tcacgacgtg aaaggatgaa gcggttttct ttagcgttaa cttctttgat tttagtatca
      901 aattcttgac ctacaaaacg ctcagcgtta cgtacgaaac gagtatccaa cattgaagct
      961 gggataaatc cacgaacacc ttcaaattct actgaaagtc cacctttaac ggcacgcgtt
     1021 cctttaacag taacaacttc ttcttcgcga ccaacaagtt tgtcccatgc tttgcgagct
     1081 tcaaggcgtt ttttagatac aaggtatgta actgtatcag tatctttacc aactacttga
     1141 cgaagtacaa gaacatccaa tacttctcct actttaacaa agtcattgat atctgcatca
     1201 cgatcgtttg tcaattcgcg aagagtcaag acaccttcaa caccagttcc agagattgca
     1261 acgttagctt gagtcgcatc aactgtcaat acttcagcac taacaacatc accagtctca
     1321 acttgactaa cgctatttag caaatcttca aattcgttca tctaaaaaat cctccaacaa
     1381 tcaagttttt cttaaacttg acatacttat aatttttttc ctaagcaccc gcaaggacat
     1441 gttcatatca ttcacgtctt tcaattataa aaataaaata ccctaagata ttttgctttt
     1501 tatcttgatt attacctaag aactggggta gctggattcg aaccaacgca tgagggagtc
     1561 aaagtccctt gccttaccgc ttggctatac cccattaatg aaatggagag agagggattc
     1621 gaacccccga acccgaagga gcggatttac agtccgccgc gtttagcctc ttcgctatct
     1681 ctcctacaat caacatggac tattatatca tgaaaatttc aaaaaaacaa gacttatttt
     1741 gctaaattat aattttcaat caaaatttcc acagcttttt ccaatttttt ataaaaacta
     1801 tcgacattcc cattctttat aatggcaaat tgtccgtcta attcggcaat ttctccgata
     1861 acttttttac cgatagtcaa tgtataacct tcaaaactgt ctttaccaac attaactttg
     1921 gcatccgcta cttgaatttc gattttttta tctttcttgc tcatttcata cctctcttca
     1981 ctttttctat tttacaagaa aatcctaaaa ctagcaagaa aaaactctat atcaggcttc
     2041 tctgatatag agttttatgt aaacaacctc aatcgattta gcatagctaa tagttagtaa
     2101 acaagcataa ttgactatcc tttttaggtt atcagtgagt ctcaccgcct actaactgca
     2161 aatccttatc tttgtagtct acaatagctt caatagctac ctctatgcct cttacaatat
     2221 cacatagact cattcctgca gtattcggct tatttactac ttgttccatc atatatggaa
     2281 tatgcatgaa acctgctctc atattaggga attttttatc cactaaataa agagcttggt
     2341 acatcaaatg attgcaaaca aaggttcctg cactattgga tacaactgcc ggaagtccct
     2401 gttttttgat agcttgtacc atcgctttga taggtaaact actaaaatag gccgatgctc
     2461 catcaatacg aatcggtgta tcaattggtt gattgccttc gttatcaggt atgcgagcat
     2521 catcttgatt aatagccact cgttcaggtg ttaagccggt cctgccgcct gcttgtccaa
     2581 tacaaagtac agcatctggt tgatatcgta atatttctgc ctctaaaact tctgacgact
     2641 tataaaaaac cgttggaatt tctacccagc gaacttcagc cccattaatc tcagatggta
     2701 ataattttac agcctccaaa gctggattaa tcttttcacc tccaaaagga ttaaaacctg
     2761 taaccaatat tttcatttta ttttccttta ctaaaatgcg agaaagtaca ttaagaatat
     2821 gtgaataaca atcattacta gagcaacacc tgcttgagcc tttataacgc cattctgatc
     2881 tttcatatcc atcaatgctg ctggtagagc gttaaaatta gcagccattg gggtcaataa
     2941 ggtcccacaa taacctgctg tcatggcaag agcaccagcc acaattggat tagctcccag
     3001 agcaaataca aagggaactc caacacctgc tgtaataacg gtgaatgctg caaaagcatt
     3061 tcccataatc attgtgaata gaaccattcc aagaacatag gccaaaactc ctataaagcg
     3121 actatctgaa ggaacaatac cgctaatcag atgagagata acatcaccaa cacctgctac
     3181 agtaaaaata gcccccaaag cccctaataa ttgaggaaca ataccacttg ttgaaacttg
     3241 ctgagtcatt cgattatttt ctgataacag actcttaggg tgactattgg taatcacaag
     3301 aacagaaatt gtagcaaaca aggcggcaag gctaatcgaa atcttgctaa attctggaat
     3361 catttgcgct aagaccaacg caagtattgc catcagcata actggaataa aaattttatt
     3421 tttcaacctg ttagattcaa tattggcttt catttcatct aaggatggca aggttccgat
     3481 acggacttgc ttaaacaatg ttaacagcga taataggatt acaataatac caatactcat
     3541 atttggcata taggaaccac ctataaacgt aatagacaat agagtccaaa atgcagatgt
     3601 cccaagtcga actgggtttg ttttatcttt ataactacaa taggctgtat ggagaaattg
     3661 acaaccaatc acaatatagg tcaactctaa tagttgcttt gccaactctg tcatttttgt
     3721 tctcctcccc tagtcttttt tgatatcaat tttttatcaa ataaataatt gtaaatcccc
     3781 actacaataa gtgttataac agcaacaata atagatgtag aagcaatccc tgcataattg
     3841 ctttcatagc ctaactgatc taatgttccc cctatcaaga ggactccccc agcacctaca
     3901 aacgtatttt gagcaaagaa atttccaaaa ttttcattcg cagccgcacg cgcttttatt
     3961 gtctcatctt caacctctgt taactttcta cctaattgag actctgcagc tgcttctccc
     4021 ataggttgaa ccaaaggtct gacaaactga gggtgtcctc ctagacgaat tgaaaagaaa
     4081 ccagctaact ctcgaataaa aaaataaact gtatagaagt ttccaactgt cagaccttta
     4141 atctttcgaa tcaaatcgat tgatcgttgc ttgagtccaa aggtttctga cagccccaca
     4201 agaggcaagg taaccataaa aatcgtgagc actcgctgat tgctaaattc ttttcccaaa
     4261 atctccaaaa attcaacgag agaaacacct gaaactaaag ctgtaaccaa accagctaag
     4321 actactgttg caattgtatc aaatttaaaa ataaaaccca caacaatgat tgctattcct
     4381 attaatctaa tccactccat atcaaactcc tttatattca aaatgacagt attttaaaaa
     4441 ttttatcaag atcaatacca ttccttattt aatgtatttt tctagttctt tttggtattt
     4501 gctattggat tccaattttt ctttttgcca ttttttaaaa acctcgttat attcttttgt
     4561 tgtaacaata tctttttgca atttcattcc tttaaagata tatggttccc ccttaatacc
     4621 aacttgtgag tatggttttg agaatggtac tacgttactt acaactggag aaccaccaga
     4681 tgaagctgtt ggcatcaata atgaactatc tgtcgaccaa gcttgagctt tggcatattt
     4741 ttcatatctc ttctctaggt cagtggtctc agaaacagca tcttctaaca atttcttata
     4801 tttatccaaa ccaggtttag ctacaacatc cttatctttt cctttcgtaa taccaaggtg
     4861 tttcatggca gaaccagatt ttggatccat aatattcaag taagacgctg gatcttgata
     4921 gcttggagcc catcctgtac tattcaaatc atagtctttt tgagaaggag caacattgcc
     4981 gtatttatca ttttccatca aaccatcaat aacatttcca ataacgtctg tcctcgatgt
     5041 tcgagtcgct atactgtagc ccaatgatgc tggatctact gcatagacat aagaaaatgt
     5101 tgtcggtgca tctgcttctt tatcagtttt tccacaagcc actaaaatag ctgacgtgct
     5161 caggaccact cctgctgtta agagccactt tttctatttc ataaaaaatc tcctttggtt
     5221 tattttaatc tacttttaca atccaacctt ctggcgcttc aatatcgcca aactgaatac
     5281 ccgtcaattc attatataat ttacgcgtca caggacctac ttctgtttca ctatagaata
     5341 catggaaatc atcaccatgt tgaatacctc caattggaga aataaccgct gctgtaccac
     5401 aggcacctgc ctctacaaaa cggtcaagat tatcaattgg aacatcaccc tcaataggag
     5461 ttaatcccaa gcgatgttct gccaaataaa gcaaggaata cttggtaata gatggcaaga
     5521 tagatggact caatggtgtt acaaattcat tatcagctgt aattccaaag aagttagctg
     5581 atccgacttc ttcaatcttt gtatgagttg atgggtccag atagataaca tctgagaaat
     5641 gacgtgactt ggccattttt cctggtaaga gacttgcagc atagtttcca ccaaccttag
     5701 ccgcacctgt accatttggt gctgcacggt cgtactcatc ctgaatcaag aagttggttg
     5761 ggaccaaacc acctttaaag taatttccaa ctggcatagc aaagatggtg aaaatgtact
     5821 cttctgccgg ttttaccccg ataatatctc cgacaccaat caaaagaggg cgaagatata
     5881 aggttccacc tgttccgtat ggtggtacgt attcttcatt cgcacggaca actgctttac
     5941 aagcttctac aaacatgtct gtcggaactt gtggcatcaa gagacggtca catgtacgtt
     6001 gcagacgttt agcattttca tcaggacgga acagttgaac actgccatcc ttagtacgat
     6061 aagctttcaa accttcaaat gcttgttgtc catagtgaag acttggagaa gactctgaaa
     6121 tatgcaaagt tgcatcctct gtaagctctc cttgatccca ttgtccattt ttgaaatgag
     6181 caagatagcg ataaggtaat ttcatatagg aaaaaccgag gttttcccaa tcaatcgtta
     6241 ctgtcatatt ccactcctta ttctaaaaac ctatttctta tattctacac tatttttcta
     6301 aaatagcaag tatattttgt aattttcaga aaatttctcc aataaaaacc agctcttaga
     6361 actgattctt catttcactt atttatcttc agtaactact tcctgaagat aagcgtcaaa
     6421 aacttcttca tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc gttctgacaa
     6481 gttcaagtct tgcaatcggc tttcatagat tgttccttta ttggattgga caagcagagt
     6541 ttggtcgttc acatccactt ccgtactgaa gaaatcgcca acaaatcctt gctctgcaac
     6601 tgctcctgcc aagaagacac gatgcggttt gtttttcaac tcacgcaaga cttgtaatcc
     6661 tcgtttggca cggctggttg ctagaatttc ctcaatggaa acacgtttca agcttccacg
     6721 ctgggtcaag aggtagaagg acgaagtatt acagataaag ccagattgga ggacatcatc
     6781 ttctttcaaa ttcatagcct tgacacctgc tgccttagca ccgacaaccg gaacctcttc
     6841 gatattgaaa cgcagggcat aaccattttg actaaccaag acaacatcat ctagtttaat
     6901 cggagccact gctacaatct gatctgtatc gtctttgagc ttagcatact tgacagactt
     6961 agatctatag gtccgccatg gagtgaattc ttttcgctct acccgtttga tttgaccaag
     7021 gcgagtcact gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt ccacataaag
     7081 gatttcttca ttcgtttcaa agtttgtgat ggtttggctc agatgctctc cgatgtcctt
     7141 ccaacgaata tctgccaact catggattgg tctgtagatg acatttccaa gacttgtgaa
     7201 catcaagagg tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt catcatcacg
     7261 cttgccaatt tcttccaagg tggaagccgc aaaggaacgt ggactggtac gcttgatgta
     7321 acctgccttg gtcacgctga cgtaggtatc ttcctcagcg ataagactag ctgtatcaat
     7381 ctcaattgct ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa atttcttctt
     7441 gacctcacga agttctttct tcatgagatt gtacatagtc ctttcatcac cgataatagc
     7501 cgccagcata gcaatcttct cacgaagctc tgcttcttct tcctgcaaga caaccacatc
     7561 ggtattggtc aaacggtaca gttgcaaagt tacgatagcc tcagcctgtt cttccgtaaa
     7621 atcatagcta actttgaggt tttccttggc gtccgcctta ttctcagaag cacggataag
     7681 agcaatgact tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga tatggagacg
     7741 tttctcagcc ttttctttgt caaagcgtga acgcgccaaa atcacttctc gacggtgagc
     7801 gatgtagcta gacaggattg gaacaatccc aacctgacga ggtgtgaaat tgtcaatcgc
     7861 caccatatta aagttgtagt tgatttgtag gtcggtgtac ttaaataagt agttgagaac
     7921 aagctcagta ttagcgtctt tcttaagttc gatagcgata cgaagaccat cacggtcaga
     7981 ctcatcacga acctcagcaa tcccagctac cttgttatta acacgaacat catcgatttt
     8041 cttgactaga ttggccttat tgatttcata aggaatctca gtaataacga tttgttcctt
     8101 accacctttt agcttttcaa tttcagtctt ggaacgaaca accacgcgcc ctttcccagt
     8161 ttcataagct ttcttgattt catcacgacc ctgaataata gcccctgtag ggaagtctgg
     8221 tccaggcaag aattccatga gtttatcaat ctttgcagtt gggtggtcaa tcatgtaaac
     8281 tgcagcatct atgacctcag ctaaattatg gggaggaatg tctgtggcat aaccagccga
     8341 aatcccagtc gaaccattga ccaagaggtt tggaaaggct gctggcaaga ccgttggttc
     8401 tttctccgta tcgtcaaagt tccatgcaaa aggaactgta tttttctcga tatcctgaag
     8461 aaggtaacct gcaatttcag acaaacgtgc ctcagtataa cgcatagccg caggaggatc
     8521 tccgtccata gaaccgttat taccgtgcat ttcaactaga atctcacgat ttttccagtt
     8581 ctgtgacata cgaaccatgg catcatagat agaagaatcc ccgtgtgggt ggaaattccc
     8641 catgatgttc ccgactgact tggccgactt acggtagctc ttgtcaaaag tattgctatc
     8701 cttattcata gaataaagaa tacggcgctg aaccggcttc aacccatcac gaatatctgg
     8761 caaagcccgg tcttgaataa tgtacttgga gtagcgacca aagcgctctc ccatgatgtc
     8821 ctccagggac atgttttgaa tgttagacat aagatacaaa gcccataaaa taccaagtga
     8881 aaatagaaaa ttcttgaagt aagcaaactc acaagagaat ttatcttttt cacacagtat
     8941 ctagggcgtg ttcaactcct ttcaaagaat gtagagtagg tttttatgca gtaaaagata
     9001 ttttacggga attcctcccg tgttcagtta cgataagtaa ccaaactatc ctgtttgtat
     9061 ttttcaatat gaaaatctgg ttttccaaaa ttagtcttag tttgtgtctt agccgctccc
     9121 ttaagcgcct ctttgagata agcactcata gcagattctt cattaataat cctgcaattt
     9181 tttcaaacca agattttcaa actgcttttt cacatagtca ttcacatccg actctaattt
     9241 ccagtttact aacatattat tttctttcat taaaacactg tcgcttcttc tagcgtaaac
     9301 ttgacattat cttcaatcca tttacggcgt ggttctacct tatctcccat gagaacattg
     9361 acgcggcgtt cggcgcgcgc taaatcttca attgtgacac ggatgagggt acgtgtttct
     9421 gggttcatgg ttgtttccca gagctggtcc gcattcatct caccaagtcc tttgtatcgt
     9481 tggagggtag cgcctttacc gaactgttta cggagttctt ctagttctcc gtccgtccaa
     9541 gcgtaggcca cttcttcttt cttgccttta cctttggaca tcttataaag aggtgggagg
     9601 gcaatataga catgacctgc ctcgactagc ggacgcatgt aacggtagaa aaatgtcaag
     9661 agcaaggtct ggatatgggc accgtcggta tccgcatcgg tcatgataat gatcttatca
     9721 tagttggcat cttcaataga gaagtctgct ccaacacccg caccaatggt ataaatcatg
     9781 gtattaatct cttcattttt gaggatatcc gccatcttgg ccttggctgt attgacaacc
     9841 ttaccacgaa gaggtagaat agcctggaac ttgcggtcac gaccttgttt ggcagaacca
     9901 ccggcagagt ccccctcaac tagatagagt tcattcttag caggattctt agattgggct
     9961 ggggtcaatt tcccagacaa caagccctta tctttcttgt ttttcttccc atttcggctc
    10021 tcatcacgcg ccttacgtgc tgcttcacga gcatcacggg ccttgatagc cttgcggatg
    10081 aggttagaag ctaattcccc attttccata aggaaaaagg tcaacttatc agccactatt
    10141 ccatccacaa ctgggcgagc tagggggctt cctagtttat ccttggtctg tccttcaaac
    10201 tgcaagtgtt cttcaggaac taagatagaa agaacgaccg ctagtccctc acgatagtct
    10261 gaaccttcaa ggtttttatc tttttccttg agaagacctg ttttacgtgc atagtcattc
    10321 atgaccttgg taatggcaga cttgaatcct gtctcgtgcg ttccaccgtc cttggtgcga
    10381 acgttattga caaaggatag aatgttatct gagaatccgt cattgtactg gagggctact
    10441 tccacttgaa aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa gatttcctta
    10501 tcttcgttga gataagaaac aaaatcttgt actccattct catagtggaa ctcaatcgct
    10561 tcatctgttc gcttgtccgt taaagacaag gtcacatttt tcaagagaaa ggctgattca
    10621 ttaaggcgct ctgaaatggt attgtacttg aaatctgtcg tagaaaagat agtcgcgtca
    10681 ggcataaaag taactttggt gcctgtttta gacttgggtg ctgtaccgat tttcttcaaa
    10741 gtcgtgacag gttttccacc attttcaaaa cgttgcttgt aaactgcgcc atcacgggta
    10801 atttcaactt ctaaccagct agaaagggcg ttaacaacgg aagaacccac tccgtgaagt
    10861 ccacctgatg tcttatagcc accttgaccg aatttccctc cggcatgaag aatggtaaag
    10921 ataacctcaa cagttggaat tcccatagcg tgcatacctg tcggcatccc acgtccatgg
    10981 tcttgaaccg ttagactacc gtctttattg atagttacat caatacgatc accaaaccca
    11041 gacaaggctt catcgactgc attatcaacg atttcccaaa ctaggtgatg aagaccagcg
    11101 ccatcggtcg atccaatata catccctgga cgttttcgga ccgcatccaa cccttctagc
    11161 acctgaatag catcatcatt ataattgtta atattgattt ccttttttga cacaaggaac
    11221 ctcctattcg ttcatcttta ctattctaca ggttttccaa ggattttgca aaatttttct
    11281 ttctccgatg tgacaatttc agcagagatt ctctgctttt ctttcccaat tcatgatata
    11341 ataggagtat gattacaata gttttattaa tcctagccta tctgctgggt tcgattccat
    11401 ctggtctctg gattggacaa gtattctttc aaatcaatct acgcgagcat ggttctggta
    11461 acactggaac gaccaacacc ttccgcattt taggtaagaa agctggtatg gcaacctttg
    11521 tgattgactt tttcaaagga accctagcaa cgctgcttcc gattattttt catctacaag
    11581 gcgtttctcc tctcatcttt ggacttttgg ctgttatcgg ccataccttc cctatctttg
    11641 caggatttaa aggtggtaag gctgtcgcaa ccagtgctgg agtgattttc ggatttgcgc
    11701 ctatcttctg tctctacctt gcgattatct tctttggagc tctctatctt ggcagtatga
    11761 tttcactgtc tagtgtcaca gcatcgatcg cggctgttat cggggttctg ctctttccac
    11821 tttttggttt tatcctgagt aactatgact ctctcttcat cgctattatc ttagcacttg
    11881 ctagtttgat tatcattcgt cataaggaca atatagctcg tatcaaaaat aaaactgaaa
    11941 atttggtccc ttggggattg aacctaaccc atcaagatcc taaaaaataa aacgccagtt
    12001 ctgtactgac cccaaaaagt tagacaatta atttatccga aggatttagt tctgtattgc
    12061 acagggctaa gtccttttag ttttacctta attcgtttat tgttgtagta atcaatatag
    12121 tctacaatgg cttgttccaa ttgcttaagc gactgaaacg acttctcata accgtaaaac
    12181 atttccgatt tcagaatccc aaagaaggac tccatcatac cattgtctgg gctgttaccc
    12241 ttacgtgaca tggatgcttg aattccctta ccctcaagaa actgatgata agaatcgtgt
    12301 tgatattgcc agccttggtc actatggaga atcgtattct cgtagtgctt ctctgtgaat
    12361 gcctgttcca acattgtttg tacttgttct aagttgggtg aagttgaaag attataggcg
    12421 ataatttcgc tgttaaaacc atctaaaact ggtgataagt aaagcttttg agtacttact
    12481 ggaatggcaa attcagtcac atctgtgtag cactgttcca ttgttttaga gccttcaaat
    12541 tggccttgaa tgagattctc tgctttctta ccaacgtctc ctttatgaga agaatatttt
    12601 cgtttctgtc gcattttagc ttgtaaattg agtactttca tcaagccttg aactctttta
    12661 tgatttacca gataaccacg atttcttagt tctaaataaa tccgacgata agcataattt
    12721 cccttgtgtt cgataaaaat ggattgaatt tcagctttaa gctcttggtc cttatctggt
    12781 ttgtctagct gtttcaagtg atagtagtag gtcgaacgag ctagtttaat ggcttttaga
    12841 agaatatcta acgaaaactc agtcattaat tcttgaacaa tttctgtctt tcttctttct
    12901 ctttttcctc cttcaatcgg agttctctta acttttttag gatggcattc tccgctctca
    12961 ggtactcatt ttctgcttga aga
//
LOCUS       DBERYS010000048        12908 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.048, whole genome shotgun sequence.
ACCESSION   DBERYS010000048 DBERYS010000000
VERSION     DBERYS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.048"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(98..610)
                     /locus_tag="ACL8LA_001700"
     CDS             complement(98..610)
                     /locus_tag="ACL8LA_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGS1813421.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(635..1210)
                     /locus_tag="ACL8LA_001701"
     CDS             complement(635..1210)
                     /locus_tag="ACL8LA_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGS1813422.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            complement(1220..1615)
                     /locus_tag="ACL8LA_001702"
     CDS             complement(1220..1615)
                     /locus_tag="ACL8LA_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HGS1813423.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            complement(2091..2900)
                     /locus_tag="ACL8LA_001703"
     CDS             complement(2091..2900)
                     /locus_tag="ACL8LA_001703"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS1813424.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(2897..3457)
                     /locus_tag="ACL8LA_001704"
                     /pseudo
     CDS             complement(2897..3457)
                     /locus_tag="ACL8LA_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(3459..4670)
                     /locus_tag="ACL8LA_001705"
     CDS             complement(3459..4670)
                     /locus_tag="ACL8LA_001705"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGS1813425.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="ACL8LA_001706"
     CDS             complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="ACL8LA_001706"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGS1813426.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(5665..6504)
                     /locus_tag="ACL8LA_001707"
     CDS             complement(5665..6504)
                     /locus_tag="ACL8LA_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGS1813427.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(6518..7339)
                     /locus_tag="ACL8LA_001708"
     CDS             complement(6518..7339)
                     /locus_tag="ACL8LA_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HGS1813428.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(7476..8333)
                     /locus_tag="ACL8LA_001709"
     CDS             complement(7476..8333)
                     /locus_tag="ACL8LA_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGS1813429.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(8487..9221)
                     /locus_tag="ACL8LA_001710"
     CDS             complement(8487..9221)
                     /locus_tag="ACL8LA_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1813430.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(9221..9904)
                     /locus_tag="ACL8LA_001711"
     CDS             complement(9221..9904)
                     /locus_tag="ACL8LA_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1813431.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            10094..10324
                     /locus_tag="ACL8LA_001712"
     CDS             10094..10324
                     /locus_tag="ACL8LA_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGS1813432.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            10553..12811
                     /locus_tag="ACL8LA_001713"
     CDS             10553..12811
                     /locus_tag="ACL8LA_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1813433.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         3865 a         2915 c         2318 g         3810 t
ORIGIN      
        1 aattttgttc tttcacagag agctacctga gttctattca agctcagata gtactttctt
       61 ataaactaga caaactaact gtcattgtac catcagatta caagacatca tcgtcactca
      121 ccttggaatt caatgtcgta ccccaatggg taattttacg gtggggttga gctaaaattg
      181 gtctgttttc atagattgtt tgccatctat tccatagtag gcccgtcttt ttctcaatct
      241 taactcgcag atttctcata ttttctttga ttgggaagtt gaggacaaaa cctgcagtct
      301 ggttgcaacc gtttccttcc caagaatgac tacgaacaac ttggtttcca tctttatcta
      361 ctggaacttc ttcccaagtt atggagtagc gggcaatgta agctccactg tgttgaattg
      421 tcaatgtttt atctttcaca ggagtctgac tgattggttg aactggctta gaaacttgtg
      481 tcgccgtttc agcattcgta gctataaagc ggaaaacttc aacttctgca agactgaggg
      541 cattatagcc ttctagccca atccgaacat agcgcgcttt tttatggtta atcgtaattt
      601 gtgctgacac atctttagga gatattatac gttttcactc aatttcttta ccagaactgt
      661 ctaaaagaat gacatcaaag tttgccaatc tatcctgggc agtgtctgtt cggttgtaaa
      721 tattgatttg gcgaatggtt tcttctttag ccaaatctac ttgccaccaa ggcttagatt
      781 ggaagtttgt atgagtgaca gaattgtgac catagttacc atcgactttg ccatccacag
      841 ctcttctagc atctcctccg aaagctgtcg aactttgagt aacctgtttt cctaaggcaa
      901 tattttccat tggttcagcg cttggttgac tagaagattg aggtagagcc tgtttcttaa
      961 agaatcgcac tttatccaat ttcggatcac gataaccatg tttatccaag gtagcttgtt
     1021 taccaatatt aaagttagga atatcattca ttggactttc aaaatagcca cgagaacggt
     1081 gtttaaagcg tgaattacct gtaaatgtaa caatatcacg attattatga atcaagggag
     1141 tcacaacatt gtcattatca accacataat gcttaatttt tccactaaca gctaatttac
     1201 gactttccaa accaacatct cagaaaccat tcttagcatc ccaaacagtt ctggaagtaa
     1261 tcactttagg agcactgaaa gttgtcactt tcctcaatac atggttataa aaatcaggat
     1321 atttttggta atcttcaacc gctgcaatct gagctaggta gcctccaaga gaatgacctg
     1381 tcatatacaa cttagtaata cttatatcct tggctaattc cccaacaacc ttgcggatgt
     1441 catccgcttg agctggctct gttatctcta cttttgactc ttcccttttg ttttcaagaa
     1501 cttcacttgc ttccggaagc gtttgaggag ctagtcttgg ctcctctcta agaggagttt
     1561 ctagttttgg agcatcattt tctgactctt tcaattcctc cttagaatct agcacaccta
     1621 ttaccacttc agttgttaca tcttgcttta cttcattggc tgaaattcca gctggagcta
     1681 gaaatcaaag ccgactacaa cagatacaac tccgatactt aattttttat gccaaagcgc
     1741 ataattcgtt gaccaatttt cattatttct tatctttctt ttcgtttatt tacaagcaga
     1801 ctcaagcgaa atctcattgt gctatatatg ataagactga gaaattcaga aagtcaattc
     1861 attctcatct ctccttatca tactagtctg cttggctagg aagctaatgc tagtatccta
     1921 gaatcttgtc tttcacaatg ctggctatca gatcagacac acctattctt ccgcttcttt
     1981 cagttataag agcaggaaga agctcatagt attcctctgt ttcttcttta atacggatag
     2041 gcagtcaatc ctgaaaagac agaggcactc ccccttttaa atcttttttc ttacaattca
     2101 gcaagaatcg ctttaagcaa gcctttaaaa tcacctttaa cacgttcagt cacttctaca
     2161 acttcttcgt gattgagttc ttcttggaaa ccagccgcaa agttagtgat acatgaaatt
     2221 cccagaactt tcaagccaga gtgggctgcc acgataactt caggaaccgt agacatacca
     2281 actgcatctg ctcccagtgt cttataggaa cgaatttctg ctggtgtttc ataagtcgga
     2341 ccagtaactc cgatatagac accttcatca agcttgatat taagtttttt agccacttca
     2401 tgggcagtag cacggtattc tggtgtgtag gccctagaca tatctgggaa acgtgggcca
     2461 aagtcatcca agttttcacc catcaatgga ttttgccccg tcatgttgat atggtctgag
     2521 atagccatca aggtaccagg accaaatccg ataccgccag ctgcattggt tacaataaca
     2581 ccttcacatc caagaacttt catcacacga actgggaaag tcaccacttc cagaggattc
     2641 ccttcataga aatggaaacg cccttgaaga gccaagacct tgcgacctgc cagttcacca
     2701 tataccaatt taccagcatg accgactact gttgaacggc cccagtttgg aatctcagca
     2761 tagtctacta caactggatt ttcgatttct tctgccaatt ctccaagtcc tgacccaagg
     2821 attagaccga actcaggggc tgcaattccc ttttctttca ggaaagtagc agtttcatgg
     2881 attttgttta aaaatgtcat tgtgtgtctc ctttattatt tttttagcat ttgaccaatt
     2941 ttgtcaaagg ttttagcatt acgaatagta atgtggcggt agaaaggcac ctcagcaagt
     3001 acttatgata ggcagtctta gaataggatt cttcagaaaa tttcccctag aaaatcccaa
     3061 gttttccaaa ataaagcact tcatctttca gctctaaact ttcaactgtc gcgatgactt
     3121 ggtccacatc caaaccctca gtgtaaaaga gaaaatcttt tcgtgccaaa tctctgctcc
     3181 accaagctgg tagattttca agttccgcct caaagtcctc tagactcagt aaagaaaagc
     3241 tctgaataaa tggataatgg actgcaaaga aagtctctag cttttcaacc aattgggctt
     3301 tggaatctat cgaagtaaag aaaatattgc cactgttgat gtagctctca accttttcca
     3361 gtcccaagtt tgtcaattct tgacgaagct ccgccatgac gaccttattc ttaccaccaa
     3421 cattgatacc tctcaccagc aaagcatagc gcgtcatctt ataccaattt atctaagaaa
     3481 ctttccccaa tcatagcagt ttccacacca aagttatcgg caacagtcgc tgaaatatct
     3541 gcaaaatgtc ctactggaat gagaccattt cctttaaagg cagggctata ggccaacaat
     3601 ggaatatatt cccgagtgtg atccgttcct gcatacgttg ggtcatttcc atggtccgca
     3661 gtaatcaaga gaaggtcatt ctctctcata gctgcgataa tttcaggtaa gcgttcatca
     3721 aactcatgca agcaatcacg gtaaccgtga gcattacgac gatggccgta aagggcatca
     3781 aagtcaacta ggtttgtgaa tgagaatcct ttttcaaact cagcaagtcc catagtcttc
     3841 aatagtgtat caattccatg actatttgac ttgttgtgac ccatgtcatg gttgatacca
     3901 gcaccgttaa agatatcgtt gattttaccc acagcataag tatcgatacc agcctcattc
     3961 aatttatcca aaacagttgg ggaaaatgga gatacagcca agtcacgacg gtttgccgta
     4021 cgagtgaagt tacctggttc acctacataa gggcgagcaa tgatgcgacc aagaagggca
     4081 ggacgctcaa gggtaatcga acgagcgtat tcacagatac ggtacaattc atccaaagga
     4141 ataatgtctt cgtgggcagc aatctgcaaa acagggtcag ctgaagtata gataatcaac
     4201 tctccagttt ccatctgacg tggtccaaaa tcatcgataa cagccgttcc tgaataaggt
     4261 ttgttggctt cacgaataac cttgcgtcct gagaattctt cgatttttgt caggatttct
     4321 tctgggaatc cgttccagaa agtatcgaaa ggctcagtaa tgttgagtcc catgatttcc
     4381 cagtgtccag tcatagtatc cttaccaaga gatacttcct ctaattttgt tgcatatcca
     4441 gttggattgc tttcagctgc tacagtctta agaggagttt cacgaggaat atttccaaga
     4501 cctattttag ccatgtttgg gacattcaaa ccaactgttt ttgaaatgtg tcccagtgtg
     4561 tcagaagctc catctggaac ccctgcattg acaaagttat tagcatctgg tgctgcaccg
     4621 attcctacag aatccagtac caccaaatga atacgattaa atttagacat cttatgcccc
     4681 cttctatttt cctttttttg aagttgaaat ctgaactcca tcccgtccag caacgatgac
     4741 cttatccacc atttggttga ataaaccatg ctccacaaca ccaacgacat ggtccaattc
     4801 ttgtccaaaa gcaattggat tttcaatgac atccaaggcg aggtcaatga taaaattctg
     4861 catatccgtc acaaaacgtt ggccgtcttt ttcacggaaa cttggtttgt agccagctcg
     4921 ttcaaaacga cgaaagacct gttctgcacc atactgaacc acttctactg gcaatttaaa
     4981 agcacctagt ttttcgacca gcttgctttc atccaccacc caaatgtatt cttttgatgg
     5041 tgtcgcaaca accttctcca tcagaagagc accaccacca cctttgattc cattaaactg
     5101 actatccact tcatctgcgc catcaactgt cacatcgaca aagtctactt ggtcaataga
     5161 tttgagtggg atattaaggc cttctgcctg tttagtggtc acactagaag tcgttacagc
     5221 tataatctgc aatccttctt ccttgatacg acgaccgatt tcttcgacaa aataataggc
     5281 agtcgagcct gttccaagtc caacgaccat tccatccttg acaaactcag cagccttgat
     5341 acctgccatt ttcttcagat tttccactca aacacctcca ttaaagagct acctctatta
     5401 taccatgtaa aggcttttat ttcatctaaa agtaccttta gaatactgct tatttatcct
     5461 tgaaattaaa tttttttaat aaaagcagaa ctctgacaaa tcactttaag tacggatgta
     5521 aaccaaatca actctaacaa aaatagattt acaagagtaa aaaaacgaac aaatttagtt
     5581 tcttagcgag aagaatacta aacgttcgtt ttttgtgaat taagaataat gatacaaatt
     5641 atcaatattt tttgtctaac tattttagac atatcttttc attactttag gaagacaatt
     5701 actaatctcc gttggcaaaa ccacatactg ttcttgagct agttcttggg ctatggctga
     5761 atgaagatgg gtcgctactg ccacacgttc gtaaagacta gcttgcttaa actggcctgc
     5821 aaatcctgca atcatcccag caagcgtatc tcccattccc ccagtcgcct gatagggacc
     5881 gccaacctgt aactggtaat aatctgattg gccagcttgc caaatacgag tagctgaacc
     5941 tttctccacc aaaattgttc cttgagggaa ggaagttagg gcactagccg ttgcatcttc
     6001 cttttgcttt tcaatcgtaa ttccagataa tttttcccat tctttttggt gaggagttag
     6061 gataagctgg cttgaggaaa atgacaaact tgtcctagca agaatggtca aggcacctcc
     6121 gtctacaatc aaaatctgat tttgtcttaa gccagcaaag acctgtttaa ctagatcttc
     6181 tccaaaagca tcgtctcgta aacccggccc cagcaagaca acttctgcct tctccaattg
     6241 ctcttttaac aactgctggt cttgaagaga aaaggccata gcctcaggta aatggctgtg
     6301 cagggctgga atattttccc tatctgttcc aacggtcacc aatcctgcac cgctttttac
     6361 agctgccaaa gcagccatga tgatggcacc accataagga taagttccac caagaaacag
     6421 cagacggcca tagtctcctt tatgactagt acgagaacgt tcaataataa ctttttctag
     6481 taaggcttga tcaatcactt tcatcgttct tcctcaacta tcctttatct gattgtttta
     6541 aaacaacttg atagactttc ttagtccact cttctaagtc ttctccagca tagtcaagat
     6601 aatgcaccct atcttgtatt ttagttggaa taatattttc aaattggctc ttattatgag
     6661 ttggaataat acaaatatac aactcactaa ataaattcaa catgtcctta atttttgcaa
     6721 catggtagcc atcaaaaata taaccagtaa ctttaatcaa atctgtatag ataaaatgaa
     6781 aactcactcc aggaataaac ttgtcttgta attctttctc tgaaggagct cgacctaata
     6841 aacgctccat cgctaatcga taaccagaac ttgtatttga ccaccctaac atgacataat
     6901 caaaataatc tatcggatcc gatgcagcat ttctactatc caaaaccaac tcttcagcat
     6961 ctcgcctaaa ggctttcaca gcagataaga gctgacctgt tttaaagata gaaatcgctg
     7021 catctatagt cgtagtatga ctacagtagt ctgaagtcac taacctctta tgtagatcat
     7081 aagtcgaggc tactctttta tctgtagggg taaatggtgg cagaaaggga tgctgacaga
     7141 tgtaatctaa aatttctttt tttaaacaag aatcagcaca ctcaatactt gtttgacgtt
     7201 tatgaagttt ttcgtaagct ataaatttag caattttttc taattcccac tcttgaatat
     7261 ttggataatc aattagtgct aggtagtata ttccatccca ttgtaaacta tattccctat
     7321 tataaatttt taaaatcatc taatcacctc actccattat acaacaaaaa ggaggcgcag
     7381 acctcctttt gtaatcttat atctaaaatt taatattcat ttctgccatt ttaaatatag
     7441 ctatagaaaa tacactctct tcagcgattt ttctcttatt ttctatccaa tgtccgaagt
     7501 gctgcttgat aagtttgctc catcagcata gtaatggtca tagggccgac acctccaggg
     7561 actggcgtaa tatagctagc aagtggggca actgcctcat aatccacatc cccacagagc
     7621 ttaccatttt catcacgatt catcccaacg tcaatgacta ccgcacctgg tttgacaaag
     7681 tcagcagtca caaacttggc acgaccgatt gcgaccacca gaatatctgc tttagcagcc
     7741 accttagcaa gattatgggt gcgtgagtgg gtcaaagtca ctgttgcatt cttggccaaa
     7801 agaagctgag ccatgggttt tccaacgata ttagaacgac cgatgacaac cgcattttta
     7861 ccttctaatt caatcccata ttcatgaaac atttccataa ttcctgcagg tgttgatgga
     7921 atcatgactg gatgtccaga ccaaaggcgt cccatattta gaggatggaa accatccaca
     7981 tccttttctg ggtcaatagc caatagaacc gcctcttcat caatgtgttt tggtaatggc
     8041 aactgaacca aaatcccatg ccaagctgga tcctgattgt atttagcaat caggtctaac
     8101 aattcctctt gagtaatggt ctctggaact cgcactactt cgctacggga accagccgca
     8161 agagctgacc tctccttgtt gcgaacgtag acttggctgg ctggattatc cccaaccaaa
     8221 atcactacca aaccaggcac tagagctgtt tcttccttta attttgcagt cttttcagcc
     8281 aactgcccct gcaatttggc cgctaaagct ttcccatcaa taatctgtgt catatcactt
     8341 ctcttttctt tcaaatatct tccattatac caaaaactgc tagtgccctc taacacttct
     8401 ttcctaggtt atcctatagt ttcaaactat aagaaaaagc tctggttgaa cttttcacta
     8461 atcttgtctt gaaattttaa aatagattat aaaaccttac tcaagaaatc tttagtccgt
     8521 tgttcttggg tttgttcaaa aatctgctca ggtgtcccat cttcaacaac cacaccgtct
     8581 gccataaaga taacacgatc tgccacctca cgggcaaatc ccatctcatg tgttacgata
     8641 accatagtca tccctgactt ggcaaggtct tgcataacag ccaatacctc acctaccatt
     8701 tcaggatcca aggctgaagt tggttcgtca aagagcaaaa catctggttc catagccaac
     8761 ccacgcgcga tggcaatccg ttgttgctgc ccacccgaca gactctgtgg ataagcgtct
     8821 gccttatctg gcaaaccaac tttttccaaa agctcttggg ctctcttctc agcaacttcc
     8881 ctgctttcac ctttagtctt gataggtgac aaggtaatat tttccatcac agtcatatta
     8941 ggaaagaggt taaattgttg gaaaaccatg cccatcttct cacgcatggc aaacaggtca
     9001 ttcttcttgt ccgtaatatc gactccctca aagataagct tccccttggt tgcttcctcc
     9061 aacaaattca tagagcgaag caaggtagat ttcccactcc ctgaaggacc gatgataacg
     9121 acaacttctc ctcttttaat ctcgaggttg atgcccttca atacttcatt ctttccaaaa
     9181 gatttatgta aattttcaat ttttatcaag gtttctgtca ttatttctta tctccttgtc
     9241 ccatatgttt ttcaaaagct ttcaaggcta ctgtcagaat agaggtcata atcaagtagt
     9301 aaaatgctgc aaataaaagt ggtgttaaag gtagataggt tgttgtagaa actgttgtag
     9361 ccccattcca caactccatg accccaatag ctgataagag ggagctgtcc ttgataatgg
     9421 tgataaattc gttccccaat gctggcaaga tatttttgac tgcttgtggc aaaatcacat
     9481 aacgcatcgc atttttaaga cgaatcccta gcgaataagc cgcctctagc tgaccttttg
     9541 gaaccgcatt gattccggca cgaacagtct ccgaaacata agcaccacta ttcatagaga
     9601 taatcaaaat ccctggaatc agacgcgaaa aatcaacacc taaaattcca atctgaatag
     9661 tcggagcatt gatatgcata agagcaaagg caatcataat ttgaaccatc atcggtgtcc
     9721 cacggaaaat ccaaacgtac aagttggcca accaaacaag cggtttaaac tttgaacgtt
     9781 gcccaaaagc caagacaaca cccaaaatag ttcccaaaaa gataacacag atagaaataa
     9841 gaatcgtcac aacagcccca tagttaaaat aaggtaaata cttaggtaaa aaagaaaaat
     9901 tcatagtcac actgctttct aaaatagaat actgtatgat tataacatgt tttgaattaa
     9961 aatttaaatt ttttatccaa tttatttaaa aatctttaaa ctagcagaat tagcgagaaa
    10021 tcttagtttt ttctagtcaa gttgccctcc tttatggtaa aatagtaacg ataagaaatg
    10081 aaggagaatc aaaatggaat cacatttggt tagaatcatc aaccgccttg aagcaatggc
    10141 aaacgatggc ggaaatttaa aacgtaattt tgaacgcgaa ggagttgttg ttgcagaagt
    10201 tgcgtacagc cacgacgaag aaaacggccc aatctttacc cttcgtgacg tagaagatcg
    10261 cgaaacgtat acgtttgata gcattgactt aatcgcaatg gaaatttacg aacttctcta
    10321 ctaatctaaa acaagttggg attgtccctg catgtctaag taaaatcctt tttgtctcac
    10381 aaataggatt tttttatagt ccaaattcac aaaaattttc attgtaacaa cttctcaaag
    10441 cttcaatctt tttacttgct ttacacccca atcctgttat aatgagagta tagaatttga
    10501 ctatttttga cctttaaaca agtcaggcta aagaaagaaa tgaggtagat gtatgctttg
    10561 tcaaaactgt aaaattaacg actcaacaat tcatctttac accaatctca atggaaaaca
    10621 aaaacaaatt gacctctgtc aaaactgcta taagattatc aaaacagatc ctaacaatag
    10681 cctcttcaaa ggtatgacgg atctgaacaa tcgtgacttc gatccctttg gtgatttctt
    10741 caatgatcta aacaatttca gaccttctag caatactcct cctattcccc caacccaatc
    10801 aggtggaggt tacggtggaa acggcggtta tggttcccaa aatcgtggat ctgctcaaac
    10861 tccgccacct agccaagaaa aaggcctgct ggaagaattt ggtattaatg taactgaaat
    10921 tgcccgtcgt ggagacattg accccgttat tgggcgcgac gatgagatta tccgtgtcat
    10981 cgagattctc aatcgtagaa ccaagaataa tcctgtcctt atcggtgaac ctggtgtcgg
    11041 aaaaacggcc gttgtcgaag gtctagctca gaaaattgtc gatggcgatg tgccacataa
    11101 actccaaggt aaacaagtca tccgtctgga tgtggttagc ttagttcaag gaacggggat
    11161 tcgaggacaa tttgaagaac gcatgcaaaa actcatggaa gaaattcgca aacgtgaaga
    11221 catcatcctc tttatcgatg aaatccatga aattgttggt gctggttctg cgagtgatgg
    11281 taatatggac gcaggaaata tcctcaagcc agcccttgct cgtggagaac tgcaactagt
    11341 cggtgctact accctcaatg aataccgtat cattgaaaag gatgctgccc tcgagcgtcg
    11401 tatgcagcct gttaaagtcg atgaaccaac ggtggatgaa acaatcacta ttctcaaagg
    11461 gattcaaaag aaatacgaag attaccacca cgttcaatat accgatgctg cgattgaagc
    11521 agctgcaact ctttccaatc gctacatcca agatcgcttc ttgcctgaca aggccattga
    11581 cctcctagat gaagctggtt ctaagatgaa cttgaccttg aattttgtgg atcctaaagt
    11641 aattgatcag cgcttgattg aggctgaaaa tctcaagtct caagctacac gagaagaaga
    11701 ttttgagaag gcggcctact tccgcgacca gattgccaag tataaggaaa tgcaaaagaa
    11761 aaagatcaca gaccaggata ctcctatcat cagcgagaaa actattgagc acattatcga
    11821 gcagaaaacc aatatccctg ttggtgattt gaaagagaaa gaacaatctc aactcatcca
    11881 tctagccgaa gatctcaagt ctcatgttat tggccaagat gatgcagtcg ataagattgc
    11941 caaggctatt cgccgtaatc gtgtcggact tggtacccct aaccgcccaa tcggaagctt
    12001 cctcttcgtt ggaccaactg gtgtcggtaa gacagaactt tccaaacaac tggctatcga
    12061 actttttggt tctgctgata gtatgattcg ctttgatatg agtgaataca tggaaaaaca
    12121 tagtgtggct aagttggtcg gcgctcctcc aggttatgtt ggctatgatg aggctggtca
    12181 attaactgaa aaagttcgcc acaatccgta ttctctcatc cttctcgatg aagtggaaaa
    12241 agctcaccca gatgttatgc acatgtttct tcaagtcttg gacgatggtc gtttgacaga
    12301 cgggcaagga cgcaccgtta gcttcaagga tgccatcatt atcatgacct caaatgcagg
    12361 tacaggaaag accgaagcta gcgttggatt tggtgctgct agagaaggac gtaccaattc
    12421 tgtcctcggt gaactcggta acttctttag cccagagttt atgaaccgtt ttgatggcat
    12481 tatcgaattt aaggctctca gcaaggataa cctccttcag attgtcgagc tcatgctagc
    12541 agatgttaac aagcgcctct ctagcaacaa cattcgtttg gatgtaactg acaaggtcaa
    12601 ggaaaagttg gttgacctag gttatgatcc aaaaatggga gcacgcccac ttcgtcggac
    12661 tattcaagac tatattgagg acacaatcac tgactactac cttgaaaatc caagcgaaaa
    12721 agatctcaaa gcagttatga ctagcaaggg aaacattcag attaaatctg ccaaaaaagc
    12781 tgaagttaaa agttctgaaa aagaaaaata aatcctatag aaaaggagta gaaaatgaaa
    12841 tttttctgct tcttttttta ctaaaataac tgtaatttct tgacagcttg ccctttgtcc
    12901 attatgat
//
LOCUS       DBERYS010000049        12701 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.049, whole genome shotgun sequence.
ACCESSION   DBERYS010000049 DBERYS010000000
VERSION     DBERYS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.049"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(329..2056)
                     /gene="ezrA"
                     /locus_tag="ACL8LA_001714"
     CDS             complement(329..2056)
                     /gene="ezrA"
                     /locus_tag="ACL8LA_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGS1813434.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2138..4084)
                     /gene="gyrB"
                     /locus_tag="ACL8LA_001715"
     CDS             complement(2138..4084)
                     /gene="gyrB"
                     /locus_tag="ACL8LA_001715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGS1813435.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4099..4662)
                     /locus_tag="ACL8LA_001716"
     CDS             complement(4099..4662)
                     /locus_tag="ACL8LA_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1813436.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4747..5301)
                     /locus_tag="ACL8LA_001717"
     CDS             complement(4747..5301)
                     /locus_tag="ACL8LA_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGS1813437.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5312..6535)
                     /locus_tag="ACL8LA_001718"
     CDS             complement(5312..6535)
                     /locus_tag="ACL8LA_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS1813438.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6572..9022)
                     /locus_tag="ACL8LA_001719"
     CDS             complement(6572..9022)
                     /locus_tag="ACL8LA_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGS1813439.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9222..10151)
                     /locus_tag="ACL8LA_001720"
     CDS             complement(9222..10151)
                     /locus_tag="ACL8LA_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGS1813440.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10291..10578
                     /locus_tag="ACL8LA_001721"
     CDS             10291..10578
                     /locus_tag="ACL8LA_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGS1813441.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10612..11946)
                     /gene="ciaH"
                     /locus_tag="ACL8LA_001722"
     CDS             complement(10612..11946)
                     /gene="ciaH"
                     /locus_tag="ACL8LA_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGS1813442.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11936..12610)
                     /gene="ciaR"
                     /locus_tag="ACL8LA_001723"
     CDS             complement(11936..12610)
                     /gene="ciaR"
                     /locus_tag="ACL8LA_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGS1813443.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3853 a         2718 c         2259 g         3871 t
ORIGIN      
        1 gatttttcaa tagacttcgt tattggacgg ttaccaagtt actcaaagta cagatttgaa
       61 gctgcagata agactgacga aatcacttta atactaaatg aaaatcaaaa agcaaactaa
      121 gaagctagcc tcaggctgta cttgagtacg gtaaggcgac gctgacgtga tttgaagaga
      181 ttttcgaaga gtataaaaca ctgttttgag gtggtggata aatcggctgg gtaaaaactc
      241 aatctcagga gaaaatgaag taaatctccc cacaactata gaaaaagatt tgattctgct
      301 cctcacacaa taaaatcttt ttcttttatt aaaaacgaat cgtttcacgt gttttctcat
      361 atgaggtaac aaagcgattg gttacaccag gctctgccac ttccaatgct tgagaaatct
      421 tgtcaaatga agcgtgataa tcaaattctt tttcaaaaat atctaaagct tcgttaaatg
      481 cttcttgaat gcgttcatca aatgagcgat agcggttaga atattgcaag agttgctctg
      541 tcaaagttgc atattgtaca atattataag tttccgtttc taaagcttcc atatcattcg
      601 ttgcaatttc aagaactcgg gtaacagatt caatgttaat cattttttgt tctaactcaa
      661 ccattaaatc ctcggtatta ttgcttgccg taaagaataa cttcaagaaa gtttgtggaa
      721 tacctggcag attgcgtttt tccatgtatc gcttgatagt atggagacga ttgacataaa
      781 cattggcctt ttgacgtgca ttaatatcat ctttctcaat ttgtgtcagg cgctcactaa
      841 ctgaaatttg ctcatcttca atatctttta gttgagtttg taaatcctca agattttctt
      901 caagaactga ataagcttgg gttggttctt cttgatttga agttacctca acaatagctg
      961 cctcaaaact ctctaattct gtctgaatac gacgaacatg gcttgcagct gtctcaggaa
     1021 gtaaataggt cttgttcaaa cgtgcaatat cttctcccaa taaagtatta ttctctttca
     1081 tatgttgaag gtaagttgga agagttgcaa gtagattttc cactactttc tgagcagcaa
     1141 tttctcgagt aaaaatatca tacaaggcat tgatttcctc ttgtgcctgt ccattctcat
     1201 attcggcatt atccaattcc aactgacgaa tattctcttg gtttttcttg aatgcttcat
     1261 aaagcaagtg gaaacgcgct tcaatatccg tttcaacaaa atgataatta gcatcaatta
     1321 gtttacgata accggattcc aaatcctgta attgatctgg caattctgta gaaagcgtcg
     1381 taaccaaggc tggaacacga tccacaatat gacttaaggc caaaatgtga ttttctgtat
     1441 tatccaaaat cactgcggct tccacagggt cacccgatga attcaaggtt acaaattgtg
     1501 aaaattcaga ttggatattt tctaattgtt tttcaatttc atccaaggct tgaccatact
     1561 gttctgaatt ttcagcaact ctatgctgaa gttcctcaaa taaatccaaa gcatgaagaa
     1621 cacgaccact atttttagat tcttgcttct ctaagtctgc caaagcattg cgaattgccg
     1681 caatatcttc gtcaatcaaa gtaatttgac tctcaatttg gtcaatttga tgactggcct
     1741 tgagaaaacg aaatgaatgg ttatagcctt ctgcttcaaa gagattattt tcaatatcgg
     1801 caaaagagtt gagagataaa tcgacccatt tttgattcca ttcacggaaa gccacttgac
     1861 tttgtccaat caagtgcata ttttttacag cttctacttc atcatttact ggaagattgt
     1921 atagttcttc ttttctttct tctagcgcct ctaatctccc ctcgtttcgc ttacgtagaa
     1981 agattgccac tacataagcc agaactaaaa tgactgcaat tgcaaccatt aaataaatta
     2041 gttgtccatt agacatatca aactcctttt ttacttgaaa caatcgtaac gattatatca
     2101 tattttttag accaagggaa ctacttctcc ctatttttta gacatcaagt gtactataga
     2161 cagcattttc ttcgataaac tcacgacgag gctctactcg atcccccatc aacatatcaa
     2221 agattttatc tgcttctgca gcatcatcta cagaaactct agccatcaag cgatgttcgg
     2281 gatccatggt tgtttcccac agctgatgat cgtccatttc acctagcccc ttataacgct
     2341 gaatagtcgg tttggtacga ccttcactat aacgggctaa agcttcttgg agtttgattt
     2401 cttgatctgc acccggctgg atatattctt taatctcgct tccaaccttg acaccataga
     2461 ttggtggttg ggcaatataa acataaccag cttctaggat tggtttcata taacgataaa
     2521 tcaaggttaa aagaagggta cgaatgtggg ctccatcgac atcggcatcg gtcatcaaaa
     2581 cgagtttttg gtaacgggct ttcgaaacat caaattctgc gccaaatcct gttcccatgg
     2641 ctgtgaaaag actacgaatt tcttcgttag ctagaatctt atccatactt gctttttcaa
     2701 cgttcaaaat cttaccgcga attggaagga tagcctgaaa ctcacggtta cgaccagatt
     2761 tggctgatcc accagctgag tctccttcga cgatgaagag ttctgtttca gcagggttat
     2821 tagaagaaca gtctgctagt ttccctggaa ggttggaaat ttccaaacca gattttttac
     2881 gtgtgacttc acgcgcacgc ttggcagcca cacgagcctt ggcagctaaa atcccttttt
     2941 ccacgatacg tttggcaatc tgtggatttt ccatgaggaa atcggagaag gcttcactga
     3001 agaggcgatt ggtaatcttg accacttcgc tatttcccaa tttggtcttg gtttgtcctt
     3061 caaactgtgg atttgggtgt ttaactgaga taactgcagt taatccttcg cgaacatctt
     3121 cccctgttaa attatcttca ttgtctttca gtaacttatt cttacgagca taatcattga
     3181 taacacgagt cagtgccgta cggaaacctt gttcatgcgt tccaccttca tgggtatgaa
     3241 tattattggc gaaactcatg acattttcat ggtaacctgt tgtgtactgc atggctacct
     3301 caactgtgat atcatccatc tcaccgtctg tatagattgg tgtatcaaag attacatcct
     3361 tgttctcgtt gatatattca acgtaactag caatcccacc ttcataatga taatgcttgg
     3421 tttgttccaa accttggcgc ttatctgtaa ttgaaatttg aagaccgcga tttagaaagg
     3481 ccaactcttg aatccgttta tttaatttat caaaatcaaa gattgttgtt tcagtgaaaa
     3541 tttctgggtc cggtgtgaag tgaaccgttg ttccagttct atctgtatcc ccaaccacct
     3601 caaggtctgc aacaacatga ccacgacggt attcttggta atgaatctta ccgtttttat
     3661 ggacatgaac gtctaattga gtggaaaggg cattaactac tgacgacccc accccgtgaa
     3721 gaccacctga aaccttgtat ccaccaccgc cgaactttcc tccagcatga aggactgtaa
     3781 agacggtctc aacagcagga cggcctgttt tttcctgaat atcgactggg ataccacgcc
     3841 catcatccac aacagtaatc gaatcatctg gctcaataaa aacttgaata tggctggcaa
     3901 atcctgccaa ggcctcgtca attgagttat caacaatttc ccagactaga tggtgaagac
     3961 cttcttttga ggttgatcca atgtacatcc ctggacgcat acgaacagcc tctaagccct
     4021 ctaaaacttg aatttgactg gcatcataat cctgtgcctg cagatttttg atttcttctg
     4081 tcatcttatt cctttttctt atatgtctaa tacttgtctt aaattcacga tactggtaaa
     4141 caagtgggta tcaagtcctg cagcttgacc tgcttcgata tcaatcggcc gatcaccaat
     4201 gacaagacca gagctaatct gatacttttc tcttaaataa agcatggatt cgggatttgg
     4261 ctttctctta aagcctgagc tagaagtcac cacttctgta aaataagctg ctatagaggt
     4321 tttttctaaa atttccaaaa cctgatcatt tcgatgagag accaaaaaat gacggccacc
     4381 ttgatttaaa atgtcttcca ataggtcaga aactccttca aataaaatcg ggtgttcaag
     4441 ctctctggct tcattttcct tgtacttttc taaaaaattc tctaaattgg gagcgaatgt
     4501 ctcaatcgca aaaggagtag aaacctttaa agcttgatag acactgtcat ggtcttgtgt
     4561 gataccatac agtgccaatg tttcaacaaa tgcagctgtt gaagtttcat aattatccag
     4621 taaagttcca cctaaatccc agatgtaatc gtgatatttc atacccttca ttatagcatt
     4681 tttttatgaa aaaagcgatg atttccctga gttttccaca taaaaaacga gagaataact
     4741 cccgttttac tgatttttac caaagacatc tcgatagagc attcctgttc gtaaactctc
     4801 tgcgtcccct cctagttgct ccaccaactc gtaggcaaag gcaagggctg ttgaaggacc
     4861 ccgactggtt gtcaactgac catctaccac tactgtttcc ttgacgtagt gaccatcaag
     4921 gatttgctct tgaacgccgt cataacaagt gtatcgctta tttttcaata tctctgcttg
     4981 attgagggca attggtgccg cacaaatggc tgctagtttc ttcccttctt gctcgaagct
     5041 ttgcaattct tgaatcaagg tctgattatc acgtaaatgt gcagaaccag gcatacctcc
     5101 aggaagaaca atcatatcat agtctgataa atctccatca aagacatgat ctgctcttac
     5161 ttggattgca tgcgaacccg ttacttgctc ttcaaaacca accatatcac atgtgatatt
     5221 ggctcgacgc aagacatcta caactgtcaa ggcttcaatt tcttcaaagc cctgagctaa
     5281 cataactgct acttttacca tgattttctc cttatttaat ttgttttaat acaacctttt
     5341 tccgtttgaa tgggactttt ccgctctttt cttcagctaa attagtctgg taactcatcg
     5401 ataaaagcaa accaacacca atcaaattac tgataatagc tgatccccct tgcgaaatga
     5461 aaggcaaggg aatccccgtc aaaggaagta gtccagtcac agcaccgata ttctcaaaga
     5521 tgtggaagag caacatcata atcaaacctg tggaaatata agtgtagaac tggttatttg
     5581 atttaagagt aatcttcaac atacggtaaa tcaacatgag atagagggca ataaccagga
     5641 cagagccaat aaagccaaaa tcttctgcaa taaccgtaaa aatcatatct gactctcgaa
     5701 ctgggataag cagattcgaa gcattaaatc cctgaccaaa taagccacca ctcccaatgg
     5761 caatctgccc ttgagcctgc tggtaagtcg ttgtttgggc aaactcaaag ggattgagcc
     5821 aagccaaaat ccgattaatt tggtaggtcg gcattccaat ctggtgaaga aaagctcgtc
     5881 cgtccttgct aataaagata gctaagaaac cagcaactcc tgttacagca gtcacaaata
     5941 ctgggataat aattttccaa gaaacccctg ataataaaac gattcctgag aaaatggcta
     6001 caaaaaccaa agccgtcccc aagtcacttt gaagtgctaa aagaactagg actggaatgg
     6061 taaagagaat catccagaaa attaacaaaa agtccagcgg aaccgtgcgt ctccattcct
     6121 tatgtttctt tgtaaattgg acaatgacac gagccaacat gaggatatag gatatcttca
     6181 taaattctga cggttgaaat agggtaattc catttattga tacccagttt ttggcacccg
     6241 ttgatgcaac taagcttgga ttataaaata caatcggcaa gatcataagt cccaagccta
     6301 aaatatatag aaagggggtc accttccaaa gaaattctgt attaaagagc atgaccacaa
     6361 aaccaatcac aagccccaag gcaatccagg cgacctgctg ccctaaaatg ggcagaatat
     6421 tattgggata atcatgacta acggctatat agatagccac cacaccgatg accagtagaa
     6481 aaaatactgg caagagcaaa ctataatcga ctctagagtc gagagaacgt ttcatatgta
     6541 ctaaccttat actttcatac aatactattt atcaaagttc attaaaaaat ctatcaatag
     6601 cctcgtcaac ttcggatcga gagatggttt taacagtcgc ttcttctgct agagatgcta
     6661 ctatttgttt gccgtatcgt tttccgacga ttctcctatc caaaataaga gttaaggaac
     6721 gttggtattc acgtctcata cttcttccca aagcctgttt taaacgaata atggccattg
     6781 gcaattgata atcataaaag gcatttttcc cttcttgatt cagttcttga ttaatctttt
     6841 tcgtcaaggg ttcttgagga ttttggaaag gaagcctcgg tacaacttga atcacagaag
     6901 gatggcttga aaaatcaact ccctcccaga aacttgctgc accaagcaag atttgttgtt
     6961 cacctttttc aaagcgtttc tttagctgat gaacatcccc atttttatac tgggccaagt
     7021 ggctaactgt aagtaaatcc gatactgcta gaagcatgtc tttagcggta aagagaacca
     7081 aaatcggttg ctggaaagct tgaatttcca ctagtaaagc agctacctct ctggcataga
     7141 cttctaagga ggtttctgtt accagaggga aatctttgac cataaccact tcttgttcct
     7201 gttttccccg agattcaatc ttgacaaatt tggcttcagg atagcctaaa aggtctgcca
     7261 aagaaaccct ctgactaatc tcaagagtag ccgatactcc caagacttgg caactctcag
     7321 gaagtaaaga ggacagaaga gtacggcctg attttgtaga agaaatctga attttctttt
     7381 gactcgtttc agttacttca agccagtaat caccttcagc tgtaaaatag cgaaccagct
     7441 catcaaagcc ttccacttct aattctgaaa aatactgatg gagattgtcc agagaatcta
     7501 agatattttt cctagattta ccagactgaa attgttctat caagtagaga cactcaaagc
     7561 gaatactttc tagtatccgt tgttgaaccc tgttttcttc tcctactaaa gccttatcaa
     7621 ttaaatcgat aatagattgt atatcgtagg tctcttgaag cagattttct agagctaaca
     7681 aaatcttttg gacttcatca ataatcagta aacggtcact gacaaattca ggattatctt
     7741 caagtctggt cacgagatag gcatgattag tcactaaaag cttgcaagtc tctgcccttt
     7801 cttgactacg tttccaaaaa tcttccgtca caaataagct ctgggatgat aaattcccat
     7861 catgacgaag gtctgctaga aaatgttggt aacggtagag ttgcccgatt tcatccaaat
     7921 ctcctgtctc tgtctcagta agccagacca agacttgcat tttaaagcgt ctaaataagc
     7981 gattttcatc attttcctgc aaggaatgat aaaaggcatc caacttcaga taattttgtg
     8041 gtccctttaa gctatgaata tctgtatgga acacttcctt gagacgttta ccttcttctt
     8101 ccatgatttg attttgaaga atctttgtcg gaacactaag aacaatttgt cgctctttgg
     8161 attgagataa agcgggtaag agatagccat aggttttccc aatccctgtc ggcgcttgaa
     8221 tcagagagaa aggttcatct ttcaatagca agccaacctc tttagcaaaa ctttcttgtt
     8281 cctccctcac ttcaaggttc aacagagaaa tatttttaga aaagtcttga gatagttttc
     8341 gtagctccag agaagctgcc gttttcttaa aatacagacc ttgaacttgg accaagtctg
     8401 gagaactcag gatagattgg ttgcgataag tttcctcaat aaccaggtaa gactcatata
     8461 ggagagcgtc agccatttcc agcaagcgtt ccaagagacc tttaggaagc tgggtcatct
     8521 tttcccgtaa aaaaagaagt aattctgctg tagcttgggc atctgaaagg gctgtgtgtg
     8581 cgtgtttaag aggaattcct aattctcgac acaaaatcgg caagctatat ttttccagtt
     8641 cagggaaaaa gacctgggcc aattcgaccg tatcaacacg agggtttctt agctcatagc
     8701 cttcaaaaaa taaattttcc gccaagagat tagcatcaaa ctgaacatta tgggctacaa
     8761 aaatcccatc ctccaccaag tcaaagattt ttctggcaac ttgcgaaaaa tcaggtgctt
     8821 gcgccagacg ttggtctgtc aatcctgtca gttctttgat atgagcatcc aagggttcat
     8881 gtggattgac atccgtcgta tagtgatcga cgatttctcc atcctcaatc acgacaattc
     8941 ccacttggat aattttagcc ttactacctg tgctagttgc ctctaaatcc accacaacat
     9001 agcgattacc aatcgttttc attataaaaa attataccaa aaaaagggcc atttggcacc
     9061 tgcaaacata ggataatttt caaactcaag aacactactt ttcttagaaa tcccatcgaa
     9121 tagagcattc tagcctgctg atttttttaa aatagtgaag aaatacgaaa aattgagaga
     9181 agaaatgttt tcatcttcct tctctcaacg aatcatgtat tttatttaac catgtcagga
     9241 tcgtagtcat aaaatcctgt atcaaggaaa cggtttttag ggtgaagttt acgaacttct
     9301 tcatccagca agaaggcttg gtcatggcta aagttgccct cttgaatcaa gctacgcgct
     9361 tggataaaga gttttgcaat ctcaacaaag ttctgaccag gagtgtagag cccttctagt
     9421 ttccagcgag taaagccatg ctccaccaat tctgttaatt tgatcatcaa atcaaggtca
     9481 ttgttggcaa agatatgggt cccatgatta tcttcaaaaa tggaatagtg gctctctgga
     9541 tcacttggct cagccaagaa gaggtcacgt ttatgcgtct tttcatcatc gatatgtgta
     9601 aagttatagt agttttgcaa gagtggacgt ttagaatgat ggatgacgct agcaccgtaa
     9661 accaaaactt cagcaggaat ttccaaaatc tctggcattt tgaaaagttc agctgatgga
     9721 atttcacgcg ccaaaacagc ctcagatgcg ccagcctttt gtccccagaa gttaatctga
     9781 cgactgctag ttaccatggt tgaagcatcg tagatggtct taaatgaata accatcgcgg
     9841 ttaactacgt aaaagacgcc tgcatcccca atcgtaatat agtctgtctt gatttcttcc
     9901 aagaagtcta agaaaggctt gatacggtcc atcatatctt ggtgcatgag agcattgacc
     9961 gcaacgatca attccttacc agcatcatga accaacttag cgatttcacg taattggtca
    10021 taactaaagg tcgttggcag acgaaggcca aaatctttct caccgacata gatacggtct
    10081 acgccagctt cgagtagttg ttcaacttgt tcaatacttt cagcagttgc tgtaatgaca
    10141 atcttttcca taagaaaaat tataccacat ttctccaaaa tcagccatgc tttgaaaatg
    10201 aaaagggact ttattctttt tgtaaccttt ctgtaataat tctctgccga aaaaatgata
    10261 aaataggtat gtactgttaa ggagagaatc atgcccgtaa gaaaattaca atcctatgag
    10321 gtagactatc aagaagaatt aaaccagcag cttcctcatt accaagctta tacacctgaa
    10381 gcgcaatctg atgccaatct caaggaaatc ctatttttta ttaatatcgc tgttttttgt
    10441 atctgtattg ctatctttag ttttatcttt ttagcattaa aattatcaac tgctcttgcc
    10501 tttgccgcag caatcggatt cagcttactt gttttaaaag ttcaacggtc tattatcaaa
    10561 cgaaaacgta gaagataaat ccaaaatatc gccccaattg gagcgatatt tttatttttt
    10621 ctttttagat ggtgtctgaa tggcaatctt cacttcaaag attgttccct tgggtttatt
    10681 atctttgaca gtaacagttc cttttagagc atctacaatt tgcttggcta gggataatcc
    10741 taaaccaaaa ccaccttttt gccgggttct agctttgtct actcgataaa aacggtcaaa
    10801 aattttcttt ttatcttctg tcgaaatacc gattccatta tcagaaacaa gtaaataaag
    10861 attgcgatcg gtcgccgaga taagaaaatc aatttcacca tcctcctcag tatacttgac
    10921 ggcattatcg aaaagaatgg tcatcagttg tttcagaaga agctgatctg tgacaattgt
    10981 tcgatggata cgattttcaa aacggaagac acgattattt tccgaagcaa tcatctcata
    11041 gtttgtgaaa gttgtattaa aaaagctagt tggaacttct gcaagctccg gcttaatccc
    11101 atcatctctc cgagctaagt tcagcaagct tgtcgttaaa aaacgcatat ttcggacttc
    11161 ttccaaactc gatgcaatgc tttcgctcac atccataatg gtagcttctg gcttacgaaa
    11221 aagggtctct aagcgatttt gcaaaactgc gagtggagtt cgtaactcat gactggcatt
    11281 ttccacaaaa gactgttgct tctgcatact ctcaagcagg ggcctaacac tgaccctagc
    11341 tagatagaga ctggcaagta aagacaaaat ccagaaacta gccatcacga ccacaatcaa
    11401 ttgctcatgc ttttgactag cctgctccaa ctgactggta ttaatcaaga cagcagcata
    11461 cttgatattg gttgaaaccg aactaatatt ggtctccatc aaaatcacac gatagatttc
    11521 ttcctgccca tagctattaa aaacctgaat ctggtagata tgtcctagtt ctttcttttc
    11581 taacttaatc ttatccaagc ccaaaaatcg atttccagaa agaagttgag taaagtcttt
    11641 atcaaagaga atgacttccg tattggaact gacattaggt tttatttcta ctttactagc
    11701 gtccgcccta gcattttcta aatctttaat ctcttctgtt gccctattta tagccagctg
    11761 aataactgct tgaggatttt cactcagtcc atgaagctta tcgtccaccg aagtataaag
    11821 actcgaatgc atgacttgta aaataatcag agtcattgta gaaaaaatca gggtgaagac
    11881 accgaagttg cggataaaat aactaaagtc atccgcatac catgtttttt taagtttact
    11941 gaacatcttt taaaagatac ccaacactac gcaaggtttg caaattctct gcaaaagtgg
    12001 ttccctttaa tttcttacgg acttttgaaa catagacttc gacaaccgaa atcgttgtat
    12061 cactatcaaa tccccataga cggtcaaaaa tctgcgtctt aggcaaaatc acattttgat
    12121 tttgaaggaa ataaactagt aaatcgaact ctttccccag caattcgaca ggagtatctt
    12181 caactttaac ggtattggtt gataaattaa ccacgatatt cccataagtc aaggtgtttt
    12241 cattaaactt ccctgaacgt ttgagaaggg cctgaattcg cattttaagt tcttctaggt
    12301 agaaaggttt ggtcagataa tcatccgctc ccagttcaaa tccatgtccc ttgtcatcca
    12361 aactttcctt ggcagtcata atcagaactg gtgtcgtaat tcccttttca cgcaattctt
    12421 ttaagacttg gaaaccattt ttttctggca acatcaaatc cagcaaaatc aagtcataga
    12481 caccactctc agcttcgtag agaccttctt ctccatcaaa tacctgcata acatccgcaa
    12541 aatcgtctaa aaagtcaaat actgaatttg acagacctag gtcatcctca accaataaga
    12601 tttttatcat gagaaactcc tccttattaa aactattata ccaaatttgc cttaaaaaaa
    12661 actcaactct ctgcatttta catgagatag ctgagttttc t
//
LOCUS       DBERYS010000050        12598 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.050, whole genome shotgun sequence.
ACCESSION   DBERYS010000050 DBERYS010000000
VERSION     DBERYS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.050"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(128..1225)
                     /locus_tag="ACL8LA_001724"
     CDS             complement(128..1225)
                     /locus_tag="ACL8LA_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS1813444.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILAQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNLSIAQQNFGRVPIRIETN"
     gene            complement(1235..1975)
                     /locus_tag="ACL8LA_001725"
     CDS             complement(1235..1975)
                     /locus_tag="ACL8LA_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1813445.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2295..2648)
                     /gene="rsfS"
                     /locus_tag="ACL8LA_001726"
     CDS             complement(2295..2648)
                     /gene="rsfS"
                     /locus_tag="ACL8LA_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGS1813446.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2661..3161)
                     /locus_tag="ACL8LA_001727"
     CDS             complement(2661..3161)
                     /locus_tag="ACL8LA_001727"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS1813447.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(3162..3755)
                     /gene="yqeK"
                     /locus_tag="ACL8LA_001728"
     CDS             complement(3162..3755)
                     /gene="yqeK"
                     /locus_tag="ACL8LA_001728"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGS1813448.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(3755..4384)
                     /locus_tag="ACL8LA_001729"
     CDS             complement(3755..4384)
                     /locus_tag="ACL8LA_001729"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGS1813449.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4436..4747)
                     /gene="yhbY"
                     /locus_tag="ACL8LA_001730"
     CDS             complement(4436..4747)
                     /gene="yhbY"
                     /locus_tag="ACL8LA_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGS1813450.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4961..6067)
                     /gene="yqeH"
                     /locus_tag="ACL8LA_001731"
     CDS             complement(4961..6067)
                     /gene="yqeH"
                     /locus_tag="ACL8LA_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGS1813451.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFITGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(6070..6597)
                     /locus_tag="ACL8LA_001732"
     CDS             complement(6070..6597)
                     /locus_tag="ACL8LA_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGS1813452.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            complement(6612..7520)
                     /locus_tag="ACL8LA_001733"
     CDS             complement(6612..7520)
                     /locus_tag="ACL8LA_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS1813453.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDKPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7577..8137)
                     /locus_tag="ACL8LA_001734"
     CDS             complement(7577..8137)
                     /locus_tag="ACL8LA_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGS1813454.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8276..9485
                     /gene="sstT"
                     /locus_tag="ACL8LA_001735"
                     /pseudo
     CDS             8276..9485
                     /gene="sstT"
                     /locus_tag="ACL8LA_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9553..10506
                     /locus_tag="ACL8LA_001736"
     CDS             9553..10506
                     /locus_tag="ACL8LA_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS1813455.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            10649..11029
                     /locus_tag="ACL8LA_001737"
     CDS             10649..11029
                     /locus_tag="ACL8LA_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS1813456.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETVAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            11128..11349
                     /locus_tag="ACL8LA_001738"
     CDS             11128..11349
                     /locus_tag="ACL8LA_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS1813457.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            11366..11680
                     /gene="trxA"
                     /locus_tag="ACL8LA_001739"
     CDS             11366..11680
                     /gene="trxA"
                     /locus_tag="ACL8LA_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS1813458.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            11985..12518
                     /gene="queF"
                     /locus_tag="ACL8LA_001740"
     CDS             11985..12518
                     /gene="queF"
                     /locus_tag="ACL8LA_001740"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGS1813459.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         3502 a         2866 c         2491 g         3739 t
ORIGIN      
        1 agagcaaact aggaaactag ccgcaggctg tacttgagta cggcaaggcg acgttgacgc
       61 gatttgaatt tgattttcga agagtattat tcgtaaaaaa tctcaaaaag tccacctttc
      121 ggtagactta gtttgtttct attctaatcg gcactcttcc aaaattctgt tgtgctatac
      181 ttagatttcc tagttggtaa atctggtctg ctttttgggt catagcatcc caaggttctt
      241 tgccaattcg gctgactaga ctgacctgac ctttcaaaga cttgagatgt tgcctgcctt
      301 tttcagtaaa tccaaggaca tgaatggctt ctggcaagac attttctcta gcctgcgcca
      361 agatatagga caagaggcgt ctgacacgcg ccttggtgta acgtttggta gtcactaact
      421 cgaccaattc ttccacagac tgggctgttt taatagcttc tttaatgcgc acagccattt
      481 cttgattgac ctgatagatg gtggttaggt cgggatttga caagatttga tagcggagca
      541 agggaaaata gtcttcccag atcaccttac tggcctgctc aaatagggca acagaaggca
      601 taaaacgttc taagaaatct tggtccttct gatgttgacg gagggctgtc gccgaggcaa
      661 agtccacatc tttattcaca gaatggtaac ctgccccctg acgctgaatc ggatgcagct
      721 tgatattccg tcctgcaacc gccttggcat aggccagagc aagaacatga ttgggggtat
      781 tacctgaaaa atcaagacca gcaaattcct tccacatagc ttgggttttc tggggatagg
      841 aaagggaatc aggcagattt tccacaaatt tctccatctc agtacctttc tctgtgtata
      901 agtcagcgat tttctgataa tctcgaactt cctctgtccc aaaaactaga ctatcaatgc
      961 ccaagcgatc caagatatcc acagctcctt gaccaaagaa atcagctgcc tgaacactga
     1021 ctaaaaaggg caattccact accaagtctg cgccattttc cagcgccatc tgggcccttg
     1081 tccacttatc cacaatagca ggctctccac gctgcatgaa attccctgac atggcaacaa
     1141 ttttcagtcc ctctgcctga tccagcaggt atttatggcc attatgaaaa ggattgaact
     1201 ctgcgataat acctgtgatg gtcatgatgt tctcctactt ctgcgccaca aaaaaccaac
     1261 gggtgctagt ttctgttggc tccttatcct caaagtctgc atagagtttg aaggacttaa
     1321 atccagcctg ttccagcaag atatcatagg tcaagatctc ataggtcctc tcctcatgta
     1381 cttcatcgtg gcgactaaag gaaccgtcag cctccttgat aaagaaagtc agctcatgca
     1441 cgatggagtg aggagcttcg tcctcatatg tatcccaaag catggcaaaa tcttccgcat
     1501 tttcatggta ggaatagcca gggaatactt catctgtctg gtaggtcgag tgcacgtcaa
     1561 agatgaaaac tccctcttca ttcagcgcat tgtacacgtc cttaaagacg tcccctactt
     1621 ccacctcatc ctgcatatag cagatagagt ccgaataaca cgtgacaaaa tcgtatttcc
     1681 ctgccttgga caaattcagc atattacctt ctataaaagc aatcttttgc ttggctgaag
     1741 ttgctctctt ctccgcaatc ttcaacatat ccgcactcaa gtcaagtcca gtcacatcaa
     1801 aaccagcctg agagaagcgc actgattgaa ttcctgtccc acaagccaat tccaagagtt
     1861 tctttctctc cttggtctta ggcaaatgac gcagagaaaa attcgtccat ttgtcgtata
     1921 aactatcgtc catgaccgca tcatagaccg ccgcaaaggt ttcataagtc gccataaaac
     1981 atgataccaa gtccgcccgt aattcgatag aaaattagga gccttacgat ggagccgagc
     2041 tccaaggaag gctatctatt ttccgagaat tacaggacgg gttcaaatcc tttctaagat
     2101 acaaagagcc ttaaaagcaa agagtacata ggaaactttc ttccctatcg tcagatacaa
     2161 agaaagttac tttttatctc agcttttagc tcgtgttcag tttcgaaatg aaacaaacac
     2221 gaggcccttt acctttctat caatttttct aaaataagca aagaaaccca gttgactaca
     2281 actgagttca tcttctaagc aagagcttct gaaatatcta ctgaattcgc ctcatgccat
     2341 agcttctcta gattataatg ggcacgcatt tcttctgaaa agatatgcac gacaacagca
     2401 ccgaggtcca gtaagaccca gcctccagct gcatcgcctt cgacatggct acctttaaag
     2461 cctgcttgag ctactttttc acggatatta gctgcgatag cgtccaactg acggctattc
     2521 attgagctag tgatgacaaa gtagtccgtc acactagtca aatcttgtac atcaagtgcg
     2581 aggatatcct ccgcacgttt ctcatcagcc gctttcacga ctagttctag taattctttt
     2641 tcgttcattt aatcctcttt ttagaaaata tgtttgtaat ctagaacatc tgcaaagcgc
     2701 tcctcccaaa tgtcctcata aaattcatta atcgtttctg ctggaatgtc atccccatca
     2761 aaggttgtga cagcaccttc tggaacaata agctgatagc catattcaaa ggcaaccttg
     2821 acagaggtat ccacacaata ttctgtctgc ataccacata aaactaattt ttcaatcccc
     2881 tgcttatcca agtattcttt taagccagtt tctttgaaaa tactgttata cttcttctga
     2941 aagacctttt cagcaggttt tcgatttaaa agcgcagata actgccaatc ttctgatgtt
     3001 tgagcttcag agttctcaat atgttgaaca tagataattt caatattctt gcttctagcc
     3061 tggttttgta aataagaaat tttttccaat agactttttg tctggaaccc agtttctact
     3121 aaaacattct gaacatcaat gattataaaa gccgtcttca tttagtcctc tttcaaatag
     3181 tgcacaaagg cgttataggt ttcaagggtt tggggataga tggggaatcc ctgatgagct
     3241 agatactcca cggtacgagc tgtttcgtag gccaccgcct tattgagcga tagacttgca
     3301 atctcacgcg ccacatccac tccaggaaat gcccgattgt gctcgatata gtctgcgacg
     3361 tagatgacct tatctaggtc tgtcatttga ccagctccga ctgtatgaat ttcaatggct
     3421 cgcagaattt cagaatcatg caaatccaaa tcttcctgaa ttttgtaaat tccgaccata
     3481 ccatgccaga cattgttgcc ccagtttttg aggtcagggt ctagctggta acggtcaatc
     3541 aaggctagaa attcctgatc tgacaacttt ttagcatagt catgaagaag acctgctaga
     3601 cttgctttct caacatctac accaaagcgc tgagctaatt ccatagcggc acgctccaca
     3661 cctagacaat gggttagacg tttctcaggt agaagctctg ccattttttc caacaaggcc
     3721 tcacgggagc agttgatata gtcttgatag gacatcagta gagcccctcc ttctcgatgt
     3781 agtctagcac tggctgaggt aggagaaagt tgggtttccg accttgggca aggaagtccc
     3841 gcaccatgct ggacgagata tccatgagcg gtacgtccac ccagataact ggataggaag
     3901 tccctacctt gtagcgtgga cgctgaaccc ccacaaactg aaccatgtca accagttcat
     3961 caattcggta ccacttaggc agatagtcaa ccatgtcggc accgatgata aagtaataat
     4021 ccgtatctgg attcttctct gtcaaaatct tcatggtgtc gtaggtgtag gaaataccct
     4081 tgcgctccaa ctcaatggtt tcaatgacta ggccgtcaat tccctcaatt gccaactcaa
     4141 gcatcttgag acgatggtgt tcagggatgg tttccttttt atcaacgtga ggaggttggt
     4201 attcaggcat gagcagaact tgatccagtc ccaactgttg ccgtacttga tccgcaacaa
     4261 taagatgggc attgtgaaca gggttaaaat tcccccctaa aatcccaact tgtttgcgtt
     4321 ttttctcctt gatttctggc tccaactcta ccttggtaaa gggagtcaat agttcgattg
     4381 tcataggcta gtccccttta tttctctatg aaaacagtta tttggagttt cggtcttaga
     4441 tttctttgac tttcttagaa atcttgcgat tttctttctt gctagattgt ttaaacaaaa
     4501 tcaagatgcg tcctattttt tggactgtat ccacaccgat ttcttcttcc aaaatttcag
     4561 ctacttcgtg gatgttttca tctgtgtttt gtaagagagt aaccttgatt aattcacgcg
     4621 catcaagagc ttgacggacg ctggttttga tttggtcgtt gagtccattt ttcccgattt
     4681 ggatgatagg tttgagggtg tgtgcctggc tgttgaggaa ggcacgttgt tttgatgtta
     4741 atgacataat tttcttcctt gttttttgat agttatttta ggtggtgagg gacggtcgga
     4801 actaattttc caaagatttc atctttgaaa aatggatttc ctgacctcac aattgagcct
     4861 tggtctcaat tgtgcccctt gccaatctac agattggcaa tcacaccacg gcaataacta
     4921 tcgctatatg agctcacttt gttcgctcag cgattttgtt ttaaataatt gcttttcgtg
     4981 tgacgacggc gacgccttct ggtgcccaga cggcgacttt tgctgtgcct gttacacgaa
     5041 tccagcctag gcctgagatg actaggtctg tcttatcttt aatggtaaag acatgctgga
     5101 ctagccttgg gaaatcttct ttttccttgc tatttggtgg tgtcagaaga gttcccaggt
     5161 gcttatcgta gaaagcacta gctccttcaa gcttgctacg atggagtttg agttcattgt
     5221 caaagaaagc agtaaatcct tgcttttctc ctgttataaa gtcaaagcgt cccaaaccac
     5281 ctaaaaatag ggtttgctca ggattaagct gataggtctt aggcttgatt tcctttttag
     5341 gactgacata cttgaggttt ttggccgtca aatagtgagc catctggtgg cggtggataa
     5401 ttcccggcgt atcgtaaata taagatccgt cgtcaagcgg aatctctatt ttgtccaagg
     5461 ttgtccctgg gaagcgtgaa gtagtgatga cattctgatc acccgtgatt tcttggataa
     5521 tagcattgat tagagttgat tttccaacgt tggtcacacc gaccacatag acatcgcggc
     5581 ccttacggta gtgttcaatc ttgtcaatga cttccttaat ggcatgttta ttttgtgctg
     5641 aagttaggac cacatcgact ggacgaagac cttcttcatg ggcacgtttc atgagccact
     5701 ggctaatctt accagactta actgacttag gaaggatatc ttttttattt cctaccaaga
     5761 ggacatcatt gcccgagacg aaacgtggta aacctgggat gacagatcca ttaaaatcaa
     5821 agatatcaat gacattgacc actaaagcat cactgtctcc cacctcgtgc aagagcttga
     5881 ggaaatcatc gttcgtcaac tggacatctg tgatttcatt gtagtggcgg agacggaaac
     5941 agcgttggca atagacttcg ccagtctcca aacctttttc aagtgccgac tggggggtaa
     6001 aaccaagacc agctttatct gtcgtctgaa tggttgctcc acaaccaata cagagaattt
     6061 cttccatagt taaattcctt ttttatatgt aatcggtccg tacttttcag tgatttttct
     6121 cataacacga cgctcacgag ttcggttaat ctgcgttttg attgagtcat gttggaccaa
     6181 gggtttgact aaaattgacc gaatccctgc acggtgggct gctcgtatat ctgtcatgag
     6241 ctggtcacca accatgacca cttccttttt gtcatagtgg aattccttca tggcacggtc
     6301 aataccaaat gtgaagggct tcaaggccca gtaaacgtaa tcaatcccaa atttctcaac
     6361 tgctcgttga acgcgttttt tggtgttatt tgacactacg ataatgccaa tacccgcgtc
     6421 ccgaaggtca tgtagccatt gcttcatctc tggcgttcca tcagggttgt tccaagcaat
     6481 gagggtatta tccaaatcga ccaaaacagc cttgattccc tgcgcctgca ggcttgggac
     6541 tgtcagatca tagactgctt ccacagcaaa atctggtata taattttcaa tcgccattct
     6601 ggctcctttt cttaactttt tttcgcaatt ttccttagta acaaggataa aacaatccac
     6661 aaacctgcac tagccaaact gatatagagc caagcatggg gcttatctgt taaaggtaag
     6721 ggaacattca ttccgaaaaa gcctgtcacg actgccaaaa cagctagcaa gactgaaatg
     6781 atagtcaagg ttgtcaaatt gtcattcaga ttattgttta gaatattgtt gtaagaggct
     6841 gaaagctgct gtaaaatctg agagattagg tctgtcatgg ataccagctg atgagcctca
     6901 atcatggcat catcaaactg ttctctctca atctcatcaa aactacgata caaggcatga
     6961 ccttgaatat gctctaacaa aatccgattt tgtttggcag ctgccgtcag ataaaccata
     7021 ccagtctcca aatcagacag ggcaaagagg tttttcttgg tagttcgctg gcgcaagagg
     7081 tcattgacct catccctact cttgtccatc tgctcaatga caggatagta ggcattgctg
     7141 atgatttcca gactggcaaa gagaaactta taaatcgaaa gcgtgtcatg gttctccaga
     7201 taacgagtca tctgttcaat gacataggcg ttcttggtat tactaatggt aatcaggcga
     7261 cgatgctcga caataaaggt catgggaaaa gtctcatagt aggccttgtc cttttttacg
     7321 tctaggacat tatagataaa ggtaaccgtc ccactttcac ggtggtagtc catgtgggcg
     7381 cgctcgtttc tatccagtgc gtattcaatg gtttccttgt ccaaaccgta aatttcagaa
     7441 aggtcttcta gtttattcaa ctttcctagg tctaggtcta tccaggtaca accattgccc
     7501 aactgctttt ctaaaaccat cttttctcct tattaaactc cctctattgt accacaaatt
     7561 acttaaaata acaggtctag tctgtgcgtg agaaattgct aacaactgtg atatttcggt
     7621 tagggacaaa gtgaagggct tgctcctcgc tacgaagctg tgtcgtacga attcccacac
     7681 tgacaacctt acccgataca gtaatcggtc catttgtcag aacgacctca tctcccacat
     7741 ccagttgacg ttcaaagagg atgaaaaagc cattgatgac atcagacaga aagccttggg
     7801 ctcccatacc aatcgctacc ccagcaatac cagctccagc cagcaaacta gaaactggca
     7861 aacctaaaat cgacaaaatg cagtagagta aaaagaaata tagcgtataa ttaaacacat
     7921 tttctagtaa acgtgagatg gttttttggc gtccgacatc atgacgagac atttttagag
     7981 aaggtttgac aattctctgc accatggtat gaagcatttt tttagcaata taaaatacaa
     8041 tcaaaagcag taaaagagaa atgaccttgg ttagaatatt ctcgataatg gttgtcacat
     8101 ctagcttttc aatataagcc tgaataaatt tttgcatata aaactccttt atttcattat
     8161 accatatttt catcagtctc attctccata aatattcaaa aaatagacag aaaattcttg
     8221 attttgtcta tcatttatac tataatcata atcactacac aaaaaggaga ttattatgaa
     8281 aagaatcatt catgcttgga ataaggcaag ccttgtcaag cgtatcttga ttggtatgct
     8341 ttgtggggaa tcctaggact gacccttcct aatctctcag ggattgggct actcggggat
     8401 ttattcgttg gaggtctgaa agctgttgct cctatcctag tctttgccct cgttgccaat
     8461 gccctttccc aacatcaaaa gggacaagat agcaatatga aaactgttgt tttcttgtat
     8521 atcctatgat agggactttc gccgctgctc ttgtagctgt actagcaagt ttcatcgtcc
     8581 ctattgaaat taccctaaat agtgccaata ctgaaattgc accaccagat gggattgggc
     8641 aggtcctcag caacctcttg ctcaaactgg ttgacagccc agtcaacgcc ctgcttactg
     8701 ctaactatat tggaatctta tcttgggcag tcatttttgg aatcgctatg agagaagcta
     8761 gtaaaaatag taaagaattg ctaaaaacta tcgctgacgt gacttctaaa attgtcgaat
     8821 ggatcatcaa tctggctcca tttggaatcc ttggtcttgt ttttaaagcc atttctgaca
     8881 agggagtcgg aagccttgcc aactacggta ttttattggt tccattagta acgactatgc
     8941 tttttgttgc ccctgtggtc aaccctttga ttgccttctt ctttatgaga cgcaatcctt
     9001 actctctagt ttggaactgc ctccgtgtca gcggtgtgac agcctttttc actcgtagtt
     9061 ctgcgactaa cattcctgtc aacatgaaac tctgccatga ccttggactc aacccagata
     9121 cctattctgt ttctatccca ctcggttcta ctatcaatat ggctggagta gcgattacca
     9181 ttaacctttt gacccttgct gcagttaaca ctcttggaat tcctgttgac tttgccacag
     9241 cctttgtcct cagtgtggta gcagctatct catcctgtga tgcttcaggt attgccggag
     9301 gttccctcct tcttatccca gttgcttgta gccttttcgg tatttctaac gatattgcca
     9361 tacaaattgt tggggttggt tttgtgattg gtgtcatcca agactcatgt gaaacagccc
     9421 ttaactcttc tacagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa
     9481 aataactcat caaggcaagc ctgctcatgt cttgtctttt acgcttttat tctaacttat
     9541 taggaaattc ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt
     9601 gcctgcctgc ttgcttattt tctcaatctt tcgtcagcag tttcggctgg aattatcgct
     9661 ctcttgagcc tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt
     9721 tctatgcttc tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc
     9781 tggagtctcg gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa
     9841 attggcatca caccaagcac tgttttggtt agccatctct tggttcaaga gtcaacctct
     9901 ccagaccttc tagtcaatga attccttctc tttgctattg gtacaggatt tgccttgctt
     9961 gttaatctct atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa
    10021 gaaaagttaa aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc
    10081 aaccgagcac agctggtagc agaattagac acgcttttga aagaagccct cagactggtc
    10141 tatttggatc actctgacca cctctttcac cagaccgact accatatcca ctactttgag
    10201 atgagacagc gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac
    10261 cttgccgcca gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg
    10321 agccagacca atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc
    10381 cggaaccgca gtctgcccaa gacaagagaa gaatttgaaa cccgcgccac ccttcttcaa
    10441 ctcctacgtg aagccaaaac cttcatccaa gtaaaagttg atttttacca aaaatataga
    10501 cagtaaaaaa taaaaccact aaaaaacaga gcttgccagc attcttgaaa agtagtataa
    10561 ttattgcatg cgcaatcatc ttgtgacgca gagactgccc gttaatggac tttcttctat
    10621 ttatgactct aaaaagaaag gagacactat gacctatttg gaaaaatggt ttgacttcaa
    10681 tcggcgtcag aaagaaattg aaagtctctt ggaagagacc gttgcccaac agagcgagca
    10741 aagtctgacc ttgaaagagt tctacctgct ctactatctg gacttggctg aagaaaaatc
    10801 tctacgccag attgacctgc cagataagct tcatctgagc ccgagcgcag tttctaggat
    10861 ggtggctcgc ttggaagcta aaaattgcgg tctactcagt cgcatgtgtt gccatcaaga
    10921 tagacgctct agttttatct gcctgacaaa tgatgggcaa aagatactgg cctcgctaca
    10981 aaagactgtc gaagaaagct tggaagcagg tttggatttc ttgatttaag atgattttag
    11041 aaaaagttgc gtgcgcaatc atttttcttg acattctctt ttataaggag taaaataaag
    11101 ttagcattaa acaaaggagt tttaaatatg attgaaatta cctatataga tgccagcaag
    11161 aacgaaagaa ctgtgacttt cgagtcttat gaagactttg aacgttcgca acaagcttgc
    11221 cttatcggcg tcgcagacta ctaccctgtc caaaaattaa cttacaaggg tcataatttg
    11281 gactaccatg ggacttatgg agatatcttc ttctatctca tgaaacaaga tttaagccaa
    11341 tataactaaa aaaaggagaa atacaatggc aaaagcaatt acagatgcaa cattcgaaca
    11401 agaaacaaaa gacggtttgg tcttagtaga cttctgggca acttggtgtg gtccatgtcg
    11461 tatgcaaggt ccaatcttgg acaaattgtc tgaagaactt tcagaagatg tcttgaaaat
    11521 cgttaaaatg gacgttgatg aaaatccaaa cacagctcgt gcttttggaa tcatgtctat
    11581 tccaactctt ctcttcaaaa aagacggcca agttgtcaaa caagttgcag gtgttcacac
    11641 agcagaacaa atcaaggcca tcattgctga attgagctaa tcaaacgaga gaccaagtac
    11701 tttctttggt ctcttttttc ttgccctttg ccatttttca aaaaatatgc tagactatag
    11761 tagattgaaa ctagaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttaac
    11821 tgtcctgatc gatttgtcct gttcttattt cattttacta tataagtaga atattacgat
    11881 gtttgggata tgccatcgct aaaaaaactc gatttggtat tttttagctc cctaatagga
    11941 gctttttgca tgctctgaac atttcccatt ttggaaggag tactatgaaa cgtcaatcag
    12001 ccttggtcgt ctttagtggt ggtcaagatt caacaacctg ccttttctgg gttatgcaac
    12061 actatgaaac agtcgaagct gtcacctttg cctacggcca acgtcatcac ctcgaaattc
    12121 aaattgctag agaaatcgct aaggaacagg gcattcgtca ccatatcctc gatatgtctc
    12181 tgctgggaca aatcactgct cagccagact ttgcgacgat tcatatttcc tacattcctg
    12241 acaagctctg tgtcgagtca aaatccctca aactatatct atttagctac cgaaatcacg
    12301 gagatttcca cgaaaactgt atcaacacca tcgggaaaga cttggtcaac ttgctagacc
    12361 ctcgctattt agaagtctgg ggaaaattca ctccgcgcgg tggcatttca atcgacccct
    12421 actacaacta cggtaagcaa ggaactaagt atgaggactt ggcagaacaa cgcctcttcc
    12481 aacacgacct ttatccagag aaaattgaca accgctaaag tcatactcaa tgaaaatcaa
    12541 agagcaaact aggaagttag ccgcaggttg ctcaaagcag tgtttgaggt tgtagata
//
LOCUS       DBERYS010000051        11990 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.051, whole genome shotgun sequence.
ACCESSION   DBERYS010000051 DBERYS010000000
VERSION     DBERYS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.051"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(83..1084)
                     /locus_tag="ACL8LA_001741"
                     /pseudo
     CDS             complement(83..1084)
                     /locus_tag="ACL8LA_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1380..1685
                     /locus_tag="ACL8LA_001742"
                     /pseudo
     CDS             1380..1685
                     /locus_tag="ACL8LA_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            1817..2761
                     /gene="manA"
                     /locus_tag="ACL8LA_001743"
     CDS             1817..2761
                     /gene="manA"
                     /locus_tag="ACL8LA_001743"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGS1813460.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(2783..4258)
                     /locus_tag="ACL8LA_001744"
     CDS             complement(2783..4258)
                     /locus_tag="ACL8LA_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HGS1813461.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            4341..5095
                     /locus_tag="ACL8LA_001745"
                     /pseudo
     CDS             4341..5095
                     /locus_tag="ACL8LA_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(5115..5570)
                     /locus_tag="ACL8LA_001746"
     CDS             complement(5115..5570)
                     /locus_tag="ACL8LA_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS1813462.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5590..6765)
                     /locus_tag="ACL8LA_001747"
     CDS             complement(5590..6765)
                     /locus_tag="ACL8LA_001747"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1813463.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFTGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(6824..7669)
                     /locus_tag="ACL8LA_001748"
     CDS             complement(6824..7669)
                     /locus_tag="ACL8LA_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1813464.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            7794..8351
                     /locus_tag="ACL8LA_001749"
     CDS             7794..8351
                     /locus_tag="ACL8LA_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1813465.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8370..8837
                     /locus_tag="ACL8LA_001750"
     CDS             8370..8837
                     /locus_tag="ACL8LA_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGS1813466.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8926..9555
                     /gene="upp"
                     /locus_tag="ACL8LA_001751"
     CDS             8926..9555
                     /gene="upp"
                     /locus_tag="ACL8LA_001751"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGS1813467.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            9729..10319
                     /gene="clpP"
                     /locus_tag="ACL8LA_001752"
     CDS             9729..10319
                     /gene="clpP"
                     /locus_tag="ACL8LA_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGS1813468.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            10398..10646
                     /locus_tag="ACL8LA_001753"
     CDS             10398..10646
                     /locus_tag="ACL8LA_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGS1813469.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            10747..11907
                     /locus_tag="ACL8LA_001754"
     CDS             10747..11907
                     /locus_tag="ACL8LA_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1813470.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         3764 a         2365 c         2505 g         3356 t
ORIGIN      
        1 tttaaaatag aaattgagaa tatgaactat tgaaagatcg gagcaatccg gtctttttat
       61 ataaagaaat tgcatataat actcatctgc tttcaaaaag cattctagtc catctccgat
      121 taacgatgga ctttatcacc tccttctcca gtccttgtat gacatcttga agttgattca
      181 tgacatcttc caaagttcga aaggccttat tcttaaatcc acgtttacga atctctttcc
      241 acacttgttc aatgggttca tctctggtgt gtatggagga ataaaggtaa aaccaatatt
      301 agtcggaatc tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaag
      361 ataatcatct ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc actcagtatt
      421 acatccacca gctattaaga aaaatgattc gcctgtatgg gcatcaacag ctccataaca
      481 atagtgaaat tctcgtatat agtgactatg gacatgtgga cctactccta ttggagccca
      541 acaagatccc agtttactga ttctaccgaa accagcctca tcttggtaca tcaagcgaac
      601 cttacgaaaa cgtctactgc ttttaaagcg ctttcttttc ttcttgaatt gagattttat
      661 ttttagacgc gacaatggtt tgagcgtctg ctttcctagg atgttctgga cgtggcataa
      721 tatttcgcca accatggtgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgac
      781 ctaactcctt tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg
      841 tagccttcaa atggcgggca agaaagactt tcttttcctc aatcgtcata tatgcatggt
      901 tacggccacc acgtgtttct tgaaggagag agtcgagttc gaactcctca tattttttta
      961 catttcgcca aatcgttgtt tgattacagt ctatagtgta ttgaatctat aacagtacac
     1021 cttagctgct aaaatatttc tataaattag tttgactttc ctgatagagt tgttcacatc
     1081 ttatttcaaa ctactataaa agttctataa tctctttata agatttgccc atcagacgaa
     1141 atataataaa ttgaaactag aatagtacac ctctacttct aaaacattgt tagaaatcga
     1201 tttgactgtc ctgatcgatt tgccctgttc ttgtttcgtt ttactataga acgatttgaa
     1261 ggcgtttatg atatttagct gtacgagagt ctttaaaaag tgttttgatg gtttggattt
     1321 cttctttagt tgatttcata tcactattat ataatgcttt ttgattttag tatagtatta
     1381 tcgtagaaaa tggactcggt gctatggata caccggatga gaatggttat gtagcagatg
     1441 actatcggat tacttactta gaggcccaca tcaaggccat gcgagatgcc atttacaaag
     1501 acggggttga cttgcttggt tatacgacat ggggctgtat cgattcggtt tcagctggca
     1561 caggggaaat gaataagcgc tatggtttta tctatgtaga tcgagataat gtaggaaatg
     1621 ggactctcaa acgtagcaag aagaagtcct tctattggta catgtcattt atagcaatgg
     1681 tgtaagcatc gagtagagca catttgttta ctatttttat aaaatcttct ccctgcttta
     1741 taagatgtaa aaaagagttc aattcgaact ctttttttgc tataatgagg ggagaaaaat
     1801 cagacaggag attaagatgt cagaaccatt atttttacaa tcagttatgc aagaaaaaat
     1861 ctggggtggt accaagctac gtgatgagtt tggctacgac atcccaagtg aaaaagtcgg
     1921 agagtactgg gctatctcag ctcatctaaa tggtgtttct aaagttgcca atggtcgtta
     1981 cgagggaaca gatcttgcta ctttgtatgc ggaacaccgt gaattatttg gcaatcgtcc
     2041 tgagcctgta tttccgcttt tgactaagat tttggatgct aacgactggc tcagcgttca
     2101 agttcaccca gacgatgctt atggactaga gcatgaaggc gaacttggaa aaacagaatg
     2161 ctggtacatc atcgctgcgg atgaaggttc agagattatc tacggccaca atgccaagtc
     2221 aaaagaagaa ctccgccagc aaatcgagga taagaactgg gatgcgctgt tgacaaaagt
     2281 gccagttaaa gcaggagatt tcttctatgt accaagcggt accatgcacg ctatcggggc
     2341 gggtatcttg attcttgaaa cccagcagtc tagcgatacc acttaccgtg tctatgactt
     2401 tgaccgtaag gatgacaagg gcaatttacg tgaacttcac cttgaaaaat ctatcgatgt
     2461 cttgaacatt ggtgaacccg caaatagccg tccagtgaca atcaaagcag atgatctacg
     2521 ttccactctc cttgtatcca atgatttctt cgcagtttac aaatgggaaa ttactggaaa
     2581 agttaacttt gaaaagacag ctgactacag cttgttgagt gtcttggctg gtcaaggtca
     2641 actaactgtt gacgggaaaa actatccaat ccaaaaaggg agacacttta tcctaccaag
     2701 tgatgttgaa gcttggactc tggaagggca agatttagaa ttaattgtta gtcatccata
     2761 aaaaagaaag ggcctgagtt cattactcaa gtcctttttg attacataaa ttgggcgatg
     2821 aagaccacga tgatgattgg aatgacggaa cgaagaaacg aagaaggaag agccagactt
     2881 ggaacagtcc ttgtttccat gctctttcat cgagatggag ttcctccatt gcaagagcct
     2941 ttttaaagat atagcctgta aaaagtgaaa ggtagagagc tccaaatggc atgaggagat
     3001 tgggaaccaa gaagttcata gcgtcaaaga aggtcttacc aaaaatgtga acatccgcca
     3061 tgacaccgta agatagggct gaaggaatgc caaagacaaa ggtcaaaatt cctaaaataa
     3121 cactccattt ggcacgtttg ctgttatcct ggttggtgat attgcccaca ttgatctcca
     3181 gcatcacgac agaagaagtg acagtcgcaa agaggaagag gacgtagaaa atggttccaa
     3241 aaggcatctt gtcaaagagt tgaggcaaga cgataaagag caagctcggt cccccttcag
     3301 actggatatt gaagggggac cgagcttgga aaatggctag acctgccatg atggatatcg
     3361 agatattcat ggctacgatg gagattcctg actggactag attggttttc ttgtccaagt
     3421 aagaagcata ggtcaacatg actgtaaccc ctagtgagag ggcaaagaaa gattgtccca
     3481 gagcatagag gagaccagcg ctagtcagtt ttgaaaagtc tggtttgagg aagtaaagaa
     3541 ccccttccat ggcatttggc aaactgagag agcgaccgat gataacaaca aagatgataa
     3601 agagcagggg catcatgact ttcgaagctc tttcaatccc tttttgaacc ccacgtgata
     3661 caataaagat atttaataag ataaaggctg cttgagctcc tagggcaatg gctggatttg
     3721 aaatgattga agtaaataac tgagcataat cacccgttcc accaagttgg aacaatttcc
     3781 caaactcaat acctagatag actagaatcc atcctccgat aacactgtaa aaagataaga
     3841 ggataaagag ggcaaaggcg ccaatccaac cgataaagtt gtacttgtta ttcttgccca
     3901 gttttccaaa ggttttgata gcggaaacgc cagcactacg gccaagggca aactcagcca
     3961 gcaggagagg gaaaccgatt aaaatagtgg aaatgagaaa gattagtaaa aagcctccac
     4021 cgccattagc agcagtcatg tagggaaact tccaaacgga accaagcccg atgaccgagc
     4081 cagcagatgc tagaataaag ccaagtttcg acccccattg tgatttttct gacatagtta
     4141 aactctgatc tcctttttca aaataagaaa aagctacttg agttgtcaaa tagccttctg
     4201 tttgattgat agacttgact atcctattat gttttctaag gtctgtcaag aaaacaattt
     4261 tcaagttttc acacctttct caaaaacgct tgactctgac ctaaggggag gggttatact
     4321 atcattgtaa ggaggaaatc atgtaccata taaaagaagc tgcgcagctt tcaggtgtct
     4381 ctgtcaagac cctgccctgc atcactatga caagatagga ctcttggtcc ccttaaagtc
     4441 ggaaaacggc tatcgaacct atagtcaaga ggatttggaa cgccttcagg tcattcttta
     4501 ctacaaatat ctaggctttt ctttagagaa aatagcagaa ctgttaaagg aagaaaggac
     4561 agatttattg ctccatttga ctaggtagtt ggactatcta actcgcgaaa ggcaacatct
     4621 ggataccttg atttccacct tgcaaaaaac tattcaagaa caaaaaggag aaagaaaaat
     4681 gaccattgag gaaaaattca cgggatttag ctatcaagac aatcaaaaat accaccaaga
     4741 agcggtagag aaatatggtc aagaagtcat gggacaagcg ctcgaacgcc aaaaaggtca
     4801 cgaagacgag gctacggccg cctttaacca agtctttcaa actttggcac aaaatcttca
     4861 agttggttta cctgcaacag caaccgaaaa ccaggagcaa gcagccaagc tcttgcaagc
     4921 cattcgcact tatggatttg actgctctat tgaggtattc ggtcatatcg gtaaaggtta
     4981 cgtctacaac ccagagttta aggaaaacat tgacaagttt ggttctgaaa cagcccagta
     5041 cacgtcagat gccattgcgg cttacgttca gacaaatgca gaaaaaatag gctaggaatt
     5101 tcctagccta ttttttactt caaatcataa agccagtcgt caccgttttt gtagtaaaag
     5161 aattcactga gatcttcttc tagaaacaca cgaagcatat cagacatatc atcggttgca
     5221 agttttagat gagaaagatt ttcaaagtcc tcccaccaaa ctttcccttc gtctgaagac
     5281 tggagttcac cagtaaagtg ttctgtcttg taaaaaagga cgacataacg ataatccttg
     5341 tcgtcatacc agtttttgat accacagagt tggggtttgg aaatgatcag accagtttct
     5401 tctttcactt cacgaatgac agcatcgaca aaggattcgc cacgttcaac atgaccacca
     5461 ggaaaagtaa tgccagacca gtcgggacta actcggtctt ggaccaggac cttatctccg
     5521 tttttaatca tacacatgtt aacaaattcg actgcctctc ttctgttcat tcttcacaac
     5581 ctttaatctt taatcataat gcagacttcc cgccacccag ccggtacaga gggcagaagt
     5641 gatgttaaag ccacccgtgt gggcattgat atccataact tcgcctgtaa agtggaggcc
     5701 aggtaccagc ttactttcaa gggttttagg attgatttcc ttgagactga ctccaccctt
     5761 ggtaacaaag gactttgcaa gggacatttt tccagttaca ggaattttaa gttctttaat
     5821 ggactggaca agttgttctc gttccttttc agtcagttgt ttgacttttt caggatatcc
     5881 ttgtacaaaa aattcggcca agcgttctgg taacaaggtt tttaaagcgt ttttcaagga
     5941 tttttcccga ttttcttcta gaaatgtaac caagtccttc tcagaaagtt gaggcaaaac
     6001 atcgagtgag agaacctccc cacctttgac aaagctagac atgcgtaggg cagcaggacc
     6061 tgacaaacca aagtgggtaa agagtaaatc atgagtgatg acatgcttac cataacttag
     6121 ggtcacatcg tccagagaaa tcccttgtaa ggctttatgt ggaaaatctg ttaataaagg
     6181 actttcagca gcctcaagat cggtgatggt atgcttaaaa tggcgagcaa tctcgtgacc
     6241 aaaaccagtc gaaccagtcg aaggataaga cttaccacct gttgtgacaa tgagtttctc
     6301 acaagtgaag gtttgatccg ctgacttaag gacaaactgg tcatctactt ttttaacaga
     6361 aacgatttct atttgagtag caacttgacc acctagttcg gtgattttct tttccaaagc
     6421 ttcgataata gtccgagact tgtcactggc tggaaagacg cgtccgtggt cttcgacctt
     6481 aagtttaaca ccattttctg taaaaaagtt gatgatgtca tgattatcga actgggagaa
     6541 aacactgtaa agaaagcgtc cgtttccagg aattccagct agcaggttgt ctaagctacc
     6601 attgttggtc acattgcaac gtcccccacc agtcccagct aatttttttc caagtttccg
     6661 atttttttcg atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg tagccatcat
     6721 accagcaggt cccccaccga tgacaatagt atcaaaatgt ttcatagctc tattgtacca
     6781 caaaaaaaca agagatgatg gtcacctctt gtcaagaatg caattaatca atttcatagc
     6841 ccatcagcaa accgccctct tctgcataga aactgcagag accagaggtt ggtagaattt
     6901 taatatccgc ttgtgggaag gtttcacgga ttcgctctga gagctgttga caacattttt
     6961 cgttattgcg ttgggccatg acaatacggc caccagcata tccagctttt actaactcat
     7021 cataggcagc ttgaactgat ttctttgatc cccttgcttt ttgtagcaat tcgagagttc
     7081 cagtttcact agcttttccg accatacgaa tgttgagaag gccaacgacc gtaccgataa
     7141 gcttgctcaa acggccgttc ttcaccaagt tatcgacttt ggctaggaca aagagcaact
     7201 tagttttttc ttgataggca gtgatagctt caaccacttc ttcaaaagac aagccctggt
     7261 caatcaagtc attcaatttt tctacgagta ggtcaacttc accaccagca gataaactat
     7321 caatcacatg aatcttagtg tcaggatggt cttctagata aatattcttt gctagttgag
     7381 cactattgtg actgccagaa agggtacctg tgatggttac taggaaaatg tttttggcac
     7441 cttcaaatgc tcgcaaatag tcatctgggc ttggacaagc cgattttgaa gcttctgcag
     7501 ttgcatacat ggtttccatc atttggtcaa tatcgagact ggcgtcatca acaaagacct
     7561 gatcagctac ttgaatggtt aaggggacac ttacaaaggt tgtgttaata gctggtgttg
     7621 gcagttgacg ataatcacaa ccagagtcag caataatttt ccaagtcata gaaattctcc
     7681 atctttgtca gttatacatt gacaaaggtt ctgtcttttt ttacaattat atcatgaaaa
     7741 cccttgaaac aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat gttatgtctg
     7801 aacgtagaat ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa gaatccaatt
     7861 tattaaccag agaagccatt gaaacagccc tcttgcaact cttggaaaaa aaggagctga
     7921 ccaagattag tatttctgaa ttggtcaaac gtgcaggtgt ttcgcgtgcg gccttttatc
     7981 gcaattatga ctccaaagag gaaattttag agagcgtctt taaacgaact gtccacaata
     8041 ttatggaaca gctgcatcat tacgatttaa agacagacct ttatttggtt tgggttcacc
     8101 ttttccggga ggccagaaag gaagctagag tgattcaatt ggccttggat taccatctgg
     8161 aaaaaatctt tgtccaagcc atgcaggaat ttctagaaaa ataccatggg aaatcaaaag
     8221 gtgtcagctc ttatcttcat tccttctgga gctcagccat cgtctctgtc cttctaaaat
     8281 ggatcaagga tggcatgaag gtaccagctg aaaagattgc agatttaggt ttaccatttt
     8341 ttaaaaaata gagaaaaagg agaaaagaga tgactgaaaa aagactggcc tgggatgagt
     8401 attttgcagc ccaagcccta ctaattgcga atcgttccac ttgtaaacgt gctaaagtgg
     8461 gcgcgattct ggtaaaagat aataaggtta tttccactgg ttacaatggt tcggtgtcag
     8521 ggactgagca ttgtattgat cacgaatgtt tggtcattga aggccactgt gttcgcaccc
     8581 ttcacgctga ggtcaatgct atccttcaag gtgctgagcg tggtgttccc aaaggcttta
     8641 cagcctatgt aacccatttt ccttgtctca actgtacaaa acaattgctt caggtcggct
     8701 gcaagcgcgt ggtttatatc aaccagtacc gaatggacga ctacgcccaa tacctttatc
     8761 aagaaaaagg gacagaattg acccatttac cacttgagac agtacagaca gctcttaaag
     8821 aggctgatct aatgtaaaaa ttatcaaaaa taaatggctt agaaagattt ttaaaccgtt
     8881 ttttggtata ataagaagaa taaattgaaa gaaggaattc caaaaatggg aaaaattgaa
     8941 gttattaatc acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac agatacttct
     9001 acaaaagctt ttcgtgagct agtagatgag attgcaatgt tgatggggta tgaagtactt
     9061 cgtgatcttc cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac agttcaaaaa
     9121 caattggcag gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat cgggatggtt
     9181 gatggtctct tgagcttggt tccagctgct aaagttggcc acatcggtat gtaccgtgat
     9241 gaagaaacac ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat tgaccaacgt
     9301 caaattttta tagtagaccc aatgttggca acaggtggct cagcaatctt ggctgttgat
     9361 tctcttaaaa aacgtggcgc atcaaatatc aaatttgtct gccttgtatc tgctccagag
     9421 ggtgtaaaag cccttcaaga agctcatcca gatgtagaaa tctttacagc agccttggat
     9481 gaacgtttga acgaacacgg ttatatcgtt ccaggtcttg gagatgctgg agaccgcttg
     9541 ttcggtacaa aataagatcg aaaagaagaa tgacttggaa aagtatttcc agtcacgaaa
     9601 ggaggttggg tttttgtttc tgtctaatga aagcagagca aaaatttgac cttttttgac
     9661 caagatatta taatagtctt atcttcagtc atttgaccaa caaaattaaa actcaaaagg
     9721 agaaatgaat gattcctgta gttattgaac aaacaagccg tggagaacgt tcttacgata
     9781 tttactcacg tcttctcaaa gaccgcatca ttatgctgac aggtccggtt gaagacaata
     9841 tggctaactc tgttattgcc caattgcttt tcttggatgc ccaagatagt acaaaagata
     9901 tttaccttta tgtcaataca ccaggtggtt ctgtttcagc tggtttggca atcgtagata
     9961 ccatgaactt tatcaaggca gatgtccaaa ccattgttat gggaatggct gcatctatgg
    10021 gaacagtcat cgcatcaagt ggagcaaaag gcaaacgttt catgcttcca aatgctgaat
    10081 acatgattca ccaaccaatg ggcggtacag gtggtggtac ccaacaaact gatatggcta
    10141 tcgctgcaga acacttgctc aaaactcgta ataccttgga aaaaatcttg gctgaaaatt
    10201 caggtcagtc aatggaaaaa gtccatgcag atgcagaacg tgataactgg atgagcgccc
    10261 aggaaacact tgaatatggc tttattgatg aaattatggc caacaattca ttgaactaat
    10321 gatgatagaa ggcaaactcg actgggtttg ctttttttgg tataataggg agagatttct
    10381 tagaaagagg atttatcatg tttgaaaaag tcaatcgctc tggcttgatt atctatcttt
    10441 actataatcg tgatgccaaa aaactgcagg attatggaga tattacctat cattccaaga
    10501 aacatcgtta cttacaactc tatgttccaa ctcaagaagt ggagcaattg gtcggacgct
    10561 tgagcaagga aaaatttatt aaaaaagtta gggtttgtca tatccaagag ttggaaacac
    10621 cctttgtggg caatctttat cgataggaaa acgttatcat cgaaaaagtt caaggaaaac
    10681 attgacaatt ttctgataat tcggtatatt cttaacagac tatttaagaa ataaggagac
    10741 aaaaagatga agaaaaaatt tgccctatcg tttgtggcgc ttgcaagtgt agcacttctt
    10801 gcagcctgtg gagaagtgaa gtctggagca gtcaacactg ctggtaactc agtagaggaa
    10861 aagacaatta aaatcgggtt taactttgaa gaatcaggtt ctttagctgc atacggaaca
    10921 gctgaacaaa aaggtgccca attggctgtt gatgaaatca atgccgcagg tggtatcgat
    10981 ggaaaacaaa tcgaagtagt cgataaagat aataagtctg aaacagctga ggctgcttca
    11041 gttacaacta accttgtaac ccaatctaaa gtatcagcag tcgtaggacc tgcaacatct
    11101 ggtgcgactg cagctgcggt agcgaacgct acaaaagcag gtgttccctt gatctcacca
    11161 agtgcgactc aagatggatt gactaaaggt caagattacc tctttattgg aactttccaa
    11221 gatagcttcc aaggaaaaat tatctcaaac tatgtttctg aaaaattaaa tgctaagaaa
    11281 gttgttcttt acactgacaa tgccagtgac tatgctaaag ggattgccaa atctttccgc
    11341 gagtcataca agggtgaaat cgttgcagat gaaactttcg tagcaggtga cacagacttc
    11401 caagcagccc ttacaaaaat gaaagggaaa gactttgatg ctatcgttgt tcctggttac
    11461 tataatgagg ctggtaagat tgtaaaccaa gcgcgtggta tgggaattga caaaccaatc
    11521 gttggtggtg atggattcaa cggtgaggag tttgtacaac aagcaactgc tgaaaaagca
    11581 tcaaacatct actttatctc aggcttctca actactgtag aagtttcagc taaagctaaa
    11641 gccttccttg acgcttaccg tgctaagtac aatgaagagc cttcaacatt tgcagccttg
    11701 gcttatgatt cagttcacct tgtagcaaac gcagcaaaag gtgctaaaaa ttcaggtgaa
    11761 atcaaggata accttgctaa aacaaaagat tttgaaggtg taactggtca aacaagcttc
    11821 gatgcagacc acaacacagt caaaactgct tacatgatga ccatgaacaa tggtaaagtt
    11881 gaagcagcag aagttgtaaa accataatag aaaaatgttg aaatagggaa tgagcctttg
    11941 actcactccc tgtttcgata tttaatactc ttcgaaaatc tcttcaaact
//
LOCUS       DBERYS010000052        11887 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.052, whole genome shotgun sequence.
ACCESSION   DBERYS010000052 DBERYS010000000
VERSION     DBERYS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.052"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..144
                     /locus_tag="ACL8LA_001755"
     CDS             <1..144
                     /locus_tag="ACL8LA_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1813471.1"
                     /translation="KERSKVENIFAKVKTFKIISTTYRNHRKRFGLRMNLSAGIINHE
                     LGF"
     gene            335..2626
                     /gene="pcrA"
                     /locus_tag="ACL8LA_001756"
     CDS             335..2626
                     /gene="pcrA"
                     /locus_tag="ACL8LA_001756"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGS1813472.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2669..3349
                     /gene="radC"
                     /locus_tag="ACL8LA_001757"
     CDS             2669..3349
                     /gene="radC"
                     /locus_tag="ACL8LA_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS1813473.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3346..4035)
                     /locus_tag="ACL8LA_001758"
     CDS             complement(3346..4035)
                     /locus_tag="ACL8LA_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS1813474.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4053..4694)
                     /locus_tag="ACL8LA_001759"
     CDS             complement(4053..4694)
                     /locus_tag="ACL8LA_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGS1813475.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4777..4992)
                     /locus_tag="ACL8LA_001760"
     CDS             complement(4777..4992)
                     /locus_tag="ACL8LA_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS1813476.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5000..5347)
                     /locus_tag="ACL8LA_001761"
     CDS             complement(5000..5347)
                     /locus_tag="ACL8LA_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGS1813477.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5352..6467)
                     /locus_tag="ACL8LA_001762"
     CDS             complement(5352..6467)
                     /locus_tag="ACL8LA_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS1813478.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6477..7436)
                     /locus_tag="ACL8LA_001763"
     CDS             complement(6477..7436)
                     /locus_tag="ACL8LA_001763"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS1813479.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7549..8118)
                     /locus_tag="ACL8LA_001764"
     CDS             complement(7549..8118)
                     /locus_tag="ACL8LA_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGS1813480.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8246..8917
                     /locus_tag="ACL8LA_001765"
     CDS             8246..8917
                     /locus_tag="ACL8LA_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGS1813481.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8901..9719
                     /locus_tag="ACL8LA_001766"
     CDS             8901..9719
                     /locus_tag="ACL8LA_001766"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGS1813482.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9716..10612
                     /locus_tag="ACL8LA_001767"
     CDS             9716..10612
                     /locus_tag="ACL8LA_001767"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1813483.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10656..11630
                     /gene="pta"
                     /locus_tag="ACL8LA_001768"
     CDS             10656..11630
                     /gene="pta"
                     /locus_tag="ACL8LA_001768"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGS1813484.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3468 a         2276 c         2563 g         3580 t
ORIGIN      
        1 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aataatttca
       61 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgag tgctggtatt
      121 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttgaggtatt gagctagttt
      181 atgaaaaaat tgggtgaaaa gtcgagtgtt ttagaaaccc acagtgtagt attctagttt
      241 caatccacta tattttgcta ctccccgtaa agtttctatt ttccctgatt tctgatataa
      301 tagaaatatt gacttcaaga gtaaggaaga gaagatgaac gcattattaa atggaatgaa
      361 tgaccgtcag gctgaggcgg tgcaaacgac agaaggtccc ttgctaatca tggcaggggc
      421 tggttctgga aagactcgtg ttttaaccca ccgtatcgct tatttgattg atgaaaagct
      481 ggtcaatcct tggaatatct tggccattac ctttaccaac aaggctgcgc gtgagatgaa
      541 agagcgtgct tatagcctca atccagctac tcaggactgt ctgattgcga ccttccactc
      601 catgtgtgtg cgtattttgc gtcgcgatgc ggaccatatt ggctacaatc gtaattttac
      661 aattgtggat cctggtgaac agcgaacgct catgaaacgt attctcaaac agttgaactt
      721 ggatcctaaa aaatggaatg aacgaactat tttggggacc atttccaatg ctaagaataa
      781 tttgattgat gatgttgctt atgctgccca agctggcgat atgtatacgc aaattgtggc
      841 ccagtgttat acagcctatc aaaaagaact tcgtcagtct gaatccgttg actttgatga
      901 tttgattatg ctgaccttgc gtctctttga tcaaaatcct gatgttttga cctactacca
      961 gcaaaaattc caatacatcc acgttgatga gtaccaagat accaaccacg ctcagtacca
     1021 attggtcaaa ctcttggctt cccgttttaa aaatatctgt gtggttgggg atgcggacca
     1081 gtctatctac ggttggcgtg gtgctgatat gcagaatatc ttggactttg aaaaggatta
     1141 ccccaaagcc aaggttgtct tgctggagga aaattaccgc tcaaccaaaa ccattctcca
     1201 agcggccaac gaggttatta aaaataataa aaatcgccgt cctaaaaatc tctggactca
     1261 aaacgctgat ggggagcaaa tcgtttacta tcgtgccgat gatgagctgg atgaggctgt
     1321 atttgtagcc agaaccatcg atgaacttag tcgcagtcaa aacttccttc ataaggattt
     1381 tgcagttctc tatcggacta atgcccagtc ccgtacaatt gaggaagccc tgctcaagtc
     1441 taacattcct tataccatgg ttggcggaac caaattctac agccgtaagg aaattcgcga
     1501 tattattgct tatctcaacc ttattgctaa tttgagtgac aatattagtt ttgagcgtat
     1561 tatcaacgag cctaaacgtg gaattggtct aggtacagtt gagaaaatcc gtgattttgc
     1621 aaatttgcaa aatatgtcta tgctggatgc ttctgctaat attatgttgt ctggtatcaa
     1681 gggtaaggca gcccaatcta tctgggattt tgccaatatg atgcttgatt tgcgggagca
     1741 gctagaccac ttaagcatta cagagttggt tgagtccgtc ctagaaaaaa caggttatgt
     1801 cgatattctt aacgcccaag cgactctaga aagcaaggca cgggttgaaa atatcgaaga
     1861 gtttctttct gttacgaaga actttgatga caccacggat gtgacagaag aggaaactgg
     1921 tctggacaaa ctgagtcgtt tcttaaatga cttggctttg attgccgaca cagattcagg
     1981 tagtcaggag acatcagaag tgaccttgat gaccctgcat gctgccaaag gtctcgaatt
     2041 tccagttgtc tttttgattg ggatggaaga aaatgtcttt ccacttagtc gtgcgactga
     2101 agatccagat gaattagaag aagagcgccg tctagcctat gtaggtatca cgcgtgcaga
     2161 gaaaattctc tatctgacca atgccaactc acgcttgctt tttggtcgta ccaattataa
     2221 ccgtccgact cgttttatta acgaaatcag ttcagacttg cttgagtatc aaggtctggc
     2281 tcgtcctgca aatacaagct ttaaggcatc atatagcagt ggtagtattt cctttggtca
     2341 aggtatgagt ttggctcagg ctcttcaaga ccgtaaacgc ggtgctgccc caaaatcaat
     2401 ccagtcaagc ggtcttccat ttggtcaatt tacagctggc gcaaaaccag catctagcga
     2461 ggcaaattgg tccattggtg atattgctct ccacaagaaa tggggagagg gaaccgttct
     2521 ggaagtttca ggtagcggtg ctaggcagga attgaaaatc aatttcccag aagtaggttt
     2581 gaaaaaactt ttagccagtg tggctccaat tgagaaaaaa atctaatttt ccatccttct
     2641 cacgaataat aaagtgagga ggatttttat gtacagtatt tcattccaag aagattcact
     2701 attaccaaga gaaaggctgg ccaaggaagg agttgaagcg cttagtaacc aagagttgct
     2761 agctatttta ctcaggacag gaacacgtca agttagcgtt tttgaaattg cccaaaaagt
     2821 cttgaacaat ctttcaagcc taacggattt gaaaaaaatg accctgcagg aattgcagag
     2881 tttgtctggt attgggcgtg ttaaggccat agaattacaa gctatgattg aactggggca
     2941 tcgtattcac aaacacgaga ctcttgaaat ggaaagtatt ctcagcagtc aaaagttggc
     3001 caagaagatg cagcaggaat taggggataa aaaacaagag cacctggtgg cactctatct
     3061 caatactcaa aatcaaatca tccatcagca gaccattttt atcgggtctg taactcgtag
     3121 tatcgctgaa ccgcgagaga ttcttcacta tgcaatcaag catatggcga cttctcttat
     3181 cttggtccac aatcatcctt caggagcggt agcgcctagc caaaatgatg atcatgtcac
     3241 taaacttgtt aaagaagcct gcgaattgat ggggattgtt ctcttggacc atttgattgt
     3301 ctctcattct aattacttta gttatcgtga aaagacagat ttaatctaaa gttcattaac
     3361 gacatagtca aagagttttt tatctttggg acgattttca aaaagaagtt ctggatgcca
     3421 ttggacaccg agaaaggcga catcatccgt actcatgaca gcctcaatga taccatcttt
     3481 aggatcatga gccacaactt ttaaatttgg tgctaagtcc ttgatgctct ggtggtggaa
     3541 ggagttgata tgagagattt ctccatagat ttcttggaga acggtatctg gttctgttac
     3601 caagcgttga gttgtgtact caacagaaga atcctgccaa tggtcttcga tatcttggta
     3661 caaagttcca cccatggcaa cgttaaagag ttgggtacca cggcagacag agaaaatggg
     3721 ctttttctgt ttaatagctt ccttgatgag ggtcagttcg aagatatctc tttgaaggtg
     3781 atagtcatcg ctatcaatgg ttttgggttc gccataaaat tttggatcga cattttgccc
     3841 acctgtcaag atgagcttgt caatcaaact gatatagtgg caggccattt cttgatcacc
     3901 aatcggtagg atgatgggaa tccctccagc atctttaacg ccttcaacaa agccttttgc
     3961 tgcgtagctc atcatgatgt catcatctgg atgagctttt tcgtttcctg taatcccaat
     4021 aactggtttt ttcataaaat gattttcgct ttctaatcct cttttcgcat gaagtagagg
     4081 agggtttgga gttcacttgt caaatcgaca tactgaacga ccacgtcttt tggtaaatgc
     4141 agatggactg gtgaaaaact gagaattcct ttcacaccag catcaaccaa gagattagca
     4201 acctcttgtg acttgacgct gggaacagtt aggatagcag tcttcacatc agcatccttg
     4261 attttatcct tgatctgaga aatcccgtaa atgggaatcc cgtcaggagt ttgggtaccg
     4321 acttcaggat ggtcgtctag gtcaaaggcc atgataatct tcatcttgtt acgttcgtgg
     4381 aagcggtagt ggagaagggc atggcccata tttccaatac caaccagcat gacattggta
     4441 atagagttgt cattgagcaa atcggcaaaa aatgtcatta gttttttgac atcatagcca
     4501 aaaccacgac gaccaagttc accaaaatag gaaaaatcac gacgtacggt cgctgaatca
     4561 ataccgatag cctctgcaat ttgcttagag ttggcacgtt caatcttttc tgcatgaaat
     4621 ctcttaaaaa ttcgatagta gagagagagt ctttttgctg tagcttttgg aatagcaaac
     4681 tgtttatctt tcacaaaatc acaacctttc tattcttcta ttttatagaa acattgtgaa
     4741 aaaatcaaca aaaataagaa aaaactaaga aaaatcttag ttttgatgta aaaaatctgc
     4801 atgagataga aaacggtaga ggtctccgac cagaccctga taaacttttt ggcccctaaa
     4861 agtcagagaa gtcacataaa gtgtatctgg taaggttaca catcctgaca aagtcaacat
     4921 gagagcctca tgatcctcat acttgagagt acgctctaca tgatagcagt ccttataggt
     4981 cagttcaaac attttggctc tatctttccg attttgtaaa gacaccacgt tctaccaagc
     5041 tatccatgag gaagtagaat ttttcctgat gaatatggtg gtcttctgat ttgaaaatat
     5101 caactagacg aaggccaaac ttgtcagtga tattgatttt agcccctgta agttccttgt
     5161 taatgatgat tttgagttgg aagccttcac cgctgtttgg cactttttcc aaaaggcgag
     5221 tcagttcata gttaccaacc ttagtttcaa aaaaggtgtt atctttgagg gtgaattttt
     5281 taacagaagg gctaagagtg taatcgtaac gacaattttt taactgaatg attttttcaa
     5341 atgccatatg gctaacctcc gataatttct tttaaggttt ttgcgagggt ttgtaggtct
     5401 tcaacggtat tttgtggcga caaactgatg cgaagggatt ccttcaagcg ttctgaattt
     5461 gcgccataca tggcttcaag aacatggctg gattggacaa cgcctgcagt acaggctgag
     5521 ccagtagaga ttgaaattcc agctaaatct agccgaagga gtaagaggtc atttttctga
     5581 ccaggaaatc caatattgag aacataaggg agatgatgtt ttcctctatt caagtaatac
     5641 tgaatgccct ccagctctgc cagaaaggca gtttctagat tttgtacatg ttgaaaatgt
     5701 tcttcttgtt tttctaggtc ttcttttagg gctgcaacca tgcctacgat agcaggcaga
     5761 ttttcagttc ctgcacgttt tttctgttcc tggtctccgc catgtagata ggaatcaaag
     5821 tccatgctag atgcgtagag aaaaccgatt cccttaggac catggaattt gtgggcagaa
     5881 gcagtgagaa aatcaatgcc caattcttct gaatgaattg ggattttacc aatagcctga
     5941 actgcatcaa catgataggc agcagggtgt tgcttgagta tttggccaat ttcagcgatg
     6001 ggcagtaggt ttcctgtctc attattggca aacatggtag aaaccaaaat cgtatcgtca
     6061 cgtaaagcct tttgaatttg ctgggctgtg atttcttgat tttctggctg gataatggtt
     6121 gcttcaaacc caaagtgttg aaccaagtaa tcaattgttt caaggacagc atggtgctcg
     6181 atggcagttg tgatgatatg ttttccttgt tcttggtgac gaagacagta gccaatgatg
     6241 gtagtattat tgccttcagt cccaccagaa gtgaaaaaga tatgttgagg ttttgtcctt
     6301 agtaactggg ctagttcctg acgggcttct cgcaagagtt tgccagcttg acgaccatga
     6361 ccatgaatac tagaaggatt tccgtgggtt tcttgcataa ccttggtcat agctgaaata
     6421 gcaactgctg acataggagt cgttgcagca ttgtccaaat aaatcaaaga atcaccttat
     6481 ttctttttat tgtaggcaaa gagtgggctg actggttttc tttcgtgaat acggacgata
     6541 gcatcaccaa ttaactcact agcagtgatg tagcatacat ttttaggagt tttttctttt
     6601 gttgctactg aatcagtcac aagaatttct ttaatattag tattgtcaag aagctcagca
     6661 gctccctcga cgaagagacc gtggctagaa acagcataaa tttctgtagc tccttcacgt
     6721 tcaacgattt tagaagcttc agagaaggta cgtcctgtat ttaaaatatc atcaatcaag
     6781 atagctttct taccttcaac atcaccaata atataacctt cgttacgagt tgcatcgtct
     6841 tgagggtagt cgataatggc gataggagca tcaagatatt cagccaggct acgcgcacgt
     6901 ttgacacctg aatttttagg gctaacgaca acaacatctg aaccaagcaa tcctttatcg
     6961 cagtaatgtt ttgcgaatag gggaacagtg aaaagattat ccactggaat atcaaagaaa
     7021 ccttgaacct gaacggcatg caaatcaaga gtcaggatac gatcaactcc agccttaacc
     7081 agcatattgg caactagttt tgctgtaagt ggctcacgag gacaagcaat gcggtcttga
     7141 cgtgcatagc caaaatatgg aaggacaacg ttgatactgt gggcacttgc acgcacacaa
     7201 gcatcgacca tgattaacaa ttccattagg tggttgttga cagggaaact tgttgattgg
     7261 atgatgtaaa catcataacc acggacactt tcttcgatat ttacttggat ttctccgtct
     7321 gaaaattgac gtgatgatag ttttccaagt gggacaccaa cagcttgggc aattttttgt
     7381 gcaatctctt ggttagagtt gagtgcgaaa agtttcatgt tttttctatc tgacattata
     7441 gaccgtcctc tgtaaacttt ataaatccta gttatattta ccttacatat atgaactggg
     7501 atttgtgtat ttttatcttt tctattttac caaaaaatgg agattatttc agctattttt
     7561 catacttttg acaaatcgaa ccaattttga aggagctttt tggtaggaaa tctgattttt
     7621 ctctaaaaat tgtcggaaat cctgtttgcc ttgctcatga ttttccactt caagctccaa
     7681 ttcgtaatct gttatatcaa agtatcggct ctgatccagt gccatgagac caatagctgt
     7741 tttcatttca tagcgaagcg ttgttagaca accaagaacc tgccagttct tactttggat
     7801 accatgtttc gccaattcat ccagtactag cccttgagga agttcttcct tactcagata
     7861 gttctcagca tcttttagtt gcaatttttg gttgtattcc atgtttccaa cactctgcgg
     7921 gactttgagt gtcaactcag cccagtcttc aaaggttcga atgcgcatag cgactttctt
     7981 ttctcgcagt tcaaaatcag gcgtgtcgat gtagtaattt gtttgaagaa caggagtgac
     8041 acctgtgaac tggtctttta gacgattgta ttcatctttt ttcaatagtg ttttcaattc
     8101 aatttctaaa tgtttcattt ttcttacctt tttttatcgt tgaaagcgga tttatggtat
     8161 aataagcatt gtatttattg tatatgaatc tggagaaaaa atcaaagata tttttgacgg
     8221 ataatatgag aacaagggag aatatatgac cttagaatgg gaagaatttc tagatcctta
     8281 cattcaagct gttggtgagt taaagattaa acttcgtggt attcgtaagc aatatcgtaa
     8341 gcaaaataag cattctccaa ttgagtttgt gaccggtcga gttaagccaa ttgagagcat
     8401 caaagaaaaa atggctcgtc gtggcattac ttatgcgacc ttggaacacg atttgcagga
     8461 tattgctggc ttacgtgtga tggttcagtt tgtagatgac gtcaaggaag tagtggatat
     8521 tttgcacaag cgtcaggata tgcgaatcat acaggagcga gattacatta ctcatagaaa
     8581 agcatcaggc tatcgttcct atcatgtggt agtagaatat acggttgata ccatcaatgg
     8641 agttaagact attttggcag aaattcaaat tcgtactttg gccatgaatt tctgggcaac
     8701 gatagaacat tctctcaact acaagtacca aggggatttc ccagatgaga ttaagaagcg
     8761 actggaaatt acagctagaa tcgcccatca gttggatgaa gaaatgggtg aaattcgtga
     8821 tgatatccaa gaagctcagg cactttttga tcctttgagt agaaaattaa atgacggtgt
     8881 aggaaacagt gacgatacag atgaagaata caggtaaacg aattgatctg atagccaata
     8941 gaaaaccgca gagtcaaagg gttttgtatg aattgcgaga tcgtttgaag agaaatcagt
     9001 ttatactcaa tgataccaat ccggatattg tcatttccat tggcggggat ggtatgctct
     9061 tgtcggcctt tcataagtac gaaaatcagc ttgagaaggt ccgctttatc ggtcttcaca
     9121 ctggacattt gggcttctat acagattatc gtgattttga gttggacaag ctagtgacta
     9181 atttgcagct agatactggg gcaagggttt cttaccctgt tctgaatgtg aaggtctttc
     9241 ttgaaaatgg tgaagttaag attttcagag cactcaacga agccagcatc cgcaggtctg
     9301 atcgaaccat ggtggcagat attgtaataa atggtgttcc ctttgaacgt tttcgtggag
     9361 acgggctaac agtttcgaca ccgactggta gtactgccta taacaagtct cttggcggtg
     9421 ctgttttaca ccctaccatt gaagctttgc aattaacgga aattgccagc cttaataatc
     9481 gtgtctatcg aacactgggc tcttccatta ttgtgcctaa gaaggataag attgaactta
     9541 ttccaacaag aaacgattat catactattt cggttgacaa tagcgtttat tctttccgta
     9601 atattgagcg tattgagtat caaatcgccc atcataagat tcactttgtc gcgactccta
     9661 gccataccag tttctggaac cgtgttaagg atgcctttat cggtgaggtg gatgaatgag
     9721 gtttgaattt atcgcagatg aacatgtcaa ggttaagacc ttcttaaaaa agcacgaggt
     9781 ttctaaggga ttgctggcca agattaagtt tcgaggtgga gctattctgg tcaataatca
     9841 accgcaaaat gcaacgtatc tattggacgt tggagactac gttaccattg acattcccgc
     9901 tgagaaaggc tttgaaacct tggaggctat tgagcttcca ttagatattc tctatgagga
     9961 tgaccacttt ctagtcttga ataaacccta tggagtggct tctattccta gtgttaatca
    10021 ctctaatacc attgccaatt ttatcaaggg ttactatgtc aagcaaaatt atgaaaatca
    10081 gcaggttcac attgttacca gactagatag ggacacttct ggcttgatgc tctttgccaa
    10141 gcacggttat gcccatgcac gattagacaa gcagttgcag aagaaatcta tcgagaaacg
    10201 ctactttgct ttggttaagg gagatggaca tttggaacca gaaggggaaa ttattgctcc
    10261 gattgcgcgt gatgaagatt ccattattac cagacgagtg gctaaaggcg gaaagtatgc
    10321 ccatacttca tacaagattg tagcttctta tggaaatatt cacttggtct atattcacct
    10381 gcacactggt cgaacccatc aaatccgagt ccatttttct catatcggtt ttcctttgct
    10441 gggagatgat ttgtatggtg gtagtctgga agatggtatt caacgtcagg ctctgcattg
    10501 ccattaccta tccttttatc atccattttt agagcaagac ttgcagttag aaagtccctt
    10561 gccggatgat ttcagtaacc ttattaccca gttatcaact aatactctat aaaaactgtc
    10621 tcagagtata attattatct taaaggagaa aactcatgga agtttttgaa agtctcaaag
    10681 ccaaccttgt tggtaaaaat gctcgtatcg ttctccctga aggggaagag cctcgtattc
    10741 ttcaagcaac aaaacgctta gtaaaagaaa cagaagtgat tcctgttttg cttggaaatc
    10801 ctgaaaaaat taaaatttat cttgaaattg aaggaatcat ggatggttat gaggtcatcg
    10861 accctcaaca ttatcctcaa tttgaagaaa tggtttctgc cttggtggag cgtcgcaagg
    10921 gcaaaatgac tgaagaagat gtacgcaagg ttttggttga agatgtcaac tactttggtg
    10981 tgatgttggt ttacttgggc ttggttgatg gaatggtgtc aggagcgatt cactcaacag
    11041 cttcaacagt tcgcccagct ctacaaatca tcaaaactcg tccaaatgta actcgtactt
    11101 caggagcctt cctcatggtt cgtggtacgg aacgttacct atttggagac tgtgccatta
    11161 atatcaatcc agatgcagaa gccttggctg aaattgccat caactcagca atcacagcta
    11221 agatgtttgg catcgaacct aaaattgcca tgttgagcta ttctactaaa ggttcagggt
    11281 ttggtgaaag cgttgataag gtcgttgaag caactaaaat tgctcacgac ttgcgtcctg
    11341 accttgaaat cgatggtgag ttgcaatttg atgcggcctt tgttcccgaa actgcagctc
    11401 tgaaagctcc gggaagtaca gtagctggtc aagcaaatgt cttcatcttc ccaggtatcg
    11461 aggcaggaaa tatcggttac aagatggctg aacgcctggg tggctttgcg gctgtaggac
    11521 ctgttttgca aggtttaaac aagccagtta atgatctttc tcgtggatgt aatgcagatg
    11581 atgtttacaa gttgaccctc atcacagcag ctcaagcagt tcatcaatag tgaaaactat
    11641 aaagtgatat actatgctat actgtagtta tgaaactatg tacgaaaagc actgccatta
    11701 attcctgaga actaaattac tgattggtgt caaaaaggaa aacttccaag cgatgatatc
    11761 ctgtctatac acgacctata gaaatctgta atatacatat ccgtaaaacg ataaattccc
    11821 tttttgattt taaatgagta tgaaaagaga attttttggc tctttgtcaa ctgtagtggg
    11881 ttgaagt
//
LOCUS       DBERYS010000053        11615 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.053, whole genome shotgun sequence.
ACCESSION   DBERYS010000053 DBERYS010000000
VERSION     DBERYS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.053"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(141..992)
                     /gene="rgg"
                     /locus_tag="ACL8LA_001769"
     CDS             complement(141..992)
                     /gene="rgg"
                     /locus_tag="ACL8LA_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HGS1813485.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNVAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFYTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            1116..1457
                     /locus_tag="ACL8LA_001770"
     CDS             1116..1457
                     /locus_tag="ACL8LA_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS1813486.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            1521..1685
                     /locus_tag="ACL8LA_001771"
     CDS             1521..1685
                     /locus_tag="ACL8LA_001771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813487.1"
                     /translation="MKIKGQTRKLAAGCSKHCFEVMDRTDEVSPKHCFEVVDRTDEVS
                     NHTYGKATLT"
     gene            complement(1711..3414)
                     /gene="ilvD"
                     /locus_tag="ACL8LA_001772"
     CDS             complement(1711..3414)
                     /gene="ilvD"
                     /locus_tag="ACL8LA_001772"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGS1813488.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(3647..4579)
                     /locus_tag="ACL8LA_001773"
     CDS             complement(3647..4579)
                     /locus_tag="ACL8LA_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGS1813489.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(4576..5433)
                     /locus_tag="ACL8LA_001774"
     CDS             complement(4576..5433)
                     /locus_tag="ACL8LA_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1813490.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(5437..6783)
                     /locus_tag="ACL8LA_001775"
     CDS             complement(5437..6783)
                     /locus_tag="ACL8LA_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1813491.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(6796..7080)
                     /locus_tag="ACL8LA_001776"
     CDS             complement(6796..7080)
                     /locus_tag="ACL8LA_001776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1813492.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(7084..9114)
                     /locus_tag="ACL8LA_001777"
     CDS             complement(7084..9114)
                     /locus_tag="ACL8LA_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1813493.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEYYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            9305..10312
                     /locus_tag="ACL8LA_001778"
     CDS             9305..10312
                     /locus_tag="ACL8LA_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS1813494.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            10315..10515
                     /locus_tag="ACL8LA_001779"
     CDS             10315..10515
                     /locus_tag="ACL8LA_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1813495.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            10667..10849
                     /gene="rpmF"
                     /locus_tag="ACL8LA_001780"
     CDS             10667..10849
                     /gene="rpmF"
                     /locus_tag="ACL8LA_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGS1813496.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            10865..11014
                     /gene="rpmG"
                     /locus_tag="ACL8LA_001781"
     CDS             10865..11014
                     /gene="rpmG"
                     /locus_tag="ACL8LA_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1813497.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(11506..>11615)
                     /locus_tag="ACL8LA_001782"
     CDS             complement(11506..>11615)
                     /locus_tag="ACL8LA_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS1813498.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3759 a         2443 c         1912 g         3501 t
ORIGIN      
        1 tagcacttat gaatttgcga aacgattctt ttgaatatat gcaacaaaca aaatgagcaa
       61 aaaaacgata gaacttttgt aatgacctga gagagggcaa cagttctatc gcttcaaatt
      121 ttttcttggt ttgcagatac tcaacattcg gtcgtatttc tatagtattc ggcagattta
      181 ttacagccaa gcatctcaaa aatacggaca gcatcctcca tctttttctg accttccttg
      241 actctacctt gcttgctatc aaggagacct tctgcccaca gataaacaat tcggaaatag
      301 gtctcatttt ccttgtagaa atgctcttcg ataacacgtt taaaataata ggcattggta
      361 aattcttcac actcaatact agctaaaaag ccattcaata gtatagtata aaaaaggttt
      421 cgattgccag acatttccat tagaaaatcg gatttacgta ccatttctcg tacatatcta
      481 gtaaaaagag aaacagataa aaatggagaa ctgactgaaa ataaattgag ttcatagatt
      541 ccccagatct cggtagaaaa caaataatca tgaaggactt ttccttcctc tgctgttaac
      601 tctacccttt catccatgct cttcatataa gacttgatca taatggcatt tagaatatgt
      661 ttctgtttgt tgtgagaatg ggcatgcttt tgatactccc tgcgatataa gtcctcaaga
      721 ggtgctacat tctttggttc caagacatct gtaatttctt ttctcaactc agaatctgta
      781 tcatactgga aacctctcgc cagaaagagg atctcctcca cactggcaga tatattttcc
      841 agagcaaata gaaacttttc caccgaaagc tcactctgac ctgtttcaaa acgggacaac
      901 atagacggcg aaaattgtcc tccggttgct tgtctcagtg agatatttct tgactctcgt
      961 aattgtctaa agacttttcc aatctgctcc atagacttcc ccttgattcc gtattttctt
     1021 cattttatca tatttttcag aaaattcatc aaaaacttgc caaattgtca gaattatgag
     1081 aaaatagagg atatttatca cgtggaggga ctgctatgag agacgatatc aaaatcaatg
     1141 accgtgcttt ggccttgcaa gaccaaatca tcgaaaaact agagaaagtt tttgatacag
     1201 atgtggaatt ggatgtttac aatctaggtc tgatttatga aatcaatctg gatgaaacgg
     1261 ggctctgcaa gattgtcatg accttcaccg atactgcctg tgattgcgcc gaaagcctgc
     1321 ctattgaaat cgtggcaggt ctgaaacaaa tcgagggtat caaagatatc aaggttgaag
     1381 ttacctggtc gcctgcttgg aaaatcacac gaatcagtcg ctatggccgt attgcccttg
     1441 gactaccacc tcgttaagca ggccaatcat ttttaaagat gaaaaaagca agccaaaaat
     1501 ggcttgcttt ttgatactca atgaaaatca aagggcaaac tagaaaacta gccgcaggtt
     1561 gctcaaaaca ctgttttgaa gttatggata gaactgacga agtcagccca aaacactgtt
     1621 ttgaggttgt ggatagaact gacgaagtca gtaaccatac ctacggcaag gcgacgttga
     1681 cgtgatttga agagattttc aagtatgagt ttatttttta cctgacttgt ccatattcca
     1741 gaagtctgtc acggctccgc gtgaagcaga tgatacgata tgggcatatt taccgaggac
     1801 accacggctg taaagtggtg gcaaggttgt ttctgccttg cgtttttcaa gttcttcttc
     1861 ggatacggcc atggaaattt ctttggtatc ttggtcaacc gtaacgatat cgccggtacg
     1921 gagataggca attggtccac catcctgagc ttcaggagcg atatgtccaa caaccagacc
     1981 ataagtacca ccagagaaac gtccgtccgt caagagggcc accttatctc cctgaccttt
     2041 accaacaatc attgaagaaa gtgacagcat ctcaggcata ccaggaccac ctttaggtcc
     2101 aacaaaacga acaacgacta catcgccatc aacgatttca tctgtcagaa cggcctgaat
     2161 cgcatcttct tctgagtcaa agaccttagc tggcccaacg tgacgacgca ctttaacacc
     2221 tgataccttg gcaactgcac cgtcaggagc aaggttcccg ttcaagatga taagcggacc
     2281 atccgcacgt tttggatttt caagtggcat gataactttt tggcctggag tcaagtctgc
     2341 aaagtcagcc aagttttcag ctacagtctt accagtacat gtgatgcgat ctccgtgaag
     2401 gaaaccattt gccaacaagt acttcataac cgcagggaca ccaccgactt cgtagaggtc
     2461 ttggaagaca tactgaccag atggtttcaa gtcggccaag tgaggcacac gttcttgaat
     2521 cgtattgaag tcctcaagtg acaagtcaac atttgcggca tgggcaatgg cgagcaagtg
     2581 aagagtggcg tttgtagaac caccgagagc catcgttaca gtaatagcat cttcaaaggc
     2641 ttcacgagtc aagatatctg atggtttgag accaagttcc aacatcttaa caacagcacg
     2701 tcctgctgct tcgatatctt ctttcttatc agctgattca gctgggtgag aggatgaccc
     2761 tggcaaactc atccctagaa cttcgatagc agttgccatg gtattagcag tatacatacc
     2821 accacaacca ccagggccag ggcaggcatt acattcaaga cgtttcacgt cctcagctgt
     2881 catgtcaccg tggttccatt ttccgatacc ttcaaagaca gaaaccaagt cgatatcttt
     2941 accatcaaga tttcccggtg caatagttcc accataggcg aaaatagctg ggatatccat
     3001 attagcaata gcaatcatag atccaggcat gttcttgtca cagccaccga tagcgacgaa
     3061 ggcatccacg ttgtgaccac tcatagccgc ctcgatggag tccgcgatga tgtcacgaga
     3121 tgttagagag aaacgcatac caggcgttcc catagcgatc ccgtccgcta cggtaatggt
     3181 tccaaactgt acaggccaag cgcctgcaga tttgacacct tctttagcca gtttcccgaa
     3241 atcatgcaag tgaatgttac atggtgtatt ttccgcccaa gtcgaaatca ctcccacaat
     3301 cgatgtttca aagtccttat ctgtcatacc agtcgcacga agcatagcac ggttaggtga
     3361 tttaaccatg ctgtcataga tgctactgcg gtgacgttta tctaattcag tcatcttatc
     3421 cctcccattt cagtttttac tattatagca caatttaaag gcaatgaaca gaataaaatt
     3481 cttgaatttt cagaaaattc tatacgcatg tgaaatattt aaaattaaaa acaacaaagc
     3541 ggattagtgc actttctgat gaccagaata tgctttttaa tccgctttct ttaaataacg
     3601 tactgtaatt tttacagaaa ttctttcaga taagtgtatt taacatctat cttgcattat
     3661 aaatttctag aaccttctct tttatattcg attcactcaa accatactca ttaagaagat
     3721 aatccatttt ccctacttga ccgaatcttt cttgaacacc catccgatga atttttgtta
     3781 ttccatcatc agagaataat tcacataaag cactgccaat tccacctatc tgattgtggt
     3841 tttctacagt aaatatagtt tttccactta acattgtttt tatctgttct ggtatcggtt
     3901 tgattctaaa taaatctatc acacttactg aataacctaa tttagacagt tcatctgcaa
     3961 ctcgaatact tggagcaacc attataccag aagcaacgat tacaagatct tcaccatgcc
     4021 ttaactcaat gtagccttta gaaaaatctt ctccaccttg atacacagga actggagctt
     4081 ttctaattgt tcgaatatat tttagtcctt ttaagtctaa tgtctggttc aatatttcac
     4141 gaaattggat atcatcagtt gcttcaaaaa tgatcgattt aggaattaaa cgtaacaatc
     4201 caatttcttc aaatggcata tgtgtcccac cattcatttc tgcagttact cctgcatctg
     4261 atccaatcac agtggcatcc aattgtgcgt atccaagaga aataaataat tgatcaaata
     4321 ctcttcgtga agcaaaagga ccaaatgtat gaagataagg tctaaacccc tgaatagaca
     4381 agcctgctgc aaggccgacc atttctgctt ccataatccc aacattcaca taacggtctc
     4441 caaagtcctt ttcaagatta tgagtagcca tcgaacttga caaatcggct tctaagacta
     4501 ctatatcaga atcactttga ttagcctcta aaaggaagtc tctatataca tgccgtaatt
     4561 ctttcgtgct tctcatcatt ctgtttcctc caattcctga cttaatcttt ctacaactga
     4621 ggttaacatt tgtctctcct ctacagtagg gcgaagatga tgattggatt tcatttcttc
     4681 cagctcttga accccttgac ctttaatagt atctaataca atgcacttag gtgatgaatt
     4741 atttgactgt tttaattgga caatcccttc ataaatttct ctaatatctg aacccttgac
     4801 cctaatggat tcaaatccaa atgctgaaaa tttttctacg aaatcacctg gattacaaat
     4861 atcctttgta aaaccatcta attgtttttt gttatcatca acaaatacaa ttaagttgga
     4921 taactgttga tgagaagcaa actgtatagc ctcccaacat tgtccctcat ttaactcacc
     4981 atctccaaca atagcataag tataaaaggg actctttctt attctctgac catatgcaag
     5041 tccagttgca acactaattc cttgtcctaa agagcccgtt gtcatatcta tgcctggcgt
     5101 tagatttcta tcaggatgag acggtaattt ggttccattt gtatttaaag aatataagaa
     5161 ttctttgtca aagaaaccat tcaaatagag tgtactgtat agagctggtc ctccatgacc
     5221 ttttgataat atgaaataat ctctatctcg tgctgcaaat atttctggag tcattggcat
     5281 tatttcacca taaagcactg ctaaaacttc tacgatagac agacttcctc cgtaatgtcc
     5341 gaatccaaga tgattcaatg ttctaagagt atttaatcgg atgttagtcg caaattttct
     5401 taacccatct tctctatttt tacttaaaat catcccttat tcctcctttg cagatggctt
     5461 tttaataaag gatactccaa acataactgc tagaataaga acaagaccaa tcacaatgcc
     5521 tgcttgtgag ccaaattgat ttaacattcc taaaataatt cctgatagac caaaatctgc
     5581 atctgagaaa gttgatcctt ggaaaccaag tcctcccaaa actggcatta aaaagactgg
     5641 aagaaaactg attaaaatac cttgtaaaaa tgctccaata gtggctccac gaacaccacc
     5701 agatgcattc ccaatgacac ctgcagtcgc tccacagaag aaatgaggca caacacctgg
     5761 taagataaca accgttcctg aagcaatcat aattgccata cttactaaac caccaataaa
     5821 actagagata aatccaatta gaactgcatt gggtgcataa gtataaacaa tcggacaatc
     5881 caaagcaggt tttgaattag gtacaagacg ctctgaaata cctttaaagg ctggaacaat
     5941 ttcgcccaaa ataaggcgaa cacctgctaa aataacaaat acccctgctg caaattgacc
     6001 tgctaattgt aaagcataaa ctagaccact tgtaccacta ctgatttctt tttctatata
     6061 ttctgaccct gcaaagatag ctacaataat gtaaataact gccatggata aagtaatact
     6121 aacagtacta tcacgtaaaa aagctaaact ctttggaaat ttaatgtcct ctgttgattt
     6181 tgatttgtca ccgataaggc taccagtaaa accactcaac caatatccca aagaactgaa
     6241 atgacctaaa gccaccttgt catttccagt taattgaacc atatattttt gaacaaatgc
     6301 tggggaaata ctcataataa taccgagtgc taatcctcct agtaagataa gaggcaagct
     6361 agtaaagcca gcaactgata aaatgaccgc aatcatacat gccatatata gagtgtggtg
     6421 ccctgttaaa aaaatatatt taaatcgagt aaaacgagcg attaagatat tgaacaccat
     6481 gcctgcaaac ataatcagtg cagtagctga gccatatgtt gttaaagcta cagctacaat
     6541 tgcttcatta ttcggcacaa cgccagataa atgaaaagca tgctcaaaca tggtaccaaa
     6601 tggatttaaa gaattttgta caattcctgc accaccagat acaactaaga aaccaacaaa
     6661 ggtcttaatt ccacctttaa taatatcagg taatttcttc ttctgaagaa ctaatcctaa
     6721 aattgcaatt aaagctacta aaatagctgg tgtactaaca atatccaata tgaacttcat
     6781 catgacgcta gcctcctata taagtccttt ttcttcacaa agtttagtaa ttaattctcg
     6841 tagttcatcc atatcaataa tactatttaa gatacgaaca tctccaagat gactagctga
     6901 atcagctaga tcacgaccaa caatccaaat atcagctgca tttggatctg ctccacctaa
     6961 atcataatgt tcaacttcta catctgaaac attcaaatca ctcaatacag attcaatatt
     7021 catctgtacc ataaaacttg aacctaatcc tgaaccacaa gctgtaccaa tttttaacat
     7081 tatctaatcc tcctgtttaa ttatcatttt aatgtcatca tagttttttg atgatattaa
     7141 agtttgaaca tgatttttat ctcttaaaat tgttgttaaa tgtgacaaag cctttaaatg
     7201 actctcatta tcaatggctg caatacaaat caacaatctt acctcttgtt ctggattatc
     7261 caataaataa atcggttctt ccaaaactaa cattgacatt cctatttcat tcacaccttc
     7321 atctggccga gcgtgaggaa ttgctactcc cttccctaaa ttaataaaag gcccaaactc
     7381 ttctactttt tgaatcattg cctcagggta gttctcagtt atcttatctt gatccaaaag
     7441 cggtttagct gctaaacgaa tcgcctcctt ccatcctaat ttttgcgaac taacctgata
     7501 ggtttctttg gtaatgagtt gttctagcac tggtacaatt tcctttctat catttttttg
     7561 gtaaagataa ttctttaacg ccaatcttaa ttccaattct tgtgtaataa ttccatatct
     7621 tttgacaata ttcagaattt gttcaatctc aaaatcccca tactctaaat tcggaaaatc
     7681 ttttaacact agttctacta gttgtattgc ttgctcttca gtcatcataa ccgaaactag
     7741 ataatttggc ttttctgtct ccacctttat ggtagaaaaa accatatcat agtcactact
     7801 agctttcacc tgtaaatcat caatctttga ggttcctata aactcaattt gaggaaataa
     7861 tgctaataga ttctctttta acatcaatga agaactaaca ccattaggac aaatgattgc
     7921 tgctttatac catttttgag gcaaagtatc tgctttcttt aaataacctc cgaaatggat
     7981 aacaaaatat gctgtttcac tatcaggtat gggattgtca atagcgtcca tcaagggcat
     8041 caaagaatct ttgactaatt caaataaatc aggataatat tctttaacat gcaatacata
     8101 ttcatttgaa ctaggtaggc cgaactttaa tctatagtaa gccggtataa ggtggcggcg
     8161 aagattttct ctcaatcctt ccctttgttt aaaatgtaac aaagaaatat cttccattct
     8221 acttataata gcctctgtta attgattaaa gtaaaccgga gcaacatcta cttcaccttc
     8281 aaagcaactt gataataaaa ctgtgatata gcgataatca tcctcagaaa acaccgtatc
     8341 tataattccc aaatcaacca ctgtatccaa taaaatagtg gttatatctt gaataacagg
     8401 agatactaat gtctctgaaa gacatactct ttcaacatcc ctttgatacc tacacagaat
     8461 gaatactaaa ccgaaaaggc aaacttttaa ttgattaaca ataggtacta gttgtagctt
     8521 ctcataataa tctttaacta cctgatttat caaatcataa gttaatgaat acccccaact
     8581 ggataaaaca taatccaaac cccaaatccc tatggaggat tccagcaact cactaaccat
     8641 ttgaaaagct aagcggtgct tattccactc tgaaccgtgt aaagtataac cttttgctct
     8701 actgtaccct agctccaaat cattatctaa cataatcttt cttaatgatt gaatatcaga
     8761 taaggttgta ttcttactta ctttcaaaaa gtcttggtaa tgattattcg atataaaatc
     8821 taatcggcaa aaagtgtaaa gatagattaa agctaagcga gtcgactttg gtaaaaccaa
     8881 ttcatccgac ttaataatat ctgtcaaaga ctgcttcgta cgatttgata aactatagcg
     8941 accttgtttt ttatccagca ctatcccttt attagctaga taaggcacta aataatctat
     9001 tccttctttg acttccttta taggtaagct caccttaaca gataattcat ataatgatag
     9061 ctcacaatga tccatcaaag tcatcaaaat aactagtgct ctataatcaa acatttctct
     9121 tcctttctaa ctacaattat agtgtaaaaa ttataagaat aaaagagtat ttgagcacaa
     9181 tataatctca ccttttaaaa gaaattattg ggctgaaagt ttttagtgat tattttcaac
     9241 tttctattga cttttaaaaa caaagtgata tcatttagat atcaaataat gggaggtcat
     9301 tcttatgact gaaaaactaa tcaattcaaa accaaatggt gtattcgcat tgattctcat
     9361 tgagttgaca atcgtacttg gtgtctttat atttataatg ggtgttggtt cggaaaacat
     9421 ttttggaatt attatcggac ctttactaat cgtaattgca ggtctagctc atgctggttt
     9481 aaaagttgtc aaacctcagg aagctctggt tctgacactc tttggtaact atacaggtac
     9541 catcaaagaa cctggttttt actttgtcaa tcccttcagc gtagcagtca accctgcaaa
     9601 ccacactcga cttggacaaa gtggtgatgt tagcacaaaa tctccttttt taggagctaa
     9661 atcatcaaat gacaatgatg taaatcttga aattggcaag aaacagattt ccctcaaagt
     9721 catgaccttg agcaattctc gtcaaaaaat caatgattgc ttaggaaacc ctgtagaaat
     9781 cggtatcgcg gtaacttgga gagttgtcga taccgctaag gcagtcttca acgttgataa
     9841 ctacaaagaa tatctttcat tgcaatgtga tagcgccctc cgtaatattg tccgcatcta
     9901 tccttacgat gtgtctccta atgtggatac tacgggtgac gggcaagcag atgaaggtag
     9961 tctccgtggc tctagcgaaa ttgttgctaa ccgtattcgt gaagaaatcc aaagtcgtgt
    10021 tgaggatgct ggcttggaaa tccttgaagc acgtatcact tacctagctt atgctccaga
    10081 aattgctgcc gttatgcttc aacgccaaca agcatctgcc attattgatg cacggaagat
    10141 gattgtagat ggtgctgtag gaatggttga aatggcacta gaacggctca atgaagggga
    10201 attggtagaa cttgacgaag aacgaaaagc tgccatggtt tcaaatctcc ttgtcgttct
    10261 ttgtggcaat catgatgcac aaccaattgt caacacagga agtctttact aaagatggct
    10321 gaagctaaaa aaaagcagat cccccttcga ctctcaacaa agttatacgc tgcactcgca
    10381 tcatgggcag aagacgactt tcgttcagtc aatgggcaaa tcgaatatct cctcacagag
    10441 tgtgtcaagc aacggaagaa agacggaaaa tacgtatcag aaaccattga cgaaccattt
    10501 gagattgata tttaacatct tctcctgtct gacaagtcgg acaggagaat tttttcatct
    10561 ttttttgtca agtaacttta ctttacaaaa aaaatgtgtt atcctagtat ggttgatgaa
    10621 aatcagtaga ttgaatcgaa tataaatacc taaaggagaa atcaaaatgg cagtacctgc
    10681 acgtcgcact tcaaaagcga agaaaaacaa acgtcgtaca cactacaaag taacagctcc
    10741 atctgtaaac tttgacgaaa ctactggaga ttactcacgt tctcaccgtg tatcacttaa
    10801 aggatactac aaaggacgta aaatcgctaa agctgcatca gctgaataat agaagggaga
    10861 taccatgcgc gtaaatatta cacttgaaca caaagaatct ggtgaacgct tgtaccttac
    10921 ttctaaaaac aaacgtaaca ctccagaccg ccttcaattg aagaaatact caccaaaact
    10981 tcgcaaacac gttgtgttta cagaagttaa gtaagcattc aatacgaatc aatacagaag
    11041 aaaaacgctt gatcagcgtt ttttcttttt gttaatttca ctatattgca agggaaatca
    11101 ttcgaatgac tacatttttc actacctttt ttgaaataag aatacggatt cggacgatta
    11161 aaagtaagag aaactcaaat gtaagttggg gttccttttt tgtatatttt tcaaataacg
    11221 cctctacact atttgtagca aattcaccaa ctacagttgt atcttagtta aaataagtta
    11281 gaatatgtaa gtgagtacca gatataccaa gacatcgtca ccatctaagg tatattcaaa
    11341 atacaaaagt tgaccaacta gatttctgaa tatccttata tatccattct taaaattggt
    11401 ttaaatagcg tagtctttta aactagtttt gagaatccaa aaaatcttcc tacatatgta
    11461 agaagatttt ttagttcaga atgattagat ttagctaatg gatacctatc ctacccactc
    11521 accgttagca ttgactcgat agccatcggg tgtagtcgtg tttactaata aatctcctga
    11581 attataggta tagtaccatt taccagaaac tgtaa
//
LOCUS       DBERYS010000054        11595 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.054, whole genome shotgun sequence.
ACCESSION   DBERYS010000054 DBERYS010000000
VERSION     DBERYS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.054"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="ACL8LA_001783"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="ACL8LA_001783"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS1813499.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="ACL8LA_001784"
     CDS             complement(1169..1693)
                     /locus_tag="ACL8LA_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGS1813500.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /gene="rpsT"
                     /locus_tag="ACL8LA_001785"
     CDS             complement(1746..1982)
                     /gene="rpsT"
                     /locus_tag="ACL8LA_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGS1813501.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(2050..2970)
                     /gene="coaA"
                     /locus_tag="ACL8LA_001786"
     CDS             complement(2050..2970)
                     /gene="coaA"
                     /locus_tag="ACL8LA_001786"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGS1813502.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3161..3564
                     /locus_tag="ACL8LA_001787"
                     /pseudo
     CDS             3161..3564
                     /locus_tag="ACL8LA_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3771..4361
                     /locus_tag="ACL8LA_001788"
     CDS             3771..4361
                     /locus_tag="ACL8LA_001788"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1813503.1"
                     /translation="MSKMYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDF
                     GSQLLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEK
                     NNAKATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4358..5635
                     /locus_tag="ACL8LA_001789"
     CDS             4358..5635
                     /locus_tag="ACL8LA_001789"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGS1813504.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5653..6315
                     /gene="deoC"
                     /locus_tag="ACL8LA_001790"
     CDS             5653..6315
                     /gene="deoC"
                     /locus_tag="ACL8LA_001790"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGS1813505.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6302..6691
                     /locus_tag="ACL8LA_001791"
     CDS             6302..6691
                     /locus_tag="ACL8LA_001791"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS1813506.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     ETLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="ACL8LA_001792"
     CDS             6782..7834
                     /locus_tag="ACL8LA_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGS1813507.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7978..9513
                     /locus_tag="ACL8LA_001793"
     CDS             7978..9513
                     /locus_tag="ACL8LA_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813508.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGAINADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="ACL8LA_001794"
     CDS             9506..10564
                     /locus_tag="ACL8LA_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813509.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="ACL8LA_001795"
     CDS             10567..11523
                     /locus_tag="ACL8LA_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813510.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3307 a         2077 c         2653 g         3558 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgtgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agttgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc atcttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ctactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttaattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctt ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatatag
     3061 tataagtgat caatttattg atgaaatagg agatagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttagtag gaggtgtact atgaaaatct taaaacgtta
     3181 tatattggaa ctctgtttta ttttaagttt tgctttacct tttataaaag gaaccaatgc
     3241 agataatggt agatgctttg tggaaaccta ttacggtttt acttttttga tggaacatgc
     3301 tattgtaaca gctgtcttta tctgttcgtt cttaattgct ttcttactaa aaaacgatgg
     3361 acgaaatgga ttgctgcggg tagttattgc tttttagtcc tacggattgc gacagagggt
     3421 tatttcttcc acatgtcact agaagatttg atacgacttt ggacaagttt ggaaatcctg
     3481 acacaaacgt atcagttggg cttttatcta aacattttgt taggaatttt gttgataata
     3541 aagtatttta aggttaagca atagaaataa taatctgcct gattatagat gataaacttt
     3601 ttgtttttat ctggactgtg atatttatag ttctaaatgg tttattattt ataagtgttg
     3661 aaagagattg aagaaaatgg cttcgtgact tgctttcttc ggtctttttc gttttattat
     3721 caatttgtaa acgatttaca atttgagtca aattatggta aaatagtgtc atgagtaaaa
     3781 tgtactatgc agaaaatcct gacgctgctc acgacattca tgagttgaga gtggacttgt
     3841 tgggagaaaa aatgactttt ttgacagatg cgggtgtttt tagcaagaaa atggttgact
     3901 ttggaagtca actcttgctc aagtgtctgg atgtcaacca aggagagaca gtcctagatg
     3961 taggctgtgg ttatggtcca ttaggtttgt ccttggtcaa ggtttacgga gttcaggcga
     4021 ccatggttga tattaacact cgtgccctgg atttagctca gagaaatgct gaaaaaaata
     4081 atgcaaaagc gacgatattt caatctaata tttatgcaag agttcaaggg cgatttgacc
     4141 atgtcatttc caatcctcct atccgtgcgg gcaagcaagt cgttcatgaa atcatcgaga
     4201 aaagtaaaga tttcttggaa attggtggag atttgaccat cgtcatccag aaaaaacaag
     4261 gagctccaag tgccaaatct aagatggaag atgtgtttgg caattgtgaa atccttaaaa
     4321 aagacaaggg atactatatc cttagaagtg tgaaagaatg agagcagttg atttaatcca
     4381 aaaaaaacga gacggtcaag aactgacttc aagtgaaatt gaatggctga tagaaggcta
     4441 tgtatcagga actgttcctg attatcagat gtctgccttt gctatggctg tttattttaa
     4501 aggaatgacg actcgagaaa tctctgattt aacgatgaat atggttaaga ctgggcaaga
     4561 gtttgacctg tcagccattg atggtgtgaa agttgacaag cattctactg gtggtgtcgg
     4621 cgataaggta accttgattt tagctcctct tgttgctagc ttcggtgtgc ctgtagcaaa
     4681 aatgagtggt cgtggtcttg gccatacagg cggaacaatt gataaattgg agtccattag
     4741 gggctatcaa gtagaacgta gtcaagagga ttttattcgt caggttcagg atattggagt
     4801 atctgtcatt gggcaatcag accagctggt caaagcagat aagcttctct atgcccttcg
     4861 tgatgtgacc gcaactgtcg acacgattcc tttgattgcg agttcggtga tgagcaagaa
     4921 aattgcggca ggggcggatg ctattttgct agacgtgact gtcggtgagg gtgccttcat
     4981 gaagacggtt gatgaggcgc gtgagttggc tcaaactatg gtagatcttg gtaaggttgt
     5041 tggacggaag acagtagcag tgattacgga tatgagccag cctttggggc gagcgattgg
     5101 aaatcgtctg gaaatccttg aagcattgga gattttgcaa ggtcaaggac gtcaggatat
     5161 tacccacttt atttgtgaat tggctcaaat tatgcttggt ttggcaaatg ttaacaaaac
     5221 tgttgaagaa gttcgccaac atcttgaaaa tggtcaagca ctggctaagt ttgaggaaat
     5281 ggtgcaagcc caaggtggag acttggaaga cctctatcgt cctgtaaatg tagctcatgt
     5341 ggtggaaatc cctgctcagg aaacgggtgt catttcagct cttccagcta tggactttgg
     5401 tctttatgcc atgaggctgg gtgctggtcg tgcagttaag tctgatgtct tggactatga
     5461 aacaggaatt gtttttgaaa agaagattgg agactccgtt caaaagggtg aaattgttgc
     5521 aaaagtatac acaaatgaaa aaattccacc tcaactagtt acagatttcc aaaaatgtgt
     5581 taaaataagt gatgaagtga aaaaaatccg tgaaattgta gaaatcatct cctaaatgga
     5641 gggaaaaccg aaatgaaatt aaataaatat atagatcata cgcttttaaa acaagatgca
     5701 aagaaaaaac aaattgatag tttgttgtct gaggctagag agtatggctt tgccagtgtt
     5761 tgcgttaatc cgacctgggt tgaacatgct aaaaaaggac ttgaaggcac agatgttaag
     5821 gtttgcacag tagtaggttt ccccttggga gcaacaactt caactgtgaa agcttttgaa
     5881 acaaaagaag ctatccaaaa tggggctgat gagattgaca tggtaatcaa tgttggggct
     5941 ctcaagtcag gtgatttggc cttggttgag tcggatattc gtgcagtagt ggaagcaagt
     6001 ggggacaagt tagtgaaagt tattattgaa gcttgccttc tgacagacca agaaaaagtt
     6061 ttggcttgcc aattagccca aaaagcagga gctgactttg tcaaaacatc tactggcttt
     6121 tcaactggtg gtgctacgat agcagatgtt agattaatgc gtgaaacagt tggacctgat
     6181 atgggtgtca aggccgccgg tggagctcgt tcttatgcag atgctcttac ctttgtcgaa
     6241 gcaggtgcga cccgtatcgg aacgtcagct ggggtagcta ttttaaaagg agaattggca
     6301 gatggcgact actgagttga ttgaactggc aattgaaacc agcaaacatg cctatgtccc
     6361 ctattctcac tttcctatcg gagcagtttt agtagccaaa gacggaagtg tttacacggg
     6421 agtgaatatc gaaaatgcta gctatccttt gaccaattgc ggtgagagaa cagctatttt
     6481 taaggctata tctgaaggcc aaagagaatt ttcagaattg attgtctatg gtcaaactga
     6541 aaaaccgatt tcaccatgtg gtgcttgtcg ccaagtaatg gtcgaatttt ttgaacaaga
     6601 tttaaaagag accctagtcg caaaagataa atcgacggtc gagatgacgg tcggggagtt
     6661 acttccatac tcttttacag acttaaatta gtctgagtcg ctctttgagt ggcaagggtc
     6721 tttgtgacca atcaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcaa gactggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttt ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaaa atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 ggcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtac aagttgacta
     7381 cgctggttca tttggtgatg cggctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgaccg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag ttcctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaagcttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aaccagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagca
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta tcggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctatcaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtgttgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg agattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaaggac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta ttcctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gccctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttagct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactc tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agctcgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctttataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccttggg aatcaacgtc
    11161 tacaagatga gatatttagg ggttattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactgcc ccccaaaagt tagacagaaa
    11581 aaatctaact tttgg
//
LOCUS       DBERYS010000055        11149 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.055, whole genome shotgun sequence.
ACCESSION   DBERYS010000055 DBERYS010000000
VERSION     DBERYS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.055"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(68..1048)
                     /gene="dusB"
                     /locus_tag="ACL8LA_001796"
     CDS             complement(68..1048)
                     /gene="dusB"
                     /locus_tag="ACL8LA_001796"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGS1813511.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1035..1907)
                     /gene="hslO"
                     /locus_tag="ACL8LA_001797"
     CDS             complement(1035..1907)
                     /gene="hslO"
                     /locus_tag="ACL8LA_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGS1813512.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2072..3499
                     /locus_tag="ACL8LA_001798"
     CDS             2072..3499
                     /locus_tag="ACL8LA_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1813513.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            3658..5166
                     /gene="glpK"
                     /locus_tag="ACL8LA_001799"
     CDS             3658..5166
                     /gene="glpK"
                     /locus_tag="ACL8LA_001799"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGS1813514.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNFVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5208..7034
                     /gene="glpO"
                     /locus_tag="ACL8LA_001800"
     CDS             5208..7034
                     /gene="glpO"
                     /locus_tag="ACL8LA_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGS1813515.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7104..7808
                     /locus_tag="ACL8LA_001801"
     CDS             7104..7808
                     /locus_tag="ACL8LA_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS1813516.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8105..8308
                     /locus_tag="ACL8LA_001802"
     CDS             8105..8308
                     /locus_tag="ACL8LA_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813517.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8395..9003)
                     /locus_tag="ACL8LA_001803"
     CDS             complement(8395..9003)
                     /locus_tag="ACL8LA_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813518.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            9135..9326
                     /locus_tag="ACL8LA_001804"
     CDS             9135..9326
                     /locus_tag="ACL8LA_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813519.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKNK"
     gene            10137..>11149
                     /gene="ltrA"
                     /locus_tag="ACL8LA_001805"
     CDS             10137..>11149
                     /gene="ltrA"
                     /locus_tag="ACL8LA_001805"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGS1813520.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVVSEAAAKRMMYSVSRFIEKRLGLKANMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVA"
BASE COUNT         3284 a         2175 c         2558 g         3132 t
ORIGIN      
        1 tttttttgat gaggaaagaa tggcggcact caagagactc tttaagagag ttacgggttt
       61 taaactatta agccttctcc aattgcaaga gagcttcaat ctctgccagg gtgctagctt
      121 gcgaaatggc tccacggagt ttggcagcgc cagatgttcc acggaggtag tgaggagcga
      181 ggccacggaa ttcacgaact gcgacgtttt ctcctttgag gttaatcaat cgtttcaagt
      241 gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc aggtaggatt tctcctgttt
      301 caaagtaatg gttgatttgg ttgaagaggt aaggatttcc catggcagct cggccaatca
      361 tgactgcgtc agcaccaact tcttcgatgc gttgcttggc ttcttggaca gtacggatat
      421 caccgttggc gatgaatgga atcttggtta gagcttgggc aaccttgtac agggtctcaa
      481 ggtctgcgtg gccagtatac atttgttcac gggtacggcc atgcatggcg agggcagaaa
      541 cacctgcagc ctcagcagcg agggcatttt ctactgccag agatgggtcc gcccagccgg
      601 tacgcatttt gacagtaagt gggatatcaa ggacagactg gaccttgttg atgatagagt
      661 agatcttgtc aggatccttg agccacatag ctccagcttc gttcttcacg attttgttga
      721 cagggcagcc catgttgata tcgacgatat cggtcttggt gttttcttgg atgaattctg
      781 ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg gatagagaca gggttttcgc
      841 cctcatcgat atgaagcatg tgcagggttt tttcgttgtt gtattggatt cccttgtcag
      901 agaccatttc cattacaacg agtccagctc cgagctcttt tgcgatggta cgaaaggctg
      961 agttggtcac gccagccata ggcgctaaaa cggtacgatt gggaatctca acattgccaa
     1021 tcataaaagg tgtattaaga tttgtcacga atgagttcct ccaggtcctt ttcatcaaag
     1081 ttgtaagtag tttggcagaa ttgacaagtg atttctaccc cgtggtcttc ctctttcatt
     1141 tcctgtaagt ctgagcttgg aaggctggca agagcgttca taaagcgttc atggctacag
     1201 tcacattgga aacggatttc ttcttcagaa agacgcttgt aggcttcgtc cccgtagata
     1261 gccttgagga gggcttcgat atggtcgtcg ctttcgagaa gagtagagat agctggcatt
     1321 tcttggatgc gtttttcaaa gcgagcaatc tcttctttct tggctcctgg caagacttga
     1381 actaggaaac cacctgcaac cttgaccttg tcttcctcgt ccaaaaggac attgaggccg
     1441 accgctgaag gcgtttgttg gctttcagta aggtaaaagg caaggtcttc accgatttct
     1501 ccagagatga ggggagttat agagttgtaa ggatttccag caccgtagtc tgtgataacg
     1561 aggaattgac catttccaac aaaaggtccg actaggactt caccagtcgc agtctttttg
     1621 atgtcaacac caggattttg aacatagcct ttgacgttcc ccttggtatc agcgacggtg
     1681 ataatagcac ctagagagct agagcccaac accttaactg taagtttggt atttcctttt
     1741 tcattggctg cgagaatctg gctggcgata agagttcgac caagcgctac agttgagcta
     1801 gcttgggttt gatgtttttc ttgagcagtg cggacggttt ccgtgctatc aaggacaaaa
     1861 gcacgaaagg ctccgctttc tgatatagtt ttaataattt tatccatagc tactatttta
     1921 gcataaaaat gcccaaaggg ggagccgtgt gtttactgat tttcaggata atggaccagg
     1981 aaatcagcat gaaaataaaa agagaaacag attattttag catttgtaag atttatgcta
     2041 tgcttaaggt agaaaatgaa aggggtaaca aatgtattta ggagatttga tggagaaagc
     2101 cgagtgtggt caattttcaa tcctttcctt tctattacaa gagtctcaga cgaccgtcaa
     2161 ggctgtaatg gaagaaacag gattttcaaa agcaacccta accaaatatg tcaccctgct
     2221 caatgacaag gctttggata gtggcttaga gctggctatt cactcagaag atgaaaatct
     2281 gcgtctgtct atcggtgcag ctaccaaggg gagagatatt cggagcttgt ttttggagag
     2341 tgctgttaaa taccagattt tggtttatct tctctaccac caacagtttt tagcccatca
     2401 gctggctcaa gaattggtga ttagcgaggc tacgcttggt cgtcacttgg ctggtttaaa
     2461 tcagattttg tcagaatttg atttatccat ccaaaatgga cgttggcgag gtccagagca
     2521 tcagattcgc tatttctatt tctgtctttt ccgaaaggtc tggtcgagtc aggaatggga
     2581 aggtcacatg cagaaaccag agagaaaaca ggagattgcc aatttagagg aaatctgcgg
     2641 tgcaagtttg tctgtggggc agaaattgga cttggttctc tgggctcaca tcagtcaaca
     2701 acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa gagaaaatgc gagggtattt
     2761 tgacaatatt ttctaccttc gtttgctgag aaaggttccg tccttttttg ctgggcaaca
     2821 tattccacta ggagttgagg atggtgagat gatgatattc ttctcttttc tcctatctca
     2881 tcgcattctt cctcttcata ctatggagta tattcttggt tttggagggc agttggcaga
     2941 tttactgacg caattgattc aagaaatgaa gaaggaggaa ctattggggg attatacaga
     3001 ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa gtctatctct ataagggcta
     3061 tattttacag gatcgctaca agtaccagtt agagaatcgt catccatatt tactgatgga
     3121 acatgatttt aaagagacag cagaggagat ttttcatgct ctacctgctt ttcaacaggg
     3181 gacagattta gataagaaga ttctctggga atggctccag ttaatcgaat atatggctga
     3241 aaacggtggc cagcatatgc ggattggtct ggatttgaca tctggttttc ttgtcttttc
     3301 aaggatggca gccattttga aacggtattt ggaatacaat cgttttatta ccattgaagc
     3361 ctatgaccct agtcggcatt atgatttgct ggttaccaat aacccgattc ataagaagga
     3421 acagacacca gtctattatt taaaaaatga cttggatatg gaggatttgg tagcgattcg
     3481 ccagttatta ttcacttaaa aggcttggtt aatccaggtc ttttttgtga aattcacaca
     3541 atctcctcac atttttttaa aaattaaaaa aagttgataa acaagaaagc gctttatttt
     3601 gtatactagt aagtgtaaag aggaaacacc tcaagatctt tatcaggagg acagtacatg
     3661 tcacaagaaa aatacatcat ggccattgac cagggaacta caagttctcg tgccatcatt
     3721 ttcaacaaaa aaggggaaaa ggttagctcg agtcaaaaag agtttaccca gattttccct
     3781 caggcaggtt gggttgagca caatgccaat gaaatttgga actttgttca gtcagttatt
     3841 gcgggtgctt tcatcgaaag tggtgtcaag ccaaatcaaa tcgaggcaat cgggattacc
     3901 aaccaacgtg aaacaacggt tgtctgggat aagaaaacag gacttcctat ctacaatgct
     3961 atcgtttggc agtcacgcca gacagcacct ttggctgagc aactaaaaag ccaaggttat
     4021 gtggaaaaat tccatgaaaa gactggtttg attattgatg cttacttctc tgctaccaag
     4081 gttcgttgga ttttggatca tgtagaaggt gctcaagagc gagcagaaaa aggggaattg
     4141 ctctttggta ctatcgatac ttggttggtt tggaaattga ctgacggtgc ggctcacgtg
     4201 actgactact caaatgcagc tcgtaccatg ctttataaca ttaaagaact caaatgggat
     4261 gatgagattt tggaaatcct taacattccg aaggctatac ttccagaagt tcgttctaac
     4321 tccgaaatct acggcaagac agctccattc catttctacg gtggagaggt gccaatctca
     4381 ggtatggctg gggaccaaca agcagccctc tttggacagt tggcttttga gccaggtatg
     4441 gttaagaata cttatggaac aggctctttc atcatcatga atactgggga agagatgcag
     4501 ttgtctgaaa acaacctctt gacaaccatt ggttacggaa tcaacggtaa ggtttattat
     4561 gccttggaag gttctatctt catcgcagga agtgctattc agtggcttcg tgacggtctt
     4621 cgcatggttg aaaattcacc agaatctgaa aaatacgctc gtgattctca caacaacgat
     4681 gaagtttatg tcgttccagc ctttacaggt ctaggcgctc catactggaa ccaaaatgct
     4741 cgtggttccg tctttggttt gactcgtgga acaagcaaag aagactttat caaggcgact
     4801 ttgcaatcta ttgcttatca agtgcgtgat atcatcgaca ccatgcaagt ggatactcag
     4861 accgccattc aagtactgaa ggtggatggt ggtgcagcca tgaacaactt cctcatgcag
     4921 ttccaggcgg atattttagg cattgacatt gcacgtgcta aaaacttgga aacaacagct
     4981 ctaggagcgg ccttcctagc aggtttgtca gtagggtact ggaaagactt ggacgagttg
     5041 aaactcttga acgagacagg agaactcttt gagccatcta tgaacgaatc tcgcaaggaa
     5101 caactctaca agggctggaa gaaggctgtg aaagcaactc aagtctttgc ggaagtagac
     5161 gactaatact ggcagaataa agcgatttat ttagaaagtg tgtaaatatg gaattttcaa
     5221 aaaaaacacg tgaattgtca attaaaaaaa tgcaggaacg caccctggac ctcttgatta
     5281 tcggtggagg aatcacagga gctggtgtag ccttgcaggc ggcagctagc ggtcttgaga
     5341 ctggtttgat tgaaatgcaa gactttgcag aaggaacatc tagtcgttca acaaaattgg
     5401 ttcacggagg tcttcgctac ctcaaacaat tcgacgtaga agtggtctca gatacggttt
     5461 ctgaacgtgc agtggttcaa caaatcgctc cacacattcc aaaaccagat ccaatgctct
     5521 taccagttta cgatgaagat ggagcaacct ttagcctctt ccgtcttaaa gtagccatgg
     5581 acttgtacga cctcttggca ggtgttagca acacaccaac tgcgaacaag gttttgagca
     5641 agaaagaagt cttggaacgc cagccaaact tgaagaagga aggcttggta ggaggtggag
     5701 tgtatcttga cttccgtaac aacgatgcgc gtctcgtgat tgaaaacatc aaacgtgcca
     5761 accaagacgg tgccctcatt gccaaccacg tgaaggcaga aggcttcctc tttgacgaaa
     5821 gtggcaagat tacaggtgtt gtagctcgtg atctcttgac agatcaagta tttgaaatca
     5881 aggcccgtct ggttattaat acaacaggtc cttggagtga taaagtacgt aatttgtcta
     5941 ataagggaac gcaattctca caaatgcgcc caactaaggg agttcacttg gtagtggatt
     6001 caagcaaaat caaggtttca cagccagttt acttcgacac aggtttgggt gacggtcgta
     6061 tggtctttgt tctcccacgt gaaaacaaga cttactttgg tacaactgat acagactaca
     6121 caggtgattt ggagcatcca aaagtaactc aagaagatgt agattatcta cttggcattg
     6181 tcaacaaccg ctttccagaa tccaacatca ccattgatga tatcgaaagc agctgggcag
     6241 gtcttcgtcc attgattgca gggaacagtg cctctgacta taatggtgga aataacggta
     6301 ccatcagtga tgaaagcttt gacaacttga ttgcgactgt tgaatcttat ctctccaaag
     6361 aaaaaacacg tgaagatgtt gagtctgctg tcagcaagct tgaaagtagc acatctgaga
     6421 aatatttgga tccatctgca gtttctcgtg ggtctagctt ggaccgtgat gacaatggtc
     6481 ttttgactct tgctggcggt aaaatcacag actaccgtaa gatggctgaa ggagctatgg
     6541 agcgcgtggt tgacatcctc aaagcagaat ttgaccgtag ctttaaattg atcaattcta
     6601 aaacttaccc tgtttcaggt ggagaattga acccagcaaa tgtggattca gaaatcgaag
     6661 cctttgcgca acttggagta tcacgtggtt tggatagcaa ggaagctcac tatctggcaa
     6721 atctttacgg ttcaaatgca ccgaaggtct ttgcacttgc tcacagcttg gaacaagcgc
     6781 caggactcag cttggcagat accttgtccc ttcactatgc aatgcgcaac gagttggctc
     6841 ttagcccagt tgacttcctt cttcgtcgta ccaaccatat gctctttatg cgtgatagct
     6901 tggatagcat cgttgagcca gttttggatg aaatgggacg attctatgac tggacagaag
     6961 aagaaaaagc aacttaccgt gctgatgtcg aagcagctct cgctaacaac gatttagcag
     7021 aattaaaaaa ttaagaaaaa ataaaagagg tggagggcag cattccttgt cgcccgtccc
     7081 ttctttttaa tggagacaga aagatgatga atgaattatt tggagaattt ctagggactt
     7141 taatcctgat tcttctagga aatggtgttg ttgcaggtgt ggttcttcct aaaaccaaga
     7201 gcaatagctc aggttggatt gtgattacta tgggttgggg gattgcagtt gcggttgcag
     7261 tctttgtatc tggcaagctc agtccagctc atttaaatcc agctgtgaca gttggtgtag
     7321 ctttaaaagg tgacttgcct tgggcttccg ttttgcctta tatcttagcc cagttcgcag
     7381 gggccatgtt gggtcagatt ttggtttggt tgcaattcaa acctcactat gaggcagaag
     7441 aaaatgcagg caatatcctg gcaaccttca gtactggacc agccatcaag gatactgtat
     7501 caaacttgat tagcgaaatc cttggaacct ttgttttggt gttgacaatc tttgctttgg
     7561 gtctttacga ttttcaggca ggtatcggaa cctttgcagt gggaactttg attgtcggta
     7621 tcggtctatc actaggtggg acaacaggtt atgccttgaa cccagctcgt gaccttggac
     7681 ctcgtatcat gcacagcatc ttgccaattc caaacaaggg agacggagac tggtcttacg
     7741 cttggattcc tgttgtaggc cctgttatcg gagcagcctt ggcagtgctt gtattctcac
     7801 ttttctagtt tatactcttc gaaaatcaaa ttcaaaccac gtcagcgtcg ccttaccgta
     7861 ctcaagtaca gcttgcggct agcttcctag tttgctcttt gattttcatt gagtattaga
     7921 aaacaattat gttgatagag cttgggcaag agcccaattt cagcaaaaaa tgaagtaaat
     7981 cttctcataa taaaacgcat catatcaagc acgaaaattc cacgaggtca actacagtca
     8041 gaaagctgaa caacaagcca aaacgcccaa aaaaggcggc aaaaagcaag cacctgcaag
     8101 caacgtgccg aaatggtcaa atcctgatta tgtcaacgaa ttagacccaa aaatcgttga
     8161 tatgctagta gaatttcaca agtcacaagg cactttggaa actcccgagg cgcaagcaga
     8221 aatcgcccaa aaacgtgaag aaatcgagca aaggagagct gagcttgagg ataaaaaaca
     8281 agagcttttg aaccgcttga acaaatagag tttcgcaagt attatgctta caaattactt
     8341 gagcaattaa ctaaaatata aaccctgcct agatataaag gcagggttta tattttagaa
     8401 attcacgtag gttgttacgg tttttacata cccagtatag tttgagtttc tatagtattc
     8461 agtgataaac ttccattttc tttgagcaac atggatataa gtacttgtta tgtagtatgg
     8521 atatgggctt tgtgaatcca agtaagactg ataagcttgt ataccaaaat atgctccacc
     8581 aattattgca ccccatggac cccccaataa agcacctatc ctaccaatca tataactgat
     8641 tccagcacca gtcatgaagt tagcgaatgt gttagcttgt ttattcccat gtattgtgtt
     8701 gacgtaattc caaacattag gatcgtatga tctaaaagat atatttaggt cgatttcatt
     8761 cttttgataa gccatataaa atgccccatt gatatagacg ccgtcagcac gtcgttcaat
     8821 agtgtctaca cttccatctg gattgacaac ctcaagaact tcatcgctta aaatatttac
     8881 ttgcgtatct ccgaaccgca ctgatgagcc attctcaaac tgagcctcac cagatacaac
     8941 tttagagttt gccgataagc tatcatcagc aaaaacaaca agcgacgggg aaatgctaga
     9001 catacagaaa acagacataa ctagcaaaca catgcattta aacatcttag acataacgga
     9061 aactcttttg tatttttgat ttttttcaac ttttattata caataaaacc aaataaaaag
     9121 aaagcggtaa caatatgctt aatgcgaaaa ttttttatat atttttatgt ttgatcgtta
     9181 tcgaaactac aggcttgttg ttgttgaaaa gaggtctcga aatgggttat ttagacacag
     9241 aagctattat cctcgcagtt ttttcatttg ctttttacaa cctatgttca ttcgcttggg
     9301 tctgctctac aataaaaaat aaatagacgt attttcaaaa aaaaaaaaat gaatatttat
     9361 attagcaaaa tgacgattta aatcgtcgtt tttttgtagt acgacgggca tgtcgtatat
     9421 ctgaggtgta agtcctcggt gggcacctgc taccggtgaa cccaatagcg attcccaagc
     9481 ctgactatcg tgaggtagcg gattaaaacg gtctggggat agaccgtttt aagtctgacg
     9541 caggaaataa gaatcgtcag aggaagggat agcgaaatcg tggctctacg aacaggaacg
     9601 tgatagtaag gcgtatatag cggataagga ggcttcaaac tctaaagtcc aaaaaggtag
     9661 tcgtaaccta tatgtgtaaa tcacgagagt aattgaattc ggactaaggt ttgtgtgaaa
     9721 aagataaatc tttctagagt ctaaagactc tgcgtcagat ttcctatttt cactgtaacc
     9781 ttttaacgtc ctcatatctt gtataaacga ggaaagatgt acgacttatc ccgtgaggtt
     9841 tcatgagcgc tgaaagcgta gtaacaacga atcatgagaa gtcagccgag cccatagtag
     9901 tgaggaaact tctgtagtgg aagtggagcg aaggggtgaa tactcaaaca gtctggggag
     9961 agactgtttg aggtctgtcg ctagaaagag aaaacgacag atcgaagtaa tcctacttca
    10021 cttgtgtctg taaaatgagt ggtctgatag aactggactt tgacacgtaa tgactagatg
    10081 aaggttcacc aatataagat gtccctcagg caccaaaaca agaaaggaat acgcacatgt
    10141 caaaactgct agataagata ttatcacgcg aaaatatgct ggaagcctac aatcaagtaa
    10201 aatccaataa aggctcagct gggattgatg gaatgactat cgaagagatg gataattatc
    10261 tcagacaaaa ctggcgcttg actaaggaac tgataaaaca gagaaaatat aagcctcaac
    10321 cagttcttaa agttgagata cctaaaccag acggaggcat ccgtcaacta ggaatctcaa
    10381 cagttatgga tagaatgatt caacaggcca ttgtccaagt catgagcccc atttgtgaac
    10441 cccatttctc agatacgagt tatggtttca gaccaaatag gtcatgtgaa aaagccatca
    10501 tgaagctctt agaatactta aatgacggct atgagtggat agtggatata gacctagaga
    10561 aatttttcga tacagttcct caagatagat tgatgtcctt agtacataac attatcgaag
    10621 acggagatac ggaatccttg attcgtaagt atcttcattc aggtgttatc attaatggtc
    10681 aacgttataa aacactagtt ggtacaccac agggaggaaa tttatctcct ctcttatcca
    10741 atatcatgct taatgaattg gacaaggaat tagaaaagag gggacttcga tttgtgcgct
    10801 acgcagatga ttgtgtgatt acggtcgtaa gcgaggcagc cgctaagcgt atgatgtatt
    10861 cagtcagtcg ttttattgaa aaacggctag gtttgaaagc aaacatgacc aagactaaga
    10921 ttaccagacc aagagagttg aaatatctag gttttgggtt ctggaaatca tcagatggtt
    10981 ggaaaagtcg tccacatcaa gatagtgttc ggagatttaa gcttaaattg aagaaactaa
    11041 cacagaggaa atggagtata gacctaacaa gacgtattga gcaactgaat ttgtctattc
    11101 gaggatggat aaactatttc tcattgggaa atatgaaaag tatagtcgc
//
LOCUS       DBERYS010000056        10884 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.056, whole genome shotgun sequence.
ACCESSION   DBERYS010000056 DBERYS010000000
VERSION     DBERYS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.056"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..864
                     /locus_tag="ACL8LA_001806"
                     /pseudo
     CDS             1..864
                     /locus_tag="ACL8LA_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(992..2014)
                     /gene="cbpF"
                     /locus_tag="ACL8LA_001807"
     CDS             complement(992..2014)
                     /gene="cbpF"
                     /locus_tag="ACL8LA_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HGS1813521.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRQEIAFRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWV
                     YDDGDWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQHLGNKWYYLRSSGAMATGWYQDGSTWYYLDAENGDMKTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2033..2854)
                     /gene="cbpG"
                     /locus_tag="ACL8LA_001808"
     CDS             complement(2033..2854)
                     /gene="cbpG"
                     /locus_tag="ACL8LA_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HGS1813522.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHSYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2935..3174)
                     /locus_tag="ACL8LA_001809"
     CDS             complement(2935..3174)
                     /locus_tag="ACL8LA_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813523.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3324..4082)
                     /locus_tag="ACL8LA_001810"
                     /pseudo
     CDS             complement(3324..4082)
                     /locus_tag="ACL8LA_001810"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(4083..4715)
                     /locus_tag="ACL8LA_001811"
     CDS             complement(4083..4715)
                     /locus_tag="ACL8LA_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1813524.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4729..5724)
                     /locus_tag="ACL8LA_001812"
     CDS             complement(4729..5724)
                     /locus_tag="ACL8LA_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1813525.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5721..6419)
                     /gene="liaF"
                     /locus_tag="ACL8LA_001813"
     CDS             complement(5721..6419)
                     /gene="liaF"
                     /locus_tag="ACL8LA_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGS1813526.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6496..7506)
                     /gene="fni"
                     /locus_tag="ACL8LA_001814"
     CDS             complement(6496..7506)
                     /gene="fni"
                     /locus_tag="ACL8LA_001814"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGS1813527.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7490..8497)
                     /locus_tag="ACL8LA_001815"
     CDS             complement(7490..8497)
                     /locus_tag="ACL8LA_001815"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGS1813528.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDILIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSFEEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8484..9437)
                     /gene="mvaD"
                     /locus_tag="ACL8LA_001816"
     CDS             complement(8484..9437)
                     /gene="mvaD"
                     /locus_tag="ACL8LA_001816"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGS1813529.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEYLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(9419..10297)
                     /gene="mvk"
                     /locus_tag="ACL8LA_001817"
     CDS             complement(9419..10297)
                     /gene="mvk"
                     /locus_tag="ACL8LA_001817"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGS1813530.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITETCVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10419..10643)
                     /locus_tag="ACL8LA_001818"
                     /pseudo
     CDS             complement(10419..10643)
                     /locus_tag="ACL8LA_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3224 a         2525 c         1922 g         3213 t
ORIGIN      
        1 aaagaagaaa gacagaaatt gttcaagaat taatgactga attttcgtta gctcttcttc
       61 taaaagtcat taaactagct cgttcgacct actactatca cttgaaacag ctagataaac
      121 cagataagga ctaagagctt aaagctgaaa ttcaatccat ttttatcgaa cacaagggaa
      181 attatactta tcgccgggtt catttagaac taagaaatcg tggttatctg gtaaatcata
      241 aaagaattca aggcttgatg aaagtactca atttacaagc taaaacgcga cagaaacgaa
      301 aatattcttc tcataaaaga cgttggcaag aaggcagaga atctcattca acgccaattt
      361 gaaggctcta aaacaatgga aaagtgctac acagatgtga cagaatttgc cattccagca
      421 agtactcaaa agctttactt atcaccagtt ttagatggct ttaatagcga aattatcgcc
      481 tataatcttt caacttcacc caacttagaa caagtacaaa caatgttgga acaggcattc
      541 acagagaagc actacaagaa tacgattctc catagtgacc aaggctggca ataccaacac
      601 gattcttatc atcggttcct agagagtaag ggaattcaag catccatgtc acgtaaggga
      661 aacagcccag acaatagtat gatggagtcc ttctttggga ttctgaaatc ggaaatgttt
      721 tacggttatg agaagtcgtt tcagtcgctt aagcaattgg aacaagccat tatagactat
      781 attgattact acaacaataa acgaattaag gtaaaactaa aaggacttag ccctgtgcaa
      841 tacagaacta aatccttcgg ataaattaat tgtctaactg tttgagggca gtacagaaaa
      901 accttgatac tatgcgtttt ttgattgtga agacttttat ccacctatta tacaaagtta
      961 agtatccatc acagtttaca attagccttc attacttaac ccattcacca ttatagttta
     1021 agtagtaacc acctactgtg gtattaacag ctaaagcacc tgaatcatag gcatagtacc
     1081 agttaccatt gacttggaac cagcctgttt tcatatctcc attacttgca tttagatagt
     1141 accaagtcga accttcctga taccaaccag ttgccatagc tcctgatgaa cggagatagt
     1201 accatttgtt cccaaggttt tgccaaccag ttttcatatc gccattttca gcatctaaat
     1261 agtaccaagt tgaaccatct tgataccagc cagttgccat agctcctgat gaacggagat
     1321 agtaccattt attaccaaga tgttgccaac ctgtttgcat gatgccagtt gctggatcta
     1381 gatagtacca tggagctgct tttagcttct cttcatcata ggtaagaggg taatccttaa
     1441 cccaaccacg tgctagctct ccaaccgtca atctgttgat gcgagaatca aagccaccat
     1501 ccttctgcaa ataataccag tccccatcat cataaaccca accagttttt aaagtatgat
     1561 aactacgctg atcttcaaaa taatagactc gtttctctgc tgggctatca tattcttccc
     1621 catgatgttt gttggtattt gtagcagttt ttgcttctaa cacttgcttg ccaacaaatt
     1681 cttgtagtac cccatcttga ccaaagtaaa accaatctgg tctaaaagca atctcttgtc
     1741 ttggagaagg accaatcgta acccccttgt gtggagcagg tatatattgc cagccgacaa
     1801 ccatctctcc ggataaagga tcaaaataat agtatttccc atctatcact ctccagtagg
     1861 tttcttttag atcccccttt ttgtagtagg ttctaccatt ttcttggaca aactgccatc
     1921 cttcagaatc atctgcaaat actgtactgg tccctagcaa accgaagaaa aatgtggcta
     1981 gtgcgacttg catcattttt ttcaaaagct tcattttata tatcctccaa cattaaatcc
     2041 actcaccaga tgaagcgaag ttataaactt taccatcgat agtttggcta cctgtaacca
     2101 ttgctccatt tgaattgaga taataccatt ttccatttac tttttgccaa tctgtaagca
     2161 tcttaccgga actgtctaaa taataccaag tatcatttat ctcctgccaa cccgtttgtt
     2221 tatcatgttg tctataatgg taccaactac catttatttt cttccatcct tcaagagagt
     2281 aacctttaag aaccgaataa ataaatggca aatttcgttc atttaattta actgcactgt
     2341 taatttgatt agctccatct cctaaagtat gcactcctac tacacggtga ctagcatcat
     2401 aaactgtaga tccactagac ccctctaaag tatcaacttg gtaatccaag aacatgccat
     2461 catcacttaa aacttgtttt ttatctgtat acatttgatg aactttaaaa ttatatgatg
     2521 gatagcctgt gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca
     2581 aagtccctaa ttttgcacca atgggctctt ctagaattaa taaagccaag tcatattccc
     2641 ttgcatcctt agaatttaaa tttctaaatt ccttcaaata acgaacttcc tttactttga
     2701 tctttccaaa tggttcttga cttggactaa cagccggaag aacataaata tcatccgctt
     2761 ctttcccata gtcatgtctg tagtagctgt gagccgctgt aattaacaca ttatctttaa
     2821 taaaattcgc acttcccctc ccttctacat tcattccatc ggctactcca taatacttag
     2881 ataaaaccat agctgaagtc gaatacggat actgtaaagt attatcaatt ttaatcaaat
     2941 catcattacc gataatactt ctgattgctt ttggtagtat gaaccatatg ttggtgaaat
     3001 ctcagataat gaagaatcat tagatcctgg acctttttct agtgtctcac ttacctcata
     3061 ttcttcaccc gtactagaaa taacactcaa agcagatact gtcgataact ggctagccaa
     3121 taaagtactc gcaataattg aaatacccaa ttttttataa acagttttct tcattattgt
     3181 atcctcctaa tgtaattata gcgtactatt ctaaatttct taatctacta tagaatcaag
     3241 aaatctacca ccttctttaa ataccctcca ttatcacata aacaggtaaa cttttcaatt
     3301 aatgactgcg cttttcaatc acgctagagg tacttacttg cttctttgat actaagttca
     3361 gccattcttt ccttgttttt ctcaataaag catgttaccc aagtgggatt cgttttggag
     3421 tagtctcgca gagtccagcc aatggcttta ttgataaaaa attctgtttg gttcaagtta
     3481 tgaaggagaa tcttttccat taattgagta ttggtcttct cttttcttaa caactggttg
     3541 tcaatagcga cacgtctcag ccagatatta tctgataggc tccattttat actcaatgaa
     3601 aatcaaagag caaactagga agctagccgc agattgctca aaacactgtt ttgaggttgc
     3661 agatagagct gacgtggttt gaagagattt tcgaagaata ttaagattat ttcttctagt
     3721 tcaggatgtt tatacaccaa actccctact actcgatcta ggatatctac cgtgtcccac
     3781 caggattttg tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac
     3841 tgcattgctt tcaaatagtt agcagccaca tattggtatt ttctaggctc cttttcccag
     3901 caagtgtctg caaaatccca atcgataatc tttgtttttt tcgcttctgg aaaatatttt
     3961 tatagagttt atttctttca acctagaaaa gaaaattgat ggcacatata ggcttccatg
     4021 ggccttgctt ttttagagtc ttttgctgct tctagctcct caagtaaatc tgctaaactc
     4081 atctaaaact cctcatgccc caccaaatgg tgctgaaagg catagacagc cgcctgggta
     4141 cgatcgctaa cttcaagttt ggcaagaatg ttggacacgt gggtcttgac cgtcttgaga
     4201 gagataaaga ggtcatctgc gatgcgctga ttttcgtagc ccttggcgat gagttggaga
     4261 acatctcgct cacgcgcagt caattcttca tgaagttcca tatgattgcg gtggtattca
     4321 accttcttgc taacctcttg ctcaatggcc agctcgccag cagctacctt actgacggca
     4381 tgaagcaatt catctgcact agaagtcttg agcatatagc ctttggcacc agcatctaag
     4441 actggcatga ttttttcatt gtccaaataa gaggtcacaa tcaaaatctt ggcttcaggc
     4501 cattctttaa ggattgctaa ggtcgcgtca atcccattca tctcaggcat gacaatatcc
     4561 atgacaatga catctggacg cagttccaag gccaagtcaa tcccttgaga cccgttggac
     4621 gcctcaccca caacttctac atcgtcttgg aggtcaaagt agcttttcaa gcccaatcgg
     4681 accatttcat ggtcatctac tagtaaaatt ttcatcttta ctcctttatc attccttatc
     4741 taacagggga atacggatat caaccgccag cccttgcttg ggagctgtca agagttgaac
     4801 tgttccagcc atatcttcaa cccgctcctt gatatttcgc agtccataac tcaagtcgtc
     4861 taagctccct aactggaaac caatcccatt gtccaccacc ttcagttgca attcaacatc
     4921 tgtctgatag aggtagacat ctaggcaaga tgcctgggca tggcggaggg tattgctaat
     4981 caactcttgc aggatacgga agatatgctc ctcgattttc ttaggcaatt tcgtcatatt
     5041 ctgcttgaga ctaaccctaa gatcactctt gtcctcaagc tcttttaaga gaatctgaat
     5101 cccttctatc aagctcttct gctccagttc aactggtcgc aaatgcaaga gcaaaacccg
     5161 caaatccttc tgggctgttt ctaaaatagc tgtgacactc tgcaactgga tctgcatctt
     5221 ttctctatcc aatttcaaag cctgctgact gatacccgat aaaatcatgt gggccgcaaa
     5281 caactcctga ctgactgtat cgtgcaaatc ccgagcaatt cgcttccgtt ccttctcgat
     5341 gatttcctct tcctgagcaa ggctctgatt ttcagctttt tgaagagctt ctgtcaaaag
     5401 gttaagttta cctgataagg acttgaaact ggcatccaaa tctggatctg caacctgaac
     5461 cacttcttgc cctgccaata aacgcttgag attagcctgc atttttctta gagaaagctc
     5521 ttcgatccct cgccaaaaca gggctaagag acaggttatg gacatgctga aaaccaacaa
     5581 taaaaagaca aatttttctg ttttttcgac atcgtgcaaa aagatagacc agtcaaaatc
     5641 aagtatttcc agcaagctgt gggagaaaaa aaagacaaac aggaaggagg tgagagcaat
     5701 aatgacatag gcttgttttt tcatcctcta accacctcca catcaccaat catagtggtc
     5761 aagaaaatct tgacactctt gttactcttg agatagtctt ttgtttcttg atgatagtgt
     5821 tcattgcgga gggctcgctt gggctggttg aaaaaagtca aatccccata gagacagtta
     5881 acgctgagac tgacttccac atctacaggt acgatgattt tggtcgttcc taccatcttt
     5941 ctgaggataa tgacattgtc atgattggtt aagatgaccc tctccagatg aatagtgtcc
     6001 ttgcccatga agcgaaagag attgatatca tcgaattggc aagtctggta gcttgaaaaa
     6061 tgatgaagat ttccaaacca acgatttttc tccttcttaa ccgtcacgac ctcttcaaaa
     6121 accaaattgg tctgctcttt ttcctggttc atcatcgggt aaagaagaaa gaggctatag
     6181 ataaccgcaa caaaaatagc tagaatcaca aaaggattga gcataacgat gaaaaagaag
     6241 agaatggttg ccgctactaa aagaagatta tttccctctt taccagtgta gtagcgaatc
     6301 aaaagcaaaa agaggaatag tatcagcaga aaacgcgaaa aatgctctga taccatcaaa
     6361 atcagagctc ctgtcagaag acaggcttcg ataaataaaa agattttaaa ttttctcata
     6421 ggttcatcct ctcccttcta ttttatcaca attcaaaaaa gtcacctcag tctgaggatg
     6481 gaaaaaaggc gctggttacg cctttttcat ctgatccttt gcttctttta attttccata
     6541 aagaagatag tctacttttt gtagatctgc tatggtggca cagttaaggg aacacataat
     6601 caagcgtagg tctgctttcc agccttggac aatgccaatc acttcttcaa ctgtgtaggt
     6661 ttcaaccaat tccagaacgg ttcgtgacaa tcccacagac ttagcaccaa aaaccaagca
     6721 cttaatcata tccagcggat tccgaacccc tccactaacc aagagttcga ccttatcttt
     6781 ccattcttgg gcattgagaa gggcctgcat ggtagactga ccccattgat tgaggtaatc
     6841 acgctggcca ctacgacggt tttcgatata ggcaaagctg gtgccaccac gacccgatag
     6901 gtccactgta cgaacaccga attcataggc tctttcgatt gtcttggcat ccattccaaa
     6961 gcccacttcc ttgaggacaa taggaacggg aatttgctcg ctataatctg ctagatgcga
     7021 ttgccagctt ctaaacttcc tttctccctc gggcatgagt aattcctgca tgacattgac
     7081 atgcacttgc aatagaacag gattcatctc ttctacagtc tgaagtccta actcgacagg
     7141 cttgtccaat ccaatattgg ttccaaggag gagattggga tgactagact tgacagaaaa
     7201 agaatcatcc gttggatttt tgagggctgc gctataagaa cccgttacaa ataaaatacc
     7261 acaggattcc gccacctgag ccagcttttg attgatttct cttcccttat tacttccacc
     7321 agtcatggca ttgatataaa aaggaaagtc ccactttcga ccagcaaact ctgtcgaaag
     7381 atcgatttca tccagattgt aaagaggcaa ggaagaatga atcagctcca cctcatcaaa
     7441 gctattatag gaacttttct gctcaagggc atagaggata tgctcgtcct tacgatttgt
     7501 cgtcatgtcc tatcctttct tgatataaga gctcaatccc cagatcggcc caacgatttt
     7561 ttaaggtttt ggttgattgc gcatcaaaac tcagggcgat gccacagtca ccaccaccag
     7621 caccactact cttggcaacg gcctgcaaat cttgactggc ttctttcaac tgtctaagca
     7681 aaggcgtgta aatatctgta ctcaagcctt ctaaaagctt gctggctact tctacttgct
     7741 cgataatctt ttctgatttc ccctgttcca aggcttcctc aaaagaaacc accgtttctt
     7801 ttgaggaagt taaaaaattt tgattgatat tttgcttgat ttgctggacc atgtgactcg
     7861 atacagccac ttccttggtc catcccacta agaaatcaca ttctaaagtt ggtttcactt
     7921 gtgaaattga aaagccccaa tcacgctcca gaactgtcgc caagttttct tcttctaacc
     7981 aagcagccac cttctggcga tcaaatgact ggtagagaac caaatcctct gccacaatac
     8041 aggcaaggtc gcccatggaa ccattgtctc ctcgcttgag caagacagcg ctagtcagct
     8101 tgaacaagag ctcctgatca acagaaacat catacagagc cagtaaagcc ttgacaacca
     8161 agacaacgac gctgccacta gaacctagac caaacttttt cccttctcgt tccattttgc
     8221 cacagatttc tagagaaaaa ggtcttaaat tctgaccacg aacagcgagg aagtctccca
     8281 tcaaagcaat cgtttcttga atcaagctgt agtcaggatt aggccttaag tccactgcga
     8341 aatcaaacat atctgaatag atacggtagc tgtcagaaaa agcaatctca gccctcatat
     8401 agatgagaat atcctttatc aaagctaact gccctggctc taaaatagca tattcacctg
     8461 cccaatagag ttttccgcaa gttttaacag caatcatctt gactcaaatc ctttgttttt
     8521 gacacaatca agcgataacg ctgaccgaaa atttctgaca aatactccaa gtctttctcc
     8581 tgacagaaga ccttaacatt gggaccagca tccatggtaa agtagcaggc ctctcctttc
     8641 tcacgaagct ggcgaacaaa gtccatagcc tcataagagg catccgtcag ataagaaaag
     8701 gctggactag cagtctttgt cgtagcatgc atagccaggg catttttctc cgttaattct
     8761 ccaatcttgg caaaatcatt ttccttgaga taaatcagca tatcctgata gtccttctca
     8821 gactgacgaa cccagtcgtc gaaagtcgtc gaggtttcca cacaaagttt catcccgtca
     8881 cggctagaga ttggtttttt cttgtcctct agcaccaaca taatcatagc tagtttcaag
     8941 tctgtctcta cagggtaaat ttctccacta tccttatccc aggctcctag tggtccataa
     9001 aaactccgag aagaagagcc tgaggcaaat ttggcttcct gtgccaactg acttctatcc
     9061 aatccaagct tgaaataagc attacaagcc ttgaccaggg cggacaaacc actagaactt
     9121 gaggacaggc ccgctgccgt aggcatattg ttttgagtat cgatacggac aaagccctca
     9181 ccagctggac gataacggtc aataatctta ctcatcttgg catgctcgac ctcattttgt
     9241 agctgaccat tgatgtaaaa ttcgtcagct gttacattgg ctggtaaagg cgacaaggtc
     9301 gtctctgtat acatattttc caaagttaga gaaatactgc tagtagcagg caccatctct
     9361 ttttcttttt tctttcccca atatttgata atagcaatat ttgcgtagga acgtactgtt
     9421 acaggctctc tatccatgtc tgaacagctc ctttctcttc taatctttct gctagttctt
     9481 gtgcgtgtgt caaattggtt accaaggcta tgatacaacc tcctagccca ccaccgctca
     9541 tcttggcacc cagagcacca tggctaagag tcgtttcaac caaaaagtct gcctcagggc
     9601 tactgactcc aatttctttt aaatgtaaat gcgcttgact gaggatttgt cccagtcctt
     9661 cagcatcttt ttgtgaaatc gcaacttctg cttgctgggt taattctccc aaggcatgca
     9721 aaaacggtag ggcatccttg cccttatttt gaaccacttg gatggcttca cgagtatgac
     9781 cataaacacc cgtatcggca atcaccaaat aggcggataa atccatctca agttctgtaa
     9841 atcctacgtt cttgataaag cgaataggtt ggtcactgag acaggtctta gcatccaaac
     9901 cactaggatt catatgagca atcatctcag ctcgattgac caagatttct agtacatcat
     9961 gaggcagatc agcctgatag tagtcaaata ccgcacgaat ggccgctatg ctgatagccg
    10021 ctgacgaacc catcccccgt ttctcaggga tagccgagtc aatctcacaa cgaacgcagg
    10081 tttctgtgat atccaaatac tccagtgaag cataaaccgc catggacaag gtatcctcct
    10141 cataaaggcg ccaaggactc tctgcaggaa ctaccttaca ggtcacctcc acctccaaaa
    10201 gaggcaggga aatggcagga taaccgtaaa cgaccgcatg ttcccctatt aaaattatct
    10261 tactatgtgc ctgaccgaca ccaacttttt ttgtcatttt ttccttttac tagacgaaaa
    10321 aacgtcttat ttttcataca agtattaatt ctttcctatc tattttatta tattttcaca
    10381 aaaaaagcga ttgtttccat tcacaatcgc ttctttcatt attgaaccca ttcgccatta
    10441 taattgacaa aatagccatc tacggtcgta ttcactgcca aggcacctga gctataagcg
    10501 tagtaccatc tgccattgac ctggaaccaa cctgtcgtca tagaacgacg aaagaaactc
    10561 cataccatta agtaaagagg aaagtcgtga gggagcatgc gccattgaca acctgtttta
    10621 gtgacgtaca aagtctcatt aacaagtact cgtttcggtc atttataggt gcggtgtttg
    10681 gagaaatagg gttcaatctt agcccattct tgatcgttta aatcagtatc atatactttg
    10741 cgtgtcataa ctctagttta acattttttg tgaatacagg ttctaaataa tcgaccacga
    10801 aaatttctta agtggaaaac gccttatgaa gtatgctacg ggaaagttat gcacttaatt
    10861 tgacaattca agatgtaaaa atat
//
LOCUS       DBERYS010000057        10746 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.057, whole genome shotgun sequence.
ACCESSION   DBERYS010000057 DBERYS010000000
VERSION     DBERYS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.057"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(105..1529)
                     /locus_tag="ACL8LA_001819"
     CDS             complement(105..1529)
                     /locus_tag="ACL8LA_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS1813531.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="ACL8LA_001820"
     CDS             complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="ACL8LA_001820"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGS1813532.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4465..6423)
                     /gene="ligA"
                     /locus_tag="ACL8LA_001821"
     CDS             complement(4465..6423)
                     /gene="ligA"
                     /locus_tag="ACL8LA_001821"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGS1813533.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6516..7694)
                     /locus_tag="ACL8LA_001822"
     CDS             complement(6516..7694)
                     /locus_tag="ACL8LA_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1813534.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7774..8637)
                     /locus_tag="ACL8LA_001823"
     CDS             complement(7774..8637)
                     /locus_tag="ACL8LA_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1813535.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8640..10541)
                     /locus_tag="ACL8LA_001824"
     CDS             complement(8640..10541)
                     /locus_tag="ACL8LA_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1813536.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3233 a         2366 c         1908 g         3239 t
ORIGIN      
        1 gtttcaatct attataatac caaaaagttc gtattttttc tataattatt attattttat
       61 agcaaaaaaa taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa
      121 acaatggatt tgacatttgt catcgcttca atgctatatt taattccttg cactccagct
      181 ccagaacctt tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta
      241 atgtggactg tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt
      301 gtaaagactg atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct
      361 acactagcca cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc
      421 atatcttttg taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt
      481 ggtgtaagag cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg
      541 gctgaagcat tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt
      601 aatttagaaa cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca
      661 atgacacgtt taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt
      721 tgcttggcag catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca
      781 agttccaaca tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt
      841 gaacctgtaa agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct
      901 gaaccacgac ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct
      961 ttagccaaca agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc
     1021 cctgcaatca aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga
     1081 gcaatcgcta gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt
     1141 gctgcctcaa aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca
     1201 gcataacgaa tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt
     1261 gctacttctt tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct
     1321 gttttatgta aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct
     1381 gcacgcgcag cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc
     1441 aattcttctt gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt
     1501 ccatttacta aattctgata tcttgtcaaa ttcttgtcct cctgaaaatg attaagatat
     1561 attttaccaa attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca
     1621 caaacttgat ttttcaaagt atactcaata aaaataaaaa aaatccacta cgtcaaggcg
     1681 aggctaatgt ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa
     1741 taaaaaaact gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa
     1801 acaagggtgt gctactccaa tttcagccta ctataactgt catagattgc tgaaacaaag
     1861 tctaggtaaa agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag
     1921 gaggtcttgt tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg
     1981 ttctttctat tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat
     2041 ttgtctgtgt aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact
     2101 aatatcattt aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt
     2161 tcctgcattt tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc
     2221 tttgccgtga acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac
     2281 atgatggtaa atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg
     2341 gatgacttga cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac
     2401 ttggttcaca ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt
     2461 caactcacca ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc
     2521 cataaaggcc atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat
     2581 gatttgctct gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt
     2641 gtcatgggct tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg
     2701 acttcctagg attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga
     2761 cttgatagca ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa
     2821 accaatattt ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc
     2881 tgttcccata tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct
     2941 ttgacgaatc atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc
     3001 gtcaatatta tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat
     3061 ttcgtgttca ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg
     3121 attcatacga taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa
     3181 ggtatggtta tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac
     3241 catcaccttc aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt
     3301 ttctggcgcg ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc
     3361 atcgtattct ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt
     3421 cttgatgtaa tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc
     3481 acccaagaaa gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc
     3541 acgaatatgc atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc
     3601 cgaaccgtgc ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga
     3661 acgtttgcca tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg
     3721 agggaattgg acttgatact gataagcctt acctaccaaa tcttcctcaa catccaaact
     3781 ccagacaccg attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc
     3841 aaaagtcttc caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc
     3901 tgtcgctgta ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa
     3961 ttctccttgg taaccccaat gatggtcaaa actagcactg ttaatggcct tatcaaaggc
     4021 aaaaggattt tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat
     4081 cctatcatcg ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat
     4141 agaatattct ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg
     4201 tgaacttggg tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa
     4261 atcaattcgt tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg
     4321 atggtaatga atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt
     4381 ctatttcact aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc
     4441 taaactctgt ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg
     4501 gataccaagt tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag
     4561 gtcggtcttt ttagaaatac tacctgtcac tttggcaccc agactttcga gtttactttt
     4621 agcttctgag cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa
     4681 ggccgcatcc gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa
     4741 ttctctgagc agaatttcag agccttctgt cgcaaaataa gtctgaagac ttttggcaat
     4801 cacgccacct agactttcaa tactagccac ttcctctgaa tctgcctgag acagattttc
     4861 aattgaatgg aaatattgaa gtaaaagctg actaaccttg cttccgacat gacgaattcc
     4921 caaaccaaat aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag
     4981 tttagcagcg gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg
     5041 ataaatatcc gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg
     5101 accaaggcct gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat
     5161 ttgagcaggg caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa
     5221 gtcagagtta caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt
     5281 ggactctacc acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt
     5341 atcgtctttt cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct
     5401 aacagtcgta ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt
     5461 acggccaact gtccagtcaa ctgataagag ttgagcttct ttttcttcgg cagggaactt
     5521 gtaggctact gcccactttg gagccttaac tgtaaaacca agttcttctt gacttgctag
     5581 gtcgttgacc ttgattacca ctccatcaat atcgtaaggc agattttccc gttcctgtcc
     5641 tacttcttgg ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt
     5701 gaccacaaaa cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga
     5761 agggctggct tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc
     5821 caactgacgc agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt
     5881 ttcttggcgc acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg
     5941 aactgtgata tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt
     6001 ttctgtgata ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc
     6061 cttttcataa gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg
     6121 atgagccact tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc
     6181 atcctgcaaa ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt
     6241 gccaccaaca cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa
     6301 ctcgaccaac tcacggtaaa ggcggtcata ctcactgtct gcaaccgagg gattatcgct
     6361 ggtatagtac tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt
     6421 cataagacca ttttaccata aaacaagccc tcctcacaaa cgagaagggc ggaaaaaaca
     6481 cttagtttga aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt
     6541 cgaacataaa taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa
     6601 gaagagagat gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga
     6661 atagctaagg taaaaattgt actcagtact cttccaagaa attcgctctc aaccttggtt
     6721 tgtacttgag taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt
     6781 ccagaaaagg aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc
     6841 aaagccagta aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc
     6901 ccaatgatgg aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg
     6961 taaagctgat tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg
     7021 gaagctacca gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc
     7081 ttgatatcta caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc
     7141 tcttttggaa ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt
     7201 agcgtcatat tgagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca
     7261 cctacaacct gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc
     7321 accacttcag ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc
     7381 acaatgttag caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata
     7441 gccagacaaa gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa
     7501 cggtctgaaa taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc
     7561 tgatacattc ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt
     7621 ccataatcat agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact
     7681 gcatagcgat tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa
     7741 ggagcttttt attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg
     7801 ttcctgataa taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctt
     7861 ttgcatctgc ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa
     7921 ataatggaaa acattacgct cataaagctt aatacctttg tcaataatct tctctgtata
     7981 agcctcaaaa tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt
     8041 gagggccaaa atcaaaacta atctcttatg gcgactaatc tcttggtaaa attcctccct
     8101 ctccataact tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt
     8161 accgaaaaga atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac
     8221 cttatccaaa tcatcctgct tcatatcata agtcgcatct ctttgacaaa tcagaccttg
     8281 tagcaaaatc cagttcagct caaaataaag ggaagtcgtc gaactcttag acttttcaag
     8341 ttgttctctt tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat
     8401 ctgtgccatc atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt
     8461 ttcgattgtt acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga
     8521 ctccccaagc tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt
     8581 taaagaataa tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt
     8641 tacacctgat ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt
     8701 ttgtccagct cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt
     8761 tcagaaatgg cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt
     8821 tgtcgcatga gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact
     8881 ggacttgctt cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct
     8941 gttgctttct tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca
     9001 gacaattcca aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaaa
     9061 agcaaggtac catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc
     9121 aagtggttgg tcggctcatc cagaatcaaa aagttattgt tttccataga caatttagct
     9181 aaaagcaaac gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct
     9241 cctgagaaaa ggaaggcccc aagacggttg cggatttcaa cttctggtgt cagtttgaaa
     9301 tcattccaga gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca
     9361 tagtaaccaa cctcaacatt agcgccaaag cgcttttcac ctttgataaa aggaatctgg
     9421 tccacaatag acttgataaa ggttgacttg ccgataccat ttggaccaac gatagcgaca
     9481 gcattcatct tacgaagatc taggttgata ggttgtgaca aaacttcccc gtcataacca
     9541 atagcagtat tttcaacagt caaaacaaca ttgcccgacg ttttttcaga ctggaaggtc
     9601 atgttggctg ctttcttgcc agcttcaggc ttgtccaaac gctccatttt ttctagttgt
     9661 ttacggcgag attgagcacg tttggtcgtt gaagcacgaa ctagattgcg attgacaaag
     9721 tcttccagag cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtagctagc
     9781 ttttgctcct tcaactcaac aaaacgagag taattcccca catagcgatc caaggaatac
     9841 ttggtcaaat ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg
     9901 ataatgaggg caccgctata gtttaccaag taattctcta gccaggagat ggtttcaata
     9961 tccaagtggt tagttggctc gtccaagacc aagagattgg gcttttcaag gagcatttta
    10021 gctagagcca aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata
    10081 gactcgtcaa acttgaatcc attcaaaatc gctcgaatat cagcttcata ggtaaagcca
    10141 cctgcttggc gaaaattttc agataagcgg tcataatctg acatcagttt atccaaatcc
    10201 tcaccagact tttcacccat ctccagctcc atctgacgca gttgtttatc tgtccgacgt
    10261 aagtcatcaa agacatgaag catctcatca tagatggtat tttcagactc aaaacggcta
    10321 tcttgagcta ggtaagacag agaaatatct tttttcttat tgatttctcc actagttggc
    10381 tcctcttctc caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca
    10441 agggcaatcc gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct
    10501 gcaaaagaac gttcaatttt attagcttgt aaaataatca tacaagtagt atagcatgtt
    10561 tccctaaggc attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa
    10621 actaaattat gtatatttta tattagattc cttcactatc ttgttggatt ttctaaccag
    10681 ctaatcttgt ttcaaatagt tatcgcacaa gcctattatt taattctttt cattatttac
    10741 gtacgc
//
LOCUS       DBERYS010000058        10687 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.058, whole genome shotgun sequence.
ACCESSION   DBERYS010000058 DBERYS010000000
VERSION     DBERYS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.058"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(32..1246)
                     /locus_tag="ACL8LA_001825"
     CDS             complement(32..1246)
                     /locus_tag="ACL8LA_001825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1813537.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1402..1696)
                     /locus_tag="ACL8LA_001826"
                     /pseudo
     CDS             complement(1402..1696)
                     /locus_tag="ACL8LA_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1858..2310
                     /locus_tag="ACL8LA_001827"
     CDS             1858..2310
                     /locus_tag="ACL8LA_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGS1813538.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2345..3733)
                     /locus_tag="ACL8LA_001828"
     CDS             complement(2345..3733)
                     /locus_tag="ACL8LA_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1813539.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3799..4761
                     /locus_tag="ACL8LA_001829"
     CDS             3799..4761
                     /locus_tag="ACL8LA_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGS1813540.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4967..5977)
                     /gene="ccpA"
                     /locus_tag="ACL8LA_001830"
     CDS             complement(4967..5977)
                     /gene="ccpA"
                     /locus_tag="ACL8LA_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGS1813541.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLVKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6401..7000)
                     /locus_tag="ACL8LA_001831"
     CDS             complement(6401..7000)
                     /locus_tag="ACL8LA_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1813542.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7001..8098)
                     /locus_tag="ACL8LA_001832"
     CDS             complement(7001..8098)
                     /locus_tag="ACL8LA_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1813543.1"
                     /translation="MLERLKSIDYMYWASLIFMIFPILPVVTGEIPSWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERAEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVSPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8098..8835)
                     /locus_tag="ACL8LA_001833"
     CDS             complement(8098..8835)
                     /locus_tag="ACL8LA_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813544.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHTPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMPLGSWIGAAFLLIVGSVAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYILVFSAVLFGLTIYLQGHKENA"
     gene            complement(8837..9721)
                     /locus_tag="ACL8LA_001834"
     CDS             complement(8837..9721)
                     /locus_tag="ACL8LA_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813545.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9708..9902)
                     /locus_tag="ACL8LA_001835"
     CDS             complement(9708..9902)
                     /locus_tag="ACL8LA_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813546.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(9899..10105)
                     /locus_tag="ACL8LA_001836"
                     /pseudo
     CDS             complement(9899..10105)
                     /locus_tag="ACL8LA_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3264 a         2440 c         1923 g         3060 t
ORIGIN      
        1 taacttcaac ttgaaatttg ggatgcaagc cctatctacg atactgtttc aagaaacgag
       61 tcatcttttc ttggatttgg gctaactcat caacacgagg aaggtaaacg atacggaagt
      121 ggtctggttc ctgccagtta aagcctcgac catgaaccaa gagaaccttt tcctgcttca
      181 agaaatcaag gacaaactgc tcatcatcat cgatacggta catattgcgg tcgatttttg
      241 ggaagatata gagccccgcc ttgggtttaa cggcagacaa acctggaata tcttgaatgg
      301 cattatagat gaaatttctt tgctcgtaga ttcgtccacc aggaagaagc aattcatcga
      361 ctgattggtg accccccaag gaagtttgta cgacttgttg ggccaaaacg ttagagcaaa
      421 ggcgcatatt ggacagcata ttgagccctt cgatatagcc cttaacatga gtcttagggc
      481 cagacaagac catccaaccc acacggaaac ctgctatgcg gtgggatttt gacagaccat
      541 tcatgctgac acagaagaca tctggtgcca agctcgccac aggcgtatgc acatgtccgt
      601 ccattaccat gcggtcatag atttcatccg caaagataat caaatcgttt tgacgggcaa
      661 tctcaataat ctccagcaag agttccttag gataaagggc tccagttggg ttatttggat
      721 tgataaggac aattgccttg gtattggaag tgatttttga cttgatatcg tcaatatctg
      781 ggtaccattc cacagcttca tcacagatat agtgaacggc atttcctcca gctaggctga
      841 cagccgccgt ccatagagga tagtctggca ttggcactaa gacctcatcc ccattgtcca
      901 aaagcccctg catggacatg acaatcaact cactgacacc atttccaagg taaatatcat
      961 caatatctac gtttgggaat ttcttcagct ggcaatactg catgatggcc ttacgggctg
     1021 agaaaatccc tttggagtca gaatagcctt cactatcacg cgcattcata atcaagtcat
     1081 gaatgacctc gtctggcgct gtaaagccaa attctgctgg atttcctgta ttcagacgta
     1141 aaatcttttc tccattcgct cgcatccgca tggcttcttc caatacagga ccacggatat
     1201 cataagcaac atgctctaac ttactagact tgttatattc tttcatcttg tttcctcaat
     1261 tcatcaggat agtaacatta taccactaga aagagaatgc gacaagttta ctgaaatgtg
     1321 ttactagttt cgtatagaag aaaaaccttg ctaaaggcga ggttaagaac gtagagatag
     1381 caggtatttt gcaaagacac atcacatacc aaaagataaa cctagaaaaa agcaagccta
     1441 gaaaggagtc ataaagaaga ttaaacatga ggaaaagccc ccatatcatc aagtaatccc
     1501 aacgcccact tcgttttgca actagaaata agattagatg gtagagtaca tgaaaaacta
     1561 aaaatccaat aaaacttgga taaagaagcg gtaccagact ctctaattga cagatcatga
     1621 tcacttctac caagactgaa actaggataa ttccataata aaggaaattt gatttttgaa
     1681 aagagttttt catcattctc ccttccttca cttctttcct gctatccttt cttttatttt
     1741 atcatatatt tatatagagt ttaagcccta atattctttt gaaaaatact ttttttgctt
     1801 tacttctata gtgaaaaggt ttacaattat agtaagaaaa tttcaggagg tttcattatg
     1861 gcacaacgtt accaaaatat catggtcgca atcgatggtt ctaaggaagc ggacttggct
     1921 tttgtcaagg gagttcattc tgctctacga aacgacgcta aactcaccat cgcacatgtc
     1981 attgacacac gcgctctcca aagcgtatcc acctttgatg ctgaagttta cgaagaactc
     2041 caagtcgacg ctgaaagtct gatgaaagag tacgaaaaac gtgctaaaga tgctggggta
     2101 gcagatgttc atatcgtcat tgaaatggga aatccaaaga ccctgctagc acgtactatt
     2161 ccagatgccg aggaagtgga cctcatcctc gttggcgcaa ctggtctcaa cgcctttgaa
     2221 cgcctcttgg tcggctcttc atctgaatac atactccgcc atgctaaggt cgatttgctg
     2281 gttgtgagag aacaagaaaa aaccttataa tcacaaagaa aaggagcccc tagctccttt
     2341 ttgtttacga tttatttctc tctttatggc gttcgtaagc cttgagctgg cgctgcagtt
     2401 cctttttaat agcaggttct ggagcatatt tttcttccca attatctggt tttaagattt
     2461 tatgggtcac tggatcaaaa tgagccttgc catctggaaa aattttcccc atattggcct
     2521 gatggacaat atcaaaaata cgttctgggt ccacccccat caagacaaaa ctgccgtagg
     2581 tgaagtaaag cgtgtcaatc aaggcatcca cttgccctat caaatcttgc tgagcaggtg
     2641 tcttcttggc tactttacct gctgccttat caagggcctg atgaagttgc gatacagctt
     2701 gaccaaaatc ttcttcagaa ggactggctg atcgaacaaa ctccaccaat tcttctattt
     2761 taaaaccagc cctatgggtt gcaccctcta aatcccaagc tcgaggttct tcttgggttc
     2821 gttcatccat catgtggtgg aaggtcttga ccttattgaa atgatagtca cggctgacaa
     2881 aaactttttc tgaagacaaa acaccaaaat gttccaaggc cttgtggatc ccatcttgtt
     2941 gattgctagt ggtaatgtgc ttggcagctt ccttgacact gctactacca tttcccatag
     3001 cgaccgacat accgacgcca gccagcattt caaggtcatt atctgagtca ccaaaagcca
     3061 tgacttggtt gaggtcaaag ccatattctt tcccaactcg gcgaatgcct tctaatttag
     3121 aatttccctg attgatgaca tccgatgcaa aaggattgct acgtgtcaat ttcaagtctt
     3181 caaaatcagc tgccgccttc tcagattctt ctggtgtcat cagcatcaaa acttggtaaa
     3241 taggttgatt catcaggtga agcaggtcct cttccttttg gggaacaacc ttgctgacca
     3301 tgcgattaaa agactgactc accgtccgag ttaaaacaga gggaacgaag cgactaattc
     3361 gttgggaaaa agaacccaga ccaaaggaca tgattttaga acccaacatg gcatccttgg
     3421 tccctaaggc aatctccgtg ccctcttttt tagcatagct aattagatgg cgcaaatgta
     3481 acttggaaat agggctcgtg aacaagactc tgtctttact aaagatatac tggccattat
     3541 aggttaccgc aaaatccaga tccaaatcgt ccatcaattc cttaacaaaa aaaggtcctc
     3601 gccctgtcgc tacgccaact agtacccctt gttctttgac aatcttaatc gcatccttag
     3661 tggatttcaa aacactcttg cgattgttga ccaaggttcc atcgatatca aaaaaaacag
     3721 ctttgacttc catcctatcc caatctcccc ttttgtgata caatgattat accacatttc
     3781 agaaagagtg agtaaatcat gcctaagaaa atccttgttt tacatacggg tggaactatt
     3841 tccatgcagg ccgatgcttc tggcgctgtt gtgacgagtt cagataatcc catgaaccat
     3901 gtgtccaacc cacttgaagg aatccaagtc cacgccttgg acttttttaa ccttccaagt
     3961 ccccatatca aacccaaaca tatgctggtc ctctaccaga aaattaaaga ggaagcagat
     4021 aactacgatg gagtggtgat cacacacgga accgatactt tagaggaaac agcctatttc
     4081 cttgatacca tggaagttcc ccatatgcct atcgttctaa caggagccat gcgtagctcc
     4141 aatgaactcg gtagtgatgg tgtttataat tacctaagtg ctttacgagt ggccagcgat
     4201 gacagggctg ctgacaaagg agttttggtc gttatgaacg atgaaatcca cgctgccaag
     4261 tatgtcacca aaacacatac gactaatgtc agcaccttcc agactccaac acatggcccc
     4321 cttggtctca tcatgaaaca ggaaatcctc tacttcaaaa cagctgaacc tcgtgttcgc
     4381 tttgaccttg atcacataca aggtttagtc cctatcatct cggcttatgc tggtatgaca
     4441 gatgagctga ttgatatgct ggatttagaa cacttggacg gtttgattat ccaagccttc
     4501 ggagctggta atattcccaa agaaacggct caaaaattag aaagccttct gcaaaaagga
     4561 attccagtcg ctctggtatc acgatgcttt aacggtattg ccgagcctgt ttatgcctac
     4621 cagggtgggg gcgtacagtt gcaaaaagca ggcgttttct ttgttaaaga actcaacgcc
     4681 caaaaagctc gcttgaaact cctcatcgcc ctcaatgccg gactaacagg acaggctttg
     4741 aaagactata tggaaggcta atactcttcg aaaatctctg caaaccacgt cacgtcgcct
     4801 taccgtatgt atggttactg acttcgtcag tttcatctac aacctcaaaa cagtgtgttg
     4861 cgcagccttt aaccagccgc ctagtccgct ctatggtatt cattaagtca acatctcttg
     4921 tttaagagca caaaatcagg aaatcttctc gattccctga ttttttctat ttacgttttc
     4981 gtgttgagct acgttctgtc aaaccatgag gtaagagaac ttcacgttct tccaactctt
     5041 ccttatgcat aatcttggtc aacatacgca tactaatggc accaaggtca taaagaggtt
     5101 gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat ttgtgaatca tcactagtaa
     5161 taatttcaaa atcttctggc acagaaacac ccttatcagc caaaccgttc aagactcctg
     5221 ctgccaactc atcacctgtc acaactgctg cagttgcatt tgatgaaatc aaacgctctg
     5281 ctaaggcgta accatcatca tagctatatt tagattcaaa taccaaaccc tcactataag
     5341 tgattcctgc ttttttcaag gtttccttgt agccaactaa acgaacctta ccattgatgt
     5401 catccactag cggaccgcta acgaaagcaa tacgctcatt ttctttaaca aggtaactca
     5461 ctgcatcaat tgttgcttgc ttatagtcaa tattgacact tggcaactgg tgctcaacat
     5521 cgacagttcc tgcgagaaca atcggagtac gtgaacgcga aaattctgag cgaattttat
     5581 ctgtcaagtg ataacccata tagataatgc catctacctg ctttgaaaag agggtattga
     5641 caacagaaac ttctttctcg ttatcttcat cgctattagc taggacaata ttgtacttgt
     5701 acatttctgc aatatcatca atccccttag ccaaactcga aaaataacca ttggtaatat
     5761 ttggaatcac gacaccgaca gtggttgtct ttttacttgc aagaccacgc gcaactgcat
     5821 ttggacgata atccaaacga tcaattacct ctagcacttt tttacgggta ttctctttta
     5881 catttttatt gccattgacc acacggctga ccgtcgccat ggaaacacct gcttcacgag
     5941 cgacatcata aatggttact gtatcatctg cattcattcc ttttcctgtc ctttctatct
     6001 cacacattct tttacaagta gaggtgctga ttgaagctct atatctactt acaaaagtga
     6061 agatgtgaaa atttcgtttt catatttcta cttattccat tctatcacta attgtaaaca
     6121 ctttcaagtg ttttttgaag attgattgaa aaaatttcat agaaaaccta ggtttagctc
     6181 cttgctacca ccttagacta aacaaaaagg aggaaactaa gccctcctaa agttatagta
     6241 aaatgaaata agaacaggat aaatcgatca ggacagtcaa atcgatttct aacaatgttt
     6301 tagaagtaga ggtgtactat tctagtttca atctactata ggtattgttc cattcactac
     6361 cgtcaatttt agcacatagt cttcataaaa tattatatca tcataaccaa ccagattctt
     6421 ttgcgatatt agctgcctct gttcgattac cagcatccag ttttgaaaga atattggtga
     6481 catagtttcg aatagttccg tttgagaggt aaagctgatc tgcgatttct tggttggata
     6541 agccccgggc gattcccttt aagacagcaa tctcttgttc tgtcaacgga ttgggacggg
     6601 tcatcaccac ttccatcaat tcaggcgaat actccttgcg tccttcgagg acggtgtgca
     6661 aggtttgcat gaggtctgca atgcttcgtt cctttaatac ataagcatct actccagcct
     6721 tgaccgcacg ttcaaaatac cctgggcgct tgaaggtcgt cactacaacc acctttgttt
     6781 cgagcttttc tgctcgtatc cactccaaga cttcgagtcc tgtcttaaca ggcatttcta
     6841 cgtcaaggat ggcgatatct acagactcct tttctaatag ttgaattgct tcttgaccat
     6901 tcttggcttg taagacagac tctacatctg cttgaaaggc aagcaactgg cacatggcat
     6961 ctcgcaacat actttgatct tctgcgacta atactttcat ctactttctc tccttataag
     7021 gtagtcgaac ttgcacttcc gttggatgtt tctgactgat tacacttact tctcctgaaa
     7081 atggagaaac acgatctcgg actgtatgga gctcatctcc ttttagaaaa gcaaagccgc
     7141 agccatcatc acttactgtt aaaatgagtt ctttctctgc ccgttctaat ttcaggtagg
     7201 ccttcgacgc tttggcatgt ttgataatat tggtcaccaa ctcaagcaaa atcatggaag
     7261 ccgttgactc caattcttga gtaagactag cagtatctag ttggttatcc gtttccacct
     7321 caattccagc aatttctaac atctttttca cagtctctag ttcggatgtc aaagttctag
     7381 acttaagatt ttccacaatg gttcgcactt cattcatgga atccttactg atctggtgaa
     7441 tttcttttaa ttccttttcc acctgtggat aagcctccat ctgaaataac tgcaaggcta
     7501 aatctgtctt gacactcaac atagcaaagg tatgtcccaa gctatcatgc aaatcctgac
     7561 cgatacgact gcgttcattt tcagcaagca atagatttat ctgggcattt tgcttgacct
     7621 gagcttcttt caaatcctcc acaatacgaa tccgaaccaa gccaaaagtc attaaatcga
     7681 caaaagcaag aattacaagt agatagaata gaaactcaac ttcgattctc tgaaaaatca
     7741 acagttggcc cacaacaagg acttgagcaa gaagaaaagt ccagacatgt aaagacttta
     7801 aaccacctac gctgaaatga taacttaaga gattggatag gaaaaagaaa aaccagatat
     7861 aattaacagc aacaaaggca gtattcccaa ctacataagt cagcatgagg ccccaatata
     7921 gccaagatag gcgctggctc ttagttgtta aaacacccaa atacgccact acaaatagaa
     7981 tatcaatcaa taaatgccag ctaggaatct ccccagtcac tacaggtagg atggggaaaa
     8041 tcataaaaat taaactggcc caatacatat agtctatgct ttttagtctt tcaagcatta
     8101 agcattctcc ttatgacctt gaagataaat ggtcaaacca aacaaaactg ctgaaaaaac
     8161 aagtatataa actgtggctg atagattgat gccaccctcg tttaagaagg ttttgagcaa
     8221 ctccatcaac tgataagttg gtaggcactt cccgattgct tgcatccagt ctggaaataa
     8281 agagatgggc atccagagtc cgcctaaaac agccaagcct agatagagaa gattgcccac
     8341 gacagacatc aactgactag aaggcaagag tgtcaaggtc aagccaagcg ctacaaaggc
     8401 aacacttcct actattagca agaacgcagc cccaatccag cttcctagag gcatatccac
     8461 acctcttaca aaatgcccaa ctgagaaaac aaccagaatt gataccaaat aatcaaccat
     8521 catacttgtt atctttgata gataatattc taccatattt acaggggtat ggcgcaatgt
     8581 tttctgccag ttgttgattt tatcagtatg taagacagct gggaatgaaa acatagctgt
     8641 cgacatcatg gaaaaggctg tcatggagat gaggtaatca cgcataaaat tcgcgagttc
     8701 acctggtgtg tcctgataga taccagaaaa aaataaatag aaagccgtcg gcatccctac
     8761 cgacaataga taatagatta attgtcgttt ggtcaataaa aattctatct tactaagtgc
     8821 tatccatcgt ttcatcttag ttatctccct tttgtgtttc ttcaaagatt gtattcaaca
     8881 aactacgatt attaacttcg atttcttgca tcctacatcc tgcttgggct aatagttccc
     8941 agaaagcatc tgcttctcga gtgactactt gtagagcatc ctgtttttgt gaccagtttt
     9001 caaccaagtt agactgctca atgacttcct tgtatgccag aggaaggata aaatgctttt
     9061 caattccctc actacgcata gctagaggcg tcgtatcacg aatcaactct cccttattta
     9121 aaaccaaaat ccggtcagcc gtatgctcta cctcttcaat ataatgagac gaatagagaa
     9181 tcgtgactcc ttgcgctttt aggtcccgaa cgatttccca aaagcgttga cgagttgaag
     9241 tatccatggc agcagttggt tcatctaaaa agacaagctt tggtcgccca atcaaggtca
     9301 agacaaaaga gaagagacgc ttttgcccgc ctgacaattt ttctgcgaat tgctcttttt
     9361 gttgctggtc aaactgcaat agttgatcga tttcctgatc gctcaaggaa tttggataga
     9421 tacgttgaaa gaaagcaatc aactctttga cctttaattt ctgaacgatg acattttctt
     9481 gaggcagata acctctaata tagtctaact gagaactcgt cactgacaag ccttggatgg
     9541 atacttgacc gcttgtgacc agtttatctc caagcagaca gtccaagagt gtggtcttcc
     9601 cagcaccatt gggcccaatc aaggcgacgc attcaccttc agctacctca aaggaaatac
     9661 ccttcaaaat agccttgccc ttgatgtttt tatttaggct ttctacctta atcatattca
     9721 tgatattctc ctttcaacca ctccattctc ataaggaaaa cgacgaaaat cataaatcca
     9781 aaccccaaag caccacgaat gaattggcga agcaaggttt ggtcaaacca acctgtaaac
     9841 atttccacta accataccaa gagtgacagg ccgataaaga aatagatgat ccctctcttc
     9901 attcctcaaa ctcctttttc acatctccga ctaatttcaa accttctcta acaagccaag
     9961 acatcattcc aaagccagca aagagctccc aaggaaaatg atagaaactc tcatccaatc
    10021 ccgaaaacat gagttaggtc ataactcctg ctactactaa actcactgcg ataatcattt
    10081 tatttctcat ctcttcttcc tccatttcat actacaatta tagtcttttg aaatcagagg
    10141 agacagaagc ttctgtcact agaaaatatg acaaatgtca taaaaaattc tgttcaaaac
    10201 aagcaagata cactatacaa taaaacacaa tcagaaaaat ctaaggcaac ttcctcaaaa
    10261 gagatatcaa acccaattca caccataatg taaactaata cttatttaaa atcaaaaaga
    10321 gtagaaattt ttatcagaca aacacatata tagtgtattg aatctataac agtagggctt
    10381 aaatactaac atatttctat aaattaattt aactttcctg atagagctgt tcatatctta
    10441 tttcaattct ctatattata cgttgaacaa aacccttcta ttgctttctt aaaaatttat
    10501 aagagttata aaatctgtta aatttcaatg tgtataccta aactacggta tttattgaaa
    10561 agactgggga caaaaagtat acgctgccaa aatgaattac tgaaaatcaa aaaagagaga
    10621 accaaactga ttccctctta atgtatataa tatctagttt taaaaataca cactcacata
    10681 tctctgt
//
LOCUS       DBERYS010000059        10605 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.059, whole genome shotgun sequence.
ACCESSION   DBERYS010000059 DBERYS010000000
VERSION     DBERYS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.059"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            168..323
                     /locus_tag="ACL8LA_001837"
                     /pseudo
     CDS             168..323
                     /locus_tag="ACL8LA_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            523..3798
                     /gene="rexB"
                     /locus_tag="ACL8LA_001838"
     CDS             523..3798
                     /gene="rexB"
                     /locus_tag="ACL8LA_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGS1813547.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3795..7445
                     /gene="addA"
                     /locus_tag="ACL8LA_001839"
     CDS             3795..7445
                     /gene="addA"
                     /locus_tag="ACL8LA_001839"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGS1813548.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKANFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7660..8838
                     /locus_tag="ACL8LA_001840"
     CDS             7660..8838
                     /locus_tag="ACL8LA_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813549.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9047..>10605
                     /gene="iga"
                     /locus_tag="ACL8LA_001841"
     CDS             9047..>10605
                     /gene="iga"
                     /locus_tag="ACL8LA_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HGS1813550.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEAPKEYTG"
BASE COUNT         3385 a         2139 c         2245 g         2836 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttta ctatagtaga ttgaaataag
       61 atacgaacag cttgattagg gaaatcaaat taatttctag aaatatttta gcatccacgg
      121 tgtactatta tagtttcaat ttactatagt tcaatcattc tatcaagaat attgaatata
      181 taaaggcacg taatattaaa acttcttctg atatcgaatt agagtccgat gttgatggcg
      241 ataaatcaga caatctacca gtcgacatta agatactggg acaacacatt gaagtgtatt
      301 ccgtacctaa gaaacaatca taaaactcaa agccttgtta ccgaggcttt tttattatgt
      361 gattttcagt agccagactt attctatcta atacaagtaa agctaagtat ttaagattta
      421 gcagatgatg aagaaaagaa aggatggcct aaatatttta acagatttac gaggctaatc
      481 atggtataat atagggtgat gaaaaatttc aacgaggctg agatgaaatt actttatact
      541 gatattcgga cttctttgac agaaattcta acaagagagg cagaagagct agttgcagct
      601 ggcaagcggg tcttctacat tgcccccaac tctctttctt ttgaaaagga acgcgccgtg
      661 ctggaatgct tgtcccagca ggcttctttt tcgattaccg tcacgcgctt tgctcaaatg
      721 gctcgttatc tggtcttgaa tgatttacca gctaaaacta ctcttgatga tatcggtctt
      781 gggttggcct tttacaaatg ccttgccgaa ctcgatccca aggacttgcg tgtttatggc
      841 gctattaagc aggatcctca attgatccag cagttaattg agctttatca tgagatgacc
      901 aaatcccaga tgagtttttt ggacttggag aatttaacag atgaggataa gagggcggat
      961 ttactcttga tttttgagaa agtaacagcc tatcttaatc aaggtcagtt agcccaggga
     1021 agtcagttgt cccatttgat tgaggctatt gagaatgaca aggtaagtag tgattttaat
     1081 caaatcacct tggtcattga cggctttact cgtttttctg ctgaggaaga gcgggttgtg
     1141 gacttacttc acggcaaagg tgttgagatt gttatcgggg cttatgctag taagaaagcc
     1201 tataccagtc cttttagcga gggcaatctc taccaagcca gcgtaaaatt tctccatcat
     1261 ctggcttcta aataccaaac gcctgctcag gactgttctc aaactcatga gaagatggat
     1321 agttttgaca aggcctctcg tttgttggag tcttcttatg acttttcaga actcgctttg
     1381 gatgtcgatg agaaagaccg tgaaaattta caaatctggt cttgtttgac gcaaaaggag
     1441 gagttggagc tagtagcccg tagtattcgt cagaaattac atgagaactc agacctgagc
     1501 tacaagcatt ttcgtattct cttgggggat gtagcttctt accagttatc tctcaaaacc
     1561 atttttgacc agtatcagat tcctttttat cttggtagaa gcgaagccat ggctcatcat
     1621 cccttgactc agtttgtcga gtctatttta gctttaaaac gttaccgttt tcgtcaggag
     1681 gatttgatta atcttcttag aactgatttg tatactgacc tcagtcagtc tgatattgat
     1741 gcttttgagc aatatatccg ctatcttggt atcaatggct tgccagcctt tcagcaaatc
     1801 ttcaccaaat cccaccatgg aaaatttaat cttgagcgtt tgaatgtcct ccgcctgaga
     1861 attttagcac ctcttgaaac cctctttgcc agccgaaaac aaaaggctga aaacctccta
     1921 caaaaatgga gtgtctttct aaaagaagga gctgtgacca agcagttaca agatttgaca
     1981 accactttgg aagctgtaga acaggaaaga caagccgaag tttggaaggc tttctgccat
     2041 gttttagaac aatttgcgac tgtttttgct ggttcacagg ttagtctgga agacttccta
     2101 gccttgctcc attctggaat gagtttgtcc caataccgta ccattccagc aacagtggac
     2161 actgttctgg tgcagagtta cgatttgatt gcaccattga ctgctgactt tgtctatgct
     2221 attggactaa ctcaggacaa tttaccaaaa atttctcaaa acaccagtct tctgacagat
     2281 gaagaaaggc aaaacctaaa ccaagcgacc gaagaaggcg ttcaattact gattgccagc
     2341 agtgaaaatc tcaagaaaaa tcgctacact atgctttcct tggtcaattc tgctcgtaag
     2401 cagttgttct tgtcggctcc aagccttttt aacgaaagtg aaagtaagga atctgcctat
     2461 cttcaagagt tgatccattt tggatttagg cggagagaga agaggatgaa tcacaaagga
     2521 ctgtctaagg aggatatggg gtcctatcac agtcttttgt ctagtctggt tgcctatcac
     2581 cagcagggtg agatgagcga tactgagcaa gatttgactt ttgtcaaggt tctgtcgcgt
     2641 gtcataggta aaaaactaga tctgcaaggt ctggaaaatc cagctatccc aaccagtcca
     2701 agcagcaaga ccttagccaa ggacaccttg caagctctct atcctgccaa acaggagttt
     2761 tacctgtcta cgtcgggttt gacagagttt tatcgcaatg aatacagtta tttcctacgc
     2821 tacgttttag gcttgcagga ggaattacgt ttgcgtcctg atgcccgtag tcacgggaat
     2881 ttcttgcatc gtatctttga acgcgcctta cagttgccta atgaagattc ctttgaccaa
     2941 cgtctagaac aagctattca agaaaccagt caagaacgcg aatttgaagc tatttatcaa
     3001 gaaagtttgg aagcccagtt taccaaggaa gttttgcttg atgttgcacg gacgactgga
     3061 catattctcc gacacaatcc agccatcgaa accatcaaag aagaagcaaa ttttggtgga
     3121 aaagaccaag cctttattca attagacaat ggacgcagtg tctttgtacg aggcaaggtg
     3181 gaccggattg accgtttgaa agctaatgga gcgataggag tagtagacta caaatccagt
     3241 ctgactcagt tccagtttcc tcatttcttt aatgggctca attctcagtt accaacctat
     3301 cttgctgccc taaaaagaga agggaagcag aactttttcg gcgccatgta cttggaaatg
     3361 gctgaacctg tccaatctct gatggcggta aaaagtctgg caggagcagt ggtagaagcc
     3421 agcaaatcta tgaaatacca agggctcttc ttggaaaaag aaagcagtta tttaggcgaa
     3481 ttttataaca aaaacaaggc taatcaactg acagatgagg aatttcagct cctactggac
     3541 tacaatgcct atctttacaa gaaagctgct gagaagattt tagcaggccg gttcgccatc
     3601 aatccttata ctgaaaatgg cagaagcatt gccccatacg tccagcaaca tcaggctatt
     3661 acaggctttg aagccaatta ccatctgggc caagcccgtt tcctagaaaa attggaccta
     3721 gctgatggca agcgtctggt cggagaaaaa ctcaagcaag cttggtttga aaaaataaga
     3781 gaggagttaa atcgatgaag cttattccct ttttaagtga ggaggagatt caaaaactgc
     3841 aagaagcaga agcaaattcg agcaaggaac agaagaaaac tgccgaacaa atcgaagcta
     3901 tctacacttc tggtcagaat atcctggtct cagcatcggc tggttctgga aagacctttg
     3961 tcatggcaga gcgcattctg gaccaattgg cgcgtggtgt cgaaatttct caactcttta
     4021 tctcaacctt taccgtcaag gctgcaactg aacttaaaga acgtttagag aaaaaaatca
     4081 gcaagaaaat ccaagaaaca gatgatgtcg atctcaaaca acacttgggt cgccagttgg
     4141 cagacctacc caacgctgcc atcggaacca tggactcttt cacacaaaaa ttccttggca
     4201 aacatggtta tctgcttgat attgcaccta atttccgtat tttacaaaac caaagcgagc
     4261 aacttcttct cgaaaacgaa gtctttcatg aggtctttga agcgcattac caaggtaaac
     4321 agaaagagag ctttagtcat ttgctgaaaa actttgctgg gcgtggcaag gacgaacggg
     4381 gtctgcggca gcaagtctat aaaatctatg acttcctcca atccaccagt aatcctcaaa
     4441 agtggctgag tgaatctttc ctcaaaggct ttgagaaagc tgattttacc agtgaaaaag
     4501 aaaaactgac tgagcaaatc aaacaagccc tctgggattt ggaaagcttt ttccgttacc
     4561 atctggataa cgatgccaag gagtttgcaa aggctgccta tttagaaaat gttcagttaa
     4621 ttctggatga aattggctcc ctaaatcagg agttcgatag tcaggcttat caggcagtac
     4681 ttgcgcgtgt tgtcgccatc tctaaggaga aaaacggtcg tgctctgact aatgccagcc
     4741 gtaaggctga tttgaagccc ctggctgatg cctacaacga agagagaaag acccagttta
     4801 ctaaactagg acaattatca gaccagatag cgattctcga ctatcaagaa cgttatcatg
     4861 gagacacttg gaaactagct aaaaccttcc aatctttcat gagcgatttt gtagaggctt
     4921 atcgtcagag aaaacgacag gaaaatgcct ttgaattcgc tgatatcagc cattacacca
     4981 ttgagatttt agagaatttc ccacaagttc gtgagtctta tcaggagcgc ttccatgaag
     5041 tcatggtcga tgagtatcag gataccaacc atattcaaga acggatgctg gaattgttgt
     5101 ctaatggcca caatcgcttt atggtgggag atatcaagca atccatctat cgtttccgtc
     5161 aggcagaccc gcagattttc aatgagaaat tccaacgcta tgcgcaaaat ccccaagaag
     5221 gcaagctcat tctcctcaag gcaaatttcc gtagtagttc agaagtgctg tcagcaacca
     5281 atgatgtctt tgaacgtctc atggaccaag aggtcggcga aatcaactat gataacaagc
     5341 accagcttgt ttttgccaat accaaactga ctcccaatcc agacaacaag gcagaatttc
     5401 tcctctacga caaggacgat acaggtgagg aagaagagag tcaaacagaa acgaaactaa
     5461 caggcgaaat gcgcttagtt atcaaggaga ttctgaaact tcatcaagaa aaaggtgttg
     5521 cctttaagga aattgccctt ctgacctcca gccgcagtcg taatgaccag attctcctcg
     5581 ccctgtctga gtacggaatt cctgtcaaaa ctgacggaga gcaaaacaat tatctccaat
     5641 ccctagaagt gcaagtcatg ctagacactc ttcgtgtcat tcacaatccc ctgcaagact
     5701 acgccttggt tgcccttatg aagtctccaa tgtttggttt tgatgaggat gagctagcac
     5761 gtttgtccct tcagaaagca gaggataaag tccacgaaaa tctctatgag aaactggtca
     5821 atgcacaaaa aatggcaagt agtcaaaaag gcttgattca cacagctcta gctgaaaaac
     5881 taaagcaatt catggatatc ctagcttctt ggcgcttgta tgccaaaacc cactctctct
     5941 atgacttgat ttggaagatt tacaacgacc gtttttatta tgactatgtt ggggctttgc
     6001 cgaatggtcc tgctaggcag gccaatctct atgccctagc actgcgtgct gatcaatttg
     6061 aaaagagcaa tttcaaaggt ttgtcgcgtt ttattcgtat gattgaccaa gtcttagaag
     6121 cccagcacga tttggcaagc gtggccgtcg caccgccaaa agatgcagta gagctcatga
     6181 ccatccacaa gagtaaaggg ctggagtttc cttacgtctt tatcctcaat atggatcaag
     6241 atttcaacaa gcaagactct atgtcagaag tcattctcag tcgtcagaat ggtcttggtg
     6301 tcaaatatat tgccaagatg gagacagggg cagtagaaga ccactatcct aaaaccatca
     6361 aactctccat tcctagtctg acctataggc agaacgaaga ggaattacag ctagcaagct
     6421 attctgagca gatgcgtttg ctgtatgttg ctatgacgcg ggctgagaaa aagctctatc
     6481 ttgtcggcaa gggttctcgt gaaaagctgg aagtcaagca atacccagca gccaaaaatg
     6541 ggaaactaaa tagcaatact agactgcaag cacggaattt ccaagattgg ctttgggcta
     6601 tcagtaaagt gtttactaag gacaagctca actttagtta tcgttttatt ggcgaagatc
     6661 agttgaccag agaagctatc ggagagttgg aaaccaagag tcctctccaa gatagctccc
     6721 aagcagacaa tcgtcagtca gataccatca aagaagctct ggaaatgctg aaggaggtgg
     6781 aagtttataa tactcttcac cgcgcagcta ttgaacttcc tagtgttcaa accccaagtc
     6841 aaatcaagaa attctacgaa ccagttatgg atatggaagg tgtcgagatt gctggtcaag
     6901 gtcagtcagt aggcaagaaa atcagcttcg atttgccaga tttttcaacc aaagaaaagg
     6961 taactggagc tgagattggt agtgctactc acgaactcat gcagagaatt gacctcagcc
     7021 agcaactaac ccttgctagc ctaacagaaa cactcaaaca agttcaaact agccaagctg
     7081 tcagagacaa gatcaatctt gataaaattc ttgctttctt tgacacagta ctcggtcagg
     7141 aaattcttgc taataccgac catctctatc gcgagcaacc tttctccatg ctcaaacgag
     7201 accaaaagag tcaggaagac tttgttgtcc gtggtatcct tgatggctat ctgctttacg
     7261 aaaacaaaat tgttctgttc gactacaaga cagaccgcta tgatgaacca agtcaactcg
     7321 tagaccgcta tcgtggtcag ttagctctat acgaagaggc tttatcacga gcctattcga
     7381 ttgaaaatat tgaaaaatac ttgattttac tcggtaaaga cgaggttcaa gttgtaaaag
     7441 tataacttat actctttgaa aatctcttca aaccacgtca gcttcgcctt gctgtatata
     7501 tggttactga cttcgtcagt tctatccaca acctcaaaac agtgttttga gctgacttcg
     7561 tcagttctat ctacaacctc aaaacagtgt tttgagctga ttgcggctag ctttctagtt
     7621 tgctctttga tttttattga gtattagaaa ggagacctca tgacacttcc agttagaaaa
     7681 tccctgcacg atgcagtttt acaggcttcg aaagcggata cttgggaaca agctaccaag
     7741 gaatggaatg aagtttcctt gatttttaac ggtatcggcc gtagcaattg tgtctgtgga
     7801 aatgccatca aatacgctta tgaactcttt aacggtgtca caggccaacg cctctttcca
     7861 attggtagcg actgtgttcg tcattttcat cgattgtccc ttgaccagca attggaagag
     7921 gaagaaaaac tgctcagaaa ggttgaaaat ctaaccagaa aagctcagaa aaaggaaaaa
     7981 atcaaggtca ataaaagtga ctttgacgag cgacttctaa aatggctctg ggaaaaaggt
     8041 gttttcaaac ccaatcgtgg caatcaattt gcgcctgaga gagactacca gcttttccta
     8101 gaagtctttc agggaggaag ttggaccaag gcggaaccaa agaaaaaggc tcggatggaa
     8161 gaagtccttg aaaagtgtat caaacccttt ttacttggga aatcggatga ccaactctac
     8221 cttgtcaagc taggcaagga gaaaattgac tacgaacaag aactccgtat ccaggcagag
     8281 aaagaacgca agaagaggga taaaatcgcc aagcaatatg cagacaatct ggttctagcc
     8341 atgggacccg cagagcgtgc ttatcaagat tactttggct ttacagagac cctgactcaa
     8401 gaagaacgca agtgggaaaa aattcttttt ggtaaaaata gagcagaacg ggctatcaag
     8461 gccaaacaat accaaaaaga gctggaaaaa gaccaacgaa ttgccagtca ggatccaatt
     8521 gaaagaaaac agaagcagac ttggctcctc aattcctatt ttcgtgagct tcctgaagaa
     8581 aaagctcgat tttctaggct cttattagaa tatcgaaaaa gtggtgaagt acccttttca
     8641 actgaatacc tgtccgatca tctcatcgac tttttctata aaatgaaagc ctttgaattt
     8701 gaaatcgcac cagaacaagt ccgagatttt ctaaaaaaaa gcctgcagga agaccatcgc
     8761 tcatcagcac aaggaagctg gatagaagga attctcctca actgcctcaa accattttta
     8821 gaacgattag tgatatgagt aacacctacc gtgtaaattt catggtaggt gttattgtac
     8881 ccccaaataa tataaaacaa agtatgaaag cgcatctcaa ttttaagact tttttgtaaa
     8941 ttaagagtat aattaaggta tactgccttt tctatagata atagataata cactaatata
     9001 ataaaggtct ttgtaacata tataaatttt atttcaagga ggaataatgg aaaagtattt
     9061 tggtgaaaaa caagagcgtt tttcatttag aaaattatca gtaggacttg tatctgcaac
     9121 gatttcaagt ttatttttta tgtctgtatt agctagttca tctgtggatg ctcaagaaac
     9181 tgcgggagtt cactataaat atgtggcaga ttcagagcta tcatcagaag aaaagaagca
     9241 gcttgtctat gatattccga catacgtgga gaatgatgat gaaacttatt atcttgttta
     9301 taagttaaat tctcaaaatc aactggcgga attaccaaat actggaagca agaatgagag
     9361 gcaagcccta gttgctggtg ctagcttagc tgctctggga attttaattt ttgctgtttc
     9421 caagaaaaag gttaagaata aaacggtatt acatttagta ttggttgcgg gaatgggaaa
     9481 tggtgtctta gtttcagtcc atgctttaga aaatcatctt ttgctaaatt acaatacgga
     9541 ctatgaattg acctctggag aaaaattacc tcttcctaaa gagatttcag gttacactta
     9601 tattggatat atcaaagagg gaaaaacgac ttctgatttt gaagtaagta atcaagaaaa
     9661 atcagcagcc actcctacaa aacaacaaaa ggtggattat aatgttacac caaattttgt
     9721 agaccatcca tcaacagtac aagctattca ggaacaaaca cctgtttctt caactaagcc
     9781 gacagaagtt caagtagttg aaaaaccttt ctctactgaa ttaatcaatc caagaaaaga
     9841 agagaaacaa tcttcagatt ctcaagaaca attagccgaa cataagaatc tagaaacgaa
     9901 gaaagaggag aagatttctc caaaggaaaa gactggggta aatacattaa atccacagga
     9961 tgaagtgcta tcaggtcagt tgaacaaacc ggaactctta tatcgtgacg aaactataga
    10021 aacaaaaata gattttcaag aagaaattca agaaaatcct gatttagcag aaggaactgt
    10081 aagagtaaaa caagaaggtg cattaggtaa gaaagttgaa atcgtcagaa tattttctgt
    10141 aaacaatgaa gaagtttcac gagaaattat ttcaacttca acgactgcgc ctgtttcaag
    10201 aatagtcgaa aaaggtacta aaaaagctca agttataaag gaacaagctg agactggtgc
    10261 agaacataag gaagtacagt ctggagctat tgttgaaccc gcaattcagc ctgaactacc
    10321 agcagctgta ttaaccgaca aaggtgagtc agcagtccaa ccagagttgc cagaagctgt
    10381 agtaagtgat aaaggcgtac cagaagttca accagcgtta ccagaggcag tcatgactga
    10441 caagggtgat ccagagcagg tagcaccact accagaatac acaggggtgc aagccggagc
    10501 gatagtcgag ccagagaaag tagaagcacc gaaggaatac acaggagtgc aggctggagc
    10561 gatagtcgaa ccagagaagg tagaagcacc gaaggaatac acagg
//
LOCUS       DBERYS010000060        10061 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.060, whole genome shotgun sequence.
ACCESSION   DBERYS010000060 DBERYS010000000
VERSION     DBERYS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.060"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            140..352
                     /locus_tag="ACL8LA_001842"
                     /pseudo
     CDS             140..352
                     /locus_tag="ACL8LA_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            252..698
                     /locus_tag="ACL8LA_001843"
     CDS             252..698
                     /locus_tag="ACL8LA_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1813551.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(836..2140)
                     /gene="eno"
                     /locus_tag="ACL8LA_001844"
     CDS             complement(836..2140)
                     /gene="eno"
                     /locus_tag="ACL8LA_001844"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGS1813552.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2304..2750
                     /locus_tag="ACL8LA_001845"
     CDS             2304..2750
                     /locus_tag="ACL8LA_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGS1813553.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2747..3550)
                     /locus_tag="ACL8LA_001846"
     CDS             complement(2747..3550)
                     /locus_tag="ACL8LA_001846"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGS1813554.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3559..3888)
                     /locus_tag="ACL8LA_001847"
     CDS             complement(3559..3888)
                     /locus_tag="ACL8LA_001847"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGS1813555.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3833..4204)
                     /locus_tag="ACL8LA_001848"
     CDS             complement(3833..4204)
                     /locus_tag="ACL8LA_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HGS1813556.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4263..5696)
                     /gene="glgA"
                     /locus_tag="ACL8LA_001849"
     CDS             complement(4263..5696)
                     /gene="glgA"
                     /locus_tag="ACL8LA_001849"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGS1813557.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5693..6832)
                     /gene="glgD"
                     /locus_tag="ACL8LA_001850"
     CDS             complement(5693..6832)
                     /gene="glgD"
                     /locus_tag="ACL8LA_001850"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGS1813558.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6822..7964)
                     /locus_tag="ACL8LA_001851"
     CDS             complement(6822..7964)
                     /locus_tag="ACL8LA_001851"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGS1813559.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(7954..9882)
                     /gene="glgB"
                     /locus_tag="ACL8LA_001852"
     CDS             complement(7954..9882)
                     /gene="glgB"
                     /locus_tag="ACL8LA_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HGS1813560.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3054 a         2222 c         1768 g         3017 t
ORIGIN      
        1 tttctcttac caacattaac cgcaccattg gttattatat taaaacgaag taagaatagg
       61 acaaatcgat caggacagta aaatctaatt ctaacaatgt tttagaagta gaggtgtact
      121 attctagttt caatctacta tattaatgga caatgcaaga ttccatagaa tggggaagct
      181 agaactcttg tgtgaagagt ttgggcataa acttttacct cttcctccct actcgcctga
      241 gtacaatctt attgagaaaa catgggctca tatcaaaaag cacctcaaaa gggtattacc
      301 aagttgcaat accttttatg aggctctttt gtcttgttct tgtttcaatt gactatataa
      361 attgtctaag cgaaacaacc gataagaatt ggcacaaaag cgaccgtatt tttgttacca
      421 atacaggaaa accagttcat agttctatct tgagcaagtc tctccagcga gcaaacgaac
      481 gccttaaaaa accaattccc aaacatctgt cccctcacat cttcagacac accactatta
      541 gcatcttatc agaaaataaa attcctttaa aaacaatcac ggacagggtt ggtcattccg
      601 actctgaagt cactacttcc atctacaccc acgtcacaaa gaacatgaaa gatgaagcaa
      661 tcaatgtact ggataaagtt atgaaaaaga ttttttaaaa agttttgtcc cttttttgtc
      721 ctctaaatac aaaaatagcc cttcggataa aatccgaggg gctagaaacg ttgttaaatc
      781 aacggccgaa cttttgaatt tcatggttcg ggataaaata gttcactgaa ctattttatt
      841 ttttaaggtt gtagaatgat ttcaatccac gatattcagc tacttcacca agttggtctt
      901 cgatacgaag caattggttg tatttagcga tgcggtctgt acgtgaaagt gaaccagtct
      961 tgatttgtcc tgcgttagtt gcaactgcaa tatcagcgat tgttgaatct tcagtttcac
     1021 ctgaacggtg tgatacaaca gcagtgtaac cagcttcttt agccatttcg atagcttcaa
     1081 aagtttcagt aagagtaccg atttggttaa ctttgataag gattgagtta gcagcacctt
     1141 cttggatacc acgtgcaagg tagtcagtgt ttgttacgaa gaagtcgtca ccaacaagtt
     1201 gtactttctt accaagacgt tcagtaagag ctttccaacc atcccagtcg ttttcatcca
     1261 taccatcttc aatagtgatg attgggtatt tgttaaccaa ttcttcaagg tagtcgattt
     1321 gttctgcaga tgtacgaaca gcagcacctt caccttcaaa tttagtgtag tcgtaaactt
     1381 tacgttcttt atcgtagaat tctgatgaag cacagtcaaa tccgataaat acgtctttac
     1441 ctggtacata tccagcagct tcaatcgcag caaggatagt ttcaacacca tcttcagttc
     1501 cttcgaaacg aggagcgaat ccaccttcgt cacctacggc agtttccaaa ccacgtgatt
     1561 taaggatttt cttaagagcg tggaagattt cagcaccgta acgaagggct tctttaaatg
     1621 ttggcgcacc aactggcaag atcatgaact cttggaaagc gattggagcg tcagagtgag
     1681 aaccaccgtt gatgatgttc atcattggag ttggaagaac tttagtgttg aatccaccaa
     1741 ggtagctgta aagtgggatt tcaaggtagt cagcagcagc acgagctaca gcgatagaca
     1801 caccgaggat tgcattcgca cccaatttac ctttgttagg agtaccgtca agtgcaatca
     1861 tagcacggtc aatagcttgt tgatcacgta catcgtagcc aatgatagct tcagcaatga
     1921 tgttgtttac gttgtcaaca gctttttgtg taccaagacc accgtaacga gatttgtcac
     1981 cgtcgcgaag ttcaactgct tcgtgttcac cagtagaagc tcctgatgga accataccac
     2041 gtccgaaagc acctgattca gtgtaaactt ctacttcaag tgttgggtta ccgcgtgagt
     2101 ctaggacttc gcgagcgtaa acatcagtaa taattgacat tttttactct ccttatgagt
     2161 taaatttttt acacctctat aataccttaa aacccctcct ttttcaagaa aaaacgttat
     2221 ctttgtgcaa cttttcctta actttataaa gtaatcgctt tcttttgtct gttttattct
     2281 aacttttatg atatactgtt ttcatgacag atttatcaaa acaattactt gaaaaagctc
     2341 atggtgggtt aaaaataaat ccggatgagc aaagacgcta tcttggtact tttgaggaaa
     2401 gagttcttgg atatgtagat attgacacag caaatagccc tcagttagaa aaaggctttt
     2461 tatttatttt agaaaacctt caggaaaaag caaagccact atttgtgaag atttcaccaa
     2521 ctatcgaatt tgataagcaa gttttctact taaaagaagc aaaagaaact gatagtcaag
     2581 ccaccatagt atctgaagag catattactt ctccttttgg cctggttatt catagcaatg
     2641 caccagttca agtagaagaa aaagaccttc gacttgcttt tccaaaactt tgggaagtta
     2701 aaaaggaaga accagccaaa acatccttat ggaagaaatg gtttagctaa atcttgcaca
     2761 tatttaataa gtgcccaata ttggcagccg tgcgctccag atagagactg gcatttttca
     2821 aactatcttc taaaggttca cttttctcca aaatagaaaa gacagcttgg atattttcaa
     2881 atggtagggg aggtaaatct tcagcaagac taccgcaaat agcaataaca ggaactccaa
     2941 caggggttct ttttgcaaca cctataggcg ctttcccagc aaagctttga ctatcaagtc
     3001 ttccttctcc aacaacaacc aagtcagcat ctgaaacttt cttatcaaag ttgattaagt
     3061 ccaagcaggt atcaattcca gacacgatac ttgcctgagc aaaggcacac aaaccagcag
     3121 caatccccag tcctccatca ttactggccg tgccaccaac accgatataa atatctttaa
     3181 tccctttaga aatgagatgg agaatcaact ctccaatacc acaagtttgg atttgaagtg
     3241 gatttcgttt ctctagcgga atttttccaa gaccaaccaa gtcagctact tcaaatagtg
     3301 ccagttcccc tttttgaaaa tagcgcatgg cttctttttg tccaaaaggg tctgtcactt
     3361 ggatccattt ttcttttagg tcaagagaat gtcggatagc atctacagta ccttctcccc
     3421 catcaccaac agggcagagg agacattcta catctgctat cgattgttgg aagcctcttt
     3481 ttattgcttc agctacctgt tgagctgtca agctttcctt aaacgaatcc ggtgcaatta
     3541 caatcttcat attttccctc attctaaaca gtcaatcaaa ggaagaactt ctaaaaaatc
     3601 cctcttgtca acatgatgtg gtatttcttt tttgagcatt tctttggaac aaaaggcgat
     3661 tcctaactcc gccgacttca acattaatag attattaacc ccatcaccga ttgccaccgt
     3721 tctttcttta gaaagtttta gtttctttct ccatttttcc agagtttctt ttttgacctg
     3781 gggacttata atttgtccaa ctaattttcc tgttaaaaga ccttctttga cttcaagctg
     3841 gttggcagtg aaataggcaa taccaaggga ttttgctaat ctctccaact attggtgtaa
     3901 atccaccaga caccagacca actaggatgc cattcttttg gagaatagag atgaactctg
     3961 ggacatttag cgatagatga attgagttga agacgttatc aaagaccaaa ataggaagac
     4021 cttccaacaa ggacactctt tttcttaaac tgctttcaaa gaccaactct cctcgcattg
     4081 ctcgacttgt aatctgcgaa atttccgcct catgacctgc ctctctccct aaaagatcaa
     4141 tcacttcttc taggattaag gttccatcta catccaaaac acacaagcct tttacttgag
     4201 acatcagttc tcctctctaa acagcctaaa aatcgtatga agtcatcata cgattttatc
     4261 tattaattaa ctaaactatg gtacaagtca aggtatgact tgcaggctgt atcccatgag
     4321 aagtcactct ccatagcttg tttttgtagg tttctccaaa tgtctggatg gtttctatac
     4381 aagtccaatg ctgtttggaa agtccaattt aaccaataag gagatagatt gtcaaagcta
     4441 aagccagtac cgcttccttc gattggattg aaagcgcgaa ctgtatctcg caagcctcca
     4501 acttcatgga ccaatggcaa ggttccataa cgcatagcca tcatttgaga caagccacac
     4561 ggttcaaaac gacttggcat gaggaagagg tcacaagcag cgtagatttc ctgagcaagt
     4621 ttgacatcaa aagtgatatt tgctgatagc ttgtctgggt aaatctgagc aaaccatgag
     4681 aaagctcctt caaaggctgg atcgccagtt cccaaaagaa caatctgaac atcttcttgc
     4741 aagatatggt gaagactttc gaccaccaca tcaaaacctt tttgacgtgt caaacgagaa
     4801 acaattccca ccagtggaac gtctgctcta acaggcaagc caactctttc ttgcaatttt
     4861 gccttatttt tggctttccc agacaaatct tcctgattga aatgatagtc taaaagagca
     4921 tccgtctgag gattataaag atcagcatca atcccattca cgataccaga tactttacca
     4981 gactccattt taagaatctg atccaaatta catccaaact gactagtcat aatttcatga
     5041 gcatagctag gtgaaacggt tgaaacacgg ttcgcataga gaatacctgc cttcatccag
     5101 ttcagacagt tgttccatcg aagggtgcca tcagcgtaac gttcaaggcc aactccaaac
     5161 aaatcaccca acattccttc tgaaaattgt ccttggaatt ctaaattatg aatggttaaa
     5221 actgtttcaa tgtcctcata ggcttgaatc caacagtatt tttccttcaa caagaaagga
     5281 atcatagctg tatggtagtc atgaacatgg agaagatcag gaataaagtc aatcctttcc
     5341 atagcctcaa tggcagccag ttggaaaaag gcaaagcgtt ctccgtcatc aaaatcaccg
     5401 taaacatgac cacggaagaa ataatattga ttgtcaataa agtagaaggt tacaccattt
     5461 aatactgttt tcttaattcc acaatactgt ctacgccaac caacgctcac ctcaaaatga
     5521 agcacatctt caatctgatt tccaaattta gcctctacca tatcatagta gggtaaaatc
     5581 actgcaactt cgtgcccagc ttttaccagt gattttggaa gagcgccaat gacgtctccc
     5641 aaaccacctg tttttgaaaa gggtgcaccc tctgctgcta caaataaaat tttcatgaat
     5701 gaatatcctc tgttacttta gcacctttct taaccacaac tggatgttct gcagttcctc
     5761 gaatcacaac accaagctca acttcaaccc ctttgtccaa gatagcatat tcgacctgag
     5821 ccccttctcc aataacaaca cgagggaaga gcaggctatc tttaaccaag ctatccttat
     5881 ggacatgaat attacgtgat agaacagaat tagctacttg accttcaata atactaccag
     5941 aggcaaactg agaagtgctt accttagatg tattagcata gtaagttggc tcttcgtttt
     6001 tgacctttgt ataaatcttt tggtttggtg agaaaagaga atagaatttt tgtgattcaa
     6061 gcatatcgat attcgcttga taataagatt taacagagtg aatattggct agatagcccg
     6121 tgtactcgta ggcaaaagct ccctctttta cagccaaatc ccgtaaaaca tagcgcaatt
     6181 tctctggatg ttctttttta gcttcttctt ccaagtgttc aatcaaccaa ggtgtatcaa
     6241 cgacaaagat atctgtagac atattgaacg tttcagctgt tgacttgcta tcaaagagtt
     6301 tatgagaaag aacatggtct gtttcatcta catccaagat tgcatttact tctgaaatat
     6361 ctttcttagc tagtttttta taaactacag tgataggctc ttttgttgta ctatgtaggt
     6421 ggaaaacttg gttcaaatca atgttaataa gaacatcgca gttgagggca accgtttggt
     6481 ttgagccaga acgtttcaaa taagtaagaa gctgttggta gtattctttt ccaactgtac
     6541 tactttctac acgggtattg taaattccta gatagtaatg gctaagaagg gttgataagc
     6601 cccactcgcg ccctgaacga atatggtcaa agactgagct gatattatcc tgctggaaaa
     6661 taccaaagac actacgaaca cctgcattag caaggcttga aagtgggaag tcaatcaaac
     6721 gatatttccc accaaatggc aaacttgcta ctggacggtg gtccgtcaat gtcgacatat
     6781 cgtgaaaacc aactgtattt cctaaaatgg cagaatattt atcaatcttc atctgttgct
     6841 acccccacta cttcattata tcctacaact tgtacttcat ctgttccatc aatttcgaca
     6901 ccgtcagaaa taatcgcacc ttcaccaata atggcacgtt taatcttagc tccttgacca
     6961 atgatagctc cactcatgat aactgaatca aggacttccg ctccttcgcg aacttgcgcg
     7021 cctgttgaaa ggatagaatg tttaacagtt ccatcaacga aacatccgtc tacaactaat
     7081 gagtcttcca catgagcatt tgccccgagg aagtttggtg gtgaaatcaa gtttcttgag
     7141 taaatcttcc attgacggtt acgactatcc aaggcatttt ctggagaaat atactccatg
     7201 ttcgcttccc aaagtgactc aatagtacca acatctttcc aataaccact aaattcataa
     7261 gcataaacac tttcacctga ctcaaggtaa tttggaatga catttttacc aaagtctgac
     7321 atgccaacct tgctcttttc agcagcgact aacatattac gaaggcgttg ccaatcaaaa
     7381 atgtagattc ccatagaagc ttttgtagat ttaggttgag ctggtttttc ttcaaattca
     7441 acaatacgat tgttagcatc tgtgttcatg ataccaaaac ggcttgcttc tttaagaggg
     7501 acgtctaaaa ctgctactgt caagctggca ttattatcct tatgagactg gagcatatca
     7561 tcatagtcca ttttgtagat gtgatcccca gacaaaatca agacatactc aggattgaca
     7621 ctgtcgatat agtcgatatt ttggtaaata gcgtgactag tcccctcaaa ccaacgattt
     7681 ccttcacttg cagaataagg ttgaagaata gagacacctg aattaatacc gtctagtccc
     7741 cagcttgaac cattcccaat atggttgttg agagcaagtg gttgatactg tgtaacgacc
     7801 ccaacattat gaatccctga gttggcacag tttgataggg caaagtcaat gatacggtag
     7861 cgcccaccaa attgcacagc tggttttgcg atgctttgag tgagtttacc gagacgagtt
     7921 ccttgcccac cagcaagaat caaagctaac atttcatttt tcattttcta ctcctttttg
     7981 gtttttattt gtgacggttt tagtagattt caagcgacgt ttgattttcc atacacttgc
     8041 tcccatagcc ggtagggtaa aggttaaggt ctgctcataa tctttccata gtccttcttg
     8101 cgtttgaaca gtttgattat gttctttcca aacgcctccc cactcttcca actcagtatt
     8161 ccatacttct tcgtaaattc ctgcaacggg tagtccgatt gtaaaatctt tccgctcaac
     8221 aggtaccatg ttaaagatac agactaacat ttctcccttt ttacccttac gaataaagga
     8281 aagaacactc tggtctcgat tatccgcatc aatgatttca ataccatcat agctggtatc
     8341 gatttcccac agacagcgat gatctttgta aaactggttt agctgagaag tgaaatactt
     8401 catcttagca ttcattgggt cttctaggtt agaccattcc aactgttctt cagatttcca
     8461 ttctaggaat tgaccgtatt cgctacccat gaagagcaat ttcttaccag gatgacaaat
     8521 ttggtacgta tagagattgc gcaagcctgc gaattgattg taacgatctc cccacatctt
     8581 atgcatcata ctcttcttgc catgaaccac ttcatcgtgc gagaatggca agagataatt
     8641 ctccttgaaa acatacataa agctgaaagt caccaggtta aagtcatatt tacgatagat
     8701 cggatcttct tcgtagaaac ggaggatatc attcatccag cccatgttcc atttgtagtc
     8761 aaatcctaga ccaccaatct ctttcattcc cgtaatcttg gtcgcagacg aactttcttc
     8821 tgcaatcatc atcacatctg gatattctaa cttaataacc tcattcaagc gctgaaggaa
     8881 ataataacct tcatagttga gatttccgcc atctttatta ggtgtccatg gagcatcatc
     8941 atagtccaaa tagagcatgt tgctaacagc atccacacga ataccatcca aatgatagac
     9001 atcaatccaa tgcttaatgc aagaaattaa gaaggactgg acttcatttt ttccaaggtc
     9061 aaaattaagg gcaccccaac catggttatg agccttatta tggtcttggt attcaaaagt
     9121 cggtgtccca tcataatagg ctaaggcatc atcgttgatg gtaaagtgac ctggtaccca
     9181 gtccacaata accccaatat tatgggtatg acactcctcg acaaaatctt gaaactcctc
     9241 tggtcggcca taagcatgct ctaaagcgaa gtaacccata agctgatacc cccaactcaa
     9301 gcccaaagga tgggacatca agggcataaa ctcaatatga gtatagttca tttcaacgag
     9361 ataaggaatg agttcatcct tgagctgggc aaaactataa ggactgccat cagaatttct
     9421 tttccatgat ccagcgtgaa cttcataaat attgacagga cgctcttcaa agccccaacg
     9481 ttttcttcgt gccagccaaa gtccatcctt ccatttcttc tcacgaagct ctgttaagat
     9541 tgcccctgtt cctggacgag cctcatacct gacagcaaaa gggtcaatct tcatcagttg
     9601 atgaccattt tgacgtgtga catgatattt gtaaatatgc ccttcttgag ccatattggt
     9661 aaagacttcc cagaccccaa aatcatttct taccattgga atctgatttt caatccagtt
     9721 ggtaaaatca ccaaccaagt gaacagcctg agcattaggt gcccaaacac ggaaggtata
     9781 gccatgctct ccatttagtt cttccctatg tgctcctaga taatgttgga gataaaaatt
     9841 ttcacccgtc ataaaggttt ttaatgcttc tctattatcc atatactccc cttctcctgt
     9901 aagcgttttc tatgttttta ttatactacc tttttagaga agattcaagt aaattactat
     9961 acttctttaa ttattttgaa aatctacaac aagttcactt actcgttcaa ttgtaaatca
    10021 atattttttc aaaaaattgc gaaaactcct ttctttttct a
//
LOCUS       DBERYS010000061         9919 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.061, whole genome shotgun sequence.
ACCESSION   DBERYS010000061 DBERYS010000000
VERSION     DBERYS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.061"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            219..1409
                     /locus_tag="ACL8LA_001853"
     CDS             219..1409
                     /locus_tag="ACL8LA_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGS1813561.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(1730..2611)
                     /locus_tag="ACL8LA_001854"
     CDS             complement(1730..2611)
                     /locus_tag="ACL8LA_001854"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGS1813562.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(2752..4080)
                     /gene="vncS"
                     /locus_tag="ACL8LA_001855"
     CDS             complement(2752..4080)
                     /gene="vncS"
                     /locus_tag="ACL8LA_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGS1813563.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(4077..4733)
                     /gene="vncR"
                     /locus_tag="ACL8LA_001856"
     CDS             complement(4077..4733)
                     /gene="vncR"
                     /locus_tag="ACL8LA_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGS1813564.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(4782..4865)
                     /gene="pep27"
                     /locus_tag="ACL8LA_001857"
     CDS             complement(4782..4865)
                     /gene="pep27"
                     /locus_tag="ACL8LA_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGS1813565.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(5045..6424)
                     /gene="vex3"
                     /locus_tag="ACL8LA_001858"
     CDS             complement(5045..6424)
                     /gene="vex3"
                     /locus_tag="ACL8LA_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGS1813566.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(6476..7123)
                     /gene="vex2"
                     /locus_tag="ACL8LA_001859"
     CDS             complement(6476..7123)
                     /gene="vex2"
                     /locus_tag="ACL8LA_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGS1813567.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(7136..8413)
                     /locus_tag="ACL8LA_001860"
     CDS             complement(7136..8413)
                     /locus_tag="ACL8LA_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813568.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(8770..8940)
                     /locus_tag="ACL8LA_001861"
                     /pseudo
     CDS             complement(8770..8940)
                     /locus_tag="ACL8LA_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(9083..9682)
                     /locus_tag="ACL8LA_001862"
     CDS             complement(9083..9682)
                     /locus_tag="ACL8LA_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813569.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYTTIDKTSDATALLKLKLNNVDNQPATEVLSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     VGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT         2819 a         2143 c         1833 g         3124 t
ORIGIN      
        1 acaacctcaa aacggtgttt tgagcagcct gcgggctagc ttcctagttt gctctttgat
       61 tttcattgag tataaacagc cactctccaa ttcaattgga gagttttttg atgagagtag
      121 atatttatag acctcaattt gacaccatac attttctatg ataaaatgta acaaattcat
      181 ttacaacaaa tcgaaatcta aatgaaatgg aattttctat gaaatcagtc aaaggactac
      241 tctttatcat agctagtttt atcttgactc ttttgacttg gatgaacact tctccccaat
      301 tcatgattcc aggactagct ttaacaagcc tatctctgac ttttatccta gccactcgtc
      361 tcccactact agaaagctgg tttcacggtt tggagaaggt ctacaccgtc cacaaattca
      421 cagcctttct ctccatcatc ctactaatct ttcataactt tagtatgggc ggtttgtggg
      481 gctctcgctt agctgctcag tttggcaatc ttgccatcta tatctttgcc agcatcatcc
      541 ttgtcgccta tttaggcaaa tacatccaat acgaagcttg gcgatggatt caccgcttgg
      601 tttacctagc ctatatttta ggactctttc acatctacat gataatgggc aatcgtctcc
      661 ttacatttaa tcttctaagt tttcttgttg gtagctatgc ccttttaggc ttactagctg
      721 gtttttatat catttttcta tatcaaaaga tttccttccc ctatctaggg aaaattaccc
      781 atctcaaacg cttaaatcac gatactagag aaattcaaat ccatcttagc agacctttca
      841 actatcaatc aggacaattt gcctttctaa agattttcca agaaggcttt gaaagtgctc
      901 cgcatccctt ttctatctca ggaggtcatg gtcaaactct ttactttact gttaaaaatt
      961 caggcgacca taccaataat atctatgata atcttcaagc cggcagcaaa gtaaccctag
     1021 acagagctta cggacacatg atcatagaag aaggacgaga aaatcaggtt tggattgctg
     1081 gaggtattgg gatcaccccc ttcatctctt acatccgtga acatcctatt ttagataaac
     1141 aggttcactt ctactatagc ttccgtggag atgaaaatgc agtctaccta gatttactcc
     1201 gtaactatgc tcagaaaaat cctaattttg aactccatct aatcgacagt acgaaagacg
     1261 gctatcttaa ttttgaacaa aaagaagtgc ccgaacatgc aaccgtctat atgtgtggtc
     1321 ctatttctat gatgaaggca cttgccaaac agattaagaa acaaaatcca aaaacagagc
     1381 ttatttacga aggattcaag ttcaaataag taatcatact ctcctgggat ttcccaggag
     1441 agttttttat agccaaatga aataagaccg gaacgatgga attttggcat tcaaatcaat
     1501 tgataagaat attttatagt ggagtgaaat aggatacgaa caaattgatt aggaaaatca
     1561 aatgaattta tagaaatctt ttagcagtta tgttgtccta ttctagtttc aaaacgctat
     1621 agaagcagca ttgtactagt ctagattcag tttactatac taaaacgagt agcttgaaat
     1681 caaaaaaccc accctcacag gcaggtttta tctgtattat tcagctagat tatgctttac
     1741 cttctgaacc gaatacgtcg atacgttctt caaccgatgc ttggatagct tttacaccgt
     1801 cagccaagaa tttacgtggg tcgaagagtt ttttcttgtc gtattctgct tcgtttgctt
     1861 cgtagtcacg agcaaattta cgagttgcgt tagcgaatgc gatttggcat tctgtgttaa
     1921 cgttaacttt ggcaacacca agtttgatag ctgcttggat ttgctcatca ggaatacctg
     1981 atccaccgtg caatacgatt gggaatcctg gaagagcttc tgtcaatttt tgcaagtggt
     2041 caaggtcaag accttcccag tttactgggt aagggccgtg gatgttaccg ataccagctg
     2101 ccaagaagtc gataccagtt tcaaccattg ctttagcgtc ttcgattgga gccaattcac
     2161 ctttaccgat gattccgtct tcttcaccac cgatagtacc aacttcagct tctactgaga
     2221 tacctttagc gtgtgctttt tcaacaactt ctttagccaa tttaaggttt tcttcaactg
     2281 gaaggtgtga accgtcaaac atgattgaag tataaccaac ttcgatacac tcaagtgcat
     2341 cttcgtagtg accgtggtca aggtggatag ctactggtac agtgataccc attgattcaa
     2401 caaggttagc gatcaagttg cgagcaactt tgtaaccacc catgtattta gcagcaccca
     2461 ttgaagtttg gatcaaaact ggagcttttt tagcttctgc tgcgcgcaag atagcttgag
     2521 tccactcaag gttgtttgtg ttaaatccac caactgcata accgttgtca cgggctgctt
     2581 ggacaaattt ttctgctgaa acgattgcca ttttatcagg cctcctgtat atttttatgg
     2641 gtcatcccat ttacattgtt cattttatca ctttttgcca aaaaaatcta gtttttcccg
     2701 cagtttcgat tgattttctt ctaactccat ctatgtaaac cctttctctc cctagtcttg
     2761 ggcgactttt ggaaaagcta taaagaaggt taaacgattc tcctccatct cgaaacgata
     2821 agctaatttt tcatgttcta atagactctt aaccacaaag agccccatac cagacccctt
     2881 gaccttgcga ctggcattgt cagaaaaaga ctgggctagt ttttcttgtt cctctgagct
     2941 acagctattt tcgataaaaa gttctccttc tctttctcca attcgaacta agccacctgg
     3001 aacagagtgc ttaatggcat tgctgatgag attagaaaga atcaacttca taactgatgg
     3061 gtttagataa gcctgctgat gggtcaaact attgtctatc tggagctctc tttccttggc
     3121 tagcaaggca taatctttga ccagattttg cgtcatctgg aggaggtcaa ttgtttccct
     3181 atcatctcgc aattcctgca cagaagagag ggaaagtatc tgcagaacat ggtgattgag
     3241 ttcatccaca atccccaagg caactcccag atactggtct ctatccttat aacgaccgat
     3301 attctctctc atattttcga ttaggatttt caaactagcc agcggtgttt tcaattcatg
     3361 agaagctcct cgtaggaatt cgaccttcat cttctccagc tggagaatgg cttcattctt
     3421 ttcatgcaag tccgcaataa cagtcaagag atgctggtag aggctattga tttgttcctt
     3481 gagattacct atctcatcct tagaatccac gcgcaatcgc acttgggaat ccaggtccat
     3541 catccgacgg gtcacccgct tgatttccaa aatcggtgca acaatagtcc gggcgtagat
     3601 gtaggccacc aagagggaaa tcagaaagga ggccagcaag gtatagggaa gaaactggag
     3661 actgatttgc tccgcttcct tttgtaaatc catggaagct agaaactgga gaatcatagt
     3721 accaccgtct tgcgttttca cctcgcgctc ctcaataaag agagaggttg tctggcggtc
     3781 tgtgtccaga ggaagactgt ccttgacttc taacttgtcc tcggtcatct cacctttgac
     3841 ggtccccttg atatcactag tctgggaata caagtctaac acttgctcga tactctgcct
     3901 atctttccct tctagggact gggcaatggc tgttgccttt tgaccaatgg tttcctgacg
     3961 atgactcaga taagtcgaag gaaaaagaaa ataaatagct aaatgaaggc agataaccag
     4021 aacactaaat atcgagaagg tatagataaa tatctttgca aataaacctg ttcgtttcat
     4081 tttcgctcca atttataacc aacattgcgc acagtgagga tacaatccaa gtctagcttt
     4141 ttccgcaatt ccttgatata aacatcaata acacggtcaa agggaacctc atctgtcgct
     4201 ttccagacgg catcgataat ctgagaccga gtcaaggccc ggccttcatt tttcactaga
     4261 tagtccagaa tttccaactc tttggcattg ataggcactt cttgacctgc gaggcttgca
     4321 ctgtagcttt caaagtccac cttggtatcc ttgtaagaaa agattcgtcc tgtatcgtag
     4381 tagcgcttga aaatcgcgtc caccctcact tttaagaggg agagagagaa aggtttttcc
     4441 agatagccat ctgccaaaga ggcaaaggca ctcatcttgt attcctcatc ctgaaaagct
     4501 gtcaacatca agacaggaac ctgactggtt ttacgaatct cagctaggac ttctaagccg
     4561 ttaagcttgg gcatctggat atccagtaaa accagggcca cctcatagct agaaaattgc
     4621 tccagagctt cctgaccgtc cgctgcctca atagtttcat agccacaatc cgtcaaataa
     4681 tcactgaccc cctcacggat catctcttca tcttctacaa ttaaaatttt catactttaa
     4741 ctgctctcta ttttttattt ttcttagaat aaatacctac tctattttct attatagtct
     4801 cttgctggcc ttttgtctgt aagcaactga tcactagata aaacgttgtg aaattccttt
     4861 ctcataaatt ccataacttt agtatattat atttaagcac taaagtacaa agaaagcaac
     4921 tgaaagcaat gattttcacc actgctttca gatttatttt gaattgttaa atagctattc
     4981 ctatccacta ttcttgaata gaaacacaag atgcaatctt tattccagac tcattttttc
     5041 aaatttattc accatccagc aagagctctt ttggttgttt tctaaggaga ttgcttgaag
     5101 caagcgccat aacgagaacc actagaacca aggcaaggac aaaaatgatg ataaagtctg
     5161 atgtctgaat ggaaatgtct aggctcgaca aggtcttgct aaagccatct acttctgcac
     5221 cgccaccaag gttagaggct tgagccgcct tactagcctg tttggcaaca cctgaagtca
     5281 cattggcaag gacagtgttt ccaattgcac gggcagtgta attagctagg aagtaagcag
     5341 aaactagagc agggatagca atcaagatag attcggtgat gaattgaccc aagatacttg
     5401 cctgcttgag gccgatagag aggagaattc ccacttcctt gcgacgggcg ttgatccaaa
     5461 ggctgagcaa gagggcaagg aggagaactg agaagctcaa gctaccccag aagaggaggt
     5521 tggccatctt gtacatacca gagatagatt gctcaagagc tgggtagtta gaggagctct
     5581 tgacgagtgt gtagctcttc cagttgatac cactgatgcc attcaactct ttcataacat
     5641 catccaagtt cttgtctgct gttacaaaga aggttgcgtc cccataaatg gctgtgtctt
     5701 ctgtgtatcc ataaagtttt gcagcagtgt gaatgtctgt aatagctgtg ttttcgtaaa
     5761 gttcttgtga gtaggttact gctgacttat tatgaccatc aaagagtccc ttgattgtca
     5821 cttcaactgt ttccttggct cctttttcat tatctgcatc gtagatatta gagtccagtt
     5881 taaccttgtc ccctactttc cagccgtgtt tggctgccaa gtccttgtgc aagaggattt
     5941 tatccttgtc gtcgttggtt aagtgctctc cttcgactag tttataagaa ccagagacaa
     6001 acttgtcttc tttagaggag tcattgacac ctgtaatcat caagctactt ccaaaacgtt
     6061 tggcacgatc agcagtgaga ttcttcttgg tttctggcgt ttcaatcagg tcatatccag
     6121 tcaaatctcc gatagcgttg atacgtttga cataagactc aatggccttg ttttcggtga
     6181 tttttttgat gtcttcaccc ttgatattcc cagcaccgcg aggcgttcct tggttgacgc
     6241 gacgattgat ttgcatggag aagctattgg tgatattttt aaaggtctcc tgagaagcct
     6301 tggcagtagc tcccttgatt gacaagccga ccaaactcaa gctcgccatg aggagaataa
     6361 tcaggaagat gacaatcgat ttgaaaaact tccttgtaac ataggcaaat gcgttgtgta
     6421 acatagattc cctttctaga ttttgtttta atcattctat taaagtaagc tcaaattatt
     6481 tactagtatt gcgcgtttca gtcagtttct tatcctttaa ttcaagtgta atatctgacg
     6541 cttgtgccac ttctttactg tgagttacga caatcacaca tttacctgtt ttctgggcaa
     6601 gtgatttgag tagttcgaca atatctccag cagttttagg atccagattt cctgttggct
     6661 catcagctag aataactgga gcttctgaga ccaaactgcg agcaatggca acacgttgct
     6721 gttgaccacc tgataactgg agaacattcc gcttgatctg gctttcatcc aaaccaagct
     6781 caagaagtgt attcttgctt gcctttttgt tgaccaatcg gatattttcc agcggagaaa
     6841 gataatctat caagttataa ttttgaaaga ccagggaaat atggtgcatg cgatggtaag
     6901 aatagccctt cttacgaata tcctctcctt gaaaaaggat agaaccttca acaggactat
     6961 ctagaccagc aagtagggac aagagtgtgg attttcctgc tcctgactcc ccaataatac
     7021 tgtaaaattt tccgggttca aaattataat tgatctgata taggactgct tcagcagtat
     7081 tcttataacg gtaggtaaca tcttgtaatt gtaataaagt catgatttct ccttcttaac
     7141 taatagatga taaaatttct ttcggtgatt ttctaaataa gaataggaaa caaagggcta
     7201 cagataagca actaagcaga actagaaaaa cataggattc tgcaaaagat aagatgctag
     7261 ttgataaact gcttgctttg gctagtgtat cttgtaagct tgcctgatct ccacttgcta
     7321 gtagagtttg gagtaggtaa gttgtgattc cgtttcctgc aacaaatgct ggaagcaaag
     7381 ctccaagaga taccaaaact acctctaaac agaattgtag gaaaatcgag ctcttgcctt
     7441 ttccaagtgc aagtaaaatc cccacttcat agacccgttc tctcaaccag agagacaaaa
     7501 ccagaattaa ggctccagct cctgctatca acatcccata aaggaagatg gtcaggaagg
     7561 tttggaaagt tgcaactgag tctttgattt gttcaaaagc cttgttttcc ttttcgactt
     7621 ggtagccttg attttccaag gccaagtttt ctacctgctt catgagtccg tccatttcct
     7681 taggattttc tacatagaag cgtgctgcac tgacttgagc ttcactattg cccaaaaggg
     7741 tttggctact ttcatagtct gtaaagactt gattttcact gaagtcagaa gacaagcctg
     7801 tgaatttctc ttgtttttta ccagaaaaga tgccgataat ctcaaactct actgtttgtc
     7861 cttttccaga ttcagactga ccagcatcca agccaatctt gtcatgaagt gaaagaccgt
     7921 tcttcttagc caattcttcg tggataagga ttttcttgga attccctttt tgaaggtgtc
     7981 gcccttcttt tagattgaaa gccgaactgg taaaggttac atccttggat gaatcctcaa
     8041 gagccgttaa gctaaccaag ttattgtctg cagctgataa atcatcacgc tccacgctct
     8101 gctcgccagt cactgcttcc ttgtctttta gttttgcgac cgtctcgagt tcaggagaga
     8161 cattttccag ccccttaatc ttgcttacag atgctaggtc tgacaacttg aatgtctgac
     8221 cattctctat cttcttaata gaaaaagatg tattgagtga tttataaaga ttgctttcta
     8281 ctgttttgtt ggacttcatc agagtcaaac aggctgaaat tccggccaat aagaccaata
     8341 aaatcagaaa taaaataaaa cttctcagtc gctttctgct gacataagcc caagatcttt
     8401 ggattggatt catttgtcac ctccatattt gtaagactat tataaaaccc aaatatgaaa
     8461 tgtttatgaa atacgaaaaa aaaatatcga gtaggggata atctctagcc cctctcatac
     8521 caccgtacgt gccgttcggc atacggcggt tcaactaact tttaacgcat gtcgttcaag
     8581 gtaataatcc aaacacgaaa ccagtccacg tttttcaagg actggttttg atatagcacg
     8641 tttaagtacc gacttctgag ctactatagt agattgaaac tagaatagta cacctctact
     8701 tctaaaatat tgttagaaat cgatttgact gtcctgattg attcgtccta ctcttatttc
     8761 attttactat aattgatagt ggtcgcccca gccagatacc ttatctgcta tccatttagg
     8821 aacccctaac ttaagcaatc cccataatcg tctcgatttc ttcttccatt gcttccagat
     8881 aatcactcgt aggcgagtac gcaagcgctc atctatgcta gtgactatac ttttcatatt
     8941 tataattcat tcctttcgtt tcactcaagg cacaacacag aatgaaaaag tgttgtgctc
     9001 tttattttgt tttataataa tagttagaaa gcctatcact actacaaatc acggggaggt
     9061 gaataagtga gtggtacagc cactacctcg catattttgt cacatcattt aacggtacat
     9121 aataagttgt accatctgaa taagttgcta caatatcatt tgcatgctct ccttcacctt
     9181 tagcaaaggt tggagctcct actggataat ttttatttgc ctctttcaat ttttcaataa
     9241 tggcattttt ttctgtatct tttatattat caggattttt cactaagatt ttgtctggat
     9301 atgtcggttt agcagaaaca atttttactg ttatttcttt tttattcgaa gcacttgtcc
     9361 agtttccagc attatcttta gcatttaatt ttacagtaat tcctgaacta agaacttcag
     9421 tagcaggttg attatcaaca ttattcaact ttaatttcaa aagagctgtt gcatcagacg
     9481 ttttatcaat cgttgtatat aatgataaat tgttattata accagtttct tcatatttag
     9541 ctgtttgtga gctacttgaa acagaactga aattatgccc actacctccc tgattatctt
     9601 caatgcttac gtctaaatga acttccccac tattatttgg cttagcaaca actgttatag
     9661 taaaataaca taaaatttgc ataaatagat tagggaaatc aaagcaactt ctaggaatgt
     9721 tttatactct tcgaaaatca aattcaaacc acgtcagctt caccttgacg tactcaagta
     9781 cagcctgcgg ctagcttcct agtttgcact ttgattttca ttgagtatta gcagtcacag
     9841 tgtactttcc cagcatcaag tcactataac tctgcacata aaaatggaga agatggccat
     9901 cctcttctcc aaatattaa
//
LOCUS       DBERYS010000062         9826 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.062, whole genome shotgun sequence.
ACCESSION   DBERYS010000062 DBERYS010000000
VERSION     DBERYS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.062"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..352
                     /locus_tag="ACL8LA_001863"
                     /pseudo
     CDS             1..352
                     /locus_tag="ACL8LA_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(467..1270)
                     /locus_tag="ACL8LA_001864"
     CDS             complement(467..1270)
                     /locus_tag="ACL8LA_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGS1813570.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1551..2306)
                     /locus_tag="ACL8LA_001865"
     CDS             complement(1551..2306)
                     /locus_tag="ACL8LA_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HGS1813571.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPCFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2376..5027)
                     /locus_tag="ACL8LA_001866"
     CDS             complement(2376..5027)
                     /locus_tag="ACL8LA_001866"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGS1813572.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(5039..6001)
                     /locus_tag="ACL8LA_001867"
     CDS             complement(5039..6001)
                     /locus_tag="ACL8LA_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HGS1813573.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            complement(6003..6560)
                     /locus_tag="ACL8LA_001868"
     CDS             complement(6003..6560)
                     /locus_tag="ACL8LA_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1813574.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            complement(6547..6957)
                     /locus_tag="ACL8LA_001869"
     CDS             complement(6547..6957)
                     /locus_tag="ACL8LA_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGS1813575.1"
                     /translation="MKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISER
                     TQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTY
                     FMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7409..7804)
                     /locus_tag="ACL8LA_001870"
     CDS             complement(7409..7804)
                     /locus_tag="ACL8LA_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS1813576.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            complement(7900..8154)
                     /locus_tag="ACL8LA_001871"
     CDS             complement(7900..8154)
                     /locus_tag="ACL8LA_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGS1813577.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(8164..9498)
                     /locus_tag="ACL8LA_001872"
     CDS             complement(8164..9498)
                     /locus_tag="ACL8LA_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGS1813578.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9498..9731)
                     /locus_tag="ACL8LA_001873"
     CDS             complement(9498..9731)
                     /locus_tag="ACL8LA_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGS1813579.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
BASE COUNT         2842 a         2190 c         1802 g         2992 t
ORIGIN      
        1 aataaagaga ttttaaacaa gcttttgagc tattcagaag acttgaaaca ccactatcag
       61 ctctataata aggaaccaga gaaatttttc ggacttattg aggacaatct taagcaggtt
      121 catcctcttt ttcagactgt ctttaaaacc ttcctcaaag ataaagaaaa gattgtcaac
      181 gcccttcaac tacactattc taatgccaaa ctggaagcga ccaataatct catcaaactt
      241 atcaagcgca atgcctttgg ttttcgaaac tttgaaaact tcaaaaaaac ggatttttat
      301 cgctctgaac atcaaaaaag aaaggacgaa atttgtcctt tctcgaactt agcttttctt
      361 caacccacta caattgacaa agagccaaac tttgatgata tacgttttct tgtgagaata
      421 tttacttcat ttttgcctaa aattcaatgt ttactcagta tttggattat gaaaaatcaa
      481 ggtctaaatc tagatacatt ttttctgaag acaaatcatt ttgaccaccg agcaagagat
      541 tttcaaaaaa agctgttaaa aactcaggac gtcgctgtaa aatctttgca ttatctaata
      601 ccaaggcatc acgaaaatat ttggaatgtt gctgaaatgg tgtattatca atatcaaaac
      661 caaactcacg aagatactga atcaaaaaga ccgttactgt ccgagtgttt ccttcgcgaa
      721 atggatgaat ctgccagatt cctgaaataa aatgctggat ttgtttaacc acatccgcct
      781 gagttagtgt cgcatatgca acttgttttt cctgattaaa atcataatct aaggtcattt
      841 gaatcatgga gtaatcagag tacacaacac tttcaccatt caaaacaggt tcattctttg
      901 tgatattggt ctgacgaaat tgccccaccg gaatagaggg ttcaaatata tcttgaaaca
      961 actccttatg aatagcaagt aaggtcgcag gactaaagct aaagcctctt cgagacaata
     1021 gttctacaat acgtagagaa accaagtctg cctcttccgt acttgcatca atagtatggt
     1081 gataagccgt tgcatcctca taaacctgct cataagtcag ttctccccgg gactgtttct
     1141 cagccaaaga ttccatatac gctgatggca ctagattgtc aactttctgc agaccaaaac
     1201 ctatccgcca taaatcacgc ttcgcttcat aagacaagtt tggattgtca atgttgtaag
     1261 ttggttgcat agaaatatcc tttcaattgt ttttcttcat taccagtaaa tcacgaaatt
     1321 ggttggaaac tggcttaaac tataaaaagt cctcacaata ggttctatga tatatatata
     1381 tatatacttc atgtaaataa taaaagagtt ataatcagca ctactgataa attcaatacc
     1441 gatttcgcca tcttaaaact tagtcaaacg aataccgttg atttccagtt cattaagtcg
     1501 ttgacctgtc aagacttctc gttccttcaa ctggaaaagt taagggggaa ctaatacaaa
     1561 tcctctacgc ttctatattc aatgatttca tttttgataa tattcttttt aaactcaaaa
     1621 tgttttaaag gattatcgat taacactaac tttggaatat catctagatt tgtgaccaag
     1681 gataaaataa aatccgattg aaactccttg actttttcaa attcttttgc agttaaacgc
     1741 acacgcccct tagattttcg gatacctttt acttcggcaa ggtaagaata ctcttggaca
     1801 tctacctgaa agtcatatcc atcaccataa agacgggcat cggttaactg tccaccttgg
     1861 aatttttctt ctttatcaaa atgaaggata aaataattct cagcctccat tcccgtttcc
     1921 tgtagacgtt tgaatttagt tcgaactatt ggtttttcaa cgatggttat gcctgttttc
     1981 ttcccttcac tggcaagtaa cattttaact atttcagcat aactatgaac atcttcattt
     2041 ccaaacatca tatcaattaa atctttacga aaacgataaa cttcagcttt ctgccaccaa
     2101 ccttttcgat tattgtcaaa acaaggatca aataaatcca ttctattttt tacaactcca
     2161 gttgtttcag caattcccag agaaaccaca tagcgataaa actccgactt gctaaagcat
     2221 tgaaattctt ttataaaaag cttgtcaaac ttggcaagac cgtagccaat taaatttaac
     2281 aattcataat gagggtgttt ggacatcact ttctccatca atagatattg atttcattat
     2341 accaaaaaac acggctcatc accgtgtttc tgtgtttatt tcaccaactt cttcatctca
     2401 tcaatacgtg ctacggtcac atcatacttg gcttggtagt cggcttgttt gtctttttct
     2461 ttttggacaa cttctggttt agcgttggcg acgaagcgtt cgttagagag tttcttacca
     2521 accatgtcca gttctttttg ccatttagcg agttctttgt cgagacgagc tagttcttct
     2581 tcaacattga ggagatcggc cagtggcaag aagatttcgg ctcctgtaat aacgcttgac
     2641 atagccagtt caggtgcagg gatggttgat gcgatttcca agtgttctgg atttgtaaag
     2701 cgtttgatat agttgacatt gctgttaaag aaggcttcca agtcactatc gcttgtctta
     2761 acaaggatgg tgataggctt gcttggtgct acatttactt ccgcacgcgc attccgaaca
     2821 gcacgaatta agtctttgag actttccaca ccagtgtgag ccgcaaggtc ttcaaaggct
     2881 agattaacag ttgggtatgc agctgtcacg atagaacctt ctgagatttg tccaaagatt
     2941 tcctctgtca cgaatggcat gattgggtga aggagacgaa ggatcttgtc caaagtgtat
     3001 agtagaacag aacgtgtgat gactttctct tcttcgttat cgctataaag gacttccttg
     3061 gtcaactcaa cataccagtt ggcaaattct tcccaaatga agttgtaaag gatatgacca
     3121 gccacaccaa actcgaactt atcaaagttt tcagtaactt ttgcaatggt ttcgttgaga
     3181 ttgtggagaa tccagcggtc cgtcacatta ccagcctcac ctgttgcaac ttttgtgaca
     3241 ttgtcatgcg ccacatccag cgtcaaacct ccattgttca tgaggatata gcgagaaatg
     3301 ttccaaatct tgttaataaa gttccatgaa gcatccattt tctcataaga gaaacgcacg
     3361 tcttgtcctg gcgcagaacc gtttgaaagg aaccatcgaa gggcatcggc accgtatttc
     3421 tcgataacat ccattgggtc aatcccgtta ccgagagact tagacatctt gcgtccttgc
     3481 tcgtcacgaa tgagaccgtg gataaggacg ttttggaatg gttgacggcc tgtgaattcc
     3541 aatgactgga agatcatacg agacacccag aagaagatga tgtcgtaacc tgttaccaag
     3601 gttgaagttg ggaaataacg tttaaagtct tctgagtcga cttcaggcca gcccatggtt
     3661 gaaaatggcc agagggcaga actgaaccaa gtatccaaga cgtcttcgtc ctgagtccat
     3721 ccgtcacctt ctggagcttc ttcgccgaca tacatttcac catcagcatt gtaccaggca
     3781 gggatttggt gaccccacca aagctgacga gagataaccc agtcgtggac attttccatc
     3841 cattgaagga aggtatcgtt gaaacgaggt gggtagaatt cgaccttgtc ctctgtgtct
     3901 tggttggcaa tggcgttctt agccaattgg tccatcttga cgaaccattg agtagacaag
     3961 cgtggctcaa ctacgacacc tgtccgttct gagtgaccga ctgaatggac acgtttttcg
     4021 attttgacaa gggcaccgat ttcttccaac ttagcaacga ctgccttacg agcttcaaaa
     4081 cgatccatgc ctgaaaattc aaaggcaagc tcattcatag ttccgtcgtc gttcatgacg
     4141 ttgacttgtg gcaagttatg acgttggcca accaagaagt catttggatc gtgggcaggt
     4201 gtgattttca cgacaccagt accaaactca ggatctgcgt gctcatctcc aacgattggg
     4261 atgagtttat tagcgattgg aaggatgacg tttttaccaa tcaagtcctt gtagcgcggg
     4321 tcttctggat taaccgcaac cgcaacgtcc ccaaacatag tctcaggacg agttgtagca
     4381 acttcaaggg cgcgtgaacc atcttccagc atgtaattca tgtggtagaa ggcaccttct
     4441 acatccttgt gaatcacctc aatatcagaa agggctgtgc gagctgctgg gtcccagttg
     4501 atgataaact caccacgata gatccagcct ttcttgtaaa ggtccacaaa gaccttacga
     4561 acagcttttg acaaaccttc gtcaagagtg aaacgctcac gagaatagtc tacagaaagc
     4621 cccatcttgc cccattgttc cttgatggta gtggcatatt cgtctttcca ttcccagacc
     4681 ttcgtcaaga aagactcacg acctaggtca taacgcgtaa taccctcacc acgtaagcgc
     4741 tcctcaacct tagcctgagt cgcaatacca gcgtggtcca tacctggaag ccaaagggta
     4801 tcaaagccct gcatgcgttt ttgacggatg atgatatcct gcaaagtcgt atcccaagcg
     4861 tgaccaaggt gaagtttccc agttacgttt ggtggtggaa tcacgattga ataaggctta
     4921 gccttttgat cgcctgaagg cttgaaaaca tccgcatcaa gccatttttg gtaacgacca
     4981 gcctcaacct cggctggatt gtatttagat gaaagttctt tagacatgtg tgttctcctt
     5041 aattactttt attgacaatt ttaaccaaac caatttttga aagttcaata taaatctctt
     5101 caatttcagc aaatgctgca agtggtgaca tagtataatt tccaggatta tatttaagat
     5161 catacttgct tatctttgaa atataatacc tagagtgttt caattttaat gattccaact
     5221 ttgatttaat atctactaat gtattatggt ttaactttaa aaacaactta ttataattac
     5281 catagagctc tgtaagagca ttaaagtatt ctttcactag agctatagcg tcaatcttag
     5341 atgtttcttg gtttagtttt atcaactccc ctttcaattt tttccattta aagttggaat
     5401 ttaaaagata atctaaatca atatagaaat ctattataac agtctcatca tctgggctaa
     5461 cagcttttac ctcatttatt ggtaagtcag tatgttgcga ataatttctc aaagaatggc
     5521 aaaatcgata agcaaaattg gtatcatact gatagcttgc aaaaccatgt atacaatcaa
     5581 actcctcact atctttggaa tatttttgtt taatttggtt ttcgttaaaa tcgacaaaca
     5641 attttccact ggataataaa tgaatcatga aacgatttac atctgcctct tcaatttcta
     5701 attcctttga ctcaagttta tttttaatac ttaaaaaatc cgagacatct gaaataaaca
     5761 attcgaacat attcttgatt ttttgaaaat tcttttgaat ctcaaaaatt tggtccgaga
     5821 ctgacattat ttccttggtt ggttcaatat cagagatgac aatacgtttt tcattgttgt
     5881 taatttctaa ataagctaat cgctcgataa tatcactatc atttaattca gcatactcaa
     5941 catcaatttg gcgtaactgc ttctcagata gagagcttac ttcctctttt acatcgctca
     6001 tcttattcct cccactcgct cttcagcaag ccatatatca gactgtcaca gcggtttcct
     6061 tgaacatcct ttcggtctct tatgcgagct tcgagggtaa agccaagctt ttccgcgact
     6121 cgttgacttt gaaggttata tccaaagcaa gttagttcaa tcttgtgaag acccaaatct
     6181 ttaaaggcta agtcaatcaa ggcacgcgca gcttctggca cataacctcg cccccaatag
     6241 tctgagtgta aggtatagcc gatttccatc acatcatctt catggcgatg gttgaaatag
     6301 atagagccaa cgatcttatc cgttcctttg acgacaatcc cgtagcctgc tgggagattt
     6361 tccttttgat tacgctccgg aagaatgtgc tccagataat aaatctcatc ttccaaggtc
     6421 ttgactggag gaaaacctgc tggataggcg acctctggca aactagcata gtcaaagata
     6481 tcctcagcat ccgccacagt acggactcgt aaaacgagac gttctgtttc gattttatct
     6541 ggcagctcag ttcttgccat ccttcttcct cgcttttttg atgaaactgc ccttcatatc
     6601 tacacgcttg tccagatagc gataaacgcg ctgatatcca tctcccatga aataggttgg
     6661 ggcaaacagt tgatttttaa aatgtccctt ttcatccagg agttctgggg caacaagtcg
     6721 ctcaagaatc ttggcaaaga tgtggcaaat accgtcttcc tcaacaatcc tatctacccg
     6781 acaatctaaa acaagtggac aggcgtctaa aataggagtc tgagttcgtt cagaaatttc
     6841 ataatgcact cccaaacgtt ccaatttctc ctgatgactg ataaaaccag cctgctccat
     6901 cgcaagcata gaagtttcat cagaaatatt cacagtaaat ttttgatact gtttgatcta
     6961 ctctgcggca ttctctctcg caacgactcc aatcacaacc caatctccta gactataaga
     7021 ggaactacag gtcgtgatgt tatagccaaa attctaatct tgatatccta aaataaaaac
     7081 aggaaaacca taatatagtt tacttgtgtt aaaagattgc ttcataacaa ccccctttga
     7141 ctaagacgta aaagaaaagc cctgccatct acatgacagg gacgaatgtg tttatccgcg
     7201 gtaccaccca atttcgggca gttgcccgca actctcgtct ttaaagcaaa aagacacttt
     7261 tatttcaatt tttcatccat tagcaaccag ttttgacaca tttgtggact tctcagcgcc
     7321 gccactttct gtaaaatgtg ggattcaaaa cacctctaat ggctctattg tagcaagatt
     7381 ttttcggaaa tgcaaggctc aagaaaaatt acttgaactt gatgccattt tctttcaatt
     7441 tcttgatggt agctgggccg attcctttca agtcaaggag ttctttttca gtccagtttt
     7501 tgaaatctga agcagacttg attccttcat catagaaagt cttggcacga tcaagtggaa
     7561 gtccacccaa atttgctgca aaatcttcta cagaattagc tactttttga gcttgctctt
     7621 tggtagcttc tactgcttgt tcaacttttt tagaaacagc cttaccagct gcgcttgctg
     7681 actgttctgc acgcttcacg actttttttg tctcatctac aaccttagtc acagtacttg
     7741 aaaaggcacc tgcgcgacgg aggccatttc gtaattgttt tttacgattg agtttctttg
     7801 acatgataaa atcctttcaa taaaaaatcc ctgctctgac aaggatttaa cataaacatt
     7861 ctatcaagat tttagtgaaa aatcaagaat ctttctcgtt taataatttc gctcaccaaa
     7921 caagccttct ggcaaatcat ggaatccctt gcctactttg aagttttcaa acttaccaag
     7981 caagaactga taagcatcca agcgactctc gtagcgaatc atggcatctt tggcacgctc
     8041 gtcctggtct tcttcgtaaa ctttttggct ttccttgtgc gccatgtcgt taatcatgat
     8101 ttgggtattg acccaagctt caaattcctt cataaattct tgttcgtaac tcattttttc
     8161 tccttattct aatccacgga aatagtccgt atcaatctca cggtagggct ggatatcctg
     8221 aacgtattcc agtacacctt ggaattctcc gttttcatcg tgtactgcag cataggtgat
     8281 gtggacaaac ttgcctcgcg actcagactt gaaccacatt tcatacttgt ccttggtccc
     8341 ctcacgaagt cccttcatga tagccttgac cttgtccaaa tacttaggcg gatgacagag
     8401 ttcgacattg cgcccgactt gggacggcgt ccgtttgaaa atcatctcat cagctggcgt
     8461 attgtcattg taatactgga aaatatcttc tttattgaca aaggtaatct ccatagggag
     8521 atgattgagg atgagattgg cctgctcgac tgaaagatag ccattaccaa aagcctgttg
     8581 actatggcgg tccagcacag cttccttttc cttaggggta aaggtaatgg taaaatggcc
     8641 ttctggcgta tctatgactt gttgaacttg accttctgcc gtatctagct gtacaggctc
     8701 ctctgcaatc ttttcctcaa taaagctctg tcgttctggc acccatttct ctgacggacg
     8761 gatgatggca tagccatagg catcgctctc ctccgcaatc tgaagccagt catcctgagt
     8821 aaaagactca aggagaatca tgaggaggat ggactcttcc ttgaaaatca tactttcaaa
     8881 ctctgtcgca aaagcttcaa aagcttcctt tacactgcta attgacactt ctggtagtga
     8941 cttggctgtc gttagagctg tttgaaagag ttccctaatc tgatcatcca ctccccacat
     9001 aactttggga ggtgaatcgt gtccatagcg ctccatgata ggaaagaaga gttcttcctt
     9061 acgttggtaa tggatgtcaa attgacccac aagtcccatc tgacgcacca aacccttacg
     9121 catctccgcc agcatttcct cgtcttccat agactcatag gtatctaaca atctacgaat
     9181 gcgaatcaag gccgcacgga gagccagatt ttcttctttg aagacacgaa ctgggtgacc
     9241 tggatgctca gtatctgaaa cttcgacacc tttgatagca tttttaaaaa gattggcatg
     9301 gacatcacag agttccataa catcttcaaa ggtgacacct gagtctgagt tcatcagctc
     9361 gtgctccata agggaaatct caatggctga gacacccgca aaggtcgcat caaaacgctc
     9421 ctgaaccgac tcaggagagg cgccattatg caattctaat aaaatatccc gtaggacatg
     9481 aatccgttca tctgccatta gtctaatcca atcacttcgt agccattcgc ttccagtgtg
     9541 cgtacaatct tgtccatagg agttcctgct agcttagaac cttgtttaag tgatacttta
     9601 cgaccaactg tattgcgcat taagggattg gcaaggggtt taaaacccaa ctccactaga
     9661 atttccaaga cttctggatg cttgtccacc acttctgcaa caggaattga cacatcgatg
     9721 atattgtcca tgacgacctc cattgtatgt tctaatactc aatgaaaatc aaagagcaaa
     9781 ctaggaagct agccgcaggt tgctcaaaac agtgctttga ggttgt
//
LOCUS       DBERYS010000063         8409 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.063, whole genome shotgun sequence.
ACCESSION   DBERYS010000063 DBERYS010000000
VERSION     DBERYS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.063"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(627..935)
                     /locus_tag="ACL8LA_001874"
     CDS             complement(627..935)
                     /locus_tag="ACL8LA_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1813580.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(932..1240)
                     /locus_tag="ACL8LA_001875"
     CDS             complement(932..1240)
                     /locus_tag="ACL8LA_001875"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1813581.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(1245..2540)
                     /locus_tag="ACL8LA_001876"
     CDS             complement(1245..2540)
                     /locus_tag="ACL8LA_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1813582.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2558..3967)
                     /locus_tag="ACL8LA_001877"
     CDS             complement(2558..3967)
                     /locus_tag="ACL8LA_001877"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS1813583.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            4125..4853
                     /locus_tag="ACL8LA_001878"
     CDS             4125..4853
                     /locus_tag="ACL8LA_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1813584.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            5313..5507
                     /locus_tag="ACL8LA_001879"
     CDS             5313..5507
                     /locus_tag="ACL8LA_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1813585.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(5629..5795)
                     /locus_tag="ACL8LA_001880"
                     /pseudo
     CDS             complement(5629..5795)
                     /locus_tag="ACL8LA_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5873..7183)
                     /locus_tag="ACL8LA_001881"
     CDS             complement(5873..7183)
                     /locus_tag="ACL8LA_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGS1813586.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7420..8292
                     /gene="nadC"
                     /locus_tag="ACL8LA_001882"
     CDS             7420..8292
                     /gene="nadC"
                     /locus_tag="ACL8LA_001882"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGS1813587.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         2625 a         1469 c         1593 g         2722 t
ORIGIN      
        1 gatagagttg ttcacatctt atttcaattc actatagttt aatttaagag tagtatttac
       61 taaggcccaa ttaaaatcaa agagcaaact agaaaacgag tgccattcag ctcaaaacac
      121 tgatttgaga ttgcagataa gactagcccc ctcattaaca gatttacgat aaaacgatga
      181 caaggtgtgt tgctttttga tttctaaaga gtataatgat agatctctat aaaataagtg
      241 cgaaggaaat gagcttttat agtcctttcg ttttaaaata ctatctcaga tattcttata
      301 tcgacaagaa gtttttgagt cattccctca tcatacatat taaataaata gtggctcatt
      361 caatttttca ctagaataat aagctagtat agtaaactga aataagatat aaacaaataa
      421 attggagctt aacatccatt tccagcaatt ttttagaaac tacagtggac tattctagat
      481 tcaacatatt ataaaaacta gagtaaaaga aaaggattgg atcttgtgta atgcaggatc
      541 caatcctttc aatcattttg tccaactttt ggaggttcct acaatgtagt cgtcattaat
      601 aaagacagat gggaatgaca gtgttcctat ttattttgat agagatcgat gaattcttta
      661 gatagcaact gaataatctc tgttgaagcc atttggtctt ctgcatgcat aaatagcaag
      721 gagaatccta ttttttctcc agtagcttct ttttgtatga gattagagtg aatcttgtgc
      781 gcttctacta aggagtcttc cgcttcttca actttaattt tcgcttcttt taaatttcct
      841 gccttagcta gttggatggc ttcaataaag gatgatttgg ctgctccact attggcaatg
      901 agctgaaaac agatatattc catttcttct gtcatcttat ttctcctatc catgcaagtg
      961 cttgttccag aacttttgct ccattcatca ttccgtaatc ccgcatatca atggtatcta
     1021 cagggatatt tcctgcaatt tctttcacag caagtaactc ataacgaatt tgtggcccaa
     1081 ttagaatgac atctgcttca tggatattct ttttagcttc tgtcattgat tttgcttgga
     1141 tagagatttc aatcccacgt tcagtcgcac tttgttgcat ttttttaaca agcatacttg
     1201 tcgacattcc cgcattacat actaataaaa tttgtttcat aatcttaacc ttccatttct
     1261 tgttcaacaa ctttgtcatt aactttgata aatggaatgt atagaagaac tccaagtgca
     1321 aagatgatga attgaactag aactgctctc acgtcccctg ctgttgctaa ccatgcattt
     1381 aagaatactg gtgtagtcca aggaacttgt ataaatgcag gactcatgaa tcctgtaact
     1441 gttgctaagt agctgattaa aataccaagg actggaactg tgataaatgg aatagctaat
     1501 gaaatgttat aaacgattgg gtaaccgaat aatactggtt cattgatatt gaagatacca
     1561 ggtccaaaag ataatttagc cacgttttta gagacagcat tgcgactcac taagaatgtt
     1621 gctattaata aacataatgt agatccacta ccacccatta aagcgaatgt ttgtatttgt
     1681 gataggttga tgatgtgtgg aatggcttgt ccattatttg ctgcagtgat gttttcagta
     1741 atgttaatta atagtaatgg ttctaggatg gcactgtaaa taactgcttg gtgaatacca
     1801 aatagccata acatatttcc taaagagtaa ataataatga ccccgattaa gcttgtacca
     1861 atatgacgaa ttggttcttg aataaagatt gtaatgattg agattaagtt cattccagtt
     1921 atattgaata ataatgctga aacaacccca aataaggaga tgacggtcat gactggaagt
     1981 aatacgctaa atgatctatt aacagctggt ggaatatttt caccaaggtt catttgtaaa
     2041 gctttaacat ttgataattc aatgaataat tctgttgcaa taatcgctac gataaccccg
     2101 gcgaacattg cgcctgtacc tgtgttgttg aatgaaagaa cacctgaaat gtttacagca
     2161 tcttttgctc cgtcaggaac tacagaaact gtatttggca tcatcacaat taaagaaact
     2221 aatgatagca ttgatgctgc taacgggttt tcgaaatctc tgtttttagc taagaaataa
     2281 ccaaccatta cagcaataat catacctgaa atacttaaag taccgtttgc aattgttatt
     2341 ccccaatatt ggaatcttgt taatgtatcc ccttggaaaa tccacttaaa taccgtgttg
     2401 ttcaaaagaa cgattaaacc tgccaaaata tataatggca ttactgttac gaatgcatct
     2461 cttagggttt ttaaatgaat ttggttccct agtttaccag caaaggtggc aaattggtct
     2521 ataaattttg agtttttcat atgaatatct cctctcttta ctcctcaatt tctacttcaa
     2581 aaccgttatt gtcacttact ttgcgatacc atcttcctga gctcttgatg gttctctttt
     2641 gcgtttctaa atcaacggag ataaatccgt aacgattctt atatgcatta ttccaagacc
     2701 agcaatcaaa tgcggtccaa gcgtggtatc caaaacagtg acttccctct tcaatggctt
     2761 tatgtagcca tcttaaatgt tcttcataaa attcaatacg atacacgtca tcgataactc
     2821 cattttcatc gataaaccgt gcttcgtttt caacacccat tccgttttca ctgataaacc
     2881 atgggatatt accatattct tccttcacaa tcatagcaat atcataaatg gatttcggaa
     2941 aaatttccca accacgatat ggattcatgc gacgctccgg ccattcatac tctttgaagt
     3001 attggtctgg catccagggc gtctgatatt cctcaggatt tgcttgtgct tgaacacgtt
     3061 ttggatggta gtagtttact ccaagaaaat caaccgtatt tgatttcatc agttgaagct
     3121 ctttttcggt atgactccat aacacgccat ctctctctag ctgttttacc aatctttctg
     3181 ggaaagttcc tttaacagct ggattcaaga agactttgtt aaagaagtca tctgtaaatc
     3241 gacttgcttc taagtcttct ggagaattac ttcttggata agcaggtgtc aagtttaaaa
     3301 taatcccaat ctttccatca agttctaatg agcgatatag ttgaatcact tttgcactag
     3361 caaggtttag attatagatg acttgtacgg cctcttttcc ctttcctttt agatttggat
     3421 aatggaaagc atataagtat cctgcttctg gaatgaccat tggctcattg aaagttgtcc
     3481 agtaatgaac cttatctcca aagcatgtga aagcagtctt ggcaaacttc acgaataact
     3541 ccactacatg tttgctttcc caaccaccgt atttttgaag aagttccact ggtaaatcaa
     3601 aatgatgtaa attcatcaca agatccatct ggttcttttt agcttcttca atgatggcat
     3661 tgtagaaagc aatacctttt ggatcaggct cacctgtctc taaattcttg atgagtcgac
     3721 tccattggat ggaagtgcgg aaagaattaa ctccaatttc cttcatcaag tggaaatctt
     3781 cggtgtaagt atgaaaaaag ttactggcta caagaggtcc gacattatcg aaaaaatctt
     3841 ctggatgcgt tttgaaccag tagtccatca cattttcatg tacttttcca tattgacctt
     3901 ctgtctgagg tccgctagaa gctgctcccc accaaaacag attaggaaat actaactttt
     3961 ttttcatgct caacctcctt tctattattc tatacataaa gtatagcata attttaaaat
     4021 ttgtctagac atttttaaaa attgttgata catatcttgt gtttgcctca tttcttgaac
     4081 tttgttatac tactattaga atattataga gagaaccaga ttaaatgagt acaaaatatt
     4141 tatttattta caatgagatt cgtgaaaaga ttctttgtaa taaatatacc atgaacgaac
     4201 aattacctga tgaaatgaca ttagctaaac agtttgcctg tagtcgaatg acgatcaaaa
     4261 aagctttaga cttgttagtt tctgagggct taatttttag aaaacgtggg cagggaacct
     4321 ttgttctctc tcgtggcagc tcaaaaagaa aattaatcgt tccagaaaga gatatccggg
     4381 gactgacaaa aatatctgaa gatgctcatt ctacaattga ctcgaggatt attcacttca
     4441 aattagaatt tgcaaatgaa tttttagcag aaaaactaca ggtcgctttg cagagtccag
     4501 tttataatat ttaccgcctg cgtattattg acggtaaacc ttatgttctg gaacaaactt
     4561 atatgagtac cgatgttatt ccaggtatta ctgaagatat tttacaaaaa tcgatttaca
     4621 attacattga aggaaagtta ggattgcata ttgccagtgc tacaaaaatc ttacgagctt
     4681 cttctagttc agaaaatgag caacattact tgcagctcct tccaacggaa ccggtatttg
     4741 aagtagaaca agtggcttat ttggataacg gaactccgtt tgagtactcg attagtcgtc
     4801 atcgctatga tttatttgaa tttaattctt ttgcattacg acattcctcc taggagaaaa
     4861 tgtgaaaatg aagccaatct tttacagact ctagtttaag aaaaatttaa aacagggcaa
     4921 gaaggtccca tctatgctta aatggtttct cttttctaaa taagatggct ttaaaagagt
     4981 gatcgtttta tccatcatgt tgaaaaatat cttcgtatag cttatagagt aggtactgaa
     5041 attgttcacc tgatctactt cttatagtta tttagtttta aatagtgttt caaatattct
     5101 tacactgacg agaagttttt gagtcttttc ttgtaacaca tatagtatac tgaggttaga
     5161 atagtagact gtgacttcta acaaattgct agaaatgaat ttcaatctcc caatttattt
     5221 gttcaaatat tgttttaata tattgaataa attctgaatc ataatcattt aaaaaaattt
     5281 tattttttta tttttcatta cgaataatat agatgaaggg gaaagagtat gaaaacagaa
     5341 ctgtttcttt tgctattagt tcaaaaggag aaaaaatgaa agtagaaaat atttcgtata
     5401 gggtggatca tcgtatattg tttgataata tttcttttga tacttcgagt tcaggcgtga
     5461 cattaattac tggtaaaaat ggtacaggaa agtcaacttt actatagtag attgaaacta
     5521 gaatagtaca catctacttc taaaatattg ttagaaatcg atttgactat cctgatctat
     5581 ttgtcctgtt cttatttcat ttcactatat ctcaaattga gtatgacgaa gtgcgctccc
     5641 atgtcctggg aacgcacttt cttcatattt ttcatattct tgaatccatc gataaagact
     5701 attgggatga atttttaaag ttgaactaat catttttaca ggatgagatt tacagcagag
     5761 agtttgaagg ctttatcaaa ggtttttctt ggcataatga cttttcctcg tttccactta
     5821 attttgtgtc tactttatta taccaagtcc acccttaagt tagataataa atctaactta
     5881 aggaagctag aaggatgaga atccaggtgg tcaagagtcc caaacttaag ctgatgggga
     5941 cacccagaat aatttgcttt ttgaaggcaa ggccacgttc ctctatattg ggaagtgaga
     6001 gttgaatgag agaaccagct gatgaaaagg gtgagatatt agtagataga gcgccaataa
     6061 cggtggctgt tgtgagtaag tgaatatcaa tctgaggatt ttgagcactg atgatagcaa
     6121 tgatgggaaa gagggctgga gctacaacgg atagggtgga actaaagagt gacatcactc
     6181 cggctatcac acaaaagaac agaggtaacc agaaatgagg aatggttgtt gtcatgaggt
     6241 gccctatcag tgtgactaaa cctgacttga ccgctagaga cattagtaag ctcatgccgc
     6301 agagcatgat aattgtagcc cagggaacct tagctaaaat ggcttcttgc ttccctaatt
     6361 tgagccttaa ggcgaggcag accatgagta ttgagacaaa gccaatatca aatgtttttt
     6421 gataagtagc tatccaggcg atgtttggga aaatgagatg caacaaggga aaaagccaaa
     6481 ccaaaaccat gctgctgatc atgagcaagg tggtttgtct ttgaaccttg ctgaggagtg
     6541 gtggttggtc aatagtcaag gatgagtttg ttcttccctt actatagtga ctgtaacagg
     6601 ataataaaag caagacgatg agtgggtaga taatgctgac gataaagata tgattgccaa
     6661 gtgaaaaagc ttgctcttcc catcccattt gcttaaacag gccttgaaag acaatgcctg
     6721 agctactggt tatcaaatta gcccctcctg aagctcccca attgacggct tgagctccaa
     6781 tcaaagggtg tttgtccgct ttttgacaga gggtaatcgc tagaggacag caaacggcca
     6841 tagtagtgaa aaatccagca cctaaagcag acaaaagggt tgccatcagg tataaaatca
     6901 tgtagagggc gttagggtgg gtgcgtgtgc ggtagagaat gtgttgagcc aaaacatcaa
     6961 gagtaccgtt agttgttgca acgttataaa agagagagac gctaaaaatg gtaaaaaaga
     7021 gtgaggttgg ccaaaaatga agaagttctt tggggcttaa tcccatgaga gtggttgcga
     7081 tgaggtaaga aaaagcaata gccagcaggc caatattgat tttggtgcgg taaccaattc
     7141 caatggctag agcaatggcg ctaatcatta ttaaatgaat cattgaattg tcctttcagt
     7201 tagaatatgg aaataggttg gcaattttgg cgctcaaaag accacctgtt tcctattggt
     7261 acttgactat tatacacaaa aaaaatacaa ttgtcttgac aattgtattg acacttgtta
     7321 gaattaaaaa tgaggtattc aggcttgtga tttcctatgg aagttaatag taattgcctc
     7381 tagtgcttac aagtgatatt aaaaatagag gacttagtga tgtcaatcat ttcaactgat
     7441 ttaacccctt ttcaaataga tgatacattg aaagcagcct tgcgagaaga tgttcattcc
     7501 gaagattaca gtaccaatgc catttttgat catcatggcc aagccaaggt gtcgcttttt
     7561 gccaaggaag ctggtgtttt agcgggacta accgtttttc aaagggtttt taccctattt
     7621 gatgccgagg tgaccttcca gaatcctcat caatttaagg atggggatcg tttgactagt
     7681 ggcgatttgg ttttagaaat cataggctcg gtgagaagtc tcttaacatg tgaacgcgtt
     7741 gccttgaatt ttttacaaca tttatcaggg attgcttcga tgacagctgc ttatgtagaa
     7801 gccttaggcg atgattgcat taaggtattt gatactcgaa aaactactcc taatttacgt
     7861 ctttttgaga aatatgccgt gagagttggc ggtggctata atcatcgctt taatttatca
     7921 gatgctatca tgctaaaaga caatcacatt gcggcagtag gtagtgttca gaaggcaatt
     7981 gctcaagcgc gtgcctatgc cccttttgtg aaaatggtcg aggtggaagt ggaaagcctt
     8041 gctgctgccg aagaagctgc ggcggcgggt gctgatatta tcatgttgga taatatgtca
     8101 ttggaacaga ttgaacaggc cattacccta attgcaggac gttctcggat tgaatgttct
     8161 ggaaatattg atatgaccac tattagccgt tttcgtggtt tagcgattga ttacgtctcc
     8221 agtggtagtt taacccatag tgctaagagt cttgattttt ccatgaaggg tttaacctac
     8281 cttgatgtct aagttgtaaa ataaactaac tttttaaagg atgtctttcc tctagaacga
     8341 gttttatgtc agatagttta aacgcctctt caaatatagt aaaatgaacc aaaaatagta
     8401 cacaatgtg
//
LOCUS       DBERYS010000064         8393 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.064, whole genome shotgun sequence.
ACCESSION   DBERYS010000064 DBERYS010000000
VERSION     DBERYS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.064"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(382..1944)
                     /gene="guaA"
                     /locus_tag="ACL8LA_001883"
     CDS             complement(382..1944)
                     /gene="guaA"
                     /locus_tag="ACL8LA_001883"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGS1813588.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CLYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2086..2784
                     /locus_tag="ACL8LA_001884"
     CDS             2086..2784
                     /locus_tag="ACL8LA_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1813589.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2827..3723)
                     /locus_tag="ACL8LA_001885"
     CDS             complement(2827..3723)
                     /locus_tag="ACL8LA_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1813590.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(3732..4667)
                     /locus_tag="ACL8LA_001886"
     CDS             complement(3732..4667)
                     /locus_tag="ACL8LA_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGS1813591.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4664..5389)
                     /locus_tag="ACL8LA_001887"
     CDS             complement(4664..5389)
                     /locus_tag="ACL8LA_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGS1813592.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5473..6108)
                     /locus_tag="ACL8LA_001888"
     CDS             complement(5473..6108)
                     /locus_tag="ACL8LA_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGS1813593.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6110..6937)
                     /locus_tag="ACL8LA_001889"
     CDS             complement(6110..6937)
                     /locus_tag="ACL8LA_001889"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1813594.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6959..7294)
                     /locus_tag="ACL8LA_001890"
     CDS             complement(6959..7294)
                     /locus_tag="ACL8LA_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HGS1813595.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7295..8011)
                     /locus_tag="ACL8LA_001891"
     CDS             complement(7295..8011)
                     /locus_tag="ACL8LA_001891"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGS1813596.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
BASE COUNT         2539 a         1877 c         1474 g         2503 t
ORIGIN      
        1 aagcagaata tcatttctct ttttagaatc aaaaggacga gattgtggaa tctctacagg
       61 aacaataggt tgtggcatta aaatatcctc caatagtttt actttactaa tagtatacag
      121 gaggaactcc atgaatgata gataccttgt tatgacgcgc ttactatatt agaaagttgg
      181 ctctctaatt aaattaattc tcattatata tatcaaaaga ccgcagtttg aaaactacgg
      241 tctaattttt tgtccaaatg taaatcactt ggaactatta ttcaatttat tctttttagt
      301 aaatgttccc gttttaatcc cagttacagt ttttaatcct ctgtaaccgt tgattttaca
      361 aagcttttaa tttttttgcg attactccca ctcaactgtt gcaggtggtt tacttgtaat
      421 atcgtagacg atacggttaa catgatccac ttcatttacg atacgtactg agattttttg
      481 aagtacttcc catggaattt tggcaaaatc agcagtcata ccatcgatag aagtgatagc
      541 acggattgca atcgtgtagt catacgtacg accgtcaccc ataacaccga ctgaacgaac
      601 gcctgtgtta acagtgaagt attgccaaat atcgcggtca agtccagctt tagcgatttc
      661 ttcacgaaga atagcgtctg attcacgaac ggtttcaagt ttctcttcag tgatttcacc
      721 catgacacgg atagcaagtc ctggtcctgg gaatggttgg cgccatacga tatggtctgg
      781 cataccaagc tctgtaccaa gagcacgaac ttcatccttg taaagagtat tgagtggttc
      841 aatcaattca aactgcatat cttctggaag accacctacg ttgtggtgtg acttgatagt
      901 ttgagctgta tccgtaccag actcgataac atctgtatat aaagtacctt gagcaaggaa
      961 tttcacatct ttgagcttgc ttgcttcgtc atcgaataca tagacaaact cgttaccgat
     1021 gattttacgt ttttgttcag gatcagaaac gccagcaagt ttgtcaagga aacgtttagc
     1081 agcgtctgct ttgacgatat tcaaaccaaa cttaccaccg agcatgtcca taacttgatc
     1141 agcttcgcct ttacgaagaa gaccgtggtc tacgaagata cagatcaatt gatcgccaat
     1201 cgctttttgg agaagaaccc caacgacaga tgagtcaaca ccacctgata gaccaagaag
     1261 gacacgttta tcaccgacgg tttcacgaat ttttttgatc tgcatgtcaa tgaaattatc
     1321 cattgaccag tcacctttag ccttacaaat gttaagggca aagttacgaa ggatatcatt
     1381 tccgtataca gaatgacgaa cttctgggtg gaattggata ccgtaaatgt gtttatctgg
     1441 gttttcgatg gctgcgtata ggcagtcagc tgatgtacct gtacgaacaa agtcagcagg
     1501 aatctcagta accgcatcac catggctcat caaaacagtc tgttcatcag gtgttgattc
     1561 aaaaagcgct gatggtgtgt gagttagggt tgattgaccg tattcacgat ttccagcatc
     1621 acctgcagga acaacttttc ctccaagttt atgggtcaat aactgcatac cataacagat
     1681 tcccaaaatt ggaattccga gttcgaagat ttctgggtca atatcaaatg aaccatcttc
     1741 atatacagaa tttggaccac ctgatagaat aattcctaca ggattgactt cacgaacttc
     1801 agcagctgaa attttatggc tttttagttc tgaaaaaaca ccaatctcac ggatacggcg
     1861 tgaaatcagc tggttgtact ggctaccata gtccaatacg atgatttttt ctacatcttg
     1921 caaatcagtt gaaatgttgc tcatcttttt cctttctcaa aagaaatatc ttttctcaat
     1981 attctatcac aaataagggc gaattaccaa ctaaacaagg cgaagtttga ctttttctag
     2041 tttattgggg tacaatagag aaaagataga aatgaagtga aaaatatgct acctgcttat
     2101 atgaaaatcc atgatcagat aaaaaaggat attgacgagc accgttgggc tattggtgag
     2161 aggcttccca gtgaaagaga tttagctgag cagtttgcgg tcagtcgcat gaccctccgc
     2221 caagccgtat ctctattagt cgaagaaggc gtcttagagc gccgtgtagg aagcggcacc
     2281 tttgtttcca gtactcgagt acaagaaaag atgcgaggga caaccagttt tactgaaatt
     2341 gtcaaatccc aaggtaaagt tccctctagc cagctcattt cctacaaaaa aaccattccc
     2401 aatgagcaag aagttgccaa gctaggaatt tttccaacgg acaatattat ccgaatggag
     2461 cgggtccgct atgccgacca agttccccta gtttatgaag ttgcttctat tcctgaaaaa
     2521 ttcattaagg actttaaaaa agaagaaatc accagtcatt tcttccaaac cttgcaaaaa
     2581 catggctatc gtatcggcaa atctcaacag accatctatg ctcgccttgc taaagaaaag
     2641 attgcccact atttggaagt tgaaaaagga catgctattc ttggattgac ccaggtttcc
     2701 tacctagaag atggtactgc ttttgaatac gtaaaaagtc aatatgtagg cgaacgcttt
     2761 gaattttatc ttgaaaataa ttagattacc acgcaaaaac tctctgtcac ggacaaagag
     2821 tcttttttat ttttctaaga ataaatcttt caaagaaatc aaagttacag caaacaatat
     2881 cattgccata ccaataaaat caatgggata aaattgttca ttcattatta agaaggcaaa
     2941 gaaaaccgcc gatattggct caattgaagc caacaagctt gacttgaccg gtcctatcag
     3001 actggctcct ttaaggaaag ctgtataggc aaagacagtc ccgataagga taatgcctgc
     3061 aaacgcaagg agaaaatcaa gactagtcgg gatatcggcc tgtagaaccc ctgtaaaagg
     3121 aagggcgacc aaacctgcta tgaccatccc cacaccaatg accaagctgc tcccccactt
     3181 tttaatcaag gctatgggta aaatgatata caaagcataa gtcaaggcag aaaagagacc
     3241 ccagaacagg ccagcaggtg tcatggataa ctggtccaac tgcccatgtg ttgcgatcag
     3301 gaaggttcct ccgatggcga atatgatgga aactatctct cccagtgtcg gtgccaccct
     3361 atccttgata cagctataaa ttaaaattcc gacaggacaa acatactgaa gcaccgtcgc
     3421 tgttcccgca ttggtctcct gaatagcaga cagataggcg aattggttga gaaaaagacc
     3481 aatcagagca aaaataagaa gagacagcaa actctttcta tcctttaaaa agaccagtat
     3541 tttatccttt gcagtagcat aagccaagag catgagaatt ccaccagcga ttaaaagacg
     3601 caagttggtc aagaccagag ccgaaattcc gtgtgccatt aggtattggc cactcgttcc
     3661 tgacaacccc caagcaatac cagccacaac tgttagtaaa gtccctttta aactatttga
     3721 catgtctctc cttactcttc tttgcgaatc aagttcatca cttgattgcg gtctaatatc
     3781 cattgagctc tctcgtcctt ttcataggta cgattcgata ggaaaatagt ggcttcttgc
     3841 ttctgacgat tccacatgat aaaggtacct gtatagcccg tatggtctag ccaatctcct
     3901 tccaaattcc atgctaaaga acgttccttg tcatccaaag gagaaaaatt ttgatttaag
     3961 tctcttgcaa aatcatctgc taaatagtgt tctaaaaaga tttgtaaatc ctttatagtc
     4021 gaaaataaac cagcactccc agcatgtcta cccaggagac gagccttggg atcatgcact
     4081 atgcctgcct ctacacctct aactgttgga acagcaagct caactggccc aaacttggtt
     4141 tccgtcattc cccaaggttt ccagacttga tcctttaaaa tcacatccaa atcttgatta
     4201 aaaattcttt ccaaaataaa gcccaacagc aaaaaatgga catccgaata aagaaaggct
     4261 ggctgacttc gtctgttgag atgaaacatc gcttccttta attcaggggc tgttaaaaga
     4321 tcacgattag gaataaaagg atcaaggtct gttgcatgag tcaagagctg gcgaatagtg
     4381 atgtctggat aatcactctc aggtaaaaaa tctattacca gtctatcaat atctaattga
     4441 cctatttccc acaagaaggt acaaactgtg ccaaccccaa caaccttgct gacactagct
     4501 aggtcataaa ctagtcctgc ctcagtctgc aagccatgct ctgggtcact ctggcctaaa
     4561 tagaactctg tccattgatt gtccttaaaa tacgcaaaag aggctccggg ataaattcct
     4621 gcctcgattt gttcttctat ttttttaata atcttggtcc acttcatact tcttcaaacc
     4681 acaattcaac attatttcta tctttacaaa ggaagaattt ttcagactta ggaataaaat
     4741 attcagtaga ctcaaacttc tggcgaagac ttgctatgtc taattcattg accaagaact
     4801 tgagcataga taagtcccag gtaaccgtat tgtccacagt caaatcctgc ccctgagctg
     4861 gaataaaatc aagggatgcc ccaatttcag atgattccaa gaaactttcg atatcagttg
     4921 ggagatgtaa ttccatagaa atctcaaatt tacttaaaga aattgatgcc aaatctgttt
     4981 gaaattcagg cttttctcct acttctacta gacttgctat gtcatcttcc gcatgaatca
     5041 aaatcaaatc atcttctggt gagaaaattt caaaagcgta gccattttga cctttatata
     5101 atcgatgaat cgaatctgtt ttagataaga ttccttcaat ttctaaggga ttttccacct
     5161 tgacaatcaa tctagctagt ttttttcttc cctctacctt acgagtacgc atactgggag
     5221 cttcttctaa aaccagcttt tcaagacccg tttggtcacc tagtgacaga aaggccgatt
     5281 cttctaacaa ggccttcatt ccaagggttt caatataaaa tgtttcattt aattttctat
     5341 tattagcttt taaagtagga ataatccgta caatctgatt tacattcata aattcctccg
     5401 tcacttttta ttttaaagga ttttagaatt ttttacaaga tattatgctc aaatttaaca
     5461 ttatttagct atttagtaaa gatagtcttt caaggatttt gacaaagcct gataaccagc
     5521 aatactgagg tgcagtccat cagttgtata ttcttttttg agttggcctg cttggtctgt
     5581 caaacaatca aatactggca caaattccac ctgcatatag gcagatgcaa gctcttgata
     5641 ggcttgattc cagttctgaa ttttttcatt cgagcggata tagactgcct gctggtactc
     5701 ctctctctca ttgacaggca aaatggaaag caatttaatc tctgtcaatg gataatcgcg
     5761 agcaacggat tgaatgatag cttcgagatt attgagagcc tcattcacag gaacatcctt
     5821 tccgatatca tttgtcccaa tcagaagaaa aattttatct actgctccac catatagatg
     5881 agcatcaagg ttctctagta acagtcctgt ctgatagcca cgaattcctc gattgacaat
     5941 cgtctttgaa gtcccaaata gctcctgtag aggataatac tcgacaatgg aatccccaat
     6001 aaaaagaatg tttggttcta caacagaaat gtgatttagg tgacgatact tagtttgaat
     6061 tttttcttgt tcctttagga gccaattttc taataactgt actgccactt cattctcctt
     6121 tttctaacca gttttccaag acttgataaa ctcctccttg gctgttggct ggtgctagag
     6181 cagtcgccac agctttggct ttctcatcag cattttccat cgcataggca attccagcca
     6241 tttcaagcat ttcaacatca ttttcactat caccaaaagc catgatttct tgggatttca
     6301 agtcccagcg cttgagtaat tcctccaagc cccatgcttt atgaatccca gcttggagga
     6361 tatcaatgca accatagcca ctggatacag ctcggacacg gccatcaaag agagcattga
     6421 tttcttccaa aaccgaactc aaacgttcct caccaacaac catgctcatc ttgagcacac
     6481 caccaaagag gtcagatgtt aattcatcca caaattgcat ccgttggtag aatttttcaa
     6541 tcatttctgg agtcataaaa ctttcaagat ctgtaaaaat cgtaccttcc ttgacaaaat
     6601 cacccttcat ccccgttaca acaaactggt cctgacacgc tcgacccttg aaatgagtca
     6661 aagccttgtt gacaatggcg tcatcccatg tctgagcctg aatcaattca ttgttttcaa
     6721 aaatacgagc gccattagca acaaccagaa ccactcgatc caccaaggga ctcagtagtt
     6781 gtctcatgcg gtgaatttca ttgcccgtcg caatgacaaa acgaatgccc ctttgatcca
     6841 actgatctaa aatcttttcc aatcgtggga gatcaagctg acctctagca tccagcaagg
     6901 tcccatccat atctgtcgca attaccttaa tcatcattta ctttcctctg gattcaagct
     6961 aataccactt caatcccaca tgttcattca tttctttata ggctgtatca attcgttcct
     7021 tagtcgcttc atctaactgg tcacgatgac tgccatcctc tataaaatct tccaaaatat
     7081 aagtttggta atgatacaag ggatatcctt ctggtgaaaa ggttcccttt ggtgttcgat
     7141 tgacccaagt aggatgagct ttagctgttc cgatagttgt ttttccatcc ttcttcttga
     7201 tggtgacatc catgagaaca ccacgttcag tccacttagc attctcttca tctcccatag
     7261 attcaattcg ttgattggaa atgaagttcc ccatttaata gacaatgagc tttttatctc
     7321 catctttttc caccgtttca gatggttcaa caacgtgagg atgccctcca aagataatat
     7381 ccgctcccca atcaatcatc ttgtgataaa gagctttttg ttcttcagtt ggttccaatc
     7441 gatactcaac acccatctga ggcatgataa tggtgatatc tgcttccttc tctgcccgtt
     7501 caacttcagc ctttatctta tcttcgttta aatctgaaag ataacgatta tagtcttcct
     7561 gagaaatata ctgctcaatt ccattgaaac cataggaata ggctaacaat gcaaccttga
     7621 tagcattcac ttccttaatg accagcggag cctgagcacg tggttcgtgc gtataaactc
     7681 cgattggagt gattccagct ttctcaataa tatcggccat tgaaataact ccctcaattt
     7741 gcgaatccaa aatatgatta tgagctaaat ccagcacatg ataacctgcc tccttaatag
     7801 catccataac ttcaacagga gcattaaaga gaagataacc cgctaaataa tgatccttat
     7861 taatggttcc ttcaaaatca ccaatagcta aatctgcttg cttgagccaa ggagtcacat
     7921 actcaaaatt ttcatgaaag tcataggtac cgtcttcttt tttagctgaa aagaaaagtc
     7981 catcgtggta gagcaaatcc ccgtgggcca taattctggc agttttttcc tcttcctact
     8041 cctgagactt ttgcttagtc ccctcttgag aaatagtatc tcgtttttga ctagtcaagg
     8101 gattccttgg aagctttcaa acaacaagac caagcccatt gataaaccaa ctgctagcaa
     8161 gagtatcgcc acaaatccct tattgctcca cttgcgataa ctcctaaaaa gtttaccaag
     8221 cccttcataa aacgaaaagc tgaaccaccc tgatttcgat tttgtcttct ttgtatcttc
     8281 gttctcccta ctttcttatg caagcctttt ctttttatta tatcacagat aagtatttct
     8341 ttcacaattg aattgaactt cctatctatt ttctataaat cctaaatgcc ata
//
LOCUS       DBERYS010000065         8205 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.065, whole genome shotgun sequence.
ACCESSION   DBERYS010000065 DBERYS010000000
VERSION     DBERYS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.065"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(173..1588)
                     /locus_tag="ACL8LA_001892"
     CDS             complement(173..1588)
                     /locus_tag="ACL8LA_001892"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS1813597.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1601..3439)
                     /locus_tag="ACL8LA_001893"
     CDS             complement(1601..3439)
                     /locus_tag="ACL8LA_001893"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGS1813598.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3457..4296)
                     /gene="licT"
                     /locus_tag="ACL8LA_001894"
     CDS             complement(3457..4296)
                     /gene="licT"
                     /locus_tag="ACL8LA_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGS1813599.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4595..7153)
                     /locus_tag="ACL8LA_001895"
     CDS             complement(4595..7153)
                     /locus_tag="ACL8LA_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS1813600.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            7701..8205
                     /locus_tag="ACL8LA_001896"
                     /pseudo
     CDS             7701..8205
                     /locus_tag="ACL8LA_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2711 a         1696 c         1156 g         2642 t
ORIGIN      
        1 ccaatctatc ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta
       61 atccccctat aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata
      121 tttaatgaga tgctgcctta accgtgataa tatctaatca tcaataaaca aactatttga
      181 ataaggattc tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat
      241 atcgattgaa tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct
      301 tagaaagctg tgcagtggac atagaaacgc agtcaataca tccccaagac gtatagccca
      361 taatttccac accatcttgt agagcttcag ctacttgcaa taaatgttct ttcatatact
      421 gaattctata atcatcttgg actgttaagt tattaaattc atcttttatt agttgatctt
      481 tagcacctaa tccgttttcc actataaata atggaatttg ataacggtca taatatctat
      541 ttaaaattat acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat
      601 aaggatttac taaaccacca ataatattcc cttctcctga attatactgt gttggaagag
      661 cagattgagt cacactcatg taatagctaa aggataaaaa atctacagta taatttttta
      721 ataactctgc atcttcagct gcaaactcta tgttaatgtc attttcctta aaatatcttt
      781 ttgcataatt cggataataa cctctaacat gcacatctga aaatagataa tttagattct
      841 catactcatg agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag
      901 ctaataccat acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca
      961 aactcgaggc gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct
     1021 ccttaggtat atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt
     1081 taaatgtaag ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt
     1141 tttcataaaa atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata
     1201 atggagtctc atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat
     1261 caaacaattc atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg
     1321 gaaaaattct actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca
     1381 aggatatatc ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt
     1441 ctgtaggatt ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata
     1501 agcccttacc atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc
     1561 aaagaaaatc atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt
     1621 ccttagaatg ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg
     1681 tattcgttac aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt
     1741 ccatttcaaa aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa
     1801 aaccttgacc ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct
     1861 catcactctt caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa
     1921 atggtgcata aaccttacct tcacttggaa taatcgctac tccgtctcca attagtttat
     1981 ctgaaaatgt tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa
     2041 atatcatttt tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt
     2101 catcgattcc aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa
     2161 ttaaagcatt tacaatattt gatggcacat cagaataaat aaattgaggc aacgctatca
     2221 aagatgggac agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta
     2281 atccaccacc aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata
     2341 atgcaggttc ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt
     2401 ttgtattatt atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt
     2461 ttgaccctaa cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa
     2521 aaataggtgc aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa
     2581 gaatagctaa tgtaagccat ccagctacac catacatttg cccaactaga tttgataatc
     2641 cttcaccaac aattactcca ataggcccga ctacaactaa ggcaatacag cttgatacta
     2701 ataatactag cgtaggttgc aaaaaactct tagtaacagc tggtgttaat ttagcaatca
     2761 ttttttcaat atatttcatc aaccaaacca taataagaat tggaacgact gacgaaccat
     2821 aactagctgg tgtcacaggt acaccaaata aactaagagg attccctgat tgcaccattt
     2881 gaacaaaatt tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt
     2941 ttaatttttg tgatgcagaa taagctaata acagcggtaa gaaataatat ggagcatccc
     3001 caaaaaatgt caaaaaagca atagtctgag aatctgattg caatatacca agcattggta
     3061 aaatgattac caagactttc aacatacctc cccctaacat tgctggaatg attggagtca
     3121 tggaaccagc gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa
     3181 ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat
     3241 taccaagtat aatttgatat tgtccattct ttttcataat acctattaca cctggtatct
     3301 tcttcacatc atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta
     3361 cacaatgggt aactctattg acattttttt cacctccaat tacatcgagg attttttgta
     3421 ccttatcttt ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg
     3481 acgaatatga gccatcaaat aaactaattc actagaagtc agcaaataat tgtactcagt
     3541 ttgtataaac attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat
     3601 taatgctaac aagtcttcat catcatcgtc acaataagtt gtattttcaa ccaagcgatg
     3661 ggcaaataat tttatatgag ttatgaatct ataataatca atagaatctt ggttaaactc
     3721 agtacaatag aaatcttgaa tgattttctc aattcccata attatttcag tgattttata
     3781 cgactcttga aaattatttt ctaaattagc attaacaaaa tgtagcgcta tgaatgcagc
     3841 ttcgtcaatt ggaagttcaa tacctaactc tttttttatt atttgtaaag cccttttccc
     3901 aagctcaaat tcttctttat aatatttcga gatttcccag cgtaggggat tcgaaataat
     3961 aatcccttct ttataacgtt ctatgctcga agaaatatga tccgttaaat taatatagat
     4021 aatttcatca aaattattcc ccaatgttat tttacctaga ttaattattt tttcggtgat
     4081 gtacaccaac tccaaaggaa cgttaataaa catctctgta aaacgggtca tattatctct
     4141 atttttgaga aaaaaacttt tctcaatatc aattggattt actttatctc caatttttct
     4201 tccaaaagct attccccttc caaaaagaag aatatcgata tcttttttac tttgcgcaat
     4261 tacggcattg tggtttagta ttcgttttat gatcataaaa tttccctttt aaaataaaag
     4321 gaacctactt aaaaacatca agaaacttct tccgatgtga ttaaataggt tcctgctcac
     4381 ttgtattagt gataacatcc ttttatttca atttttaatg gtgacatgaa gcttaaataa
     4441 ttaataactc tgtaaccatt ttcattttaa gatatttcaa tctatttgtc aagtacttgt
     4501 agaaattttt ttattttgtg tgcttcgaat gtgataatgt caacagtctc attaaaaaat
     4561 gaaaatcata catagaaatt tttaaaaatt ttctctatcg taaacctgcc actagttcag
     4621 aaactatact agctgattcc aaacgtaata tggtcatctc atctaccttc cctaataatg
     4681 gcattgcacc ataccaaaca atagtcttat tcaccaaaag aataggcgtt actatttttg
     4741 attgagtaaa agacacttta attttctggc tcttcaactg gtctagccat gctttatttg
     4801 caaccgtctc tggtatacat atctcaattt gactaatcct aaattctttt agaaaattca
     4861 tcagttttgt ctggtgcaca taaggtaaaa taagcagaca ttcctgtctt tcccctgcta
     4921 agtcctctct caacaccttc tcatatctac tatcaacata aacgaattgt ttctcctcac
     4981 cctcgatgac acgataatcc atctttcgat aagctacttg tcgtttctga aacatctttt
     5041 ctaaataagg aacatgaata tccacataat cgaaaatacg caccaaagac ttatccttgt
     5101 agtttctatg aatccgacct gcatactgaa ttaaattatt tttccaagaa aagggtgctg
     5161 ccaagataag cgtgtccaac tgaggtaagt caaaaccttc gccaatgtat tttccagtag
     5221 acaacaaaac aaacccttta tctaactgtt ctaacgtctc cagtaaactc gttctttctc
     5281 ggactttggt ttttccgcta ataatgtaac agccatcaac ctctttctct ttcaataact
     5341 tttcaaagac atctatctgt tgaattcgat taactaaaac caagatattc cgtccttctg
     5401 ccacttgggc tagaatatcc ttgagaatca actgattcct cgctgagtct gtagcaatcc
     5461 aatcactgag ctgtataaaa ttacttgctt tggtcttttc aatctccaaa tgaccaaaag
     5521 aagtgaatct taattgcaat tgccgtttaa aatccgtttc cctcttatca gcagtatgga
     5581 gtatctcacc aattctctga aaaacaatag gctcatgacc attcttacgc tcaggcgtag
     5641 ccgtcaaacc gtaaagatac ttccctctaa actgagcaac aactttttca aacatcaagg
     5701 cagagacatg atgacactca tccacaatca tcatctcata ctcatccaaa agactttgac
     5761 tattttccaa cttaaataga gattgaatca taacgacatc aaccagttta ctcagccatt
     5821 tcttagtccc accgtactgt ccaacatagc cgattacctt ttcacgacct gatgccgtat
     5881 aacggatagc ctcctcctct tcgaaagtca aaaagcagtt taagcgatct agccattggt
     5941 ctaagagttg cttattatgg actagaataa ttgtttttgt tttccgttca gagataagag
     6001 cagcacctaa aacggtcttc ccaaaaccag tctccgcatg aagtaaacca ttttctttag
     6061 aagtcatatc tgacagggct aactcttgct caaaagtgag ttctccctta aattccacgc
     6121 taatagacct ttgtaccttt ctcctatctt ccacagatac ctgcttaaat ttatcttgca
     6181 atggatacag caaacctctt ggcaaccata aataatgatc ggattctcca aataaataca
     6241 ttctctcagg aatttgatag gttggctgtc gcatagcctg ctttaaataa aattcgggat
     6301 tagaaaatga agccatattt ttcaagaaaa acagtgtctt gggtgtcaca gacgattttt
     6361 caagttggat catattggat aagacgatct tcaactcctt gtccaattct tgcttgccta
     6421 actcctcttg gattaacagt gccactttag cagttgaaat cttctgaatt tcttgtagat
     6481 acctccattg atcttcataa ggctgaaact gttcatccac aaagaccgtt cgcccttgat
     6541 gataagcttc tccttgaaaa ggaagggaaa ttaaattatc aaatccacct ttaggaagaa
     6601 catcctgatt tggaaacatg cgatcaaaag aatcaaagga cagttgcata ctttcctgca
     6661 tagccaattc taatagtttc tttccaaaca ggcgagcctt tcgactcaga atcgcttcct
     6721 caaagaagaa ccaaatatgg agtccgtaac ccgaacgaga aatctctaaa tgggcttcca
     6781 tctggcgttc cctggctatt cttcgaatgg ttaaaccagc ttctttccaa tctccttcat
     6841 ccaaatccag taccaaaaaa taacagctat catctaagtg cataggaaag ataccgatag
     6901 ctgcctcccc acgaaaatga gatttcaaaa cggagttcgt caatgtctgg aaactccgtt
     6961 tttcaggtgg caactgcttc caaccataat cataggaagg aaaatactga attttccctt
     7021 gttcattgat aaaattctta gcataaacat cataacggcc agcaaacaca gaggtaaaca
     7081 attgtaattt ttcttgcgtc gtcaagcgac tactttgaag ttggaacatc tcctcgaact
     7141 gagagcgttc cataacaaac tgactcttgt ctttaagacg tttatagtgg aatgaaataa
     7201 gatatgaaca aattgattag gaaaatcaaa ttaatttata gaaatctttt agcagtcatg
     7261 gcgtcctatt ccagattcaa aatgctatac acaagaaaaa ctcactatcc ccattaaaag
     7321 aagaaaagac atcaaggact tctaccacaa ttccatccaa agacacaaaa acagccataa
     7381 gagtcacctc cttgattcct ataggctgat tataacaaga ctggctgaaa ttgtacatga
     7441 aaataaaatc ctaatagtac tcattttgta tgtgactaat attccgtctc gctccagaag
     7501 gtacgaagta aatagagttt aaacgagcag tctataaaat tcaaaaaacg catattatca
     7561 agcgacctaa aactttgggc tctataatat ttgtagtggg taaattccct atggatatta
     7621 tggagcctat tttgggtaga aaaaaagtcc cataagatct ataatgaaaa gcgacaaaac
     7681 aactcattag aaagaatcat atggaacaat tacattttat cacaaaatta ctagacatta
     7741 aagaccctat aaaattcctt accttgaaac gactggtatg cctactagaa ttctccttag
     7801 aaaacgtcgt tttaagtgct atcattgctt gaaaatgatg gtcgctgaaa cttctatcat
     7861 caagaaaaat caccaaattc ctcgtattat caaccaaaag attgcgcaaa agttaattga
     7921 aaagatttct atgaccgata ttgcccatca gctggccatt tcaacttcaa ctgtcattcg
     7981 aaagctcaat gacttccact ttgagtgtaa ttttagaaat ctgcctaaga ttatgtcttg
     8041 ggacgttgaa acagtccggg gagtgactgt ttcaatcggg agatggagat gagctttatt
     8101 gcgcaagatt ttgaaaagct cgatatcatc actgttcttg agggtagaac acaagctgtc
     8161 atccgagatc actttcttaa atatgataga gccgtccgat gtcgc
//
LOCUS       DBERYS010000066         7804 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.066, whole genome shotgun sequence.
ACCESSION   DBERYS010000066 DBERYS010000000
VERSION     DBERYS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.066"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            226..6660
                     /locus_tag="ACL8LA_001897"
     CDS             226..6660
                     /locus_tag="ACL8LA_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS1813601.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
     gene            7292..7768
                     /locus_tag="ACL8LA_001898"
     CDS             7292..7768
                     /locus_tag="ACL8LA_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1813602.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
BASE COUNT         3042 a         1073 c         1493 g         2196 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca gttattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacactaa aactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagataatgc acactcgtct gcaaataaag gcacagaaaa tccgaaaaaa
      601 gaagatagac ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 acatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgca gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctattcgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcag gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgatcaaga ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct
     4261 aaatttaaaa atttaggaaa tggaaaagag ggtagtctaa aaaaagatac aactggggta
     4321 gaacatcatc atcaagaaaa tgaagagtct attaaagaaa aatctagttt tactattgat
     4381 agaaatattt caacaattag agactttgaa aataaagact taaagaaact cattaaaaag
     4441 aaatttagag aagttgatga ttttacaagt gaaactggta agagaataga ggaatacgat
     4501 tataaatacg atgataaggg aaatattatt gcttatgacg atggtagtgc cttacaatat
     4561 gaaactgaaa aacttgacga aataaaatca aaaatttatg gcgttctaag cccatctaaa
     4621 gatggacact ttgaaattct tggaaagata agcaatgttt ctaaaaatgc caaggtatat
     4681 tatggaaata gctataaatc gatagaaatc aaagcgacta agtatgactc acattcaaaa
     4741 acgatgacat ttgatttata cgctaatatt aatgatattg tggatggatt agcttttgct
     4801 ggagatatgc ggttttttgt taaagatgat aatcgaataa aagctgaaac taaaattaga
     4861 atgcctgaaa aaaataagga aactaaagca gaatatccct atgtatcaag ttatgggaat
     4921 gtaatcgaat taggagaagg agatctttca aagaacaaac cagacaattt aactaaaatg
     4981 gaatctggta aaatctattc tgattcagaa aaacaacaat atctgttaaa ggataacatc
     5041 attctaagaa aaggctatgc actaaaagtg actacctata atcctggaaa aacggatatg
     5101 ttagaaggaa atggagtcta tagcaaggaa gatatagcaa aaatacaaaa ggccaatcct
     5161 aatctaagag tcctttcaga aacaacaatt tatgctgata gtagaaatgt tgaagatgga
     5221 agaagtaccc aagctgtatt aatgtcggct ttggacggct ttaacattat aaggtatcaa
     5281 gtgtttacat ttaaaatgaa cgataaaggg gaagctatcg ataaagacgg aaatcttgtg
     5341 acagattctt ctaaacttgt attatttggt aaggatgata aagaatacac tggagaggat
     5401 aagtccaatg tagaagctat aaaagaagat ggctccatgt tatttattga taccaaacca
     5461 gtaaaccttt caatggataa gaactacttt aatccatcta aatctaataa aatttatgta
     5521 cgaaatccag aattttattt aagaggtaag atttctgata agggtggttt taactgggaa
     5581 ttgagagtta atgaatcggt tgtagataat tatttaatct acggagattt acacattgat
     5641 aacactagag attttaatat taagctgaat gttaaagacg gtgacatcat ggactgggga
     5701 atgaaagact ataaagcaaa cggatttcca gataaggtaa cagatatgga tggaaatgtt
     5761 tatcttcaaa ctggctatag cgatttgaat gctaaagcag ttggagtcca ctatcagttt
     5821 ttatatgata atgttaaacc cgaagtaaac attgatccta agggaaatac tagtatcgaa
     5881 tatgctgatg gaaaatctgt agtctttaac atcaatgata aaagaaataa tggattcgat
     5941 ggtgagattc aagaacaaca tatttatgta aatggaaaag aatatacatc atttgatgat
     6001 attaaacaaa taacagacaa gacactaaac attaagattg ttgtaaaaga ttttgcaaga
     6061 aatacaaccg taaaagaatt cattttaaat aaagatacgg gagaggtaag tgaattaaaa
     6121 cctcataggg taactgtgac cattcaaaat ggaaaagaaa tgagttcaac gatagtgtcg
     6181 gaagaagatt ttattttacc tgtttataag ggtgaattag aaaaaggata ccaatttgat
     6241 ggttgggaaa tttctggttt cgaaggtaaa aaagacgctg gctatgttat taatctatca
     6301 aaagatacct ttataaaacc tgtattcaag aaaatagagg agaaaaagga ggaagaaaat
     6361 aaacctactt ttgatgtatc gaaaaagaaa gataacccac aagtaaacca tagtcaatta
     6421 aatgaaagtc acagaaaaga ggatttacaa agagaagatc attcacaaaa atctgattca
     6481 actaaggatg ttacagctac agttcttgat aaaaacaata tcagtagtaa atcaactact
     6541 aacaatccta ataagttgcc aaaaactgga acagcaagcg gagcccagac actattagct
     6601 gccggaataa tgtttatagt aggaattttt cttggattga agaaaaaaaa tcaagattaa
     6661 gacaaaagct atagaaaaaa atggtttatg tactgagatt agatagtgag gtgatgacat
     6721 agttttgtga aaacggccat ttataactca attatttagt ttacttcact tcactattga
     6781 tactatttgg agttattaac ggacttagtt tagataacta atgaattgat tgaaagggtt
     6841 agtattgaca atattggtca tattgattag aaaatagagt ctatcaaaat ttaaaggcta
     6901 atagaggtga tgagacaatt tcaaggaatt aagtagcgtt tggttgctta attccttttt
     6961 atttggaagg aagtttttcg taaccgccca ataacgaagt ctattggacg gttacgatat
     7021 gatcactgac ttcgtcagtc ttatctacaa cctcaaaact gtgttttgag caacctgcgg
     7081 ctagcttcct agtttactct ttgattttca ttgagtatta gaacagaaaa aatgcttgga
     7141 gtatttgttt gtgtgtttat ttttatataa caaactataa acaaaacaaa aatataaaaa
     7201 aagagacaaa aaagaacaaa aagtaattga caacggtttc atatagtgtt atacttatgg
     7261 cataaagtta atggaaagaa gggaaaaact tatggtatta gattatttct ttgacaaaaa
     7321 ccttgtgttt tgcttagaag cggataatca agaacatctc tttgatcagg ttgcaacttt
     7381 attggaagaa cgagaaattg taactccaac ttatcgtgaa gccttgatca cgcgtgaaaa
     7441 gtcatttcca actggtttag atatggaatt tctaggaaag gacttgccaa atgtagcgat
     7501 tcctcacaca gatattgttc ataatctagc tgagaaagtg gtggttgttc gattagaaaa
     7561 accagtaact tttcacaata tgattgcgcc aggtaaggaa gtagaagtat ctctactctt
     7621 ctttatcatt aacaattcaa gttcaagtca aacgaacatt ctggctcagt tgatggactt
     7681 tttcacagga aatggtcatc ttgaagacct atcaaaaatt tctgaaccag aaaaacttta
     7741 tgcttacatt gctgaagcaa ccgcttaatc ttgtctatta aaaaataaaa tcggaggaaa
     7801 tcca
//
LOCUS       DBERYS010000067         7584 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.067, whole genome shotgun sequence.
ACCESSION   DBERYS010000067 DBERYS010000000
VERSION     DBERYS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.067"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..107
                     /locus_tag="ACL8LA_001899"
     CDS             <1..107
                     /locus_tag="ACL8LA_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1813603.1"
                     /translation="NNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            103..777
                     /locus_tag="ACL8LA_001900"
     CDS             103..777
                     /locus_tag="ACL8LA_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGS1813604.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGVVIGVASATTNIWIIF
                     LSGFTAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GEFKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSDYRIPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLL
                     EQLFSI"
     gene            complement(819..2807)
                     /locus_tag="ACL8LA_001901"
     CDS             complement(819..2807)
                     /locus_tag="ACL8LA_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813605.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2809..3567)
                     /locus_tag="ACL8LA_001902"
     CDS             complement(2809..3567)
                     /locus_tag="ACL8LA_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813606.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3995..4186
                     /locus_tag="ACL8LA_001903"
     CDS             3995..4186
                     /locus_tag="ACL8LA_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813607.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4540..4812)
                     /locus_tag="ACL8LA_001904"
     CDS             complement(4540..4812)
                     /locus_tag="ACL8LA_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS1813608.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(4871..5320)
                     /locus_tag="ACL8LA_001905"
     CDS             complement(4871..5320)
                     /locus_tag="ACL8LA_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGS1813609.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            complement(5307..7436)
                     /locus_tag="ACL8LA_001906"
     CDS             complement(5307..7436)
                     /locus_tag="ACL8LA_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGS1813610.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2263 a         1597 c         1481 g         2243 t
ORIGIN      
        1 ttaacaacta tcctaaaaaa tgcttggact acaagtcacc cagagaagac ttctggatgg
       61 ctaacttgaa cttgaaattt agcgtcagaa acaaatcacg aaattgattc aaattttgca
      121 ggtcgtttaa atatcctgcg tgcgggtgtt cttgatgcta acgatggaat tatttccatt
      181 gctggtgtgg ttatcggagt tgccagtgcc acgaccaata tctggattat ctttttatca
      241 ggttttacgg ctatcttagc tggtgccttt tcaatggctg gtggagaata tgtatccgtt
      301 tcaactccaa aagataccga ggaagctgcc gtttcgcgag aaaaactctt gctagaccaa
      361 gatagggaac tagccaaaaa atccctctat gctgcttata tccaaaatgg agaattcaaa
      421 acttctgccc aactcttgac caataagatc tttcttaaaa atccactcaa ggctctggta
      481 gaggaaaaat atgggattga gtatgaagaa tttaccaatc cttggcacgc tgccatttct
      541 agcttcgttg cctttttcct tagaagtttg cctccaatgc tgtcagtgac catttttcca
      601 agtgattacc gcatccctgc taccgtcctt attgtcggtg tggcccttct tctcactggt
      661 tacacaagtg ctagacttgg aaaagcccca accaaaacag ctatgattcg gaaccttgct
      721 attggtctct tgaccatggg agttaccttc ctgctcgaac aacttttcag catttagaat
      781 acaagaaata cctcgatttt gaagtcgagg tatctttttt acatttgcac aatcttgcga
      841 taacttcttg aagtaatcat gaaaatcagc acataggcga tgaggaagat agcgcagata
      901 gacaaggtca caatcaacat catagtcgta tccagtacac caatcacttt taaaatcagg
      961 ctaagcatat ggtaggcaaa ggcgagatgt atgaaggcaa agagcaaagg aaggaagaaa
     1021 acagttaaaa cctgtttgtt gatggtttgc ttgatttgct tttggtccaa accgactttc
     1081 tgcaagataa taaagcgttc acggtcttcg tagccttcag aaatttgttt gtagtagatg
     1141 accagaacag ttccgaccat aaagataatg gataggaaaa taccgataaa gaagacacca
     1201 ccaaagaggg cactcatctg agaactagca tctgctagat tgctaccata aacatagcta
     1261 ccttctgtgt ctaattgagc attaaattga ttgaggtagt tttcatactc ctcagcgacc
     1321 ttgagttgtt cttcttcgct ggcatctaca ttcataccac cgtaaaactg attatagata
     1381 gccgaatctg ggaattgatc caaaaaggct tgtaaatcag gaacaacaag gtaattgtaa
     1441 tcagtagtca agatattaaa cttatttgga acatggttga caataaaatc tttagtaaat
     1501 tcttctttga cagaaaattg atgatcattt agagttagag ctttctgtcc tttcagtccg
     1561 tcatttttgg caaagagacc gacctcattt cctgatagag acagtttttg accagtcata
     1621 ttttcataat ctttttggtc aaataccatg aaaactgttt ttggttggac acggttttgt
     1681 cctttttcaa aaatagttaa cttggttcct tcttgattcg caacagcaaa gtaagtgtaa
     1741 cgaaatactt ctttttcttt aatcttataa cctttgtcac ttgcaaactg gctcaagagt
     1801 ttgtccaagt cttctttttc aacattttgc cctgaaaccc caaaatcatg aggatttaga
     1861 acttttttaa agctttctga ggaattgaaa atgcttgtcg ctgctgacat ggttaccaaa
     1921 accattgttg acaagatagc gatggtggct agtccaactg catttttctt catacggaaa
     1981 atcaagttag aaacagatat gaggttattt ggttggtaat agtatttctt atttttcttt
     2041 aagatttgga ggaaaacggt aatccctgca ttaaacaaga gataagtccc aaagataacc
     2101 agtaaaacag ctatgaagaa ggttgttaag gctgtaagag gatcttttac cgtaagggca
     2161 agataatagc caattcctaa acttatggaa ccaagaatgg tttggagagg aaggaagcga
     2221 ccttttttct ctccactagc tttctcacga gagagctgga gggcattcat acgggcgatt
     2281 cgaagggcat tcaggaacat gaggcctagg aaaatcaaac cgaagacaac aagcactgta
     2341 atgacaactt tcgtctggaa ggtagcaacc agctcaacct tcaatttcat tagtttgagc
     2401 aggaaagcga aaattaactt gtcaaacaag gctccaatac cgatacccgc tccaacagtt
     2461 agaatcccaa ataccactaa ctccttaaag gtcatactga taagatgacg cttctccaag
     2521 cccaacatgc cataaattcc tagttccttg gaacggttct tcatgacaaa actattggca
     2581 tagagaacga taatggctga cgcaagggtg acgacaaaca taccaaatcc aagtgtagcc
     2641 tgaatggttg ttcctccacg gatttccgca atcttaggat tgaaggttag agagtaaaag
     2701 agataggtga cagtgactgc caagagaaca gccagcgcaa aaggatagta gagtttgcgg
     2761 tttttaatca agttcgatac cgctaactta ttggttaatc gaaacatact aattcacctc
     2821 gcttgccatg acagtcaagg tatcagagat ttcttggaac atctgacgtt ctgtcttctc
     2881 tccacggtag atttgattgt aaagaatgcc gtctttgata aagagtacac gtttagctct
     2941 gctagctgct gctgttgagt gggttaccat gagaatggtt tggccgcgct cattgatttc
     3001 atcaaagaca tcaagtaagg ctgcagatga cttggagtca agagctcctg tcggttcgtc
     3061 cgcaaggaga atttcaggtt ctgtgatgat ggcgcgggct actgctacac gctgtttctg
     3121 accaccagaa atctcgtaag ggtacttctc ttgtaattgg tttattccca gattctcagc
     3181 tgtcaccacc aatttcttca tcatctctgt aataggtctt cttgacaaga caagcggaag
     3241 caagatattg tcctttacag atagagtatc tagcaagtta aagtcttgga agacaaagcc
     3301 caacttttca cgacggaagc tagaggcctg tgaattttta atggttgcgg tgtcagttcc
     3361 attcaagtaa acctgaccac gacttggttt atccagcata gctagaatat tgagaagagt
     3421 tgatttacca gaaccagact cacccatgat ggcaacgtag tcaccctttt cgacggtaaa
     3481 gtgaatatcc ttgagggctt ctacttggtt gccctgaaaa cgtgttttat aaattttttg
     3541 aacgtgtttt acatctaaaa gtgtcatgag aatctccttt cttaatatcc catcaaatga
     3601 aatgtagctt gcatcatagt gcatcccagc tgaacgcgct caatttcttt gtgacttggt
     3661 tttctagttt tcttatgtaa gatttgtttc atgatgttac tcctttgttc tttatgagtc
     3721 tagtttacat caaaaaaaga ccgcttgcca taacctaacc ttacaaaaga gactttaatc
     3781 ttacattttt gtaagattgg gtgaaatata agcaaaatta cttagaaaat gatttgattt
     3841 cctattttta aagttgtaaa atataagtgt gaaaaggctt actttgaaaa ggaggaagcg
     3901 ggaactaaca ttttagtaag gttacctaga taacatacca ttcaatttaa cacttgcaag
     3961 tcgttcaacc tataaggaaa gaaggtgcgc tttaatgaaa cacaaagaac atatactcat
     4021 cggacttctc tatctgctct ctccattcat cggtcagctc ttagttgaac acacacactt
     4081 tatcggtaca gaatttaccg gcactgctta tgttatttgc tggttatcag ttgttatcag
     4141 catccaccat ttttccaaaa atgtattgtc tcagcagcaa aaatagatta aaaaactgag
     4201 tttaaattac tgcttttata ataaaatgaa ataagaacag gacaaatcga tcaggatagt
     4261 caaatcgatt tctaacaatg ttttagaagt agaggtgtcc tattctagtt ccaatccact
     4321 atagtttgtg gatgaagctg gcgaaatatt attaatacta ttcaaaaatc aaagtgcaaa
     4381 ctatgtcaac ttcacattgc cgactaaatt acagcctgtg gctggcttcc tagtttgcac
     4441 tttgattttt attgagtata aaagtaaaaa agctttaaat caagcagttt agactattga
     4501 tttaaagctt tttgcttatt ccaaatcttc tgtagaacct caccaaaacc agccttcatt
     4561 gtcgtcaatg gcttgggctt ctttgcgttt acgtgggcct gttccgtaca tttctgcttc
     4621 gatttcttcc ttggtaggca tgatagaagc taggatgata tagataatca cgccaagtcc
     4681 aaagtttgca actgtaaaaa tagcaaacaa gaaacgaact agggtaacat caaaattcca
     4741 cttgtctgac agacctgcca gaactcctga aatcatgcga ttgcgtctca ttttataaaa
     4801 tttactgttc atgatatttc ctctttctgc taggttagac atagtatatc acggggaggc
     4861 tcggctttca tcagtcctca ggctgattta tcagaagcaa tttacctcga caaagtccac
     4921 agcgatagcg tttggtatca atcctacgct tgcgctgata cctttgctgg caggattgac
     4981 aacgataaag cttgagtggt ttagagttac tattgggcaa ggatggtaca aaccgtaatc
     5041 catccactgc tttcaaaagt tccttaaaat cccgatcctt gtgttgatag cccttccctt
     5101 ggaaataaag gtgataatga cagagttcat gtcggacaat tttcctaaaa acctccatac
     5161 ccagtttctg ataaaccttg ggattaaaat ccagatgccc atctttgggg aaaaatcgcc
     5221 cacctgtcga acgtagacgc ctattccact ggacatgatg gataaaagct ctcccgaagt
     5281 cttctagtga aacctgcttg acataatcag tcagtttcat ttggagcgag gagcgacaga
     5341 ttaacttttt cacgttcagt atcaattttc ttgacccaaa cggtcaccaa atctccaact
     5401 gccaccactt gactagggtg tttgataaac ttgcgactca tatgagaaat atgaatcaag
     5461 ccgtcctcgt gaatcccgat atcaacgaag gcaccgaagt caacaacgtt acgcacaaca
     5521 ccttctagct tctgacctac cactaggtct ttgatatcta ggacatcttg gcgaagcaca
     5581 ggtgcgtcaa aggaatcacg gaaatctcga cctggtttga gaagatctgc aatgatatct
     5641 ttaagggttt ctggaccgag gtctagctct tgagccattt ccttgactga aagcgacttg
     5701 agtttgcttt gggcctcttc attcagatcc ttaatgtcca agcatttgaa gagttcctta
     5761 acggcagcgt aattttctgg gtgaactcct gtattatcaa ggatattgct actttcaggg
     5821 atacggagga aaccagcagc ctgttcaaaa gccttggccc ccaaacgagg aactttcttg
     5881 atttgggagc gtgaagtgat ttttccctct tcctcacggt atttgacgat attttcagaa
     5941 atggttttat tgagtccagc tacgtgtgaa agaagagctg ggctagctgt attgacattg
     6001 acaccaactt ggttaaccac tgtatcgaca acaaagtcca gactctcaga tagtttcttc
     6061 tgactgacat cgtgttggta ttgaccgaca ccaattgact taggatcgat tttgaccaat
     6121 tccgcaagag gatcttgcaa acgacgggcg atagaaatgg cagagcgttt ttcaacggtc
     6181 aagtctggaa actcctgacg agcaagttcg ctggcagaat agacagaagc accactttca
     6241 ttaacgataa catagctgac ttcagggaaa tctttcagaa cttccgctac aaaagcttca
     6301 ctttcacgac tggccgttcc atttccaatg gcaataatct ctacaccgta ttgaccaatt
     6361 aaatctgcta aatctttctt ggcttcttcg atttgacgag ctgatgctgg tttaacagga
     6421 taaataacct gagttgtcag catttttcct gttgcatcca cgacagctag cttggcacct
     6481 gtacgaaagg ctgggtcaaa cccaagaacc acgcgccctt tcagtggagc aaccaagagg
     6541 agattgcgca gattgtcaga aaaaagttgg atagctccct cttcagcttt ctcagttaat
     6601 tctgtccgaa tacgacgctc aatagcaggc aagacctttt tcttaacgga ttgctgaaca
     6661 acttcatcaa tataagcatt tttcaccttg aaacgagtag caaagaaggc aagaatacgg
     6721 tccgtcgcat gttcaaaacc gatcttcaag acaccaagtt tctccccacg attgagagcc
     6781 aaggtacgat agccttgcat agttccaact gtctctgaaa aatcataata aatctgaaaa
     6841 acctgctttt catcaagact ttcatccttg gcttgagaag tgagtttaga gtgtctcagc
     6901 acttcctgat aagtcataga acgcaaggtc acatcttccg ataaggcttc gaccaaaata
     6961 tcaactgcac cggtcaaggc ttccttgcca gtcgcaaatc cttcacagac gaacttttca
     7021 gcttctttct ctaagtcaac tatattctgc aaaatcaaac gagcaagagg aaagagtcca
     7081 gcttcacggg caatggttgc cttggtacga cgcttttcct tataaggaag atagagttct
     7141 tcaacgtctg ctaatttttc ggcaactaag atagcttctt ccaattcctt ggtcaactta
     7201 ccttgttctt gaatcttagc taagacagct tccttacggt cattgagatt tgtcagactt
     7261 ttatccaaat caataatagc cttaatcgcc acctcatcca gactaccagt catgtccttg
     7321 cgataacgcg cgataaaggg aatagtcgcc ccttcagctg tcaaacttag aacggtatca
     7381 atttgcttta acgtcactcc caaatcctga gagatttttt catatttttt atccataaat
     7441 ctattatacc acaagctgga aggctttctc caatcctttt catactcaat aaaaatcaaa
     7501 aagcaaacta ggaagctagc cgtaggctgc tcaaaatact gttttgaggt tgtggataag
     7561 actgacgaag tcagctcaaa acac
//
LOCUS       DBERYS010000068         7081 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.068, whole genome shotgun sequence.
ACCESSION   DBERYS010000068 DBERYS010000000
VERSION     DBERYS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.068"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(298..1989)
                     /gene="argS"
                     /locus_tag="ACL8LA_001907"
     CDS             complement(298..1989)
                     /gene="argS"
                     /locus_tag="ACL8LA_001907"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGS1813611.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2125..2571
                     /gene="argR"
                     /locus_tag="ACL8LA_001908"
     CDS             2125..2571
                     /gene="argR"
                     /locus_tag="ACL8LA_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1813612.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2622..5156
                     /gene="mutS"
                     /locus_tag="ACL8LA_001909"
     CDS             2622..5156
                     /gene="mutS"
                     /locus_tag="ACL8LA_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGS1813613.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5303..6997
                     /gene="patA"
                     /locus_tag="ACL8LA_001910"
     CDS             5303..6997
                     /gene="patA"
                     /locus_tag="ACL8LA_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HGS1813614.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1926 a         1378 c         1673 g         2104 t
ORIGIN      
        1 tatttggtta ggtgaattag tgaagtgttt aggcaagtgt ctctggttac gacgtcatgg
       61 cctctaaatc gattatattt aggggtcatg actagtgaag cagttagcta gttcgcatat
      121 aagcggctag cgtctaacaa ttaggaactt tagttccaat aactttaaga ttacgacgtt
      181 ttaggacata aatcgatcat atttatgtcc taaaactagt gaagcgccta gccaaagtcc
      241 gaataggatt tggcgttagt tacttagatt gctttgcaat caagtaactt tggcgattta
      301 catcttctct ggcgcttcta ctccaagcaa gcgaagggct tctttgagaa cgactgcggt
      361 tgcgtagctg agggctagac ggctgtcgcg ttctgggctt tcatccaaga tacgtgtatg
      421 tgcatagtat ttgttaaagg attgagccag gctaattgca aatttagcaa tgatagaagg
      481 ttcaaagtta tctgccgcac ggttgataat acgtgggaag tcttgaatga gtttaatgat
      541 ttcccagctt tcagtatcat tcaagctata gttgccagct gtttctggtt tgaaatcggc
      601 tttgcgtaag atagattgga tacgagcgta ggcatattga acgtaaggtc cagtttcacc
      661 ctcgaaggat accatagctt ctaggtcgaa gtcgtatcca tttgtacggt cagttttgag
      721 gtcatagaat ttaatggctc caaccccaac agcatgtgct acttggtctt tgttttctag
      781 ttcaggattt ttagcctcga tttggacctt ggcacggcta acagcctctg caacagtagg
      841 ctctagcaag atgacattcc ctttacgagt agagagcttc ttcccttctt ttgtaaccag
      901 accaaaagga acgtgggtaa tgtcgtcact ccaatcgtag cccatctctt gcaagacagc
      961 tttgagttgt ttaaagtggg cagattgctc ttgaccaacg acgtagatag atttagcaaa
     1021 ttggtattca tttttacggt agagggctgc agccaagtca cgtgtgatat agagagttgc
     1081 accatcagac ttcttgatga gggctggatg ttcaattcca tatttctcaa gattcacaac
     1141 ttgggcacct tctgattcaa gaagtagtcc tttttcagaa agaatgtcta caactgcatc
     1201 catcttatca ttgtagaagg cttctccgtt atagctgtca aattcaacct tcaattcatt
     1261 gtaaaggcgg ttaaattcca ctaaactttc atcgcggaac cattgccaaa gagcgagagc
     1321 ttcctcatct ccattttcaa gtttacggaa ccattcgcgc gcttcttcat ccaagctagg
     1381 gtcattttca gcttcagcgt tgatgcggac atagagttta aggagttcat cgattggatg
     1441 agcttttaca gcttcttcgt cgccccattt tttgtaggca acaatcaaca tcccaaattg
     1501 tttaccccag tctcccaaat ggttgacctt gaccgtttga taaccgattt tttggaaaat
     1561 atgtgacaag ctatctccga taacagttga acgcaggtgg ccaatagaaa atggtttagc
     1621 gatattcgga ctagacatgt cgataacaac attttcttgt ttgccaatat tttggtcagc
     1681 atagtgttct ttttcagtgg taacagcttg caatacttga gcagaaatgg cagatttatc
     1741 aaggaaaaag ttaacgtaag gtcctgttgc gacaactttt tcaaaggctt ggctgttcat
     1801 tttttcagcc agttcagccg caatcatttg tggtgcttta cgttcgactt tggcaagaga
     1861 aaaagcaggg aaagcaatgt ctcccatttc tgagttttta ggggtttcca gtaactttaa
     1921 aatagcctct tggtccaggc tatcaatgat gctagataat tcgctagcaa tcaattcttt
     1981 tgtattcatt aagagctcct ttttggactt ttctactatt ttatcacaat tttaaagaaa
     2041 gaagaaaaaa tttttgaaat ctcctgtttt tttggtataa tatggttata aatatagtta
     2101 taaatatgca cgcaagagga ttttatgaga aaaagagatc gtcatcagtt aataaaaaaa
     2161 atgattactg aggagaaatt aagtacacaa aaagaaattc aagatcggtt ggaggcgcac
     2221 aatgtttgtg tgacgcagac aaccttgtct cgtgatttgc gcgaaatcgg cttgaccaag
     2281 gtcaagaaaa atgatatggt gtattatgta ctagtaaatg agacagaaaa gattgatttg
     2341 gtggaatttt tgtctcatca tttagaaggt gttgcaagag cagagtttac cttggtgctt
     2401 cataccaaat tgggagaagc ctctgttttg gcaaatattg tagatgtaaa caaggatgaa
     2461 tggattttag gaacagttgc tggtgccaat accttattgg ttatttgtcg agatcagcac
     2521 gttgccaaac tcatggaaga tcgtttgcta gatttgatga aagataagta aggtcttggg
     2581 agttgctctc aagacttatt tttgaaaagg agagacagaa aatggcgata gaaaagctat
     2641 cacccggcat gcaacagtat gtggatatta aaaagcaata tccagatgct tttttgctct
     2701 ttcggatggg tgatttttat gaattatttt atgaggatgc ggtcaatgct gcgcagattc
     2761 tggaaatttc cttaacgagt cgcaacaaga atgccgacaa tccgatccct atggcgggtg
     2821 ttccctatca ttctgcccaa cagtatatcg atgtcttgat tgagcagggt tataaggtgg
     2881 ctatcgcaga gcagatggaa gatcctaaac aagcagttgg ggttgttaaa cgagaggttg
     2941 ttcaggtcat tacgccaggg acagtggtcg atagcagtaa gccggacagt cagaataatt
     3001 ttttggtttc catagaccgc gaaggcaatc aatttggcct agcttatatg gatttggtga
     3061 cgggtgactt ttatgtgaca ggtcttttgg atttcacgct ggtttgtggg gaaatccgta
     3121 acctcaaggc tcgagaagtg gtgttgggtt atgacttgtc tgaggaagaa gaacaaatcc
     3181 tcagccgcca gatgaatctg gtactctctt atgaaaaaga aagctttgaa gaccttcatt
     3241 tattggattt gcgattggca acggtggagc aaacggcatc tagtaagctg ctccagtatg
     3301 ttcatcggac tcagatgagg gaattgaacc acctcaaacc tgttatccgc tacgaaatta
     3361 aggatttctt gcagatggat tatgcgacca aggctagtct ggatttggtt gagaatgctc
     3421 gctcaggtaa gaaacaaggc agtcttttct ggcttttgga tgaaaccaaa acggctatgg
     3481 ggatgcgtct cttgcgttct tggattcatc gccccttgat tgataaggaa cgaatcgtcc
     3541 aacgtcaaga agtagtgcag gtctttctcg accatttctt tgagcgtagt gacttgacag
     3601 acagtctcaa gggtgtttat gacattgagc gcttggctag tcgtgtttct tttggtaaaa
     3661 ccaatccaaa ggatctcttg cagttggcga ctaccttgtc tagtgtgcca cggattcgtg
     3721 cgattttaga agggatggag caacctactc tagcctatct catcgcacaa ctggatgcaa
     3781 tccctgagtt ggagagtttg attagcgcag cgattgctcc tgaagctcct catgtgatta
     3841 cagatggggg aattatccgg actggatttg atgagacttt agacaagtat cgttgcgttc
     3901 tcagagaagg gactagctgg attgctgaga ttgaggctaa ggagcgagaa aactctggta
     3961 tcagcacgct caagattgac tacaataaaa aggatggcta ctattttcat gtgaccaatt
     4021 cgcaactggg aaatgtgcca gcccactttt tccgcaaggc gacgctgaaa aactcagaac
     4081 gctttggaac cgaagaatta gcccgtatcg agggagatat gcttgaggcg cgtgagaagt
     4141 cagccaacct cgaatacgaa atatttatgc gcattcgtga agaggtcggc aagtacatcc
     4201 agcgtttaca agctctagcc caaggaattg cgacggttga tgtcttacag agtctggcgg
     4261 ttgtggctga aacccagcat ttgattcgac ctgagtttgg tgacgattca caaattgata
     4321 tccggaaagg gcgccatgct gtcgttgaaa aggttatggg ggctcagacc tatattccaa
     4381 atacgattca gatggcagaa gataccagta ttcaactggt tacagggcca aacatgagtg
     4441 ggaagtctac ctatatgcgt cagttagcca tgacggcggt tatggcccag ctgggttcct
     4501 atgttcctgc tgaaagcgcc catttaccga tttttgatgc gatttttacc cgtatcggag
     4561 cagcagatga cttggtttcg ggtcagtcaa cctttatggt ggagatgatg gaggccaata
     4621 atgccatttc gcatgcgacc aagaactctc tcattctctt tgatgaattg ggacgtggaa
     4681 ctgcaactta tgacgggatg gctcttgctc agtccatcat cgaatatatc catgagcaca
     4741 tcggagctaa gaccctcttt gcgacccact accatgagtt gactagtctg gagtctagtt
     4801 tacaacactt ggtcaatgtc cacgtggcaa ctttggagca ggatgggcag gtcaccttcc
     4861 ttcacaagat tgaaccggga ccagctgata aatcctacgg tatccatgtt gccaagattg
     4921 ctggcttgcc agcagacctt ttagcaaggg cggataagat tttgactcag ctagagaatc
     4981 aaggaacaga gagtcctcct cccatgagac aaactagtgc tgtcactgaa cagatttcac
     5041 tctttgatag ggcagaagag catcctatcc tagcagaatt agctaaactg gatgtgtata
     5101 atatgacacc tatgcaggtt atgaatgtct tagtagagtt aaaacagaaa ctataaaacc
     5161 aagactcact agttaatcta gctgtatcaa ggagacttct ttgacaattc tccacttttt
     5221 tgctagaata acatcacaca aacagaatga aaaggagctg acgcattgtc gctccctttt
     5281 gtctattttt taaggagaaa gtatgctgat tcagaaaata aaaacctaca agtggcaggc
     5341 cctggcttcg ctcctgatga caggcttgat ggttgctagt tcacttctgc aaccgcgtta
     5401 tctgcaggaa gtcttaggcg ccctccttgc tgggaaatat gaagctattt atagtatcgg
     5461 ggcttggttg attggtgtgg ccgtagtcgg tctagttgct ggtggactca atgttgtcct
     5521 cgcagcctat attgcccaag gagtttcatc cgaccttcgg gaggatgcct tccgtaaaat
     5581 tcaaaccttt tcttatgctg atattgaaca atttaatgcg ggaaatctag tcgttcgaat
     5641 gacaaatgat atcaaccaga ttcagaacgt tgtcatgatg accttccaga ttcttttcag
     5701 acttcccctc ttgttcatcg gttcgtttat cctagcggtt caaaccttac cttctctgtg
     5761 gtgggtgatt gttctcatgg tagtcttgat ttttggtttg actgctgtca tgatgggaat
     5821 gatggggcct cgttttgcca agtttcaaac ccttcttgag cgcatcaatg ccattgccaa
     5881 ggaaaattta cgtggcgttc gtgtggtcaa gtcctttgtc caagaaaaag agcaatttgc
     5941 taagtttaca gaggtctcag acgagcttct tggtcaaaac ctttacattg gttatgcctt
     6001 ttcagtagtg gaacccttta tgatgttggt tggttacggg gcggtcttcc tctctatttg
     6061 gctggtcgcg ggaatggttc agtcggatcc gtctgttgtt ggttccatcg cttcttttgt
     6121 taattaccta agccagatta tctttaccat tgttatggtt ggatttttgg gaaattctgt
     6181 cagccgtgcc atgatttcca tgcgtcgtat tcgagaaatt cttgacgcag agccagctat
     6241 gaccttcaag gatatcccag atgaagagtt ggttggaagc cttagctttg aaaatgtgac
     6301 ctttacctat ccaatggaca aggaaccgat gctgaaagat gtgagcttta ctattgaacc
     6361 tggtcaaatg gttggtgtag ttggagcgac tggtgcagga aagtcaacct tggctcaatt
     6421 gattccacgt ctctttgatc cacaggaagg ggctatcaaa atcggaggta aggatattcg
     6481 agaagtgagt gaaggaaccc tgcgtaaaac agtttccatc gttctccaac gtgccattct
     6541 ttttagtgga acgattgcag ataacttgag acaggggaag ggaaatgcta ctctatttga
     6601 aatggagcgc gcagccaata ttgcccaggc tagtgaattc attcatcgta tggagaaaac
     6661 ctttgaaagt ccagttgaag aacggggaac caatttctct ggtggacaaa aacaaaggat
     6721 gtcgattgcg cgtgggattg tcagcaatcc acgtattctg atttttgatg attcgacctc
     6781 agccttggat gccaaatcag agcgcttggt gcaagaagct ttgaataagg acttgaaggg
     6841 gacgacaacc attattattg ctcaaaaaat tagctcggtt gtccatgcag acaagatctt
     6901 ggttctaaat caaggacgat tgattggtca aggcacgcat gcagacttgg ttgccaacaa
     6961 tgccgtttac cgtgaaatct atgaaacaca gaaatgaaag acaaactata agaaaagtca
     7021 atagttttat ctaaactatt tcttatttca atttgatgat ttggcgatga ttttagagca
     7081 c
//
LOCUS       DBERYS010000069         6719 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.069, whole genome shotgun sequence.
ACCESSION   DBERYS010000069 DBERYS010000000
VERSION     DBERYS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.069"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(28..291)
                     /locus_tag="ACL8LA_001911"
                     /pseudo
     CDS             complement(28..291)
                     /locus_tag="ACL8LA_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            489..2255
                     /gene="patB"
                     /locus_tag="ACL8LA_001912"
     CDS             489..2255
                     /gene="patB"
                     /locus_tag="ACL8LA_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGS1813615.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2394..3083
                     /locus_tag="ACL8LA_001913"
     CDS             2394..3083
                     /locus_tag="ACL8LA_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS1813616.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3087..3266
                     /locus_tag="ACL8LA_001914"
     CDS             3087..3266
                     /locus_tag="ACL8LA_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813617.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3253..4602
                     /locus_tag="ACL8LA_001915"
     CDS             3253..4602
                     /locus_tag="ACL8LA_001915"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGS1813618.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4726..6186
                     /gene="gltX"
                     /locus_tag="ACL8LA_001916"
     CDS             4726..6186
                     /gene="gltX"
                     /locus_tag="ACL8LA_001916"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGS1813619.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            6200..6631
                     /locus_tag="ACL8LA_001917"
     CDS             6200..6631
                     /locus_tag="ACL8LA_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS1813620.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT         2111 a         1289 c         1442 g         1877 t
ORIGIN      
        1 gcatgctttg gcagcttgta tgagtaccta ccgcagatga ggggaacccc gtttgaccat
       61 tctttctgct aaatcaatct gacctgactg ataaatagaa gaatccagtc cagcgaaagc
      121 ttgtaattga gcaggattat caaaggcatg aatatttcga atctcggcta aaatgaccgc
      181 ccctaaacga cccccaatcc cagtaaccgt cgtgatgacc gagttgaact cagccatcaa
      241 gtcattgaca cattttttcg ccttgtcaat gagcctcttg taatgtttga tgttttcatt
      301 acaagagata aaacgtttat gcgttatcaa actcattacc aattgaaaca aaaagctgtg
      361 gttagatcct ttcggaaatt gtcaagcgat tggaggaaat gaactaatcc acagcggctt
      421 attccaagta taccacttgg gctttggcag tagctaactg cgctaaatat aatataagga
      481 ggagtaaaat gaagacagtt caattttttt ggcattattt taaggtctac aagttctcat
      541 ttgtagttgt catcctgatg attgttctgg cgacttttgc ccaagccctc tttccagtct
      601 tttctggaca agcggtgacg cagctagcca atttagttca agcttatcaa aatggcaatc
      661 cagaacttgt atggcaaagc ctatcaggaa tcatggtcaa tcttggcctg ctggttttgg
      721 ttctatttat ctctagtgta atatacatgt gtctcatgac gcgcgtgatt gcagaatcga
      781 ccaacgagat gcgcaaaggc ctctttggta agcttgctca gttgacggtt tctttctttg
      841 accgtcgaca agatggcgat atcctgtctc attttaccag tgatttggat aatatcctcc
      901 aagcctttaa cgaaagcttg attcaggtca tgagcaatat tgttttatac attggtctga
      961 ttcttgtcat gttttcgaga aatgtgacgc tggctctcat caccattgcc agcactccat
     1021 tggctttcct tatgctgatt ttcatcgtga aaatggcacg taaatacacc aacctccagc
     1081 agaaagaggt agggaagctc aacgcctata tggatgagag catctcaggc caaaaagccg
     1141 tgattgtgca aggaattcaa gaggatatga tggcaggatt tcttgaacaa aatgagcgcg
     1201 tgcgcaaggc aacctttaaa ggaagaatgt tctcaggaat tcttttccct gtcatgaatg
     1261 ggatgagcct gattaataca gccatcgtca tctttgctgg ttcggctgta cttttgaatg
     1321 ataagtctat tgaaacaagt acagccctag gtttgattgt tatgtttgca caattttcac
     1381 agcagtacta ccagcctatt atccaagttg cagcgagttg gggaagcctt cagttggcct
     1441 ttactggagc tgaacgaatt caggaaatgt ttgatgcaga ggaggaaatc cgacctgaaa
     1501 aggctccaac cttcactaag ttgcaagaaa gtgttgaaat cagtcatatc gatttttcat
     1561 acttgcctga taaacctatt ttgaaagatg tcagcatttc tgcccctaaa ggccagatga
     1621 cagcagttgt tgggcctaca ggttcaggaa aaacgactat tatgaacctc atcaatcgct
     1681 tttatgatgt tgatgctggt ggtatttatt ttgatggtaa agacattcgt ggctatgact
     1741 tagatagtct tagaagcaag gtgggaattg tattgcaaga ttcggtcttg tttagcggaa
     1801 cgattcgaga caatatccga tttggtgtgc cagatgctaa tcaggaaatg gttgaggtag
     1861 cagcaaaagc aacccacatt cacgactata tcgaaagttt gcctgataag tacgatactc
     1921 ttattgatga tgaccagagc atcttttcaa cagggcagaa gcaattgatt tcaatcgctc
     1981 gaaccctgat gacagatcca gaagttctca ttctcgatga agcaacttca aacgtagata
     2041 cggtgacgga aagcaagatt cagcatgcca tggaggtggt tgtagcaggt agaactagtt
     2101 tcgtcattgc ccaccgcttg aaaaccattc tcaatgcaga tcagattatt gtccttaaag
     2161 atggagaagt cattgaacgt ggtaaccacc atgaactttt gaagctaggt ggcttttatt
     2221 cagaactcta tcacaatcaa tttgttttcg aataagaaag aagttgtcct atgtgggcag
     2281 ctttttcttg tccataaaaa atgtttatca cagccttaaa aaaaacatat tagacgaaag
     2341 tcattttgag tgatatgata ggactatcgt tagtattcga aaggagaggc atcatggcta
     2401 gaacggttgt aggagttgct gcaaatctat gtcccgtaga cgcagaaggc aaaatcattc
     2461 attcatctgt atcttgtaga ttcgcagaga tcattcgtca agtcggtggt ctccctttag
     2521 tcattcctgt tggtgatgag tcagttgtac gtgattatgt ggaaatgatt gacaaactca
     2581 ttttgacagg aggccaaaat gttcatcctc agttttatgg agagaaaaag accgtcgaaa
     2641 gcgatgatta caatctggtc cgtgacgaat ttgaattggc actcttgaag gaagcgcttc
     2701 gtcagaataa accaattatg gcaatctgtc gcggtgtcca acttgtcaat gttgcctttg
     2761 gtggaaccct caatcaagaa atcgaaggtc actggcaagg actacctttc gggacatctc
     2821 actctattga aacagtagaa ggaagcgtgg tggctaagct atttgggaaa gaaagtcagg
     2881 tcaactcagt ccatcgtcaa agcattaaag atttggcacc taatttccgt gtaactgcta
     2941 ttgattcaag agaccaaact attgaagcga ttgagtctat cgacgagcac cgcattatcg
     3001 gtttacagtg gcatccagag tttctggtta atgaagaaga tggcaattta gaattatttg
     3061 agtatttatt gaatgaactg taacgattgg aacatctggt cgttctttct tttatttttt
     3121 caaaattttt agaatgtggc attttacgca aacgtttgaa ttctgataaa aattggagaa
     3181 attcgacaaa aaaacttgaa aaaaacgaag gtaagcgtta tgatagaaaa gaagaaatat
     3241 tggaggaaga acatgtcaca tattaaattt gattattcaa aagttttaga caaatttgtt
     3301 gcaccacatg aagtggaata catgcaatca caagtaacag cagcagatga attgatccgt
     3361 aaaggaactg gtgctggtag cgacttcttg ggatggttgg accttcctga aaaatacgac
     3421 cgcgaagaat ttgaccgcat cttgaaagct gctgagcaaa tcaagtcaga cagcgatgtt
     3481 ttggttgtga tcggtattgg tggatcttac cttggtgcaa aagcagcaat cgacttcttg
     3541 aatcaccatt ttgctaactt gcaaacaaaa gaagaacgca aagctccaca aatcctttac
     3601 gcaggaaact caatctcatc tacttacctt gctgacttgg tagagtatgt tgcagacaaa
     3661 gacttctcag taaacgtaat ttctaaatca ggtacaacaa ctgaaccagc gattgctttc
     3721 cgtgtcttta aagaactctt ggttaagaaa tacggtcaag aagaagctaa caaacgtatc
     3781 tatgcaacaa ctgaccgcca aaagggtgct gttaaggttg aagcagacgc taacggttgg
     3841 ggaacatttg ttgttccaga tgatatcggt ggacgcttct cagtattgac agccgttggt
     3901 ttgcttccaa tcgcagcatc aggagctgac atcaaagctc ttatggaagg tgcgaatgca
     3961 gctcgcaaag actacacttc agacaaaatc tctgaaaacg aagcttacca atacgcagtc
     4021 gttcgtaaca tcctttatcg taaaggctat gcaactgaga tcttggtaaa ctatgagcca
     4081 tcacttcaat acttctcaga atggtggaaa caattggctg gtgaatcaga aggaaaagac
     4141 caaaaaggta tctacccaac ttcagccaac ttctcaactg acttgcactc acttggtcaa
     4201 tttatccaag aaggaactcg tatcatgttt gaaacagttg tccgtgttga caaacctcgt
     4261 aaaaacgtgc ttattcctac tttggaagaa gaccttgacg gacttggtta ccttcaagga
     4321 aaagacgttg actttgtaaa caaaaaagca actgacggtg ttcttcttgc ccacacagat
     4381 ggtgatgtac caaacatgta tgtgactctt ccagagcaag acgctttcac tcttggttac
     4441 actatctact tcttcgaatt ggcaattgcc ctttcaggtt acttgaatgc tatcaaccca
     4501 tttgaccaac caggtgttga agcttataaa cgtaacatgt ttgcccttct tggaaaacca
     4561 ggatttgaag aattgagcaa agaacttaac gcacgtctat aatagaagaa aagagtggtt
     4621 tgcccactct ttttactctc tttatccata gaaattggac tcagccaaga cttgtgatat
     4681 aatatagaaa gcaaaaaggc agacgcctag ataataggag aaactatgtc aaaagatatc
     4741 cgcgtacgtt acgcaccaag tccaacagga ctactacaca tcggaaatgc ccgtacagca
     4801 ttgtttaact acttgtatgc gcgccatcat ggtggaacat ttctcatccg tatcgaagat
     4861 actgaccgta aacgccatgt cgaggatggt gaacgttcac aacttgaaaa ccttcgctgg
     4921 ttaggcatgg attgggatga aagtccagaa tcacatgaga attatcgcca gtctgagcgt
     4981 ttggacttgt atcaaaaata cattgaccaa ctattagctg aaggaaaagc ctataaatct
     5041 tacgttacag aagaagagtt ggcagctgaa cgtgaacgtc aagaagtagc tggcgaaaca
     5101 ccacgctaca tcaatgaata ccttggtatg agtgaagaag aaaaagcggc ttatatcgca
     5161 gaacgtgaag cagctggtat cataccaaca gttcgtttgg ctgtcaatga gtcaggtatc
     5221 tacaagtggc atgatatggt caaaggtgat atcgaatttg aaggtggcaa tatcggcggt
     5281 gactgggtta tccaaaagaa agacggttac ccaacttaca actttgccgt tgttatcgat
     5341 gaccacgata tgcaaatctc tcatgttatc cgtggagatg accatattgc taatacacca
     5401 aaacagctta tggtctatga agctcttggt tgggaagctc cagagtttgg tcacatgacc
     5461 ttgattatca actctgagac tggtaagaag ttatctaaac gtgatactaa tacacttcaa
     5521 tttatcgaag attaccgaaa gaaaggttac cttccagaag cagtctttaa ctttattgct
     5581 cttcttggtt ggaacccagg tggtgaagat gagattttct ctcgtgaaga actcattaaa
     5641 ctttttgatg aaaaccgcct cagcaagtca ccagcagcct ttgatcagaa aaaattagac
     5701 tggatgagca atgattatat caagaatgca gaccttgaaa ccatctttga aatggcaaaa
     5761 ccattcttag aggaagcagg ccgtttgact gacaaggctg aaaaattagt tgagctctat
     5821 aaaccacaaa tgaaatcagt agatgagatt atcccattga cagatctttt cttctcagat
     5881 ttcccagaat tgacagaagc agagcgcgaa gtcatgacgg gtgaaacagt tccaacagtt
     5941 cttgaagcat tcaaagcaaa acttgaagcg atgacagatg atgaatttgt gacagaaaat
     6001 atcttcccac aaattaaagc agtccaaaaa gaaacaggta ttaaagggaa aaatcttttc
     6061 atgcctattc gtatcgcagt ttcaggcgaa atgcatgggc cagaattacc agatacaatt
     6121 ttcttgcttg gacgtgaaaa atcaattcag catatcgaaa acattctaaa agaaatctct
     6181 aaataaagag gatacaataa tggacatctg ggaaaagatg tacgaagaag cacagaaact
     6241 atacaatcca catgaagtat cagactttgt ttatgccaat catgttgtag ctgcagtaga
     6301 agcaggagat ggacaaatat ttacaggatt ctgtatggag ggaacctgtg gtgttttcca
     6361 tctctgcgca gaacgggcga ccctcttcaa tatgtaccaa ttttcaggac aaactaaggt
     6421 taagaaagta ttagcctttc gagacaaacc accttatggt ggaagttcag ccatgccttg
     6481 cggtgcttgt agagaattcc ttttagagct taatgctgaa aataaagatg cagaattcat
     6541 gatggactat aatataagaa aaacagttaa agtcgcagaa ctaatccctt attggtgggg
     6601 agaagaacgt gcttctaagt ttaatgagtg atagaagagt cagtataatt ctggctcttt
     6661 ttactataag ttgaaaaaat aaaaaaaatt agaaaaagta gttgacaaaa tttgaaaag
//
LOCUS       DBERYS010000070         6476 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.070, whole genome shotgun sequence.
ACCESSION   DBERYS010000070 DBERYS010000000
VERSION     DBERYS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.070"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            110..310
                     /locus_tag="ACL8LA_001918"
     CDS             110..310
                     /locus_tag="ACL8LA_001918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGS1813621.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            345..1061
                     /gene="atpB"
                     /locus_tag="ACL8LA_001919"
     CDS             345..1061
                     /gene="atpB"
                     /locus_tag="ACL8LA_001919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGS1813622.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1075..1569
                     /gene="atpF"
                     /locus_tag="ACL8LA_001920"
     CDS             1075..1569
                     /gene="atpF"
                     /locus_tag="ACL8LA_001920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGS1813623.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1569..2105
                     /locus_tag="ACL8LA_001921"
     CDS             1569..2105
                     /locus_tag="ACL8LA_001921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGS1813624.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2120..3625
                     /gene="atpA"
                     /locus_tag="ACL8LA_001922"
     CDS             2120..3625
                     /gene="atpA"
                     /locus_tag="ACL8LA_001922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGS1813625.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3641..4519
                     /locus_tag="ACL8LA_001923"
     CDS             3641..4519
                     /locus_tag="ACL8LA_001923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGS1813626.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4605..6011
                     /gene="atpD"
                     /locus_tag="ACL8LA_001924"
     CDS             4605..6011
                     /gene="atpD"
                     /locus_tag="ACL8LA_001924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGS1813627.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6022..6441
                     /locus_tag="ACL8LA_001925"
     CDS             6022..6441
                     /locus_tag="ACL8LA_001925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGS1813628.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
BASE COUNT         1924 a         1213 c         1431 g         1908 t
ORIGIN      
        1 aaatggaaat aatttttatt aaacgcttcc aatagcggtt aaaagttgac aaatttaaat
       61 tttaggacta cactaagtaa gtaaatttta ttttaaagga gaatttgtta tgaatttaac
      121 atttttaggc ttatgtattg cctgtatggg cgtatctgtc ggtgaaggtt tattgatgaa
      181 tggactgttt aaatcagtag cacgccaacc agatatgctt tctgagtttc gtagtttgat
      241 gtttttaggt gttgccttta ttgaaggaac tttctttgta actcttgtct tctcatttat
      301 tatcaaataa atacatggaa cgagaagaaa agggaggatt ttagatggaa gaaagtatta
      361 atccaatcat ctctattggt cctgttatct tcaatctgac tatgttagcc atgactttgt
      421 tgattgtggg agttattttt gtctttattt attgggcaag ccgcaatatg accttgaaac
      481 ccaaaggaaa gcaaaatgta cttgagtatg tctatgactt tgttattgga tttacagaac
      541 ctaacattgg ttcgcgctac atgaaagatt actcactctt tttcctttgt ttattccttt
      601 tcatggtgat tgccaataac cttggcttaa tgacaaagct tcaaacgatc gatgggacta
      661 actggtggag ttcgccaacc gctaatttac agtatgactt aaccttatct tttcttgtca
      721 ttttgttgac acatatagaa agcgttcgtc gtcgtggatt taaaaaaagt ataaaatctt
      781 ttatgagtcc tgtttttgtc ataccgatga atatcttgga agaatttaca aacttcttat
      841 ctttggcttt gcggattttt gggaatatct ttgcaggaga ggtcatgacg agtttgttac
      901 ttcttctttc ccaccaagct atttattggt atccagtagc ctttggagct aatttggctt
      961 ggactgcatt ttctgtcttt atttcctgca tccaagctta tgtttttact cttttgacat
     1021 ctgtgtattt agggaataag attaatattg aagaggaata gaaaggagta actgatgcac
     1081 gtaacagtag gtgaattaat tggtaatttt attttaatca ctggctcttt tattcttttg
     1141 ctagtcttga ttaaaaaatt tgcatggtct aatattacag gcattttcga agaaagagct
     1201 gaaaaaattg cttcagatat tgacagagct gaagaagccc gtcaaaaagc agaagtattg
     1261 gctcaaaaac gcgaagatga attggctggt agccgtaaag aagctaagac aatcattgaa
     1321 aatgcaaagg aaacagctga gcaaagtaag gctaatatct tagcagatgc taaactagaa
     1381 gcaggacact taaaagaaaa agccaatcaa gaaattgctc aaaataaagt agaagcttta
     1441 cagagtgtta agggtgaggt cgcagatttg accatcagct tagctggtaa aatcatctca
     1501 caaaaccttg acagtcatgc ccataaagca ctcattgatc agtatatcga tcagctagga
     1561 gaagcttaat ggacaagaaa acagtaaagg taattgaaaa atacagcatg ccttttgtcc
     1621 aattggtact tgaaaaagga gaagaagacc gtatcttttc agacttgact caaatcaagc
     1681 aagttgttga aaaaacaggt ctgccttctt ttttaaaaca agtggcagta gacgagtcgg
     1741 ataaggaaaa aacaattgct tttttccaag attctgtgtc gcctttatta caaaacttta
     1801 tccaggttct ggcctacaat cacagagcaa atctttttta tgatgtgctt gtagattgct
     1861 tgaaccgact tgaaaaagaa acaaatcgat ttgaagtgac gattacgtct gctcatcctc
     1921 taactgatga acagaagact cgtttgctcc ctttgattga gaaaaaaatg tctctgaaag
     1981 taaggagtgt aaaagaacaa atcgatgaaa gtctcattgg tggttttgtc atttttgcca
     2041 atcacaagac aattgatgtg agtattaaac aacaacttaa agttgttaaa gaaaatttga
     2101 aatagaaagt ggtgttcttt tggcaattaa cgcacaagaa atcagcgctt taattaagca
     2161 acaaattgaa aatttcaaac ccaattttga tgtgactgaa acaggtgttg taacctatat
     2221 cggggacggt atcgcgcgtg ctcatggcct tgaaaatgtc atgagtggag agttgttgaa
     2281 ttttgaaaac ggctcttatg gtatggctca aaacttggag tcaacagacg ttggtattat
     2341 catcctaggt gactttacag atatccgtga aggcgataca atccgccgta cagggaaaat
     2401 catggaagtc cctgtaggtg aaagtctgat tggtcgtgtt gtggatccgc ttggtcgtcc
     2461 agttgacggt cttggagaaa tccacactga taaaactcgt ccagtagaag caccagctcc
     2521 tggtgttatg caacgtaagt ctgtttcaga accattgcaa actggtttga aagctattga
     2581 cgcccttgta ccgattggtc gtggtcaacg tgagttgatt atcggtgacc gtcagacagg
     2641 gaaaacaacc attgcgattg atacaatctt gaaccaaaaa gatcaagata tgatctgtat
     2701 ctacgtcgcg attggacaaa aagaatcaac agttcgtacg caagtagaaa cacttcgtca
     2761 gtacggtgcc ttggactaca caatcgttgt gacagcctct gcttcacaac catctccatt
     2821 gctcttccta gctccttata ctggggttgc tatggcggaa gaatttatgt atcaaggtaa
     2881 gcatgttttg attgtatacg atgatctatc aaaacaagcg gtagcttatc gtgaactatc
     2941 gctcttgctt cgtcgtcctc caggtcgtga agccttccca ggggatgttt tctatctcca
     3001 cagccgtttg cttgagcgct cagctaaagt ttctgatgaa cttggtggtg gatcaattac
     3061 agccctacca tttatcgaga cacaagcagg agatatctca gcctatatcg caaccaacgt
     3121 gatttctatc actgatggac aaatcttcct tggcgatggc ctcttcaatg caggtattcg
     3181 tccagccatc gatgcgggtt catctgtatc tcgtgtaggt ggttctgcac aaatcaaagc
     3241 catgaagaag gttgctggta cacttcgtat cgaccttgct tcataccgtg agttggaagc
     3301 ctttactaag tttggttctg acttggacgc agcaacacag gctaagttga accgtggacg
     3361 tcgtaccgtt gaggtcttga aacaacctgt tcacaaacca ttacctgttg agaaacaagt
     3421 aaccattctt tatgctttga cacatggttt cttggatact gttccagtag atgatattgt
     3481 tcgtttcgag gaagagttcc atgccttctt tgatgctcaa catccagaga ttttggaaac
     3541 cattcgtgat acaaaagact tgccagaaga agcagtcttg gatgctgcga ttacagagtt
     3601 tctcaatcaa tctagcttcc aataagaata gaggtgtcag atggcagtgt ctctaaatga
     3661 tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa atcactaatg ccatgcaaat
     3721 ggtatcggct gctaagttag gtcgctctga agaagctgct cgcaacttcc aagtttacgc
     3781 tcagaaagtg cgtaaacttt tgacagatat ccttcatggt aatggagctg gtgcttcaac
     3841 taatccgatg ttgattagcc gttctgtgaa gaagacaggc tatatcgtta tcacttcaga
     3901 ccgcggtttg gttggaggtt ataattcctc tattttgaaa gctgttatgg agttgaaaga
     3961 agaataccac ccagacggta aaggttttga aatgatctgt atcggtggga tgggagctga
     4021 tttctttaag gctcgcggta ttcaaccact ttatgaatta cgtggcttgg cagaccaacc
     4081 tagctttgat caagttcgta agattatttc aaaaactgtt gaaatgtacc aaaatgaact
     4141 ctttgatgag ctttatgttt gctacaacca ccatgtcaat acgctaacca gtcaaatgcg
     4201 tgtggaacaa atgcttccga ttgttgactt ggatccaaat gaagcggatg aagagtacag
     4261 cttgactttt gaattggaaa ccagccgaga agaaattctg gagcagttgt tgcctcagtt
     4321 tgcagaaagt atgatttacg gtgccattat cgatgccaag acagctgaga atgctgcggg
     4381 tatgacagcc atgcaaacag cgacagataa tgctaagaaa gtcatcaatg atttgacaat
     4441 tcagtataac cgtgccagac aggcggcgat tacacaagaa attacagaaa tcgtagcagg
     4501 tgctagtgcc ttagaatagg ctctagtcca gctcgtatga aaatgaactt aggacctagt
     4561 tgagctagga accgacagta tcttatatag aataggagaa ggagatgagt tcaggtaaaa
     4621 ttgctcaggt tatcggtccc gttgtagacg ttttgtttgc agcaggggaa aaacttcctg
     4681 agattaacaa tgcacttgtc gtctacaaaa atgacgaaag aaaaacaaaa atcgtccttg
     4741 aagtagcctt ggagttagga gatggtatgg ttcgtactat cgccatggaa tcaacagatg
     4801 ggttgactcg tggaatggaa gtattggaca caggtcgtcc aatctctgta ccagtaggta
     4861 aagaaacttt gggacgtgtc ttcaacgttt tgggagatac cattgatttg gaagctcctt
     4921 ttacagaaga cgcagagcgt cagccaattc ataaaaaagc tccaactttt gatgagttgt
     4981 ctacctcttc tgaaatcctt gaaacaggga tcaaggttat tgaccttctt gccccttacc
     5041 ttaaaggtgg taaagttgga cttttcggtg gtgccggagt tggtaaaacc gtcttaatcc
     5101 aagaattgat tcacaacatt gcccaagagc acggtggtat ttcagtattt actggtgttg
     5161 gggaacgtac tcgtgagggg aatgaccttt actgggaaat gaaagaatca ggcgttatcg
     5221 agaaaacagc catggtcttt ggtcagatga atgagccacc aggagcacgt atgcgtgttg
     5281 cccttactgg tttgacaatc gctgaatact tccgtgatgt ggaaggccaa gacgtgcttc
     5341 tctttatcga taatatcttc cgtttcactc aggctggttc agaagtatct gcccttttgg
     5401 gtcgtatgcc atcagccgtt ggttaccaac caacacttgc tacggaaatg ggtcaattgc
     5461 aagaacgtat cacatcaacc aagaagggtt ctgtaacctc tatccaggct atctatgtgc
     5521 cagcggatga ctatactgac ccagcgccag caacagcctt cgctcacttg gattcaacaa
     5581 caaacttgga acgtaaattg gtacaattgg gtatctaccc agccgttgac ccacttgctt
     5641 caagctcacg tgccttggca cctgaaatcg ttggagaaga gcactatgca gttgctgctg
     5701 aagtaaaacg tgtccttcaa cgttaccatg aattgcaaga tatcattgct atccttggta
     5761 tggatgagct ttctgatgaa gaaaagacct tggttgctcg cgcccgtcgt atccagttct
     5821 tcttgtcaca aaacttcaac gttgcggaac aatttactgg tcagccaggt tcttatgttc
     5881 cagttgctga aactgtacgt ggctttaagg aaatccttga tggtaaatac gaccacttgc
     5941 cagaagatgc cttccgtggt gtaggttcta tcgaagatgt gattgcaaaa gctgaaaaaa
     6001 tgggatttta agaggtgacc tatggctcag ttaactgtcc agatcgtgac accagatggt
     6061 ctcgtctatg atcaccatgc cagctatgta tcggttcgaa ctctggatgg tgagatgggg
     6121 atcttgccac gacatgaaaa tatgattgcg gttttagcag ttgatgaagt aaaggtaaaa
     6181 cgtatcgatg ataaagatca cgtgaactgg attgcagtaa acggtggcgt tattgaaatt
     6241 gccaatgata tgatcacaat cgtcgctgac tctgcagaac gtgctcgtga tatcgatatt
     6301 agccgtgcag aacgtgccaa acttcgtgca gaacgtgcaa ttgaagaagc acaagacaaa
     6361 catttgattg accaagaacg tcgtgctaag attgctttgc aacgtgccat taaccgtatt
     6421 aatgtcggaa atagactata agaaaaaatg aacttgaaaa taccaagttc attttt
//
LOCUS       DBERYS010000071         6463 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.071, whole genome shotgun sequence.
ACCESSION   DBERYS010000071 DBERYS010000000
VERSION     DBERYS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.071"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            79..1054
                     /locus_tag="ACL8LA_001926"
                     /pseudo
     CDS             79..1054
                     /locus_tag="ACL8LA_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1231..2238
                     /gene="gap"
                     /locus_tag="ACL8LA_001927"
     CDS             1231..2238
                     /gene="gap"
                     /locus_tag="ACL8LA_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGS1813629.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2306..3181)
                     /locus_tag="ACL8LA_001928"
     CDS             complement(2306..3181)
                     /locus_tag="ACL8LA_001928"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1813630.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVRWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTF
                     TTLSNTFEKELKKNG"
     gene            3268..5463
                     /gene="pbp2a"
                     /locus_tag="ACL8LA_001929"
     CDS             3268..5463
                     /gene="pbp2a"
                     /locus_tag="ACL8LA_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGS1813631.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5516..5668
                     /gene="rpmG"
                     /locus_tag="ACL8LA_001930"
     CDS             5516..5668
                     /gene="rpmG"
                     /locus_tag="ACL8LA_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1813632.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5678..5854
                     /gene="secE"
                     /locus_tag="ACL8LA_001931"
     CDS             5678..5854
                     /gene="secE"
                     /locus_tag="ACL8LA_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGS1813633.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5909..6445
                     /gene="nusG"
                     /locus_tag="ACL8LA_001932"
     CDS             5909..6445
                     /gene="nusG"
                     /locus_tag="ACL8LA_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGS1813634.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2012 a         1123 c         1408 g         1920 t
ORIGIN      
        1 tgctcttgtt tcaattgact atagttcacg gatacagttg ggaaagaagc tattaaatat
       61 ttggagagtc cttttgggat gaacatgtca aatattacta tagatgggca agaaaagatg
      121 ggcaagaaat ggatctgagt caaacgatgc ttcttatgct caagtagtga gtctttatga
      181 tgatacaagt atctctgtta gtaataatga gacggacaaa gtgttgactg gatccctcta
      241 tactgaaaca aatgaacagg gattaacgat tccaagcagt ttactaaaaa attggaatga
      301 acagacagga aaaaatttga caactaatga tcttattggc aaatcagtct cagccagcat
      361 tgtagaaagt gctgccgaaa ctagtaagat tgctcaattt caaacgaaga ttgtacgtgt
      421 aatcaatgat gaagatgaca tggaggacag caacagtttc atgctgtctc atcaaatgga
      481 aacgattttg aaagaggctg gatttacgaa agctgtatct tattttatct tggaactcaa
      541 agatccatca cagacaaaag tagtaacaga agaattacag aaaaataaga agtatactgt
      601 gctttctcaa cagagagttc ttgatattgt gattaccttt attcgtgtta ttcagggatt
      661 attgattgtg ctttcatcac aagctattgt ggtagcagcg gttatgattg gtatcattat
      721 ttacatcaat atcatgcaac gttccaagga aataggtgtc atgaaagcag ttggttatca
      781 gaatcgtggt gtcaaaggaa tttttattta cgaggctatc tggattgtag gcatcgcctt
      841 gctgctggca tttttggttg cacaaggggt gggaagtttg gcgaatgcga ttgtaagtca
      901 cttttaccca tccatcacta aggtttttga attaaatctt ttatctgttt taggaactct
      961 agttttcgct ctattacttg gttatgtctc agcctacttc ccggcgcgta agattagtaa
     1021 aatggatcct gtagaatcgt tacgctatga ataataaggg attccttgat ttacagtctt
     1081 aaaaagaaac tcacatagaa aaacaatggc catacttgca attcaactca aatagttgta
     1141 taataggttt gttgaaaggt gatttgtagt gattcaagtt actcttttca caataaaaat
     1201 ttcatgattt tcataaggag gaaatcacta atggtagtta aagttggtat taacggtttc
     1261 ggacgtatcg gtcgtcttgc tttccgtcgt atccaaaacg tagaaggtgt tgaagttaca
     1321 cgcatcaacg accttacaga tccagttatg cttgcacact tgttgaaata cgacacaact
     1381 caaggtcgtt tcgacggtac tgttgaagtt aaagaaggtg gattcgaagt taacggtaaa
     1441 ttcgttaaag tttctgctga acgtgatcca gagcaaatcg actgggctac tgacggtgta
     1501 gaaatcgttc ttgaagctac tggtttcttt gctaagaaag aagcagctga aaaacacctt
     1561 aaaggtggag ctaaaaaagt tgttatcact gctcctggtg gaaacgacgt taaaacagtt
     1621 gtattcaaca ctaaccacga cgttcttgac ggtactgaaa cagttatctc aggtgcttca
     1681 tgtactacaa actgcttggc tccaatggct aaagctcttc aagataactt tggtgttgtt
     1741 gaaggattga tgactactat ccacgcttac actggtgacc aaatgatcct tgacggacca
     1801 caccgtggtg gtgaccttcg ccgtgctcgc gctggtgctg caaacatcgt tcctaactca
     1861 actggtgctg caaaagctat cggtcttgta atcccagaat tgaatggtaa acttgatgga
     1921 tctgcacaac gcgttccaac tccaactgga tcagttactg aattggtagc agttcttgaa
     1981 aagaacgtta ctgttgatga agtgaacgca gctatgaaag cagcttcaaa cgaatcatac
     2041 ggttacacag aagatccaat cgtatcttca gatatcgtag gtatgtctta cggttcattg
     2101 tttgacgcaa ctcaaactaa agttcttgac gttgacggta aacaattggt taaagttgta
     2161 tcatggtacg acaacgaaat gtcatacact gcacaacttg ttcgtactct tgaatacttc
     2221 gcaaagattg ctaaataatt cttgagttga tagaaagcaa ggctttgtgg tcttgctttt
     2281 ttatatggaa aaatggatga cacgatcatc cattcttttt taattctttt tcaaatgtat
     2341 ttgaaagggt agtgaaagtt agcctctcta aagtaagtgg gtgggtaaag gaaagtcgga
     2401 aggcatgaag cataagccgg cttgtctttg atttactatt atagagaggg tctcccagga
     2461 taggaagatt atgatgcaaa aggtgcacac gaatctgatg ggttcgccct gtctttagct
     2521 tgcaatgagc caaggaagtc ttgtttgaga attgctttaa tctgcttaca tgcgtttcag
     2581 catatttccc attttttgca tcaactattc tttttctacg atcatggcga tcacgtccaa
     2641 ttttgtctct gaaaacaagt tcttttctgt tgatatttcc atcaactaga gcccaatatt
     2701 ctctagaaat ctcttttttc tccaataagc gattgagaat gggcaggata aaaggatttt
     2761 tggcaaagag aactaagcca ctggtttcca tgtccagacg atgaacgaca tagcaggttt
     2821 ggccaacata ggtactgaca tggttaagaa gggcaatttc gtttggttga ttaccatgcg
     2881 ttttcatccc ctctggtttg tttacaataa tcaagtgttg atcttgataa acttcctgca
     2941 ctaagtctgg gttgccccaa gggatcgtct tttggggata atcttcctcg tcaaaagtca
     3001 actggcaaac atctccagga tttacgattt cgttccagcg gacttcttct tgatttatca
     3061 aaatatgttt cttgattctc aaaaaatgac ggatttttct agggatgagg agttgttcct
     3121 caagtaattg ctttaccgtc atttgaggta gagaggcggg taatgtaaat gtgaattgca
     3181 tacagatatt gtaacaaaaa aagccctatt tggataggaa atagctaaat tcttgtcttc
     3241 ctatgatgaa gatgataaaa taaacgcatg aaattagata aattatttga gaaatttctt
     3301 tctcttttta aaaaagaaac aagtgaacta gaggactctg attctactat cttacgtcgc
     3361 tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga ttcgaaaatt ctggcgtcgt
     3421 tatcatctaa caaagattat ccttatacta ggtttgagtg caggcttgct agttggaatc
     3481 tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt tgcaaaatgc cttgaaaact
     3541 cggactctta tttttgaccg tgaagaaaaa gaggctggtg ccttgtctgg tcaaaaggga
     3601 acctatgttg agctgactga catcagtaaa aacttgcaga atgctgttat tgcgacagaa
     3661 gaccgttctt tctataaaaa tgacgggatt aactatggcc gtttcttctt ggctattgtc
     3721 actgctggac gttcaggtgg tggctctacc attacccaac agctggctaa aaacgcctat
     3781 ttatcgcagg atcaaactgt tgagagaaaa gcgaaagaat ttttccttgc cttagaatta
     3841 agcaaaaaat atagtaagga gcaaattcta accatgtacc ttaacaacgc ttattttgga
     3901 aatggtgtgt ggggtgtaga agatgcgagt aagaaatact ttggagtttc tgcatcagaa
     3961 gtgagtctgg atcaagctgc gactctggca gggatgctca aggggccgga actgtataat
     4021 cccttgaatt ccgtagaaga ttctactaat cggcgcgata ctgtcttgaa gaatatggtt
     4081 gcagcaggat atattgataa aaaccaagaa accgaagctg ctgaagttga tatgacttcg
     4141 caattgcacg ataagtatga aggaaaaatc tcagattacc gttacccctc ttattttgat
     4201 gcggtggtta atgaagctgt ttccaagtat aatctaacag aggaagagat tgtcaataat
     4261 ggctaccgca tttacacaga gctggaccaa aactaccaag caaatatgca gattgtttat
     4321 gaaaacacat cgctatttcc gagggcagag gatggaacgt ttgctcaatc aggaagtgta
     4381 gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg gtcaagttgc tgacaatgat
     4441 aaaactggat tccggaattt caactatgca acccaatcaa agcgtagtcc tggttctaca
     4501 attaagcctt tagttgttta tacgccagca gttgaagcag gctgggcttt gaataagcag
     4561 ttggataacc ataccatgca gtataacagc tataaggttg ataactatgc agggatcaaa
     4621 acgagtcgag aagttcctat gtatcaagcc ttggcagaat cgcttaatct acctgctgtt
     4681 gccactgtta atgatttggg tgttgacaag gcttttgagg caggcgaaaa attcggactc
     4741 aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg gaagcggtgt tgaaaccaac
     4801 cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg aaggtttaat gcctgaagct
     4861 cattttatta gtagaattga aaatgctagt ggacaagtta ttgcgagtca taaaaattca
     4921 caaaaacggg tgattgataa gtctgtagct gacaagatga ccagtatgat gttggggact
     4981 ttcaccaacg gtaccggtat tagttcatcg cctgcagact atattatggc agggaaaact
     5041 ggaacaactg aagcagtttt caatccggag tacacaagtg accagtgggt aattggttat
     5101 actccggatg tagtgattag ccactggctt ggctttccta ccactgatga aaatcactat
     5161 ctagctggct ctacttcaaa cggtgcagct catgtcttta gaaacattgc caataccatt
     5221 ttaccttata cgccaggaag tacctttacg gttgaaaatg cttataagca aaatggaatt
     5281 gcaccagcca atacaaaaag acaagtacaa accaatgata atagccagac agatgataat
     5341 ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg aggctagccg ggctatctca
     5401 gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt cgatagtcaa tctatttcgt
     5461 taagatgctt gtcaaagcct agctttcttg ttataatgga taagatggag gcgttatggc
     5521 actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg agaaactatt caatcaagat
     5581 cagcggaaac cccaagccta cacgactaga agtaaataaa ttttgtaagc attgtggcaa
     5641 gtacactaca cacagagaaa cgagatagga gagagcgatg cgttttattg gagatatttt
     5701 tagacttctt aaagacacaa catggccaac tcgcaaggaa agctggagag attttcgttc
     5761 tatcatggaa tacacagctt tctttgtagt aattatttac atttttgacc agttgattgt
     5821 ttcaggtttg attcgattta ttaacatttt ttagaagatt agtggagtta attacactag
     5881 aaatcttcta tttatgaaag gaaatatcat ggatagtttt gataaaggat ggtttgtttt
     5941 acaaacttat tctggttatg aaaataaggt gaaagaaaat ctattacaac gtgcacaaac
     6001 ctacaatatg ttggataata ttctacgcgt tgaaattcca acacaaacag tgcaagttga
     6061 aaaaaatgga aagagaaaag aagtagaaga aaatcgcttt ccaggttatg ttcttgtaga
     6121 aatggtcatg acagatgaag cttggtttgt tgttcgaaac acaccaaatg ttacaggatt
     6181 tgtcggatca cacgggaaca gatcaaaacc aactccatta ttggaacaag aaattcgtga
     6241 cattttggta tctatgggac aaactgttca agaatttgat ttcgatgttg agattggtca
     6301 aactgtacgt attattgatg gtgcttttgc agactacact ggtaagatta cagaaattga
     6361 taataacaaa gtgaaaatga ttatctctat gtttggtaat gacacagttg cagaagtaaa
     6421 cctaaaccaa atcgcagaat tataaccctg agagaggctg gag
//
LOCUS       DBERYS010000072         6317 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.072, whole genome shotgun sequence.
ACCESSION   DBERYS010000072 DBERYS010000000
VERSION     DBERYS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.072"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..211)
                     /locus_tag="ACL8LA_001933"
     CDS             complement(<1..211)
                     /locus_tag="ACL8LA_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1813635.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGISNSNIRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMSNKV"
     gene            420..2189
                     /locus_tag="ACL8LA_001934"
     CDS             420..2189
                     /locus_tag="ACL8LA_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HGS1813636.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            2213..4168
                     /locus_tag="ACL8LA_001935"
     CDS             2213..4168
                     /locus_tag="ACL8LA_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1813637.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEIMKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            4170..4607
                     /locus_tag="ACL8LA_001936"
     CDS             4170..4607
                     /locus_tag="ACL8LA_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1813638.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4669..5805
                     /locus_tag="ACL8LA_001937"
     CDS             4669..5805
                     /locus_tag="ACL8LA_001937"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGS1813639.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNDFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1941 a         1064 c         1261 g         2051 t
ORIGIN      
        1 agactttatt actcatttct tgttttaaat caggagaata gtaacgattt tttccttttt
       61 tgacgaactc tattccgtaa cgatcaatca atttaatcat gtacctaata ttagaattgc
      121 ttatcccaaa tttatttgaa agcttctcta agctatatcc ttgttttcta agttcataga
      181 tctgaacttt atcatcataa gttagtttca taataaaaac accccaaaag ttagattttt
      241 tctgtctaac ttttggggtg tagttcatgt acacctgata tgatgcgttt tataattttt
      301 aagccttttt gcccagcctc gtcaaaagta atgttttgac acaaaatctg tgacaaaact
      361 ttagttttaa aggtttttaa ctttgtatat actagtttta agaaaaggag gatgatctaa
      421 tggaagaaaa agtatcattg aaagtcaggg ttcaaaaact agggacatcg ctttcaaata
      481 tggttatgcc caatattgga gcatttattg cttggggagt attgactgcc ctctttatcg
      541 ctgatggcta tctgccaaat gaacagttag ctactgttgt tggtcctatg ttaacgtatt
      601 tattgccaat cctgattggt tacacaggtg gatatatgat ccatggccaa cgtggtgccg
      661 ttgtaggagc tattgctact gttggtgcaa tcacaggttc tagtgttcct atgtttatcg
      721 gagctatggt aatgggccca ctgggaggat ggactatcaa gaaatttgat gagaagttcc
      781 aagaaaaaat tcgtcccgga tttgaaatgt tagttaataa cttctcagct ggtctcgttg
      841 gttttgcatt attgcttttg gctttctacg caatcggtcc agtcgtatcg actcttactg
      901 gagctgttgg gaatggtgtt gaggctattg tcaatgctcg cctccttcct atggctaata
      961 ttatcatcga accggctaaa gtccttttcc tcaataatgc cctcaatcat ggcattttta
     1021 ctcctctggg agtagaacag gtagctcaag ctggtaagtc aattctcttc ctattggaag
     1081 ctaatcctgg accaggcctg ggaattctat tagcttatgc tgtattcggt aaaggttctg
     1141 ctaaatcttc ttcttggggg gcaatggtta ttcatttctt cggagggatt catgaaattt
     1201 actttcctta tgttatgatg aagcctactc tatttttagc tgctatggca ggaggtatct
     1261 ctggaacttt tacttttcaa ctcttagacg ctggtcttaa atctccagct tcaccaggtt
     1321 ctattattgc gattatggct acggcgccaa aaggtgtttg gccccatcta aatgttcttc
     1381 taggtgtttt agtagcagca gttgtttctt tccttgtagc agcccttatt cttcatgcag
     1441 acaagtcaac tgaggattcg ctcgaagctg ctcaggcggc tacccaagca gctaaggctc
     1501 agtctaaagg tcagttagta tcaacttctg ttgatgcagt tgtttcgaca gactcagtgg
     1561 aaaaaatcat tttcgcctgc gatgctggta tgggaagctc tgctatggga gctagtattc
     1621 ttcgagataa ggttaaaaaa gcaggtctag agattccagt atctaatcag gcaatctcaa
     1681 atttgcttga tacaccaaaa acattaattg ttactcagga agaactgaca ccaagagcta
     1741 aagacaagag tccaagtgct attcatgttt ctgttgataa tttcttagcg tcccctcgtt
     1801 atgatgaaat tgtagcttca ttaacaggag cttctccaat agcagaaatt gaaggagata
     1861 taccaacttc agcaccagta gatagtcagg aaattgacct taaccatatt gatgctgtag
     1921 tagttgctta tggtaaagca cagggaactg caactatggg ctgtgaaacg attcgggcta
     1981 tctttagaaa caagaatatt cgtattccag tttctactgc caaaatttca gaattaggtg
     2041 aatttaattc taaaaacata atgattgtaa caactatttc tttacaggca gaagtgcagc
     2101 aagcagcacc gaattctcaa tttcttattg tggatagttt agtaacaaca ccagaatatg
     2161 acaaaatggc tgctagaatg tacaaataga actagaggtt tctaaattac gaatgctatt
     2221 aaccaaacga gaagaacaat tattgaaggc tttcctacat gtagggaagc tttcaatgca
     2281 agatatgact gaaatcttac aggtttcatc tagaacaatt tatcgaactt tatcagattt
     2341 gacagatagc atggagcaat atggaatcga aataatgaag catgggaaat actatatttt
     2401 gactggagag ttggatgatt tgccgacaga acttgaagtg ttagttgagt atagtcccca
     2461 agaaagacaa gagttgatta cctatcgcct tctgactgag agcggttttg tcaccaatga
     2521 agcattgcaa gagtgcacga aagtcagtaa tgtaactatt attcaggata tttcagatat
     2581 tgataagcgt cttttagact ttgatctgaa aattgaacga caaaaaggtt atcggatttc
     2641 tggtgattca gttggtaaga gaagattttt ggctatttta ctgacaaact gtatctcagt
     2701 agcagatttt tcaaccggta attttgggag ctttgatatt ttagaagcag atagaactag
     2761 gctggccagt cagattgtta ataagcaact gtcaggtttt ccagatatgg atgctaggat
     2821 gaagatgttt tttgcgatct tgttatctct tataggtcag gagcaaaaca ttgaaaattc
     2881 acctaatact agtaagcagg ctttggaaat ttctcaaaaa atttttcaaa cttactctaa
     2941 gcagactgca caattttata gtattcagga aattatctat tttgcgagca tcttggatga
     3001 attaatcatt aaacgtcagg acaatccgct ctttacggag aaatttgatg gtgaattttt
     3061 ctacaatatt tcaaatctga ttgatacggt ttccatgtat accaagattg acttttttaa
     3121 ggacaaggtt ttattcaatt ttcttttcca tcatattcgg ctcagtttag gcgtccctat
     3181 cctttttcag ggtgaaaatt tgccagaatc tgtccagatt ttagttgaaa ggaataaatt
     3241 tctttataca gtcatcagtc ttttagtgaa tgatattttt ccgaaatatc ttcatacaga
     3301 gtatgagtat ggcatgattg ccctacattt tatctctagc ttaggccgta gtccagagat
     3361 ttatccagtc cgtgttttgc ttttaacgga tgaacgtcgg gtcactagag atttattagt
     3421 cagtaaaatt aagagtgttg ctccttttgt agagttgata gatattcagt ctctagtaga
     3481 ttaccacagt attgatctca gtcagtatga ttatatttta tctaccaagc cgctgactaa
     3541 tcaggaaatc gatgtaattt ctagttttcc aaccgtcaaa gaattgcttg aattacagga
     3601 acgacttcag tatgtacagg cacatcgtac aattgtcgcg cgtgatgcta tcgctccaga
     3661 gaaaagttat gacttgcaag attatttaat atctagtagt cagcttttga gtcaattcga
     3721 gttggttcaa ttggagaata atcaatcatt tgagcacacg gtagaacaaa tcatccaata
     3781 tcagaagaat gtgagtgaca gagattacct aacaagaaaa ttgttatctc acttccagaa
     3841 tagtcctatg gctattccta atactggtct ggtgctttta catagtcagt ctagcaaagt
     3901 aacaacaaat agttttacta tgtttgaact caaactacct atctccgcat tgtcaatgaa
     3961 acgagaggaa gaagaggtca aaaggtgtct gctaatgcta atgtctaaag aagctagcga
     4021 ggaagcgaga gatttaatga cagctatcag tcagtcgatt attgaaaatc atctttatac
     4081 agagatttac aagacgggaa atcaatccat tatttatcag atgctaaata ctatttttaa
     4141 cgaaaaaatt aagaaattgg agaactaata tgaaacttga aaaacatttg attaagctta
     4201 ataaacaatt ttctaacaag gaggaagcta tttgttattg tgggcaagtt ctttatgagg
     4261 gtggatatgt taatgaagac tatattgaag ccatgattga gcgagataaa gagctatctg
     4321 tttacatggg taactttatc gccataccgc atggaacaga tgcagcaaaa aatgatgtcc
     4381 tcaagtctgg tattacagtc gttcaagtcc ctagaggggt tgattttggg aatgtatcta
     4441 accctcaagt ggcaacggtt ctttttggta ttgctggtat tggtaatgaa cacttagaaa
     4501 ttattcagaa aatttctatc ttctgtgcag atgtagataa tgttcttaaa ctagcagatg
     4561 ctcagtcaaa agaggaagta ttgcgcttat ttgatgctgt tgaataattg aatttcgtca
     4621 tttgtcatct agtatatatg tccctcaaat aggaaaagga gaaattgaat gaaacattct
     4681 gttcattttg gtgccggtaa tatcggtcgt ggttttatag gtgaaattct atttaaaaat
     4741 gatttccata ttgattttgt ggatgtcaat aatcagataa ttcatgctct aaatgaaaag
     4801 ggcaagtatg aaattgaaat tgcacagaaa ggacagtctc gtatagaagt aactaatgtg
     4861 gctggaatta atagcaaaga acatcctgag caagtcattg aagcgattca aaagacggat
     4921 attattacta ctgcaatcgg acctaatata ctccctttta tcgctgaact tctagccaaa
     4981 ggaatcgaag ctcgccgagt tgcaggaaat acacaggcat tggatgttat ggcctgtgaa
     5041 aatatgattg gcgggtctca atttctttat caagaagtca agaaatattt aagtccggaa
     5101 ggtttgacat ttgctgataa ctacataggt tttccaaatg ctgcagtaga caggattgtt
     5161 ccaacacaaa gtcacgaaga ttcccttttt gttatggtcg agccctttaa tgaatgggtc
     5221 gtggaaacca agcgtcttaa aaatccagat ttacgtctag aagatgtgca ttatgaagaa
     5281 gatttagaac cctttattga gcgaaaactt ttttcagtca attctggaca tgcaacttca
     5341 gcttacattg gtgcgcatta tggtgccaag acaattttgg aagctcttca aaatcctaat
     5401 atcaaatctc ggattgaatc tgtattagct gaaattcgga gtctcttgat tgccaaatgg
     5461 aactttgata aaaaagaatt ggagaattat cacaaagtca ttatagaacg attcgaaaac
     5521 cctttcatag tggacgaggt tagtcgcgta gctcgtactc caatccgaaa attaggctat
     5581 aatgaacgat tcatccggcc gatacgtgaa ttgaaagaac tcagtttgtc atataaaaac
     5641 ctacttaaaa cagttggcta tgcctttgac tatcgcgatg taaatgatga agaaagtatt
     5701 cgattaggtg aattgttggc taaacaatca gtcaaagatg ttgttataca agttacaggt
     5761 ttagacgacc aagaattgat tgagcaaatt gtagagtata tttaatcttt ttcgaaaatc
     5821 tcttcaaatc aggttagcat ccatctgcaa cctcaaaaca gtattttgag ctgacttcgt
     5881 cagttctatc tacaacctca aagcagtgct ttgagctgac tccgtcagtc ttatctgcaa
     5941 cctcaaaaca ctgtttgagt tatctgcggt aatctttcta gcttgtcttt gatttttgtt
     6001 gttatttata aggtaaaaga agctggacaa aaagtcttca aaatcggaaa aaggcagcct
     6061 atcgggtgtt caaaaatctt gataggatgt cctttattat ggaaagtctt attggatttt
     6121 ctcctcagat tgagtttttg atcagcttta tgagataggt tttgctagag atgtagccca
     6181 tcatgttatt tttatggaca gtgggaaaat tgttgaaaaa aataatgccc atcaattctt
     6241 tagtcgtcca agagaagaac gaaccaagca attttggaac gaattctttc gaatgtgatc
     6301 tatatagtaa aatgaaa
//
LOCUS       DBERYS010000073         6077 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.073, whole genome shotgun sequence.
ACCESSION   DBERYS010000073 DBERYS010000000
VERSION     DBERYS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.073"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..380
                     /locus_tag="ACL8LA_001938"
                     /pseudo
     CDS             1..380
                     /locus_tag="ACL8LA_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            547..747
                     /locus_tag="ACL8LA_001939"
     CDS             547..747
                     /locus_tag="ACL8LA_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813640.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            789..1194
                     /locus_tag="ACL8LA_001940"
                     /pseudo
     CDS             789..1194
                     /locus_tag="ACL8LA_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
     gene            1425..2132
                     /gene="ccdA2"
                     /locus_tag="ACL8LA_001941"
     CDS             1425..2132
                     /gene="ccdA2"
                     /locus_tag="ACL8LA_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS1813641.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2143..2700
                     /gene="sdbB"
                     /locus_tag="ACL8LA_001942"
     CDS             2143..2700
                     /gene="sdbB"
                     /locus_tag="ACL8LA_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGS1813642.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3074..4465
                     /locus_tag="ACL8LA_001943"
     CDS             3074..4465
                     /locus_tag="ACL8LA_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGS1813643.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4608..5525
                     /gene="adcAII"
                     /locus_tag="ACL8LA_001944"
     CDS             4608..5525
                     /gene="adcAII"
                     /locus_tag="ACL8LA_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGS1813644.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5533..>6077
                     /locus_tag="ACL8LA_001945"
     CDS             5533..>6077
                     /locus_tag="ACL8LA_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS1813645.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYT"
BASE COUNT         1877 a         1088 c         1285 g         1827 t
ORIGIN      
        1 aactaccatc cactatgcgc tcaaagctat gggttacact cgaaaaaaag aaccacacct
       61 actatgaaca agacccagaa aaagtagcct tatttcttaa gaattttaat agtttaaagc
      121 acctagcacc tgtttagatt gacgaaacag gattcgatac ttatttttat cgagaatatg
      181 gtcgctcatt aaaaggtcag ttaataagag gcaaagtatc tggaagaaga tatcagagga
      241 tttctttggt tgcaggtcta acaaatggtg aattaatcgc tccaatgact tacgaagaga
      301 tgatgacgag cgactttttt gaagtatggt ttcagaagtt tctcttacca acattaacca
      361 caccatcggt tattatagta aaatgaaata agaacaggac aaatcgatca ggacagtaaa
      421 atcgaattct aacaatgttt tagaagtcca tatgtactat tctagtttca atataccata
      481 actgttccta gtcaacctgc aagccctgtc ccagctccaa gctcagctcc gactcaagat
      541 cctagagtgg tagcaagcgc ttcggctagc tcaacgagca cacaagctca agagcaagtt
      601 gacaagtctg aacttcgtgc cttgagtcaa gagttagacc aacgcttgaa agctttggca
      661 acagtatctg atccgaaaat cgatgcaacc aaggctgtcc tcctagatgc tcaaaaagct
      721 ccggaagata gtgccttgac agagtaaggt ctgcatactg cagtagagtc tgtgaaggct
      781 gggctgcctt ggactctctc aaagatgtga aagcaaatgc tagcgacagc aagcctacac
      841 aggacaagaa ggatgcaaaa caaggaacgg aagatagtaa ggattcagat aagatgactg
      901 aaacaaactc agttccagca ggagtgattg tggtcagtct acttgccctc ctaggcgtga
      961 ttgccttctg gctaattcgc cgtaagaaag agtcagaaat ccagcaatta agcacggaat
     1021 tgaccaaggt tctaggacag ctagatgcag aaaaaagcgg ataaaaaagt ccttgccaaa
     1081 gcccaaaacc ttctccaaga aacccttgat ttcgtgaaag aagaaaatgg ctcagcagag
     1141 acagaaacta aactagtaga ggagcttaaa gcaatccttg acaaactcaa gtaaaacatg
     1201 atttatgaaa tgtcagacct aaaagacatt ttcgttcaca aaagcagcga cgagtaatcc
     1261 ttgtcgttgc tttttggcta ttttggggga taagatccgc cttgactagc aaaagaaaag
     1321 attggtccaa tagtgcccga tacgattttg cgtagcagaa agcttacaaa taaaattgaa
     1381 tatagtaaaa taggattaga attattaaga aagttggttc tttattggaa acgatagtat
     1441 ttttaatctc tgtttttcta gcaggtgttt tatccttttt ttctccttgt atttttcctc
     1501 ttctgccagt ctatgctggg attttattgg atgatcaaga aagtgcaaaa agcttttctt
     1561 tgtttgggag aaaggttctc tggtcaggct tgattcgaac actttgcttt atcgctggta
     1621 tctctctcat tttctttatt ctaggctttg gtgctggtta ctttggtcat attctctatg
     1681 caaattggtt tcgatatggc atgggagcta ttattatcat tttgggtctt caccagatgg
     1741 aaatttttca tttgaagaaa ttagaagttc aaaaaagttt tacctttaaa aaatcagact
     1801 ctaatcgtta ttggtcagct tttttacttg gtattacctt tagctttggt tggacacctt
     1861 gtattggtcc agttttaagt tctgttttag cacttgcggc ttctggaggc aatggcgctt
     1921 ggcaaggagc gatttatact ctcatttaca ctctgggcat ggcccttcct ttcttggtat
     1981 tggcactagc ttcaggtcta gtcatgccat attttagtaa aatcaagcgt catatgatgc
     2041 tactaaagaa aattggtggt ttcctcattg ttttaatggg aattttgtta ctattaggac
     2101 aagtaaatgt tctagctgga atttttgaat aaaggaggac aaatgaaaaa agtaatgttt
     2161 gctggcttaa gtctcttgtc attagttgta ttgatggcct gtggtgagga agaaactaaa
     2221 aagactcaag cagcacaaca gccaaaacaa caaacgactg tacaacaaat tgctgttgga
     2281 aaagatgctc caaacttcac attgcaatcc atggatggca aagaagttaa gttatctgat
     2341 tttaagggta aaaaggttta cttgaagttt tgggcttcat ggtgtggtcc atgcaagaaa
     2401 agtatgccag agttgatgga actagcggcg aaaccagatc gtgatttcga aattcttact
     2461 gtcattgcac caggaattca aggtgaaaaa attgttgagc aattcccaca atggttccag
     2521 gaacaaggat ataaggatat cccagttctt tatgatacca aagcaaccac cttccaagct
     2581 tatcaaattc gaagcattcc tacagaatat ttaattgata gccaaggaaa gattggaaag
     2641 attcaatttg gtgctatcag taatgcggat gcagaagcag catttaaaga aatgaactag
     2701 aatcgataaa atctgattac aagatgtagt cagattttgg taacttctaa aactaacttc
     2761 cagtttccca caacctagct tttagtatga gaaaaacgcg tgacatcagt ggaaatccgt
     2821 cttagactaa cttccactgg ttttcttttt cttgatatat aagggttcaa aagcgaaaag
     2881 actcagaaaa agtgcacgac aaatagccct aaaacagagt cgtcttagag taacttccag
     2941 ttttgggaga gagatggaag ttactttgag aagttacggt agtcagattt ttttagaaaa
     3001 acattttata aatattcaca tatctcctat atttatggta aaatagaatc atcagtttat
     3061 tttggagtca aagatgaata tatttagaac aaagaatgtt agtttagata aaacagagat
     3121 gcataggcat ttgaagttat gggatttgat tttgctgggt atcggagcca tggtagggac
     3181 aggcgtcttt acaatcacag gtactgcagc tgcaacactt gctggcccag ccctagtgat
     3241 ttcaatcgtt atttctgcct tgtgtgtggg attatcagcc ctcttttttg cagaatttgc
     3301 ctcgcgagta cccgctacag gaggtgccta tagttacctc tatgctatct taggagaatt
     3361 ccctgcctgg ttggctggtt ggttaaccat gatggagttc atgacagcca tatcaggcgt
     3421 agcttcgggt tgggcagctt attttaaagg cttgctctct caatacggga tagcctttcc
     3481 tcaggcttta aatggtacct ttaatcctca agcagggaca tttgttgatt tattgcctat
     3541 tcttgtcttg gtcttggtga cttcgcttgt tttactaaat gccaaggcag ccttacgctt
     3601 taattcgatt ctagttattt tgaaattttc cgctttagct ctctttgttt tagtaggaat
     3661 ttggaatatt aagtttgata attggagcaa ttttgctcct tacggttttg gacaaatcta
     3721 tggagctagc actggtatta tggctggtgc gtccttgatg ttcttcggtt ttttgggatt
     3781 tgaatccatc tctatggctg tagatgaagt caagactcct caaaaaaata ttcctagagg
     3841 gattgtctta tcgctttcta tcgtaaccat tctctatgcc ttggtgaccc ttgttttgac
     3901 tggtgtggtt cactatagtc atttaaatgt cgatgatgcc gttgcctttg cccttcgtag
     3961 tgttgggatt agttgggcag ccaactatgt gtcattagtg gctatcttga ccttgattac
     4021 agtttgtatt tcgatgacct atgccctatc gcgtatgatt tacagtttag cacgtgacgg
     4081 cttagtgcct gctgccttta aggaactgac gaagactagc aagataccaa agaatgctac
     4141 tatcttgaca ggtctagctt cagcagtagc agcaggaatg ttcccactag ccagtattgc
     4201 agccttttta aatatttgta ccttagccta cttgatcatg ttggcttatg gtctgattcg
     4261 cttacggaaa gaaaaaggaa tgcccaaagc tggggaattt aaaacaccat tggtacctct
     4321 gttaccaatt ttatcgatca tcatttgttt atccttcatg ttacagtata atatgaatac
     4381 ctggattgct ttcctagttg cgttgttagt aggaagtatt atttacttca cttatggcta
     4441 taagcattct accatagaag aataaagtga aagtctattg agttgttgac actcagcaga
     4501 tttttatatg tgaaagaaat ttcaaatttt ttctaaaaat atcttgacag attaaatttt
     4561 caggagtaga atatttacta gttaattaaa tggttaagga gttgttcatg aagaaacaaa
     4621 atttattttt agtcctgtta agtgtctttc ttttgtgctt gggggcttgt ggtcaaaagg
     4681 aaagtcagac aggaaagggg atgaaaattg tgaccagttt ttatcctatc tacgctatgg
     4741 ttaaggaagt atctggtgac ttgaatgatg ttcggatgat tcagtcaagt agtggtattc
     4801 actcctttga accttcggca aatgatatcg cagccatcta tgatgcagat gtctttgttt
     4861 accattctca tacactcgaa tcttgggcag gaagtctgga tccaaatcta aaaaaatcca
     4921 aagtgaaggt cttagaggct tctgagggaa tgaccttgga acgtgtccct ggactagagg
     4981 atgtggaagc aggggatgga gttgatgaaa aaacgctcta tgaccctcac acatggctag
     5041 atcctgaaaa agctggagaa gaagcccaaa ttatcgctga taaactttca gaggtggata
     5101 gtgagcataa agagacttat caaaaaaatg cgcaagcctt tatcaaaaaa gctcaggaat
     5161 tgactaagaa attccaacca aaatttgaaa aagcgactca gaaaacattt gtaacacaac
     5221 atacagcctt ttcttatcta gcgaagagat ttgggcttaa tcaacttggt attgcaggta
     5281 tctctcctga acaagaacca agtccacgac aactaacaga aattcaggaa tttgttaaga
     5341 cctataaggt taaaacgatt tttacagaaa gtaacgcttc ttcaaaagta gctgaaactc
     5401 ttgtcaaatc aacaggtgtg ggtcttaaaa ctctgaatcc tttagagtca gacccacaaa
     5461 atgacaagac ctatttagaa aatcttgaag aaaatatgag tattctagca gaagaattaa
     5521 agtgaggaaa gaatgaaaat caataaaaaa tatctagcag gttcagtggc agtccttgcc
     5581 ctaagtgttt gttcctatga acttggtcgt caccaagctg gtcaggttaa gaaagagtct
     5641 aatcgagttt cttatataga tggtgatcag gctggtcaaa aggcagaaaa tttgacacca
     5701 gatgaagtca gtaagagaga ggggatcaac gccgaacaaa ttgttatcaa gattacggat
     5761 caaggttatg tgacctctca tggagaccat tatcattact ataatggcaa ggttccttat
     5821 gatgccatca tcagtgaaga acttctcatg aaagatccga attatcagtt gaaggattca
     5881 gacattgtca atgaaatcaa gggtggctat gtgattaagg tagacggaaa atactatgtt
     5941 taccttaaag atgcggccca tgcggacaat attcggacaa aagaagagat taaacgtcag
     6001 aagcaggaac gcagtcataa tcataactca agagcagata atgctgttgc tgcagccaga
     6061 gcccagggac gctacac
//
LOCUS       DBERYS010000074         5301 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.074, whole genome shotgun sequence.
ACCESSION   DBERYS010000074 DBERYS010000000
VERSION     DBERYS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.074"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(32..262)
                     /locus_tag="ACL8LA_001946"
     CDS             complement(32..262)
                     /locus_tag="ACL8LA_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813646.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(280..921)
                     /locus_tag="ACL8LA_001947"
     CDS             complement(280..921)
                     /locus_tag="ACL8LA_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1813647.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVAFAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(933..1655)
                     /locus_tag="ACL8LA_001948"
     CDS             complement(933..1655)
                     /locus_tag="ACL8LA_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HGS1813648.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1658..1855)
                     /locus_tag="ACL8LA_001949"
     CDS             complement(1658..1855)
                     /locus_tag="ACL8LA_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813649.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNLYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1855..2061)
                     /locus_tag="ACL8LA_001950"
     CDS             complement(1855..2061)
                     /locus_tag="ACL8LA_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1813650.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2071..2535)
                     /locus_tag="ACL8LA_001951"
     CDS             complement(2071..2535)
                     /locus_tag="ACL8LA_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813651.1"
                     /translation="MIDKVVRNLLLTFFLCKMTKIINFLTTILVKKKKMCYDVSKLRE
                     KKKGAMMWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRLFQEMIGKE
                     PIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
     gene            complement(2640..3929)
                     /gene="hisS"
                     /locus_tag="ACL8LA_001952"
     CDS             complement(2640..3929)
                     /gene="hisS"
                     /locus_tag="ACL8LA_001952"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGS1813652.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETISMAAYFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4007..5254)
                     /locus_tag="ACL8LA_001953"
     CDS             complement(4007..5254)
                     /locus_tag="ACL8LA_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGS1813653.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
BASE COUNT         1738 a         1119 c          973 g         1471 t
ORIGIN      
        1 acaaaaatcc gatgaacttc accggactct tttattttga atttttgcct gctttacgct
       61 tttcagcgat ttcggctgcc tttcgaggca agacaatttc cgttatgtaa gccgtcccaa
      121 aacgcagtac acctgcaata ggagcaaaga caactgctag atagttatag aagaaatcgc
      181 ctttgaaggc ataagctagc gctccaatga tgaaaaatag aacgactgcc tgaatcactg
      241 ctaataaaat tactcgtttc atgtgacctc ctgactctat tatagcatga gaatcatcaa
      301 aaagccgact aaattattca aagcgtgaag agaaatactg tagaccagac cttttctgct
      361 aatgtaagcc aaacccaaac taaaaccaag gctaaaatag acaaaaaatt gttgcacatc
      421 acctggaaaa tgaatcaagg caaatagaag actagatacc agaagaaaaa tcagggttcg
      481 tttactattg tcctgcttag gaaagagata gcgtgctaac atccctctaa aaacaatctc
      541 ttccgtcaaa ggagcaaaaa taaccacagc aaagaatgag aaaagtggtt gagacaaggt
      601 caagtctgtc gctatttgct gatttactga aggatcatct ggcaagaaga attgaacgac
      661 cagagataag aaccaaacca agacaggaag ccaaataaat cgattaaagc cgctcttctc
      721 aatatgaaca ggagccttct gataccattt gtaaatgccg tacacatata ctccagcaaa
      781 ggccacatag agtagagtaa cagcataggg tgaagcgcct aaagcaagcg acgcagtcgc
      841 gagcccctga ataaagccat agataaataa aaaggataga agggctagaa gaatccagcc
      901 aaggttttta agtaatttca tagataactc ctttatttga aataacgttt taccataggt
      961 aactgcatca cattgatata aacatggatg gctcctacaa gcaagaaagc tagtaactga
     1021 atctctcctg tcaagaaaga aatgataata agaaaaatat ataaggctgg taagacatat
     1081 tggtgtaatt ggaataaaat tcgaaaactc tgttccaaat tagcctgacg ctccccttca
     1141 tcataagaat ttatatagtt caagacatcc tttggtgtag cgaaaaattc caaatcaaac
     1201 tgacgaacaa tcgcaatggt tttaaaaaga gatttttgag cgactaagaa taccacaaag
     1261 agtaagaaag aaaggaaaaa tgtttgaggg tttgtatgca atataatcac ctcacttaat
     1321 gaaataaaaa tagccaatgg aatcgctaca cctgtaatat taaaagcaat ggttccaaac
     1381 tcaagattcc gatacatttg cacataatag gtttcattca gatcgtcatc catttcctct
     1441 tgatacaagg aatgaaattt tctgcttttc tttaagaaat tgaaagtcaa aaacatacta
     1501 atgaaaccta tcagtaaaca aatagctgat atccatggca tcaaggcttt tacatctaaa
     1561 ataatttcgt gggattcgac acgtgcctta aacatcccta caaacatgcc caagacaata
     1621 gacatcaaaa ataacaatct acgtttcttt ttcatattca ttctcctttt tcacttgcta
     1681 gatttttgga tttcttttca atccattcaa ttactgggat gagagcaaag tagacccaaa
     1741 caaattggtc gctttgatag agattaaacc agcttaggtc catcccaatc agtagaaata
     1801 cactgactaa taaagctatg accactacat aataaatcac tttatacttg ttcatcactc
     1861 gtcctcctcc aaacgaaata ccgattcgac tgtttcgttg aaaatttgag atattttcag
     1921 ggcaatgata atggatgggg tgtactcatc ccgttctagt aggctaatgg tctgtctgga
     1981 aacccctgcc agtttggcta ggtcggtttg attgagacca tcgcgagctc taagctcttt
     2041 tagacgattt ttaagttgca tgttacacac ctactctccg tcaaattcaa cggtttggat
     2101 atcctcaatg cgttgcaact tgaatttttc ttttcccgta ttatctacac gtcgtagctt
     2161 tacccattcc tcatcaacat ccacaacttc ccagttatct ggcccaatat acactcccgt
     2221 tataattggt tcctttccaa tcatttcttg aaataatctc gacatttctg cgtttccttt
     2281 ctcttttcgc tcaagttttt tgattttatt ctctagtttc ttgatttttt tagaattatt
     2341 agaataaaag aaaatcataa atagtataaa tcctagtacc cacatcatag ctcctttctt
     2401 cttttctctt aacttgctta catcgtaaca catctttttc tttttgacaa gtatagttgt
     2461 caaaaagttt atgatttttg tcattttgca aaggaaaaag gtcaggagta agttcctgac
     2521 cactttatct atcattaata ctcttctaaa atctcttcaa accacgtcag cttcatctac
     2581 aacctcaaaa ccatgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
     2641 cattgagtat aaaatcctag tttttcaaag atttctgaga agttttggct gattgtctca
     2701 agtgacactt gcacttcttc tcgggtttgg ttgttcttga ccgtcacttg tccgctttcg
     2761 acttcgctct ctcctagggt gatgagggtc ttagccgcaa agacatctgc tgacttgaac
     2821 tgagctttga gcttacggtt gaggtaatca cgctctgctt tgaaaccttg ttggcgaaga
     2881 gcttggacta gttctagggc tttgacattt gctccatcac ccaagactgc gatatagaca
     2941 tctagggcgt tttcgatagg gagggccacg ccttgttttt caaggatgag aagcaggcgc
     3001 tctacaccaa gcccaaaacc aaatccagca gtttcagggc ctccaaagta agcaaccaaa
     3061 ccatcgtagc gaccacccgc acagactgtc aagtcattgc cctcaatctc tgtgataaac
     3121 tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca tattggtatc gatgatgtaa
     3181 tctactccaa gattttccaa catctgacgc acagcatcaa aatgagtttg gctttcttca
     3241 tcaagaaagt ccaagataga cggcgcattc tctactgcca ccttgtcttc tttttcctta
     3301 gagtccaaga cacgaagagg attttcctcc aagcgacgtt ggctatcctt agacaaggtc
     3361 tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt aggctgcacg gctctcagga
     3421 tttccaagag tgttgaggtg caatttgaca ccttgaatac cgatttcctt caaaaaatag
     3481 gctgccatag agattgtttc cacatcggta gctggattgc tagagccaaa acactcaaca
     3541 ccaatctggt ggaattggcg caagcgccct gcctgtggac gctcataacg gaacataggt
     3601 cccatatagt agaacttgct tggcttttgc acttctgggg cgaagagttt attttccaca
     3661 taggaacgga caacgggcgc agttccttct ggacggaggg taatatgacg gtcacccttg
     3721 tcataaaaat cgtacatttc cttggttacg atatccgttg tatctccgac agagcgactg
     3781 ataacctcgt aatgctcaaa aataggcgtg cgcacttctg catagttata gcgcttgaaa
     3841 atctcacggg caaagccctc aacgtactgc cacttagcag attcagcagg taaaatatcc
     3901 tgcgttcctt ttggtttttg taatttcata gggaatcctc tttaaactta atagtcttat
     3961 tttaccacaa ataaagggat taaaacagta agaaaaaaat taggatttag atatcatttt
     4021 tgagattaag aattgtcaaa aaaatagcta gcaaggaaag accaacaaat agcatccaag
     4081 tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca ggtatggata
     4141 aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga gctagatttt
     4201 tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacagg gtaaagaaga
     4261 gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact agactaagtc
     4321 ctacggtcgc ccacatcatc aatctaggca aggactgctt cccaaaataa tcattgcccg
     4381 taaggctact gatgatgatt gctactaaaa cacagaattg attgataaat agtgcctctg
     4441 tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt ccacccaaag
     4501 cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag tttttctgcc
     4561 cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg tctttgatag
     4621 aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg ctcaatttgc
     4681 tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg attcccgcaa
     4741 cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct tgaccaccaa
     4801 tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga tcatcttcag
     4861 ctactttttc cttaataaga ggcatgcgca agccacctgc aaaatcactg atgacatcac
     4921 taatgacatt gatcaaacac aggctagaaa aggcaaagag actagcttgc tgaacaacta
     4981 gggctgctag gaaaaataga accgcctgaa acaaaccgct atagaccatc catttgacct
     5041 tgtccctcgt gtaatctgcc cgaatcccta caaaaactgt aaagagggtc ggaagaatca
     5101 tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat gcatagacga
     5161 taaagaccag gttgaaaatc gaagcaccaa aagcattgaa gaagcgtgat aaagtcaaaa
     5221 ttctataagc tggatttcta aacaatagtt tcatagataa tctcccttct tgttctaagt
     5281 tctacatcaa gaatagcttt a
//
LOCUS       DBERYS010000075         5103 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.075, whole genome shotgun sequence.
ACCESSION   DBERYS010000075 DBERYS010000000
VERSION     DBERYS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.075"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            233..1778
                     /locus_tag="ACL8LA_001954"
     rRNA            233..1778
                     /locus_tag="ACL8LA_001954"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1830..1902
                     /locus_tag="ACL8LA_001955"
     tRNA            1830..1902
                     /locus_tag="ACL8LA_001955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Ala,seq:tgc)
     gene            2027..4928
                     /locus_tag="ACL8LA_001956"
     rRNA            2027..4928
                     /locus_tag="ACL8LA_001956"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5005..>5103
                     /gene="rrf"
                     /locus_tag="ACL8LA_001957"
     rRNA            5005..>5103
                     /gene="rrf"
                     /locus_tag="ACL8LA_001957"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1451 a         1029 c         1492 g         1131 t
ORIGIN      
        1 cacttgatga aaaaaagttt aaaaaagctt aaaaatcttc aaaaaagtgt tgacaagtga
       61 aagtggctgt gatatactaa tatagttgtc gcttgagaga agcaagtgac aaagaccttt
      121 gaaaactgaa caagacgaac caatgtgcag ggcgctacaa cataagttgt agtactgaac
      181 aatgaaaaaa caataaatct gtcagtgaca gaaatgagta agaactcaaa cttttaatga
      241 gagtttgatc ctggctcagg acgaacgctg gcggcgtgcc taatacatgc aagtagaacg
      301 ctgaaggagg agcttgcttc tctggatgag ttgcgaacgg gtgagtaacg cgtaggtaac
      361 ctgcctggta gcgggggata actattggaa acgatagcta ataccgcata agagtagatg
      421 ttgcatgaca tttgcttaaa aggtgcactt gcatcactac cagatggacc tgcgttgtat
      481 tagctagttg gtggggtaag ggctcaccaa ggcgacgata catagccgac ctgagagggt
      541 gatcggccac actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa
      601 tcttcggcaa tggacggaag tctgaccgag caacgccgcg tgagtgaaga aggttttcgg
      661 atcgtaaagc tctgttgtaa gagaagaacg agtgtgagag tggaaagttc acactgtgac
      721 ggtatcttac cagaaaggga cggctaacta cgtgccagca gccgcggtaa tacgtaggtc
      781 ccgagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggttaga taagtctgaa
      841 gttaaaggct gtggcttaac catagtaggc tttggaaact gtttaacttg agtgcaagag
      901 gggagagtgg aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccggtg
      961 gcgaaagcgg ctctctggct tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca
     1021 ggattagata ccctggtagt ccacgctgta aacgatgagt gctaggtgtt agaccctttc
     1081 cggggtttag tgccgtagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg
     1141 ttgaaactca aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg
     1201 aagcaacgcg aagaacctta ccaggtcttg acatccctct gaccgctcta gagatagagt
     1261 tttccttcgg gacagaggtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat
     1321 gttgggttaa gtcccgcaac gagcgcaacc cctattgtta gttgccatca tttagttggg
     1381 cactctagcg agactgccgg taataaaccg gaggaaggtg gggatgacgt caaatcatca
     1441 tgccccttat gacctgggct acacacgtgc tacaatggct ggtacaacga gtcgcaagcc
     1501 ggtgacggca agctaatctc ttaaagccag tctcagttcg gattgtaggc tgcaactcgc
     1561 ctacatgaag tcggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc
     1621 gggccttgta cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg
     1681 taaccgtaag gagccagccg cctaaggtgg gatagatgat tggggtgaag tcgtaacaag
     1741 gtagccgtat cggaaggtgc ggctggatca cctcctttct aaggataagg aactgcgcat
     1801 tggtcttgtt tagtcttgag aggtcttgtg gggccttagc tcagctggga gagcgcctgc
     1861 tttgcacgca ggaggtcagc ggttcgatcc cgctaggctc cattggtgag agatcaccaa
     1921 gtaatgcaca ttgaaaattg aatatctata tcaaatagta acaagaaaat aaaccgaaaa
     1981 cgctgtagta ttaataagag tttatgactg aaaggtcaga aaataaggtt aagttaataa
     2041 gggcgcacgg tggatgcctt ggcactagga gccgacgaag gacgtgacaa acgacgatat
     2101 gccttgggta gctgtaagta agcgatgatc cagggatttc cgaatggggg aacccaacag
     2161 gtaatacctg ttacctacat ctgttaagga tgtgaggagg aagacgcagt gaactgaaac
     2221 atctaagtag ctgcaggaag agaaagcaaa agcgattgcc ttagtagcgg cgagcgaaac
     2281 ggcagaaggg caaaccgaag agtttactct tcggggttgt aggactgcaa tgtggactca
     2341 aagattatag aagaatgatt tgggaagatc agccaaagag agtaatagcc tcgtatttaa
     2401 aatagtcttt gtacttagca gtatcctgag tacggcggga cacgtgaaat cccgtcggaa
     2461 tctgggagga ccatctccca accctaaata ctccctagtg accgatagtg aaccagtacc
     2521 gtgagggaaa ggtgaaaagc accccgggag gggagtgaaa tagaacctga aaccgtgtgc
     2581 ctacaacaag ttcgagcccg ttaatgggtg agagcgtgcc ttttgtagaa tgaaccggcg
     2641 agttacgtta tgatgcgagg ttaagttgaa gagacggagc cgtagggaaa ccgagtctga
     2701 atagggcgcc ttagtatcat gacgtagacc cgaaaccatg tgacctaccc atgagcaggt
     2761 tgaaggtgcg gtaagacgca ctggaggacc gaaccagggc acgttgaaaa gtgcttggat
     2821 gacttgtggg tagcggagaa attccaaacg aacttggaga tagctggttc tctccgaaat
     2881 agctttaggg ctagcgtcga cattagagat tcttggaggt agagcactgt ttgggtgagg
     2941 ggtccatccc ggattaccaa tctcagataa actccgaatg ccaatgaatt atggtcggca
     3001 gtcagactgc gagtgctaag atccgtagtc gaaagggaaa cagcccagac caccagctaa
     3061 ggtcccaaaa taattgttaa gtggaaaagg atgtggggtt gcacagacaa ctaggatgtt
     3121 agcttagaag cagctattca ttcaaagagt gcgtaatagc tcactagtcg agtgaccctg
     3181 cgccgaaaat gtaccggggc taaaacaatt taccgaagct gtggatacct ttataggtat
     3241 ggtaggagag cgttctatgt gtgatgaagg tataccgtga ggagtgctgg aacgcataga
     3301 agtgagaatg ccggtatgag tagcgaaaga caggtgagaa tcctgtccac cgtaagacta
     3361 aggtttccag gggaaggctc gtccgccctg ggttagtcgg gacctaagga gagaccgaaa
     3421 ggtgtatccg atggacaaca ggttgatatt cctgtactag agtatgtagt gatggaggga
     3481 cgcagtaggc taactaaagc agacgattgg aagagtctgt ctaagcagtg aggtgtgaat
     3541 tgagtcaaat gcttaattct ataacattga gctgtgatgg ggagcgaagt ttagtagcga
     3601 agttagtgac gtcacactgc caagaaaagc ttctagcgtt taaacatact ctacccgtac
     3661 cgcaaaccga cacaggtagt cgaggcgagt agcctcaggt gagcgagaga actctcgtta
     3721 aggaactcgg caaaatgacc ccgtaacttc gggagaaggg gtgctgactt aaagtcagcc
     3781 gcagtgaata ggcccaagca actgtttatc aaaaacacag ctctctgcta aatcgtaaga
     3841 tgatgtatag ggggtgacgc ctgcccggtg ctggaaggtt aagaggagtg cttagcgtaa
     3901 gcgaaggtat gaattgaagc cccagtaaac ggcggccgta actataacgg tcctaaggta
     3961 gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaatgat ttgggcactg
     4021 tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta cccgcgacag
     4081 gacggaaaga ccccatggag ctttactgca gtttgatatt gagtgtctgt accacatgta
     4141 caggataggt aggagtctaa gagatcggga cgccagtttc gaaggagacg ctgttgggat
     4201 actacccttg tgttatggcc actctaaccc agataggtga tccctatcgg agacagtgtc
     4261 tgacgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg cccaaaggtt
     4321 ccctcagaat ggttggaaat cattcgcaga gtgtaaaggt ataagggagc ttgactgcga
     4381 gagctacaac tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt ccgtatggaa
     4441 gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct cccccaagag
     4501 ttcacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct ggggctgtag
     4561 tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg gttcagaacg
     4621 tcgtgagaca gttcggtccc tatccgtcgc gggcgtagga aatttgagag gatctgctcc
     4681 tagtacgaga ggaccagagt ggacttaccg ctggtgtacc agttgtcttg ccaaaggcat
     4741 cgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt gaaacccacc
     4801 tcaagatgag atttcccatg attatatatc agtaagagcc ctgagagatg atcaggtaga
     4861 taggttagaa gtggaagtgt ggcgacacat gtagcggact aatactaata gctcgaggac
     4921 ttatccaaag taactgagaa tatgaaagcg aacggttttc ttaagttgaa tagatattca
     4981 attttgagta ggtattactc agagttaagt gacgatagcc taggagatac acctgtaccc
     5041 atgccgaaca cagaagttaa gccctagaac gccggaagta gttgggggtt gccccctgtg
     5101 aga
//
LOCUS       DBERYS010000076         4738 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.076, whole genome shotgun sequence.
ACCESSION   DBERYS010000076 DBERYS010000000
VERSION     DBERYS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.076"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(181..1929)
                     /locus_tag="ACL8LA_001958"
     CDS             complement(181..1929)
                     /locus_tag="ACL8LA_001958"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813654.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDTFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1931..3655)
                     /locus_tag="ACL8LA_001959"
     CDS             complement(1931..3655)
                     /locus_tag="ACL8LA_001959"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1813655.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3713..4651)
                     /gene="msrB"
                     /locus_tag="ACL8LA_001960"
     CDS             complement(3713..4651)
                     /gene="msrB"
                     /locus_tag="ACL8LA_001960"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS1813656.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
BASE COUNT         1409 a         1038 c          982 g         1309 t
ORIGIN      
        1 ctgacttcgt cagtcttatc tacaacctca aagctgtgct ttgagcaacc tacggctagc
       61 ttcctagttt gctctttgat tttcattgag tattacagtg aacaattttt ctagctattg
      121 taacaaaaaa gtcactcgaa ggcaactttt ttcgtccata aactttcaaa agagaatggc
      181 ttattcagaa ctaaaagccg cagctttttg catttggtaa tacttaccct ttctatccat
      241 gagttcttga tggttaccat attcaacaat atcaccatct actaagacaa gaattaaatc
      301 cgcatcctga atggttgaca aacggtgagc aatgatgaag cttgtgcggc ccttcatgag
      361 ttttgcaaag gtatcctgta ccagcacttc tgtccgtgta tcaatggaag aagttgcctc
      421 gtctaagata agaatctttg gaatagccag aaagactcgg gctatggtca agagctgagc
      481 ttggccgaca gagagagatt ctccagcatt ttccaacttg gtatcgtatc cctgtggcaa
      541 ctgttgaatg aaaaagtctg cattagctgc tttggcagca gcaattactt gctctcgact
      601 ggcttcagga ttgccaaagg caatattatc atgaatggtc ccttgtgtga gccaggtttc
      661 ttgaagcacc ataccaaact gctgtctcaa tgatgctcgt gtataatcat aaatggattg
      721 cccatccagc aagatatctc ccgagctaat gggataaaaa cgcataagga gattgataag
      781 agttgatttt ccagcacctg tcggaccaac gatggctatc ttactaccag ctggaatatc
      841 gatagacaag tccttaatca aaattttttc aggatggtag ccaaaagaga catgtttaaa
      901 ggaaatagct cccttaactt ggtcactggt caagacttcc ttacctgttt cagccacttc
      961 agggctatct aagactccat agatacgctc tacgcaagcc agagcacttt gcaactcagc
     1021 tagcactgaa gaaatatcgt taaagggctt ggtgtattgc tgaacatagt tcaaaaaagt
     1081 cactaaacga ccgacggtca aggctgaacc catcatgata cgataagctc ctactccagc
     1141 taaaagggca taaatgagtg catttacaaa gcgagtcgaa ggattgaccg ttgaagaata
     1201 aaagatggct gactgagaat agcctgagta gttgtcatga gcctcacgca atctttggat
     1261 aaattctgtt tgagcattga aggactggat tatagtctgc tggctaagcg attcttcaat
     1321 caactgagtc tgaattcccc tcgtctctgt ttgcttctgg aagagatgat aggatctctt
     1381 ggcaataaag cgtgaaatca ccatggacag tggcgtcaac agcaagacta agagagtcat
     1441 gaggagatga atttggagca tggctagaat actaaccaaa atcatcaaaa caccaatgaa
     1501 aaattggtta aaaatcatgg tcaagccagc tgccaactgt tcgatgtccg tggttacacg
     1561 actgaccatc tctccattgc cttgccgatc cgcaaaagca atcggtaacc gatggagttt
     1621 atggatgatt cgctctcgta aatctctggt ataagagaag attagacgat tatagaggag
     1681 aggattggcc cattgtacta gagtatttcc tattaccacc aagagcatct ggagaaaaat
     1741 ctgccaaaaa actggtgatg aaccagccac taggacttgg tcaatgacct gcccaatcag
     1801 aataggtaga taaattgata agccaacttg ggcaatagtt cctagaaagg ctaggaaaag
     1861 gaggaaggga tggtttgcta aatctacggc taaacgtttg agcgtctggt ttacagtttg
     1921 tcgtttcatt ctagtcctcc tttccatgtt gggatgcatt gatttcacga tagacttgac
     1981 tggatttcat caagtcatcg tgcttgccaa cagctagcaa ctcacctttt tccaagagga
     2041 gaatctggtc agccatctgt aaagtcgagg ttcgttgaga gatcaaaatt aagctcatgt
     2101 ttggaaaatt ttctctaata gctttcaaga gcttggactc ggtaatggtg tccagtgctg
     2161 aggtcgcatc atctaggatg agaaacggag cctggcgcaa gactgctcgt gcgatagaca
     2221 gcctttgttt ttgtccacct gagaaatttc gccctcctgc ttcaactaga gcatccaaga
     2281 gtccttcctt atcactgaca aaatccttag cttgagcaat ttccaaggct tgccagagtt
     2341 cttgatcagt cacttcttga tttaaaccta aagtcaagtt ggaacgaata gttcccttaa
     2401 aaagttcgac cttttgggga acataagcaa tccaagaccg ccactgctct aaattacgag
     2461 gactagatcc atctcgataa aggtcaatgc tccccttatc tattggataa agtccaagta
     2521 agagttgcac caagcttgac ttaccagaac ccgttccccc gatgatacct agaatttgtc
     2581 cttgagtcat atcaaaggaa atgtatctca gagaaggctg ggccgcatca ggataggtaa
     2641 aagtcaattc ttggacttgt aaaacccgac cactggtaac ttgcttttgt tctaattctg
     2701 aatggatgtt ttcgggagat tccgcaaaga cctcctcgat tcgcttggct gagatatagg
     2761 actggttgag agaatttatc aacatggcta gcttgaccaa ttccaccaaa atctgtaaga
     2821 ggtaattgat aagagcaatg agagcacctt gactgagcac tcctccttga attgaaatat
     2881 agccttgcca gataataacg agaagagttc cattgacaat cagataggtc agaggtgtta
     2941 ataaactaga ccagaaacct gtcttttctt gtaatctagc ataaacttgg ttaagggttt
     3001 gaaaaatctg taactctcgt ttttcttgac caaaagcacg aataacccgc atcccttgca
     3061 attgctggcg cgtttcctga accagttggt ccgttttctt tctgagacta ctgtagagag
     3121 gattgaccaa tcgagataac cctacaatga caatggtcaa aatggcaacc atgactaaga
     3181 accagaaagt caactcagct gagattcgat aagccataaa aatggcacca aaaacgataa
     3241 tgggcgctcg taaaaagaga cgcaggaatt gattgatacc agtctgaatc tggtaggtat
     3301 ccgaagtcaa gcgagtaacc aaactagaag ttgtcagacg gtctctgctg tcctttggca
     3361 aggaaagaat atgacgataa agatcgtttg tcaattcctt agcaaaacct actgctgcct
     3421 ttgctgagta aaattgagct atcaaggcca ctaaaacgcc aattactgca aagataagga
     3481 gcaggccaat ctgcatccag agatgacctt gatctccctg aggtaaagat tggtcaacaa
     3541 tcccagcaat caccatggga accaagagct caaaaacagc ttctaacagc ttgaacaagg
     3601 gggctaaaat tgattccttg atgtagggtt tgaagtaaga taataggtgt ttcataaatc
     3661 ccttctattc atattctaga aatgaagaaa gtgggaagcc ccactctctg ttttatttgt
     3721 ttaagtaagg caatagatag ccatatcctg ctttttccat ctcatccttg gccacaaagc
     3781 gtaaagaagc agaattgata cagtaacgga ggccgcctaa ctcccgcggt ccatctgtga
     3841 aaacatgacc caagtgagca ctgcctgaac gagaacgaac ttcaattcgc tccattccat
     3901 ggctcagatc cttgtaataa tgaatcaact ctttggaaat cggacggcta aaacttggcc
     3961 aaccacaacc tgaagcaaac ttatccttgg caaaaaagag tggctcacct gtcgtaatat
     4021 ctacataaat cccctcttca aaggtttggt cataggcatt ggtaaatgga gcctctgtag
     4081 cagcttcttg tgtgacacga taagactctt cagatagact ggccttcaac acctcttgac
     4141 taggcttttc atagtttgct gcatcaatca atggcttatc agcatcggtc acatcgatat
     4201 gacagtaacc tgaaggattc ttcctgagat agtcttggtg gtagtcttca gccagaatgt
     4261 agtggcgtaa ttgctccact tctactgcaa tctttcgacc cagcatgcgt tcctgctcct
     4321 gcaccactgt gtagatagct ggcaaatctg cttcatcctg ataataaatc ccagttcgat
     4381 attggcggcc acggtcattc ccttgttgat tgatagatag aggatcgata actcggaaat
     4441 aataaagtaa aatctctctg agtgacactt ccttctcatc gtaaatcact tggaccgttt
     4501 ctgcatggtc tgtttccttg atcaactggt aattggtcgt ttcgacttga ccattagcgt
     4561 agccaacact ggtttctagc actccagaaa tgcgtgaaaa atattcctct aggccccaaa
     4621 aacaaccacc tgctagataa atttctgcca tctttatttc tcctttatca cgtttttaac
     4681 taatactctt cgaaaatctc ttcaaaccac gttagcgtca ccttaccgta tatatgtt
//
LOCUS       DBERYS010000077         4572 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.077, whole genome shotgun sequence.
ACCESSION   DBERYS010000077 DBERYS010000000
VERSION     DBERYS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.077"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(58..1068)
                     /locus_tag="ACL8LA_001961"
     CDS             complement(58..1068)
                     /locus_tag="ACL8LA_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGS1813657.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1217..2386
                     /locus_tag="ACL8LA_001962"
     CDS             1217..2386
                     /locus_tag="ACL8LA_001962"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1813658.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2383..3153
                     /gene="recO"
                     /locus_tag="ACL8LA_001963"
     CDS             2383..3153
                     /gene="recO"
                     /locus_tag="ACL8LA_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGS1813659.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3150..4142
                     /gene="plsX"
                     /locus_tag="ACL8LA_001964"
     CDS             3150..4142
                     /gene="plsX"
                     /locus_tag="ACL8LA_001964"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGS1813660.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4148..4381
                     /locus_tag="ACL8LA_001965"
     CDS             4148..4381
                     /locus_tag="ACL8LA_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS1813661.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            4427..4572
                     /locus_tag="ACL8LA_001966"
                     /pseudo
     CDS             4427..4572
                     /locus_tag="ACL8LA_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1340 a          878 c         1163 g         1191 t
ORIGIN      
        1 aacatatacc tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtgtta
       61 gaaaatgccg ataagggtct gcataccaag agtggtgagg atgatggcaa tccagcagat
      121 ggctccaaga acaatggatt ttccactgga tttgaccata gcgaccagat tggttttgag
      181 accgatagca ctcatggcca tgacaataag gaatttagag agttgtttga gaggggtaaa
      241 gaaactactg gacacaccta gagaggtgag tagtgtagtg aggagagagg caaggataaa
      301 gtaaaggata aaaagtggga agactttttt cagttgtagg ctttgcttgt tttcttgttg
      361 gcgactttgc cagtaggata gaaagagcgt gatagggata atggccaaag tacgtgtgag
      421 tttaacaatg gttgcagact cgagggtatt gctttggtaa agactgtccc aagcgctggc
      481 ggcagccgtt acagaggaag tgtcgttgac cgcagtccct gcaaagaggg cgaagccttc
      541 attggataga tgaagccagg tgccgagggt tggaaagatg agcgcagcca agacattgaa
      601 gaaaaagata acggaaatgg cttgggctac ttccttttcc ttagcatgga taacaggcgc
      661 tgtcgctgca atggcagaac ccccacagat agaagaacct actccaacca aggtagccag
      721 ttttgtatcc agtgcaaaga aacgctggaa gaggtaggca ataatcagag ctattgagat
      781 agtggacagg atgacaggga gtgaagattg gccaactgcg aagacctgcg agatattgag
      841 accaaaacca agcaagacaa cggcatattg gagcaacttc ttggaactaa aggtcaaacc
      901 agcatccagt tgtttatagg acgagagaaa gggatgtagg agcatgccta tgaaaatggc
      961 aaaaacgggc gcgccaatca cagggaagaa tcctcctaag taccaagata ggatggaaat
     1021 gagaaggcag gccaagatac ctgctccatt ttttgataga aatgacataa aaacctccaa
     1081 ataaaatcta ttaccattat agacctataa acaggaaaag taaaatagaa agtggaaagc
     1141 tattctctaa tgtatttttg cggtcggggg gcttttgtag tataatagag atacgttttg
     1201 aaagtaggag gtatctatgg acttaactaa gcgctttaat aaacagttag ataaaattca
     1261 agtttcgttg attcgtcagt ttgaccaggt tatttcggag attcctgggg tcttgcgttt
     1321 gaccttgggg gaacctgatt ttacaacgcc agaccatgtc aaggaggcgg ccaagcgagc
     1381 gattgatcag aaccaatcct actatacagg gatgagtggt ctgctgactc tacgtcaggc
     1441 agccagtgac tttgttaagg aaaagtacca actggactat gctcctgaaa atgaaatctt
     1501 ggttacaatt ggggcgacag aggctttatc tgcgactttg acggctattt tggaagaggg
     1561 agacaaggta cttttgccag ctcctgctta tccaggctat gaaccgattg ttaacttagt
     1621 tggggcagaa attgttgaga ttgatacgac tgaaaatggt tttgtcttga ctcctgagat
     1681 gttggagaag gccattttgg agcagggtga taagctcaag gcggttattc tcaactatcc
     1741 agccaatccg acaggaatta cctacagtcg agagcagtta gaggccttgg cagctgtttt
     1801 acgcaagtac gaaatttttg ttgtctgtga tgaggtttac tcagaattga cctacacagg
     1861 cgaagcccat gtgtctctag gaacgatgtt gagagaccag gctattatta tcaatggttt
     1921 gtctaaatcg catgccatga caggttggcg tttggggctg attttcgctc ctgcgacctt
     1981 cacagcccag ttaatcaaga gtcaccagta cttggtcact gccgcaaata ccatggcgca
     2041 acatgctgca gtagaagcct tgacggctgg taaaaacgat gcggagccca tgaagaagga
     2101 atatatccaa cgtcgggact atatcatcga aaaaatgact gctcttggtt ttgagattat
     2161 caaaccagac ggtgccttct atatttttgc taaaattcca gcgggctaca atcaagactc
     2221 ctttgctttt ctgaaggatt ttgctcagaa gaaggccgtt gcctttatcc ctggtgcagc
     2281 ctttggacgt tacggggaag gctacgtccg cctatcttat gcagccagca tggagactat
     2341 caaagaagcc atgaaacgac ttgaggagta catgagagaa gcatgattca gtctatcacg
     2401 agtcaaggct tggtgcttta caatcgcaat tttcgtgagg atgacaagct cgtcaaaatt
     2461 tttacagagc aggttggcaa acgcatgttt tttgtcaaac acgctggtca gtctaagctg
     2521 gcgcctgtta ttcagccctt ggtgctggca cgatttctct tgcgaatcaa tgatgacgga
     2581 ctcagttaca tcgaagacta tcatgaggtc atgacttttc ccaagattaa tagtgacctc
     2641 tttgtcatgg cctatgcgac ctatgtggca gctcttgcag atgctagttt gcaggacaat
     2701 cagcaggatg ctcccttgtt tgcttttttg caaaagactt tggagttgat ggaagcaggc
     2761 ttggattatc aggttttgac caatattttt gaaattcaaa ttttgactcg atttggaatc
     2821 agcctcaatt ttaatgagtg tgtcttctgc catcgggttg gtcaggcttt tgacttttct
     2881 ttcaaatatg gagcctgcct ctgtccagag cattatcatg aggataagag acgttgtcat
     2941 ctcaatccca atatccccta tctgctcaat caatttcaag ctattgattt tgagactttg
     3001 gagaccattt cgctcaagcc tggaatcaag caagagctac gccaatttat ggatcaacta
     3061 tatgaagagt acgttgggat tcacctaaaa tcaaagaaat ttattgattc cctagcagac
     3121 tggggacaat tactaaaaga ggaaaagaaa tgaaaaaaat cgcagtagat gccatggggg
     3181 gcgattacgc acctcaggcc attgttgagg gtgtcaatca agccctatct gacttttcag
     3241 atatcgaggt tcaactttac ggagatgaag ctaaaatcaa gcaatatctg acagcgacag
     3301 agcgcgtcag cattatccat acggatgaga agattgattc ggatgatgaa cctacgagag
     3361 ctattaggaa taagaaaaat gccagtatgg tattggcagc caaggctgtc aaagatggtg
     3421 aagcagacgc tgtcctttcg gctgggaata caggtgcctt gttggcagca ggattcttca
     3481 tcgtgggtcg tatcaagaat atcgaccgtc ctggactcat gtctaccttg cctaccgttg
     3541 atggaaaagg ttttgacatg ctagaccttg gtgctaatgc agaaaataca gcccagcacc
     3601 tccatcaata tgcggttcta ggttccttct atgctaaaaa tgtccgtggc attgcgcaac
     3661 cacgcgttgg tttgctcaac aacggaacag agagtagcaa gggcgacccg cttcgtaagg
     3721 aaacttatga attactggcg gctgatgaaa gtttgaactt tatcggaaac gtggaagcgc
     3781 gtgatttgat gaatggcgtt gcagatgttg ttgtggcaga tggtttcacg ggaaacgctg
     3841 tgctcaaatc catcgaaggg acagctatgg gaatcatggg cttgctcaag acagctatta
     3901 caggtggtgg tcttcgagcg aaactaggtg ccctccttct caaggacagc ctcagtggtt
     3961 tgaaaaaaca gctcaattat tcagatgttg gtggagcggt cttgtttggt gttaaagcac
     4021 ctgttgtcaa gactcatggc tcaagcgatg ccaaggctgt ttatagtacg attcgtcaga
     4081 tccgtaccat gctagaaaca gacgtggttg cccagactgc gcgtgaattt tcaggagaat
     4141 aaaagagatg acagaaaaag aaatttttga ccgtattgtg accattatcc aagagcgaca
     4201 gggagaggac tttgtcgtga cagaatcctt gagtctgaaa gacgatttgg atgcggattc
     4261 tgttgacttg atggagttta tcttgactct ggaagatgaa tttagtatcg aaatcagcga
     4321 tgaagaaatt gaccaactcc aaaacgtagg agatgtggtt aaaatcattc aaggaaaata
     4381 gtaatcggag ttccaagtca acggaagtag atggttttta gaaatgagaa atatcggaca
     4441 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcttcaagc
     4501 acaaactcca cgtaaatcca gcaaactcaa gccgctaaca gctgaagata aagcctataa
     4561 ccatgcgcta tc
//
LOCUS       DBERYS010000078         4485 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.078, whole genome shotgun sequence.
ACCESSION   DBERYS010000078 DBERYS010000000
VERSION     DBERYS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.078"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(55..962)
                     /locus_tag="ACL8LA_001967"
                     /pseudo
     CDS             complement(55..962)
                     /locus_tag="ACL8LA_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            1218..3851
                     /gene="polA"
                     /locus_tag="ACL8LA_001968"
     CDS             1218..3851
                     /gene="polA"
                     /locus_tag="ACL8LA_001968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGS1813662.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3936..4373
                     /locus_tag="ACL8LA_001969"
     CDS             3936..4373
                     /locus_tag="ACL8LA_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGS1813663.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1221 a          833 c         1079 g         1352 t
ORIGIN      
        1 aggctccata atatccatag gggatttacc cactatggat attatggagc cattttacaa
       61 cagtgcctca aattcagata ctgacatacc taattgctgg ctggcaacct cagaagtcag
      121 aagaccttgg cgtactatgt ctaggaaggc aaaaagtttc ctttctgccc ttccttcaac
      181 tttcccacgc tctagtcctt gttcaaggcc ttcagcccta gcagtttcca aggcatagtc
      241 ctgtgctaac aagacttgtt cttctcgcat atgtagttga ctaaacattt tcctgtcctc
      301 ctcggaccag ctcttgtagt ccagcagttg atttgcttgg ctaatggctc gttgcggttg
      361 ctgggtaaag ggcttgttgc cgaaaaactc caaccatggc ttgcgaacct tgtctttgct
      421 ggtttctctg tattttttaa ttccaagaat gccatcttaa ccagatggtt ttcctgtcca
      481 ttgttggtaa tcgccaatac ctcacctgtt gtgtcttcgc gcatactaaa gctatgaaaa
      541 gccaggtcat ctgagaaata attactatcc acgatagcaa tggcgtaaac aggagcgatg
      601 tgtttgtagc tctagtgagt atcaccttct cgctgacgaa ttttttcaag attttgatta
      661 acctgactgc acaggtaagt ccacaagtga ttgatgaaaa aattctgatg atggacttga
      721 atctcaataa ttacttgagt accgttatcc aactccgcca agacgtctat actggtataa
      781 aaatcctgca ccgagtaagg catggagagt aatacgtgaa tatcgcttcc ctccaaaatg
      841 gtcacatttt ttgctggcaa gtccagcata tcgcgaataa attgacaagt gatttctgga
      901 ttgctgaata ttttcttagc aatcaaatca ttagttgggc tgatgcccgg atgtctgaga
      961 atcctcctat tcctccttct atagtgagtt gaaataagat gtgaataact ctatcaggaa
     1021 agtcaaatta atttatagaa atattttagc agtcaaggtg tactattata gattcaatat
     1081 attatattat agcacatttt taaatctagg tttactaaat tctctggctc tatctattta
     1141 ttcggaaaaa gtttgaaaaa tacttgctct agctcttccc aatggtattt tttgattctt
     1201 tcctttataa tgggtgtatg gataagaaaa aattattatt gattgatggg tcttctgtag
     1261 cttttcgggc gttttttgcg ctgtatcagc agttggaccg ttttaagaat gcggctggtt
     1321 tgcataccaa tgcgatttat ggttttcagt tgatgttgag tcatttgttg gagcgggttg
     1381 agccgagtca tattttggtg gcttttgatg cgggaaagac gaccttccgg acagagatgt
     1441 atgcggacta taagggtggt cgggccaaga ctcctgatga gtttcgtgag caatttcctt
     1501 tcattcgtga gttgctggat catatgggga ttcgtcacta tgagctggct cagtatgagg
     1561 cggatgacat cattgggacg ctggataagc tagcagagca ggatggtttt gatattacca
     1621 ttgtcagtgg ggacaaggat ttgattcagc tgacggatga gcatacggtg gttgaaattt
     1681 ccaagaaagg tgtggctgag tttgaggcct ttacgccaga ttacctcatg gaagaaatgg
     1741 gcctcacacc agctcagttt atcgatctca aggcgctcat gggtgataag tcggataata
     1801 tccctggggt gaccaaagtc ggtgaaaaga cgggtattaa gctcttgctg gagtatggtt
     1861 cgcttgaggg gatttatgaa aatattgatg gaatgaagac ttctaagatg aaggaaaatc
     1921 tcatcaatga caaggaacag gcctttttgt ctaaaacact agcgaccatt gataccaagg
     1981 caccgattgc gattggttta gaggacttgg tctatagtgg tccagatgtt gaaaatcttg
     2041 ggaaatttta cgatgagatg ggattcaaac agctcaagca ggctttaaat gtgtcgtcag
     2101 ctgatgtggc taagggtttg gattttacta ttgttgacca aatcagtcaa gatatgctga
     2161 gtgaagagtc tatcttccac tttgagcttt ttggtgagaa ttaccatacg gataatttgg
     2221 ttggatttgc ctggtcttgt gaggataagc tctatgccac agacaagctt gagctgttgc
     2281 aagacccgat tttcaaggat ttcttagaaa aaacatctct gagagtttat gactttaaga
     2341 aggttaaagt tcttttgcaa cgttttggtg tggatttgca ggcgcctgct tttgacatcc
     2401 gtttggctaa atacctcctt tcgactgtgg aggacaatga aattgcgacc atcgctagtc
     2461 tttatggtca gacttacttg gttgatgatg aaactttcta cggtaagggt gttaaaaagg
     2521 ccattcctga acgtgagaaa ttcttggaac acttagcttg taaacttgct gttttggtag
     2581 aaacagagcc tattttactt gaaaaactca gcgaaaatgg gcaattagag cttctttatg
     2641 atatggagca acctctggct tttgttcttg ccaagatgga aattgctggg attgtggtca
     2701 agaaagagac cttgcttgag atgcaggctg aaaatgagct tgtcattgaa aaactgactc
     2761 aagagattta cgagctggct ggtgaggagt ttaatgtcaa ctcgcctaag cagttgggcg
     2821 tgcttctctt tgagaaattg ggacttcctc tagaatacac taagaaaacc aagacaggtt
     2881 attcgacagc agtggatgtg ttggagcgtc tcgctcctat tgctccgatt gttaagaaaa
     2941 tcctggatta ccgtcaaatt gctaagattc aatctactta tgtaattggc ttgcaggact
     3001 ggattttggc tgatggaaag attcatactc gctatatgca ggatttgacc cagaccgggc
     3061 gtttgtctag tgtggatcca aacttgcaaa atattcctgc ccgattggaa caagggcgct
     3121 tgattcggaa ggcttttgtg ccagagtggg aggatagtgt gctactcagc tctgactatt
     3181 cacagattga attgcgcgtt ttggcgcata tttctaagga tgagcacttg attaaggcct
     3241 tccaagaggg ggcagatatc catacttcga cagccatgcg ggtctttggc attgagcgtc
     3301 ctgataatgt gactgcaaat gaccgtcgca atgccaaggc agttaacttt ggagtggttt
     3361 atgggatttc agactttggc ttgtctaata atttgggaat tagtcgtaag gaagccaaag
     3421 cctacattga tacctacttt gaacgttttc cagatattaa aaactacatg gatgaagtgg
     3481 tgcgggaggc gcgtgataag ggctatgtag agaccctctt taagcgtcgc cgtgagttgc
     3541 cagatatcaa ttcgcgcaac ttcaatattc gtggttttgc ggagcgaact gctatcaact
     3601 cacctatcca gggttcggca gcagatattc tcaagattgc catgattcag ctggataaag
     3661 ccttggttgc aggtggttat cagactaaga tgctgttaca agtgcacgat gaaatcgtcc
     3721 ttgaagtgcc taaatctgaa ttggtagaga tgaaaaaatt ggtgaaacaa accatggaag
     3781 aagccattca actcagtgtt cctcttatcg cagatgagaa tgaaggggca acctggtacg
     3841 aggctaaata aaaagggggc tagtcctcct tttttgtagt ataattctgc aaaccttttc
     3901 aaaatgtgct atactgatga aaaaggagga tttctatgag tcaagaattt atcaatccaa
     3961 gtgatggcgt gattcgtcag tatctcgcaa cgagtaaaac ccttgctgtg gtgggtttgt
     4021 ctgaccgtga agaaacaacc agcaatcgag taacaaagga aatgcaggct cggggctata
     4081 aaatcatccc agtcaatccc aaggcggcag gtggcgaaat cttgggtgaa aaggcttatg
     4141 ctagcctcgc tgaaattcct tttcctgtag atattgtcaa tgtttatcgt cgcagtgaat
     4201 ttctgcccga tgtggcgcgt gattttctca aagctgatgc caagattttt tgggcacagc
     4261 taggacttga aagtttagaa gcgaaagaga ttttgcgtga tggtggatgt gatgatatcg
     4321 tgatgaatcg ctgtatcaag agagaacata ctcgcttgat tgaggaagca taagaaaaag
     4381 gtagctgatg ggctaccttt tgtgttatac tcaatgaaaa tcaaagagca cactagcaaa
     4441 ctaggaaact agccgcgggc tgctcaaaac accgttttga ggttg
//
LOCUS       DBERYS010000079         4403 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.079, whole genome shotgun sequence.
ACCESSION   DBERYS010000079 DBERYS010000000
VERSION     DBERYS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.079"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..368)
                     /locus_tag="ACL8LA_001970"
     CDS             complement(<1..368)
                     /locus_tag="ACL8LA_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813664.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRL"
     gene            complement(365..1501)
                     /locus_tag="ACL8LA_001971"
     CDS             complement(365..1501)
                     /locus_tag="ACL8LA_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813665.1"
                     /translation="MKILSKKECESILGYLNFIGITLLFFVIISLSIYFKSYLPFQGK
                     LFLLFLVVDYATLNRYNMKLPIQSKKINTFYIRKNYIFLYLLAIVKKYYSFQILQSFI
                     YLFILYFLDIIGYFYLSITLCLIAIQIISLILPSLFSIISKLFLFLSIGLLSIEQGQV
                     VIYVILLNYFILVMGILDNHASNFYFKSSRMSQKRFRSSLFILLRFCWLNKGLTFSFI
                     LISILLSYLFWYMMTMLSIDDTKIPILFLYTSIYITLMEAIIGNHKSSIGVDLALLQL
                     SALNHSKMNFFFKNAQLMMLTHLISFLQFSLLLCLIPVTSYSNDIKSILLNLLLIPFN
                     YFIAYVYYKKALFLIKGTVSLLRWSFLVLYCFLIIFVILVGGIR"
     gene            complement(1508..1804)
                     /locus_tag="ACL8LA_001972"
     CDS             complement(1508..1804)
                     /locus_tag="ACL8LA_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HGS1813666.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(2347..3240)
                     /locus_tag="ACL8LA_001973"
     CDS             complement(2347..3240)
                     /locus_tag="ACL8LA_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1813667.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(3261..3455)
                     /locus_tag="ACL8LA_001974"
     CDS             complement(3261..3455)
                     /locus_tag="ACL8LA_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813668.1"
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPL
                     FSLIVLYMIDKRIRKKKKSL"
     gene            complement(3863..3988)
                     /locus_tag="ACL8LA_001975"
     CDS             complement(3863..3988)
                     /locus_tag="ACL8LA_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS1813669.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            complement(3991..4221)
                     /gene="blpU"
                     /locus_tag="ACL8LA_001976"
     CDS             complement(3991..4221)
                     /gene="blpU"
                     /locus_tag="ACL8LA_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGS1813670.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
BASE COUNT         1617 a          802 c          498 g         1486 t
ORIGIN      
        1 aataatcggt ataaaagata ctgactcata ccttgatacc aaataaaatt cggtatttca
       61 ataagagcat gaggtaatat tccttttatt gtagctacta ttccaatcat ctgaccagat
      121 atacccaatt gtattcctaa agatagcaaa tcaaaaagtt gcaataaggg cgaaatcgga
      181 aaacagaata gatatagcaa aaaatttgta gaatttgcct gtaaaactgt caatgtaaat
      241 tgtaattcca gtttcggagg tatagtagca aaagtctcat tttttaatat aaagcttaaa
      301 agtaaaccta gtaaaaaaca gactatacaa aataacacat agtaaatcag aaaaccactt
      361 tttctcatct aatgccccct actaaaatta caaatattat cagaaaacaa tataaaacca
      421 gaaaactcca tctcaataaa ctaactgtac ctttaattaa aaatagagct ttcttataat
      481 agacataggc tataaaataa ttgaatggaa tcaataacaa attcaacaaa atacttttta
      541 tatcattact ataacttgtc accggaatca aacataacaa caatgaaaat tgtaagaaag
      601 agattaaatg cgtcaacatc attagttgtg cattcttaaa aaagaaattc attttagaat
      661 gattaagagc agataattga agtaaagcta gatctacccc aattgaggat ttatgattcc
      721 ctattattgc ctccattaat gtaatatata ttgaggtata aagaaataat attggtattt
      781 ttgtgtcatc aattgataac atcgtcatca tataccaaaa tagataggaa agaagaatac
      841 taatcaaaat aaaagaaaaa gttaaccctt tatttaacca acaaaaacga agcaatataa
      901 atagactgct acgaaatctt ttttgactca ttcgactgct tttaaaatag aaattactcg
      961 catgattatc taatatcccc atgactaaaa taaaataatt gagtagtatg acataaatta
     1021 caacttgtcc ttgttctata gacaatagtc ctatagataa aaataagaac aatttactta
     1081 taattgaaaa caaactaggt agaattaaag aaataatttg aatagctatc aaacaaagag
     1141 taatggataa ataaaaatat ccaataatat ctagaaaata caaaataaat aaataaatga
     1201 atgattgtaa aatttggaaa gaataatatt tcttgacaat ggcaagaaga tagagaaaaa
     1261 tatagttttt ccttatatag aaagtattta tttttttact ttggataggc agtttcatat
     1321 tataacgatt caaagtagcg taatctacta ccaaaaaaag taaaaataat ttcccttgaa
     1381 aaggaagata gcttttaaaa tagattgaca aacttatgat aacaaaaaac aaaagggtta
     1441 tcccaataaa attaagataa cctaaaatac tttcacattc ttttttagaa agtattttca
     1501 tctagttcta ccatagagct gctttagcag cacctttctt tttcaatata taaagaactg
     1561 ttgctgcaat acccgaacca atagaaccta ctcctgtgat tgcacctaca attgaaatta
     1621 ctgctgtaac tgtactataa gcactaatca aattgactac agtagcagca gcttttgtcg
     1681 aaattcctaa tgtcccagcc aacataggaa aagaaacaac acctaagctt aaccaactaa
     1741 tgactgctaa cattgctgaa acaataagaa atttattatg aaattccatt cttttagatt
     1801 tcattttagt gtactccttt tgtatattat gtatgtttta ttgagagtta attcgcacat
     1861 gttgtattcc taactctctt caaatgttcg tctatatttt atactacttt tattaacata
     1921 tctataataa ataaattact actacatttt cctagattat caaattactt ttatatcact
     1981 aagactcaat aaaagataat gtctatttaa cttttatacg attactacct cctttttagt
     2041 ttacacttcc tacatcataa ataaacatac tcaaccctca caatctccaa ataactcttc
     2101 tatatcattg aaaattgagt ttcatcctct atactaatgt aggaaaccta ttaaaacatt
     2161 cctttaatcc aatataagaa taccttgttc cattaatctc tacccgatat tatagataga
     2221 accagactcc tatacttaaa aggacaaata acgttccaac cataataaac ctcctttctt
     2281 tttgagaacg ttttctttat aggtttagta tagcaaaaac atgaaaaaaa caacctgact
     2341 tagaagtcag gttgttggga aatctatctg tttttctgct aaaattctct gaactaatac
     2401 ctgatcgtct agcaattctg ctagagaaat cgccttttca tagaaacttt ctgcctctaa
     2461 aatattcttg tctacaaata aagcatattt tgcctttagc aaaaatagat gaggtcggta
     2521 aaaaacatgt cttgttttag caataatttt ctcaaatgtt tgaatatata tcagactatc
     2581 ttctgtctga atttggataa ataggctcag aagaactttt tccatttgaa ctaggctagc
     2641 cttatcttca tattctgaac atagcaatac tttttgagct aactcctcaa aatgttcttt
     2701 atcttctaat cccatagcac aacaaaagaa atacaattca ataatcaaca aatcatttac
     2761 agaaaaatat ttctttttta ataactgctg gaaatattct tccactaacc ctaaaccgta
     2821 ggtcacatct gaactaccat aaatatcaaa acgagcttgt gtcacatcca caattaactg
     2881 ctcttcctca ggcaaattct cataatagtt ttcatatatt tcctcaagta tagcttcttt
     2941 tcgttcgatt cttcccttat cagcgtaggt ttgagaatga atcagctcat ctttgagttt
     3001 taggaatcct tttggaatct ctataacctt aacatccaca atattctgta atgatactcc
     3061 aagtttatcc gcaatcaata ataatttagg aagtgttgct aaagcttgcc cattttcaat
     3121 acgtgctagc tgacgaatcg tcaactctga ttcatcacca caaatatcta tctgagtttt
     3181 tccacaagat cgcctaatag ttttgatttt ataaccaatt tcacgtttca actcatccat
     3241 agtaatactc cttaagttga ttacaaagat ttctttttct tcctaattct tttgtcaatc
     3301 atatataaaa cgataagcga aaacagcggt agaacaatat aactcttttc actcattatc
     3361 agctgaaatc ccagggttgc tagtatctta aagatgaaaa tattcatcaa aaatagaaaa
     3421 acaaatacta aaaaatattt taaaaccttc ttcatttctt ttcttcaact ttcctaattt
     3481 ctacacctat tttatcgaaa atatatataa aactccaaat aaacacttcg actgcacttt
     3541 ttttggcaaa aaatagacag aacaaacatc ctatctacta aaaatatgat atgaaaggaa
     3601 attgattttt tactgtcacc ctaggcaagc ttgaaacaaa taaatcattg atagtaacac
     3661 ttacgacatc ctatttatta cttcaatttc taatgtctct gctatttcta agctacctct
     3721 ggtatgatct attcctagtc agaatagata acaccgttct aaattacgat tcatagttac
     3781 tccttttttg actcgttact ataagactcc tctttacatg cgcaaaataa ttttgattca
     3841 gattcatcat ttatagtttt gattattttt taacagattt ttctgttttc ttctccgaag
     3901 actttaaaaa tttttttcta attaaatcat ccatataagg accaaatata ccaactacta
     3961 aaccaataat aaaactttta aaatccataa ttaccaccaa catgtcgctg cataggccac
     4021 acctccaagt atagctcctc ccgcagcacc agttgctgca ccttgccatg ttcttgtttt
     4081 aattcctagc tgaagacctc gtactgctcc tcctccaaca cctgctttgg caaaatctcc
     4141 ccaattgcat ccgccacctt caacgcaagc aagcatctca gtatccataa tttcaaattg
     4201 tgacatcttt tttgtattca taacgaatac ctctttttta tttttaatat ttgtcttgtt
     4261 acaaacttga caagtttagt ataacagtat ctattaattt ttttcatcca aatcttgaat
     4321 tggcatcgaa acgtcttgaa ttagcttttt tgtttcaaaa tcatctctat ttttaaaaaa
     4381 gatgttttct aatcactttt tta
//
LOCUS       DBERYS010000080         4136 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.080, whole genome shotgun sequence.
ACCESSION   DBERYS010000080 DBERYS010000000
VERSION     DBERYS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.080"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            74..319
                     /locus_tag="ACL8LA_001977"
     CDS             74..319
                     /locus_tag="ACL8LA_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813671.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFTKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            720..1712
                     /locus_tag="ACL8LA_001978"
     CDS             720..1712
                     /locus_tag="ACL8LA_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813672.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="ACL8LA_001979"
     CDS             1714..2532
                     /locus_tag="ACL8LA_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813673.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="ACL8LA_001980"
     CDS             2534..3319
                     /locus_tag="ACL8LA_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1813674.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="ACL8LA_001981"
     CDS             3417..3635
                     /locus_tag="ACL8LA_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGS1813675.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3781
                     /locus_tag="ACL8LA_001982"
     CDS             3620..3781
                     /locus_tag="ACL8LA_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGS1813676.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
BASE COUNT         1153 a          703 c          896 g         1384 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat taatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta caaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tatttcatga
      541 gagaaatcct aatttcacaa tctataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttctgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aacattttgt gaagattgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg agcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca aaagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctatc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttcg tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttaatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgtt gaggttttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagg ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt tctcagctat ggaatccgta
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgccatac tgtaagtgta atcgccgatt tagctcagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggagttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttattctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatcccggtt aacgaaagtt ttggacttat actcat
//
LOCUS       DBERYS010000081         4051 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.081, whole genome shotgun sequence.
ACCESSION   DBERYS010000081 DBERYS010000000
VERSION     DBERYS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.081"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            371..598
                     /locus_tag="ACL8LA_001983"
     CDS             371..598
                     /locus_tag="ACL8LA_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813677.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            886..3768
                     /gene="nanA"
                     /locus_tag="ACL8LA_001984"
     CDS             886..3768
                     /gene="nanA"
                     /locus_tag="ACL8LA_001984"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGS1813678.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKISRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQKIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1381 a          670 c          886 g         1114 t
ORIGIN      
        1 gtcagtctta tctacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt
       61 ttgctctttg attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac
      121 tataaatgtt cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa
      181 ctaaaatcta ctatattaca ctatattgaa agcgttttaa aaatgaggta taataaattt
      241 actaacgctt ataaaaagtg atagaatcta tctttatgta tatttaaaga tagattgctg
      301 taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa gcttagaaaa
      361 ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa gacaagttga
      421 actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc acttatttgg
      481 gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat ttcccattat
      541 ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata aggattgagc
      601 aggaagtatg gtgtaaatag cataggctga tgtccatcat ttgcttataa agagatattt
      661 tagtttaatt gcagcggtgt cctggtagat aaactagatt ggcaggagtc tgattggaga
      721 aaggagaggg gaaatttggc accaatttga gatagtttgt ttagttcatt tttgtcattt
      781 aaatgaactg tagtaaaaga aagttaataa aagacaaact aagtgcattt tctggagtaa
      841 atgtcttatt tcagaaatcg ggatatagat atagagagga tcagtatgaa tcggagtgtt
      901 caagaacgta agtgtcgtta tagcattagg aaactatcgg taggagcggt ttctatgatt
      961 gtaggagcag tggtatttgg aacgtctcct gttttagctc aagaaggggc aagtgagcaa
     1021 cctctggcaa atgaaactca actttcgggg gggagctcaa ccctaactga tacagaaaag
     1081 agccagcctt cttcagagac tgaactttct ggcaataagc aagaacaaga aaggaaagat
     1141 aagcaagaag aaaaaatttc aagagattac tatgcacgag atttggaaaa tgtcgaaaca
     1201 gtgatagaaa aagaagatgt tgaaaccaat gcttcaaatg gtcagagagt tgatttatca
     1261 agtgaactag ataaactaaa gaaacttgaa aacgcaacag ttcacatgga gtttaagcca
     1321 gatgccaagg ctccagcatt ctataatctc ttttctgtgt caagtgctac taaaaaagat
     1381 gagtacttca ctatggcagt ttacaataat actgctactc tagaggggcg tggttcggat
     1441 gggaaacagt tttacggtaa ttacaacgat gcacccttaa aagttaaacc aggtcagtgg
     1501 aattctgtga ctttcacagt tgaaaaaccg acaccagaac tacctaaagg ccgagtgcgc
     1561 ctctacgtaa acggggtatt atctcgaaca agtctgaaat ctggcaattt cattaaagat
     1621 atgccagatg taacgcatgt acaaatcgga gcaaccaagc gtgccaacaa tacggtttgg
     1681 gggtcaaatc tacagattcg gaatctcact gtgtataatc gtgctttaac accagaagag
     1741 gtacaaaaac gtagtcaact ttttaaacgc tcagatttag aaaaaaaact acctgaagga
     1801 gcggctttaa cagagaaaac ggacatattc gaaagcgggc gtaacggtaa accaaataaa
     1861 gatggaatca agagttatcg tattccagca cttctcaaga cagataaagg aactttgatc
     1921 gcaggtgcag atgaacgccg tctccattcg agtgactggg gtgatatcgg tatggtcatc
     1981 agacgtagtg aagataatgg taaaacttgg ggtgaccgag taaccattac caacttacgt
     2041 gacaatccaa aagcttctga cccatcgatc ggttcaccag tgaatatcga tatggtgttg
     2101 gttcaagatc ctgaaaccaa acgaatcttt tctatctatg acatgttccc agaagggaag
     2161 ggaatctttg gaatgtcttc acaaaaagaa gaagcctaca aaaaaatcga tggaaaaacc
     2221 tatcaaatcc tctatcgtga aggagaaaag ggagcttata ccattcgaga aaatggtact
     2281 gtctatacac cagatggtaa ggcgacagac tatcgcgttg ttgtagatcc tgttaaacca
     2341 gcctatagcg acaagggtga tctatacaag ggtaaccaat tactaggcaa tatctacttc
     2401 acaacaaaca aaacttctcc atttagaatt gccaaggata gctatctatg gatgtcctac
     2461 agtgatgacg acgggaagac atggtcagcg cctcaagata ttactccgat ggtcaaagcc
     2521 gattggatga aattcttggg tgtaggtcct ggaacaggaa ttgtacttcg gaatgggcct
     2581 cacaagggac ggattttgat accggtttat acgactaata atgtatctca cttaaatggc
     2641 tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa aaacttggca tgctggagaa
     2701 gcggtcaacg ataaccgcca ggtagacggt caaaagatcc actcttctac gatgaacaat
     2761 agacgtgcgc aaaatacaga atcaacggtg gtacaactaa acaatggaga tgttaaactc
     2821 tttatgcgtg gtttgactgg agatcttcag gttgctacaa gtaaagacgg aggagtgact
     2881 tgggagaagg atatcaaacg ttatccacag gttaaagatg tctatgttca aatgtctgct
     2941 atccatacga tgcataatgg aaaagaatac attatcctca gcaatgcagg tggaccgaat
     3001 cgtgaaaatg ggatggtcca cttggcacgt gtcgaagaaa atggtgagtt gacttggctc
     3061 aaacacaatc caattcaaaa aggagagttt gcctataatt cgctccaaga attaggaaat
     3121 ggggagtatg gcatcttgta tgaacatact gaaaaaggac aaaatgccta taccctatca
     3181 tttagaaaat ttaattggga atttttgagc aaagatctga tttctcctac cgaagcgaaa
     3241 gtgaagcgaa ctagagagat gggcaaagga gttattggct tggagtttga ctcagaagta
     3301 ttggtcaaca aggctccaac ccttcaattg gcaaatggta aaacagcgac tttcctaacc
     3361 cagtatgata gcaagacctt gttgtttgca gtagataagg aagatatcgg acagaaaatt
     3421 attggtatag ctaaaggaag catcgaaagt atgcataatc ttcctgtaaa tctagcaggt
     3481 gccagagttc ctggcggagt aaatggtagc aaagcagcgg tgcatgaagt tccagaattt
     3541 acagggggag ttaatggtac agagccagct gttcatgaaa tcgcagagta taagggatct
     3601 gattcgcttg taactcttac tacaaaagaa gattatactt acaaagctcc tcttgctcag
     3661 caggcacttc ctgaaacagg aaacaaggag agtgacctcc tagcttcact aggactaaca
     3721 gctttcttcc ttggtctgtt tacgctaggg aaaaagagag aacaataaga gaagaattct
     3781 aaacatttga ttttgtaaaa atagaaggag atagcaggtt ttcaagcctg ctatcttttt
     3841 ttgatgacat tcaggctgat acgaaatcat aagaggtctg aaactacttt cagagtagtc
     3901 tgttctataa aatatagtag attgaaataa gatgtgaaca actctatcag gaaagtcaaa
     3961 ttaatttata gaaaaatttt agtagtcaag gtgtactgtt atagattcaa tatattatgt
     4021 gactattaac cttgtcttct cctaaaattg a
//
LOCUS       DBERYS010000082         3813 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.082, whole genome shotgun sequence.
ACCESSION   DBERYS010000082 DBERYS010000000
VERSION     DBERYS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.082"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..418
                     /locus_tag="ACL8LA_001985"
     CDS             <1..418
                     /locus_tag="ACL8LA_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGS1813679.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="ACL8LA_001986"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="ACL8LA_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGS1813680.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1429 a          626 c          747 g         1011 t
ORIGIN      
        1 taaaaataag gcagaccaag acagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact ctg
//
LOCUS       DBERYS010000083         3445 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.083, whole genome shotgun sequence.
ACCESSION   DBERYS010000083 DBERYS010000000
VERSION     DBERYS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.083"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..270
                     /locus_tag="ACL8LA_001987"
     CDS             <1..270
                     /locus_tag="ACL8LA_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813681.1"
                     /translation="VTHYKYYRAENSHLIYADYFEMKLQKLLKDDTKVFEKSTFKFVE
                     GYKIYLTESKESGIKQMDNVIKYFEFIESKSIALYFQKRLNELID"
     gene            complement(628..1161)
                     /locus_tag="ACL8LA_001988"
     CDS             complement(628..1161)
                     /locus_tag="ACL8LA_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGS1813682.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1250..2026)
                     /gene="recX"
                     /locus_tag="ACL8LA_001989"
     CDS             complement(1250..2026)
                     /gene="recX"
                     /locus_tag="ACL8LA_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGS1813683.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2064..3419
                     /gene="rlmD"
                     /locus_tag="ACL8LA_001990"
     CDS             2064..3419
                     /gene="rlmD"
                     /locus_tag="ACL8LA_001990"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS1813684.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1133 a          580 c          603 g         1129 t
ORIGIN      
        1 gttacccact acaaatatta tagagccgaa aattcacatc taatatatgc agactacttt
       61 gaaatgaaat tacaaaaatt attaaaagat gacacaaaag tttttgaaaa atctacattc
      121 aaatttgtag aaggatataa aatatacctg acagaatcta aagaatctgg aattaaacaa
      181 atggacaatg tcataaaata ttttgagttt attgaatcta aaagtattgc tttatatttt
      241 caaaaacgat taaatgagct gatagattaa atatcatttt ctctgttaag atattgtttt
      301 taaaatattg tactaaatga ttgatgctat gtggaaatac aaaaaaatgt ttttgatacg
      361 aagttgacct gtatttttta tactaatcat tttcgtattt tttgtattaa acgatataag
      421 tttgttgtaa acttactagg aataaagaca ttaaaaaata atagtatatc tatttgtttt
      481 atatatttta cgaattctgc ataaatctct ttctagtaat gtgttgtaac tctgctataa
      541 tggatttatt cctttttgta tttacacaat ttattttata ctaccaaaaa aacaaggtca
      601 ggattttgtt cctgaccttt gacaacttta ccgattcttt agttctacat agcgcttgta
      661 ccaaatgttt acataggctt ctgagaaagg accacgtcca ttgttaatcc aatcaacaag
      721 aattttgaca tgttctttta aaatatagtc caagtcatca gaataattca ttttgcgttt
      781 gtgacgctcg tattcttcaa cgtccaagag acgtttttcc ccatctgtaa aaattttaac
      841 atccaaatcg taatcaatat acttcagtgc ttcttcatcc agatagtagg ggctagccat
      901 attgcaatag taagaagttc cattatcacg aatcatggca atgatattaa accaatattt
      961 cttgtgaaag taaacaatag ccggttctcg agtgacccaa cgacgaccat cactttcggt
     1021 aacaagtgta tgatcgttga caccaataat ggcgttttct gttgttttta gtaccatggt
     1081 gtcccgccaa gttcggtgga gactcccatc atgcttataa ctttgaattg taataaagtc
     1141 gccttctttt ggaagcttca taactaacca actttctaca atttataagt ttatcattta
     1201 ctattgtacc ataaaattac ctaaaatctg tgaatttcac ttggaaatat taaagatatt
     1261 ctctaagagc gcttgctata tccgaaaaat cgtagccctt tcgtgctaaa acttgagtta
     1321 aacgctgctt cagttcgtat ccttcatact ttcgagcata cttagtatat tgcttatcaa
     1381 gttccttgaa gatgagttct tgagtcgttt cttggtcaac ttggctatct aagtcgtcaa
     1441 aggcaatttt agcatcagaa taagagaagc ccttgttagt caagttctgg ataatcttat
     1501 cttgcagggc acgagctgga agttttccct catatttttt caatagttta ttggctacac
     1561 gttgagcaac ttcagaaaaa tcaaattctt tcaagttctc ttctatagta gattttgaaa
     1621 tccctttttg agatagtttc tgagtcagca cataaggacc cttgtctcct gaaagttgat
     1681 tggtattgat gatagcataa gcgtactgac catcattaat ccacttatct tctttaagat
     1741 tagcaataac ttgagaaacg atgtttttat caatatcgta ttttttcaga tattctctga
     1801 cttctttttc agtgcgtgct ttaaaggata agtggtagag agcgagattc ttaccataag
     1861 aaaattgagc aaagtcttga atctctttca attcctcttc gcttatcacc ttatctctcg
     1921 ataacataaa acgaacaatt gtatcttcgg tgatatagca tttgtcgcca ttatcaagct
     1981 ccatcaggta gagtcttttt ttcttttcaa gttttgtgat tttcatagct ctattataac
     2041 tcaaaatgtg ataagatagg ggtatgaatc tgaaagtgaa acaaaaaata ccattaaaaa
     2101 tcaagcgcat gggaattaac ggtgagggaa tcggctttta ccaaaaaaca ttagtctttg
     2161 taccaggagc tctcaaaggc gaagatatct attgtcagat tacttctatt agacgcaact
     2221 ttgttgaagc aaaattactg aaggtcaaca agaagtctaa atttcgaatt gtgccatctt
     2281 gtactattta taatgaatgc ggaggctgcc aaatcatgca cctgcattat gataagcagc
     2341 tggagttcaa gacggactta cttcatcaag cgctgaaaaa atttgctcct gcaggatatg
     2401 aaaattatga aattcgtcca actattggaa tgcaggaacc aaaatattac agagctaagt
     2461 tacaatttca gactcgaaaa tttaaaaatc aggtcaaggc gggcttatat gcacaaaact
     2521 ctcactattt agtagagttg aaagactgcc tggtacaaga taaggaaacc caagtgattg
     2581 ctaatcgctt agcagaatta cttacttatc accagattcc aatcacggat gagagaaaag
     2641 ttctaggtgt ccgtactatt atggtccgac gcgcgagaaa gaccggacag gttcagatta
     2701 ttattgttac aaaccgccag cttaatttaa ctcaattggt aaaagagttg gttaaagatt
     2761 tcccagaagt tgtgacagta gctgttaata caaatacagc taaaaccagt gagatatatg
     2821 gtgaaaagac agagattatc tgggggcaag agagtattca agaaggtgta ctcaattatg
     2881 aattttcact atcccctcga gctttttatc aactaaatcc tgagcaaaca gaagtcctct
     2941 atagcgaagc agtaaaagcg ctggatgttg ataaagaaga ccatttgatt gacgcttatt
     3001 gtggagttgg aacgattgga tttgcctttg caaagaaagt taaaacactc agaggtatgg
     3061 atattattcc agaagctatt gaagatgcca agcgaaatgc taaaagaatg ggatttgaca
     3121 atactcatta tgaagctgga acggcagaag agattattcc tcgttggtac aaggaaggct
     3181 accgagcaga tgctttgatt gttgacccac cacgtacagg tctggatgat aagttattag
     3241 atactattct tacttatgta ccagaaaaaa tggtttatat ttcttgtaat gtttcgacct
     3301 tggctcgtga tttggtacgc ttagtagaag tctatgatct tcattatatc cagtcggtcg
     3361 atatgttccc acatacagct cgaactgaag ctgttgtaaa attaataaaa aaagtttaaa
     3421 aaagtagttg acaaagtttg aaaag
//
LOCUS       DBERYS010000084         3096 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.084, whole genome shotgun sequence.
ACCESSION   DBERYS010000084 DBERYS010000000
VERSION     DBERYS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.084"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1242
                     /locus_tag="ACL8LA_001991"
     CDS             join(<1..332,332..1242)
                     /locus_tag="ACL8LA_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1813685.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYPKKVKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1477..2085)
                     /locus_tag="ACL8LA_001992"
     CDS             complement(1477..2085)
                     /locus_tag="ACL8LA_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HGS1813686.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2263..3021
                     /locus_tag="ACL8LA_001993"
     CDS             2263..3021
                     /locus_tag="ACL8LA_001993"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS1813687.1"
                     /translation="MADYILVRDSEIVYETSSEIVSKVKVGEGTLNRFCKKIGYSGFQ
                     ELKLKMTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIELAIKLIREAN
                     QILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDLIIAFSLSGS
                     TKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGGSLMAKASQL
                     FIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT         1174 a          395 c          571 g          956 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccca acttagaaca agtgaagagt
      901 atgttagaac aagcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaaggg aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttgaaatcgg agatgtttta tggttatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggacttagtt ctgtgcagta cagaactaaa tcctttggat aaattatttg tctaattttt
     1261 tggggtcagt acagtattaa ataccatgat cttatgcgtt ttataataaa aaatctgttc
     1321 ttgttttatt tttagaaaat ttactaaaga ctaaaaatat tatgaataat ttaaaaaata
     1381 gtcaggttat atagtttttt gaaattaaaa ttattttatc gttgaaatta gttaaaaatt
     1441 tttataaaat caagatgcaa ggctatgaga tgttctctag tttaattttt catacttcga
     1501 gaatataaag ttatataaaa atgcattagc atattttgtt attgagggaa gtataaaaat
     1561 cattaaagtt atccaaacta gtaattgatt ctgtaatata aagaagagta gaactgggat
     1621 aatttcaata attctaataa gaacggcaat aaaaaaatgt ttataggcta acatgattga
     1681 attgtgtaga gaatttttta aagtgttatt atatctaccg attaaaggaa ttagaaatgt
     1741 tgttgctatt aaatgaagta tgatatatat tactccaata tgtcgtaaga tagaaatgat
     1801 atatataggt aatggtatgt agggtaggaa gaacaaactg aataataaga ttaaaattgg
     1861 taaattagat attaatgctg ctcctaaatt tttccaataa tctttaaaaa aactgtttgc
     1921 gaagtctcta tagggatagg attcctcgtg tcgtacaatt tttattagag ttgctattag
     1981 ggcactttca cctgatccga tagcaataac tgttactact gacgctacat agagtagatt
     2041 taacattata agatttgaaa ctttatcaat aaattttatc tgcatcttta actcctgctg
     2101 tttttatact tataatatca catcatttga gtttcatcaa ctttgaatac attaagaaag
     2161 aatataacta tgaggaaagg aaaaaattat tatgaataaa tccaaaaaat ataaagaaag
     2221 aattcattat gaatacgata atcttactaa atcagagaaa aaattgctga ctatatttta
     2281 gttagagata gtgaaatagt ttatgaaacg tcttcagaaa ttgttagtaa agtaaaagtt
     2341 ggagagggaa ctcttaatcg attttgcaaa aagattggat actctggttt tcaggaacta
     2401 aaattaaaaa tgacgaaaga tatattagag ttagaatctc aaaaaatgag tagtgacaca
     2461 tttattgatg aaataaaaaa taattattta tctattgtgg aaagtacaag aaaattaatt
     2521 gatggtagac aaatagaatt agctataaaa ttaataagag aagcaaacca gattttaatg
     2581 attggtgtgg gttctagtgg aaatgcggct agagaatttg agagtagttt gttaagaatt
     2641 ggaataataa gtaagacggt aattgatacc cattttcaac tcatgcatac tgcgttattg
     2701 aaagataatg atttgataat agcgtttagt ttatctggtt ccacaaaaga agttgaggaa
     2761 actttattaa acgcaaaaag aaaaaatgtt aaaattattt caataacaaa ttattcgtcc
     2821 agaaatatag caaaattatc tgactgcgtt cttttgacat caaaaaaaga atcttattta
     2881 gagggtggta gtttaatggc taaggcttct caactattca taattgatgt aatatgcact
     2941 agactctctt taataaatta tgaagatact atatgtaaaa aagaagagat tgcttctttg
     3001 ctgtcaaata aagtggaata aatcaataat ttttaaattt taataaaaat tttttaaaat
     3061 aaattaaaaa acgatcctaa aagggtcgtt tttttg
//
LOCUS       DBERYS010000085         3079 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.085, whole genome shotgun sequence.
ACCESSION   DBERYS010000085 DBERYS010000000
VERSION     DBERYS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.085"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            117..920
                     /locus_tag="ACL8LA_001994"
     CDS             117..920
                     /locus_tag="ACL8LA_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1813688.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     EVADRIIFMADGEVLVDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            960..1724
                     /locus_tag="ACL8LA_001995"
     CDS             960..1724
                     /locus_tag="ACL8LA_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS1813689.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     EFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1737..2414
                     /locus_tag="ACL8LA_001996"
     CDS             1737..2414
                     /locus_tag="ACL8LA_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1813690.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2424..>3079
                     /locus_tag="ACL8LA_001997"
     CDS             2424..>3079
                     /locus_tag="ACL8LA_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1813691.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     "
BASE COUNT          843 a          737 c          595 g          904 t
ORIGIN      
        1 cgatcgattt ttcctgttct tatttcacta tagtgaattt tttcagatat ggtttgacaa
       61 gaacattctt ttattgcaga attgtgttat aaaatctaac ataaaagagt tctctcatgg
      121 ctttagtaga atttaaaaac gtcgaaaaat attacggaga ctaccacgca ctccgcaaca
      181 tcaatctccg ttttgaaaaa ggacaagttg ttgtcctgct tggaccttct ggctctggga
      241 agtccactct tatccgtacg atcaatggtt tagaggctgt tgacaaagga agtctcctag
      301 tcaatgggca ccaagttgct ggtgccagcc agaaagattt ggtacctctt cgcaaggaag
      361 tcggcatggt ttttcaacat tttaaccttt atccacacaa aacagtgtta gaaaacgtga
      421 cacttgcacc cattaaagtt ctaggaattg ataaaaaaga agctgaaaaa accgccaaaa
      481 aatatctgga atttgtaaat atgtgggaca agaaagattc ctatcccgcc atgctatctg
      541 gtggacaaaa acagcggatc gccatcgctc gtggtcttgc tatgcatccg gaactcctcc
      601 tctttgatga accaacatct gctcttgatc ctgagactat cggagatgtt ctagcagtta
      661 tgcagaaact ggcgcgtgat gggatgaaca tgatcatcgt tacccacgaa atgggctttg
      721 ctcgagaggt tgcggaccgc attatcttta tggccgacgg agaagtttta gaggttgcgg
      781 accgcattat ctttatggcc gacggagaag ttttagtaga tacgacagat gtcgataact
      841 tttttgacaa tccaagcgaa cctcgtgccc aacaattcct cagcaaaatt atcaaccacg
      901 aaagtgacaa agtcaaataa ggaggcgcct atgaaaaaaa attcttttta tcagcattat
      961 tgattagcct tttcggcctt gctgctgcca aaccagtcca ggctgataca agtatcgcag
     1021 acattcaaaa aagaggcgaa ctggttgtcg gtgtcaaaca agacgttccc aattttggtt
     1081 acaaagatcc caagaccggt acttattctg gtatcgaaac cgacttggcc aagatggtag
     1141 ctgatgaact caaggtcaag attcgctatg tgccggttac agcacaaacc cgcggccccc
     1201 tcctagacaa tgaacagggc gatatggata tcgcgacctt taccatcacg gacgaacgca
     1261 aaaaactcta caactttacc agtccctact acacagacgc ttctggattt ttggtcaata
     1321 aatctgccaa aatcaaaaag attgaggacc taaacggcaa aaccatcgga gtcgcccaag
     1381 gttctatcac ccaacgcctg attactgaac tgggtaaaaa gaaaggtctg aagtttaaat
     1441 tcgtcgaact tggttcctac ccagaattga ttacttccct tcacgctcat cgtatcgatg
     1501 ccttttccgt tgaccgctct attctatctg gctacactag taaacggaca gcactactag
     1561 atgatagttt caagccatct gactacggta ttgttaccaa gaaatcaaat acagagttca
     1621 acgactatct tgataacttg gttactaaat ggagcaagga tggtagtttg cagaaacttt
     1681 atgatcgtta caagctcaaa ccatctagcc atactgcaga ttaaggagga caccccatga
     1741 cagatttatc atcttggaca gcctattttc aggattttgg acaatttttc aatggtttcc
     1801 tcttcaccct tgccctagcg attggatcct ttatcctcgc catggtctta ggaatcttct
     1861 ttggtgccat gtcaaccagt aaacgtccaa ttttacgcat tttagctcgc atctttgtgg
     1921 aattttacca aaacactccc ctcttggtgc agtttgttat tgttttttat ggcctacctc
     1981 ttatcagtga ccatactatc atgattccga tttattggac agctgttctc tgcgtgggac
     2041 tctatcacgg cgcttatatc gctgaggtta ttcgttcagg gattcagtct attcctagtg
     2101 gtcagatgga ggccgcctta tcgcaaggtt ttacctatat cagtgccatg cgcttgatta
     2161 tcttgcctca gaccttccgc atcattctcc ctccattgac caaccaaatt gttaacctca
     2221 tcaagaacac ctctactgta gctatcatct ctggagtaga cttgatgttt gtgactaagt
     2281 cttggtcggc tctcaacgga aactatatcc cagccttttt aggtgctgct cttctctact
     2341 ttgccctatg cttccctgtt gcccagtttg gtcgcaagat ggagcaagcc aacaaaaaag
     2401 cctattcact ttaggaggtt actatggaat ctattttaga agttttaacc ccagataacc
     2461 tagtctttat ctttaaagga tttggcttga ccctctatat ttctctgatt gccatcatcc
     2521 tctctactat catcggtacg gtgctagctg tcatgagaaa tggcaaaaat tctgtcttgc
     2581 gaatcatctc cagtatttat atcgagtttg tgcgcaacgt tcccaacctt ctctggattt
     2641 ttactatctt tttggtgttc aaaatgaaat ccacaccagc aggtattaca gcctttactc
     2701 tctttacatc agcagccttg gctgagatta ttcgaggcgg tctcaatgcc gtagacaagg
     2761 gacaatacga agcaggaatg tcacaaggct tcacctcagc ccaaatcctc tactacatca
     2821 ttctcccaca agccatccgc aaaatgctac cagccatcat ttctcagttt gttaccgtga
     2881 ttaaggatac cagtctcctc tactctgtta tcgccctaca agaactcttt ggagccagcc
     2941 aaattctcat gggccgttat ttcgaaccag agcaggtctt cagtctttac atcctgattg
     3001 ccctcatcta cttcagcttt aacctagcaa tttctagcct gtctcatatg ctagccaaac
     3061 gttggcaaca agccgcaga
//
LOCUS       DBERYS010000086         2952 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.086, whole genome shotgun sequence.
ACCESSION   DBERYS010000086 DBERYS010000000
VERSION     DBERYS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.086"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..436)
                     /locus_tag="ACL8LA_001998"
                     /pseudo
     CDS             complement(1..436)
                     /locus_tag="ACL8LA_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            773..2119
                     /gene="gdhA"
                     /locus_tag="ACL8LA_001999"
     CDS             773..2119
                     /gene="gdhA"
                     /locus_tag="ACL8LA_001999"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGS1813692.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2625..2795)
                     /locus_tag="ACL8LA_002000"
     CDS             complement(2625..2795)
                     /locus_tag="ACL8LA_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813693.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2808..>2952)
                     /locus_tag="ACL8LA_002001"
     CDS             complement(2808..>2952)
                     /locus_tag="ACL8LA_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HGS1813694.1"
                     /translation="IVAHRLSTIKDADVIFVMKGGKIVESGNHKYLMDLGGEYYSLYT
                     KRK"
BASE COUNT          931 a          550 c          509 g          962 t
ORIGIN      
        1 cacactcctt tttataaacc tgaaataagg catcaattgt aacaaattct cctgcctctg
       61 tagccttcaa atgacgggca agaaaggctt tctcttcctc aactgtcata tatgcatggt
      121 tacgaccacc acgtgtttct tgaaggagag agtcgagtcc gaactcctca tattttttta
      181 cgtttcgcca aatcgttgtt tgattacagt ctaaaagctc tataatctct ttataagatt
      241 tgcccatcag acgaaatata gtagattgaa actagaatag tacacctcta cttctaaaac
      301 attgttagaa atcgatttgt cctgttcttg tttcatttta ctatagaacg atttgaaggc
      361 gtttataata tttagctgta cgagagtctt ttaaaagtgt tttgatggtt tggatttctt
      421 ctttagttga tttcatatta ctattatata atgctttttg attttagtct ggtataaata
      481 ttgctttcct ccaaaatggt catagtttta ctggcaaatc taacatatca cggataaatt
      541 aacaagtgat ttctgaattg ctaaacattt tcttttctta tagcatactt taagattttg
      601 tctttgagaa agatatttcc aagaaaaacg ttcgtttttt tatctatttt caaaaaaacc
      661 aaaaaactga attgaaagaa tttttagaaa atttctgttt tttctttaca gtgaaaaaaa
      721 attctgctat aatgtcttat gtaataaaat atgacaataa aaggagaacg atatgacatc
      781 tgctaaagaa tatatccaaa gcgtgtttga aactgtgaaa gctcgtaacg ggcacgaggc
      841 tgaattcctc caagctgttg aagaattttt caacacttta gaacctgtat ttgaaaaaca
      901 ccctgagtat atcgaagaaa atatcttggc acgtattact gagcctgagc gcgtggtttc
      961 tttccgtgtt ccttgggttg accgtgatgg aaaaattcaa gtaaaccgtg gttaccgtgt
     1021 tcaattcaac tcagctgttg gaccatacaa aggtggactt cgtttccacc caactgtaaa
     1081 ccaagggatc ttgaaattcc tcggatttga acaaatcttt aaaaatgtct tgactggact
     1141 tcctatcggt ggaggtaaag gtggatcaga cttcgatcct aaaggtaaaa cagatgctga
     1201 agtgatgcgc ttctgccaaa gcttcatgac tgaattgcaa aaacacatcg gaccatcact
     1261 tgacgtacct gctggtgata tcggtgttgg tggacgtgaa attggttacc tttacggtca
     1321 atacaaacgt cttaaccaat ttgatgctgg tgtcttgact ggtaaacctc ttggatttgg
     1381 tggtagcttg attcgtccag aagcaactgg ttacggtttg gtttactata ctgaagaaat
     1441 gctcaaagct aacggtaaca gctttgctgg taagaaagtc gttatttcag gttctggtaa
     1501 cgttgctcaa tatgctcttc aaaaagcaac tgaactcggt gcaactgtta tttctgtttc
     1561 tgactcaaat ggttatgtca tcgatgaaaa tggtatcgac ttcgatcttt tggttgatgt
     1621 taaagaaaaa cgtcgtgctc gtttgactga gtatgcagct gaaaaagcaa ctgcaaccta
     1681 ccacgaaggt actgtatgga cttacgctgg aaactatgac attgctcttc catgtgccac
     1741 tcaaaacgaa atcaacggtg aagcagctaa acgtttggtt gctcaaggcg ttatctgtgt
     1801 atctgaaggt gccaacatgc cgagcgacct tgatgccatc aaagtttaca aagaaaatgg
     1861 tatcttctac ggacctgcaa aagctgccaa cgctggtggt gtagccgttt cagctcttga
     1921 aatgagccaa aacagtcttc gcctctcatg gactcgtgaa gaagttgatg gccgtctcaa
     1981 agacatcatg acaaacatct tcaacacagc taaaacgact tcagaaacat acggtcttga
     2041 taaagactac cttgcaggag ctaacattgc tgcctttgaa aatgtagcaa atgccatgat
     2101 tgcacaaggt attgtttaag atttatttac tcaatcctca atcactacga ttggggattt
     2161 ttagcttgaa ttaaaaaatt ggaaaatcta attaaataaa atgaaatgaa ataaaaactg
     2221 aacaaattga ttgcggaatt caaaccaatt tctaacatat ttctagaaat taatttgact
     2281 tttctaatcg atttgttcat atcttatttc aatttactat acttacttga ccctttttat
     2341 actcaatgaa aatcaaagag caaactagga agcgagccgc aggctgtact tgagtacggt
     2401 aaagcaaagc tgacgtggtt tgaagagatt ttcaaagagt attaaacaaa aagaaaaacc
     2461 gctactccta agaatagcga tttaatcatg tttttaagct actactgtag ttgctctatt
     2521 atttaagtgt tatgaataac ttcaatatct ttaaagcttt ttgttagatt tataatttca
     2581 actatagcca tctctactcc ttagtttaca gcaaaaaatt atgcttaatt cttattgttt
     2641 ttgtaaacaa tcagcgaacc caatactatt ccattcaaca agcctaaaga aataccatat
     2701 tcaactggaa aatctaaaaa aattgcaaac actattccaa tcatcactcc tagtgaggct
     2761 gttgttaaaa ttacatattc attttctttc ttcattttct ttacacctca tttccttttt
     2821 gtatataagc tgtagtactc tccaccaaga tccattaagt acttatgatt tcctgactca
     2881 acaatcttac cacctttcat tacaaaaata acatccgcat ccttaatcgt tgacaatcta
     2941 tgagctacaa tt
//
LOCUS       DBERYS010000087         2849 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.087, whole genome shotgun sequence.
ACCESSION   DBERYS010000087 DBERYS010000000
VERSION     DBERYS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.087"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(4..945)
                     /locus_tag="ACL8LA_002002"
     CDS             complement(4..945)
                     /locus_tag="ACL8LA_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS1813695.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(923..2686)
                     /gene="aspS"
                     /locus_tag="ACL8LA_002003"
     CDS             complement(923..2686)
                     /gene="aspS"
                     /locus_tag="ACL8LA_002003"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGS1813696.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          823 a          679 c          567 g          780 t
ORIGIN      
        1 tcactaatta tctatctcaa caaatcttcc tagaatatga acattttccg agacagagac
       61 aaaggagctt ggatccactt gtgtcataat ctgtttaaat tcattaaact ctgcacgtgt
      121 aatgacagtg attaaaactg cctttctctc gtgattatag gttccttctg catcgtggat
      181 catggttgct ccgcggtgca attttttatg gattttttca attaccttct ctggatgatt
      241 tgtcacaatc atggcctgca tacgcttttg cttagtaaag actgcgtctg tcacacggct
      301 agagacaaag atggtaatca tagaataaag agcgtatttc caaccaaagg tcaaacctgc
      361 tatcagcatg atagttccat ttaccaagaa agaaatacta ccgacattct tacccgtttt
      421 cttgcgaata gtcaggctga cgatatccgt cccaccactg gagatattgt ttcgaagagc
      481 aaaaccaatc cccaaaccca taacaacacc cccaaaaagg gaattgataa tgggatcctc
      541 cgtcaaggtt gccacaggga caaactggat aaagaaggaa ctcatagata ccgtgataaa
      601 ggtaaagacg gtgaacttat ggccaatctg ataccaagct aagaccatca aagggaagtt
      661 aatggcgtag aagcttagcg aaatcggaat atgaaaacca aaccagtgat tactcaaggc
      721 agagataatc tgtgccagac ctgttgcacc actcgaatac acatgccctg gttggaaaaa
      781 gaaattaact gctactgctg ataaaaaacc atagaccaga gaggccgaaa tcttctcatc
      841 atacttttct cgagagatac tttgtaagac acgtaaaatt tttatctgat aagcaaagcg
      901 gcgcagataa tagcgccacc gcttaattcg ttttgtttgt ttcatcttct tctacttgta
      961 agctgagttc ctctagttgt ttgagagcga ctgttgatgg agcttgtgtc attgggtcag
     1021 ttgccttgtt gttcttagga aaggcaatga cttcacggat attttcttct ccagcaagca
     1081 acatgacaaa acggtcaagc ccgatagcca aaccaccgtg tggtgggaaa ccatagtcca
     1141 tggcttcaag aaggaaacca aactggtcat tggcttcttc agttgagaaa ccaagagcct
     1201 tgaacatgcg ttcttgaagg tctttttggt tgatacgaag gctaccacca ccaagctcat
     1261 aaccgttcaa gacgatatcg taagcaatgg cacgaacctt agccaaatca ccttctaatt
     1321 cgtgagcagt ctcttcctgt ggaagtgtga atggatggtg ggcgctcatg tagcggcctt
     1381 cttcttcaga ccattcaaac atcggccagt caaccaccca aaggaagttg aacttatcat
     1441 tatcaatcaa gccaagctct ttagcaatac gtccacgaag ggcacccagt gttgcattag
     1501 ccacttcaag cgtatccgcc acaaagagaa ccaagtcctt atcttcaaga gcaagcgctg
     1561 ttgtcaattc ttcttggata ccagtcaaga acttggcaac tggtccgttt aattctccat
     1621 caaccacctt gacccaagca agacctttgg caccatactg tttggctact tccgtcatct
     1681 tgtcgatgtc tttacgtgaa tagttgtccg cagctccttt gaccacaatc gcttttacag
     1741 caggtgcttc tgaaaagact ttaaagtcta cacctttgac cacttctgtc aagtcctgaa
     1801 gcaacatgtc aaaacgagta tctggcttgt cagaaccgta aagagccata gcatcatcgt
     1861 atttcatacg agggaacggt agcgttactt cgatgccttt tgtttccttc atcacgcgcg
     1921 cgatcaagcc ttctgtaata tcttggattt cttgctcagt aaggaaggac gtttccaagt
     1981 cgacctgagt aaattcaggc tggcggtctc cacgcaagtc ctcgtcacgg aaacatttaa
     2041 cgatttggta gtaacggtca aaaccagcat tcatcaagag ctgtttcgtg atttgtggac
     2101 tttgaggaag agcgtaaaaa tgccccttat taacacgaga cggcactaaa taatcacgcg
     2161 ccccttcagg cgttgactta gaaaggaatg gtgtctccac gtcgataaac tccaactcat
     2221 ccaagtagtt gcggatagag tgggtcacct tggcacgaag tttaagattt tccaacattt
     2281 ctggacgacg aaggtcaagg taacggtaac gcaaacgtgt atcgtcattt gcctcaatgc
     2341 catccttaat ctcaaatggt gttgtcttag ctgtgttaag cacaataaga gctgtcacgt
     2401 ttaactcaac cgcaccagtt ggcaacttat cattggcttg ctcacgcgca gcgacctgac
     2461 cagtcacctc aataacaaat tcgctacgaa ggctttcagc tgttgccata acctctgcag
     2521 atactttttc agggttgata accaactgca tgattccttc acggtcacga agatcgataa
     2581 agatcaaacc accaaggtca cgacgacggc caacccatcc tttcaaggtt atttcttgtc
     2641 cgatgtgttc ctcacgaaca cgaccagcat acatactacg tttcattatt tctctcctct
     2701 tttattctgt tactatttta ccataaaagc gcaggctctt catgaaaatc atcagaaaag
     2761 tttgccagtc tttaaaagtc aggtgaaagc cctaaaaatt agcgctaata ctcttcgaaa
     2821 atctcttcaa accacgtcag cgtcgcctt
//
LOCUS       DBERYS010000088         2797 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.088, whole genome shotgun sequence.
ACCESSION   DBERYS010000088 DBERYS010000000
VERSION     DBERYS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.088"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..821
                     /locus_tag="ACL8LA_002004"
     CDS             <1..821
                     /locus_tag="ACL8LA_002004"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1813697.1"
                     /translation="EKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPND
                     ESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRL
                     NDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRD
                     GDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPI
                     VQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            878..1675
                     /gene="psrA"
                     /locus_tag="ACL8LA_002005"
     CDS             878..1675
                     /gene="psrA"
                     /locus_tag="ACL8LA_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HGS1813698.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1686..2294
                     /locus_tag="ACL8LA_002006"
     CDS             1686..2294
                     /locus_tag="ACL8LA_002006"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1813699.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(2242..>2797)
                     /locus_tag="ACL8LA_002007"
     CDS             complement(2242..>2797)
                     /locus_tag="ACL8LA_002007"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1813700.1"
                     /translation="NIPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIK
                     PLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGG
                     FIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLA
                     PFEEQELITQKVEKLFEKVNQLWK"
BASE COUNT         1010 a          409 c          466 g          912 t
ORIGIN      
        1 tagaaaaagt agatgaatat gctgaaagtt ataatagact agaacagcta gataaagaat
       61 ttccagataa actaaaaaaa tctattcttc aatatgctat gcaaggaaaa ttagttgaac
      121 aagacccaaa tgatgaatca gtcgaagttt tacttgaaaa aatacgagca gaaaaacaaa
      181 aactctttga agaaggcaag attaaaaaga aagatttgga catttctatt gtttcccaag
      241 gagatgataa ctcttattat gaggaagtac cttgtgaaat accagaaagt tgggaatggg
      301 tgaggttaaa cgatataacc tcctacattc aaagagggaa gtctccaaaa tattcaaata
      361 ttcctatata ccctgtgatt gctcagaaat gtaatcaatg gtctggcttt tctattgact
      421 tagcaagatt tattgatccc gaaacagttc atagttacca gaaagaaaga ttactaagag
      481 atggtgattt aatgtggaat tcaacaggtc taggtacgtt aggacgatta gctatttacc
      541 atgaaaataa aaatccttat ggctgggctg ttgcagatag ccatgttaca gttataagag
      601 tcttatcggg agttattaat tgccatttca tctataattt tctttcctct cctattgttc
      661 agtcagttat tgaagaaaaa gcgtcgggta gtacaaaaca aaaagaatta ttaacgaaaa
      721 ctataaaaga atatttaatc cccctcccac cccttcctga acaatccaga atcgttgata
      781 aaatcgaaca atttttcgcc catattgacg cactaattta gtataatagt tttacgtgcg
      841 gtggaatttc tatcaaacat aagataggag ttttacgttg aaagattttg aattacattt
      901 gaagaaagct ggtttagctg aaaatactgt gcgttcgtac ctttatggtg ttcgattttt
      961 cttggagaat tacgaactca aaatggaaga tttatttgaa tacaaaggat atttattaga
     1021 caattttaaa cctaaaactg tcaatttgcg actacaagga gtaaataaat atctggcttt
     1081 tattggtcat gatgatttga aattaaaatt tgtcaaagta cagcaaaaac catttttaga
     1141 agatgtcatt agccatgctg attacctttt tcttaaacgt agtttgaaaa aagatggtat
     1201 tttaaaatgg cattttgtcg tttggttttt aggtgcaact ggggctcgtg taagtgaact
     1261 tattaaacta aaagtagagc atgtagaaat cggttatttt gacatttact caaaaggtgg
     1321 taaaattcgt agactgtaca ttcctaaaaa attaagaaat agttgtctta gttggttaga
     1381 atcagaaaat cgccgaagtg gttatttatt tttgaataaa tttaacgaac caattacagc
     1441 aagaggagtt gctcaacagt taaaaaatta tgctgataaa tacaaaatga atcctaaagt
     1501 aatttaccct cattctttta ggcatttatt tgctaagaat tttttagcga agtataatga
     1561 tattgccttg cttgcagatt tgatgggaca cgaaagtata gaaactactc gaatttatct
     1621 aaggaaaaca gctactgaac aacaaaatat tgtagataaa attgttaatt ggtaaaaaat
     1681 aacaggtggt caaactgact acctgctatt tttgtgatta tggctcttat tatgggaaca
     1741 aagacgaaac aacctcttat cctatatatg aaatcccaga agcatggaga tatattaaat
     1801 ttgctagcct agttaacttc agaataggaa aaactcctcc acgcagtgaa gctacttttt
     1861 ggggaactga aataccttgg gtatctatat cagatatgcc tatatctggt tatgtaacta
     1921 atacgagaga atctatttct aaattagcat taaaatcaaa gaaaatagac atttcaccta
     1981 aaggaacttt attaatgagt tttaaattat ctattggaaa agttgccata ttggatattc
     2041 ctgccactca taatgaagcc attatatcga tattccccta tgctaataaa gaaaatatta
     2101 tcagagatta cttaatgata tttttgccac ttatctctac tttaggtgat tcaaaagatg
     2161 ctataaaagg gaaaacatta aatagtacca gtatctccga attattaatc cctatttcta
     2221 atcatgaaga gatgaaaaga atcatttcca aagttgattt actttttcaa aaagtttctc
     2281 aactttttga gtaataagtt cctgttcctc aaaaggagct aacggaatta atagctcgct
     2341 tagtgtagtt ttaggaatat tatataaagc ttgacctgat agtttagtta ttgctttcaa
     2401 ttgtttataa aataacggag aggacaagtt aaataataga aattttgaaa taatctctga
     2461 actttcgaat ggtgttaatt ggaaaataaa tccaccagcc acaacaccat catagtcttt
     2521 atcgattctt gcaaactttc caatatgttc taaagaggtt gatacaggtg ttattagctg
     2581 attatgtttt aaataaactt gttcagagga gatgaattgt gtatcaatgt agtaatcatt
     2641 atccaacaga gaaaattcta aaggcttaat attaccacca cgtataattc taacaccttt
     2701 attattaatg cttaaatcgc ccttcttgta agaaagacct gtattcattg aaaaaatatc
     2761 ttttattttt ataacaaccc aattcatagg tatattc
//
LOCUS       DBERYS010000089         2589 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.089, whole genome shotgun sequence.
ACCESSION   DBERYS010000089 DBERYS010000000
VERSION     DBERYS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.089"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            136..267
                     /locus_tag="ACL8LA_002008"
     CDS             136..267
                     /locus_tag="ACL8LA_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGS1813701.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKVKAEKKN"
     gene            268..1173
                     /locus_tag="ACL8LA_002009"
     CDS             268..1173
                     /locus_tag="ACL8LA_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGS1813702.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            1170..1985
                     /locus_tag="ACL8LA_002010"
     CDS             1170..1985
                     /locus_tag="ACL8LA_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGS1813703.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2115..>2589)
                     /locus_tag="ACL8LA_002011"
     CDS             complement(2115..>2589)
                     /locus_tag="ACL8LA_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1813704.1"
                     /translation="LILLDGGYLDLDKILPLDTELEETKNYIKSQIISDLDVLTSKEK
                     SEAKHWSENMEKAVRQSYHWNVEYNRYELAINYENIEAILRLRRKIQAFKREVGDTLF
                     ISPRYPNEATWREEALKELPDYFDTIFLENFGHELYTQAPKEIASLMNEWLAYFL"
BASE COUNT          739 a          546 c          460 g          844 t
ORIGIN      
        1 tactacggta aggcgaagct gacgtggttt gaagagattt tcgaagagta tcaactatcg
       61 aaaacaaatt aaaaaactcc cttttccgca aaataatagt ataatagagg tagaatttag
      121 aatcgaggta cacctatggc tgtcaaattt acaaaacgag acgacttgga caagatgttt
      181 gaagagtttg ctaaactccc tgatttgaaa caagttactt tccctgatga caaagagaaa
      241 aaagtcaaag cagaaaagaa aaactagatg actgcttttc aacaactccc atctagtgta
      301 cttcaaactg gagccatttt tctctccatt atcattgaag cccttccctt cgttctgata
      361 ggaagcattg tctcagggct gattgaagtt tatatcacac ctgacaaggt ttatcatttt
      421 ctccctcgaa atcgttgggg gagaatcttt tttgggacct ttgtcggtat acttttccct
      481 tcttgtgaat gtggaatcgt ccccatcatc aatcgttttc tggaaaaaaa ggttccaagt
      541 tacacggccg ttccttttct tgtgacagca cctgttatca atcccattgt tctttttgcg
      601 acctattctg cctttggcaa ctccttccat gtcgccctat tacgagctct gggttccatt
      661 cttgtggctg taatactagg aatttttcta ggatttttct ggcaagaacc gattcagaaa
      721 gaaaatcgtc tggcttgtca tgagcatgat ttttcttact tgagttctgc aaaaaaagtt
      781 tttcaagtct ttgtgcaggc cattgatgaa ttttttgata cggggcgtta tttggtattt
      841 ggctgcctct ttgcttctat aatacaggtc tacgttccga ctcggattct gacctctatc
      901 agtgcgaccc ctctttttgc catcctgctc ttgatgattt tagcctttct tctttcgctc
      961 tgtagtgagg cggatgcctt tataggtgct tctcttctct cgagtttcgg tttggcacca
     1021 gttctggcct ttctcgtcat tggtccaatg ctggatatca aaaatattct catgatgaaa
     1081 aattacttga aagcacgatt tatcagtcac ttcataacaa ttgtaactct tgtcgtctta
     1141 gtctattctc tcttgattgg agtcatccta tgattcgatt tttagtttta gctggctatt
     1201 ttgaactgac tatttacctc catctgtcgg gcaaactaaa ccagtacatc aacatgcact
     1261 attcctatct ggcctatatc tccatggtgc tttcttttat cttggctatc gttcaattgt
     1321 atatctggat gaagcaagtc aaaacccaca gtcatctgaa cagccgattg gccaagatga
     1381 cgagtatttc tcttctggct attccacttg tcatcggctt aactttccca actgttagct
     1441 tggattctca gactgtttca gctaaaggct atcatttccc tctatcggaa ggaacgaatc
     1501 tggctattca gacaagtgaa gggactacaa gccaatattt gaaaccagat actagttctt
     1561 atttttcaaa atcagcctat gaaaaggaaa tgcgaacggc ggcggataaa tacttatccc
     1621 aagacagcat tcagatcact aatgaaaact atatggaagt catggaggct atctacgact
     1681 atccagatga gtttgagggc aagacaatcc agtttacagg ctttgtctat aacgacccca
     1741 gtcatgccaa tagtcaattt ctgttccgat tcggcattat ccactgtatc gcagattctg
     1801 gtgtctatgg attgctgacc aagggcaata cccggcagta tgaaaacaac acttggattt
     1861 cagccaaagg aaaactggtc aatcactacc ataaagaact caaacaaaac cttccaacct
     1921 tggaaatcga cagctttacc aaagtcgata aaccagaaaa tccctatgta tatagagctt
     1981 tttaagaaaa tcaagataaa aacgaacaag ttctcttctg aataacagaa aaagagcctg
     2041 ttcgtttttt ggttatatga aaattagtga cttgtagatt ttcatcttat accatttcca
     2101 gcaatacaag tagctcatag aaaataagcg agccactcat tcattagact agcgatttct
     2161 ttaggtgctt gagtataaag ctcatggcca aagttttcta aaaaaatagt atcaaaatag
     2221 tctggcaatt cttttagggc ttcctctctc catgtagctt cattaggata gcgaggacta
     2281 ataaacaagg tatctcccac ttctctctta aaagcttgta tttttctccg taggcggagt
     2341 atcgcttcta tattttcata atttatagcc aactcatatc tattatactc aacattccag
     2401 tgataggact gtcttacagc tttctccata ttttctgacc aatgctttgc ttcagatttt
     2461 tctttagaag taagaacatc taagtccgaa ataatttgag atttgatata atttttagtt
     2521 tcctctaact ctgtatccaa aggtaaaatc ttatctaaat ctagatagcc accatccaaa
     2581 agaatcagt
//
LOCUS       DBERYS010000090         2540 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.090, whole genome shotgun sequence.
ACCESSION   DBERYS010000090 DBERYS010000000
VERSION     DBERYS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.090"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(208..873)
                     /locus_tag="ACL8LA_002012"
     CDS             complement(208..873)
                     /locus_tag="ACL8LA_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGS1813705.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(887..2266)
                     /locus_tag="ACL8LA_002013"
     CDS             complement(887..2266)
                     /locus_tag="ACL8LA_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS1813706.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
BASE COUNT          834 a          579 c          469 g          658 t
ORIGIN      
        1 ttctatccac aacctcaaaa cagtgttttg agcagcccgc agctagtttc ctagtttgat
       61 ctttgatttt cattgagtat tactctctct tgtcacttcc ttctatttta ccataaagtc
      121 cagcctttga agaactttta ctagaagaca aggggcttct gtctctattt gccatcttaa
      181 gcatcaaaaa agaggggtca tccctcttta cgaattcaat gctactaggg tatccaaata
      241 ctggttgttg atgactgcca aaatataggt atctgctttc aagaggtcat ctggtccaaa
      301 ttcaacatcc aatggggaat tttcctgctc tcggaaaccc aaaatattca gattgtattt
      361 gccacggagg tctaatttac tcagactttg acctgcccaa gactgaggaa ttttcatctc
      421 cacgatagac acatttttat ccaactgaaa gacatcaaca ctattatgga aaagaatggt
      481 ctgtgctaga gactgcccca tttcatactc tggcgagata accgagtcag ctccaatctt
      541 ttctagcact ttcttagcgg tctgactttt gaccttagca ataacagtcg gtacccccaa
      601 actcttacag tgcataaccg caagcacact cgactccaga ttttcacctg tcgcgactac
      661 aacggtatcg caggtatcaa tccctgctga tctcaataat tcttcatctg tgatgtcacc
      721 aatcactcca cgcgccaaaa ctggctcaaa ctgattgatg cgctctgcgt ggtcatcaat
      781 agcgataata ttcatatcct gcttggctag ggcagctagg acactgctcc caaaaattcc
      841 caagcccaaa attccaatcg tacgatctga catgctcttt cctttcttaa ccaatactaa
      901 tatctgcttt catatagtga atcatatctt tcttttctgg atggtaatct gccaagctaa
      961 caaacaaggt caagggacca attcgtccca taaacataag tggcatgata acactgagag
     1021 ccaatttccc aaggtcagga gtcagatttg ccgttacacc aactgtacta agagctgaaa
     1081 tggtttcaaa tatgaggtgg ataaagggag gattgccttt ggctgttatc cctagcagaa
     1141 tcaatcctat caagaagctc atcaaaaaga taataaagac actaaaggat ttttgaaccg
     1201 ttcgcggcgc gatcgttcgt ctcgcaacat tggcatgagg caagcctaga agctcacttc
     1261 gtgcaaagac caagaggaca aaaaatgtcg taatcttgag tcccccagct gttcctccag
     1321 gtgccccacc tagaaacatc tgtaagatat aaatcaaaag agtcacagga tgagcctgag
     1381 tataatctat cgtagaaaag ccagctgttc gcatcgtcac tgtttgaaaa aagctaacta
     1441 aaaccttatc ggcaacaggg agattgccaa tcgttccagc attgttccac tcaagaaaga
     1501 gagtagttgc cgttccaaat aacaacaaac ctatagtcaa taataataca agcttcgtat
     1561 gaaagtgcag acgtcctttt ttctttcttc ctacatgacc agccaaatca aaccagacca
     1621 taaaaccaag gccgcctgta ataatcaagc ctgcaatcac cagattgacc agtaaatcgg
     1681 tctgaaaagc aaataaactg gtgctcccta aattatcaaa accggcatta cagaaggctg
     1741 agatcgctag aaaaatggaa ctaaaaagac cacgtcccca gccaagttga ggaataaggc
     1801 gaaaactaag caaaatagct cccaagctct caaccaaaaa ggtcgtgaga aaaatagaat
     1861 agacaaactt tctcaaagat cgagtttctc cataactaaa actatcctga atagttgcac
     1921 ggctacgaag actaagcttt tgcttgctct ggatatagaa aaccccaata aaggtcatga
     1981 gccctagacc accgatctga atcaagagca aacagattat ttggccccag atattatagg
     2041 tgtgagctac tggaagggtt gagagacctg tcacacagac tgcagagaca gcagtgaaaa
     2101 gatgatcaaa ataagtcgct cgtgagcttt caacttggac aaagggcaag ctcaaaagaa
     2161 gagagcctag taaaatgacc aaggcaaaac tcaaaaaaat acgacgggct ggcgataaac
     2221 gagataagcc tagctctatt ttttccttag ataatttgaa taacatgttt ccattgtacc
     2281 ataaaaagct cgaaatgact tagataggat aaggcaaaat tcactaacac caagcctaaa
     2341 aaatgccacc tttttattga tagcttaggc aagaacaata cttgatagat atgactatag
     2401 gtcaaaaaag caagacagga gcttttaacg ccagctactc tatatgcttt tcaggagaaa
     2461 atttctcttt ttgattatag gcaatgaaaa ccaaggcatc gattagaaag ctagtcacag
     2521 gttgctcaaa acactgtttt
//
LOCUS       DBERYS010000091         2253 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.091, whole genome shotgun sequence.
ACCESSION   DBERYS010000091 DBERYS010000000
VERSION     DBERYS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.091"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1545
                     /gene="phtA"
                     /locus_tag="ACL8LA_002014"
     CDS             <1..1545
                     /gene="phtA"
                     /locus_tag="ACL8LA_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGS1813707.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGDHYHFIPYSQMSELEER
                     IARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVG
                     EGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDK
                     AYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPER
                     LGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGK
                     DSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPY
                     MVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDE
                     RPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLK
                     EAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSV
                     SKEKIN"
     gene            1703..>2253
                     /locus_tag="ACL8LA_002015"
     CDS             1703..>2253
                     /locus_tag="ACL8LA_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS1813708.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYT"
BASE COUNT          802 a          406 c          456 g          589 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aacagctaga
       61 ggtgttgcag tgccacacgg agatcattac cacttcatcc cttactctca aatgtctgaa
      121 ttggaagaac gaatcgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaaggc cagaacaacc aagtccacaa ccgactccgg aacctagtcc aggcccgcaa
      241 cctgcaccaa atcttaaaat agactcaaat tcttctttgg ttagtcagct ggtacgaaaa
      301 gttggggaag gatatgtatt cgaagaaaag ggcatctctc gttatgtctt tgcgaaagat
      361 ttaccatctg aaactgttaa aaatcttgaa agtaagttat caaaacaaga gagtgtttca
      421 cacactttaa ctgctaaaaa agaaaatgtt gctcctcgtg accaagaatt ttatgataaa
      481 gcatataatc tgttaactga ggctcataaa gccttgtttg aaaataaggg tcgtaattct
      541 gatttccaag ccttagacaa attattagaa cgcttgaatg atgaatcgac taataaagaa
      601 aaattggtag atgatttatt ggcattccta gcaccaatta cccatccaga gcgacttggc
      661 aagccaaatt ctcaaattga gtatactgaa gacgaagttc gtattgctca attagctgat
      721 aagtatacaa cgtcagatgg ttacattttt gatgaacatg atataatcag tgatgaagga
      781 gatgcatatg taacgcctca tatgggccat agtcactgga ttggaaaaga tagcctttct
      841 gataaggaaa aagttgcagc tcaagcctat actaaagaaa aaggtatcct acctccatct
      901 ccagacgcag atgttaaagc aaatccaact ggagatagtg cagcagctat ttacaatcgt
      961 gtgaaagggg aaaaacgaat tccactcgtt cgacttccat atatggttga gcatacagtt
     1021 gaggttaaaa acggtaattt gattattcct cataaggatc attaccataa tattaaattt
     1081 gcttggtttg atgatcacac atacaaagct ccaaatggct ataccttgga agatttgttt
     1141 gcgacgatta agtactacgt agaacaccct gacgaacgtc cacattctaa tgatggatgg
     1201 ggcaatgcca gtgagcatgt gttaggcaag aaagaccaca gtgaagatcc aaataagaac
     1261 ttcaaagcgg atgaagagcc agtagaggaa acacctgctg agccagaagt ccctcaagta
     1321 gagactgaaa aagtagaagc ccaactcaaa gaagcagaag ttttgcttgc gaaagtaacg
     1381 gattctagtc tgaaagccaa tgcaacagaa actctagctg gtttacgaaa taatttgact
     1441 cttcaaatta tggataacaa tagtatcatg gcagaagcag aaaaattact tgcgttgtta
     1501 aaaggaagta atccttcatc tgtaagtaag gaaaaaataa actaatgaaa aatgaaagtc
     1561 tcgataaaga ggctttcatt tttattatgt atatatgtaa aattcttgac aagcaatatt
     1621 aaaaagagta aactattaac tagttaatta accggtttat tactttatag tgaatcaaat
     1681 atacttaaga aaagaggaaa gaatgaaaat caataaaaaa tatctagctg ggtcagtagc
     1741 tacacttgtt ttaagtgtct gtgcttatga actaggtttg catcaagctc aaactgtaaa
     1801 agaaaataat cgtgtttcct atatagatgg aaaacaagcg acgcaaaaaa cggagaattt
     1861 gactcctgat gaggttagca agcgtgaagg aatcaatgcc gaacaaatcg tcatcaagat
     1921 aacagaccaa ggctatgtca cttcacatgg ggaccactat cattattaca atggtaaggt
     1981 tccttatgac gctatcatca gtgaagaatt gctgatgaag gatccaaact accagctaaa
     2041 ggacgaggat attatcagtg aaatcaaggg tggttatgtg attaaggtag atggaaaata
     2101 ctatgtttac cttaaggatg cagctcatgc ggataatgtc cgtacaaaag aagaaatcaa
     2161 tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg acttcaacaa acgatggtgc
     2221 ggtagccttt gcacgttcac aaggacgcta tac
//
LOCUS       DBERYS010000092         2201 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.092, whole genome shotgun sequence.
ACCESSION   DBERYS010000092 DBERYS010000000
VERSION     DBERYS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.092"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..271)
                     /locus_tag="ACL8LA_002016"
     CDS             complement(<1..271)
                     /locus_tag="ACL8LA_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813709.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTDYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADF"
     gene            complement(216..398)
                     /locus_tag="ACL8LA_002017"
     CDS             complement(216..398)
                     /locus_tag="ACL8LA_002017"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1813710.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            733..>2201
                     /gene="pcpA"
                     /locus_tag="ACL8LA_002018"
     CDS             733..>2201
                     /gene="pcpA"
                     /locus_tag="ACL8LA_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGS1813711.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKG"
BASE COUNT          788 a          312 c          361 g          740 t
ORIGIN      
        1 caaaatcagc cgcaatttct tcataagtgc ggtattctcg cacatattga agagtggcca
       61 taagaaggtc ttctaggctt aatttaggtt ttcgtccacc ttttgcgtgt ttaagttgat
      121 aatctgtttt taacacagct aacatctctt taaaagtggt acgctgaaca ccaacaagac
      181 gcttaaatcg tgtatcagtt aattgtttac ttgcttcata atttcgcagg gagtctattg
      241 actctttggt aggtgtcaat gtttttttca tctataccga gaattatttt cccgccattt
      301 gtatttgcaa atgctgagta ggtttcccag aaagactctg gaagattgtt tttagctttt
      361 ttgtattcta aatcaacccc ttcaaatttt aagtccatat ttttccttta catctgtttt
      421 ttgtggttct ggtatttgtt caagttgagt gataatatag cgaattgaat ttcgagagtt
      481 tttactcagt taatttcttt tttaacccgc tttaatttct tttttaacac gtgttaaaaa
      541 agaaattaaa gcgggttaat ttttcttgac ttaaaattta aaaatgatat actgtgttta
      601 tggagagatg acaatttata aaatgtacta ctctattcta ctagataatg attgaataaa
      661 actttttata acaacggcta gcaaagttat aattttatat ctattagaaa ataaatatgt
      721 aaggaaattt aaatgaaaaa aactacaata ttatcattaa ctacagctgc agttatttta
      781 gcagcatatg tccctaatga accaatccta gcagatactc ctagttcgga agtaatcaaa
      841 gagactaaag ttggaagtat tattcaacaa aataatatca aatataaggt tctaactgta
      901 gaaggtaaca taggcactgt tcaagtgggt aatggagtta ctcctgtaga gtttgaagct
      961 ggtcaagatg gaaaaccatt cacgattcct acaaaaatca cagtaggtga taaagtattt
     1021 accgttactg aagtagctag tcaagctttt agttattatc cagatgaaac aggtagaatt
     1081 gtctactatc ctagctctat tactatccca tcaagcataa aaaaaataca aaaaaaaggc
     1141 ttccatggaa gtaaagctaa aactattatt tttgacaaag gtagtcagct ggagaaaatt
     1201 gaagatagag cttttgattt ttctgaatta gaagagattg aattgcctgc atctctagaa
     1261 tatattggaa caagtgcatt ttcttttagt caaaaattga aaaagctaac cttttcctca
     1321 agttcaaaat tagaattaat atcacatgag gcttttgcta atttatcaaa tttagagaaa
     1381 ctaacattac caaaatcggt taaaacatta ggaagtaatc tatttagact cactactagc
     1441 ttaaaacatg ttgatgttga agaaggaaat gaatcgtttg cctcagttga tggtgttttg
     1501 ttttcaaaag ataaaaccca attaatttat tatccaagtc aaaaaaatga cgaaagttat
     1561 aaaacgccta aggagacaaa agaacttgca tcatattcgt ttaataaaaa ttcttacttg
     1621 aaaaaactcg aattgaatga aggtttagaa aaaattggta cttttgcatt tgcagatgcg
     1681 attaaacttg aagaaattag cttaccaaat agtttagaaa ctattgaacg tttagccttt
     1741 tacggtaatt tagaattaaa agaacttata ttaccagata atgttaaaaa ttttggtaaa
     1801 cacgttatga acggtttacc aaaattaaaa agtttaacaa ttggtaataa tatcaactca
     1861 ttgccgtcct tcttcctaag tggcgtctta gattcattaa aggaaattca tattaagaat
     1921 aaaagtacag agttttctgt gaaaaaagat acatttgcaa ttcctgaaac tgttaagttc
     1981 tatgtaacat cagaacatat aaaagatgtt cttaaatcaa atttatctac tagtaatgat
     2041 atcattgttg aaaaagtaga taatataaaa caagaaactg atgtagctaa acctaaaaag
     2101 aattctaatc agggagtagt tggttgggtt aaagacaaag gtttatggta ttacttaaac
     2161 gaatcaggtt caatggctac tggttgggtt aaagacaaag g
//
LOCUS       DBERYS010000093         2076 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.093, whole genome shotgun sequence.
ACCESSION   DBERYS010000093 DBERYS010000000
VERSION     DBERYS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.093"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1445)
                     /locus_tag="ACL8LA_002019"
     CDS             complement(<1..1445)
                     /locus_tag="ACL8LA_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGS1813712.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVI"
BASE COUNT          606 a          466 c          383 g          621 t
ORIGIN      
        1 ttaataacgt tgatatgatt gacctctgga tcttccgtcc cttggaggga gcaaccacgt
       61 tccttggcga attggatata gtctacaatt tctcgtgtaa tgcgttcacc aggagccaag
      121 ataggaatcc ctggaggata gcacatgaca aattctccac agacctgtcc aacagactca
      181 tctaaggtca aactttttct ctctgaatag aaggcttctt gtggagacag caccaactcg
      241 ggctgaatat attctcctgc tatcaaatct tttccatctc ttgaatagag tctcttaata
      301 tcagccagag caccaaccaa gcgctcgatg tcttggatgc ggtcgccgat ggaaatatag
      361 gccaagatat tgccgatatc accaaactcg atctgaatgt cgtattcgtc tcgcaagagg
      421 tcataaacct cgatacctgt taagccaata ccctgagtgt aaactgacag cttagttacg
      481 tcaaaatcgc aaaccgaaac accgtctatt aactcttttg agtaggcata gtagccaccg
      541 atagcattga tttcacggcg ggcatactca gatagctcaa tgactttctc aaacgactct
      601 ttaccacgaa gggccaagtt gcgacgtgaa atatccaaac tagccatcaa caagtaagag
      661 gcagatgtag actgagtcag gttaattatc tgacgaacgt attcagaatt catctgctcc
      721 ccgattaaaa gaagcgaact ttgtgtcaaa ctcccaccag acttatgcat ggaaactgct
      781 gccatatcag ctcctgcatc catagcagaa attggaagtt taccagtgaa atgcaaatgc
      841 gctccatggg cttcatctac taaaaccatc atgccagctt catgagccat ttctgtcaac
      901 ccctttaggt ctgaacagat gccgtagtaa gtaggattgt tgattaggat agccttagca
      961 tctggatggt cctttatggc ctgtgctact cggtcatttt caagacctaa agcgatacca
     1021 atcttaggat ctacactcat ctcgatatag atgggaatgg caccacatag aaccaacgca
     1081 ttgatagcag atttatggac atttcgtggc agaataatct tatctcctgc cttgcaggtt
     1141 gccagaatca tagtctgcac cgatgaagtt gttccaccaa tcattagaaa ggcatggcta
     1201 gctccaaaag catctgcagc cagctcctct gcatcacgaa taatcgaaat aggatggcct
     1261 aaattatcca agggtttcat cgaattgaca tcaatgccta cacatttttc tcctaagagt
     1321 tcgacaagtt ctggatttcc ccgtccacgc ttgtgacctg gaacatcaaa gggaacaatc
     1381 cttttcttgc gtaacttcac caaggcctca taaattgggg cttggttttg atctaactct
     1441 ttcaaaacat actcctttca atatttttag gaccttatga aaactaagga gctaaaggtt
     1501 gactcatgaa aaaagagcag gttttcacct gctctgcaat cttctgacgt gtttattttg
     1561 gtttctgttg agtatgtctg ttcccgatgt ttcctaactc tctagtagca aatattacgt
     1621 actttattga tcgtttcaca agatgacgtg tgctttcacc acactaaaaa tttatgaatt
     1681 aggacaagtg tcctaacatc aacttataaa agtaggcttt taaccctatc aataatgtgg
     1741 cttttcaacc acgcttcggc attcaaccct gcctgtccgt aggcattttt tactcgggtt
     1801 tatatttgct agaaaacatc atctcatttt ctaagcttta ttactatatc atgtttttaa
     1861 gaacttgtaa agtatttttt gataaaaaac aaaaatatag tagattgaaa ctagaatagt
     1921 acacctctgc ttctaaaaca ttgttagaaa tagattttac tgtcctgatc gatttgttct
     1981 gttcttgttt cattttacta tatgttcgta aatgaaagtt ctataatgaa gttagccacc
     2041 cagccctcct aaaaattaga tggttcaact agctga
//
LOCUS       DBERYS010000094         1843 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.094, whole genome shotgun sequence.
ACCESSION   DBERYS010000094 DBERYS010000000
VERSION     DBERYS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.094"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(4..1176)
                     /locus_tag="ACL8LA_002020"
                     /pseudo
     CDS             complement(4..1176)
                     /locus_tag="ACL8LA_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730289.1,RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1157..1351)
                     /locus_tag="ACL8LA_002021"
     CDS             complement(1157..1351)
                     /locus_tag="ACL8LA_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1813713.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1521..>1843)
                     /gene="tnpB"
                     /locus_tag="ACL8LA_002022"
     CDS             complement(1521..>1843)
                     /gene="tnpB"
                     /locus_tag="ACL8LA_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1813714.1"
                     /translation="VYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQVFLFCGGRKDR
                     FKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFSITPKINLSES
                     RDFY"
BASE COUNT          526 a          401 c          333 g          583 t
ORIGIN      
        1 ctataattga tgacgtttag ctgtttccaa caaactcata ataatagctc ttgctttagc
       61 tccttcaaaa ctttgagaaa ataaccaccc tcgctttttt aaactgagct taggctaaaa
      121 gagtccactg gactctttta ctccgtccca taaccaatga tttaatggcg cattcagcta
      181 gattattgga aaggaccaga tgtccgtctt tcaaaatagt cttaaaggtt tctttatact
      241 tgaggctata cttgaggctg tattcaattg cccttcctag ttttgaaccc gataaaactg
      301 actgacgccg gcaccaagca aagaaatctt ccattagggg ttggagatgt tcttgacgtt
      361 tctgtagtcg ttcatcagct ggcaaagcct cccagtttct ttccaaggaa aataactgat
      421 tacaataagc taaaccttta gctcctaagg atgatttatt cgcttgcttg ggggtcgctt
      481 caaaaaactt ccttctcaca tgtacccaac atccaacaag ttcagcttct tccagttgac
      541 aataagcttc tagcggttcg cagtttacca agcttagcgt cgccttgctc ctaaaccttg
      601 gactgctatc gcataggcag aactagagga ctaaagtcct aagttactgc cgcaacatat
      661 cacaatgcac atagccagaa taatctccta ggaattcttg cactaccgaa ccactccgac
      721 gctgatcatg gtggtacagc gtgattgctt gattctcagc tttcccagac aaaaaggtcc
      781 aatagtaggt cagatggcta tcattctcta gaacccgata ggaggtttca tctgcatgaa
      841 gtaagggctg aatcaatagt ctcgttataa aggggcttta ggaataggag cttttacaat
      901 tttatcactt ggattattct tactgcatgt ttggcatttg taagcgtgtt ggatatgatc
      961 cactcttttt aattgcgcag gaataaagac caattcttgt cgttgaaggc tcgctccaat
     1021 ctcttttaaa tcgtcctgac aatcagggca aatgctctct tctacttgat gatgaacttc
     1081 ttctgaatca aattgggcaa gaatagcttg acgtttccct ttagatttct tacgtttgta
     1141 ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt ctcagatgga gactcttcct
     1201 caaaaaggtt gagttgtcca aaagggcaaa cacttttctc agaggatttt ccagagagct
     1261 tttgcgttag ataagccact tgttcacaca gaagggaaag ttcattagtg agactatcaa
     1321 ttatagcact ctgttgttga atgattttca atcttctccc atagactttc ctccttattt
     1381 cctaacttta ttatatagtg tattgaatct ataacagtac accttgactg ctaaaatatt
     1441 tctataaatt aatttgactt tcctgataga gatgttcata tcttatttca attcactata
     1501 ctaaaaagaa agtcctcatt tcaatagaaa tcacgacttt ctgataaatt tattttggga
     1561 gtgatagaaa agcccttcat aagccaatct acttgttcag gtgtgagagc tttgacatcc
     1621 ttttctgtac tgggccaagt cagtctgccg ttctcaaagc gtttatatag tagccaaaat
     1681 ccttgaccat cccagtaaag ggctttaaag cggtctttac gtccaccaca aaagagaaaa
     1741 acttgaccgg agaaaggatc caattcaaag tgggttttaa ccagataagc cagtgaatcg
     1801 attccttgtc tcatatcagt cttcccacac acgagataga cct
//
LOCUS       DBERYS010000095         1705 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.095, whole genome shotgun sequence.
ACCESSION   DBERYS010000095 DBERYS010000000
VERSION     DBERYS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.095"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..86
                     /locus_tag="ACL8LA_002023"
     CDS             <1..86
                     /locus_tag="ACL8LA_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS1813715.1"
                     /translation="PILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            134..1609
                     /locus_tag="ACL8LA_002024"
     CDS             134..1609
                     /locus_tag="ACL8LA_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGS1813716.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          478 a          345 c          345 g          537 t
ORIGIN      
        1 ggccaatctt attccgtatc ccagcaatga gcgctccagt atttgacaat attgaagcag
       61 ctattaaaga acatggatta agctaattct aatttgttaa cattcatata acataaaaaa
      121 gggaggaact gttatggatg caatctttga cctaatcgga aaggttttca atcccatctt
      181 agaaatgggt ggacctgtca tcatgttgat cattttgaca gtattggctt tactttttgg
      241 agtgaaattc tccaaagcgc ttgaaggtgg tatcaaactt gccatcgctc ttacaggtat
      301 cggtgctatc atcggtatgc taaatggtgc tttctcagca tcacttgcaa aattcgttga
      361 aaacactggt atccaattga gtattacaga cgttggttgg gcaccactcg caacaatcac
      421 ttggggttct gcttggacac tatacttctt gctcatcatg ttgattgtca acgtagtgat
      481 gcttgctatg aagaaaaccg atacacttga tgtcgatatc tttgatatct ggcacttgtc
      541 tatcacaggt ctcttgatta aatggtatgc tgataacaat ggtgtgagtc aaggggtttc
      601 actctttatt gctacagcag ctatcgtcct tgtcggtgtg ttgaaaatta tcaactctga
      661 cttgatgaaa cctacatttg atgaccttct taacgcccca agttcatcac caatgacatc
      721 aactcacatg aactacatga tgaacccagt tatcatggtt ttggataaga tttttgaaaa
      781 attcttccca ggccttgata aatatgactt tgatgctgct aaattgaaca agaaaatcgg
      841 tttctgggga tctaaattct tcatcggttt catccttggt atcgttatcg gtattatggg
      901 aactccacat ccaattgcag gtgttgcaga tgcagataaa tggcgtcttg ttatcaaagg
      961 atggttgtct cttggtttga ctgccggtgt atctttggaa ctcttctcac ttatcggttc
     1021 atggttcatc gcagccgtag aaccactatc acaaggtatt acaaacgttg ctactaaacg
     1081 tcttcgagga cgtaaattca atatcggtct tgactggcca ttcatcgctg gtcgtgctga
     1141 aatctgggct tgtgccaacg tacttgcacc aatcatgttg attgaagcag tgcttctttc
     1201 aaaagttgga aatggtatct tgccacttgc aggtatcatc gctatgggtg ttactccagc
     1261 tctcttggtt gtaactcgtg gtaaattgct ccgtatgatt atcttcggaa cactcttgtt
     1321 gccactcttc cttctttcag gtacacttat tgcaccattt gcaacagaac ttgctaaagg
     1381 tgtaggtgcc ttcccagaag gtgtgagcca aactcaattg attactcact ctactcttga
     1441 aggaccaatc gaaaaacttc ttggttggac aattggtaac actacaactg gtgatatcaa
     1501 agcaatcctt ggtgcagtag tcttccttgt attctatatc ggtatctttg cttggtacag
     1561 aaaacaaatg atcaaacgta acgaagagta cgcagcaaaa gcaaaataat gcgctcctat
     1621 aaaatataaa ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctaaa
     1681 aacacagttt tgaggttgta gataa
//
LOCUS       DBERYS010000096         1662 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.096, whole genome shotgun sequence.
ACCESSION   DBERYS010000096 DBERYS010000000
VERSION     DBERYS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.096"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            146..1492
                     /locus_tag="ACL8LA_002025"
     CDS             146..1492
                     /locus_tag="ACL8LA_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HGS1813717.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          491 a          359 c          325 g          487 t
ORIGIN      
        1 tctaaaaacc atccagaatc cttgccttag cttagatcct ggatggtttc ttttttcacc
       61 caatgggtgt tttttactag acaaaaaaga gtttcccctt tatggtataa gtgtagaaaa
      121 aaacacaaaa agaaaggaaa ctcacatgaa cagtttacca aatcatcact tccaaaacaa
      181 gtctttttac caactatctt tcgatggagg tcatttaacc cagtatggtg gtcttatctt
      241 ttttcaggaa cttttttccc agctgaaact aaaagagcgg atttctaagt atttagtaac
      301 gaatgaccaa cgccgctact gtcgttattc ggattcagat atccttgtcc agttcctctt
      361 tcaactgtta acaggttatg gaacggacta tgcttgtaaa gaattgtcag ctgatgccta
      421 ctttccaaaa ttattggaag gagggcagct tgcttcacag ccaaccttat cccgttttct
      481 ttccagaact gacgaggaaa cagtccatag tttgcgatgc ctcaaccttg aattggtcga
      541 attcttttta cagtttcacc agctaaacca actcattgta gatatcgatt ctacccattt
      601 cacaacttat ggcaagcaag aaggtgttgc ttataacgcc cactatcgtg ctcatggcta
      661 tcatcctctt tatgctttcg aggggaagac aggttattgt ttcaatgccc agcttcgtcc
      721 tggtaatcgt tattgttctg aagaggcaga cagctttatc acacctgttt tagaacggtt
      781 taatcaactt ctctttcgaa tggatagtgg ctttgcgacc ccaaaattat acgatttaat
      841 tgaaaaaaca gggcaatact acctcataaa actcaagaaa aatactgttc tgagccgtct
      901 tggagacctt tccctccctt gcccacagga tgaggactta accatcttgc cccactccgc
      961 ctactcagaa actctctatc aagcaggatc ttggtcgcac aagcgtcgtg tctgccagtt
     1021 ctctgaacga aaagaaggaa acttgttcta cgatgttatt tctctcgtta caaatatgac
     1081 gagtggaaca agccaagacc agtttcagct ttatcgtgga cgtggtcaag ccgagaattt
     1141 catcaaggag atgaaggagg gattttttgg cgataaaacg gatagttcaa ccttaatcaa
     1201 aaacgaagtt cgtatgatga tgagctgtat cgcctacaat ctctatcttt ttctcaaaca
     1261 tctagctgga ggtgacttcc aaactttaac aatcaaacgc ttccgccatc tttttcttca
     1321 cgtggtagga aaatgtgttc gaacaggacg caagcagctc ctcaaattgt ctagtctcta
     1381 tgcctattcc gaattgtttt cagcacttta ttctaggatt agaaaagtca acctgaatct
     1441 tcctgttcct tatgaaccac ctagaagaaa agcgtcgtta atgatgcatt aaagaacagt
     1501 cgagatgaaa aaatcgtgtg acgaaccaag ggaggagtct gcccttttga ggaaatctag
     1561 cgaggaaaaa cgatactgga acagcagaaa gtaaaactga cctcatgagg aggaagaaag
     1621 tggctcatga ggtcaggggt tttgtaagtt acatctagtt ga
//
LOCUS       DBERYS010000097         1653 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.097, whole genome shotgun sequence.
ACCESSION   DBERYS010000097 DBERYS010000000
VERSION     DBERYS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.097"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            35..491
                     /locus_tag="ACL8LA_002026"
                     /pseudo
     CDS             35..491
                     /locus_tag="ACL8LA_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            656..955
                     /locus_tag="ACL8LA_002027"
     CDS             656..955
                     /locus_tag="ACL8LA_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGS1813718.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="ACL8LA_002028"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="ACL8LA_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1813719.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          563 a          327 c          288 g          475 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatca atttgtcctg
     1621 ttcttgtttc attttactat agaacgattt gaa
//
LOCUS       DBERYS010000098         1501 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.098, whole genome shotgun sequence.
ACCESSION   DBERYS010000098 DBERYS010000000
VERSION     DBERYS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.098"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="ACL8LA_002029"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="ACL8LA_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS1813720.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          466 a          316 c          307 g          412 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacatg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggttct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt ctttttgatg ttcagggcga
     1501 t
//
LOCUS       DBERYS010000099         1363 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.099, whole genome shotgun sequence.
ACCESSION   DBERYS010000099 DBERYS010000000
VERSION     DBERYS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.099"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(299..481)
                     /locus_tag="ACL8LA_002030"
     CDS             complement(299..481)
                     /locus_tag="ACL8LA_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813721.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(483..1262)
                     /locus_tag="ACL8LA_002031"
     CDS             complement(483..1262)
                     /locus_tag="ACL8LA_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813722.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          449 a          295 c          199 g          420 t
ORIGIN      
        1 aacatatata ctgcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tgattttact gcttatatta taccatatgg agagagatta aaaaactagc agtggagttc
      121 ctgctagctc tctgcgactt ggattaccat ctagataagt ttaaagagcc aatccaatat
      181 cttaaacaat atcttgtaaa acagcaattg aactgcaaag gaaatgatca tccaaaagaa
      241 tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagaccaa aaaaaatttc
      301 attctctctc ctctggccta atgagccacc gagccgtatc cattagctta tctgcaataa
      361 aaagttttcc cagaccaaca acgacatggt cagctttctt tatcgttttc atcactttta
      421 caccttgcac ggtcaaaaag taattaccat aaattttatc aaaagctttg ataggattca
      481 tattactctc ctttaatgat ttcagcctct tttcggattg tttcaacatc ttcttgatat
      541 tgaacttcac tatagttgac tagcttggat aattctttct gcaagctttc ccccattggt
      601 ttcatagttg caaaggttaa accacctgca atgataccac ccaagatagg aataaatttc
      661 cctattcctt tggccagccc tcccttggta agattcacac caaatatttt taagactttt
      721 ttcaaaatag ggtaccaaag cgtctttgtt aaagctttat taggcactgt tttcattacc
      781 tgtttggcaa ttgttatacc accagcacat agcaaagcag cggttccatt cacccctaac
      841 attacgccta gataaagcaa gagggtattt tgagcatctt cactcaactc ctcgcgtgaa
      901 gcccaaagat cctcataacc ataaatataa cctaattctt gagccaattt cagagagaaa
      961 gcataaaatt gagccacatc agctggaatg gtaattgcca tagcaagccc tccagctaat
     1021 cctgccaaaa cagaagtccc actcgctaat aatacattgt cccgaataca agcattagct
     1081 acacgatcta atatttcttg agatagaaga gtcgttgtac cttgttccaa taaggtagga
     1141 atatcttttg gatccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc
     1201 ttaacaacag gtagtttcac cacttgttgc aatacttgca tagctaagtc ttgttcagcc
     1261 atatacaaac tccttatttc ttttttacac cttatcatat tatatttcct acaaaaaaag
     1321 cattttctaa ttagataaca ttaaaaaata cagaacccct ttt
//
LOCUS       DBERYS010000100         1147 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.100, whole genome shotgun sequence.
ACCESSION   DBERYS010000100 DBERYS010000000
VERSION     DBERYS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.100"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..107)
                     /locus_tag="ACL8LA_002032"
     CDS             complement(<1..107)
                     /locus_tag="ACL8LA_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1813723.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASL"
     gene            complement(552..950)
                     /locus_tag="ACL8LA_002033"
     CDS             complement(552..950)
                     /locus_tag="ACL8LA_002033"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1813724.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          342 a          222 c          175 g          408 t
ORIGIN      
        1 agagaggcaa tttctctatt tgatttccct tcttttttcc atctttcgat taagcgacgg
       61 ctatcgattg tcaaatgttt ggcttttgta gtataattgt cttgcatctc tgtgcctttc
      121 ttgtgtttgt ggttgaacaa caagtataac acagaggtgt tttcttatgc ctacaagagc
      181 tttcattatt tccattttct tttggatttc actctattct gaaaaacttg tgtatattta
      241 ctgaagctag caagtcttac ctgtaaattt aatgaaagca acacaaaatc cgagagggga
      301 atctcggatt aatagataga gagtttttag tttaaataaa ttgtttaaaa tatcaacaac
      361 atcacttctt ttcttaacct gataagtctt gattcctaat tttggggcta cgattatatt
      421 gtcctcaata tcgtctagaa agacacaatt tctaggttat aactggtatt tatcgatagt
      481 tactcatata catcagtcca cctccatact tatgtgcgag cctctctttg tattatacct
      541 ccatactcac cttacagatt cttttggtaa taatatcttt gcctaatgta gagatagtct
      601 tgcaaagaaa aaacttcctt gtagccatgt ttctgataaa agtccggtgc ctggaactgg
      661 taagtattga caaaggcaaa acaacaattt cgattcttag cttcactttc tgcctgttgc
      721 aatagttttg aaccgattcc ttgccctcgc agttcctctt ttacaaacaa atactcgatt
      781 tctagccaat ttccaaaagt ctctgctatc aaacctgcca ggagattgcc cttttcatct
      841 tcgacataaa gattaagtgg ctcactttca gcctcttctc tttttgaacg gttataaaca
      901 cgaatcagat tccctatttc ttgcgattta tgtgattcct tattttccaa tctaaagtat
      961 agtgaaatga aataaaacat gcgcaaatcg attaaggaat ttaatctaat ttctaacaat
     1021 gtcttagaaa tcaaagtgta ctattttaac ttcaatgcac tatacatcta atactcaata
     1081 aaaatcaaag agcaaactag gaaactagcc gcaggttgct caaaacactg ttttgaggtt
     1141 gtagata
//
LOCUS       DBERYS010000101         1109 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.101, whole genome shotgun sequence.
ACCESSION   DBERYS010000101 DBERYS010000000
VERSION     DBERYS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.101"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(779..1039)
                     /locus_tag="ACL8LA_002034"
     CDS             complement(779..1039)
                     /locus_tag="ACL8LA_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813725.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
BASE COUNT          375 a          208 c          180 g          346 t
ORIGIN      
        1 aactaggatt ctagttttgc aggaagtcta ttatatatat gcacagacat atgaaatctt
       61 tttgatacag ccttcttttt acatagagag acagcagtta tgatatattt atccattcac
      121 aaaatcttct gtaccaatac tacaataatt ggtacatttt gattacacaa tcgtttcaaa
      181 aaatatgatg gaagactgag tattttatag taattccctt cctatcaact agatcaacca
      241 gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag gactatgatc agcatttcct
      301 agccaatagg acgctccaaa tcacctattc taagtccatc actagcttgg tcagtaagat
      361 acagactgca aggcaagact gacgtggttg atgagatttt ggaagagtat tacaatcata
      421 gattcaccag tagacttgac cgcagattta actatagtag attgaaacta gaatagtaca
      481 cttctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt
      541 ctcgtttcat tttactatac taaaaaacca cctgaaacaa atcaagtgac tagaggataa
      601 aaacaaagac tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca ggtcttgaaa
      661 aaccttgcta ctatacgttt gattgtggga agattgactt cattttctcc tgaaattgaa
      721 gttttttcta gtttccaggt atagacgaat tcaatatagc catctcgcta caattatttt
      781 aaccatcgaa aaaatttttc agaatttcaa aaatttcatt ttgatccaat gaaatgtcat
      841 tattttgaag aatagttgac ttcacaggct ctaattctat cacaaagcgg tgcatgtctg
      901 cttttaccgc actacttgat gttatagccg ataacttttc atctcctagc aaggaagctg
      961 tcaaacgggt aaggtcattc aaatgctttt taatggactt acttaaacct tgagcacctg
     1021 tggcagagcg ctccctcata tccagccaag ctttagcctt aaaaataatt aatactcaat
     1081 gaaaatcaaa gagcaaacta ggaagctag
//
LOCUS       DBERYS010000102         1084 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.102, whole genome shotgun sequence.
ACCESSION   DBERYS010000102 DBERYS010000000
VERSION     DBERYS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.102"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            70..609
                     /locus_tag="ACL8LA_002035"
     CDS             70..609
                     /locus_tag="ACL8LA_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1813726.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            676..>1084
                     /locus_tag="ACL8LA_002036"
     CDS             676..>1084
                     /locus_tag="ACL8LA_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGS1813727.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLY
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
BASE COUNT          294 a          213 c          256 g          321 t
ORIGIN      
        1 gacgttgacg cggtttgaag agattttcga agagtataag aaaaaatcag tcccctaaag
       61 gagcagatta tgaagttatt gtctatcgcc atttctagct ataatgcagc agcctatctt
      121 cattactgtg tggagtcgct agtgattggt ggtgagcaag ttgggatttt gattatcaat
      181 gacgggtctc aggatcagac tcaggaaatc gctgagtgtt tagctagcaa gtatcctaat
      241 atcgttagag ccatctatca ggaaaataaa ggccatggcg gtgcggtcaa tcgtggcttg
      301 gcagaggctt ctgggcgcta ttttaaagta gttgacagtg atgactgggt ggatcctcgt
      361 gcctacttga aaattcttga aaccttgcag gaatttgaga gcaagggtca agaggtggat
      421 gtctttgtga ccaattttgt ctatgaaaag gaaggtcagt cttgtaagaa gagtatgagt
      481 tacgattcag tcttgcctgt tcggcagatt tttggctggg accaggtcgg aaatttctcc
      541 aaaggccagt atatcatgat gcactcgctg atttatcgga cagatttgtt gcgtgctagc
      601 cagttctaat tgcctgaaca tactttttat gtcgataatc tctttgtctt tacgcccctt
      661 cagcaggtca agaccatgta ctatctgcct gtcgatttct atcgttattt gattgggcgt
      721 gaggaccagt ctgtcaatga gcaagtgatg attaagtgca ttgaccagca actcaaggtc
      781 aatcgactct tggtagacca actttatttg tcccaagtga gtcatcccaa aatgcgagaa
      841 tatctgctga atcatattga aatcacgacg gtgatttcca gtaccctgct caaccgatct
      901 ggaacagcgg agcatctggc aaaaaaacgc caattgtgga cctatattca gcagaaaaat
      961 ccagaagtct ttcaggctat tcgtaagacc atgttgagcc gtttgaccaa acattctgtc
     1021 ttgccagatc gcaaactgtc caatgtcgtc tatcaaatca ccaaatctgt ttatggattt
     1081 aatt
//
LOCUS       DBERYS010000103         1038 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.103, whole genome shotgun sequence.
ACCESSION   DBERYS010000103 DBERYS010000000
VERSION     DBERYS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.103"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            119..>1038
                     /gene="pavB"
                     /locus_tag="ACL8LA_002037"
     CDS             119..>1038
                     /gene="pavB"
                     /locus_tag="ACL8LA_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGS1813728.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVK"
BASE COUNT          353 a          188 c          216 g          281 t
ORIGIN      
        1 taatataagt gttttataag agggatttaa gaaaaatttt aactttttct tagtcctttt
       61 taatttcagg agattatact agagtcatca aataaagaaa gactctaagg agaatcctat
      121 gaaattcaat ccaaatcaaa gatatactcg ttggtctatt cgccgtctca gtgtcggtgt
      181 tgcctcagtt gttgtggcta gtggcttctt tgtcctagtt ggtcagccaa gttctgtacg
      241 tgccgatgtg gtcaatccga ccccaggtca agtcttacct gaagagacat cgggaacgaa
      301 agagggtgac ttatcagaaa aaccaggaga caccgttctc actcaagcga aacctgaggg
      361 cgttactgga aatacgaatt cacttccgac acctacagaa agaactgaag tgagcgagga
      421 aacaaactct tctagtctgg atacactttt tgaaaaagat gaagaagctc aagaaaatcc
      481 agagctaaca gatgccttaa aagaaactgt agatacagct gatgtggatg ggacacaagc
      541 aagtccagca gaaactactc ctgaacaagt aaaaggtgga gtgaaagaaa atacaaaaga
      601 cagcatcgat gttcctgctg cttatcttga aaaagctgaa gggaaaggtc ctttcactgc
      661 tggtgtaaac caagtaattc cttatgaact attcgctggt gatggtatgt taactcgtct
      721 attactaaaa gcttcggata atgctccttg gtctgacaat ggtactgcta aaaatcctgc
      781 tttacctcct cttgaaggat taacaaaagg gaaatacttc tatgaagtag acttaaatgg
      841 caatactgtt ggtaaacaag gtcaagcttt aattgatcaa cttcgcgcta atggtactca
      901 aacttataaa gctactgtta aagtttacgg aaataaagac ggtaaagctg acttgactaa
      961 tctagttgct actaaaaatg tagacatcaa catcaatgga ttagttgcta aagaaactgt
     1021 cgaaaaagct gttaaaga
//
LOCUS       DBERYS010000104          966 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.104, whole genome shotgun sequence.
ACCESSION   DBERYS010000104 DBERYS010000000
VERSION     DBERYS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.104"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>966
                     /locus_tag="ACL8LA_002038"
     CDS             <1..>966
                     /locus_tag="ACL8LA_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HGS1813729.1"
                     /translation="LDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCPDCGSLMKKYD
                     FQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQIPRIINQKIAQ
                     KLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIA
                     QDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAK
                     IVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYR
                     PTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKF"
BASE COUNT          307 a          207 c          156 g          296 t
ORIGIN      
        1 tgctcgatat taaagaccca aacatcaaga ttctagatat catcaatatg gatacccaca
       61 aagaaattat cgctaagctg gattatgagg ctccatcttg ccctgattgt ggaagtctaa
      121 tgaagaaata tgactttcaa aaaccgtcta agatccctta cctcgaaaca actggtatgc
      181 cttctagaat tctccttaga aaacgccgtt tcaagtgcta tcactgttca aaaatgatgg
      241 ttgctgagac tcccctggta aagaaaaatc accaaatccc tcgtatcatc aaccaaaaaa
      301 ttgctcaaaa gttaattgaa aaaatttcta tgactgatat tgcccatcag ctggccattt
      361 caacttcaac tgtcattcgc aagctcaatg actttcactt tgagcatgat ttttctcggc
      421 ttccaaagat tatgtcttgg gatgagtatg ccttcactaa gggaaagatg agtttcattg
      481 cgcaagattt tgataatctt aatattatca ctgttcttga aggcagaaca caagctgtca
      541 tccgaaatca ctttcttcgc tacgatagag ccgttcgttg tcaagtgaaa atcattacga
      601 tggatatgtt tagtccttac tatgacttgg ctaaacagct ttttccgtgt gctaaaatcg
      661 ttctagatcg tttccatatt atccaacatc tcagccgtgc catgagtcgt tttcgtgttc
      721 aaattatgaa tcagtttgaa cgaaaatctc atgaatacaa ggctatcaag cgttactgga
      781 aactcatcca acaggatagt cgtaaactca gcgataaacg tttttatcgc cctacttttc
      841 gcatgcactt aacaaataaa gaaattcttg acaagatttt aagctattca gaagacttga
      901 aacaccacta tcagatctat caactcttac tttttcactt tcagaacaaa gaccctgaga
      961 aatttt
//
LOCUS       DBERYS010000105          856 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.105, whole genome shotgun sequence.
ACCESSION   DBERYS010000105 DBERYS010000000
VERSION     DBERYS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.105"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            231..706
                     /locus_tag="ACL8LA_002039"
                     /pseudo
     CDS             231..706
                     /locus_tag="ACL8LA_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            744..>856
                     /locus_tag="ACL8LA_002040"
     CDS             744..>856
                     /locus_tag="ACL8LA_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS1813730.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAV"
BASE COUNT          295 a          129 c          164 g          268 t
ORIGIN      
        1 gtgttttgag taacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
       61 gaacagaaaa atgcttggag tatttgtttg tgtgtttatt tttatataac aaactataaa
      121 cacaacaaaa atataaaaaa agagacaaaa aagaacagaa agtaattaac aacgatttca
      181 tatagtgtta tacttatagc ataaagttaa tggaaagaag ggaaaacctt atggtattag
      241 attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa gaataactct
      301 ttgatcaggt agcaagcttg ttggaggaac gagaaattgt aactccaact tatcgtgaag
      361 ctttgatcac gcgtgaaaag tcatttccaa ctggtttaga tatggaattt ctaggaaagg
      421 acttgcaaat gtaatgattc ctcacacaga tattgttcat aatctagctg agaaagtggt
      481 ggttgttcga ttagagaaac cggtgacttt ccataatatg attgcgccag gtaaggaagt
      541 agaagtatcc ttgctcttct ttatcatcaa taattcaagt tcaagtcaaa cgaacattct
      601 tgctcagttg atggactttt tcacaggaaa tggacatctt gaagacctat caaaaatttc
      661 cgaaccagaa aaactttatg cttatataga tgaagcaatc gcttaatctt gtctattaaa
      721 aaataaaatc ggaggaagtc tatatgatta aaattcttgc tgcctgcggt gcaggtgtta
      781 actcaagtca ccaaattaaa agtgctctag aagaagaact ttcaaaccgt ggttacgatg
      841 ttcactgtga tgcagt
//
LOCUS       DBERYS010000106          813 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.106, whole genome shotgun sequence.
ACCESSION   DBERYS010000106 DBERYS010000000
VERSION     DBERYS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.106"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>813
                     /locus_tag="ACL8LA_002041"
     CDS             <1..>813
                     /locus_tag="ACL8LA_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813731.1"
                     /translation="GLQGRDGEQGPKGEDGKNPTVKVTDGQDGTHTITINDGKGGITT
                     TVVRDGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGP
                     KGDKGDNGKDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAER
                     DDAKKQTTLTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVD
                     PTTEGKDGDTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQG"
BASE COUNT          348 a          131 c          187 g          147 t
ORIGIN      
        1 aggtcttcaa gggcgtgatg gtgaacaagg accaaaagga gaagatggga aaaacccaac
       61 agttaaagta actgatggtc aagatggaac gcatacaatt acaattaacg atggtaaagg
      121 tggtataact actacagtag taagagatgg atttgatggt gcaagccctc ttgtttctac
      181 tcatagaaat gaagcagata aaacaacaac tgttattttc tattatgatc taaatgataa
      241 taatcaattt gatgaaggag atacaaaact taaagaagtt gttatcgcag atggaaaaca
      301 aggaccaaaa ggtgacaaag gtgataacgg aaaagatgga ttcacaccag aagtaacagt
      361 tacagataac aataacggaa cacacaccat cacaatcaca caaccagaca acagaccatc
      421 attaacaaca atcgttaaaa acggtgaaga tggaaaaaca ccaaaagtca aagcagaacg
      481 agatgatgcg aagaaacaaa caacattaac attctacatt gacaaagatg gagatggaag
      541 ttacacagca ggaaaagacg agttagttca aacaacagta gttaaagacg gacaagacgg
      601 agctgcagga gcatctggac gtgatggaaa agaagtatta aacggaaaag tagacccaac
      661 aacagaaggt aaagatggag acacattcgt aaatacacaa acaggagatg tattcgttaa
      721 gaaaggtaac acttgggaac cagcaggaaa catcaaagga ccgaaaggtg acaaaggtgc
      781 agatggtgcc aagggtgaaa aaggagccca agg
//
LOCUS       DBERYS010000107          772 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.107, whole genome shotgun sequence.
ACCESSION   DBERYS010000107 DBERYS010000000
VERSION     DBERYS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.107"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            82..561
                     /locus_tag="ACL8LA_002042"
     CDS             82..561
                     /locus_tag="ACL8LA_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGS1813732.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            656..727
                     /locus_tag="ACL8LA_002043"
     tRNA            656..727
                     /locus_tag="ACL8LA_002043"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:689..691,aa:Glu,seq:ttc)
BASE COUNT          292 a          105 c          181 g          194 t
ORIGIN      
        1 ggagctaaag ggaacagaat ggagaaaatg gaacaaatgt gttttctaat ctgttagact
       61 gtatctagaa aggggaaaat tatgattaaa gaattgtatg aagaagtcca agggactgtg
      121 tataagtgta gaaatgaata ttaccttcat ttatgggaat tatcggattg ggaccaagaa
      181 ggcatgctct gcttacatga attgattagt agagaagaag gactggtaga cgatattcca
      241 cgtttaagga aatatttcaa aaccaagttt cgaaatcgaa ttttagacta tatccgtaag
      301 caggaaagtc agaagcgtag atacgataaa gaaccctatg aagaagtggg tgagatcagt
      361 catcgtataa gtgagggggg tctctggcta gatgattatt atctctttca tgaaacacta
      421 agagattata gaaacaaaca aagtaaagag aaacaagaag aactagaacg cgtcttaagc
      481 aatgaacgat ttcgagggcg tcaaagagta ttaagagact tacgcattgt gtttaaggag
      541 tttactatcc gtacccacta gtaagtcatg caaaaaaaaa tgaaaaaaat tagaaaaagt
      601 agttgacaaa gtttgaaaag actgtataat agtaagagtt gaaaataaca actcaggtcc
      661 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
      721 cggactatgg tatgttgcgt caggaccact tgatgaaaaa aagtttaaaa aa
//
LOCUS       DBERYS010000108          765 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.108, whole genome shotgun sequence.
ACCESSION   DBERYS010000108 DBERYS010000000
VERSION     DBERYS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.108"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(74..147)
                     /locus_tag="ACL8LA_002044"
     tRNA            complement(74..147)
                     /locus_tag="ACL8LA_002044"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="ACL8LA_002045"
     tRNA            complement(157..242)
                     /locus_tag="ACL8LA_002045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="ACL8LA_002046"
     tRNA            complement(250..321)
                     /locus_tag="ACL8LA_002046"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="ACL8LA_002047"
     tRNA            complement(344..416)
                     /locus_tag="ACL8LA_002047"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="ACL8LA_002048"
     tRNA            complement(435..516)
                     /locus_tag="ACL8LA_002048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="ACL8LA_002049"
     tRNA            complement(522..594)
                     /locus_tag="ACL8LA_002049"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="ACL8LA_002050"
     tRNA            complement(619..691)
                     /locus_tag="ACL8LA_002050"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
     gene            complement(694..765)
                     /locus_tag="ACL8LA_002051"
     tRNA            complement(694..765)
                     /locus_tag="ACL8LA_002051"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Val,seq:tac)
BASE COUNT          195 a          225 c          168 g          177 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt atcttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ctttgggagt taacgggatc gaaccgctga
      721 ccctctgctt gtaaggcaga tgctctccca gctgagctaa actcc
//
LOCUS       DBERYS010000109          758 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.109, whole genome shotgun sequence.
ACCESSION   DBERYS010000109 DBERYS010000000
VERSION     DBERYS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.109"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            339..665
                     /locus_tag="ACL8LA_002052"
     CDS             339..665
                     /locus_tag="ACL8LA_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1813733.1"
                     /translation="MILWGFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDG
                     FDYEEW"
BASE COUNT          255 a          115 c          157 g          231 t
ORIGIN      
        1 ctagcttcct agtttgctct ttgattttca ttgagtataa aaacaagaac accttgcttg
       61 gcgcagggtg tttcgcgtga aacagaagaa ttatctggtt tcaaatgcta cagttagaca
      121 aacttatgat aaaatagcag aaagtgaatg tttcctaaga gcaattctat cgatgagcaa
      181 ttggaggtat tatgctacac ttaaaattag taaaacaaga aatagaagct gaaaagccag
      241 catctgtaga agcttggatc atttccgtca aatttaaaaa aggttgctac cgacatatat
      301 agattccaaa aacaaaaacg ttagcggaac tagcagatgt gattttatgg ggttttgatt
      361 ttgcaaatga tcatgctcac gcatttttca tggataatgt tgagtggagt catgcagatt
      421 cttactttct tagctttgtt agtgacgatg ttgaagaacg ttacacagaa aatgtctatc
      481 tggatagcct aagtgtcaaa caaaaattta agtttatttt cgacttcggt gatgaatggc
      541 gttttgaatg ccaagtactg agagaaatcg agacagagga cgaagaagct tatctcgtac
      601 gttcggttgg aacgtcgcca gaacaatatc cagattatga tggttttgac tatgaagaat
      661 ggtaaaattg aaatctaaaa aacaattgga atcagtctgt gtaggcttag tatttcaata
      721 gacttcctgc aaaactagaa tcctaattca tgattgat
//
LOCUS       DBERYS010000110          695 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.110, whole genome shotgun sequence.
ACCESSION   DBERYS010000110 DBERYS010000000
VERSION     DBERYS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.110"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(41..637)
                     /locus_tag="ACL8LA_002053"
     CDS             complement(41..637)
                     /locus_tag="ACL8LA_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1813734.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
BASE COUNT          247 a          119 c           78 g          251 t
ORIGIN      
        1 aaaaagataa ataatcagat tcattaattt gtataaacta ttatttgttt gaaatttcat
       61 aaacccgtcc ttcctctatt aaaatccttt gagatttgct aatcaattct tgattcaata
      121 aattatgaga tacatatata atagtagagc tttctttcat ggaaattaag cgtttagcaa
      181 tcatatcaat acttcttgca tccactgttg ataaagtctc atcaataaaa atataatccc
      241 aatttttagc aaaaattaag gataagccta atttgaattt tgttccagta gatgctgtca
      301 tatagttatc tccgatttta ttatcaaaaa aagtattaat ttgttcttta tcctcaaatg
      361 atagatggat ttgaaaagaa tcacacaaca actgcaaatt ttcttctata gtaaatggaa
      421 caaccccaag ctgattggta tcaaggtata gtacatcttt ggagacacaa ttctttaaac
      481 cactatcaaa agtctctagc ccagctaaaa gttgtagaag ggtgctcttt cctgaaccat
      541 tctctccttc aactctgtaa attcccttat caatctctag tgatatattt ttcaatatac
      601 atttatcata acttttagat tcattttcaa ttttcataat ttaattccct ctatcaacgc
      661 tgctaaaatt aataaaagat aagaaatgat atacc
//
LOCUS       DBERYS010000111          606 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.111, whole genome shotgun sequence.
ACCESSION   DBERYS010000111 DBERYS010000000
VERSION     DBERYS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.111"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            21..383
                     /locus_tag="ACL8LA_002054"
     CDS             21..383
                     /locus_tag="ACL8LA_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGS1813735.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          207 a           71 c          111 g          217 t
ORIGIN      
        1 ttttcattga gtataacaca ttgttagaag ttggtttaaa tttcctaatc agtttgttca
       61 catttacctt cgatatatta tatcccatag ttaaggttgg tcatacagat gattatagcc
      121 atggagccgt aaaacttagt gtttctttag ttgacaaaga tgccatgaaa aaaatatctg
      181 taactgtaat aggatatttt gaaataaata tagatgaaaa tatcaccgat attctatacg
      241 taaatggtac tgctattctt tatccttatt tacgttctat tgtttcaata gtttcggcaa
      301 ttgatagcag tgaagcaatg ttgctaccta ccattaatgt tttagagtta ctagataaat
      361 ctcaaccttt tgaagaagaa taatttatta gctcactaaa ttgagggtaa ggaaaagtaa
      421 aagcagtaag aaaaatgtct tgcattatac agcaactttt tgggaatgag tagatggatt
      481 gaataaaatt tgattaagag tggatgattt atctgtagat tattattgga cagttagtct
      541 tgaagtagtc taagaattag gttataatca gtagaagcct tgctaataat gaggaggtta
      601 gtttat
//
LOCUS       DBERYS010000112          599 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.112, whole genome shotgun sequence.
ACCESSION   DBERYS010000112 DBERYS010000000
VERSION     DBERYS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.112"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..367)
                     /locus_tag="ACL8LA_002055"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="ACL8LA_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(474..599)
                     /locus_tag="ACL8LA_002056"
                     /pseudo
     CDS             complement(474..599)
                     /locus_tag="ACL8LA_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          167 a          147 c          117 g          168 t
ORIGIN      
        1 ttcagctaga ttattggaaa ggaccagatg tccgtctttc aaaatggtct taaaggtttc
       61 ttcatacttg aggctgtatt caattgccct tcctagtttt gaacctgcta aaactgactg
      121 acgccggcac taagcaaaga agtcttccat taagggctgg agatgttctt gacgtttctg
      181 tagtcgttca tcagctggca aagcctccca gtctctttcc aaggaaaata actgatcaca
      241 ataagctaaa cctttagctc ctaaggatga tttatccgct tgcttggggg ggcgcttcaa
      301 aaaacttcct tctcacatgc gcccaacatc caacaagttc agcttcttcc agttgacgat
      361 aagcagacga gcggttcgaa gtttaccaag cttagcgtcg ccttgctcct aaaccttgga
      421 ctgctatcgc ataggcagaa ctagaggact aaagtcctaa gttactgccg caaaatatca
      481 caatgcacat agccagaata atctcctagg aattcttgta ctactgaacc acttcgacac
      541 tgatcatggt ggtaaagcgt aatcccttgt ttctctgctt tacctgacaa aaaagtcca
//
LOCUS       DBERYS010000113          585 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.113, whole genome shotgun sequence.
ACCESSION   DBERYS010000113 DBERYS010000000
VERSION     DBERYS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.113"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..585
                     /locus_tag="ACL8LA_002057"
                     /pseudo
     CDS             1..585
                     /locus_tag="ACL8LA_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          172 a          122 c          108 g          183 t
ORIGIN      
        1 atgatggtcg ctgaaacttc tatcgtcaag aagaatcacc aaatccctcg tatcatcaac
       61 caaaagattg ctcaaaagtt aattgaaaag atttctatga ccgatattgc ccatcagctt
      121 tccatctcaa cttcaactgt cattcgaaag ctcaatgact tccactttga gtgtaatttt
      181 agaaatctgc ctgagattat gtcttgggac gttgaaacag tccggggagt gactgtttca
      241 atcgggagat ggagatgagc tttattgcgc aagattttga aaagctcaat atcatcactg
      301 ttcttgaagg tagaacacaa gctatcatcc gagatcactt tcttaaatat gatagagccg
      361 ttcgttgtca agtgaaaatc attactatgg atatgtttag tccttactat gacttagcta
      421 gacaacttcg ctttcaaatt tctaggctca ggctgaaaca gtctctcaga ctgtttcact
      481 cccgaatgct aaaatcgttc ttgatcgctt tcatattgta caacatctta gccgtgctat
      541 gagtcgtgtg catgtccaaa tcatgaatca gtttcatcga aagtc
//
LOCUS       DBERYS010000114          576 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.114, whole genome shotgun sequence.
ACCESSION   DBERYS010000114 DBERYS010000000
VERSION     DBERYS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.114"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            16..>576
                     /locus_tag="ACL8LA_002058"
     CDS             16..>576
                     /locus_tag="ACL8LA_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1813736.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRRTVRQCLGKGRFKEVYSADYAQQSYENNRKHSVKRSSVTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTR"
BASE COUNT          208 a          114 c          122 g          132 t
ORIGIN      
        1 aagaaaggca cagagatgca agacaattat actacaaaag ccaaacattt gacaatcgat
       61 agccgtcgct taatcgaaag atggaaaaaa gaaggaaaat caaatagaga aattgcctct
      121 ctacttggaa aagctcctca aactatccac actgaaatca agcgtaggac agtccgacaa
      181 tgtcttggaa aagggcgctt caaagaggtt tattctgccg actatgctca acagtcttat
      241 gaaaataatc gcaagcactc ggtcaagaga tcaagcgtga ccaaggaact aaaggaaaag
      301 attctccact atcataacca aaaattttcg cctgaaatga tggttatggc taaaggggtt
      361 aacgtgggaa tttcaaccat ttactattgg attcatcatg gaaaattggg gttaagcaag
      421 caggatctgc tttaccctag aaaaggaaaa gcgcttaaga aacaggctag caccaacttt
      481 aaacctgctg gtcaatccat cgaacagcgg cctgaagcta tcaatcttcg cttggagaat
      541 gggcattatg agattgatac ggttctactt acgaga
//
LOCUS       DBERYS010000115          527 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC16 isolate 456260
            SAMN38106771-rid24762883.denovo.115, whole genome shotgun sequence.
ACCESSION   DBERYS010000115 DBERYS010000000
VERSION     DBERYS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106771
            Sequence Read Archive: SRR26672998
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570232.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 13:39:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 2,024
            Genes (coding)                    :: 1,899
            CDSs (with protein)               :: 1,899
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 47 of 125
            Pseudo Genes (multiple problems)  :: 32 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106771-rid24762883.denovo.115"
                     /strain="GPSC16"
                     /isolate="456260"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>527
                     /locus_tag="ACL8LA_002059"
     CDS             <1..>527
                     /locus_tag="ACL8LA_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HGS1813737.1"
                     /translation="LQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKIL
                     HYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNF
                     KPAGKSIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKT
                     AESVNQALTLLLGEH"
BASE COUNT          188 a           95 c          105 g          139 t
ORIGIN      
        1 tttacaacaa gtgagaaaag ggctatacaa aaaggtctat tctgccgatt acgcacaaac
       61 tgtttaccaa ttcaatcgaa aacggtcggt gaaaaagtta attttaacaa aggaaatcag
      121 agagaagatc ttacactatc ataagcaaaa attttcgcct gaaatgatgg ttaacaagaa
      181 gcaagtgaaa gttggtattt caaccatcta ctactggttt cataatggtc atttaggatt
      241 gacgaaggcc gacatgcttt atcccagaaa aaggaaaggt gtcaagaagc aagctagtcc
      301 gaactttaag ccggcaggta aatctatcga agaacgtcct gacgttatta atcttcgctt
      361 ggaaaatggt cattatgaaa ttgataccgt cctactgact aagataaaaa attattgcct
      421 gttagtctta accgaccggc ggagtagaca ccaaattata aggttaattc caaataaaac
      481 tgctgaatct gtcaatcagg cgcttacgtt actattaggg gagcatc
//
